LOCUS       DAOOCX010000001        57439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_353_length_57439_cov_7.746306, whole genome shotgun sequence.
ACCESSION   DAOOCX010000001 DAOOCX010000000
VERSION     DAOOCX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 57439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57439
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_353_length_57439_cov_7.746306"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PK399_00005"
     CDS             complement(<1..608)
                     /locus_tag="PK399_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPW11542.1"
                     /translation="MGLIQAVAGAVGGMLGDQWKDFYTVPAGLPSTAALFAAVPQGTN
                     AGRGSNTSASSNIITNGSKIVVPEGYGLLLFQDGKITGFVAEPGGYEWRSDDINSKSI
                     FAGDGIVSPLIMQSWERFKFGGQPGSQQAAFYVSLKELPDNRFGTQSEIYWDDGFLNT
                     QVGAVTRGSYTLKIVDPILFVKNFVPASYLQPGKVFDFTDLD"
     gene            745..1830
                     /locus_tag="PK399_00010"
     CDS             745..1830
                     /locus_tag="PK399_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HPW11543.1"
                     /translation="MPDPNLDALRRYAVARTLFKPTTLARAIQRLGFLQADPIRSPAR
                     AQDLTLRLRVTGYRAGDLERRYPRLAVEEDCLVNYGFLPREHLALMHPREPRKAWDAA
                     TRRRADEVLAFVRERGPVHPREVDQHFAHGRVRNYWGGSSNASTQLLDGLHYRGLLRV
                     QRRDGGVRVYAATAHAPTDDSREGRARRAAALVDLVVRKYAPLPAASLTYLARLLGYG
                     APHLASEIRDALAQARARLPSCRIDGTTWYWSEGEDPAARRHAIDDTLRLLAPFDPVV
                     WDRRRFELFWGWTYKFEAYTPAAKRSFGYYALPMLWQGQVPGWANISLRGGRVEAEVG
                     YVGGAPRGRAFRAALDDELQRMTEFLA"
     gene            complement(1852..2445)
                     /gene="trmY"
                     /locus_tag="PK399_00015"
     CDS             complement(1852..2445)
                     /gene="trmY"
                     /locus_tag="PK399_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HPW11544.1"
                     /translation="MRTFVLRARAAPTDSRKLLDAVGQEAHTEILAHTLMNAIFVAQS
                     HRADVVVYLVLESTQDFSRTIRFEANAMHDIGGFDERSLLGKVARALDASRGMGKDEA
                     RPVECGVDVRTLSFERLVQELAQDHQLFVMDPKGTSIREQAFAGNPCFLLTDHIPMPK
                     KIFPSLERLGAKKLTLGSKMLFASQCVVLIHHELDQR"
     gene            2626..2997
                     /locus_tag="PK399_00020"
     CDS             2626..2997
                     /locus_tag="PK399_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11545.1"
                     /translation="MSFLFHRGLPAAIAVMALASVGCASQRSTAALAPGSQASLEGTI
                     ASIDTQPWTYDGNAVVLLDTTTHGQVPVQLPARWNLCKAASVDVAAMRTGQRVRAIGT
                     VIDDRALVVCERTEHRLQPLD"
     gene            3030..3395
                     /locus_tag="PK399_00025"
     CDS             3030..3395
                     /locus_tag="PK399_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6164 family protein"
                     /protein_id="HPW11546.1"
                     /translation="MAKLLLNLRHVPDDEADDVREMLDANGIAFYETKPGPWRISVGG
                     IWISQDAAIVEAKRLMAEYQLARGARARAEYAAAVRDGTAETFWSQLREEPLRVLLTV
                     VAIVLLLGLVALPVVLLRA"
     gene            3411..4166
                     /locus_tag="PK399_00030"
     CDS             3411..4166
                     /locus_tag="PK399_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HPW11547.1"
                     /translation="MFGPFGLVLLLALTGGCSGGAVQADIARPSVSPPRILVFTRTTG
                     WRHDSIPVAIETLRDLGEEAGLQVEHSEDPMQFSAATLARYRAVVFANTTLDVLDDAQ
                     QRAFERFVGAGGGYVGIHSAADTEYDWPWYGELVGAWFDSHPPGLQASPVRFEARHAL
                     PGEPDWQVRDELYNYRRNPRAWVDVIATVDESAYAGGKMGTDHPIAWCHQRMGGRAWY
                     TGLGHDIPLYADAGFRTHLLRGLRYATRQSDDC"
     gene            4215..4685
                     /locus_tag="PK399_00035"
     CDS             4215..4685
                     /locus_tag="PK399_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HPW11548.1"
                     /translation="MINNDILRSIRYMLDLSDNKVIEIIRLADPEFAIEKDELRGYLL
                     KEDEPGHVACGDRVMAHFLDGLVIYRRGRDESLPPRPVEKRINNNVVLKKLRVAFQFT
                     DVDMHRAFADAGFPVSKPELSALFRQPGHKNFRQCGDQLLRNFLKGLTLRMRGA"
     gene            4908..5678
                     /locus_tag="PK399_00040"
     CDS             4908..5678
                     /locus_tag="PK399_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11549.1"
                     /translation="MKRALVPVLVLASLAPMAAMAQDGPSFSLIAGRDVSVSGDVHKG
                     AISPIADLGPLNPALAGVSAELRIESRSYDDIYGNANTFGVEMSWPMAGGEVFGQLRS
                     SRSSDGEVQVGGAFVPALNTTLPVYGRFDGYRSLALEGGYRHYLSTGKLRPYVAGRLG
                     ATRVDDINATFSIPDANILISDVPFFDGGWNLSGGGDLGVMWALSDAASLGVEVGARY
                     HGDLDGNDSAIGGLGLASINNTGSRTSFPVSLRFQMRF"
     gene            5922..6806
                     /locus_tag="PK399_00045"
     CDS             5922..6806
                     /locus_tag="PK399_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW11550.1"
                     /translation="MALQLALESVGYRYRGGHEAVAGVGLTMAPGIVGLLGPNGAGKS
                     TLMRVLSTLAKPSSGRVLWRGEDIARKPDVLRRELGYLPQDFGVYEALSAREFLAFLS
                     AVKGLPSRTVAARVDACLEMVGLGDVADRRLGGYSGGMRQRVGIAQALLNDPKLLIVD
                     EPTVGLDPEERVRFRHLLAELAGERLVILSTHIVSDIEASAGMLAIMSGGRLRFHGTP
                     ETLMAQAEGHVWQWTLPAEQLAEARARLRISRAQRRAHGVEVRVVGDDAPAADAIPVA
                     PDLEDAYLWLLHRSGAQS"
     gene            6803..8347
                     /locus_tag="PK399_00050"
     CDS             6803..8347
                     /locus_tag="PK399_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11551.1"
                     /translation="MSGARAVAAILATDLRQRLRTPRFWLLIAGLAGLMWWCFPAIDR
                     DYLTVSVGEGMRGRYSSAWIGMVSALMYSSTLSVAGFYLVRGTVVRDLETRTWQLLVA
                     TTMSRGAYLLAKWLSHMVVFALLIGAGLAVGLVAQMLRAEDLRIDLLELVKPVLVLTM
                     PSLALTAALAVWFDLVPWLRRSAGNLLFFVVWVMLLTFGVSQAEQAPGAALPWPGDPH
                     GLLVAEYDLSHAWPTVDAGKELGLSVGSQALEGKAPVLLDWTHWQLSAATLRARGAWL
                     GLALVLLVLAVPFLDRCAAHVGNPRKALGDGARLRWLDAALSPLQRGTFGTLLAAEVR
                     LALRPRKAWWWLALLAAMGVQAFAPEKAMAVAVIVAWVLAMDVFARLVLREHETRTAA
                     LVFTAPGMRTRLLQVRALVAVGLAWLLVLPAILRLAVVNPQAALSALVVGASLAAWGM
                     ATGVLFRNGRMFELALLALAYIGVQGGLLLNVAVAPMDTLRWHLLGLPLALAILAIGW
                     PRMETR"
     gene            complement(8373..9095)
                     /locus_tag="PK399_00055"
     CDS             complement(8373..9095)
                     /locus_tag="PK399_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HPW11552.1"
                     /translation="MSDPVRLAKCVADLARCSRIEAEQYIKGGWVSVDGRVVEDPAYP
                     VTGESVSIDPAAQLETVEPATILLHKPVGYDTISGRKAAAALVQPDTRWAEDPSGVRM
                     LDRHFHRLTPLVPLDAEASGLMVLTQDGRVWRRLTEDGDEIEQEFVVEVSGEIAPYGL
                     RKLNHGLHYNGRALPPCKVSWQNEIRLRFALKGVQAGQLRDMCKQVGLEVVAIRRLRI
                     GKVSLAKMPVGAWRYLPVGERF"
     gene            9153..10010
                     /locus_tag="PK399_00060"
     CDS             9153..10010
                     /locus_tag="PK399_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007968468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HPW11553.1"
                     /translation="MKTPSGLQPLIDDGVIDAVMRPLKSGKEAAVYVVRAGDDIRCAK
                     VYKDMAQRSFQQRVQYQEGRKVRGSREARAIGKASKYGRKQQETAWKNTEVDALYQLR
                     DAGVRVPEPFGYFHGVLVMELVVDADGHSAPRLGEVELSALQARGFHRFLVHQVQKML
                     CIGLIHGDLSEYNVLVAPDGPVLIDFPQVVSAAGNNAARTMLLRDVNNLTASLGAWAP
                     ELLDTWYGEEMWALFEAGELRPDTELTGVFVHDESAVDVDGVRHAINDAREEALIRQQ
                     GREAADAMD"
     gene            10221..11321
                     /locus_tag="PK399_00065"
     CDS             10221..11321
                     /locus_tag="PK399_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPW11554.1"
                     /translation="MPSPQDPGDWSSPGHIHSLVLMAVTLAGLYLCYRMALPLLPALA
                     WALAFALLLVPVQRWLEPRLRSPGTAAGLLVLLGGVGVLFPAMLVADRLLDEVARGGG
                     AITAMLESGQWRRSISAYPLLVPAADWMEAQFDLPETANAMTAWLTTAVASLARESLL
                     QAIGMILTLYLLFYFLRDRRAILASIEALSPLARADTQRLFGVVDDTVHATVYGTLVI
                     AMVQGTLGGLMFWWLGLSAPLFWGVVMGLLAIVPVLGAFIVWIPAALFLLLDGSGGKA
                     LVLTLWGAIVVGGIDNLLYPMLVGRRMQMHTVLAFVSIVGGLALFGSAGLILGPVIFA
                     ITRLLLEIWSRRGERVAASRYTAEPMPPGDTP"
     gene            11318..11857
                     /locus_tag="PK399_00070"
     CDS             11318..11857
                     /locus_tag="PK399_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HPW11555.1"
                     /translation="MNTLDRPRYTPTLRRLHWLMAILVLAGYLLIEQRGLFPRGSAGR
                     SAMVQGHFWVGLLVFVLVWWRLLRRRADGAPAITPPLGRATALASRAFHVALYAFLVV
                     MPMLGLATAWSDGKAVLVPFTGIALPALLPENEALAHTLEDLHGSIGEAFYWVIGAHV
                     LAALWHHLVRRDDTLRRML"
     gene            11984..12991
                     /locus_tag="PK399_00075"
     CDS             11984..12991
                     /locus_tag="PK399_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPW11556.1"
                     /translation="MTAVLPSLGGIGAASAAARPRLLPVPLAKGDTIGLVSPSSATDD
                     SLNLQLAREAMEALGFTVKTGAHYDARRGHLAGTDAQRAGDLNAMFADKAVKAIVCTR
                     GGSGAARLLPLLDYAVIRRNPKVLLGYSDITALHCAIQAQAGLVTFHGPIGSGSWNPF
                     NVDQFRRVLLDRELVDYRNKVEAGDELVPRRNRTLTIRGGKARGELIGGNLTVLTALA
                     GSPYLPDFRGRILFLEDVSEAPYRVDRMFSTLKLMGALDQVAGVIFGECSDCNPGDGY
                     GSLTLEQILDDYLLPLGIPAYRGAMIGHLREQFIVPVGGKVELDADAGTFRLLEPVFQ
                     A"
     gene            13054..13485
                     /locus_tag="PK399_00080"
     CDS             13054..13485
                     /locus_tag="PK399_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11557.1"
                     /translation="MARKKAGDTTPSRKPDITVGKTPRPKLATGPLTVTAQGAILKDD
                     DRLIIVVRVLDDEGLPVTGLKKANFKLWQMAHLFDQSTSFFVVEIENIPGLEGMYHLV
                     QKTWSVVGNGTIPFFVRVEKGKLRSGAALTFIVKVRDGLDL"
     gene            13516..13737
                     /locus_tag="PK399_00085"
     CDS             13516..13737
                     /locus_tag="PK399_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11558.1"
                     /translation="MPIRPVDPASLPPWKRKSPPGKSRPLSALQIDAARERAAQAGRR
                     YPNLVDNMWAARHVPRGDADPAAGGDDEA"
     gene            13802..15205
                     /locus_tag="PK399_00090"
     CDS             13802..15205
                     /locus_tag="PK399_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW11559.1"
                     /translation="MLSLSFSRSLRVLACCLLLVLGACKPQQPAPAASADAATPAALP
                     AGAGPAKDEAWPAPDKVERDGPDAELVVQVGDLDNFGFGFPDGFDPFTGKSTPVHPFP
                     FQPGAKDARGTDRIMVVSGFKAADGDGYTSGTERPGNLPQALRVAFDLGGIEVTGAAL
                     QLFVDDFQAPVWGTRYQARINGVEVPLLATTLNALDQTGPIGKLVTVQLLPEQLSLLR
                     EGKLEVAIDDPINDVADGFAFDFARLLVNPKPWRYAGTIHGIAVDKATGEPLAGVLVS
                     ASNTRQATTSADGAFVLEGAPAGLAVVSGSHPDYQADTETSDLVAGERIEVRLELEPS
                     AKSGESLGEQLDKQGKVDLYGIYFDTDKATLKDESTAVLEQVLALLEARPQLPLIVGG
                     HTDADGSEAHNLTLSGRRAEAVVAWLVEHGVDASRLRAEGHGESRPVANNETGEGRAL
                     NRRVELRDASEAGGAAR"
     gene            complement(15227..>16636)
                     /locus_tag="PK399_00095"
     CDS             complement(15227..>16636)
                     /locus_tag="PK399_00095"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW11560.1"
                     /translation="AIDQFKQDTRYPAGTAVIVVKDGKIAYQGYFGFADIAAKTPVDA
                     ETVFYIASATKPFFALNALLAQDAGRLDTGTTLQAMFPDAHFSEVDPSAVTLRHLLTH
                     TSGIDNVALGWATAFSGVHDATSRQRLVLQSRPNAESPLGTFDYTNIGYNIASVWLDR
                     IDAAPWQAQLDARIFAPLRMRHTTALASQADARGWTVAKPYAFVSAYRTVPLYLRKTD
                     ATMHAAGGMLSTAPDLANFLIAQLANGKLDGTQRLPASVIHASQQRQATTDSKYLDFP
                     RDGYAWGWYTGDYKGRRMLHHFGGFAGFHAHLSFMPDANIGLVVLNNEDVLGARLTNL
                     IADCAYGIALDEPEIATKAKERFVQLAEDTATLEQAAIRQRDALRARPWRLSLPRETY
                     AGRYVHADLGEMSVTRQDDDALALRWGQLQAVASGYDKPDHVRVEFVPNSGQVLEFVV
                     DAGKVQGLVLDGMRFDKVR"
     assembly_gap    16637..16736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17058..17546
                     /locus_tag="PK399_00100"
     CDS             17058..17546
                     /locus_tag="PK399_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11561.1"
                     /translation="MPVVRHGFPWPLRLIVAGFGVFAMVVPAWELGRGLWPLSIATPV
                     FGIIVIGAASLGIAMLRAALAGESQVWTFPPNAVVIHHKAWRNAWIVRLTANNIAAIE
                     VRRLGASEGEDIWRVVIVPKASDSGLRMAAHDGGFETGDYTSAAYAEKVRRALLAHLG
                     MR"
     gene            complement(17557..18444)
                     /gene="yghX"
                     /locus_tag="PK399_00105"
     CDS             complement(17557..18444)
                     /gene="yghX"
                     /locus_tag="PK399_00105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HPW11562.1"
                     /translation="MTRMTASDFPPALLELYDGYVHGRISRRQFLDRASLLALGGMTA
                     VGALAALTPNYAWAQHVKFTDPDIVAEYITYPSPNGHGQVRAYRVRPAKADGKLAGVV
                     VVHENRGLNPYIEDVARRVAKAGFIALAPDGLSSLGGYPGNDDKGRELQKQVDPAKLM
                     NDFFAAIEFLAKDPDATGKVGITGFCYGGGVSNAAAVAYPELAAAVPFYGRQAKPEDV
                     PRIKAPLLLHFAETDPNVNATWPAYEAALKAAGTTYEAHVYPGTGHGFHNDSTPRYDE
                     AAAKLAWERTLSWFRRHLA"
     gene            18570..19475
                     /locus_tag="PK399_00110"
     CDS             18570..19475
                     /locus_tag="PK399_00110"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPW11563.1"
                     /translation="MLPLRLALVFLIIAGNTLLHVPPLVAMAFIKALLPSERLRVASN
                     PLLTGLAESWIGVNNWMWRTFTHTRVDLREDAGLRIDGHYLVLANHQSWVDILVLQKV
                     FNRRIPLMRFFLKRQLFWVPLLGLAWWALDFPFMGRYTKKQIAKNPDLARRDIDATRR
                     ACEKFRAIPVAIMNFVEGTRFTQAKHDAQGSPFRHLLKPKSGGVAFVLDAMGEGLHSI
                     VDVTIAYPAGTPSLLDLLADRIPEVRVKVRKRDIPADILRGDYQNDRAFRVQFQQWMN
                     GLWQDKDADMTRMLGQAASGDTGRD"
     gene            19531..19857
                     /locus_tag="PK399_00115"
     CDS             19531..19857
                     /locus_tag="PK399_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HPW11564.1"
                     /translation="MPVPSMSPSGRLFAAAALLEGLTWAGLLIGMWLKYGMQVTDTGV
                     WLFGRLHGGAFLFYVVVTVIAGVRLRWPSWALLLALLAAVPPLVTVPLEMWFRRKGLL
                     SDPATR"
     gene            complement(19867..20709)
                     /locus_tag="PK399_00120"
     CDS             complement(19867..20709)
                     /locus_tag="PK399_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPW11565.1"
                     /translation="MPRYHGPLLTRPLADALRAARDAGDTAWTGSFDLGRSRDTATLA
                     ETHWEWRGQRYPWADTLKDRTLYWWDGDEFAPISRYAGKLIKLVPTEWDVPTFEIDGI
                     KMLPTSKASPLDDARQKVALVQPAGKAVLDTCGGLGYFAACCLAAGAARIQSFEKNED
                     VLWLRTLNPWSPDPDAPEHGGRLQLTHADVSQAITTLPDAAFDAALHDPPRFGIAGEL
                     YSQVFYDQLARVLKRGGRLFHYTGSPNKLTSGRDVPREVAKRLEKAGFRAELALDGVL
                     AVRR"
     gene            20839..22902
                     /locus_tag="PK399_00125"
     CDS             20839..22902
                     /locus_tag="PK399_00125"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HPW11566.1"
                     /translation="MRLAPLTLSLGIALALTTVAAIAAQPGASNAQIGSFGVDLSARD
                     TTVKPGDDFNRYASGKWLDSYQLKDYETNFGSFNALRDRAEEQAHALIEELGKRNDLK
                     AGSNERKLRDFYASYMDRAGRDAAGIKPLQPVLDRYAAIDSKAKLVAAFGSDDIDGGN
                     APIGIGLGADRKDPDRYLASLGVGGLGLPDRDYYLNPDARFVKIREAYVAHIQRMLAF
                     GGINGADAKARAEAILALETALAKPMWERAKLRERDKTYNVVTFAELQKQYPGYDWAA
                     HLRAQGMQAPDRINVVTPDAVQPILDVIDATPLATWRDYLSFHAIDGNAGLLSKAIDD
                     ASFEFNGKVLGGQKAQRDDWKRAVALVGGRAGLGEALGELYVARYFKPESKAAMDKLV
                     ENLRAALRQNIAGIDWMGEATKAQAYRKLETFRPKIGYPSKWLDYSSVSVVPGNVLAN
                     VMAMRRYNRDDQNRRVGQKTDREEWGMTPQTVNAYYNSSFNEIVFPAGILQPPFFDVN
                     ADPAVNYGAIGAVIGHEMGHGFDDQGSKSDFAGIQRNWWTDEDRARFEQRTKALGAQY
                     DAYCPLPGQCVNGALTMGENIGDLGGISMAYTAYKLSLGGKPAPVIDGLTGDQRFFLS
                     WAQVWKGKYRDESLLNLIKTNPHSPVMYRANGPLRNFDPWYQAFDVKPGDAMYLPPEQ
                     RVRIW"
     gene            complement(22966..23121)
                     /locus_tag="PK399_00130"
     CDS             complement(22966..23121)
                     /locus_tag="PK399_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11567.1"
                     /translation="MAKPNYSFEKRQRELAKQKKKDEKDAKKRAEREAAKAGSAPADA
                     PATDAGE"
     gene            23234..23578
                     /locus_tag="PK399_00135"
     CDS             23234..23578
                     /locus_tag="PK399_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPW11568.1"
                     /translation="MIQRFDAGKRMSEATVHGGIVYLAGQVPEDTSADAEGQTRQVLA
                     EIDALLEKAGSDKRRILRAQIYLADIADFDAMNRAWDAWVVPGEAPARATVQAPLARA
                     GWKVEIVVTAAL"
     gene            23594..24514
                     /locus_tag="PK399_00140"
     CDS             23594..24514
                     /locus_tag="PK399_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HPW11569.1"
                     /translation="MSGQPYPIGVQGQPWGAAEKAAWRARQRKLRSHADEVMAKIDAL
                     RDRFDVEQYGELDYAPDGTYPLLAIRSRGWDDGLPTMLVTGGVHGYETSGVQGALQFV
                     ERDAAAYAGRANLLVVPCVSPWAYERINRWNPDALDPNRNFKPGSPARESAALLALVE
                     PLAGRIAMHIDLHETTDSDESEFRPALAARDGKAYEPGTIPDGFYLCADSARPEPAFQ
                     QAINDAVAQVTRIAPADPDGTIIGSPVVAPGVIEYDCRALGLCAGMTDARYVTTTEVY
                     PDSPRATPAQCNQAQAAAVVAAIDFALAHH"
     gene            24536..25675
                     /locus_tag="PK399_00145"
     CDS             24536..25675
                     /locus_tag="PK399_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HPW11570.1"
                     /translation="MDRRTFLQGSLVAAVAAAMPGEAEAQSAAAPAFPPIDARSVWLV
                     GDDAPPDPVAMTTRLAELARTHPEVRDRYQQKGAVEALERAFATLLGKEDCAFFATGT
                     LANHVAVRVLCGEHRRALVQQESHLYRDESDAAQRLSGINLVPLAAGRASPSLDEVRA
                     AIDASENGPYPLKIGAVSLESPVRRVDGEMLPLALVRDIASLARAHGARLHLDGARLL
                     LARPDLDIPAYAAPFDTVYVSLYKYLGAPFGAVLAGSKAEIAEARDLRHVFGGGLYQA
                     WMSAVLALDALPAFRQQIGQAHARVRKLIDALVASGRARERSAAEASNIFWLEMPQAL
                     AEAAFERGRQAGIRVGRWQDGRIPLHANTTILRRPLEDYLGLFGG"
     gene            complement(25688..26440)
                     /locus_tag="PK399_00150"
     CDS             complement(25688..26440)
                     /locus_tag="PK399_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW11571.1"
                     /translation="MSQFPAPRFATALAVEAYKLRRSLALLLAAVAPLLIAVFVFFNL
                     LRADKGAPWAISLQTSAAIWAFFMLPMSVTALTALVAHTEHGPRAWDHLRALPVPRWH
                     LYAGKAACVLGVVAAMSLWLALLTLLAVETAGVIKPAVAAIGAPDIAGYLALLGRIFL
                     AAWLLVAVQLWIALRHASFVPALAVGIGGTFFAVVATSAKVGAVLPWQIPVNQLATDP
                     LRVQFTLALGCIGGCAVFAAMLWRLSQREVLG"
     gene            complement(26457..27404)
                     /locus_tag="PK399_00155"
     CDS             complement(26457..27404)
                     /locus_tag="PK399_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW11572.1"
                     /translation="MPSAIETEGLTRRFGNRVAVDDVTMTVPERSIYGFLGRNGAGKT
                     TTIKLLLGLLRADAGTARIAGIDIATDRLRAARRIGALLEAQGFYPNLSGHENLDLTR
                     RLLGLPASEIDRVLDVAEMTAHGRRRVADYSLGMRQRLGLARAMLGAPPVLVLDEPTN
                     GLDPEGIADMRGFLRELPARSGATVLLSSHLLGEIEQTATHIGILSHGRLVLEGALAD
                     LKAGLAMEVAVETDTPERALLVAREHGFDVARDEAMRDRGVVVARFAAGEDVREATSA
                     LNRVLCIAGGVRIHALAPRQRSLETLYRNAALSAAPAAA"
     gene            27504..27995
                     /locus_tag="PK399_00160"
     CDS             27504..27995
                     /locus_tag="PK399_00160"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HPW11573.1"
                     /translation="MPITDRFRRQCRLLRAATLVVFVGLFLLLALGMIGLPWRMATDM
                     PAGWSTRALLSVVRFLPGLGYLWALWAVQRALGDLAAGRLFHPTVARAMRHIGIGVLA
                     GALFNVFAVTNLSRWIAGGHGGYLYFDLSGIVLGVVGAALVLLARLVDQARALQAELD
                     EIL"
     gene            27995..28234
                     /locus_tag="PK399_00165"
     CDS             27995..28234
                     /locus_tag="PK399_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW11574.1"
                     /translation="MPIRVTLDDMLARRGMRARELAATVGISETQLSLFRSGKVKGLR
                     FRTLAKLCFVLECTPGELLGYDRAVADLQQVDDDG"
     gene            complement(28259..28741)
                     /locus_tag="PK399_00170"
     CDS             complement(28259..28741)
                     /locus_tag="PK399_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPW11575.1"
                     /translation="MTDTRDLERKFWKAIDHDRTVMLGITGVAPRPMTALAEDDKAPL
                     WFFTASGTDLAESLRGSMGKQATATVTSKDHGLFATVSGKIVVDNDPAVIDRLWNPFI
                     AAWFEGGKDDPTLRLLRMDPEETHVWLNENSLLAGVKLMLGVDPKRAYEDKTGDIDIG
                     "
     gene            29057..29533
                     /locus_tag="PK399_00175"
     CDS             29057..29533
                     /locus_tag="PK399_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPW11576.1"
                     /translation="MRLLPFALSLCIGTAVAADPAAPATTPTFDAALAQRTGADERGM
                     RRYVLVILKTGPTRVPDGEARDAMFAGHMANIGRMAKDGTLALAGPFIQDPAGWRGLY
                     VFAVDDIEQAKALVATDPVIVQGEMVAEYHPWYGSAANMLVPEWHDRLVPPAKPAQ"
     gene            complement(29547..32585)
                     /locus_tag="PK399_00180"
     CDS             complement(29547..32585)
                     /locus_tag="PK399_00180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW11577.1"
                     /translation="MHLIRAVCLLLCLAVPAWAAPATTAPPPATASTAVRPLDLSDLG
                     APVFANFSARDGLPEQVLVDVRTDRQGFVWAASPQGVSRYDGRRWITEADPAMAHPVS
                     SLFVDRSGILWAAFRHHGLAHHDGQRWRVENATTGLPFDQVRRFAETVDARGQATLWA
                     ASWTGGLMLRRQGRWVADPGNATLPRSMTLSLANTTTIGGARRQWVGSGSHGLWYREE
                     GHSDWTQWRADDVNVAQVESLVTTTDADGEALWMSVFGVGLMRLDARGMQRWTQGKGD
                     LPTNQLYDIATTPLANGKRAIWVASRSGLLRVHDGRVQVFDRRHGLASDVIRGLNAWR
                     SPSGSEVLWLATEAGISRTVLDGSAWSTASLMGARSVGVFGVLVEPDGHGGERLWVGS
                     SDEGLGLYQAGRWQRFSADSGHLPSNGISMIRSLPDATGAGRTLWLGTWGGDLLRIDE
                     GPRFDRMATPWPKLNGQSVQDVLSRTVDGHDELWAGTRLSGAWRLRDGRWMEMRAPGI
                     DGQWRVSQMVEQRDRAGHAWLWAATDRGLARFDGRAWTLFDRSTGFPDDQLSGLRLYP
                     DARGRPILWMGSASVGIIRVDIGDATRPRVLPANLPPPPDPYTYGALRAPDGRVYVCS
                     NTGVQQLTADGKGGYTSRAFNRRDGMVHEECNGSAQFIDAHGRFWTGTLGGLAVYDPA
                     RATADTQPKPLRIVGMHVDGVPKPGAALRVPAGATTIDVEFALLSWDHEAESRFRTQL
                     VGLENAPTPWTAQPSRSFSALPPGDYTLRIEARDHAGNPSTPVDIPIHVAAHWWQQAW
                     AHAAAVLALLLLGYVAAWARTRVLRAQHDALEQRVAERTAELDAANARLLDLSYRDAL
                     TGLANRRRLLETLERTPAGSIAALVFFDVDHFKELNDRHGHPAGDEVLRRIVQAMSTC
                     TPSGALLARYGGEEFACLLPGMTTAGAGDVAERMRAAVEAMRVQIPDGMQDARVSISA
                     GVASRVLHTPEDRQRLLRDADIALYQAKDAGRNCVRISAG"
     gene            complement(32649..33221)
                     /locus_tag="PK399_00185"
     CDS             complement(32649..33221)
                     /locus_tag="PK399_00185"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPW11578.1"
                     /translation="MTRRYAAFALACLLAAPCFAALKPGTQAPLFKAPAYLAGKPFTF
                     DLAAALKKGPVVVYFFPAAFTSGCNVEAAAFSQAIDKFKAQGATVIGVTSGNTERLAE
                     FSSDTEKCAGKFAVAADTGAKIAKSYDATLAMKPDWSSRTSYVIARDGRIVHVHDAMN
                     PNLHVKETLDAVTALARATPAKAKAKASGG"
     gene            complement(33277..35181)
                     /locus_tag="PK399_00190"
     CDS             complement(33277..35181)
                     /locus_tag="PK399_00190"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HPW11579.1"
                     /translation="MNDMATSVVPAWGSALLHFLWQGALLGLLAGLGLTLLRNARPQA
                     RYALACAALSASLLLPALTLAWLLAAPRLTAAESLPLLVGANAGTANPNGLQHVLAMQ
                     ATTSMPWVVMLWGSGVATLLLRMAGGLWWVRRLQRRAWPEDPGHWQARADVLAGKLGL
                     PHNVRLRLFDGDAPLAIGWWRPMVLLPAALVARMPAGLLEALLAHELAHIRRHDYLVN
                     LLQAVVEALLFYHPVVWWLSRRIRHERELIADDLAADALGDRRSLALALSELDRMLGA
                     TAAGPIPRFAHAAHGGQLMSRIQHLLRPRQHPLAIGLSLPLLGLALAGAAMYAHAQST
                     APATAPTVDHTDTRHPPPPPAPPAPPAPPALPAPPAMPAPPPPPPPRADRVKKLHAGV
                     DAANGYAMVRKGDTGFRMSGSTDDVADIRRLRQGIDGDFLWVRRGGEAYVVRDAGVLA
                     SVDKAWGPVDALEVRMDALQARMQPHQQKLDALGSQMDRMAPVETPEMRAAGRQLEDL
                     ASRQQALAEKQARLAGKMAKADDAGRNALARDMDLLSEQMDVLSRQMDRHANVMDQQS
                     RAMQQQSGKMDAVARQMEAAAKPMEAIGRDMEALGNQIEREAGVADIRIRSLIDEAIA
                     SGLAQPVKRR"
     gene            complement(35174..35560)
                     /locus_tag="PK399_00195"
     CDS             complement(35174..35560)
                     /locus_tag="PK399_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPW11580.1"
                     /translation="MPMPRQPKPTPAELELLRTLWQLGPSTVKQVHEAQQRERPELAY
                     ANVLRLMQIMHGKGLLKRDERQRSHVYAVAQAQRSLQSRLLDDLIHKAFSGSGKALVM
                     AALRSDKVSDAERAEIQQFLEDTRDE"
     gene            35772..36920
                     /locus_tag="PK399_00200"
     CDS             35772..36920
                     /locus_tag="PK399_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW11581.1"
                     /translation="MRSTRRDLLKFGTLAAAAAALPSFATPGTATAPVARAAKPLDIL
                     ILGGTGFTGPFQVNYALSRGHKVTLFNRGKRPSPEWPGEVEQLHGDRNTGDLKSLAGR
                     KWDVCIDNPTSLPFWVRDAARVLKGNVGHYMFISTISVYADGSRHGIDEHSPLAVYKG
                     RDAMAETQESLRADMENLYGSLKALSEAEVRKHFGERTTIVRPGYIVGPRDETDRFTY
                     WPLRVAQGGEMLVPGDGQDPIQIIDGRDLGEWMIRLAEARAYGVFNAVGPDYTLTTDA
                     MLYGCQAVTANGVQLTHVTPKFIEDQKVELPIWVPRADNPYAGYGTVSNARALAAGMT
                     LRPLATTVADLLAWFHSLPADRQAKPRAGITREKEAELLAAWKAGKSG"
     gene            complement(36917..37951)
                     /locus_tag="PK399_00205"
     CDS             complement(36917..37951)
                     /locus_tag="PK399_00205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPW11582.1"
                     /translation="MKEGSELDRRLIVQLADSITDTHSLEQLVRPLLALLESATGLES
                     TYMTAIDETAGLQHVLYARNSSRMTIPEGLAVPWGDTLCKRALEEDRAYSDDVAERWG
                     DSAAARELGIATYASTPIHGPGGRLYGTLCAASDTSRPATDDAARVLRICSQLIGQQV
                     EREWLMQELRAANHALAMSALTDPLTLLPNRRAVMEELPRRIARRDREHGALLVAFID
                     LDGFKAINDVHGHEAGDNMLALIGEALTHTARAGDYCARLGGDEFVVLASVPAAEADA
                     ALGALHDRLQTATRGQFDLGGGITIDYDGPSIGTILVPEHQHDVDSVLALADTAMYAI
                     KRLRKAQRVR"
     gene            complement(37977..>38554)
                     /locus_tag="PK399_00210"
     CDS             complement(37977..>38554)
                     /locus_tag="PK399_00210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW11583.1"
                     /translation="ASSYRLVRKVQKQNLRLDELTRSDSLTGLQSRDHWQQQAGAMLA
                     WHAATGQPSSMLLVDVDWFKAINDRHGHAIGDDVLRGIAGVIRASLPEGSHAGRLGGD
                     EFAIVMPVDAGKARSIAECIRAGVATLDLPHLPMLRSSVSIGSAEPPTADADLRTWME
                     AADHALYRAKAAGRDRVSSTHSTDGTLAATH"
     assembly_gap    38555..38654
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<38655..39229)
                     /locus_tag="PK399_00215"
     CDS             complement(<38655..39229)
                     /locus_tag="PK399_00215"
                     /inference="COORDINATES: protein motif:HMM:NF017080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE2 domain-containing protein"
                     /protein_id="HPW11584.1"
                     /translation="MTRTNHRDDADGNGVPPHLRNLHRRTYPMRTLGMGLAVLPVLVV
                     LHELDAGWPAWAWTLFCGLAWPHVARLRAHYSRVPYRAELTNFMIDSAMAGSLAPIMH
                     FNLLPSAMLLTVATADKINAGVRGLWSRSLLGMLAAMLLSTLLAGWQVEIATSPPVVL
                     ACLPLMVIHTLAVSASSYRLVRKVQKQNLRL"
     gene            39339..39605
                     /locus_tag="PK399_00220"
     CDS             39339..39605
                     /locus_tag="PK399_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HPW11585.1"
                     /translation="MATGWAGDGAVQDQIDATVQDAIQRARRRLPSGPGRMHCEDCEA
                     LIPAARRKAMPGVRLCLACQELHDREEAGVAGYNRRGSKDSQLR"
     gene            complement(39682..40998)
                     /locus_tag="PK399_00225"
     CDS             complement(39682..40998)
                     /locus_tag="PK399_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11586.1"
                     /translation="MEKHQVEKIMTRHFTARRLAVSLMAALLAIGATAPAFALEQDKH
                     EDRDGRKQRKQQVDREESSREQPQRQEPQRQERQPPQVQERQADQPRPALQQGNGRWQ
                     PERRENWDQGSREREQQRRENLDVQARQREAGMQRQAEQREDMQRRIAEQRREQAGQR
                     EADERRLADQQRERQQQADRRADGQRRDNDERMARERAQQDRRTADQRDDRRDDRNEA
                     RDDRRDDRRDWRDDRRDDRNDRISREQQQRRIDDQRRQAMEWQRRESDRRSQLEQRNR
                     ELERQRRSHQYRYQQDYYRRWLAQQSRWNASRYDYYNDPYYSTAYNYRYSYGGRWYQT
                     NSYGRDLLQQAIRDGYREGWYAGQADRNDRWRFDYQGNYGYMDGTYGYSGYYVGRNEY
                     RYYFQQGFERGYRDGYYRRYQYGRYDNGVAVILPAILGAILNISRY"
     gene            complement(41142..41588)
                     /locus_tag="PK399_00230"
     CDS             complement(41142..41588)
                     /locus_tag="PK399_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW11587.1"
                     /translation="MRLLAIPLLSIAASLSMPGSAFAQQASEDVAARKPLEAYLQGHA
                     TGDPAYMREAFLPTAHIEGVRDGAFLSWTADEYIAGFKGAPPADEAQRKRWIDRVDVS
                     GNAAMARVTLDYPSGTFTDYFVLLKVDGEWKIANKVWTRREKPATP"
     gene            41715..43454
                     /locus_tag="PK399_00235"
     CDS             41715..43454
                     /locus_tag="PK399_00235"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPW11588.1"
                     /translation="MPLARCLLAVALASASVVVQAGQPALTRTTLASGLPLAPEQKAV
                     AFERAELSFKVDPARRWLEGDATLAFRAVRPIDKLVVDLDRNYAVDGVEVDGKAIDAA
                     HWRNPEGRMTVDLPKPLAVNADVLLRIRYSGHPHVAEKAPWDGGFVWATAPTGEPWVA
                     TAVQGEGCDLLWPCIDHPMGEPGEVVQHVTVPSPLVAAGNGIATGMEEQEGWRTYHWR
                     AKHPNTYAIALNIGPYELLQGEYRSRYGNTIPLRFWHLKGNTTKAEGLFAEFAPMLDF
                     FEEMIGPYPFADEKMGVVETPHLGMEHQTINAYGNGYKKDEYGYDWLLQHEFAHEWFG
                     NQLTHVDWEDFWLHEGFGSYMQPLYAQWLRGDMAYMAALMKQRAALANKYPVISGRGL
                     PENEVYAAGQGPGNDTYYKGSLMLHTLRGQIGDAAFFRAIRELVYGTATPQPGNFKPR
                     HASTSDFIAIVSRITGKDYRWFFDGYLRHAALPDLQATRDADGLSLRWKTGGEAPFPL
                     PVQVRVGDRVVDVAMTDGRGHVDLPAGATYTLDPHSRVLRELPYIAEFQKDAAERAKA
                     AAEKARSEAAKKP"
     gene            complement(43532..44785)
                     /locus_tag="PK399_00240"
     CDS             complement(43532..44785)
                     /locus_tag="PK399_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HPW11589.1"
                     /translation="MSPPSTYPARLAVIGGGPAGLMAAEVAHAAGIDVHVFEAKGSVG
                     RKFLIAGKGGLNLTHSEPRPTFDARYRERTAQIGTWLDDFDGDALRDWARGFGVDTYV
                     GSSGRVFPMDRKAAPLLRGWVRRLRDAGVHFHVQHRWLGWDDAGALRFATAGGETMFV
                     ADAVVLAMGGGSWPQLGSDGAWVRILTGRGIDIASLQPANCGFDIGWSAFLSSKHAGA
                     PLKPVVAHWRDAAGIEHALQGECVLTETGIEGSLIYAIAADLRDAIARDGHALLHLDL
                     VPGRTPERLQADLAKPRHGRSLGEHLRRQAGIDGAKLALLHEALDKPALQDMARIVAT
                     LKRFPLRLLRPRPMAEAISSAGGVRLEAMDDALMLRDAPGVFCAGEMLDWEAPTGGYL
                     LTACFASGLRAGRGATRWLREMGAG"
     gene            44795..46351
                     /locus_tag="PK399_00245"
     CDS             44795..46351
                     /locus_tag="PK399_00245"
                     /inference="COORDINATES: protein motif:HMM:NF017142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11590.1"
                     /translation="MPRLQPPAHPPYPLRNPKWMPSMSRLLPALLLFLLLPLTGVAAD
                     WDGKPNRYRVRVDAQSTRAVVEADLWQQSDTLSMYNVMEVPGLPNGQASLVDGMRVTD
                     AKGAALALEDLGAGDYAIAGGRRIRLSYSVRLEHDRYPWPAGMEEVSYRTDEGVMVTG
                     AALFFADGDAPVKAPIEVDFELPQGWHARTPWTRIGGGDRFRVDSRRELVSNALFLGT
                     AQVETFDAGGVELTLVLGQRYRKSAPLFVDLLRTQLASYADLFGGPPLSKRYLVIINE
                     HESGDGGAFASSFSQFVQGDADARNRVIWGYVMAHELLHFWNGLSIVPADHREEWFKE
                     GVTDYLTIVTLARNGWLDENLLFKRLENVPRRYLIARYAQHLQMSVRDAGKDKQPNRQ
                     LVYGGGSLAALALDVALRERSGDKVGLPEFMRALHAEFAKPGKAYALADLERIAGSLT
                     GSDFSGFFAEAVGNTSYFDIRPSYAAVGLRMDSFVEEMFISREPAATPVQQARFRAVF
                     GTPGAPCPRQ"
     gene            46370..47161
                     /locus_tag="PK399_00250"
     CDS             46370..47161
                     /locus_tag="PK399_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylargininase"
                     /protein_id="HPW11591.1"
                     /translation="MWTAITREVSPALADCQLSFVERDAIDVERARAQHAAYCAALEG
                     LGCRVLELPAEAAWPDSVFVEDVALAFDELAVATRPGAESRRDEGSAVLELLGSVRPL
                     SRIEAPGTLDGGDVLRIGKRVFVGISARSNAAGHEQLRALLAPHGYAVASVATRGCLH
                     LKSAVTQVGEATVLVNPAWVDVATFADYAIIEVDPAEQHAANAVRVGDALLYPEGFPR
                     TLARLRSAGIEVVTVDVSELQKAEGAVTCCSILLRHAAGHREVAA"
     gene            47158..47922
                     /locus_tag="PK399_00255"
     CDS             47158..47922
                     /locus_tag="PK399_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HPW11592.1"
                     /translation="MILNIAAYLFVAIDDADALAVRLRERAEADALRGSVLVTPEGVN
                     LFLAGDADVLRRFLAWLREDPRFASLQAKESWSETVPFARLKVKRKEEVIAFRREHAS
                     PLDTGERAPAVAPATLARWIEQGHDDDGKRLVLLDTRNREEFGFGSFAGALTLPIDNF
                     TDLPDALAPLRGALADATVVSFCTGGIRCEKAALWLRHDGMDNLLQLDGGILGYFEAV
                     GGVGYDGRCFVFDERVALDPHLQPLVDGMATKDAAA"
     gene            47919..48071
                     /locus_tag="PK399_00260"
     CDS             47919..48071
                     /locus_tag="PK399_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11593.1"
                     /translation="MSWIGFVVLIVAIYAAIKVVGALFKIALWVAILGFAWWYFGPRI
                     GIPFPF"
     gene            complement(48117..49310)
                     /locus_tag="PK399_00265"
     CDS             complement(48117..49310)
                     /locus_tag="PK399_00265"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW11594.1"
                     /translation="MSERSYDALVVGAGAAGLMAAITAGKRGLRVLVVDHANKVGKKI
                     LMSGGGRCNFTNTGTTPANYLSANPHFCKSALARYTPWQFIDLVERHRIAYHEKELGQ
                     LFCDESSKLIVRMLLDECTAGGVEVRTQCTIERVEHLDDGGFRLRTSQGSFAAPAVIV
                     ASGGLSIPSMGASGFGYALAKQFGHEVLPTRAGLVPLTLTGKHAERLQDLAGVSLPVE
                     ARCNGASFRNFMLVTHRGISGPSILQISSYWQPGDDLRLDLLPGTDADALLRNWQASR
                     RDAELKTLLGEVLPKRFAQRLCEHWITSKPLRQYNAPELRGIADLLSSWPLVASGTEG
                     YRTAEVTLGGVDTDGLSSSTMMSKHVPGLYFIGEVVDVTGWLGGYNFQWAWASGHAAG
                     QALCG"
     gene            complement(49307..50062)
                     /locus_tag="PK399_00270"
     CDS             complement(49307..50062)
                     /locus_tag="PK399_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPW11595.1"
                     /translation="MPRLLVFQHVAAEPLGTLDPLIRKRGHRIRFKNFERHPDAQLNV
                     DRYRGLVVLGGPMNVEDHPRRPHLRNEMIAIERMLEQGKPVLGICLGAQLLAHVLGAP
                     VRRNEVPEIGWYPLRKTTEGTSDPVLAPLQADTPVFQWHGCRFDIPEGAVHLARSPQC
                     EQQAFRHGDNAYGFQFHLEMDERLVERWLANPAYREELADSGLPHDADAIRAQTRLHI
                     AAMQQQADAVFGNFLDLVGRPQRRFTLPSREWA"
     gene            complement(50129..51937)
                     /locus_tag="PK399_00275"
     CDS             complement(50129..51937)
                     /locus_tag="PK399_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW11596.1"
                     /translation="MSAETTPDIKFTDLGLPETLLQALRTVGYESPSPIQAATIPPLL
                     AGRDMIGQAQTGTGKTAAFALPALAKLDAAASKPQVLVLAPTRELAIQVAEAFQKYAT
                     HIPGFHVLPIYGGQSYQPQLSALKRGVHVVVGTPGRVIDHLERGSLDLSALRMLVLDE
                     ADEMLRMGFIDDVEAVLKKTPETRQVALFSATMPAQIKRIAQTYLKDPVEIAIKSLTS
                     TAENIRQRFWMVSGINKLDALTRIMEAEPFEAMIVFARTKLGTDELAEKLAARGFSAA
                     AINGDVDQKMRERTIQRLKDGQIDILVATDVAARGLDVDRISHVLNYDIPYDTESYVH
                     RIGRTGRAGRKGEAILFVAPRERGMLGAIERATRQKIEQMNLPSVDAVNERRVAKFLD
                     KIEGALGSADLSVFRDLVERYEREKNVPAVEIAAALAQLVQGKTPLLLAKPAAPERAF
                     EVREPRERSERPERGERPVRERAPREHAPREHGAPEVGMKTYRIEVGYQHGVQPGNIV
                     GAIANEADLEARFIGRIDIRDDFTLVDLPDGMPADLLAHMQTVRVASRPLRMRPAESA
                     DIDAPKAKRSFGPPRGDRPGGGPRKPGGFKPRGPRF"
     gene            52047..53627
                     /locus_tag="PK399_00280"
     CDS             52047..53627
                     /locus_tag="PK399_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HPW11597.1"
                     /translation="MHFPTFDNRLLRALPGDPETRVFPRQVQAALWSSVEPTPVDAPR
                     LLAHSRDMAAELGFSEADVASAEFAGVFGGNALLPGMQPFATNYGGHQFGHWAGQLGD
                     GRAISLGEAVLADGRRRELQLKGAGPTPYSRSADGRAVLRSSIREFLCSEAMHHLGIP
                     TTRALSLVATGEGVVRDMFYDGRPEVEAGAIVCRVAPSFLRFGHFELPAMRGDIDLLR
                     KLADFCIARDFPHLQGQGALRQAQGERIYADWFAEVCERTARLVAGWMRVGFVHGVLN
                     TDNMSILGLTIDYGPYGWIDAYDPDWTPNTTDAAGRRYRFGWQPRVAYWNLGQLARAL
                     SPLFADPAPLHAGLERFATAYTAADRDNTARKLGLAECRDEDIDLLRDLHGLMQSHEV
                     DMTLWFRALADLDPARPSLTPLEDAFYDAGKRVAGEAAFNDWLARYAARLNEDPLVGD
                     ERRARMHAANPNYVLRNWLAQEAIDRADAGDMAGIGDLLEVMRRPYDDQPGRERFAGK
                     RPDWAKDKAGCSMLSCSS"
     gene            53870..55747
                     /locus_tag="PK399_00285"
     CDS             53870..55747
                     /locus_tag="PK399_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW11598.1"
                     /translation="MPLITLQGLDYSVGGPLLLEHVDLAIEAGERIALIGRNGAGKST
                     LMKLLAGELKPDDGEVRVEGGRRITRLEQEVPLDAAGSVFDVVAAGLGDVGAQLAHYH
                     HLIHAEPIDMDALADVQAKIESANGWSLDQRVIETLERLGLDGDADFAALSGGMKRRV
                     LLARALVSQPDLLLLDEPTNHLDITAIDWLEGFLKQWSGALVFVTHDRRFLRALATRI
                     VEIDRGQVTSWPGDWDNYQRRKEERLNAEAQENARFDKLLAQEEVWIRQGIKARRTRD
                     EGRVRRLKAMREDRAQRRAQTGNVKMATAQAGESGKKVIEAKGVSFAHGDNVLVRDFS
                     TVIMRGDRIGLVGPNGSGKTTLLKLLLGSLQPQAGEIRSGTQLQIAYFDQYRATLRED
                     WNAIENVAEGREFVEVGGKQKHIIGYLQDFLFTPERARAPITRLSGGERNRLLLARLF
                     AQPSNLLVMDEPTNDLDVETLELLEELLGDYPGTLLLVSHDRDFLDNVVTSTIVMEGD
                     GRIGEFVGGYSDWLRQAASSAERQAQAPARSTPPAAAPAVSTQPKAKRSFKELKELEQ
                     LPLRIEALETQLAAMTARMAEPGYYQRDAAQVTADNAAMAASQAELDAAYLRWAALEG
                     G"
     gene            55888..56244
                     /locus_tag="PK399_00290"
     CDS             55888..56244
                     /locus_tag="PK399_00290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPW11599.1"
                     /translation="MLAEYLPTLLFLIVATGIGIVLIVVGNVLGPKRPSADKLDPYEC
                     GFSAFENARMPFDVRYYLIAILFIVFDLEIAFVFPWALVFRELGVFGLVEMGVFLGLL
                     VIGFVYVWKRGALEWE"
     gene            56241..56831
                     /locus_tag="PK399_00295"
     CDS             56241..56831
                     /locus_tag="PK399_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011259750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPW11600.1"
                     /translation="MSNIVQKVLEIASNPLPEGRLDDILRPEGDNPLLQNGFVTTSMD
                     ALWNWARTGSMWPMTFGLACCAVEMMHAGAARLDLDRYGVVFRPSPRQSDVMIVAGTL
                     CNKMAPALRKVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCP
                     PTAEALVYGILQLQKKIRRMEQNPFSGAAKPEGARA"
     gene            56828..57439
                     /locus_tag="PK399_00300"
                     /pseudo
     CDS             56828..57439
                     /locus_tag="PK399_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927494.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
BASE COUNT         8812 a        18993 c        19960 g         9474 t
ORIGIN      
        1 ttgtcgaggt cggtgaaatc gaagaccttg ccgggctgca ggtagctggc cggcacgaag
       61 ttcttcacga acaggatcgg gtcgacgatc ttcagcgtgt acgagccgcg ggtgaccgcg
      121 ccgacctgcg tattcaggaa gccatcgtcc cagtagatct cggactgggt gccgaagcgg
      181 ttgtccggca gttccttcag cgagacgtag aacgccgcct gctgcgaacc gggctggccg
      241 ccgaacttga agcgctccca gctctgcatg atcagcgggc tgacgatgcc gtccccggcg
      301 aagatcgact tcgagttgat gtcgtcggag cgccattcgt agccgccggg ctcggcgacg
      361 aagccggtga tcttgccgtc ctggaacagc agcaggccat agccttccgg caccacgatc
      421 ttcgagccgt tggtgatgat gttggaggag gcgctggtgt tggagccgcg ccccgcattg
      481 gtgccctgcg gcactgcggc gaacagggcc gccgtggacg gcagtcctgc cggcaccgtg
      541 tagaagtcct tccactggtc gccgagcatg ccgccgaccg cacccgccac cgcttgaatc
      601 agacccatga ccgctctccg cgattgatgt ccgggcacct tgcccggctt gctccccgaa
      661 ctatacgttc ccgcgcggaa acaggggcca tcgccacggc ccgtcgcatg acgaagcgtg
      721 gcgggctgtg ctgcaatccc gcacatgccc gatcccaacc tcgacgcgct gcgccgctac
      781 gccgtcgccc gcacgctgtt caagccgacc acgctggcgc gcgcgatcca gcgcctgggc
      841 ttcctccagg ccgatccgat ccggtcgccg gcgcgtgccc aggacctcac cctgcgcctt
      901 cgggtcacgg gttaccgcgc cggcgacctg gaacgacgct accctcggct ggcggtggaa
      961 gaagactgcc tggtcaatta cggcttcctg ccgcgcgaac acctggcgct gatgcacccg
     1021 cgcgaaccgc gcaaggcctg ggatgccgcg acccggcgtc gcgccgacga ggtactcgcg
     1081 ttcgtgcgcg agcgcggacc cgtgcatccg cgcgaggtcg accagcattt cgcccacggc
     1141 cgcgtgcgca attactgggg cggttccagc aatgccagca cgcagttgct ggatggcttg
     1201 cattaccgcg gcctgctgcg cgtgcagcgc cgcgatgggg gcgtccgcgt gtatgccgcg
     1261 acggcgcatg cgccgaccga cgacagccgc gagggacgcg cgcgccgtgc tgccgcgctg
     1321 gtcgacctgg tcgtgcgcaa gtacgccccg ttgccggcgg ccagcctgac ctatctcgcc
     1381 cgcctgttgg gctacggtgc gccgcacctg gcgagcgaga tccgcgatgc ccttgcgcag
     1441 gcgcgtgcgc gattgccgag ctgccgcatc gacggcacca cctggtattg gtcggaaggc
     1501 gaggatcctg ctgcgcgcag gcatgcgatc gacgacacgt tgcgcctgct ggcgccgttc
     1561 gatccggtgg tctgggatcg ccgccgtttc gaactgttct ggggctggac ctacaagttc
     1621 gaggcctaca cgccggcggc gaaacgcagc ttcggctact acgcgctgcc gatgctctgg
     1681 caggggcagg tgccgggttg ggccaacatc agcctgcgcg gcggccgcgt tgaagcggag
     1741 gtcggctacg tcggcggcgc accaaggggt cgcgcgttcc gcgctgcact ggatgacgag
     1801 ctgcagcgga tgaccgagtt cctggcctga accacgcgcg gcgcgggcag gtcagcgctg
     1861 gtcgagttcg tggtggatca gcaccacgca ctgcgacgcg aacagcatct tcgaacccag
     1921 ggtgagcttc ttcgccccga ggcgttccag gctggggaag atcttcttgg gcatgggaat
     1981 gtggtcggtc agcaggaagc aggggttccc ggcgaaggcc tgttcgcgga tcgaggtgcc
     2041 cttgggatcc atcacgaaca actggtggtc ctgcgccagt tcctgcacca gccgctcgaa
     2101 gctgagcgta cgcacgtcca cgccgcattc gaccggtcgc gcttcgtcct tgcccatgcc
     2161 gcgcgatgcg tccagcgccc tggccacctt gcccagcagg gagcgttcgt cgaagccgcc
     2221 gatgtcgtgc atggcgttgg cctcgaagcg gatcgtccgc gagaaatcct gcgtgctttc
     2281 cagcaccagg tagaccacca catccgcacg gtgcgactgt gccacgaaga tcgcgttcat
     2341 cagcgtgtgc gccaggatct cggtatgcgc ctcctggccg acggcatcca ggagtttgcg
     2401 gctgtcggtg ggtgccgcgc gcgcgcgcag tacgaaggtt cgcatgtgtg tcccggtcga
     2461 aaggcccgcc accaatgcgc ggccggcacg cgcgcattgt ccgtgcaaat gcaggttcac
     2521 gacatccccg tggatcccgc gggcatgcaa ccagcggtgg cgttgtcacc gcgttttcat
     2581 tcgatcggct agggtcgcgc ttccttcgct cgcatcggag tccgcatgtc cttccttttc
     2641 catcgtggcc tgccggccgc catcgctgtc atggcgctcg ccagcgtggg ttgcgcgagc
     2701 cagcgctcga ccgccgcgct cgcgccgggc agccaggcct cgctggaagg caccatcgcg
     2761 tcgatcgaca cccagccgtg gacctacgac ggcaatgcgg tcgtcctgct ggacaccacc
     2821 acccacggac aggtcccggt gcaactgccg gcgcgctgga acctgtgcaa ggcggcgtcg
     2881 gtcgatgtcg ccgcgatgcg caccggccag cgcgtgcgtg ccatcggtac cgtcatcgac
     2941 gaccgcgcgc ttgtggtttg cgagcgcacc gaacatcgct tgcagccact ggactgagct
     3001 cgacctcgac ggccggctat gatccgcgca tggccaagct cctcctcaac ctgcgccacg
     3061 tccccgacga cgaggccgac gacgtgcgcg agatgctcga tgcgaacggc atcgcctttt
     3121 acgagacaaa gccgggtcca tggcggattt cggtgggcgg catctggatc agccaggatg
     3181 ccgcgatcgt ggaggccaag cgcttgatgg ccgagtacca gctggcgcgc ggcgcgcgtg
     3241 cccgtgcgga atacgccgcc gccgtgcgcg atggcaccgc ggagacgttc tggtcacagt
     3301 tgcgggaaga accgctgcgc gtgttgctca ccgtggtggc gatcgtcctg ctgctggggt
     3361 tggtcgcatt gccggtcgtg ctgttgcggg cctgatcgat gcggacgggc gtgttcggtc
     3421 ccttcggcct ggtcctgctg ctcgcgctga cgggcggttg cagcggaggg gcggtgcagg
     3481 cggacatcgc ccgcccgtcg gtgtcgccac cccgcatcct ggtgttcacc cgcacgacgg
     3541 gctggcgaca cgattcgatc ccggtcgcga tcgaaacgct gcgcgatctc ggcgaagagg
     3601 ccgggttgca ggtcgagcac agcgaagacc cgatgcagtt cagtgccgcc acgttggcgc
     3661 gctatcgtgc cgtggtcttc gccaacacca cgctggacgt gctggatgac gcgcagcagc
     3721 gggcgttcga gcggttcgtc ggcgcgggtg gcggctatgt cggcatccat tccgcggcgg
     3781 ataccgaata cgactggccg tggtacggcg agctggtggg tgcatggttc gacagccatc
     3841 cacccggctt gcaggcctcg ccggtgcgct tcgaggcgcg gcacgcgcta cctggcgagc
     3901 cggactggca agtgcgcgac gagctctaca actaccgacg caatccacgc gcatgggtcg
     3961 atgtcatcgc caccgtcgac gaatccgcct atgccggcgg caagatgggc accgaccatc
     4021 ccatcgcctg gtgccaccag cgcatgggcg gccgcgcctg gtacaccggg ctgggccatg
     4081 acatcccgct gtacgcggat gcggggttcc gcacacacct gctgcgcggc ttgcgctacg
     4141 caacgcgaca gtcggacgac tgctgaaggc gcactcggta gactggccgc cactcccgcg
     4201 cgcaagcctg gcacatgatc aacaacgaca tcctgcgcag catccgttac atgctggacc
     4261 tcagcgacaa caaggtcatc gagatcatcc gcctggcgga tcccgaattc gcgatcgaga
     4321 aggacgagtt gcggggctac ctgttgaagg aagacgagcc gggccacgtc gcctgcggcg
     4381 atcgggtgat ggcgcatttc ctcgatgggc tggtcatcta ccggcgtggg cgcgacgaat
     4441 ccctgccgcc gcgaccggtc gagaagcgga tcaacaacaa cgtggtgctg aagaagctgc
     4501 gggtcgcctt ccagttcacc gacgtggaca tgcaccgggc cttcgccgac gccgggttcc
     4561 cggtgtccaa gccggaactg tcggcgctgt tccggcaacc ggggcacaag aacttccgcc
     4621 agtgcggcga ccagctgctg cgcaatttcc tcaagggact gaccctgcgc atgcgcgggg
     4681 cgtgagcgcc gggaggttga cggcgcaagg cgtccgcccg ggcacggata gggcagcggg
     4741 cgccgcggcg gtgccggtct ggcggggtac cttgccgcca aggacgacag gttccacctg
     4801 aatacggtcg tgggtgcatc cggatcgcgt gcgatcccgt atgcccatgg gtcggcgccc
     4861 gcgccggcca acaaccctca ccacgaccag cagtcaggag acttccgatg aagcgtgccc
     4921 ttgtcccggt actcgtcctt gccagcctcg cccccatggc cgcaatggcg caggatggcc
     4981 ccagtttttc cctcatcgcc gggcgcgacg tgtccgtcag cggcgatgtg cacaagggcg
     5041 cgatttcgcc catcgccgac ctcggtccac tcaacccggc actggccggg gtttcggcgg
     5101 aattgcggat cgaatcgcgt tcctacgacg acatctacgg gaatgccaat accttcggcg
     5161 tggaaatgag ctggccgatg gccggcggag aagtcttcgg ccagttgcgc agcagtcgct
     5221 ccagcgacgg cgaggtgcag gtcggcggcg ccttcgtgcc ggccctgaac accacgctgc
     5281 cggtgtacgg tcgtttcgac ggttacagga gccttgcgct ggagggcggc taccgccact
     5341 acctgtccac cggcaagctg cgtccctacg tggcaggccg actgggtgcg acgcgcgtcg
     5401 acgacatcaa cgccacgttc tcgatccccg atgccaacat cctcatcagc gacgtgccgt
     5461 tcttcgacgg cggatggaac ctgagcgggg gtggcgacct gggcgtgatg tgggcgctct
     5521 ccgatgcggc ctcgctgggc gtcgaggtcg gtgcgcgcta ccacggtgat ctggacggca
     5581 acgacagtgc catcggcggc ctcggcctgg cgtcgatcaa caacaccggg tcgcgcacgt
     5641 cgttcccggt gagcctccgc ttccagatgc gcttctgacc gacccatccg gggcccgcca
     5701 accgccgggc cccggatgca ggcgcgatcc atcgcggcgt tgccgcgact tccccgtgtg
     5761 ccggcaggca accatgctgc tgccttgcgc tcaaggcgcg catgccagtc cttgagatca
     5821 ctcgccccac gacgggaacg ccgcagaccc tgaccatcgg cacatgtttt ggtgtgttac
     5881 ttcactaaca taccagctca tctaagtcgg gatgtgcggg catggcgctg caattggcac
     5941 tggaatcggt gggatatcgc tatcgcggtg gccacgaagc ggtcgcgggg gtgggcttga
     6001 cgatggcccc cggcatcgtc ggcctgctgg ggccgaatgg tgccggcaag tcgaccctga
     6061 tgcgggtgct gtccaccctg gccaagccca gcagcggacg ggtgctgtgg cggggcgagg
     6121 acatcgcccg caagccggat gtattgcggc gcgagctggg ctatctgccg caggacttcg
     6181 gcgtgtacga ggcgctgtcg gcgcgcgagt tcctggcctt cctgtcggcg gtgaagggct
     6241 tgccatcgcg gacggtggcc gcccgcgtgg acgcatgcct ggagatggtc gggctgggcg
     6301 acgtggcgga tcgacgcctc ggcggctatt ccggtggcat gcgccagcgc gtcggcatcg
     6361 cccaggccct gctcaacgac ccgaagctgc tgatcgtcga cgaacccacg gtgggcctgg
     6421 atccggagga acgcgtgcgc ttccgccacc tgctcgcgga gctggccggc gaacgcctgg
     6481 tgatcctctc cacccacatc gtgtccgaca tcgaagccag tgccggcatg ctggcgatca
     6541 tgagtggcgg tcgcctgcgt ttccacggca ccccggaaac gctgatggcc caggccgagg
     6601 gccatgtctg gcaatggacg ctgcctgccg aacagctggc agaggcacgc gcgaggttgc
     6661 ggatcagccg cgcgcaacgc cgcgcgcatg gcgtggaggt gcgcgtggtc ggcgatgacg
     6721 ccccggcagc ggatgcaatc ccggtcgcgc cggatctcga agacgcctat ctgtggctcc
     6781 tgcatcgcag cggcgcgcaa tcgtgagcgg tgcgcgcgcc gtcgctgcca tcctggccac
     6841 ggacctgcgc cagcgcctgc gcacgccgcg cttctggttg ctgatcgcgg ggctggccgg
     6901 attgatgtgg tggtgcttcc ccgcgatcga ccgcgactac ctgacggtct cggtgggcga
     6961 gggcatgcgc ggacggtatt ccagcgcatg gatcgggatg gtgtccgcgc tgatgtacag
     7021 ctcgacgctg tcggtggccg ggttctacct ggtgcgtggc accgtggtgc gcgacctcga
     7081 aacgcgcacc tggcaattgc tggtggccac cacgatgagc cgcggcgctt acctgctggc
     7141 gaagtggctg agccacatgg tggtgttcgc gttgctgatc ggtgccgggc tcgccgtggg
     7201 gcttgtcgca cagatgttgc gcgcggaaga cctgcgcatc gacctgctgg aactggtcaa
     7261 gccggtgctg gtgctgacga tgccgtcgct ggcactgacc gctgcgctgg cggtgtggtt
     7321 cgacctggtg ccgtggttgc gtcgcagcgc gggcaacctg ctgttcttcg tggtgtgggt
     7381 gatgctgctg acgttcggcg tgtcgcaggc cgaacaggcg cccggcgcgg cgctgccctg
     7441 gcccggcgac ccgcatggcc tgctggtggc cgaatacgac ctgtcgcatg cgtggccaac
     7501 ggtggatgcc ggcaaggagc ttggcctgag cgtcggctcg caggcgctgg agggcaaggc
     7561 gccggtcttg ctggactgga cgcattggca gctcagcgcc gccaccctgc gtgcgcgtgg
     7621 tgcgtggctt ggcctagcgc tcgtgttgct ggtgctggcc gtgccgttcc tcgaccggtg
     7681 cgcggcgcac gtcggcaatc cgcgcaaggc gctgggcgat ggcgcacgcc tgcgctggct
     7741 ggatgcggcg ctctcgccct tgcaacgcgg caccttcgga accttgctgg ccgcggaagt
     7801 ccgcctggcg ttgcgtcccc gcaaggcgtg gtggtggctg gcgttgctgg cggcgatggg
     7861 cgtgcaggcc ttcgcgccgg agaaggcgat ggcggtcgcg gtgatcgtgg catgggtgct
     7921 ggcgatggac gtgttcgcac ggctcgtgtt gcgcgaacac gagacccgca ccgcagccct
     7981 ggtgttcacg gcgccgggca tgcgcacgcg gctgctgcag gtccgcgcgc tcgtggccgt
     8041 gggcctggcc tggctgctgg tgctgccggc gatcctgcgc ctggccgtgg tcaatccgca
     8101 ggccgcgctg tccgcgctgg tcgttggcgc ttcgttggct gcatggggga tggcgaccgg
     8161 cgtgctgttc cgcaacggcc gcatgttcga gctggcgttg ctggcgctcg cctacatcgg
     8221 cgtgcagggc ggtttgctgt tgaacgtcgc ggtggcgcca atggacacat tgcgctggca
     8281 tctgctcgga ttgccgctgg cactggcgat cctggcaatc ggctggccac ggatggaaac
     8341 gcgctgagcg gagacctgcg ttcggcggcg cctcagaagc gctcgccgac cggcaggtaa
     8401 cgccaggcgc caaccggcat cttcgccagc gagaccttgc cgatgcgcag gcggcggatc
     8461 gccacgacct ccagtccgac ctgcttgcac atgtcgcgca actgcccagc ctgcacgccc
     8521 ttcagggcga agcgcaggcg gatctcgttc tgccagctga ccttgcacgg cggcagcgcg
     8581 cggccgttgt aatgcaggcc atggttgagc ttgcgcagtc cgtacggagc gatctcgccg
     8641 ctgacctcga ccacgaattc ctgttcgatc tcgtcgccgt cctcggtcag ccggcgccac
     8701 acgcggccgt cctgggtcag caccatcagg ccgctggcct cggcatccag tggcaccagc
     8761 ggagtgaggc gatggaagtg ccgatcgagc atgcgcaccc cagacggatc ctcggcccag
     8821 cgcgtgtccg gctgcaccag cgccgctgcg gccttgcgac cactgatggt gtcgtagccg
     8881 accggcttgt gcagcaggat cgtcgccggc tccacggttt ccagttgcgc ggcgggatcg
     8941 atcgacacgc tttcgccggt caccggatag gcaggatcct cgaccacgcg gccgtccacc
     9001 gatacccagc cgcccttgat gtattgctcg gcctcgatgc gcgagcatcg ggccaggtcg
     9061 gccacgcatt tggccagtcg cactggatcg gacatgcatc gacaccacgg aatcgggaca
     9121 ggcagtgtag cgatcaggca cgctatcgcc ccatgaaaac cccatcgggc ctgcagcccc
     9181 tgatcgacga cggcgtgatc gatgccgtga tgcgcccgct caagagcggc aaggaagccg
     9241 ccgtgtacgt ggtgcgtgcc ggcgacgaca tccgctgcgc caaggtctac aaggacatgg
     9301 cccagcgcag tttccagcag cgcgtgcaat accaggaagg ccgcaaggtg cgcggcagcc
     9361 gcgaggcgcg ggcgatcggc aaggccagca aatatggccg caagcagcag gagaccgcct
     9421 ggaagaacac cgaggtcgat gcgctgtacc agctgcgcga cgccggcgtg cgcgtgccgg
     9481 agccgttcgg gtatttccac ggcgtgctgg tgatggagct ggtggtcgat gcggacggcc
     9541 attccgcgcc gcggctgggc gaagtggaac tgtcggcgct gcaggcgcgc gggttccacc
     9601 gcttcctggt ccaccaggtg cagaagatgc tgtgcatcgg cctgatccac ggcgacctgt
     9661 cggaatacaa cgtgctggtc gcgccggacg gcccggtcct catcgatttc ccgcaggtgg
     9721 tgagcgccgc cggcaacaat gccgcccgca cgatgctgct gcgcgacgtc aataacctga
     9781 ccgccagcct tggcgcgtgg gcgcccgagc tgctcgacac ttggtacggc gaggagatgt
     9841 gggcgttgtt cgaggcaggc gagttgcgcc cggacaccga actgaccggc gtgttcgtgc
     9901 acgacgaatc cgcggtcgac gtggatggcg tgcgccatgc gatcaacgat gcgcgcgagg
     9961 aagcgctgat ccgccagcag ggccgcgagg ccgcagacgc gatggattaa gtggtttcgc
    10021 aggcgcacgg ggcctcgccg tcttgaccgg gatcaagaca tgcgccggcc gaaggcgtag
    10081 gttggggcga tgccaagcgc cacgccacgc catgtccccg tcgatagcgc aggtacgcaa
    10141 gctgcgcaac gacgtcgcgg gcgtggattg cgcgcacgcc gcgcgcgttg cttcgtgaca
    10201 tgagcggagc ggacatcccg gtgccttcgc cccaggatcc cggcgactgg agttcgcccg
    10261 ggcacatcca ttcgctggtg ctgatggcgg tgaccctggc cggcctgtac ctgtgctacc
    10321 ggatggcgtt gcccttgctg cctgcactgg cctgggcgct ggcgttcgcg ctgctgctgg
    10381 tgcccgtgca acgctggctc gagccgaggt tgcgctcccc cggcaccgcc gcgggactgc
    10441 tggtcctgct gggcggggtg ggcgtcctgt tcccggccat gctggtcgcc gaccggctgc
    10501 tggacgaggt ggcccgtggc ggcggcgcga tcaccgcgat gctcgagtcc gggcagtggc
    10561 gcagaagcat ctccgcctat ccgctgctgg tgcccgccgc cgactggatg gaagcccagt
    10621 tcgacctccc ggagaccgcg aacgcgatga ccgcatggct gaccacggcc gtggcgtcgc
    10681 tggcccgcga gtcgctgctg caggccatcg gcatgatcct gaccctgtac ctgctgttct
    10741 atttcctgcg tgatcgacgc gccatcctcg cgtccatcga ggccttgtcg ccgctggccc
    10801 gcgcggatac gcagcgcctg ttcggggtgg tcgacgacac cgtgcacgcc acggtgtacg
    10861 ggacgctggt gatcgcgatg gtgcagggca cgctgggcgg gctgatgttc tggtggctgg
    10921 gcctgtccgc accgttgttc tggggcgtgg tgatgggcct gctggcgatc gtgccggtgc
    10981 tgggggcgtt catcgtctgg atcccggccg cgctgttcct gctgctggac ggcagtggcg
    11041 gcaaggcgct ggtgctgacg ctttggggcg cgatcgtggt tggcggcatc gacaacctgc
    11101 tgtacccgat gctggtgggc aggcggatgc agatgcacac cgtgctggcc ttcgtctcga
    11161 tcgtcggcgg tttggcgctg ttcggatccg cgggcctgat cctgggcccg gtgatattcg
    11221 ccatcacccg cctgctgctg gaaatctgga gccgccgcgg cgagcgtgtc gccgcatccc
    11281 ggtacacggc cgagccgatg cctcccggag acacaccatg aacacactgg atcgcccgcg
    11341 ctacaccccc acgctccggc gcctgcactg gctgatggcc atcctggtgc tggcgggtta
    11401 cctgctgatc gagcaacgcg ggctgttccc gcgcggcagc gcgggacgca gcgcgatggt
    11461 gcaggggcat ttctgggtgg gcctgctggt gttcgtgctg gtttggtggc ggttgctgcg
    11521 ccgtcgggcc gatggcgcgc cggcgatcac gccgccgctg gggcgcgcca ctgcgctggc
    11581 ctcccgggcg ttccacgtcg cgctgtacgc gttcctggtg gtcatgccca tgctcgggct
    11641 ggccacggca tggagcgacg gcaaggccgt cctggtgcca ttcaccggga tcgcgctgcc
    11701 ggcgctgctg cccgagaacg aagcgctggc gcacaccctc gaagacctgc acggcagcat
    11761 cggcgaagcg ttctactggg tcatcggcgc ccatgtgctg gctgcgctgt ggcatcacct
    11821 ggtccgccgc gacgacaccc tgcgcaggat gctgtagccc gcgcgcctgc ggcagcgatc
    11881 ggttcgtcgg cgctgcgggt gccgccagtc ctgcgttagt ctggcgcgca caccagcagc
    11941 aggcggcgac atggacaggc gaggcttcat ccggactgcg gcaatgacgg cggtgctgcc
    12001 gtcgctcggg ggcatcggcg cggcgagcgc ggcagcaaga ccgcgcctgt tgccggtgcc
    12061 gctcgccaag ggcgacacca tcggcctggt cagcccgtcc tcggccaccg acgacagcct
    12121 gaacctgcaa ctcgcccgcg aggcgatgga ggcgctcggc ttcacggtga agaccggcgc
    12181 gcactacgac gcccgccgcg gccacctggc cggcaccgac gcgcagcgtg ccggcgacct
    12241 caacgcgatg ttcgccgaca aggcggtcaa ggcgatcgtc tgcacccgtg gtggctcggg
    12301 cgcggcacgg ttgttgccgc tgctggacta cgcggtcatc cgccgcaatc cgaaggtcct
    12361 gctcggctat tccgacatca ccgcgctgca ttgcgcgatc caggcgcagg ccggactggt
    12421 gaccttccac gggccgatcg gctcgggcag ctggaacccg ttcaacgtcg accagttcag
    12481 gcgcgtgctg ctggaccgcg agctggtcga ctaccgcaac aaggtcgagg ccggcgacga
    12541 gctggtgccg cgtcgcaacc gcacgctcac gatccggggc ggcaaggcgc gcggcgagtt
    12601 gatcggcggc aacctcacgg tgctgacggc gctggcaggt tcgccgtacc tgcccgactt
    12661 ccgcggcagg atcctgttcc tggaagacgt ctccgaagcg ccgtatcggg tcgaccgcat
    12721 gttcagtacg ctcaagctga tgggcgcgct ggaccaggtg gccggggtga tcttcggcga
    12781 gtgcagcgac tgcaatccgg gcgacggcta cggctcgctg acgctggagc agatcctcga
    12841 cgactacctg ctgccgctgg ggatcccggc ctaccgcggc gcgatgatcg gccacctccg
    12901 cgaacagttc atcgtgcccg tgggcggcaa ggtcgaactg gatgcggatg ccggtacctt
    12961 ccgcctgctg gagccggtgt tccaggcctg agtggccagt ctcgcgggca cctcgcgttg
    13021 gcagggagac cacccacgcg aaggagcctg cacatggcca ggaagaaagc cggcgacacc
    13081 acgccctcca ggaagccgga catcaccgtc gggaaaacgc cgcgcccgaa gctggcgacc
    13141 ggtccgctga cggtcaccgc gcagggcgcg atcctcaagg acgacgaccg cctgatcatc
    13201 gtggtgcggg tgctggacga cgagggcttg ccggtgaccg gcttgaagaa ggccaacttc
    13261 aaactgtggc agatggccca cctgttcgac cagtcgacca gcttcttcgt ggtcgagatc
    13321 gagaacatcc ccgggctgga aggcatgtac cacctggtgc agaagacctg gtcggtggtc
    13381 ggcaacggca ccatcccgtt cttcgtgcgg gtggagaagg gcaagttgcg cagcggcgcg
    13441 gcgctgacct tcatcgtgaa ggtgcgtgac ggcctcgacc tgtagccggc gggccggggc
    13501 ggctaccatc cgcccatgcc catccgcccc gtcgaccccg ccagcctgcc gccgtggaag
    13561 cgcaagtcgc cgcccggcaa atcgcgtccg ctgagtgcac tgcagatcga tgccgcgcgc
    13621 gagcgcgccg cgcaggccgg acggcgctac cccaacctgg tcgacaacat gtgggcggcg
    13681 cgccacgtgc cgcgtggcga cgccgaccca gcggcagggg gcgacgacga agcgtgagga
    13741 tgtccgcgtt cgcgtcgacc ttgcgtaact cccccggtcc gaatgccctg ggtgatccac
    13801 gatgctgagc ttgtccttct cgcgttccct gcgtgttttg gcctgttgcc tcctgctcgt
    13861 cctgggggcc tgcaagccgc agcaacccgc gcctgccgcc agtgccgacg ccgccacgcc
    13921 ggcagcgctg ccggctggcg caggaccggc gaaggacgag gcctggccgg caccggacaa
    13981 ggtcgaacgc gacggccccg atgcggaact ggtggtgcag gtgggcgacc tcgacaattt
    14041 cggcttcggc ttcccggacg ggttcgatcc gttcaccggc aagtccacgc cggtgcatcc
    14101 gttcccgttc cagcccggcg ccaaggacgc acgcggcacc gaccggatca tggtggtcag
    14161 tggcttcaag gccgccgacg gcgatggcta caccagcggc accgagcgcc ccggcaacct
    14221 gccgcaagcg ctgcgggtcg cgttcgacct gggtggcatc gaggtcaccg gcgcggccct
    14281 gcaattgttc gtcgacgatt tccaggcacc ggtctggggc acgcgctacc aggcgcggat
    14341 caacggcgtg gaggtgccgc tgctggcgac cacgctcaac gcgctcgacc agaccggtcc
    14401 gatcggcaag ctggtcaccg tgcaactgct gccggagcaa ttgagcctgc tgcgcgaagg
    14461 gaaactggaa gtcgccatcg acgatccgat caacgacgtg gccgacggct tcgccttcga
    14521 cttcgcccgc ctgctggtca acccgaagcc gtggcgctat gccggcacga tccacggcat
    14581 cgcggtggac aaggccacgg gcgaaccgct ggcgggcgtg ctggtctcgg ccagcaatac
    14641 ccgccaggcc acgacctcgg ccgatggcgc cttcgtgctg gaaggcgcgc cggccggcct
    14701 ggccgtggtc tccggcagcc atccggacta ccaggcggac accgagacct ccgatcttgt
    14761 cgccggtgag cgcatcgagg tccggctgga actggaaccg tcggcgaagt ccggcgagag
    14821 tctgggcgag cagctcgaca agcagggcaa ggtcgacctg tacgggatct atttcgacac
    14881 cgacaaggcc acgttgaagg acgaatccac ggcggtgctg gaacaggtgc tggcgcttct
    14941 ggaagcgcgt ccacagctgc cgttgatcgt gggggggcac accgatgccg atggcagcga
    15001 ggcgcacaac ctgaccttgt cgggccgtcg cgccgaggcg gtcgtggcct ggttggtcga
    15061 acatggcgtg gatgcctcgc gcctgcgtgc agagggccat ggtgaatcgc ggccggtcgc
    15121 caacaacgag accggcgaag gccgcgcgct caatcgccgc gtcgagctgc gcgatgcgag
    15181 cgaagcgggt ggtgccgctc gctgagtgtc gccagccatg cgtccgtcag cgcaccttgt
    15241 cgaaacgcat gccgtccagc acgagtccct ggaccttgcc ggcatcgacc acgaattcga
    15301 gcacctggcc tgagttcggc acgaactcga cgcgcacgtg gtcgggcttg tcgtagccgc
    15361 tggccacggc ctgcagctgg ccccagcgca gcgccaatgc gtcgtcgtcc tgtcgcgtca
    15421 ccgacatctc gccgaggtcg gcatgcacgt agcggcccgc ataggtctcg cgcggcagcg
    15481 agaggcgcca gggtcgcgcc cgcaaggcat cgcgctggcg gatcgcggcc tgctcaagcg
    15541 ttgccgtgtc ctccgcgagt tggacgaagc gttccttcgc ctttgtcgcg atctccggtt
    15601 cgtccagcgc gatgccatac gcgcagtcgg cgatcaggtt ggtcaggcgc gcgcccagca
    15661 cgtcctcgtt gttcaggacc accaggccga tgttggcgtc cggcatgaac gacaggtgcg
    15721 cgtggaagcc ggcgaagccg ccgaagtggt ggagcatgcg ccggcccttg tagtcgccgg
    15781 tgtaccagcc ccaggcatag ccgtcgcgcg ggaagtccag gtacttgctg tcggtggtcg
    15841 cctgtcgttg ctgcgacgca tggatgactg atgccggcag gcgctgcgtc ccatccagtt
    15901 tgccgttcgc gagttgcgcg atgaggaagt tcgccaggtc gggcgccgtg gacagcatgc
    15961 cgcccgcggc atgcatggtc gcgtcggtct tgcgcaggta gagcggcacg gtccggtagg
    16021 cggagacgaa ggcatagggc ttggcgacgg tccatccacg tgcgtctgcc tggctggcaa
    16081 gcgcggtggt gtggcgcatg cgcaacggcg cgaaaatgcg cgcatcgagt tgcgcctgcc
    16141 atggcgcggc gtcgatgcgg tccagccaca cgctggcgat gttgtagccg atgttggtgt
    16201 agtcaaaggt tccgagcggc gattccgcat tcggtcggga ctgcaggaca aggcgttgac
    16261 gcgaagtcgc atcgtgcacg ccgctgaacg cggtcgccca acccagcgcg acgttgtcga
    16321 tgccggaggt atgggtcagc aggtgccgca gggtgaccgc gctgggatcg acctcgctga
    16381 aatgcgcgtc gggaaacatc gcctgcagcg tggtgccggt atcgagccgg cccgcgtctt
    16441 gtgccagcaa tgcattcaag gcgaagaacg gcttggtcgc cgaggcgatg tagaacacgg
    16501 tctcggcatc caccggggtc ttcgccgcga tgtcggcgaa accgaagtaa ccctggtacg
    16561 caatcttgcc gtccttcacc acgatcaccg cagtgccggc cgggtaacgg gtgtcttgct
    16621 tgaactggtc gatggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgat
    16741 caccgcagtg ccggccgggt aacgggtgtc ttgcttgaac tggtcgatgg cttgcgcgag
    16801 tgcgttgaag ccagcgggat tcgcgggcgc ggcttgcgcc tgcggcagca ggatggccaa
    16861 ggcgagcggc aaccagcgag caaacacgga gatgcgaggg tgtgtcgggg aagcgggcgt
    16921 tgcgtgcatc ggggcgacct gcaatgagga tgcagcaggg atacgggtgc agggcgatgg
    16981 gttgcagcga ctgtgggacg acgcggcaca atcgcagggt ggagccgaac tcgcacctgc
    17041 caaggtcgga tgacgacttg cccgtggtcc ggcatggatt tccctggccg ctgcggctga
    17101 tcgtggcggg gttcggtgtg ttcgcgatgg tcgtgcctgc gtgggaactg gggcgcgggc
    17161 tatggccgtt gtcgatcgca acgccggtgt tcgggatcat cgtcatcggt gcggcgagcc
    17221 tgggcatcgc catgctgcgt gcggcgctgg cgggcgagtc gcaggtgtgg acgtttccac
    17281 cgaatgccgt ggtgatccat cacaaggcct ggcgcaacgc gtggatcgtc cgactgaccg
    17341 cgaacaacat cgccgcgatc gaggtccggc gcctcggcgc cagcgagggc gaagacatct
    17401 ggcgcgtcgt gatcgtgccc aaggccagcg attccgggtt gcgcatggcc gcccacgacg
    17461 gcgggttcga aaccggcgac tacaccagcg cggcgtatgc ggagaaggtc aggcgcgcct
    17521 tgctggcgca cctcgggatg cgctgaaagc cgatcgtcag gccaggtgcc ggcggaacca
    17581 cgacagggtg cgttcccatg cgagcttggc cgcggcttcg tcgtagcgcg gggtggaatc
    17641 gttgtggaag ccgtggccgg tgccgggata gacatgcgcc tcgtaggtgg tgccggcggc
    17701 cttcagtgcg gcctcgtagg caggccaggt cgcattgacg ttggggtcgg tctcggcgaa
    17761 gtgcagcagc agcggtgcct tgatccgcgg cacatcctcc ggcttggcct ggcggccata
    17821 gaacggcacc gccgcggcga gctccgggta agcgaccgcc gccgcattgg atacgccgcc
    17881 gccgtagcag aagccggtaa tcccgacctt gccggtggcg tcgggatcct tcgcaaggaa
    17941 ctcgatggcg gcgaagaaat cgttcatgag cttggcggga tcgacctgct tctgcagttc
    18001 gcggcccttg tcgtcgttgc cggggtagcc gccgagcgag ctcagtccat ccggtgccag
    18061 ggcgatgaag ccggccttgg ccacgcgccg cgccacgtcc tcgatgtagg gattcaggcc
    18121 gcggttctcg tggacgacca ccacgccggc cagcttgccg tcggcctttg cggggcgcac
    18181 gcggtaggcc cgcacctggc catggccatt cggcgaggga taggtgatgt attcggcgac
    18241 gatgtcggga tcggtgaatt tgacgtgctg ggcccacgca tagttgggcg tgagcgcggc
    18301 gagcgcaccc actgccgtca tgccgcccag ggccagcagg ctggcgcggt cgaggaactg
    18361 gcggcgactg atccggccat gcacgtagcc gtcgtacagc tcgagcaaag cgggcgggaa
    18421 atcgcttgcg gtcatgcggg tcatggcgtg gctcctgcgg cggcgcgata cggccaacat
    18481 acccgagctg cgtcgtcgcc gatgcacggg gcgggcgcga tcgcgcgcga tcggcgaaaa
    18541 tggcggctct cgtatcgact ggtcaccgca tgctgcccct gcgcctcgcc ctggtcttcc
    18601 tgatcatcgc cggcaacacc ctgttgcacg tgccgccact ggtggcgatg gcgttcatca
    18661 aggcgctgct gccgtccgag cgcctgcgtg tcgccagcaa tccgctgctc accggcctgg
    18721 cggaaagctg gatcggggtg aacaactgga tgtggcggac cttcacccac acgcgggtgg
    18781 acctgcgcga agacgccggc ctgcgcatcg atggccatta cctggtgctg gccaaccacc
    18841 agagctgggt ggacatcctg gtcctgcaga aggtgttcaa ccggcggatc ccgttgatgc
    18901 gcttcttcct caagcgccag ctgttctggg tgccgctgct ggggctcgcc tggtgggcgc
    18961 tggatttccc gttcatgggc cgctacacca agaagcagat cgcgaagaac ccggacctcg
    19021 cccgtcgcga catcgatgcc acgcgccgcg cctgcgagaa attccgcgcg atcccggtgg
    19081 cgatcatgaa tttcgtggaa ggcacgcgtt tcacccaggc caagcacgat gcgcaggggt
    19141 cgccgtttcg gcacctgctg aagccgaagt ccggtggcgt ggctttcgtg ctggacgcga
    19201 tgggcgaggg cctgcattcg atcgtcgacg tcaccatcgc gtatccggcg ggtacgccgt
    19261 cgctactcga cctgctcgcc gatcgcattc ccgaggtccg ggtgaaggtc cgcaagcgcg
    19321 acatcccggc cgacatcctg cgcggcgatt accagaacga tcgcgccttc cgcgtgcagt
    19381 tccagcagtg gatgaacggg ctgtggcagg acaaggacgc ggacatgacg cggatgctgg
    19441 gacaggccgc gagcggcgac accggacgcg attgagtcgc gttgcctgca ggccttcggc
    19501 gacaatgccg atcccatcca ggagttgccg atgcccgttc cgtccatgtc gccgagtggt
    19561 cgcctgttcg ccgccgccgc gttgctcgaa ggcctgacct gggccgggct gctcatcggg
    19621 atgtggctga aatacggcat gcaggtgacc gacaccggcg tgtggctgtt cggccgcctg
    19681 catggcggtg cgttcctgtt ctatgtcgtt gtcacggtga ttgccggcgt gcgcttgcgt
    19741 tggccgagct gggccttgct gctcgcgctg ctggcagcgg taccgccgtt ggtcacggtg
    19801 ccgctggaga tgtggttccg ccgcaaggga ttgttgtcgg atccggcgac gcgctgagcc
    19861 cgcgcctcaa cggcgcacgg ccagcacgcc atccagcgcg agctcggcgc ggaagccggc
    19921 cttttccagc cgcttcgcca cttcgcgcgg gacatcgcgc ccactggtca acttgttggg
    19981 cgaaccggtg taatggaaca gccggccgcc tcgcttcagc acgcgcgcga gctggtcgta
    20041 gaacacctgc gaatacagct caccggcgat gccgaagcgt ggcgggtcgt gcaacgcggc
    20101 atcgaacgcc gcatcgggca aggtcgtgat cgcctgcgag acatcggcat gggtcagttg
    20161 caaccggcca ccgtgttccg gtgcatcggg atcgggcgac cacgggttga gcgtgcgcag
    20221 ccacagcacg tcctcgttct tctcgaacga ctggatgcgt gccgcgccgg cagccaggca
    20281 gcaggcggcg aaatagccca ggccaccaca ggtgtccagc accgccttgc ccgccggttg
    20341 caccagcgcg accttctgcc tcgcgtcatc gagcggcgag gccttcgacg tcggcagcat
    20401 cttgatgccg tcgatctcga aggtcggcac gtcccattcg gtcggcacca gcttgatcag
    20461 cttgccggca tagcgcgaaa tgggcgcaaa ttcgtcgcca tcccaccagt acagggtgcg
    20521 gtccttgagc gtgtcggccc agggatagcg ctggccgcgc cactcccaat gcgtctcagc
    20581 cagcgtcgcg gtgtcgcggc tgcggccaag gtcgaacgag ccggtccagg cggtgtcgcc
    20641 ggcatcgcgc gcggcgcgca gggcatcggc aagcgggcgg gtgagcaggg ggccgtggta
    20701 acggggcaag cgcgatctcc ggggcagggg cggcatgcta acccggcccg catcgcgccc
    20761 tttggcccca cctttggcag gggaggcttt cagctggagc gggtaggctg gccctttcca
    20821 ctccaaccgg acgccgccat gcgccttgcg ccattgacct tgagcctggg catcgccctg
    20881 gcgttgacca ccgttgccgc gatcgctgcc cagccgggcg ccagcaacgc gcagatcggc
    20941 agtttcggcg tggacctgtc ggcgcgcgac accacggtga agccgggcga cgatttcaac
    21001 cgctacgcca gcggcaagtg gctggacagc taccagctga aggactacga gaccaacttc
    21061 gggtccttca acgcgctgcg cgaccgcgcc gaggaacagg cgcatgcgct gatcgaggaa
    21121 ctgggcaagc gcaacgacct gaaggccggc agcaacgagc gcaagctgcg cgatttctac
    21181 gccagctaca tggatcgcgc cggccgcgat gccgctggga tcaagccgct gcagccggtg
    21241 ctggaccgct acgccgcgat cgattcgaag gccaagctgg tcgccgcgtt cggcagcgac
    21301 gacatcgacg gcggcaacgc gccgatcggc atcggcctgg gcgcggaccg caaggacccg
    21361 gatcgctacc tggcgtcgct gggcgtcggc ggcctgggcc tgccggaccg cgactactac
    21421 ctgaatccgg acgcgcgctt cgtcaagatc cgcgaggcct acgtcgccca catccagcgg
    21481 atgctggcct tcggcggcat caacggtgcc gatgccaagg cgcgtgcgga agcgatcctg
    21541 gcgctggaaa ccgcgctggc caagccgatg tgggagcgcg ccaagctgcg cgagcgcgac
    21601 aagacctaca acgtcgtgac cttcgccgag ttgcagaagc agtacccggg ctatgactgg
    21661 gcggcgcacc tgcgcgcgca gggcatgcag gcgccggacc gcatcaacgt ggtcaccccg
    21721 gatgcggtgc agccgatcct ggacgtgatc gatgcgacgc cgctggcgac ctggcgtgat
    21781 tacctttcgt tccacgcgat cgacggcaat gccggcctgc tgagcaaggc gatcgatgac
    21841 gcctcgttcg agttcaacgg caaggtactg ggcgggcaga aagcccagcg cgacgactgg
    21901 aagcgcgcgg tggcgctggt cggtggccgc gccggcctgg gcgaagccct cggcgagctg
    21961 tacgtggcgc gctacttcaa gccggagtcg aaggcggcga tggacaagct ggtcgagaac
    22021 ctgcgggccg cgctgcgcca gaacatcgcc gggatcgact ggatgggtga ggccaccaag
    22081 gcccaggcct atcgcaagct ggagacgttc cgcccgaaga tcggctatcc gtcgaagtgg
    22141 ctggactatt ccagcgtgtc ggtggtgccg ggcaacgtgc tggcgaacgt gatggcgatg
    22201 cgccgctaca accgcgacga ccagaatcgc cgggtgggcc agaagaccga ccgcgaggaa
    22261 tggggcatga ccccgcagac ggtcaatgcg tactacaact cgagcttcaa cgagatcgtg
    22321 ttcccggccg gcatcctgca gccgccgttc ttcgacgtga acgcggaccc ggcggtgaat
    22381 tacggcgcga tcggcgcggt gatcggccac gaaatgggcc acggcttcga cgaccagggc
    22441 agcaagtccg acttcgccgg gatccagcgc aactggtgga ccgacgagga tcgcgcgcgc
    22501 ttcgagcaac gcaccaaggc gctgggcgcg caatacgacg cctattgccc gctgccgggg
    22561 caatgcgtca acggcgcgct gaccatgggc gagaacatcg gcgacctcgg cggcatttcg
    22621 atggcctata ccgcgtacaa gctgagcctt ggcggcaagc cggcgccggt gatcgacggc
    22681 ctgaccggcg accagcgttt cttcctgtcg tgggcgcagg tctggaaggg caagtaccgc
    22741 gacgaatcgc tgctgaacct gatcaagacg aacccgcatt cgccggtgat gtaccgcgcg
    22801 aacggcccgc tgcggaactt cgatccgtgg taccaagcgt tcgacgtcaa gccgggcgat
    22861 gccatgtacc tgccgccgga gcagcgcgtc cgcatctggt aagccgcgtt tggcgcggcc
    22921 gccgtcgcca tctccgcgaa ccggggatgg cgatggatgg atcgattact cgcctgcgtc
    22981 ggtcgccggt gcgtcggccg gggccgatcc tgccttggcg gcttcgcgct cggcgcgctt
    23041 cttggcgtct ttctcgtctt tcttcttctg cttggccagc tcgcgctggc gcttctcgaa
    23101 cgaataattg ggcttggcca tgggcactca cggggtgggg caggctgcca gtgtacggga
    23161 cgcccgcgcc gggtacccag cgggcggcgg cggttaccat gcggattccc tcactcccgc
    23221 gagttccctg cccatgatcc agcgattcga cgccggcaag cgcatgtccg aagccaccgt
    23281 ccacggcggc atcgtctacc tggcggggca ggtgccggag gacaccagcg ccgacgccga
    23341 aggccagacc cggcaggtgc tggcggagat cgatgccttg ctggagaaag ccggcagcga
    23401 caagcgcagg atcctgcgcg cgcagatcta cctggccgac atcgccgatt tcgacgccat
    23461 gaaccgggct tgggatgcgt gggtggtacc gggcgaagcg ccggcccgcg ccaccgtgca
    23521 ggcgccgttg gcgcgggccg gctggaaggt ggaaatcgtg gtcaccgccg ccctctgatc
    23581 gaaggaaacc gtcatgtccg gacaacccta tccgatcggt gtccagggcc agccctgggg
    23641 cgccgccgag aaagccgcgt ggcgtgcgcg ccagcgcaag ctgcgcagcc atgccgatga
    23701 ggtgatggcg aagatcgatg cgttgcgcga tcgcttcgac gtggaacagt acggcgaact
    23761 ggattacgcg ccggacggca cctatccgct gctggcgatc cgcagccgcg gctgggacga
    23821 cggcctgccg acgatgctgg tgaccggcgg tgtgcatggt tacgagacca gcggcgtgca
    23881 gggcgcgctg cagttcgtgg aacgcgatgc cgccgcctac gccggtcgcg ccaacctgct
    23941 ggtggtgccc tgcgtgagtc cgtgggccta tgagcggatc aatcgctgga acccggatgc
    24001 gctggatccg aaccggaact tcaagcccgg cagtccagcg cgcgaatcag cggcgttgct
    24061 ggccctggtc gagccgctgg cgggccgcat cgccatgcac atcgacctgc acgaaaccac
    24121 cgacagcgat gaatccgaat tccgcccggc gctggccgcg cgcgacggca aggcgtacga
    24181 gcccggcacc atccccgacg gcttctacct ctgcgccgac agtgcgcggc cggaacctgc
    24241 gttccagcag gcgatcaacg atgcggtcgc acaggtgacc cgtatcgcac ccgccgatcc
    24301 ggacggcacc atcatcggat ccccggtggt cgcgccgggg gtgatcgagt acgactgtcg
    24361 cgcacttggg ctgtgcgccg gcatgaccga tgcgcgctat gtgaccacca ccgaggtcta
    24421 tcccgacagc ccgcgcgcca ccccggcgca gtgcaatcag gcgcaggctg ccgcggtggt
    24481 cgcggcgatc gatttcgcgc tggcccacca ttagacgcgg cgcgaggaac cggccatgga
    24541 tcgacgcacc ttcctgcagg gctcgctggt ggcggcggtc gccgccgcga tgccgggcga
    24601 agccgaggcg cagtcggcgg cagcgccggc cttcccgcca atcgatgcgc gtagcgtgtg
    24661 gctggtcggc gacgacgcac cgcctgatcc ggtggcgatg accacgcgcc tggcggagct
    24721 ggcgcgcacg caccccgagg tgcgcgaccg ctaccagcag aaaggcgcgg tcgaggcgct
    24781 ggagcgcgcc ttcgcgacgc tactgggcaa ggaggactgc gcgttcttcg ccaccggcac
    24841 gctggccaac cacgtggcgg tgcgggtgct gtgcggcgag catcggcgcg cgctggtcca
    24901 gcaggagagt catctctatc gcgacgaaag cgatgcggcg cagcgccttt cggggatcaa
    24961 cctggtgccg ctcgccgccg gacgcgccag tccgtcgctc gatgaagtgc gcgccgcgat
    25021 cgacgcctcc gagaacggcc cgtatccgct gaagatcggc gcggtctcgc tggagagccc
    25081 cgtccgccgc gtggatggcg agatgctgcc gttggcgctg gtccgcgaca tcgccagcct
    25141 ggcgcgtgcg catggggcgc gcctgcacct cgacggcgcc cgcctgctgc tggcgcgacc
    25201 ggacctggac atcccggcat atgccgcgcc gttcgacacg gtctatgtct cgctctacaa
    25261 atacctgggc gcgccattcg gtgcggtgct ggccggatcg aaggcggaga tcgccgaagc
    25321 ccgtgacctg cggcatgtgt tcggcggtgg gctctaccag gcctggatgt cggccgtact
    25381 ggcgctggac gcgctgccgg cattccgcca gcagatcggc caggcgcacg cgcgggtgcg
    25441 caagctgatc gacgcgctgg tcgccagcgg cagggcgcgc gaacgcagcg ccgcggaggc
    25501 ctcgaacatc ttctggctgg agatgccgca ggcgttggcc gaagccgcgt tcgagcgcgg
    25561 acgccaggcc ggcatccgcg tcggccgctg gcaggacggg cgcatcccgt tgcatgccaa
    25621 caccaccatc ctgcgcaggc cactggaaga ctacctcggg ctgttcgggg gctgaacctc
    25681 cgggcgatca gcccagcact tcgcgctggc tcaagcgcca cagcatcgcg gcgaacaccg
    25741 cgcagccgcc gatgcagccc aatgccaggg tgaactggac gcgcagtgga tcggtggcga
    25801 gctggttgac cgggatctgc cacggcagca ccgcgccgac cttcgccgag gtggccacca
    25861 ccgcgaagaa ggtgccgccg atgcccaccg ccagcgccgg cacgaagctg gcatggcgca
    25921 gcgcgatcca cagctgcacc gccaccagca gccacgcggc caggaagata cggccaagca
    25981 acgccaggta gccggcaatg tcgggcgcgc cgattgcggc gaccgccggc ttgatgacgc
    26041 ccgcggtttc gaccgcaagc agggtcagca gggccagcca caggctcatc gccgcgacca
    26101 cgccgagcac gcaggcggcc ttgcctgcgt acaggtgcca gcgcggcacg ggcagggcgc
    26161 gcagatggtc ccaggcgcgc gggccatgtt cggtatgcgc gaccaatgcg gtgagcgcgg
    26221 tcacgctcat cggcagcatg aagaacgccc agatcgcagc ggaggtctgc aggctgatgg
    26281 cccatggcgc gcccttgtcc gcgcgcagca ggttgaagaa cacgaacacc gcgatcagca
    26341 gcggtgcgac cgcggccagc agcagggcca gcgatcggcg aagtttgtag gcctcgacgg
    26401 caagggcggt ggcgaagcgc ggagcgggga actgcgacat gtcgggtctc cttcgctcaa
    26461 gccgccgcag gcgcggcgga cagggcggca ttgcggtaga gggtctccag cgagcgctgg
    26521 cgcggggcaa gcgcatggat gcggacaccc ccggcgatgc acagcacccg gttgagcgcg
    26581 gaggtcgctt cgcgcacgtc ctcacccgcg gcgaagcgcg cgaccaccac gcccctgtcc
    26641 cgcatcgcct cgtcgcgcgc cacgtcgaag ccgtgctcgc gcgcgaccag cagcgcgcgt
    26701 tcgggggtgt cggtttcgac ggcgacctcc atggccaggc ccgccttgag gtccgccagc
    26761 gcgccttcca gcaccagccg gccatggctg aggatgccga tgtgggtcgc cgtctgttcg
    26821 atctcgccca gcaaatggct ggacaggagc acggtggcac cgctgcgcgc gggcagttcg
    26881 cgcaggaagc cgcgcatgtc ggcgatgccc tccgggtcca gtccgttggt cggttcgtcc
    26941 aggacgagca ccggtggcgc gcccagcatc gcccgcgcca ggccgagccg ctggcgcatg
    27001 cccagggaat agtcggcgac ccggcgacgg ccatgcgcgg tcatctcggc gacgtccagc
    27061 acgcggtcga tttccgatgc cggcaggccc agcaggcggc gggtgaggtc gaggttctcg
    27121 tggccgctga ggttggggta gaagccctgc gcctccagca gcgcaccgat cctgcgcgcg
    27181 gcgcgcaggc ggtcggtggc gatgtcgatg ccggctatcc gcgcggtacc ggcatccgcc
    27241 cggagcaggc cgagcagcag cttgatggtg gtggtcttgc ccgcgccgtt gcggcccagg
    27301 aagccgtaga tgctgcgctc gggcacggtc atcgtcacgt cgtcgaccgc cacgcggttg
    27361 ccgaagcggc gggtcagtcc ctcggtttcg atggcggatg gcatgcaggt gcttcccgga
    27421 aatttaagtc tgggataaag ttataggatg gcaaaaactt taagtcaaga ttaaatttcc
    27481 atgaccttcg acaggggtat cacatgccga ttaccgatcg tttccgtcgc cagtgccggc
    27541 tcctgcgcgc cgcgacgctg gtggtattcg tgggcctgtt cctgctgctg gccttgggca
    27601 tgatcgggct gccctggcgg atggcgaccg acatgcccgc aggctggagc acgcgcgcgt
    27661 tgttgtcggt ggtgcgcttc ctgccagggc tcggctacct gtgggcgttg tgggccgtgc
    27721 agcgggcgct gggcgacctg gccgcaggtc gcctgttcca tccgacggtg gcgcgcgcga
    27781 tgcggcacat cggcatcggc gtgctcgccg gggcgttgtt caacgtgttc gcggtgacca
    27841 acctgtcgcg ctggattgcg ggcgggcacg ggggatacct gtatttcgac ctgtccggga
    27901 tcgtgctggg cgtggtcggc gccgccctgg tgttgctggc gcggctggtc gaccaggcgc
    27961 gcgcgctgca ggccgagctg gacgagatcc tctgatgccg atccgggtga cgctcgacga
    28021 catgcttgcg cgccgcggca tgcgggcgcg cgaactggcg gccactgtcg gcatcagcga
    28081 aacgcagctg tcgctgttcc gcagcggcaa ggtcaagggc ctgcggttcc gtacgttggc
    28141 gaaattgtgc ttcgtgctgg agtgcacgcc aggcgaattg ctgggttacg accgcgctgt
    28201 cgccgacctg cagcaggtcg acgacgacgg gtgaggccat tgcaggcgac gcaaccgctc
    28261 agccgatgtc gatgtcgccg gtcttgtctt cgtaggcgcg cttcggatcc acgccgagca
    28321 tcagcttcac cccggcgagc aggctgttct cgttgagcca gacgtgggtt tcctccggat
    28381 ccatgcgcag caggcgcaag gtcgggtcgt ccttgccgcc ctcgaaccag gcggcgatga
    28441 agggattcca caggcggtcg atcaccgccg ggtcgttgtc gaccacgatc ttgccgctca
    28501 ccgtcgcgaa caggccgtgg tccttgctgg tcaccgtcgc ggtcgcctgc ttgcccatgc
    28561 tgccgcgcag gctctcggcc aggtctgtgc cggaggcggt gaagaaccac agcggtgcct
    28621 tgtcgtcctc cgccagtgcg gtcatcggcc gcggtgccac gccggtgatg cccagcatga
    28681 ccgtgcggtc gtggtcgatg gccttccaga acttgcgttc aaggtcgcgt gtgtcggtca
    28741 tggcgcattc ctttccttcc gtgggggagg tcaggaatac cagcccggcg gtgacggacg
    28801 cgccaacagt ccgtcgattc cgcgaaagca gatggaccgc ctcgtgccgg ccgtgcgggg
    28861 gatgccgatg gatgtcattc ccgcgtgcag gccatgtcgc caacacggca agaggatcaa
    28921 tgccgggtgg tggatgcgcg caatgcgccc gatggccggg cgcgtgtgcg ccggccgggc
    28981 acggggcgag tcctgccgat gccacgcgcg gcgccggcag cctagactgc gtccttccat
    29041 cccagcgaga ccggccatgc gcctgctgcc cttcgcgttg tcgctttgca tcggcacggc
    29101 cgttgccgcg gatcccgccg cgccggccac taccccgacg ttcgatgccg cgctcgccca
    29161 gcgcaccggc gcggacgaac ggggcatgcg ccgctacgtg ctggtgatcc tcaagaccgg
    29221 cccgacccgg gtaccggatg gcgaggcccg cgatgcgatg ttcgccgggc acatggccaa
    29281 catcgggcgc atggcaaagg acgggacact ggcgttggcg ggaccgttca tccaggaccc
    29341 ggccggctgg cgcggcctgt atgtgttcgc ggtggacgac atcgagcagg cgaaggcgct
    29401 ggtcgccacc gacccggtga tcgtgcaggg cgagatggtc gccgaatacc acccctggta
    29461 cggctcggcg gcgaacatgc tggtgccgga atggcatgac cgcctggtgc cgccggcaaa
    29521 acccgcgcag tagcgcgtcg cacgcctcag ccagcggaga tccgcacgca attgcgtccg
    29581 gcgtccttcg cctggtacag cgcgatgtcg gcgtcgcgca gcagccgttg ccggtcttcg
    29641 ggggtgtgca gcacgcggct ggccaccccg gcactgatcg agacccgggc gtcctgcatg
    29701 ccatcgggga tctgcacgcg catcgcctcg acggcggcac gcatgcgctc ggcgacatcg
    29761 cccgcccccg cagtcgtcat ccccggcaag aggcaggcga attcctcgcc accgtagcgt
    29821 gccagcagcg ccccggacgg cgtgcaggtg gacatcgcct gcacgatgcg ccgcagcact
    29881 tcatcgccgg ccggatggcc gtggcgatcg ttcagttcct tgaaatgatc gacatcgaag
    29941 aacaccagcg ccgcgatgct gccggcaggc gtgcgctcca gcgtctccag caggcgtcgc
    30001 cggttggcga ggccggtcag cgcatcgcgg taggagaggt cgagcaggcg ggcattggcg
    30061 gcgtcgagtt cggcggtgcg ctcggccacg cgttgttcga gcgcatcgtg ctgtgcgcgc
    30121 aggacacggg tacgcgccca cgcggcgaca tagcccagca gcaggagcgc cagcaccgct
    30181 gcggcatgcg cccaggcctg ctgccaccag tgcgcggcca cgtggatcgg gatatcgacc
    30241 ggcgtgctgg ggttgccggc atggtcgcgc gcctcgatcc gcagggtgta atcgccgggc
    30301 ggcaaggcgc tgaagctgcg cgagggttgc gcagtccatg gggtcggcgc attttccagc
    30361 cccacgagtt gggtgcggaa gcgcgactcg gcttcgtggt cccaggacaa cagggcgaat
    30421 tcgacgtcga tggtggtggc acccgccgga acccgtagcg ccgcgcccgg cttcggcacg
    30481 ccgtccacgt gcatgccgac gatgcgcagg ggcttgggtt gggtatcggc ggtcgcgcgc
    30541 gcggggtcgt acaccgccag gccaccgagg gtgccagtcc agaagcggcc gtgcgcgtcg
    30601 atgaactgcg cgctgccgtt gcattcctcg tggaccatgc cgtcgcggcg attgaaggcg
    30661 cgcgaggtgt agccgccctt gccgtccgcc gtgagttgct gcacgccggt attgctgcac
    30721 acgtacacgc gtccatccgg cgcacgcagc gcgccgtagg tgtagggatc gggcgggggc
    30781 ggcaggttcg ctggcaacac gcgcggccgg gtggcatcgc cgatgtcgac gcggatgatc
    30841 ccgacgctgg cgctgcccat ccacaggatc ggccggccac gcgcatccgg gtacaggcgc
    30901 aggccggaca actggtcgtc cgggaaaccc gtggaccggt cgaacagggt ccaggccctg
    30961 ccatcgaagc gtgccagccc gcggtcggtc gcggcccaca gccaggcgtg gccggcgcgg
    31021 tcgcgttgtt cgaccatctg gctgacccgc cactggccgt cgatacccgg tgcgcgcatc
    31081 tccatccagc ggccatcgcg caggcgccac gcaccggaca ggcgcgtgcc cgcccacagc
    31141 tcgtcgtggc catcgaccgt gcgcgacagc acgtcctgca ccgattgccc gttcaatttc
    31201 ggccatggcg tggccatccg gtcaaagcgc ggaccctcgt cgatgcgcag caggtccccg
    31261 ccccaggtgc ccagccacaa cgtgcggccc gcgcccgtgg cgtccggcag cgaacggatc
    31321 atgctgatgc cattgcttgg caggtgtccg ctgtcggcgc tgaagcgctg ccagcggccg
    31381 gcctggtaca ggcccagccc ttcgtcgctg gagccgaccc agagccgctc gccgccatgc
    31441 ccgtcgggct cgaccagcac gccgaacacg ccgaccgagc gcgcgcccat cagcgacgcg
    31501 gtggaccacg cgctgccatc cagcacggtg cgcgagatcc cggcttcggt cgccagccac
    31561 agcacttcgc ttccgctcgg actgcgccag gcattgagcc cgcggatcac gtccgaggcc
    31621 aggccgtggc ggcggtcgaa cacctgcacg cggccgtcgt gcacgcgcaa caggccggag
    31681 cgcgaggcga cccagatcgc ccgcttgccg ttggccagcg gagtagtcgc gatgtcgtag
    31741 agctggttgg tcggcaggtc gcccttgccc tgggtccagc gctgcatgcc acgcgcgtcc
    31801 agccgcatca ggcccacccc gaacaccgac atccacaggg cttcgccgtc ggcgtcggtg
    31861 gtggtcacca gcgattccac ctgcgccacg ttcacgtcgt cggcacgcca ttgcgtccag
    31921 tcgctgtggc cttcttcgcg gtaccacagg ccgtggctgc cgctgcccac ccattgccgg
    31981 cgtgcaccgc cgatcgtggt ggtgttcgcc agcgacaggg tcatgctgcg cggcagggtg
    32041 gcgttgccgg gatcggcgac ccagcggccc tgccggcgca gcatcaggcc accggtccag
    32101 ctggccgccc acagcgtggc ctggccacgc gcgtccaccg tttccgcgaa ccgtcgcacc
    32161 tggtcgaaag gcaagccggt ggtcgcgttc tccacgcgcc aacgctggcc atcatgatgg
    32221 gccagtccat ggtggcggaa cgcggcccac aggatgccgc tgcggtcgac gaacaggctg
    32281 ctgaccggat gcgccatcgc gggatcggcc tccgtgatcc agcggcggcc gtcgtagcgc
    32341 gagacgccct gcggcgaagc cgcccagacg aacccttgcc gatcggtgcg cacatcgacc
    32401 agcacctgtt cgggcaggcc gtcgcgcgcg gagaagttgg cgaataccgg cgcgccgagg
    32461 tccgacaggt ccagtggccg caccgccgtg gaggcggtcg cgggtggtgg tgcggtggtg
    32521 gccggcgcgg cccatgcggg caccgcaagg cacagcagca agcagactgc ccggatcaag
    32581 tgcatgcgtg gatgattccc ctgtgcgggc gccacgcaac atgcgcaccg tgagcggggc
    32641 gccgtggatc aaccgccgct ggctttcgcc ttcgccttcg ccggggtggc gcgcgcgagg
    32701 gcggtcaccg cgtccagcgt ctccttgacg tgcaggttcg gattcatcgc gtcatgcaca
    32761 tggacgatcc tgccgtcgcg agcaatcaca taggaggtcc ggctggacca gtccggcttc
    32821 atcgccagtg tcgcgtcgta ggacttggcg atcttcgcgc cggtatcggc cgcgaccgcg
    32881 aatttgcccg cgcatttctc cgtgtcgctg gagaattccg cgaggcgctc ggtattgccc
    32941 gacgtcacgc cgatgaccgt ggccccctgc gccttgaact tgtcgatcgc ctgcgagaac
    33001 gccgcggctt cgacattgca gccgctggtg aacgccgcgg ggaagaagta cacgaccacg
    33061 ggccccttct tcaatgcggc ggccaggtcg aaggtgaacg gcttgccggc caggtaggcc
    33121 ggcgccttga acagtggggc ttgggtgcct ggcttgagcg cggcgaagca gggcgcggca
    33181 agcaggcagg cgagggcgaa cgcggcgtag cgacgggtca tgcgggcggc tccggtgcgg
    33241 agccgccacg atacgccatg cgcgtgatgg cgctggtcag cgacgcttga ccggctgcgc
    33301 caggccgctg gcgatggcct cgtcgatcag gctgcggatg cggatgtcgg cgacgccggc
    33361 ctcgcgttcg atctggttgc ccaaggcttc catgtcgcgg ccgatcgcct ccatcggctt
    33421 cgcagcggcc tccatctgcc gtgccaccgc gtccatcttc cccgactgct gctgcatggc
    33481 ccgcgattgc tggtccatca cgttcgcgtg gcggtccatc tgccgtgaca gcacgtccat
    33541 ctgttccgac agcaggtcca tgtcgcgggc aagggcattc cggccggcgt catcggcctt
    33601 cgccatcttc ccggccaggc gggcctgctt ttcggccaac gcctgctggc gcgaggcgag
    33661 gtcttccagt tgccgccctg cggcgcgcat ctccggcgtc tccacaggcg ccatgcggtc
    33721 catctggctg cccagtgcgt ccagcttctg ttggtgcggc tgcatgcgcg cctgcagggc
    33781 gtccatgcgc acttccagcg catcgaccgg cccccaggcc ttgtccacgc tggccagcac
    33841 gccggcatcg cgcaccacgt aggcctcgcc gccgcggcgc acccacagga agtcgccatc
    33901 gatgccttgg cgcagtcggc gtatgtcggc cacgtcgtcg gtactcccgc tcatgcggaa
    33961 gccggtatcg cccttgcgca ccatggcgta gccgttcgcg gcgtccacgc ccgcatgcag
    34021 tttcttgacc cgatcggcgc gcggcggcgg cgggggcggc ggtgcgggca tcgcaggcgg
    34081 cgcgggcagt gcaggcgggg caggtggtgc cggcggggcc gggggcgggg gtggatgccg
    34141 ggtgtcggtg tgatcgacgg tcggagccgt ggcgggtgcg gtggattgcg catgcgcgta
    34201 catcgccgcg ccggccaggg ccaggcccag caggggcagg gacaggccaa tggcgagtgg
    34261 atgctggcgc gggcggagca ggtgctggat gcgggacatg agttggcctc catgggcggc
    34321 gtgcgcgaag cggggaatgg gcccggcggc ggtcgcgccg agcatgcggt cgagttcgga
    34381 cagcgcgagc gcaaggctgc gacgatcgcc caaggcgtcg gcggccaggt cgtcggcgat
    34441 cagttcgcgt tcgtggcgga tgcggcgcga cagccaccac accaccgggt ggtagaacag
    34501 cagggcctcg accacggcct gcaacaggtt gaccaggtaa tcgtggcgac ggatatgcgc
    34561 cagttcatgg gccagcagcg cctccagcaa accggccggc atgcgtgcga ccagcgcggc
    34621 ggggagcaac accatcggcc gccaccagcc gatcgccagc ggggcatcac cgtcgaacag
    34681 gcgcaggcgc acgttgtgcg gcaggccgag cttgcccgcc agcacgtcgg cacgagcttg
    34741 ccaatgcccg ggatcttcgg gccacgcccg tcgttgcagg cgacgcaccc accacagacc
    34801 gccggccatg cgcaggagca gggtcgcgac accggaaccc cagagcatga caacccacgg
    34861 catcgaggtc gtggcctgca tcgccaacac gtgctgcagg ccattggggt ttgcagtccc
    34921 cgcattcgcg cccaccaaca agggaagcga ttcggccgca gtgagtctcg gcgcagccag
    34981 cagccatgcc aacgtgagtg caggcagcaa cagcgaggca gacagcgcag cgcaggccag
    35041 ggcatagcgt gcctgcggac gtgcgttgcg cagcagggtc aagcccagcc cggcgagcag
    35101 gccgagcaac gcgccctgcc agaggaaatg cagcagcgcg ctgccccatg cgggaaccac
    35161 gctcgtcgcc atgtcattca tcgcgggtgt cctccaggaa ctgctggatc tcggcgcgct
    35221 cggcgtcgct gaccttgtcg ctgcgcagcg cggccatgac cagcgccttg ccggaaccgg
    35281 agaacgcctt gtggatcagg tcgtccagca ggcgcgactg cagcgagcgc tgcgcctgcg
    35341 ccaccgcata tacatgcgag cgctggcgct cgtcgcgctt gagcagcccc ttgccatgca
    35401 tgatctgcat cagccgcaac acgttggcgt aggccagctc ggggcgctca cgctgctgcg
    35461 cctcgtggac ctgcttgacc gtgctcggtc cgagttgcca cagggtgcgc aacagttcga
    35521 gctcggccgg cgtgggtttc ggctggcggg gcatgggcat tgcggtcacc gtcgtttcga
    35581 tgccgggacg atgccttttc tagaaagttt tgtctagaaa taattgtcta gatcgggaag
    35641 cgtccgacga acggtaggct gcgccgttta ctgcagcgga tcgcgttacc gacgacaccc
    35701 cgactttcga cataagcagc ctgcacccgc cgatgcgaga ctcgctgcac acaccagccg
    35761 ggggaaggca catgcgcagc acgcgtcggg atctgctcaa gttcgggaca ctcgccgccg
    35821 ctgcggcagc gctgccttcc ttcgcaacgc cgggcacggc cacggcaccg gtggccaggg
    35881 cggcgaagcc attggacatc ctgatcctgg gtggcaccgg cttcaccggt ccgttccagg
    35941 tcaactacgc gctgtcgcgt gggcacaagg tcaccctgtt caaccgcggc aagcggccgt
    36001 cgccggagtg gcctggcgag gtcgaacaac tgcatggcga ccgcaacacc ggcgacctca
    36061 agtcgctcgc cggccgcaag tgggacgtgt gcatcgacaa ccccaccagc ctgccgttct
    36121 gggtgcgcga tgccgccagg gtgctgaagg gcaacgtcgg ccactacatg ttcatctcga
    36181 ccatctcggt ctacgccgat ggcagcaggc acggcatcga cgaacactcg ccgctcgccg
    36241 tgtacaaggg cagggatgcg atggcagaga cgcaggaaag cctgcgtgcc gacatggaga
    36301 atctctacgg ttcgctgaag gcgctgagcg aggccgaagt gcgcaagcat ttcggagagc
    36361 gcaccacgat cgtccggccc ggctacatcg tcggtccgcg cgacgagacc gaccgcttca
    36421 cctactggcc gctgcgggtg gcgcagggcg gcgaaatgct ggtgcccggc gatggccagg
    36481 atccgatcca gatcatcgat ggccgtgacc tcggcgaatg gatgatccgc ctggccgaag
    36541 ccagggcgta cggggtcttc aacgcggtgg gcccggatta cacgttgacg acggatgcga
    36601 tgctctacgg ctgccaggcc gtcaccgcga atggcgtgca actcacccat gtcaccccga
    36661 agttcatcga agaccagaag gtcgaactgc cgatctgggt cccacgcgcg gataacccct
    36721 atgccggcta cggcacggtc agcaatgcgc gcgcattggc cgcgggaatg acattgcgac
    36781 cgctggcgac caccgtggcc gacctgctgg cgtggttcca ctcgctgccg gcagaccgcc
    36841 aggccaagcc gcgcgccggc atcacccgcg agaaggaagc cgaactgctg gcggcctgga
    36901 aagcgggcaa gtccggttag cggacgcgct gggccttgcg cagccgcttg atcgcgtaca
    36961 tcgccgtgtc cgcgagcgcc aggacgctgt ccacgtcgtg ctggtgctcc ggcaccagga
    37021 tggtgccgat actcgggccg tcatagtcga tggtgatccc gccgcccagg tcgaactgtc
    37081 cgcgcgtcgc ggtctgcaag cggtcgtgca gcgcgcccag ggccgcatcg gcttcggccg
    37141 ccggaacgct ggccagcacc acgaattcat cgccgcccag ccgcgcgcag tagtcgccgg
    37201 cgcgcgcggt atgcgtcaag gcttcgccga tgagcgcgag catgttgtcg ccggcctcat
    37261 gcccgtgcac gtcgttgatc gccttgaacc cgtccaggtc gatgaatgcg accagcagcg
    37321 cgccgtgctc acgatcgcgc cgggcgatgc ggcggggaag ttcctccatc accgcgcgtc
    37381 ggttcggcaa cagggtcagc gggtcggtca gcgcgctcat cgcaagggca tggttcgcgg
    37441 cccgcagttc ctgcatgagc cattcacgct cgacctgctg gccgatcagt tgcgagcaga
    37501 tgcgcagcac ccgcgccgca tcgtccgtcg ccggcctgga ggtatcgctg gccgcgcaca
    37561 aggttccata caggcggcca ccgggtccat ggatcggcgt gctcgcatag gtcgcgatgc
    37621 caagctcgcg cgcggcggcc gaatcgcccc agcgctcggc cacgtcatcc gaatacgcgc
    37681 gatcctcttc cagcgcgcgc ttgcacaagg tgtcgcccca gggcacggcc agcccttccg
    37741 ggatggtcat gcggctactg ttgcgcgcat acaggacatg ctgcaatccc gcggtctcgt
    37801 cgatcgcggt catgtaggtc gactcgaggc cggtggcact ttccaacaag gcaagcaggg
    37861 ggcgaacgag ttgttcgagc gaatgcgtgt cggtgatgga atccgcaagc tggacgatga
    37921 gccgccggtc gagttcgctg ccttccttca tggcgtggtc atgccgggta tccgcctcag
    37981 tgcgtggccg ccagtgtgcc atcggtgctg tgagtgctgg agacgcggtc gcgacccgcg
    38041 gccttcgccc ggtacagcgc gtgatccgcg gcctccatcc aggtgcgcag gtccgcatcg
    38101 gccgtcggtg gttctgcgct gccgatgctc accgagctcc gcagcatcgg caggtgcggc
    38161 aagtcgagcg tggccacgcc cgcccggatg cactcggcga tgctgcgcgc cttcccggcg
    38221 tccaccggca tcacgatcgc gaattcatcg ccaccgagcc gcccggcgtg gctgccttcc
    38281 ggcaacgacg cgcggatcac cccggcgata ccgcgcagga cgtcgtcgcc gatggcatgg
    38341 ccatggcgat cgttgatggc cttgaaccag tccacgtcga ccaggagcat gctcgaaggc
    38401 tggccggtgg cggcgtgcca ggccagcatg gctcccgcct gctgttgcca gtgatcccgg
    38461 ctctgcaatc cggtcaacga atcggaacgc gtgagttcat ccagccgcag gttctgcttc
    38521 tgcaccttgc ggaccaggcg atagctgctg gcgcnnnnnn nnnnnnnnnn nnnnnnnnnn
    38581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38641 nnnnnnnnnn nnnntccagc cgcaggttct gcttctgcac cttgcggacc aggcgatagc
    38701 tgctggcgct caccgcgagc gtatgaatga ccatcaacgg caggcaggcc agcaccaccg
    38761 gagggctggt ggcgatctcg acctgccatc cggcgagcag cgtcgacagc agcatcgcgg
    38821 ccagcatccc cagcagcgaa cgcgaccaca gcccgcgcac gccggcgttg atcttgtccg
    38881 cggtggcgac ggtgagcagc atcgcggatg gcagcaggtt gaaatgcatg atcggcgcca
    38941 ggctcccggc catcgcggaa tcgatcatga aattggtcag ttcggcacgg tacgggaccc
    39001 gactgtagtg ggcgcgcagc cgcgccacgt gcggccaggc caagccgcag aacagcgtcc
    39061 acgcccatgc aggccagccg gcatccagct cgtgcaggac caccaggacc gggaggaccg
    39121 ccaggcccat gcccagggtg cgcatcgggt atgtgcgtcg atgcaggttg cgcaggtgcg
    39181 gcggcacgcc gttaccgtcg gcgtcgtcgc ggtggttggt gcgtgtcatg ccggctcccc
    39241 ccagagcagc cgacagtgtg cgggaggagc cgcctcgacg ccaacgccac ccgctggttg
    39301 ctagtctccg tcgctcaaga catgggcggg aggacgccat ggcaaccgga tgggccggcg
    39361 acggcgcggt gcaggaccag atcgacgcga cggtgcagga cgccatccag cgtgcgcgca
    39421 ggcggctgcc cagcgggcct ggccgcatgc actgcgagga ttgcgaagcc ttgatccccg
    39481 cggcgcgtcg caaggcgatg ccgggcgtgc gcctttgcct ggcgtgccag gaactgcatg
    39541 atcgcgagga agcgggcgtc gccggctaca accgccgtgg cagcaaggac agccagttgc
    39601 gttgagcaac catcgattcg cagctcctga aagcaagacg cccggccgga gccgggcgtc
    39661 ttgtcgcatc gcgagggtcg atcagtagcg gctgatgttg aggatcgcgc ccaggatcgc
    39721 cggcaggatc accgcgaccc cgttgtcgta gcgcccgtac tggtagcggc ggtaatagcc
    39781 gtcgcggtac ccgcgttcga aaccctgctg gaaatagtat cggtattcgt tccggcccac
    39841 gtaatagccg ctgtagccgt acgtaccgtc catgtaaccg tagttgccct ggtagtcgaa
    39901 gcgccagcgg tcgttgcggt cggcctggcc agcgtaccaa ccctcgcggt aaccgtcgcg
    39961 gatcgcctgc tgcaacaggt cgcggccata actgttggtc tggtaccagc gaccgccgta
    40021 gctgtagcgg tagttgtacg cggtggagta gtacgggtcg ttgtaatagt cgtaacgcga
    40081 cgcgttccag cgtgactgct gcgccagcca gcggcggtag tagtcctgct ggtagcggta
    40141 ctggtggctg cggcgctggc gttcgagttc ccggttgcgc tgttccagct gcgaacggcg
    40201 atcggattcc cggcgttgcc actccatcgc ctggcggcgc tggtcgtcga tccgccgctg
    40261 ctgttgttcg cgggagatgc ggtcgttgcg gtcgtcacgg cgatcgtccc gccagtcgcg
    40321 gcggtcgtca cgacgatcgt cgcgtgcctc gttgcggtcg tcccggcgat cgtcgcgctg
    40381 gtcggcggtg cggcgatcct gttgggcgcg ctcgcgggcc atgcgttcgt cgttgtcgcg
    40441 gcgttgcccg tcggcacggc gatcagcctg ctgctggcgc tcccgctgct ggtcggccag
    40501 tcggcgttcg tcggcttcgc gctgcccggc ctgctcgcga cgctgctccg cgatgcgccg
    40561 ctgcatgtct tcgcgttgct cggcctggcg ctgcatgcct gcttcgcgct ggcgggcctg
    40621 cacgtccagg ttctcgcggc gctgctgttc gcgctcgcgg ctgccctggt cccagttctc
    40681 gcggcgctcg ggttgccagc ggccattgcc ttgctgcaag gccgggcgtg gctggtcggc
    40741 ctggcgctcc tgcacctgcg ggggttgccg ctcctggcgc tgcggttcct gccgctgtgg
    40801 ttgttcgcga ctcgactcct cgcggtcgac ttgctgcttg cgctgcttgc ggccgtcgcg
    40861 atcctcgtgc ttgtcctgct caagcgcgaa cgcgggggca gtagcgccaa tggcgagcaa
    40921 tgcggccatt agcgaaacgg ccagtcggcg cgcggtgaaa tggcgggtca tgatcttctc
    40981 cacctgatgc ttctccatcg gccgcgaacc attcgcgccg tgcgagcagt tagcggtgtg
    41041 aaggatgaat caatcctgag attttcgtta tgggaggtgc gcgaacgtgg acttgcaagc
    41101 tcgtattcag cttcggcagg cgtgtggctc gatggaagcg tttacggcgt tgccggcttt
    41161 tcccgccggg tccagacctt gttggcgatc ttccattcgc cgtcgacctt gagcagcacg
    41221 aagtaatcgg tgaaggtgcc ggagggatag tccagtgtga ccctggccat cgccgcgttg
    41281 ccgctgacat cgacacgatc gatccagcgt ttgcgttgcg cctcgtcggc gggcggtgcg
    41341 cccttgaacc cggcgatgta ttcgtcggcc gtccacgaca ggaacgcgcc atcgcggacg
    41401 ccctcgatgt gcgcggtcgg caggaacgcc tcgcgcatgt aggcggggtc gccggtggca
    41461 tggccctgca ggtaggcctc caatggcttg cgagcggcga catcctcgct cgcctgttgg
    41521 gcaaaggcgc tgcccggcat cgaaagcgag gcggcgatcg acagcagtgg aatcgcaagc
    41581 aggcgcatgg cggcatcccg gtggtcgctg tcgcgagcct acccgccggc gagccggcgc
    41641 agatgtgacg aaagacaggg tcgataccgg gccccacatg cgacagtgga agtccattcg
    41701 tccggaccct cgccatgccg cttgctcgtt gcctgcttgc cgtcgcactc gcctccgcca
    41761 gcgtggtagt gcaggccggg caacccgcgt tgacccgcac caccttggcg agtggcctgc
    41821 cgctcgcgcc ggagcagaaa gccgtggcct tcgagcgtgc ggagctctcg ttcaaggtgg
    41881 atcccgcccg ccgatggctg gagggcgacg ccacgctggc cttccgcgct gtgcggccga
    41941 tcgacaagct cgtcgtcgac ctggatcgca attacgcggt ggatggcgtc gaagtggatg
    42001 gcaaggccat tgatgccgcg cattggcgca atcccgaagg tcgaatgacc gtagatttgc
    42061 cgaagcccct ggcggtcaat gccgacgtgt tgttgcgcat ccgctattcg ggccatccgc
    42121 acgtggccga gaaagccccg tgggatggag gcttcgtctg ggccacggcg ccgaccggcg
    42181 agccctgggt cgccactgcc gtgcagggcg aaggttgcga cctgctgtgg ccttgcatcg
    42241 accatcccat gggcgagccc ggggaagtcg tgcagcacgt caccgtgccg agtccgctgg
    42301 tcgcggctgg caacgggatc gccaccggca tggaggagca ggagggttgg cgtacgtacc
    42361 actggcgcgc gaagcatccg aacacctacg cgatcgcgct caacatcggc ccgtacgaat
    42421 tgctgcaggg cgagtaccgg tcgcgctacg gcaacacgat cccgttgcgg ttctggcacc
    42481 tgaagggcaa taccaccaag gccgagggcc tgttcgccga attcgcgccg atgctggact
    42541 tcttcgagga gatgatcggg ccgtacccgt tcgccgacga gaagatgggc gtggtcgaga
    42601 ccccgcacct gggcatggag caccagacca tcaatgccta tggcaatggc tacaagaagg
    42661 acgagtacgg ctacgactgg ctgctgcagc acgagttcgc ccacgagtgg ttcggaaacc
    42721 agctgaccca tgtcgactgg gaggatttct ggttgcacga gggcttcggc agctacatgc
    42781 agccgctcta cgcgcaatgg ctgcgtggcg acatggcgta catggcggcg ctgatgaagc
    42841 aacgtgcggc gctggccaac aagtatccgg tgatctccgg gcgcggcctc ccggaaaacg
    42901 aggtgtacgc ggccgggcag ggcccgggca acgacaccta ctacaagggc tcgctgatgc
    42961 tgcacacgct gcgcggacag atcggcgacg ccgcgttctt ccgcgcgata cgcgagctgg
    43021 tctatggcac cgccacgccg cagccgggca acttcaagcc gcgccacgca tcgaccagcg
    43081 acttcatcgc catcgtcagc cgcatcaccg gcaaggacta ccgctggttc ttcgacggtt
    43141 acctccgtca tgcagccttg ccggacctgc aggccacgcg cgatgccgat ggtctgtcgc
    43201 tgcggtggaa gaccggtggt gaagcgccat tcccgttgcc ggtgcaggtg cgcgtcggcg
    43261 acagggtggt cgacgtggcg atgaccgatg gtcgcggcca tgtcgacctg cctgcgggcg
    43321 cgacctacac gctggatccg cattcgcgcg tgttgcgcga acttccctac atcgcggagt
    43381 tccagaagga cgccgcggaa cgcgcgaagg cggcggcgga gaaagccagg tccgaggcgg
    43441 cgaagaagcc ctgagtcgca ggcgaaatgg ggtcagagtg aagtttcgcg gccaagcgat
    43501 tgccataaca caagcatgat cggcgcgaaa ttcaccctgc ccccatttcg cgcagccaac
    43561 gtgtcgcgcc acgcccggcg cgcaggccgc tggcgaaaca cgcggtgagc aggtagccgc
    43621 cggttggcgc ttcccagtcg agcatctcgc ctgcgcagaa gacgcccggc gcatcgcgca
    43681 gcatcaatgc atcgtccatt gcctcgagcc gcacgccgcc cgcgctggag atggcttccg
    43741 ccattggtcg cgggcgcagc agccgcagcg ggaagcgctt cagcgtggcc acgatgcgtg
    43801 ccatgtcctg caatgccggc ttgtccagcg cttcgtgcag caacgcaagc ttcgcgccgt
    43861 cgatgccggc ctgtcggcgc aggtgttcgc cgaggctgcg cccatgtcgt ggcttcgcga
    43921 ggtccgcctg caagcgttcc ggcgtgcggc cgggaaccag gtcgaggtgc aggagcgcgt
    43981 ggccatcgcg tgcgatcgca tcgcgcaggt cggcggcgat ggcatagatg agactgccct
    44041 cgatgccggt ttcggtcagc acgcattcgc cctgcaacgc atgttcgata cccgcggcgt
    44101 cgcgccagtg cgcgaccacc ggcttcagtg gcgcccccgc atgtttgctg gacaggaacg
    44161 cgctccagcc gatatcgaaa ccgcaattgg cgggctgcag cgatgcgatg tcgatgccgc
    44221 gcccagtcag tatcctgacc catgcgccat ctgaaccaag ctgtggccag ctgccgccgc
    44281 ccatcgccag gaccaccgca tccgccacga acatcgtttc gcccccggcc gtggcgaagc
    44341 gcaatgcgcc ggcatcgtcc cagcccagcc agcgatgctg gacgtggaag tgcacgccgg
    44401 catcgcgcaa tcggcgcacc caaccgcgga gcaacggcgc ggccttgcga tccatcggga
    44461 acacccgccc ggaactgccc acgtaggtat cgaccccgaa gccgcgtgcc caatcgcgca
    44521 gggcatcgcc atcgaagtca tcgagccacg tgccgatctg cgcagtccgt tcgcgatagc
    44581 gcgcatcgaa cgtggggcgc ggctcggaat gggtgaggtt gagcccgccc ttgccggcga
    44641 tcaggaactt gcgccccacc gagcccttgg cctcgaacac gtggacgtcg atgccagccg
    44701 cgtgcgcgac ctcggcggcc atcaggccgg cggggccgcc gccgatgacg gcgaggcggg
    44761 cagggtaggt tgacggcggg gacatccgca catcatgccg cgactgcaac cgcccgcgca
    44821 cccgccgtat cccttgcgca acccgaagtg gatgccgtcg atgtcgcgct tgctgcctgc
    44881 cttgctgctg ttcctgttgc tgccgctcac cggcgtcgcc gcggattggg atggcaagcc
    44941 caaccgctac cgtgtgcggg tcgatgcgca gtccactcgc gcggtggtcg aggccgatct
    45001 ctggcagcag agcgacacgc tgtcgatgta caacgtgatg gaggtgccgg ggttgccgaa
    45061 cgggcaggcc agcctggtcg atggcatgcg cgtgaccgac gcgaaaggcg cggcgctcgc
    45121 gctggaggat ctaggtgcgg gcgactacgc catcgcgggg gggcgacgga tacggctgtc
    45181 atattcggtg cggctcgaac acgacaggta tccgtggcct gccgggatgg aagaggtgtc
    45241 gtatcgcacc gatgagggcg tgatggtgac cggtgcggcg ttgttcttcg ccgacggcga
    45301 tgcgccggtc aaggcgccga tcgaggtcga tttcgagctt ccccagggct ggcacgcacg
    45361 cacgccatgg acgcggattg gcggcggcga tcgcttccgg gtggattcgc gtcgcgaact
    45421 ggtcagcaat gctttgttcc tcggcaccgc gcaggttgag accttcgatg caggcggcgt
    45481 ggaactcacg ctggtcctgg gccagcgcta ccgcaagagc gcgccgctgt tcgtcgacct
    45541 gctgcgcacg caattggcca gttacgcgga cctgttcggc ggaccgccgt tgtcgaagcg
    45601 ctacctcgtc atcatcaacg agcacgagag tggcgatggc ggtgcgttcg ccagcagctt
    45661 cagccagttc gtccagggcg atgcggatgc acgcaaccgg gtgatctggg gttacgtgat
    45721 ggcccacgag ctgctgcatt tctggaacgg cctcagcatc gtccccgccg accatcgcga
    45781 ggaatggttc aaggaaggcg tgaccgacta cctcaccatc gtgaccctgg cccgcaatgg
    45841 ctggctggac gaaaacctgc tgttcaagcg cctggagaac gtgccgcgcc gatacctgat
    45901 cgcgcgttac gcgcagcacc tgcagatgag cgtgcgggat gcgggcaagg acaagcaacc
    45961 caaccggcaa ctggtctacg gcggcggtag cctggccgcg ctggcgctgg acgttgccct
    46021 gcgtgagcgc agcggcgaca aggtcggcct gccggagttc atgcgcgcgc tgcatgcgga
    46081 gttcgccaag cccggcaagg cctatgcgct cgcggacctt gaacgcattg cggggtcact
    46141 gacaggcagc gacttctccg gtttcttcgc cgaagcggtg ggcaatacgt cctatttcga
    46201 catccgcccg agctatgccg cggtgggcct gcgcatggac agcttcgtcg aggagatgtt
    46261 catcagtcgc gagccggcgg cgacgccggt ccagcaggct cgcttcaggg ccgtgttcgg
    46321 tacgccggga gcgccatgcc cacgccagta gccggcatac aatggcggga tgtggaccgc
    46381 catcacccgt gaagtcagcc cggcgctcgc cgattgccag ttgtccttcg tcgagcgcga
    46441 cgccatcgac gtcgagcgcg cacgcgcgca gcacgcggcc tattgcgccg cactcgaggg
    46501 gttgggttgc cgggtgctgg aattgccagc cgaggcggcg tggccggatt cggtgttcgt
    46561 cgaggacgtg gcgctggcgt tcgacgaact cgccgttgcc acccgacccg gggcggaatc
    46621 ccgccgcgac gaaggcagcg cggtgctgga gttgctcggc agcgttcgcc cgctgtcgcg
    46681 gatcgaagct cccgggacgc tcgacggtgg cgacgtgctg cggatcggca agcgcgtgtt
    46741 cgtcggcatc tctgcgcgca gcaatgccgc cggccacgag cagttgcgcg cattgctcgc
    46801 accgcatggc tacgccgtgg cgtcggtggc gacgcgcggc tgcctgcacc tgaagtcggc
    46861 ggtcacccag gtcggcgaag cgacggtgct ggtcaatccg gcctgggtcg atgtcgccac
    46921 gttcgccgat tacgccatca tcgaggtcga tccagccgag cagcacgccg ccaacgccgt
    46981 gcgggtgggc gatgcgctgc tgtatccgga gggctttccg cgcacgctgg cacgcctgcg
    47041 cagcgccggc atcgaggtcg tcacggtgga tgtctcggaa ctgcagaagg ccgaaggcgc
    47101 ggtgacctgc tgcagcatcc tgctgcgcca tgccgcgggc caccgcgaag tcgcggcgtg
    47161 atcctcaaca tcgccgccta cctgttcgtc gccatcgacg acgccgatgc gctggccgta
    47221 cgcctgcgcg aacgcgccga agccgatgcc ctgcgcggca gcgtgctggt caccccggaa
    47281 ggcgtcaacc tgttcctggc cggtgatgcc gatgtgttgc gccgcttcct cgcctggctg
    47341 cgcgaggacc cgcgcttcgc atccctgcag gccaaggaga gctggagcga gacggtgcct
    47401 ttcgcgcggc tgaaggtgaa gcgcaaggag gaggtcatcg cgttccggcg cgagcatgcg
    47461 tcgccgctgg acaccggcga gcgagcgccc gcggtcgcgc cagcgacgct cgcccgctgg
    47521 atcgaacagg gccacgacga tgacggcaag cggctggtat tgctggatac gcgcaaccgc
    47581 gaggaattcg gcttcggcag cttcgcaggc gcactgaccc tgcccatcga caatttcacc
    47641 gacctgcccg acgcactggc gccgcttcgc ggcgcattgg ccgatgccac cgtggtcagt
    47701 ttctgcaccg ggggtatccg ttgcgagaag gccgcgctgt ggctgcgcca tgacggcatg
    47761 gacaacctgc tgcaactgga cggcggcatt ctcgggtatt tcgaggcggt gggcggcgtg
    47821 ggctatgacg gacgctgctt cgtgttcgac gaacgcgtcg cgcttgatcc gcacttgcag
    47881 ccactggtgg acggaatggc aacgaaggac gcagccgcat gagctggatc gggttcgtgg
    47941 tgctgatcgt ggcgatctac gccgccatca aggtggtcgg cgcgctgttc aagatcgcgc
    48001 tctgggtggc catcctcggg ttcgcgtggt ggtactttgg gccgaggatc ggcatcccgt
    48061 tcccgttctg acgctttgct tgtcattccc gccttcgcgg ggatcacgag aaaacgctac
    48121 ccacacagcg cctgtcccgc cgcatgaccg ctggcccagg cccactggaa gttgtagccg
    48181 ccaagccagc cggtgacatc gacgacttcg ccgatgaagt acaggccggg cacgtgtttc
    48241 gacatcatcg tgctggatga caggccgtcg gtatcgacgc cgccaagggt gacttccgcg
    48301 gtgcgataac cctcggtgcc gctggcgacg agtggccacg acgacagcag gtcggcgatg
    48361 ccgcgcagtt ccggcgcgtt gtattgccgc aacggcttgc tggtgatcca gtgctcgcac
    48421 aggcgttggg cgaagcgctt cggcaggact tcgcccagca gggttttcag ctcggcgtcg
    48481 cggcggctgg cctgccagtt gcgcaacagg gcatcggcat cggtgcccgg cagcaggtcc
    48541 aggcgcaggt cgtcgcccgg ttgccagtag ctggagatct gcaggatcga cggtccgctg
    48601 atcccacggt gggtgaccag catgaagttg cggaagctcg ccccgttgca gcgtgcctcg
    48661 accggcagcg agacacccgc caggtcctgc aggcgttcgg cgtgcttgcc ggtcagggtc
    48721 agcggcacca ggccggcgcg ggtcggcagc acctcgtggc cgaactgctt cgccagcgcg
    48781 tagccgaaac cgctcgcgcc catgctcggg atcgacaggc ccccgctggc gacgatcact
    48841 gcgggcgcgg cgaaactccc ctgcgacgtg cgcaggcgga agccgccgtc atccaggtgt
    48901 tccacgcgct cgatcgtgca ttgcgtgcgg acctcgacgc ctcctgccgt gcattcgtcg
    48961 agcagcatcc tgacgatgag tttcgaggac tcgtcgcaga acaactggcc gagttccttc
    49021 tcgtggtagg cgatgcggtg ccgctcgacc aggtcgatga actgccaggg cgtgtagcgc
    49081 gccagcgccg acttgcagaa gtgcgggttc gccgacaggt agttcgcggg cgtggtgccg
    49141 gtgttggtga aattgcagcg ccccccaccg gacatcagga tcttcttgcc gaccttgttg
    49201 gcgtgatcga ccaccagcac gcgcaagccg cgcttgccgg cggtgatcgc tgccatcagg
    49261 ccggcagcgc cggcgccgac gacaagtgcg tcgtagctgc gctcgctcac gcccactcgc
    49321 gcgagggcag ggtgaagcgg cgctgcgggc gtccgaccag gtcgaggaaa ttcccgaaca
    49381 cggcatccgc ctgttgctgc atggccgcaa tgtgcagccg tgtctgcgcg cggatcgcgt
    49441 ccgcgtcgtg cggcaggccg gaatcggcga gttcctctcg gtaggcagga ttggccagcc
    49501 agcgttcgac caggcgctcg tccatttcca ggtggaactg gaagccgtag gcgttgtcgc
    49561 cgtggcggaa ggcctgctgc tcgcattgcg ggctgcgcgc gaggtgcacg gcaccctccg
    49621 ggatgtcgaa tcggcagcca tgccactgga acaccggcgt gtccgcctgc agcggcgcga
    49681 gtaccggatc gctcgttcct tccgtcgttt tccgcaacgg ataccagccg atttccggca
    49741 cttcgttgcg gcgcaccggc gcgcccagca catgcgccag cagttgcgcg cccaggcaga
    49801 tccccagcac cggcttgccc tgttccagca tccgctcgat ggcgatcatc tcgttgcgca
    49861 ggtgcgggcg ccgtgggtgg tcttcgacat tcatcggccc gcccagcacg accaggccgc
    49921 gataacggtc gacgttgagc tgggcatccg ggtggcgctc gaaattcttg aagcggatcc
    49981 ggtgcccgcg cttgcggatc aacggatcga gcgtgcccag cggttcggcg gcgacgtgct
    50041 ggaagacgag gaggcgcggc atgcggcgga tgatggcaaa gaccgcgacc tcatcgccaa
    50101 tcgcgatggg tcatgcgctt atcgtcactc agaagcgtgg gccacgcggc ttgaagccac
    50161 ccggcttgcg tggtccgccg ccggggcggt cgccgcgcgg tgggccgaag ctgcgcttgg
    50221 ccttgggcgc gtcgatgtcc gccgactcgg cggggcgcat ccgcaggggg cggctggcga
    50281 cccgcacggt ctgcatgtgc gcgagcaggt cggcgggcat gccgtcgggc aggtcgacca
    50341 gggtgaagtc gtcgcggatg tcgatgcggc cgatgaaacg cgcctccagg tcggcctcgt
    50401 tggcgatcgc gccgacgatg ttgcccggct gcacgccgtg ctggtagccg acctcgatgc
    50461 ggtaggtctt catgccgacc tcgggcgcac cgtgctctcg cggtgcgtgt tcacgcgggg
    50521 cacgctcgcg cacggggcgc tcgccccgct ccgggcgctc gctgcgttca cgtggctcac
    50581 gcacctcgaa cgcgcgttcg ggcgcggcgg gcttggcgag cagcagcggg gtcttgccct
    50641 gcaccagctg cgccagggcg gcggcgatct ccaccgcggg cacgttcttc tcgcgctcgt
    50701 agcgttccac caggtcgcgg aagacggaca ggtcggcgct gccgagcgcg ccctcgatct
    50761 tgtcgaggaa cttggccacc cggcgctcgt tgacggcatc gacgctcggc aggttcatct
    50821 gttcgatctt ctgccgggtc gcgcgttcga tcgcgcccag catgccgcgc tcgcgcgggg
    50881 ccacgaacag gatcgcctcg cccttgcggc cggcgcggcc ggtgcggccg atgcggtgga
    50941 cgtagctttc ggtgtcgtag gggatgtcgt agttcagcac gtggctgatg cggtccacgt
    51001 ccagcccgcg cgcggccacg tcggtggcca ccaggatgtc gatctggccg tccttgaggc
    51061 gctggatggt gcgctcgcgc atcttctggt cgacgtcgcc attgatcgcg gcggcggaaa
    51121 aaccgcgtgc ggcgagtttc tcggccagct cgtcggtgcc cagcttggtc cgcgcgaaca
    51181 cgatcatcgc ctcgaacggc tcggcctcca tgatccgggt cagcgcgtcc agcttgttga
    51241 tgccgctgac catccagaag cgctggcgga tgttctccgc ggtcgaggtc agcgacttga
    51301 tcgcgatctc caccggatcc ttcaggtagg tctgggcgat gcgcttgatc tgcgccggca
    51361 tggtcgccga gaacagcgcc acctggcggg tttccggggt cttcttcagc actgcctcga
    51421 catcgtcgat gaagcccatc cgcagcatct cgtcggcttc gtccagtacc agcatgcgca
    51481 gcgcggacag gtccagcgag ccgcgctcca ggtggtcgat cacccggccg ggcgtgccga
    51541 ccacgacatg caccccgcgc ttcagcgcgc tgagctgcgg ctggtagctc tggccgccgt
    51601 agatcggcag cacgtggaag ccggggatgt gggtcgcgta cttctggaag gcctcggcga
    51661 cctggatggc cagttcgcgg gtgggcgcca gcaccagcac ctgcggcttg ctggcggcgg
    51721 cgtcgagctt ggccagtgcg ggcaaggcga acgcggcggt cttgccggtg cccgtctggg
    51781 cctggccgat catgtcgcgc ccggccagca gcggcgggat ggtggcggcc tggatcgggc
    51841 tgggggattc gtagccgacg gtgcgcaggg cctgcagcag ggtctcgggc aggccgaggt
    51901 cggtgaattt gatgtcgggg gtggtttcgg cgctcatatg cgtcccatgt gactcttgga
    51961 ttgggcgtga ccgccgggat tgaccgcaca gtgtacgccc ggtggcctga gcccaggctc
    52021 cagtgccggg ccagctacca tgatccatgc acttcccgac gttcgacaac cgcttactgc
    52081 gtgccttgcc gggggacccg gagacgcggg ttttcccgcg acaggtccag gcggcgctct
    52141 ggtcttcggt ggagccaacc ccggtcgatg caccgcgcct gctggcgcat tcgcgggaca
    52201 tggccgccga gctgggcttc agcgaggccg acgtggcctc agccgaattc gccggcgtgt
    52261 tcggcgggaa tgcgctgcta cccggcatgc agccgtttgc gaccaactac ggcggccacc
    52321 agttcgggca ctgggcaggg cagttgggcg atggacgcgc gatcagcctg ggcgaggctg
    52381 tgttggcgga tggccggcgt cgcgagttgc aactgaaggg tgcagggccg actccgtatt
    52441 cgcgcagcgc cgacggtcgc gcggtgctgc gctcgtcgat ccgcgaattc ctctgcagcg
    52501 aggcgatgca ccacctcggc atccccacca cccgcgcgtt gagcctggtc gccaccggcg
    52561 agggcgtggt ccgcgacatg ttctacgacg gccgtccgga ggttgaagcg ggcgccatcg
    52621 tgtgccgggt cgcaccctcg ttcctgcgtt tcggccattt cgagttgccg gcgatgcgcg
    52681 gcgacatcga cctgctgcgg aagctggccg atttctgcat cgcccgcgat ttcccgcacc
    52741 tgcaggggca gggcgccctt cgacaggctc agggcgaacg gatctacgcc gactggttcg
    52801 ccgaggtctg cgagcgcacc gcgcgactgg tcgccggctg gatgcgggtc ggtttcgtcc
    52861 atggcgtgct gaacaccgac aacatgtcga tcctcggcct gaccatcgac tacggcccgt
    52921 acggctggat cgacgcctac gaccccgact ggacgccgaa caccaccgac gccgccggcc
    52981 ggcgttaccg cttcggctgg cagccgcggg tggcgtactg gaacctgggc cagctggcgc
    53041 gtgcgttatc cccgctgttc gcggatcccg cgcctctgca cgctgggctg gaacgcttcg
    53101 cgactgccta caccgcagcg gaccgcgaca acaccgcgcg caagctcggg ttggccgagt
    53161 gtcgcgacga ggacatcgac ctgctgcggg acctgcacgg cctgatgcaa tcccacgaag
    53221 tcgacatgac gctctggttc cgcgcgctgg ccgatctgga tccggcacgg ccttcgctga
    53281 ctccgctgga agacgccttc tacgacgccg gcaagcgcgt ggcgggcgag gctgcgttca
    53341 acgactggct ggcgcgttac gccgcccgtc tgaacgaaga tccgcttgtg ggcgatgagc
    53401 gccgcgcccg catgcacgcg gctaatccga actacgtgct gcgcaactgg ctggcccagg
    53461 aggccatcga tcgcgccgat gccggcgaca tggccggcat cggcgacttg ctggaggtga
    53521 tgcgccggcc atatgacgac cagccgggca gggaacgatt tgccggaaaa cgcccggatt
    53581 gggcgaagga caaggcgggt tgctcgatgc tgtcctgcag ctcctgagcc cccgcctgtt
    53641 gcctgggctt acttgccctt cttttccttc ttcgccgcgc gcttttcctt cagcgacttc
    53701 tccggcttct tcttttcggt ctttttctgg tccatgccct tgctcatggc gatctccggt
    53761 cgagtgggtg ggtgctgctg ggcgcaagcc taccgcgccc gcgcccgcgt gcacacgggc
    53821 gcgatcaagc ccgcgggtgc gcgacggcgc cggcacgtac aatgggcgga tgcctttgat
    53881 cactttgcag ggcctcgact acagcgtcgg tggcccgttg ttgctggaac atgtcgacct
    53941 ggccatcgaa gcgggcgagc gcatcgccct gatcggccgc aacggcgccg gcaagtcgac
    54001 cctgatgaaa ttgctggccg gcgagctgaa gccggacgat ggcgaagtcc gcgtcgaagg
    54061 cggtcgccgg atcacccggc ttgagcagga agtgccgctg gatgcggccg gcagcgtgtt
    54121 cgacgtggtc gccgctggcc tgggcgatgt cggtgcccaa ctcgcgcact accaccacct
    54181 gatccatgcc gagcccatcg acatggacgc gctggccgac gtgcaggcga agatcgaaag
    54241 cgccaacggc tggtcgctgg accagcgggt catcgagacg ctggaacggc tcgggctgga
    54301 tggcgatgcc gatttcgccg cgctctcggg cgggatgaag cggcgcgtgt tgctggcgcg
    54361 cgcgctggtc tcgcaacccg acctgctgtt gctcgacgag ccgaccaacc acctggacat
    54421 caccgcgatc gactggctgg agggcttcct caagcagtgg agcggggcgc tggtgttcgt
    54481 cacccacgac cgacgcttcc tgcgcgcgct ggctacccgc atcgtcgaga tcgaccgcgg
    54541 ccaggtcacc agttggcctg gcgactggga caactaccag cgccgcaagg aagagcggct
    54601 caacgccgag gcgcaggaaa atgcccgctt cgacaagttg ctggcgcagg aggaagtgtg
    54661 gatccgccag ggcatcaagg cccgccggac ccgcgacgaa ggccgcgtgc gccgcctgaa
    54721 ggcgatgcgc gaggaccgcg cgcagcggcg tgcgcagacc ggcaacgtga agatggccac
    54781 cgcgcaggcg ggggaatccg gcaagaaggt gatcgaggcg aagggcgtga gcttcgcgca
    54841 tggcgacaac gtcctggtgc gcgatttcag caccgtgatc atgcgtggcg accgcatcgg
    54901 cctggtcggc ccgaacggca gcggcaagac caccctgctc aagctgctgc tgggcagcct
    54961 gcagccgcag gccggcgaga tccgcagcgg cacccagcta cagatcgcct atttcgacca
    55021 gtaccgcgcc accctgcgcg aggactggaa cgccatcgaa aatgtcgccg aggggcgtga
    55081 gttcgtggag gtcggcggca agcagaagca catcatcggc tacctgcagg acttcctgtt
    55141 caccccggaa cgcgcccgcg cgccgatcac ccgcctgtcc ggcggcgagc gcaaccgcct
    55201 gctgctggcg cgattgttcg cccaaccgtc caacctgctg gtgatggacg aacccaccaa
    55261 cgacctcgac gtcgaaaccc tggagttgct ggaggaactg ctgggcgact acccgggcac
    55321 gctgttgctg gtcagccacg accgcgactt cctggacaac gtggtgacct cgaccatcgt
    55381 catggagggc gacggaagga tcggcgagtt cgtcggtggc tacagcgact ggctgcggca
    55441 ggcggcatcg tccgcggagc ggcaggcgca ggcgcctgcc aggtccacgc cccctgctgc
    55501 tgcaccggcg gtttctacac agccgaaggc caagcgcagc ttcaaggaac tcaaggagct
    55561 ggagcaactg cccttgcgca tcgaggcgct ggaaacgcag ctggcggcga tgaccgcgcg
    55621 catggccgag cccggctact accagcgcga tgccgcccag gtcaccgcgg acaatgctgc
    55681 gatggcggca tcgcaggccg aactcgatgc cgcctacctg cgctgggcag cgctcgaagg
    55741 cggttgagcc gtgatgctgg cgacgttttc acccgggtga tgcaaccggg tatggccgcc
    55801 gccaagcctg cccggctaca ataatgcgga tatagcgagc ggccttggtg ccggctggcc
    55861 ctcccaagaa gaacaatcga ggcacgcgtg ctggccgaat acctgcccac cctgctgttc
    55921 ctgatcgtcg ccaccggcat cggcatcgtc ctgatcgtgg tcggcaacgt gttggggccg
    55981 aagcgcccga gcgccgacaa gctcgatccg tacgaatgcg ggttcagcgc gttcgagaac
    56041 gcacgcatgc cgttcgacgt gcgctattac ctgatcgcga tcctgttcat cgtgttcgac
    56101 ctggaaatcg ccttcgtctt tccatgggcc ctggtgttcc gcgagctcgg ggtgttcggc
    56161 ctggtcgaaa tgggcgtgtt cctgggcctg ctggtgatcg gcttcgtgta cgtgtggaag
    56221 cgcggcgcgc tggagtggga atgagcaata tcgtccagaa agtcctggaa atcgccagca
    56281 acccgctgcc cgaagggcgg ctggacgaca tcctgcgccc ggaaggcgac aacccgctgc
    56341 tgcagaacgg gttcgtcacc accagcatgg atgcgctgtg gaactgggca cgcaccggct
    56401 caatgtggcc gatgaccttc ggcctggcct gctgcgcggt cgagatgatg catgccggcg
    56461 cggcgcgcct ggacctggac cggtacggcg tggtgttccg cccgtcgccg cgccagtccg
    56521 acgtgatgat cgtggccggc accctgtgca acaagatggc cccggcgctg cgcaaggtct
    56581 acgaccagat gcccgacccg aagtgggtca tctcgatggg cagctgcgcc aacggcggcg
    56641 gctattacca ctattcgtac tccgtggtcc gtggctgcga ccgcatcgtc ccggtcgacg
    56701 tgtatgtccc cggttgcccg ccgaccgccg aggcgctggt ctacggcatc ctgcagctgc
    56761 agaagaagat ccgccggatg gagcagaacc cgttctccgg cgccgcgaag cccgagggcg
    56821 cgcgcgcatg agccttctcc atgagttggt cgcccgcttt ggcgaagcct cggtgcaggt
    56881 cgcgcagcca cgcggcgaaa tcaccctcga ggtcggcgcc ggcgaatggc tggctgcctg
    56941 caccgccttg cgcgatgcgt tcggcttcga caccttcatc gacctctgcg gcgtcgacta
    57001 cctgagctac ggcgacgacg agtgggacac cgatggcgtg tccgcgcagg gcttcagccg
    57061 gggcgtggaa gggcagggcc cgggtcgctt caagtggacc gagcagccca gccaccaggt
    57121 cgccgatccg cagggaatcg cgccggattc gctggaaggc aagcgctatg ccgtggtcgc
    57181 ccacctgctg tcggtgcgca acaacagccg cgtgcgcgtg cgttgcttcg ccgccgacaa
    57241 cagcctgccg gtggtgcctt cgctggtggg cgtctgggca ggcgtgaact ggttcgagcg
    57301 cgaagcgttc gacctgtacg gcatcatctt cgaagggcat cccgaccttg ccggatcctc
    57361 accgactacg gcttcgtcgg tcatcccttc cgcaaggact tcccgctgat cggcaatgtc
    57421 gaagtccgct acgacgagg
//
LOCUS       DAOOCX010000002        51740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_437_length_51740_cov_8.202612, whole genome shotgun sequence.
ACCESSION   DAOOCX010000002 DAOOCX010000000
VERSION     DAOOCX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 51740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51740
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_437_length_51740_cov_8.202612"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1193
                     /locus_tag="PK399_00305"
     CDS             <1..>1193
                     /locus_tag="PK399_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPW11601.1"
                     /translation="FQKEQGIDLRKDPLALQRLKDAAERAKIELSSSQQTDVNLPYVT
                     ADATGPKHLNIKLTRSKLEALVDDLVKKTIEPCRIALNDAGLRASDIGEVILVGGQTR
                     MPRVQQAVTEFFGKEPKKDVNPDEAVAIGAAIQGGVLQGDVKDVLLLDVTPLSLGIET
                     LGGVFTKIIEKNTTIPTKASQTFSTAEDNQSAVTVHVLQGEREQARFNKSLAKFDLTG
                     IDAAPRGMPQVEVSFDIDANGILHVNAKDKKTGKEQKVEIKAGSGLSDDEIQRMVQDA
                     EANREEDKRFHELVTARNQADGLIHATRTAIKDNGDKVSGDLIGRAEGAIAELETAMK
                     GDDKGQIDAKAKALEEAGQALFAAAAAAARPGAGGGDAGQAPGAGDDVVDAEFTEVKD
                     DNKGA"
     assembly_gap    1194..1293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1428..2561
                     /gene="dnaJ"
                     /locus_tag="PK399_00310"
     CDS             1428..2561
                     /gene="dnaJ"
                     /locus_tag="PK399_00310"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPW11602.1"
                     /translation="MSKRDYYDVLGVARNASDDELKKAYRRSAMKHHPDRNPGDASAE
                     AAFKECKEAYEVLSDGGKRRMYDQHGHAAFEHGMGGGNAGPGFGGADMGDIFGDIFGN
                     IFGGGARQQGPRRGADIGYVMELDLEEAVAGVEKRIEIPTLANCEPCKGSGSADGKVE
                     TCATCHGRGQVRMQRGPFAMQAPCPHCAGTGKTIPNPCPQCDGSGRIEEEKALSVKIP
                     AGVDNGDRIRLAGEGEAGPAGAPPGDLYVEVRVRPHAIFARDGDDLHCEVPIRISQAA
                     LGDSVRVPTLGGEVELRIPVETQTGKVFRLRDRGVKSVRSRHPGDLYCKVVVETPVNL
                     TNEQRELLEQFEATFTGENARRHSPKSSTFLDGVKGFWDRMVS"
     gene            2678..4897
                     /locus_tag="PK399_00315"
     CDS             2678..4897
                     /locus_tag="PK399_00315"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HPW11603.1"
                     /translation="MSFRSPTEIPPQTPPDRGRLLDSLQKQMLERVLPALDRALGKVD
                     DYLFDRSQQGDEEFGLMALRELRRQRGQIARRYEDVVQAGFRNLRSASEWFPADGRSS
                     DLSLLSEQELEEQLAVEQLADALVRVHGPGVEMSGKRIAHIAGREDLQAQDNPLAPAF
                     LAAALRTALAPSELEAGVRIVVFKFFERELGAVLGAAYERANTLMIAAGVLPELRATV
                     KATAQPQAPASSPDPHAPQAPSGQVQLPAQVSAVDQAMFGNLLGLLQGWRTAMGGNRA
                     DAEGGPLQPGAQQAPVTKLGSSELMSILSLMQQDMPNAPLPPREAGGDHRTLAEQLRK
                     EVAQNARKLGVAGDNLHLDGLDEDAVDLVALLFDVLLDGPQFDANIRRKIGRMLVPYV
                     KVAVKDRRMFLFKEHPARRLLNTVAEACEGNHGEAPQEQALLNRVDYTIDRLVAEFNE
                     DVAIFETLEQELRSYMAQHRKRFELAEKRATEAQRGRERLDLARSAVAAELMQHRGER
                     ELPAVMDEFIGRHVSHHLTQVMLRDGHGSPRHEQAIQAVDRLLLAFDYAEVRSPLEEL
                     PTLPRAQFEAILSSSGCTGDSAQLAIDSLQDALVRLSAGERAADIPGHMPEQVILPEP
                     KAVEPEPQLEVVAGNDHLDYDAAMLDRMRKLQVGSWLQLATSADRVEPSKVSWISPIS
                     SRLLLVNRRGIRVLVASAEELAAMAKLGKVSLREGDSVFDNAMHQVADRLKNAASQL"
     gene            4950..5660
                     /locus_tag="PK399_00320"
     CDS             4950..5660
                     /locus_tag="PK399_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPW11604.1"
                     /translation="MTNPVRLLVHGANGRMGQSLQRLCREENAGCTVVAAVSRTVDAR
                     VVDGIPQFASSELAGVPAFDVAVDFSLPAGFDAILGLCVARGAGLVSGTTGLSVSQEA
                     ALADAATRIPLAWASNFSLGVAVLHDLVERAARALPGWDCDIVESHHTRKRDAPSGTA
                     LTLGDSAGQGGATPHYASIRAGDIVGEHLVQFTTAGERIELIHRAGNRDIFARGALHV
                     AARLKGRAAGRYRVADLL"
     gene            5835..6980
                     /gene="carA"
                     /locus_tag="PK399_00325"
     CDS             5835..6980
                     /gene="carA"
                     /locus_tag="PK399_00325"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017115232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPW11605.1"
                     /translation="MNIPAILVLEDGTVFEGISVGAAGLTVGEVVFNTAMTGYQEILT
                     DPSYARQLVTLTYPHIGNTGCTAQDDEADRVWAAGLIVRDVPRRASSWRSEIALPEWL
                     RARGIVAISGIDTRKLTRILRDRGSQNGALMAGAAPDAQLDVDKAVEAARKFPGLKGM
                     DLAKVVSTQANYTWRDGQLDLDRNAFVRADSRFKVVAYDFGVKHNILRMLAERGCDVT
                     VVPAQTPAADVLAMQPDGVFLSNGPGDPAPCDYAIAAIRDFIAAKVPTFGICLGHQLL
                     ALAAGARTLKMPHGHHGANHPVQDLTGAGGGKVMITSQNHGFAVDETSLPANVRVTHR
                     SLFDGSNQGIELTDAPAFSFQGHPEASPGPHDVAPLFDRFVALMETH"
     gene            6980..10219
                     /gene="carB"
                     /locus_tag="PK399_00330"
     CDS             6980..10219
                     /gene="carB"
                     /locus_tag="PK399_00330"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002806440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPW11606.1"
                     /translation="MPKRTDLKTILIIGAGPIVIGQACEFDYSGAQACKALRDEGYRV
                     VLVNSNPATIMTDPEMADAVYIEPINWRMVEKIIAKERPDALLPTMGGQTALNCALDL
                     ADNGVLEKYGVELIGAKREAIMMAEDRELFRVAMKEIGLECPKAEVARTLEQALDIQT
                     RVGYPTIIRPSFTLGGSGGGIAYNREELIEIVNRGLELSPTSEVLVEESVLGWKEFEM
                     EVVRDTADNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQRLRDASIAVLRKIG
                     VDTGGSNVQFGINAQTGRVVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNEITGGKTPASFEPAIDYVVTKIPRFAFEKFPAADARLTTQMKSVGEVMAIGRCFQ
                     ESLQKALRGLETGKVGLDPTGLDLVDEDDLVKLRRELKEPGPERIFFLADAFRAGMSV
                     EEVHALSFVDPWFLDQIEELIDAEVDVVHRGLDGLDKRRLRALKRMGFSDARIAQLAR
                     TSESAVRALRKAFHVRPVYKRVDSCAAEFATTTAYLYSTYEDECEAQPSDRRKVMVLG
                     GGPNRIGQGIEFDYCCVHAALALREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLE
                     DVLEIVELEKPVGVIVQYGGQTPLKLAKALEANGVPIIGTSPDSIDLAEDRERFQKLV
                     DSLGLKQPPNRTARSAEEALAHAREIGYPLVVRPSYVLGGRAMEVVHADADLARYMRE
                     AVKVSHDSPVLLDRFLDNAVEVDIDVIADREGNVLVGGVMEHIEEAGVHSGDSSCSLP
                     PYSLSADTQARLRVQVVALAKALNVIGLMNTQFAVQTNGDGEDTIFLLEVNPRASRTV
                     PFVSKAIGQPLAKIAARCMAGMTLAEQGATKEIIPGYYSVKEAVFPFAKFQNVDPILG
                     PEMRSTGEVMGVGRSFEAAFARAEEAANIKAPQPGKAFISVRDPDKARVLPVARYMLE
                     RGFSLVATQGTADVLAAAGIECARVNKVIEGRPHIVDLIKNGEICYIINTTEGRQAIA
                     DSFSIRREALQQRVTYSTTVSGASALLHSLDHRGAGDVLSLQELHKELA"
     gene            10219..10695
                     /gene="greA"
                     /locus_tag="PK399_00335"
     CDS             10219..10695
                     /gene="greA"
                     /locus_tag="PK399_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009600880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPW11607.1"
                     /translation="MHAPITAKGALRLRAELEELKSVKRPEVIAAIAEARAHGDLKEN
                     AEYHAAREQQGFIEGRIKHLEGELSHAQVIDIANLSVGDKVVFGATVELADVDTDEQK
                     TYQIVGDLEADIKQGLIAISSPVARAMIGKHEGDAITIEAPGGIREYEIISVKYLG"
     gene            10700..11632
                     /locus_tag="PK399_00340"
     CDS             10700..11632
                     /locus_tag="PK399_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HPW11608.1"
                     /translation="MPRLTLLLPATTRFAGVALPQALAAALGRADRAMHEAGEDAQLA
                     RHFRLLPNRWAHAALTRVVDGGQEDARLSGWLRADPAYIRPDINGARLLATGDGLGID
                     AADVEAVLPALRPLFGDVGFGLDAPHPGRWYLRLPKEAALPAFASPTQALGDDVFEHI
                     PAAPEARRWRLLASEAQVVLHNHPRNATRAAAGKPPINALWFWGGGVLPDHVASASPT
                     LYSDDPLLHGLAKLGTLDAMPLDAAKESEDDGLIDLRAVRDPRADVARWLSPAAQAAS
                     RGEVVFDFADGMVFTVAPGQRWRFWRKPAARIDA"
     gene            11629..13329
                     /gene="recJ"
                     /locus_tag="PK399_00345"
     CDS             11629..13329
                     /gene="recJ"
                     /locus_tag="PK399_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPW11609.1"
                     /translation="MSATRRIVRRDAVEARGDWPASWPPLLRRVHAARGSDAREAMPR
                     LADLLPFTGLAGIEHAVELLRDAVHRDARILVVGDFDCDGATACAVGVRGLRMLGANH
                     VMHAVPNRIVHGYGMTPGLVEELAALHPDLVVTVDHGIACHAGIDAAKARGWQVLVTD
                     HHLPGPTLPSADAIVNPNQPGDTFASKAMAGVGVIFYLLLALRARLGASADLASLLDL
                     VAVGTVADMVALDANNRALVGAGLRRLRAGQGCAGLRALIDVSGRREATLSTADIGFA
                     IGPRINAAGRLEDMGIGIECLLSDDVGHARQLASVLHGINEERRAVQADMQDVAEARL
                     DGVGQDAPAACLFDEDWHPGVVGLVASKLKERLHRPAIAFAPVEPGGAALRGSARSIP
                     GFHVRDALALVDARHPGLIDRFGGHAMAAGLSLQVEAFPAFCDAFLQVASDWLTDDLL
                     TDVLRSDGALADADFHIDTALALRDGGHWGQGYAEPLFDGEFDVLAWRIVGERHLKLE
                     LGAGGKRLNAIHFGGWDGDAPPSRLHIAYRLVPDDYRGNGAIQLLVVHREPASSGLRA
                     "
     gene            complement(13274..14461)
                     /locus_tag="PK399_00350"
     CDS             complement(13274..14461)
                     /locus_tag="PK399_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPW11610.1"
                     /translation="MQRRFDLDWVRILAFALLVPYHVGMYYVSWDWHVKSPELVPALE
                     PFMILSAPWRLALLFLVSGVATAHLRAKLPVGFLRQRSWRLLLPLLFGMYVVVPPQAY
                     LELVEKVPGGYHAGYLGFMDDYLKGGDLYCMAPGDCVDSPTWNHLWFVAYLWVYSAVL
                     WATCRLLPPSWRAKGAAFVRRACSGHGILWWPMLALVAARVLVYPHAPSTHDLVDDAY
                     NHLQYGLVFALGYLLTSTDAAWEPLRRHRHRMLLLAAASYLSMLAAWLQFDDADPIPT
                     AWMSLIWTCWGMMEWAAIAAILGYARNWNPQDSPLLRTLTVAVFPFYILHQTVIVLLA
                     HNLKPLHLPPVLEGPLLVVATFGLCLLGYAVIRRIAWLRPLFGLKREVRTTPVHGARP
                     ATG"
     gene            14616..15494
                     /locus_tag="PK399_00355"
     CDS             14616..15494
                     /locus_tag="PK399_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPW11611.1"
                     /translation="MDAAVPTPFQRYQPWRRSVEIGFWVVSYLLSAIGNSVTTNMDVS
                     RLALGFASWQPVAWESSSAIAALALVPAVAWFTRRCPLHLDTWRRMLPAHLLASVVWS
                     ALHVIAMVAIRKAVYATQGQHYDFGTWWWEFGYEYLKDIRSYAGVVATIEVYRFVLRR
                     WQGEASLLDVPDDGPPVESVDRPERFLVRKLGREFLVAANDIEWMQASGNYVNLRMRG
                     HDYPLRSTIGGIEGKLDPERFVRVHRSYIVNLDQVGSIEPLETGDARVHMKDGSNLPC
                     SRTYRGGLRQRVGLDA"
     gene            15558..18494
                     /locus_tag="PK399_00360"
     CDS             15558..18494
                     /locus_tag="PK399_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HPW11612.1"
                     /translation="MKRLLLTLAAGVAAIGITLDSPAQHVRPQPQPVIPAWIEVPGAE
                     TPVTLERAEVEVGTAGGLARTSVVLTLRNPNARILEGTLQFPLQPGQQVTAFALDVGG
                     VMRDAVPVPKQQGRQVFESIERRNVDPALLEQTAGNHFRLRVYPLPARGTRQVRLVLD
                     EPMRRDGDEWRLALPAGLLSAASAVSLRVQDAEKAPILDGTFSRPRWRRDGDGQRTEL
                     QRGDAGSVAGLSLRFPTSTSVRAYVGVQGDAHYVLAEMPVAATSKPRIIPKHVGLLWD
                     ASASGRKRDIAGELELLDRYFKAMGDGRVRLRVLRDRGTEAGSFPVRGGDWSELRKAL
                     SAVIYDGATDLADWRPTPGIGEYLLVSDGLQNYGTQAIPTIQAGQRLYALASTAADAT
                     RLAAIAEARDGRLVAWQGRAGIDAAARALLVDGTHLVALEGEGVDQLVAQSRWVDGDI
                     LRVAGRLREPKGRIIASVEDANGRRRIEVPVASAGIAHAQVPQAWAAWSVARLSGEPE
                     RNRAAITRLGTEFSLVTAGTSLLVLDDPADYVRYDIPAPPALQAQVVTIRSQQHRERE
                     ASRSEQLDALATEFAGRVAWWERAFPKGARAVAQPRKAEAGAGTLDTVTVTGSTVLGR
                     NAAASMAPPPAPIAEAPAADATTLDTVTVAESGAIHAIDVSSDESTRIAGNVQATIRL
                     QPWEPDSPYARRLRAAPADQLYPLYLDERDSHAASTAFYLDVADLLLQRERRDEALRV
                     LSNLAELELENRHVLRVLGYRLMQAKAYPLAVQVFRQVLEMADEEPQSHRDLALALAA
                     DGERQEAIRHLYDVATREWDGRFAEIELVALNELNQIVATAPAPLETGFIDPRLLRNL
                     PLDLRVALSWDSDNSDMDLWVTDPNGEKCFYGNTLTYQGGLISEDFTGGYGPEEFVLR
                     DAKPGKYRVEAHYFGDRQQVVTGATTLSLRLSTGWGTRQQRDQAVTLRLSGKDESVLV
                     GEFEVR"
     gene            18576..19704
                     /gene="prfB"
                     /locus_tag="PK399_00365"
     CDS             join(18576..18650,18652..19704)
                     /gene="prfB"
                     /locus_tag="PK399_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535374.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPW11613.1"
                     /translation="MIELNPIRARIADLTGRLDALRGYLDYDAKRERLEEVERELENP
                     DIWNDPERAQALGRERSMLDKTVNGIRDLKDGLGGAGELLELAEMEDDEETALAVVAD
                     VDRFEKGVDQLEFQRMFSGKMDSANAFVDIQAGAGGTEAQDWAEILLRMYLRWAENRG
                     WKTELMEVSGGDVAGIKSATFRVEGEYAYGWLKTEIGVHRLVRKSPFDSDNRRHTSFT
                     SVFVSPEVDDDIEIDINPADLRTDVYRSSGAGGQHVNKTESAVRITHIPTNTVVACQN
                     GRSQHQNRDTAMKMLAAKLYELEVQKRNAEKDALEATKSDIGWGSQIRNYVLDQSRIK
                     DLRTGVERSDTQKVLDGDLDEFVEASLKSGLEAGSKRVDAA"
     gene            19754..21274
                     /gene="lysS"
                     /locus_tag="PK399_00370"
     CDS             19754..21274
                     /gene="lysS"
                     /locus_tag="PK399_00370"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPW11614.1"
                     /translation="MTDETQTPQTDENHLIAERRGKLAALRTQGIAFPNDARRADFAG
                     DLQADYADVEAWTAEALEATGRRVAIAGRLLAKRVMGKAAFATLQDMSGRIQLFLQST
                     ALGEAYDAFKGWDIGDIVAVEGGVTRTRTGELSVKVDALRLLVKSLRPLPDKFHGLAD
                     VEQRYRQRYVDLIVNPEARDVFIARSRIVAAIRRWLDARRFLEVETPMMHYIVGGATA
                     KPFTTHHNALDLDLFLRVAPELYLKRLTVGGLERVYEINRNFRNEGVSTRHNPEFTML
                     ELYEAYATYIEVMDLTEGMIRDVAREAMATTVFEWDGNRIDVAPAFRRWKLEEAVREL
                     NPEISVADCRDRDALAAHCARLGVHVKAGYGWGKLLLEIFEKTVEAGLVQPTFITHYP
                     VEVSPLARESDSEPGITDRFELFIGGKELANGFSELNDPEDQAARFRAQVEAKDSGDD
                     EAMHFDADYIRALEVGLPPTGGLGVGIDRLVMLLTGSSSIRDVLLFPYMRPEHATG"
     gene            21441..22499
                     /locus_tag="PK399_00375"
     CDS             21441..22499
                     /locus_tag="PK399_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HPW11615.1"
                     /translation="MDIVIVDDQPSARTMLRHIVQDIGPQLDVHDFGEPDAALEWCNE
                     NRPDLLLLDYRMPGMDGLELAKAFRRPLRNRDVPIVLVTVVGDEPVRQAALDAGVIDF
                     LVKPIRPRELRARCRNLLQLRQQSESVKQRALSLEQRLLASMHEVEERERETLSRLAR
                     AIEFRDAGTSAYLERMAHVAGLVAEAIGLPEEQARTIELAAPLHDIGKIAIPDSVLMK
                     PGPLDDAERRRMQEHPSIGYQLLQDSQNRFIQVGAKIALHHHERYDGTGYPLGLAGEE
                     IPIEARVVTVADVFDALISRRPYKDAWPIERALEFIEGQSGKMLDPVCVRALLDNLPR
                     LHDICERYSQVVPQPGIH"
     gene            22503..24680
                     /locus_tag="PK399_00380"
     CDS             22503..24680
                     /locus_tag="PK399_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008571489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW11616.1"
                     /translation="MDGAARRMLSRLRGRQDSEHAQVFVRIVITTLFAAYLGWEVSGG
                     DGNRALFLTWWILLGEWLLSVVLLAAIVASPGPSDLRRWIGMLADYAAMGGVMHLQGE
                     TAAPLYAVFLWVTIGNGMRYGPGYLFRATALAGLSFGAMMQATPYWQENPYLSWGLLM
                     GLVAVPLYFASLLKALTAAIEDARRANQAKSRFLANMSHEFRTPLNGLSGTSELLAST
                     RLDNEQRGYLETIQAATRSLLSLVEDVLDISAIEAGKLKLKLEAFDLRELVEQIDLLL
                     RPDARARHLDYVVAVAPDVPVALRGDEGHLRQVLVNLLSNALKFTPSGEVRLAIEAAS
                     PAVDGRQRLRFTVSDTGIGIPASARAKLFEAFEQADNSLSRRHAGTGLGTTIAKGLTE
                     AMGGAIGFESSENIGSRFWVELPFELQGVDAAPAVPVREPATLVPAPVQGEGGNVIAF
                     ADPFLRHRARVKPMRVLIADDHAANRMVLQGVLQKAGHRVMAVEDGEEALDALAVGDF
                     ELAVVDLHMPSLSGIDMLRQLRVMEAGGQARTPVVVLSADATPEAVQACKDAGARAFI
                     AKPFAVAKLLDGIAEIALGGSIGTPVDIPRDNVAANNDDILDASVLDEFLAIGMGPAF
                     EANFIGQCVADARMILTRMRRSGEAADWEQVHEQAHAMKGVAGNLGLLQLAALAGQVM
                     RMTGFELARDWRRHCDTLGERLRRGELALAARGNWHPAREDSQ"
     gene            complement(24684..25571)
                     /locus_tag="PK399_00385"
     CDS             complement(24684..25571)
                     /locus_tag="PK399_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPW11617.1"
                     /translation="MAILEKLTPVSRYSALRVVESADGTHWCHMHADLVDNPGRACFK
                     PNLVNEIIQYQWTLGDRMRRTRGNEAGTLQHVVLASDCDAFNLGGDLEYFCDSIRRQD
                     RHALLTYARQCVRGVHAFHTGLDAGAHSIALVQGDALGGGFEAALSCHTIVAEEGVGM
                     GLPEVLFDLFPGMGAYSFLCKRIAPHQAEKLMLSGQIHSSEELFKMGLVDVLVPRGQG
                     VQAVEDVIRANRRIPHARAAMHRVRAMAQPVTLEEMMAITEVWVDTALQLGEKSLRTM
                     ERLVRAQYKRHGHLDAATA"
     gene            complement(25736..27412)
                     /locus_tag="PK399_00390"
     CDS             complement(25736..27412)
                     /locus_tag="PK399_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW11618.1"
                     /translation="MSSERPWLAHYPAGVPAEIDLDEFASINDIFDSAVQKYRDKTAF
                     ISMGKSITYGELERLSRDFAGYLLGELKLKKGDRVAIMMPNCLQYPVAILGVLRAGLT
                     VVNTNPMYTARELKHQLVDSGASVLVVMDNFGNVAQEVLVQVPGVRAVTTGLGDMLGF
                     PKGLIVNFVLKHVKKMVPDFDIPGAVRFRDTLTLGRMHQLPVIRNTPEDLAFLQYTGG
                     TTGVSKGAMLTHRNLVANMQQAASWVGTNVKLGEELIVTALPLYHIFALTSNCLVFMK
                     FGAANLLITNPRDMPGFVKEIKSVRFTAITGVNTLFNGLLNTPGFDQVDFSRLHLTLG
                     GGMAVQRAVADKWKQVTGVTLAEAYGLTETSPAVCINPLDLRAYNGSIGLPVPSTHVC
                     IKDDDGNVLPMGEVGELCVKGPQVMKGYWQRQEETDKVMDANGWLHTGDMARMDEQGY
                     FYIVDRKKDMILVSGFNVYPNEVEDVIAMMPAVLEVAAIGVPDDKSGEAVKVFVVKKD
                     PALTADAVKAFCRENLTGYKQPRFVEFRDELPKSNVGKILRKELRSPSAS"
     gene            27530..27982
                     /locus_tag="PK399_00395"
     CDS             27530..27982
                     /locus_tag="PK399_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW11619.1"
                     /translation="MQQFRGFQRIATALAMTACLWLGACAKDDPEQAVRQQVEAMQAA
                     IDARDAGDVEDLIAEDFVGNEGMDRRAIRQLAAAVFLRHKTVSAKLGPVSVELRGSTD
                     AIARFSVLATGSSGGFLPESGQVYQVESGWRLVDGEWKLLNASWTPNL"
     gene            28013..28900
                     /locus_tag="PK399_00400"
     CDS             28013..28900
                     /locus_tag="PK399_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW11620.1"
                     /translation="MSPSGDINPSHLQLPPGPWATLLDGLCARFPRITRETWLQRFAR
                     GRVQDADGQALAADARYRAGTVVRYFREVDDEPRIPFEAKILFADADLVVADKPHFLP
                     VTPAGRFVDETLLARLRQQLDNPALVPLHRIDRETAGVVMFSANPASRAAYHALFQQR
                     AVEKAYEAIAPPLPALAMPHVRRSQLRAGEPFFRMREGDGPVNSETRIEVMQRGLHAW
                     RYGLHPVTGRKHQLRVHMAALGAPIANDSLYPAMSMRTPDDFGRPLQLLAKALRFIDP
                     LDGTARMFESRQALVLVDA"
     gene            complement(28945..31704)
                     /gene="acnA"
                     /locus_tag="PK399_00405"
     CDS             complement(28945..31704)
                     /gene="acnA"
                     /locus_tag="PK399_00405"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HPW11621.1"
                     /translation="MADSFATRARLDVNGTSYAYASLAKLGEQFDIARLPYSLKILLE
                     NLLRHEDGGVTVGRDHIEALARWDAQAEPATEIAFMPARVVLQDFTGVPCVVDLAAMR
                     DAVVKLGGKASQINPLIPSELVIDHSVQVDVFGRADALDLNGKIEFQRNAERYSFLRW
                     GQKAFGNFKVVPPNTGIVHQVNLEHLARVVVERDIDGDTWAFPDTVFGTDSHTTMING
                     LGVLGWGVGGIEAEAAMLGQPSSMLIPQVVGFQLTGTLPEGATATDLVLTVTQMLRKH
                     GVVGKFVEFFGNGLQHLPLADRATIANMAPEYGATCGIFPIDAESITYLRLSGRSEAQ
                     IALVEAYAKAQGLWHDAMSPHANYSATLALDMASVRPSLAGPKRPQDRVLLEDVQANF
                     ADNLGPLTANRKARNPVVQDFKDEGGAQPQAEHLAVRPRSMIRIRDVDAELTDGSVVI
                     AAITSCTNTSNPAVMLGAGLLARNAVAKGLKAAPWVKTSLGPGSLVVTDYLRKAGVLD
                     DLEQLGFFVVGYGCTTCIGNSGPLPDEVSKGIAENELVVASVLSGNRNFEGRVHPEVK
                     ANYLASPPLVVAYAIAGTVDIDLTRDPLGKDGDGNDVYLRDIWPSNKEIGDSIAATVG
                     PELFAQNYADVFKGDSRWNGIASPEGESFQWDAASTYIKNPPYFDGMTMHVGHIEDLH
                     GMRVLGLFGDSITTDHISPAGNIKGSSPAGRFLQERGVQPADFNSYGSRRGNDDVMVR
                     GTFANIRIKNLMFGGEEGGNTLYFSSDAKPEKLAIYDAAMKYKADGVPLVVIAGKEYG
                     TGSSRDWAAKGTNLLGVKAVIAESFERIHRANLVGMGVLPLQFIDGDNAMELGLDGSE
                     TFSITGLADGASKTATVTATKAGGSTIAFQARVLLATPKEVDYFRHGGLLQYVLRQLA
                     GKQAA"
     gene            31862..32059
                     /locus_tag="PK399_00410"
     CDS             31862..32059
                     /locus_tag="PK399_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPW11622.1"
                     /translation="MEATVAERGQITLPKPVRDALGLAKGSVLKVELEGGRIVLSKHV
                     DDAISRVRGKFALDLPAGGKG"
     gene            32064..32462
                     /locus_tag="PK399_00415"
     CDS             32064..32462
                     /locus_tag="PK399_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPW11623.1"
                     /translation="MIAVDAPVLVELLTDGPRADAAETSLRQALGSGRVVVCDAALAG
                     VCASLRNGADALSALEDMGVHYSAVESKSALRAGEMQRRQRQRNDETRPLADFLVGAH
                     ALLQCDALITYETTFHRDYFKGLKLIVPKA"
     gene            32502..35093
                     /gene="acnB"
                     /locus_tag="PK399_00420"
     CDS             32502..35093
                     /gene="acnB"
                     /locus_tag="PK399_00420"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HPW11624.1"
                     /translation="MLEAYRHHAAERAALGIPPLPLSAQQTAEVIDLLKDPPAGEAQF
                     LVDLITHRVPAGVDDAAKVKASYLAALAFGSEANPLISRARATELLGTMLGGYNVHPL
                     IELLDDAEVGAIAAEALKHTLLVFDAFHDVQEKANAGNANAQAVLQSWANAEWFTSKP
                     EVPASLTITVFKVPGETNTDDLSPAPDATTRPDIPLHALAMLKNSRPDAPFAPEEDGK
                     RGPIAQILALKEKGHLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPFIPNKRFGGVCL
                     GSKIAPIFYNTMEDAGALPIELDVSQMHHGDVVELRPYDGKALKDGAVIAEFQVKSEV
                     LFDEVRAGGRIPLIIGRGLTAKAREALGLPASTLFRLPQQPADTGKGFTLAQKMVGRA
                     CGLPEGQGVRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPVDVKTHHTLPDFISTRGGISLRPGDGIIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGTLQPGVTLRDLVHAIPLYAIKSGLL
                     TVAKQGKKNIFSGRILEIEGLPDLKIEQAFELADASAERSAAGCTVRLNEAPIVEYLT
                     SNITLLKWMIAEGYADARSLARRIAKMEAWLASPQLLQPDADAEYAAVIEIDLDDVVE
                     PIVCCPNDPDDAKTLSDVAGATIDEVFIGSCMTNIGHFRAAAKLLEGKRDIPTRLWVA
                     PPTKMDAAELTKEGHYGTFGAAGARMEMPGCSLCMGNQAQIREGATAMSTSTRNFPNR
                     LGRNTNVYLGSAELAAICSRLGRIPTREEYMADIGVVNANGEKIYRYMNFDKIGEYKD
                     VADTVAA"
     gene            complement(35476..36084)
                     /gene="hflD"
                     /locus_tag="PK399_00425"
     CDS             complement(35476..36084)
                     /gene="hflD"
                     /locus_tag="PK399_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007964280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HPW11625.1"
                     /translation="MSDMEDRVLALAGVLQALAQVRRIAETGQSETAQAEVALDSVFR
                     IDAATTKAVYGDAGAVNAGLRLLHNYLANNERDETLAKLGLSVLQLERRFQGDAGIVH
                     KVQTGIIALSAPAERLGSTHPDVIAGLGSLYADTVSTLRPRVMVQGNPHYLGQAGVVA
                     EVRALLLAALRSAVLWRQNGGSLWDFAFKRRDMAAAIDALLR"
     gene            complement(36081..37205)
                     /gene="mnmA"
                     /locus_tag="PK399_00430"
     CDS             complement(36081..37205)
                     /gene="mnmA"
                     /locus_tag="PK399_00430"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009601444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPW11626.1"
                     /translation="MNAGRIVVGMSGGVDSSVAALLLRDAGFDVTGLFMQNWADDGSG
                     DCRAEDDRRDAVAVSGKLGLPIRFRDFSREYWDGVFQHFLAEYAAGRTPNPDVLCNRE
                     IKFKHFLDAAHDLGADAIATGHYARVDCVAGEYRLLRAKDRGKDQSYFLHQLGQRQLA
                     ATRFPLGELHKDEVRRIATEQQLPTAAKKDSTGICFIGERDFREFLGQYMPARPGEIR
                     DPAGHRVGEHPGVFYYTLGQREGLHIGGVRGRPQAPWFVVGKDVERNLLYVDQGHDSP
                     WLQSQSLRTETMHWIAGAAPASRFNCTAQTRYRQPDEPCDVQVLADGSASVRFARPQR
                     AVTPGQSLVLYDGDVCLGGAVILHTDAPAVPHRLTRNDAA"
     gene            complement(37202..37663)
                     /locus_tag="PK399_00435"
     CDS             complement(37202..37663)
                     /locus_tag="PK399_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007964277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPW11627.1"
                     /translation="MSYREGRFWQPDVTVATLVVRDGRLLCVEERANGQLVINQPAGH
                     LEPDESLIEAAVRETREETGWNVRVTDLVGAYQWKAETGRHYLRFAFAAEPIHQVPDA
                     RLDEGIVQALWLAPSELQALASRHRSPLVWRCVADYLGGSRHPLDLVRQLA"
     gene            complement(37665..38135)
                     /locus_tag="PK399_00440"
     CDS             complement(37665..38135)
                     /locus_tag="PK399_00440"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPW11628.1"
                     /translation="MLTTRLALTALLVLAAPGARAADPVLETLLKAADIPFKVDEDGD
                     YKIVYEWSKDKRSQLVYVSGTTEELAGVTLYEIFSPASVLADGAIDPALARRLLGENA
                     TYKFGAWEISGKNLYFGGKVPAGISASHFEALVNTVASTADDMEKELTPGKDDL"
     gene            38224..38553
                     /gene="clpS"
                     /locus_tag="PK399_00445"
     CDS             38224..38553
                     /gene="clpS"
                     /locus_tag="PK399_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HPW11629.1"
                     /translation="MPQTPDNPHHEHDHGVVVAPAKPDTARPPLYSVFLLNDDYTPMD
                     FVVDVLMRFFTLDLERATQVMLHVHTRGRGVCGVFTREIAESKVAQVNEYARLNQHPL
                     LCTMERA"
     gene            38627..40906
                     /gene="clpA"
                     /locus_tag="PK399_00450"
     CDS             38627..40906
                     /gene="clpA"
                     /locus_tag="PK399_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HPW11630.1"
                     /translation="MFSKDLEHSIGQCYKRARDARHEFMTVEHLVLALLDNVSAEAVL
                     KAVNADIPRLRHELEEAIEVSVNRLAEDDGRDTQPTLGFQRVLQRAVYHVQSSGKKEV
                     TGANVLVAVFGEKDSHAVYFLNQQDVHRLDVVNYISHGVARQGDEQAPQSQDGEGKAE
                     GGEGESKSDPLAEFTSNLNDLALAGKIDPLVGRSEEVERTIQVLCRRRKNNPLYVGEA
                     GVGKTALAEGLAKRIVEGDVPEVLVEAVIYSLDLGALVAGTKYRGDFEKRLKGVLAAL
                     KKLPHAILFIDEIHTIIGAGSASGGTMDASNLIKPVLANGELRCIGSTTFQEYRGIFE
                     KDRALARRFQKIDIVEPTVGEAIEILKGLKPKYEAHHGVSYADDAIKAAVDLSVKHIG
                     DRLLPDKAIDVIDEAGARQRLMPMDARKSLIDVEEIETIVAKMARIPAKQVSSSDKDV
                     LQHLDRNLKMVIFGQDPAITSLTSAIKLARSGLGNPDKPIGNFLFAGPTGVGKTEVTK
                     QLAMQLGIELVRFDMSEYMESHSVSRLIGAPPGYVGFDQGGLLTEKIVKTPHCVLLLD
                     EIEKAHPDIFNILLQVMDRGVLTDTNGREANFRNVILVMTTNAGATQAARRSIGFTKQ
                     DHSTDAMEVIRRGFTPEFRNRLDAIVQFQALGFDHILRVVDKFLIELEMLLHEKQVSL
                     SATPAARDWLAQHGFDPMMGARPMARLIQDRIKRPLADELLFGKLVAGGRVSLDAVDG
                     ELALDVQAEPEKLLPAVVE"
     gene            complement(40961..41179)
                     /gene="infA"
                     /locus_tag="PK399_00455"
     CDS             complement(40961..41179)
                     /gene="infA"
                     /locus_tag="PK399_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPW11631.1"
                     /translation="MAKDDVIEFEGTISETLPNTMFRVKLENGHEIIAHISGRMRKNY
                     IRILTGDRVKVEMTPYDLTKGRITYRHR"
     gene            complement(41292..42041)
                     /gene="aat"
                     /locus_tag="PK399_00460"
     CDS             complement(41292..42041)
                     /gene="aat"
                     /locus_tag="PK399_00460"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPW11632.1"
                     /translation="MNLRIVQLDDDPRASFPPVMQALADPDGLLAFGGDLSPTRLLNA
                     YRQGIFPWFSEDEPPLWWSPSRRAVFRTDGVHLSSRRRRELRKSALVVRADTCFRRVI
                     EACAHLPRPGQDGGTWITGAMVEAYVELHRLGHAHSIEVFDGGALVGGLYGLAIGRMF
                     CGESMYSAQTGASTVALATLAMRLHAWGWPLIDAQVPNGHTRRLGVETWPRTAYLQAL
                     EALRDSDGLPGTWTRDFGEIHASSLARPAQV"
     gene            complement(42038..43171)
                     /locus_tag="PK399_00465"
     CDS             complement(42038..43171)
                     /locus_tag="PK399_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW11633.1"
                     /translation="MMPSARIHRALSEILAADWDALHDGRNPFVAHAFISGLETTGCL
                     REDWGWTPCHLGLWQGDRLVAAAPGYLKSNSHGEFVFDHAWAHAYARHGLDYFPKWLG
                     AVPYSPVTGPRLLARDEASKRALLDAIGGFLEQSGLSSAHVNFHDATDEKAFDAAWLL
                     REDIQYHWHNAAGWADFESFLAAMDHKHRKNIRQERRKVREAGITFRIVHGGEASDGD
                     FAAMHGFYLQTFAEYGNSPALTLDFIRHLARELPRQLVIVLAEREEKPIAGALCLRGA
                     DTLYGRYWGAGELHPGLHFEACYYQGIEYCLREGLARFEPGAQGQHKIARGFLPSIVR
                     SRHRIADPRFAEAIGAWCAEERMATREHLRQLQAHSPFRAPAQ"
     gene            complement(43168..44121)
                     /gene="trxB"
                     /locus_tag="PK399_00470"
     CDS             complement(43168..44121)
                     /gene="trxB"
                     /locus_tag="PK399_00470"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPW11634.1"
                     /translation="MTPTKHTRLLILGSGPAGWTAAVYAARANLKPVVITGMAMGGQL
                     MTTTEVDNWPGDAHGLMGPDLMARMQAHAERFDTETVFDHIHTADLSKRPFRLVGDSG
                     EYTCDALIIATGATAKYLGIPSEEAFKGRGVSACATCDGFFYKDQDVAVIGGGNTAVE
                     EALYLSNIARKVYLVHRRDTLRAEKIMQDKLFAKIEAGKIEPVWHHTVDEVLGNDAGV
                     TGMRVKSMLDGSTREIAVHGFFVAIGHSPNTSLFDGQLAMKNGYLSIQTGLEGNATQT
                     SVPGVFAAGDVADQVYRQAITSAGFGCMAALDAEKFLDEAH"
     gene            44394..46769
                     /locus_tag="PK399_00475"
     CDS             44394..46769
                     /locus_tag="PK399_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPW11635.1"
                     /translation="MARTASETGKSSRKPATKSATPRAAPNPRRQRLWRDLALIAVAP
                     LLLFLLASLVTYSPADPGWSHSGSITAPLHNACGRVGAWIADVLLYLTGYVAFLLPIV
                     LGAIAWIALFGMDSDGDGDADLGPALRLVGIVGFLVASCGLLYLRISGSNDFSAGSGG
                     ILGQLTGRSLLRGFGPVGANLFLVSLFLISVTLATGLSWLAVMDRIGAFALSLPGLLT
                     RGARKGAQQATDWSEARAFREERVEARKVDSELRAKREKVRIEPPPAPVVEKSERAKR
                     EQQIPLFHVADGSGIPPLALLDDPKPQPKGYSEETLETLSRQIEFKLKDFRIDAEVVG
                     AYPGPVITRFEVQPAPGVKVSQISSLDKDIARGLSVKSVRVVDVIPGKSVIGLETPNT
                     HREMIYLSELLRSKEYDKSASPLTLALGKDIAGRPTVADLARMPHLLVAGTTGSGKSV
                     AVNAMVLSLLYKASAKELRMLMIDPKMLELSVYQGIPHLLAPVVTDMKEAGNGLRWCV
                     AEMERRYKLMSAVGVRNLAGFNKKVKDALDSGQPMMDPLFKPNPDLDEVPRQLETLPF
                     IVIFIDEFADMMMIVGKKVEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIP
                     TRIAFQVSSKIDSRTILDQSGAETLLGHGDMLYLPPGTAMPDRVHGAFVSDEEVHRVV
                     EHLKQSGGPDYIEGVLEESQTMYDGSSVGATGLPEASSGGGDESDPLYDEAVKIVTET
                     RRASISGVQRRLKIGYNRAARLVEAMEAAGVVSTPEHNGDRTVIAPPPPRP"
     gene            46845..47477
                     /gene="lolA"
                     /locus_tag="PK399_00480"
     CDS             46845..47477
                     /gene="lolA"
                     /locus_tag="PK399_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HPW11636.1"
                     /translation="MSTFGMKPATTILSAALLLAANIANASARDELNAFTKGLKGLDG
                     QFTQQVFDARGKQKETSSGRVAVSAPRLFRWEYVRPYPQLIVADGKTVWVHDPDLQQV
                     SRRAQGVEEANSPLAILLDPSKLDRDFVVKDAGKTGGIEWLELTPKQAEAPFKSARLG
                     FANKGLAQMEYVDALGQRTRISFTGWKRNPAFARGTFVYVPQKGVDVIGG"
     gene            47584..48276
                     /locus_tag="PK399_00485"
     CDS             47584..48276
                     /locus_tag="PK399_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11637.1"
                     /translation="MDRKGLNRTAIAVALALVTAGAVVAQAGTPATPNAVVVEGHSND
                     LVAAGNALDSRILASIDDATAAALIGALETRFAGREVALRMGEVESTRASLRDIALHG
                     TAQIRLGQDAGWMPIRFEALYDTDTQAVLSPAIVLEGSAVAAAPKLPLPGLQAQVRAA
                     MGDEFASQRVDVSLRQAQVIGNDGRRLVVRAQGIARFDGSDTAPVTVRALYEQGSGRW
                     LDAQYDFDVVGG"
     gene            48365..49735
                     /locus_tag="PK399_00490"
     CDS             48365..49735
                     /locus_tag="PK399_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPW11638.1"
                     /translation="MSLLPEESGSASSPDVSSPDVVAMRPLAERMRPRSLDDMVGQRR
                     LLAPASALRRAVEAGRVHSMILWGPPGCGKTTLALLLARYADAEFVAISAVLSGLPQV
                     REVLAEAQARFEGGRRTVLFVDEVHRFNKAQQDAFLPHIERGSILFVGATTENPSFEL
                     NSALLSRCRVHVLEAVSTDDIVVALRHALDDAERGLGGRGLAVAENALREIAGAADGD
                     VRRALTLLEIAADIADGEGGTISGATLHQVLADRTRRFDKGGDQFYDQISALHKCVRS
                     SNPDAALYWLARMLDGGADPAYMARRLTRMAVEDIGLADPRALQMAIEAWDAYDRLGS
                     PEGELALAQLTIYLASTAKSNAGYRAWNAARGDVDALGTQDVPLHFRNAPTKLMKSLG
                     YGKDYQYDHDADGGIALDQTGFPDAMGERVYYAPTPRGMEGKLKEKLDALRAARQQAR
                     DAKGSR"
     gene            49732..50133
                     /gene="crcB"
                     /locus_tag="PK399_00495"
     CDS             49732..50133
                     /gene="crcB"
                     /locus_tag="PK399_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HPW11639.1"
                     /translation="MNAWWQQLVLVMLGGALGAAGRFWLGGALLRQFGSGIPWGTLAA
                     NLIGSFAVGYIAVWLEGRGPAALYWRAFLIVGLLGGLTTFSALMLESLLLARTHRNDL
                     LLAYLGGTLVAGLLLVWAGARLAEALRAASP"
     gene            complement(50149..51465)
                     /locus_tag="PK399_00500"
     CDS             complement(50149..51465)
                     /locus_tag="PK399_00500"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPW11640.1"
                     /translation="MQGRSLRNGNQALEAGTSDQQRQPCHVARPEGVALRRHQSAAGD
                     AVALPRRVEHVAPQSDPEQPESRVWNPCLAGLPMTHRAKADTEELRNVLSALAGELAC
                     LAELFWGNAPDAWALAVLTGSGQPGPGRHYAKCVWRSRDVEGPYVAWTGNPSLTQRDL
                     DPNRPSPITSTGHAQFIELKDGSWWTVFLGTRPYRGNQYNLGRETFLLPVSWTDDGWP
                     MVLPRGARVPAVAQRPALPRDPSPPPMSGPITWSETFADSPGPQWLTLHAPQLPWFRT
                     GDGGLRIVPGTTSLGAHGAAGNGQPTYLAHRLQHHKAWIEATLEPAGLEVGELAGLAL
                     FQNETHHVVAAVERSDAGAALVLRLRNGKDDPLPGRELKRVPLASNDAALSLRLQLDD
                     QHLDVSWRSGTEHWNTLLNRLDASLLSVDHAGGFIGNTFGPYAVHQ"
BASE COUNT         8785 a        16934 c        17623 g         8298 t
ORIGIN      
        1 ttccagaagg agcagggcat cgacctgcgc aaggatccgc tggccctgca gcgcctgaag
       61 gacgccgccg agcgcgccaa gatcgagctg tccagctcgc agcagaccga cgtcaacctg
      121 ccgtacgtca ccgccgacgc gaccggtccg aagcacctca acatcaagct gacccgctcc
      181 aagctggaag cgctggtcga cgacctggtg aagaagacca tcgagccctg ccgcatcgcg
      241 ttgaacgacg ccggcctgcg cgccagcgat attggtgaag tgatcctggt cggtggccag
      301 acccgcatgc cgcgcgtgca gcaggcggtg accgagttct tcggcaagga gccgaagaag
      361 gacgtcaacc cggacgaagc ggtggccatt ggcgccgcga tccagggcgg cgtgctgcag
      421 ggcgacgtca aggacgtgct gctgctggac gtgaccccgc tgtcgctggg tatcgaaacc
      481 ctgggcggcg tgttcaccaa gatcatcgag aagaacacca ccatcccgac caaggcatcg
      541 cagaccttca gcaccgccga ggacaaccag tccgcggtga ccgtgcacgt gctgcagggc
      601 gagcgcgaac aggcccgttt caacaagtcg ctggccaagt tcgacctgac cggcatcgac
      661 gccgcgccgc gcggcatgcc gcaggtcgag gtgagcttcg acatcgacgc caacggcatc
      721 ctccacgtca acgccaagga caagaagacc ggcaaggagc agaaggtcga gatcaaggcc
      781 ggatccggcc tgagcgacga cgagatccag cggatggtcc aggacgccga ggccaaccgc
      841 gaggaagaca agcgtttcca cgagctggtc accgcgcgca accaggccga tgggctgatc
      901 cacgcgaccc gcaccgcgat caaggacaac ggcgacaagg tgtcgggcga cctgattggc
      961 cgcgccgagg gtgcgatcgc cgagctcgag accgcgatga agggtgacga caagggccag
     1021 atcgatgcca aggccaaggc gctggaagag gccgggcagg cgttgtttgc cgcggcggct
     1081 gcggccgcgc ggccgggtgc cggtggcggc gatgccgggc aggccccggg tgccggtgac
     1141 gacgtggtcg acgccgagtt caccgaggtc aaggacgaca acaagggcgc ctgnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacgacgt ggtcgacgcc gagttcaccg
     1321 aggtcaagga cgacaacaag ggcgcctgat cgcgcaacgc gattgacagc aaagcagcgg
     1381 gcaggccagt ggtctgcccg ttgccgtgaa ggatgcagga aaggtcgatg agcaaacgcg
     1441 attactacga cgtgctgggc gtggcccgca acgcctccga tgacgaattg aagaaggcct
     1501 atcgccgtag cgcgatgaag caccatccgg accgcaaccc cggcgatgca agcgccgagg
     1561 ccgcgttcaa ggagtgcaag gaagcctatg aagtgctgag cgacggtggc aagcgccgca
     1621 tgtacgacca gcacggccat gcggcgttcg agcatggcat gggcggtggc aacgccgggc
     1681 ccggcttcgg tggcgccgac atgggcgaca tcttcggcga catcttcggc aacatcttcg
     1741 gcggcggtgc ccgccagcag ggcccgcgtc gcggggccga catcggctac gtgatggagc
     1801 tcgacctcga ggaggcggtg gcgggcgtcg agaagcgcat cgagatcccg accctggcca
     1861 actgcgaacc ttgcaagggc agcggctctg ccgacggcaa ggtcgagacc tgcgccacct
     1921 gccatggacg tgggcaggtg cgcatgcaac gcggtccatt cgcgatgcag gcaccgtgcc
     1981 cgcactgcgc cggcaccggc aagaccattc ccaacccgtg cccccagtgc gatggaagtg
     2041 ggcgcatcga ggaagagaaa gcgctgtcgg tgaagatccc ggccggcgtg gacaacggcg
     2101 accgcatccg cctggcgggc gagggcgagg ccggcccggc cggcgcgcct ccgggcgatc
     2161 tgtacgtgga agtgcgggtg cgcccgcacg ccatcttcgc ccgcgatggc gacgacctgc
     2221 actgcgaagt gccgatccgg atttcgcagg ccgcgcttgg cgacagcgtg cgtgtcccca
     2281 cgttgggggg cgaggtcgaa ttgcgcatcc ccgtcgaaac ccagacaggc aaggtattcc
     2341 gcctgcgcga caggggcgtc aaatcggtgc gtagccggca ccccggcgac ctgtactgca
     2401 aggtcgtggt cgagactccg gtcaacctca ccaacgagca gcgcgagttg ctggagcagt
     2461 tcgaggcgac gtttaccggc gagaacgcgc gccggcattc gccgaaatcc agcaccttcc
     2521 tcgatggcgt gaagggcttc tgggaccgca tggtgtcctg aatcatgccc atcgtcggaa
     2581 aaaatgaggc gcaattcgca aaaccgggtg gccacggcga cacgcggcgc aaacccgcgt
     2641 agcctgccca atggctcaat cccccgacca gggccccatg tccttccgtt cccccacgga
     2701 aattcccccg cagactccgc cagaccgggg tcggctgctc gacagcctgc agaagcagat
     2761 gctggagcgg gtgttgcctg cgctcgaccg cgcgctgggc aaggtcgacg attatctgtt
     2821 cgaccgcagc cagcagggcg acgaggaatt cggcctgatg gcgttgcgcg agttgcgtcg
     2881 ccagcgcggc cagatcgcgc gccgctatga ggacgtggta caggccgggt tccgcaacct
     2941 ccgcagcgcg agcgaatggt ttcccgcgga tggtcgttca tccgacctga gcctgttgtc
     3001 ggagcaggag ctggaggagc aactggcggt cgagcaactg gcggatgcac tggttcgcgt
     3061 gcatggcccc ggggtcgaga tgtccggcaa gcgcattgcc cacattgccg gccgcgagga
     3121 tctgcaggcg caggacaatc cgcttgcgcc tgccttcctg gccgctgcgc tgcgtaccgc
     3181 cctggccccg tccgagctgg aagccggcgt gcgcatcgtg gtgttcaagt tcttcgaacg
     3241 cgaactcggg gcggtgctgg gtgccgccta cgaacgcgca aacacgctga tgatcgccgc
     3301 cggcgtgttg cccgagctgc gtgcgacggt caaggccaca gcgcaaccgc aggcgccggc
     3361 gagttccccg gatccgcatg ccccgcaagc accgtcaggc caggtgcagc tgcccgccca
     3421 ggtgagcgcc gtcgaccagg cgatgttcgg caatctcctc ggcctgctgc agggttggcg
     3481 gaccgccatg ggcggcaacc gggcagatgc cgaaggcggc cccctgcagc cgggcgcgca
     3541 acaagcgccg gtcaccaagc tcggttccag tgagttgatg tcgatcctgt ccttgatgca
     3601 gcaggacatg ccgaatgccc cgttgccgcc acgcgaggcc ggcggcgacc atcgcaccct
     3661 tgctgaacag ctgcgcaagg aagtcgcgca gaacgcgcgc aagctcgggg tggcgggcga
     3721 caacctgcac ctggacgggc tggacgagga cgcggtcgac ctggtggcgt tgctgttcga
     3781 cgtactgctc gacggcccgc agttcgacgc caacatccgt cgcaagatcg gccgcatgct
     3841 ggtgccgtac gtcaaggtgg cggtcaagga tcggcgcatg ttcctgttca aggaacatcc
     3901 cgcgcggcgc ctgctcaaca cggtggcaga ggcttgcgag ggcaaccacg gcgaagcgcc
     3961 gcaggagcag gcgctgctca accgcgtcga ctacaccatc gatcgcctgg tcgccgagtt
     4021 caacgaggac gtggcgatct tcgagacgct ggagcaggaa ttgcgctcct acatggcgca
     4081 gcaccgcaag cggttcgagc tggccgagaa gcgcgccacc gaggcgcagc gcggacgcga
     4141 gcgcctggac ctggctcgca gcgcggtcgc ggcggaattg atgcagcacc gcggcgagcg
     4201 tgaattgccg gcggtgatgg acgaattcat cggccgccac gtctcccacc acctgaccca
     4261 ggtgatgctg cgcgatggcc acggctcgcc gcgccatgag caggcgatcc aggccgtcga
     4321 ccgcctcctg ctggcgttcg actacgccga ggtacgcagt ccgctggagg aattgccgac
     4381 gctgccacgt gcgcagttcg aagcgatcct gtccagttcg ggctgcaccg gcgattccgc
     4441 gcaactcgcg atcgactcct tgcaggatgc gctggtgcgg ctgtccgcgg gcgaacgcgc
     4501 agccgacatt cccgggcaca tgcccgagca ggtcatcctg ccggagccga aggccgtcga
     4561 gcccgagccg cagctcgagg tcgtggccgg caacgaccac ctggactacg acgcggccat
     4621 gctcgaccgc atgcgcaagc tccaggtcgg cagctggctg cagctggcga cgtccgcgga
     4681 tcgggtcgag ccgtcgaagg tgtcgtggat cagcccgatt tcctcacgcc tgctgctggt
     4741 caaccgtcgc ggcatccgcg tgctggtggc ctcggccgag gaactggcgg ccatggccaa
     4801 actcggcaag gtcagcctgc gcgagggcga cagcgtgttc gacaatgcca tgcaccaggt
     4861 cgccgatcgg ctcaagaacg ccgccagcca gttgtaggca cgcggccgcg cggcttgtcg
     4921 cggcccgccc tgccggctag cctgcgggca tgacgaatcc cgttcgattg ctggtccatg
     4981 gtgccaacgg gcgcatgggc caaagcctgc aacgcctgtg ccgcgaggaa aacgcagggt
     5041 gcaccgtggt agccgcggtg tcgcggacgg tcgatgcgcg cgtcgtcgat ggcatcccgc
     5101 aattcgcttc cagcgaactg gcgggcgtgc cggccttcga tgtcgccgtg gatttcagcc
     5161 tgcccgcggg cttcgacgcg atcctcggcc tgtgcgtcgc ccgtggcgct ggattggtat
     5221 ccggcacgac cggcttgtcc gtgtcgcagg aagccgcatt ggccgacgcg gcgacacgca
     5281 tcccgctcgc ctgggccagc aacttcagcc tgggcgttgc cgtgctgcat gacctggtcg
     5341 agcgtgcggc gcgtgccttg cctggctggg attgcgatat cgtcgaatcc caccacaccc
     5401 gcaagcggga tgcgccttcg ggcactgcgc tgacgctggg cgactcggcc gggcagggcg
     5461 gcgccacgcc gcattacgcc tcgatccgcg ccggcgacat cgtcggcgag cacctggtgc
     5521 agttcaccac ggcgggcgag cgcatcgaac tgatccatcg cgcgggcaac cgcgacatct
     5581 tcgcgcgagg cgccctgcat gtggcagcgc ggctgaaggg ccgcgccgct ggtcgttatc
     5641 gggtcgccga tctgctctga cggacttccc tggcgctccc tcatcggggt gaatcctgat
     5701 tcgcccgggt gaatcgggat gctggcgacc gctggcacat gccggtacaa ttctcgctcg
     5761 cctgaacccc tccgctccga accggttgaa gccgcttcgg cgcttttctg caacctctcg
     5821 cagggcgatg cctcttgaac atacccgcaa tcctcgtgct tgaagacgga accgtcttcg
     5881 agggcatttc cgtgggcgcc gccggcctca ccgtgggcga agtggtgttc aacaccgcga
     5941 tgaccggcta ccaggaaatc ctcaccgacc cgtcgtacgc gcggcaattg gtgaccctga
     6001 cctatccgca catcggcaac accggttgca ccgcgcagga cgacgaagcc gaccgcgtct
     6061 gggccgccgg cctgatcgtg cgcgacgtgc cgcgccgcgc cagcagctgg cgcagcgaga
     6121 tcgcgctgcc ggagtggctg cgcgcccgcg gcatcgtggc gatttccggc atcgacaccc
     6181 gcaagctcac ccgcatcctc cgcgaccgcg gctcgcagaa cggcgcgctg atggcggggg
     6241 ccgctcccga cgcgcaactc gacgtggaca aggccgtcga ggccgcacgc aagttccccg
     6301 gcctgaaagg catggacctg gccaaggtgg tcagtaccca agccaactac acgtggcgcg
     6361 acgggcagtt ggacctcgac cgcaatgcct tcgtgcgcgc cgattcgagg ttcaaggtcg
     6421 tggcctacga cttcggggtc aagcacaaca tcctgcgcat gctggccgaa cgcggttgcg
     6481 acgtgaccgt ggtgccggcg cagacgcctg ccgccgacgt gctggcgatg cagccggacg
     6541 gcgtgttcct ctccaacggc cccggcgatc cggcgccctg cgactacgcg atcgccgcga
     6601 tccgcgactt catcgcggcg aaggtgccga ccttcggcat ctgcctgggc caccagttgc
     6661 tggcgctggc cgcaggcgcg cggaccttga agatgccgca cggccaccac ggcgccaacc
     6721 atccggtgca ggacctgacc ggcgcgggcg gcggcaaggt gatgatcact tcgcagaacc
     6781 acgggttcgc ggtggacgaa accagcctgc cggccaacgt cagggtgacc caccgctcgc
     6841 tgttcgacgg cagcaaccag ggcatcgaac tgaccgacgc cccggccttc agtttccagg
     6901 ggcatcccga agcctcgccc ggcccgcacg acgtggcacc gctgttcgat cgattcgttg
     6961 cgctcatgga gacgcactga tgcccaagcg cacagacctc aagaccatcc tcatcatcgg
     7021 cgccggcccg atcgtcatcg gccaggcctg cgagttcgat tactccggcg cgcaagcatg
     7081 caaggcgctg cgcgacgagg gctatcgcgt ggtgctggtc aattccaatc ccgccacgat
     7141 catgaccgac ccggagatgg cagacgccgt ctacatcgag ccgatcaact ggcggatggt
     7201 cgagaagatc atcgccaagg aacgcccgga tgcgctgctg ccgacgatgg gcggccagac
     7261 cgcgctgaat tgcgcgctcg acctggccga caatggcgtg ctggagaaat acggcgtcga
     7321 gctgatcggc gccaagcggg aggccatcat gatggccgag gatcgcgagc tgttccgcgt
     7381 cgcgatgaag gagatcgggc tggaatgccc gaaagccgaa gtcgcgcgca cgctggagca
     7441 ggcgctggat atccagaccc gcgtcggtta tcccacgatc atccgcccca gtttcaccct
     7501 aggcggcagc ggcggcggca tcgcctacaa ccgcgaagag ctgatcgaga tcgtcaatcg
     7561 cgggctggaa ctctcgccga ccagcgaagt gctggtcgag gaatcggtgc tgggctggaa
     7621 ggaattcgag atggaagtgg tccgcgacac ggcggacaac tgcatcatcg tgtgcagcat
     7681 cgagaacctc gatccgatgg gcgtgcatac cggcgacagc atcaccgtcg caccggcgca
     7741 gaccctgacc gacaaggaat accagcgcct gcgcgatgcc tccatcgcgg tgcttcgcaa
     7801 gatcggcgtg gataccggcg gcagcaacgt gcagttcggc atcaacgcgc agaccggtcg
     7861 cgtcgtcgtc atcgagatga atccgcgggt gtcgcgttcg tcggcgctgg cctcgaaggc
     7921 caccggcttc ccgatcgcaa aggtcgcggc caagctggcc gtcggctaca cgctggacga
     7981 gctgaagaac gagatcaccg gtggcaagac cccggcgtcg ttcgaaccgg cgatcgacta
     8041 cgtcgtcacc aagatcccgc gcttcgcctt cgagaaattc ccggccgcgg atgcgcgcct
     8101 gaccactcag atgaagtcgg tgggcgaagt gatggcgatc ggccgctgct tccaggaatc
     8161 gctgcagaag gccttgcgcg ggctggaaac cggcaaggtc ggcctggatc cgaccggcct
     8221 ggacctggtg gacgaggacg acctggtcaa gttgcgccgt gagctcaagg agccgggccc
     8281 ggagcgcatc ttcttcctcg ccgatgcatt ccgcgccggc atgtcggtgg aagaggtcca
     8341 tgcgttgtcg ttcgtcgacc cgtggttcct cgaccagatc gaggaactga tcgatgccga
     8401 ggtcgacgtg gtccaccgcg gcctggacgg cttggacaag cgccggctgc gcgcgctcaa
     8461 gcgcatgggc ttctccgacg cgcgcatcgc gcagctggcg cgcaccagcg aatccgcggt
     8521 gcgtgcgctg cgcaaggcgt tccacgtgcg cccggtgtac aagcgcgtgg acagctgcgc
     8581 agccgagttc gcgaccacga ccgcctacct gtattcgacc tacgaggacg aatgcgaggc
     8641 gcagcccagc gaccgtcgca aggtcatggt ccttggcggc gggcccaacc gcatcggcca
     8701 gggcatcgag ttcgactact gctgcgtgca cgctgcgctg gccctgcgcg aagacgggtt
     8761 cgagaccatc atggtcaatt gcaacccgga aaccgtctcg accgactacg acacctccga
     8821 ccgcctgtac ttcgagccgc tcacgctgga agacgtgctg gagatcgtcg agctcgagaa
     8881 gccggtcggc gtgatcgtgc agtacggcgg ccagacgccg ctcaagctgg cgaaggcgct
     8941 ggaagccaac ggcgtgccga tcatcggcac ctcgccggat tcgatcgacc tcgccgaaga
     9001 ccgcgagcgc ttccagaagc tggtcgattc cctcggcctg aagcagccgc cgaaccgcac
     9061 cgcacgcagc gccgaggaag ccctggccca tgcgcgcgag atcggctatc cgctggtggt
     9121 gcgcccgagc tacgtgctgg gtggccgcgc gatggaagtg gtgcatgccg acgccgacct
     9181 tgcgcgctac atgcgcgaag cggtgaaggt ctcgcacgat tcgccggtgc tgctggaccg
     9241 cttcctggac aacgcggtgg aagtcgacat cgacgtgatc gccgaccgcg agggcaacgt
     9301 gctggtcggc ggggtgatgg aacacatcga ggaagccggc gtgcattccg gcgactcgtc
     9361 gtgctcgctg ccgccgtatt cgctgtcggc ggacacccag gcgcgcttgc gcgtgcaggt
     9421 cgtggcgctg gccaaggcgc tgaatgtcat cggcctgatg aatacccagt tcgcggtgca
     9481 gaccaatggc gacggcgagg acacgatctt cctgctggaa gtgaacccgc gcgccagccg
     9541 cacggtgccg ttcgtgtcca aggcgatcgg ccagccgctg gcgaagatcg cggcgcgttg
     9601 catggccggc atgaccctcg cggagcaggg ggccacgaag gaaatcatcc ccggctacta
     9661 ctcggtcaag gaagcggtgt tcccgttcgc caagttccag aacgtggatc cgatcctggg
     9721 cccggagatg cgttccaccg gtgaagtgat gggcgtgggc cggagcttcg aggccgcgtt
     9781 cgcgcgcgcc gaggaagccg ccaacatcaa ggcgccgcaa ccgggcaagg ccttcatctc
     9841 ggtgcgcgat cccgacaagg cgcgggtgct gccggtggcg cgctacatgc tcgagcgtgg
     9901 cttcagcctg gtcgccaccc agggcaccgc cgacgtgctg gccgccgccg gcatcgaatg
     9961 cgcgcgggtg aacaaggtga tcgagggtcg gccgcatatc gtcgacctga tcaagaacgg
    10021 cgagatctgc tacatcatca acaccaccga gggccggcag gcgatcgccg actcgttctc
    10081 gatccggcgc gaggccctgc agcagcgcgt cacatactcc accaccgtgt ccggtgcgag
    10141 cgcattgctg cactcgctgg accaccgcgg cgcgggcgac gtgctctcgc tgcaggaact
    10201 gcacaaggaa ctcgcctgat gcacgcaccc atcaccgcga agggcgcact gcgcctgcgc
    10261 gccgagctgg aagaactgaa gtcggtcaag cggccggaag tgatcgccgc gatcgccgaa
    10321 gcgcgcgcgc acggcgacct caaggaaaac gccgaatacc acgccgcccg cgaacagcag
    10381 gggttcatcg aggggcggat caagcacctc gagggcgaac tctcgcatgc gcaggtcatc
    10441 gacatcgcca acctgtcggt gggcgacaag gtcgtgttcg gcgccactgt cgaacttgcc
    10501 gacgtcgaca cggacgagca gaagacctac cagatcgtgg gcgacctcga ggcagacatc
    10561 aagcagggcc tgatcgcgat ttcctcgccg gtcgcgcgcg cgatgatcgg caagcacgag
    10621 ggtgacgcga tcaccatcga agccccgggc ggcatccgcg aatacgagat catctcggtc
    10681 aagtacctgg gctgagcgcg tgccgcggtt gaccctgctg ctgccggcga ccacgcgctt
    10741 cgccggcgtg gccttgccgc aggcgctcgc ggctgcgctc gggcgtgccg atcgcgcgat
    10801 gcatgaggca ggcgaggacg cgcaactcgc gcggcacttc cgcttgctgc cgaatcgttg
    10861 ggcgcacgcg gcattgaccc gcgtggtcga cggcggccag gaggatgcgc gcctgtccgg
    10921 ctggctgcgc gcggatccgg cgtacatccg tccggacatc aatggcgcgc gcctgctcgc
    10981 cacgggtgac ggccttggca tcgacgcggc tgacgtggag gcggtgctgc cggcgctgcg
    11041 cccgctgttt ggtgatgttg gtttcgggct ggatgcaccg catccaggtc gttggtacct
    11101 gcgcttgccg aaggaagccg cattgcctgc gttcgcatcg cccacgcagg ccttgggtga
    11161 tgacgtgttc gagcatattc cggccgcgcc cgaggcgcgc cgctggcggc tgctggccag
    11221 cgaggcgcag gtggtgttgc acaaccatcc acgcaacgcg acgcgtgccg ccgccggcaa
    11281 gccgccgatc aacgcgctgt ggttctgggg cggcggggta ttgcccgacc acgtggcgag
    11341 cgcatccccc acgctgtatt ccgacgatcc cttgctgcat gggctggcga aactcggcac
    11401 gctggacgcc atgccgctgg atgccgcgaa ggagtcggag gacgacgggt tgatcgattt
    11461 gcgggcagtg cgcgatccgc gggcggacgt ggcgcgatgg ctgtcgcccg ccgcgcaggc
    11521 tgcatcgcgt ggcgaggtcg tattcgactt cgccgatggc atggtgttca cggttgcccc
    11581 tggccagcgt tggcggttct ggcgcaagcc cgcagcccgg atcgacgcat gagcgccacc
    11641 cggcgcatcg tccgtcgcga cgccgtcgag gcgcgtggcg actggccggc gtcatggccg
    11701 cctttgctgc gccgcgtgca tgccgcgcgt ggcagcgatg cgcgggaggc gatgccgcgg
    11761 cttgccgacc tgctgccgtt caccgggttg gccggcatcg aacacgcggt ggagttgctg
    11821 cgcgatgcgg tccaccgcga tgcacgcatc ctggtggtcg gcgatttcga ttgcgacggt
    11881 gccaccgcct gcgcggtcgg cgtgcgcggg ctgcgcatgc tcggtgcgaa ccacgtgatg
    11941 catgcggtac cgaaccggat cgtgcacggc tatgggatga cgcccggcct ggtggaggaa
    12001 ctcgcggcgc tgcacccgga cctggtggtg acggtggacc acggcatcgc ctgccatgcc
    12061 ggcatcgatg cggccaaggc gcgcggctgg caggttctgg tgaccgatca tcacttgccc
    12121 ggcccgacgc tgccgtcggc cgatgccatc gtcaatccca accagcccgg cgacaccttt
    12181 gccagcaagg cgatggccgg ggtgggggtg atcttctacc tgttgctggc gctacgggcg
    12241 cgccttggcg cgtcggcgga cctggcctcg ctgctggacc tggtcgcggt cgggacggtg
    12301 gccgacatgg tggcgctgga tgccaacaat cgcgccctgg tcggcgccgg gttgcgccgc
    12361 ctgcgcgcgg ggcagggctg cgctggactc cgagccctga tcgacgtgtc cggccgtcgc
    12421 gaggccacgc tgtccaccgc cgacatcggc ttcgcgatcg ggccacgcat caatgcagcc
    12481 ggccggctgg aggacatggg catcggcatc gaatgcctgc tcagcgatga cgtcggccac
    12541 gcgcgccaac tcgcctcggt cctgcacggc atcaacgagg aacgccgcgc cgtgcaggcc
    12601 gacatgcagg acgtggcgga ggcgcgactg gatggcgtgg gacaggacgc gcccgccgcc
    12661 tgcctgttcg acgaggactg gcatccgggc gtggtcgggc tggtggcgtc caagttgaag
    12721 gaacgcctgc atcggccggc catcgccttc gcgccggtcg aaccgggcgg ggcggcattg
    12781 cggggctctg cgcgctcgat tcccggcttc catgtccgcg acgcactggc gctggtcgat
    12841 gcgcgccatc cgggcctgat cgatcgcttc ggcggccatg cgatggcagc cgggttgagc
    12901 ctgcaggtgg aggcgttccc ggcgttttgc gacgcgttcc tgcaggtcgc gtcggactgg
    12961 ctcaccgatg acctgctcac cgacgtgctg cgcagcgacg gcgccctggc cgatgccgat
    13021 ttccacatcg acaccgcgct ggccctgcgc gatggcggcc attgggggca gggctatgcc
    13081 gagccgctgt tcgacggcga gttcgacgtg ctggcttggc gcatcgtcgg cgaacgccac
    13141 ctcaagctgg aactgggcgc gggcggaaaa cgcctgaacg ccatccactt cggcggctgg
    13201 gacggcgatg cgccaccgtc acggctgcac atcgcctatc ggttggtgcc cgacgactac
    13261 cgcggcaacg gtgctatcca gttgctggtc gtgcaccgtg aaccggcgtc gtccggactt
    13321 cgcgcttgag cccgaacagc ggtcgcagcc aggcgatgcg gcggatcacc gcgtagccga
    13381 gcaggcacag gccgaacgtg gccacgacca gcagtggccc ttccagcacg ggcgggagat
    13441 gcagcggctt caggttgtgc gcgagcagca cgatcactgt ctggtggagg atgtagaacg
    13501 ggaacaccgc gacggtcagg gtgcgcaaca acggactgtc ctgcggattc cagttgcggg
    13561 catatcccag gatggcggcg atcgcggccc attccatcat tccccagcac gtccagatca
    13621 ggctcatcca tgctgtcggg atcgggtcgg catcatcgaa ttgcagccac gccgccagca
    13681 tcgacagata actggctgcc gccagcagca acatgcggtg gcgatgccgg cgcaatggct
    13741 cccacgcggc atcggtcgat gtcagcaggt agccgagggc gaacaccagc ccgtactgca
    13801 ggtggttgta ggcgtcgtcg acaaggtcgt gggtggatgg cgcgtgcgga tacaccagca
    13861 cccgcgcggc gaccagggcc agcatcggcc accacaggat cccgtggcca ctgcatgccc
    13921 ggcgcacgaa cgccgcaccc ttggcccgcc acgatggcgg cagcaggcgg caggtcgccc
    13981 acaacaccgc cgaatagacc cacaggtagg cgacgaacca gaggtggttc caggtgggcg
    14041 aatccacgca gtcgcccggt gccatgcagt agaggtcgcc gcccttgagg tagtcgtcca
    14101 tgaaaccgag gtagccggcg tggtagccgc ccgggacttt ctcgaccagt tccaggtagg
    14161 cctgcggcgg caccaccacg tacatcccga acagcagcgg caacagcaac cgccaggaac
    14221 gctggcgcag gaaaccaacc gggagtttcg cgcgcaggtg cgcggtggcc acgcccgaga
    14281 ccaggaacag caaggcgagc cgccagggcg cgctgaggat catgaagggc tccagcgcgg
    14341 gaaccagttc agggcttttg acgtgccagt cccagctcac gtagtacatg cccacgtggt
    14401 acgggacgag cagggcgaag gcgaggatgc gcacccagtc gagatcgaag cggcgttgca
    14461 tgcggtcggt tccgtggcga aacagcggcc aacatccgtc cgcttgggtg atcccgccag
    14521 cgtggagtga ccgtgcgatg cggcgcaggg acgggtcgcg ggatgcaggg acgagtcgcg
    14581 ggccatgccg caggagccgc ccgtagaatg cgtggatgga tgcagcggtc ccgacaccct
    14641 tccagcgtta ccagccgtgg cgacgcagtg tcgaaatcgg gttctgggtc gtctcctacc
    14701 tgttgagtgc gatcggcaac agcgtgacca ccaacatgga cgtgagccgg ctggcgcttg
    14761 gcttcgccag ctggcagccg gtggcctggg agagcagcag cgcgatcgcc gcactggcct
    14821 tggtgccggc cgtggcctgg ttcaccaggc gctgcccgct ccacctcgac acctggcggc
    14881 ggatgctgcc tgcgcacctg cttgccagcg tcgtctggtc cgcgctgcac gtgatcgcca
    14941 tggtcgcgat ccgcaaggcg gtctacgcca cccaggggca acactacgat ttcggcacgt
    15001 ggtggtggga gttcggctac gaatacctca aggacatccg ctcctatgcc ggcgtggtgg
    15061 cgaccatcga ggtctaccgc ttcgtgctgc ggcgctggca gggcgaggcc agtctgctcg
    15121 acgtgccgga cgatggcccg ccggtggaat cggtggaccg tcccgagcgt ttcctggtgc
    15181 gcaagctggg tcgcgaattc ctggtggccg ccaacgacat cgaatggatg caggcctccg
    15241 gcaattacgt gaacctgcgc atgcgtggcc acgattaccc gttgcgcagc acgatcggcg
    15301 gcatcgaggg caagctggat ccggaacgct tcgtccgcgt gcaccggagc tacatcgtca
    15361 acctcgacca ggtcggatcg atcgagccgc tggagaccgg cgatgcgcgg gtccacatga
    15421 aggacggcag caacctgcca tgcagccgga cctatcgcgg tgggttgcgg cagcgggtcg
    15481 gcctcgacgc gtgatcgcgg aaccccgttg gacatggcca gtcgttgcat gatggcgacc
    15541 aacagggagt ccaccgcatg aaacgcctgc tcttgaccct cgccgccggt gtcgccgcca
    15601 tcggcatcac cctcgacagc cccgcgcagc acgtgcggcc gcagccgcaa cccgtgatcc
    15661 cggcatggat cgaggtcccg ggtgcggaga cgccggtgac tctggaacgg gcggaggtcg
    15721 aggtcggcac cgcaggcggc ctcgcccgca ccagcgtagt gctcaccctg cgcaatccga
    15781 acgcccggat cctggaaggc accctgcaat tcccgttgca gccgggccag caggtgacgg
    15841 cgttcgcgct ggatgtcggc ggcgtgatgc gcgatgcggt accggtgccc aagcagcagg
    15901 gcaggcaggt gttcgagagc atcgagcgcc gcaatgtcga ccctgcgctg ctggagcaga
    15961 ccgccggcaa tcatttccgc ctgcgcgtgt atccgctgcc ggcgcgtggc acgcgacagg
    16021 tgcgactggt gctggacgaa ccgatgcgcc gcgatggcga cgagtggcgg ctggccttgc
    16081 ccgctggact gctgtcggcg gcctctgcgg tgtccttgcg cgtgcaggac gcggagaaag
    16141 cgccgatcct cgacggcacc ttctcgcgcc cgcgatggcg tcgcgatggc gatggccagc
    16201 gcaccgaatt gcagcgtggc gatgctggct cggtagccgg cctttccctg cgtttcccga
    16261 catcgacatc ggtgcgtgca tacgtcggcg tgcagggcga tgcgcattac gtgttggccg
    16321 aaatgccggt cgcggccacg tcgaaaccgc gcatcatccc caagcacgtc gggttgcttt
    16381 gggatgcatc ggcgtccggt cgcaagcgcg acattgccgg ggagctggaa ctgctggatc
    16441 gctatttcaa ggcgatgggc gatggccgcg tccggctccg cgtgcttcgc gatcgcggca
    16501 cggaggccgg gtcgttcccg gtgcgcggcg gcgactggag cgagcttcgc aaggcgttgt
    16561 cggcggtgat ctacgacggc gccaccgacc tcgccgattg gcgtcccact cccgggatcg
    16621 gcgaatacct gctggtcagc gatggcctgc agaactacgg cacgcaagcg ataccgacga
    16681 tccaggcggg ccagcgcctg tacgcgttgg cgagcaccgc cgccgatgcc acccgccttg
    16741 cggcaatcgc agaagcacga gatggccgcc tggtggcatg gcagggacgt gccgggatcg
    16801 acgccgcggc ccgggcgctg ttggtggatg gcacgcatct ggtcgcgctg gaaggcgagg
    16861 gtgtcgatca actggtggcg cagtcgcgct gggtcgacgg cgacatcctg cgcgtggccg
    16921 ggcgcctgcg tgaacccaag ggccggatca tcgccagcgt cgaagatgcc aacgggcgtc
    16981 gccgcatcga ggtgccggtg gcgagtgccg gtatcgccca tgcgcaagtc ccgcaggcgt
    17041 gggccgcgtg gtcagtcgcg cggttgtccg gggagcccga acgcaaccgg gccgcgatca
    17101 cgcgattggg cacggaattt tcgctggtca cggcgggcac gtcgctgctg gtactggacg
    17161 atccggccga ctacgtccgc tacgacatcc cggcgccgcc ggcattgcag gcgcaggtgg
    17221 taacgatccg gagccagcaa caccgcgagc gcgaggcctc gcgcagcgag cagctcgacg
    17281 cattggccac ggaattcgcg ggtcgcgtcg cctggtggga gcgcgcgttt cccaagggcg
    17341 cgcgcgctgt cgcacaaccc aggaaggccg aggccggcgc cggcacgctg gacaccgtga
    17401 cggtgaccgg atcgacggtc cttggacgga atgcggcggc ttccatggcg ccaccgcccg
    17461 cgccgatcgc cgaagcgccg gctgcggatg ccacgacact cgataccgtc accgtcgcag
    17521 aatccggtgc catccatgca atcgatgttt cgtccgacga gagcacccgc attgccggga
    17581 atgtccaggc caccatccgc ctgcaaccct gggaaccgga ctcgccgtat gcccgccgcc
    17641 tgcgtgccgc acctgccgac cagctctatc cgctgtacct cgacgaacgc gacagccatg
    17701 ccgccagcac cgcgttctac ctggacgtcg ccgacctgct gctgcaacgc gagcgccgcg
    17761 acgaagccct gcgcgtgttg tccaacctcg ccgagctgga actggagaac cgccacgtcc
    17821 tgcgcgtgct gggctaccgg ttgatgcagg ccaaggcgta tccgctcgcg gtacaggtct
    17881 tccgccaggt gctggagatg gctgatgagg aaccgcagag ccatcgcgac cttgcgctgg
    17941 cgctggcggc cgatggcgag cggcaggagg cgatccgcca tctgtatgac gttgccaccc
    18001 gcgagtggga tggccgcttc gcggagatcg aactggtcgc attgaacgaa ctcaaccaga
    18061 tcgtcgccac tgcgcccgcg ccgctggaga ccggcttcat cgacccgcgc ctgctgcgca
    18121 acctgccgct ggacctgcgc gtcgcgctgt cgtgggacag cgacaacagc gacatggacc
    18181 tgtgggtgac cgacccgaac ggcgagaagt gcttctacgg caatacgctg acctaccagg
    18241 gcgggttgat ctcggaagat ttcaccggcg gctacggccc ggaagaattc gtgctgcgcg
    18301 atgccaagcc gggcaagtac cgggtggagg cgcactactt cggcgaccgc cagcaggtgg
    18361 tgaccggagc caccaccctg tcgctcaggc tcagcacggg ctggggaacg cgccagcagc
    18421 gcgaccaggc cgtcaccttg cggctgtccg gcaaggatga gtccgtgctg gtcggcgaat
    18481 tcgaagtccg atgagcgggc agcagccggg ccaggtcgcc cggctgctat gattcacggc
    18541 catttttccg ccgcctgcgc ctgcagggaa tgccaatgat cgagctcaat cccatccgag
    18601 cccgcatcgc cgacctgacc ggtcggctcg atgcgctccg ggggtatctt tgactacgac
    18661 gccaagcgtg aacgcctgga agaagtcgaa cgcgaactgg aaaacccgga catctggaac
    18721 gaccccgaac gcgcgcaggc gctgggcagg gagcgttcca tgctggacaa gaccgtcaac
    18781 ggcatccgcg acctgaagga cgggttgggc ggggcgggcg aactgctcga actggccgag
    18841 atggaggacg acgaggaaac cgcgctggcg gtggtcgccg acgtggaccg cttcgagaag
    18901 ggcgtggacc agctggaatt ccagcgcatg ttctccggca agatggattc ggcgaacgcc
    18961 ttcgtcgaca tccaggccgg tgcaggcggt accgaagcgc aggactgggc cgagatcctg
    19021 ctgcgcatgt acctgcgttg ggccgagaac cggggctgga agaccgagct gatggaagtc
    19081 agcggcggcg acgtggccgg catcaagtcg gcgacgttcc gggtcgaggg cgaatacgcc
    19141 tacggctggc tgaagaccga gatcggcgtg caccggctgg tgcgcaagtc gccgttcgac
    19201 tccgacaacc gccgccacac cagtttcacc agcgtattcg tgtcgccgga agtcgatgac
    19261 gacatcgaga tcgacatcaa tccggccgac ctgcgcaccg atgtgtatcg cagctccggc
    19321 gccggtggcc agcacgtcaa caagaccgag tcggcggtgc gcatcaccca catcccgacc
    19381 aacacggtcg tggcctgcca gaacggccgc agccagcacc agaaccgcga tactgcgatg
    19441 aagatgctgg cggccaagct gtacgaactc gaggtccaga agcgcaacgc cgagaaggac
    19501 gcgctggaag cgaccaagtc cgacatcggc tggggcagcc agatccgcaa ctacgtgctg
    19561 gaccagagcc ggatcaagga cctgcgcacc ggcgtggagc gcagcgacac ccagaaggtg
    19621 ctggacggcg acctcgacga attcgtggaa gcgagcctga agtcggggct ggaggctggg
    19681 tcgaaacggg tcgatgcggc ctgaccgcat ccaccttccg catcgccccc cggatcccat
    19741 acggcacgcc tccatgaccg acgaaaccca gacgccgcaa accgacgaaa accacctgat
    19801 cgccgagcgc cgcggcaagc ttgccgcgct gcgcacgcag ggcatcgcgt tcccgaacga
    19861 tgcgcgtcgc gcggacttcg caggcgacct gcaggccgac tacgccgacg tggaggcgtg
    19921 gaccgcggag gcgctggaag cgaccgggcg ccgcgtcgcc atcgccgggc gcctgctggc
    19981 caagcgggtg atgggcaagg ccgcgttcgc caccctgcag gacatgagcg gccgcatcca
    20041 gttgttcctg caatcgaccg cgctgggcga ggcctacgac gccttcaagg gctgggacat
    20101 cggcgacatc gtcgcggtcg agggtggcgt gacccgcacc cgcaccggcg agttgtcggt
    20161 gaaggtcgat gccctgcgcc tgctggtgaa gtcgctgcgg ccgctgccgg acaagttcca
    20221 tggcctggcc gacgtcgagc agcgctatcg ccagcgctac gtcgatttga tcgtcaatcc
    20281 cgaggcgcgc gacgtgttca tcgcgcgctc gcggatcgtc gcggcgatcc gtcgctggct
    20341 ggatgcgcgg cgcttcctcg aagtggagac gccgatgatg cattacatcg tcggcggcgc
    20401 caccgccaag ccgttcacca cccaccacaa cgcgctggac ctcgacctgt tcctgcgcgt
    20461 ggcgccggag ctctacctca agcgcctgac cgtgggcggg ctggaacgcg tctacgaaat
    20521 caaccgcaac ttccgcaacg aaggcgtcag tacccgccac aacccggaat tcaccatgct
    20581 cgagttgtac gaggcctacg ccacgtacat cgaggtgatg gacctcaccg aaggcatgat
    20641 ccgcgatgtc gcgcgcgaag cgatggccac gaccgtgttc gagtgggacg gcaaccgcat
    20701 cgacgtcgcc ccggcgttcc gacgctggaa gctggaagaa gcggtgcgcg aactcaaccc
    20761 ggagatctcg gtcgccgact gccgtgaccg cgacgcgttg gccgcgcatt gcgcgcgcct
    20821 gggcgtgcac gtgaaggccg gctatggctg gggcaagttg ctgctggaga tcttcgagaa
    20881 gaccgtcgaa gcgggcctgg tgcaaccgac tttcatcacc cactacccgg ttgaagtcag
    20941 cccgttggcg cgggaatccg acagcgagcc cggcatcacc gatcgcttcg agttgttcat
    21001 cggcggcaag gaactggcga acggcttctc cgagctcaat gacccggagg accaagccgc
    21061 gcgtttccgc gcccaggtcg aggccaagga cagcggcgac gacgaggcca tgcatttcga
    21121 tgccgactac atccgcgcgc tcgaggtcgg cctgccgccg acgggcggcc ttggcgtagg
    21181 catcgaccgg ctggtgatgt tgctcaccgg gtcgtcctcg atccgggacg tgctgctgtt
    21241 cccgtacatg cgcccggagc atgcaacggg ctgatgggga tggctggcct gcggcgccgg
    21301 ttggccgcgt tgtcactttc ggtatcgggg tgtgacgcag tgcgtcaccc tccgcgggat
    21361 ccccttgcca agtgccctgc attgcgcaga ggatgccgtg accgcgtaat cccctcattc
    21421 ccccgtgaca ggagcggcgc atggacatag tcatcgtcga cgaccagcct tccgcacgga
    21481 cgatgctgcg gcacatcgtg caggacatcg ggccgcagct cgacgtacac gatttcggcg
    21541 agcccgatgc ggcgctggag tggtgcaacg agaaccgccc cgacctgctt ctgctggact
    21601 accgcatgcc cggcatggac gggctggagc tggcgaaggc cttccgcagg ccgctgcgca
    21661 accgcgacgt gccgatcgtg ctggtcaccg tggtcggcga cgagccggtt cgccaggccg
    21721 cgctggatgc cggtgtgatc gatttcctgg tcaagccgat ccgcccgcgc gaactgcggg
    21781 cgcggtgccg caacctgttg cagctgcgcc agcagtcgga gtcggtgaaa cagcgtgcgc
    21841 tgtcgctgga gcaacgcctg ctggcgagca tgcacgaagt cgaggagcgc gagcgcgaga
    21901 ccctgtcgcg gctggcccgc gcgatcgagt tccgtgatgc cggcaccagc gcctacctgg
    21961 aacgcatggc ccacgtggcc ggcctggtcg cggaagcgat cggcctgccg gaagaacagg
    22021 cgcgcaccat cgaactggcc gctccgctgc atgacatcgg caagatcgcg atcccggact
    22081 cggtgctgat gaagcccggc ccgctggacg atgccgagcg ccggcgcatg caggaacatc
    22141 cgagcatcgg ctaccagctg ctgcaggaca gccagaaccg cttcatccag gtgggcgcca
    22201 agatcgcgtt gcaccaccac gagcgctacg acggcaccgg ttacccgctg ggactggcgg
    22261 gcgaggagat cccgatcgag gcgcgcgtgg tcaccgtcgc ggacgtcttc gatgcgctga
    22321 tttcgcggcg gccctacaag gacgcgtggc cgatcgagcg cgcgctcgag ttcatcgagg
    22381 gacagagcgg caagatgctc gatcccgttt gcgtgcgggc gttgctggac aacctgccgc
    22441 gcctgcacga catctgcgag cgctattcgc aagtggtgcc gcagccgggc atccactgac
    22501 ccatggacgg ggccgcgcgc cggatgctgt cccgcctgcg cggccgccag gacagcgagc
    22561 acgcgcaggt cttcgtccgc atcgtcatca ccacactctt cgcggcctac ctgggctggg
    22621 aggtcagcgg cggcgatggc aatcgcgccc tgttcctgac ctggtggatc ctgctcggcg
    22681 agtggctgct gtcggtggtc ctgctggcgg ccatcgtggc ctcgccggga ccatcggacc
    22741 tgcggcgctg gatcggcatg ctggccgact acgccgcgat gggcggggtc atgcacctgc
    22801 agggcgagac cgccgcgccg ctgtacgcgg tgttcctctg ggtgaccatc ggcaatggca
    22861 tgcgctacgg gccgggctac ctgttccgcg ccaccgccct ggccggcctg tccttcggcg
    22921 cgatgatgca ggccacgccg tactggcagg aaaacccgta cctgtcgtgg ggcctgctga
    22981 tgggcctggt ggcggtgccg ctgtacttcg cctcgctgct caaggcgttg accgccgcga
    23041 tcgaggatgc acgccgcgcg aaccaggcca agagccgatt cctggccaac atgagccacg
    23101 agttccgcac gcccttgaac gggttgtcgg gcacgtccga actgctggcc agcacgcgcc
    23161 tggacaacga gcaacgcggt taccttgaaa ccatccaggc tgcgacgcgc tcgttgctgt
    23221 cgctggtcga ggacgtgctg gacatctccg cgatcgaggc cggcaagctc aagctgaagc
    23281 tggaggcatt cgacctgcgc gagctggtcg agcagatcga cctgctgttg cgtcccgatg
    23341 cgcgcgcgcg ccatctcgac tacgtggtgg cggtcgcgcc cgacgtgcca gtcgccttgc
    23401 gcggcgatga aggccatctg cgccaggtgc tggtcaacct gctcagcaac gcactcaagt
    23461 tcacaccgtc cggcgaggta cgcctggcca tcgaagcggc gagccccgcg gtcgacggcc
    23521 gccagcgcct gcggttcacc gtgtcggata ccggcatcgg catcccggca tcggcacggg
    23581 ccaagttgtt cgaggccttc gaacaggccg acaacagcct gtcgcgccgg catgccggca
    23641 ccggccttgg caccaccatc gccaagggcc tgaccgaggc gatgggcggc gcgatcggat
    23701 tcgagagcag cgagaacatc ggcagtcgct tctgggtgga attgccgttc gagttgcagg
    23761 gggtggatgc ggcaccggcg gtgcctgtac gggagccggc aacgctggtc cccgcgccag
    23821 tccagggcga gggcggcaac gtcattgcct tcgccgatcc cttcctgcgc catcgcgcac
    23881 gggtcaagcc gatgcgcgtc ctgatcgccg acgaccatgc cgccaaccgg atggtgctgc
    23941 agggggtgtt gcagaaggca gggcaccggg tgatggcggt ggaggacggc gaggaagcac
    24001 tggatgcgct ggcggtcggc gacttcgaac tggccgtggt cgacctgcac atgccgtcgc
    24061 tgagcgggat cgacatgctg cgccagctgc gggtcatgga ggcgggtggg caggcacgga
    24121 cgccggtggt ggtgctcagc gccgatgcga ccccggaagc ggtccaagcc tgcaaggacg
    24181 ccggcgcgcg cgccttcatc gccaagccgt tcgcggtggc gaaactgctg gacgggatcg
    24241 ccgagatcgc attgggcgga tcgattggaa caccagtgga catcccgcgc gacaacgtgg
    24301 ctgcgaacaa cgacgacatc ctggatgcct cggtgctgga cgagttcctg gcgatcggca
    24361 tgggccctgc gttcgaggcc aatttcatcg gccagtgcgt ggcggatgcc aggatgatcc
    24421 tgacgcggat gcgccggtcg ggtgaagcag ccgactggga acaagtccac gagcaggcgc
    24481 atgcgatgaa gggcgtggcc ggcaacctgg gcctgttgca acttgccgcc cttgccgggc
    24541 aagtcatgcg gatgaccggc ttcgaactgg cgcgcgactg gcgccggcac tgcgacacgc
    24601 tgggcgagcg cctgcgccgt ggtgaactgg cgctggcggc gcgcgggaac tggcatcccg
    24661 cgcgcgagga cagtcagtag cgatcaggcg gtggcggcat ccaggtggcc gtggcgcttg
    24721 tactgcgcgc gcaccagccg ctccatcgtg cgcagcgact tctcgccgag ttgcagggct
    24781 gtgtccaccc agacttcggt gatcgccatc atctcttcca gggtgaccgg ctgcgccatc
    24841 gcccgcacgc ggtgcatcgc cgcgcgcgca tgcgggatgc ggcgattggc ccggatcacg
    24901 tcctcgacgg cctgcacgcc ctggccacgc ggcaccagca cgtcgaccag gcccatcttg
    24961 aagagctctt cgctggaatg gatctggccc gagagcatca gcttctccgc ctggtgcggg
    25021 gcgatgcgct tgcacaggaa cgagtacgca cccatgcccg ggaacaggtc gaacaggacc
    25081 tcaggcaggc ccatgcccac gccttcctcg gcgacgatgg tatggcagct caacgcggcc
    25141 tcgaatccac cgcccagcgc gtcgccctgg accagcgcga tgctgtgcgc gcccgcatcc
    25201 agcccggtgt ggaaggcatg tacgccgcgc acgcactggc gcgcgtaggt cagcagcgcg
    25261 tggcggtcct gccggcggat cgaatcgcag aaatattcca ggtcgccgcc caggttgaag
    25321 gcatcgcaat cggacgccag caccacgtgc tggagggtgc cggcctcgtt gccgcgcgtg
    25381 cggcgcatcc ggtcgccgag cgtccactgg tactggatga tctcgttgac caggttcggc
    25441 ttgaaacagg cgcggccggg gttgtccacc aggtcggcgt gcatgtggca ccaatgggtg
    25501 ccgtcggcgc tctcgacgac gcgcagggcg gagtaacggg aaacgggggt gagtttttcc
    25561 aggatggcca tgatcgctcc ggacggttgc cgtcggcccg ggccagccgg cggggcaggg
    25621 ggaatgtcaa gagttgaccc gtccggcctc tatgcgccga aaaacgtcga agtgcaacaa
    25681 aaaaagcccc gggcgtggca ccggggctcc gattcgtgac ggcgggcggc ttgtgtcagc
    25741 tcgcgctggg cgagcgcagt tccttgcgca ggatcttgcc gacattgctc ttcggcagct
    25801 cgtcgcggaa ctcgacgaac cgcggctgct tgtaaccggt gaggttttcg cggcagaacg
    25861 ccttcaccgc atcggcggtc agtgcgggat ccttcttcac cacgaatacc ttcaccgcct
    25921 cgccggactt gtcgtctggc acgccgatcg cggccacttc cagcaccgcc ggcatcatcg
    25981 cgatcacgtc ctcgacttcg ttcggataca cgttgaagcc ggacaccagg atcatgtcct
    26041 tcttgcgatc gacgatgtag aagtacccct gctcgtccat tcgcgccatg tcgccggtat
    26101 gcagccaccc gttggcatcc atgaccttgt cggtttcctc ctggcgctgc cagtagccct
    26161 tcatgacctg cgggcccttg acgcaaagct cgccgacttc gcccatcggc agcacgttcc
    26221 catcgtcatc cttgatgcag acgtgggtgg acggtaccgg cagtccgatc gagccgttgt
    26281 aggcgcgcag gtcgagcggg ttgatgcaca ccgcgggtga ggtctcggtc aggccatagg
    26341 cctcggcaag ggtcacgccg gtgacctgct tccacttgtc ggccaccgcg cgttgcacgg
    26401 ccatgccgcc gcccagggtg aggtgcaggc gcgagaagtc gacctggtcg aagccgggcg
    26461 tgttgagcag gccgttgaac agggtgttga cgccggtgat cgcggtgaac cgcaccgact
    26521 tgatctcctt cacgaagccc ggcatgtcgc gcggattggt gatcagcagg ttggctgcgc
    26581 cgaacttcat gaacaccagg cagttcgagg tcagcgcgaa gatgtggtac agcggcagcg
    26641 cggtgacgat gagttcctcg cccagcttga cgttggtgcc cacccacgat gccgcctgct
    26701 gcatgttggc cactaggttg cgatgggtca gcatcgcgcc cttggacacg ccggtggtgc
    26761 cgccggtgta ctgcaggaag gccaggtcct cgggcgtgtt gcggatcacc ggcaactggt
    26821 gcatgcggcc cagggtgagg gtgtcgcgga aacgcacggc gccggggata tcgaaatccg
    26881 gcaccatctt cttcacgtgc ttgagcacga agttgacgat caggcccttc gggaagccga
    26941 gcatgtcgcc gaggccggtg gtcaccgcgc gcacaccggg aacctgtacc aggacctcct
    27001 gggccacgtt gccgaagttg tccatcacca ccagcacgct ggcgccggag tccaccaact
    27061 ggtgcttgag ctcgcgtgcg gtgtacatcg ggttggtgtt gaccacggtc agcccggcgc
    27121 gcagcacgcc caggatcgcg accgggtact gcaggcagtt gggcatcatg atcgccacgc
    27181 gatcgccctt cttgagcttc agctcgccca gcaggtaacc ggcgaaatcc cggctcaggc
    27241 gttccagttc gccataggtg atcgacttgc ccatgctgat gaacgcggtc ttgtcgcggt
    27301 acttctgcac ggcggaatcg aagatgtcat tgatcgacgc gaactcgtcc aggtcgattt
    27361 cggcgggcac gccagcgggg tagtgggcca accaaggacg ttcggaactc atgcacgatt
    27421 cccctgcgag tgaacgccgt catattcgcc ggcgtatgga ttgcggtcga ttccagcata
    27481 ggctccaatc attgacaagg cgattcgttc gcatcggagc aatgccgcaa tgcagcaatt
    27541 ccgcgggttc cagcgcatcg cgaccgccct cgccatgact gcctgcctgt ggctgggcgc
    27601 atgcgccaag gacgatcccg agcaggcggt gcgccagcag gtcgaggcca tgcaggccgc
    27661 catcgatgcg cgcgatgccg gcgacgtgga agacctcatc gccgaggatt tcgtgggcaa
    27721 cgaggggatg gatcgccgcg ccatccgcca actggccgcc gcggtgttcc tgcgccacaa
    27781 gaccgtgtcg gccaaactgg gaccggtgag cgtggagttg cgtggcagca ccgatgcgat
    27841 cgcgcgcttc agcgtgctgg cgaccggcag ttccggtggc ttccttcccg aaagcggcca
    27901 ggtctaccag gtcgagtccg gctggcgcct ggtcgatggc gagtggaagc tgctcaatgc
    27961 cagctggacg cccaatctgt aagtccggcg ctcaagacgg cgcgcgttcg cgatgagccc
    28021 gtctggcgac atcaatccca gccacttgca actgccgccc ggcccctggg cgaccttgct
    28081 cgacgggtta tgcgcgcggt tcccgcggat cacgcgtgaa acgtggctcc agcgctttgc
    28141 gcgcggacgg gtgcaggacg cggacggaca ggcgctggcg gcggatgcgc gataccgggc
    28201 gggaaccgtg gtccgttact tccgcgaggt cgacgacgag ccgcggattc cgttcgaagc
    28261 gaagatcctg ttcgccgacg ccgacctggt ggtggcggac aagccgcact tcctgccggt
    28321 gacgccggcc gggcgtttcg tggacgaaac gttgctggca cggttgcggc agcaactgga
    28381 caacccggcg ctggtgccgt tgcaccgcat cgaccgcgaa accgcggggg tggtgatgtt
    28441 ctcggccaac ccggcgtctc gcgcggcgta tcacgcgttg ttccagcagc gggcggtgga
    28501 gaaggcctat gaggccatcg cgccgccatt gccggcgctg gcgatgccgc atgtgcgccg
    28561 ctcgcagttg cgcgcgggcg agccgttctt ccggatgcgc gagggcgacg gccccgtcaa
    28621 cagcgaaacg cggatcgagg tgatgcaacg gggactgcac gcatggcgct atggcctgca
    28681 tccggtcacc ggccgcaagc atcaattgcg cgtgcacatg gccgcgctgg gcgcgccaat
    28741 cgccaacgac agcctctatc cggcgatgtc gatgcggacg cccgatgatt tcggcaggcc
    28801 gttgcaactg ctggccaagg cgctgcgctt catcgatccg ctcgatggca cggcgcgcat
    28861 gttcgaaagc cgccaggcgc tcgtgctcgt ggacgcgtga cgctgcggcg cgccgtcagg
    28921 gccgggtgcg ggcccgaccg aacgtcatgc cgcttgcttg cccgccagtt gccgcagcac
    28981 gtattgcagc aggccaccgt gccggaagta gtcgacttcc ttcggcgtgg ccaacagcac
    29041 cctggcctgg aaggcgatcg tgctgccgcc ggctttcgtc gccgtcaccg tggcggtctt
    29101 cgacgcacca tcggccaggc cggtgatgga gaacgtttcc gaaccgtcca ggccgagttc
    29161 catggcgttg tcgccatcga tgaactgcag cggcagcacg cccatgccga ccaggttggc
    29221 gcgatggatg cgctcgaagc tctcggcgat gacggctttc acgccgagca ggttggtgcc
    29281 cttggccgcc cagtcgcgcg atgagccggt gccgtattcc ttgccggcga tcaccaccag
    29341 cggcacgccg tcggccttgt acttcatcgc tgcgtcgtag atcgccagct tctcaggctt
    29401 ggcgtcgctg ctgaaataca gggtgttgcc gccttcctcg ccgccgaaca tcaggttctt
    29461 gatgcggatg ttggcaaagg taccgcgcac catcacgtcg tcgttgccgc gacgcgagcc
    29521 gtagctgttg aagtccgccg gctgcacgcc gcgttcctgc aggaagcggc ccgccggcga
    29581 gctgcccttg atgttgccgg ctggcgagat gtggtcggtg gtgatcgaat cgccgaacaa
    29641 ccccagcacg cgcatgccat gcaggtcttc gatgtggccg acgtgcatgg tcatgccatc
    29701 gaagtagggc gggttcttga tgtaggtgga cgcggcatcc cactggaagg attcgccttc
    29761 cggcgacgcg atgccgttcc agcgcgaatc gcccttgaac acgtcggcat agttttgcgc
    29821 gaagagttca gggccgacag tcgccgcgat gctgtcgccg atttccttgt tgctcggcca
    29881 gatgtcgcgc aggtagacat cgttgccatc gccgtccttg ccgagcggat cgcgggtcag
    29941 gtcgatgtcg acggtgccgg cgatcgcata ggccaccacg agcggcggcg aggcgaggta
    30001 gttcgctttc acctcgggat gcacgcggcc ttcgaaattg cggttaccgg acaacaccga
    30061 agcaacgacc agttcgttct ctgcgatgcc cttgctcact tcgtccggca gcggtccgga
    30121 gttgccgatg caggtggtgc agccataacc gaccacgaag aaaccgagtt gctccaggtc
    30181 gtccagcacg ccggccttgc gcaggtagtc ggtcacgacc agcgagcccg ggcccagcga
    30241 ggtcttcacc cacggcgcgg ctttcagtcc tttcgcaacg gcattgcgcg ccagcaggcc
    30301 ggcaccgagc atcaccgccg ggttcgaggt gttggtgcac gaggtgatcg ccgcgatcac
    30361 cacggaaccg tcggtgagtt cggcatcgac gtcgcggatg cggatcatcg agcgcggcct
    30421 gaccgccagg tgctcggctt gcggttgagc gccgccttcg tccttgaagt cctgcacgac
    30481 cgggttgcgc gccttgcggt tggcggtcag cgggcccagg ttgtcggcga aattggcctg
    30541 cacgtcttcc agcagcacgc gatcctgcgg gcgcttgggg ccggccagcg agggccgcac
    30601 cgaggccatg tccaacgcca gcgtggcgga gtagttcgcg tgcggcgaca tcgcgtcgtg
    30661 ccacaggccc tgcgccttcg cgtaggcctc gaccagcgca atctgcgcct cgctgcggcc
    30721 ggacaggcgc aggtaggtga tggactcggc atcgatcggg aagatgccgc aggtcgcgcc
    30781 gtactccggt gccatgttgg cgatcgtggc gcgatcggcc aacggcaggt gctgcaggcc
    30841 gttgccgaag aactccacga acttgccgac cacgccgtgc ttgcgcagca tctgggtcac
    30901 ggtcaacacc aggtcggtgg cagtggcgcc ctcgggcagt gttccggtca gctggaaccc
    30961 gaccacctgc gggatcagca tcgaggaggg ctggcccagc atcgccgcct ccgcttcgat
    31021 cccgcccacg ccccagccca gcacgcccag gccgttgatc atcgtggtgt gcgaatcggt
    31081 gccgaacacg gtgtcgggga aggcccaggt gtcgccatcg atgtcgcgct ccaccaccac
    31141 gcgcgccagg tgctccaggt tcacctggtg gacgatgccg gtgttcggcg gcactacctt
    31201 gaagttgccg aaggccttct ggccccagcg caggaagctg tagcgttccg cgttgcgctg
    31261 gaattcgatc ttgccgttga ggtccagcgc gtcggcgcgg ccgaacacgt ccacctgcac
    31321 cgaatggtcg atgaccagtt ccgaggggat cagcggattg atctggctgg ccttgccgcc
    31381 gagcttcacc accgcgtcgc gcatcgccgc caggtcgacc acgcagggca cgccggtgaa
    31441 atcctgcagg accacgcggg cgggcatgaa ggcgatctcg gtggcgggtt cggcttgcgc
    31501 atcccagcgg gccagcgcct cgatgtggtc gcgccccacc gtgacgccgc cgtcctcgtg
    31561 gcgcagcagg ttctccagca ggatcttcag cgagtagggc aggcgggcga tgtcgaactg
    31621 ttcgcccagc ttcgccaggc tggcgtaggc atacgaggtg ccgttgacgt caaggcgggc
    31681 gcgggtggcg aaggagtcgg ccatgtgggg ctccgtggcg ggcgggggat cgggccagtg
    31741 tacgtgcggc gggatgaccg cgccgttgcg gtggcgccac ccgccgatcg acggccgggc
    31801 accggtacgc gaagagaagt atgtataatt tccgcatact ttttcgcgcg tggagctccc
    31861 gatggaagcc accgttgccg aacgcgggca gatcaccctg cccaagcccg tgcgtgatgc
    31921 gctgggcctg gccaagggca gcgtgctcaa ggtcgaactg gaaggcggtc gcatcgtcct
    31981 gagcaagcac gtggacgacg cgatctcgcg ggtccgcggc aagttcgcgc tggacctgcc
    32041 ggccggcggc aagggttgag ccgatgatcg cggtcgacgc gcccgtgctg gtcgaactgc
    32101 tgacggacgg cccgcgcgcg gatgccgccg aaacatccct gcgccaggca cttggcagtg
    32161 gccgggtggt ggtctgcgat gcagcgctgg ccggcgtctg cgcgtcgctt cgcaacggcg
    32221 cagatgcgct cagcgccctc gaggacatgg gcgtgcacta cagcgcggtc gaatccaagt
    32281 ccgcgctgcg tgccggcgag atgcaacgac gccagcgcca gcgcaacgac gagacgcgtc
    32341 cgctcgcgga cttcctggtc ggagcgcatg cactgctgca atgcgacgcg ctgatcacct
    32401 acgaaaccac ttttcatcgc gactacttca agggcctgaa gctgatcgtt ccgaaggcct
    32461 gatacctgat acccgttgca acttttcccg gagacgcccc catgctggaa gcctatcgcc
    32521 accacgccgc cgaacgcgcc gccctcggca tcccgccgct gccgctgagc gcgcaacaga
    32581 ccgccgaggt catcgacctg ctcaaggatc caccggcggg cgaggcgcaa ttcctcgttg
    32641 acctcatcac ccatcgcgtg ccggcgggcg tcgacgatgc ggccaaggtc aaggccagct
    32701 acctggcggc actcgcgttc ggcagcgaag cgaatccgct catttcccgg gcgcgtgcca
    32761 ccgaactgct cggcaccatg ctgggcggct acaacgtgca cccgctgatc gaattgctgg
    32821 acgatgccga ggtcggtgcc atcgccgccg aggcgttgaa gcacaccctg ctggtgttcg
    32881 atgccttcca cgacgtgcag gaaaaggcca acgccggcaa tgccaatgcg caggccgtcc
    32941 tgcagagctg ggccaacgcc gaatggttca ccagcaagcc ggaagtgccg gcatcgctga
    33001 ctatcaccgt gttcaaggtg ccgggcgaga ccaataccga cgacctgtcg ccggcacccg
    33061 acgcgaccac ccgcccggac atcccgctgc acgcgctggc gatgctgaag aactcgcgtc
    33121 ccgacgctcc gttcgcgccc gaggaagacg gcaagcgcgg cccgatcgcg cagatcctgg
    33181 cgctgaagga gaagggtcat ctggtggcct acgtcggcga cgtggtcggc accggttcgt
    33241 cgcgcaagtc ggcgaccaac agcgtgctct ggttcaccgg cgaggacatt cccttcatcc
    33301 cgaacaagcg cttcggcggt gtctgcctgg gctcgaagat cgcgccgatc ttctacaaca
    33361 cgatggaaga cgcgggcgcg ctgccgatcg aactcgacgt gtcgcagatg caccacggcg
    33421 acgtggtcga actgcgtccg tacgacggca aggcgctcaa ggacggcgcg gtgatcgccg
    33481 agttccaggt gaagtccgag gtgctgttcg acgaagtgcg tgccggtggc cgcatcccgc
    33541 tgatcatcgg ccgtggcctg accgccaagg cacgcgaggc gttgggcctg ccggcatcga
    33601 ccctgttccg cctcccgcag caaccggccg acaccggcaa gggcttcacc ctggcgcaga
    33661 agatggtggg gcgcgcctgc ggcctgccgg aaggccaggg cgtgcgcccg ggcacctact
    33721 gcgaaccgaa gatgacctcg gtgggctcgc aggacaccac cggcccgatg acccgcgacg
    33781 agctgaagga cctggcctgc ctgggcttca gcgccgacct ggtgatgcag tcgttctgcc
    33841 acaccgcggc ctatccgaag ccggtcgacg tgaagacgca ccacacgttg ccggacttca
    33901 tctccacccg cggcggcatt tcgttgcgtc cgggcgacgg catcatccac agctggctca
    33961 accgcatgct gttgccggat accgtgggca ccggcggtga ctcgcatacg cggttcccga
    34021 tcggcatttc gttcccggcc ggctctggcc tggtcgcgtt cgccgcggcc acgggcgtga
    34081 tgccgctgga catgccggag tcggtgctgg tgcgcttcaa gggcacgctg caaccgggcg
    34141 tgaccctgcg cgacctggtc cacgcgatcc cgctgtacgc catcaagtcc gggctgttga
    34201 ccgtggcgaa acagggcaag aagaacatct tctccggtcg catcctggag atcgaaggcc
    34261 tgccggacct gaagatcgag caggcgttcg aactggccga tgcctcggcg gaacgttcgg
    34321 ccgccggttg caccgtgcgc ctcaacgaag ccccgatcgt cgagtacctg accagcaata
    34381 tcaccctgct gaagtggatg atcgcggaag gctatgcgga cgcgcgttcg ctcgcccgtc
    34441 gcatcgccaa gatggaggcg tggttggcct cgccgcagct gttgcagccg gatgccgatg
    34501 ccgagtacgc cgcggtcatc gagatcgacc tggatgacgt ggtcgagccc atcgtgtgct
    34561 gccccaacga tccggacgac gccaagaccc tgtccgacgt ggccggcgcg accatcgatg
    34621 aagtcttcat cggctcgtgc atgaccaaca tcggccactt ccgcgcggcc gcgaagctgc
    34681 tggaaggcaa gcgcgacatc cccacgcgcc tgtgggtggc accgccgacc aagatggatg
    34741 ccgcggaact gaccaaggaa ggccactacg gcaccttcgg cgccgccggt gcgcgcatgg
    34801 agatgccggg ctgctcgctg tgcatgggca accaggcgca gatccgcgaa ggcgcgacgg
    34861 cgatgtccac gtcgacccgc aacttcccga accggctggg ccgcaacacc aacgtgtacc
    34921 tggggtcggc ggaactggcg gcgatctgct cgcggctggg gcgcatcccg acccgcgagg
    34981 agtacatggc cgacatcggc gtggtcaacg cgaatggcga gaagatctat cgctacatga
    35041 acttcgacaa gatcggcgag tacaaggacg tggcggacac cgtcgcggct tgagtgatct
    35101 caccccctcc ccttgtggga gaggggttcg aagaagcgcc cggcaatgcc gggcgttttc
    35161 atatgcatcg aagtgctggt cgtcgcgcat cgcccgacgg ctggcctcca accgcgaatg
    35221 tcctccgcca gcgatgaagc cgctggctcc cccttgattt cgcggttgga ggccagccat
    35281 caggcgatct tcatgtcgtg ttgtcggtct ggcagagcga gcctttccat cactcgtgtc
    35341 tgatggtcgg gcaggacgtt tggcagcggg gctattttgg cgaaatcaag gaggaggcag
    35401 cagctccatc gctgccgggg acattcgccg aaatagcccc gctgccgagc gacctgcatg
    35461 cgatagacaa ggagctcagc gcaacaacgc gtcgatcgcc gccgccatgt cgcgtcgctt
    35521 gaaggcgaaa tcccacaaac tgccaccgtt ctgccgccac aagaccgcgg agcgcagggc
    35581 agcaagcaac agcgcacgga cttcggcgac cacgccggct tgccccaaat aatgcgggtt
    35641 gccttgcacc atcacccgcg gacgcaaggt gcttacggtg tctgcgtaca ggctacccag
    35701 gccggcgatc acgtccggat gcgtgctgcc caggcgttcg gccggcgccg acaacgcgat
    35761 gatcccggtc tgcaccttgt ggacaatacc ggcatcgccc tggaaacggc gctccagttg
    35821 caataccgag agccctagct tcgccagtgt ttcatcgcgc tcgttgttgg ccaggtagtt
    35881 gtgcagcagg cgcagcccgg cattgaccgc gccggcatcg ccatacaccg cctttgtggt
    35941 ggcggcgtcg atgcggaaca cgctgtccag cgcgacctcc gcctgtgcgg tctccgactg
    36001 gccggtctcg gcgatccggc gcacctgcgc cagtgcctgc aacacgccgg ccagggccag
    36061 tacgcggtct tccatgtcgc tcatgcagcg tcgttcctcg tgagtcggtg cgggacggcg
    36121 ggcgcatccg tgtgcaggat gaccgcaccc cccaggcaca cgtcgccgtc gtagagcacc
    36181 agcgattgcc ctggcgtgac ggcgcgttgc gggcgcgcga agcgcacgct ggcactgccg
    36241 tcggcgagca cctggacatc gcagggttca tccggttgcc ggtagcgcgt ctgcgccgtg
    36301 cagttgaagc gtgaagccgg ggctgcgcct gcgatccagt gcatcgtctc cgtacgcagc
    36361 gactgcgact gcaaccacgg gctgtcgtgg ccctggtcga cgtagagcag gttgcgttcg
    36421 acatccttgc cgaccacgaa ccacggtgcc tgcgggcggc cacgcacgcc gccgatgtgc
    36481 aggccttcgc gctggccaag cgtgtagtag aagacgccag gatgttcgcc cacccggtgg
    36541 ccggcagggt cgcggatctc gccaggccgg gccggcatgt actggcccag gaactcgcgg
    36601 aaatcgcgtt cgccgatgaa acagataccg gttgaatctt ttttcgccgc ggtcggcaat
    36661 tgctgttcgg tcgcgatccg gcgcacctcg tccttgtgca actcgcccag cgggaagcgc
    36721 gttgccgcca gttgccgctg gcccagctgg tggaggaaat agctctggtc cttgccgcga
    36781 tccttcgccc gcagcaagcg atattcaccg gcgacgcagt cgacgcgggc gtaatggccg
    36841 gtggcaatgg catcagcgcc gaggtcgtgc gcggcgtcga ggaaatgctt gaacttgatt
    36901 tcgcggttgc acagcacgtc cgggttcggc gtgcggcccg cggcatattc ggcaagaaaa
    36961 tgctggaata cgccgtccca gtattcgcgg gagaaatcgc ggaagcggat cggcaggccc
    37021 agctttccgc tcaccgcgac cgcatcgcgc cggtcgtctt ctgcacggca atcgccggat
    37081 ccatcgtcgg cccagttctg catgaacagg ccggtaacat cgaagccggc atcgcgcagc
    37141 aacagggcgg ccacggaaga atccacgcct cccgacatgc cgacgacgat gcgacccgcg
    37201 ttcatgccag ttgccgcacc aggtccagcg gatgccggct gccgccgagg tagtcggcga
    37261 cgcagcgcca gacgagggga ctgcgatggc gcgatgccaa cgcctgcagc tcggacgggg
    37321 ccagccacag cgcctgcacg atgccttcgt ccagtcgggc atccggaacc tggtggatcg
    37381 gttcggcggc gaaggcgaag cgcaggtagt ggcggccggt ctccgccttc cactggtagg
    37441 caccgaccag atcggtcacc cgcacgttcc agccggtttc ttcgcgggtt tcgcgcaccg
    37501 cggcctcgat gaggctttca tccggctcca ggtgcccggc cggctggttg atgaccagtt
    37561 gcccgttggc gcgctcctcc acgcacagca ggcgaccgtc gcgcaccacc agcgtggcca
    37621 cggtcacgtc cggctgccag aaacggccct cgcgataact catctcagag gtcgtccttc
    37681 ccgggcgtca gttccttctc catgtcatcg gcggtgctgg ccaccgtgtt caccaacgct
    37741 tcgaagtggg aggcggaaat ccccgcaggc accttgccgc cgaaatagag gtttttcccc
    37801 gagatctccc aggccccgaa cttgtacgtg gcattttcgc cgagcagtcg cctggccagg
    37861 gcggggtcga tcgccccgtc tgccagcacg ctggcaggcg agaaaatctc gtaaagcgtc
    37921 acccccgcca gttcttccgt ggtcccggag acgtagacca gttgcgatcg cttgtccttg
    37981 gaccactcgt agacgatctt gtaatcgcca tcttcatcga ccttgaacgg gatgtcggcc
    38041 gccttgagca gggtttcgag gacgggatcg gctgcacgtg cgcccggggc ggcgagcacg
    38101 aggagggcag tcaaggcaag gcgggtggtg agcatcgcag ggctcctgag gatgcagcca
    38161 ttgtgccggc cggggcgggc cagcgtccac gccggggccg gcgcgcctcc gtataattcg
    38221 cccatgcccc agacgcccga caacccccat cacgagcacg accacggcgt cgtggtcgcg
    38281 cccgccaagc cggacaccgc gcgtccaccg ctgtattccg tgttcctgct gaacgacgat
    38341 tacaccccga tggatttcgt ggtcgacgtg ctgatgcgtt tcttcacgct cgacctggag
    38401 cgcgcgaccc aggtgatgtt gcacgtgcat acgcgcggtc gcggggtctg cggggtgttc
    38461 acccgcgaga tcgcggaatc caaggtggcg caggtgaacg agtatgcccg gctgaaccag
    38521 catcccctgc tgtgcacgat ggagcgcgcc tgatcccggg cggcacttgt acggcgggcg
    38581 aatgccccct acataggaac cagtcgttcc ggagggtttc gcccccatgt tcagcaagga
    38641 cctcgagcac agcatcggcc agtgctacaa gcgtgcacgc gatgcgcgcc acgaattcat
    38701 gaccgtcgag cacctggtac tggcactgct cgacaacgtg tccgcggagg ccgtgctcaa
    38761 ggcggtcaac gcggatatcc cgcgcctgcg ccacgagctg gaagaggcca tcgaggtctc
    38821 ggtcaaccgc ctggcggaag atgatggccg cgatacgcag cccacgctgg gcttccagcg
    38881 cgtgctgcag cgtgccgtgt accacgtgca gtcgtcgggc aagaaggaag tcaccggcgc
    38941 caacgtgctg gtcgcggtct tcggcgagaa ggactcgcat gccgtgtact tcctcaacca
    39001 gcaggacgtg caccggctgg atgtcgtcaa ctacatttcg catggcgtgg cccgccaggg
    39061 cgatgagcaa gccccgcaat cgcaggacgg cgagggcaag gcggagggcg gcgagggcga
    39121 atccaagagc gatccgctgg ccgaattcac cagcaacctc aacgacctgg cgctggccgg
    39181 caagatcgac ccgctggtgg gccgctccga ggaagtggaa cgcaccatcc aggtgctgtg
    39241 ccgccggcgc aagaacaatc cgctgtacgt gggcgaggcc ggcgtgggca agactgcgct
    39301 ggcggaaggc ctggccaagc gcatcgtcga gggcgacgtg cccgaagtac tggtcgaggc
    39361 ggtgatctat tcgctcgacc tcggcgcgct ggtagccggc accaagtatc gcggcgactt
    39421 cgagaagcgc ctgaagggcg tcctggcggc gctgaagaag ctgccgcacg cgatcctgtt
    39481 catcgacgag atccacacca tcatcggcgc gggctccgcg agtggcggga cgatggatgc
    39541 ctccaacctg atcaagccgg tgttggcgaa tggcgagctg cgctgcatcg gttcgaccac
    39601 gttccaggaa taccgcggca tcttcgagaa ggatcgtgcg ttggcgcggc gcttccagaa
    39661 gatcgacatc gtcgaaccca cggtgggcga ggccatcgag atcctcaagg gcctgaagcc
    39721 caaatacgag gcgcaccacg gcgtcagcta tgccgatgac gcgatcaagg cggcggtgga
    39781 cctgtcggtg aagcacatcg gcgaccgcct gctgccggac aaggccatcg acgtgatcga
    39841 cgaggccggc gcgcgccagc gcctgatgcc gatggacgcc cggaagagcc tgatcgatgt
    39901 cgaggaaatc gagaccatcg tcgccaagat ggcgcggatc ccggccaagc aggtcagcag
    39961 ttccgacaag gacgtgctgc agcacctgga tcgcaacctg aagatggtga tcttcggcca
    40021 ggatccggcg atcaccagcc tgacctcggc gatcaagctg gcgcgttccg gcctgggaaa
    40081 tcccgacaag ccgatcggca acttcctgtt cgccggcccc accggcgtgg gcaagaccga
    40141 ggtcaccaag caactggcca tgcaactcgg catcgagctg gtgcgcttcg acatgtccga
    40201 gtacatggaa tcgcattcgg tcagtcgcct gatcggcgcg ccgccgggct atgtcggctt
    40261 cgaccagggc ggcctgctga ccgagaagat cgtcaagacc ccgcactgcg tgctgctgct
    40321 cgacgagatc gaaaaggcgc accccgacat cttcaacatc ctgttgcagg tgatggaccg
    40381 cggcgtgctg accgatacca atggccgcga ggcgaacttc cgcaacgtga tcctggtgat
    40441 gaccaccaat gccggagcta cccaggccgc gcggcgcagc atcgggttca ccaagcagga
    40501 ccacagcacc gatgcgatgg aagtgatccg tcgcggcttc acgccggagt tccgcaaccg
    40561 cctggacgcg atcgtgcagt tccaggcgct cggtttcgac cacatcctgc gggtcgtgga
    40621 caagttcctg atcgaactgg aaatgctgct gcacgagaag caggtcagcc tgtcggccac
    40681 gccggccgcg cgcgactggc tggcccagca tggcttcgac ccgatgatgg gcgctcgccc
    40741 gatggcgcgg ctgatccagg accggatcaa gcgtccgctg gcggacgaac tgctgttcgg
    40801 caagctggtc gcgggtggcc gcgtgtcgct ggatgcggtc gatggcgagc tggcgctgga
    40861 cgtgcaggcc gaaccggaga agctgctgcc ggcagtggtc gagtaaaacg atccaggcaa
    40921 caaaaaaggc ggccattggc cgcctttttc ttgccgccaa tcaacgatgg cggtacgtga
    40981 tgcgtccctt ggtcaggtcg tacggggtca tttccacctt gacccggtcg cccgtgagga
    41041 tgcggatgta gttcttgcgc atgcgtccgg agatgtgcgc gatgatctcg tggccgttct
    41101 ccagcttgac ccggaacatg gtgttcggga gggtctcgga gatcgtgcct tcaaattcga
    41161 tgacgtcgtc ttttgccact gtgggtggta ttcctgttga agcgtgaggt tgctggctcg
    41221 gagtcggggc cgcgccgcga cccgaaagcg cggcattctg acacgtggcg gcggggcttg
    41281 caaaaatccc gttaaacctg tgcgggcctg gcgagcgatg aagcgtggat ttcgccgaaa
    41341 tcccgggtcc acgtccccgg caatccatcg ctgtcccgca atgcctccag cgcctgcagg
    41401 taggcggtcc tcggccaggt ttcaacgcca agtcggcgcg tgtgcccgtt cgggacctgc
    41461 gcgtcgatca acggccagcc ccaggcgtgc aggcgcatgg cgagggtcgc cagggcgacg
    41521 gtcgaggccc cggtttgcgc ggaatacatg ctttcgccgc agaacatccg gccgatggcc
    41581 agcccgtaca gtccgccgac gagggccccg ccatcgaaca cttcgatgct gtgcgcatgg
    41641 ccgaggcggt gcagttccac ataggcctcg accatcgcgc cggtgatcca cgtcccgcca
    41701 tcctggccag gccggggcag gtgcgcgcag gcctcgatga ccctgcggaa acaggtgtcg
    41761 gcgcgcacga ccaacgcgga cttgcgcaac tcacggcgac gtcgcgacga cagatggacg
    41821 ccgtcggtac gaaataccgc gcgtcgcgac gggctccacc acaggggcgg ctcatcctcc
    41881 gagaaccacg ggaagatgcc ctgacgatac gcgttgagca ggcgtgtcgg cgacaggtcg
    41941 ccgccgaagg cgagcaggcc gtccgggtcc gccagggcct gcatgaccgg cgggaaggat
    42001 gcccgtgggt cgtcatccag ttggacgatg cgcaggttca ttgcgccggc gccctgaacg
    42061 gcgaatgcgc ctgcagttgc cgcaggtgct cgcgggtcgc catccgttcc tcggcgcacc
    42121 acgcaccgat cgcctcggcg aagcgcggat cggcgatgcg gtggcggctg cgcacgatgc
    42181 tgggcaggaa gccgcgggcg atcttgtgct ggccctgcgc gccgggctcg aagcgggcga
    42241 ggccttcgcg caggcagtac tcgatcccct ggtagtagca ggcctcgaaa tgcaggccgg
    42301 gatgcagttc gcctgcgccc caatagcgtc cgtaaagggt gtcagcgccg cgcaggcaca
    42361 gggcgccggc gatcggcttc tcctcgcgct cggccagcac gatcaccagt tgcctcggca
    42421 attcccgggc aagatggcgg atgaaatcca gcgtcagggc cggcgaattg ccgtattcgg
    42481 cgaaggtttg cagatagaaa ccatgcatcg cggcgaagtc gccatcggac gcttcgccac
    42541 cgtgcacgat gcggaaggtg atgccggcct cgcgcacctt gcggcgttcc tggcggatgt
    42601 tcttgcgatg cttgtggtcc atcgcggcca ggaagctttc gaagtcagcc cagcccgcag
    42661 cgttatgcca gtggtactgg atgtcctcgc gcaacagcca ggcggcgtcg aatgcctttt
    42721 cgtcggtcgc atcgtggaaa ttcacgtgcg ccgacgacag cccggactgc tccaggaagc
    42781 cgccgatcgc atccagcaag gcgcgcttcg acgcctcgtc gcgtgcgagc aggcgcggcc
    42841 cggtcacggg cgagtagggc accgcgccga gccacttcgg gaagtagtcc agtccatgtc
    42901 gtgcataggc atgcgcccat gcatggtcga acacgaattc gccatgcgag ttggacttga
    42961 ggtaaccggg ggccgccgcg accagccggt cgccttgcca cagacccagg tggcacggcg
    43021 tccagcccca gtcctcgcgc aagcaacccg tggtttccag cccggaaata aaggcatgcg
    43081 ccacgaacgg attgcgcccg tcgtgcagcg cgtcccaatc ggcggcgagg atctccgaca
    43141 gcgcgcgatg gatgcgcgct gacggcatca gtgggcttcg tccaggaact tctcggcgtc
    43201 cagcgcggcc atgcagccga agccggcgga ggtgatggcc tggcgataga cctggtcggc
    43261 gacgtcgcca gccgcgaaca cgcccggcac gctggtctgg gtggcattgc cttccaggcc
    43321 ggtctggatg ctgaggtagc cgttcttcat cgccagctgg ccgtcgaaca acgaggtgtt
    43381 cggcgagtgg ccgatggcga cgaagaaacc gtgcacagca atctcgcggg tgctgccgtc
    43441 gagcatcgac ttcacccgca tgccggtgac gccggcatcg ttgcccagca cctcatcgac
    43501 cgtgtggtgc cagaccggct cgatcttgcc ggcctcgatc ttggcgaaca gcttgtcctg
    43561 catgatcttc tcggcgcgca gggtgtcgcg gcgatgcacc aggtagacct tgcgggcgat
    43621 gttggacagg tacagcgctt cctcgaccgc ggtattgccg ccgccgatca ccgccacgtc
    43681 ctggtccttg tagaagaagc cgtcgcaggt ggcgcaggcg gagaccccgc ggcccttgaa
    43741 ggcctcctcg gacgggatgc ccaggtactt cgcggtggcg ccggtggcga tgatcagcgc
    43801 atcgcaggtg tactcgccgg agtcgcccac gagccggaac ggccgcttgg acaggtcggc
    43861 ggtatggatg tggtcgaaca cggtctcggt gtcgaagcgc tctgcgtgcg cctgcatgcg
    43921 cgccatcagg tccggcccca tcaggccgtg ggcatcgccc ggccagttgt cgacctcggt
    43981 ggtggtcatc agctggccgc ccatggccat gccggtgatc acgacaggct tgaggttggc
    44041 gcgcgcggca tacacggcgg cggtccagcc ggcggggcca gagccgagga tcaggaggcg
    44101 ggtgtgcttg gtcggagtca tcgggatcca atggaaagcg tgatgcgcgg gtcgaaggac
    44161 ccgaggccga tgcatagctt ggaggcgggc tcgccatggc acaaggcgcg catgtagcaa
    44221 tgctgtcgac catggcagtg actttgtcat cgcatatgcg cgcggatcgc cccaggaagg
    44281 ttcccggata gacacgcctg caagagaagc gccagcctgc atgcgctgag cgctggatga
    44341 aactttgacg tattatcaaa ggcctagggg aacttcctga ggcctgatca caggtggcac
    44401 gcacggcgtc cgagaccggc aagagcagtc gcaaaccggc aacgaaatcc gcaacaccgc
    44461 gtgcggcgcc caacccgcgc cggcaacgcc tgtggcgcga ccttgcattg atcgccgtgg
    44521 cgccgttgct gttgttcctg ctcgccagcc tggtgacgta ttcgcccgcg gatcccggct
    44581 ggtcacattc cggcagcatc accgcgccgc tacacaatgc ctgcggtcgc gtgggggcct
    44641 ggatcgccga cgtattgctc tacctgaccg gctacgtggc cttcctgctg ccgatcgtgc
    44701 tcggcgcgat cgcctggatc gccctgttcg gcatggacag cgatggtgac ggcgatgccg
    44761 acctcgggcc tgcgctgcgc ctggtcggca tcgtcggctt cctggtcgcg agctgcggcc
    44821 tgctgtacct gcggatcagc ggatcgaacg atttttcggc cggcagcggc gggatccttg
    44881 gccagttgac cgggcgctca ttgctgcgcg gtttcgggcc agtgggcgcc aacctgttcc
    44941 tggtcagcct gttcctgata tcggtcacgc tggccaccgg gctgtcctgg ctggcggtga
    45001 tggaccggat cggcgccttc gcgctgtcgc tgcccggcct gctgacccgt ggcgcacgca
    45061 agggcgcgca gcaggccacc gactggagcg aggcccgcgc cttccgcgag gagcgcgtcg
    45121 aggcccgcaa ggtcgacagc gaattgcgcg ccaagcgcga gaaggtccgc atcgaaccgc
    45181 cgcccgcgcc ggtggtggaa aaaagcgagc gcgccaagcg cgagcagcag atcccgctgt
    45241 tccatgtcgc cgatggctcc ggcatcccac cgctggccct gctcgacgat cccaagccgc
    45301 agcccaaggg ctacagcgag gaaaccctgg aaacgctgtc gcggcagatc gaattcaagc
    45361 tcaaggactt tcgcatcgat gccgaagtcg ttggcgccta tcccggcccg gtgatcaccc
    45421 gtttcgaagt gcagccggcg ccgggcgtga aggtcagcca gatctcgtcg ctggacaagg
    45481 acatcgcccg cggactgagc gtgaagtcgg tgcgcgtggt cgacgtgatc cccggcaaat
    45541 cggtgatcgg cctggaaacc ccgaacaccc atcgagagat gatctatctc agcgaactgc
    45601 tgcgatcgaa ggaatacgac aagtcggcca gcccgctcac attggcgttg ggcaaggaca
    45661 tcgccggccg gccaaccgtg gccgacctgg cgcggatgcc gcacctgctg gtcgccggca
    45721 ccaccggctc gggcaagtcg gtcgcggtca acgcgatggt cctgtcgctg ctctacaagg
    45781 cctccgcgaa ggaactgcgg atgctgatga tcgacccgaa gatgctggag ctcagcgtct
    45841 accagggcat cccgcacctg ctggcgccgg tggtcaccga catgaaggag gccggcaacg
    45901 gcctgcgctg gtgcgtggcg gaaatggagc gtcgctacaa gctgatgagc gcggtcggcg
    45961 tgcgcaacct ggccggtttc aacaagaagg tgaaggacgc gctggacagc ggccagccga
    46021 tgatggaccc gctgttcaag ccgaatcccg acctggacga agtgccgcgc cagctcgaga
    46081 cgctgccgtt catcgtcatc ttcatcgacg aattcgccga catgatgatg atcgtcggca
    46141 agaaggtcga ggaactgatc gcgcgattgg cgcagaaggc gcgcgcggcc ggcatccacc
    46201 tgatcctggc cacgcagcgg ccgtcggtcg atgtcatcac cggcctgatc aaggccaaca
    46261 tcccgacccg catcgcgttc caggtgtcca gcaagatcga cagccgcacc atcctcgacc
    46321 agagcggtgc ggaaaccctg ctgggccatg gcgacatgct gtacctgccg cccggcactg
    46381 cgatgccgga ccgcgtccac ggcgccttcg tcagcgacga ggaagtgcat cgcgtggtcg
    46441 agcacctcaa gcagagcggc gggccggatt acatcgaggg cgtactggaa gaatcgcaga
    46501 cgatgtacga cggcagcagc gtcggcgcga ccggcctgcc ggaggccagc agcggaggcg
    46561 gcgacgagtc cgacccgctg tacgacgagg cggtgaagat cgtcaccgag acccggcgcg
    46621 cctcgatttc cggtgtgcag cgccggctca agatcggcta caaccgtgct gcgcggctgg
    46681 tcgaggcgat ggaagcggcc ggggtggtca gcacgcccga gcacaatggc gaccgcaccg
    46741 tgatcgcgcc gccgccaccg cgcccctgag tcgcgcgcag gcgtcgtgca ggcgcattca
    46801 gcttggcccg gacacactgc gacccagtct gcaggagaga tcccatgagc acttttggca
    46861 tgaagcccgc caccaccatc ctgtccgccg cgctgctgct ggccgccaat atcgccaacg
    46921 caagcgcgcg cgacgagctc aacgcgttca ccaaggggct gaaggggctg gatggacagt
    46981 tcacccagca ggtcttcgat gcgcgcggca agcagaagga aacttccagc ggccgtgtgg
    47041 ccgtctcggc accgcggctg ttccgctggg aatacgtcag gccgtatccg caattgatcg
    47101 tggccgatgg caagaccgtg tgggtgcatg accccgacct gcagcaggtg agccggcgcg
    47161 cccaaggcgt ggaagaagcc aacagccccc tggcgatcct gctggatccg tcgaagctgg
    47221 accgcgattt cgtggtgaag gatgccggca agacgggcgg catcgaatgg ctggaactca
    47281 cgccgaagca ggccgaggca ccgttcaagt cggcgcggct tggctttgcc aacaagggcc
    47341 tggcgcagat ggagtacgtg gatgcgctcg gccagcgcac gcgcatcagc ttcaccggct
    47401 ggaagcgcaa cccggcgttt gcgcggggca ccttcgtcta tgtgccgcag aaaggcgtcg
    47461 acgtcatcgg cggttgatcg catcccgcgg ttcgccctcg attcaggctg gcgcggtcca
    47521 cctcacgctg gctccacgtg gcgatgcgca cattgacgca acgccaccaa cggaagcctc
    47581 gccatggatc gcaagggact caaccgcacc gccattgccg tcgcgctggc actcgtgacg
    47641 gcaggtgcgg tcgttgcgca ggctggaacc ccggctacgc cgaacgccgt ggtggtcgag
    47701 ggacacagca acgatctcgt agcggccggc aatgccctcg attcacggat cctcgcgtcg
    47761 atcgacgacg ccaccgccgc tgcattgata ggcgccctcg aaacccgctt tgccggacgc
    47821 gaggtggcgc tgcggatggg cgaggtcgaa agcacgcgcg ccagcctccg cgatatcgcc
    47881 ctgcatggca ccgcgcagat ccgcctcggg caggatgcgg ggtggatgcc gatccggttc
    47941 gaagcgctct acgacaccga tacccaggcc gtgttgagcc ctgcgatcgt gctggaaggc
    48001 tcggccgtgg ccgctgcgcc caagctgcca ttgcccggcc tgcaggcaca ggttcgggct
    48061 gccatgggcg atgagttcgc ttcgcaacgg gtggatgtgt ccctgcgaca ggcgcaggtg
    48121 atcggcaatg atggccgccg gctggtggtg cgtgcgcaag gcatcgcccg gttcgacggc
    48181 agcgacaccg ccccggtgac cgtgcgcgcc ctgtacgagc agggcagcgg tcgctggctg
    48241 gacgcgcagt acgatttcga cgtggtgggt ggctgagtcc acaaggactt ggcgccgccg
    48301 gcgagacggc gccgcgtaga atcgacgggt ggccaagcct gcgaaaacca agaccatcga
    48361 tgccatgagc ctgctgcctg aggaaagcgg tagcgcgtcg tccccggacg tgtcgtctcc
    48421 cgacgtggtg gcgatgcggc ccttggccga acgcatgcgg ccgcggtccc tggacgacat
    48481 ggtgggacag cgtcgcctgc tggcgccggc ctccgcgcta cggcgtgcgg tcgaggccgg
    48541 gcgcgtgcat tcgatgatcc tgtggggacc gccggggtgc ggcaagacca cgcttgcatt
    48601 gttgctggcg cgctatgccg atgcggagtt cgtggcgatc tcggcggtgc tgtccggcct
    48661 gccgcaggtg cgcgaggtgc tggcggaggc gcaggcgcgc ttcgaaggcg gccgccgcac
    48721 ggtgctgttc gtggacgagg tgcaccgctt caacaaggcg cagcaggatg cgttcctgcc
    48781 gcacatcgag cgcggcagca tcctgttcgt cggcgcgacc accgagaatc cctcgttcga
    48841 actcaattcg gcgttgttgt cgcgctgccg cgtgcacgtg ctggaagcgg tgtcgaccga
    48901 tgacatcgtc gtcgcgctgc ggcatgcact ggatgatgcc gagcgcggat tgggcggccg
    48961 cggcctggct gtcgcggaaa acgcactgcg cgagatcgcg ggcgcagccg atggcgatgt
    49021 gcggcgtgcg ctgaccctgc tcgagatcgc cgcggacatc gccgatggcg aaggcggcac
    49081 gatcagcggg gccaccctgc accaggtgct ggccgaccgc acgcgccgat tcgacaaggg
    49141 cggcgaccag ttctacgacc agatctcggc cctgcacaag tgcgtgcgca gttccaaccc
    49201 cgatgccgcg ttgtactggc tggcgcggat gctggacggc ggcgccgacc ctgcttacat
    49261 ggcgcgcagg ttgacccgga tggcggtgga ggacatcggc ctggccgatc cgcgcgcatt
    49321 gcagatggcg atcgaggcat gggacgccta cgaccggctg ggttcacccg agggcgaact
    49381 cgcactggcg cagctcacga tctacctggc cagcaccgcc aagtcgaacg ccggctatcg
    49441 cgcgtggaac gccgctcgcg gcgatgtcga tgcactgggc acgcaggacg tgccactaca
    49501 tttccgcaat gcgcccacga aactcatgaa gtcgctgggc tacggcaagg actaccagta
    49561 cgaccacgat gccgacggcg gcatcgcgct ggaccagacc ggcttcccgg atgcgatggg
    49621 cgagcgcgtg tactacgcac cgaccccgcg cggcatggaa ggcaagctca aggaaaagct
    49681 cgatgccttg cgcgctgcgc gccagcaggc tcgcgacgcc aagggatccc gatgaacgcg
    49741 tggtggcagc aactcgtgct ggtgatgctg ggcggcgcgt tgggcgcagc cggtcgcttc
    49801 tggctgggcg gcgcgctgct gcggcaattc gggagcggaa tcccgtgggg aacgctggcg
    49861 gccaacctga tcggctcgtt cgccgtgggt tacatcgcgg tgtggctgga agggcgcggg
    49921 ccagcggcgc tgtactggcg tgcgttcctg atcgtcggct tgctgggcgg gttgaccacg
    49981 ttctcggcgt tgatgctgga gtcgttgctg ttggcgcgca cgcatcgcaa tgacctcctg
    50041 ctcgcctacc ttggcggcac gctggtcgcg gggctgctgc tggtatgggc aggcgctcgg
    50101 ctggccgagg cactgcgcgc tgcgtcgccc tgacccggtg catcgttcct actggtggac
    50161 ggcgtagggc ccgaacgtat tgccgatgaa accgccggcg tggtcgacgc tgagcaggct
    50221 ggcatccagc cgatttagca aggtgttcca gtgctccgtt ccgctgcgcc aggacacgtc
    50281 gaggtgctgg tcgtccagtt ggagccgcag cgataacgcc gcgtcgttcg atgccagtgg
    50341 cacccgcttc agttcgcgcc cgggcagcgg atcgtccttg ccattgcgca gacgaaggac
    50401 cagcgccgcg ccggcatcgc tgcgttcgac tgccgcgacg acgtggtgcg tctcgttctg
    50461 gaacaacgcc agtccggcca gctcgccgac ctccagaccc gccggctcaa gcgtcgcttc
    50521 gatccacgct ttgtggtgtt gcaggcgatg cgcgagatac gtcggctgac cgttgcctgc
    50581 ggcgccgtgc gcacccagcg aggtcgtgcc gggcacgatg cgcagaccgc catcgccggt
    50641 gcggaaccac ggcagttgcg gcgcatgcag ggtcaaccat tgcggaccgg gtgagtccgc
    50701 gaaggtttcg ctccacgtga tcgggcctga catcggaggc ggcgatggat cgcgtggcag
    50761 ggccgggcgc tgcgcgaccg cgggcacgcg cgcaccgcgc ggcagcacca tcggccagcc
    50821 atcgtccgtc cagctgaccg gcagcaggaa ggtttcgcgc cccaggttgt actggttgcc
    50881 gcgatacggg cgcgtgccca ggaacacggt ccaccaggaa ccatctttca gctcgatgaa
    50941 ctgcgcatgc ccggttgacg tgatcggaga ggggcggttc ggatcgaggt cgcgctgggt
    51001 cagcgaggga ttgccggtcc acgccacgta aggtccttct acgtcgcggc tgcgccagac
    51061 gcatttcgca taatgcctac cgggtcccgg ttgacccgag ccggtaagca cggcaagcgc
    51121 ccacgcatca ggcgcattac cccagaacag ttccgcgaga cacgcgagtt caccggcaag
    51181 agccgagagg acgttgcgga gttcctcggt gtcagccttc gcacggtggg tcattgggag
    51241 accggcaagg cacgggttcc atacgcggct ttccggctgc tccgggtcgc tctgaggggc
    51301 gacatgctcg acccggcgtg gcagggctac cgcatcgccc gcggccgact ggtgacgcct
    51361 gagggctacg ccttcgggcc gggcgacatg gcatggctgt cgctgctggt cgctcgtgcc
    51421 ggcttccaga gcttggttgc cattgcgcag cgagcggccc tgcaaagcgg gggctgctcg
    51481 cgttcagttg acgggcaggg cgagtcagtg ggagggtggg attctctggt gccgtgcgtt
    51541 gaggccggcg ccagttccgg caagagtccg ggtttaggac tcccctcgtc taacagggga
    51601 gtcagtgaaa ctgaacggga agcccgccaa gggcatgaaa cccgcaccac gactgcattt
    51661 gagcttaatt cggctcgttc agcatcgccg gtgcacgtgg ggccacaatg gggccataat
    51721 ggggccatcc aatctacggg
//
LOCUS       DAOOCX010000003        43974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_616_length_43974_cov_9.120426, whole genome shotgun sequence.
ACCESSION   DAOOCX010000003 DAOOCX010000000
VERSION     DAOOCX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 43974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43974
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_616_length_43974_cov_9.120426"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PK399_00505"
     CDS             <1..295
                     /locus_tag="PK399_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPW11641.1"
                     /translation="LPFAAPDDPVFEARLDAIRRAGGNPFRDEQLPQAVIALKQGAGR
                     LIRTETDRGVLVLCDPRLTQKSYGKVFMDSLPPLPITREVGDVQAFFRSQSTP"
     gene            292..996
                     /gene="tsaB"
                     /locus_tag="PK399_00510"
     CDS             292..996
                     /gene="tsaB"
                     /locus_tag="PK399_00510"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPW11642.1"
                     /translation="MRLLAFETATEACSVAVYVDGEICERFDLAPRRHAELALPWAGA
                     MLAEAGIARSQLDAIAVGRGPGAFTGVRLAIAIAQGIALALDRPVLPVSTLAALAMRA
                     GASNEGDRILAAIDARMGEVYLGAFARRGDDLVALDTEVVVKPEGASIPDGDGWHGVG
                     TGFAAAGNALPLRLEGRLAAVDATALPHAADVARLAALAYARGEAVAAERIEPAYLRN
                     NVALTLAEQQALRAKG"
     gene            1087..1551
                     /locus_tag="PK399_00515"
     CDS             1087..1551
                     /locus_tag="PK399_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11643.1"
                     /translation="MAGMLVAMPALAEDVVFEGHAVEVDATSEARPILGMRGTRYSIP
                     GSVSQIVGKAEQCATRQPGALTVESVEADAGRLVAGGRAEYRQKGRRSVRARMTLEAG
                     EGTFRVVFNDLATASADAGGFAPLIQQDAAGWENALEALIAIEQPLLDCMFR"
     gene            complement(1570..2460)
                     /locus_tag="PK399_00520"
     CDS             complement(1570..2460)
                     /locus_tag="PK399_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW11644.1"
                     /translation="MGAAAAPASLPAPPVIGESTRLGATLVLSVLVHAMVILGIGFAV
                     EDAAPMLPTLDVILSRTQTPLTPAQADFLAQANNQGGGEHDKSSRPTAPQAGPLPQPE
                     DGLAPRPLHAQSPAPTPPPDARVVASANGQQALPMPQPLQQVDANQAPRGREKIDRDI
                     EMARLAAEIQLRSQEYAKRPKRKFVSASTKEYAWADYLRGWVDRVERVGNLNYPDEAR
                     RRRIGGLVVISVGVRRDGSVESIRIVQSSHVPMLDEAATRIVKLSQPFSPLPKTQEDP
                     DVLHVTRTWQFLPGGELIDR"
     gene            complement(2460..3422)
                     /gene="gshB"
                     /locus_tag="PK399_00525"
     CDS             complement(2460..3422)
                     /gene="gshB"
                     /locus_tag="PK399_00525"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HPW11645.1"
                     /translation="MTDAAPLDVVVVMDPIESIKTTKDSSFAMLLEAQRRGHRLHYVR
                     PGGLALRGARAVATAAALTVQDVQGRHFRLGDWRELDFGPGQVVLMRKDPPVDADYLH
                     DTQLLGMAQRQGARVVNDPQGLRDFNEKLAALLFPQCCPPTLVSRDPTALKAFVGEHG
                     EAVLKPLDGMGGRSIFRARAGEANLNVILETLTQGSVHLAMAQRYLPEIVDGDKRILL
                     VDGEPVDYCLARIPQGDEFRGNLAAGGRGEGRPLTERDRWIAAQVGPEMKRRGMLFVG
                     LDVIGDFLTEVNVTSPTCIRELDAQFGLNIAGTLFDRIEATSAA"
     gene            3581..3982
                     /gene="pilG"
                     /locus_tag="PK399_00530"
     CDS             3581..3982
                     /gene="pilG"
                     /locus_tag="PK399_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HPW11646.1"
                     /translation="MAQQDDPGINLQGLRVMVIDDSKTIRRTAETLLAREGCDVVTAT
                     DGFEALAKIADHNPQIIFVDIMMPRLDGYQACALIKNNQTFKTVPVIMLSSKDGLFDK
                     ARGRIVGSEQYLTKPFTREELLGAIRKYVNA"
     gene            4001..4366
                     /locus_tag="PK399_00535"
     CDS             4001..4366
                     /locus_tag="PK399_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW11647.1"
                     /translation="MARILLIEDSPTEAAVMTQLLERNGHHVTTSGSAEDGIATCRRE
                     KPDLVLMDVVLPGMNGFQATRALSRDVDTSHIPVLIVSTKGLETDRVWGLRQGAKDYI
                     VKPPRESDLIARINALIGH"
     gene            4648..5199
                     /locus_tag="PK399_00540"
     CDS             4648..5199
                     /locus_tag="PK399_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPW11648.1"
                     /translation="MSSRPRDKRSPFLVLADYEQRSLAHVAGLPEQLDAPGLWRGVAY
                     RVGSHRLASGFDEVVEIMPLPLVSHVPGSLPWMLGVANVRGTLLPVVDLKQFLEGERT
                     VLHESQRVLVVRQPGGDVAVTIDELYGQRSFVDGQGIELDSVSEGRYAYFIDRAYRVN
                     DQDWGVFSLDRLARTPEFRQAAA"
     gene            5233..7248
                     /locus_tag="PK399_00545"
     CDS             5233..7248
                     /locus_tag="PK399_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPW11649.1"
                     /translation="MSTLMDSVAGKGRNISTNFWIWLLIGSLLVFAGNTLYATTKASR
                     LGGASTAASRLQLNSQRLANQGREAIEGNAQAFDAFKATKGEVDEDIKTLNDRFGDQA
                     GVSGPIRTVSNTWDPLAKRADEVMASQQAVLALAGRADSFSAKVPQLQAQLNEVVRGL
                     ASGGAGSGQVFTALQQVVTSAAMARRIAELRAGGSGAQVAADALGRDSAVFDRSMNAL
                     RNGSGDIAKVTAAGAVAPLNQAATLWAGMKKDLDGIVGDSKKLIGAQASATALANGSD
                     KLLSDSESLFAAFTSFGSLKDTSIVGGVWVSILSGLLMLFSIGGLVWAQGRAQRLRYH
                     TTKELNDRNQEAIMRLLDEMGSLAEGDLTVKATVTEDMTGAIADSINFAVEQLRTLVA
                     TINDTSVQVASSAQETQATAMHLADAAEHQAQEINTATDRINEIASSINQVSRNSADS
                     AEVARRSVQIATNGAGVVRQTIAGMDSIRDQIQETSKRIKRLGESSQEIGSIVELIND
                     ISEQTNILALNAAIQAASAGEAGRGFAVVADEVQRLAERSSGATKRIESLVQTIQADT
                     NEAVSSMEQTTSEVVAGARLAEDAGTALGEIEKVSSDLSGLIEGISVAAQEQSHAATD
                     ITQTMNTIQSITAQTSQGASQTVQSIGNLAQLASDLRRSVADFKLPA"
     gene            7332..12977
                     /locus_tag="PK399_00550"
     CDS             7332..12977
                     /locus_tag="PK399_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPW11650.1"
                     /translation="MTTALRDAIDHTALGWVKPEIDETLRQARLEIEAFVEAPADVSP
                     MRACAGYLHQVQGTLRMLELQAPAMVAGEMEQLASALLEGVPGNRDDALAALMRGVVQ
                     LPDYLERLQGGHRDIPIVLLPLINDLRTARGVDALQQDALPSLLVEATDAEVEHARGS
                     LAGRNRALLDTVSAALKEDLLRVKDALDLHLRAGGGNVADLQPQAEALDRIGETLGML
                     GLESARGVIHEQRKVVQGIADGSRPPSEDDLMEVAGALIYIEHSLDDQVTRLGTGDAP
                     GDGDDQLAQESRKVMGVLAREAAANFAEVRNAFVAFVETDWDHAVLEPVPRLLAEVAG
                     AMRILNLPQPAGYLEALRRYSHAELLGRKQVPGGRQLDTLADALAGMEYYLESLRDPH
                     GQRQDLLDKTRTSLEQLRYWPLPDDDVATLSESEAVEAAPVAGEAVVFEAPQAVEAAD
                     AAAATVSVQADAPVLNAGFDAVGDEIDDEIREIFLEEFQEEITNLDELLAPWKRTPEN
                     GELLRPIRRVFHTLKGSGRLVGAKTLGEFSWHVENMLNRVLDGSRPASPAVVAFVGQT
                     RAALPQLLAALRGEASGALDIASMEAVADRLAAGEDVVLDTLGTAPVAKVAEPAVDTA
                     PAVAADTVVAHVDPVLLEILGSEVGGHLETVDAWLAQARAGDASVNDGLLRAIHTLNG
                     AFAMTEVPSVTAFTAPAEGYVKRLLAANAPADADGIAILGEVADAVRTTTAALQSTPA
                     QVPVYAALAARVASLRETLPEVSLPFIDANAEAARKAAEEAEAAEAERIAAEHAEAER
                     LAAEEAARLEAERVENERLEAERLAAEEAEAARLAAEQAEAERIAAEEAAARAEAERL
                     EAERLEAERIAALEAEAARIAAIESEAERLAAQEAARLEAERVEAERLAAQEAARIEE
                     ERLEAERIAAAEAEEARLAAERAEAERLAAEEAARLEAERVEAERIAAEEAEAARVAA
                     EQAEAARIAEEEAARAEAERVAAEEAARLEAERVEAERIAAEQAEAARLEAERAEADR
                     QEAERIVAARQEADRQEAARLAEARAEAARVEAERRAAEIAAAAKAREQAALAALLSA
                     ATSAEDPDEALDMDGLDAELVDIFVEEGGDLLDHSDGLLALLRDAPEDREAMVGLQRD
                     LHTLKGGARMAGIMPVGELGHSMETLLEAVVAQRAELGRDGVPLLERGFDRLHGMITR
                     VAARRAVGLPVGLIDELNARAETPVAPITEAADDGLVFVPAPAPKIELKPLSAPMLQD
                     GFGDDDDIGIRAPQEQVRIRADLLDRLVNYAGEVAIYRSRLEQQLAAFRGTAQEMEQT
                     NIRLRDQLRRLDSETEAQIIARYQREGAAGNETFDPLELDRFSTLQQLSRALGESAAD
                     LQALHGSMEDLTRQYETLLLQQSRVSSDLQEGLMRTRMVPFEGLVPRLRRVIRQATGE
                     TGKQAQLKLDGAQGELDRSVLERMTAPLEHMLRNAIAHGLETPEARRKAGKGDEGTIR
                     VAVRREGSEVVLQVGDDGAGLDRAAIRTRAIERGLIKPDAILSDGDLDVLILEPGFST
                     AETVSRLAGRGVGMDVVASEVRQLGGSLDIASKPGEGTTFTLRLPQTLAVTQAVFVKI
                     GDTQYAVPIASVRGVGRISRDEINKPDAVYRYGGEDYALHDLGSLVGSTTVKAEGQLQ
                     IPLLLVRSGDLRAAVAIDQVVGNREIVVKAGGPQLASVPGIFGATIMGDGRVVVILDI
                     APLVRRFAAMPRDVAPVEQVVEARRVPLVMVVDDSITMRKVTGRVLERHNFEVATAKD
                     GIDALERMADIIPDLMLLDIEMPRMDGYELATAMKADPRMRDVPIIMITSRTGDKHRQ
                     RAMDLGVERYLGKPYQEVELMRNVFELLKLERAGDGQLQ"
     gene            12974..13975
                     /locus_tag="PK399_00555"
     CDS             12974..13975
                     /locus_tag="PK399_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HPW11651.1"
                     /translation="MNRKRVVVLARAGAARDRTEAAIVQAGAEVAALLDPATAAEDDV
                     RAASPDVLLVILDALVEQALDRFDAVLGDPALEILFDDADVAAKRDAWETARWARHLQ
                     AKLHGHDDVLPPVRDADTTAPTSLEAEPKGFQAEMDALSLQVAAMPDVPQSRPPASSS
                     APGAVVIAAGVGGPDAVRQLLGGLPPQFPRPVVLRQRIEGGQYDKLVRQMQRAAQMPV
                     ALAQAGDVLQPGTVYVLADGLDLQAAPAGLVFAPADGEPTYAALRAEDSALLLLSGAD
                     VALLDQAMAMRWAGGLVVGQSADHCFDPAASNALVARGGEALGLAQLAQKLLQRWPA"
     gene            13984..14469
                     /locus_tag="PK399_00560"
     CDS             13984..14469
                     /locus_tag="PK399_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006450740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPW11652.1"
                     /translation="MSVPMQTAPDQIIRGVLIQVANARLLLPNAAIAEVLSYADPEPI
                     ADAPDWLLGRTRWRGWQVPLVAFSRFTGIADEKGGLGSKVIVLKALGGDAKRPFFALL
                     TQGFPRLVTVTETTLSAEGDDTELLEGVLSRVRLNDDDALVPDLAVIEEQIAAALAAA
                     A"
     gene            complement(14483..15214)
                     /locus_tag="PK399_00565"
     CDS             complement(14483..15214)
                     /locus_tag="PK399_00565"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPW11653.1"
                     /translation="MRLTRSHVDLPLQAGIEVALPEDVAAHLLRVLRLQVGDACVLFN
                     GDGHDYDARITIIGKREARAEIVAARRIDNESPLRITLLQGIARGEKMDWILQKATEL
                     GIARILPASSDRSEVKLDAQRADKRLAHWRGIVVSACEQSGRATLPEVAAPQSLAQAA
                     AMREGRGFILDPFAEASLSSLRGIQLHACTLAIGPEGGWSPRDREHLVAAGYEGLKLG
                     PRVLRTETAGIAAIAALQAACGDLG"
     gene            complement(15205..16578)
                     /gene="bioA"
                     /locus_tag="PK399_00570"
     CDS             complement(15205..16578)
                     /gene="bioA"
                     /locus_tag="PK399_00570"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HPW11654.1"
                     /translation="MIAAADAWRARDLDVLWHPCTQMREHPDVLPLVPIERGEGAWLV
                     GRDGRRYLDAVSSWWTNLFGHAEPRIARAIAEQAMTLEQVILAGFSHQPAVELAEKLL
                     AIAPRESGRAALSKVFYADNGSAGVEVALKMAFHWFRNRGEDRRSKFVALENGYHGET
                     IGALSVGDIPLYRRVYAPLLAECLFAPSPDSYAAGPGETAIDCADRAADGLRDLLERH
                     PGEVCALILEPRVQCAGGMRMHDPRYLRRARELCDAHGVFLVADEIAVGFGRTGSLFA
                     CEQAGVQPDLLCLSKGLTGGFLPLAAVLCTQAIYDGFLDDSRERAFLHSHSYTGNPLA
                     CAAALASLAIFESDDILARNRATAARMSRLAAPLAALPHMADVRQAGMIVAFELTQGG
                     DKRTPFDPALRVGLRAYRAALRQGVVLRPLGDTLYWMPPYCIDDEQLQLLARVTRDAI
                     LEATACA"
     gene            16632..17180
                     /gene="nudE"
                     /locus_tag="PK399_00575"
     CDS             16632..17180
                     /gene="nudE"
                     /locus_tag="PK399_00575"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HPW11655.1"
                     /translation="MSRPLPIIHGITEHDESPYRMERLDLEFSNGERRRYERLHGRGH
                     GAVAVVPMLDAETVLLVREYAAGVHRYELGLVKGRIDAGESPIEAANRELMEEAGYGA
                     RDLQVLRSITLAPVYMSHQTHLVLARDLYPQRLPGDEPEELEVVPWKLDDLGELILRE
                     DFSEGRTIASLFIAREWLRQQR"
     gene            17177..17971
                     /gene="cysQ"
                     /locus_tag="PK399_00580"
     CDS             17177..17971
                     /gene="cysQ"
                     /locus_tag="PK399_00580"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017912917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HPW11656.1"
                     /translation="MILSNEQRDAVIALARAAAAEILAVYESPFAIEHKDDRSPLTAA
                     DLASHRCIVAGLHALTPDIPVLSEESRDLDIAARREWSTFWLVDPLDGTREFIKRNGE
                     FTVNIALVEDGVASWGVIQQPVTGALWHGGAGHGSFIREGDAGDVPIHARIPASEPLR
                     IAASRSHRDERTQAVIDALPGSDVVGCGSSLKFCRIADGGIDLYPRFGPTSEWDTAAG
                     QAILEGAGGAVLDPRGRPFRYNQRDTLLNGDFIALGDIALRDRLPA"
     gene            17968..18813
                     /gene="mazG"
                     /locus_tag="PK399_00585"
     CDS             17968..18813
                     /gene="mazG"
                     /locus_tag="PK399_00585"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPW11657.1"
                     /translation="MSEPGPVESRQVESLLAIMAKLRDPDGGCPWDLAQDFSTIAPYT
                     IEEAYEVADAIDRNDLVDLKDELGDLLLQVVFHAQMAKEQGAFEFADVVAAICDKMTR
                     RHPHVFAEEARKDDADAQLRHWEDLKRAERAAKGDADASALAGISRGLPEWQRAVKLQ
                     HKAAKVGFDWPGPEPVLEKLHEEIEEVRVEFAAVAASPDDAAARDRLEDELGDILFVC
                     ANLARHAKVDMGSALRRANLKFERRFRAMEALAAADGGLAGQSLEAQDRYWDRAKAAE
                     KGAAA"
     gene            18810..19127
                     /locus_tag="PK399_00590"
     CDS             18810..19127
                     /locus_tag="PK399_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HPW11658.1"
                     /translation="MSSDAQHEFASFSEFYPFYLGEHRNTICRRLHFTGTSFALGFIL
                     LAVTSGNAWWFLGALLSGYAFAWIGHFFFEKNRPATFKHPFYSFAGDWVMYKDILVGK
                     IPF"
     gene            complement(19180..19521)
                     /locus_tag="PK399_00595"
     CDS             complement(19180..19521)
                     /locus_tag="PK399_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPW11659.1"
                     /translation="MPYDRFLPPVLLLFSNVFMTFAWYAHLKHKDVALWKVILASWGI
                     AFFEYLLMVPANRMGSALYSAPQLKGMQEVITLLVFAGFSIWYLGQPLKWNHWAGFAL
                     IAAAAWLIFLE"
     gene            19626..20336
                     /locus_tag="PK399_00600"
     CDS             19626..20336
                     /locus_tag="PK399_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW11660.1"
                     /translation="MRAQEQAGLVLIVEDNRNISEMVGEYLESRGFEVDYASDGLDGY
                     RLAAENTYDVIVLDLMLPRLDGIEVCKRLRNEARKSTPVLMLTARDTLDEKLTGLSSG
                     ADDYLTKPFAIQELEARLRALIRRERRQVGSEVLKVADLVLDPASLRATRGGSELQLS
                     PIGLRLLTILMRESPRVVTRQEIEREIWGNSLPDSDTLRSHLYNLRKVIDKPFDRPLL
                     HTVQSAGYRIADIASQLG"
     gene            20353..21378
                     /locus_tag="PK399_00605"
     CDS             20353..21378
                     /locus_tag="PK399_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW11661.1"
                     /translation="MEKTQRHLREIFLLQLAIGCLVVVLCVLGVQAMYGFAATPWVSA
                     PVLVVALGAVIGVTWMSYRATRRVIEPMDWLLREVARWDPQRPDTAALAPANVPAAVQ
                     GDVRKMAEALHALGQRLDTYVTRERDFTRDASHELRTPLTVIRVATDLIGHDEGLSTR
                     SRRSLARIQGAAESMESLMSALLLLARDSEVPLETEDFDVREVVEHEMEKVRSLLEDK
                     GVSLLLDVQAEPVLHAPPRVLQVIVCNLLSNAARFTDAGSVQVRLLQDRIEIEDTGIG
                     MDAAALARAFEPFYRASIEQPVGPGLGLSIAQRLGHRCGWPLQLASVPGQGTRASILF
                     GARNRDQ"
     gene            21475..23025
                     /locus_tag="PK399_00610"
     CDS             21475..23025
                     /locus_tag="PK399_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW11662.1"
                     /translation="MNQPRRRPAFPLRKLGIAAIVIAVAAGGWYFWGKRGASADGGYR
                     TETVQRGDVRVAISSTGTLSAISTVTVGSQVSGQITEVLVDFNDRVTKGQVLATIDAS
                     TYQAQIEQGSAQIASAQASLRQAQAGLRNAQLDYNRKADLGRQQLVSKSDVDLARSSL
                     EQAQAQVNSAQAQIRQQTASTQTTRVNLQRTVIRSPVDGVVLTRTIEPGQTVAASLSA
                     PELFTIAEDLAKMKIELAVDESDIGQVKVGQVVSFSADAFPERQFKGVVDQVRLSATT
                     SNNVVTYPVVVTVDNSDGTLLPGLTVNAEIEVSKRSDVLKLANAALRFKPAEGSPLAA
                     LQGAGPGQGPGAQGGRGAGMSEDLQALAATLALKPEQQAAFDAALEQMKQRQAERMAQ
                     APAQGQQGGNRMFGGGPPRMGGGQGGGDASMQAQMRARMRDRFNQQFAAFRDTLDDAQ
                     RAKWDGARDAQLNAKRVTIYKLVDGKPQVAMARLGASDGSSTEISGRDIAEGDLVIAG
                     EQSATESK"
     gene            23025..23747
                     /locus_tag="PK399_00615"
     CDS             23025..23747
                     /locus_tag="PK399_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW11663.1"
                     /translation="MAVGVAEAAVPVIRTQGLSKIYSAGTEAEVAALRGVDLVIDRGE
                     FVAIMGPSGSGKSTLMNLIGCLDTPTAGKYFCDGVDVSTLDKEALATLRRDKIGFVFQ
                     GFHLLTRMDATDNVAMPLGYARIPPAQRKELARAALASVGLAERAGHRPNELSGGQQQ
                     RVAIARALVNKPPILLADEPTGALDSKTGEEILALFKRLRDEGHTVILITHDAHVAAH
                     ADRTYVMRDGELHAQTEAEAAA"
     gene            23744..24979
                     /locus_tag="PK399_00620"
     CDS             23744..24979
                     /locus_tag="PK399_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW11664.1"
                     /translation="MTLLDILRTAFFALRGNWMRSALTSLGVIIGIAAVIVMVSIGQG
                     TQAEIDKMVSGLGSQRLDIGGAGGMGPGGARMAQGSRWSLTGGDVEAIRNEVPTAQYA
                     SGSLRGNTQVVFAENNASTSWQGVEADWFAINDWKVARGDGFSANDYGGSAKPVILGE
                     TVRKTLFGEEDPIGQTVRLGRVPFTVVGVLGAKGQGGFGQDQDDMVVVPLETARRRLS
                     NSQSMPPGAVQAISVGVDDAKNLASAQAEIEALLRQRHKIEPGAEDDFAVRNISQIVA
                     TRTATTNLMSKLLGAVAGICLVIGGIGIMNIMLVSVTERIREIGLRMAVGAGPGDVQR
                     QFLAEAMLISLIGGVIGIAIGVVGALVVGKLGDLPVELNAKVVMLAAAFSVGTGLFFG
                     YYPARKASQLDPIEALRSQ"
     gene            25118..25498
                     /locus_tag="PK399_00625"
     CDS             25118..25498
                     /locus_tag="PK399_00625"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HPW11665.1"
                     /translation="MADIYGHLPRGPAGIMRAAVWSLQGLRAAWLHESSFRLEVCLFV
                     VLGPLGWFLGATGVERALLIGSCLLVMAMELMNSSMEAVIERYGAERHELAGRAKDMG
                     SAAVFVLMMNVLLVWALILGPRYL"
     gene            25533..26282
                     /locus_tag="PK399_00630"
     CDS             25533..26282
                     /locus_tag="PK399_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPW11666.1"
                     /translation="MDLSFLASPDAWLTLVTLSALEIVLGIDNLVFISIAVSRLPEAQ
                     RPKARKIGIAVACVTRIMLLVMLAFLATMEQDLFVLFGMGISIRDMVLIVGGAFLLVK
                     GTLEIRDLISGGEDEDPRTTKTSSVFWMVIAQIAVIDIVFSLDSVITAVGIAQHIPIM
                     VFAILLAVAIMLLAANPLGKFIDDNPTVKMLALAFILLVGAVLILDGLGIHVPKPYIY
                     FAMAFSVLVEWLNLLMRRKAAANYVPGAGEW"
     gene            26360..27079
                     /locus_tag="PK399_00635"
     CDS             26360..27079
                     /locus_tag="PK399_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPW11667.1"
                     /translation="MRGLSLVRGALLALVVVMLSGCGYNQIQSKDEAAKAGWSEVLNQ
                     YKRRADLIPNLVATVKGYAAHEAQVLTAVTEARAKVSQVNVNADDAASLAQYQAAQGE
                     LSQALGRLMMVTEAYPNLKADQQFIGLQTQLEGTENRVTVARGRYIQLVQDYNTYIRQ
                     FPVNLTAMIFGYKPKPNFTVENEAQIQDAPAVDFATPPPAPVAPPAPAVPPGIVNPPQ
                     PAAPPVQGGNAPQDPQDAPAA"
     gene            27111..28022
                     /locus_tag="PK399_00640"
     CDS             27111..28022
                     /locus_tag="PK399_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HPW11668.1"
                     /translation="MPSSQRRAFAALRAVLWCACLLLSLVLPAWAQAPIPTFASPVVD
                     TTNTLDAATRQKLEQQALALQQRKGSQLQVLMIPSTQPEGIEAYAVRAFESFKLGRKG
                     IDDGVLLVIAKDDRKARIEVGYGLEGAIPDIAAGRIIQEYMVPKFRQGDYAGGVEDAT
                     AQLVKLVDGEPLPEPVASNSTGRDSGDEWLFALFAAFMASLVARSMFGRLPVGLRILF
                     TVIAGGVAAWLFSWVVFVGGVGAVIGFFTALMGGGGGGGGRYARHHDWGGFGGGSGGG
                     GWGGGSSGGGWSGGGGMSGGGGASGGW"
     gene            28036..28539
                     /locus_tag="PK399_00645"
     CDS             28036..28539
                     /locus_tag="PK399_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPW11669.1"
                     /translation="MAGTLKRLFTHLFTADARGLFPTSAMERIAAAIDVGETCHAGEV
                     CFAVESSLHWRDVLRGVQARARAEDAFARLRVWDTAANNGVLVYLLLADHRIEIVADR
                     GLAPFVSAEQWRRVCELMEGRLRAGEHADGVVAGIEAVGDLLAEHFPQEPGAPDEDEL
                     PDQPVFL"
     gene            28682..29605
                     /gene="lgt"
                     /locus_tag="PK399_00650"
     CDS             28682..29605
                     /gene="lgt"
                     /locus_tag="PK399_00650"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPW11670.1"
                     /translation="MPYLHQIDPIALDLGTWNLPLLGSVHPQVHWYGVMYLLGFIVAW
                     WLGRRRIRAGRLPGVDENGFGDLMFYAMLGVVLGGRIGYILFYDFASFAANPAMVFKV
                     WEGGMSFHGGLLGVLVAGAWWSRRQRLHFFDTVDFVAPLVPAGLGFGRVGNWIGGELW
                     GKPTQGTAFDGWGVVFPRALPDPFASMDAATLKQQFDAGILDSFARHPSQLYQATLEG
                     LVMFAVLAWYSRTPRPRYAVSGLFALLYGCFRFLVEFVREPDQQLGYLAFGWVTMGQV
                     LSLPLILLGLFLCWKSHSSPTLQPQPADTAK"
     gene            29617..30411
                     /locus_tag="PK399_00655"
     CDS             29617..30411
                     /locus_tag="PK399_00655"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPW11671.1"
                     /translation="MRQYLDLLRHVLEHGTEKADRTGTGTRSVFGWQMRFDLNDGFPL
                     VTTKKLHLRSIIHELLWFLQGQTNIAYLKQHDVGIWDEWADADGELGPVYGKQWRSWE
                     GADGEAVDQIAWLIDEIKRNPDSRRLIVSAWNVADLPKMALMPCHSLFQFYVADGKLS
                     CQLYQRSGDIFLGVPFNIASYALLTHMVAQVCGLGVGDFVHTLGDAHLYSNHVEQARK
                     QLSRSPRALPTLALNPEVRDIFAFTYEDIAIHGYDPLPAIKAPVAV"
     gene            30430..30933
                     /locus_tag="PK399_00660"
     CDS             30430..30933
                     /locus_tag="PK399_00660"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPW11672.1"
                     /translation="MHVSLIAALDRDFAIGKGNALPWHLPDDLKRFKALTLGKPILMG
                     RKTAESLGRALPKRRNLVLTRKGRVPFDGMQAVSSIDDAMAIARHDGDELCIIGGGEV
                     YVLALPHATRLHLTHVDTRVEGADAFFPRFDAAAWREVGRTVHPADAMHAFAFDFVDY
                     VRAVGTD"
     gene            31080..32300
                     /locus_tag="PK399_00665"
     CDS             31080..32300
                     /locus_tag="PK399_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW11673.1"
                     /translation="MAKPAVATTPGTDAQEAATPPAIAPNLLSFANGTIMPLPTDALL
                     VTAPILMIDGSSIQNWVGDGKPQSFVFELPEAATLRMLEFDNDTSGMGGVDAGIKDLT
                     VEVSTTSATEGFQQVFAGSLAKGVNGQRFDIAKPVPGRWVRANFASNHGGESYSLAEL
                     RAFGDAPPAVLSTDASGSYETVWGTWNITQAGTTISGCFQPGGLSTQPATFSGGMEGN
                     IARIRYIETDDAGAPREARALLLVFARDGKRFFTAGVDGESLSDYGEVKRIAAEPATC
                     PGRAANAPARTMADTLAKEGRVAVYGINFDFDAATLRPESTVVLEQVAGLLRDDPALR
                     ITIEGHTDDVGGAAYNDALSGKRANSVKDWLVAAGIDGARLEAVGKGAGSPVATNGND
                     VGRAQNRRVELARR"
     gene            complement(32327..33238)
                     /locus_tag="PK399_00670"
     CDS             complement(32327..33238)
                     /locus_tag="PK399_00670"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HPW11674.1"
                     /translation="MATWAIGDLQGCYDVTQRLLEKIRFDPAVDRIWFCGDLVNRGGQ
                     SIETLRLVHSLREVSHVVLGNHDLSLLAIGERSPDEQRKVNPDLQGVLFAEDARELLD
                     WLRMRELAHVDRDLGWMMVHAGLAPKWTTQLAEKHAREVESRLHGDNYRRLLKNMYGD
                     GPDWSPGLGGAERDRAIINIFTRMRYCSPRGRIAFEHKGEPGTQPPGLYPWYSVPGRA
                     NRDLKVVCGHWSTLGLFIGQGVHALDTGAVWGGKLTALQLDAETLHVVQVPGRDVPAT
                     PPRQRPPRPKPGNRPHKPPGAQRRDQA"
     gene            complement(33248..33628)
                     /gene="apaG"
                     /locus_tag="PK399_00675"
     CDS             complement(33248..33628)
                     /gene="apaG"
                     /locus_tag="PK399_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HPW11675.1"
                     /translation="MSNTDYQFDIEVATRFLDEQSVPEEGRFVFAYTVHIRNDGKVPA
                     QVLGRRWLITDGNGKVQEVVGEGVVGEQPWLRPGEGFEYTSGAVLETDIGTMRGSYDV
                     LADDGTRFAAPIPPFTLSIPRTLH"
     gene            complement(33675..34481)
                     /gene="rsmA"
                     /locus_tag="PK399_00680"
     CDS             complement(33675..34481)
                     /gene="rsmA"
                     /locus_tag="PK399_00680"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPW11676.1"
                     /translation="MTASFTEPAKKHLGQNFLHDRGVIDKIVQAIDPKPGDAVVEIGP
                     GQGALTFPLLKRHGAITAIEFDRDLHAPLQAAARDHGALHLIEGDVLGVDFSALAAEL
                     APAGGKIRLAGNLPYNLSSPILFHALDHAQAIRDMHFMLQKEVVERMAAGPGSKVYGR
                     LSVMLQAYCNVTPLFVVPPGAFRPAPKVDSAVVRLVPKPAESILVADRRVFANVVRAA
                     FGQRRKTLRNALNGVADVAAIEAAGLRADARAEQVEVAGFVRLANLLAQG"
     gene            complement(34478..35452)
                     /gene="pdxA"
                     /locus_tag="PK399_00685"
     CDS             complement(34478..35452)
                     /gene="pdxA"
                     /locus_tag="PK399_00685"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPW11677.1"
                     /translation="MRPRLALVPGEPAGIGPELIVRAAQQDWEAQLVVHGDAATLHRA
                     GDALGLPIALHRNGESPRSPRSLELVEIAHPAAAAFGQPDPANAESVVASLRRAAQAC
                     LTGGCHGLVTGPVHKGVINQGGIPFTGTTGLLATLAGCEVVMMLANPGMRVALQTVHL
                     PLREVADAITADALGSTLRIVDQALRRQFGIASPTIAVLGLNPHAGEDGHLGREELDI
                     IDPALERLRGEGLSLVGPLPADTAFLPGKLRGFDAVLAMYHDQGLPVLKHADFANAVN
                     ITLGLPYPRVAVDHGTALDLAGKGMADPSSLFAAIHTCTQFARHRMHA"
     gene            complement(35465..36829)
                     /locus_tag="PK399_00690"
     CDS             complement(35465..36829)
                     /locus_tag="PK399_00690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW11678.1"
                     /translation="MTPAFSKRASRPLLAAALASLLLVGNVQAQSYAGSQPVDRIAAV
                     VNEDVILRSELDRAVANIRSQYAGKEAQLPPADVLERQVLERLVLMRLQIARATDSGI
                     NASDEELERAVQGVAQQNSMSVDQLRQRLAQDGMAFTEFRNNLRDEIITQKLRQSFAQ
                     SRINVSEAEVDAAMATASTAASQQFHLAHILVGAPDGATPEQLAVAQKKIEGVQALIA
                     KGEMTFAAAAVRYSDSPNALEGGDLGWRGLNEIPPAFAQTIQQMQNGQVIGPIRGPSG
                     FQLLQLVETRTQAGGSGEQVTQFSARQIVVKVDDKTDDAAAKAKAETLAARLAGGAEF
                     AKLAGESSDDAATKRRGGDMGWFGADTYGTDFGMQVASLSDGQTSAPFKTEAGWVIVQ
                     RIGSRQVAAADESQRAQLRETIGRRKLEEEWNRFLREMRGEAFVDVRADKAETAATAP
                     ASGG"
     gene            complement(36826..39123)
                     /gene="lptD"
                     /locus_tag="PK399_00695"
     CDS             complement(36826..39123)
                     /gene="lptD"
                     /locus_tag="PK399_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010481829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HPW11679.1"
                     /translation="MRPVLRLLPLPLCIAFSLHAHAADDPPNWGLCPVQDVVKPFVEA
                     QAAPEGINIDNSSQSTDIEGDALSGTEANPVFQGNVTMRRGDQFMGADQITFDKEADR
                     YTAEGSIRYQGSGLRMVAARAEGNQGTETHVIHDLDYQLLSRRGNGGADSVTLAGEMG
                     TLRGATYSTCPPDARQWELRARQIDIDTEEGMAVARGATLRVGKVPVLYMPWFMFPTD
                     ERRRTGLLFPSISNSSRNGFDYRQPIYLNLAPNYDATLNPRLMTERGVQLGAQFRYLV
                     EGGQGVVEGTWMPDDKLRDRDRSLLRYDAFQNISSHWQARANLNWISDSRYYEDFSNS
                     LDGLSQTSAYSAIGVYGRGQGWDAGVTADHWQLADFTLTEEALPFSRLPRAYLNWEQR
                     LAGLLLGGIGAEAVRFEHEAYDAGSRIDLKPWISLPLEGNSWFLRPTAAYRYTGYSLD
                     RGLAQALGGSSPSRGQSIFSLDAGLFFDRDTTVKGKEYLQTIEPRLFYLNVPYADQTD
                     MPLFDTQPLTFSWGQLFRDNRYSGADRQADANQLTLAVSTRMIRQSDGRERFSASLGQ
                     IRYFNESRVFIPGEPITEKGQSAWVADANYAPTDRWTIGASYQWDPKFKRTDLASVRA
                     RYLLPDEGVFNLGYRYRRSLLEQVDVAFLYPVSASWSVVGRYYYSLHDSKTLETMAGL
                     QWDSCCVAVRLVGRRYVHNRQGDLSNALMFEIELKGLGSAGQDTRRALRRSILGYNRD
                     DLYLVPPQTATGQPTPPDSAADTLP"
     gene            39258..39662
                     /gene="msrB"
                     /locus_tag="PK399_00700"
     CDS             39258..39662
                     /gene="msrB"
                     /locus_tag="PK399_00700"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPW11680.1"
                     /translation="MNDHHLPTTDAEWRERLDPQQYAVCRCSATEPPFTGKYWDHKGS
                     GVYACAACAAPLFASADKYDSGSGWPSYLRPVSAEAVTEHEDGTHGMRRTEVRCARCD
                     SHLGHVFPDGPAPTGLRYCINSAALAFVPDPA"
     gene            complement(39676..40587)
                     /locus_tag="PK399_00705"
     CDS             complement(39676..40587)
                     /locus_tag="PK399_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016900762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HPW11681.1"
                     /translation="MRVYTHPACLAHDTGPDHAERPARLTAVVDALRRELPALEWHEA
                     PRATRGQLLRAHAPDLLDAVLETHPAQRILLDPDTVLSPTSAEAALRAAGAAVAAVDA
                     VLSGKTRRAFCAVRPPGHHATCDVAMGFCLFNNIAIAALHALDKHGLERVAIVDFDVH
                     HGNGTQAIFERDPRVLFASSHQWPLYPDTGARNETGVGNIVNMPLPAEAEGVLFRRAW
                     DDVLLPAVDDFRPQLLLVSAGFDAHWRDPLAQLRLQAGDYAWITDALVALANRHCGGR
                     IVSSLEGGYDLAALAESSVAHVGALCA"
     gene            complement(40613..41164)
                     /locus_tag="PK399_00710"
     CDS             complement(40613..41164)
                     /locus_tag="PK399_00710"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPW11682.1"
                     /translation="MGNRLSKIYTKTGDDGTTGLGDGSRVAKDSARVIAYGTVDEANS
                     CIGLLLAATLPDDVREVLVRIQHQLFDLGGELCIPGHAAIFDADVEALEARLDHYNDD
                     LPALKDFILPGGGEAAARCHIARTVVRRAERDSVTLSRHDAVRPQAIRYLNRLSDLLF
                     VLARVLARADGQGEVLWEHARRS"
     gene            complement(41183..41752)
                     /locus_tag="PK399_00715"
     CDS             complement(41183..41752)
                     /locus_tag="PK399_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11683.1"
                     /translation="MNRHAPTPAAGPLMLAALIFIAALTRVIPHPPNFSPIEAVALFG
                     GAYFAKRHWALLVPLLAMFASDLVLGLLNGGIYWDYFTSAGYLLVYACIALSTLLGFG
                     LRGKVGAGRVLGYSLAGSMLFFVVTNFGAWLSDPLYPKSMAGLLACYAAGVPFFQWTV
                     LGTLFYSALLFGGFELMRQRLPALRPQTV"
     gene            41797..43029
                     /gene="ubiH"
                     /locus_tag="PK399_00720"
                     /gene_synonym="visB"
     CDS             41797..43029
                     /gene="ubiH"
                     /locus_tag="PK399_00720"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HPW11684.1"
                     /translation="MPNTDRDATTPHDVLIIGGGLVGASLAIALDGLGLDVGMVEATQ
                     AGALPAVFDQRNLSFAEATVNALDALGVMRLLRAPSGSIRRIHASRAGDFGRIRLEAS
                     AHGREEFGRVVVARDFGEALEARLASLQHLTRYRPARFIGLGDTRDGLRHVRISDADG
                     EREIATRLLVAADGTRSGVREALGIRVREHDYRQTLLVARLRTQRPPDGTAYERLTSS
                     GPTALLPRGDGHYGLVHGVASDDAEAVAAMDDAVFLARVQVAFGWRAGRFLSVGPRSA
                     YPIIGCVADALVATRAVLVGNAAQSLHPLGAQGFNLGLRDALTLAECIAAQPGDAGDD
                     PLLRHYAERRADDRTRTLAFSDGLAKLTSNDAPLLRPLRSAGLLAGDAMGWLQGWLVG
                     GAMGYRGDVPALCRRGVA"
     gene            43026..>43974
                     /locus_tag="PK399_00725"
     CDS             43026..>43974
                     /locus_tag="PK399_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW11685.1"
                     /translation="MSRRGPLDVVVAGGGVVGAATALMLAREGLQVALVEPRQPPAWQ
                     RTIRDLRVYAFAPDNAALFAELGAWPGVLDSRVQPYRRMQVWDAAGGDALAFDADALG
                     QPQLGWIIENNALVDALWRLLPTAGVRLHCPASIEALEQDGTRVRVGLDDGLVLDARL
                     AIAADGGQSALRTLAGIGTTSHDYRQQGVVAFVASEQPHRDTCWQRFLPTGPVALLPF
                     NDDGDAGLRGRLGSIVWTLPDVEARCLRDADPAEFELALATAFGGELGAFHLQSARAA
                     FPLRRQLADTHVAGRVLLLGDAAHVVHPLAGQGVNLGLRD"
BASE COUNT         6919 a        14751 c        15476 g         6828 t
ORIGIN      
        1 gctgccgttc gccgcgcccg acgatccggt gttcgaggcg cgcctggatg cgatccgccg
       61 cgccggcggc aatccgttcc gcgacgaaca actgccgcag gcggtgatcg cgctcaagca
      121 gggcgcgggt cgcctgatcc gcaccgagac cgaccgcggc gtgctggtgc tgtgcgatcc
      181 gcgcctgacc cagaaaagct acggcaaggt cttcatggat tcgttgccgc cactgccgat
      241 cacccgcgag gtcggcgacg tgcaggcgtt cttccggtca caatccaccc catgagactg
      301 ctcgccttcg aaaccgccac cgaagcctgt tccgtcgccg tgtacgtcga cggcgagatt
      361 tgcgaacgtt tcgacctggc cccgcgccgg catgcggaac tcgccttgcc gtgggcgggc
      421 gccatgctgg ccgaggccgg catcgccagg tcgcagctcg atgcgatcgc ggtcggtcgc
      481 ggtcccggtg cgttcaccgg cgtgcgcctg gcgatcgcca tcgcccaggg catcgcgttg
      541 gccttggacc ggccggtgct gccggtatcc acgcttgcgg cgctcgcgat gcgtgctggc
      601 gcaagcaacg aaggcgatcg catccttgcc gcgatcgacg cgcgcatggg cgaggtctac
      661 ctgggcgcgt tcgcccggcg tggcgatgac ctcgtcgccc tcgacaccga agtcgtggtc
      721 aagcccgagg gtgccagcat cccggatggc gatggctggc atggcgtcgg caccggtttt
      781 gccgcagcag ggaacgcctt gccgctccgc ctggaagggc gactggccgc ggtcgatgcc
      841 accgcactgc cgcacgctgc cgacgtggcc cgccttgccg cgctggcgta cgcgcgtggt
      901 gaagcagttg cggcggaacg catcgagccc gcctacctgc gcaacaacgt cgccctgacc
      961 cttgccgagc agcaggcctt gcgcgcgaaa ggttgacgag cgtcgcttgt gggcctcggc
     1021 agacgggcta tgctgcaaag cagcagtcca gcggcgaggt ggtacatggg atgcaggaat
     1081 tgggtgttgg ccgggatgct ggtggcgatg ccagcgctgg cagaagacgt tgtgttcgaa
     1141 ggccatgccg tggaggtcga cgccaccagc gaggcgcgtc cgatcctggg catgcgcggg
     1201 acgcgctact cgatcccggg atcggtgtcg cagatcgtcg gcaaggcgga acaatgcgcg
     1261 acccgccagc ctggcgcact caccgtcgag tcggtggagg ccgatgccgg gcggctggtg
     1321 gccggcggcc gtgccgagta ccggcagaaa ggccggcgca gcgtacgtgc acggatgacg
     1381 ctcgaggcgg gtgaaggcac tttccgggtc gtcttcaacg acctggcgac ggcatcggcc
     1441 gatgccggtg gtttcgcgcc gctgatccag caggacgccg caggctggga aaacgccctg
     1501 gaagcgctca tcgccatcga gcaaccgctg ctcgactgca tgttccgctg agcagccctt
     1561 ctccggcccc tagcggtcga tcagctcgcc acccggcagg aactgccagg tgcgggtgac
     1621 gtgcagcacg tcggggtctt cctgggtctt cggcagcggc gagaacggct gcgacagctt
     1681 gacgatgcgg gtcgcggcct cgtccagcat cggcacgtgg ctggactgga cgatgcggat
     1741 gctttccacg ctgccgtcgc ggcgcacgcc cacgctgatc acgaccagtc cgccgatacg
     1801 gcggcggcgg gcttcgtccg gatagttgag gttgccgact cgctccaccc ggtccaccca
     1861 gccgcgcagg tagtcggccc acgcgtattc cttggtgctg gcggagacga acttgcgttt
     1921 cgggcgcttg gcgtattcct gcgagcgcaa ctggatctcg gcggcgagcc gcgccatctc
     1981 gatgtcgcgg tcgatcttct cgcggcctcg cggcgcctgg ttggcatcca cctgctgcag
     2041 aggctgcggc atcggcaacg cctgctggcc attggccgat gccacgaccc gcgcatccgg
     2101 cggcggcgtg ggcgcgggcg attgcgcatg cagcgggcgc ggcgccaggc cgtcctccgg
     2161 ctgcggcagt ggcccggcct gcggggcggt ggggcggctg ctcttgtcgt gctcgccacc
     2221 accctggttg ttggcctggg ccaggaaatc cgcctgcgcc ggggtcaggg gtgtctgcgt
     2281 gcggctcagg atcacgtcca gcgtgggcag catcggggcc gcatcctcca ccgcgaagcc
     2341 aatgcccagg atcaccatcg cgtgcaccag caccgacagc accagggtcg cgcccagccg
     2401 cgtggattca ccgatcacgg gcggcgcggg cagcgacgcg ggggcggcgg ccgcgcccat
     2461 caggccgccg acgtggcttc gatgcggtcg aacagcgtgc cggcgatgtt gaggccgaac
     2521 tgcgcgtcca gttcgcggat gcaggtcggg ctggtgacgt tgacctcggt caggaagtcg
     2581 ccgatcacgt ccaggccgac gaacagcatg ccgcgccgct tcatctccgg gcccacctgg
     2641 gcggcgatcc agcggtcgcg ctccgtcagc ggccggcctt cgccgcgccc gccggcggcc
     2701 aggttgccgc ggaattcgtc accctgcggg attcgtgcca ggcagtaatc caccggctcg
     2761 ccatcgacca gcaggatgcg cttgtcgcca tccacgatct ccggcaggta acgctgcgcc
     2821 atcgccaggt gcacgctgcc ttgggtcagg gtttccagga tcacgttgag gttcgcctcg
     2881 ccggcccggg cgcggaagat cgagcgcccg cccatgccgt cgagcggctt cagcaccgcc
     2941 tcgccgtgtt cgccgacgaa cgccttgagc gcggttgggt cacggctgac cagggtcggc
     3001 gggcagcatt gggggaacag cagcgcggcc agtttctcgt tgaagtcgcg caatccctgc
     3061 gggtcgttga ccacccgcgc gccttggcgc tgggccatgc cgagcaactg ggtgtcgtgc
     3121 aggtagtcgg cgtcgaccgg cggatccttg cgcatcagca ccacctggcc gggtccgaag
     3181 tccagttccc gccagtcgcc aaggcggaaa tggcggccct gcacgtcctg caccgtcagc
     3241 gcggcggcgg tcgcgaccgc gcgcgcgccg cgcagcgcca gcccgccggg gcgcacgtaa
     3301 tgcagccgat ggccgcgccg ctgggcctcc agcagcatcg cgaaactgga atccttggtg
     3361 gtcttgatgg actcgatcgg gtccatcacc accacgacgt cgagtggtgc ggcgtctgtc
     3421 atctgcgggt ccatggcggg ctgcgccgat ggtagcaggc gacgcgcagc gtccatcgat
     3481 gtgacgcatt gcggcgcttg acaggccggg tgcaggctgg gatatagaca gtccggtcgc
     3541 agtgcaaaac cgcgacgggc aatggcggca aggggaacgc atggcgcagc aagacgatcc
     3601 aggcatcaac ctccagggct tgagggtgat ggtcatcgac gactcgaaga cgatccggcg
     3661 cacggcggaa acgctgcttg cgcgggaagg ctgcgacgtg gtcaccgcaa ccgacggatt
     3721 cgaggcgctg gcgaagatcg ccgaccacaa tccgcagatc attttcgtgg acatcatgat
     3781 gccgcggctc gacggctatc aggcctgcgc gctgatcaag aacaaccaga cgttcaaaac
     3841 tgtgcccgtg atcatgcttt cgtcgaagga tggtttgttc gacaaggcgc gtggtcgtat
     3901 cgtgggctcc gagcaatacc tgaccaagcc cttcacccgt gaagaactgc ttggcgcgat
     3961 tcggaagtac gtcaacgcct gaccgggggg tagggcatca atggcacgca tactgctgat
     4021 cgaggattcg ccgaccgagg ccgcggtgat gacccagttg ctggaacgca acgggcacca
     4081 cgtgaccacc tcgggtagcg cggaggatgg catcgccacc tgccgtcgcg aaaagccgga
     4141 cctcgtgctg atggacgtgg tgctgcccgg catgaacggc ttccaggcca cccgcgcgct
     4201 gtcgcgcgat gtcgatacca gtcacatccc ggtgctcatc gtcagcacca agggcctgga
     4261 gaccgaccgc gtctggggcc tgcgccaggg cgccaaggac tacatcgtca agccgccgcg
     4321 cgagagcgat ctcatcgcgc ggatcaacgc actgatcggg cactgagcat ggccaagcgc
     4381 ggcaagcaga agcagaaggg caagccgcag gcggccgccg ccgtcgaggc cgtgtccgcc
     4441 gcgccggacg acgttgccgc catcgagtcc gtcgaggcgc cggtcgtcga gccggacgcg
     4501 gtggcgcagg tcgaggacca ggccgggccc gtcgacgcgt tcgacgccat cgccgccgtc
     4561 gaagcggtgg aggaggctgc gcccgaagtc gtcgagccgc ccgtgcagga agcgccgcgc
     4621 cctgcgcgcg ccaagccctc gcgccgggtg tcgtcgcgtc cgcgcgacaa gcgttcgccg
     4681 ttcctggtgc tggccgatta cgaacagcgc agcttggcgc acgtggccgg cctcccggaa
     4741 cagctcgatg cgccgggcct gtggcgcggc gttgcctacc gggtcggctc gcaccgtctc
     4801 gcgtcgggtt tcgacgaggt ggtggaaatc atgccgctgc cgctggtcag ccatgtcccc
     4861 ggttcgttgc cgtggatgct tggcgtggcc aacgtgcgcg gcaccctgtt gccggtggtc
     4921 gacctcaagc agttcctcga gggcgaacgc accgtgctgc acgaaagcca gcgcgtgctg
     4981 gtggtccgcc agcccggcgg cgatgtcgcc gtcaccatcg acgagcttta cgggcagcgc
     5041 agcttcgtcg acggccaggg catcgaactc gactcggtgt ccgaaggccg ctatgcctat
     5101 ttcatcgatc gtgcctaccg cgtcaatgac caggactggg gcgtgttcag cctcgatcgc
     5161 ctggcccgta ctcccgaatt ccgccaagcc gccgcgtaac ccgcggaccc atacgcaacc
     5221 cgaggtcgca acatgagcac tctgatggat tccgtcgccg gcaagggccg caacatcagc
     5281 accaacttct ggatctggtt gctgatcggt tcgctgctgg tcttcgccgg caacaccctg
     5341 tacgcgacca ccaaggcttc gcgcctcggt ggcgccagta ccgcggcctc gcgcctgcag
     5401 ctgaactcgc agcgactggc caaccagggc cgcgaagcga tcgaaggcaa tgcgcaggcg
     5461 ttcgatgcgt tcaaggcgac caagggtgaa gtcgacgagg acatcaagac cctcaacgac
     5521 cgcttcggcg accaggccgg cgtgtccggt ccgatccgca cggtgagcaa tacctgggat
     5581 ccgctggcca agcgcgccga cgaagtgatg gcctcgcagc aggcggtgct ggcgctggcc
     5641 ggtcgcgccg acagcttcag cgccaaggtg ccgcagctgc aggcccagtt gaacgaggtg
     5701 gtgcgtggct tggcatccgg cggcgccggt tccggccagg tgttcaccgc gctgcaacag
     5761 gtggtgactt ccgccgccat ggcccgccgc atcgccgaat tgcgcgccgg cggcagcggc
     5821 gcgcaggtcg cggcggacgc cctcggccgc gactccgcgg tgtttgatcg gtcgatgaac
     5881 gcactgcgca atggcagcgg cgacatcgcc aaggtgaccg ctgctggcgc ggtcgccccg
     5941 ctcaaccagg ccgccacgct gtgggccggc atgaagaagg acctcgacgg catcgtcggt
     6001 gactcgaaga agctgatcgg cgcgcaggcc tcggccacgg cgcttgccaa cggctccgac
     6061 aagctgctct cggacagcga aagcctgttc gcggcgttca cctcgttcgg ctcgctgaag
     6121 gacaccagca tcgtcggcgg cgtctgggtc agcatcctgt ccggcctgct gatgctgttc
     6181 tcgatcggcg gcctggtctg ggcgcagggt cgcgcgcagc gcctgcgcta ccacaccacc
     6241 aaggaactga acgaccgcaa ccaggaggcg atcatgcgcc tgctggacga aatgggttcg
     6301 ctcgcggaag gcgacctgac cgtgaaggcg accgtgaccg aggacatgac cggcgcgatc
     6361 gcggactcga tcaacttcgc cgtggaacag ctgcgtaccc tggtggcgac gatcaacgac
     6421 acctcggtgc aggtggcctc cagcgcgcag gaaacgcagg cgaccgccat gcacctcgcc
     6481 gacgccgccg agcaccaggc gcaggaaatc aataccgcca ccgaccgcat caacgaaatc
     6541 gcgtcgagca ttaaccaggt ctcgcgcaac tccgccgact cggccgaggt ggcgcgtcgc
     6601 tcggtgcaga tcgcgaccaa cggtgcaggc gtggtgcggc agacgatcgc cggcatggac
     6661 agcattcgcg accagatcca ggaaacctcg aagcgcatca agcgcctggg cgagagctcg
     6721 caggaaatcg gctcgatcgt tgaactgatc aacgacattt ccgaacagac caacatcctg
     6781 gcgctgaacg ccgcgatcca ggcggcctcg gctggtgaag cgggccgcgg gttcgcggtg
     6841 gtggccgacg aagtgcaacg cctcgcggaa cgctcttccg gcgcgaccaa gcgcatcgaa
     6901 tcgctggtcc agaccattca ggccgatacc aacgaggccg tcagctcgat ggagcagacg
     6961 acctcggaag tggtcgccgg cgcccgactg gccgaggacg cgggtaccgc gctgggcgag
     7021 atcgaaaagg tgtcgtccga cttgtccggg ctgattgaag gcatctcggt ggcagcgcag
     7081 gaacagtccc acgccgcgac cgacatcacc cagacgatga acacgatcca gtcgatcacc
     7141 gcgcagacgt cgcaaggcgc cagccagacc gtgcaatcga ttggtaacct cgcgcagctc
     7201 gcgtccgacc tgcgtcgttc ggtcgccgac ttcaagctgc cggcctgagt gcaggcaggg
     7261 acaccagggg tcgggatgaa cagcacccac caggcaagcc ccgcgttgcg gggcgatagc
     7321 ggagcttcgc gatgaccacg gcgctgcgcg atgccatcga ccataccgcg ctcggctggg
     7381 tgaagcccga gatcgacgag accctgcgcc aggcgcggct cgagatcgag gccttcgtcg
     7441 aagcccccgc ggacgtgtcg cccatgcgcg cgtgcgccgg ttacctgcac caggtgcagg
     7501 gcaccctgcg catgctcgaa ctgcaggcgc cggcgatggt ggctggcgag atggaacaac
     7561 ttgccagcgc gctgctggag ggcgtgccgg gcaaccgcga tgacgcgctg gcggcgttga
     7621 tgcgcggcgt ggtgcagttg ccggattacc tggaacgcct gcagggcggc caccgcgaca
     7681 tcccgatcgt gctgctcccg ctgatcaacg acctgcgcac cgcgcggggc gtggacgcgc
     7741 tgcaacagga cgctttgccc agcctcctgg tcgaagcgac cgatgcggaa gtggaacatg
     7801 cgcgcggcag cctcgccggg cgcaaccgcg ccttgctgga cacggtgtcc gcggcgctca
     7861 aggaagacct gctgcgggtc aaggacgcac tcgacctgca cctgcgcgcg ggtggcggca
     7921 acgtcgccga cctgcagccg caggcggaag cgctggatcg catcggcgaa accctgggca
     7981 tgctcgggct ggaaagcgcg cgtggcgtga tccacgagca acgcaaggtg gtgcagggca
     8041 tcgccgacgg tagccggccg cccagcgaag acgacctgat ggaagtcgcc ggcgcgctga
     8101 tctacatcga acattcgctg gacgaccagg tcacccgcct cggcacgggc gatgccccgg
     8161 gcgacggcga cgaccagctg gcgcaggaat cgcgcaaggt gatgggcgtg ctggcgcgcg
     8221 aggccgcggc caacttcgcc gaagtccgca acgccttcgt cgctttcgtc gagaccgact
     8281 gggaccacgc ggtgctggag ccggtgccgc gcctgctggc cgaagtggcc ggcgcgatgc
     8341 gcatcctcaa cctgccgcaa ccggcgggtt acctggaagc gctgcgccgc tacagccacg
     8401 cggaattgct gggccgcaag caggtgcccg gcggacgcca gctggacacc ctggccgatg
     8461 cactggccgg catggagtac tacctcgaat cgttgcgcga tccgcacggc cagcgccagg
     8521 acctgctcga caagacccgc accagcctgg aacagctgcg ctactggccg ctgccggacg
     8581 acgacgtggc caccctgtcc gaatctgaag cggtcgaggc tgcgccggtc gccggcgagg
     8641 cggtggtgtt cgaggcgccg caggccgtcg aggctgccga tgccgcggcc gccacggtca
     8701 gcgtgcaggc cgatgcgccc gtgctcaatg cgggcttcga tgcggtcggc gacgaaatcg
     8761 acgacgagat ccgcgagatc ttcctggagg agttccagga ggaaatcacc aacctcgacg
     8821 agttgctggc gccgtggaag cgcacgcccg agaacggcga gttgctgcgc ccgatccgcc
     8881 gcgtgttcca caccctcaag ggcagcggcc gcctggtcgg tgccaagacg ctcggcgagt
     8941 tcagctggca cgtcgagaac atgctcaacc gcgtgctcga tggctcgcgc ccggccagcc
     9001 cggcggtcgt ggccttcgtc ggccagaccc gcgcggcgtt gccgcagctg cttgccgccc
     9061 tgcgcggcga agcctccggt gcgctcgaca tcgcctcgat ggaagccgtc gccgaccgcc
     9121 tggccgcggg cgaagacgtc gtgctggaca cgctcggcac cgcgccggtg gccaaggtcg
     9181 cggagcctgc ggtcgacacc gcacccgccg tcgcggcgga caccgtcgtc gcccacgtcg
     9241 atcccgtcct gctggaaatc ctggggtccg aggtcggcgg ccacctggaa accgtggatg
     9301 catggctggc ccaagcgcgt gccggcgacg ccagcgtcaa cgacggcctg ctgcgcgcga
     9361 tccacaccct caacggtgcg ttcgcaatga ccgaagtgcc gtcggtgacc gcgttcaccg
     9421 cgccggccga gggctacgtg aaacgcctgc tggccgccaa cgcgccggcc gacgccgacg
     9481 gcatcgccat ccttggcgag gtcgccgacg cggtgcgcac gaccaccgcg gcgctgcagt
     9541 ccacgccggc ccaggtgccc gtgtacgccg cgctcgccgc gcgtgtcgcg tccctgcgcg
     9601 aaaccctgcc cgaagtcagc ctgcccttca tcgatgcgaa tgccgaggcc gcccgcaagg
     9661 cggccgagga agccgaagcg gcggaagccg agcgcatcgc ggccgagcat gccgaggccg
     9721 agcggctcgc cgcggaagaa gccgcgcgcc tcgaagccga acgcgtcgag aacgagcggc
     9781 tggaagcgga acgcctggcc gcggaagaag ccgaagcggc gcggctggcc gcagagcagg
     9841 ccgaagccga gcgcatcgcc gccgaggaag cggcggcgcg cgcggaggcc gagcgcctgg
     9901 aagccgaacg cctggaagcc gagcggatcg cggcgttgga agccgaggcc gcgcggatcg
     9961 ccgcgatcga gtccgaagcc gagcgcctcg cggcacagga agcggcccgc ctcgaagccg
    10021 agcgcgtgga agccgaacgc cttgccgccc aggaagccgc gcggatcgag gaggaacgcc
    10081 tggaagcgga gcgcatcgcc gctgccgaag ccgaggaagc ccgcctggcc gccgaacgcg
    10141 cggaagccga gcgcctggcc gcggaagaag cggcccgcct cgaagcggag cgtgtggaag
    10201 ccgaacgcat cgctgccgaa gaagccgagg cggcgcgtgt cgccgccgag caggcggaag
    10261 ctgcacgcat cgccgaagaa gaagctgcgc gcgcggaagc cgagcgcgtt gccgccgaag
    10321 aagccgcacg cctcgaagcc gagcgcgtgg aagcggaacg catcgccgcc gaacaagccg
    10381 aggccgcacg cctcgaagcc gagcgcgccg aggccgatcg ccaggaagcc gagcgcatcg
    10441 ttgcggcccg ccaggaagcg gatcgccagg aagcggcgcg cctcgccgag gcccgcgccg
    10501 aagccgcgcg tgtcgaagcg gagcgtcgcg ccgccgaaat cgcggcggct gccaaggccc
    10561 gcgaacaggc ggcattggcg gcactgctgt cggccgcgac ctcggccgag gatccggacg
    10621 aagccctcga catggacggg ctggacgcgg agctggtcga catcttcgtg gaagaaggcg
    10681 gcgacctgct cgaccattcc gacggcttgc tcgcgctcct gcgtgacgcg cccgaggatc
    10741 gcgaggcgat ggtcggcctg cagcgcgacc tgcacaccct gaagggtggc gcccgcatgg
    10801 ccggcatcat gccggtcggc gaactcggcc actcgatgga aaccctgctg gaagcagtgg
    10861 tggcgcagcg tgccgaactg ggccgcgatg gcgttccgct gctcgagcgt ggcttcgacc
    10921 gcctgcacgg catgatcacc cgtgtcgccg cgcgtcgcgc ggtcggcctg ccggttggcc
    10981 tgatcgacga actgaacgcc cgcgcggaaa ccccggtcgc gccgatcacg gaagcggccg
    11041 acgacgggct ggtgttcgtg cccgcgccgg cgccgaagat cgaactcaag ccgctgtccg
    11101 caccgatgct gcaggacggt ttcggcgacg acgacgacat cggcatccgc gcaccgcagg
    11161 agcaggtgcg catccgcgcc gacctgctcg accgcctggt gaactacgcc ggcgaagtgg
    11221 cgatctaccg ctcgcgcctg gaacagcagc tcgcggcgtt ccgcggcacc gcgcaggaaa
    11281 tggagcagac caacatccgt ctgcgcgacc agctgcgccg cctcgactcg gaaacggaag
    11341 cgcagatcat cgcccgctac cagcgcgagg gcgcggccgg caacgagacc ttcgatccgc
    11401 tggaactcga ccgcttcagc accctgcaac agctgtcgcg cgcccttggc gaatccgcgg
    11461 ccgacttgca ggccctgcac gggtcgatgg aagacctgac ccgccagtac gaaaccctgc
    11521 tgctgcagca gtcgcgggtg agctccgacc tgcaggaagg cctcatgcgc acgcgcatgg
    11581 tgccgttcga gggccttgtc ccgcgcctgc gccgcgtcat ccgccaggcc accggcgaga
    11641 ccggcaagca ggcgcagctc aagctcgacg gcgcgcaggg cgaactcgac cgcagcgtgc
    11701 tggagcgcat gaccgcgccg ctggagcaca tgctccgcaa cgcgatcgcg catggtttgg
    11761 aaacgccgga agcgcgccgc aaggcgggca agggcgacga aggcacgatc cgcgtcgcgg
    11821 tccgtcgcga aggttcggaa gtcgtgctgc aggtcggcga cgatggcgcc ggcctggatc
    11881 gcgccgcgat ccgcacccgt gcgatcgaac gcgggttgat caagcccgat gcgatcctct
    11941 ccgacggcga cctcgacgtc ctcatcctcg agccgggctt ctccaccgct gaaaccgtca
    12001 gccgcctggc cggccgcggc gtcggcatgg acgtggtcgc cagcgaagtg cgccagctcg
    12061 gcggctcgct ggacatcgcc tcgaagccgg gcgaaggcac caccttcacc ctgcgcctgc
    12121 cgcagacctt ggcggtcacc caggcggtgt tcgtcaagat cggcgacacc cagtacgcgg
    12181 tgcccatcgc ctcggtgcgc ggcgtgggac gcatcagccg cgacgagatc aacaagccgg
    12241 atgcggtcta ccgctacggt ggcgaggact acgcgctgca cgacctcggt agcctggtcg
    12301 gcagcaccac ggtgaaggcg gaaggtcagt tgcagatccc gctgctgctg gtgcgctcgg
    12361 gcgacctgcg cgcggcggtg gcgatcgacc aggtcgtcgg caaccgcgaa atcgtggtga
    12421 aggcgggcgg accgcagctg gcatcggtgc cgggcatctt cggcgccacc atcatgggtg
    12481 acggccgggt ggtggtcatc ctcgacatcg ccccgctggt gcgtcgcttc gccgcgatgc
    12541 cgcgcgacgt ggcgccggtc gagcaggtgg tggaggcgcg ccgggtgccg ctggtcatgg
    12601 tggtcgacga ctcgatcacc atgcgcaagg tcaccggccg cgtgctggaa cgccacaact
    12661 tcgaggtcgc gaccgcgaag gacggcatcg atgcgctgga acggatggcc gacatcatcc
    12721 cggacctgat gctgctcgac atcgaaatgc cgcgcatgga cggctacgag ctggcgaccg
    12781 cgatgaaggc ggatccacgc atgcgcgacg tgccgatcat catgatcacc tcgcgtaccg
    12841 gcgacaaaca ccgccagcgg gcgatggacc tcggcgtcga gcgctatctc ggcaagccgt
    12901 accaggaagt cgagctgatg cgcaacgtgt tcgagctgct caagctggag cgcgccggtg
    12961 acggtcagct gcaatgaacc gcaagcgcgt ggtcgtgctg gcgcgcgcgg gggctgcgcg
    13021 cgaccgcact gaggccgcga tcgtgcaggc cggcgcggag gttgccgccc tgctcgatcc
    13081 ggcgacggcg gccgaggacg atgtgcgcgc ggcgtcgccg gacgtgctgc tggtgatcct
    13141 ggatgcgctg gtcgagcagg cgctggatcg gttcgatgcc gtgctcggcg atcccgcgct
    13201 ggaaatcctg ttcgacgatg ccgacgtcgc cgccaaacgc gatgcgtggg aaaccgcgcg
    13261 ctgggcgcgg cacctgcagg ccaagctgca cggccacgac gacgtactgc ctcccgtccg
    13321 ggatgccgac accacggcgc cgacctcgtt ggaggccgag ccgaaggggt tccaggcgga
    13381 gatggatgcc ttgagcctgc aggtcgcggc catgcccgac gtgccgcagt cgcgcccgcc
    13441 ggcctcgtcc agcgcgcccg gggcggtggt gatcgcggct ggcgtgggcg gtcccgacgc
    13501 ggtgcgccag ttgctgggcg gcctgccgcc gcagttcccg cgcccggtgg tgctgcgcca
    13561 gcggatcgag ggcggccaat acgacaagct ggtccggcag atgcagcgcg cggcgcagat
    13621 gccggtggca ttggcgcagg ctggcgatgt ccttcagccg ggcaccgtgt acgtgctggc
    13681 ggatggcctg gacctccagg ccgcgccggc cggactggtc ttcgcgcccg ccgacggcga
    13741 gccaacctat gccgcgttgc gcgccgagga cagcgccctg ctgctgctca gcggcgccga
    13801 tgtcgccttg ctcgaccagg ccatggcgat gcgctgggcc ggtggcctgg tcgtgggcca
    13861 gtcggccgac cattgtttcg atccagccgc cagcaatgca ctggtggctc ggggtggcga
    13921 ggcgctcggt cttgcccaac tcgcccaaaa actgctgcaa cgctggcccg cctgaggttt
    13981 cccatgtccg tccccatgca gactgcccct gaccagatca tccgcggcgt gctcatccag
    14041 gtcgccaatg cgcgcctgct gttgcccaac gcggcgatcg ccgaagtgtt gtcctacgcg
    14101 gacccggagc cgatcgcgga tgcgccggac tggttgctgg ggcgtacgcg ctggcgcggc
    14161 tggcaggtgc cgctggtggc cttctcgcgc ttcaccggga tcgccgacga gaagggcgga
    14221 ttgggcagca aggtgatcgt gctcaaggcg ctgggcggtg acgccaagcg tccgttcttc
    14281 gcgctgctga cccagggctt cccgcgactg gtgacggtga ccgaaaccac gctctcggcc
    14341 gagggcgacg acacggaatt gctggaaggc gtgctgagtc gcgttcgcct caacgatgac
    14401 gatgcgctgg tgccggacct ggctgtgatc gaggaacaga tcgccgcggc gctcgccgcc
    14461 gcggcctgat cgcggcgacc gctcagccca ggtcgccgca ggcggcctgc agggccgcga
    14521 tcgcggcgat cccggcggtt tcggtgcgca acacgcgcgg ccccaacttc agcccctcgt
    14581 aaccggccgc gaccaggtgt tcgcggtcgc gcggcgacca accgccctcg ggcccgatcg
    14641 cgagggtgca ggcgtgcagc tggataccgc gcagcgatga cagcgatgcc tccgcgaacg
    14701 ggtcgaggat gaaaccgcgg ccctcgcgca tggccgccgc ctgcgccagc gactgtggtg
    14761 ccgcgacctc cggcaacgtc gcacgaccac tctgctcgca ggccgacacc acgatcccgc
    14821 gccagtgcgc cagtcgcttg tccgcacgct gcgcgtccag cttcacctcg ctgcgatcgc
    14881 tgctggccgg caggatccgg gcgatgccga gttcggtcgc cttctgcagg atccagtcca
    14941 tcttctcgcc gcgggcgatg ccctgcagca gggtgatgcg cagtggcgat tcgttgtcga
    15001 tgcggcgcgc cgcgacgatc tccgcgcgcg cttcgcgctt gccgatgatg gtgatccgcg
    15061 cgtcgtaatc gtgtccgtcg ccgttgaaca gcacgcaggc atcgccgacc tgcaggcgca
    15121 gcacccgcag caggtgcgcg gcgacgtcct ccggcaacgc gacctcgatg ccggcctgca
    15181 acggcaggtc gacgtggctg cgggtcaggc gcatgcggtg gcctccagga tggcgtcgcg
    15241 ggtcacccgc gccagcaatt gcagttgctc gtcgtcgatg cagtacggtg gcatccagta
    15301 cagcgtgtcg ccgagcggtc gcagcaccac gccctgccga agggcggcgc gatacgcgcg
    15361 caatccgacc cgcaacgcgg gatcgaatgg cgtgcgcttg tcgccaccct gcgtcaattc
    15421 gaacgcgacg atcatcccgg cctggcgcac atccgccatg tgcggcagcg ccgccagtgg
    15481 cgcggcgagt cgtgacatgc gcgccgccgt cgcgcgattg cgcgcgagga tgtcgtcgga
    15541 ttcgaagatc gccagtgagg ccagcgcggc ggcgcaggcc agcgggttgc cggtgtagct
    15601 gtgcgagtgc aggaaggcgc gctcgcgcga atcgtccagg aagccgtcgt agatcgcctg
    15661 cgtgcacagc accgcggcca gcggcaggaa gccgccggtg agccccttcg acaggcacag
    15721 caggtcgggc tgcacaccgg cctgttcgca cgcgaacagg ctgccggtgc gcccgaagcc
    15781 gaccgcgatc tcgtcggcga ccaggaacac gccatgcgca tcgcagagtt cgcgcgcgcg
    15841 gcgcaggtac cgtgggtcgt gcatgcgcat gccgccggcg cactgcacgc gcggctccag
    15901 gatgagcgcg cagacctcgc ctggatggcg ttccagaagg tcgcgcaggc cgtcggccgc
    15961 gcggtcggcg cagtcgatgg cggtttcgcc cggcccggcc gcatagctgt ccggcgaggg
    16021 cgcgaacagg cattcggcga gcagcggcgc atacacgcgt cgatacagcg ggatgtcgcc
    16081 caccgacaac gcgccgatgg tctcgccgtg gtagccgttc tccagggcga cgaacttgct
    16141 gcgccggtcc tcgccgcggt tgcggaacca gtggaaggcc atcttcagcg cgacttccac
    16201 gccggccgac ccattgtcgg cgtagaacac cttcgacagt gcggcgcgcc cggattcgcg
    16261 cggtgcgatc gccagcaatt tctcggccag ctcgaccgcg ggttgatgcg agaagccggc
    16321 caggatcacc tgctccagcg tcatcgcctg ctcggcgatg gcgcgggcga tccgcggctc
    16381 ggcgtggccg aacaggttgg tccaccaact gctgaccgca tccaggtaac gccggccgtc
    16441 ccggccgacc agccacgccc cttcgccgcg ctcgatcggc accagcggca gcacgtcggg
    16501 gtgctcgcgc atctgcgtgc acgggtgcca gagcacgtcc aggtcgcgtg cccgccatgc
    16561 atccgcggcg gctatcatgt cggcatcgtg aaatccttcc attcgcccat tatcgccgcg
    16621 ggaggcgtgc catgagccga cccctgccca tcatccacgg catcaccgag cacgacgaga
    16681 gcccgtaccg gatggagcgg ctcgacctgg agttcagcaa cggcgagcgc cgtcgttacg
    16741 aacgactgca cggccgcggc cacggcgcgg tcgcggtggt gccgatgctg gatgccgaga
    16801 cggtcctgct ggtccgcgaa tacgccgccg gcgtgcatcg ctacgaactg ggcctggtga
    16861 agggccgcat cgatgccggc gagagcccca tcgaggccgc caaccgcgaa ttgatggaag
    16921 aggccggcta cggcgcccgc gatctccagg tgctgcgcag catcaccctg gcgccggtgt
    16981 acatgagcca ccaaacccat ttggtgctgg cgcgcgacct gtatccgcag cgcctgcccg
    17041 gcgatgaacc cgaggaactg gaagtggtcc cgtggaagct cgacgacctg ggcgagctga
    17101 tcctgcgcga ggatttctcg gaaggccgca ccatcgcgtc gttgttcatc gcccgcgagt
    17161 ggctgaggca gcagcgatga tcctgtcgaa cgaacaacgc gatgcggtca tcgcgctggc
    17221 gcgcgccgcc gccgccgaga tcctggcggt gtacgagagt ccgttcgcca tcgagcacaa
    17281 ggacgaccgc tcgccgctca cggccgccga tctcgcatcg catcgctgca tcgtggcggg
    17341 cctgcacgca ctgacgccgg acatcccggt gctctcggaa gaatcgaggg acctcgacat
    17401 cgccgcgcgt cgcgaatggt cgacgttctg gctggtcgat ccgctcgacg gcacccgcga
    17461 attcatcaag cgcaacggcg aattcaccgt caacatcgcc ctggtcgagg acggcgtggc
    17521 gagctggggc gtgatccagc agccggtgac cggcgcgctc tggcatggcg gtgccggcca
    17581 cgggtcgttc atccgcgagg gtgacgccgg cgacgtgccg atccatgcgc gcattcccgc
    17641 atcggaaccc ctgcggatcg ccgccagccg ctcgcatcgc gacgagcgca cccaggcggt
    17701 gatcgacgcg ttgcccggca gcgacgtggt gggctgcggc tcgtcgctga agttctgccg
    17761 gatcgccgat ggcggcatcg acctgtatcc gcgcttcggt cccaccagcg agtgggacac
    17821 cgccgccggc caggcgatcc tggaaggcgc gggtggcgcg gtgctggatc cgcgcggccg
    17881 cccgttccgc tacaaccagc gcgacacgtt gctcaatggc gacttcatcg cgttgggcga
    17941 catcgccctg cgcgaccgct tgcccgcatg agcgaaccgg gtccggtcga atcacgccag
    18001 gtcgagtcgc tgctcgcgat catggcgaaa ctgcgcgatc ccgacggcgg ctgtccgtgg
    18061 gacctggcgc aggatttctc gaccatcgcg ccgtacacca tcgaagaggc gtacgaagtc
    18121 gccgatgcga tcgaccgcaa tgacctggtc gacctgaagg acgaactcgg cgacctgctg
    18181 ctgcaggtgg tcttccatgc gcagatggcg aaggagcagg gtgcgttcga gttcgccgac
    18241 gtggtcgcgg cgatctgcga caagatgacg cgccggcatc cccacgtatt tgcggaggag
    18301 gcgcgcaagg acgatgcaga cgcgcaactg cggcactggg aagacctcaa gcgcgccgag
    18361 cgcgcggcga aaggcgatgc cgatgcgtcc gcactggccg gcatctcgcg cggcctgccg
    18421 gagtggcagc gcgcggtgaa gctgcagcac aaggcggcca aggtcggctt cgactggccc
    18481 ggccccgaac ccgtgctgga gaaattgcac gaggaaatcg aggaagtgcg ggtggaattc
    18541 gcggccgtcg cggcttcacc cgacgatgcc gccgcgcgcg accggctgga ggacgaactc
    18601 ggcgacatcc tgttcgtctg cgccaacctg gcgcgccacg cgaaggtcga catgggtagc
    18661 gcgctgcgcc gggccaacct gaaattcgag cgccgcttcc gcgcgatgga agcgctggcg
    18721 gccgcggatg gcggccttgc cgggcaatcg ctggaagcgc aggatcgcta ttgggatcgc
    18781 gccaaggcgg cggagaaggg agcggcagca tgagcagcga cgcgcagcac gagttcgcca
    18841 gcttcagcga gttctatccg ttctacctgg gcgagcatcg caacaccatc tgccgtcgcc
    18901 tgcatttcac cggcaccagt ttcgcgctgg gcttcatcct gctggccgtg accagcggca
    18961 acgcgtggtg gttcctcggt gccttgctgt cgggctatgc attcgcgtgg atcgggcatt
    19021 tcttcttcga gaagaatcgc cctgccacgt tcaagcaccc gttctattcc ttcgccggcg
    19081 actgggtgat gtacaaggac atcctggtcg ggaagatccc gttctagcgg gtcggcatga
    19141 gaggtgtccc ggtcgcgcgg atgccatgca gcaagcgcac tactccagga agatcaacca
    19201 cgccgccgcc gcgatcagcg cgaacccggc ccagtggttc cacttcagtg gctggcccag
    19261 gtaccagatc gagaacccgg cgaacaccag caaggtgatc acctcctgca tgcccttcag
    19321 ttgtggcgcg ctgtaaagcg cgctgcccat gcggttggcc gggaccatca gcaggtactc
    19381 gaagaacgcg atgccccaac tcgccaggat cactttccag agcgcgacat ccttgtgctt
    19441 gaggtgcgcg taccaggcga aggtcatgaa cacgttggag aacagcaaca gcaccggcgg
    19501 caggaagcgg tcgtagggca tggggggcag gggcgacgga aagttgccga cagggtagtc
    19561 cttcacgtcg cttccactcc gaacccggca tgtttcacaa agctgaagac atgtatggag
    19621 catgtatgcg ggcacaggaa caagcgggat tggtcctcat cgtcgaggac aaccgcaata
    19681 tttcggaaat ggtcggcgaa tacctggaaa gccggggctt cgaggtggat tacgcctcgg
    19741 acggcctgga cggttatcgc cttgcggctg agaacaccta cgatgtcatc gtcctcgacc
    19801 tgatgttgcc gcgcctggac ggcatcgagg tctgcaagcg cttgcgcaac gaggcgcgca
    19861 agtccacgcc ggtgttgatg ctgaccgcgc gcgacaccct cgatgaaaag ctcaccggcc
    19921 tgtcgtccgg cgcggacgac tacctgacca agccgttcgc gatccaggaa ctggaagcac
    19981 gcctgcgcgc cttgatccgc cgcgaacgcc gccaggtcgg ttcggaagtg ctgaaggtcg
    20041 ccgacctggt gctcgacccg gcttcgctgc gcgccacccg cgggggcagc gaactgcagc
    20101 tgtcgccgat cggcctgcgc ctgctcacca tcctgatgcg cgagtcgccg cgggtggtca
    20161 cccgccagga gatcgagcgc gagatctggg gcaacagcct gccggactcg gacacgttgc
    20221 gcagccacct gtacaacctg cgcaaggtca tcgacaagcc gttcgaccgc ccgttgctgc
    20281 ataccgtgca gagcgccggt taccgcatcg cggacatcgc cagccagttg ggctgaacgc
    20341 gcggctttgc tggtggaaaa aacccaacgc catcttcgcg agatcttcct gctgcaattg
    20401 gcgatcggat gcctggtcgt ggtgctgtgc gtgctcggcg tgcaggcgat gtacggcttc
    20461 gccgcgaccc cgtgggtgtc ggcgcccgtg ctcgtggtcg cgttgggtgc cgtgatcggt
    20521 gtcacctgga tgagctatcg cgcgacacgg cgggtcatcg agccgatgga ctggttgctg
    20581 cgcgaggtcg cgcgctggga tccgcaacgc ccggataccg cggcgctcgc gccggccaat
    20641 gtgccggccg cggtgcaggg cgacgtgcgc aagatggccg aagccctgca tgcgctcggc
    20701 cagcggctcg atacctacgt cacccgcgag cgcgacttca cccgcgatgc cagccacgag
    20761 ttgcgcacgc cgctgacggt gatccgcgtg gcgaccgacc tgattggcca cgacgaaggc
    20821 ctctcgacgc gctcccggcg ctcgctggcg cgcatccagg gtgcggcgga gtcgatggag
    20881 tcgctgatgt ccgcgctgct gttgctcgcc cgcgacagcg aggtgccgct ggagacggaa
    20941 gacttcgacg tgcgcgaggt ggtggagcac gagatggaga aggtgcgctc gctgctcgag
    21001 gacaagggcg tgtcgctgct gctggacgtg caggcggaac cggttctgca tgcgccgcca
    21061 cgcgtattgc aggtcatcgt gtgcaacctg ctcagcaatg cggcgcgctt caccgatgcc
    21121 ggcagcgtgc aagtgcggct cctgcaggat cggatcgaga tcgaggacac cgggatcggc
    21181 atggatgcgg ccgcgctggc gcgtgcattc gagcctttct atcgggccag catcgagcag
    21241 ccggtcggcc ctggcttggg cctgtcgatc gcacagcggc ttgggcatcg ctgcgggtgg
    21301 ccgttgcagc tggccagcgt gcccgggcag gggacgcgcg ccagcatcct gttcggggcc
    21361 cgcaaccggg accaatgacc cgttgcggca ggtgcgtggc cggttagcat gcgcgcgctg
    21421 tcaaccgcgc cggcatccgc gcgttcccga ccctttccgg actcccctcc ccgcatgaac
    21481 cagcctcgtc gtcgtcctgc cttccccctg cgcaagctgg gcatcgccgc catcgtcatc
    21541 gccgtcgcgg ccggcggctg gtatttctgg ggcaagcgcg gggcgtcggc cgatggcggc
    21601 taccgcaccg agaccgtgca gcgcggcgac gtgcgcgtgg cgatctcctc gaccggcacg
    21661 ctcagcgcga tctccacggt caccgtgggc agccaggtgt cgggccagat caccgaagtg
    21721 ctggtcgatt tcaatgaccg cgtgaccaag ggccaggtgc tggccacgat cgatgcgtcg
    21781 acctaccagg cacagatcga gcagggctcc gcgcagatcg ccagcgcgca ggcctcgttg
    21841 cggcaggcgc aggcaggcct gcgcaacgcg caactggact acaaccgcaa ggccgacctc
    21901 ggccgccagc aattggtcag caagagcgac gtcgacctgg cgcgttcgtc gctggaacag
    21961 gcgcaggcgc aggtgaactc cgcgcaggcg cagatccgcc agcagaccgc ctccacccag
    22021 accacccggg tcaacctgca acgcacggtg atccgctcgc cggtggacgg ggtggtgctc
    22081 acccgcacga tcgagcccgg ccagaccgtg gccgccagcc tgtccgcgcc ggaactgttc
    22141 accatcgccg aggacctggc gaagatgaag atcgaactgg ccgtggacga atccgacatc
    22201 ggccaggtga aggtcggcca ggtcgtcagc ttcagcgccg atgccttccc ggagcggcag
    22261 ttcaagggcg tggtcgacca ggtgcggctc tccgcaacca cttcgaacaa tgtcgtcacc
    22321 tatccggtgg tggtcaccgt cgacaacagc gacggcaccc tgctgcccgg cctgacggtc
    22381 aacgccgaga tcgaagtgag caagcgcagc gatgtattga agctcgcgaa cgcggcgctg
    22441 cgcttcaagc cggccgaggg ttcgccgctg gcggcgctgc agggcgctgg cccgggccag
    22501 ggtccgggcg cgcagggcgg tcgtggcgcg ggcatgagcg aagacctgca ggcgctggcg
    22561 gccacgctcg cgctgaagcc ggagcagcag gccgcgttcg atgccgcgct ggagcagatg
    22621 aagcaacgcc aggcggagcg catggcgcag gcgccggcgc aaggccagca gggcggcaac
    22681 cgcatgttcg gcggtggccc gccgcgcatg ggtggcggcc agggcggcgg cgacgccagc
    22741 atgcaggcgc agatgcgcgc gcgcatgcgc gaccgcttca accagcagtt cgccgccttc
    22801 cgcgacaccc tggacgatgc ccagcgcgcg aagtgggacg gcgcgcgcga tgcccagctc
    22861 aacgccaagc gggtcaccat ctacaagctg gtcgacggca agccgcaggt cgcgatggcg
    22921 cgattgggcg ccagcgacgg cagcagcacc gaaatctccg gccgcgacat cgccgagggc
    22981 gacctggtca tcgcgggcga gcaatcggcg acggagagca agtgatggcc gtcggcgtgg
    23041 ccgaggcggc ggtgccggtc atccgcacgc agggcctgag caagatctac tccgccggca
    23101 ccgaagcgga agtcgcggcg ctgcggggcg tggacctggt gatcgaccgc ggcgaattcg
    23161 ttgcgatcat gggtccgtcg ggctccggca aatcgaccct catgaatctc atcggttgcc
    23221 tggatacgcc caccgccggc aagtacttct gcgatggcgt ggacgtgtcc acgctcgaca
    23281 aggaagcgct ggccacgctg cgacgcgaca agatcggctt cgtgttccag ggcttccacc
    23341 tgctcacgcg gatggatgcg accgacaacg tggcgatgcc gctgggctat gcgcgcatcc
    23401 cgccggcgca acgcaaggag ctggcccgcg ccgcgctcgc gtcggtcggc cttgcggagc
    23461 gcgccgggca tcgcccgaac gaactctccg gcggccagca acagcgcgtg gcgattgccc
    23521 gcgcgctggt caacaagccg ccgatcctgc tggccgacga acccaccggc gcgctcgaca
    23581 gcaagaccgg cgaggaaatc ctggccctgt tcaagcgcct gcgcgacgag ggccacaccg
    23641 tgatcctgat cacccacgac gcgcacgtgg cggcgcatgc cgaccgcact tacgtgatgc
    23701 gcgatggcga gctgcacgcg caaaccgaag cggaggccgc ggcatgaccc tcctcgacat
    23761 cctgcgcacg gcgttcttcg ccttgcgcgg caactggatg cgcagcgcgc tgacctcgct
    23821 gggcgtgatc atcggcatcg cggcggtgat cgtgatggtc tcgatcggcc agggcacgca
    23881 ggccgagatc gacaagatgg tgtctggcct cggctcgcag cgcctcgaca tcggcggcgc
    23941 cggcggcatg ggcccgggtg gcgcgcgcat ggcccagggc agccgctgga gcctgaccgg
    24001 cggcgacgtg gaagcgatcc gcaatgaagt cccgacggcg cagtacgcgt cgggctcgct
    24061 gcgcggcaac acccaggtgg tgttcgcgga aaacaatgcc tcgaccagct ggcagggcgt
    24121 ggaggccgac tggttcgcga tcaacgactg gaaggtggcc aggggcgacg gcttcagtgc
    24181 caacgactac ggcggcagcg ccaagccggt gatcctgggc gaaaccgtgc gcaagacctt
    24241 gttcggcgaa gaggatccga tcggccagac cgtgcgcctg gggcgggtgc cgttcaccgt
    24301 ggtcggcgtg ctgggggcga aggggcaggg cggcttcggc caggaccagg acgacatggt
    24361 ggtggtgccg ctggaaaccg cgcggcggcg cttgtcgaat tcgcagtcca tgccgccggg
    24421 cgcggtgcag gcgatttcgg tgggcgtgga cgacgccaag aacctggcca gcgcacaggc
    24481 cgagatcgag gccctgctgc gccagcgcca caagatcgaa ccgggcgcgg aggacgactt
    24541 cgcggtgcgc aatatcagcc agatcgtggc cacccgcacc gccaccacca acctgatgtc
    24601 gaagctgctc ggcgcggtcg cgggcatctg cctggtcatc ggcggcatcg gcatcatgaa
    24661 catcatgctg gtctcggtga ccgagcggat ccgcgagatc ggcctgcgga tggcggttgg
    24721 cgccgggcct ggcgacgtcc agaggcagtt cctggccgag gccatgctga tctccctgat
    24781 cggcggggtg atcggcatcg ccatcggcgt ggtcggtgcg ctggtggtgg gcaagctggg
    24841 cgacctgccg gtcgaactca atgccaaggt ggtgatgctg gccgcggcgt tctcggtcgg
    24901 taccggcctg ttcttcggct actacccggc ccgcaaggcg tcccagctgg atcccatcga
    24961 ggcgctgcgc tcccagtagc tgccggccga gccaagcccg gcaagggcag cgataacggg
    25021 gcttgtggca aaatccgcgg gtcggcacgg ccgacccacc ggatgccgtc gcccatgctt
    25081 cacaccgcca ccttcatgct gccggccgcc atcgcgcatg gctgacatct atgggcacct
    25141 gccgcggggc ccggcgggca tcatgcgcgc cgccgtctgg tcgttgcagg gcctgcgggc
    25201 ggcgtggctg cacgaatcct cgttccgtct ggaagtctgc ctgttcgtgg tgcttggccc
    25261 gctcggctgg ttcctcggcg cgactggcgt ggaacgtgct ttgctcattg gcagttgcct
    25321 gctggtgatg gcgatggagc tgatgaattc ctcgatggag gcggtgatcg agcgctacgg
    25381 cgcggaacgc cacgagctgg ccgggcgtgc caaggacatg ggctccgcgg cggtgttcgt
    25441 gctgatgatg aacgtgctgc tggtgtgggc gctgatcctc ggcccgcgct acctgtaatc
    25501 cgcgccgatt gcgcgactag aacgagaagc gaatggacct gagtttcctg gcgtcacccg
    25561 atgcatggct caccctggtg accctgagcg cgctggagat cgtgctcggc atcgacaacc
    25621 tggtgttcat ctcgatcgcg gtgtcgcgcc tgccggaggc gcagcggccg aaggcgcgca
    25681 agatcggcat cgcggtcgcc tgcgtcaccc ggatcatgct gctggtcatg ctggcgttcc
    25741 tggcgaccat ggaacaggac ctgttcgtgc tgttcggcat gggcatctcg atccgcgaca
    25801 tggtgctgat cgtgggtggt gccttcctgc tggtgaaggg cacgctggaa atccgcgatc
    25861 tgatcagcgg cggcgaggac gaggatcccc gcaccaccaa gacatcctcg gtgttctgga
    25921 tggtgattgc gcagatcgcg gtgatcgaca tcgtgttctc gctggattcg gtgatcaccg
    25981 ccgtcggcat cgcccagcac atcccgatca tggtgttcgc gatcctgctg gcggtggcga
    26041 tcatgctgct ggccgccaac ccactgggca agttcatcga cgataacccc accgtgaaga
    26101 tgctggcgct ggccttcatc ctgctggtcg gcgccgtgct gatcctggat ggcctgggca
    26161 tccatgtgcc gaagccctac atctacttcg cgatggcgtt ctcggtcctg gtcgagtggc
    26221 tgaacctgct gatgcgccgc aaggcggcgg cgaactacgt ccccggcgcg ggcgagtggt
    26281 agcggtttgc acgcaggctt aaccccgttt gccggtcact aggcgttaga gcaggtccct
    26341 cgtcttccag gagcttccca tgcgtggtct ttcgcttgtc cgtggcgcgc tgctcgcgct
    26401 ggtcgtggtg atgctcagcg gctgcggcta caaccagatc cagagcaagg acgaggcggc
    26461 caaggccggc tggtcggaag tgctcaacca gtacaagcgc cgcgccgacc tgatcccgaa
    26521 cctggtcgcc accgtgaagg ggtatgccgc gcacgaggcg caggtgctga ccgcggtgac
    26581 cgaggcgcgc gccaaggtca gccaggtcaa cgtgaatgcc gatgacgcgg cctcgctggc
    26641 gcagtaccag gccgcgcagg gcgagctcag ccaggcgctc ggccgcctga tgatggtgac
    26701 ggaggcgtat ccgaacctga aggcggacca gcagttcatc ggcctgcaga cccaactgga
    26761 aggcaccgag aaccgggtga ccgtggcgcg aggccgctac atccagctgg tgcaggacta
    26821 caacacctat atccgccagt tcccggtcaa cctcaccgcg atgatcttcg gctataagcc
    26881 gaagccgaac ttcacggtag agaacgaggc gcagatccag gatgcgccgg cggtcgattt
    26941 cgccacgccc ccccccgcgc cggtagcgcc gcctgcccct gccgtgccgc cgggcatcgt
    27001 gaacccaccg cagccggccg cgccgccggt gcagggcggc aacgccccgc aggatccgca
    27061 ggacgcaccc gcggcctgag ccgggaacga ccgccatggc ggatgtccgg atgccttcct
    27121 cgcagcgtcg cgcgttcgcg gcgctgcgtg cggtcttgtg gtgcgcatgc ctgctgctgt
    27181 ccctggtctt gccggcctgg gcgcaggcgc cgataccgac gttcgcttcg ccggtcgtcg
    27241 atacgaccaa caccctggac gccgcgacca ggcagaaact ggagcagcaa gcgttggccc
    27301 tccagcaacg caagggcagc cagttacagg tgttgatgat ccccagcacc cagcctgaag
    27361 gcatcgaggc gtatgcggtg cgcgccttcg agtcgttcaa gctgggccgc aagggcatcg
    27421 acgacggcgt gttgctggtc atcgccaagg acgaccgcaa ggcgcgcatc gaggtcggct
    27481 acgggctgga gggcgccatc cccgacatcg ccgccggccg gatcatccag gaatacatgg
    27541 tgccgaagtt ccgccagggc gactacgccg gtggcgtgga ggatgccacc gcgcagttgg
    27601 tcaagctggt cgatggcgag ccgttgcctg agccggtggc cagcaattcg accgggcgcg
    27661 acagcggcga cgaatggttg ttcgcactgt tcgctgcgtt catggcgtcg ctggtcgcgc
    27721 gctcgatgtt cggtcgattg cctgtcggac tgcgcatcct gttcaccgtc atcgctggtg
    27781 gggtggcagc gtggctgttc tcctgggtgg tcttcgtcgg tggtgtcggc gcggtcatcg
    27841 gtttcttcac cgcgctgatg ggcggtggcg gtggcggcgg cggacgttac gcacgacacc
    27901 atgactgggg tggcttcggt ggtggcagtg gtggcggtgg atggggaggc gggagttccg
    27961 gcggcggttg gtcgggtggc ggcggcatgt ccgggggcgg tggcgcctcg gggggctggt
    28021 aatggggatc acgtgatggc cggcaccctc aagcgcctgt tcacccatct gttcaccgcc
    28081 gatgcgcgcg ggctgttccc gacatcggcg atggagcgga tcgccgcggc gatcgacgtg
    28141 ggcgaaacct gccatgcggg tgaagtgtgt ttcgcggtgg agtcctcgct gcactggcgc
    28201 gacgtcctgc gcggcgtgca ggcgcgtgcg cgcgccgagg atgccttcgc ccgcttgcgg
    28261 gtctgggata ccgcggcgaa caacggtgtc ctcgtctacc tgctgctggc cgaccaccgc
    28321 atcgagatcg tcgccgatcg tgggctggcg ccgttcgtca gcgccgaaca atggcgccgc
    28381 gtctgcgaac tgatggaggg gcggctgcgc gcgggcgagc atgcggacgg cgtggtcgcc
    28441 ggtatcgaag ccgtgggcga cctgctggcc gagcatttcc cgcaggaacc aggcgcgccc
    28501 gatgaagacg agctgcctga ccagccggtc ttcctctgat ctccgcacca ggcgccccgg
    28561 caatcaatcg cgatggcgag gccgtcgggc agcacgcgta gcggcatttc gagccgcgta
    28621 acgcacgccc ggcggcaggc gccatcgcag cgacattccc catctgcggc acaataggca
    28681 gatgccttac ctccatcaga tcgaccccat cgccctcgac ctcggaacct ggaacctgcc
    28741 gctgctgggc agcgtgcacc cgcaggtcca ctggtacggc gtgatgtatt tgctgggctt
    28801 catcgtggcc tggtggctgg gccgccgccg catccgcgcg ggccggctgc cgggcgtgga
    28861 cgagaacggg ttcggtgacc tgatgttcta cgcgatgctg ggcgtggtgc tcggcgggcg
    28921 catcggctac atcctgttct acgacttcgc cagcttcgcc gccaatccgg cgatggtgtt
    28981 caaggtctgg gaaggcggga tgagtttcca cggcggcctg ctcggcgtcc tcgtcgcggg
    29041 tgcgtggtgg tcgcgccgcc agcgcctgca tttcttcgac acggtggatt tcgtggcgcc
    29101 gctggtgccg gccgggctgg gattcggtcg cgtgggcaac tggatcggcg gggagctgtg
    29161 gggcaagccg acccagggca ccgcgttcga cggctggggc gtggtgttcc cgcgcgcgtt
    29221 gcccgacccg ttcgcctcga tggacgcggc gacgctgaaa cagcagttcg atgccggcat
    29281 cctcgacagc tttgcccgcc atccttcgca gctgtaccag gcgacgctgg aaggactggt
    29341 gatgttcgcg gtactggcct ggtattcgcg cacgccgcgt ccgcgctacg ccgtctccgg
    29401 cctgttcgcg ctgctgtacg gttgcttccg cttcctggtc gagttcgtgc gcgagcccga
    29461 ccagcaactg ggctatctgg cgttcggttg ggtgaccatg gggcaggtat tgagcctgcc
    29521 gctgatcctg ctgggcctgt tcctgtgctg gaagtcgcac agctcgccca ccttgcaacc
    29581 gcaacccgcg gacacggcga agtgacgcac tgagccatgc gccaatacct cgacttgctg
    29641 cgccatgtgc tggaacacgg caccgagaag gcggaccgca ccggcaccgg cacccgcagc
    29701 gtgttcggct ggcagatgcg cttcgacctg aacgatggct tcccgctggt caccaccaag
    29761 aagctgcacc tgcgctcgat catccacgaa ctgctgtggt tcctgcaggg ccagaccaac
    29821 atcgcctacc tgaagcagca cgatgtcggg atctgggacg agtgggcgga tgccgacggc
    29881 gagctcggcc cggtctacgg caagcagtgg cgcagttggg aaggcgcgga tggcgaggcc
    29941 gtcgaccaga tcgcgtggct catcgacgag atcaagcgca atccggattc gcgccgcctg
    30001 atcgtcagtg cgtggaacgt cgccgacctg ccgaagatgg ccttgatgcc ctgccactcg
    30061 ctgttccagt tctacgtggc ggatggaaaa ctcagctgcc agctgtacca gcgcagcggc
    30121 gacatcttcc tgggcgtgcc gttcaacatc gccagctacg cgctgctcac ccacatggtg
    30181 gcgcaggtct gcggcctcgg cgtgggcgat ttcgtgcata cgctgggcga cgcgcacctg
    30241 tactcgaacc atgtcgagca ggcgcgcaag cagctctcgc gcagcccgcg cgcgctgccc
    30301 acgctcgcgc tcaatcccga ggtgcgcgac atcttcgcct tcacctacga ggacatcgcc
    30361 atccacggct acgatccgct gccggcgatc aaggcgccgg tcgcggtctg aaccacgtcg
    30421 gtgtcttcca tgcatgtttc cctgatcgcc gcgctggatc gcgacttcgc catcggcaag
    30481 ggcaatgcct tgccctggca cctgccggat gacctcaagc gcttcaaggc gctgactttg
    30541 ggcaagccga tcctgatggg ccgcaagacc gcggaatcgc tgggccgcgc gctgcccaag
    30601 cgacgcaacc tggtactgac ccgcaaaggc cgcgttccgt tcgacggcat gcaggcggtg
    30661 tcgtccatcg atgacgcgat ggcgatcgcg cggcacgatg gcgacgaact ctgcatcatc
    30721 ggcggcggcg aggtgtatgt gctcgcactc ccgcatgcga cgcgcctgca tctcacccat
    30781 gtcgacacgc gcgtcgaagg cgcggatgcg ttcttcccgc gcttcgatgc cgcagcgtgg
    30841 cgcgaggtgg gtcgcaccgt gcatcccgcc gatgcgatgc atgcgttcgc cttcgacttc
    30901 gtggattacg tgcgggcagt cggcaccgac tgaccttgcg gccgcggttt tcgcgttggc
    30961 ccgggcatcc ccgaactgcc cggatcgcca tgcgcctctt gccagtcttc cttgctgcct
    31021 gcctgttcgc ggcaacggcg tgttcccgtt ccgaggctcc cgccgcctca tcagctgccg
    31081 tggccaagcc cgcagtcgcg acgacacccg gaaccgatgc gcaggaagcg gccaccccgc
    31141 cggccatcgc gcccaacctg ctgtcgttcg ccaacggcac catcatgccg ctgcccacgg
    31201 acgcgctgct ggtcaccgcg ccgatcctga tgatcgacgg atcctcgatc cagaactggg
    31261 tcggcgatgg caagccgcag tcgttcgtgt tcgagctgcc cgaggccgcg accctgcgca
    31321 tgctggagtt cgacaacgac acatccggca tgggtggcgt ggatgcgggg atcaaggacc
    31381 tcaccgttga ggtctcgacg acctccgcca ccgagggctt ccagcaggtc ttcgcgggta
    31441 gcttggccaa aggcgtcaac ggccagcgct tcgacatcgc caagccggtc ccagggcgct
    31501 gggtacgcgc caacttcgcc tccaaccacg gcggcgagtc gtattcgctc gccgaactcc
    31561 gcgccttcgg cgatgcaccg ccggccgtcc tgtcgaccga tgccagcggc agctacgaga
    31621 cggtctgggg tacctggaat atcacccagg cgggcaccac gatcagcggc tgcttccagc
    31681 ccggcggctt gtcgacccag ccggccacct tcagtggcgg catggagggc aacatcgcgc
    31741 ggatccgcta catcgagacc gatgatgccg gtgcaccacg cgaagcacgc gcgttgctgc
    31801 tggtgttcgc ccgcgacggc aagcgcttct tcaccgccgg cgtggatggc gaaagcctgt
    31861 ccgactacgg cgaggtcaag cgcattgccg ccgaaccggc gacctgcccc gggcgtgccg
    31921 cgaatgcgcc agcgaggacg atggccgaca cgctggccaa ggaggggcgc gtggccgtgt
    31981 acggcatcaa cttcgatttc gatgcggcga cgctgcgccc ggaatcgacc gtggtgctgg
    32041 agcaggtcgc cggcctgctg cgcgatgatc cggcgctgcg catcaccatc gaagggcata
    32101 ccgacgatgt cggcggcgct gcctacaacg acgcgttgtc cggcaagcgc gcgaattcgg
    32161 tcaaggattg gctggtggcc gcgggcatcg atggcgcgcg gctggaggcg gtaggcaagg
    32221 gcgccggctc gccggtggcg accaatggca acgacgtggg tcgcgcgcag aaccggcggg
    32281 tggaattggc caggcgctga gcgcacccaa ccgaccgggg aaatgctcag gcctggtcgc
    32341 ggcgctgcgc gcctggcggc ttgtgcgggc gattgcctgg cttgggtcgg ggcggacgtt
    32401 gccggggcgg cgtcgcgggc acgtcgcgcc cgggcacctg cactacgtgc agggtttcgg
    32461 catccagctg caacgcggtg agcttgccgc cccagaccgc gccggtatcc agcgcatgca
    32521 cgccctggcc gatgaacagg cccagcgtgc tccagtgccc gcagacgacc ttgaggtcgc
    32581 ggttcgcgcg gccgggcacg gaataccagg ggtacaggcc cggtggctgg gtgcccggtt
    32641 cgcccttgtg ctcgaaagcg atccgcccgc gcgggctgca gtagcgcatt cgggtgaaga
    32701 tgttgatgat cgcgcggtcg cgctcggcgc cgcccagccc cggtgaccag tccgggccgt
    32761 cgccgtacat gttcttcagc aggcgccggt agttgtcccc gtggaggcgg gattcgactt
    32821 cgcgcgcgtg tttttcggcg agctgggtgg tccacttcgg ggccagcccg gcgtgcacca
    32881 tcatccagcc gagatcgcga tccacgtgcg ccagctcgcg catccgcagc cagtccagga
    32941 gctcgcgcgc gtcctcggcg aacagcacgc cctgcaggtc gggattgacc ttgcgctgct
    33001 cgtctggcga gcgctcgccg atggccagca gcgacaggtc gtggttgccc agcaccacgt
    33061 ggctcacctc gcgcagcgaa tgcaccaggc gcagggtttc gatcgattgc ccgccgcgat
    33121 tcaccaggtc gccgcagaac cagatgcggt ccacggccgg gtcgaagcgg atcttctcca
    33181 gcaggcgctg ggtgacgtcg tagcaaccct gcaggtcgcc gatggcccag gtcgccatgc
    33241 ccgggcctca gtgcagcgtg cgcgggatgg acagcgtgaa cggcgggatc ggcgcggcga
    33301 agcgggtgcc atcgtctgcc agcacgtcgt aactgccgcg catggtgccg atgtcggtct
    33361 ccagtaccgc acccgaggtg tactcgaagc cttcgccggg acgcagccac ggttgttcgc
    33421 cgaccacgcc ttcgcccacg acttcctgga ccttgccgtt gccgtcggtg atcagccagc
    33481 ggcgtccaag cacttgcgcg ggcaccttgc cgtcgttgcg gatgtgcacg gtgtaggcga
    33541 acacgaagcg gccttcctcg ggcacggatt gttcatcgag gaaacgcgtg gcgacttcga
    33601 tgtcgaactg gtaatcggtg ttgctcatgc gtgcattcta gcggcgatgc gcgccacatc
    33661 gcatgaaagc ggcctcagcc ctgcgcgagc agattcgcca ggcgcacgaa cccggcgacc
    33721 tcgacctgtt ccgcgcgcgc atcggcgcgc aggccggcgg cctcgatcgc ggccacgtcg
    33781 gccacgccgt tcagcgcgtt gcgcagtgtc ttgcgccgct gcccgaacgc ggcacgcacc
    33841 acgttggcga agacgcggcg atctgccacc aggatcgact cggcgggctt cggaacgagt
    33901 cgcaccaccg ccgaatccac cttcggcgca gggcgaaacg cgcccggcgg caccacgaac
    33961 aacggagtga cgttgcagta cgcctgcagc atcacgctga gccggccgta gaccttgctg
    34021 ccggggccgg cggccatgcg ttcgaccacc tccttctgca gcatgaagtg catgtcgcgg
    34081 atcgcctgcg cgtggtccag cgcgtggaac aggatcggtg aactcaggtt gtagggcagg
    34141 ttgccggcga ggcggatctt gccgcccgct ggcgccagct ctgcggcaag cgcgctgaaa
    34201 tccacgccca gcacgtcgcc ctcgatcagg tgcagcgcac catggtcgcg ggcggcggcc
    34261 tgcagcggcg cgtgcaggtc gcggtcgaat tcgatcgcgg tgatcgcgcc gtgccgcttg
    34321 agcaagggga acgtcagtgc accctggccc gggccgatct cgacgaccgc atcgcccggc
    34381 ttcggatcga tcgcctgcac gatcttgtcg atcaccccac ggtcgtgcag gaagttctgg
    34441 cccaagtgct tcttggccgg ttcggtgaag ctggcggtca tgcgtgcatc cggtggcggg
    34501 cgaactgcgt gcaggtgtgg atcgcggcga acaggctgga gggatcggcc atgcccttgc
    34561 cggccaggtc cagcgcggtg ccatggtcca ccgccacgcg tggatacggc agccccaggg
    34621 tgatgttgac cgcgttggcg aagtccgcgt gcttgagcac cggcagcccc tggtcgtggt
    34681 acatcgccag caccgcatcg aagccacgca gcttgccggg caggaaggcg gtgtcggcgg
    34741 gcaacgggcc gaccagcgac aggccttcgc cgcgcaggcg ctcgagcgcg gggtcgatga
    34801 tgtcgagttc ctcccggccc aggtggccgt cctcgcccgc gtgcggattc aggccgagca
    34861 cggcgatggt cggcgaggcg atgccgaatt gcctgcgcaa tgcctggtcc acgatgcgca
    34921 aggtcgatcc cagcgcatcg gcggtgatcg cgtcggcgac ctcgcgcagc ggcaggtgga
    34981 ctgtctgcag ggccacgcgc atgcccgggt tggccaacat catcaccacc tcgcagccgg
    35041 ccagcgtggc cagcaggcca gtggtgccgg tgaacgggat gccgccctgg ttgatgacgc
    35101 ccttgtgcac cgggccggtg accaggccgt ggcatccgcc ggtgaggcaa gcttgcgcgg
    35161 cgcggcgcaa cgacgcgact acggattcgg cgttggccgg atcaggctgg ccgaaggcgg
    35221 ccgcggcggg atgcgcgatt tcgaccagtt ccaggctgcg cggcgaacgc ggcgactcgc
    35281 cgttgcgatg caatgcgatc ggcaggccca gcgcgtcgcc ggcgcgatgc agcgtcgccg
    35341 cgtcgccgtg gaccaccagt tgcgcctccc agtcctgctg ggcggcgcga acgatcaatt
    35401 ccggaccgat gcccgccggt tcgccgggga ccagcgcgag ccgcgggcgc atcggcggcg
    35461 tggctcagcc gccgctggcg ggcgcggtgg cggccgtttc ggccttgtcg gcacgcacgt
    35521 cgacgaaggc ctcgccgcgc atctcgcgca ggaagcggtt ccattcctct tccagcttgc
    35581 gccggccgat ggtttcgcgg agctgcgcgc gctggctttc atcggccgca gccacctggc
    35641 gactgccgat gcgctgcacg atcacccagc cggcctcggt cttgaacggt gccgaggtct
    35701 gcccgtcgga gagggacgcg acctgcatgc cgaaatcggt gccgtaggta tccgcaccga
    35761 accagcccat gtcgccgccg cggcgcttgg tcgccgcatc gtcggaggat tcgccggcca
    35821 gcttggcgaa ctctgcgccg cccgcaaggc gcgcggccag ggtctcggcc ttggccttgg
    35881 cggcggcatc gtcggtcttg tcgtccacct tgaccacgat ctggcgcgca gagaactggg
    35941 tcacctgctc gccgctgccg ccggcctggg tgcgggtttc caccagttgc agcaactgga
    36001 agccgctggg tccacggatc gggccgatga cctggccgtt ctgcatctgc tggatggtct
    36061 gcgcgaatgc cggcgggatc tcgttgaggc cgcgccagcc caggtcgccg ccttccagcg
    36121 cattcgggct gtccgaatac cgcacggcgg cggcggcgaa cgtcatctcg cccttggcga
    36181 tcagcgcctg cacgccttcg atcttcttct gggcgacggc caactgctcc ggcgtggcgc
    36241 catcgggtgc acccaccagg atgtgggcca ggtggaactg ctggcttgcg gccgtcgagg
    36301 cggtggccat ggccgcatcg acctcggcct cgctcacgtt gatgcggctt tgggcgaagc
    36361 tctggcggag tttctgggtg atgatctcgt cgcgcaggtt gttgcggaat tcggtgaagg
    36421 ccatgccgtc ctgggccaac cgctggcgca gttggtcgac gctcatgctg ttctgctggg
    36481 ccacgccctg caccgcgcgc tccagttcct catcgctggc attgatgccg gagtcggtgg
    36541 cgcgggcgat ctgcaggcgc atcagcacca ggcgctccag cacctgccgc tcgagcacgt
    36601 cggccggtgg aagctgggct tccttgcccg cgtactggct gcggatgttg gcgacggcgc
    36661 gatccagctc gctgcgcagg atcacgtcct cgttgacgac cgcggcgatg cggtcgaccg
    36721 gctggctgcc ggcataggac tgcgcctgca cgttgcccac cagcagcagg ctggcgaggg
    36781 cggcggcaag gaggggtcgg gaggcgcgct tgctgaaagc tggggtcatg gaagggtgtc
    36841 ggcagcggaa tcgggcgggg tgggctggcc cgtggcggtc tgcggcggca ccagatacag
    36901 gtcatcgcga ttgtagccga ggatggatcg gcgcaaggcg cgacgcgtgt cctggccggc
    36961 ggagccaagg cccttcaatt cgatctcgaa catcagtgca ttgctgaggt cgccctgccg
    37021 attgtgcacg tagcgccggc cgacaagccg aaccgcgaca caacagctgt cccactgcag
    37081 gccggccatc gtttccaggg tcttgctgtc gtgcagcgag tagtaatacc ggccgaccac
    37141 gctccagctg gcgctgaccg ggtacaggaa ggccacgtcg acctgctcca gcaaggaccg
    37201 gcggtagcgg tagccgaggt tgaacacacc ctcgtccggg agcaggtagc gtgcgcgcac
    37261 gctggccagg tcggtgcgct tgaacttcgg atcccactgg taggacgcgc cgatggtcca
    37321 gcggtcggtc ggcgcgtaat tggcgtccgc gacccaggcc gattggcctt tttcggtgat
    37381 cggctcgccc gggatgaaca cccgcgactc gttgaagtaa cggatctggc cgaggctggc
    37441 ggagaagcgt tcgcgaccgt cggactggcg gatcatgcgg gtgctgaccg ccaacgtgag
    37501 ctggttggcg tccgcctggc ggtcggcgcc ggagtagcgg ttgtcgcgga acagctggcc
    37561 ccagctgaag gtcagcggct gggtgtcgaa cagcggcatg tcggtctggt cggcgtacgg
    37621 cacgttcagg tagaacagcc gcggctcgat ggtctgcagg tactccttgc ccttcaccgt
    37681 ggtgtcgcgg tcgaagaaca ggccggcgtc caggctgaag atcgattgtc cgcgcgacgg
    37741 cgaactgccg cccagcgcct gcgccaggcc gcggtcgagc gaatagccgg tatagcgata
    37801 ggccgcggtc gggcgcagga accaggaatt gccttccagc ggcaggctga tccacggctt
    37861 caggtcgatg cgcgacccgg cgtcgtaggc ctcgtgctcg aaacgcacgg cttccgcgcc
    37921 gatgccgccc agcagcaggc cggccagccg ctgctcccag ttgaggtagg cgcgcggcag
    37981 gcggctgaag ggcagcgcct cttcggtcag ggtgaagtcg gccagctgcc agtggtcggc
    38041 ggtgacaccg gcgtcccagc cctggccgcg cccgtacacg ccgatcgcgc tgtaggcgct
    38101 ggtctgcgac agaccgtcca ggctgttgct gaagtcctcg tagtagcgcg aatcgctgat
    38161 ccagttcagg ttcgcccgcg cctgccagtg cgaggagatg ttctggaagg cgtcatagcg
    38221 gagcaggctg cggtcgcgat cgcgcaactt gtcgtccggc atccaggtgc cttcgaccac
    38281 gccttgcccg ccttccacca ggtagcggaa ctgcgcgccc aactggacgc cgcgttccgt
    38341 catcaggcgt gggttgaggg tggcgtcgta gttcggcgcc aggttcaggt agatcggctg
    38401 ccggtagtcg aagccattgc ggctggaatt cgagatcgac gggaacagca ggccggtgcg
    38461 acgccgctcg tcggtgggaa acatgaacca gggcatgtac agcacgggga ccttgcccac
    38521 ccgcagggtg gcgccgcgcg cgacggccat gccttcctcg gtgtcgatgt cgatctggcg
    38581 ggcgcgcagc tcccattgcc tcgcgtccgg cggacacgtg gaataggtcg cgccgcgcag
    38641 cgtgcccatc tcgccggcca gggtcacgct gtcggcaccg ccgttgccgc gtcgcgacag
    38701 cagttggtag tcgaggtcgt ggatgacgtg ggtttccgtg ccctggttgc cttcggcgcg
    38761 ggccgcgacc atgcgcaggc cggagccctg gtaacgaatg ctgccctcgg cggtgtagcg
    38821 gtcggcttcc ttgtcgaagg tgatctggtc ggcccccatg aactggtcgc cgcggcgcat
    38881 ggtcacgttg ccctggaaga ccgggttggc ctccgtgccg gagagcgcat cgccttcgat
    38941 gtcggtggac tgactggaat tgtcgatgtt gatgccctcg ggcgcggcct gcgcctccac
    39001 gaagggcttg accacgtcct ggaccgggca caagccccag ttgggcggat cgtcggcagc
    39061 gtgggcatgc aaggagaacg cgatgcacag cggcagggga agcaggcgca ggactgggcg
    39121 cacgggtgat ccggttggcg gaaaaacaga cggtaagctt gccggaacgg gcccccacgg
    39181 gcaaaccgac ggcttcgttc acgtgggcct tgcgtggatc ccgcagactg tacgccgaac
    39241 cgtggaggac gcgcgcgatg aatgaccacc acctgcccac caccgacgcc gaatggcgtg
    39301 agcggctgga cccgcagcag tacgcggtct gccgttgctc ggccaccgag ccgccgttca
    39361 ccggcaagta ctgggaccac aagggcagtg gtgtgtatgc ctgcgccgcc tgtgctgcgc
    39421 cgttgttcgc ctcggcggac aagtacgact ccggcagcgg ttggcccagc tacctgcggc
    39481 cggtctccgc ggaagccgtg actgagcacg aggacggcac ccacggcatg cgccgcaccg
    39541 aggtgcgctg cgcgcgctgc gattcgcacc tgggccatgt gttcccggac ggccctgcgc
    39601 cgaccggctt gcgctattgc atcaattccg cggcgctggc gttcgtcccc gatccggcgt
    39661 gagccggcga ccgggtcagg cgcacagcgc gccgacgtgc gccacgctgg attcggccag
    39721 cgccgccaag tcgtaaccgc cttccaggct ggagacgatg cgcccgccac aatggcggtt
    39781 ggccagcgcg accagtgcgt cggtgatcca cgcgtagtcg cccgcctgga gccgtagctg
    39841 ggccagcgga tcgcgccagt gtgcgtcgaa tcctgcagac accagcagca gctgcggacg
    39901 gaagtcgtcg accgcgggca gcagcacgtc gtcccaggcg cgacggaaca ggacgccttc
    39961 ggcctcggcc ggcagcggca tgttgacgat gttgccgacg ccggtttcat tgcgcgcgcc
    40021 ggtgtcgggg taaagcggcc actggtgcga gctggcgaac agcacgcgcg ggtcgcgttc
    40081 gaagatcgcc tgggtgccgt tgccgtggtg gacgtcgaaa tcgacgatcg ccacccgctc
    40141 cagcccgtgc ttgtcgaggg cgtgcagggc ggcgatcgcg atgttgttga acaggcagaa
    40201 acccatcgcc acgtcgcagg tggcgtgatg gccgggcggg cgaaccgcgc agaatgcgcg
    40261 ccgggtcttg ccggacagca cggcatcgac cgcggccacg gcggccccgg cggcgcggag
    40321 cgcagcttcg gccgaggttg gcgacaacac cgtgtccgga tccagcagga tccgctgcgc
    40381 cgggtgggtt tccagcaccg catcgagcag gtcgggcgcg tgcgcccgca gcaactggcc
    40441 gcgggtcgca cgcggggctt catgccactc cagtgccggc aactcgcggc gcagggcgtc
    40501 gaccaccgcg gtcaaacggg cggggcgctc cgcgtggtcc ggtccggtgt cgtgggccag
    40561 gcaggcggga tgggtgtaga cacgcatcgc gggatcgtct tgctgccgtc gactagctgc
    40621 gacgcgcgtg ttcccacagc acctcgccct ggccatcggc gcgcgccagc acgcgcgcca
    40681 gcacgaacag caggtcggag agccggttga ggtaacggat cgcctgcggt cgcacggcgt
    40741 cgtggcggga cagggtcacg ctgtcgcgtt ccgcacggcg caccacggtg cgggcgatgt
    40801 ggcagcgcgc ggcggcctcg ccaccaccgg gcaggatgaa atccttcagc gccggcaggt
    40861 cgtcgttgta atggtcgagg cgggcttcca gtgcttcgac gtcggcgtcg aagatcgccg
    40921 cgtggccggg gatgcacagc tcgccgccga ggtcgaacag ctggtgctga atgcgcacca
    40981 gcacttcgcg gacgtcatcc ggcagggtgg cggccagcag caggccgatg caggaattgg
    41041 cttcgtcgac ggtgccgtag gcgatgaccc gcgcggagtc cttcgccacc cggctgccgt
    41101 cgccgaggcc ggtcgtgccg tcgtcgccgg tcttcgtgta gatcttgctg aggcgattgc
    41161 ccatgggcga tcgggtacgg gatcagaccg tctgcgggcg cagcgccggc aggcgctggc
    41221 gcatcagctc gaagccaccg aacagcagcg ccgaatagaa cagcgtgccg agcaccgtcc
    41281 actggaagaa cgggacgccg gccgcatagc aggcgaggag tcccgccatg gacttgggat
    41341 acagcggatc cgagagccag gcgccgaaat tggtcaccac gaagaacagc atggagcccg
    41401 ccagcgaata gcccagcacg cgaccggcac cgaccttgcc acgcaggccg aagcccagca
    41461 gggtcgacaa tgcgatgcag gcgtacacca gcaggtagcc ggcgctggtg aagtagtccc
    41521 agtagatgcc gccattgagc aggcccagca ccaggtcgga ggcgaacatc gccagcagcg
    41581 gcaccagcag cgcccagtgg cgcttggcga aatacgcgcc gccgaacagg gccacggcct
    41641 cgatcggcga gaagttcggc gggtgcggga tcacccgggt cagcgcggcg atgaagatca
    41701 gcgcggccag catcagcggg ccggcggcag gcgttggggc gtggcgattc atcggtgtct
    41761 tggatgcgct aacaacggag ccggtagcat aacgcaatgc cgaacaccga ccgcgacgcc
    41821 accaccccgc acgacgtgct gatcatcggc ggcgggctgg tcggcgccag cctggcgatc
    41881 gccctcgatg gcctcgggct ggacgtgggc atggttgaag ccacgcaggc gggcgcactg
    41941 cccgcggtgt tcgaccagcg caacctcagc ttcgccgagg ccaccgtgaa tgcactggac
    42001 gcgcttggcg tgatgcggtt gctgcgcgca ccgagcggca gcatccgccg catccacgcc
    42061 agtcgtgccg gcgatttcgg ccgcatccgc ctggaagcct cggcgcatgg gcgcgaggaa
    42121 ttcggccgcg tcgtggtggc gcgcgatttc ggcgaggcac tcgaagcgcg gctcgcatcg
    42181 ttgcaacacc tcacccgtta tcgcccggcg cgcttcatcg ggctgggcga tacccgcgat
    42241 ggactgcgcc atgtccggat ctccgatgcc gatggcgagc gcgagatcgc cacgcgcctg
    42301 ctggtcgccg ccgacggaac ccgcagtggc gtgcgcgagg cgctcggcat ccgcgtacgc
    42361 gaacacgact atcggcagac cttgctggtg gcgcggttgc gcacgcagcg gccgccggat
    42421 ggcaccgcct acgaacggct gacgtcgagc ggcccgactg cgttgttgcc gcgtggcgac
    42481 gggcattacg gcctggtgca tggcgtcgcc agcgacgacg ccgaggccgt cgcggcgatg
    42541 gacgatgccg tgttccttgc ccgcgtgcag gtcgcgttcg gctggcgcgc ggggcgcttc
    42601 ctctccgttg gcccccgcag tgcgtatccc atcatcggct gcgtggccga tgcgctggtc
    42661 gcgacgcgcg cggtgctggt cggcaatgcc gcgcagagcc tgcatccact cggtgcgcag
    42721 ggcttcaacc tgggcctgcg cgatgcgctg accctggccg aatgcatcgc cgcgcagccg
    42781 ggcgatgcgg gcgatgaccc gttgttgcgg cactacgcag agcgccgcgc cgacgatcgc
    42841 acgcgcacgc tggcgttctc cgatggattg gcgaaattga cctcgaacga tgcaccgttg
    42901 ctgcggccgt tgcgcagcgc gggcctgctg gccggcgatg cgatgggctg gctgcagggc
    42961 tggctggtgg gcggcgcgat gggctatcgc ggtgacgtgc cggcgctgtg ccgcaggggc
    43021 gtcgcgtgag ccggcgtggc ccgctggacg tggtggttgc cggtggcggc gtggtcggcg
    43081 cggccacggc cttgatgctc gcgcgcgaag gcctgcaggt cgcgctggtg gagccgcggc
    43141 aaccgcccgc gtggcagcgc acgatccgcg acctgcgtgt gtatgccttt gcaccggata
    43201 acgcagcgct gttcgcggaa ctcggcgcgt ggccgggcgt gctggactcg cgcgtgcagc
    43261 cgtatcggcg gatgcaggtc tgggatgccg ccggtggcga tgccctggcg ttcgacgccg
    43321 acgcattggg gcagccgcaa ctcggctgga tcatcgagaa caatgccctg gtcgatgcgc
    43381 tgtggcgcct gctgcccacc gcgggcgtgc gcctgcactg cccggcatcg atcgaggcgc
    43441 tggaacagga cggcacccgc gtgcgcgtcg gcctggacga tggcctggtg ctggacgcga
    43501 ggctggccat cgccgcggac ggcggccagt ccgcgctgcg caccctggcc gggatcggca
    43561 ccacgagcca tgactaccgg cagcagggcg tggtcgcctt cgtcgccagc gagcagccgc
    43621 atcgcgacac ctgctggcag cgtttcctgc cgaccggacc ggtggcgttg ctgcccttca
    43681 acgacgatgg cgatgccgga ttgcgtggcc ggctcggatc gatcgtgtgg accttgccgg
    43741 acgtggaggc ccggtgcctg cgcgatgccg atccagccga attcgagctg gcattggcga
    43801 ccgccttcgg tggcgagctc ggcgcattcc acctgcaatc cgcgcgcgcc gcgttcccgc
    43861 tgcgacggca actggcggac acgcatgtcg ccgggcgcgt cttgttgctg ggcgatgcgg
    43921 cgcacgtggt gcatccgctc gccgggcagg gcgtgaacct gggcctgcgc gatg
//
LOCUS       DAOOCX010000004        40863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_692_length_40863_cov_8.533131, whole genome shotgun sequence.
ACCESSION   DAOOCX010000004 DAOOCX010000000
VERSION     DAOOCX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 40863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40863
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_692_length_40863_cov_8.533131"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2042)
                     /locus_tag="PK399_00730"
     CDS             complement(<1..2042)
                     /locus_tag="PK399_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HPW11686.1"
                     /translation="MSATRKLWLGLAVLLTLSFGVLLWAGTEIFRTAPPMPEKVLSES
                     GQVIYTRADMEQGRQVWQSMGGMQLGSIWGHGGYVAPDWSADWLHRESVSLLDLWARA
                     DGGMATYAQLPEEQQAALRARLKQVMRTNTYDAATGTITLPDDRALALSNVAAHYESL
                     FGNDPATATLREAYAMKNDTVPDASHRRALTAFFWWTAWAAGTERPLEPGDAMVPSKT
                     GVVGKQVTYTNNWPSEPLIDNTPAPAMWVWSSFSVLFLIAGIGLLGWWHARQAHEEPL
                     QLPASDPLAAIRITPSMRATAKYFWVVLALFVVQILLGAITAHYQVEGQQAYGFALSD
                     VLPYSITRTWHTQLAVLWIAVAWLGTGLYIAPAMSGHEPKFQRLGVNFLWTCLLLIVV
                     GSFAGQWLAVMQKLGLEHNFWWGHQGWEYADMGRFWQWFLFVGLLLWLTLVGRALWPV
                     LRGPATGTRSIVGLLFLSTVCIGLFFASALMWGEHTHISEVEYWRWWLVHLWVEGFFE
                     VFATAVMALIFTRLGLIKDTVASVAVLFATIVFMSGGVLGTLHHLYFVGTPTAVVALG
                     ASFSALEVVPLAYIGFEAYHTYKLGKATPWMARYRWPIMFFIAVSFWNLVGAGLFGFL
                     INPPLSLYFMQGLNLTPLHGHTALFGVYGMLGIGLMLFCLRGLKPDVQWNEGWLRGA"
     gene            complement(2126..2878)
                     /locus_tag="PK399_00735"
     CDS             complement(2126..2878)
                     /locus_tag="PK399_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW11687.1"
                     /translation="MSSVPFPRFNPRVLADDGSDLQFCTTCAFSHACLSQGMDKAALM
                     DLHVLVEHVGPYHAGDVLFREGDPFEAIAAVRAGTVKTCVTDRDGHEHVLGFHFPGEV
                     IGLNAIDGHRYPCDAVALDTVMLCRFSFPKISVLAARVPGLQRELFRLLSRDIGRAAL
                     LAGDWSADQRVAAFLVGMSRRLAARGFSASHFQLTMARTDIANYLRLAPETVSRVLRR
                     MQDDGLLAVERRAVSLLDRERIEALAAPILRE"
     gene            complement(2898..4301)
                     /gene="hemN"
                     /locus_tag="PK399_00740"
     CDS             complement(2898..4301)
                     /gene="hemN"
                     /locus_tag="PK399_00740"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HPW11688.1"
                     /translation="MPSQQIAFDAGLLRRYDRPGPRYTSYPTAPQFSADFGEAALREV
                     ARASNEDPIPRPLSLYVHVPFCFSPCFYCGCNRIITRDLARGRAYLDRLGHEIALVAP
                     LFDRDREVIQLHFGGGTPNFLDAAQLCGVVETLRGAFRFADSDDRDISVELDPRFCNP
                     ADMPVLAAAGFNRTSLGVQDFDPKVQAAVNRIQSVEETRAIVDACRGAGMRSVNIDLI
                     YGLPGQSLDGFRKTLDTVVAMRPDRLAVYSYAHLPHIFKPQTQIDDALLPDPDTKLAL
                     LQAAVEHLTAAGYVYIGMDHFALPDDELAVAQARGGLHRNFMGYTTHADSDLVGLGVS
                     AISHIGGSFSQNPRDLPSWETALDAGRLPVFRGMRMDEDDEIRADLIQSLMCQGEVRV
                     RAFQERHGIGFAEYFAADLQRLWPLADDGLVEVGPDAIRATPRGRLLLRNIAMCFDRY
                     LDQTSVIDARPRFSRAI"
     gene            complement(4366..5019)
                     /locus_tag="PK399_00745"
     CDS             complement(4366..5019)
                     /locus_tag="PK399_00745"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HPW11689.1"
                     /translation="MHPDFWHQRWADNQIGFHQDAPTPLLLKHWGSLGVAPGAKVFVP
                     LAGKSRDMAWLASQGHRVLGVELSPLAVEQFFAEHGLQPDTRESRYGRHYEAGGIELV
                     VGDAFGLDAELLRDCAAAFDRAALIALPADVRLRYARDLYAALPAGCRGLLVTLEYPQ
                     AEREGPPFSVPQDEVQALYEADWRVELRERRPVPPDHPGFVAGVSRLDTAVYALQRR"
     gene            complement(5073..7319)
                     /gene="parC"
                     /locus_tag="PK399_00750"
     CDS             complement(5073..7319)
                     /gene="parC"
                     /locus_tag="PK399_00750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HPW11690.1"
                     /translation="MTDTPVRSSFHGFEQIPLSEFAERAYLDYSMYVVLDRALPFIGD
                     GLKPVQRRIVFAMSELGLNAGAKPKKSARTVGDVIGKYHPHGDSACYEALVLMAQPFS
                     YRYPLIEGQGNFGSSEDPKSFAAMRYTESKLSPIAEVLLGELGQGTVDWDPNFDGTLE
                     EPTWLPARLPHLLLNGTTGIAVGMATDVPPHNLNEIVSACVRLLDDPDATVRDLCEHV
                     RGPDYPTAAEIITPAADLQAMYETGSGSVRARGVFVKEQANIVITELPYQVSPSKIIE
                     QIANQMRAKKLPWLEDIRDESDHANPTRIVLVPRSNRVDAEQLMGHLFATTDLEKSYR
                     VNMNVIGLDGRPQVKHLKMLLTEWLEFRQTTVTRRLTHRLEKVERRLHLLEGLLVAFL
                     NLDEVIRIIRSEDEPKAALIARFGLSDDQADYILDTKLRQLARLEEMKIRGEQDELDA
                     ERDRLMTLLGSKAKLKKLIKDELLADAKKFGDARRSPLVAREAAHALDETELVASEPM
                     TVVLSEKGWVRAAKGHDIDAAALSYRDGDGLLAAVRARSNLQVAFLDGAGRSYSTAVH
                     TLPSARGNGEPLTGRFTPAAGAGFQAMASGENDMRLVIASSHGYGFVTRFENLTGRNK
                     AGKALLSLSEGGRVLQPAAVVDMATDRVVAVTSAGHVLAFALADLPELDKGKGNKLIE
                     IPKAKRGTEHVVAIAVVPAAGKLQVKSGARTMTLSFKELDDYLGARGSRGGLLPRGWQ
                     KVDGLAIE"
     gene            complement(7382..7825)
                     /gene="rnk"
                     /locus_tag="PK399_00755"
     CDS             complement(7382..7825)
                     /gene="rnk"
                     /locus_tag="PK399_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPW11691.1"
                     /translation="MHARPSTAAASRPPLLLSRLDVERIEALLEQPAFRALDTAALQG
                     ELGRAELAEPAAIPADVITMNSIARVRVDDPMQGAHEHELTLVYPRQADGSADRVSIL
                     APVGSALLGLRVGDSIDWPLPGGRNARLQVLAVRYQPEAAGDLHR"
     gene            8005..8520
                     /locus_tag="PK399_00760"
     CDS             8005..8520
                     /locus_tag="PK399_00760"
                     /inference="COORDINATES: protein motif:HMM:NF020909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HPW11692.1"
                     /translation="MSNPRIQQLQQLFVSRLDTLSHILQVGQAHLGDRDLVNERLAPD
                     MLPLGAQVAFACNQPRGFSQWCAGQPIENIDPTTVASLEDAHAQIADTRARVLAISAS
                     DAKLDEVKRIGMGPGRYCELPGDLYVSEYLLPNLYFHITTAYAILRMLGAPVGKADYM
                     RYLGAHLRHDG"
     gene            8524..8892
                     /locus_tag="PK399_00765"
     CDS             8524..8892
                     /locus_tag="PK399_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW11693.1"
                     /translation="MDNRGIATEFLRLCARGDVREAYARFVSDDFVHHNPWFAGDRES
                     LLVAMEQSAAAEPNKSLEVKQVIDGGDRVAVHSHLTRADAGLQYAVVHIARIEHGRIV
                     ELWDLAQELPGESPNANGMF"
     gene            8979..9476
                     /locus_tag="PK399_00770"
     CDS             8979..9476
                     /locus_tag="PK399_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11694.1"
                     /translation="MKIRHVFVTRDVIQARDALLALREQGVGVDDISLVARSDIELDR
                     IPDELKEADTDFVPAAMRGMGIGGATGLLAGLAAVVFAPVGITLAGAAAIGALGALVG
                     GWSSALMGSALPDPVRQQFDDEISAGRILLLVDAEPDVQPRLQLALAAVGARRLDYEA
                     PSAMT"
     gene            9585..>10330
                     /locus_tag="PK399_00775"
     CDS             9585..>10330
                     /locus_tag="PK399_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11695.1"
                     /translation="MKARHLAVACGVAFAAATTAASANDAPAGGGVLRYVGEARDPGG
                     RLLYRETHYLDSRAPAPRRVVLYQCADGRPFARKTVDGAGPAPAFAFLDARDGYQEGV
                     RTQGGTREVFWQEGKASPLKRAAVAKTGATVFDAGFDAYVREHFPALQQGKAVRARFL
                     IPSRQSAIGVSVKPVAGAPAGYLDLKTRLDAWYGFALPELKLRYRVADRRLVRFEGIG
                     TIRDGKGGHGKLRIDFAQPAEAIEAGELQRA"
     assembly_gap    10331..10430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10546..11124
                     /locus_tag="PK399_00780"
     CDS             10546..11124
                     /locus_tag="PK399_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW11696.1"
                     /translation="MSAIPLQQSLDGSADPLAALLAQVAEGRRDAFESLYRQTSATML
                     GICLRVLRDREEAEDVLQEVYVAVWSKAAQFDQQRARAMTWLGTIARNRAIDRVRARP
                     SPALHEPIEDHELADDEAPTPAAGIDAALERAQLDDCMDQLEPKRRSLIRTAFFENVT
                     YDVLALRAEAPLGSVKSWIRRGLQQLKACLER"
     gene            11121..11891
                     /locus_tag="PK399_00785"
     CDS             11121..11891
                     /locus_tag="PK399_00785"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HPW11697.1"
                     /translation="MNDIAPALPPIDDDDLLAGEYALGVLDPDARRATQRRIDADRDF
                     AARVARWDAHFSPWLLRVDAVEPGAQVWPRIRTSLGWSAVEGHRKGLWDNAAFWRGTT
                     ALAVAASVFAVAIGVWRTPEPAAPVPPPVVVVQPPPATDEAAALPVTVLATDDGNTGW
                     IARVAPDRSKVLMVPKPRPLDAGGKVNELWIIASGEAPKSLGFVSNDKAHSIDIPATL
                     RDAVAAGAVFAVTLEPEQGLPHAAPSGPVVAKGQILSI"
     gene            11975..12934
                     /locus_tag="PK399_00790"
     CDS             11975..12934
                     /locus_tag="PK399_00790"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HPW11698.1"
                     /translation="MPPKPDSTNAPRGSALLRWIDTSGQSGSPDPANDDRIDWLRALP
                     FVGMHLACLGVLWVGVSATALWVALGLYALRMFAITGFYHRYFSHRTFRTSRAVQFAF
                     ALLGAASVQRGPLWWAAHHRHHHVHADTAADPHTPRKGFWRSHAGWFLTRGGFRTDTS
                     RIGDLLKFPELRWLDRFDTAVPVALAVLLFLAGMALERWAPGLGTNGPQLLVWGFFVS
                     TVVLFHATVTINSLAHRWGRQRFDTRDDSRNNLWLALITFGEGWHNNHHFFPGSARQG
                     FRWWEIDLTWYGLRTLQALGLVRDLKPVPAWVLAKADANKAGR"
     gene            12936..14234
                     /locus_tag="PK399_00795"
     CDS             12936..14234
                     /locus_tag="PK399_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW11699.1"
                     /translation="MRIAIVGSGISGLSAAWLLSRQHDVVLFEANDYLGGHTDTHRVE
                     VDGRTLDVDTGFIVHNPGNYPLLTRLFDELGVATQSTTMGFSVQDARTGLEYNATSLD
                     GLFCQRRNLASPRFWGMLRDLARFYREAPALLDDDHPGPSLGEYLDANGYGAAFRDQH
                     LVPMASALWSSPEQSVLAFPAKYLVQFMANHHMLQLTGRPEWRVVRGGSRRYVEAMVS
                     HWDVQVRLSSPVRGIARDAHGVSVRCDAGSERFDQVVLACHSDQALHLLDDASDAERD
                     ILGAIRYQANDTVLHTDARLLPRNRKAWAAWNAHLPADPAQACTVSYCMNLLQGLPGD
                     TPLVVTLNRTHDIDPARILQRRQYAHPVHTHAAVAAQARRHEIQGVQRTWFAGAYWGW
                     GFHEDGMRSGVEVAQGMGVAWSNDQVRRGAVDTQPLEAAA"
     gene            14231..15001
                     /locus_tag="PK399_00800"
     CDS             14231..15001
                     /locus_tag="PK399_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HPW11700.1"
                     /translation="MTVLASAIYEGRVRHRRHAPRAHAFSYRMAQLYLDLDELDAAFR
                     GRWLWSVGRRNLAAFHRRDFLGDPARPLADAVRDRVEAATGERPLGAVRLLTHLRYAG
                     LSFNPVSFYYCFDAGGTLHSIVAEITNTPWKERHAYVLPVATAERRGRALHWRFDKAF
                     HVSPFLPMQRRYGWTFTEPGDGLQVHMDVCDGSEREFDATLQLERRPLTGANLARVLW
                     RYPLMTAQVVVGIHWQALRLWLKRVPVHDHPTRMESPR"
     gene            14998..16266
                     /locus_tag="PK399_00805"
     CDS             14998..16266
                     /locus_tag="PK399_00805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HPW11701.1"
                     /translation="MNARADASPATGFSALDRLLRNRVLDQLRALRGRAIVLADPLGC
                     IRTGDAEDAAAPLRIDVHGPGFYRALAANGSVGAAESYMDGEWDCADLVGLVQLLVRN
                     RDLLDAMERGPARIGGWAMRGWNALRRNTRDGARRNIAAHYDLGNDFFRLFLSEDLMY
                     SSALYAGDDDTLEAASTRKLDRICDKLRLGPDDHVVEIGTGWGGFAVHAARTRGCRVT
                     TTTISREQHALASQRVADAGLQDRVTVLLSDYRDLQGQYTKLVSIEMIEAIGAQYLDT
                     YFATLGRLLKPDGLALVQAITIEDHRYEQALRSVDFIKRHIFPGSFIPSVAAMLAAKT
                     RSSDLALVHLEDFGPSYARTLHDWRERFLARREDVRAQGFDERFLRMWTFYLAYCEGG
                     FRERSIGVAHVLMAKPGYRGGSYLPGLLEA"
     gene            16266..16802
                     /locus_tag="PK399_00810"
     CDS             16266..16802
                     /locus_tag="PK399_00810"
                     /inference="COORDINATES: protein motif:HMM:NF022537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HPW11702.1"
                     /translation="MRFWLNLLGYQAAWFIAVGFAARGLAWPGMLACLGFAAISWWTS
                     PLRRSDLKLIGAALACGLLLDGVLAATGWLRYAAPLPALPGPAWIATLWVAFAMTLQH
                     SLQWVIARPAVAIAFGVIGGPLAYWGASRAFDAVVFIMPLQATLALALGWGIAMAAFV
                     LIVHRRHASITHQPGVSA"
     gene            16799..17581
                     /locus_tag="PK399_00815"
     CDS             16799..17581
                     /locus_tag="PK399_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HPW11703.1"
                     /translation="MTPWQQLLLVWLVAVVAQSLGWLWQRKRSNAGIVDVVWSFGVGG
                     AAVLVAATGSGALLPRVLLAMLGGAWGLRLGLHLWHRVRAEPEDGRYAQLRQRWGNDQ
                     RKWFAMFQFQALLIAFFALPFLAVAANPVADWNAWFMAGIATWLACVAGEAVADAQLA
                     KFRTNPANKGKTCRDGLWRYSRHPNYFFEWLHWLAYVLLAVGSPIAWLAWSGPVVMYV
                     FLRWISGIPFTEAQALRTRGEDYARYQRSTPMLFPWFPKEDR"
     gene            17599..18660
                     /locus_tag="PK399_00820"
     CDS             17599..18660
                     /locus_tag="PK399_00820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HPW11704.1"
                     /translation="MELASDRPADGVLGLAERGLVPDALLRAGIRRMCAQRLRDEHAG
                     DPDAAARRNAALIAELRESPVAIHTDAANRQHYELPPGFFTRCLGPRLKYSGCYYPTG
                     NETLAQAEEAMLALYGERAELADGQDILELGCGWGSLTLWMAQHYPNARITAVSNSAP
                     QRAFIEAQCRERGFANVRVITCDVNRLELPASNFDRCVSVEMFEHMRNYATLLGRIGG
                     WLRPGGKLFVHIFCHRTLLYPFETAGDDNWMGRHFFTGGLMPAADTLLWFQDALRIED
                     RWLVDGTHYERTANHWLENQDARHDEVMAILREAYGDAARLWFQRWRMFWMACAELFG
                     YDHGREWMVAHYRFSKEVA"
     gene            18744..19199
                     /locus_tag="PK399_00825"
                     /pseudo
     CDS             18744..19199
                     /locus_tag="PK399_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357385.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
     gene            19233..20606
                     /locus_tag="PK399_00830"
     CDS             19233..20606
                     /locus_tag="PK399_00830"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPW11705.1"
                     /translation="MTQGLFWFRRDLRLADHPALARACQSHDRLLLVYVDDDADGPFD
                     ASRAWLRLSLGKLGDDIRARGGELHLLAGDPEILIPALAVAAGATEVHISALHEPKAA
                     ARDARIAATLAGRAIAMRRAGGRLLTDAESVLTQSATPFRVFTPFHKAARPTWRHRPR
                     EAPTALACSPLPSSLEGARTTLDAPRPRWDKGFWATWTPGEAAAHARLDDLLPRLHAY
                     PDARDVPSVEGTSRLSPHLHFGEIHPSRIFDVVQRQGGIGADKYIAELGWREFAYYVL
                     HHWPASLHDNFNPKFDAMPWRDDPAGLEAWQRGLTGVPLVDAGMRQLWHEGWMHNRVR
                     MVVASWLTKHMGIDWRQGAAWFMHTLVDADLASNTLGWQWVAGTGVDAAPYFRIFNPV
                     TQSRRFDPQGAYLRRWLPELRGLGDDAIHAPWERGLRVPGYPVKPIVDLGAGRDAALA
                     RLAALAK"
     gene            complement(20676..21509)
                     /locus_tag="PK399_00835"
     CDS             complement(20676..21509)
                     /locus_tag="PK399_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11706.1"
                     /translation="MRGVNKQVRNTLALAVVAVMLAACGKAGDAASTATPASGMQAMV
                     DQAAQDAADTGALANGDAPLKEGQAVQGTIQADVGNGTQSFRSLSTKVADDIGEQLDE
                     KLGRSEGQDAIADANKKLEKLGTGMKVDAGDVREIVGGMAGKTFHDSQVMHVDIIKSL
                     QVTLKGTAGDGRTLDLGITFDDQTLAMTGSSLNYRPKANAMFDFYEGKGGEQVTVTID
                     RFERNADGTYAIAGSFTAKDVPASSLAKKLPAKTLPSASGTFSFDALPLKEMPKFGTG
                     G"
     gene            complement(21708..22226)
                     /locus_tag="PK399_00840"
     CDS             complement(21708..22226)
                     /locus_tag="PK399_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11707.1"
                     /translation="MPLRILLAILIAMLASACTAADADIPAAIAPVVADDTTAVAMPS
                     PARLHGNWRLVAADDPHDAALMAFSIQADAGDTTGSGDYVLYQPFCDVIAGLPVTGDA
                     DCELIGLGATFDRVWIEGDSIVLVSRPTADGMEHRLELQRRGDSLVGHYIIRANDIRR
                     AVIAMRPRAGTP"
     gene            22320..22901
                     /locus_tag="PK399_00845"
     CDS             22320..22901
                     /locus_tag="PK399_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HPW11708.1"
                     /translation="MQAFPAEVIDRMHEDAEPQPGTLAATAAELAPLLLDDMRRRARR
                     ERRRVNAGETMRTTALVNEAFLRLQRGGAWEDETHFLHAAAMAMRQVLVDHARRRLAG
                     KRGGGRVDSLEDHPDIEPFWVSDERLVELDDALQELSRLNPRLQQVVECRFFAGYTDD
                     ETARVLGVAERTVRRDWIKARAWLFQALEPVPD"
     gene            complement(22852..25305)
                     /locus_tag="PK399_00850"
     CDS             complement(22852..25305)
                     /locus_tag="PK399_00850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPW11709.1"
                     /translation="MTSTPEWQAIETLFDAAWDMPAPQREAWLRSTGRPAAVIDEVLR
                     LLAAADASGDFLERAAHADALSPATLAAGDSAGAWRIVGLLGRGGMGEVHEVERADGQ
                     YGQRAALKRIARVDAGDWARFDNERRIVALLDHPGIARMIDGGLLDDGQPYMVMEYVD
                     GMPIHQWCEQRNASPRERIALVLQACEAVAHAHARLVVHRDIKPSNLLVDQDGRVRLI
                     DFGVASLSGQGEEADGERAPLSLGYAAPEQLAGGDVGVAADIHAMAAVLYRLLCGHAP
                     HARDEPPTALLAMRAAQVNPPRLRDVAAAVSIPTERGLLLDLDAVLAQALARDPAQRY
                     RSMDAFADDLARALRGAPVAARDGEPRYRFWRFLHTHRWAATGASVTVLALAVGLGAA
                     LFQAREAGRQRDEALREKARLEAVQQAVFHMFRSAGEMQGSDATAGDVLDHAAQRVVD
                     SFARDPAEGAPVLHTLGELYFLLNDYPAAEPLLQRLADADTRIVDPSLVAVGRYDLAQ
                     VALRGGDAKRAAALLAQARAFWNTDPVRWQSRLIDSQMLEARLRQQAGDADGAIALLQ
                     RGLAQRIALDGVHGRETGVFQNNLGVALFGLGRLDESREAFRAARDVWRVAGLGESTD
                     ALNTLNNWGSLELSAGKPEVAAPLLEQAVTLRRTLYGPSAATAALLNNYGKLLLQTGR
                     AGEALPVLAEAAAMGAQFAGTGSMHHVAALSGVAEAQLALGDAKRAEVTAREALAAAD
                     QHLGAGHPGGAAPRLALARIHLARNEQAPVAALLDTVDAMAKAAGPAGQRIATQSASL
                     RAQLSPAPAPAPGTTTRAP"
     gene            complement(25454..25540)
                     /locus_tag="PK399_00855"
     tRNA            complement(25454..25540)
                     /locus_tag="PK399_00855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25504..25506),aa:Leu,seq:cag)
     gene            25794..28136
                     /gene="rnr"
                     /locus_tag="PK399_00860"
     CDS             25794..28136
                     /gene="rnr"
                     /locus_tag="PK399_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPW11710.1"
                     /translation="MRAFGGAGAAKPAGRADPKPATKPVMSTRPGRERDPHAAREAQR
                     YEQPIASREMILQILAASDGPLDADALAAKLALTAPDRFAALEKRLNAMLRDGQLLQN
                     RRGGFAPAARMDLVPGVVIANENGFGFLRPDAGGGDDLFLPPYEMRKVMHGDRVLASV
                     TGMDRRGRREGTIVEVLERRLTRLIGRYTEEAGIGYVVPDDKRVQRNVMIPANGRNDA
                     RNGQLVVAEITTPADAHRPPIGNVLAVLGDKLTASQAVEAAIHGHDIPHEFPPEVLAQ
                     ATAVPLQVQAADIAGRVDLRNVPLVTIDGEDAKDFDDAVWCEPNKDGFRLIVGIADVS
                     HYVRPGTPLDDEAQLRATSVYFPGFVVPMLPETLSNGICSLMPKVDRLCFVADMQVGR
                     DGIVTGARFYEAVMHSHARLTYTDVWNAVGEGIPEDDHAAALAKIGGLLPQIRQLHQL
                     YKVLDKARQKRGAIEFESSEVRFVLDAQGAVSQAGMLQRNDAHKLIEECMIAANVQAA
                     LYLLQSGVPAPYRDHDKPPESKYADLEEFLKEFMLRMPPWAKVKPKDFRAMLEKIRER
                     PDATLLESVILRSQSLAVYAPENIGHFGLALEAYAHFTSPIRRYPDLLVHRAIKHALA
                     GGKPETYTYSPHEMAALSLQCSERSRRADEAQREVDERYRAAWMEGHIGKEFDGTISG
                     VTSFGLFVELDDSKVNGLVHVTQLPRDFYHFDAVRKTLAGERRGHEYRLGDRVRIVVL
                     KASVEDRKIDFRLVGDEGAAAAPMPMPERGKPKKRTKQKY"
     gene            28268..29014
                     /gene="rlmB"
                     /locus_tag="PK399_00865"
     CDS             28268..29014
                     /gene="rlmB"
                     /locus_tag="PK399_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPW11711.1"
                     /translation="MSKQAQWIVGLNAVASALEHDAEHVREVLLEAGGKNPRIAEIEQ
                     EARRKEIDVRRVAQNALDGVAGGVRHQGAAARYAAAKTWDEHELVGLVEAAGGKALVL
                     VLDGVQDPHNLGACLRSAAAAGATAVVFPKDKAVQVNATVRKTSAGAADTLPIFSVTN
                     LSRTLRDLQKAGVWIYGFAGEATASFYALDLKGNVALVLGSEGDGLRRLTRENCDQLV
                     KIPMPGEFESLNVSVATGIALFEAVRQRQP"
     gene            29043..29309
                     /locus_tag="PK399_00870"
     CDS             29043..29309
                     /locus_tag="PK399_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11712.1"
                     /translation="MALELAWYDWVGLLGTVTILLGFFLLQAGRLAGTGLVYQLLNLF
                     GAAGILVSLMGKFNLSVFLLEAAWMAVSLYGIVRSIKAKRTQAR"
     gene            complement(29437..30069)
                     /gene="rnt"
                     /locus_tag="PK399_00875"
     CDS             complement(29437..30069)
                     /gene="rnt"
                     /locus_tag="PK399_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011259117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HPW11713.1"
                     /translation="MTDADAIPTDAPLFKPMVERFRGYLPVVVDVETGGFDWNRHALL
                     EIAAVPIELDEDGRFVPGEVASAHIQPAPGTSIDPKSLEVTGIKVDHPLRGAVPEREG
                     LDKVFMPVRAAAKRHGCQRAILVGHNAHFDLNFLNAAIARVEHKRNPFHPFSVFDTVS
                     LAGMAYGQTVLARAVHAAGLEWDQREAHSAVYDAERTAQLFCIIANAWPR"
     gene            30202..32571
                     /locus_tag="PK399_00880"
     CDS             30202..32571
                     /locus_tag="PK399_00880"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPW11714.1"
                     /translation="MPVLAASTDPVLQVNTFIGTQEEGNTFPGASAPFGMIQVSPITE
                     HYAGYRYDDTRIRGFGHSFVSGAGCWEQGGLVATLPVTGRIGPGGAFDTSDAKAKPFD
                     YKTYAAAYTHAGEVGQAGYYKVRLTDYNGIEVETTALTRAAAERYTFAVGEKVGHVLV
                     NVGQANEKHEVVGSVIDVVGDRAVEGKLVTRSFCGGKQYTTWFRIEFDRPFQAFGTWD
                     REGGVPGGRHSVGTQWDVANGAWLSFDLAGSRSVTATSAISHVDADGARRNLDAEGKH
                     AGKLLAFDAMRAQAQAAWRKELGVVRIDGAPDDDRVVFDTALYHAFLQPMTGSDADGR
                     YRGYDEAIHVAEGWTYHEYFSLWDTYRTQNQLLAILKPARARDIARSMLAIHEQMGWL
                     PRWGYANFDTNIMTGDPVTPWLVDLWRFGALQGREREALQALWQNANGRPPAMSRAQG
                     RAGNDTYLAQGFVPYDRAYPSKGMDVDQHHGGSATLEYALGDCALSQMAGALGETAIA
                     ATLRERGRNWRKAWDADVVDKELAYRGFPRPRIEGGAFYVPAHGGYDPRSNHGFHEGT
                     AWQYQWLVQQDVPGLVAAMGGIEQARNRLDAFFAQDELAKDVRGGARTHWVVGPYSYY
                     NQFRYNPNNEPDLHAPWMYTLLGQPWKTTTVVRAAQALFTNAPNGVTGNDDLGTMSAW
                     YVFSAIGLYPAVPGTGQLLLHAPRFPRVDLALGEGHVLTIRAAGANAGAAQAIAVVGI
                     DGKPHAQAWVDWATLRDGGSIDFTLAARADPNGWGTRPQDLPPSPCAAP"
     gene            32589..33584
                     /locus_tag="PK399_00885"
     CDS             32589..33584
                     /locus_tag="PK399_00885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPW11715.1"
                     /translation="MRPILCFGEALIDFLARPGDTPDAPRAFLQFAGGAPANVAVSVA
                     RLGGNAQFIGMLGRDMFGDFLLDSLRDAGVGTDGIVRTSDAKTALAFVALDADGERSF
                     SFHRPPAADLLFRDEHFIPAMFEGAAALHACSNSLTEPAIAEATYAGLRRARTAGALA
                     SVDLNLRQVLWAAEVDPTPWLWRLLDEAVLVKLARNELDYLAEPLGSEDAVFDRLFAG
                     HARLVLVTDGAADMHWRTRAARGTVAGFRVRSIDTTAAGDAFVGGLLFALVQQGVDAP
                     RFDAFINDEAAIRDAMRFGAACGALAVTRHGAFAAMPTHADVQRLLQDEDTHGPA"
     gene            33571..34803
                     /locus_tag="PK399_00890"
     CDS             33571..34803
                     /locus_tag="PK399_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPW11716.1"
                     /translation="MDQPDFRSPEFLRAHIAQTMAFYHPRCIDPAGGFFHYFKDDGTV
                     YDARHRHLVSSTRFVFNYAMAWREFGNTEYREAMLHGLEYLRRVHLDTETGGYAWTIR
                     DGVAEDRSNHCYGLAFVLLAYSHARMAGVADAADWMEEIWMLLEARFWEAEHGLYRDE
                     ADADWNFSDYRGQNANMHMCEAMLAAYEASGDKRWLDRALLLAGNMTRRQAAQAGGLV
                     WEHYDRDWNVDWNYHLDNPKHLFRPWGFQPGHQTEWAKLLLILDRHVATDWLAPMARH
                     LFDTASARSWDTERGGMYYGFAPESRRQPGMDGAPIGGGSFVCDDDKYFWVQAESLAA
                     AALLAARFDDQEYWDWYERLWAYAWAHMVDHQHGAWFRILDADNRKYDDNKSPAGKTD
                     YHTMGAAYEVMNLLRPVQ"
     gene            34839..36221
                     /locus_tag="PK399_00895"
     CDS             34839..36221
                     /locus_tag="PK399_00895"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HPW11717.1"
                     /translation="MLSMLAMAPAMAQQATEYVAPPAPTYAQPMDDAESARMAARVRE
                     ETRHAWQGYMRYAKGHDALKPLSRKPHDWYPQSLLMTPVDALDTLLLMGLDTEAAEAR
                     ELIATQLSFDHDMPVQNFEVVIRLLGGLLSAYQLTGDERLLALADDLGTRLLPVFESP
                     TGLPYTHVNLRTGAVKGKVSNPAETGTLLLELGTLSKLTGKPIYYDKAKRALVATFER
                     RSTVGLVGSGIDVETGEWTDRDSHVGGGIDSYYEYLLKCWKLFGDQDCKRMWEESVGP
                     MHRYLADEVRDGELWYGHADMDSGVRTTSVYGALDAFLPAVLALGGDLDRAKRLQQSG
                     QAMWRLHGIEPEAIDYRKIEVLSGGYPLRPEIIESAYYLHHFTRDPRWRAMGREFFDD
                     FVRHTRTEAGYAAIKDVRTMEQRDSMESFVLAETFKYWWLLFSPPSALDFDGVVFNTE
                     AHPLRKTWKD"
     gene            36704..36988
                     /locus_tag="PK399_00900"
     CDS             36704..36988
                     /locus_tag="PK399_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11718.1"
                     /translation="MHPQGIGAAGEATGSCHLVHVGDRTILLDCGLVQGGRSADKRNR
                     EPFPFDASTIDAAVLGHAHIDRKEFATTAEVMQPGERPNLDGSARVRVSR"
     gene            36985..38535
                     /locus_tag="PK399_00905"
     CDS             36985..38535
                     /locus_tag="PK399_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HPW11719.1"
                     /translation="MSAAKDSVAGDVVVAAHDRLVATRTGIDTRGEPVVYLRNDAAIA
                     RSEGFAALARIRVRANGHAIDATLFTVSGPRCMLAPGQVGLSEAAFSALDVEEGAALT
                     LSHAPAVESLGFLRAKVYGQRLDGQAFNAIIRDIVGGRYMGVHLAAFITACSGARLSP
                     DEIVDLTGAMVDVGQRLQWPGKCIVDKHCVGGLPGNRTTPIVVAIVAAHGLTMPKTSS
                     RAITSPAGSADTMETLAPVDLDLATMRRVVEREGACLAWGGSVSLSPADDILIRVERA
                     LDIDSEGQLVASVMSKKIAAGSTDILIDIPVGPTAKVRSRAAADALEAQFQYVAQAFG
                     CRIQVLQTDGSQPIGRGVGPALEARDVLAVLKCSADAPADLRERAVLLAGTLLESVGA
                     VAAGQGQFEASATLDDGRAWRKFQAICEAQGGMRVPPVARHRQDVFAPRSGVVRRIDN
                     RLIARAAKLAGAPGDKAAGLFLHVKLGDRVREGDPLLTLHAESPGELAYALDYARTQG
                     QAFEIGED"
     gene            38535..39443
                     /locus_tag="PK399_00910"
     CDS             38535..39443
                     /locus_tag="PK399_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPW11720.1"
                     /translation="MRPMLFNLGIGDGLFDSLCGALDAQAATLEIRSFPDGESYVRAL
                     DDCEGRVVTLACSLDRPNAKILPLLLAAETLRDLGAAQVGLAAPYLAYMRQDTRFKVG
                     EGITSRYFADLLSRHFDWLATVDPHLHRFHAMDEIYRVPARVVHAAPTLAQWIRQHVE
                     QPVLIGPDSESRQWVSEVAAGAGAPFLILDKQRSGDRDVRVSVPDLARHMDRVPVIID
                     DIVSTGRTAIAAVSHLATLGAPPPVCAFVHAVFADDAEAMLKAAGASRVVSCNTIEHP
                     SNAMDMSAPLAQACRDLMATSPERRR"
     gene            39496..40737
                     /locus_tag="PK399_00915"
     CDS             39496..40737
                     /locus_tag="PK399_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HPW11721.1"
                     /translation="MPPAHELTGLLAALGIGLLIGIERERSKGEGSTRGSAGVRTFCL
                     LALAGAVAALLGGVGIAVAGGFVVLTALASYRQTRANDPGLTSEVSMLLVFLLGMLAM
                     QEAALAAGVGVVVAILLASKTWLHRLVRQLLSTQELHDGLLLAGSAAIVLPLLPDRAI
                     DPWQVLNPRKLWLLAVLVMVINTAGHVALRMFGARLGLMLAGFAGGFVSSTATIASMG
                     SKSRELPALASTCATAGVLSNLSTVVQLALVTGMLSPPLLKQLALPLLAAAAVALGFS
                     LLAGWHGARADEPEVSTLTGRAFKPLHVLMFVAIVAAVMLFSAIIRDAVGTRALVWTL
                     ATSGLADVHAAAASAAQFVGLGRMDLRSATVAILFAFAANSAGKLVVAFVTGGHRYGL
                     RLLPGIVGMLAAFAVVVVFGW"
BASE COUNT         6350 a        13745 c        14075 g         6593 t
ORIGIN      
        1 gcgccgcgca gccagccttc gttccattgc acgtccggct tgaggccgcg caggcagaac
       61 agcatcaggc cgatgccaag catgccgtac acgccgaaca gcgcggtgtg gccatgcagc
      121 ggcgtcaggt tcaggccctg catgaagtac agcgacagcg gcgggttgat caggaagccg
      181 aacaggccgg cgcccaccag gttccagaag ctgaccgcga tgaagaacat gatcggccag
      241 cggtagcggg ccatccacgg cgtggccttg cccagcttgt aggtgtggta cgcctcgaag
      301 ccgatgtagg ccagcggcac cacttccagc gcgctgaagc tcgcgcccag cgccaccacc
      361 gcggtcggcg tgccgacgaa gtaaagatga tgcagggtgc ccagcacgcc gccgctcatg
      421 aacacgatgg tggcgaacag caccgcgacc gatgccacgg tgtccttgat caggcccagg
      481 cgggtgaaga tcagcgccat caccgcggtg gcgaacactt cgaagaagcc ttccacccac
      541 aggtggacca gccaccagcg ccagtactcg acttcggaaa tatgcgtgtg ctcgccccac
      601 atcagcgcgg aggcgaagaa caggccgatg cacaccgtgg acaggaacag caggccgacg
      661 atcgaacggg tgcccgtcgc cgggccacgc agcaccggcc acagcgcacg accgacgagc
      721 gtgagccaca gcagcaggcc gacgaacagg aaccactgcc agaagcggcc catgtcggcg
      781 tattcccagc cctggtggcc ccaccagaag ttgtgctcca ggcccagttt ctgcatcacc
      841 gccagccatt gcccggcgaa cgagccgacc acgatcagca gcaggcaggt ccacaggaag
      901 ttcacgccga gccgctggaa tttcggctca tgccccgaca tcgcgggcgc gatgtacagg
      961 ccggtgccga gccaggccac cgcgatccac agcacggcaa gttgcgtatg ccaggtgcgg
     1021 gtgatcgagt agggcagcac gtcggagagg gcgaagccgt aggcctgctg tccttcgacc
     1081 tggtaatgcg cggtgatcgc gcccagcagg atctgcacca cgaacaacgc cagcaccacc
     1141 cagaaatact tggcggtggc gcgcatcgac ggggtgatcc gaatcgccgc cagcgggtcg
     1201 ctggccggca gttgcagcgg ttcctcatgt gcctggcgcg catgccacca acccagcagg
     1261 ccgatgccgg cgatcaggaa cagcacgctg aaggacgacc acacccacat cgccggcgcc
     1321 ggcgtgttgt cgatgagcgg ctcgctgggc cagttgttgg tgtaggtgac ctgcttgccg
     1381 accacgccgg tcttcgacgg caccatcgca tcgccgggtt cgagcggacg ttcggtgccg
     1441 gcagcccagg ccgtccacca gaagaacgcg gtcagcgcgc gacgatgcga tgcatcgggc
     1501 acggtgtcgt tcttcatcgc atacgcctcg cgcagcgtgg cggtggcggg gtcgttgccg
     1561 aacaggctct cgtagtgcgc ggccacgttg gacagcgcca gcgcgcggtc atccggcagg
     1621 gtgatcgtgc cggtcgcggc gtcgtaggtg ttggtgcgca tcacctgctt caggcgtgcg
     1681 cgcaacgctg cctgctgttc ctcggggagt tgcgcgtagg tggccatgcc gccatccgcg
     1741 cgtgcccaca ggtcgagcag cgacaccgat tcgcgatgca gccagtccgc gctccagtcc
     1801 ggcgccacgt aaccgccatg accccagatg gaacccagct gcatgccgcc catcgactgc
     1861 cagacctggc gcccctgctc catgtcggcg cgggtgtaga tcacctggcc gctctcgctc
     1921 agcactttct ccggcatcgg cggcgcggtg cggaagatct ccgtgcccgc ccacagcaac
     1981 accccgaacg acagggtcag caagactgcc aaccccagcc acaatttcct ggtcgcgctc
     2041 atcacggcat ctccatggtg tgtcgccatg gtcgtgtcgc gcgttgcgca tcgcattgat
     2101 gcaggtcaag cctgcgggca acgcctcact cgcgcaggat cggtgccgcc agggcttcga
     2161 tgcgctcgcg atcgagcagc gagacggcgc ggcgttcgac cgccagcagg ccgtcgtcct
     2221 gcatgcgccg cagcacccgg ctcacggttt ccggggccag ccgcaggtag ttggcgatgt
     2281 cggtgcgggc catggtcagc tggaagtggc tggccgagaa cccgcgcgcg gcgaggcgcc
     2341 gcgacatccc gaccaggaac gcggcgacgc gctggtccgc actccagtcg ccggcgagca
     2401 atgccgcgcg gccgatgtcg cggctgagca ggcggaacag ctcgcgctgc aggccgggga
     2461 cgcgcgcggc cagcacggag atcttcggga acgagaaccg gcacagcatc accgtgtcca
     2521 gcgccaccgc gtcgcacgga tagcggtggc cgtcgatcgc gttgaggccg atcacctcgc
     2581 ccgggaaatg gaagcccagc acgtgctcgt ggccatcacg gtcggtgacg caggtcttga
     2641 ccgtgccggc gcgcaccgcg gcgatcgcct cgaacggatc gccctcgcgg aacagcacgt
     2701 cgccggcgtg gtacgggccg acatgctcga ccagcacgtg caggtccatc agcgccgcct
     2761 tgtccatgcc ctgcgacagg caggcgtgcg agaacgcgca ggtggtgcag aactgcaggt
     2821 cgctgccgtc gtcggccagc acgcgcggat tgaaccgcgg gaacggcacg ctgctcacgg
     2881 catcgtccac ggacgggtca gatcgcgcgg gagaagcgcg gcctggcatc gatgacggat
     2941 gtctggtcga ggtaacggtc gaagcacatg gcaatgttac gcaacaacag gcgtccgcgt
     3001 ggcgtggcgc ggatcgcgtc cggaccgacc tcgaccaggc cgtcgtcggc cagcggccac
     3061 aggcgttgca ggtccgcggc gaaatattcc gcgaagccga tcccgtggcg ttcctggaac
     3121 gcgcgcacgc gcacttcgcc ctggcacatc agcgactgga tcaggtcggc gcggatctcg
     3181 tcgtcctcat ccatgcgcat gccgcggaac accggcagcc ggcctgcatc gagcgccgtc
     3241 tcccaggagg gcaggtcgcg cgggttctgg ctgaagctgc cgccgatgtg gctgatcgcg
     3301 ctgacgccca ggccgaccag gtcgctgtcg gcgtgggtgg tgtagcccat gaaattgcga
     3361 tgcaggccgc cacgcgcctg tgccaccgcc agttcgtcat cgggcagggc gaaatgatcc
     3421 atgccgatgt agacgtagcc ggcggcggtg aggtgttcca ccgcggcctg cagcagggcc
     3481 agcttggtgt cggggtcggg cagcagcgca tcgtcgatct gcgtctgcgg cttgaagatg
     3541 tgcggcaggt gcgcatagct gtagaccgcc agccggtcgg ggcgcatggc caccaccgtg
     3601 tccagcgtct tgcggaatcc gtccaggctt tgcccgggca ggccgtagat caggtcgatg
     3661 ttgaccgagc gcatgcccgc gccgcgacag gcgtccacga tcgcgcgggt ctcttccacc
     3721 gattggatcc ggttcaccgc ggcctgcacc ttcggatcga agtcctgcac gcccaggctg
     3781 gtgcggttga agccggccgc ggccagcacc ggcatgtccg ccgggttgca gaagcgcggg
     3841 tcgagttcca ccgagatgtc gcggtcgtcg ctgtcggcga agcggaatgc gccccgcagg
     3901 gtctcgacca cgccgcacag ctgcgccgcg tcgaggaagt tcggggtgcc gccaccgaag
     3961 tgcagctgga tcacctcgcg atcgcggtcg aacagcggcg ccaccagggc gatctcgtgg
     4021 cccagccggt ccaggtaggc gcggccgcgc gccaggtcgc gggtgatgat ccggttgcag
     4081 ccgcagtaga agcaggggct gaagcagaac ggcacgtgca cgtacagcga cagcggtcgc
     4141 gggatcggat cctcgttgct ggcccgggcg acctcgcgca gagccgcttc gccgaagtcc
     4201 gcgctgaact ggggcgcggt cggataggag gtgtaacgcg ggccgggccg gtcgtagcgg
     4261 cgcagcaacc cggcgtcgaa ggcgatctgc tgggacggca tcggcgcagc ttaggcaggc
     4321 ggcggccgcg gcgaattgac ttcgatcaac tcaggcccaa gtcgctcagc ggcgctgcag
     4381 cgcgtagacc gccgtgtcca gccgcgagac ccccgcgacg aagcccggat ggtcgggcgg
     4441 gactggcctg cgttcgcgca gttcgacccg ccagtcggcc tcgtacaacg cctgcacttc
     4501 gtcctgcggc acggagaacg gcgggccctc gcgttccgcc tgcgggtatt ccagggtgac
     4561 cagcaggccg cggcagcctg ccggcaacgc cgcgtacagg tcgcgcgcat agcgcagccg
     4621 gacgtccgcc ggcaaggcga tcagcgccgc gcgatcgaac gccgcggcgc agtcccgcaa
     4681 cagctcggca tccagcccga aggcatcgcc cacgaccagc tcgatgccgc cggcctcgta
     4741 gtggcgcccg tagcgggact cgcgcgtgtc cggctgcagg ccgtgctcgg cgaagaactg
     4801 ctccaccgcc aacggcgaca actccacgcc cagcacgcga tggccctgcg atgccagcca
     4861 cgccatgtcg cgcgacttgc cggcgagcgg cacgaacacc ttggccccgg gggcaacgcc
     4921 cagcgagccc caatgcttca gcagcagggg cgtgggggcg tcctggtgga agccgatctg
     4981 gttgtcggcc cagcgctggt gccagaaatc ggggtgcatg aggggtgatg tcttgtctgc
     5041 ttaagcaacg gtgttggagc gactacaggt ggtcattcga tcgccagccc gtcgactttc
     5101 tgccacccgc gcggcagcag gccgccgcgc gagccgcggg cacccaggta atcgtcgagc
     5161 tccttgaacg acagcgtcat cgtgcgcgcg ccggacttca cctgcagctt gccggcggcc
     5221 ggcaccaccg cgatggcgac gacgtgttcg gtgccgcgct tggccttggg gatctcgatc
     5281 agcttgttgc ccttgccctt gtccagttcc ggcaggtcgg ccaaggcgaa ggccagcacg
     5341 tggccggcgc tggtcaccgc gaccacgcgg tcggtcgcca tgtcgaccac cgccgccggc
     5401 tgcagcacgc gtccgccctc ggacagcgag agcagcgcct tgccggcctt gttgcggccg
     5461 gtgaggttct cgaagcgggt cacgaagccg tacccgtggc tggaggcgat caccaggcgc
     5521 atgtcgttct cgccgctggc catggcctgg aaaccggccc cggccgccgg cgtgaagcgg
     5581 ccggtcagcg gttcgccgtt gccgcgcgcg gagggcaagg tgtgcaccgc ggtcgaatag
     5641 ctgcggcccg cgccatcgag gaacgcaacc tgcaggttgc tgcgcgccct gaccgccgcc
     5701 agcaggccgt cgccgtcgcg gtaactcagg gcggcggcgt cgatgtcgtg gcccttcgcc
     5761 gcgcgcaccc agcccttctc gctgagcacc acggtcatcg gttcgctggc gaccagctcg
     5821 gtttcgtcca gggcgtgcgc ggcctcgcgc gcaaccagcg gcgagcgccg tgcatcgccg
     5881 aatttcttcg catcggccag cagctcgtcc ttgatcagct tcttcagctt cgccttgctg
     5941 ccgagcaggg tcatcaggcg gtcgcgttcg gcgtcgagtt cgtcctgctc gccgcggatc
     6001 ttcatttcct cgagacgggc gagctggcgc agcttggtgt cgaggatgta gtcggcctgg
     6061 tcgtcgctga ggccgaagcg cgcgatcaac gccgccttgg gttcgtcctc gctgcggatg
     6121 atgcggatca cttcgtccag gttgaggaac gcgaccagca agccttccag caggtgcagg
     6181 cggcgctcga ccttctccag tcgatgggtg aggcgacggg tgaccgtggt ctggcggaac
     6241 tccagccatt cggtcagcag catcttcagg tgcttgacct gcgggcgccc gtccaggccg
     6301 atcacgttca tgttgacccg gtagctcttc tccaggtcgg tggtcgcgaa caggtggccc
     6361 atcagctgct cggcatccac gcggttgctg cgcgggacca gcacgatgcg ggtcgggttg
     6421 gcgtggtcgg attcgtcgcg gatgtcctcg agccagggca gcttcttggc ccgcatctgg
     6481 ttggcgatct gctcgatgat cttgctcggg ctgacctggt agggcagctc ggtgatgacg
     6541 atgttcgcct gttccttcac gaacacgccg cgcgcacgca cgctgccgct gccggtctcg
     6601 tacatcgcct gcaggtcggc ggcgggggtg atgatttccg ccgcggtcgg gtagtcgggg
     6661 cctcgcacgt gctcgcacag gtcgcgcacg gtcgcatcgg gatcgtcgag caggcgcacg
     6721 caggcgctga cgatctcgtt gaggttgtgc ggcgggacgt cggtcgccat gccaacggca
     6781 atgccggtgg tgccgttgag caacaggtgc ggcaaacgcg ccggcagcca ggtcggctcc
     6841 tccagggtgc cgtcgaaatt cgggtcccag tccaccgtgc cctggccgag ttcgcccagc
     6901 agcacctcgg cgatcggcga cagcttggat tcggtgtagc gcatcgccgc gaacgacttc
     6961 ggatcctcgc tggagccgaa gttgccttgg ccctcgatca gcgggtagcg atacgagaac
     7021 ggctgcgcca tcagcaccag cgcctcgtaa caggcgctgt cgccatgcgg gtggtacttg
     7081 ccgatcacgt cgccgacggt gcgcgccgac ttcttcggct tcgcgcccgc attcaggcca
     7141 agttcgctca tcgcgaacac gatccgccgc tgcaccggct tcaacccgtc gccgatgaag
     7201 ggcagggcgc ggtccaggac cacgtacatc gagtagtcga ggtaggcgcg ctcggcgaat
     7261 tcgctgagcg ggatctgttc gaagccgtgg aaggaggaac ggacgggggt gtcggtcatt
     7321 caggcgggca ggtgcagggg aacccgcatt ctagcgaggc aacgggatgg cccggtgccg
     7381 ctcagcgatg caggtcgccg gcggcctcgg gctggtagcg gaccgccagc acctgcaggc
     7441 gcgcgttgcg cccaccgggc aggggccagt cgatgctgtc gcccacgcgc aggcccagca
     7501 gggcgctgcc gaccggcgcc aggatcgaga ccctgtcggc gctgccgtcg gcctggcgtg
     7561 ggtacaccag ggtcagttca tgctcgtgcg cgccctgcat cggatcgtcc acccgcacgc
     7621 gcgcgatcga gttcatggtg atcacatccg ccgggatcgc ggcgggttcc gccagttccg
     7681 cgcggcccag ttcgccctgc agggccgccg tgtccagcgc gcggaaggct ggctgctcca
     7741 gcagggcctc gatgcgttcc acgtccaggc gcgacagcaa cagcggcggg cgggaagcgg
     7801 cggcggtgga tggacgggcg tgcatgtgcg ggctcctcaa agcagagcgg gcgacacggc
     7861 atgcgcgtcg cccgcaggtg tacgaaggtg gtggggcgac catagccccg aacggaaggg
     7921 gcggcaagcc gaacggtgca tggatgccgc gacgcttgag ctgccggcta tgctgcgcgt
     7981 cacccccatt ccgaccaagc cgccatgtcc aatccccgga tccagcaact ccagcagctg
     8041 ttcgtctccc gcctcgacac cctgtcgcac atcctgcagg tcggccaggc gcacctgggc
     8101 gatcgcgacc tggtcaacga gcggctggcg ccggacatgc tgccgctggg cgcgcaggtc
     8161 gcgttcgcct gcaaccagcc gcgcgggttt tcgcaatggt gcgccggcca gccgatcgag
     8221 aacatcgacc cgacgaccgt ggcctcgctg gaggacgcgc atgcgcagat cgccgacacc
     8281 cgcgcgcggg tgctcgccat cagcgcgagc gatgccaagc tggatgaagt gaagcgcatc
     8341 ggcatgggcc cgggacgcta ctgcgaactg cccggcgacc tgtacgtcag cgaatacctg
     8401 ctgcccaacc tgtatttcca catcaccacc gcctacgcga tcctgcgcat gctcggcgcg
     8461 ccggtcggca aggccgacta catgcgctac ctcggggcgc atctccgcca cgacgggtga
     8521 gcaatggata atcgcgggat cgcaaccgaa ttcctccggc tgtgcgcgcg cggcgacgtg
     8581 cgcgaggcct acgcgcgctt cgtgtccgac gatttcgtcc accacaaccc gtggttcgcc
     8641 ggcgaccgcg aatccttgct ggtcgcgatg gagcagagtg cggccgccga gcccaacaaa
     8701 tcgttggaag tgaagcaggt catcgacggt ggcgaccgcg tcgccgtgca ttcgcacctg
     8761 acgcgcgccg acgccggcct gcaatatgcg gtggtccaca tcgcccggat cgaacacggc
     8821 aggatcgtgg agctgtggga cctggcgcag gaacttcccg gcgaatcgcc gaacgcgaac
     8881 gggatgttct gagcgttcgt tcagcttcaa tccggtgctg gacgcggtct tcaccacgcg
     8941 gtcgcgatgg tgcgggaaag cacccacgga ggaagcgcat gaagatccgc cacgtattcg
     9001 tgacccgtga cgtcatccag gcgcgcgatg ccttgctggc gctgcgcgag caaggcgtcg
     9061 gcgtggacga catttcgctg gtggcgcgtt ccgacatcga actggaccgc atcccggacg
     9121 agctcaagga agcggacacc gacttcgtgc cggcggccat gcgcggcatg ggcatcggcg
     9181 gcgccaccgg cctgctggcg gggctggcgg ccgtggtctt cgccccggtc ggcatcaccc
     9241 tggccggcgc ggcggcgatc ggcgcgctgg gcgcgctggt cgggggctgg tcatcggcgt
     9301 tgatgggttc ggcgctgccg gatccggtgc gccagcagtt cgacgacgag atttccgccg
     9361 ggcggatcct gctgctggtc gatgccgagc ccgacgtcca gccgcgcctg caacttgcgc
     9421 tggcagcggt cggtgcccgg cggctggact atgaggcccc ttccgcgatg acctgatcga
     9481 cggcggaatc cgaccgggtg catccatcaa cccgtgcgtt ccgtatgtcc ttgcatgcag
     9541 gatgtccgca ttgcgacagg aggcgacgga tgccagggtc ttggatgaag gcgcgacacc
     9601 tcgccgtcgc gtgcggcgtt gcatttgccg cggcaacgac ggcggcctcg gccaacgatg
     9661 cgcctgctgg cggcggcgtg ctgcgctacg tcggcgaggc gcgtgatcca ggcgggcggc
     9721 tgctgtatcg cgaaacccat tacctggatt cccgcgcgcc cgcgccgcgt cgggtggtgc
     9781 tgtaccagtg cgcggacggt cgcccgttcg cccgcaagac cgtcgacggg gcaggccccg
     9841 caccggcatt cgccttccta gacgcgcgtg acggttacca ggaaggggtg cgcacccagg
     9901 gcggcacgcg cgaggtgttc tggcaggaag gcaaggcgtc gccgctgaag cgcgccgccg
     9961 tcgccaagac cggcgcgacg gtattcgatg ccggtttcga tgcctatgtg cgcgagcatt
    10021 tcccggcatt gcagcagggc aaggccgtgc gcgcgcggtt cctgatcccg agccggcaat
    10081 ccgcgatcgg cgtcagcgtg aagccggtcg ccggcgcacc ggccggctac ctcgacctga
    10141 agacccgcct ggatgcgtgg tacggcttcg ccttgccgga gctcaagctg cgctatcgcg
    10201 tcgccgatcg ccggctggtg cgcttcgagg gcatcggcac gattcgcgat ggcaagggcg
    10261 ggcacggcaa gctgcggatc gatttcgcgc aaccggcgga ggcgatcgag gccggcgagt
    10321 tgcagcgcgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cagcgcgctc
    10441 cacgtgggtt ccggcttgcc gcattcacgc cctgcatggc ataaggtggc tcgtttccat
    10501 cgacggcccg ccgccagccg gcatgcccgc cagccggacc accgcatgag tgcaataccg
    10561 ctgcagcaat ccctggacgg cagcgccgat ccgttggccg cgctgctcgc gcaggtcgcc
    10621 gaggggcgcc gcgacgcctt cgaaagcctg taccggcaga cctccgcgac catgctcggg
    10681 atctgcctgc gcgtgttgcg ggatcgcgag gaagcggagg acgtgctgca ggaagtctat
    10741 gtggcggtgt ggtcgaaggc cgcgcagttc gaccagcagc gcgcgcgcgc gatgacctgg
    10801 ctgggcacca tcgcccgcaa ccgcgccatc gatcgcgtgc gcgcccgccc gtcgcctgcg
    10861 ttgcacgaac ccatcgaaga ccatgagctg gccgacgacg aggcgcccac gcccgccgcc
    10921 ggcatcgatg ccgcgctcga gcgtgcgcaa ctcgacgact gcatggatca gcttgaaccg
    10981 aagcgtcgct cgctgatccg caccgcgttt ttcgaaaacg tgacctatga cgtgctggcg
    11041 cttcgtgccg aagcgccgtt gggctcggtg aagagctgga tccgtcgcgg cctgcagcaa
    11101 ctcaaggcgt gcctggaacg atgaacgaca tcgcgcccgc gttgcccccg atcgacgacg
    11161 acgacctgct ggccggcgag tacgcgctgg gcgtgctcga tccggatgcg cgccgtgcca
    11221 cgcagcgccg gatcgacgcc gaccgcgatt tcgcggcccg ggtcgcgcgc tgggatgcgc
    11281 atttctcgcc ctggctgctg cgggtcgatg cggtcgagcc cggggcgcag gtctggccaa
    11341 ggatccgcac ctcgctgggg tggtcggccg tggaaggcca ccgcaagggc ctgtgggaca
    11401 acgccgcgtt ctggcgcggg accacggccc tggccgttgc cgccagcgtg ttcgcggtgg
    11461 ccatcggcgt atggcgcacg ccggagccgg cggcacccgt tccgccgcct gtggtcgtgg
    11521 tgcagccacc gcccgcgacc gacgaagcgg ctgcactgcc ggtcaccgtg ctggccaccg
    11581 acgacggcaa caccggctgg atcgcgcggg tcgcgccgga ccgcagcaag gtgctgatgg
    11641 tgcccaagcc gcgcccgctc gatgccggcg gcaaggtcaa cgaactctgg atcatcgcgt
    11701 cgggcgaggc accgaagtcg ctgggcttcg tgtccaacga caaggcgcat tccatcgaca
    11761 tcccggccac gctgcgtgat gcggtggcgg cgggcgcggt gttcgcggtc accctggagc
    11821 cggagcaggg cctgccgcat gcggcgccgt ccggcccggt cgtcgccaag ggccagatcc
    11881 tgtccatctg agcctggcga cccggtcacc gggcactgca tccgaacgcg ggttccgtcc
    11941 gtatatccgg caacgggacc cgccaaggaa tgccatgccg cccaagcctg actccacgaa
    12001 cgcgccgcgc ggcagcgcgt tgctgcgctg gatcgacaca tcgggacagt ccggatcccc
    12061 cgatccggcc aacgatgatc gcatcgactg gctgcgcgcg ctgccgttcg tcggcatgca
    12121 cctggcgtgc ctgggcgtgt tgtgggtcgg cgtgtcggct accgccttgt gggttgcgct
    12181 ggggttgtac gcgctgcgca tgttcgcgat caccgggttc taccaccgct acttctcgca
    12241 ccgcacgttc cgcacgtcgc gcgcggtgca gttcgcgttc gcgctgctcg gcgcggcgtc
    12301 ggtccagcgc ggcccgctgt ggtgggcggc gcaccatcgc catcaccatg tgcatgcgga
    12361 taccgcggcc gacccgcata ccccgcgcaa gggcttctgg cgcagccacg ccggctggtt
    12421 cctgacccgc ggcggcttcc gcaccgacac ctcgcgcatc ggcgacctgc tgaagttccc
    12481 ggagctgcgt tggctggatc gcttcgatac cgcggtgccg gtcgcgctgg cggtgctgct
    12541 gttcctggcc ggcatggcgc tggagcgctg ggcaccgggc ctggggacca acgggccgca
    12601 gttgctggtc tgggggttct tcgtctccac ggtggtgctg ttccatgcca ccgtcaccat
    12661 caattcgctg gcgcatcgct ggggccgtca gcgcttcgac acccgcgacg acagccgcaa
    12721 caacctgtgg ctggcgctga tcacgttcgg cgaaggctgg cacaacaacc accatttctt
    12781 cccgggcagc gcgcgccagg gattccgatg gtgggagatc gacctgacct ggtatggcct
    12841 gcgcacgctg caggcgctgg gcctggtccg tgacctcaag ccggtgccgg cgtgggtgct
    12901 ggcgaaagcc gatgcgaaca aggcgggtcg ctgagatgcg catcgcgatc gtcggttccg
    12961 ggatcagcgg cctgtcggcg gcgtggttgc tgtcgcgcca acacgacgtc gtcctgttcg
    13021 aggccaacga ttacctcggc ggccacaccg acacccatcg ggtcgaagtc gatggccgca
    13081 cgctcgacgt ggacactggc ttcatcgtcc acaaccccgg caactatccg ctgctgaccc
    13141 gcctgttcga cgaactcggc gtggccacgc aatcgaccac gatggggttc tcggtgcagg
    13201 acgcgcgcac cgggctggaa tacaacgcga cctcgctcga cgggctgttc tgccagcgcc
    13261 gcaacctggc ctcgccgcgc ttctggggca tgttgcgcga cttggcgcgc ttctatcgcg
    13321 aagcaccggc cttgctcgac gacgaccacc cgggcccttc gctgggcgag tacctcgacg
    13381 ccaacggcta tggcgctgcg ttccgcgacc agcacctggt gccgatggcc agcgcgctgt
    13441 ggtcctcgcc cgagcagtcg gtgctggcgt tcccggcgaa atacctggtc cagttcatgg
    13501 ccaaccacca catgctgcaa ctgaccggcc gcccggagtg gcgggtggtg cgtggcggtt
    13561 cgcggcgcta tgtcgaggcg atggtctcgc actgggacgt gcaggtgcgg ttgtcctcgc
    13621 cggtgcgcgg catcgcccgc gacgcccatg gcgtgagcgt gcgctgcgat gccggcagcg
    13681 aacgcttcga ccaggtcgtg ctggcctgcc acagcgacca ggcgctgcac ctgctggatg
    13741 atgccagcga tgccgagcgc gacatcctcg gcgcgatccg ctaccaggcc aacgacaccg
    13801 tgctgcatac cgatgcgcgc ctgctgccgc gcaaccgcaa ggcatgggcg gcgtggaacg
    13861 cgcacctgcc ggcggatccg gcgcaggcct gcaccgtgag ttactgcatg aacctgctgc
    13921 agggcctgcc cggcgacacc ccgctggtgg tcaccctcaa ccgcacgcac gacatcgacc
    13981 ccgcgcgcat cctgcagcgc cgccagtacg cgcatcctgt gcatacgcac gccgcggttg
    14041 ccgcacaggc gcgccgccac gagatccagg gcgtgcagcg cacctggttc gccggcgcct
    14101 actggggctg gggcttccat gaggacggca tgcgcagtgg cgtggaggtc gcgcagggaa
    14161 tgggcgttgc gtggtcgaac gatcaggtgc gtcgcggcgc tgtcgacacg cagccgctgg
    14221 aggccgcggc atgaccgtac tggccagtgc gatctacgaa ggccgcgtgc gccatcgccg
    14281 gcatgcgccg cgcgcgcacg cgttttcgta tcgcatggcc cagctctacc tggacctgga
    14341 cgagctcgat gcggcgttcc gcgggcgctg gctgtggtcg gtggggcggc gcaacctggc
    14401 cgcgttccat cgccgcgact tcctcggcga tccggcgcgt ccgctggccg atgcagtgcg
    14461 cgatcgggtg gaggccgcca ccggcgaacg tccgcttggc gcggtgcgcc tgctcaccca
    14521 cctgcgctat gccggcctga gcttcaatcc ggtgagtttc tactactgct tcgatgccgg
    14581 cggcacgttg cacagcatcg tcgcggagat caccaacacg ccgtggaagg aacgccatgc
    14641 ctacgtgctg ccggtggcga ccgcggagcg ccggggcagg gcactgcact ggcgtttcga
    14701 caaggccttc cacgtctcgc cgttcctgcc gatgcagcgc cgctacggct ggacgttcac
    14761 cgagccgggc gacggcctgc aggtgcacat ggacgtctgc gacggcagcg agcgcgaatt
    14821 cgatgccacc ctgcaactgg aacgccgccc actgaccggc gcgaacctgg cgcgcgtgct
    14881 ctggcgctat ccgctgatga ccgcgcaggt ggtcgtcggc atccactggc aggcgctgcg
    14941 gctctggctg aagcgcgtgc ccgtgcatga ccaccccacc cgaatggaat cgccccgatg
    15001 aatgcacgtg ccgatgcctc gcccgcgacc gggttctccg cgctggaccg cctcctgcgc
    15061 aaccgcgtgc tggaccagtt gcgggccttg cggggccgcg cgatcgtgct ggccgatccg
    15121 ctgggctgca tccgcaccgg tgatgcggaa gacgccgctg caccgctgcg catcgacgtg
    15181 cacgggcccg gcttctatcg cgcgctggcc gccaacggca gcgtgggcgc ggcggagtcg
    15241 tacatggacg gcgagtggga ctgcgccgac ctggtcggcc tggtgcaact gctggtacgc
    15301 aaccgcgacc tgctggacgc gatggagcgc gggcccgcac gcatcggtgg gtgggcgatg
    15361 cgcggctgga acgcactgcg ccgcaatacc cgcgatggcg cgcgccgcaa catcgccgcg
    15421 cattacgacc tgggcaacga cttcttccgg ctgttcctgt ccgaagacct gatgtactcc
    15481 tccgcgctgt atgcgggcga cgacgacacc ctggaagccg catcgacccg caagctcgac
    15541 cgcatctgcg acaagctgcg gctggggccg gacgaccacg tggtcgagat cggcaccggg
    15601 tggggtggct tcgcggtgca cgccgcacgg acgcgtggct gccgggtgac caccaccacg
    15661 atctcgcgcg agcagcatgc gctggccagc cagcgggtgg ccgatgccgg cctgcaggac
    15721 cgcgtcaccg tgctgctgtc ggattaccgc gacctgcagg gccagtacac caagctggtg
    15781 tcgatcgaga tgatcgaggc gatcggcgcg cagtacctgg acacctactt cgccaccctg
    15841 gggcgcttgc tcaagccgga cggactggcg ctggtgcagg cgatcaccat cgaggaccac
    15901 cgctacgagc aggccctgcg cagcgtggac ttcatcaagc gccatatctt ccccggttcg
    15961 ttcattccgt cggtcgcggc gatgctggcg gcgaagacgc gcagcagtga cctcgcgctg
    16021 gtgcacctgg aagacttcgg gccctcgtat gcgcgcaccc tgcacgattg gcgcgagcgc
    16081 ttcctggccc gccgcgagga cgtccgcgcg cagggcttcg acgagcgctt cctgcgcatg
    16141 tggaccttct acctggccta ttgcgaaggc ggcttccgcg agcgttcgat cggcgtcgcc
    16201 catgtgctga tggcgaagcc gggctaccgc ggcggctcct acctgcccgg cttgctggag
    16261 gcctgatgcg cttctggctc aacctgctcg gttatcaggc ggcgtggttc attgccgtcg
    16321 gctttgccgc gcgcgggctg gcatggccgg gcatgctggc ctgcctgggg tttgccgcga
    16381 tcagctggtg gacgtcgccg ctgcgtcgtt ccgacctgaa attgatcggc gcggcactcg
    16441 cctgcggcct gctgctggac ggcgtgctcg ccgccaccgg ctggctgcgc tatgccgcgc
    16501 cgttgccggc gttgccgggt ccggcctgga tcgccacgtt gtgggtcgcg ttcgcgatga
    16561 ccctgcagca ttccctgcag tgggtgatcg cgcggcccgc cgttgccatc gcctttggcg
    16621 tgatcggcgg cccgctggcc tactggggcg cgtcgcgcgc cttcgacgcg gtcgtcttca
    16681 tcatgccgct gcaggccacc ctggccctgg cgctcggctg gggcatcgcc atggccgcgt
    16741 tcgtgctcat cgtccatcgc cgccacgctt ccatcaccca tcaacccggg gtatccgcat
    16801 gactccatgg cagcaactcc tcctggtctg gctggtcgca gtggtggcgc agtcgcttgg
    16861 ctggctctgg caacgcaagc gcagcaatgc cggcatcgtc gacgtggtct ggtcgttcgg
    16921 cgtgggtggc gcggcggtgt tggtcgcggc gaccggcagc ggcgcgctgt tgccgcgcgt
    16981 gttgctggcc atgctcggcg gcgcctgggg cctgcggctg ggcctgcacc tgtggcatcg
    17041 cgtgcgtgcc gagccggaag acggccgcta cgcgcaactg cgccagcgct ggggcaacga
    17101 ccagcgcaag tggttcgcga tgttccagtt ccaggcgctg ctgatcgcgt tcttcgccct
    17161 gccgttcctg gcggtggccg cgaatcccgt cgccgactgg aacgcgtggt tcatggccgg
    17221 catcgccacg tggctggcct gcgtggcggg cgaagcggtg gccgatgcgc aactggcgaa
    17281 gttccgcacg aatcccgcca acaagggcaa gacctgccga gacggcttgt ggcggtattc
    17341 gcgccatccc aattatttct tcgaatggct gcactggctg gcctacgtgc tgctggcggt
    17401 gggctcgccg atcgcctggc tggcctggag cggccccgtg gtgatgtacg tgttcctgcg
    17461 ctggatcagc ggcatcccgt tcaccgaggc gcaggcgctg cgcacgcgcg gcgaggacta
    17521 cgcgcgctac cagcgcagca cgccgatgtt gttcccgtgg ttcccgaagg aggatcgatg
    17581 aacgccaccg ccgatgcgat ggaactggcc agcgaccgcc cggccgacgg cgtgctgggc
    17641 ttggccgaac gcggcctggt gccggatgcg ctgctgcgcg ccggcatccg ccggatgtgc
    17701 gcgcaacgcc tccgcgatga acatgccggc gatcccgatg ccgccgcgcg ccgcaatgcc
    17761 gcgttgatcg cggaactgcg cgaaagcccg gttgccatcc acaccgatgc cgccaaccgc
    17821 cagcattacg aactgccgcc ggggttcttc acccgttgcc tcggtccgcg gctgaagtat
    17881 tccggctgct actacccgac cggcaacgaa accctggcgc aggcggagga ggcgatgctt
    17941 gcgctgtacg gcgaacgcgc ggaactcgcg gacggccagg acatcctgga gctcggctgc
    18001 ggctggggct cgctgaccct gtggatggcg cagcactatc cgaacgcgcg catcaccgcg
    18061 gtcagcaact ccgcgccgca gcgcgccttc atcgaggcgc aatgccgcga gcgcggcttc
    18121 gccaacgtgc gggtgatcac ctgcgacgtg aaccggctgg agttgccggc atcgaacttc
    18181 gaccgctgcg tgtcggtgga gatgttcgag cacatgcgca actacgccac gctgctgggg
    18241 cgcatcggcg gctggctgcg accgggcgga aaactcttcg tgcacatctt ctgccaccgc
    18301 acgctgttgt atccgttcga gaccgcaggc gacgacaact ggatggggcg gcatttcttc
    18361 accggcgggc tgatgccggc ggcggacacg ctgctgtggt tccaggatgc gttgcgcatc
    18421 gaggatcgct ggctggtcga tggcacccac tacgagcgca ccgccaatca ttggctcgag
    18481 aaccaggatg cccgccacga cgaggtcatg gcgatcctgc gcgaggccta tggcgatgcc
    18541 gccaggctct ggttccagcg ctggcggatg ttctggatgg cgtgcgcgga actgttcggc
    18601 tacgaccacg gccgcgaatg gatggtcgcc cattaccgct tctcgaagga ggtcgcatga
    18661 tccgcaaact gttcgtcgca ctcggcatcg ggacattgct gtccgcctgc gcctccaccc
    18721 cgccgaccat cccgcctgtc gccagcgtcg acctgccgcg cttcatgggc gactggtacg
    18781 tcatcgccca gatccccacc tacatcgagc gcaaggcgtt cgattcggtg gagtcgtatc
    18841 gcctggacga ggacggcaag atccgcacca ccttccgtta tcgcaacggc agcgccgatg
    18901 cgccgctgaa gacgatggag ccggtcggcc gcgtggtgcc cggcaccaac aacgcggtgt
    18961 gggagatgca gttcgtgtgg ccgatccagg ccgagtacgt gatcgtggac ctggccccgg
    19021 attactcgcg caccatcgtc gggcgcagca agcgcgacta cgtctggctg atggcgcgca
    19081 cgcccagcct gcccgcgccc gaactcgccg ccgccatcga gaagatccgc gtgctcggct
    19141 acgacaccgc caagctgcgg atggtcccgc aggcagcggc gaacgcgccc gcaccctgat
    19201 cccgccacgc cgcccaacgc ccggacgcac gcatgaccca aggcctcttc tggtttcgcc
    19261 gcgacctgcg cctggccgac catccggcgc tggcgcgtgc ctgccagtcg cacgacaggc
    19321 tgctgctggt gtacgtggac gatgatgccg acggcccctt cgacgccagc cgcgcgtggt
    19381 tgcgcctgtc gcttggaaaa ctcggcgatg acatccgcgc gcgcggcggc gaattgcacc
    19441 tgctggccgg tgatccggaa atcctgatcc cggcgttggc ggtcgcggcc ggggccaccg
    19501 aggtgcacat ctccgccttg cacgaaccca aggccgccgc gcgcgatgca cgcatcgccg
    19561 ccacgctggc cggacgtgcc atcgccatgc gccgcgccgg tggtcgcctg ctgaccgatg
    19621 cggagtcagt gctgacccag tcggccacgc cattccgcgt gttcacgccg ttccacaagg
    19681 ccgcgcggcc gacgtggcgg catcgcccgc gcgaagcgcc caccgcgctg gcgtgcagtc
    19741 cactcccgtc atcgctggaa ggcgcgcgca ccacgctcga tgcgccacgc ccgcgttggg
    19801 acaagggctt ctgggccacc tggacgccgg gcgaagcggc ggcacacgcg cggctcgacg
    19861 acctgctgcc gcgcctgcac gcgtatccgg atgcgcgcga cgtgccgtcc gtcgagggca
    19921 cctcgcggct gtcgccgcac ctgcatttcg gcgagatcca cccgagccgc atcttcgacg
    19981 tcgtgcaacg gcagggtggc atcggcgccg acaagtacat cgccgagctc ggctggcgcg
    20041 agttcgccta ctacgtgctg caccactggc cggccagcct ccacgacaac ttcaatccga
    20101 agttcgacgc catgccctgg cgcgacgatc ccgccggact cgaggcctgg cagcgcggcc
    20161 tgaccggcgt gccgctggtc gatgccggca tgcgccagtt gtggcacgag ggctggatgc
    20221 acaaccgcgt gcgcatggtg gtcgcttcgt ggctgaccaa gcacatgggg atcgactggc
    20281 ggcagggcgc cgcctggttc atgcacaccc tggtcgatgc cgacctggcc agcaacacgc
    20341 tgggctggca atgggttgcc ggcaccggcg tggatgccgc gccgtatttc cgcatcttca
    20401 atccggtcac ccagtcgcgc cgcttcgatc cgcagggcgc gtacctgcgt cgttggcttc
    20461 cagaactgcg agggcttggc gatgacgcca tccacgcccc gtgggagcgt ggactgcgcg
    20521 tgcccggtta tccggtcaag cccatcgtgg acctgggtgc cggtcgtgac gctgcattgg
    20581 cgcggcttgc agcattggcg aagtaacgga agaactgcct ccgccctcgc agtcgcgaag
    20641 atgcctgcgg ccagccgggc ctgcgcgatg cgacatcaac cgcccgtgcc gaacttcggc
    20701 atttccttca gcggcagcgc gtcgaacgag aacgtgccgc tggcggaggg caaggttttt
    20761 gccggcagct tcttcgccag ggacgaagcc ggcacgtcct tcgccgtgaa actgccggcg
    20821 atcgcgtagg tgccatcggc gttgcgctcg aaacggtcga tcgtgaccgt gacctgttcg
    20881 ccgcccttgc cctcgtagaa atcgaacatc gcgttcgcct tcgggcggta gttcaggctg
    20941 ctgccggtca tcgccaacgt ctggtcgtcg aaggtgatgc ccaggtccag ggtgcggcca
    21001 tcgccggcag tgcccttcag cgtgacctgc aacgacttga tgatgtccac gtgcatcacc
    21061 tgcgagtcgt ggaaggtctt gccggccatg ccgccgacga tctcgcgcac gtcgccggca
    21121 tcgaccttca tgccggtacc cagtttctcc agcttcttgt tggcatcggc gatcgcgtcc
    21181 tggccttcgc tcctgccgag cttctcgtcc agttgctcgc cgatgtcgtc ggcgaccttg
    21241 gtcgacagcg agcggaacga ctgcgtgccg ttgccgacat cggcctggat cgtgccctgc
    21301 acggcctggc cttccttcaa tggtgcatcg ccgttcgcaa gcgcacccgt atcggcggcg
    21361 tcctgcgcgg cctggtcgac catcgcctgc atccccgaag cgggtgtggc ggttgatgcc
    21421 gcatcgccgg ccttgccgca ggcagccagc atcaccgcga ccacggccaa cgccaaggtg
    21481 ttccgcacct gcttgttaac tcccctcatt gccgcactcc ttttcacgtt gttggtgtca
    21541 atccggacat tcgcacgcat gagcggtgcg tgggcgtccc gcgcagcgca tccatcgcgc
    21601 cctttccccc ggcaatcgcc aatgccggcc acgggcagtt acgcaggcgc gtggcgaatc
    21661 tggccatcgg atcttgcatc gagcccgccc cggtgcgggg cggttgctca gggcgtgcct
    21721 gcgcgaggac gcatggcgat gacagcacgc cggatatcgt tggcccggat gatgtagtgg
    21781 ccgaccaggc tgtcgccccg gcgttgcagt tcgagccggt gctccatccc gtcagcggtg
    21841 gggcgggaga ccagcacgat gctgtcgccc tcgatccaca cgcggtcgaa cgtggcaccg
    21901 aggccgatca gttcgcagtc ggcatcgccg gtgacgggca ggccggcgat cacatcgcag
    21961 aacggctggt acagcacgta gtcgccgctg ccggtggtgt cgccggcgtc cgcctggatc
    22021 gagaacgcca tcaacgcggc atcgtgcgga tcgtccgcgg cgaccagccg ccagttgcca
    22081 tgcaggcgtg cgggcgaggg catcgcgacg gcggtagtgt catcggccac gacgggcgcg
    22141 atggctgccg ggatgtcggc atcggccgcg gtgcaggccg atgccagcat ggcgatcagg
    22201 attgcaagga ggattcgcag gggcatcgca aactccgtcg gtggcgcaag cgtgggcgga
    22261 ggcaacggtt acgcagcggc gcacgcgatc cggacaagcc ggcatcggcc gatgctaagg
    22321 tgcaggcctt ccccgccgaa gtcatcgatc gcatgcacga agacgccgag ccgcagccgg
    22381 gcacgttggc tgcgaccgcc gccgaactag cgccgctgct gttggacgac atgcgccggc
    22441 gcgcgcgtcg cgaacgtcgc cgggtcaatg ccggcgaaac gatgcgcacc acggcgctgg
    22501 tgaatgaggc cttcctgcgc ctgcaacgcg gcggtgcctg ggaagacgag acccatttcc
    22561 tgcatgcggc ggcgatggcg atgcggcagg tgctggtgga ccatgcgcgt cgccggctgg
    22621 ccggaaagcg cggtggcggg cgcgtggatt cgctggaaga ccatccggac atcgagccgt
    22681 tctgggtcag cgacgaacgc ctggtcgagc tcgatgacgc cttgcaggaa ctgtcccggc
    22741 tcaacccgcg cctgcagcag gtagtcgaat gccgcttctt cgccggctac accgacgacg
    22801 aaaccgcgcg cgtgcttggc gtggccgagc gcacggtgcg ccgcgactgg atcaaggcgc
    22861 gcgcgtggtt gttccaggcg ctggagccgg tgccggactg agctgggcgc gcaggctggc
    22921 gctctgcgtg gcgatgcgct ggccggcagg gcccgcggct ttcgccatcg cgtcgaccgt
    22981 atccagcagc gccgcgaccg gcgcctgttc gttgcgagcc aggtggatgc gcgccaatgc
    23041 cagccgtggt gccgcaccgc ccggatggcc cgcgcccagg tgctggtccg cggcggcgag
    23101 cgcttcgcgc gcggtgactt ccgcacgctt ggcatcgccc agcgccagtt gcgcttcggc
    23161 gaccccgctc agggccgcga cgtggtgcat gctgcccgtg ccggcgaact gcgcgcccat
    23221 cgccgcggcc tcggccagca ccggcagcgc ctcgccggcg cgtccggtct gcagcagcaa
    23281 cttgccgtag ttgttgagca gggccgcggt cgccgccgat ggcccgtaca acgtccggcg
    23341 caaggtcacg gcctgttcca gcaggggcgc ggccacctcg ggtttgcctg cgctgagttc
    23401 cagcgaaccc cagttgttga gggtgttcag ggcatcggtg gattcgccca ggcccgccac
    23461 ccgccataca tcgcgcgcgg cacggaaggc ctcgcgtgac tcgtcgaggc gacccagccc
    23521 gaacagggcc acgcccaggt tgttctggaa caccccggtc tcgcggccat gcacgccatc
    23581 cagcgcgatg cgctgcgcca gcccgcgttg cagcagggcg atcgcgccat cggcatcacc
    23641 ggcctgctgg cggaggcgtg cttccagcat ctggctgtcg atcaaccgcg actgccagcg
    23701 cacgggatcg gtgttccaga aggctcgcgc ctgggcgagc agcgcggccg cgcgtttcgc
    23761 gtcgccgcca cgcagcgcaa cctgggcgag gtcgtagcgg cccacggcga ccagcgacgg
    23821 atcgacgatg cgggtgtcgg cgtcggccag ccgctgcaac agcggttccg cggcggggta
    23881 atcgttgagc aggaaataca gttcgcccag cgtatgcagc acgggcgcgc cttccgcggg
    23941 atcgcgggcg aaactgtcga cgacgcgttg cgcggcgtgg tcgagtacgt cgccggcggt
    24001 ggcatcgctg ccctgcatct cgccggcgct gcggaacatg tggaacaccg cctgctgcac
    24061 cgcttccagc cgcgcttttt cgcgcagcgc ttcatcgcgc tggcgaccgg cttcgcgggc
    24121 ctggaacagt gccgcgccaa gcccgacggc aagcgccagc acggtcacgc tggcacccgt
    24181 cgccgcccag cgatgcgtgt gcaggaagcg ccagaagcgg tagcgcggct cgccgtcgcg
    24241 ggccgcgacc ggcgcgccgc gcaatgcgcg cgcaaggtcg tcggcgaacg catccatgct
    24301 gcggtagcgc tgcgccgggt cgcgcgccag cgcctgcgcc aacaccgcgt cgaggtccag
    24361 caggaggcca cgctcggtcg ggatcgacac ggcggcggca acatcgcgaa ggcgcggcgg
    24421 gttcacctgt gcggcacgca tcgccagcaa cgcggtcggc ggttcgtccc gcgcatgcgg
    24481 tgcgtgcccg cacagcaggc gatacagcac cgctgccatc gcgtggatgt cggcggccac
    24541 gccgacgtcg ccgccggcca gttgttccgg tgcggcataa cccagcgaca gcggcgcgcg
    24601 ctcgccatcg gcctcctcgc cctggccgga caggctggcc acgccgaagt cgatcaggcg
    24661 cacccgtcca tcctggtcca ccagcaggtt ggagggcttg atgtcgcgat gcacgaccag
    24721 ccgcgcatgc gcatgcgcca ccgcctcgca ggcctgcagc accagcgcga tgcgttcgcg
    24781 cggcgaggca ttgcgttgct cgcaccactg gtggatcggc atgccgtcca cgtactccat
    24841 gaccatgtag ggctggccat cgtccagcaa gccgccgtcg atcatccggg cgatgccggg
    24901 atggtcgagc aaggccacga tcctgcgttc gttgtcgaag cgcgcccagt cgccggcatc
    24961 gacgcgggcg atgcgcttca gcgccgcgcg ctggccgtac tggccgtccg cgcgctcgac
    25021 ctcatggacc tcgcccatgc cgccgcgccc gagcaggccc acgatccgcc atgcgccggc
    25081 gctgtcgccg gcggccaggg tcgccggcga cagcgcatcc gcgtgggccg cgcgctccag
    25141 gaaatcgccg ctggcatcgg ctgcggcaag caggcgaagg acctcgtcga tgacggccgc
    25201 gggccggccg gtgctgcgca gccacgcctc gcgctgcggc gccggcatgt cccatgcggc
    25261 atcgaacagg gtctcgatgg cctgccactc gggggtcgaa gtcatgggtc ctggcgtccg
    25321 cgcgattcgc cacagcatgg cgcgcgcagt gcgcccgggt aaacccgcgt ggtgggcggc
    25381 tcgccgggat tgcgcggcgg cgaaacgccg ggcacaaaaa acccccgctt gcgcgagggt
    25441 tgtttgttga acctggtgcc cgggagagga ctcgaacctc cacgaagttg cccccgctag
    25501 cacctgaagc tagtgcgtct accaattccg ccacccgggc agggtgcagg gggcgtatgt
    25561 tgcagtgcga cttcgagctt gtcaacggga tttcatccga tgcggacagg gcctgcacgg
    25621 gcgcgttgta ccatcgccgc atgaccaaac ccccgagcaa gcgtggcccc ggccgcggcg
    25681 gcaagacacc cggcaagtcc ggcggcacac gaagcgaagg cgcaccgcag ggacggggtg
    25741 gcgcgaagaa aaccgccagc aacttgcccg ggtggttgcc cgatgccggc ctgatgcgtg
    25801 ccttcggtgg cgcaggtgcg gccaagcccg cggggcgtgc cgatcccaag cccgcgacga
    25861 agccggtgat gtccacgcgc cccggtcgcg agcgcgatcc gcacgcggcg cgcgaggcgc
    25921 agcgctacga acaacccatc gccagccggg agatgatcct gcagatcctg gccgccagcg
    25981 acggaccgct ggatgccgat gcgctggcgg cgaaattggc gctgaccgcc cccgaccggt
    26041 tcgccgcgct ggagaagcgc ctcaacgcga tgctgcgcga cggccagttg ctgcagaacc
    26101 gccgcggtgg tttcgccccg gccgcgcgca tggacctggt gcccggcgtg gtcatcgcca
    26161 acgagaacgg cttcggcttc ctgcggcctg acgcgggcgg tggcgatgac ctgttcctgc
    26221 cgccatacga gatgcgcaag gtcatgcatg gcgatcgcgt gctggccagc gtcaccggca
    26281 tggaccggcg cggccggcgc gaaggcacca tcgtcgaggt gctggaacgc cggctcaccc
    26341 gcctgatcgg ccgctacacc gaagaggccg gcatcggcta cgtggtgccg gacgacaagc
    26401 gcgtgcagcg caacgtgatg atcccggcga acggccgcaa cgacgcgcgc aacggccaac
    26461 tggtcgtcgc cgaaatcacc accccggcgg atgcgcatcg cccgccaatc ggcaacgtgc
    26521 tggcggtgct gggcgacaag ctcactgcga gccaggcggt cgaggccgcg atccacgggc
    26581 acgacatccc gcacgaattc ccgcccgaag tcctcgcgca ggcgaccgcg gtcccgctgc
    26641 aggtgcaggc tgccgacatc gccgggcgcg tcgacctgcg caacgtgccg ctggtcacca
    26701 tcgacggcga ggacgccaag gatttcgacg atgcggtgtg gtgcgagccg aacaaggacg
    26761 gcttccgcct gatcgtcggt atcgccgatg tctcgcacta cgtgcggccc ggcacgccgc
    26821 tggacgatga ggcgcaattg cgcgcgactt ccgtttattt ccccggcttc gtggtgccga
    26881 tgctgccgga gacgctcagc aacggcatct gttcgctgat gccgaaggtc gaccgcctgt
    26941 gcttcgtcgc cgacatgcag gtcggccgcg acggcatcgt cacaggtgcc aggttctacg
    27001 aagcggtgat gcattcgcat gcgcgcctga cctacaccga tgtctggaac gcggtgggcg
    27061 agggcattcc cgaggatgac cacgccgccg cgctggcgaa gatcggcggc ttgctgccgc
    27121 agatccggca actgcaccag ctctacaagg tgctcgacaa ggcgcggcag aagcgcggcg
    27181 cgatcgagtt cgagagcagc gaggtgcgct tcgtgctgga cgcgcagggc gcggtcagcc
    27241 aggccggcat gctccagcgc aacgatgcgc acaagctgat cgaggaatgc atgatcgcgg
    27301 ccaatgtgca ggccgcgctg tacctgctgc agtccggcgt gccggcgccg tatcgggacc
    27361 acgacaagcc accggagtcc aagtacgcgg acctggagga attcctcaag gaattcatgc
    27421 tgcgcatgcc gccatgggcc aaggtcaagc cgaaggattt ccgcgcgatg ctggaaaaga
    27481 tccgcgaacg tcccgacgcc acgctgctgg agtcggtgat cctgcgcagc cagtcgctgg
    27541 cggtgtacgc gccggagaac atcggccact tcggcctggc gctggaggcg tacgcgcact
    27601 tcacctcgcc gatccgccgc tatcccgacc tgctggtgca ccgcgcgatc aagcatgccc
    27661 tggccggcgg caagcccgag acctacacct attcgccgca cgagatggcg gcgctgtcgc
    27721 tgcagtgctc ggagcgctcg cgtcgcgccg acgaggccca gcgcgaagtc gacgagcgct
    27781 accgcgcggc gtggatggaa ggccacatcg gcaaggaatt cgacggcacc atcagcggcg
    27841 tgaccagctt cggcctgttc gtggagctgg acgattccaa ggtgaacggt ttggtccacg
    27901 tgacccagct gccgcgtgat ttctaccact tcgatgctgt gcgcaagacc ctggccgggg
    27961 agcgtcgcgg ccacgaatac cggctgggcg accgcgtgcg catcgtggtg ctgaaggcca
    28021 gcgtcgaaga ccgcaagatc gacttccgcc tggtcggcga cgaaggcgcc gctgcagcac
    28081 cgatgccgat gccggaacgc ggcaagccga agaagcgcac gaagcagaag tactgatctt
    28141 ctacttcctc ccttgcgaat gcaggggagg gttggggtgg ggttgccttt gttggatcga
    28201 cctaaaagca ccccctccca acctccccct gcattcgcaa ggggaggagc tgaggcaaaa
    28261 tagcgccatg agcaagcaag cccaatggat cgtcggcctc aacgcggtgg cttccgcgct
    28321 ggaacacgac gccgaacacg tgcgcgaggt gctgctggaa gccggcggca agaacccgcg
    28381 catcgccgag atcgaacagg aagcgcggcg caaggagatc gatgtgcgcc gcgtcgccca
    28441 gaacgcgctg gatggcgtcg ccggcggcgt gcgccaccag ggcgcggccg cgcgctatgc
    28501 ggcggcgaag acctgggacg agcatgaact ggtcggactg gtcgaagcgg ccggcggcaa
    28561 ggccctggtg ctggtgctgg atggcgtgca ggacccgcac aacctgggcg cctgcctgcg
    28621 cagtgccgct gccgcgggcg cgaccgcggt ggtgttcccg aaggacaagg cggtgcaggt
    28681 caatgccacc gtgcgcaaga cttcggccgg cgcggcggat accttgccga tcttcagcgt
    28741 gaccaatctg tcgcgcacgc tgcgcgacct gcagaaggct ggcgtgtgga tctatggctt
    28801 cgccggcgag gccacggctt cgttctacgc actggacctg aagggcaacg tcgcgctggt
    28861 gttgggcagc gagggcgatg gcctgcgccg gctcacccgc gagaactgcg accagctggt
    28921 gaagatcccg atgccgggcg agttcgagag cctgaatgtc tccgtggcca ccggcatcgc
    28981 cttgttcgaa gccgtgcggc agcggcaacc gtaatcaagc gcgcaagcgc aaggggagca
    29041 cgatggcgct ggaattggcg tggtacgact gggttggcct gctgggcacc gtgaccatcc
    29101 tgctgggatt cttcctgctg caagccggtc gtctcgcggg cacgggcctg gtctatcagt
    29161 tgctcaacct gttcggcgca gccgggatcc tggtgtcgtt gatgggcaag ttcaacttgt
    29221 ccgtgttcct gctggaagca gcgtggatgg cagtgagcct gtacgggatc gtgcggtcga
    29281 tcaaggcgaa acgcacccaa gcgcgatgag tcgacgggcg tcatgccggt ggcgagcgca
    29341 gggacaatcg gcgccacgga tagcgccgat tagcctacat ggacgtactt gcggcgtgtc
    29401 ccaagccgct gccggcgtgg cgaccggcga cggacttcaa cgtggccacg cattcgcgat
    29461 gatgcagaac agctgcgcag tgcgttctgc gtcgtagacc gcggaatgcg cctcgcgctg
    29521 gtcccattca aggccagccg catgcaccgc gcgtgcgagc accgtctgtc cgtaggccat
    29581 gccggccagg ctcaccgtgt cgaacacgct gaagggatgg aacgggttgc gcttgtgctc
    29641 gacgcgggcg atcgccgcgt tgaggaaatt gaggtcgaag tgcgcgttgt ggccgaccag
    29701 gatcgcgcgc tggcagccgt ggcgcttcgc cgccgcgcgc accggcatga acaccttgtc
    29761 caggccttcg cgctccggta ccgcgccgcg tagcggatgg tcgaccttga tcccggtgac
    29821 ttccagcgac ttcgggtcga tgctggtgcc gggcgcaggc tggatgtgcg cgctggcgac
    29881 ttcgccgggc acgaaacgcc cgtcttcgtc cagctcgatc ggcaccgccg cgatttccag
    29941 cagcgcgtgc cggttccagt cgaagccacc ggtctccacg tccaccacga ccggcaggta
    30001 gccgcggaaa cgctccacca tcggcttgaa caggggggcg tcggtgggga tcgcgtcggc
    30061 atcggtcatc gcgacaggtt agcagcgtgt tgacgaggcg ggcgagcctg ctagggtgcg
    30121 ctatccatcg aaccggggcg ggcgatgcgg aagcagacac ggatctggcg cgtgctggcg
    30181 gtggcctgcc tgctgtccgg catgccagtg ctggcagcat cgaccgatcc ggtgctacag
    30241 gtgaacacct tcatcggcac ccaggaggag ggcaacacct tccccggtgc atccgccccg
    30301 ttcgggatga tccaggtcag cccgatcacc gagcactacg ccggctatcg ctatgacgac
    30361 acgaggatcc gaggcttcgg tcattcgttc gtgtccggcg cgggttgctg ggagcagggc
    30421 ggactggtcg cgaccttgcc cgtgaccggc cgcatcggcc cgggcggcgc tttcgacaca
    30481 agcgacgcga aggcaaagcc cttcgactac aagacgtatg ccgcggccta cacgcacgcg
    30541 ggcgaggttg gccaggctgg ctactacaag gtccggctga cggactacaa cggcatcgag
    30601 gtcgaaacca cggcgctgac ccgcgccgcg gcggagcgct acaccttcgc tgtcggtgaa
    30661 aaggtcggcc acgtgctggt caacgtcggc caggccaacg agaagcacga agtggtcggc
    30721 agcgtaatcg acgtcgtcgg cgatcgcgcg gtggaaggca agctggtcac caggagcttc
    30781 tgcggcggca agcagtacac cacgtggttc cgcatcgaat tcgaccggcc gttccaggcc
    30841 ttcggtacct gggatcgcga gggcggtgtt ccgggtggcc gccacagcgt gggcacgcaa
    30901 tgggatgtgg ccaacggtgc gtggctgagc ttcgacttgg ccggctctcg cagcgtgact
    30961 gcgaccagtg cgatctcgca tgtggatgcc gacggcgcgc ggcgcaacct cgatgccgaa
    31021 ggcaagcacg cgggcaagtt gcttgcgttc gatgccatgc gcgcgcaggc gcaggccgca
    31081 tggcgcaagg aactcggcgt ggtacgcatc gacggcgccc cggacgacga ccgcgtggtg
    31141 ttcgataccg cgctgtacca cgccttcctg cagccgatga ccggcagcga tgccgacggg
    31201 cgctatcgcg gctacgacga ggccatccac gtggccgagg gttggacgta ccacgagtat
    31261 ttctcattgt gggataccta ccgcacgcag aaccagttgc tggcgatcct caagccggcg
    31321 cgcgcgcgcg acatcgcgcg ctcgatgctg gcgatccacg aacagatggg ctggctgccg
    31381 cgctggggat acgccaactt cgacaccaat atcatgaccg gcgatccggt gacgccgtgg
    31441 ctggtcgacc tgtggcgctt cggtgcgctg cagggccgcg aacgcgaggc tttgcaggcg
    31501 ctgtggcaga acgcgaatgg ccgtccgccg gcgatgtcgc gcgcgcaggg ccgcgccggc
    31561 aacgatacgt accttgcgca aggcttcgtg ccctacgacc gcgcctatcc gtccaagggc
    31621 atggacgtgg accagcacca cggcggttcg gccacgctgg aatatgcgct gggcgattgc
    31681 gcgctgtcgc agatggccgg tgcgttgggt gaaaccgcca tcgccgccac gctgcgcgaa
    31741 cgtggccgaa actggcgcaa ggcctgggat gccgacgtcg tcgacaagga gctcgcctac
    31801 cgcggtttcc cgcggccgcg catcgaaggc ggcgcgttct acgtgccggc gcacggcggc
    31861 tacgacccgc gttccaacca cgggttccac gagggcaccg cctggcaata ccaatggttg
    31921 gtgcagcagg acgtgcccgg cctggtggcg gcgatgggcg gcatcgagca ggcgcgcaac
    31981 cggctggatg cgttcttcgc ccaggacgaa cttgcgaagg acgtgcgggg cggggcgcgc
    32041 acgcattggg tggtcgggcc gtacagctac tacaaccagt tccgctacaa cccgaacaac
    32101 gagcccgacc tgcatgcgcc gtggatgtac acgctgctgg gccagccgtg gaagaccacg
    32161 accgtcgtgc gcgccgcgca ggccctgttc accaacgcac ccaacggcgt caccggcaat
    32221 gacgacctcg gcacgatgtc ggcgtggtac gtgttcagtg cgatcggcct gtatccggcg
    32281 gtgccgggca ccgggcagtt gctgctgcat gcgccgcgct tcccgcgcgt ggacctggcg
    32341 ctgggcgagg gccacgtgct gaccatccgt gcggccggcg cgaatgccgg cgccgcgcag
    32401 gcgattgcag tggtcggcat cgacggcaag ccgcatgcgc aagcgtgggt ggattgggcg
    32461 acactgcgcg atggcggcag catcgacttc acgcttgctg cgcgcgccga tccgaacggc
    32521 tggggcacgc gcccgcagga cctgcccccg tcaccctgcg ccgccccatg acccgacgcg
    32581 aggcccgcat gcgccccatc ctctgtttcg gcgaagccct gatcgacttc ctcgcgcgtc
    32641 ccggcgacac cccggacgcg ccgcgcgcgt tcctgcaatt cgcgggcggt gcgccggcga
    32701 atgtcgcggt ttccgtggcg cggctcggcg gcaacgcaca gttcatcggc atgctgggcc
    32761 gggacatgtt cggcgacttc ctgctcgaca gcctgcgcga tgccggcgtc ggcaccgacg
    32821 gcatcgtccg taccagcgat gcgaagaccg cactcgcatt cgttgccctg gatgccgatg
    32881 gcgaacgcag tttcagtttc catcgaccgc ctgccgcgga tcttctattc cgcgacgagc
    32941 acttcatccc cgcgatgttc gaaggcgcag ccgcgttgca tgcctgttcc aacagcctca
    33001 ccgagcccgc catcgccgag gcgacctacg cgggtttgcg tcgcgcccgc actgccggtg
    33061 cgctggccag cgtggatctc aacctgcgtc aggtgctgtg ggcagcggag gtggatccga
    33121 cgccctggct gtggcggctg ctggacgaag ccgtgctggt gaaactggcg cgcaacgagc
    33181 tggactacct cgccgaacca ttgggcagcg aggatgccgt gttcgaccgc ctgttcgccg
    33241 gccacgcgcg gctggtgctg gtcaccgacg gcgctgccga catgcactgg cgcacgcgcg
    33301 cggcgcgcgg cacggtcgcc ggcttccgcg tgcgctccat cgataccact gcggccggcg
    33361 atgccttcgt cggcggcctg ctgttcgcgc ttgtgcaaca aggtgtcgat gccccccgct
    33421 tcgatgcctt catcaatgat gaagccgcga tccgcgacgc gatgcgcttc ggtgccgcct
    33481 gcggcgcgtt ggccgtcacc cgccatggcg cctttgccgc catgcctacc catgcagacg
    33541 tgcagcgctt gctgcaggac gaggacaccc atggaccagc ctgacttccg ttcgcccgaa
    33601 ttcctgcgcg cgcacatcgc gcagaccatg gcgttctacc acccgcgctg catcgatccg
    33661 gccggcgggt tcttccacta cttcaaggac gacggcacgg tctacgacgc ccgccatcgc
    33721 cacctggtga gcagcacgcg cttcgtcttc aactatgcga tggcctggcg cgaattcggc
    33781 aacaccgaat atcgcgaggc gatgttgcat ggcctggaat acctgcgccg ggtgcacctg
    33841 gacaccgaaa ccggcggcta cgcgtggacg attcgcgacg gcgtggccga agaccgcagc
    33901 aatcattgct atggcctggc gttcgtgctg ctggcgtatt cgcatgcgcg gatggccggc
    33961 gtggccgatg cggccgactg gatggaagaa atctggatgc tgctggaggc gcgcttctgg
    34021 gaggccgaac acgggctcta ccgggacgag gccgatgccg actggaactt cagcgactac
    34081 cgtggccaga acgccaacat gcacatgtgc gaggcgatgc tcgcggcgta cgaggcaagc
    34141 ggcgacaaac gctggctgga tcgcgcgctg ctgctggccg gcaacatgac ccgccggcag
    34201 gcggcgcagg ccggcggcct ggtctgggag cactacgacc gcgactggaa cgtggactgg
    34261 aactaccacc tcgacaatcc caagcacctg ttccgaccgt ggggtttcca gcccgggcac
    34321 cagaccgagt gggccaagct gctgctgatc ctggatcgcc acgtcgccac cgattggttg
    34381 gcaccgatgg cgcgccacct gttcgatacc gcatcggcgc gatcgtggga caccgaacgc
    34441 ggcggcatgt attacggctt cgcgccggaa tcgcgccggc aaccgggcat ggacggcgcg
    34501 ccgatcggcg gcggaagctt cgtctgcgat gacgacaagt atttctgggt gcaggcggaa
    34561 tcgctggctg cggctgcgtt gctggcggcg cgcttcgacg accaggaata ctgggactgg
    34621 tacgaacgcc tgtgggcgta tgcgtgggcg cacatggtcg accaccagca cggcgcgtgg
    34681 ttccgcatcc tcgatgccga caaccgcaaa tacgacgaca acaagagtcc ggccggcaag
    34741 accgactatc acaccatggg cgcggcctac gaagtgatga acctgctcag gccggtgcaa
    34801 tgacgcgtgg caggttccgc atggtggcat ctgccgccat gctttcgatg ctggcgatgg
    34861 cacccgcgat ggcgcagcag gccaccgagt acgtcgctcc gccggcaccg acctatgcgc
    34921 aacccatgga cgacgccgag tccgcgcgca tggcggcgcg cgtgcgcgag gaaacccggc
    34981 atgcgtggca gggttacatg cgatacgcca agggccacga cgcactgaag ccgctgagca
    35041 ggaagccgca cgactggtat ccgcaatccc tgctgatgac gccggtggat gcgctggata
    35101 cgctgctgtt gatggggttg gacacggaag cggcggaagc gcgcgaactc atcgccaccc
    35161 agttgtcgtt cgaccatgac atgccggtgc agaacttcga ggtggtcatc cgcctgctgg
    35221 gcggcctgct gtccgcgtac cagctgaccg gcgacgagcg actgctcgcc ctcgcggatg
    35281 acctcggcac gcgcctgctg ccggtgttcg aatcgccgac cggcctgcca tatacccacg
    35341 tcaacctgcg caccggcgcg gtcaagggca aggtcagcaa ccccgccgaa accggcacgc
    35401 tgctgttgga actcggcacc ttgtccaagc tcaccggcaa gccgatctac tacgacaagg
    35461 ccaagcgcgc gttggtggcg acgttcgagc ggcgctcgac ggtcggcttg gtcggcagcg
    35521 gcatcgatgt cgagacaggc gaatggaccg atcgcgacag ccacgtcggt ggcggcatcg
    35581 attcgtacta cgagtacctg ctgaagtgct ggaagctgtt cggcgaccag gactgcaaac
    35641 gcatgtggga ggagagcgtt ggtccgatgc accgctatct cgccgacgag gtgcgcgatg
    35701 gcgaactctg gtatggccac gccgacatgg acagcggcgt tcgcacgacc agcgtgtacg
    35761 gcgcgctgga tgcattcctg cctgcggtgc tggcgctggg cggcgacctc gaccgcgcca
    35821 agcgcctgca gcagtccggc caggcgatgt ggcggttgca cggcatcgag ccagaggcga
    35881 tcgactatcg caagatcgaa gtgctctccg gcggctaccc gttgcgcccg gagatcatcg
    35941 agtcggccta ctacctgcat cacttcaccc gcgacccgcg ctggcgcgcg atgggccgcg
    36001 agttcttcga cgacttcgtc cgccacaccc gcaccgaggc cggctacgcc gcgatcaagg
    36061 acgtgcgcac gatggaacag cgggactcga tggagagctt cgtcctggcc gagactttca
    36121 agtactggtg gctgctgttc tcgccgccgt cggcgctgga cttcgatggc gtggtgttca
    36181 acaccgaagc gcatccgttg cggaagacct ggaaggattg agtatcagcc ccgctgccgt
    36241 taactcgcca tctcgcccag gccagtccaa tttcctcatg aggcccgcaa gcgaggaggg
    36301 ttgatgggtg tagctgcttc cgaccaaagc ggacactccg ctcatcacgt ggggtctgca
    36361 ctcccagaag ggcagccaat cgcctgctga ttgcgtgccc cgcaggcctt tgcgatgccc
    36421 aacaacaggg tttgggccgt ggcatcccga ggcgacttga ttccgatcaa aacggatgtg
    36481 agatggatcg tgcgaagctg ggtcgcacct tcgccgagag gaggccgccc gacgacttgc
    36541 cgcgaacggg cccaatgtct tgcaggtggc aggcctgacg ttgtttgccg gaatcgtcct
    36601 gggaatcctc gtcgagccat cgtctttccc gccgtcaccg ctggcggcca tcgtgacctg
    36661 ctatttgacc cgctgttcca acggccaaac atgagaagac cgcatgcatc cccagggtat
    36721 cggcgccgcc ggtgaagcca ccggatcctg tcatctcgtc catgtgggtg atcgcaccat
    36781 cttgctcgac tgtggcctgg tccagggcgg caggagcgcc gacaagcgca accgcgaacc
    36841 gtttcccttt gacgcgtcca cgatcgatgc ggcggtcctg ggccatgcgc atatcgatcg
    36901 caaggaattc gccacgacag cggaggtcat gcagccggga gagcgcccca acctcgacgg
    36961 cagcgctcgc gtgcgggtgt cgcgatgagc gcggccaagg acagtgttgc gggcgatgtc
    37021 gtcgtggccg cgcacgaccg actggtcgca acccgcaccg gcatcgacac ccgtggcgag
    37081 ccggtggtct atctgcgcaa tgacgctgcg atcgcacgat cggagggatt cgcagcgctc
    37141 gcgcgcatcc gcgtgcgtgc caatggacat gcgatcgatg cgaccctgtt cactgtctcc
    37201 gggccgcgct gcatgctcgc acccggccag gtggggttgt ccgaggcggc gttctcggcg
    37261 ctggacgtcg aggaaggcgc ggcactcacc ttgagccatg cgccggccgt cgagtcgctg
    37321 gggtttttgc gggccaaggt ctatggccaa cggttggatg ggcaggcgtt caacgccatc
    37381 atccgcgaca tcgtgggcgg tcgttacatg ggcgtgcacc tggccgcgtt cattaccgcc
    37441 tgttcaggtg cacgcctgag cccggacgaa atcgtggacc tgactggcgc gatggtcgac
    37501 gtgggccaaa ggctgcagtg gccgggcaag tgcatcgtcg acaagcattg cgtcggcggc
    37561 ctgcccggca atcgcaccac tccgatcgtg gtggcgatcg ttgccgcgca tggactgacc
    37621 atgcccaaga cttcgtcgcg ggcgatcacc tcgccagcgg gaagcgccga caccatggag
    37681 accctggcac cggtcgacct ggacctggcg acgatgcggc gcgtcgtcga acgcgaaggc
    37741 gcctgcctcg cctggggagg ctcggtcagc ctgagtcccg ccgacgacat cctgatccgg
    37801 gtcgaacgtg cgctcgacat cgacagcgaa ggccagttgg tggcgtccgt gatgtccaag
    37861 aagatcgcgg ccgggtccac cgacatcctg atcgatatcc cggtgggccc caccgcgaag
    37921 gtgcgcagcc gcgcggccgc cgatgcattg gaagcacagt tccagtatgt cgcccaggcc
    37981 ttcggctgca ggatccaggt gttgcagacc gacggcagcc agcccatcgg caggggtgtc
    38041 ggtcccgcgc tggaggcgcg cgacgtgctg gcggtcctga aatgcagtgc ggacgcgcct
    38101 gccgacctgc gtgagcgcgc ggtgttgctg gccggcacct tgctggagag cgtgggtgca
    38161 gtggctgccg ggcagggcca gttcgaggca tccgccaccc tggacgacgg ccgcgcgtgg
    38221 cggaaattcc aggccatctg tgaagcccag ggcggcatgc gcgtgccacc ggtggcgcgt
    38281 catcggcagg acgtgttcgc gccccgctcc ggggtggtgc ggcgaatcga caaccggttg
    38341 atcgcgcgcg ccgcaaaatt ggccggtgcg ccaggcgaca aggccgcagg gcttttcctg
    38401 cacgtgaagc tgggcgatcg cgtgcgcgaa ggcgatcccc tgctgacgct gcatgcggaa
    38461 agcccgggtg agcttgcgta tgcgctggat tacgcgcgta cccagggcca ggcctttgaa
    38521 ataggggagg actgatgcga ccgatgctgt tcaacctggg catcggcgat ggcctgttcg
    38581 attcgctgtg cggggcgctc gacgcgcagg ccgcgaccct cgagatccgc agcttcccgg
    38641 acggggaaag ctacgtgcgt gccttggacg attgcgaagg gcgcgtggtc acgctggcct
    38701 gcagcctcga tcgcccgaac gccaaaatcc tgccgctgct gcttgccgcg gagaccttgc
    38761 gcgacctcgg tgccgcgcag gttggcctgg ccgcgcccta cctggcctac atgcgccagg
    38821 acacgcgctt caaggtgggc gagggcatta cctcgcgtta ctttgcggac ctcttgtcgc
    38881 ggcacttcga ttggctggcg accgtcgatc cacacctgca ccgcttccat gcgatggacg
    38941 agatttaccg cgtgccagca cgggtggtcc acgccgcgcc aacgctggcg cagtggatcc
    39001 gccagcatgt cgagcagcca gtgctgatcg ggccggacag cgaaagccgg caatgggtgt
    39061 ccgaggtggc ggccggcgca ggcgcgccgt tcttgatcct ggacaagcag cgcagcggcg
    39121 accgcgacgt gcgcgtgtcg gtgcctgacc tcgcacggca catggatcgt gtaccggtga
    39181 tcatcgacga catcgtttcg accggacgca ccgccatcgc cgcggtctcg cacctggcga
    39241 cactgggcgc gccgccgccg gtctgcgcct tcgtgcatgc ggtgtttgcc gatgatgccg
    39301 aagcgatgct gaaagcggcg ggtgcctcgc gcgtggtgag ctgcaacacc atcgagcacc
    39361 ccagcaatgc gatggatatg tcggcacccc tggcgcaggc ctgccgggac ctgatggcga
    39421 catcgcccga gcgtcgccga tgaagacgcc cgcagccggc gtggacaacc ccaccgcacg
    39481 tggcggagac gcggcatgcc accagcccat gaactgaccg gattgttggc agcgctcggg
    39541 atcggcttgc tgatcggcat cgagcgcgaa cgcagcaagg gcgaaggatc gacgcgcggc
    39601 agtgcgggcg tacgcacgtt ctgcctgctg gcgctggccg gtgcggtcgc ggcattgctg
    39661 gggggcgtcg gtatcgcggt agccgggggc ttcgtggtgc tgactgcctt ggccagctat
    39721 cggcagacgc gagcgaatga tccgggactg acatcggaag tgtcgatgtt gctggtgttc
    39781 ctgctcggca tgctggcgat gcaagaggct gcgctggcgg ccggggttgg cgtggtcgtg
    39841 gcgatcctgc tggcctccaa gacgtggctg caccgcctgg tgcgccaact gctgagcacg
    39901 caggagttgc atgacggcct gttgctggcg ggctccgccg cgatcgtgtt gcccttgctg
    39961 cctgaccgcg cgattgatcc ctggcaggtt ctgaacccgc gcaagttgtg gctgttggcg
    40021 gtgctggtga tggtgatcaa taccgccggc catgtggccc tgcgcatgtt cggtgcgcgc
    40081 ctgggattga tgctggcggg cttcgccggt ggcttcgtgt cgagtaccgc gaccatcgcg
    40141 agcatgggct ccaagtcccg tgaactgcct gcgctcgcct ccacctgcgc aacggccggg
    40201 gtgttgtcga acctgagcac ggtggtgcaa ctggcgttgg tgaccgggat gttgtcgcca
    40261 ccattgctga agcaactggc gttgccattg ctggctgccg ctgcagttgc gctggggttt
    40321 tcgctgcttg caggctggca cggcgcccgc gccgacgaac ctgaagtcag caccctgacc
    40381 gggcgtgcat tcaagccgct gcacgtgctg atgttcgtgg ccatcgtggc cgcggtgatg
    40441 ctgttctcgg cgatcatccg cgatgccgtt ggaacgaggg cgctggtgtg gacgctggcc
    40501 acctcgggcc tcgccgatgt gcatgccgcc gccgcttcgg ccgcacagtt tgtcggtctt
    40561 gggcggatgg acctgcggtc cgcgaccgtg gcgatcctgt ttgcattcgc cgccaattcc
    40621 gccggcaagc tggtggtcgc gttcgtaacg ggcggacatc gctatgggtt gcgcttgttg
    40681 ccgggcatag tcggcatgct ggccgcgttc gcggtggtcg tcgtctttgg ttggtgaggt
    40741 tgcgatatct ggagaatcgg cgtggctgag gccacgcatg tcagttggtc atggttctct
    40801 cgtcaagcgc aattggatgt ggtgcgatga gaggcaaacg tccgctcctg gccgaggctg
    40861 tgt
//
LOCUS       DAOOCX010000005        36256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_865_length_36256_cov_8.936411, whole genome shotgun sequence.
ACCESSION   DAOOCX010000005 DAOOCX010000000
VERSION     DAOOCX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 36256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36256
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_865_length_36256_cov_8.936411"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PK399_00920"
     CDS             complement(<1..770)
                     /locus_tag="PK399_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPW11722.1"
                     /translation="MDNALRERFGGIDRLYGSGALARFNASHVTVIGIGGVGSWAVEA
                     LARSGIGKLTLIDADDLCVSNTNRQLPALEGQYGRAKVEAMGERLRAINPGIELDLRP
                     QFLTPTNLGDMLDAPGELVLDACDSFRSKVEAIAYCRRRKQPIVTVGSAGGRSDVTQI
                     RVRDLSRTEHDAMLSLIRKKLRGEFNFPKNHDRYFGVPAIYSLENVRYPQADGSVCGI
                     RPQLGKDEALKLDCGAGLGAATHVTGAFAFAAVGKVLE"
     gene            1000..1491
                     /gene="phaR"
                     /locus_tag="PK399_00925"
     CDS             1000..1491
                     /gene="phaR"
                     /locus_tag="PK399_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HPW11723.1"
                     /translation="MTATRIIKKYPNRRLYDTEISSYITIEDVRQLIIDGETFEVRDA
                     KTGEDLTRQVLLQIIAEHEQDGQPMLSTQLLSHLIRFYGDSLQGFMGNYLERSMQVFL
                     DQQQQFRQQMGGLMGQTPWTMLNQLTERNMDVWKDFQKNLVGGMGSPTGSAQRDRDPR
                     KPR"
     gene            complement(1575..2480)
                     /locus_tag="PK399_00930"
     CDS             complement(1575..2480)
                     /locus_tag="PK399_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPW11724.1"
                     /translation="MNQDPMGNGYNRQPRRGLGGGGLRLLVVGAMLLYGAYYYFSNRT
                     VDPYTGEQVLIDKTLDAEQESAMGLQAYREILSQERPVDPNSDIARQISGIAARLIDK
                     VDDVERDLAAERGIEARNFAAGFDWTVNVIQSDQANAFALPGGKMAVYTGLVPVAKNQ
                     DGMAVVMGHEIAHALMRHGAQRMARGKLEQIGQMAGAAGGMDAGTMQTVMSAYGVTSA
                     LPYARGHETQADEVGLMLAAAACFDPQEAVPLWERMAQASGGQGQPEFASTHPSPETR
                     IQQLQALMPKALAFRDKYCGTTGRT"
     gene            complement(2533..4365)
                     /locus_tag="PK399_00935"
     CDS             complement(2533..4365)
                     /locus_tag="PK399_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPW11725.1"
                     /translation="MRAGWTVIALFVPLALAACGPETPVAPTPQAAQPELPAGSVSIS
                     GDDALAGVLSWSLPAVQVDDPAAARKAAQRALEQGDLFETSASAIPILIALQRQYPGE
                     EGDAKLLERAQAALIGQANAALDAADEDLAALRFAQRHATVLRTLWPDAVRAKAYLQA
                     VDAAEQARERSLAGERALLAGALDTPGGALDAFRDALRLRPGQMRAHLGIGAVESALI
                     RSAEAAAKQGDFDTARADLARAQRVRQHPQTIPAAAARIEVMRAERIRRLRDEGLIAL
                     GEPDGLRVAQERLADMLLIARAGDPASMELRQRIDLVGHYGLFRPGQVFTDGLDDGSR
                     GPGLVVVPHGAFHMGAPDDEADASKDERPRHLVRLERGFAMTRHEITVGEFGRFVAAT
                     GYEARATERGHSLAYDVRSGNFVRGSGIDWRSGYDGRPAAADMPVIHVTPRDAEAYAA
                     WLSEQAGAHYRLPSEAEFEYALRAGSTTRYPWGNGSPPDGVENLTGGRDVSPQGRRWS
                     NAFVGYGDGWWGPAPVGSFAANAFRLHDMAGNVSEWVADCWHQGYRRAPATGAAWINP
                     GCRNRMYRGGSWASAPAQVRSAWRASGGTDATNARVGFRLVRQI"
     gene            4457..5551
                     /gene="rnd"
                     /locus_tag="PK399_00940"
     CDS             4457..5551
                     /gene="rnd"
                     /locus_tag="PK399_00940"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005414035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HPW11726.1"
                     /translation="MSQWIDTPDALRARLAAPPASIGLDTEFIRERTYWPQLALVQIA
                     LGDGGDVLLVDPLVPGMAEALVPLLVDPGVLKLMHSPSEDLVAFQHTCGVLPAPLFDT
                     QAAAALCGIGGGMSYQKLVLAVTGVALEKGETRSDWLRRPLSASQLEYAADDVRHLHA
                     MHASLQQQLNTLGRSSWLAEDSARQLANAANEEGDRWPHLVMRSAQFLDAPAQRRLLR
                     LLRWREAHARRSDRPRSWILDNELAVTLARANPADPAALQAQLDATPKAPKSLRDPIW
                     QALTTPLADEDEAPLARGEDRDKKQLRTMQDAVLAVAGELGVPEGVLASRKLLEALQD
                     GGGWSGPLAGWRRGVLEARLQPLLAGTTTA"
     gene            5603..6907
                     /locus_tag="PK399_00945"
     CDS             5603..6907
                     /locus_tag="PK399_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011409596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPW11727.1"
                     /translation="MRHSLLVAALLALLPCSALAADAQMEALATKVDGKVQAWRRDIH
                     QHPELGNREVRTAKLVAEHLRALGFDDVRTGIATTGVTAVLRGGQPGPRIALRADMDA
                     LPVTERSDLPFASKATSTFRGEPVGVMHACGHDAHVGILLGVAEALASIKAELPGEIL
                     FVFQPAEEGPPDGEEGGAEEMLAQGIFDRFKPQAVFGLHVFSTLNAGQVGWRSGPAMA
                     ASDRFNIVVQGRQTHGSRPWGGVDPIVASADLIGTAQTIVSRRQDISRLPVVLSFGAI
                     KGGIRYNIIPDAVELVGTIRTFDEGMRQAVFADLKNVAESVAKAHGATAVVKVPDTKG
                     NPVTANDPALTARVVPSLRKAVGDANVVEMGLVMGAEDFSFYAREVPGFFFFVGATPR
                     GQDAIKAPSNHSPEFFLDEEALKVGTRAMLQVVLDHQHGTGG"
     gene            6991..7066
                     /locus_tag="PK399_00950"
     tRNA            6991..7066
                     /locus_tag="PK399_00950"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7024..7026,aa:Ala,seq:cgc)
     gene            complement(7132..8121)
                     /locus_tag="PK399_00955"
     CDS             complement(7132..8121)
                     /locus_tag="PK399_00955"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW11728.1"
                     /translation="MPDAAIERLMQRYEGDVPGASLLVLRDGEARVHRGYGRSDLEHG
                     IEAGAATNYRLASVSKQFTAAAILLLAQDGSLGIDDPVKRWLPSLPPATAGITLRHLL
                     THTSGLLDYEDLMAEPYEGQIRDAGVLALLERENRLAFPAGSAYRYSNSGYALLALVV
                     ERASGMDFPAFLRSHIFLPLGMHDTVAYVAGSADVSNRAWGYSDVDGQWQRTDQSATS
                     AVLGDGGIYSNTHDLARWDAALYDDRLLNDASRALAFGKQVEVTGESAATHYGFGWRI
                     TGDTLWHSGETIGFRNVIVRWPAQRLTVILLSNRNDPAPYRTALDIGALFLRD"
     gene            complement(8140..8793)
                     /locus_tag="PK399_00960"
                     /pseudo
     CDS             complement(8140..8793)
                     /locus_tag="PK399_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            complement(8843..10375)
                     /locus_tag="PK399_00965"
     CDS             complement(8843..10375)
                     /locus_tag="PK399_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPW11729.1"
                     /translation="MPTAHAAAPIEGPTPQAIDAAVRATIDRYQLPGIAVGVIEDGKV
                     VYARGFGETVAGSGDPVTTKTLFKIASNSKAMTAAVLARLVQQGKLRWDDPVVKHLPQ
                     FAMHDPWVTRHMTVSDLLVHNSGLPEGGGDLMLWPEPNLFSRDDIVRGLRHIKPAYGF
                     RAGYAYDNLLYVVAGEVAAAAGGASYEDLVRRELFQPLGLSGCRVGEFALADAASVAQ
                     PHTRNGTRNEAMRRDGAIVPAIASAPAGGIRCDLDDMLAWASNWLVPTDAQKQWLGDA
                     QRAELWKARTPMPISAQRRRWDNTHYYAYAYGFRLADVDGEWTVSHTGTLGGMYSMMM
                     LLPDRRSGFVFMINGDGSNARTVLGQVLLKLFTAPEKSLGVAGYADEVARPAAPNGAA
                     APKPPLPDASKRSPVAPGQLTGWLGTWRDPWFGEVRLCAVDGAVEWRSLKSPKMHGRV
                     GELDGRYLLHWDDPAVDLDAFLAFSVGSSGPRMRMAKIDPAGDFSSDYEDLDFVRVRG
                     CD"
     gene            complement(10621..13602)
                     /locus_tag="PK399_00970"
     CDS             complement(10621..13602)
                     /locus_tag="PK399_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW11730.1"
                     /translation="MSTAPSRLRKSALSIAMGLCLASLAAAPAFAQSATGAVAGRANP
                     GEQVTVVNSATGASRSVTVNSDGTYRIGQLPPGDYTMAAGTGSPVNVNVSLGGTTTVN
                     LGSEGGVANLAAVEVVGSRVVNRVDVRSTESATNVTREELARLPVDQSVGSVALLAPG
                     VVASGATFGGLTFGGSSVAENAVYINGLNVTDPYRRQGFSSVPFAFYQEFQVKTGGYS
                     AEFGRTTGGVINAVTRSGGNEFHGGLEATFEPSAWASSKEDHFHNDGTLDERNRTSRD
                     ASPFYKANIWASGPIVKDRLFVFAMYEKRKSDPRDIDTTQAWFTKSDNDFWGTKFDWR
                     INDDHLVELLAFSDKADTLTSNYFYNWDTAVLGRKKGEGSTSSGGDNWSLTYTGHFTD
                     NFVAKAMYGVNKRSAVSGSPSDASCSIVTRSGSYTSKFGPETTPEGCHPTDSSIASRF
                     DKREAVRLDFEWTLGDHLLRFGLDQENMDSNSSVVYPGDGVSYQVQAATPGGALNGTV
                     VPAGATAIVDARRYITGAPVTNETQALYFEDNWSVTRDLLLNLGIRVDKFHNKLASGA
                     SFAKADFSDMVSPRLGFSWDMKGDGSTKVFGNAGRYYIPLTNKLTDYFGGGTTDEHTY
                     YVFNGWAQKVDPVTGGAYLEPILGAQIGPVNDDGNVPAPDDVRTAVARDLKQVFQDEF
                     ILGFQQAINTEWSYGINATYRRVTRAVEDVRINNLAGCAWYSGDFPIINPGETNTLWC
                     PDTNDWVTFDNSQEGYIASGSGAKTGYKKPRRTYKAVEFQLDRAWDDRWAFNASYLWS
                     KSEGNIEGPVNSDTGYADTNLVQFYDHPAVNERYGVLFNDYRHQIKLRGSFKLNEMWS
                     FGATLSTRSGGPITAFGVRWPNDNRGFGPGEFSGGGSGWLCVANCASLNRQLVYTERG
                     AFGRMPWVTNIGANVTWTLPVEGIDLKARFSVYNLLNSQTVVNVHSRYEAQPGTPLPH
                     FGQGTVWQSPRYMNLVVTYAF"
     gene            13899..15350
                     /locus_tag="PK399_00975"
     CDS             13899..15350
                     /locus_tag="PK399_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPW11731.1"
                     /translation="MTEHRRNGMALPTLRALAVALALVVAPASAFDRTLQTPLPVPPS
                     GVIGMQDAYFAPEFWIARTPAADQVLMTPAAIQARNARLLQVDDSMHDLSALPATLDR
                     ARVAGWIEDLASPPSKPLWDETAKPVPKATLDAIVGNRAMGAIPASQPTRFGMAVQRT
                     AMRAFPTELRVFNRQGETDIDRFQESALFPGDPVAIVHASRDAKWLFVVSERYAAWVA
                     ATAIAEGGRDAVLGYGARTPYRIVTGAKPHTVFTREEPRLSELQLDMGVRIPLANVAL
                     DKPVNGQHPYASWILDLPVRGSDGRLGFAPALLPRNADSAADYLPLTRANLIRQAFRF
                     LGERYGWGHSYNGRDCSGFVSEVYRSMGVELPRNTSDQSVSPAFDRIHFETDTPRATR
                     MAAVDALDVGDLIYIPGHVMMFAGRIDGMPWVIHDTNGGSFLGADGTLQPMQLNGVSL
                     TPLVPLRFGKDHDYIDRITNIVRVARDKMKIIQ"
     gene            15347..16453
                     /locus_tag="PK399_00980"
     CDS             15347..16453
                     /locus_tag="PK399_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPW11732.1"
                     /translation="MKISEIRFGMLRVPLKTAFKTALRTVEQVEDVVVMIHTDTGHVG
                     YGSAPATAVITGDTHGSIMHAIRHYIAPRLVGQEIANLNRITHAVQASMEKNTSAKAA
                     VEIAVHDLWGQLYGAPLYQLLGGGEPAITTDITISVDYIDKMVADSLAAVDRGFESLK
                     IKVGKDIGLDIERTKAIHAAVEGRALLRLDANQGWTAKQAVYALQTLEDAGVRLELVE
                     QPVKAHDLEGLRYVTERVHTPVMADESVFGPMEVIELIRMRAADIVNIKLMKTGGISN
                     AVKIADICGLYGVDCMIGCMLESSIGVAAAVHLAVAKSDVISKIDLDGPSLCRFDPVS
                     SGVLFNESEISVTDAPGLGIRAIEGLVLVDEPLA"
     gene            16464..17312
                     /locus_tag="PK399_00985"
     CDS             16464..17312
                     /locus_tag="PK399_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPW11733.1"
                     /translation="MSALVKIRSERDRMSAIERRIADFVLDNAQLLRDYSSQQLANAL
                     GISQSSVVKFCQKLGFKGYPDLKLSISEAVVRADNGNGGTPSAANGDTHAETIGADLW
                     LRKTEAEQATRLINPPDAVEAAAGAIAAAGKVFIIGLGDDDIHARTLALRLSLLGILT
                     VHNFDPAHMSTSLSATNPGDVLLVFSEQGMQPALYQLCRYAREQHARVVTVTRHSSNP
                     LRAHADVPLLVSAHDERPHIAPLLYHSALQHLLDRVFAVLCESDPKRRARLQANLERI
                     REKGSE"
     gene            17417..18187
                     /locus_tag="PK399_00990"
     CDS             17417..18187
                     /locus_tag="PK399_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11734.1"
                     /translation="MPIRRIAALLLTTVLAACAHAPQASTGTNTWSDAGQLVLVTTAD
                     WDATTGELRRFERDGTGWREVGEAAPISVGRTGTAWGIGLNAARSDGPVKREGDGKAP
                     AGVFAIGTAFGYAAGATTGLEYKGMGFNDWCIDVPESPLYNRIVDRSLAKAPGLDKSS
                     EPMRLDLHANGDQRYRQGFVIEHNANGTTPRGGSCIFAHLWGSPGQATAGCTAMAPET
                     MQPLLAWLDRNRKPVFVLLPKAQYAALKHDWNLPEIAR"
     gene            18184..19440
                     /locus_tag="PK399_00995"
     CDS             18184..19440
                     /locus_tag="PK399_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPW11735.1"
                     /translation="MSQTKRVLSGLAAGIVVGILLAWLDAPLALQVADIVEPFGKLWL
                     NALQMTVVPLVLALVIVGVNQASDAAQSGRVARRSIIIFVILLTAAGTMAALLAPLAL
                     SLVAPDPAVAAALGGSSVELPATTGGWASALTSIVPSNAVAAAAAGAMLPLVTFALFF
                     GFALTRIEPARRTQVVGFFQAVSDAMIVIVHWVLWVAPLGIFALVLALCARAGLGMLQ
                     ALGIYILLECALYALAVVLLVVIAVVFGKQNPGHFLSGITPVMAVAASTQSSLATLPA
                     MLDSAQTRLGFPARIAGLVLPMAVSLFRITSPIQYIATACFIAWALGIDLTFAHLATG
                     VALSVLVSMGSVGLPGQAIFLATNLPVSSAMGLPIAPLPVLMAVDAIPDVLATVGNVT
                     ADMTATALVASRDDGDGEPVPPATTA"
     gene            19635..22118
                     /gene="thrA"
                     /locus_tag="PK399_01000"
     CDS             19635..22118
                     /gene="thrA"
                     /locus_tag="PK399_01000"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009603901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HPW11736.1"
                     /translation="MVSSQVAQSPSCRAHKFGGSSLADASRIRHVASLMLGAGEPRQV
                     VVVSAMQGTTNALIAMTTAAAAGEDWQAAWSALRTQHADAASALLEAPAELHAWIDAR
                     FADIADLLRAVSLLRQPGRAALDRIQGMGEVLSATLLQAHLRARGADYALLDARDVLL
                     VRHAELGAVVDWDESARRLDAWRAANPQPRVVVTGFVAGGEDGLPTVLGRNGSDFSGA
                     IFAALFDAAELHIWSDVDGVLSADPRLVPEAVSLASMSYREACELAYFGAKVIHPQTM
                     TPAIARGLPILMRNTFKPEFPGTRIDADGDGGHGGPVKGLTLDPGLALVSLEGAGLIG
                     VPGTAERVFAALHAARISVVMISQGSSEHSICSVVRERDAEAARRALLDAFSRELDGG
                     QVQGVQVVPGISVLAAVGDGMAGKPGVAAQLFAALARARVNIRAIAQGASERNISVAI
                     AAEDATRGLRAAHAAFWLSPQTLALAVIGPGKVGAALLDQLQAALPRLRSEANVDLRL
                     RALATSRRMWLCDHGDPADWRTRIAEEPQPADLDAITTHLLDSHLPHAVVIDCSASDS
                     VAAQYPAWLAAGIHVITPNKHAGAGPLPRYAAIREARASSGARFRYEATVGAGLPVIS
                     TLRDLLDTGDELVAVDGILSGTLAWLFNKFDGSTPFSDLVREAHALGYTEPDPRDDLS
                     GMDVARKLVILGREAGMALSLEDVRVQGLVPEALRDTDRDSFMAGLASLDAPMQAALE
                     AARARGCVLRYVARLQRDGNASVALQELPADHAFAHLRLTDNCVQFTTRRYRDNPLVV
                     QGPGAGPEVTAAGVFSDLLRLAAALGARL"
     gene            22127..23044
                     /locus_tag="PK399_01005"
     CDS             22127..23044
                     /locus_tag="PK399_01005"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPW11737.1"
                     /translation="MEARAFSPGSVGNVGVGFDILGHSIAGIGDIATVRRIDEPVVRI
                     KAIRGAVTDLPLEAERNTAGGALISLREALALPFGFEIELDKGIPLGSGLGGSAASCV
                     AALVAANALLDAPLDAHALYPHAMAGEAIASGGRHGDNVGPMLLGGLVLATADRLTKI
                     AVPAAWHAVVVHPHAVLETKHARAALAGSYALKEFVAQSAQLAQVLLGCERGDAALVR
                     AGLDDVLVEPRRAPLIAGFAAVKQAALDAGAMGASISGAGPSVFAWCEDEATAQTAGA
                     SMAAAFASAGFDSDVHVGPVAGPAAGVLA"
     gene            23041..24333
                     /gene="thrC"
                     /locus_tag="PK399_01010"
     CDS             23041..24333
                     /gene="thrC"
                     /locus_tag="PK399_01010"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPW11738.1"
                     /translation="MKVLSTRGIASPVAISQAIAAGLAPDGGLYMPEHWPTFSPADFA
                     GDETLAAVGKRLLAPFFADDAIGERLDAICDDAFAVTPPLRRFGTGDDFVLELFHGPT
                     AAFKDYGAAFLAGCLGALPRGEVPLTIVVATSGDTGAAVGAAFHRKPGFRVAILYPDG
                     RVSPRQAHQLGCFGDNVRAFRVDGSFDDCQRLVKSALSDAEFRSEVPLGSANSISLGR
                     LLPQMTYYAHASLMHWRKHATTLNFVVPTGNLGNGLACVLARRMGLPIGEIVLASNAN
                     AVLPDFFAGGDYTPGPSIATLANAMDVGAPNNFERLQWLYPDAGGLRALLSADAVDDA
                     TIRATIVDAWQRQGEAICPHTACAVHVLQRRRAAGASGDWVAVSTAHPAKFPEVVEPL
                     IGQAIALPPALAAMLERPSHAQALADDGEALRDLLRHG"
     gene            24432..24508
                     /locus_tag="PK399_01015"
     tRNA            24432..24508
                     /locus_tag="PK399_01015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24466..24468,aa:Arg,seq:cct)
     gene            24570..26117
                     /locus_tag="PK399_01020"
     CDS             24570..26117
                     /locus_tag="PK399_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW11739.1"
                     /translation="MSHPVLTALGLQAVESGTTISGTYVGHGEWSKTSDAGVIEPVNP
                     TTGEVLGRVHASSQADYDLIVERAQAAFKVWRTTPAPRRGEAIRLCADALRVHKDALG
                     SLVALEMGKSKPEGDGEVQEMIDIGEFSVGLSRQLYGLTMHSERPGHRMYEQWHPIGI
                     VGIISAFNFPVAVWAWNAFVAAICGDISIWKPSPKTPLSAIASMKICNEALAKAGFPD
                     IFFLFNDAGSDLAQSFVDDKRVPLISFTGSTKVGRMVGERVARRMGRSLLELGGNNAI
                     IVDETADLKLAIPAIAFGAVGTAGQRCTTTRRLIVHESIYDDVLAKLVAAYKQVEKKI
                     GDPTDPANLMGPLNSKDGVDAYLDSIAKAKAAGGTVETGGEAIDRPGNFVLPAIVTGL
                     TNEAEIVQHETFAPILYVMKYKDLDEAIEMQNAVPQGLSSSIFTTNLKRAEAFLAASG
                     SDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINYSDSLPLA
                     QGIKFDL"
     gene            26128..26415
                     /locus_tag="PK399_01025"
     CDS             26128..26415
                     /locus_tag="PK399_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010378557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HPW11740.1"
                     /translation="MNAMPRQTSTLAIISLVAGILGWTVVPFFGSIAAVVCGHMARKE
                     IRLAPDRLEGDGLAIGGLVLGYLSIAMAVLVVVAIFLFFGGLAAFLAFANH"
     gene            26420..27475
                     /locus_tag="PK399_01030"
     CDS             26420..27475
                     /locus_tag="PK399_01030"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HPW11741.1"
                     /translation="MYALSRPFLFAFDAERAHGLGLASLEAAYRSGLNPLIAKPPKPL
                     PTKAFGLTFPNPVGLAAGLDKNGAHIDALLALGFGFVEVGTVTPRPQPGNPKPRMFRL
                     PRQEAVINRLGFNNEGVDALVRNVERARRIGGLLGINIGKNKDTPNESAEDDYLHCLR
                     KVYALADYVTVNISSPNTAGLRELQEEQALRRLVSVLRDEQEKLAGQHGKRVPMLVKI
                     APDLSESDIDAAGRVLGDLAVDGVIATNTTIARDGVEGARFADEVGGLSGAPLMGKST
                     AVLRMLRTRLPESIPLVGVGGILHGADAATKQAAGALLVQVYTGLVYRGPALVGECVD
                     ALRRRKEAPSSTPLPLA"
     gene            27472..28515
                     /gene="murB"
                     /locus_tag="PK399_01035"
     CDS             27472..28515
                     /gene="murB"
                     /locus_tag="PK399_01035"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPW11742.1"
                     /translation="MTAGYRIVENARLDARNTFGVAARAPMLVDVADAAALPELFGYA
                     MLRDGPVLVLGGGSNLLFAGDAPGAVLALSSQRIELLREVGDAVIVRADAGVGWHDFV
                     LWTLGHGLCGLENLALIPGTVGAAPIQNIGAYGVEVRDAIRAVHAFDRRDRGFVRLDA
                     GDCDFAYRDSLFKHDPDRYIVTAVEFVLSRTPDLRLEYAGIREELTAMGIDGTPRASQ
                     VAEAVIRIRQRKLPDPARLGNAGSFFKNPIVPMAIAEALQATHPAMPVFRGAGDDTRK
                     LSAAWLIDQCGWKGHRHGDAGVSAEHALVLVNHGQATGLQLLDLARRIAASVRDRFGI
                     AIEPEPRIIGAAW"
     gene            28536..29453
                     /locus_tag="PK399_01040"
     CDS             28536..29453
                     /locus_tag="PK399_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW11743.1"
                     /translation="MRAALLMLASTVAFGLMALCIRLASQTEPTWEVAFFRNAFGFAA
                     LLPMLAWPLLRSPRPLAQFAANVRTDQLPRYFVRCAIGIVSMYCGFWAIGHLPLAQAI
                     SLAYSSPIFITIAAALWLGEHVRMRRWLAVAAGFVGVLVIVRPWSHAFTTGSLVAVAA
                     AMMNALVAIQIKQLSRVDKADTIVFWTYILWVPMSLIPALFVWHPPQGITWLWLVLCG
                     VLGTFGQLLWTRALKLGEVSALTPISFVQLPIVTLAGWLLFAERVDAMTILGAAIILS
                     ATFYIAHREAVLARKAASQAPSEAAVPGE"
     gene            complement(29458..32196)
                     /locus_tag="PK399_01045"
     CDS             complement(29458..32196)
                     /locus_tag="PK399_01045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPW11744.1"
                     /translation="MAQGPHNDPERTELLATRSPVAGATTGEAAALPSGARLGRYRID
                     GLLGRGGMGEVYRAEQLEPVRRTVAIKLLRAQKLDARHLAYFEVERQLLAQMRHPAIA
                     QVFDADTTADGHPFFAMEFIAGSPITAYCDAHAMPTDARIALFIRVCEGVQHAHQKGV
                     IHRDLKPGNLLVDEVDGRALPKIIDFGIATATSLTEGREVAGTPDYMSPEQAGGDQSR
                     VDTRSDVYALGVVLSELLTGRRPLAAGETVTDHDRTLRLPSAQLSTLSPADAERLAKA
                     HGEPVSRMRRVLRSELDWVVAKAMRHDRNERYASAAALADDLQRFLDGRPLQAVPPSR
                     GYALRKFAQRHRTGIAAAALVALALVGGLALSVYGLLQARAQRAIAEQRSVELEKVAA
                     FQQSMLQGIDIEAMGIGMADGLRKQLASADVADLAALERALGQASTADIARGLIDRDI
                     LVNAERAIDRDFAREPALAADLRESVARVRVALGLPRDAARGFAQVADYRAGALGRAA
                     PETLRARQAQVSAMLEAGEAKPALALVQASLVDAAALLPSDALRIKLRLNEAEAVASL
                     GDRPKGRALLEAVRADSLELRGERDPTTMEVTNSLAILTGRMGEAKEGRALMERLVPL
                     RTRVLGPGHADTLASLHNLAVMRIMTGDKDGAVALQQGLVQTQVRRLGAEHPQTLGER
                     GNLANMLNDSGKPAEALPVAEAVVEARTRVLGAEDAQTLRSKLNLSTIYARLKRFDES
                     IALQQQVADARTRRLGPDHPDTLFIQLNRAATLYQAGQPKAALAQLDRWLPVARKQLG
                     DKHPQVQMGFDIRAQAADELGDKALAMASWRTLLEIREGALGADDARTVDAAWQLEGM
                     LRSAGNRKEADALRARYIAPLLEAKEDSLSEEQAAKRKDIIETEREEARAARTAAK"
     gene            32289..33188
                     /locus_tag="PK399_01050"
     CDS             32289..33188
                     /locus_tag="PK399_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW11745.1"
                     /translation="MEPDTELQDTTQRTVLSHGPLRSAPRSACVVVIHGEGLGRRADI
                     TDTAVLVGRSQEADLVIAHKSVSREHCRVWREGNGYRIRDLGATNATRVNDVRIAGET
                     TLADGDQVTVGESILKFISQDSVEASYHEEIYQLATHDALTELYNRRHFIEMADKEIA
                     RSIRHARPLSLCIIDVDLFKPVNDRYGHISGDEVLRQIALLLRRHVRSDDIAARIGGE
                     EFALLLPECDAEAARVFAERLREAVAAAPFAPGGEPQRITVSIGIAELAAERDTRPAL
                     MAAADTALYRAKSEGRNRVCVEA"
     gene            complement(33192..33920)
                     /locus_tag="PK399_01055"
     CDS             complement(33192..33920)
                     /locus_tag="PK399_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPW11746.1"
                     /translation="MTLPHDPRLPHSAREAMDVARDDARFGIAFIAQRWRRLLLAFAG
                     LLLPLWAFGELADEVREAEVFPFDAPILQFAQSVAHDGFDSTFLVFSRLGYEWGVVPL
                     DVLVVLVLAAMRKLREGLFAGVAIIGSALLNLGAKQLFARQRPALWDSIAPESTFSFP
                     SGHAMGSITIAMVLVLLAWHTRWRWWVTLPLAAFTLMVGLSRVYLGVHYPSDILAGWA
                     VAIAWTVGAYLLVFPHGQRPWQER"
     gene            complement(33999..35963)
                     /locus_tag="PK399_01060"
     CDS             complement(33999..35963)
                     /locus_tag="PK399_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPW11747.1"
                     /translation="MDGKRTSVPLYLHITLLSFGLVLATGLVLRWFGDAIPGVAEFGL
                     LLGAVAVVALLASRRIAAPLAALAKEAEAVRHFDFSDHPVIHSPVQEIDQLGGAFDQM
                     RDAVRRFLRINRRLATEDDLEALQPWLLDKMVDIAGARAGVLYLVDAADDALRATAMD
                     TSGGRATAVGGLPVLAQDALPRLLADARATMRPVGGHLSGDDLRTLGLEPEFAAFGTL
                     ALPLQDRREQLLGMLLLFKDGEIDEAAASFIAALAASAATSLETQELLRGQKALMEAS
                     IRMVAGAIDAQSPYTGGHCERVPELTFMLARAACAAQDGPYAGFDLDAEQWEALHIAA
                     WLHDCGKVTTPEYVVDKATKLETVNDRIHEIRTRFEVLKRDADVDYWRGVAEGGDEAS
                     LRQARDTLQATLDDEFAFVAQCNVGGEFLSPDKQARLREIGARRWQRTLDDRIGLSRD
                     ELARKPPQAMPLPAEETLLADKPEHRIARPESERIPADNRWGFRMDVPELLYNRGELH
                     NLSISRGTLTAEERYKINEHMVQTIVMLGELPFPRHLRAVPEIAGGHHEKMDGTGYPK
                     RLARDELSPLARMMAIADIFEALTAADRPYKPGKTLSEALSIMAKMRQDQHIDPELFE
                     VFLRSGVHHDYAQRFMRAEQVDAVRIEDYL"
     gene            complement(36031..>36256)
                     /locus_tag="PK399_01065"
     CDS             complement(36031..>36256)
                     /locus_tag="PK399_01065"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPW11748.1"
                     /translation="NMTLAVMGCIVNGPGESRHANIGISLPGTGETPAAPVFVDGEKT
                     VTLRGDNIAHEFVALIDRYVDTRYRAGRPG"
BASE COUNT         5581 a        12617 c        12269 g         5789 t
ORIGIN      
        1 atctccagca ccttccccac cgccgcaaac gcgaacgcac cggtgacatg ggtggccgcg
       61 cccaggccgg caccgcaatc cagcttcaat gcctcgtcct tgcccagttg cgggcggatg
      121 ccgcagacgc tgccgtcggc ctgcggatag cggacgttct ccagcgagta gatcgccggc
      181 accccgaaat agcggtcgtg gttcttgggg aagttgaatt cgccgcgcaa cttcttgcgg
      241 atcagcgaga gcatcgcatc gtgctcggtg cgggagaggt cgcgcacgcg gatctgggtc
      301 acgtcgctgc gtccgccggc ggagcccacc gtcacgatcg gctgtttgcg gcgacggcag
      361 tacgcgatcg cctcgacctt gctgcggaag ctgtcgcagg catccagcac cagctcgccg
      421 ggcgcatcca gcatgtcgcc caggttcgtc ggcgtcagga actgcggcct caggtcgagt
      481 tcgatgccgg ggttgatcgc acgcaggcgc tcgcccatcg cctcgacctt ggcacggccg
      541 tactggcctt ccagggccgg caactggcgg ttggtattgg agacgcagag atcgtcggca
      601 tcgatgaggg tgagcttgcc gatgccgctg cgcgccagcg cctccaccgc ccaggagccc
      661 accccaccga tgccgatgac ggtgacatgg gaggcgttga agcgcgccag cgcgccgctg
      721 ccatacaggc gatcgatgcc gccgaagcgt tcgcgcaatg cattgtccat gcgatccagt
      781 ttaacgttcg ggtcacggcc gagcgcatcg ctgccggacg ccttgccccc tgagtcaggg
      841 ggcgattcgc gccgcaggtg cgaatggggg tatggaagaa catagcgggg cccgaagttt
      901 gcgcagcaaa ctttgggaac tcctgggcgc agcccgggag ttgcaagcct tgttgcggtg
      961 cgccatgctg cagccggcgc ctcttagagt gtttgctcca tgaccgcgac tcgcatcatc
     1021 aagaagtatc cgaaccgccg cctctacgac accgagatct ccagttacat cacgatcgag
     1081 gacgtgcgcc aattgatcat cgatggcgag accttcgagg tgcgcgatgc caagaccggc
     1141 gaggacctga cccggcaggt cctgctgcag atcatcgccg agcacgaaca ggacgggcag
     1201 ccgatgctgt ccacgcagtt gctgagccac ctgatccgct tctacggcga ttcgctgcaa
     1261 gggttcatgg gcaactatct ggagcgttcg atgcaggtct tcctggacca gcagcaacag
     1321 ttccgccagc agatgggcgg actgatgggg cagacgccgt ggaccatgct caaccagttg
     1381 accgagcgca acatggacgt ctggaaggac ttccagaaga acctggtcgg cggcatgggc
     1441 agccccaccg gcagcgccca gcgggatcgc gacccgcgca agccacgctg aggcggggcg
     1501 tcatcgcatt gcctgcgtga aaacgggcct tgcggcccgt tcttgtttcg acctgcggaa
     1561 gcgcggctgc gcgatcaggt ccggccggtc gtgccgcagt acttgtcgcg gaaggcgagc
     1621 gccttcggca tcagcgcctg cagttgctgg atgcgggtct ccggactggg atgggtggac
     1681 gcgaattccg gctggccctg gccgccgctg gcctgcgcca tgcgctccca cagcggcacg
     1741 gcctcttgcg ggtcgaagca agccgccgcg gccagcatca agcccacttc gtccgcctgc
     1801 gtctcatgtc cacgtgcgta cggcaaggcc gaggtcacgc cgtaggccga catcaccgtc
     1861 tgcatggtgc cggcgtccat cccgccggcg gcgccggcca tctgcccgat ctgctccagc
     1921 ttgccgcgcg ccatccgctg cgcaccgtgg cgcatcagcg catgggcgat ctcgtggccc
     1981 atcaccaccg ccatgccgtc ctggttcttc gccaccggca ccaggcccgt gtacaccgcc
     2041 atcttgccgc ccggcaacgc gaaggcgttg gcctggtcgg actggatcac gttgacggtc
     2101 cagtcgaaac cggcggcgaa gttgcgtgcc tcgatgccgc gctcggccgc gaggtcgcgt
     2161 tcgacatcgt ccaccttgtc gatcaggcgc gcggcgatgc cgctgatctg acgggcaatg
     2221 tccgaattcg gatcgaccgg gcgctcctgg ctgaggatct cgcggtacgc ctgcaacccc
     2281 atggcgcttt cctgctccgc atccagggtc ttgtcgatca gcacctgctc gccggtgtac
     2341 ggatccaccg tgcggttgga gaagtagtag tacgcgccat acaagagcat cgcgccgacc
     2401 accaacagcc gcaggccgcc accgccgagg cctcggcgcg gttgccggtt gtaaccgttg
     2461 cccatcggat cctggttcat cgccccactc cctgcgttgg aatcgccgcg ccgggcgagt
     2521 acccggcaac catcaaatct gcctgaccaa tcggaacccg acccgcgcat tggtcgcatc
     2581 ggtgccgccg gatgcgcgcc atgccgaacg cacctgcgcc ggcgcgctgg cccaggaacc
     2641 gccgcgatac atgcggttgc ggcaaccggg gttgatccac gccgccccgg tggccggtgc
     2701 gcgtcgatac ccctgatgcc agcagtcggc cacccactcg gaaacattgc ccgccatgtc
     2761 gtgaagacgg aatgcattcg cggcgaagct gcccaccggc gcaggccccc accagccatc
     2821 gccgtagccg acgaaggcat tgctccagcg cctgccctgt ggcgaaacat cgcgcccgcc
     2881 ggtcaggttc tccacgccgt cgggtggcga accgttgccc cacggatagc gcgtggtgct
     2941 gcccgcgcgc agcgcgtact cgaattcggc ctcgctgggg agccggtaat gcgcgccggc
     3001 ctgctccgac agccacgcgg cgtaggcctc ggcatcgcgc ggggtgacat ggatcaccgg
     3061 catgtccgca gccgccggcc ggccgtcgta gccggagcgc cagtcgatac cgctgccgcg
     3121 cacgaagttt ccgctgcgca cgtcgtaggc gagcgaatgg ccgcgctcgg tggcgcgcgc
     3181 ctcgtagccg gtggcggcga cgaagcggcc gaactcgccg acggtgatct catggcgggt
     3241 catcgcgaag ccgcgttcga gccgcaccag gtggcgcggt cgctcgtcct tgctggcatc
     3301 ggcctcgtcg tcgggcgcgc ccatgtggaa cgccccgtgc ggcaccacca ccaggccggg
     3361 tccgcggctg ccgtcatcga gcccgtcggt gaacacctgc cccggccgga acagcccgta
     3421 atgcccgacc aggtcgatgc gctggcgcaa ttccatgctg gcgggatcac cggcccgggc
     3481 aatcagcaac atgtccgcaa ggcgttcctg cgccacccgc aggccatccg gttcgccgag
     3541 tgcgatcagc ccttcgtcgc gcaaccgccg gatgcgctcg gcgcgcatca cttcgatgcg
     3601 tgccgccgcg gcagggatgg tctgcgggtg ctggcgcacg cgctgggcac gggccagatc
     3661 cgccctggcg gtgtcgaaat cgccctgctt cgcggccgct tccgcgctgc ggatcagggc
     3721 ggactccacc gcaccgatgc caaggtgcgc gcgcatctgc ccgggtcgca gccgcagtgc
     3781 atcacggaac gcgtccagcg cgccgcccgg cgtatccagt gctccggcca ggagtgcgcg
     3841 ttcgccggcc aggctgcgct cgcgcgcctg ttcggcggca tcgaccgcct gcaggtacgc
     3901 cttggcacgc accgcgtccg gccacagggt gcgcaggacg gtggcatggc gctgggcgaa
     3961 gcgcagcgcg gccaggtctt cgtcggcggc atccagggct gcgttggcct gtccgatcag
     4021 tgcggcttgc gcgcgctcca gcaacttcgc atcgccctct tcgcccggat actggcgctg
     4081 caatgcgatc aggatcggga tcgccgatgc gctggtctcg aacaggtcgc cctgctcaag
     4141 cgcacgctgg gcggccttgc gcgcggccgc cggatcatcg acctgcaccg ccggcaggct
     4201 ccagctgagc acgccggcca gcgcatcgtc gccactgatg ctgacgctgc cggcaggcag
     4261 ttccggctgc gcggcctgcg gggtcggcgc cacgggcgtc tccggcccgc aggcggccaa
     4321 cgccagcggg acgaacaggg caatgacggt ccagcctgcg cgcaaacgac actccgggac
     4381 gccggcaaca ggcccgcgac atggttcggg cgaacttagg ccaagtcgtc gacaccggca
     4441 accgcataat ggcggcatga gccaatggat cgacaccccc gacgcgctgc gcgcgcgcct
     4501 cgccgcccca cccgccagca tcgggctgga caccgaattc atccgcgaac gcacgtactg
     4561 gccgcaactg gcgctggtgc agatcgcgct gggcgatggc ggcgacgtgc tgctggtcga
     4621 cccgctggtg cccgggatgg ccgaggccct ggtgcccctg ctggtggatc ccggcgtgct
     4681 caagctgatg cacagcccca gcgaggacct ggtcgccttc cagcacacct gcggcgtgtt
     4741 gcccgcgccg ctgttcgaca cccaggccgc ggccgcgctc tgcgggatcg gcggcggcat
     4801 gagttaccag aagctggtac tcgcggtgac cggtgtcgcg ctggagaagg gcgagacacg
     4861 ctccgactgg ctgcgccgac cgctgtcggc atcgcaactc gagtacgccg ccgatgacgt
     4921 gcgccacctg catgccatgc atgcgtcact gcagcagcaa ttgaacacgt tggggcgcag
     4981 cagttggctg gccgaggaca gcgcccgcca gctcgcgaat gccgccaatg aggagggcga
     5041 ccgctggccg cacctggtga tgcgcagcgc gcagttcctg gacgcacccg cgcagcgccg
     5101 gttgctgcgc ctgctgcgct ggcgcgaagc gcacgcgcgc cgcagcgacc gcccacgcag
     5161 ctggatcctg gacaacgagc tggcggtgac cctggcccgc gccaaccccg cagacccggc
     5221 cgccctgcaa gcgcagctgg atgccacgcc gaaggcaccg aagagcctgc gcgatccgat
     5281 ctggcaagcg ctcaccaccc ccttggcgga cgaagacgaa gccccgctgg cgcgtggcga
     5341 agaccgcgac aagaagcaac tgcgcaccat gcaggacgcg gtgctggccg tcgccggcga
     5401 actcggcgtg ccggaaggcg tgctggcctc gcgcaagctg ctggaggcgc tgcaggacgg
     5461 cggcggttgg agcggcccgc tggcgggctg gcgacgcggc gtgctggaag cccgactgca
     5521 accgctactt gcgggcacga ccacggcgtg atgccgattc gcgcggtgta ggatcgaccc
     5581 gaatcctggg gaggacaccg tgatgcgtca ttcgctgctg gtcgccgcgc tgctcgcgct
     5641 cttgccttgc tccgcgctcg ccgccgatgc gcagatggaa gcgctggcca ccaaggtgga
     5701 cggcaaggtc caggcgtggc ggcgcgacat ccaccagcat ccggaactcg gcaaccgcga
     5761 agtccgcacg gcgaaactgg tcgcggagca tctgcgcgca ctgggattcg acgacgtgcg
     5821 caccggcatc gccaccaccg gcgtcaccgc ggtgctgcgt ggcggccaac ccggcccgcg
     5881 catcgcgctt cgcgccgaca tggacgcgct gccggtgacc gaacgcagcg acctgccgtt
     5941 cgcttccaag gcgacctcga ccttccgcgg tgaacccgtg ggcgtgatgc atgcctgcgg
     6001 ccacgatgcc cacgtcggca tcctcctggg cgtggccgag gcgctggcct cgatcaaggc
     6061 ggagctgccc ggcgagatcc tgttcgtgtt ccagcctgcc gaggaaggcc cgcccgacgg
     6121 cgaggaaggc ggtgccgagg aaatgctggc gcaggggatc ttcgaccgtt tcaagccaca
     6181 ggcggtcttc gggctgcatg tgttcagcac gctcaatgcc ggccaggtgg gctggcgcag
     6241 cgggccggcg atggcggcat cggaccgctt caacatcgtc gtccagggcc ggcagaccca
     6301 cggctcgcgg ccgtggggcg gcgtggaccc gatcgttgcg tcggccgacc tcatcggcac
     6361 cgcgcagacc atcgtcagcc gccgccagga catcagcagg ctgccggtcg tgctcagctt
     6421 cggcgcgatc aagggcggca tccgctacaa catcatcccc gacgcggtgg aactggtcgg
     6481 caccatccgc accttcgacg agggcatgcg ccaggccgtg ttcgcggacc tgaagaacgt
     6541 ggccgaaagc gtggcgaagg cgcatggcgc gaccgcggtg gtgaaggtgc cggacaccaa
     6601 gggcaacccg gtcaccgcca acgatcccgc cctcacggcg cgcgtggtac ccagcctgcg
     6661 caaggcggtg ggcgacgcca acgtggtcga gatgggcctg gtcatgggcg ccgaggactt
     6721 cagtttctac gcgcgcgaag tccccgggtt cttcttcttc gtcggcgcca cgcccagggg
     6781 ccaggacgcg atcaaggcac cgtcgaacca ctcgccggaa ttcttcctgg acgaagaagc
     6841 gctgaaggtc ggcacccgcg cgatgctgca ggtggtgctg gaccaccagc acggcactgg
     6901 cggctgaatc cgggaccgcc cgcatccggc ggcgcgtgcc tctggcgaga ccggcggcgg
     6961 ctccgtataa tcggtcgcgt tgtacatcgt ggggcggtag ctcagctggg agagcgccac
     7021 gttcgcatcg tggaggtcag gggttcgatc cccctccgct ccaccacgat tccaaggccg
     7081 gccccgtgcc ggccttgttg tttctggcga caagccatca atcgcgcagg tctagtcgcg
     7141 caagaacagc gcaccgatgt cgagcgcggt ccggtacggc gcggggtcgt tgcggttgct
     7201 cagcaggatc acggtcagcc gctgcgccgg ccagcgcacg atcacgttgc ggaagccgat
     7261 ggtctcgccg gaatgccaga gggtgtcgcc ggtgatccgc cagccgaagc cgtaatgcgt
     7321 ggcggcgctt tcacccgtga cctcgacctg cttgccgaag gccagcgcgc gcgaggcgtc
     7381 attcaacagg cgatcgtcgt acagcgccgc atcccaacgc gcgaggtcgt gggtgttcga
     7441 gtagatcccg ccatcgccca gcaccgcgct ggtcgcgctc tggtcggtgc gttgccactg
     7501 cccgtccaca tcgctgtagc cccaggcgcg gttcgacacg tcagcgctac ccgccacgta
     7561 ggccacggtg tcgtgcatgc ccagcggcag gaagatgtgg ctgcgcagga acgccgggaa
     7621 atccatcccc gacgcgcgtt ccacgaccag cgccagcagc gcatagccgc tgttgctgta
     7681 gcgatacgcg ctccccgccg ggaaggccaa tcggttttca cgctcaagca gcgcgagcac
     7741 gccggcatcg cggatctggc cctcataggg ttcggccatc aggtcttcgt aatcgagcag
     7801 ccccgaggtg tgggtgagca ggtggcgcag cgtgatcccg gcggtcgcgg gcggcagcga
     7861 gggtagccag cgcttcaccg ggtcgtcgat cccgaggctg ccgtcctgcg cgagcagcag
     7921 gatcgccgca gcggtgaact gcttactcac cgaggccagc cggtaattgg tcgccgcgcc
     7981 ggcttcgatg ccgtgctcca ggtcggaacg cccgtagccg cggtgcaccc gcgcctcacc
     8041 atcgcgcagg accagcaggg acgcaccggg gacgtcgccc tcgtaacgtt gcatcaggcg
     8101 ttcgatcgcg gcatcgggca tcggggactc gggggcagtc acgggcacgt cgtagtacgt
     8161 gccgggggag ggttcgggct ggaaggtgta gtgccaccat tccatcggat agttacggaa
     8221 tccggcggcc tgcatggcct cgcgcagtcg caggcggttt gctcgctggg cggcatcgac
     8281 atcgggtgag tcggtgttgg cgcgggtacc gaagaaatcg aacgccgtgc ccatgtccag
     8341 cggcgtgcag cccgcgccgg acgcgtcgca ttgcaacagg gtcaggtcca cggtggcgcc
     8401 acggctgtgc ccggacatcg gcgaaatgta ttcgccaacg agttgcgcct tgcccaggtc
     8461 cgggtaatgc gtggccttgg tgcgctgatc cgcgccatcc tcgacccagc gcatgaaatg
     8521 cgcgaccgcg cgcgccggcc ggtagcaatc gaagatccgc aggcgctggt gccgttcgcg
     8581 caatgcggcc tcgaccttcg ccagcgcgtc ggcggcttgg cgcttcaggt agcaacgcgg
     8641 cgcttcgtag ccgtccaccc gggtgccgac gaaattgtcg ctgccgaaat agcggatgtc
     8701 ctgggcgatg ccgggcacga cggcgcggat gtcgaccatg cccgcctctt ccatcgtggt
     8761 cgcggatgac agcgtcggcg gatcggccgc catcgtcgcg ccgcatgcag ccagcaacaa
     8821 catcgcccat ccgaggcgaa tgtcagtcac agccgcgcac ccgcacgaaa tcgaggtctt
     8881 cgtagtcgga actgaaatcg ccggcgggat cgatcttggc catgcgcatg cgcggcccgc
     8941 tgctgcccac ggagaacgcc aggaacgcat ccaggtccac cgcgggatcg tcccagtgca
     9001 acaggtagcg accatcgagc tcaccgacgc gcccgtgcat cttcggcgac ttcagcgagc
     9061 gccattccac cgcgccatcg accgcgcaca ggcgtacctc gccgaaccag gggtcgcgcc
     9121 acgtgcccag ccaacccgtg agttgcccgg gtgcgaccgg gcttcgcttg ctcgcatccg
     9181 gcaacggcgg cttcggcgcg gcggcgccat tgggcgctgc cggcctggcg acttcgtcgg
     9241 catatcccgc cacgcccagc gatttctccg gcgcggtgaa cagcttcagc agcacctgcc
     9301 ccagcacggt gcgcgcgttg ctgccgtcgc cgttgatcat gaacacgaag ccggacctgc
     9361 gatccggcaa cagcatcatc atcgagtaca tgccgcccag cgtgccggtg tgcgacaccg
     9421 tccattcgcc gtccacgtcg gccaggcgga atccataggc ataggcgtag tagtgggtgt
     9481 tgtcccagcg ccggcgctgc gcggagatcg gcatcggcgt gcgcgccttc caaagttccg
     9541 cgcgttgcgc atcgccgagc cactgcttct gcgcgtccgt gggcaccagc cagttgctgg
     9601 cccaggccag catgtcgtcg aggtcacagc ggatgccgcc cgcgggcgcg gaggcgatcg
     9661 caggcacgat cgcgccatcg cgacgcatgg cttcgttgcg tgttccgttg cgcgtatgcg
     9721 gctgggccac gctggctgcg tcggccaggg cgaattcgcc gacgcggcat ccggacaggc
     9781 ccagcggctg gaacagttcg cgccgcacca ggtcctcgta cgacgcgcca ccggccgccg
     9841 cggccacttc accggccacg acatacagca ggttgtcgta ggcatacccg gcgcggaagc
     9901 cgtaagccgg cttgatgtgg cgcaggccgc ggacgatgtc gtcgcgactg aacaggttgg
     9961 gctccggcca cagcatcagg tcgccgccgc cctccggcag gccactgttg tgcaccagca
    10021 ggtcgctcac ggtcatgtgc cgggtcaccc acggatcgtg catcgcgaac tgcggcaggt
    10081 gcttcaccac cgggtcgtcc cagcgcagct tgccctgctg caccagccgt gccagcaccg
    10141 cagcggtcat cgccttgctg ttggaggcga tcttgaacag cgtcttcgtg gtcaccggat
    10201 cgccgctgcc ggccacggtt tcgccaaagc cgcgcgcata gacgaccttg ccgtcttcga
    10261 tgacgcccac cgcgatgccc ggcagttggt agcgatcgat ggtggcacgc accgcggcgt
    10321 cgatggcttg cggcgtggga ccttcgatgg gcgcagccgc gtgtgccgtc ggcatcaatg
    10381 agcatgcgag caccgcgaca cgcagcactc gtgcgcgaag gcgcggcatt tcccacgaac
    10441 gtcggaaggc tttgtccatc tgcgttccca tgcgctggat cagggagatc atcgcaccgt
    10501 ctccgccttc gcgcgtcgcg gtagtggcgg catgcgtcat gcaaaaaaga ggcggcccgt
    10561 gcggggccgc cggagaatta gtcccggagg gaggactagg ggttcggtgg gctgccgcgg
    10621 tcagaaggcg taggtcacga ccaggttcat gtagcgcggc gactgccaga cagtgccctg
    10681 cccgaagtgc ggaagcggcg ttcccggttg tgcctcgtag cgggaatgca cgttcaccac
    10741 ggtctggctg ttcagcaggt tgtagacgga gaagcgcgcc ttcaggtcga tgccttccac
    10801 cggcagcgtc caggtcacgt tggcgccgat gttggtgacc caaggcatgc ggccgaacgc
    10861 accacgctcg gtgtaaacca gctggcggtt caggctggcg cagttcgcca cgcacaacca
    10921 gccggatccg ccgccgctga actcgccggg gccgaagccg cggttgtcgt tgggccagcg
    10981 cacgccgaat gcggtgatcg gaccaccgga acgggtggac agcgtggcgc cgaagctcca
    11041 catttcgttg agcttgaagc tgccgcgcag cttgatctgg tggcggtagt cgttgaagag
    11101 cacgccatag cgttcgttga ccgcggggtg gtcgtagaac tgcaccaggt tggtgtcggc
    11161 gtagcccgtg tcggagttca ccgggccttc gatgttgccc tcggacttcg accacaggta
    11221 gctggcgttg aacgcccagc ggtcgtccca cgcgcgatca agctggaact cgaccgcctt
    11281 gtaggtgcgc cggggcttct tgtagcccgt cttggcgccg ctgccggagg cgatatagcc
    11341 ctcctgggag ttgtcgaacg tcacccagtc gttggtgtcc gggcaccaca gcgtattggt
    11401 ctcgccggga ttgatgatcg ggaagtcgcc ggaataccag gcgcaacccg ccaggttgtt
    11461 gatgcgcacg tcctccaccg cgcgggtcac cctgcggtac gtggcgttga tgccgtagga
    11521 ccactcggtg ttgatcgcct gctggaagcc caggatgaac tcgtcctgga acacctgctt
    11581 gaggtcgcgc gccaccgcgg tgcgcacgtc gtcgggggcc ggcacgttgc cgtcgtcgtt
    11641 caccgggccg atctgcgcgc ccaggatcgg ctccaggtag gcgccaccgg tgaccgggtc
    11701 gaccttctgc gcccagccgt tgaacacgta gtaagtgtgt tcgtcggtgg tgccgccgcc
    11761 gaagtagtcg gtgagcttgt tggtcagcgg gatgtagtag cggcccgcgt tgccgaacac
    11821 cttggtgctg ccatcgccct tcatgtccca gctgaagccg aggcgcggcg agaccatgtc
    11881 gctgaagtcg gccttcgcga aggatgcacc cgaggcaagc ttgttgtgga acttgtcgac
    11941 gcggatgccg aggttcagca gcaggtcccg ggtgacgctc cagttgtcct cgaagtagag
    12001 cgcctgggtt tcattggtca ccggggcacc ggtgatgtag cggcgcgcat cgacgatcgc
    12061 ggtcgcgccc gcgggcacca cggtgccgtt cagggcgcca cccggggtgg cggcctgcac
    12121 ctggtagctg acgccgtcgc ccgggtacac cactgagctg ttggagtcca tgttctcctg
    12181 gtccaggccg aagcgcagca ggtggtcgcc gagcgtccac tcgaaatcca ggcgcaccgc
    12241 ctctcgcttg tcgaagcggc tggcgatgct ggaatccgtc ggatggcagc cttccggcgt
    12301 ggtttccggg ccgaacttgc tggtgtagct gccggagcgg gtgacgatgc tgcacgacgc
    12361 atccgacggg ctgccgctca ccgcgctgcg cttgttgacg ccgtacatcg ccttggcgac
    12421 gaagttgtcg gtgaaatgcc cggtgtaggt gagcgaccag ttgtcgccgc ccgagctcgt
    12481 cgagccttcg cccttcttgc gccccagcac ggccgtgtcc cagttgtaga agtaattgct
    12541 ggtgagggtg tcggccttgt cggagaaggc cagcaattcg accaggtggt cgtcgttgat
    12601 gcgccagtcg aacttggtgc cccagaaatc gttgtcgctc ttggtgaacc aggcctgggt
    12661 cgtgtcgatg tcgcgcggat cgctcttgcg cttctcgtac atggcgaaca cgaagagcct
    12721 gtccttcacg atcgggcccg atgcccagat gttggccttg tagaaagggc ttgcatcacg
    12781 gctggtgcgg ttcctctcat cgagggtgcc gtcgttgtgg aaatggtctt ccttcgagga
    12841 cgcccacgcg gacggctcga acgtcgcttc caggccgccg tggaactcgt tgccgccgga
    12901 acgcgtcacc gcattgatca cgccaccggt ggtgcggccg aattccgccg aatagccgcc
    12961 ggtcttcacc tggaattcct ggtagaacgc gaacggtacc gacgagaagc cctgccgacg
    13021 atagggatcg gtcacgttca ggccgttgat gtagaccgcg ttctccgcca ccgaggagcc
    13081 gccgaaggtg agtccgccga acgtggcgcc cgaggcaacc acgcccgggg ccagcaaggc
    13141 gacggatccc acgctctggt ccaccggcaa gcgcgcaagc tcctcgcgcg tgacgttggt
    13201 ggccgactcg gtcgaacgca cgtcgacgcg attgaccacg cgcgaaccga ccacctcgac
    13261 cgcggccagg ttggcgacgc caccttcgct gcccaggttg accgtggtgg tgccgccaag
    13321 cgagacattg acgttcaccg gcgagccggt gcctgcggcc atcgtgtagt cgcccggcgg
    13381 caactggccg atccggtacg tgccgtcgct gttcacggtg acgctgcggc tggcgccggt
    13441 ggcgctgttc accacggtca cctgctcacc gggattcgcg cgtcccgcca ctgcaccggt
    13501 ggcgctctgc gcgaaggcag gcgcggcggc gagcgacgcg aggcaaagcc ccatcgcaat
    13561 gctgagggcg gacttgcgca ggcggcttgg ggcggtgctc atggacgttc tccgggcgaa
    13621 ggcgaggcgg gcgtggatgg gaggaacgcg gcgatggcaa accggcgcgc gccatcgcgc
    13681 gcgccgcggg gcggtggcgt gtgcaaggcg gtgtggcgcg ggtgcatccg gaacgttccc
    13741 caacggtcgg cgatacaccg gctgtgtagc acgcagtgca gggctggtca ataatttatt
    13801 cttcacgtat aagttttgga attagctatt cctcgatgcg ctcccgccac aatgcggcgc
    13861 tgcgcatccg ctcgaggatc cgcatgggag ggaatgcgat gacggaacac cgacgcaacg
    13921 ggatggccct gcccacgctg cgcgcgctgg cggtggcgct tgccctggtg gttgcgcctg
    13981 cctcggcgtt cgacaggacc ctgcaaacgc cgctgccggt gccgccaagc ggcgtcatcg
    14041 ggatgcagga cgcctacttc gcgccggagt tctggatcgc gcgcaccccg gcagcggacc
    14101 aggtgttgat gacgcctgcc gcgatccagg cgcgcaacgc acgcctgctg caggtcgacg
    14161 attcgatgca cgatctgtcg gcattgccag cgaccttgga ccgtgcacgc gtcgccggat
    14221 ggatcgaaga tctggcttcg ccgccgtcga aaccgttgtg ggacgaaacc gccaagccgg
    14281 tgccgaaggc gacgctcgat gcgatcgtcg gcaaccgcgc gatgggcgcg atcccggcca
    14341 gccagccgac gcgcttcggc atggccgtgc agcgcacggc gatgcgcgcc ttcccgaccg
    14401 agctgcgggt gttcaatcgc cagggcgaga ccgacatcga ccggttccag gaaagcgcgt
    14461 tgttccccgg cgatccggtg gcgatcgtgc acgccagccg tgatgcgaag tggctgttcg
    14521 tggtcagcga acgctatgcc gcgtgggtgg cggcaacggc catcgcggaa ggcggtcgcg
    14581 acgcggtgct gggatacggt gcccgcacgc cctaccggat cgtcaccggc gccaagccgc
    14641 acacggtgtt cacccgcgag gaaccgcgcc tgtccgagct gcaactggac atgggcgtgc
    14701 gcatcccctt ggcgaacgtt gcgctggaca agccggtgaa cggccagcat ccctatgcgt
    14761 cgtggatcct cgacttgccc gtgcgcggca gcgacggccg gctcggcttc gcgcccgccc
    14821 tgcttccgcg caatgcggac agcgccgcgg actacctgcc gctgacccgc gccaacctga
    14881 tccgccaagc cttcaggttc ctcggcgagc gctatggctg ggggcattcg tacaacggcc
    14941 gcgactgcag cggcttcgtg tccgaggtct accgcagcat gggcgtcgag ttgccgcgca
    15001 acaccagcga ccagtcggtc agcccggcct tcgaccgcat ccatttcgaa accgacacgc
    15061 ctcgggccac gcgcatggct gcggtcgatg cgctggacgt cggcgacctg atctacatcc
    15121 ccggccacgt gatgatgttc gctggccgca tcgacggcat gccgtgggtg atccacgaca
    15181 ccaacggcgg cagtttcctc ggcgccgatg gcacactgca gcccatgcaa ctcaatggcg
    15241 tgtcgctgac gccgctggtc ccgctgcgct tcggcaagga ccacgactac atcgaccgca
    15301 tcaccaacat cgtccgggtg gcgcgcgaca aaatgaagat catccaatga aaataagcga
    15361 gatccgcttc ggcatgctgc gcgtgccgct gaagaccgcg ttcaagactg ccctgcgcac
    15421 cgtcgagcag gtcgaggacg tggtggtgat gatccacacc gacaccggcc acgtgggcta
    15481 tggcagcgca ccggcgaccg cggtgatcac cggcgatacc catggctcga tcatgcacgc
    15541 gatccgccac tacatcgcgc cgcggctggt cggccaggag atcgccaacc tcaaccgcat
    15601 cacccatgcg gtgcaggcct cgatggagaa gaacaccagc gccaaggcgg cggtggagat
    15661 cgccgtccac gacctgtggg gccagttgta tggcgcgccg ctctaccagc tgctgggtgg
    15721 cggcgagccg gcgatcacca ccgacatcac catctccgtc gactacatcg acaagatggt
    15781 ggcggactcg ctggccgcgg tcgatcgcgg cttcgagtcg ctgaagatca aggtcggcaa
    15841 ggacatcggc ctggacatcg aacgcaccaa ggcgatccac gccgcggtcg agggccgcgc
    15901 cctgctgcgc ctggacgcca accagggctg gaccgccaag caggcggtgt atgccttgca
    15961 gacgctggag gacgccggcg tgcgcctgga gctggtcgag cagccggtca aggcgcacga
    16021 cctggaaggc ctgcgctacg tcaccgagcg cgtgcatacg ccggtgatgg ccgatgaaag
    16081 cgtgttcggg ccgatggaag taatcgaact gatccgcatg cgcgccgccg acatcgtcaa
    16141 catcaagctg atgaagaccg gcggcatctc caatgcggtg aagatcgccg acatctgcgg
    16201 cctctacggc gtggactgca tgatcggctg catgctggaa tccagcatcg gcgtggccgc
    16261 ggcggtgcac ctggcggtgg ccaagtcgga cgtgatcagc aagatcgacc tggacgggcc
    16321 gtcgctgtgc cgcttcgatc cggtcagcag cggcgtgctg ttcaacgaat ccgagatcag
    16381 cgtgaccgac gcgccgggcc tgggcatccg cgcgatcgaa ggcctcgtcc tcgtcgacga
    16441 acccctggcc tgaggcgcgc gaaatgtcgg cgctggtcaa gatccgctcg gaacgcgacc
    16501 gcatgtcggc gatcgagcgg cgcatcgccg acttcgtgct ggacaacgcg caactgctgc
    16561 gcgactactc ctcgcagcaa ctggccaacg cactcggcat cagccagtcc agcgtggtca
    16621 agttctgcca gaagctgggc ttcaagggct atccggacct caagctgtcg atcagcgagg
    16681 cggtggtgcg cgccgacaac ggcaacggcg gcacgccatc cgcggcgaac ggcgacaccc
    16741 acgccgagac catcggcgcc gacctgtggc tgcgcaagac cgaggccgag caagccaccc
    16801 gcctgatcaa tccgccggac gcggtcgaag ccgctgcagg ggcgatcgcc gccgccggca
    16861 aggtcttcat catcggcctg ggcgacgacg acatccacgc gcgcaccctc gccctgcgac
    16921 tgtcgctgct cggcatcctc acggtgcaca acttcgatcc cgcgcacatg agtaccagcc
    16981 tgtccgcgac gaatccgggc gacgtcctgc tggtgttctc cgaacagggc atgcagccgg
    17041 cgctgtacca gttgtgccgc tacgcacgcg agcagcatgc acgggtggtc accgtcaccc
    17101 gccacagttc caacccgttg cgcgcgcatg ccgacgtgcc gctgctggtc tccgcgcacg
    17161 acgagcgccc gcacatcgcg ccactgctct accactccgc gctgcagcac ctgctcgacc
    17221 gcgtgttcgc ggtgctgtgc gaatccgacc cgaagcgccg cgcgcgcctg caggcgaacc
    17281 tggaacggat ccgcgaaaag gggtcagagt gaatttcccg tcaaatgatt ttgagcatac
    17341 gcacacgcgg cacgggaaac ttcactctga ctccttttcc cttacgatcc tgcataccct
    17401 cccggacaaa acccgcatgc cgattcgccg tatcgctgcc ctgctcctga ccaccgtgct
    17461 cgctgcctgc gcgcatgcgc cgcaggcctc cactggtacg aacacctggt ccgatgccgg
    17521 gcaactggtg ctggtcacca ccgcggactg ggacgcgacc accggtgaac tgcgccgctt
    17581 cgaacgcgac gggaccggat ggcgcgaagt cggcgaagcc gcgccgatca gcgtgggccg
    17641 taccgggacg gcctggggca tcggcctgaa tgccgcgcgc agcgatggcc cggtcaagcg
    17701 cgaaggcgac ggcaaggcgc cggctggcgt gttcgcgatc ggcaccgcct tcggctatgc
    17761 cgcaggggcg accaccggcc tggaatacaa gggcatgggc ttcaacgatt ggtgcatcga
    17821 cgtgccggag tcgccgctgt acaaccggat cgtcgaccgc agcctcgcca aggcgcctgg
    17881 cctggacaaa tcgagcgaac cgatgcgcct cgacctgcac gcgaacggcg accagcgcta
    17941 ccggcagggc ttcgtgatcg agcacaacgc caatggcacc accccgcgcg gcggcagctg
    18001 catcttcgcc cacctgtggg gctcgccggg ccaggccacg gccggctgca ccgcgatggc
    18061 gccagagacc atgcaaccgc tgctggcctg gctcgaccgc aaccgcaagc cggtgttcgt
    18121 gctgctgccg aaggcgcaat acgccgcgtt gaaacacgac tggaacctgc cggagatcgc
    18181 gcgatgagcc agaccaagcg cgtcctgagc ggcctggccg caggcatcgt cgtcggcatc
    18241 ctgctggcgt ggctggatgc gccgctggcg ttgcaggtcg cggacatcgt cgagccgttc
    18301 ggcaagctct ggctcaacgc cctgcagatg accgtggtgc ccctggtgct ggcgctggtg
    18361 atcgtcggcg tgaaccaggc cagcgatgcc gcccagtccg gccgcgtcgc gcggcgttcg
    18421 atcatcatct tcgtgatcct gctcaccgcc gcgggcacga tggccgcgtt gctcgcgccc
    18481 ctggcgctgt cgctggtcgc gccggatccc gcggtcgccg ccgcgttggg cggaagcagc
    18541 gtggaactgc ccgcgacgac cggcggctgg gccagcgcgc tgacctcgat cgtgccgagc
    18601 aatgcggtgg ctgctgctgc ggccggcgcg atgctgccgc tggtcacgtt tgcgctgttc
    18661 ttcggcttcg cgctgacccg catcgaaccc gcgcgccgca cgcaagtcgt cggcttcttc
    18721 caggcagtca gcgacgcgat gatcgtgatc gtgcactggg tgctgtgggt ggcgccgttg
    18781 ggcatcttcg cgctggtgct ggcgctatgc gcgcgcgccg gattgggcat gctgcaggcg
    18841 ctgggcatct acatcctgct ggagtgcgcg ttgtatgcgc tggcggtggt cctgctggtg
    18901 gtcatcgcgg tggtgttcgg caagcagaat cccggccact tcctgtccgg catcacgccg
    18961 gtcatggccg tcgcagccag cacgcaatcg tcgctggcca ccctgccggc gatgctcgac
    19021 agcgcgcaga cgcgtctcgg tttccccgcg cgcatcgccg gcctggtgct gccgatggcg
    19081 gtctcgctat tccgcatcac ctcgccgatc cagtatatcg ccaccgcctg cttcatcgca
    19141 tgggcgctcg gcatcgacct gaccttcgcc catctcgcca ccggcgtggc cttgtcggtg
    19201 ctggtcagca tgggctcggt cggcctgccc ggccaggcaa tcttcctggc caccaatctg
    19261 ccggtcagct cggcgatggg cctgccgatc gcacccctgc cggtgctgat ggcggtggac
    19321 gcgatcccgg acgtgctggc cacggtcggc aacgtgaccg cggacatgac cgcgaccgcg
    19381 ctggtggcgt cacgggacga tggcgatggc gaaccggttc cacccgcaac caccgcttga
    19441 caggctccgc tgcgctgcag cagagtccag acccatgtcc cgccgcatga ccaccactac
    19501 gaccattacc accgagacta tttcggtgcg gtcggtcatg cctggataac cccatccaat
    19561 cacggccccg cagcgacaga ggcgggggcg acgatcccct cccctgcgcg ccgcgggcct
    19621 ccaccggagg ttccatggtt tcgtcgcagg ttgcgcaatc cccgtcgtgt cgcgcgcaca
    19681 agttcggcgg cagttcgctg gccgacgcca gccgcatccg ccatgtcgcg tcgctgatgc
    19741 tgggcgcggg ggaaccacgc caggtcgtgg tggtgtcggc catgcagggc accaccaatg
    19801 cgctgatcgc catgacgacg gcggccgcgg ccggcgagga ctggcaagcc gcatggtcgg
    19861 cgctgcgcac gcaacacgcc gatgccgcca gcgccctgct cgaggcacct gccgaactgc
    19921 acgcgtggat cgacgctcgc ttcgccgaca tcgccgacct gctgcgcgcg gtgtcgctgc
    19981 tgcgccagcc cggacgcgcg gcgctggacc gcatccaggg catgggtgag gtgctgtcag
    20041 ccacgctgct gcaggcgcac ctgcgcgcac gcggcgccga ctatgcgctg ctcgacgcgc
    20101 gcgacgtgct gctggtccgc catgccgaac tcggtgccgt cgtcgattgg gacgaaagcg
    20161 cgcgcaggct cgatgcatgg cgtgccgcca acccgcagcc gcgcgtggtc gtcaccggct
    20221 tcgtcgccgg tggcgaagac ggcctgccca ccgtattggg ccgcaacgga agcgatttct
    20281 ccggcgcgat cttcgccgcc ctgttcgatg ccgccgagct gcacatctgg tcggacgtcg
    20341 acggcgtgct ctcggccgac ccgcggctgg tgccggaagc ggtctcgctg gcgtcgatga
    20401 gttatcgcga agcctgcgag ctcgcctact tcggtgcgaa ggtgatccat ccgcagacca
    20461 tgaccccggc gatcgcgcgc ggcctgccga tcctgatgcg caacaccttc aagcccgagt
    20521 tccccggcac ccgcatcgat gccgatggcg atggcggcca tggcggaccg gtgaagggac
    20581 tcacgctgga tcccggcctc gccttggtga gcctggaagg cgccggcctg atcggcgtgc
    20641 ccggcacggc ggagcgtgtg ttcgcggcgt tgcatgcggc gcgcatttcg gtggtgatga
    20701 tctcgcaggg cagctccgag cattcgatct gctcggtggt gcgcgagcgc gatgccgaag
    20761 ccgcgcgtcg cgccctgctc gacgcgtttt cgcgcgagct cgacggcggc caggtgcagg
    20821 gcgtgcaggt cgttcccggc atcagcgtgc tggccgcggt cggcgacggc atggccggca
    20881 agcccggcgt ggccgcgcaa ttgttcgcgg ccctggcgcg tgcgcgggtc aacatccgcg
    20941 ccatcgccca aggtgcgtcc gagcgcaaca tctcggtggc catcgccgcc gaagacgcca
    21001 cccgcggctt gcgtgccgcg catgcggcgt tctggctatc gccgcagacg ctggcgctgg
    21061 cggtgatcgg cccgggcaag gtcggcgccg ccctgctcga tcagttgcag gcggcgttgc
    21121 cacgcctgcg cagcgaagcc aacgtcgacc tgcgcctgcg cgcactcgcc accagccgcc
    21181 gcatgtggct ctgcgaccac ggcgatcctg ccgactggcg cacgcgcatc gcggaagaac
    21241 cgcagcccgc cgacctcgac gcgatcacca cccacctgct ggacagtcac ctgccgcatg
    21301 cggtcgtgat cgactgcagc gccagcgaca gcgttgccgc gcagtacccg gcatggctgg
    21361 cggccggcat ccacgtcatc accccgaaca agcatgcggg cgctggccca ctaccccgct
    21421 acgcggcaat ccgcgaggcc cgcgcgagca gcggcgcacg cttccgctac gaggccaccg
    21481 tcggtgccgg cctgccggtg atctcgaccc tgcgcgacct gctggacacc ggcgatgaac
    21541 tggtcgcggt cgacggcatc ctctccggca ccctggcctg gctgttcaac aagttcgacg
    21601 gcagcacgcc gttctccgac ctggtgcgcg aggcgcatgc gctgggctac accgagcccg
    21661 atccgcgcga cgacctgtcc ggcatggacg tggcgcgcaa gctggtgatc ctgggccgcg
    21721 aagccggcat ggcgctgtcg ctcgaggatg tgcgcgtgca gggcctggtg cccgaggccc
    21781 tgcgcgacac cgatcgcgac agcttcatgg ccgggctggc atcgctcgac gcgccgatgc
    21841 aggccgcgct ggaggccgcc cgcgccaggg gctgcgtgct gcgttatgtc gcgcgcttgc
    21901 agcgtgatgg caacgcgtcg gttgcgctgc aggaactgcc cgccgaccat gcctttgccc
    21961 acctgcgact gacggacaac tgcgtgcaat tcaccactcg ccgctatcgc gacaacccgc
    22021 tcgtcgtgca gggccctggc gccggtcccg aggtcaccgc cgccggcgtg ttctccgacc
    22081 tgttgcgcct ggccgccgca ctgggagcgc gcctgtgagc ggacgcatgg aagcacgcgc
    22141 gttctcgccc ggctccgtcg gcaacgtcgg cgtcggcttc gacatcctcg gccacagcat
    22201 cgccggcatc ggcgacatcg ccacggtgcg tcgcatcgat gagccggtcg tgcgcatcaa
    22261 ggcgatccgc ggcgcggtca ccgacctgcc gctggaggcc gaacgcaaca ctgccggtgg
    22321 cgcgctcatc tcattgcgcg aggcgctggc gttgccgttc ggcttcgaga tcgaactcga
    22381 caagggcatc ccgctcggtt ccgggctggg tggctcggcg gcgtcctgcg tggctgcact
    22441 ggtcgcggcg aatgcgctgc tcgacgcgcc gctcgatgcg catgcgctgt acccgcacgc
    22501 catggccggc gaagcgatcg ccagcggcgg acggcacggc gacaacgtcg gcccgatgct
    22561 gcttggcggg ctggtgctgg cgacggccga ccgcctgacg aagatcgcgg tcccggccgc
    22621 gtggcatgcg gtggtggtgc atccgcatgc ggtgctggag acgaaacatg cgcgcgccgc
    22681 actcgccggc agctatgcgc tcaaggaatt cgtggcgcag agcgcgcaac tggcgcaggt
    22741 gctgctgggt tgcgagcgcg gcgacgccgc gctggttcgc gccggattgg acgatgtgct
    22801 ggtggagcca cgtcgcgcgc cgctgatcgc aggcttcgcc gcggtgaaac aggcggcgct
    22861 cgatgcaggc gcgatgggcg ccagcatttc cggcgccggc cccagcgtgt tcgcctggtg
    22921 cgaggatgag gcgaccgcgc agaccgcggg cgcgtcgatg gcagcggcat tcgcatcggc
    22981 tggcttcgac agcgacgtgc atgtcggccc ggtcgcgggc cccgcggcag gggtgctggc
    23041 atgaaggtgc tgagcacgcg tggcatcgca tcgcctgtgg cgatcagcca ggccatcgcc
    23101 gccggcctcg cccccgatgg cggcctgtac atgccggaac actggccgac gttttctccc
    23161 gccgatttcg ctggcgacga gaccctggcc gctgtcggca agcgattgct ggcgccgttc
    23221 ttcgccgacg atgcgatcgg cgaacggctc gacgcgattt gcgatgacgc gttcgcggtc
    23281 accccaccac tgcgccgctt cgggaccggg gacgacttcg tgctggagtt gttccacggc
    23341 ccgaccgccg cgttcaagga ctacggcgcg gccttcctgg ccggctgcct gggcgcattg
    23401 ccacggggcg aggtgccgct gacgatcgtc gtcgccacct ccggcgatac cggcgctgcg
    23461 gtcggcgcgg cgttccatcg caagccgggc ttccgcgtcg ccatcctgta tccggatggc
    23521 cgcgtctcgc cgcggcaggc ccaccagttg ggctgcttcg gcgacaacgt gcgcgcgttc
    23581 cgggtggatg gcagcttcga cgattgccag cggctggtga agtcggcgct ctcggacgcc
    23641 gagttccgct cagaggtgcc gctgggctcg gccaacagca tcagcctggg ccggctgttg
    23701 ccgcagatga cgtactacgc gcatgcctcg ctgatgcact ggcgcaagca tgcaacgacg
    23761 ctgaatttcg tcgtgcccac cggcaacctc ggcaacgggc tggcctgcgt gctggcgcgg
    23821 cgcatgggct tgccgatcgg cgagatcgtg ctggccagca acgccaatgc ggtgctgccg
    23881 gacttcttcg ccggcggcga ctacacgccc ggcccgagca tcgccaccct cgccaacgcg
    23941 atggacgtgg gcgcgccgaa caatttcgag cgcctgcaat ggctgtatcc ggatgccggc
    24001 gggctgcgtg cgttgctgtc cgctgacgcc gtcgacgacg ccaccatccg ggccaccatc
    24061 gtcgatgcct ggcaacggca aggcgaggcg atctgcccgc ataccgcgtg cgcggtgcac
    24121 gtcctgcagc gcaggcgcgc ggccggcgcg agcggcgact gggtggcggt ctcgaccgcg
    24181 catccggcca agttcccgga ggtggtcgaa ccgctgatcg gccaggccat cgccctgccc
    24241 ccggcactgg cggcgatgct cgaacgcccc agccacgcgc aggcgctggc cgatgatggc
    24301 gaggccctgc gcgatctgct caggcatggc tgaggtggtt cgggcattcc gccaagatcg
    24361 cccggtcgga tcgccaatcc agccgtcgca ttggccgaaa ccccgaccgg ctgcgataat
    24421 cgtcacgctt tggccccgta gctcagctgg atagagcggt cccctcctaa gggacaggtc
    24481 gtgcgttcga atcgcgccgg ggtcaccacc ttccctaccg cgcccccggc ttcccgtggg
    24541 cgtggccgca ttggttccag gagttccgca tgtcccatcc cgtccttacc gcgctcggcc
    24601 tgcaggccgt cgagtccggc acaacaatct ctggcacata tgttggccat ggcgaatggt
    24661 cgaagaccag cgacgccggt gtcatcgaac cggtcaaccc gaccaccggc gaggtgctgg
    24721 gccgcgtgca tgcgtcgtcg caggcggact acgacctgat cgtcgagcgt gcccaggccg
    24781 cgttcaaggt ctggcgcacc accccggccc cgcgccgcgg cgaggcgatc cgcctgtgcg
    24841 ccgacgcgct gcgcgtgcac aaggacgcgc tgggctcgct ggtcgcgctg gagatgggca
    24901 agtcgaagcc ggaaggcgac ggcgaagtgc aggaaatgat cgacatcggc gagttctccg
    24961 ttggcctgtc gcgccagctg tacggcctga ccatgcacag cgaacgcccg ggccaccgca
    25021 tgtacgagca gtggcatccg atcggcatcg tcgggatcat cagcgcgttc aacttcccgg
    25081 tcgcggtgtg ggcctggaat gcgttcgtcg cggcgatctg cggcgacatc agcatctgga
    25141 agccctcgcc gaagaccccg ctgtcggcga tcgcgtcgat gaagatctgc aacgaggcac
    25201 tggccaaggc cggcttcccg gacatcttct tcctgttcaa cgatgccggc agcgacctgg
    25261 cgcagtcctt cgtggacgac aagcgcgtcc cgctgatcag cttcaccgga tcgaccaagg
    25321 tcggacgcat ggtcggcgag cgcgtggcgc gccgcatggg ccgttccttg ctggaactgg
    25381 gcggcaacaa cgcgatcatc gtcgacgaaa ccgccgacct gaagctggcg atcccggcga
    25441 tcgcattcgg cgcggtcggc accgcaggcc agcgctgcac caccacccgc cgcctgatcg
    25501 tgcatgaatc catctacgac gacgtgctgg ccaagctggt cgccgcgtac aagcaggtcg
    25561 agaagaagat cggcgacccg accgacccgg ccaacctgat gggcccgctc aacagcaagg
    25621 acggcgtgga tgcctacctc gactcgatcg ccaaggccaa ggccgcgggc ggcaccgtcg
    25681 agaccggcgg cgaagcaatc gaccgcccgg gcaacttcgt gctgcccgcc atcgtcaccg
    25741 gcttgaccaa cgaggccgag atcgtccagc acgagacctt cgcgccgatc ctgtacgtga
    25801 tgaagtacaa ggacctggac gaagcgatcg agatgcagaa cgcggtgccg cagggcctga
    25861 gttcgtcgat cttcaccacc aacctcaagc gcgcggaagc gttcctggcc gccagcggtt
    25921 cggattgcgg catcgccaac gtcaacatcg gcaccagcgg cgcggagatc ggcggcgcgt
    25981 tcggcggcga gaaggagacc ggcggcggcc gcgagtccgg cagcgatgcg tggaaggcct
    26041 acatgcgccg gcagaccaac accatcaact attccgactc cctgccgctg gcgcagggca
    26101 tcaagttcga cctctgagga cccgaccatg aatgccatgc cccgccagac cagcacgctc
    26161 gccatcatca gcctcgtcgc cggcatcctc ggctggacgg tggtgccgtt cttcggcagc
    26221 atcgccgcgg tggtctgcgg gcacatggcg cgcaaggaaa tccgccttgc gcccgatcgg
    26281 ctggaaggcg acggcctggc gattgggggc ctggtgctgg ggtatctgtc gatcgcgatg
    26341 gcggtgctcg tcgtggtggc gatcttcctg ttcttcggcg gcctggccgc cttcctcgcc
    26401 ttcgccaacc actgacgcct tgtacgcgct cagccgcccg ttcctgttcg ccttcgatgc
    26461 cgagcgtgcg cacggcctcg ggctggcgtc gctggaggcc gcctaccgca gcggcctgaa
    26521 cccgctgatc gcgaagccgc cgaaaccgct gccgaccaag gcgttcgggc tgaccttccc
    26581 gaacccggtt ggcctggccg ccgggctgga caagaacggc gcgcacatcg atgccttgct
    26641 cgcgctcggc ttcggcttcg tcgaggtggg cacggtcacc ccgcgtccgc agccgggcaa
    26701 cccgaagccg cgcatgttcc gcctgccgcg acaggaagcg gtgatcaacc gactgggctt
    26761 caacaacgag ggcgtggacg cgctggtgcg caacgtcgaa cgcgcgcgcc ggatcggtgg
    26821 cctgctcggc atcaacatcg gcaagaacaa ggacacgccg aacgagtcgg cggaagatga
    26881 ttacctgcac tgcctgcgca aggtctatgc gctggccgat tacgtgaccg tcaacatctc
    26941 ctcgccgaac accgcgggac tgcgcgaact gcaggaagag caggcgctgc gtcgcctggt
    27001 cagcgtgttg cgcgacgagc aggagaaact cgccggccag cacggcaagc gcgtgccgat
    27061 gctggtgaag atcgcgcccg acctgtccga gagcgacatc gatgcggcgg gccgcgtgct
    27121 cggcgacctc gcggtggatg gcgtcatcgc caccaacacc accatcgccc gcgacggcgt
    27181 ggaaggcgcg cgcttcgccg acgaagtggg cggcctgtcc ggtgcgccgc tgatgggcaa
    27241 gtcgaccgcc gtgctgcgga tgctgcgaac gcgcctgccc gaatcgatcc cgctggtcgg
    27301 tgtgggcggc atcctgcacg gcgccgatgc cgcgaccaag caggcggcgg gtgcgctgct
    27361 ggtgcaggtc tacacgggac tggtctatcg cggccccgcg ctggtcggcg aatgcgtgga
    27421 tgcgctgcgc cgtcgcaagg aagcgccaag cagcacaccc ctgccgctgg catgaccgcc
    27481 ggctatcgca tcgtcgaaaa cgcgcggctg gatgcgcgca ataccttcgg cgtagcggcc
    27541 cgtgcgccca tgctggtcga cgtggccgat gccgccgcgc tgccggaact gttcggctac
    27601 gccatgctcc gcgatggccc ggtgctggtc ctcggcggtg gcagcaacct gctgttcgcc
    27661 ggcgatgcgc ccggcgcggt gctggcgctg tcctcgcaac gcatcgaact gctccgcgag
    27721 gttggcgatg cggtgatcgt ccgtgccgat gccggcgtgg gttggcacga cttcgtcctg
    27781 tggacactgg gccatggcct gtgcgggctg gagaacctgg ccctgatccc gggcacggtg
    27841 ggcgccgcgc cgatccagaa catcggcgcc tacggcgtgg aggtgcgcga cgccattcgc
    27901 gccgtgcatg cgttcgatcg ccgcgatcgc ggtttcgtac gccttgatgc cggcgactgc
    27961 gacttcgcct accgcgacag cctgttcaag cacgatcccg atcgttacat cgtcactgcc
    28021 gtcgagttcg tgctgtcgcg cacgccggac ctgcgcctgg agtacgccgg catccgcgag
    28081 gaactgacgg caatgggcat cgacggcacg ccgcgcgcct cgcaggtggc cgaggcggtg
    28141 atccgcatcc gccagcgcaa actccccgac cccgcgcggt tgggcaatgc cggcagtttc
    28201 ttcaagaatc cgatcgtgcc gatggcgatt gccgaggcac tgcaggccac gcacccggcg
    28261 atgccggtgt tccgcggcgc cggcgacgac acccgcaagt tgtccgcggc ctggctgatc
    28321 gaccagtgcg gctggaaagg ccatcgccac ggcgatgccg gcgtctccgc cgagcacgcg
    28381 ctggtgctgg tcaaccacgg ccaggcgacc ggcctgcagt tgctcgacct ggcacgcagg
    28441 atcgccgcat cggtgcggga tcgcttcggc atcgccatcg aacccgagcc gcgcatcatc
    28501 ggcgcggcgt ggtgagcagc gcgcgcgcac cgcacctgcg cgccgcattg ctgatgctgg
    28561 ccagcaccgt cgcgttcggg ttgatggcgc tgtgcatccg cttggcctcg cagaccgaac
    28621 ccacctggga agtcgcgttc ttccgcaatg ccttcggctt cgccgccctg ctgccgatgc
    28681 tggcgtggcc gctgctgcgc agcccgcgcc cgctggcgca gttcgccgcg aacgtgcgca
    28741 ccgaccagtt accgcgctac ttcgttcgct gcgcgatcgg catcgtcagc atgtattgcg
    28801 gcttctgggc gatcggacac ctgccgttgg cgcaggcgat ctcgctggcg tactcatcgc
    28861 cgatcttcat caccatcgcc gccgcgctgt ggctgggcga acacgtgcgc atgcgccgct
    28921 ggcttgcggt cgcggcgggc ttcgtcggcg tgctggtgat cgtgcgcccg tggtcgcatg
    28981 cattcaccac cggcagcctg gtcgcggtcg ccgcggcgat gatgaacgcg ctggtggcga
    29041 tccagatcaa gcagctgtcg cgcgtggaca aggccgacac catcgtgttc tggacctaca
    29101 tcctgtgggt gccgatgtcg ctgatccctg ccctgttcgt ctggcatccg ccgcagggca
    29161 tcacctggct gtggctggtg ttgtgcgggg tgctgggcac cttcggccag ttgctgtgga
    29221 cgcgggcgct caagctgggc gaagtctccg cgctgacgcc gatcagtttc gtgcaactgc
    29281 cgatcgtgac gctggccggc tggctgctgt tcgccgaacg ggtggatgcg atgacgatcc
    29341 tgggggccgc gatcatcctg tcggcgacgt tctacatcgc ccaccgcgag gccgtgctgg
    29401 cgcgcaaggc ggcgtcgcag gcgccgagcg aggccgctgt tcccggcgag tgacgggcta
    29461 cttcgccgcc gtccgcgcgg cgcgggcttc ctcgcgctcg gtctcgatga tgtccttgcg
    29521 cttggccgcc tgctcttcac tcaggctgtc ttccttcgcc tccagcagcg gcgcgatgta
    29581 gcgcgcccgc agggcatcgg cctccttgcg gtttcccgcg ctgcgcagca tgccctccag
    29641 ttgccaggcc gcatcgacgg tgcgggcatc gtccgcacca agcgcccctt cgcggatctc
    29701 cagcagggtg cgccacgagg ccatcgccag cgccttgtca cccagttcgt ccgccgcctg
    29761 cgcgcggatg tcgaagccca tctgcacctg cggatgcttg tcgccgagtt gcttgcgcgc
    29821 caccggcagc cagcggtcga gttgcgccag cgccgccttc ggctgaccgg cctggtagag
    29881 cgtcgctgcg cgattgagct ggatgaacag cgtgtccgga tggtcggggc cgagccggcg
    29941 cgtgcgtgcg tccgcgactt gctgctgcaa ggcgatcgat tcgtcgaagc gcttcagccg
    30001 cgcgtagatc gtcgacaggt tgagcttgga acgcagggtc tgcgcgtctt ccgcgccgag
    30061 cacgcgcgtg cgcgcctcga ctaccgcttc ggcaaccggc aatgcttcgg cgggcttgcc
    30121 gctgtcgttg agcatgttgg ccaggttgcc gcgctcgccc agcgtctgcg ggtgctcggc
    30181 gccaagccgg cgcacctgcg tctgcaccag tccctgctgc agggcgaccg cgccgtcctt
    30241 gtcgccggtc atgatccgca tcaccgccag gttatgcagc gaggccaggg tgtcggcatg
    30301 gcccggaccc agcacccggg tccgcaacgg caccagcctc tccatcagcg cgcgcccttc
    30361 cttcgcttcg cccatccgcc ccgtgaggat cgccaggctg ttggtgacct ccatcgtggt
    30421 cgggtcgcgc tcgccgcgca gctccaggct gtcggcacgc accgcttcca gcagcgcccg
    30481 acccttgggt cgatcaccga gcgaagccac ggcttccgct tcgttgagcc gcaacttgat
    30541 ccgcagcgca tcgctgggca gcaatgcagc cgcatcgacc agcgatgcct gcaccagcgc
    30601 cagtgcaggc ttggcctcgc ccgcttccag catcgcgctc acctgcgcct gccgtgctcg
    30661 caaggtctcc ggcgcggcac gacccagcgc gccggcgcgg taatcggcca cctgcgcgaa
    30721 accgcgtgcc gcgtcccttg gcaggcccaa cgcgacgcgg acgcgggcga cggattcgcg
    30781 caggtcggcg gccagcgccg gctcgcgggc gaagtcgcgg tcgatcgcgc gttcggcgtt
    30841 gaccaggatg tcgcggtcga tcaggccgcg ggcgatgtcc gcggtgctgg cctggcccag
    30901 ggcacgctcc agcgccgcca ggtcggcgac gtcggcggag gcgagttgct tgcgcaagcc
    30961 atcggccatg ccgatcccca tcgcctcgat gtcgatgccc tgcagcatcg actgctggaa
    31021 cgccgccacc ttctccagtt ccacgctgcg ctgctcggcg atggcgcgtt gcgcgcgcgc
    31081 ctgcagcagc ccgtacaccg acaacgccag tccgccgacc agcgccagcg cgaccagtgc
    31141 cgccgccgcg atgccggtgc gatggcgttg cgcgaacttg cgcagtgcat acccgcgcga
    31201 tggcggcacc gcctgcagcg gccgaccgtc caggaagcgc tgcaggtcgt cggccagcgc
    31261 cgccgcggag gcataacgct cgttgcggtc gtggcgcatc gccttcgcca ccacccaatc
    31321 cagctcgctg cgcagcaccc ggcgcatccg cgacaccggt tcgccatgcg ccttcgccag
    31381 gcgctcggcg tcggccgggg agagcgtcga caactgcgcc gacggcaggc gcagggtgcg
    31441 gtcatggtcg gtgacggtct cgcccgcggc cagcgggcgc cgtccggtca acagttcgct
    31501 gaggaccacg cccagcgcgt acacatcgct gcgcgtatcc acccgcgatt ggtcgccacc
    31561 ggcctgttcc ggactcatgt agtcgggtgt gccggcgacc tcgcgcccct cggtcagcga
    31621 cgtcgcggtg gcgatgccga aatcgatgat cttcggcagc gcgcgcccgt cgacctcgtc
    31681 caccagcagg ttgcccggct tgaggtcgcg atggatcacg cccttctggt gggcgtgctg
    31741 cacgccctcg caaacacgga tgaacaacgc gatgcgcgca tcggtcggca tcgcatgcgc
    31801 atcgcagtac gcggtgatcg ggctgccggc gatgaactcc atcgcgaaaa acggatgccc
    31861 gtcggcggtg gtgtcggcat cgaagacctg ggcgatggcc gggtggcgca tctgcgccag
    31921 caactggcgc tcgacctcga aataggccag gtggcgggca tccagtttct gcgcgcgcag
    31981 cagcttgatc gccaccgtgc gccgcaccgg ctccagttgc tcggcgcgat acacctcgcc
    32041 catgccaccg cggccgagca ggccgtcgat gcggtaacgc ccaagccgcg cgcctgacgg
    32101 cagcgcggcc gcctcgccgg tcgttgcacc ggcgaccggc gagcgcgtcg ccagcagttc
    32161 ggtgcgttcc ggatcgttgt gcggaccctg cgccatgcgc ccccctttcg cccgattcta
    32221 gcgaacgcgc ggcacgcgct tgtgcaggcg ctacactgca ggcgactgtt gacgctaccg
    32281 ccgacggaat ggaaccggat accgagctgc aggacaccac ccagcgcacc gtgctgagcc
    32341 acggaccctt gcggtccgcg ccgcgctccg cctgcgtggt ggtgatccat ggcgaaggcc
    32401 tgggccgacg cgccgacatc accgacaccg ccgtcctggt cggccgctcg caggaagccg
    32461 acctggtcat cgcccacaag agcgtctcgc gcgagcattg ccgggtctgg cgcgagggca
    32521 acggctaccg catccgcgac ctcggcgcga ccaatgccac ccgcgtcaac gacgtccgca
    32581 tcgccggcga aaccacgctc gcggacggcg accaggtcac cgtgggcgaa agcatcctga
    32641 agttcatcag ccaggacagc gtggaggcga gttaccacga ggagatctac cagctcgcca
    32701 cccatgacgc gctgaccgag ctgtacaacc gccgtcattt catcgagatg gccgacaagg
    32761 aaatcgcgcg ctcgatccgg catgcgcggc cgctgtcgct gtgcatcatc gacgtcgacc
    32821 tgttcaagcc ggtcaatgat cgctacggcc acatctccgg cgacgaagtc ctgcgccaga
    32881 tcgccctgct gctgcgccgc catgtccgca gcgacgacat cgccgcgcgc atcggtggcg
    32941 aggagttcgc cttgttgctt ccggaatgcg atgccgaggc cgcgcgcgtc ttcgccgaac
    33001 gcctgcgcga ggccgtcgcc gccgccccct tcgcacccgg cggcgaaccg caacggatca
    33061 ccgtgagcat cggcatcgcc gagctggccg cggagcgcga cacccgccct gcgttgatgg
    33121 ccgccgcgga cacggcgctg taccgcgcca agagtgaagg ccgcaaccgg gtctgcgtgg
    33181 aagcctgagc ctcagcgctc ctgccacggc cgctggccgt gcgggaacac cagcaggtac
    33241 gcgccgaccg tccaggcgat ggcgaccgcc cagccggcca ggatgtccga cggatagtgc
    33301 acgcccaggt acacccgcga cagcccgacc atcagcgtga acgcggccaa cggcagggtc
    33361 acccaccagc gccagcgcgt gtgccaggcc agcaacacca ggaccatcgc gatggtgatc
    33421 gagcccatgg cgtggccgct ggggaagctg aacgtgcttt ccggcgcgat cgagtcccac
    33481 agcgcgggcc gctggcgcgc gaacagctgc ttcgcgccca ggttgagcag cgccgagccg
    33541 atgatcgcga cgccggcgaa cagcccctcg cgcagcttgc gcatcgccgc cagcaccagc
    33601 acgaccagga catccagggg caccacgccc cattcgtagc cgagcctgga aaacaccagg
    33661 aaggtgctgt cgaagccgtc gtgggcaacc gattgggcga actgcaggat cggcgcgtcg
    33721 aacgggaaga cctcggcttc gcggacttcg tcggccagct cgccaaaggc ccagagcggc
    33781 agcagcaagc ccgcgaacgc cagcagcaat cgcctccagc gctgggcgat gaaggcgatg
    33841 ccgaagcgcg cgtcgtcgcg cgcgacatcc atcgcctccc tggcggaatg cggcaggcgc
    33901 ggatcatggg gaagcgtcat gcgtgcggcg atccgtcggt catggcagcg gcggatcata
    33961 gccaccgcgg cgtggaggcc ggttcacgac gccgcgcgtc agaggtagtc ctcgatgcgg
    34021 accgcgtcga cctgctcggc gcgcatgaag cgctgcgcgt agtcgtggtg cacgccgctg
    34081 cgcaggaaca cctcgaacaa ctccgggtcg atgtgctggt cctggcgcat cttcgccatg
    34141 atcgacaacg cctctgacag ggtcttgccc ggcttgtacg ggcgatccgc ggcggtcaac
    34201 gcctcgaaga tgtcggcgat cgccatcatc cgcgccagcg ggctcagctc gtcgcgcgcc
    34261 aagcgtttgg gatagccggt gccgtccatc ttctcgtggt ggccgccggc gatctccggc
    34321 accgcgcgca ggtggcgcgg gaacggcagc tcgccgagca tcacgatggt ctgcaccatg
    34381 tgctcgttga tcttgtagcg ctcctccgcg gtcagcgtgc cgcggctgat cgacaggttg
    34441 tgcagttccc cgcggttgta cagcagctcc ggcacgtcca tgcggaagcc ccagcggttg
    34501 tccgcgggga tccgttcgga ttccggccgg gcgatgcggt gttcgggctt gtcggccagc
    34561 agggtctcct cggccggcag cggcatcgcc tgtggcggct tgcgcgccag ctcatcgcgc
    34621 gacaggccga tgcggtcgtc cagggtgcgt tgccagcgcc gcgcgccgat ctcgcgcagg
    34681 cgcgcctgct tgtccggcga gaggaattcg ccgccgacat tgcattgggc aacgaacgcg
    34741 aactcgtcgt cgagggtggc ctgcagcgtg tcgcgcgcct gccgcagcga tgcttcgtcg
    34801 ccgccttcgg cgacgccacg ccagtagtcg acatcggcat cgcgcttgag cacctcgaag
    34861 cgcgtgcgga tctcgtggat gcgatcgttg accgtctcca gcttggtcgc cttgtcgacc
    34921 acgtactcgg gcgtggtgac cttgccgcag tcgtgcagcc aggccgcgat gtgcagggct
    34981 tcccactgtt cggcatcgag gtcgaagccg gcatacggtc cgtcctgcgc cgcgcacgcg
    35041 gcgcgggcca gcatgaaagt caactccggc acgcgttcgc agtggccgcc ggtgtacggg
    35101 ctctgcgcat cgatggcccc ggcgaccatg cggatgctcg cttccatcag cgccttctgc
    35161 ccacgcagca gttcctgcgt ttccagcgag gtggccgcgc tcgccgccaa cgcagcgatg
    35221 aagctcgcgg cggcctcgtc gatctcgccg tccttgaaca gcaacaacat gcccagcaat
    35281 tgctcgcggc gatcctgcag cggcaaggca agcgtgccga acgccgcgaa ctcgggttcc
    35341 aggccgagcg ttcgcaagtc atcgccggac agatggccgc cgaccggccg catggtcgcg
    35401 cgcgcatccg ccagcaggcg cggcagcgcg tcctgtgcca acacaggcaa gccgcccacc
    35461 gcggtcgccc ggccaccgct ggtgtccatc gcggtggcgc gcaatgcgtc gtcggcggca
    35521 tcgaccaggt acagcacgcc ggcacgcgcg ccggcgatgt cgaccatctt gtcgagcagc
    35581 cagggctgca gcgcctccag gtcgtcctcg gtcgccagcc tgcggttgat ccgcaggaag
    35641 cggcgcaccg catcgcgcat ctggtcgaag gcgccgccga gctggtcgat ctcctgcacc
    35701 ggcgaatgga tcaccgggtg gtcgctgaag tcgaagtggc gcacggcctc ggcttccttg
    35761 gccaaggccg ccagcggcgc ggcgatgcga cgcgacgcca gcaaggccac gaccgccact
    35821 gcgccgagga gcagaccgaa ttcggcgacg ccgggaatcg catccccgaa ccagcgcagg
    35881 accagcccgg tggccagcac caggccgaac gacagcaggg tgatgtgcag gtagagcggg
    35941 acgctggtgc gcttgccgtc catggcgccc cccaaggcgc gtgcgagtcc gaccgagtct
    36001 agcgcgctgc ctcgccgcgg tcgcggtcct tcagcccggc cggcccgcgc ggtaccgggt
    36061 gtcgacgtat cggtcgatca gcgccacgaa ctcatgcgcg atgttgtccc cgcgcagggt
    36121 cacggtcttc tcgccgtcca cgaacaccgg cgccgccggc gtttcgccgg tacccggcag
    36181 cgagatgccg atgttggcgt ggcgcgattc gccggggcca ttcacgatgc agcccatcac
    36241 cgcgagggtc atgttt
//
LOCUS       DAOOCX010000006        34755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_919_length_34755_cov_7.496859, whole genome shotgun sequence.
ACCESSION   DAOOCX010000006 DAOOCX010000000
VERSION     DAOOCX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 34755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34755
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_919_length_34755_cov_7.496859"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..423)
                     /locus_tag="PK399_01070"
     CDS             complement(178..423)
                     /locus_tag="PK399_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11749.1"
                     /translation="MTPHALYLSLGDTPAARAHAYRAWLMSGTDDEELARIRAHMTQE
                     KALGNPRFQTMLEKTLNRPVDLKPRGRPALSKGDGGN"
     gene            complement(474..680)
                     /locus_tag="PK399_01075"
     CDS             complement(474..680)
                     /locus_tag="PK399_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11750.1"
                     /translation="MIDTVHPEDLEAIRLHLSRQHAYGSDRFRQAIEAQLGRIVGPQK
                     IGRPRENRGQSRLLKPANLNLPDL"
     gene            complement(708..1346)
                     /locus_tag="PK399_01080"
     CDS             complement(708..1346)
                     /locus_tag="PK399_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPW11751.1"
                     /translation="MCYRSELGEMARREGCAVHAYVLMTNHVHLLLTPSRPGAVGKAM
                     QSLGRRYVRYINATYCRTGTLWEGRYKASLVGDGDYLMQCHRYIELNPLRAAMVADPR
                     DYRWSSHHALAFGEADPLVTPHPDYLALGARPVERQQRYRAMVMDTVNPEDLEAIRLH
                     LSRQHAYGSDRFRQAIEAQLGRIVGPQKIGRPRENRGQSRLLKPANLNLPDL"
     gene            1290..1829
                     /locus_tag="PK399_01085"
     CDS             1290..1829
                     /locus_tag="PK399_01085"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPW11752.1"
                     /translation="MNGATFSSRHLAEFTAVAQVIGIIEEARLPVVAALDDMLRDAGE
                     VDAERAWHVVACALGGHRLIRRWCSRNQSAPRSCVGKVDSDPGFPTRVSAPRIDPRQY
                     ARCCWLGQCSFPRQEAVMKANVGGFDKWIRIVAGLALIAWALTSGPVWAWIGVVPLAT
                     GLFNFCPLYRLIGFNSCSR"
     gene            complement(1998..2756)
                     /locus_tag="PK399_01090"
     CDS             complement(1998..2756)
                     /locus_tag="PK399_01090"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HPW11753.1"
                     /translation="MTVRHCLLGLALVVAQPAFAEGSCAARTGSATQPLVELYTSEGC
                     SSCPPADRWLSQSLTQGADRLNWLAFHVDYWDSIGWPDRFASPAYSRRQEARVAATGA
                     RAVYTPQVIVGSKTNAPWRNPASFREMLDSQSGPASAGLALRLLPQANGGSLLMLGAA
                     PAGTPSAPAQVWVAQYQDDQVTAVRGGENKGTTLRHDRVVRRLLGPWPMARTALTRSM
                     TIEPLAGPWGLVAFVQDAQGRTLQSLTLPASSCQ"
     gene            complement(2917..3489)
                     /locus_tag="PK399_01095"
     CDS             complement(2917..3489)
                     /locus_tag="PK399_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11754.1"
                     /translation="MTSFEFVFGMISVITSLALTRLLSGGVALYRHADRIRFSWRHGF
                     WTAMALMLLLGNWAAFWQMRGISDWSGSEALVPLLFVSVLYAFCDLVMPEEPRDGSVL
                     DLRAYHEHQGRRYKMLQLAFSVLAILVIARRSPSLLDGLSNSGFALVAATCSIVALRT
                     RRVWLDTAAAVVLVAMSPLFLWMHLRGLSG"
     gene            3620..4075
                     /locus_tag="PK399_01100"
     CDS             3620..4075
                     /locus_tag="PK399_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW11755.1"
                     /translation="MQHVRNVSLAVALALCTGAAMAADDGPSAGACFSQAFLAADADG
                     VAACYAEDAIIWFPGGTMAKGRPAIRDGFAHYFSAMTVKDVRLAEMGHVMGEGGSRTA
                     WGTYEMRMVDKASGAEVVTTGRFTDVSRRIDGRWLYIVDHPSDDPPPAK"
     gene            complement(4126..4566)
                     /locus_tag="PK399_01105"
     CDS             complement(4126..4566)
                     /locus_tag="PK399_01105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006450762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPW11756.1"
                     /translation="MAPRARRLTATLSILLLTACSQPPPYTGGDVAELQRIDVVSGQG
                     ATAVAGDEISVHYTGWLYDQAAADKRGRKFDSSRDRGQPFVFLLGAGRVIRGWDDGVA
                     GMRVGGKRELRIPADLGYGRGGARDVIPPGASLVFEVELLDVRK"
     gene            4725..5210
                     /locus_tag="PK399_01110"
     CDS             4725..5210
                     /locus_tag="PK399_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DnrO protein"
                     /protein_id="HPW11757.1"
                     /translation="MLPLSFLSIPALALAAALVAGPVAAQSGTHAGHDAHAGHVAPAT
                     APQQRWATDAALRAGMHDLREATEALHHYEMGHLDDTQRDNAVQRIDAAIGDMVANCK
                     LKPDADAALHGLLVKFIAGANAARAGRFTKAELGPMEQALATYPQRFDDPEWNRTDGR
                     D"
     gene            5331..5915
                     /locus_tag="PK399_01115"
     CDS             5331..5915
                     /locus_tag="PK399_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11758.1"
                     /translation="MSTGDSPAQVLQENVMSKCHSLTAALVGAWLATAAASASAADPK
                     VEASVTIEGKTIALPHARAFSTGMPFVLIYFAEKPLDGLKYGTAGTDATWSGGAYGTM
                     LRFAPAPDPRDEGKPVQRYVIPEAAAEEDDRVALRSARIGNWQAQWLSQEGVRIESLE
                     SSNGVVRGTLSWRGKAPVSAWSASFSVPLEEGVL"
     gene            complement(6018..6608)
                     /locus_tag="PK399_01120"
     CDS             complement(6018..6608)
                     /locus_tag="PK399_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11759.1"
                     /translation="MHPSPALALFGAASRTHPTAGRAMATRTALATLPLLLSYSLVAC
                     ASNGPQTPATQSASPRDKFGDPAQYEARDLPVTDNDLRTLERAAELLASDSAWNRNDD
                     RRCADDEAANKRSLYCALERASADTYGSHDPARVSDHRRVALQEVRFAVEEATRGREL
                     NHRLMDFNNLPETTLADVQRVVAQATARVRARLSEQ"
     gene            6648..6914
                     /locus_tag="PK399_01125"
     CDS             6648..6914
                     /locus_tag="PK399_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11760.1"
                     /translation="MGESLNPAPDAGQKVPAAPALRTDDGGARPTSLLGTRLRWFLAE
                     FLVVVAGVLVAMGLSGLVDKRRDLQREQVYLQQLSADLGTSERN"
     gene            6923..7477
                     /locus_tag="PK399_01130"
     CDS             6923..7477
                     /locus_tag="PK399_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11761.1"
                     /translation="MAFMQARAEAAARVLHRFWRKDPVLDDSFFLDLSQPRGTRRFRP
                     VLGTAQALGSSGELGLIRSDALRADLLAYVERMKTRLDEIDRYDETYYRPGVAMLNLA
                     PLWRTPENSAVLQYSDDKLRSRPNPFDRVPFPNEPADQLASREVYVGYSNLLTAHRNH
                     AVQYEEMLQETRALRKNVEARIRR"
     gene            complement(7690..9570)
                     /locus_tag="PK399_01135"
     CDS             complement(7690..9570)
                     /locus_tag="PK399_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HPW11762.1"
                     /translation="MPTPPVPSEAEPGRDATPSDGQRFTRALVALTRAVWHPDCSFDS
                     ALAVICEHAADALRVDRVNVWHYERDAQRLRCIHAFSARERAHASDDTLESLSLEGDD
                     YVTGFEDVRALDAADVRLHPSTASSLSELRSYLERHGIQALLDAPVRVEGELLGVICH
                     ECTAGPRLWSHDEITFAGSMGDFVAMAFEIVRRRRAEKEVQHLLLHDATTGLPNRDYL
                     VELLRQRLAAPRAPGDVLAVVHVRIDASGGVALPDDAPTADDVMGQVALRLRQLSSNQ
                     THLARVRADGFAFVLEGSTAQRNAVRLAERCIAAVRGLQWDLKDLLPGATVGIAYADA
                     AADGGARVLMRQAESAAERAREHDKFGYQVFDLEHHDALLKRLRLERALRDAPANGEF
                     ELHYQPEYDATTQAWVAAEALLRWRREGRLLAAAEFIEVLESSGQILSLGRWILHRAC
                     ADAAQWAGAPNQVAPTVRVNVSARQFEEAALVADVGDALAASGLAPERLCLEITETTL
                     MRNLDHALGVLQRLKALGVQVALDDFGTGYGSLVYLRRLPVDVIKIDRSFVDGLPGTT
                     ADVAIVKAVFGLAESLGIEVVAEGVERADQQLALQAIGVRRMQGWLFARAMDQASLCA
                     LL"
     gene            complement(9698..10816)
                     /locus_tag="PK399_01140"
     CDS             complement(9698..10816)
                     /locus_tag="PK399_01140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW11763.1"
                     /translation="MNGSSPQRSSPQGAGRHARGEAPPGGWQAAVERATDDPQLGLIS
                     FFAALIALIVLPFSIYRFAQGQIGVGIIDAVIVIAVSAVGLVAWLPGQTERAGVLMAI
                     VTTTGCLVVSVLFARHGLLWSYAVLTTNFLMTSRRMAMIANVIVLGILGSHPEVFANH
                     IERVVFLSSGLLVSLCAFIFSLQSTRQRQALEHMAIHDPLTQAMNRRAFDIDLATTLQ
                     AHQLSQSPCTLAIIDLDHFKSVNDTFGHEVGDELLVEMVRTLRIHQRQQDRLYRFGGE
                     EFILLMPDTAAHGVQRALDGLREEIFQTLRRPDGEPVSVSIGAATLAPGERGAFWLTR
                     ADAALYRAKNGGRNRVMMADQFDPHAQQVADRRGTVHD"
     gene            complement(10941..11732)
                     /locus_tag="PK399_01145"
     CDS             complement(10941..11732)
                     /locus_tag="PK399_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HPW11764.1"
                     /translation="MTNSLQLGSRGPTVSALQAKLNTMLVPSPRLVADGSFGPKTAQA
                     VKAFQQMKRLVADGVVGPKTAAALGLSLGGSSGPAPARYAPPGAPPPPGAIPPPGGAP
                     PAAGPPGFVDLSVFNVVIEAVIAGYQKIGSRLISWIDSDYVPQFVYDRVAATVNAAVN
                     MVVSPLRAISHQTVPLGQDPAAYVTSRIRDGLARGASSLSAALQPLVGLPVIGIVASG
                     YQRNISGLMGIADVALANLKHNGQSAQAVATRIAAAFDAVARQIG"
     gene            12160..15093
                     /locus_tag="PK399_01150"
     CDS             12160..15093
                     /locus_tag="PK399_01150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW11765.1"
                     /translation="MRRWLLAMLAGIALLVLAEGAFAQVKRNRFQNLDTGKTGGVRAL
                     AEDADGLVWVGGDAGLARFDGRQMAAIAPDRLTGPVNALALQGRVRLWIGGEQGLLRW
                     DLAGERLAEAPCKGMVDGIGQVQVTGDGVLGLSRSGLYRVDADTLACSPVRIAGLPEG
                     QPVERFWQDDRQLWLAIRDRGLWRCALPCAAARPWASALAEERIRWISAAPRDGLYVG
                     THRRGLFRLDRDGAVVDHWWRGDDAPADRAFTTNGVMALQAMPDGRLHAGLWAGGLME
                     VAADGAVVSRSRPLPHEGTSLAGLNVRALLLARNGTLWIGHENGLSLLDPLRNRSAWI
                     GPVAEGQPGVDASGVSAILRDGDMLFVGTGRGGVNRVDLGTGAVTAVQHDPRRRDSLP
                     DDAIWDMADTGDGRLMLATSGGLARLSKDNLRAETLATSPVLPSDDVVTVARARDGGY
                     WLATWAGGVVRVDAAGGVARTWRAADGLDTETMVAVYEDARGRVLASNADGLFLLGRD
                     GRFRTVEVQGTHRRGKPADMLAFHDAADGTLWMGAASGGLARWPAAAERPAWVDEPTF
                     GDIGINAIQARAGGGLWLGSNTGLLAVDAQGRVLARMGVGTGLESEVVTALLGEEGGD
                     LWVGGASGVQRIEPGMASVARQDAAPVISGVRLFNRPLHPDPEGALTASPTHGGALRL
                     RYDQDMLTLDFALPGLPQPVGLRYRYRLQGFAGDWVEVAADEPRAVYTRLPPGDYRFQ
                     VQAGDAGRWSDRIAELPVSVSPPWWMTWWARALLVLAIMAVLASWYRWRTVQLRRQSQ
                     VLEQTVLERTAALREANQALQASARTDALTGLANRRGFQDAVGPRWTALTGNAALLLA
                     DIDHFKAINDAFGHDIGDAVLVDVGKLLDAVARDGDLVARWGGEEFLCLLVGTDARAR
                     AEEMRAAVSAAGPRAALGATRVSITAGYAAIRAGESFQEAVRRADALLYVGKRGGRNR
                     VVFED"
     gene            15224..16003
                     /locus_tag="PK399_01155"
     CDS             15224..16003
                     /locus_tag="PK399_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11766.1"
                     /translation="MYSELKPGPICSTIATLSRRIQERFPGSGLGKVADELLRVAGDN
                     QRVAEQLRGPIWKVRVFTALAVVMLGALASWAVLELARRAAGGIGGVADLLQAADAGT
                     NQLILLSLAVLFLVSLETRIKRAAALRMLHQLRSIAHVVDMHQLTKDPEHALRSVAST
                     ASSPVRTLTRPQLARYLDYCSELLALTSKLAALHAQHLHDPVVLDAVNDIETLTADLS
                     RKIWQKITILDMGEERAVPASGGAGMGPVSAHDANGAGQGR"
     gene            16175..17896
                     /locus_tag="PK399_01160"
     CDS             16175..17896
                     /locus_tag="PK399_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW11767.1"
                     /translation="MGPLDFINAGGEMGAMIRAHDWASSPIGPPEQWPQALKTALRMA
                     LTTRHPIFIFWGPEHICFYNDGYRPSLGPEKHPALLGARGSELFSELWDIIGPQIDMV
                     MRGEGATWHENHLVPSYRHGVLDEVYWTYSYGPIDDETAPGGVGGVLVVCTETTQQIK
                     VANALRESERALKQLNDELEQRVSIEVAERLREQARLAEAQEALRQSQKIETLGQLTG
                     GVAHDFNNLLTPVMGALDFARLELQGDEVAQEVIAVGLQAAERARTLVQRLLAFGRRQ
                     HLQPQDIEAESLMDSAVELAQRSLRPGIRLVLDMPDTLPAVHADPDQVVLAVLNLVVN
                     ARDAIEGVGNITLRVREDNDPGQAGLLPGRYLRIDVCDDGCGMDEAVVARAAEPFFTT
                     KPTGQGTGLGLASVQGLAVQSGGGFKLASTPGEGTTVSLWMPASTKPGIDSQRQGAQR
                     SRTQVAKCHCILLVDDDALVRASGTSSLRRAGYDVVQAADGEEALALLDAAGPFDLLV
                     TDYAMPGLSGLELIDAVRLRKPQMPILMVTGYADTGQEAADVPRLQKPFLPAELVDRV
                     QALIAEA"
     gene            complement(18183..19364)
                     /locus_tag="PK399_01165"
     CDS             complement(18183..19364)
                     /locus_tag="PK399_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPW11768.1"
                     /translation="MSMPSPLRPALLASALLLATASVAASEPQQARTIIVMDGSGSMW
                     GQIDGRPKLEIARETVADVVGKLPANQTLGLIAYGHRRKGDCKDIELIVPPAPGTSGK
                     VLNAVNDMRFLGMTPLSEAVRQAAQALRHTEQAATVVLVTDGLETCNLDPCAVANELE
                     KSGVNFTAHVIGFGLTRDEGAKVACLATNTGGRYLQASDGAALSAALRQTVSEPAPAK
                     PAATLSAPASAPMGSRVAIKWTGPAGNLDTVAIVEQVDGKPTTHDYAYVKDGNPLTLT
                     MPGESGAYTLQYRHRDQTVIATRPIQASPAQAGLKAPDVAQAGSAVVIHWTGPNASLD
                     NIQVAVQGSDSYISYTYLGSDSRQVELTMPEQPGNYELRYVFRDRETIATRPIRVVGK
                     P"
     assembly_gap    19573..19672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19673..21646)
                     /locus_tag="PK399_01170"
     CDS             complement(<19673..21646)
                     /locus_tag="PK399_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPW11769.1"
                     /translation="MRTNAYRACDLCEAICGLELRFEDGALAAIHGDAADPFSQGHIC
                     PKGNAILDLETDPDRLRVPMLREGSAWRAIGWDEAFALAGSRLAAIQQAHGPASVAAY
                     LGNPNVHHFGHIAYLPSLLRMLRTPNIFSASSVDQWPHQFACAQMYGHQFLLPIPDID
                     HTDYFLMLGANPMASNGSLMTAPGIPRRLKALTARGKLVVVDPRRTETAAIASQHIPI
                     RPGGDALFLVALLQAMQQVSPPRLDAYAGRLAGLDIALAAINAIDTASLEAETGIAMH
                     VVEQVAREFLAAPHAVAYGRMGVSVQPWGTLCQVLLQLVNLVSGNLDRVGGALPNEAA
                     IPITGPGTNPGTHGRWHSRVRGLPEFAGELPVACMVEEIATSGDGQVRALLTCAGNPV
                     LSTPDGRALDQALQGLAFMVAIDIYINETTRHADLILPPASPLTQDHYDSIFNAFAVR
                     RVARLNRALHGRGDQERADWEIVNGLGAAYAASAGKEWRALPRPRDLIAMGLARGDSG
                     LDLAALEAAPHGLDLGPLQPSLLARLQTASGCIECAPPVLLAELERFAARERADIADD
                     GLRLIGRREVRNNNSWMHNAPRLAKGKPRHQLLMHPDDMRARGLQDGGRVAVRSRIGE
                     IRTDVLGDAGLMPGVACLPHGFGHGRPGIQLGRA"
     gene            complement(21736..22443)
                     /locus_tag="PK399_01175"
     CDS             complement(21736..22443)
                     /locus_tag="PK399_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HPW11770.1"
                     /translation="MPFNYQVLKANNAGNDKDAVKKFMEAFPVVDPDFDPPNSGKYDN
                     DCADQLKIWQLNNGLVSDGVCGPNTWRAILGEDWDFNYRHAPSEVFQPTKRDCWRAAA
                     ATVLRVPMLSVPLGTAQYDNGGLNNAEGNLRTFASEHGLVYKKVSMSAGSLAFALGAY
                     KRFMVNVNDDYAGKVPGNNTHWMVLHRIRTDFWMTSGGTSVTFWDPMPVGQGQDKRTL
                     SFAKFAQFYICHMHRPN"
     gene            complement(22584..23414)
                     /locus_tag="PK399_01180"
     CDS             complement(22584..23414)
                     /locus_tag="PK399_01180"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPW11771.1"
                     /translation="MRSSDDMRPPAGPEAQSGDAPAHGEVTANIEMQILSRRISLQLT
                     VDSGPTRARALLPIAHGLTQIASDIACSDSEANGKPVSCKAGCGACCRQLIPITQTEA
                     HQLLAVVAAMPDTRRVEVERRFVDAVSRLQQADMLEPLRNAERWSRLDPVEFGDRYFR
                     LGIACPFLEHESCSIHEARPMTCRAFSVTSPAANCRNPGPDNIMPVQHPLPRPLDAFG
                     RAITGAAEGTPIPWVPLALALDYARSVPEQAPEYTGPQLLRAVFEAMTGVPVPEQTCA
                     "
     gene            complement(23621..24019)
                     /locus_tag="PK399_01185"
     CDS             complement(23621..24019)
                     /locus_tag="PK399_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPW11772.1"
                     /translation="MTTGKASPAKAAVGDKPVFAYIASLPQPQRGIAERIDRLAADHL
                     PGLQRSVKWGMAYYGVGQGWCFSCGAFAGHVKLMFVNGVTLDPVPPVTPVAMGKSTRG
                     VELASLDDIDGHPVVAWMRQITALPGVGKR"
     gene            23991..24731
                     /locus_tag="PK399_01190"
     CDS             23991..24731
                     /locus_tag="PK399_01190"
                     /inference="COORDINATES: protein motif:HMM:NF020909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HPW11773.1"
                     /translation="MAGLALPVVMESGSRFAGDDVGVAGLRVAIVCSGSGGMVHQATS
                     GCDRDRHRHDIGADCRARALGGYHADPTSMELPMSLSMHAASVPVFRQMLAALAGVLA
                     KAETHASERKIDPVALLQARLFPDMFPLARQVQIACDFAGSVSARLAGAEVPAYDGGD
                     ASFAELQRRIDATLAFIGGLDAAAFEGSEQRELVLRPGTPKERRIGGQAYLLAYGLPQ
                     FFFHVTTAYDLLRHNGVEIGKKDFMGAY"
     gene            24733..25404
                     /locus_tag="PK399_01195"
     CDS             24733..25404
                     /locus_tag="PK399_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HPW11774.1"
                     /translation="MEASDSPLLLTAAEARALGCLIEKEATTPDVYPLTIAAVLAAAN
                     QKTAREPVMALETGAVNHALRQLEAKGMARQVFSSRAERYEHRATQHFGLTSQQVALL
                     GLLLLRGPQTAGELLARSERLHAFADVEGVRHQLERMARREPALVVQLPRGGGQREDR
                     FAHLLCGPVDVSAIAAREPGGAGESSSPGLAARVAALEAEVAALREQLERLLQATTQE
                     TGTAT"
     gene            complement(25468..26607)
                     /locus_tag="PK399_01200"
     CDS             complement(25468..26607)
                     /locus_tag="PK399_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11775.1"
                     /translation="MDTTPHLARRLQPFHRIRRILAASLLLASAWSGSAAALDCGTNN
                     GYLCQGTQAQYAGGFAPGVGYGGFGGGTCTATRTPVVFLHGNGDTAISFDMPPGAVAG
                     YTTPARSQYDELKAAGYNDCELFGVTYLSADERANPQVNYHEPYTYDLIKTFIDKVKA
                     YTGKSQVDIVAHSLGSSMALAMIHYKGYQGSVRRFVNIGGGIRGLQTCVYTGYQSALA
                     PTCNAEAYVYPYNYYTFGLYPSSGVLYYGYNRWTGSGSGSMRSLPASWTAIRFYTLTA
                     GLRDQVHCFTSSNAASCPNGALFNARSNVIAQVNVGVGSSAYAYDWNWSDGIPFNAGG
                     GDNNGVGHFRSLANTGVIVRNMLTTTCSTGCANGYAGVNGPATNL"
     gene            26787..27710
                     /locus_tag="PK399_01205"
     CDS             26787..27710
                     /locus_tag="PK399_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPW11776.1"
                     /translation="MRMLLSRWCCIAVLLVGPIQAPAASASPECKATVTGDLRVHTLR
                     SAIFGNERKIRVLLPDGYADAANKDRRYPVLYLLDGQNVFDACLSDVSHHEWGADEAV
                     NRLVADNKIPPMIVVGVDHAGMQRAHEYLPYKDFVGNPDMEEPAGKQFPDFMAREVLP
                     LVDGQYRTLQGPANTGIGGSSYGGVASLYAMLAKPNVFGFGLVESPVLWIGLGQLVRD
                     TSPLVVMPRRVFIGLGGQESSDAAVNRKMLGLVRQVEANFHAAGYDTSNLRVVVDADA
                     QHTEAAWEQRLPSALAFLYGIQVTPRGDPPR"
     gene            27817..28167
                     /locus_tag="PK399_01210"
     CDS             27817..28167
                     /locus_tag="PK399_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11777.1"
                     /translation="MSQSFTAGTRRMQDGSFLPFVDVVDSDLGTEWQQPHPQGCVALE
                     AEAELRSLQFAQALREGGPVESPAQRYRQSSAAATQPEQPAGAVFSRFVAQLNRHQSL
                     LSGAERRTSMAEVA"
     gene            complement(28183..28851)
                     /locus_tag="PK399_01215"
     CDS             complement(28183..28851)
                     /locus_tag="PK399_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPW11778.1"
                     /translation="MRILLTGATGLVGQGVLHELLADPAVTHIGLLGRRPVEHADPRV
                     QSLVVERFDALDAVSDRLAPWDACFYCAGAPPVGTAEREYRHVTVDLTLHVARAFAER
                     NPQARFLYISGANANLRSRLMPLRVKGEAEAALQALPITTAMLRPGGVQPAHGERSPH
                     AWMRPMYALGRPMMGLGVRLLPGLMTSTAAIGRTLLALAAMPQPPRVVENLEINRIAG
                     SDQG"
     gene            28988..29764
                     /locus_tag="PK399_01220"
     CDS             28988..29764
                     /locus_tag="PK399_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW11779.1"
                     /translation="MRTPVEGLDVPAQVLVTGASSGIGLAMVEALLDNDGVAGVCAVS
                     RHALASPALQALAAGHPGRLHRIDADLATDAGIEAVAAGASQVTDVLHVVVNGAGLLH
                     GDAVHPEKSLATLDRAALEQVFALNAFAPILLARALLPLLDARSRCVVASLSARVGSI
                     GDNRLGGWYAYRAAKAAQNQLLRTLSIELRRSHPLATCVLLHPGTVDTPLSRPFQSRV
                     PAEALFTPARAARQLLDIIAGLQPRDSGRFIAWDGTDVPW"
     gene            complement(29775..30938)
                     /locus_tag="PK399_01225"
     CDS             complement(29775..30938)
                     /locus_tag="PK399_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HPW11780.1"
                     /translation="MNPLIHLIYCSAAPRPLGRDELATLLAGARAYNERHGITGMLLH
                     VDGSFFQVLEGAPEDVERLFARIGADPRHDHVTVIIREPVAVRAFGEWTMGHAEMSRH
                     ELDGIVGANDFFAGGRSLAEIGDGRAKKLLAAFRDGRWRTAPAGPIAAVEDAATLRYA
                     FAFQPIVHAPTRSIFSHEALIRGPDGENAASILQRSQPVEMHALRERQRIAALESAAR
                     LGLRTRININFQPSDIAHSPTAITSLLDAAARCGIPPESIVLEVLESEIIRDSGDFSA
                     RMNPYRASGLTFAIDDFGAGYAGLNLLADFQPDLLKIDMNLVRGIERNGPRQAILRGI
                     VRTCLDLGIDLIAEGVETVEEYAWFRGEGIELFQGFLLAKPMFDRLSTEFHLP"
     gene            complement(31024..32493)
                     /locus_tag="PK399_01230"
     CDS             complement(31024..32493)
                     /locus_tag="PK399_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HPW11781.1"
                     /translation="MKTRWIAAGFVVLLSASASVVRAQETHYYQADFPKEEFLQRRAT
                     VFAKIGDNAIALVQGAKGTADFNIFRQSNEFYYLSGIETPHAYLMLDGRSRTATLYLP
                     HRDAERERNEGKILSVEDEALVKSLTGVDAVRSIESLSLDLVGKGLVRPPALQLFTPL
                     SPAETGTDSRDELLNEQARVSSNPWDGLASREARFRALLQARVPQFEVRDLSPILDAM
                     RNIKSPREIAMIRRATRIAGMAIIEAMRSTEPGVFEYQLDAAAKYVFYLHGARGDGYA
                     SIIGGGSNAYLGHYFRKADRLKDGDMVLMDYAPDYRYYTSDVTRMWPVNGAYSAGQRA
                     LSDFILAYRDALLRHIRPGVTSDFVLDSAARDMARYLVGKRLDNPAHLQAVTDSLKFR
                     GHFQHPVGMAVHDVGLVRGVKLQPGMVFTIDPMIWIHGERLYVRMEDVVVVTEDGAEN
                     LSAFVPATTTEIERTMKDTGLVQFRPAIDVPAPDLHDSP"
     gene            complement(32574..32840)
                     /locus_tag="PK399_01235"
     CDS             complement(32574..32840)
                     /locus_tag="PK399_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11782.1"
                     /translation="MSDDGGLALLLLGPAGGAGLYWMLYRYYRNTDKSHAFERETVVD
                     AKPVTGAERDRKVDEIKGTRESRIQGDNLRDYRKRVQRIRGDSS"
     gene            complement(32837..34138)
                     /locus_tag="PK399_01240"
     CDS             complement(32837..34138)
                     /locus_tag="PK399_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009602102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HPW11783.1"
                     /translation="MSNTTPPPVPPTGPGSPRDVPPVPGSYPIDPATLPDPIRDELLA
                     PDPVAIDTAAEELKDGANRCPKCGATDIRQRPGTDLLICLYCRNEWHGQRVEEEFGFG
                     EGLDELTGTVIASGARDIDTDVSSLMTYKCTGCGAEVTVNTESTMTARCHWCRHVFGV
                     NEQVANGAVPDAVLPFHIKKDDAVARIRQFVDKRRLFALKEFKEQFTPENVVGVYLPY
                     MIVDGKASADVAGRGEIKTREYTQGSGDKKKTYYDADIYEVERHVDFTVDDLPLESST
                     ERGNLDTGVNTNNIINTILPFDTKNAVKWNASYLSGYTSEKRDTDVEQLRPRLEDQLL
                     SIARCQVEESVNRFDRGVRWEQERLDVHGTRWVAMYLPVWLYSYQQGGKGGMLHYIAV
                     NGRTGETMGSVPVQQWKLLLAAITVGTFLEGIALWMLGSST"
     gene            complement(34160..>34755)
                     /locus_tag="PK399_01245"
     CDS             complement(34160..>34755)
                     /locus_tag="PK399_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPW11784.1"
                     /translation="PASYLQPGKVFDFTDLDNAAAAQLFNEVVGSLAPAFSLYTNDPS
                     KGNRITKLQQDSIGFARSLSDAVEAGYQWKSDRGLVIAKTAIVSIEYDANTKELLKTV
                     QRADALSGARGNSNLQASVAQGIQSAGENGGAAGLMGVGMASGMMGIGNMQQPVAPPA
                     PAADDPIAKLKKAKEMLDLGLISQADYDALKAKALGL"
BASE COUNT         5377 a        11242 c        12274 g         5762 t
ORIGIN      
        1 ccgagacccc gataagggcg taacaatcct ctcttcggaa gcgcaagctc gcacccgagg
       61 agagtgcgtc gtcgtgcgac gcgacggcca gaccagtgct acagagtcgt ggagtactcc
      121 gtttgtagtc tagggaaaac gggatcaaca aaaggggacg gagggaatta agcacgctta
      181 attccctccg tccccttttg agagagcagg gcggccgcgg ggtttgaggt ctaccgggcg
      241 gttcagggtc ttttccagca tcgtctgaaa gcgaggattg cccagcgcct tctcctgcgt
      301 catgtgcgcg cggattcggg ccagttcttc gtcgtcggtt cctgacatga gccaggcgcg
      361 ataggcatgg gcgcgtgccg cgggcgtatc gccaagcgag aggtaaagcg catgcggggt
      421 caacagggcg tcctgcctga ggccaacaaa aacaggggtc agagtcgact ttttcagagg
      481 tcaggcaggt tgaggttggc cggctttaaa agtcgactct gacccctgtt ttctcttggt
      541 cgcccgatct tttgcgggcc gacgatgcgg ccgagctgcg cttcgatcgc ttgccggaag
      601 cgatccgagc cataggcatg ctgccggctc aggtgcaggc ggatcgcctc gaggtcctcc
      661 ggatggacgg tgtctatcac caaaaacagg ggtcagagtc gactttttca gaggtcaggc
      721 aggttgaggt tggccggctt taaaagtcga ctctgacccc tgttttctct tggtcgcccg
      781 atcttttgcg ggccgacgat gcggccgagc tgcgcttcga tcgcttgccg gaagcgatcc
      841 gagccatagg catgctgccg gctcaggtgc aggcgaatcg cctcgaggtc ctctggattg
      901 acggtgtcca tcaccatggc gcgataccgc tgttgtcgtt cgacagggcg ggcgccgagt
      961 gcgagatagt cgggatgcgg cgtgaccaat ggatcggctt cgccgaacgc caacgcgtgg
     1021 tggctggacc agcgatagtc gcgcgggtcg gcgaccatcg ctgcacgcaa cggattcagt
     1081 tcgatgtagc gatggcattg catcaggtag tcgccgtcgc ccaccaggct cgccttgtaa
     1141 cggccttccc agagcgtacc cgttcggcaa taggtcgcat tgatgtagcg gacgtaacgt
     1201 cgtccgaggg attgcatcgc cttcccgacg gcgcctggac gcgatggcgt cagcaagaga
     1261 tgcacatggt tcgtcatcaa gacgtaggcg tgaacggcgc aaccttctcg tcgcgccatc
     1321 tcgccgagtt cactgcggta gcacaggtaa tcggcatcat cgaagaagca aggctgccgg
     1381 tcgttgccgc gctggacgac atgctgcggg atgccgggga ggtcgatgcg gagcgggcgt
     1441 ggcatgttgt ggcctgtgcg cttggtggcc acaggttgat ccggcgctgg tgcagccgga
     1501 atcagtcagc gccgcgctca tgtgtcggaa aagtcgactc tgacccgggt ttcccgaccc
     1561 gggtttccgc gccaaggatt gatcctcgtc aatacgcgcg ttgttgctgg ctgggacagt
     1621 gcagcttccc gaggcaggag gcagtcatga aggcgaacgt gggcggtttt gacaagtgga
     1681 tccggatcgt ggccggcctg gctttgattg cgtgggcact cacgagtggg ccggtctggg
     1741 catggatcgg ggtggtgccg ttggctaccg ggttgttcaa tttctgcccg ttgtaccggc
     1801 tgattggctt caatagctgc tcacgctgag caaccagggc gtcagtcaga tgtgtccgtc
     1861 ttttccttcg gccgtccccg cgcgcggaca aggacctcgc gtttggcagg agctcctgac
     1921 aaaagggggc ggagggaacc aagggcgctt cattcccagc gttggctttt gaagagatgt
     1981 acctgacccc ttttcagtca ctggcatgac gaggcgggca gggtgagcga ttgcagggtg
     2041 cgcccctgcg cgtcctgcac gaatgcgacc aggccccagg gaccagccag cggttcgatc
     2101 gtcatggaac gcgtcagcgc cgtgcgcgcc atcggccaag gcccaagcaa cctgcgcacg
     2161 acgcggtcgt ggcggagcgt ggtgcccttg ttctcgccgc cccgcacggc cgtgacctgg
     2221 tcgtcctggt attgcgccac ccagacctgg gccggcgccg atggcgtgcc ggcgggtgcc
     2281 gcaccgagca tgagcaggga gccgccgttg gcctgcggca gcaggcgcag cgcgaggccc
     2341 gcgcttgccg gaccggactg gctgtccagc atctccctga atgaagcggg gttgcgccag
     2401 ggcgcattcg tcttgctgcc cacgatcact tgcggggtgt acaccgcacg cgcgccggtg
     2461 gcggcgacgc gcgcttcctg ccgccgcgag taggcaggcg acgcgaaccg gtcgggccag
     2521 ccgatgctgt cccagtagtc gacgtggaag gccagccagt tcaagcggtc cgcgccttgc
     2581 gtcagcgact gcgacagcca gcggtcggcg ggtgggcaac tgctgcagcc ttccgaggta
     2641 tagagctcga ccaacggttg cgtggccgat cccgtgcgcg cggcgcacga cccttcggca
     2701 aaggccggct gcgcgacaac cagcgcaagc cccagcaagc aatggcggac ggtcatggtg
     2761 gactccggaa gctgatgcgc ggcggcgtcg cgttcgagga gtctggcagc ctgggcagcg
     2821 tcgcgctgcg tccgcgcgcc cgcgaagcat gccccgacgt ccgaacgtac cgcccgagga
     2881 cggggccagg gttgattccc gcctcgctcc gtctggctag cccgacaatc cccgcaggtg
     2941 catccacagg aagagcggcg acatggccac cagcaccacg gcggctgcgg tatccagcca
     3001 cacgcgccgg gtgcgcagcg ccacgatgct gcaggtggcg gcgaccagcg cgaagccgga
     3061 gttcgacaac ccgtccagga ggctcgggct gcggcgcgcg atgaccagga tcgccagcac
     3121 cgagaaggcc aattgcagca tcttgtaacg tcggccttgg tgctcgtggt aagcgcgcag
     3181 gtccagcacg ctgccgtcgc ggggttcctc cggcatcacc aggtcgcaga acgcgtacag
     3241 cacgctgacg aagagcagtg ggacgagcgc ttcggatccg ctccagtcgc ttattccccg
     3301 catctgccag aacgccgccc agttgcccag cagcaacatc agcgccatcg cggtccagaa
     3361 gccgtggcgc caggaaaacc ggatgcgatc ggcatgccgg tacagcgcca cgccgccgct
     3421 cagcaggcgg gtcaacgcca gcgaggtaat caccgagatc atgccgaaca cgaattcgaa
     3481 gctggtcatg gtgcttgcac ccgccggaga cgcgccagcg tagcgggttg ccgacggcgt
     3541 ggcgttggcc gttcgcccgt gccgtgcccg ttgcctgcat cgaagcgtgc cccgtcgtgc
     3601 acgcaagggg gtgggtgcga tgcagcatgt ccgcaacgtg tcactggcgg tcgccttggc
     3661 cttgtgtacc ggcgcggcga tggccgcgga cgacgggcca tcggccggcg catgtttctc
     3721 ccaggccttc ctcgccgccg atgcggatgg cgtggcggcc tgttacgcgg aggacgcgat
     3781 catctggttc ccgggcggga cgatggccaa ggggcggccg gcgatccgcg atggcttcgc
     3841 ccactatttc tcggcgatga cggtcaagga cgtgcgcctg gccgagatgg gccacgtgat
     3901 gggcgagggc ggcagccgca cggcctgggg tacctacgag atgcgcatgg tcgacaaggc
     3961 gagcggcgcg gaggtggtga cgaccgggcg cttcaccgat gtgtccaggc gcatcgacgg
     4021 tcgctggctg tacatcgtcg accatccctc ggacgatccg ccgccggcga agtaaggcgt
     4081 tgagccgggg cctcgggagt ttcggcgctg accctggttt tcaggtcact tgcggacgtc
     4141 cagcagttcc acctcgaaca ccagcgaagc gcccggcggg atgacgtcgc gcgcgccgcc
     4201 ccggccgtag ccgagatctg cagggatgcg cagctcgcgc ttgccgccaa cgcgcatgcc
     4261 ggcaacgccg tcgtcccagc cgcggatgac gcggcccgcg cccagcagga acacgaacgg
     4321 ttggccacgg tcgcgggaac tgtcgaactt ccggccacgc ttgtcggccg cggcctggtc
     4381 gtacagccaa ccggtgtagt gcacgctgat ttcgtcgccg gcgactgctg tcgcgccctg
     4441 tcccgacacc acgtcgatgc gctgcagttc ggcgacgtcg ccgccggtgt agggcggtgg
     4501 ttggctgcag gcggtcagca gcaggatgga cagcgttgcg gtcaggcgac gtgcgcgagg
     4561 ggccatttca ggggtgcgga gggtggcggg acagcatacg ttgcagggca agcgggagaa
     4621 aaagaggacg gcggtcctgc ctgacatggg tcatggccga tgcccggagc gcgtgacatc
     4681 gtgatggcca agccgggcac gggccacggc atcggagaac caccatgctg cccctcagct
     4741 tcctgtcgat ccccgcactg gcattggctg cggcgctggt cgcggggcct gtggccgcgc
     4801 agtccggcac gcatgcgggc cacgacgcgc atgccggcca cgtcgcgccc gcaaccgcgc
     4861 cgcaacagcg atgggccacc gatgcggcgc tgcgcgcggg catgcacgat ctgcgggagg
     4921 cgaccgaagc cctgcatcac tacgagatgg gccacctgga cgatacccag cgcgacaacg
     4981 cggtgcagcg gatcgatgct gcgatcggcg acatggtcgc caactgcaag ctcaagccgg
     5041 atgccgacgc cgccctgcac gggctgctgg tgaagttcat cgccggtgcc aatgccgcgc
     5101 gcgcgggccg cttcacaaag gcggagctgg ggccgatgga gcaggcgctg gcgacctacc
     5161 cgcaacgctt cgacgacccg gagtggaacc gcacggacgg ccgcgactag cctcgggcga
     5221 ccgggcgaaa gggcggccac gtgcacgtgg ccatcgcgaa gggcatcgcc ttccacgcgc
     5281 catgttttcc cgcctcgtta gacgcctgac ctctttcccc tgcttccgcc gtgtcgacgg
     5341 gagacagccc cgcacaggtg ttgcaggaga acgtgatgtc gaaatgccat tccctgaccg
     5401 ccgcactggt cggcgcctgg ctggcaacag ccgctgcatc cgcgtccgcc gccgacccca
     5461 aggtcgaggc cagcgtcact atcgaaggca agacgatcgc attgccgcat gcgcgcgcct
     5521 tcagcaccgg catgcccttc gtgctgatct atttcgcgga gaagccgctg gacggcctga
     5581 agtacggcac cgctggcacc gacgccacct ggtccggtgg cgcctacggc acgatgcttc
     5641 gcttcgcccc ggcgcccgac ccgcgcgacg agggcaagcc cgtgcagcgc tatgtcatcc
     5701 ccgaggccgc cgccgaagaa gacgatcgcg tcgccctgcg ttcggcccgc atcggcaact
     5761 ggcaggccca atggttgagc caggaggggg tccggatcga gtccctggaa agcagcaacg
     5821 gcgtggttcg cggcacgttg tcgtggcgcg gcaaggcacc ggtcagcgcg tggtcggcgt
     5881 cgttcagcgt gccgctggaa gaaggcgtgc tgtagccagg cggcttcgcg aagaatctgc
     5941 agcggggcgg ggcaggtgca tggatctcgc aacagcgccc ggcgtgacaa gcgcgccacg
     6001 aaatgccctc caggagatca ttgttccgac agccgcgccc gcacgcgtgc cgtcgcttgt
     6061 gccaccacgc gctggacgtc ggcaagcgtg gtttccggca ggttgttgaa atccatcagc
     6121 cggtggttga gctcacgccc gcgcgtggct tcctccaccg cgaaccgcac ctcctgcagc
     6181 gcaacccggc ggtgatcgct gacccgcgcc gggtcatgcg agccgtaggt atcggccgag
     6241 gcgcgctcga gcgcgcagta gaggctccgc ttgttcgcgg cttcatcgtc ggcgcagcgg
     6301 cggtcgtcat tgcggttcca ggcggagtcg ctggccagca attcggccgc gcgctccagc
     6361 gttcgcaggt cgttgtcggt gacaggcagg tcgcgggctt cgtactgggc tggatcgccg
     6421 aacttgtcgc gcggtgacgc cgactgcgtg gccggcgtct gcggtccgtt cgatgcgcag
     6481 gcgacgaggg aatacgagag aagcaagggc agggttgcga gagcggtccg ggtggccatg
     6541 gcgcgtcctg cagtcgggtg tgttcgtgat gctgccccaa aaagggcgag agcggggctc
     6601 gggtgcattg gtcgcttgcc cttcctgcct gcgcggagcc ggttcccatg ggcgagtccc
     6661 tgaatccagc acctgacgcg gggcagaagg tgccagcggc tcctgcgtta cggaccgatg
     6721 acggcggcgc gcgcccaacg tcactgctgg ggacgcgcct gcgctggttc ctggccgagt
     6781 tcctggtggt ggtcgcgggt gtgttggtgg cgatgggctt gagcggcctg gtcgacaaac
     6841 ggcgtgacct gcagcgcgag caggtctacc tgcagcaact cagtgcggac ctgggcacga
     6901 gcgaaaggaa ctgacggagg cggtggcctt catgcaggcg cgcgccgagg cggccgcgcg
     6961 cgtgctgcac cggttctggc gcaaggaccc ggtgctggac gattcgtttt tcctggatct
     7021 gtcgcagccg cgcggcaccc ggcgcttccg gccggtgttg gggacggccc aggcgctggg
     7081 atcgtccggc gagctcgggc tgatccgctc cgatgcgctg cgcgcagacc tgctggcgta
     7141 tgtcgagcgc atgaagaccc gtctcgacga aatcgatcgc tacgacgaga cctactatcg
     7201 gcccggcgtg gccatgctca acctggcgcc gctctggcgc acgccggaaa attccgcagt
     7261 gctgcagtac tcggacgaca aactccgctc gcgacccaac ccgttcgatc gcgtcccgtt
     7321 tccaaacgag ccggcggacc agcttgccag ccgtgaggtg tatgtcggtt acagcaacct
     7381 gctgaccgcg caccgcaacc acgccgtgca atacgaggaa atgctgcagg agacgcgcgc
     7441 gctgcggaaa aacgtggaag cccgcatccg gcgttgaccg cgggtggatg acgcccgctt
     7501 tgctccatgt ctcgcccaac cacggcgacg cctggacgca gatcggccgg ccgccctacg
     7561 tcatctgcga ggtgcagatg gacagcgtgg accgcggctg gaccagtcgc tggaacatcc
     7621 atcttgcggg ccgcctcgcc cggggcctgc ccggaatgca ggtggccgga gacgtgccgg
     7681 atcccggtac tacagcaatg cgcaaaggct cgcctggtcc atggccctgg cgaacagcca
     7741 cccctgcatc cgccgcaccc cgatggcctg caaggccaac tgctggtcgg cgcgctccac
     7801 gccttccgcc accacttcga taccgagtga ttccgcgagg ccaaacaccg ccttgacgat
     7861 ggcgacgtcc gccgtggtgc cagggaggcc gtccacgaag ctgcgatcga tcttgatcac
     7921 gtcgaccggc agccgcctga ggtagaccag cgagccgtag ccggtaccga aatcatccag
     7981 ggccacctgc acgccgagtg ccttcagtcg ctgaagtacc ccgagcgcgt ggtcgaggtt
     8041 gcgcatcagg gtggtttcgg tgatctccag gcaaagccgt tccggcgcca ggccggacgc
     8101 cgcgagcgca tcgcccacgt cggcgaccag cgccgcctcc tcgaactggc gcgccgagac
     8161 attgacccgc accgtcggcg cgacctggtt cggggcgccc gcccattgcg cggcatcggc
     8221 gcaggcgcga tgcaggatcc agcgccccaa cgacaggatc tggccggagg attcgagcac
     8281 ttcgatgaac tccgcggcgg ccagcagccg cccctcccgt cgccagcgca gcagggcctc
     8341 cgcagcgacc caggcctgcg tcgtggcgtc gtattccggc tggtagtgca attcgaattc
     8401 gccattggca ggcgcatcgc gcagcgcgcg ctccaggcgc aggcgcttga gcagcgcgtc
     8461 gtggtgctcg aggtcgaaga cctggtagcc gaacttgtcg tgttcgcggg cccgctccgc
     8521 cgcggactcc gcctggcgca tcagcacgcg tgcgccgccg tcggcggccg cgtccgcgta
     8581 cgcgatgccg acggtggcgc ccggcagcag atccttgaga tcccattgca ggccgcggac
     8641 cgcggcaata cagcgttcgg ccagccgcac ggcgtttcgt tgcgcagtgc tgccctccag
     8701 cacgaaggcg aagccgtcgg cacgcacgcg tgccaggtgc gtctggttgc tgctgagctg
     8761 acgcaagcgc agggccacct ggcccatcac gtcgtccgcc gtcggggcat cgtcaggcaa
     8821 ggccacgccg cccgacgcat cgatgcgcac gtggacgacg gccagcacgt cgccaggcgc
     8881 acgtggtgct gccaggcgct ggcgaagcag ctccaccagg tagtcgcggt tgggcagtcc
     8941 ggtggtcgcg tcgtggagca acaagtgctg cacttccttc tccgcccgac gccggcgcac
     9001 gatctcgaac gccatcgcca cgaaatcacc catgctgccg gcgaaggtga tttcgtcgtg
     9061 cgaccagagg cggggacccg ccgtgcattc gtggcagatg acgccgagca gctcgccctc
     9121 cacgcggacc ggcgcgtcga gcaatgcctg gatgccatgg cgttcgaggt agcttcgcaa
     9181 ctcgctgagc gaactcgccg tgctgggatg gaggcgtacg tcggcagcgt cgagggcgcg
     9241 cacgtcctcg aacccggtca cgtagtcgtc gccttcgagg gacagcgatt cgagcgtgtc
     9301 gtcgctggca tgggcccgct cacgggcgga aaaagcatgg atgcagcgca ggcgctgggc
     9361 gtcgcgctcg tagtgccaga cattgacgcg gtcgacgcgc agcgcatcgg ccgcgtgttc
     9421 gcagatcacg gccagtgcgc tgtcgaagct gcagtcggga tgccagacgg cgcgggtcag
     9481 cgccaccagc gcccgggtga agcgctggcc atccgatggc gtcgcatcgc ggccaggctc
     9541 ggcttcgctt ggcacagggg gtgtcggcat cggcgcggtc cgtggcttgg gacgtgtgat
     9601 cagactagca gcaggaatct aggggcaggg acaacagggg gcggaggaaa acagcatcgg
     9661 tgaccctctg atccgccaga tggcgaaatg gcttgcctca gtcgtgcacg gtgccgcgtc
     9721 ggtcggcgac ttgctgcgcg tgcgggtcga attgatccgc catcatgacc cggttgcggc
     9781 cgccgttctt ggcccggtac agggcggcat ccgcgcgggt gagccagaag gcgccccgct
     9841 cgccgggcgc gagggtggcg gcgccgatcg acaccgacac cggctcgccg tccggtcgcc
     9901 tgagggtctg gaaaatctcc tcgcgcaaac catccagcgc gcgctggacg ccgtgcgcgg
     9961 cggtgtccgg catcagcagg atgaactcct cgccgccaaa gcggtagagc cggtcctgtt
    10021 ggcgctggtg gatgcgcaag gtgcgcacca tctcgaccag caactcgtcg ccgacctcgt
    10081 ggccgaacgt gtcgttgacc gacttgaaat gatccaggtc gatgatcgcc agggtgcacg
    10141 gagactggct gagctgatgg gcctgcaacg tggtggccag gtcgatgtcg aacgcgcgcc
    10201 ggttcatcgc ctgcgtcagc gggtcatgga tcgccatgtg ttccagcgcc tgtcgctggc
    10261 gggtcgattg cagcgagaag atgaaggcgc acaacgacac cagcaatccg ctgctgagga
    10321 acaccacccg ctcgatatgg ttcgcgaaca cttcgggatg gctgccgagg atgccgagca
    10381 cgatcacgtt ggcgatcatc gccatgcgtc ggctggtcat caggaaattg gtggtcagca
    10441 ccgcgtagga ccacagcagg ccgtggcgcg cgaacagcac cgacaccacc aggcaaccgg
    10501 tcgtggtcac gatcgccatc aggaccccgg cgcgctcggt ctgtccgggc agccacgcga
    10561 caaggccgac cgcggacacc gcgatcacga tcaccgcatc gatgatcccc acgccgatct
    10621 ggccctgcgc aaagcgatag atcgagaagg gcagcacgat cagcgcgatc agtgcggcga
    10681 agaagctgat caggccgagt tgcgggtcgt cggtcgcacg ctcgacggcg gcctgccagc
    10741 cccccggggg cgcctcccca cgcgcatgcc gcccggcgcc ctgcggggat gacctctgcg
    10801 gtgaggaccc gttcatcgcc cgcaagcata aacctcggcc ggctggcgtg cgcggcaagg
    10861 agcgggcagg ggacaccccc gtctggaacg ctcgacccgg tacctgtcga gtgccgatgt
    10921 gcccgggccc cgcttgccgg tcagccgatc tggcgtgcca ccgcgtcgaa ggcggctgcg
    10981 atgcgcgtgg cgacggcctg cgcggattgg ccgttgtgct tcaggttggc cagcgccacg
    11041 tcggcaatcc ccatgagccc ggaaatgttc cgctggtatc ccgatgcaac gatgccgatg
    11101 accggcaggc ccaccagtgg ctgcagcgca gcggaaaggc ttgaagcgcc gcgcgcgagc
    11161 ccatcgcgaa tccggctggt gacatacgca gccggatcct gtccgagcgg aacggtctgg
    11221 tgggagatcg cgcgcagggg cgagaccacc atgttgacgg cggcattcac cgtggctgcg
    11281 acgcggtcat agacgaattg ggggacgtag tcggaatcga tccacgagat caggcgcgaa
    11341 ccgatcttct ggtagccggc gatgacggcc tcgatcacca cattgaagac ggacagatcg
    11401 acgaatcccg gcgggcctgc tgccggtggc gcgccacctg gtggtgggat tgcgcctgga
    11461 ggtggcggtg cgcccggtgg cgcgtagcgt gccggtgcgg ggccggacga cccgcccagc
    11521 gacaatccga gtgcggccgc ggtcttggga cccaccacgc catcggccac caaccgcttc
    11581 atctgttgga acgccttgac cgcctgggcg gtcttcggac cgaacgatcc atccgcgacc
    11641 aggcgcggcg acggtaccag catggtgttg agctttgcct gcaacgctga aaccgttggg
    11701 ccgcggctgc ccaactgcag tgaattcgtc atggagggac ctgtgcaagg ggaggaaccc
    11761 ggtaggcgtc cgggccgcgt gggttgggag ccctgctgcg ggccttgcgc cgacacgaat
    11821 ggccgcgatg ctggcatgca cgcatgggcc gttccatcgg ccagacagcc aatgtcgatt
    11881 ggcctgccat cgacgtgcca gggcagcgcg cgtgcctcga tcgggaatcc attgagcgcg
    11941 cattgccgtg gccgccctcg cttgcaggcg ggtcggtcag gtcgttgtcg cgctcatgcg
    12001 cgactgaaga tcgctacggg gcaggcaggc cgacatcgtc ggagtgcgcc gcgcatgcgt
    12061 cttggcattg accgcaaccc cgatgcgggg agctacgatg cggcgtctgg atggggggac
    12121 acgcgggtgc cggatcgcgg tcgtgggcag gcaagtcaca tgcgtcgatg gctgctggcg
    12181 atgctggccg gcatcgcatt gctggtgctg gccgagggcg cgttcgccca ggtcaagcgc
    12241 aaccgcttcc agaacctgga caccggcaag accgggggcg tgcgtgccct ggcggaggat
    12301 gccgatggcc tggtctgggt cggcggcgac gcgggactgg cgcggttcga tgggcgccag
    12361 atggccgcga tcgcgcccga caggctcacc ggtccggtca atgcattggc attgcaggga
    12421 cgtgtccgcc tgtggatcgg cggcgagcag ggactgctgc gttgggacct ggccggggag
    12481 cggctggccg aagcgccctg caagggcatg gtcgatggca tcggccaggt gcaggtcacc
    12541 ggcgacggcg tgctgggcct gtcccgatcg gggctgtatc gggtcgatgc cgacaccctg
    12601 gcctgcagcc cggtgcggat cgcgggattg cccgagggcc aaccggtcga gcggttctgg
    12661 caggacgaca ggcagttgtg gctggcgatc cgtgatcgtg gcctgtggcg ctgtgccttg
    12721 ccctgcgccg cggcgcgtcc gtgggcgagt gcgctggcgg aggagcgcat ccgctggatt
    12781 tcagccgcgc cgcgagacgg cctgtatgtc gggacccatc gtcgcggcct cttccggctc
    12841 gatcgcgatg gcgccgtggt cgatcactgg tggcgcggcg acgatgcgcc ggcggatcgt
    12901 gcgttcacca ccaacggcgt gatggcgctg caggcgatgc cggacggccg cctgcatgcg
    12961 ggcttgtggg ccggcggcct gatggaggtc gccgcggatg gcgcggtggt ctcgcgctcg
    13021 cgaccgttgc cgcatgaggg gaccagtctt gccgggctca acgtccgcgc gctgctgctg
    13081 gcgcgcaacg gtacgttgtg gatcggccat gaaaacggct tgagcctgct ggatccgctg
    13141 cgcaaccgat cggcatggat cggtccggtc gcggaaggcc agccgggggt ggatgccagc
    13201 ggggtgagcg cgatcctccg cgatggcgac atgctgttcg tcggcaccgg gcgcgggggc
    13261 gtcaatcgcg tcgacctggg caccggcgcg gtcacggcgg tgcaacacga tccgcggcga
    13321 cgcgattcgc tgcccgacga tgcgatctgg gacatggccg ataccggcga tggccgcctg
    13381 atgctggcca cctcgggcgg cctggcgcgc ctgtcgaagg acaacctgcg ggcggagacg
    13441 ctggcgacat cgccggtcct gccgagcgat gacgtggtca ccgtggcccg ggcgcgtgac
    13501 ggtggctact ggctggcgac atgggcgggc ggggtggttc gggtggacgc agccggcggc
    13561 gtggccagga cctggcgtgc ggccgatggc ctggacacgg agaccatggt ggcggtctac
    13621 gaggatgcac gcggccgcgt gctggcgagc aatgccgacg ggctgttcct gctcggccgc
    13681 gatggccgct tccgcacggt cgaggtgcag ggcacgcatc gccgcggcaa gccggcggac
    13741 atgctggcct tccatgacgc tgccgatgga acgctgtgga tgggcgcggc gtcgggcgga
    13801 ctggcccgct ggccggccgc ggccgagcga ccggcctggg tcgatgaacc gaccttcggc
    13861 gacatcggca tcaatgccat ccaggcgcgc gccggcggcg gcctctggct tggcagcaac
    13921 accggcttgc tggcggtgga tgcgcagggg cgggtgttgg cccggatggg cgtgggcacc
    13981 ggactggaat ccgaggtcgt gaccgcattg ctgggggagg agggcggcga tctgtgggtc
    14041 ggcggcgcaa gcggcgtgca acgcatcgag ccgggcatgg ccagcgtcgc acgccaggat
    14101 gccgcgccgg tgatcagcgg ggtgcgcctg ttcaatcggc cgttgcaccc ggaccccgag
    14161 ggcgcgctga ccgcatcgcc cacccatggc ggcgcgttgc gcctgcggta cgaccaggac
    14221 atgttgaccc tggattttgc cctgcccggg ctgccgcaac cggtgggact gcgctaccgc
    14281 tatcgcctgc agggcttcgc cggcgactgg gtcgaggtgg ccgcggacga accgcgcgcc
    14341 gtgtacaccc gcctgccgcc cggcgattac cggttccagg tacaggcggg cgatgccggg
    14401 cgctggtcgg atcgcatcgc cgagttgccg gtctcggtga gtccgccctg gtggatgacc
    14461 tggtgggcgc gggcgctgtt ggtgctcgcg atcatggcgg tgctggcgtc ctggtaccgc
    14521 tggcgcaccg tgcaattgcg ccggcagtcg caggtgctgg aacagacggt gctcgaacgc
    14581 accgcggcgc tgcgcgaagc caaccaggcc ttgcaggcat ccgcgcgcac cgatgccctg
    14641 accgggctgg cgaatcgccg cggtttccag gatgcggtgg gcccgcgctg gacggcattg
    14701 accggcaatg ccgccttgct gctcgccgac atcgatcact tcaaggcgat caacgatgcc
    14761 ttcggccacg acatcggcga tgcggtgctg gtggatgtcg gcaagttgct cgacgcggtc
    14821 gcccgcgatg gcgacctggt cgcgcgttgg ggtggcgaag aattcctgtg cctgctggtc
    14881 ggcaccgatg cgcgcgcgcg cgccgaggag atgcgggcgg cagtctccgc cgccggtccg
    14941 cgcgccgcgc tgggcgcgac ccgggtgagc atcaccgcag gctatgccgc catccgagca
    15001 ggcgaaagct tccaggaggc cgtgcgccgc gccgatgcat tgctgtacgt gggcaagcgc
    15061 ggtggccgta accgggtggt gttcgaggat tgatccgcct ggaacaccac cggttcgcat
    15121 gcgcgcctga gccagtatcg gccgtcctgc gaacgtcgat caggatggaa ggctttttcc
    15181 ggcgtgcctc gggcgcgtca gcaatgaagg tagattcgcg cacatgtaca gcgagctgaa
    15241 gcccggtccg atctgttcga ccatcgcgac cttgtcgcgc cgcatccagg agcgttttcc
    15301 cggctcgggc ctgggcaagg tcgcggacga actcctgcgc gtggccgggg acaaccagcg
    15361 cgtggccgag cagttgcgag gcccgatctg gaaggtgcgc gtcttcacgg ccctggccgt
    15421 ggtgatgctg ggcgcgctgg cgtcatgggc cgtgctcgaa ctcgcacgga gggccgcggg
    15481 cggcattggt ggcgttgccg atctgttgca ggcggcggat gccgggacca atcaactgat
    15541 cctgctttcg ctcgcggtgc tgttcctggt ctcgctggaa acccgcatca agcgggctgc
    15601 cgcactgcgg atgctccacc agttgcgcag catcgcccac gtggtggaca tgcaccaact
    15661 gacgaaggac cccgagcatg cgctgcgctc cgtggcatcg acggcatcct cgccggtgcg
    15721 cacgctcacg cgcccgcagc tcgcccgtta cctcgactac tgctcggagc tgctggcctt
    15781 gaccagcaag ctcgccgccc tgcatgccca gcacctgcac gatccggtgg tgctcgatgc
    15841 ggtcaacgac atcgagacct tgaccgccga cctgtcgcgg aagatctggc agaagatcac
    15901 catcctcgac atgggggagg agcgtgcggt acccgccagc ggcggggccg ggatggggcc
    15961 cgtgtccgcg catgatgcaa acggcgccgg gcagggacgc tgaggcgact cgggaaagga
    16021 gaagagggcc gggagggaac actcccgctt ggaaggcgcc gatcacccag gcgaccgtcg
    16081 cgagggcttc acggtgccct tttcccggcc ttcacggaag gatcgcttac gctggcatgt
    16141 caggcgcatt ggcgccgcat gttccgggat tgggatggga ccgctcgatt tcatcaacgc
    16201 cggtggggag atgggcgcga tgattcgcgc ccacgactgg gcgtcgtccc cgatcgggcc
    16261 gcccgaacaa tggccgcagg ccctgaagac cgcgctgcgc atggcgctga ccacccgcca
    16321 cccgatcttc atcttctggg ggccggaaca catctgcttc tacaacgacg ggtatcggcc
    16381 gtcgctgggc ccggaaaagc atcctgcctt gctgggcgcc cgtggcagcg aactcttcag
    16441 cgagctgtgg gacatcatcg gcccgcagat cgacatggtg atgcggggcg agggcgcgac
    16501 ctggcacgag aaccacctgg tcccgagcta tcgccacggt gtgctcgacg aggtctattg
    16561 gacctacagc tacggcccga tcgacgacga gaccgcgccg ggcggcgtgg gcggcgtgtt
    16621 ggtggtctgc accgagacga cccagcagat caaggtcgcg aatgcgctgc gcgagagcga
    16681 gcgcgcgctg aagcaactca acgacgagtt ggagcagcgg gtgtcgatcg aggtcgccga
    16741 acgcctgcgc gagcaggcac gcctggcgga ggcgcaggaa gcgctgcgcc agtcgcagaa
    16801 gatcgagacc ctcggccaac tcacgggtgg cgtggcccac gacttcaaca acctgctgac
    16861 cccggtgatg ggcgcgctgg atttcgcgcg gttggagctg cagggcgacg aggtggcgca
    16921 ggaagtgatc gcggtgggcc tgcaggccgc ggagcgcgcg cgtacgctgg tgcaacgcct
    16981 gttggcgttc ggcaggcgcc agcacctgca gccgcaggac atcgaagcgg agagcctgat
    17041 ggattcggcg gtggagctgg cccagcgctc gctgcgtccc ggtattcgcc tggtgctgga
    17101 catgccggac acgttgcctg cggtccatgc ggacccggac caggtggtcc tggcggtgtt
    17161 gaacctggtg gtgaatgcgc gcgatgccat cgaaggcgtc ggcaacatca ccctgcgcgt
    17221 gcgcgaagac aacgatcctg gccaggcggg cctgttgccg ggacggtatt tgcgcatcga
    17281 tgtctgcgac gacggctgcg gcatggacga agcggtggtc gcgcgtgccg cggaaccgtt
    17341 tttcaccacc aagccgaccg gccagggcac cgggcttggc ctggcctcgg tgcaggggct
    17401 ggcggtgcaa tcgggtgggg gattcaagct ggcaagcacg ccgggtgagg gcaccaccgt
    17461 aagcctgtgg atgccggcgt cgaccaagcc cggcatcgac agccaacggc aaggcgcgca
    17521 gcgcagtcgc acgcaggtgg cgaagtgcca ctgcatcctg ctggtcgatg acgatgcgtt
    17581 ggtgcgcgcg agcggcacct ccagcctgcg ccgcgccgga tacgacgtcg tgcaggcggc
    17641 ggatggcgag gaagcactgg cgttgctgga cgcggccggc cccttcgacc tgctggtgac
    17701 cgactacgcc atgccggggc tgagcggcct ggagctcatc gacgcggtac gcctgcgcaa
    17761 gccgcagatg ccgatcctga tggtgaccgg ctacgcggac accggccagg aggctgccga
    17821 cgtgccgcgc ctgcagaagc cgttccttcc ggcggaactg gtggatcgcg tgcaggcctt
    17881 gatcgccgag gcctgatcgg caggcaagcg cgccggtgga tccatcccga tccgatggcg
    17941 gccttggcca ggcccagggc aacgcgtcca ccttgatcgg ccgcgcatcg agcgcggcat
    18001 tgccgcagcc gccttcgatc gcaagcgggt cggtgaggtc gttctcgctt tcgcccgcgg
    18061 gtgacgaact tcatgcgagc ggtgtgacgc gcccctcgtt cgcgtcccgg tagaagccca
    18121 ggagtggcgc gcgttgcctc aaggaaccaa tgcggacagg tgccgcggtt tccgattcct
    18181 tctcagggtt tgcccaccac gcggatcggt cgggtggcga tggtttcgcg atcacggaag
    18241 acgtagcgca gctcgtagtt gccgggctgc tccggcatgg tcagctccac ctgcctggaa
    18301 tcgctgccga ggtaggtgta ggagatgtag ctgtcgctgc cctgtacggc gacctggatg
    18361 ttgtcgagcg acgcattcgg cccagtccag tggatgacga cggcgctgcc ggcctgggca
    18421 acgtcaggtg ccttgagccc ggcctgcgcc ggcgacgcct ggatcgggcg cgtggcgatg
    18481 acggtctggt cgcgatggcg atactgcagg gtataggcac cggactcgcc gggcatggtc
    18541 agcgtcagcg ggttgccgtc cttgacgtag gcgtaatcgt gggtggtcgg cttgccgtcc
    18601 acctgctcga cgatggcgac ggtatccagg ttcccggcgg ggccggtcca cttgatcgcg
    18661 acccgcgagc ccatcggtgc actggcaggt gcgctgaggg tggcggcggg cttggctggc
    18721 gctggctcgc tcaccgtctg gcgcaaggcc gccgagaggg cggccccatc cgaggcttgc
    18781 aggtagcgac caccggtatt cgtggccagg caggccacct tcgcgccttc gtccctggtc
    18841 aagccgaagc cgatgacgtg ggcagtgaag ttcaccccgc tcttctcgag ttcgttggcc
    18901 accgcgcagg gatcgaggtt gcaggtttcg agcccatccg tgaccagcac cacggtggcg
    18961 gcttgctcgg tatggcgcag ggcctgggcc gcctggcgca ccgcttcgga gagcggggtc
    19021 atgcccagga agcgcatgtc attgaccgcg ttgagcacct tgccggacgt gccgggcgca
    19081 ggcggcacga tcagttcgat gtccttgcag tcgcccttgc ggcggtggcc gtaggcgatc
    19141 aggccaaggg tctggttggc gggcagcttg cccaccacat cggcgacggt ttcgcgcgcg
    19201 atctcgagct tcggacgccc gtcgatctgg ccccacatcg atcccgatcc gtccatgacg
    19261 atgatcgtcc gcgcttgctg cggctcgctg gcggcgaccg aggccgtggc aagcaggagg
    19321 gcgctggcga gcagcgcggg gcggagtggc gacggcatgg acatggcgat gggtcctgtg
    19381 gaggaggggt ggtcgggtca cttacgcagc tgcgaaagcg atccggacat gtccctggcg
    19441 tgcaggacgc aatgacagca cgcaaccagg cggagaacgc cgccgggaaa tgcattcccg
    19501 cgcgtacctg gcccggagga cagcgtccgg gcgggcggac gctgcgtgat cccatcgcac
    19561 ctgtcatcgg gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcgcgcc
    19681 ccaactggat gccgggacgg ccatggccga agccgtgcgg caggcaggcc acgccgggca
    19741 tcaggcccgc atcgcccagc acgtcggtgc ggatctcgcc gatgcgcgaa cgcaccgcga
    19801 cgcggccgcc atcctgcaaa ccgcgcgcgc gcatgtcgtc ggggtgcatc agcaactggt
    19861 gacgcggttt gcccttggcc aggcgtgggg cgttgtgcat ccacgaattg ttgttgcgca
    19921 cctcgcggcg cccgatcagg cgcaggccgt cgtccgcgat gtcggcgcgc tcgcgtgcgg
    19981 cgaagcgctc cagttcggcc agcagcaccg gcggcgcgca ttcgatgcag ccgcttgcgg
    20041 tctgcaggcg cgcgagcagg gaaggctgca acgggccgag gtccaggcca tgcggcgcgg
    20101 cctccagcgc ggcaaggtcc agcccgctgt cgccacgcgc caggcccatc gcgatcaggt
    20161 cgcgcggccg cggcaaggcg cgccactcct tgcccgcact ggccgcgtag gcggcaccca
    20221 ggccgttgac gatttcccaa tcggcgcgtt cttgatcgcc acgcccatgc agggcgcgat
    20281 tgaggcgcgc caccctgcgt accgcgaagg cattgaagat cgagtcgtaa tggtcctggg
    20341 tcagcggcga ggccggcggc aggatcaggt ccgcatggcg ggtggtctcg ttgatgtaga
    20401 tgtcgatcgc gaccatgaac gccaggccct gcaacgcctg gtccagtgcg cgtccgtccg
    20461 gcgtggacag caccggattg ccggcgcagg tgagcagggc gcggacctgg ccgtcgccgg
    20521 aggtggcgat ctcctcgacc atgcaggcca ccggcaactc gccggcgaac tccggcagcc
    20581 cgcgcacgcg gctgtgccaa cgtccgtgcg tgccggggtt ggtgcccggc ccggtgatcg
    20641 ggatcgccgc ctcgttcggc agcgcgccgc cgacgcggtc caggttgccg ctcaccaggt
    20701 tcaccaattg caacaggacc tggcacagcg tgccccaggg ctgcactgac acgcccatgc
    20761 ggccgtaggc cacggcatgc ggggccgcga ggaactcgcg ggcgacctgc tcgacgacat
    20821 gcatcgcgat gccggtctct gcctccagcg acgcggtgtc gatggcattg atcgcggcca
    20881 gtgcgatgtc gagtccggcc aggcggcctg cgtacgcgtc cagccgcggc gggctgacct
    20941 gctgcatggc ttgcagcaac gccacaagga acaacgcatc gccacccggg cggatcggga
    21001 tgtgctggct ggcgatggcc gccgtttccg tgcggcgagg atcgaccacg accagcttgc
    21061 cgcgcgcggt cagcgccttg agccggcgcg gaatcccggg cgcagtcatc aggctgccgt
    21121 tcgacgccat cgggttggcg ccgagcatca ggaaatagtc ggtgtggtcg atgtcgggga
    21181 tcggcagcag gaactggtgg ccatacatct gggcgcaggc gaactggtgc ggccactggt
    21241 ccaccgacga cgcgctgaag atgttgggcg tgcgcagcat ccgcagcagc gagggcaggt
    21301 acgcgatatg cccgaagtgg tggacattcg gattgcccag gtacgccgcg accgacgcgg
    21361 ggccatgcgc ttgctggatc gccgccagcc gcgaccccgc cagcgcgaac gcctcgtccc
    21421 agccgatggc gcgccaggcc gaaccctcgc gcagcatcgg cacgcgcagg cggtccgggt
    21481 ccgtctccag gtcgaggatc gcattcccct tcgggcagat gtgcccctgg ctgaaggggt
    21541 cggcggcatc gccatggatc gcggccagcg cgccgtcctc gaaccgcaac tccagcccgc
    21601 agatcgcctc gcacaggtcg cacgcgcgat aggcgttggt acgcatggag aagctcccgg
    21661 gcaaggttgg cgaacgccga tcctaatgca gccgcgccgg ttgcgcggcg gcgcgaccgc
    21721 gtgccgacgt cgacctcagt tcggcctgtg catgtggcag atgtagaact gcgcgaactt
    21781 cgcgaacgac agggtgcgct tgtcctggcc ctgcccgaca ggcatcggat cccagaaggt
    21841 gacgctggtc ccgccggatg tcatccagaa gtcggtgcgg atgcggtgca ggaccatcca
    21901 gtgggtgttg ttgccgggta ccttgccggc gtagtcgtcg ttcacgttca ccatgaagcg
    21961 cttgtacgcc cccagcgcaa aggccaggct gcccgcgctc atgctgactt tcttgtagac
    22021 caggccatgt tcgctggcga aggtcctcag gttgccctcg gcattgttca gtccgccgtt
    22081 gtcgtactgc gctgtcccca gtggaacact cagcatcggg acccgtagca cggtggcggc
    22141 agccgcgcgc cagcaatccc gcttggtcgg ctggaagacc tcgctcgggg cgtgccggta
    22201 gttgaagtcc cagtcttccc ccaggatcgc ccgccatgta ttgggcccgc acacgccgtc
    22261 cgataccagg ccgttgttga gctgccagat cttgagctga tcggcgcaat cgttgtcgta
    22321 cttgccactg ttgggcggat cgaagtcggg gtcgaccacg ggaaacgctt ccatgaattt
    22381 cttgaccgcg tccttgtcgt tgcctgcgtt gttggccttc aggacctggt agttgaatgg
    22441 catggatctt cccgccgcgt tgatgccgat ccccgatcca acggggaacg cggcccccgg
    22501 cggtcaggcc cacgcgtgga acgacgacgc accgcggagc acgcaagccg aggcggctgt
    22561 tcgcgttccg gggccgcctt ccctcaggcg caggtttgct ccggaacagg gactccggtc
    22621 atcgcttcga acaccgcgcg cagcaattgc gggccggtgt attcgggagc ctgttccggg
    22681 acgctgcgtg cgtagtccaa cgccagcgcc agcggcaccc acgggatggg cgtgccttcc
    22741 gctgcgccgg tgatcgcacg accgaaggcg tccaagggac gcggaagggg atgctgcacc
    22801 ggcatgatgt tgtccggtcc cgggttcctg caatttgcag ccggtgaggt cacggaaaat
    22861 gcgcggcacg tcatcggcct tgcctcgtgg atgctgcacg actcgtgttc caggaacggg
    22921 caggcgatgc ccaggcggaa gtacctgtcg ccgaattcca ccggatcgag gcggctccag
    22981 cgttctgcat tccggagcgg ctccagcatg tcggcctgtt gcagtcggga gacggcatcg
    23041 acgaaccgtc gctcgacttc aacccgtcgc gtatccggca tggcggcgac gaccgcgaga
    23101 agttgatggg cttcggtttg ggtgatgggg atgagttgcc ggcaacacgc gccgcacccc
    23161 gccttgcagc tcaccggctt gccgttggct tcgctgtcgc tgcaggcgat gtcggacgcg
    23221 atctgggtca gtccgtgcgc gatcggaagc aacgcgcgtg cgcgggtggg cccgctgtcc
    23281 acggtcagct gcaacgatat cctccgcgag aggatctgca tctcgatgtt cgcggtgact
    23341 tcaccatgtg ccggcgcgtc ccccgactgg gcttcgggtc ctgcgggagg acgcatgtcg
    23401 tccgacgacc tcatggcgtg gtccatggca ctgcgcgggt atcggtcgaa ccgtgcaact
    23461 cacgcgcttc catcatggcg aaggtgtcgt tgcgggggcg ggtgcatcgt agtgcagcca
    23521 accgggcggc accaagggtc gggatcgctg gcgccggggg gcgcggcagc gcggcgactg
    23581 caagcgcgtg ggcaggtgtc ggcagcgatc ccgctccagg tcatcgcttg cccacgcccg
    23641 gcaaggcggt gatctgccgc atccatgcca caaccgggtg tccgtcgatg tcgtcgagcg
    23701 aggcaagctc gacgccgcgt gtcgacttgc ccatcgccac cggcgtgacc ggcggcaccg
    23761 ggtccagcgt gacgccgttg acgaacatca gcttgacgtg gccggcgaac gccccgcagc
    23821 tgaagcacca gccctggccc acgccgtagt acgccatgcc ccatttcacg ctgcgctgca
    23881 ggccgggcag gtggtccgca gccaaccggt cgattcgttc ggcgatgccg cgttgcggct
    23941 gcggcaggct ggcgatgtag gcgaacacgg gcttgtcgcc gacagccgcc ttggccgggc
    24001 tggccttgcc cgttgtcatg gagtccggga gccggtttgc cggtgacgac gtgggtgtgg
    24061 cgggtttgcg ggtggccatc gtgtgctccg ggtctggtgg catggtgcat caggcgacga
    24121 gcgggtgcga ccgggatcga cacaggcatg acatcggcgc agattgccgc gcgcgcgcgc
    24181 ttggcggcta ccatgcggac cccacctcca tggaactgcc gatgtccctc tcgatgcacg
    24241 ctgcctccgt tcccgtgttc cggcagatgc tggccgcgct ggcgggcgtc ctcgccaagg
    24301 cggaaaccca cgccagcgaa cgcaagatcg atccggtcgc cttgttgcaa gcgcgcctgt
    24361 tccccgacat gttcccgctg gcgcggcagg tgcagatcgc ctgcgatttc gctggcagcg
    24421 tctcggcgcg gctcgcgggc gcggaggtgc cggcctacga cggcggcgat gccagcttcg
    24481 ccgagctgca gcgcaggatc gacgcgacgc tggccttcat cggcgggctg gatgcggcgg
    24541 cattcgaagg cagcgagcag cgcgagctgg tgttgcgccc cggcaccccg aaggaacgcc
    24601 gcatcggcgg acaggcctac ctgctggctt acggcctgcc gcaattcttc ttccacgtga
    24661 cgaccgccta tgacttgctg cggcacaacg gcgtggagat cggcaagaag gatttcatgg
    24721 gcgcgtactg acatggaggc cagcgattcc cccttgctgc tcacggccgc cgaagcgcgt
    24781 gcgctgggct gcctgatcga gaaagaagcg accacgccgg atgtgtatcc gctgacgatc
    24841 gccgcagtgc tggcggcggc caaccagaag accgcgcgcg agccggtgat ggcgctggaa
    24901 accggtgcgg tcaaccacgc gctgcgccag ctcgaagcga aagggatggc caggcaggta
    24961 ttttcctcgc gcgccgagcg ctatgaacat cgggcgacgc agcacttcgg gctgacctcg
    25021 cagcaggtcg ccctgctggg cttgctgttg ctgcgcggtc cgcagaccgc gggcgaactg
    25081 ctggcgcgca gcgaacggct gcatgccttc gccgatgtgg aaggcgtgcg ccaccaactg
    25141 gagcggatgg ccaggcgcga acctgcgctg gtggtgcaac tgccgcgtgg tggcggccag
    25201 cgcgaggacc gcttcgccca cctgctgtgc gggccggtgg acgtgtcggc catcgccgcg
    25261 cgcgagcccg gcggcgcggg cgaatcgtcg tcgcccgggc tggcggcgcg cgtggcggcg
    25321 ctggaagccg aggtggcggc cttgcgcgaa caactcgagc gactgctgca ggcgacgacg
    25381 caggagacgg ggacagcgac ctgacgccca agcggccgtc gtcgcagtgc tggacgaccg
    25441 ccgcccggtg ttcgttttcc ccggcgacta caggttcgtg gcggggccat tcaccccggc
    25501 atagccattg gcgcagccgg tgctgcaggt cgtggtcaac atgttgcgga cgatcacgcc
    25561 ggtgttggcc agggagcgga agtggccgac cccattgttg tcgccgccgc cggcattgaa
    25621 cgggatgccg tcgctccagt tccagtcgta ggcgtaggcg ctgctgccga cgcccacgtt
    25681 gacctgcgcg atgacattgc tgcgcgcgtt gaacaacgcg ccgttcgggc agctggcggc
    25741 gttgctcgag gtgaagcagt gcacctggtc gcgcagtccg gcggtcagcg tgtagaagcg
    25801 gatcgccgtc cagctggcgg gcaggctacg catcgagcca ctgccgctgc cggtccagcg
    25861 gttgtaaccg tagtagagca cgccgctgct ggggtagagg ccgaacgtgt agtagttgta
    25921 cggatagacg taagcctcgg cgttgcaggt cggtgcaagc gcgctctggt agccggtgta
    25981 cacgcaggtt tgcagcccgc ggatgccgcc gccgatgttg acgaagcgac gcacgctgcc
    26041 ctggtagccc ttgtagtgga tcatcgccag cgccatcgaa ctgccgagtg agtgcgccac
    26101 gatgtccacc tggctcttgc cggtgtatgc cttcaccttg tcgatgaagg tcttgatcaa
    26161 gtcgtaggtg tacggctcgt ggtagttcac ctgcgggttg gcgcgttcgt cggcggacag
    26221 gtacgtgacg ccgaacagtt cgcagtcgtt gtagccggca gccttgagtt cgtcgtattg
    26281 cgagcgtgcc ggcgtggtgt agcccgctac cgcgccgggt ggcatgtcga aactgatggc
    26341 ggtgtcgccg ttgccgtgca ggaataccac cggcgtgcgg gtggcggtgc acgtgccgcc
    26401 accgaagccg ccatagccga cgccgggagc gaagccgccg gcatattggg cctgtgtgcc
    26461 ctggcacagg tagccattgt tggtcccgca gtcgagcgcg gcggcggagc cgctccacgc
    26521 ggatgcgagc aggaggcttg cggcgaggat gcggcgaatg cggtggaacg gttgcaggcg
    26581 tcgagcgaga tgtggcgtgg tgtccatggc atgccctccc aggcgcctga tgtccgctgc
    26641 cgatactccg cgtgtgcact gcaccaaagc actgtacgaa agggcagtgc gatgctgcat
    26701 cgcaagaggg gccgggacga cgacatcgcc gcacgccggg cgtggcgatt aagctgcagc
    26761 ctccccattc acgcccaggc aatccgatgc gaatgctgct gtcccgctgg tgctgcattg
    26821 ccgtgctgct ggtcggtccg atccaggccc cggccgcctc ggcatcgccc gaatgcaagg
    26881 cgacggtgac cggcgacctg cgcgtgcaca cgctcaggag cgcgatcttc ggcaacgaac
    26941 gcaagatccg cgtgctgctg ccagacggct atgcagatgc cgccaacaag gatcgccgct
    27001 atcccgtcct ctacctgctc gatgggcaga atgtattcga cgcctgcctg agtgacgtca
    27061 gccaccacga atggggtgcg gacgaagcgg tgaatcgact ggtcgccgac aacaagatcc
    27121 cgccgatgat cgtggtcggc gtggaccacg ccggcatgca gcgggcgcac gagtacctgc
    27181 cgtacaagga tttcgtcggt aatcccgaca tggaggagcc ggcgggcaag caattccccg
    27241 atttcatggc gcgcgaagtg ctgccgctgg tcgatggcca gtaccgcacg ctgcaggggc
    27301 cggcgaatac cggcatcggc ggttcgtcct atggcggcgt cgcgagcctg tacgccatgt
    27361 tggccaagcc gaatgtgttc ggcttcggcc tggtcgagag tccggtgctg tggatcggat
    27421 tgggccagct ggtgcgcgat accagccccc tggtggtgat gccgcgacgt gtcttcatcg
    27481 gcctgggcgg acaggaatcg tccgatgcgg ccgtgaacag gaaaatgctc ggcctggtgc
    27541 gccaggtcga ggcgaatttc cacgccgccg gctacgacac atcgaatctt cgcgtggtgg
    27601 tggacgccga tgcccagcac accgaggccg cgtgggagca gcgcttgccg agcgcgctgg
    27661 cctttctcta tggcatccag gtgactccgc gtggagaccc gccgcgttga ccggcacgac
    27721 gctctggatg gggcgcgttg tgcggcccgc gccatcacga catctccacc cgcgcgggtg
    27781 gacagtcgcc ccgtcatcac gcacaggggc accaccatgt cgcaatcctt caccgccggg
    27841 acgcgccgca tgcaggacgg cagcttcctt ccgttcgtgg acgtggtcga cagcgacctc
    27901 ggcaccgaat ggcaacagcc gcatccgcag ggttgcgtcg cactggaagc ggaagccgaa
    27961 ctgcgctccc tgcaattcgc ccaggccttg cgcgagggcg ggccggtgga aagccccgcc
    28021 cagcgctatc gccagtcgag cgcggccgcg acccagccgg agcagcctgc cggcgcggtg
    28081 ttctcgcgct ttgtcgccca actgaatcgc caccagagcc tgctgtcggg cgcggagcgt
    28141 cggacctcga tggccgaggt cgcctgatat cgcggaccgc gctcagccct ggtcgctgcc
    28201 ggcgatgcgg ttgatctcca ggttctccac gacgcggggt ggttgcggca tcgccgccaa
    28261 ggccagcagc gtgcggccga tcgcggcagt gctggtcatc aggcccggca gcaggcgcac
    28321 gcccagtccc atcattgggc gtcccagtgc gtacatcggg cgcatccatg catgcggact
    28381 gcgttcaccg tgggcgggtt gcacgccgcc ggggcgcagc atcgcggtcg tgatcggcag
    28441 cgcctgcagc gcagcttccg cctcgccctt cacccgtagc ggcatcagcc tgctgcgcag
    28501 gttggcgttg gcgcccgaga tgtacaggaa cctggcctgc gggttgcgtt cggcgaaagc
    28561 gcgcgccacg tgcagggtca ggtcgacggt cacgtgccgg tactcgcgct ccgcggtacc
    28621 caccggcggt gcgcccgcgc aatagaagca ggcatcccag ggcgcgagtc ggtccgagac
    28681 cgcatcgagc gcatcgaatc gctcgaccac cagcgattgc acccggggat cggcgtgttc
    28741 gaccgggcgc ctgccgagca ggccgatgtg ggtgacggca gggtcagcca ggagctcgtg
    28801 gaggacgcct tgccccacca gtccggtcgc gcccgtgagg aggatgcgca tgctcgtggt
    28861 ccgtcagtgt cgttgtgggg agtatcgcca tgcggggagc ggtcggccag cgccgcggga
    28921 ttgcttcacg gattgcgcat cggcgcgatc gcgtccgccg tagtggctga gccgggagcg
    28981 aagacgcatg cgaacacccg tggagggcct ggatgtgcca gcgcaggtgc tggtgaccgg
    29041 cgccagcagc ggtattggcc tggcgatggt cgaggccttg ctggacaacg acggcgtggc
    29101 cggcgtctgc gcggtgtccc gccatgcgct ggcatctccc gcattgcagg cactggcggc
    29161 cgggcatccc gggcgactgc atcgcatcga tgccgacttg gccaccgatg ccgggatcga
    29221 ggccgtggcg gccggtgcca gccaggtgac ggatgtcctg cacgtggtgg tgaatggcgc
    29281 gggcctgttg catggcgatg cggtgcatcc ggagaaatcg ctggccacgc tggaccgggc
    29341 ggcgctggag caggtgttcg cgttgaatgc gttcgccccg atcctgctgg cgcgtgcgtt
    29401 gctgcccctg ctggatgcgc gttcgcgctg cgtggtggcc tcgctgtcgg cgcgggtggg
    29461 gtcgatcggc gacaaccgcc tcggtggctg gtatgcctac cgcgcagcca aggccgcgca
    29521 gaaccagttg ctgcggacgc tgtccatcga actgcggcgc agccatccgc tcgccacctg
    29581 cgtgctgctg catccgggca ccgtggacac gccgctgtcg cggcccttcc agtcgcgcgt
    29641 cccggcggag gcgctgttca cgcctgcgcg tgccgcccgc cagttgctgg acatcatcgc
    29701 gggattgcag ccacgcgaca gcggtcgttt catcgcctgg gacggcaccg acgtgccgtg
    29761 gtgatggcct cgcgtcaggg taggtggaac tccgtggaca gcctgtcgaa catgggtttg
    29821 gccagcagga agccctggaa cagctcgatg ccttcgccgc ggaaccacgc atattcctcg
    29881 acggtttcca cgccctcggc gatgaggtcg atgcccaggt cgaggcaggt gcggacgatg
    29941 ccgcgcagga tcgcctggcg cgggccattg cgctcgatcc cgcgcaccag gttcatgtcg
    30001 atcttgagca ggtcgggctg gaaatcggcc agcaggttga ggcccgcgta gccggcgccg
    30061 aagtcgtcga tggcgaaggt caggccagac gcgcggtacg gattcatccg cgccgagaag
    30121 tcgccggagt cgcggatgat ttcgctctcc agcacttcca gcacgatcga ctcgggggga
    30181 atgccgcatc gcgctgctgc gtccagcagg gatgtgatcg ccgtcggcga gtgcgcgatg
    30241 tccgatggct ggaaattgat gttgatgcgt gtgcgcaggc caagtcgcgc cgccgattcc
    30301 agcgctgcga tgcgttgccg ttcgcgcaat gcatgcattt ccactggctg gctgcgctgc
    30361 aggatcgacg cggcgttctc gccgtccggg ccgcgaatca gcgcttcatg ggaaaagatc
    30421 gaacgggtgg gcgcatgcac gatcggctgg aatgcgaagg cgtagcgcag cgtcgccgcg
    30481 tcttcgactg ccgcgatcgg accggccggg gccgtccgcc agcgcccgtc gcggaaggcc
    30541 gccagcagtt tcttggcacg cccgtcgccg atctcggcca acgagcggcc gcccgcgaag
    30601 aagtcgttgg cgccgacgat cccgtccagt tcatgacggc tcatttccgc gtggcccatc
    30661 gtccattcgc cgaacgcgcg gaccgccacc ggttcgcgga tgatcacggt gacatggtcg
    30721 tggcgcgggt ccgcgccaat ccgcgcgaac aggcgttcga catcctccgg tgcgccctcc
    30781 agcacctgga agaaactgcc gtcgacatgc agcagcatgc cggtgatccc gtggcgttcg
    30841 ttgtacgcgc gcgcgccggc aagcagcgta gcgagttcgt cgcgacccag tggccgcggc
    30901 gcggcgctgc agtagatcag gtggatcagc gggttcacat cgcgcctcgc caagggagcc
    30961 ccgacggcct gtccacggca ggatacgccg cgattgcgct ggaaaccgtg ccccgcggca
    31021 tgcctagggc gagtcgtgca ggtccggcgc cggcacgtcg atggcggggc ggaactggac
    31081 caggccggtg tccttcatcg tccgttcgat ctcggtggtc gtcgcgggga cgaaggcgct
    31141 caggttctcc gcaccgtcct cggtcaccac caccacgtcc tccatgcgca cgtacaggcg
    31201 ctccccatgg atccagatca tcgggtcgat ggtgaacacc atgcccggct gcagtttcac
    31261 gccgcgcaca agcccgacat cgtggacggc catgcccacc ggatgctgga aatggccacg
    31321 gaacttcagg ctgtccgtca ccgcttgcag gtgcgccgga ttgtctaatc gcttgccgac
    31381 caggtagcgc gccatgtcgc gcgccgccga atcgagcacg aaatccgagg tcacccccgg
    31441 gcggatatgc cggagcagcg catcccggta cgcgaggatg aagtccgaca acgcgcgctg
    31501 tcccgcgctg tacgcgccgt tgaccggcca catgcgggtg acgtcgctgg tgtagtagcg
    31561 gtagtcgggc gcgtagtcca tcaggaccat gtcgccgtcc ttcagccggt cggccttgcg
    31621 gaagtagtgg cccaggtacg cattgctgcc gccgccaatg atcgaggcat agccgtcgcc
    31681 gcgggcgccg tgcaggtaga acacgtactt cgccgcggca tccagctggt attcgaagac
    31741 gccgggttcg gtggagcgca tcgcttcgat gatcgccatc cccgcgatcc gggtggcgcg
    31801 gcggatcatc gcgatttccc gcggcgactt gatgttgcgc atcgcgtcca ggatcggcga
    31861 caggtcgcgc acctcgaact gcggcacccg tgcctgcagg agcgcacgga agcgcgcctc
    31921 gcgcgatgcc aggccatccc acgggttgga cgagacccgc gcctgctcgt tcaggagctc
    31981 gtcgcggctg tcggtcccgg tttccgccgg gctcagcggc gtgaacagct gcaacgcggg
    32041 cggacgcacc aggcccttgc cgaccaggtc gagcgacagc gactcgatgc tccgcaccgc
    32101 atcgacgccg gtcagcgact tgaccagggc ctcgtcctcg acggagagga tcttgccctc
    32161 gttgcgttcc cgctcggcat cccggtgcgg caggtacaag gtcgcggtgc ggctgcgccc
    32221 gtccagcatc aggtaggcgt gcggcgtctc gatgccgctc aggtaataga actcgttcga
    32281 ctgccggaag atgttgaagt cggcggtgcc tttcgcgcct tgcacgaggg cgatcgcgtt
    32341 gtcgccgatc ttcgcgaaga ccgtcgcccg ccgttgcagg aattcttcct tcgggaagtc
    32401 cgcctggtag tagtgcgtct cctgggcgcg gaccacgctg gcgctggccg agagcaggac
    32461 cacgaagcct gcggcaatcc atcgtgtctt catgcggtgt tccaggcaac gggacaggga
    32521 cgccacgtta cttcaacgaa ccgtgtcccg cttggccggg cgacaaggcg gtttcaggat
    32581 gaatcgcccc ggatgcgctg tacccgcttg cggtagtcgc gcaggttgtc gccctggatc
    32641 cgcgactcgc gcgtgccctt gatctcgtcg accttgcggt cgcgctccgc gccggtgacc
    32701 ggcttggcat cgaccacggt ctcgcgctcg aaggcgtggg acttgtccgt gttgcggtaa
    32761 tagcgataca gcatccagta caggccggca ccaccggccg ggccgagcag gagcagggcc
    32821 aagccaccat cgtcgctcat gtggaactcc ccaacatcca gagtgcaatg ccttccagga
    32881 aggtgccgac ggtgatcgcg gccagcagca gtttccattg ctgtaccggc acgctgccca
    32941 tggtctcgcc ggtgcggccg ttgaccgcga tgtagtgcag catcccgccc ttgccgccct
    33001 gctggtagga gtacagccac acgggcaggt acatcgccac ccagcgggtg ccgtgcacgt
    33061 ccaggcgttc ctgttcccag cgcacgccgc gatcgaagcg gttcaccgat tcctcgacct
    33121 ggcagcgggc gatcgacagc aactggtcct ccaggcgcgg gcgcaactgc tccacgtcgg
    33181 tgtcgcgctt ctccgaggtg tagccggaga ggtaggacgc attccacttc accgcgttct
    33241 tggtgtcgaa cggcaggatc gtgttgatga tgttgttggt gttgacgccg gtgtccaggt
    33301 tgccgcgttc ggtggaggac tccagcggca ggtcgtcgac ggtgaagtcc acgtgccgtt
    33361 ccacctcgta gatgtcggcg tcgtagtagg tcttcttctt gtcgccgctg ccctgggtgt
    33421 actcgcgggt cttgatctcg ccgcgcccgg ccacgtccgc gctcgccttg ccgtccacga
    33481 tcatgtaggg caggtacacg ccgaccacgt tctccggggt gaactgttcc ttgaattcct
    33541 tcagcgcgaa cagccgccgc ttgtccacga actggcggat ccgcgcgacc gcatcgtcct
    33601 tcttgatgtg gaagggcaac accgcgtcgg gcaccgcgcc gttcgccact tgctcgttga
    33661 cgccgaacac gtggcggcac cagtggcagc gcgcggtcat cgtgctttcg gtgttgaccg
    33721 tgacctcggc gccgcagccg gtgcacttgt aggtcatcag gctggagaca tcggtgtcga
    33781 tgtcgcgtgc accggaggcg atgaccgtgc cggtcagttc atccaggccc tcgccgaagc
    33841 cgaattcctc ctccacccgt tgcccatgcc actcgttgcg gcagtacagg cagatcagca
    33901 ggtcggtgcc cgggcgctgc ctgatgtcgg tggcgccgca cttgggacaa cggttggcgc
    33961 cgtccttcag ctcctccgcc gcggtgtcga tggcgaccgg atcaggcgcc agcaattcat
    34021 cacggatggg gtcgggcagg gtggcggggt cgatggggta gctgcccggc accggtggga
    34081 cgtcccgcgg cgagcccggg ccggtgggtg gcaccggcgg tggtgtggtg ttggacatgg
    34141 tggctgcgcg atccgcgggt tacaggccca gtgccttggc cttgagggcg tcgtaatcgg
    34201 cctgcgagat caggcccagg tcgagcatct ccttggcctt cttcagtttg gcgatcggat
    34261 cgtcggccgc cggtgcgggc ggtgccaccg gctgttgcat gttgccgatg cccatcatgc
    34321 cgctggccat gcccacgccc atcaggcccg ccgcgccgcc gttctcgccg gccgactgga
    34381 tgccctgggc cacgctggcc tgcaggttgg aattgccgcg cgcgccggac agcgcatccg
    34441 cgcgctgcac ggtcttcagc agttccttgg tgttggcgtc gtattcgatc gagacgatgg
    34501 cggtcttggc gatgaccagg ccgcgatccg acttccactg gtagccggcc tcgaccgcat
    34561 ccgacaggct cctggcgaag ccaatcgaat cctgctgcag cttggtgatg cggttgccct
    34621 tggacgggtc gttcgtgtac aggctgaagg ccggcgccag cgagcccacc acctcgttga
    34681 acagttgcgc cgcggcggcg ttgtcgaggt cggtgaaatc gaagaccttg ccgggctgca
    34741 ggtagctggc cggca
//
LOCUS       DAOOCX010000007        34072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_953_length_34072_cov_8.295147, whole genome shotgun sequence.
ACCESSION   DAOOCX010000007 DAOOCX010000000
VERSION     DAOOCX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 34072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34072
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_953_length_34072_cov_8.295147"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..722
                     /locus_tag="PK399_01250"
     CDS             84..722
                     /locus_tag="PK399_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014509279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPW11785.1"
                     /translation="MACGEPGQEGRDLCGACHADLPWQGPACLRCALPIPLAGTCGHC
                     LQSSPPLGEVHAVFDYAFPLDRLLPRLKFHRDFAAGRVLAQCMADRLAALPRPGAMVP
                     IPLHRARLRHRGYDQALELARPLARALGIPLVDRALGRRKTTRAQSRLDADARRRNLR
                     DAFHVDAGIPLPPHVVLVDDVMTTGATLHAAAGVLHRAGVARVDAWVCARVA"
     gene            complement(730..1653)
                     /gene="ubiA"
                     /locus_tag="PK399_01255"
     CDS             complement(730..1653)
                     /gene="ubiA"
                     /locus_tag="PK399_01255"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HPW11786.1"
                     /translation="MPNDRDFAEQPRIEPLWRERLRQYWALVRGDRPIGWLLLLWPTW
                     WGLWIAAKGMPPLWPLLVFSLGVWLTRSAGCVINDYADRWLDPNVERTKQRPLATGAV
                     SGQEALAVFAVLMLVAFALVLTLNRLTVWMSVVGVLLAASYPYLKRYTYLPQVYLGMA
                     FGWGIPMGFAALTGEVPPVAWVLYVANIFWATAYDTWYAMVDRDDDLRMGAKSTAILF
                     GDMDLVAQGVLYTCMFAALALVGRDAGLGVYYWGGLGVALLLVAYQFGIARGRERAAC
                     FRAFLHNHWVGMAVFVGIAVDFAMRGTAVAA"
     gene            complement(1903..1979)
                     /locus_tag="PK399_01260"
     tRNA            complement(1903..1979)
                     /locus_tag="PK399_01260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1943..1945),aa:Arg,seq:ccg)
     gene            complement(2098..3732)
                     /locus_tag="PK399_01265"
     CDS             complement(2098..3732)
                     /locus_tag="PK399_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010375091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HPW11787.1"
                     /translation="MKAQMSLLSLAIASGLALAAIPAEAAGNLKSAAISRANVLLGAN
                     GTGIIRGAKGDVFAARDAIVDADGTEHVRFERSYRGLPVIGGDFVMHSRNGQLRNVSQ
                     SLKSAARPNLVAQFGSDEAIVEAGARFGSKFFGAPTSRMVVYARGASPVLAHEVVFNG
                     IKADQTPTEMHYFVDASSGRILDQWDTVHTAKPGPGGSGCTGATAAVGTGKSLTEGTV
                     VLNTTKCGSTYQLVDMTRGGGATNNMSLKTAGLGSVFTGTTNVWGDFLVSNAQTVAAD
                     AHYGVATTWDYYKNMHGRTGIANDGVGALTRVHYGRNYQNAFWSDGCFCMTFGDGDNG
                     ATILPLVALDIAGHEMSHGVTSRTANLIYSGESGSLNEATSDIFGTMVEYYANNANDP
                     GDYVVGEELFKNNANMSQAIRYMFKPTLDNYSQHCYDPATTPTFEVHGGSGIANSFFY
                     LLAEGAVVPAGFGAGTWANLTPSSLVCNGNTSLVGIGRTAAGKIWYRALTVYMTSGTN
                     YAQARAATLSAAADLYGAGSTQHNAVAAAWSAVKVN"
     gene            complement(3752..4339)
                     /locus_tag="PK399_01270"
     CDS             complement(3752..4339)
                     /locus_tag="PK399_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11788.1"
                     /translation="MKIEIVPVWKALSPELAAELVAFWRDQNAIQNEAAAARRTEQAV
                     CIARDADGALCGVATAVVKVLPRLRQPMYYYRQFFARALRGQHQELAFYRRAKQILQD
                     YNAGLDKPESLGILLEVENAKIAAAYKRAHEPGFDATFIGYSPRGLQLRVSYFEDAIL
                     QAPTPLGAPAAVPGTRRVAGTRPAPSIPRHGNGRS"
     gene            complement(4336..4785)
                     /locus_tag="PK399_01275"
     CDS             complement(4336..4785)
                     /locus_tag="PK399_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HPW11789.1"
                     /translation="MNTIRGTMMAMACGLVLLGAQPVAAQSSAAKPATPARPAQDPAA
                     EAFKAWDKDGNGSLSLVEFRTGWQQVQRVAEMQARLRHQFGTVDANKNNAIDPAEYGS
                     LVLVKQAGKNAPQMSVYDANRDGKLEFGEYVKLVQALAPEAGKAAAK"
     gene            complement(4782..5987)
                     /locus_tag="PK399_01280"
     CDS             complement(4782..5987)
                     /locus_tag="PK399_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HPW11790.1"
                     /translation="MHVTNENTQEEPQVQGHQDKPELDTGLDTVSADAPAPAAGEQKK
                     PLDITKLKVEKSCSRGLAGWLAQHRLSLAITSYQTGRIYLVGSDQKGRVSFFERIFER
                     AMGVVGNAQRIYLGGLYQLWRFENVLRPNEVIHGQFDKCYVPRNAQTIGDLDIHELGI
                     RKNGKVVFVNTKYSCLAELSQTHSFKAIWKPTFISKLAPEDRCHLNGLAMVDGEPKYV
                     TAVCRSDSVDGWRDRRQDGGVVIDVQTDEIVCEGLSMPHSPRWHNGRLWVLNAGTGHL
                     GWVDFEKKAFVPHASVPGFARGLSIIGNVAAVGLSKPRNQRFEGLQLDGELKKRDAEP
                     WCGVQIISLANGDVLNWIRFEGDISEIFDIAFLPNVKNPMMIGLRTAEIRDLITFESD
                     LPAAEASAA"
     gene            complement(6045..12065)
                     /locus_tag="PK399_01285"
     CDS             complement(6045..12065)
                     /locus_tag="PK399_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11791.1"
                     /translation="MTRAVRSALAVSAMALALGASGGAFAAGPKAPQAQVLQVQHVTL
                     DFAPVHDLATVAPPIWMEVPPELHPTLISEYAIGDVVIDNADPIDDVNIYYSATAISG
                     YSSEGNVDITNHVGADLLAASLGGNAIGIYGYATIGDVSIDNAADIFALSAYYVADGI
                     FASGANVDVTNSGAIDVYGYSWAAGIEAQGTGTVHVDNSGDIYAEVLDAGTAFGIYVT
                     GADVTVDNSGDIEAQGYSGTGIEAQSSGDLQVTNSGDIVGGSLTAVYDYYSGYTYLYG
                     SLVGTGINAISNGEGAAVGVTNSGDISSQGYYGATGIAATSGGLGGSASVENSGNIYV
                     SQYNKYGYGAYGIVVSADGDASIDNQAGASITVDSAGVANGAVALSFAGDASVTNAGD
                     ITVSTDAFLYYGANGIVAFAGNGSAYAGNSGNIDVTSKYISSGIDVSGLEGATASNSG
                     DISVDAWRAYGIRAQAGNGDVEVSNEGSIYATYGASGGTAFGILGISGNGDVTVDSSG
                     SITTAVTGQSVGIFGLAYAGDVNVSNSGVIDAYSYDGAALGVFARADYGTATVDNSNT
                     ISAYSYYGQAYGILARGAYSSVSNSGDIEASGFYAAAGIIATSYYGTTVTTTGGSISA
                     IAVGEGLGIDARSFYGDVSVDNASDIDAVGIVLGARGINAAAYDGGMVDVANSGDIYA
                     GSLYGNSIGIYGYSVGGDVSIDNSGDITAISYYALADGIFASGANVDVANSGAVEVYG
                     YTWAAGIEAQGDDSVVVDNSGDITSTAPGLYQVYAYSFEYYAGGTIANSPGAQAFGIY
                     ATAGEGGATVGNSGNIEVQGGYVTGIEVQSGGDISVTNSGDIVAGEGLQSAAYYNPDT
                     YTAYYVYSGTQVATGINATSNGEGAHVAVSSSGDISADGIFGASGIAATSGGLGGTAS
                     VDNSGNIYVSQNQKYGYGAYGIVVSADGDGAIDNSGLVDVYSGGVANGLTALSFAGDA
                     NVTNSGDIVVENTSSLYYAASGIVAFAGNGNANVDNSGDIAAVSSYWATGIDARGYGD
                     TTVENSGSVHADGQKYAFGVYATAGAGDVSVTNADGGDIYAYSYIGRAWGVFGYAAQG
                     DVHVVNDGSIATYAYGQSAGVFVRSGEGDVGVTNTGSIEAITGGNVAVGIFARADYGT
                     ASVGNGGDIVASVEGVNGYIGDDAYGILAIGEYAQVANAGSIYGFGIYSATGIAARSL
                     YGTTVTTTATSDIEAYSLLVTVGIEGRSEYGNVSVTNAGDISVEGVLGGGVGIQAYSG
                     LGNASVGNSGEIAASSEYGTAIGITAYSVAGDATVTNSGTIEATGYYGAYGIAAQSYL
                     GDVTVGNSATGHIHAAAPSYAYGILANAYGNVVVNNAGAVSASADAGGHAAAVLMESM
                     YGTSTLNNAATGMISVTGTDGYAFAVLGSDAVDTINNNGHITGALALYGGNDVFNNRS
                     GGVWSTGGTHSTDFGDGNDTLNNLAGGLVDILGGAITFGAGNDTLANAGTVRTGTGGT
                     ISFGDGNDTLSNAAGGLVDLVGGTVAMEDGNDTITNLGTFRLTGGTVTMGAGTNAFTN
                     ISLLKALGNSTVDAGAAGTLTNVNTIDLLDGATDDRLTVVGTLAGTGALNLDLNMLNS
                     TVDQLVVTGNVAANAVQTVNVAAPGLPTTAHTSAVFGRVSGTSTASNFVAGRIFGYSP
                     ANFLDLDLTVTSQINTANTAADLFSINVDVAGLSDSGSLAASIGSGAADFLNSQVGTF
                     RQRLGVNPYGDAGKVMSAFVRVYTSEGDVDQAHVAANFGQGGNFAYDLSSWGREVGVN
                     ANLFGNFHAGVVLGTADSRQRLTGAGVGQNRMDGMTWGAYATWYVPQGFYVDLTHRQM
                     AVDVESTSIAGLLQSRAHTTSKSLEAGYEWAVGGFNIVPQVQYTRTDVEDIRAIAFDR
                     ATFEGHGGTSERGRVGVEFNKTFTAGNLRWTPYGSINAIREFDGEMEYTVGTNFFGST
                     STEGTSAMAELGLGVQSGGWGFTIGANWADGGAYKSTVSGQANIRFAW"
     gene            12557..13078
                     /locus_tag="PK399_01290"
     CDS             12557..13078
                     /locus_tag="PK399_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPW11792.1"
                     /translation="MRNYDLEFLKRFSMVLAFLALVTLGLILFAHHLNSKVVHEVDPA
                     THQRLLARIAPTGDVYAGATGAAAQAAAAAAAQASLLANIPFDGRTDGAEIFNNSTCT
                     GCHTAGVGGAPKLEPAGIGARLAEQGLDTLVSKAISGFQGSAGVMPAKGGNPALTDEQ
                     IKATVEWMVAQSK"
     gene            13187..13867
                     /locus_tag="PK399_01295"
     CDS             13187..13867
                     /locus_tag="PK399_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW11793.1"
                     /translation="MSESPAAPASADLPLAIAGPAGRIEAAFDLAEADATPQPVLAIV
                     CHPLPTEGGSMHNKVVTMTARALRESGIATLRFNFRGVGQSEGRFDDGRGELDDLRAV
                     AAWVRVEHPDKALWLAGFSFGAWVALRGAVELGAAALISIAPPVGRGWSFDGVAMPAV
                     PWLLIQGEADEIVDAGAVFDWVATLPRPPVVVKMPDTSHFFHRKLIDLRGAVRHGVRD
                     WLPVPPAA"
     gene            13864..14961
                     /gene="zapE"
                     /locus_tag="PK399_01300"
     CDS             13864..14961
                     /gene="zapE"
                     /locus_tag="PK399_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HPW11794.1"
                     /translation="MSDPLLPSRRYADGVARGDWQYDPAQQPALLALDRVHAALQAPV
                     PTRGVFQRLFGNPPPPAPRGLYLWGGVGRGKTFLIDLFFAGLPFPEKRRTHFHRFMRD
                     VHQRLRDHAGERDPLVAIAREWRRSVRVLVLDEFFVSDIGDAMLLGRVLDRMFAEGIV
                     LVTSSNAAPTDLYKDGLQRARFLPAIALLQEHCAVVELVSDTDYRLRALTRSPVYRAP
                     LDAGSDAWLQQRWRELGGDDGHVDAGIIIDGRRIPVRARSKGLCWFDFAALCEGPRGN
                     ADYIEVAREFHTVLLGGIPRLDGNRDDAARRFVHLVDELYDRHVNLVCTAEAAPMDLY
                     AGERLVAAFERTASRLVEMQSTAYLAHEHGG"
     gene            15027..15479
                     /locus_tag="PK399_01305"
     CDS             15027..15479
                     /locus_tag="PK399_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11795.1"
                     /translation="MTSIPRIAGSALLLLALAACNRQGPDVPKPVDDNKEFRDQADGI
                     ESVVVSPDAAKVGSEVDGAGDIANEVRTFDAGQKVYISVPAKFGRKIGDKLEIFWFHD
                     DNRSRHEEQKQIAGPFTVFEFAPTDVGAYNVEIAANGRPIALVQFEVK"
     gene            15497..16309
                     /gene="queF"
                     /locus_tag="PK399_01310"
     CDS             15497..16309
                     /gene="queF"
                     /locus_tag="PK399_01310"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017171998.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HPW11796.1"
                     /translation="MGDIDHSPLGREVAYPRHYDASLLYPIPRALGRAAIGVGAPLPF
                     IGVDRWNAYELSWLDPGGKPRVATATLRVPADSPHLVESKSLKLYLNAFNATRFADAE
                     AVRARIEADLTQAAGAPVAMAFGLPPFEDAVDAESIDALDIAIDAYDAPDAALLVADA
                     GDVVDEALTSALLKSNCPVTGQPDWADVTLRYRGPRIDRASLLRYLVGFRDHAEFHEQ
                     CVERIFVDVLARCRPHWLSVEARYTRRGGLDINPWRATAGIDAPTALRTLRQ"
     gene            16402..16725
                     /locus_tag="PK399_01315"
     CDS             16402..16725
                     /locus_tag="PK399_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPW11797.1"
                     /translation="MSNDKTPDFSNVQGGVQSTEQLKPDFSNVESKVTSTEAILGGEG
                     GGGSTGERTYTVEKGDNLSTIAKRFYGRQDWKRIYDANRDQLDDPDRIKPGQVLKIPR
                     DDQQD"
     gene            16773..17267
                     /locus_tag="PK399_01320"
     CDS             16773..17267
                     /locus_tag="PK399_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11798.1"
                     /translation="MKTNRLQMAMLAAMIGSAALVGCKKKEEPAALPPVATEPAPTAP
                     APAPMPATASVSAVDLGNAVGADMRVTAPMSSFAPKDTIYAAVSTATSDPAATVPGKL
                     GVKWTHVDSNQTVHEESRDIGFAGSGVTDFQISKPDGWPTGKYKLEVSLDGSVVQTRE
                     FEVK"
     gene            complement(17414..17965)
                     /locus_tag="PK399_01325"
     CDS             complement(17414..17965)
                     /locus_tag="PK399_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11799.1"
                     /translation="MRRAILLPGLLAMALAGLPGQATAQQPPANRPKPCSQAMHRQFD
                     FWIGEWTVSDPGGKPVGSSRIEATLDRCVIHEHWSGAGGSNGQSFNIYNTATRQWEQF
                     WVDNGGNRLHLKGGLVDGAMVLRGTQDVANAQTGLTQHERITWTPNGDGSVRQLWETS
                     TDDGKTWTVAFDGIYRRAATPSP"
     gene            18133..20358
                     /locus_tag="PK399_01330"
     CDS             18133..20358
                     /locus_tag="PK399_01330"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPW11800.1"
                     /translation="MTAQILYTLTDEAPFLATASFLPIVQAYAKTAGVDVATRDISLA
                     ARIVAQFNDLLPEDQRNSDDLRELGELAKTPEANIIKLPNISASVPQLKAAIKELQAH
                     GYALPDYPDEPQGEREKDIKARYAKAMGSAVNPVLREGNSDRRAPASVKAYARKHPHR
                     MGKWDGGSASHVSHMEAGDFYGSEQSYTMVNAGPLRIEYTSVTGSSFPLRGPLQVQAG
                     EVIDASVMSAQALADFIDAQIEDAKARGVLFSLHLKATMMKVSDPIIFGIAVSRFYAP
                     VLEKHPRTMAEIGFDPNNGIGDLYAKLGQLPEAQRAGIESDIAALYAQRPGLAMVNSD
                     KGITNLHVPSDVIVDASMPAMIRDSGKMWNAAGELQDAKAVIPDRCYAGIYQEVIDDC
                     REHGAFDPATMGSVPNVGLMAQAAEEYGSHDKTFKIAGDGTVRVIDEHGTVLLQHPVK
                     AGDIWRMCQTKDAPIRDWVKLAVTRARLSNTPVVFWLDPRRDHDRGLVAKVAAYLNDH
                     DTSGLDISIMSPIRAMRHSLKRIRQGLDTIAATGNVLRDYLTDLFPIMELGTSAKMLS
                     IVPLMNGGGLFETGAGGSAPKHVQQYVGEDYLRWDSLGEFLALAASFEHLANTTGNAR
                     AQVLADALDAANAKFLDTDKSPGRKLGTIDNRGSHFYIALYWAQALAAQTIDAELAAK
                     FAPLARALGDGEAKIVEELIAVQGKPVDIGGYYQPDLDKLSAAMRPSATLNAALATLA
                     G"
     gene            20474..21322
                     /locus_tag="PK399_01335"
     CDS             20474..21322
                     /locus_tag="PK399_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPW11801.1"
                     /translation="MPRFHASLLASGLVVAFAACASTTATPADTATATTQADAALDAA
                     IAHPARDPKNVARDVHRHPRETLGFFQVKPDATIIEISPSGGWYSEILAPYLRDAGRY
                     VAATASAPADSGGGKRNAALQAKFAAAPARYDRVQWLEYDGKAPMLGAPGSADVVLTF
                     RNVHNWVAAGTTDAYFQAFFAALKPGGVLGVVDHRARPGTGLEAMKTSGYLTEALVID
                     HATRAGFVLEARSEVNANPKDTADHPNGVWTLPPTNRHDPGDAAKYAAIGESDRMTLR
                     FRKPAR"
     gene            21406..21999
                     /locus_tag="PK399_01340"
     CDS             21406..21999
                     /locus_tag="PK399_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW11802.1"
                     /translation="MLIAFNKPYGVLCQFTDDAHGRASAARAGRAEAARSTPARPTLA
                     GFGLPPGVYPAGRLDSDSEGLLLLTDDGSLAHRLTDPRHKQPKVYLAQVEGDPCDAQL
                     QALRNGVQLNDGPTLPAQARRIDTPALWPRDPPVRMRKTVPDAWLELTLREGRNRQVR
                     RMTAAVGLPTLRLVRIAVGGHLLAGLAPGQWRDICAG"
     gene            22105..23229
                     /locus_tag="PK399_01345"
     CDS             22105..23229
                     /locus_tag="PK399_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPW11803.1"
                     /translation="MPHQIPRTGRILVVDDQAANVRVVTALLERHGYAVTAASNGKDA
                     LQAATGEVPDLLLLDMMMPGMDGFELLAALRELPGFRQVPTVFLTAAQDRDLLLRAFE
                     AGAVDYVTKPFMPEELLARVSAHLGLKLTRDRLERVARERQELVNLVAHDLKNPLTSV
                     LFATEMLALPDCKPERVPRYIEIIDGSTRDALGFIRNYLEGQERAASSKAAAVATPCT
                     WMGETLGWLAARYELQLEAKGLRLQVAPLERDASVAIDGLVLRQVAENLVTNALKYAR
                     DGGELELLARPGAPGFWQLVTQDRGPGIPPARQRELFKPFHRLTEVDPANGLSSGLGL
                     SLAKQIIGNAGGQLWYEDREGGGARFVIELPEAASSPSAG"
     gene            complement(23230..23586)
                     /locus_tag="PK399_01350"
     CDS             complement(23230..23586)
                     /locus_tag="PK399_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11804.1"
                     /translation="MSKLGNAQDRALELIGELGDGIRKAVPGKAMQWVETGAALGAVR
                     TGTRIAGKFVRRNPVLAGAAIAGAGLLWLAARQRAKKQAAGAPIEGTATRVEAKRGKA
                     SSRTRRKPPAAEQTAD"
     gene            complement(23741..26350)
                     /gene="hrpB"
                     /locus_tag="PK399_01355"
     CDS             complement(23741..26350)
                     /gene="hrpB"
                     /locus_tag="PK399_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HPW11805.1"
                     /translation="MSENAFPIQSLLPAIRGSLQDHPRLVLEAPPGAGKTTQVPLALL
                     DADWLRGQKIVMLEPRRVAARAAAGFMAKQFGEAVGETVGYRIRFENKVGPNTRIEVV
                     TEGILTRMLQDDPMLEGVGALLFDEFHERHLAGDLGLALALDVQSGLRDDLRIVVMSA
                     TLDGERLATFLDAPRLSSAGRSYPVEIAHFPARREEAIEHQTKRAIEQALVTHPGDVL
                     VFLPGQREIANVERLLAAALPAAGELLFPQSGHPAPIRARARHGAHDATPQPPTSQLA
                     DVDVLPLHGELPLEQQTRVLQPATDGRRRVVLATNVAESSVTLPGVRVVIDSGLAREP
                     RFDPNSGFARLDVVSISQASADQRAGRAGRVAEGFAYRLWPQSQRLEPQRRPEIAQVE
                     LASLALELAAWGSDALRFVDPPPTGALAAARDLLRRLDALDARHAITARGRRMLALGT
                     HPRLAAMLLVSNEPSRIALACDLAALIEARDPLRSRSDALAERWQALAAFRNGRVGAD
                     ANRSALAAIDAASKQWRRRLRCNAHPPADVPAHDLGDLLAHAFPDRIARQHPQDAKRY
                     QLANGRMAKLFDDSAVHGEPWLVASELRFEARDALLLRAAPVDEAYLRQAFAAHFHEG
                     DEVRWDAGRRALASERIARFDGIVLSSKPAGRVDPALAARALTDAVRDLGLTALPWTE
                     ALSQWRVRVQCLRAWMPELGLPDLSDAALLSSLDAWLMPAFAGKTRLDALDESALAEA
                     LKSAVDWNLRQRIDQLAPTRIDVPSGMQRAIAYALDDHGEPASPVLAVKLQELFGLAQ
                     TPRIAGGRIALTIHLLSPGGRPLQVTQDLAGFWERTYPEVRKEMKGRYPRHPWPDDPW
                     NAVATHRAKPRGT"
     gene            26486..28216
                     /locus_tag="PK399_01360"
     CDS             26486..28216
                     /locus_tag="PK399_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPW11806.1"
                     /translation="MSCAALALAFVAAPASADVFINEIHYDDATSSGDTGERIEVVAT
                     AGETLSAYRIYLYNGSTPSAAVTYDNDLVPAGSLVSCGAQVRIATVSYASNGIQNGPN
                     DGIALVNGAGQVVQFISYEGVIKASNGPAAGRTSVNLPVSESGSDAAGRSLRLTGSGT
                     TYANFSWQPAATASFGSCNAGQTFAAPNPPPVVTSTTPVAGATDFPAAGNLAVGFSES
                     VSLASGAFTLACSTSGNVALTHATSGASFPISTNTALHGGETCTLGVVASKVTDAGGA
                     KPVANTTVAFTVAAAGPTGYYSHVNTSSPSQLRCSLHDTIKGHTAYPYSGGTTNTWTI
                     LEIADEDPNNSGRILDAYRNRSYLKVTERASSGSGLRYNREHTWPNSLGFGSATGNLG
                     LPYAPYTDTHMLYLTDATWNADRGNKPYADCSSSCGERVTEANNGVGGGSGVYPGNSN
                     WVQSPDGNTGSFQVWGKRKGDMARAVLYMAIRYEGGVDPNTGQSEPDLELTNDRSKIV
                     ITSASPAYMGLLSTLLAWHAADPPDAAERDRNEVIQSFQGNRNPFIDHPEWATNALFT
                     STKPATCQLN"
     gene            28348..29142
                     /locus_tag="PK399_01365"
     CDS             28348..29142
                     /locus_tag="PK399_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPW11807.1"
                     /translation="MNLIDIGANLTHDSFDRDRDAVLQRARDAGVARMIITGASREHS
                     PKALTLAQAHPGALFATAGVHPHHAVEYTEECDAEMRALHAHPEVVAVGECGLDFFRD
                     FSPRPAQRKAFERQLQIAVETQKPLFLHQRDAHADFMAMMKNFDGKLGPAVVHCFTGS
                     REELFDYLDNDWHIGITGWLCDERRGQHLREIVGNIPAHRLMVETDAPYLLPRTLKPM
                     PKDRRNEPAFLPHIVEELARDRGEDVAIAAANSAATAAAFFRLPGA"
     gene            complement(29204..29674)
                     /locus_tag="PK399_01370"
     CDS             complement(29204..29674)
                     /locus_tag="PK399_01370"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HPW11808.1"
                     /translation="MKSLLICLSLFLLAGCAQQDHQRIGDLVVSVPWSREIPPTATVA
                     AGFVTIQNKGNRDDQLIRIESPTAKRVELHTMRHEDGVMRMRQLVEGVPIPAGQTVVL
                     APGGDHAMFIEPVRHATAGEQLQATLVFRDAGRLPVAFAVRGIAEQDEGGHGHH"
     gene            complement(29671..29835)
                     /locus_tag="PK399_01375"
     CDS             complement(29671..29835)
                     /locus_tag="PK399_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11809.1"
                     /translation="MNLPVLFLFAVSAWVMAWGVFLGAAWCTTRVADPHPITLLDDGI
                     PSLEPTETDR"
     gene            complement(29832..31115)
                     /locus_tag="PK399_01380"
     CDS             complement(29832..31115)
                     /locus_tag="PK399_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HPW11810.1"
                     /translation="MDIDTQLQGGRDMRRRAWRWHFLAALLVIPFVLWQSATGTLYLW
                     SEAWVDQRHPDLRFVEPAPRQVSLDVQLRAARDFQPGAKVANVLLPVDPARSTQVTFA
                     AASGLPLAVFVDPHRGVVLGSLEGAAWPVGWTRSLHGGWPLGDAGSWLLELGACWTIV
                     MVLSGLYLWWPRDGRGWRALLPRLRSGGWTFWRDLHACVAVWFSLLIVLFLFTALPWT
                     SFWGNKVLLPVQQALGQQGPRAAGFAPVFAGEAATEVGSLQQMLEQARSRGMAGDLMF
                     TMVDGPPGSAVSLRSVAANASDERYLLLDRANAQVLDDAGWSSFPLMAKTVATGVKLH
                     EADYFGRSGRWVNTAFALALAWLCVTGTMAWWRRKPARSLGVPPPSQRPWPWWLRVLA
                     LVGFLVLPLLALSAALLWLAENAWAHFANCRSKRA"
     gene            complement(31119..33257)
                     /locus_tag="PK399_01385"
     CDS             complement(31119..33257)
                     /locus_tag="PK399_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW11811.1"
                     /translation="MSLPSRTAMRSSCLSLAVLAALSCPVTAFAGDPPSDDADPQVAT
                     LGKVEVRAEKNGVEAERALTPGGVTVVDGETFEQRAVTNMADALRYVPGVLTQSGTGG
                     DAIFISSRGSNLDATNYDSNGIKLFQDGLPVTTADGNNHNRFLDPLAARHAVIARGAN
                     ALTYGASNLGGAIDFLSPTARNSPTMQLFLGGGSHGQVAARLTAGGVSGNLDGLVTLD
                     SLQRDGYRAHSRQDRMGLYANAGWQASDDLDLRVFATHVDSDEELAGPLTRAQFEQDP
                     YQAQSSAISGNFQLNVKTDRLAVKGTWSPDAGNRLEFGLSYEDQDLYHPIVDKVMVDF
                     DGPGPMPPVEVFSLLKNTDQRTWGGMLRYNARLGDHDVLAGLNVANTHESGGLFRNDG
                     GQRNGLRTIVDNRSDSMEVFVVDRWTIAPAWTLVYGAQGVFTGRDVRNTDVASGVLRH
                     PKDDYASFNPRIGVIRALTPASEAFASVSRLYEAPTTFELQDDVRGGDATLDAMHGTV
                     AEIGVRGNTTGPTDAIRWQWDASLYYARIHDEILSIDNVAAPGTSLSTNVDRTVHAGI
                     EALFGAAIPLGGGYRIEPLLSATYNAFSFDDDAVYADNRLPAAPRHAIRGEVMVRNDK
                     GFFAGPTFDVVGARYADFSNTYRVAAYELLGLRVGFERERWEVFGEVRNLLDREYVGV
                     FSVLDRASAGSAILQAGEPRSVYAGLRLRF"
     gene            complement(33318..33743)
                     /locus_tag="PK399_01390"
     CDS             complement(33318..33743)
                     /locus_tag="PK399_01390"
                     /inference="COORDINATES: protein motif:HMM:NF022610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HPW11812.1"
                     /translation="MIRSTTFRNLMARLALAAMLLMALAPTISRSLATLPDANGAVLR
                     ALQMCTSAGLQTRLIAPLLGSDAAGFGGDEHPAPHPHEPGDACGYCSLVTPLPLLLLL
                     LCGLLALPPIAPLFRRRTHAPRTPRNLRGLGAQGPPLTL"
     gene            complement(33773..>34072)
                     /locus_tag="PK399_01395"
     CDS             complement(33773..>34072)
                     /locus_tag="PK399_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW11813.1"
                     /translation="FINAVYAKKFDDVAIVPTFILTPLTYLGGVFYSVKLLPGWAEAA
                     THANPIFYMVNAFRYGLLGVSDVSMWTAYGLMLGFVGVLGALALWLLKRGVGLRS"
BASE COUNT         5357 a        11838 c        11544 g         5333 t
ORIGIN      
        1 ccggcaccgt caaccaatca ctccccggaa aagttgacgg ctgcctggcc gctcccctcc
       61 cctggctgtt cgcactgcgc tgcatggcct gcggcgagcc gggccaagaa ggccgcgacc
      121 tgtgcggcgc ctgccacgcc gacctgccct ggcaggggcc ggcctgtctg cgatgcgccc
      181 tgccgattcc actggccggc acctgcgggc attgcctgca gtcgtcgccg cccttgggcg
      241 aggtgcacgc ggtgttcgac tacgccttcc cgctggaccg gctgctgcct cggctcaagt
      301 tccatcgcga tttcgccgcc gggcgggtgc tggcgcagtg catggcggac aggctggcgg
      361 cgctgccgcg tcccggggcc atggtcccga tcccgctgca ccgcgcacgc ctgcgccacc
      421 gtggctatga ccaggcgctg gagttggccc gcccgctggc gcgcgcgctg ggcatcccgc
      481 tggtcgatcg cgcgctggga cggcgcaaga ccacgcgtgc gcaatcgcga ctggatgccg
      541 acgcccgccg gcgcaacctg cgcgacgcct tccatgtcga tgccggcatc ccgttaccgc
      601 cccacgtggt gcttgtggac gatgtgatga ccaccggtgc gaccttgcat gccgcggcag
      661 gggtactgca tcgcgccggc gtggcgcgcg tggatgcctg ggtctgtgcg cgcgtcgcct
      721 gatggctgct caggcggcga ctgcggtgcc gcgcatcgcg aaatcgacgg cgatgccgac
      781 gaataccgcc atgcccaccc agtggttgtg caggaacgcg cggaagcagg ccgcgcgctc
      841 gcggccgcgg gcgatgccga actggtacgc gaccaacagc agggcaaccc ccagcccgcc
      901 ccagtaatac acgcccaggc cggcatcgcg accgaccagc gccagcgccg cgaacatgca
      961 ggtgtagagc acgccctggg cgacgaggtc catgtcgccg aacaggatgg ccgtggattt
     1021 tgcgcccatg cgcaggtcgt cgtcgcggtc gaccatcgcg taccaggtgt cgtaggcggt
     1081 ggcccagaag atattcgcca cgtacagcac ccacgccacc ggcgggacct cgccggtcag
     1141 ggcagcgaag cccatcggga tgccccagcc gaaggccatg cccaggtaga cctgcggcag
     1201 gtaggtgtaa cgcttcaggt agggatagct cgcggccagc agcacgccga ccacgctcat
     1261 ccagaccgtc aggcgattga gcgtcagcac cagcgcgaag gcgaccagca tcaacaccgc
     1321 gaacaccgcc agcgcctcct ggcccgacac cgccccggtc gccagcggtc gctgcttcgt
     1381 gcgctcgacg ttcggatcca gccagcggtc ggcgtagtcg ttgatcacgc aaccggcgga
     1441 acgggtcagc cagacgccca gcgagaacac cagcagcggc cacagcggcg gcatgccctt
     1501 cgccgcgatc cacagtcccc accaggtcgg ccacagcaac agcaaccagc cgatcgggcg
     1561 atcgccgcga accagcgccc agtactggcg caggcgttct cgccagagcg gttcgatgcg
     1621 aggctgctcg gcgaagtcgc ggtcgttcgg catcgaacct gcggagaatg gcgcgcccgg
     1681 aggggtgagc cgtcgcgctt gcaagccttc tgcttgcgcg gcggcgaacg cccgagcgct
     1741 ggacgcgagg gcaggggaat cccgacctcg ttgcaacatg gagcatggcg cgcccggagg
     1801 ggtaagccag cgcggctgca agccatcggc ttgcgcgctg gcgaacgccc gagcgccatg
     1861 cgcgagggca ggggaatccc gacctcgatg gcacctagtg tatggcgcgc ccggagggat
     1921 tcgaaccccc gaccaatggc ttcggaagcc actactctat ccggctgagc tacgggcgca
     1981 tgacgtgcgg cgaccgccgc ggacgcgcat tctcgcatgc ttgcgccgga ccgtcatcgc
     2041 caaacgaagc ggcccgcttg cgcgggccgt ccgttcacgc gatgtccctg cgggcggtca
     2101 gttgaccttg accgcgctcc acgccgcggc cacggcgttg tgctgggtgg agccagcgcc
     2161 gtacaaatcg gccgcggcac tcagcgtcgc cgcgcgggcc tgggcgtagt tggtgcccga
     2221 ggtcatgtac acggtcagtg cgcggtacca gatcttcccg gcggcggtgc gaccgatgcc
     2281 gaccaggctg gtgttgccgt tgcagaccag cgacgacggg gtcaggttgg cccaggtccc
     2341 ggcgccgaag ccggccggca ccaccgcacc ttccgcaagc aggtagaaga agctgttggc
     2401 gatgccggag ccgccatgca cctcgaaggt cggggtggtc gccgggtcgt agcaatgctg
     2461 cgagtagttg tccaaggtcg gcttgaacat gtagcggatc gcctgcgaca tgttggcgtt
     2521 gttcttgaac agctcctcgc ccacgacgta gtcgcccgga tcgttggcgt tgttggcgta
     2581 gtactcgacc atggtgccga agatgtcgga ggtcgcttcg ttcagggaac cggactcgcc
     2641 gctgtagatc aggttcgcgg tgcggctggt gacgccatgc gacatctcgt ggccggcgat
     2701 atccagtgcg accagcggca ggatcgtcgc gccattgtcg ccgtcgccga aggtcatgca
     2761 gaagcagccg tcgctccaga acgcattctg gtagttgcgg ccgtagtgca cgcgggtcag
     2821 cgcgccaacg ccgtcattgg cgatgcccgt gcggccgtgc atgttcttgt agtaatccca
     2881 ggtggtcgcc acgccgtaat gggcgtcggc cgccacggtc tgcgcgttgc tgaccaggaa
     2941 atcaccccag acgttggtgg tgccggtgaa caccgacccc aggccggcgg tcttcagcga
     3001 catgttgttg gtggcgccgc cgccgcgggt catgtcgacc agctggtagg tgctgccgca
     3061 cttggtggtg ttgaggacca cggtgccctc ggtcaggctc ttgccggtgc cgaccgccgc
     3121 ggtggcgccg gtgcagccgc tgccgcccgg accaggcttg gcggtgtgga cggtgtccca
     3181 ctggtcgagg atgcgaccgc tgctggcatc gacgaagtaa tgcatttcgg tcggggtctg
     3241 gtcggccttg atgccgttga agacgacttc atgggcaagc acggggctgg cgccgcgggc
     3301 gtacaccacc atgcgcgagg tcggcgcgcc gaagaacttg ctgccgaagc gggcgcccgc
     3361 ctcgacgatc gcttcgtcgc tgccgaactg ggcgaccagg ttcgggcgcg ccgcgctctt
     3421 caggctctgg ctgacgttgc gcagctggcc attccgggaa tgcatgacga agtcgccgcc
     3481 gatcaccggc aggccgcggt agctgcgctc gaaacgcacg tgctcggtgc catcggcatc
     3541 gacgatggcg tcgcgcgcgg cgaacacgtc gcccttcgca ccacggatga tgccggtgcc
     3601 gttcgcaccg agcagcacat tggcgcggct gatggcggcg gacttcaggt tgccggcagc
     3661 ctcggcgggg atcgccgcaa gggcgaggcc ggaagcgatg gcgagactca acaaactcat
     3721 ctgggctttc atggcgcgtt ctccgtggtg atcaggaccg gccgttaccg tgccggggaa
     3781 tggatggtgc aggccgggtg ccggcgacgc ggcgtgtccc gggaacggcg gcaggcgcgc
     3841 ccagcggcgt tggcgcctgc aggatcgcgt cttcgaaata ggacacgcgc agctgcaagc
     3901 cgcgcggcga gtagccgatg aaggtggcat cgaagccggg ctcgtgcgcg cgcttgtagg
     3961 ctgcggcgat cttggcgttc tcgacttcca gcaggatgcc cagcgactcg ggcttgtcca
     4021 ggccggcgtt gtagtcctgc aggatctgtt tcgcgcggcg gtagaacgcc agttcctggt
     4081 gctgtccgcg caacgccctg gcgaagaact ggcggtagta gtacatcggc tggcgcaggc
     4141 gcggcagcac cttgaccacc gccgtggcca ccccgcacaa cgcgccgtcg gcatcgcgtg
     4201 cgatgcagac cgcctgttcg gtgcgacggg ccgcggccgc ctcgttctgg atcgcgttct
     4261 ggtcgcgcca gaaggccacc agctcggccg ccagttcggg cgacagggcc ttccagaccg
     4321 ggacgatctc gatcttcact tggcggcggc cttgccggct tcgggcgcca gcgcctgtac
     4381 cagtttcacg tactcgccga actcgagctt gccgtcgcgg ttcgcgtcgt agaccgacat
     4441 ctgcggcgcg ttcttgccgg cctgcttgac cagcaccagg ctgccgtact cggccgggtc
     4501 gatcgcattg ttcttgttcg cgtcgaccgt gccgaactgg tggcgcagcc gtgcctgcat
     4561 ctccgcgacg cgctggacct gttgccagcc ggtgcggaac tcgaccagcg acaggctgcc
     4621 gttgccatcc ttgtcccagg ccttgaacgc ttccgcggcc gggtcctggg ccgggcgcgc
     4681 cggtgtcgcc ggcttcgccg ccgacgactg cgcagcgacc ggttgcgcac cgagcaggac
     4741 gagcccgcac gccattgcca tcatcgtgcc gcggatcgtg ttcatgcagc gctcgcctcc
     4801 gcggcaggca ggtcggactc gaaggtgatc aggtcgcgga tctcggcggt gcgcaggccg
     4861 atcatcatcg ggttcttcac gttcggcagg aacgcgatgt cgaagatctc gctgatgtcg
     4921 ccctcgaagc ggatccagtt cagcacgtcg ccattggcca gcgagatgat ctgcaccccg
     4981 caccacggct cggcgtcgcg tttcttcaat tcgccgtcga gttgcaggcc ttcgaagcgc
     5041 tggttgcgcg gcttcgacag gccgaccgcg gcgacgttgc cgatgatcga cagcccgcgc
     5101 gcgaagcccg ggaccgacgc atgcggcacg aacgccttct tctcgaaatc gacccagccc
     5161 aggtggccgg tgccggcgtt gagcacccac agcctgccgt tgtgccagcg cggcgagtgc
     5221 ggcatcgaca ggccttcgca gacgatctcg tcggtctgca cgtcgatcac cacgccgccg
     5281 tcctggcggc ggtcgcgcca gccatcgacg ctgtcgctgc ggcagaccgc ggtcacgtac
     5341 ttgggctcgc catcgaccat cgccaggccg ttcaggtggc acctgtcctc cggcgccagc
     5401 ttgctgatga acgtcggctt ccagatcgcc ttgaagctgt gggtctggct gagctcggcc
     5461 aggcaggagt acttcgtgtt gacgaagacc accttgccgt tcttgcggat gccgagttcg
     5521 tggatgtcca ggtcgccgat ggtctgcgcg ttgcgcggga cgtagcactt gtcgaactgg
     5581 ccgtggatca cctcgttcgg gcgcagcacg ttctcgaagc gccacagctg gtacagcccg
     5641 cccaggtaga tccgctgcgc attgccgacc acgcccatgg cgcgctcgaa gatgcgttcg
     5701 aagaacgaga cgcgaccctt ctggtcgctg ccgaccaggt agatgcgacc ggtctggtag
     5761 gaggtgatcg ccagcgagag ccgatgctgg gccagccaac cggcgagccc gcgcgaacag
     5821 gacttctcga ccttgagctt ggtgatgtcg agcggcttct tctgctcgcc ggcggccggc
     5881 gccggcgcat cggccgagac cgtgtccagg cccgtgtcca gttccggctt gtcctggtgg
     5941 ccctggacct gcggttcttc ctgcgtgttc tcgttcgtca cgtgcatccc gactgcgtta
     6001 ctgcaagacg acgggcccgc tgccgggccc gccccatccc acgattacca ggcgaagcgg
     6061 atgttggcct ggccgctcac cgtgctcttg tacgcaccgc catcggccca gttggcgccg
     6121 atggtgaagc cccagccgcc ggactgcacc ccaaggccca gttccgccat cgcgctggtg
     6181 ccctcggtgc tggtgctgcc gaagaagttg gtgcccacgg tgtactccat ctcgccgtcg
     6241 aactcgcgga tcgcgttgat cgagccgtac ggcgtccagc gcaggttgcc ggcggtgaag
     6301 gtcttgttga attccacgcc cacgcggccg cgttccgagg taccgccgtg gccttcgaag
     6361 gtcgcgcggt cgaaggcgat cgcgcggatg tcttcgacgt ccgtgcgggt gtactgcacc
     6421 tgcgggacga tgttgaagcc gcccaccgcc cactcgtaac ccgcttccag gctcttcgac
     6481 gtcgtgtgcg cgcggctctg caacaggccc gcgatggagg tcgattccac gtccacggcc
     6541 atctggcgat gggtcaggtc gacgtagaag ccctgcggca cgtaccaggt cgcgtacgca
     6601 ccccaggtca tgccgtccat gcggttctgg cccacgccgg caccggtcag gcgctggcgg
     6661 ctgtccgccg tgcccagcac cacgcctgca tggaagttcc cgaacaggtt ggcgttgacg
     6721 ccgacttcgc ggccccagct gctcaggtcg taggcgaagt tgccgccctg gccgaagttc
     6781 gccgccacgt gcgcctggtc gacgtcgccc tcgctggtgt agacgcggac gaacgcgctc
     6841 atcaccttgc cggcgtcgcc gtagggattc acgcccaggc gctggcggaa ggtgccgacc
     6901 tggctgttga ggaagtcggc ggcacccgac ccgatcgagg ccgccagcga gccgctgtca
     6961 ctcaggccgg ccacgtcgac gttgatcgag aacaggtcgg ccgcggtgtt ggcggtgttg
     7021 atctggctgg tgaccgtgag gtcgaggtcg aggaagttcg ccgggctgta gccgaagatc
     7081 cggccggcca cgaagttcga cgccgtcgac gtgccgctga cccgcccgaa caccgcggag
     7141 gtgtgggccg tggtggggag gcccggggcc gccacgttga ccgtctgcac cgcgttggcg
     7201 gccacgttgc cggtcaccac cagctggtcg accgtgctgt tgagcatgtt caggtcgagg
     7261 ttgagcgcgc cggtgccggc cagcgtgccg accacggtca gcctgtcgtc ggtcgcgccg
     7321 tcgagcaggt cgatcgtgtt gacgttggtc agggtgccgg ccgcgcccgc gtccacggtg
     7381 ctgttgccca gcgccttgag caggctgatg ttggtgaagg cgttggtgcc ggcgcccatc
     7441 gtcaccgtgc cgccggtcag gcggaacgtg ccgaggttgg tgatggtgtc gttgccgtct
     7501 tccatcgcca ccgtgccgcc gaccaggtcg accaggccac ccgcggcatt gctgagggtg
     7561 tcgttgccat cgccgaagct gatcgtgccg ccggtgccgg tgcgcaccgt gccggcattg
     7621 gccagggtgt cgttgccggc gccgaaggtg atcgcgccgc cgaggatgtc gaccaggcca
     7681 cccgccaggt tgttgagggt gtcgttgccg tcgccgaagt cggtgctgtg ggtgccgccg
     7741 gtgctccaca cgccgccgct gcggttgttg aacacgtcgt tgccgccgta cagcgccagt
     7801 gcgccggtga tgtggccgtt gttgttgatg gtgtcgacgg cgtcgctgcc gagcaccgcg
     7861 aacgcgtagc catcggtacc ggtgacgctg atcatgccgg ttgccgcgtt gttgagcgtg
     7921 ctggttccgt acatcgattc catcagcacg gcggcggcat gcccgccggc atcggcgctg
     7981 gccgacaccg cacccgcgtt gttgacgacg acgttgccgt aggcgttggc gaggatgccg
     8041 taggcataac tcggggcggc cgcatggatg tgcccggtgg cgctgttgcc caccgtgaca
     8101 tcgcccaggt acgactgcgc cgcgatgccg taggcaccgt agtagccggt ggcctcgatc
     8161 gtgccgctgt tggtcaccgt ggcgtcgccg gccaccgagt aagcggtgat gccgatcgcg
     8221 gtgccgtatt cggaacttgc ggcgatctcg ccggagttgc cgaccgaggc attgcccagc
     8281 ccgctgtagg cctggatgcc cacgccgccg cccaggacgc cctcgaccga gatgtcgccg
     8341 gcgttggtga ccgagacatt gccgtattcc gagcgaccct cgatgccgac cgtcaccagc
     8401 agcgaatacg cctcgatgtc gctggtcgcc gtggtggtga cggtggtgcc gtagagcgaa
     8461 cgcgccgcga tgccggtggc ggagtagatg ccgaacccgt agatgctgcc ggcgttggcg
     8521 acctgggcat attcgccgat cgccaggatg ccgtacgcgt cgtcgccgat gtagccgttc
     8581 acgccttcga cgctggccac gatgtcgccg ccgttgccga ccgaggcggt gccatagtcg
     8641 gcgcgggcga agatgccgac cgccacgttg ccgccggtga tcgcctcgat cgaaccggtg
     8701 ttggtgacgc cgacgtcgcc ctcgccggag cggacgaaca cgccggccga ctggccatag
     8761 gcgtaggtcg cgatgctgcc gtcgttgacc acgtgcacgt cgccctgcgc cgcgtagccg
     8821 aacacgcccc aggcgcggcc gatgtaggag taggcataaa tgtcgccgcc atcggcgttg
     8881 gtgaccgaga cgtcgccggc gccggcggtg gcatacacgc cgaacgcgta cttctggcca
     8941 tccgcgtgca ccgagccgct gttctcgacg gtggtgtcgc cgtagccgcg cgcgtcgatg
     9001 ccggttgccc agtagctgct caccgcggcg atgtcgccgc tgttgtccac gttcgcattg
     9061 ccgttgccgg cgaacgccac gatgccgctc gccgcgtagt acagggacga ggtgttctcg
     9121 acgacgatgt cgccgctgtt ggtgacgttg gcatcgccgg cgaacgacag tgccgtcagg
     9181 ccattggcca cgccgcccga gtacacgtcg accaggccgc tgttgtcgat cgcgccgtcg
     9241 ccgtccgccg acaccacgat gccgtacgcg ccatagccgt acttctggtt ctgcgagacg
     9301 tagatgttgc cgctgttgtc cacgctggcg gtgccgccca ggccgccgga cgtcgcggcg
     9361 atgccgctgg cgccgaagat gccgtcggcg ctgatgtcgc cgctgctgct caccgcgacg
     9421 tgcgcgcctt cgccattgct ggtcgcattg atgccggtcg cgacctgggt gccgctgtag
     9481 acgtagtacg cggtgtaggt gtcggggttg tagtaggccg cggactgcag gccctcgccg
     9541 gcgacgatgt cgccgctatt ggtgacgctg atgtcgccgc ccgactgcac ctcgatgccg
     9601 gtcacgtagc cgccctgcac ctcgatgttg ccgctgttgc cgaccgtcgc gccgccttcg
     9661 ccggccgtcg catagatgcc gaacgcctgc gcgcccgggg aattggcgat ggtcccgccc
     9721 gcgtagtact cgaaggaata ggcatagacc tggtacaggc cgggcgccgt ggacgtgatg
     9781 tcgccgctgt tgtccaccac gaccgaatcg tcgccctgcg cctcgatgcc ggccgcccag
     9841 gtgtagccat agacctcgac cgcgccggaa ttggcgacat cgacgttggc gccggaggcg
     9901 aagatgccgt cggccagggc gtagtacgag atcgcggtga tgtcgccgct gttgtcgatg
     9961 ctgacgtcgc cgccgaccga gtagccgtag atgccgatcg aattgccgta cagcgagccc
    10021 gcgtagatgt cgccgctgtt ggcgacgtcg accatgccgc cgtcgtaggc cgccgcattg
    10081 atgccgcgcg cgcccagcac gatgccgacc gcgtcgatgt cgctggcgtt gtcgaccgag
    10141 acgtcgccgt agaacgagcg ggcgtcgatg ccgaggcctt cgcccaccgc gatcgcgctg
    10201 atgctgccgc cggtggtggt gaccgtggtg ccgtagtagg acgtcgcgat gatgccggcc
    10261 gccgcgtaga agccgctggc ctcgatgtcg ccgctgttgc tgacgctgga ataggcgccg
    10321 cgcgccagga tgccgtaggc ctggccgtag tacgagtagg cggagatcgt gttgctgttg
    10381 tcgaccgtgg cggtgccgta gtcggcgcgg gcgaacacgc ccagtgccgc gccgtcgtag
    10441 ctgtaggcat cgatcacgcc gctgttcgac acgttcacgt cgccggcata ggccaggccg
    10501 aagatgccga ccgactggcc ggtgaccgcg gtggtgatgg agccgctgct gtcgaccgtc
    10561 acgtcgccgt tgccgctgat gccgaggatg ccgaacgcgg tgccgccgga tgcgccgtag
    10621 gtggcgtaga tcgagccttc gttggagacc tcgacgtcgc cgttgccggc ctgcgcgcgg
    10681 atgccgtagg cgcgccacgc atcgaccgag atgtcgccgc tgttgctggc agtcgcgcct
    10741 tccaggccgc tgacgtcgat gcccgagctg atgtacttgc tggtgacgtc gatgttgccg
    10801 ctgttgccgg cgtaagcgct gccattgccg gcgaacgcca cgatgccgtt cgcgccgtag
    10861 tacaggaacg catcggtgct gacggtgatg tcgccggcgt tggtcacgct ggcgtcgccc
    10921 gcgaaagaca gcgccaccgc gccgttcgcg acgccggccg agtccaccgt gatcgaggca
    10981 ccggcctggt tgtcgatgct ggcgtcgcca tccgcggaga ccacgatgcc gtaggcgcca
    11041 tagccgtact tgttgtactg cgacacgtag atgttgccgc tgttctccac gctggccgag
    11101 ccgcccaggc cgccggaggt ggcggcgatg ccggtcgcgc cgtagtagcc ctgcgaggag
    11161 atgtcgccgc tgttggtcac gccgaccgcc gcgccctcgc cgttgctgat ggcgttgatg
    11221 ccggtgccga ccaggctgcc atacaggtag gtgtagccgc tgtagtagtc gtagaccgcg
    11281 gtcagcgagc cgccgacgat gtcgccgctg ttggtgacct gcaggtcgcc ggaggactgc
    11341 gcctcgatgc cggtgccgct gtagccctgc gcctcgatgt cgccgctgtt gtcgacggtg
    11401 acgtccgcgc cggtcacgta gatgccgaag gcggtgccgg catccaggac ttccgcatag
    11461 atgtcgccgc tgttgtcgac atggaccgtg ccggtgccct gcgcctcgat gccggcggcc
    11521 caggaatagc cgtacacgtc gatcgcgccg gaattggtca cgtcgacgtt cgcgcccgag
    11581 gcgaagatgc cgtcggccac gtagtaggcg gacaacgcga agatgtcggc ggcgttgtcg
    11641 atgctgacgt cgccgatggt cgcgtagccg tagatgccga tcgcattgcc gccgagcgac
    11701 gccgccagca ggtccgcgcc gacgtggttg gtgatgtcga cgttgccttc gctgctgtag
    11761 ccgctgatcg cggtggcgct gtagtagatg ttgacgtcat cgatggggtc ggcgttgtcg
    11821 atgaccacgt cgccgatggc gtactcgctg atcagggtcg ggtgcagctc cggcggcacc
    11881 tccatccaga tcggcggggc gacggtcgca aggtcgtgga ccggcgcgaa atcgagcgtg
    11941 acgtgctgga cctgcaggac ctgcgcctgc ggagccttgg ggccggcggc gaaggcgcca
    12001 ccgctggcgc cgagcgccag ggccatcgcc gaaacggcca gcgcggagcg cacggcacgg
    12061 gtcatggggg ccagcttgat ggtgcggctg cgcttcgtct tcgccgaccc gctcacgttc
    12121 cggatattgc tcatttgacc cccaaggtcc atagctgatt tgtatgtcgc ggcttgccgc
    12181 agaagcgaaa aatgccttgc cgatcacggt gtccggcacg tttcgcctgc gcgggattgc
    12241 ctagcggacg actggtccaa gcacgcctgt tccccggcgc acaaggtttc cctcaacctc
    12301 tagttatggc catcgcgcct tttcgtgtca atagaaactt tgacctagga cacgtttggc
    12361 cttgcctgaa tgcgggccca tcgaagcggg ggcgcctcgg tgaaaaggcg ctagcggcga
    12421 cccgccagct gcaccggcga ccgatgcccc ggcatgccgt tggcgaccac gcaccggcaa
    12481 cctcgcgctg cacggcctgt tctgggataa tccgtggttt ggtggcttcg gccggacccc
    12541 cactcgcagg tggatcgtgc gtaattacga ccttgaattc ctcaagcgct tctcgatggt
    12601 gctcgccttc ctggcgctgg tcacccttgg cctgatcctg ttcgcgcacc acttgaacag
    12661 caaggtcgtg cacgaagtcg acccggccac ccaccagcgc ctgctcgcgc gcatcgcccc
    12721 caccggcgac gtgtacgccg gcgcgaccgg tgccgccgcc caggccgccg ccgccgccgc
    12781 cgcacaggcg tcgctgctgg ccaacatccc gttcgatggc cgcaccgacg gtgcggagat
    12841 cttcaataac agcacctgca cgggctgcca caccgccggg gtcggcggcg cacccaagct
    12901 ggaaccggcc gggatcggcg cgcgcctggc cgagcaaggc ctcgataccc tggtcagcaa
    12961 ggcgatcagc ggtttccagg gcagcgccgg ggtgatgccg gccaagggcg gcaacccggc
    13021 cctgaccgac gaacagatca aggccaccgt ggaatggatg gtggcgcaga gcaagtaagc
    13081 gccgctttcg tcgcatggac gccgccttcg ggcggcgttc gcgtttccgg gcgggcgttc
    13141 gcgggccgtc ttcggcgttt cacccatcct ccggcatcat ggccgcatga gcgaatcccc
    13201 cgccgccccc gccagcgccg acctgcccct ggccatcgcc ggacccgccg gccgcatcga
    13261 ggccgccttt gacctggccg aagccgacgc cacgccgcag ccggtgctgg cgatcgtctg
    13321 ccacccgctg cccaccgagg gcggcagcat gcacaacaag gtggtgacga tgaccgcccg
    13381 cgcgctgcgc gagtccggca tcgccaccct gcgcttcaac ttccgcgggg tcggccagtc
    13441 cgagggccgt ttcgacgatg gccgcggcga actggacgac ctgcgcgccg tggcggcctg
    13501 ggtgcgcgtc gaacatccgg acaaggcgct gtggttggcc ggcttcagct tcggcgcgtg
    13561 ggtggcgttg cgcggggccg tcgagttggg ggccgccgcg ttgatctcga tcgccccgcc
    13621 ggtcgggcgc ggctggtcgt tcgatggggt ggcgatgcct gccgtcccgt ggctactgat
    13681 ccagggcgaa gccgacgaaa tcgtggacgc cggcgcggtg ttcgactggg tcgccacgct
    13741 gccgcgcccg ccggtcgtgg tgaagatgcc ggacaccagc cacttcttcc atcgcaagct
    13801 gatcgacctg cgtggcgcgg tgaggcacgg cgtacgcgac tggttgccgg tgccgccggc
    13861 cgcatgagcg atccgctgct cccctcgcgg cgctacgccg acggcgtcgc gcgcggcgac
    13921 tggcagtacg acccggcgca gcaaccggcc ttgctcgcgc tggatcgcgt ccatgccgcg
    13981 ctgcaggcgc cggtgcccac ccgtggcgtg ttccagcggc tgttcggcaa cccgccgccg
    14041 cctgccccac ggggcctcta cctgtggggc ggggtggggc gcggcaagac cttcctgatc
    14101 gatctcttct tcgccggcct gccattcccg gaaaagcgcc gaacgcattt ccaccgtttc
    14161 atgcgcgacg tccaccagcg cctgcgcgac cacgccggcg aacgcgatcc gctggtggcg
    14221 atcgcgcgcg aatggcggcg ctcggtcagg gtgctggtgc tggacgagtt cttcgtcagc
    14281 gacatcggcg acgccatgct gcttgggcgc gtgctggatc gcatgttcgc cgagggcatc
    14341 gtgctggtga ccagctcgaa tgccgcgccg accgacctct acaaggacgg cctgcagcgc
    14401 gcgcgcttcc tgccggcgat cgcgctgctg caggaacatt gcgccgtggt cgaactggtc
    14461 agcgacaccg actaccggct gcgcgcgctg acccgttcgc ccgtgtaccg ggcgccgctg
    14521 gatgccggtt cggatgcctg gttgcagcag cgctggcgcg agctgggcgg cgacgacggg
    14581 cacgtggatg ccggcatcat catcgacggt cggcgcatcc cggtgcgcgc gcgcagcaag
    14641 ggcctgtgct ggttcgactt cgccgcactg tgcgaaggcc cgcgcggcaa cgccgactac
    14701 atcgaggtcg cccgcgagtt ccacacggtg ctgctcggcg gcatcccgcg cctggacggc
    14761 aaccgcgacg acgcggcgcg gcgcttcgtg catctggtgg acgagctgta cgaccgccac
    14821 gtcaacctgg tgtgcaccgc cgaggccgcg ccgatggacc tgtacgccgg cgaacgcctg
    14881 gtcgccgcct tcgagcgcac cgcctcgcgg ctggtggaga tgcagtccac ggcttatctc
    14941 gcccatgaac acggcggctg aagccggcat gcccgcttgc gaagtcccct agaatcccac
    15001 cccttgccaa ggtccagagg ccgcccatga cgtccatccc ccgcatcgcc ggatccgccc
    15061 tgctcctgct ggccctggcc gcctgcaacc gccagggccc cgacgtcccg aaaccggtcg
    15121 acgacaacaa ggagttccgg gaccaggccg acggcatcga gtcggtggtg gtgtcgcccg
    15181 atgcggccaa ggtcggcagc gaggtcgatg gcgccggaga catcgccaac gaagtgcgca
    15241 ccttcgacgc cgggcagaag gtctacatct cggtgcccgc gaagttcggg cgcaagatcg
    15301 gcgacaagct ggagatcttc tggttccacg acgacaaccg ctcgcgccac gaggagcaga
    15361 agcagattgc cggcccgttc acggtgttcg agttcgcacc gaccgatgtc ggcgcctaca
    15421 acgtcgaaat cgccgccaat ggacggccga tcgcgctggt ccagttcgaa gtgaagtaac
    15481 ggcggcgcag cgcgcgatgg gcgacatcga ccactccccg ctcggccgcg aggtcgccta
    15541 cccgcgccac tacgacgcca gcctgctgta cccgatcccg cgcgcgcttg gccgcgcggc
    15601 gatcggggtg ggcgcgccgc tgcccttcat cggcgttgat cgctggaatg cctacgagtt
    15661 gtcgtggctc gaccccggcg gcaagccacg ggtcgccacc gccaccctgc gggtgccggc
    15721 ggattcgccg cacctggtcg aatccaagtc gctcaagctg tatctcaacg cgttcaacgc
    15781 cacccgcttc gccgatgccg aggcggtgcg cgcccggatc gaggcagatc tcacccaggc
    15841 cgcgggcgcg ccggtggcga tggccttcgg cctgccgccg ttcgaagacg cagtcgatgc
    15901 cgagagcatc gacgccctcg acatcgccat cgatgcctac gacgcgccgg atgccgcgct
    15961 gctggtcgcg gatgccggcg acgtggtcga cgaggcgctc acaagcgcct tgctgaagtc
    16021 caattgcccg gtgaccggac agcccgactg ggccgatgtc accctgcgtt accgtggtcc
    16081 gcgcatcgac cgcgccagcc tgctgcgcta cctggtcggg ttccgcgacc atgccgagtt
    16141 ccacgaacaa tgcgtcgagc gcatcttcgt ggacgtgctg gcgcgctgca ggccgcactg
    16201 gctgtcggtg gaggcgcgtt acacccgccg cggcgggctg gacatcaatc catggcgagc
    16261 gaccgccggg atcgacgcac cgaccgcgct gcgcaccctg cgccagtaga ttccgccacc
    16321 accccggtca ccttgccgac atgtttcctt aacggcgtgc gtgtcagcgt gcgcctgccc
    16381 gaatccggga aggagcaacg catgagcaac gacaagacac ccgatttttc caatgtgcag
    16441 ggcggcgtgc agagtaccga gcagctcaag cccgacttct ccaatgtcga atcgaaggtg
    16501 acctcgaccg aggcgatcct gggaggcgaa ggcggtggcg gcagcaccgg cgaacgcacc
    16561 tacaccgtcg agaagggcga caacctctcc accatcgcca agcggttcta cggccggcag
    16621 gactggaagc gcatctacga cgccaaccgc gaccagttgg acgacccgga ccggatcaag
    16681 cccggccagg tcctgaagat cccacgggac gatcagcagg actgaccgcg ctgcgctcgc
    16741 tttccccacc tccagacacg caaggactcc ccatgaagac caatcgcctc cagatggcca
    16801 tgctcgccgc gatgatcggc agcgccgcac tcgtcggctg caagaagaag gaagagccgg
    16861 cagcgctgcc gcccgtcgcg accgaacccg cgccgaccgc gcctgcgccc gccccgatgc
    16921 cggccactgc gtccgtcagc gcggtcgacc ttggcaatgc cgtgggcgcc gacatgcgcg
    16981 tgaccgcccc gatgtccagc ttcgcgccga aggacaccat ctacgccgcg gtcagcaccg
    17041 ccaccagcga tccggccgcc accgtgcccg gcaagctggg cgtgaagtgg acccacgtcg
    17101 acagcaacca gaccgtgcac gaggaaagcc gcgacatcgg cttcgccggc agcggcgtga
    17161 ccgacttcca gatcagcaag ccggatggct ggccgaccgg caagtacaag ctcgaagtct
    17221 cgctggacgg cagcgtggtg cagacccgcg agttcgaggt caagtgacgt aagcgcgccc
    17281 atgcgaaacg cgcgtgggcc tttccacgca cgtcatcccc gcgcaggcgg cgatcccgcg
    17341 tttgggcgtt cgcgaactcc catcgaaaag aagggcccgg tgcaaaccgg gcccttcttt
    17401 tttcgatgaa cactcaaggc gagggtgtcg cggccctgcg gtagatcccg tcgaacgcga
    17461 ccgtccacgt cttgccgtca tcggtcgagg tttcccacag ctggcgcacg ctgccgtcgc
    17521 cattcggcgt ccaggtgatg cgctcgtgct gggtcagccc ggtctgcgcg ttcgccacgt
    17581 cctgggtccc gcgcagcacc atcgcgccat cgaccagccc gcccttcagg tgcaggcggt
    17641 tgccgccgtt gtcgacccag aactgttccc actggcgggt cgccgtgttg tagatgttga
    17701 agctctggcc attgctgccg ccggcgccgc tccagtgctc gtggatgacg caacggtcga
    17761 gcgtggcttc gatccggctg ctgccgaccg gcttgccgcc ggggtcgctg accgtccatt
    17821 cgccgatcca gaaatcgaac tgtcgatgca tggcctggct gcagggcttg gggcgattgg
    17881 cgggcggctg ctgggcggtc gcctgtccgg gtaggccggc aagcgccatc gccagcaggc
    17941 caggcagcag gatggcacga cgcatcggca tgttcccggt ccccgaaggt gatggcgtgg
    18001 ccaccatgca cccatcgcca ggccgatgcg cactgccgga ggtcatgccc gcgcacatcg
    18061 gccgcgcccg tctttgcccg cggcggcgtg aagcccgaca atggcggtct tccgccgtga
    18121 ccgcgagccg ccatgaccgc ccagatcctg tacacgctca ccgacgaggc cccgttcctc
    18181 gccaccgcca gcttcctgcc gatcgtgcag gcctatgcga agaccgccgg cgtggacgtg
    18241 gcgacccgcg acatctcgct cgccgcgcgg atcgtggcgc agttcaacga cctgctgccg
    18301 gaagaccagc gcaacagcga cgacctgcgc gaactcggcg aactggcgaa gacgccggaa
    18361 gccaacatca tcaagctgcc gaacatcagc gcctcggtcc cgcagctgaa ggcggcgatc
    18421 aaggaattgc aggcgcacgg ctacgcgctg ccggactacc ctgacgagcc gcagggcgag
    18481 cgcgagaagg acatcaaggc gcgctatgcg aaggcgatgg gcagcgcggt caacccggtc
    18541 ctgcgcgagg gcaactccga ccgccgcgcg ccggcctcgg tgaaggccta cgcgcgcaag
    18601 catccgcacc gcatgggcaa gtgggacggc gggtcggcct cgcacgtgtc gcacatggag
    18661 gccggcgatt tctacggcag cgagcagtcc tacacgatgg tcaacgcggg tccgctgcgc
    18721 atcgagtaca ccagcgtcac cggcagcagc ttcccgctgc gcgggccgct gcaggtgcag
    18781 gccggtgaag tgatcgatgc ctcggtcatg agcgcgcagg cgctggccga tttcatcgat
    18841 gcgcagatcg aggacgcgaa ggcgcgcggc gtgctgttct cgctgcacct caaggcgacc
    18901 atgatgaagg tcagcgaccc gatcatcttc ggcatcgcgg tcagccgctt ctacgcgccg
    18961 gtgctggaga agcacccgcg gacgatggcc gagatcggct tcgaccccaa caacggcatc
    19021 ggcgacctgt acgccaagct gggccagttg ccggaggcgc agcgcgcggg catcgaatcc
    19081 gacatcgccg cgctgtacgc gcagcgcccg ggcctggcga tggtcaattc cgacaagggc
    19141 atcaccaacc tgcacgtgcc cagcgacgtg atcgtcgacg cctcgatgcc ggcgatgatc
    19201 cgcgacagcg gcaagatgtg gaacgcggcc ggcgagttgc aggacgccaa ggcggtgatc
    19261 ccggaccgct gctacgcggg catctaccag gaagtgatcg acgattgccg cgagcacggc
    19321 gcgttcgacc cggcgaccat gggcagcgtg cccaacgtgg gcctgatggc gcaggccgcc
    19381 gaggaatacg gcagccacga caagaccttc aagatcgccg gcgacggcac cgtgcgggtg
    19441 atcgacgaac acggcaccgt gctgttgcag cacccggtca aggccggcga catctggcgc
    19501 atgtgccaga ccaaggacgc gccgatccgc gactgggtga agctggcggt gactcgcgcg
    19561 cgcctgagca acacgccggt ggtgttctgg ctggatccgc gccgcgacca cgaccgcggc
    19621 ctggtggcca aggtcgctgc gtacctcaac gaccacgaca ccagcggcct ggacatcagc
    19681 atcatgagtc cgatccgggc gatgcggcac tcgctcaagc gcatccgcca gggcctggat
    19741 accatcgccg ccaccggcaa cgtgttgcgc gattacctga ccgacctgtt cccgatcatg
    19801 gaactgggca ccagcgcgaa gatgctgtcg atcgtgccgc tgatgaacgg cggcggcctg
    19861 ttcgagaccg gcgccggcgg cagtgcgccc aagcatgtgc agcagtacgt cggagaggac
    19921 tacctgcgct gggattcgct gggcgagttc ctcgccctgg cggcctcgtt cgagcatctc
    19981 gccaacacca ccggcaacgc ccgcgcgcag gtactggccg atgcgctgga tgccgccaac
    20041 gcgaagttcc tggacaccga caagtcgccc ggccgcaagc tgggcacgat cgacaaccgc
    20101 ggcagtcatt tctacatcgc gctgtactgg gcgcaggcgc tggcggcgca gaccatcgac
    20161 gccgagctcg cggcgaagtt cgcacccctg gcacgggcgc ttggcgatgg cgaagcgaag
    20221 atcgtcgagg aactgatcgc cgtgcagggc aagccggtcg atatcggcgg ctactaccag
    20281 ccggacctcg acaagctgtc ggccgcgatg cgtcccagcg cgaccctcaa cgccgccctg
    20341 gccaccctgg ccggctgagc ctgcccggcc acgctgtcct cgcgaagccc ggcacccgcc
    20401 gggcttcgtg tttcacggcc gacgccggcc atgccgcgct acccttgccg cctccgccca
    20461 ccggaacacc accatgcccc gattccacgc ctccctgctt gccagcggcc ttgttgtcgc
    20521 attcgccgcg tgtgcatcga ccacggcaac gccagcagac accgcgacgg ccaccacgca
    20581 agccgatgcc gccctcgatg ccgccatcgc ccatcccgcg cgcgacccga agaacgtggc
    20641 ccgcgacgtg caccggcatc cgcgcgaaac gctcggcttc ttccaggtga agcccgacgc
    20701 cacgatcatc gagatcagcc cgagcggtgg ctggtacagc gagatcctcg ccccgtacct
    20761 gcgcgatgcc ggccgttacg tggccgccac cgcctccgcg ccggccgaca gcggcggcgg
    20821 caagcgcaac gccgcgctgc aggcgaagtt cgcggccgcg ccggcgcgct acgaccgcgt
    20881 gcaatggctg gaatacgacg gcaaggcacc gatgctgggc gcgccgggtt ccgccgacgt
    20941 ggtgctgacc ttccgcaacg tgcacaactg ggtggccgcc ggcaccaccg atgcctactt
    21001 ccaggcgttc ttcgcggcgc tcaagccggg tggcgtactc ggcgtggtcg atcaccgcgc
    21061 caggccgggc accggcctgg aggcgatgaa gacgtccggc tacctgaccg aagccctggt
    21121 catcgaccat gccacccgcg ccggcttcgt gctggaggcg cgcagcgagg tcaacgccaa
    21181 tccgaaggac acggccgacc atccgaacgg cgtgtggacg ctgccgccga ccaatcgcca
    21241 cgaccccggc gacgccgcga agtacgcggc catcggcgaa agcgaccgga tgaccctgcg
    21301 cttccgcaag cccgcccgct agcaaacacg cgcaccgcgt attgcccgcc gcgcgcgcgg
    21361 cgggcagcgg tggtccacga tgccctttcg ctccgcccgc cggccatgct gatcgcgttc
    21421 aacaagccgt atggcgtgct ctgccagttc acggacgatg cccatggacg ggcgagtgcc
    21481 gcgcgcgcag gacgcgcaga agcggcccgc agcacgccgg cgcggcccac gctggccggc
    21541 ttcggcctgc cacccggcgt gtatccggcg ggtcgcctcg attccgattc cgaaggcctg
    21601 ctgctgctca ccgacgacgg gtcgctggcg catcggctca ccgacccgcg gcacaagcaa
    21661 cccaaggtct acctcgccca ggtcgagggc gatccgtgcg atgcgcagct gcaggcattg
    21721 cgcaacggcg tgcagctcaa cgacggtccg accctcccgg cgcaagcgcg acgcatcgac
    21781 acgccagcgc tgtggccgcg cgatccaccg gtacgcatgc gcaagaccgt gcccgacgcg
    21841 tggctggaac tgaccctgcg tgaaggccgc aaccggcagg tcaggcggat gaccgcggcg
    21901 gtcggcctgc cgaccctgcg gctggtacgg atcgccgtgg gcgggcacct gctggcggga
    21961 ctggcgcccg gccaatggcg ggacatttgc gcgggttgag ccgaaagctg aatgcttcag
    22021 ctttgtactt tggtcgggct atcctgccgg cgggcccttt gttaacgtcg ccaccgccga
    22081 ttcggcgtgg ggaacgcttt tcggatgccg catcagattc caagaaccgg ccggatcctg
    22141 gtcgtcgacg accaggccgc caacgtgcgc gtggtcaccg cgctgctgga acgccacggt
    22201 tacgccgtca ccgccgccag caatggcaag gatgcgttgc aggccgcgac cggcgaagtc
    22261 cccgacctgc tgctgctgga catgatgatg ccgggcatgg acgggttcga gctgctcgcc
    22321 gcgctgcgcg aactgcccgg tttccgccag gtgcccaccg tgttcctgac cgccgcccag
    22381 gatcgcgacc tgctgctgcg cgcattcgag gccggcgcgg tcgactacgt caccaagccg
    22441 ttcatgcccg aggaattgct ggcacgggtc agtgcgcacc tgggcctcaa gctcacccgc
    22501 gaccgcctgg aacgggtcgc ccgggagcgc caggagctgg tcaacctggt cgcgcacgat
    22561 ctcaagaacc cgctgaccag cgtgctgttc gccaccgaga tgctggcgct gccggactgc
    22621 aagccggagc gcgtcccgcg ctacatcgag atcatcgatg gcagcacccg cgacgcactc
    22681 ggcttcatcc gcaattacct ggaagggcag gaacgcgcgg ccagcagcaa ggccgctgcg
    22741 gtcgccacgc cctgcacctg gatgggcgaa accctgggtt ggctggcggc gcggtatgaa
    22801 ctgcagctgg aagccaaggg cctgcgcctg caggtcgctc cgctggaacg cgacgccagc
    22861 gtggccatcg acggcctggt gctgcgccag gtcgccgaga acctggtcac caatgccctg
    22921 aagtacgcgc gcgacggcgg cgaactggaa ctgcttgccc ggcccggtgc gcccgggttc
    22981 tggcaactgg tcacgcagga tcgcggcccc ggcattccac cggcgcggca gcgcgaactg
    23041 ttcaagccgt tccatcgcct gaccgaggtc gatccggcca atggcctgtc cagcggcttg
    23101 ggcctgtcgc tggccaagca gatcatcggc aatgccggtg gccagctctg gtacgaggac
    23161 cgcgaaggcg gcggcgcgcg tttcgtgatc gaactgcccg aagccgcctc cagcccctcc
    23221 gccggctgat cagtcggcgg tctgttccgc tgccggcggc ttgcggcgcg tgcgcgacga
    23281 cgccttgccg cgcttggcct cgacccgcgt cgcggtgcct tcgatcggtg caccggcggc
    23341 ctgcttcttc gcacgctgcc gcgcggccag ccacagcagg ccggcaccgg cgatcgcggc
    23401 acccgccaac accgggttgc gccgcacgaa cttgcccgcg atccgggtgc cggtgcgcac
    23461 cgcgccgagg gcggcgccgg tttcgaccca ttgcatcgcc ttgcccggca cggccttgcg
    23521 gatgccgtcg cccagttcgc cgatcagttc cagcgcgcga tcctgcgcat tgcccagctt
    23581 gctcatggtg gtgcctcgga tggtggggtg acagttgcag gaagtctctg cagcgcggtg
    23641 ttcatggcgc gtgagcgggg ttgcggatgg ttcagcctga ggccgcgcat cccggcgttg
    23701 gccgggatgc cgacaagagc atgacagcaa gcaggaggtc tcaggtgccc ctgggcttcg
    23761 cccgatgcgt cgccaccgcg ttccacggat catcgggcca cgggtgccgc gggtagcgcc
    23821 ccttcatttc cttgcgcacc tccggatagg tgcgctccca gaagccggcc aggtcctggg
    23881 tgacctgcag gggccgcccg cccggggaaa gcaggtggat ggtcagcgcg atgcgcccac
    23941 cggcgatgcg cggcgtctgc gccaggccga acagttcctg cagcttcacc gccagcaccg
    24001 gcgatgccgg ttcgccgtgg tcgtcgagtg cgtaggcgat cgcgcgctgc atgccggagg
    24061 ggacgtcgat gcgcgtcggc gcgagctggt cgatgcgttg gcgcaggttc cagtcgacgg
    24121 cggatttcag cgcttcggcc agcgccgatt cgtcgagcgc atccagccgg gtcttgccgg
    24181 cgaaggccgg catcagccac gcgtcgagcg aggacagcag cgcggcatcc gacaggtccg
    24241 gcaacccgag ctccggcatc cacgcacgca ggcattgcac gcggacgcgc cattgcgaca
    24301 acgcctcggt ccacggcaat gccgtcaatc cgaggtcgcg cacggcgtcg gtcagcgcgc
    24361 gcgccgccag cgccggatcg acgcgcccgg ccggcttgct cgacagcacg atgccgtcga
    24421 agcgggcgat gcgttcgctg gccagcgcgc gtcgacccgc atcccagcgg acctcgtcgc
    24481 cttcgtggaa atgcgcggcg aacgcttgcc gcaggtatgc ctcgtcgacc ggggcggcgc
    24541 gcagcaagag cgcgtccctc gcctcgaagc gcaactcgct ggcgaccagc cacggctcgc
    24601 catgcaccgc ggaatcgtcg aacagcttcg ccatccgccc gttcgccagc tggtagcgct
    24661 tcgcgtcctg cggatgctgc cgggcgatgc ggtcgggaaa ggcatgcgcg agcaggtcgc
    24721 cgaggtcgtg agccggcaca tccgcgggtg gatgcgcatt gcagcgcaag cggcgacgcc
    24781 attgcttcga tgccgcatcg atcgccgcca gtgccgagcg attcgcgtca gcgccgacgc
    24841 gtccgttgcg gaacgcggcc agcgcctgcc agcgctcagc gagcgcatcg ctgcgactgc
    24901 gcagcggatc ccgcgcttcg atcaatgcgg ccaagtcgca ggccagcgcg atgcgcgagg
    24961 gctcgttcga tacgagcagc atcgccgcca gtcgcggatg cgtgcccagg gccagcatcc
    25021 gacggccgcg ggcggtgatc gcgtgcctcg catcgagcgc gtcgagccgt cgcagcaagt
    25081 cgcgcgccgc ggccagtgcg ccggtcggtg gcggatcgac gaagcgcaag gcatcgctgc
    25141 cccaggccgc gagttcgagc gcgagcgagg ccagctcgac ctgggcgatc tccggccgcc
    25201 gctgcggctc cagccgttgc gactgcggcc acagccgata cgcgaagccc tcggcgacgc
    25261 gacccgcgcg acccgcacgc tggtcggcgg aagcctgcga gatcgacacc acgtccagcc
    25321 gcgcgaagcc gctgttggga tcgaagcgcg gctcgcgggc gagaccgctg tcgatcacca
    25381 cgcgcacgcc gggcaaggtc accgacgatt ccgcgacatt ggtcgccagc accacgcgac
    25441 gccggccatc ggtggcgggt tggagcacgc gggtctgttg ttccaacggc agctctccgt
    25501 gcaagggcag cacgtcgacg tctgcaagct gcgatgtcgg tggctggggt gttgcgtcgt
    25561 gcgcgccatg gcgggcgcgc gcgcgaatcg gggcaggatg ccccgactga gggaaaagca
    25621 attccccagc cgcaggaagc gcagccgcga ggagccgctc cacgttcgcg atctcgcgct
    25681 ggccgggcag gaacaccagc acgtcgccgg gatgcgtcac cagcgcctgt tcgatcgcac
    25741 gtttggtttg gtgctcgatc gcttcctcgc ggcgcgcggg gaagtgcgcg atctcgaccg
    25801 gatagctgcg gccggcgctg ctcaggcgag gggcatccag gaacgtcgcg aggcgctcgc
    25861 catccagcgt cgccgacatc accacgatcc gcaggtcgtc gcgcaagccc gattgcacat
    25921 cgagcgcgag cgccaacccg aggtcgccgg cgagatggcg ttcgtggaat tcatcgaaca
    25981 gcagcgcgcc gacgccttcc agcatcggat cgtcctgcag catgcgggtg aggatgcctt
    26041 cggtgaccac ctcgatgcgg gtgttcgggc cgactttgtt ctcgaaacgg atccggtaac
    26101 cgacggtctc gccgaccgct tcgccgaatt gcttcgccat gaaaccagcg gccgcgcgcg
    26161 ccgcgacccg gcgtggttcc agcatcacga tcttctgccc acgcagccag tcggcatcca
    26221 gcaacgccag cggcacctgt gtggtcttgc ccgcgcccgg cggcgcttcc agcaccagcc
    26281 gcggatgatc ctgcagcgat ccgcggatcg ccggcagcag ggattggatc gggaaagcgt
    26341 tttcactcat gcgactaggc taaccgcccg cagccacggt gcactgcggt atgactttcg
    26401 tcacatttcc ggggcaagat cgcgccactt tttgccgcga gagtcccccg tgtcgccacg
    26461 ccccgtccgt tcccgccgcg cctggttgtc ctgcgccgcg ctcgcccttg ccttcgtcgc
    26521 cgcgcccgcc tcggccgatg tcttcatcaa cgagatccac tacgacgacg cgacctcgtc
    26581 cggcgacacc ggcgaacgca tcgaagtggt cgccaccgcc ggcgagaccc tgagcgccta
    26641 tcgcatctat ctctacaacg gttccacgcc gtcggccgcg gtgacctacg acaacgacct
    26701 ggtgcccgcc ggcagcctgg tctcctgcgg cgcgcaggtg cggatcgcca ccgtcagcta
    26761 tgcgagcaac ggcatccaga acgggccaaa cgatggcatc gccctggtca atggcgccgg
    26821 ccaggtcgtg cagttcatca gctacgaagg cgtgatcaag gcgagcaacg gccccgctgc
    26881 cggccgcacc agcgtgaacc tgccggtgtc cgaatccggt tccgatgcgg ccggtcgttc
    26941 gctgcggctc accggcagcg gcaccaccta cgccaacttc agctggcaac ctgcggcgac
    27001 cgcgagcttc gggtcctgca acgcaggcca gaccttcgcc gcgccgaatc cgccgccggt
    27061 ggtgacttcg accacgccgg tcgccggcgc caccgatttc cccgccgcgg gcaacctggc
    27121 ggtcggcttc agcgaatccg tgagcctggc cagcggtgcg ttcaccctcg cctgcagcac
    27181 ctccggcaac gtcgccctta cccacgccac cagcggcgcc agcttcccca tcagcacgaa
    27241 caccgcgctg catggcggcg agacctgcac gctcggcgtg gtcgccagca aggtgacgga
    27301 cgctggcggt gccaagccgg tcgccaacac cacggtggcg ttcaccgtcg ctgccgccgg
    27361 cccgaccggc tactacagcc acgtcaacac cagcagcccg tcgcagttgc gctgctcgct
    27421 gcacgacacc atcaagggcc acaccgccta tccctacagc ggcggcacca ccaatacctg
    27481 gacgatcctg gagatcgccg acgaggatcc gaacaacagc ggccgcatcc tcgatgccta
    27541 ccgcaaccgc agctacctca aggtcaccga acgcgccagc agcggcagcg gcctgcgcta
    27601 caaccgcgag cacacctggc cgaactcgct gggcttcggc tccgccaccg gcaacctggg
    27661 cctgccgtat gcgccgtaca ccgacaccca catgctctac ctcaccgatg ccacgtggaa
    27721 cgcggaccgc ggcaacaagc cgtatgccga ttgcagcagc agttgcggcg agcgcgtgac
    27781 agaagccaac aacggcgtcg gcggcggcag cggcgtgtat ccgggcaact ccaactgggt
    27841 gcaatccccg gacggcaata ccggttcgtt ccaggtctgg ggcaagcgca agggcgacat
    27901 ggcgcgcgcg gtgctgtaca tggcgatccg ctacgaaggc ggcgtggatc cgaacaccgg
    27961 gcaatccgag cccgacctgg aactcaccaa cgaccgcagc aagatcgtca tcacgtccgc
    28021 gtcgccggcg tacatgggcc tgctctcgac gctgctggcc tggcacgcgg ccgatccgcc
    28081 ggacgccgcc gagcgcgacc gcaacgaagt catccagagc ttccagggca accgcaaccc
    28141 cttcatcgac caccccgagt gggcgacgaa tgcgctgttc acctcgacca agccggcgac
    28201 ctgccagttg aactgagggc cgaaaaacgg cggcatcggc ggcgagaccg tcggatggtt
    28261 cgcgtagcgg catttcgagc cgcgtagcga accgtccggc ggtaggcgac gccgaggcgg
    28321 gagacttcac gctcttacaa tgcgcgaatg aacctcatcg acatcggcgc caacctcacc
    28381 cacgacagct tcgaccgcga ccgcgacgcg gtcctgcagc gcgcacgcga tgccggcgtg
    28441 gcccggatga tcatcaccgg cgccagccgc gagcattcgc cgaaggcgct caccctggcg
    28501 caggcgcatc ccggcgcatt gttcgccacc gccggcgtgc acccgcacca cgcggtggaa
    28561 tacaccgagg aatgcgacgc cgagatgcgc gcgctgcatg cgcatcccga agtcgtcgcg
    28621 gtcggcgaat gcgggctgga tttcttccgc gatttctcgc cgcggccggc gcagcgcaag
    28681 gcgttcgaac gccagctgca gatcgcggtg gagacgcaga agccgctgtt cctgcaccag
    28741 cgcgatgccc acgccgactt catggcgatg atgaagaact tcgacggcaa gcttggcccg
    28801 gcggtggtgc actgcttcac cggcagccgc gaggaactgt tcgactacct cgacaacgac
    28861 tggcacatcg gcatcaccgg ctggctctgc gacgaacgtc gcggccagca cctgcgcgag
    28921 atcgtcggca atatcccggc gcatcggctg atggtcgaaa ccgacgcgcc ctacctgttg
    28981 ccgcgcacat tgaaaccgat gccgaaggac cgacgcaacg agccggcctt cctgccgcac
    29041 atcgtcgagg aactggcgcg cgatcgcggc gaggacgtgg ccatcgcggc ggccaacagc
    29101 gcggccacgg ctgccgcgtt cttccgcttg ccgggtgcgt gaacggcata ccgcatcgac
    29161 ccgtgcgctc gtcccgtcgc cgcgatgggt ccttcctgcc tggtcaatgg tgcccgtgtc
    29221 cgccttcgtc ctgctcggcg atgccgcgca cggcgaacgc caccggcagg cggccggcat
    29281 cgcggaacac cagcgtcgcc tgcagttgtt cgccggctgt ggcgtggcgc acgggttcga
    29341 tgaacatcgc atggtcgcca ccgggcgcaa gcacgacggt ctggccggcc gggatcggca
    29401 cgccctccac caactggcgc atccgcatca cgccatcctc gtgacgcatc gtgtggagtt
    29461 ccacgcgctt cgcggtcggg ctttcgatgc ggatcagctg gtcgtcgcgg ttgcccttgt
    29521 tctggatggt gacgaagccg gcggcgacgg tcgcggtggg cgggatttcc cgcgaccacg
    29581 gcaccgagac caccaggtcg ccgatgcgtt ggtggtcctg ctgcgcgcaa ccggccagca
    29641 ggaaaagcga aagacaaatc agcaacgact tcatcggtcg gtctccgtgg gttcgaggga
    29701 aggaatgccg tcatcgagca aggtgatggg atgcgggtcg gccacgcgcg tcgtgcacca
    29761 ggcggcaccc aggaacacgc cccaggccat cacccacgcc gaaaccgcga acaggaacag
    29821 caccggcagg ttcatgcgcg cttgctccgg caattggcga agtgcgccca tgcgttttcg
    29881 gccagccaca gcagcgcggc ggacagcgcg agcagcggca gcaccaggaa cccgacgagc
    29941 gccagcacgc gcaaccacca gggccatgga cgctggctcg gcggcggcac gcccagcgaa
    30001 cgcgcgggct tgcgtcgcca ccacgccatc gtgccggtga cgcacagcca cgcgagtgcc
    30061 agtgcgaatg cggtgttgac ccagcggccg gatcgcccga agtagtcggc ttcgtggagc
    30121 ttcacgccgg tcgccacggt cttcgccatc aacgggaaac tcgaccagcc ggcatcgtcc
    30181 agcacctgcg cattcgcgcg gtcgagcaac aggtagcgct cgtctgaggc gttcgccgcc
    30241 acgcttcgca gcgacaccgc cgagcccggc gggccgtcga ccatagtgaa catcaggtcg
    30301 ccggccatcc cgcgcgagcg cgcctgctcc agcatctgct gcaggctgcc cacctccgtg
    30361 gcggcctcgc ccgcgaacac cggcgcgaaa cccgccgcgc gtgggccctg ctggcccagc
    30421 gcctgttgca ccggcagcag caccttgttg ccccagaagc tggtccaggg caacgcggtg
    30481 aacaggaaca gcacgatcag cagcgagaac cacaccgcca cgcaagcgtg caggtcgcgc
    30541 cagaaggtcc agccgccgct gcgcaggcgc ggcaacagcg cccgccagcc gcgcccatcg
    30601 cgcggccacc acaggtacag gccgctcagc accatcacga tggtccagca cgcgcccagt
    30661 tccagcagcc agctgccggc atcgcccagc ggccagccgc cgtggaggct gcgcgtccag
    30721 ccgaccggcc acgccgcacc ttccaggctg cccagcacga ccccgcggtg cggatcgacg
    30781 aacaccgcca gcggcagccc actcgcggcc gcgaatgtga cctgggtgct gcgcgccgga
    30841 tccaccggca gcaacacgtt cgccaccttc gcgcccggtt ggaaatcgcg cgcggcccgg
    30901 agctgcacgt cgagcgacac ctgtcgtggt gccggctcga cgaagcgcag gtccgggtgg
    30961 cgctggtcga cccacgcctc cgaccacagg tacagcgtgc cggtggccga ttgccagagc
    31021 acgaacggaa tcaccagcaa cgcggcgagg aaatgccagc gccaggcgcg acgccgcatg
    31081 tcgcgtccac cctgcagttg tgtgtcgatg tccatggctc agaaccgcag ccgcaggccg
    31141 gcgtagaccg agcgcggctc accggcctgc aggatggccg agcctgccga cgcgcgatcc
    31201 agcacgctga agacgccgac gtactccctg tccagcaggt tgcggacctc gccgaacacc
    31261 tcccagcgct cgcgctcgaa cccgacccgc agcccgagga gttcgtaggc agcgacgcgg
    31321 taggtattgc tgaagtccgc gtagcgcgcc ccgaccacgt cgaaggtcgg gccggcgaag
    31381 aagcccttgt cgttgcgcac catcacttca ccgcgaatgg catggcgcgg cgccgccggc
    31441 aggcggttat cggcgtacac cgcgtcgtcg tcgaacgaga acgcgttgta ggtcgcactc
    31501 agcagcggtt cgatgcgata gccgccaccc agcgggatcg cggctccgaa cagcgcctcg
    31561 atgcccgcat gcacggtgcg atcgacgttg gtcgacaggc tggtgccggg ggcggcgacg
    31621 ttgtcgatcg acaggatctc gtcgtggatc cgcgcgtagt acagcgatgc atcccactgc
    31681 cagcggatcg catcggtcgg ccccgtggtg ttgccgcgca cgccgatctc ggccacggtg
    31741 ccgtgcatcg cgtcgagggt ggcgtcgccg ccgcgcacat cgtcctgcag ctcgaacgtg
    31801 gtcggcgctt cgtacaggcg gctgacgctg gcgaaggcct cgctggccgg ggtcagcgcg
    31861 cggatcacgc cgatgcgcgg gttgaacgag gcatagtcgt ccttcggatg gcgcagcacg
    31921 ccgctggcga catcggtgtt gcgcacgtcg cgaccggtga acacgccctg cgcgccgtag
    31981 accagggtcc acgcgggtgc gatcgtccag cgatccacga cgaacacttc catgctgtcc
    32041 gagcggttgt cgacgatcgt gcgcaagcca ttgcgctggc cgccgtcgtt gcggaacagg
    32101 ccaccgcttt cgtgcgtgtt ggccacgttg aggccagcca gcacatcgtg gtcgccgagc
    32161 ctcgcgttgt aacggagcat gccgccccag gtgcgctggt cggtgttctt cagcaggctg
    32221 aagacttcca ctggcggcat cggacccggg ccgtcgaagt ccaccatgac cttgtcgacg
    32281 atcgggtggt agaggtcctg gtcttcgtac gacaggccga actcgaggcg attgccggcg
    32341 tcggggctcc atgtgccctt cactgcgagg cgatcggtct tcacgttcaa ctggaaattg
    32401 ccgctgatcg ccgaggactg cgcctggtac gggtcctgct cgaactgcgc gcgggtcaac
    32461 gggccggcca actcctcatc gctgtccaca tgggtggcga agacgcgcag gtcgaggtca
    32521 tcgctggcct gccaaccggc attcgcatac aagcccatgc ggtcctgccg gctgtgcgcg
    32581 cgatagccat cgcgctgcag gctgtccagg gtgaccaggc catcgaggtt gcccgacacg
    32641 ccacccgcgg tcagccgtgc cgcgacctga ccgtggctgc cgccgcccag gaacagctgc
    32701 atcgtcgggc tgttgcgcgc ggtgggcgag aggaaatcga tcgccccgcc aaggttgctc
    32761 gcgccatagg tcagcgcatt cgcaccgcgg gcgatcaccg catgccgcgc cgccagcggg
    32821 tcgaggaagc ggttgtggtt gttgccgtcg gcggtggtga ccggcaggcc gtcctggaac
    32881 agcttgatgc cgttgctgtc gtagttggtg gcatcgaggt tggagccgcg gctggagatg
    32941 aagatggcgt cgccgccggt gccgctctgg gtgaggacgc cgggcacgta gcgcagcgca
    33001 tccgccatgt tggtgacggc gcgttgctcg aaggtctcgc catcgaccac ggtgacgcca
    33061 ccgggggtca gcgcgcgctc ggcttcgaca ccattttttt cggcgcggac ttcgaccttg
    33121 cccagggtcg cgacctgggg atcggcatca tcggaagggg gatcgccggc gaaggcggtg
    33181 acggggcaag acagcgcggc gagaacggca agcgacaggc acgacgaacg catcgccgtg
    33241 cgggaaggaa gggacatgtt ggctccaaag cacaagaacg cgacacgacg gtcgcgagga
    33301 aaggcgtggc ttgtggttca gagcgtgagc gggggtccct gtgcgccgag tccgcgcagg
    33361 ttgcgcggcg tgcgtggcgc atgggtgcgg cgccggaaca ggggtgcgat gggcggcaat
    33421 gccagcagcc cgcacaacag caagagcagc aacggcagcg gcgtgaccag gctgcaatag
    33481 ccgcaggcat cgccgggctc gtgcggatgc ggggcggggt gttcgtcgcc accgaagccg
    33541 gcggcatcgc tgccgagcaa cggtgcgatc aggcgggtct gcaggcccgc ggaggtgcac
    33601 atctgcagcg cacgcagcac cgcgccattg gcatccggca gcgtggccag cgaacggctg
    33661 atggtgggcg cgagcgccat cagcaacatg gccgccaggg ccagtcgcgc catcaggttg
    33721 cggaaggtag tggagcggat cacccgcgca ttctacgccc gggcgacgac gatcagctgc
    33781 gcaggccgac gccgcgcttc aacagccaca gcgccagtgc gccgagcacg ccgacgaaac
    33841 ccagcatcaa cccatacgcc gtccacatcg acacgtcgct gacgccgagc aggccgtagc
    33901 ggaaggcatt gaccatgtag aagatcgggt tggcgtgggt ggcggcctcg gcccagccgg
    33961 gcagcagctt gaccgaatag aacacgccac cgaggtaggt gagcggagtc aggatgaagg
    34021 tcgggacgat ggcgacgtcg tcgaacttct tcgcgtacac cgcgttgatg aa
//
LOCUS       DAOOCX010000008        33975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_957_length_33975_cov_8.496167, whole genome shotgun sequence.
ACCESSION   DAOOCX010000008 DAOOCX010000000
VERSION     DAOOCX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 33975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33975
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_957_length_33975_cov_8.496167"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PK399_01400"
     CDS             <1..206
                     /locus_tag="PK399_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW11814.1"
                     /translation="PIPVDYLLRNVNGQWKLFDVLVEGISYVQAFKNQFAAPLTRMSI
                     RQVAADLRAGRMQAAPAPGKKAK"
     gene            203..496
                     /locus_tag="PK399_01405"
     CDS             203..496
                     /locus_tag="PK399_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPW11815.1"
                     /translation="MIEAASRVHRDGERLVLSGALDRDAATLSWSRAVAAADGARILD
                     VSAVTSVDSAGLAMLVALAERMQDARIEGAPARMSDLQAAYRLRPTLAFDDGR"
     gene            519..1508
                     /locus_tag="PK399_01410"
     CDS             519..1508
                     /locus_tag="PK399_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HPW11816.1"
                     /translation="MMRLRSLALLLMCALASACATTGHRTADTATSPDAPVIADDVAA
                     ADPSMPSVVGDVEPAMSKASVIADATQAQDAGTDATTGDAEADFAAIYGAPGNDNRAA
                     MGGMPAPAVYDPWEKVNRRVHAFNMAVDRGLAKPLARAYIAAVPRPMRVGVGNFFNNL
                     GQPVTVVNSLLQGDPRGAGNALGRFLVNSTVGIGGLFDVATKANIPRRGEDFGQTLAV
                     WGWQRSRYLELPLLGPRTVRDLIGMAGDARLRPVQYVEDDKTRVFLQGLQLVDLRAKL
                     LPLEGLMRDGAVDEYVLFRDLWMQRRNYQIQQDGRSPDAPAADETMVPADPAD"
     gene            complement(1602..2060)
                     /locus_tag="PK399_01415"
     CDS             complement(1602..2060)
                     /locus_tag="PK399_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11817.1"
                     /translation="MNVKKDHSIGEGTGAVAGAIAGAAVGSAAGPVGTVVGAIAGGAL
                     GAKGGGAVAEAVNPTEYTTHFEKSYKTTPYYISGSEWRDYEPAYKYGYDTYGQYQGRK
                     FEDVETDLERNWDTTKAESRLAWNEARSAVRDGWHHIERAMPGDLDRDGR"
     gene            complement(2232..3620)
                     /gene="rmuC"
                     /locus_tag="PK399_01420"
     CDS             complement(2232..3620)
                     /gene="rmuC"
                     /locus_tag="PK399_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPW11818.1"
                     /translation="MDSTTLLLVVLLIAVLAAIGLLAVLVLRRPEERIGGLLRDEQRA
                     GRGELREQLDGFALQQGQRIDGFGGQLQAVAARTDQRLDDFTLRTDARLDELRAALTE
                     DARKARSEASEQQQRHADALGQKLQELTQRNELRIGEMRATLEQQLKSLQADNAQKLE
                     QMRATVDEKLQSTLEARLGQSFKLVSERLEAVQRGLGEMQTLAAGVGDLKRVLTNVKD
                     RGGWGEIQLENLLEQILTAEQFARGVKVRPGSNETVDFAIRLPGQQDGGTPVWLPIDA
                     KFPREDYERLLDAQERGDTDAMRTTALQLERAIKIQGKSINEKYVAPPYTTDFAVLFL
                     ATEGLYAEAIRRPGLVDALQQHHRIVIAGPTTLAALLNSLQMGFRTLAIEKRSSEVWQ
                     LLGAVKSEFGKFATVLESADKQLNTVQNSLKQAGVRTRQIERKLKGVESLPGSDSQPL
                     LGIDDDVDTDES"
     gene            complement(3660..4367)
                     /locus_tag="PK399_01425"
     CDS             complement(3660..4367)
                     /locus_tag="PK399_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HPW11819.1"
                     /translation="MAKLSTPHHERHRSEHIGWLRASVLGANDGLISTSALLVGVASA
                     DATPQVILLTGVAALTAGALSMAAGEYVSVSSQADTEAADTAKERLELESAPEAELAE
                     LTHIYVQRGLDKALAQQVARQLTAHDALGAHLRDELGISQAFAARPVQAALASAAAFA
                     TGALPPLLLAWQWRGGGLSAAITVVTLLLLAVLGALAGWLGGASMVKGALRVLFWGAV
                     ALGVTAGIGHLFGVTAA"
     gene            complement(4369..5553)
                     /locus_tag="PK399_01430"
     CDS             complement(4369..5553)
                     /locus_tag="PK399_01430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPW11820.1"
                     /translation="MAEPLPVVRLKNAWHSTHPWIFQRLVEKPAQRPKPGSIVDVVGV
                     DGVWIGRGFYNGHSRIALRMLETDPDVEVDAGWFSRKIGEAIRLRREFLRLDDVSNAW
                     RVVHSEGDGISGLVVDRYDDLIVVEFFSAGAYRHREWIFNALREHFPGCRFHSFADEH
                     VQKQESFDYKPTLGTQPATVTEHGIHFRADPAGAHKTGFFADQRENREWLSRLCAGKR
                     VLDLCCNTGGFGVYAAARGASEVVGIDIDAAVLDLARENAHLNSALIGSRKPRFIQAD
                     IFPWLRDAANNGERFDVVILDPAKMTRDREQVIQALKKYLDMNKAALAVVKPGGLFAT
                     FSCTGLVSEEQFTDMLRRAAFYAGRTVQVLKVAGAGPDHPWLAQVQESRYLKAVFCRV
                     LD"
     gene            5771..6724
                     /locus_tag="PK399_01435"
     CDS             5771..6724
                     /locus_tag="PK399_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPW11821.1"
                     /translation="MESIGTPFLWLMFAGVVVVALLVDLVLMRHGGPHKVTFKEATWW
                     SLGWVALALAFNAWLWWYSGQQLGDAEGNRIGMEFLTGYLVEKSLAVDNIFVFLMIFT
                     YFAVPEEQKQRVLVIGIIAAIVLRAILIFAGAFLLAKFHWILYVFGAFLLLTGIKMIM
                     AAGKAPDLEQNPLLRWLTGHVPMTRSYQGSALSIREGGKRLYTPLFIVIVMIGITDVI
                     FAVDSIPAIFAITDDPFIVLTSNVFAVLGLRAMFFLLQGMADRFHLLPYGLALVLMFI
                     GVKMLLVDVYKIPIGLSLGIVAATIGTTIALSLLRPPPPAR"
     gene            6807..7571
                     /locus_tag="PK399_01440"
     CDS             6807..7571
                     /locus_tag="PK399_01440"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPW11822.1"
                     /translation="MSTSLTTEQQRLADRSRLRRALNASLGFVLLLAACFAAQHGFDF
                     RFLTVTPRSVEGLLGVLAAPLLHGSIEHIVANGIALLMLGTLAGTVYPKATLRALPLL
                     WIGSGLGAWVLGNPGSHHLGASGVTHGLGFLVFVLGLLRRDRAAIAAGMLAFLFYGGM
                     LMTILPREAGVSWESHLGGAIGGVIAAFLFRRADPQADKPKYSWEIEEELAREQGDAD
                     RAMYETDAPRDVPVLWHRDAAQAERGKVLPFRRPGE"
     gene            complement(7573..8223)
                     /locus_tag="PK399_01445"
     CDS             complement(7573..8223)
                     /locus_tag="PK399_01445"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HPW11823.1"
                     /translation="MTSTAPPIALPPTRSERWLLAGITVLALVASFLIAKDRLTWCLE
                     VIWAMVGLALIAWRWSRFPLTRLLCWLLVLHALVLIHGGAHTYALTPGGFWLQDLLGT
                     ERNPWDRLGHLMQGFVPAILARELLLRLTPLRRGGWLVYLVLAACLSFSAFFELIEWW
                     AALVYGGEADAFLATQGDVWDTQWDMFLCLCGAATSLLVLSRWHDRQLGAAVLAGR"
     gene            8326..9342
                     /locus_tag="PK399_01450"
     CDS             8326..9342
                     /locus_tag="PK399_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011409779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPW11824.1"
                     /translation="MHRAFATALLASLLAACASTPDVRAPVSVTVTAPPPVKVGIALG
                     GGAAKGFAHIGVIKMLEANGITPVYVSGTSAGSVVGALYASGMDAYAMQEKAFALDES
                     RIRDVSLFSGGVVKGQKLQDYVNDLVGGRTLDKMKKPFAVVATNLANGERTVFVRGNT
                     GQAVRASSSIPGVFEAVAIGKAHYVDGGVVSPVPVDAARELGADFVIAVDISSKADGI
                     ASTTSMLGNLNQSIRIMGQKLGAQELARADIVIRPKVNDIGPADFAAKNRAILEGERA
                     AQAALPQIRAKIAALQAARTAKARQAADADAARQREAERQARCARQQGWMDTLRRDDD
                     CKQG"
     gene            9402..10298
                     /gene="phhA"
                     /locus_tag="PK399_01455"
     CDS             9402..10298
                     /gene="phhA"
                     /locus_tag="PK399_01455"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HPW11825.1"
                     /translation="MNANATPRRIENVQTDRGSLPVYATGTVEQPWDSYSATDHAVWQ
                     QLYQRQREILPGRASDAFLHAQDTMGMTPDRIPKFSDLNRVLADATGWQIVGVEGLLP
                     ELVFFDHLANRRFPVSWWIRRPDQVDYIEEPDLFHDLFGHVPLLMDPMFADYLVAYGK
                     GGVKAHGIGPEALMQLARLYWYTVEFGLIRQPDGLRIYGAGIVSSKGESIHSLESAAP
                     NRIGFDLERVMRTRYRIDSYQQTYFVIDSYEELIEATLPDFTPIYERLREQDAIPAGS
                     VLGTDAVFQRGNGEGWASDGDV"
     gene            complement(10326..11585)
                     /locus_tag="PK399_01460"
     CDS             complement(10326..11585)
                     /locus_tag="PK399_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPW11826.1"
                     /translation="MFDIAVACLVITALAAWFNVRFIRLPNTIGVMAIAMLLSLALLV
                     ASKLGFGALYAYEKQFLRSIDFSDVLMQGMLSMLLFAGALHVDVSLLRSYRWQVGVLA
                     VFGTLVSTAIVGFALYLLLPLVGVGLPLGYCLVFGALISPTDPIAVMGILKSAGAPDN
                     VGLVVSGESLFNDGVGVVVFSLLLGLVVRQEAPGVMDAMHLLAMEVGGGLLLGAAVGA
                     VMFLLLRSVDHYKVEVLLTLAGVLGGYELAQRLHVSGPLAMVIVGLVVGHHGRRLAMS
                     DTTRHHVDLFWELLDEILNAVLFVLLGLEIVLVDFDDTYVLAGLVAVFVALGARLLAV
                     GVPVALAPRWFRLPAGSWQVLTWGGLRGGISVALVLALPGGESRDLLLTMTYAVVVFS
                     ILVQGLSIRAVTRRAFGPRAAPVDSGH"
     gene            11656..13221
                     /locus_tag="PK399_01465"
     CDS             11656..13221
                     /locus_tag="PK399_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HPW11827.1"
                     /translation="MNSPALDLASPAAWWRWARQWRTTRLVAGIDRIAVLEHAHDAGA
                     LGPRYAFMVVMSCGIAALGLLQNSAAVIIGAMLISPLMGPIINLGMGLATFDLRTIRG
                     SLGTLAAGVALALAIAVAIVSLSPLQEATGEILARTRPTFFDLLVAVLSGLAGAYATV
                     TRKGETIVGVAIATALMPPLAVVGYGLAVANWNIAGGAAFLFMTNLLAIALSVTIVAR
                     WYGFGGADSPKQTAWQAALIIASFALLSVPLGLALKRIALQSQTELTVRSSLEARAAQ
                     AGGRVSALRVDTAEDSVAVDAVMMLPRHVNGLETQLQQQLAARTGRPVSLRIREVLTA
                     DDASFALQQSTLAELRRSVTALQDAETVRSAALRAREEQQAGMQARLLPWLGRLERAA
                     DGSHWALWLADGSLPLARAMQMESDINAGRNGDMLPLVVHPPLQALAAIPLPATDDPG
                     ADRMLAIQAWALRRWRASSVQATLVTADDAQAAAWEARLREALSARGIALGELQRERK
                     GEARLQLQAAPAP"
     gene            complement(13235..13750)
                     /locus_tag="PK399_01470"
     CDS             complement(13235..13750)
                     /locus_tag="PK399_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11828.1"
                     /translation="MLLPAVILLSLAACSPPPAPAAPPAPTASAPAPVEAPPAPPATS
                     AATPPASAEPVVAAAGSAIEGTIREGNRPPPALRVCAHPLDGGTPTCIDTVAGATTYR
                     IEVAAGRYVVAGQALEDPGMKFAHAERIRCIRAPCPPDTAMVVDVGAGATASAIDLTP
                     GEALPPASHAG"
     gene            13823..14845
                     /locus_tag="PK399_01475"
     CDS             13823..14845
                     /locus_tag="PK399_01475"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HPW11829.1"
                     /translation="MSNTSISLTRFLIEEQRADRINPDLRLLIEVVARACKTISIAVG
                     KGALGGVLGDVGIGSDASINVQGEAQKKLDVLSNEILMEANAWGGHLAGLASEEMDTS
                     HPIPDKYPRGNYLLLFDPLDGSSNIDVNVSVGTIFSVLRCPDGVTNPVDADFLQPGTA
                     QVAAGYCIYGPSTTLVLTVGHGTHAFTLDREVGSFVLTVRGMRIPEETKEFAVNMSNQ
                     RHWEPPMQAYVGDLLLGSEGPRGKDFNMRWVASMVADVHRILTRGGIFSYPLDRKCAD
                     KGGRLRLMYEANPMSFLVEQAGGAASTGRQRLLEVQPTGLHMRVPVFLGSKAEVEAAV
                     GYHAAR"
     gene            complement(14852..15292)
                     /locus_tag="PK399_01480"
     CDS             complement(14852..15292)
                     /locus_tag="PK399_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW11830.1"
                     /translation="MSAHHILLVEDNPDDVELTRLAFAEAGGAHRLHAVGDGAEALDY
                     LLARGRHADRDGADLPALVLLDLNLPRLDGREVLQAIRADPATRALQVVVLTTSAEPR
                     DVDQAYALGANSYIQKPVEFERFVEAVRQIGRYWLELNLAPGRG"
     gene            complement(15289..16029)
                     /locus_tag="PK399_01485"
     CDS             complement(15289..16029)
                     /locus_tag="PK399_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW11831.1"
                     /translation="MNMDREAQLQARLDAADKRLQALLHGISHDLRAPLRAIDGFASR
                     LSRQLESAGDPAAIESLARISATSARMAKLMDGLVELARAGSAELRPMRVDMSMLADW
                     CASELQDAHPGREAVIDVQQGLEVVGDERLLKTLLAQLLGNAWRFSASRPQVQIAVTG
                     TRNDSGLDLAVRDHGVGFDMTYAARILEPFQRLHGSDEGAGDGIGLAIADAIVARHGG
                     HIRAEAIPGEGATFHVHLRDVPIPGGEQ"
     gene            16237..>17321
                     /locus_tag="PK399_01490"
     CDS             16237..>17321
                     /locus_tag="PK399_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPW11832.1"
                     /translation="MELPPHLFQQASFGLILVGGLYLFISEKLRVDITAMLILLALVL
                     TGVLDSKQALSGFASEPAIIVAAVFVISGALAATGISERLGTWVSRAGGRGEARTIAV
                     VMPLVALLAAFTHHVMVTAMMLPLLLRHARDRQLPASRLLMPMSLAASLGTTLTLVSA
                     PAFLLANDLLQRAGAESLGIFSITPIGIALVVVGTLYMLVARWLLPRRSGERNDEDYL
                     RLDRYRTELLIVKDGRWCTRPMSELQKALGQRFRMLGWLRDGRRRDDLTAASPLLAGD
                     ILLVEAAADELLSLHEDAGLDLNAIARFGDQLEGEGDPQLVQAVVAPGSEFIGRSIRE
                     LDFSRQFHAVIAGLWRRGADMADRLSDA"
     assembly_gap    17322..17421
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17422..17892
                     /locus_tag="PK399_01495"
     CDS             <17422..17892
                     /locus_tag="PK399_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPW11833.1"
                     /translation="RGIDVRIFVMIAGVIPLGIAMEQTGTADLLAQGLSHLVAQWPPL
                     LILLVMFSVAALLTQVMSDAATTALLGPVAIALAQLLDLPPTPFVVCTALGAVVAFLT
                     PIGHHGNLLILGPGQYRFGDFLRIGLPLTVAIALVSAWMARWLWLDGPLWPTLQ"
     gene            17909..18772
                     /locus_tag="PK399_01500"
     CDS             17909..18772
                     /locus_tag="PK399_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPW11834.1"
                     /translation="MTTLIAPRVHDLGGFQVRRAVPSLQARSVGPFVFVDHMGPAHFD
                     AGHGVDVRPHPHIGLATVTFLWSGGMRHRDTLGSLQDILPGDVNWMTAGRGIAHSERT
                     PPALRETGFPLHGMQTWVALPQSHQEVEPSFHHHAAASLPARDHEGARLRVIAGRAFG
                     MESPVKVFADTWNVAVDLIADAELVIPADAVERAVYVLDGEAQLDGTDIPDKHLLVLD
                     RGVRHVLRAKTPVKAMLLGGEPLDGPRHMWWNFVSSSKERIEQAKHDWDEGRFGLIPG
                     DDVERIPLPER"
     gene            complement(18928..19995)
                     /locus_tag="PK399_01505"
     CDS             complement(18928..19995)
                     /locus_tag="PK399_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HPW11835.1"
                     /translation="MAPTRGRGAASRVAGRFETREVRGEDDGWGSVYDGMDDAPSPRT
                     QVTVERARSIISRNQSPDIAFSQSVNPYRGCEHGCVYCFARPSHAYLDLSPGLDFETR
                     LFAKTNAPELLRAELAKPGYVPAPIALGINTDGWQPIERRHGISRACLEVLLETRHPL
                     SIVTKGSALLRDLDLLAELAKHGLVSVFVSITSLDNTLSSKLEPRAAAPHTRLKMLSA
                     LRDAGVPTGVLVAPVIPMITDMHLEQILEAARDAGAQSAGYVLLRLPHELKEVWREWL
                     QLHYPERAGHVMSLVRQMRGGKDYDSTFGSRMRGQGPFADLVAMRFAKTRKRLGFEGV
                     PPLRTDLFVAPGKPSPQGSLF"
     gene            20098..21537
                     /gene="cls"
                     /locus_tag="PK399_01510"
     CDS             20098..21537
                     /gene="cls"
                     /locus_tag="PK399_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPW11836.1"
                     /translation="MPDLLHAWETFWAIPHIRAWLTAGWILYLVWLGLWIVLQKREPA
                     ATLSWLVSLAALPYIGFAIYYLLGPQKITRHRARRLRHRASLAREPEGPGDDSVELRT
                     LAHATTGLAPTTAREVRLLVDGGSKYPALLDAIAAARDHIHLEYYIYCPDRTGTALRD
                     ALVERARAGVKVRLLLDAMGSKQVKPRFFAPLVEAGGELAWFHPAQPWTLWKRPWVNL
                     RTHRKIVVIDGAVGFLGGINITDEEDERLREDAYRDLHLRVEGDVVRALQEVFVEDWA
                     YATESRDFVGDVARAMPAPSPGPIRAQLLSSGPDSPWEAIHRLHVGAIHSAGKRVWLA
                     TPYFVPGEAAMMALTSAALGGLDVRLLVPKMSDSRLVTWCVRSYFDDLLAAGVKVYEY
                     GPRMLHTKALLVDDQLAIIGTANFDHRSFRLNFEASLLFEDAGVAAELAQVIERDFAS
                     APRVRDDRARPLLGARLPEALARLIAPLL"
     gene            21604..21858
                     /locus_tag="PK399_01515"
     CDS             21604..21858
                     /locus_tag="PK399_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11837.1"
                     /translation="MHWLFLLIGFALLVLSFLTTNSVVMVLCWLVALVLFGLWLAAMY
                     SARVTSQERDVSMMIDPAELRRLREQAEARKAAASQDTPD"
     gene            21890..22663
                     /locus_tag="PK399_01520"
     CDS             21890..22663
                     /locus_tag="PK399_01520"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016849424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPW11838.1"
                     /translation="MTALSVNLNKIAVLRNSRGGAEPDVLRAGIACLDAGAHGLTVHP
                     RPDARHIREDDVHALAGLCRSRGVEFNVEGNPFAPPRPGYPGLLALCDAVRPAQVTLV
                     PDGDGQLTSDHGFDFTSDGDALRQLVAAYKTLGCRVSVFVDVGDPLVARAASAGADRV
                     ELYTGPFAEAFARGDADAALRMAADTARLAQAAGLGVNAGHDLSQANLAEFLARVPGV
                     QEVSIGHALISEALYAGLDATVRAYRHIVDAAVAGRVNA"
     gene            complement(22653..23015)
                     /locus_tag="PK399_01525"
     CDS             complement(22653..23015)
                     /locus_tag="PK399_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPW11839.1"
                     /translation="MAGINITPLVDVMLVLLVIFMVTMPIRSESIDLTLPGITPPRAI
                     PPPEPVQLQVQADGSALLDGVRVELPALAAELRAQHAQAPKAGLSLDGSGDAEYRAFA
                     FALAAARNAGYRDIAIRR"
     gene            complement(23258..23671)
                     /locus_tag="PK399_01530"
     CDS             complement(23258..23671)
                     /locus_tag="PK399_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPW11840.1"
                     /translation="MAFSSSGGSGPLADINVTPLVDVMLVLLIIFMVTMPIQSVPVDV
                     DLPQKTLNPPPQPKEPPDPISLRIDAAGQVFWNNTPTPVNALQTMMKTEVERDPTNQP
                     TLEIDTSDDADYGVLAKVLASAKNAEMVKIGFVQN"
     gene            complement(23683..24117)
                     /locus_tag="PK399_01535"
     CDS             complement(23683..24117)
                     /locus_tag="PK399_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPW11841.1"
                     /translation="MAFSSGNDSGGPMAEINVTPLVDVMLVLLIIFMITTPLMNHKVK
                     VQLPEAVLDKKPDEKAGLKTPPVTIAVTEKGELFWNDEPSTRQLIESKLSVEAQKDPQ
                     PVVNIRADKTTKYRLVNDVVKVAQAQGMRKVGFVTTPPKKNI"
     gene            complement(24147..24905)
                     /locus_tag="PK399_01540"
     CDS             complement(24147..24905)
                     /locus_tag="PK399_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002805928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPW11842.1"
                     /translation="MLQETTAAAPAAAGGNNAAALQQMGFDHLITQLDAVGWIVFVTL
                     MVMSLMSWYWIIINFVKNIRLRGRGDNVVGTFWETPNAQDAIRFMEEQPKSEPFSKIA
                     LDAAQAAAHHQRHDGSRLAESLNRSEFVDRALRQAVTRESSGLENGLTVLATVGSTAP
                     FVGLLGTVWGIYRALIKIGASGDASISAVAGPVGEALIMTAIGLFVAIPAVLAYNFFV
                     RLNRVTNSQFDTFAHDLHDFFATGSRVGEIAGKR"
     gene            complement(24983..25654)
                     /locus_tag="PK399_01545"
     CDS             complement(24983..25654)
                     /locus_tag="PK399_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW11843.1"
                     /translation="MTQDLANTEKNDDREGGLSWPRIMGISFAIALHAAALLLLLAPI
                     SPPAQDVEEEDVVNVTFIEPPPPPPPPPPPPPDQPKPLTPPKTLSPPRASPVPPPPED
                     PPVVFNDPSPMDVQAPPPSPPAPPSSVPDIGSSVDASSRAMNPPAYPREEQRRNIQGT
                     TVLIVSIDASGGVLDVEVERSSGNRNLDREAVKAARRWRFNPEVRDGKKIASRVRVPV
                     EFKLN"
     gene            complement(25807..26949)
                     /locus_tag="PK399_01550"
     CDS             complement(25807..26949)
                     /locus_tag="PK399_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11844.1"
                     /translation="MKPTFNRPLATSIAAALALALLAPAANAQQDPTATTGTKTMRQQ
                     RAERMAELGKGKEEAKQAEDKPAAYPNATRTQPDAKSSGKMIKNLQGLQELFEKRDMT
                     AVIARAEEIVAMPSANAYDKSFAYSLAANAAADQDDQAKAAEYFRRAVDANGLDNDSH
                     YTTMFNLAVIQFGLEKYAEALATVDRFLAESKSEKPEHQSFRAGILANLERHDEAAAI
                     YKDLIAKNPNDKRILMNAVAALQNADKQDQANALLEDAYKRGMLTESRELRALYIGYM
                     NASRWDDTQKVIDAGVSKGVLQPGPDLARDYQILAQNAYADDKIPLAIDLYGKAAPMA
                     ADGEAYLNLAKVLEYSGKKAEAKAAAQKALDKGVKKPEEAKGILSR"
     gene            complement(27058..27855)
                     /locus_tag="PK399_01555"
     CDS             complement(27058..27855)
                     /locus_tag="PK399_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPW11845.1"
                     /translation="MKTKMFLAGLLATALVAACATTTSPTGRTQYVGGVSQAQLNRLG
                     EQAFAEAKQKTPQSRDARQVAYVGCVVDAVARQLPAPWNQQRWDYALFENSEANAWAL
                     PGGKVGVYTGIFQVATNQDQLAAVVAHEIGHVIARHHDERITRQLGAAGAVQLLGALA
                     GDYGQLATEGGSVLAQAGFLLPNSRAQETEADVVGQDIMARAGFDPRGAVALWRNMSA
                     AGGARSPEWLSTHPDPASRLRELESRADALLPTMQAARSAGKAPRCR"
     gene            complement(27933..30431)
                     /gene="gyrB"
                     /locus_tag="PK399_01560"
     CDS             complement(27933..30431)
                     /gene="gyrB"
                     /locus_tag="PK399_01560"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPW11846.1"
                     /translation="MSNNESTPGQVPESSPPTKPATSGTYDASSITALEGLEAVRKRP
                     GMYIGNVNDGSGLHHMVFEVVDNGVDEALAGHADAVLVTIHDDGSVSVWDNGRGIPVG
                     RHAKESEKQGRDISAAEVVMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKLLLD
                     IWRDGYHYQQEYRHGEPVYPLKQLEASDRRGTEVRFWPSVTAFNDNVEFHYDILARRL
                     RELSFLNSGVKIELVDERPDGKHDVFEYEGGIRSFVEHLAQLKTPLHPNVISISGEQD
                     GIVVDCAVQWSDSYQETMYCFTNNIPQKDGGTHLTGFRAALTRTLTRYIEQSGIAKQA
                     KVSMSGDDMREGMIAVLSVKVPDPGFSSQTKEKLVTEPVRAVVDAVFAAKLEEFLQEN
                     PNEARAIAGKIVDAARAREAARKARDLTRRKGALDIAGLPGKLADCQEKDPALSELFI
                     VEGDSAGGSAKQGRNRKTQAILPLKGKILNVERARFDRMLASAEVGTLITALGTGIGK
                     DEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYIGLPPLYKIK
                     QGKNELYLKDDAALDAYLAGNAVEGAQLVPAANEPAIEGAALESLLLVFARAREAIAR
                     NAHRFDPIVLESLIDFTPLDAASLAAHTDDARELDALAARLNRGGLGKPRYALQLQPA
                     NETRPAALLVIRRHMGEELTQVLPLSTFEHGELKPLREAAAVLHGLVRGGAQIVRGSR
                     AQAIASFAEAQAWLLEEAKKGRQIQRFKGLGEMNPEQLWDTTVNPDTRRLLQVRIEDA
                     VAADAIFSTLMGDVVEPRREFIEENALKVSNLDV"
     gene            complement(30533..31633)
                     /gene="recF"
                     /locus_tag="PK399_01565"
     CDS             complement(30533..31633)
                     /gene="recF"
                     /locus_tag="PK399_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPW11847.1"
                     /translation="MLIRKLHVQGLRRLVDVEMRPGPGFNLVTGDNGSGKTSLLEAMH
                     LMAHGRSFRGRIRDGLVRSGEPALQVFLEWEQPGQPGRRAGLRHSGSAWEARLDGCQV
                     SQLGELCEALAVITFEPGSHALVDGSSEGRRRYLDWGLFHVERSDAGGDFFSLWRRHA
                     RAHKQRNALLRQGRADAQLDAWEHELAQAGEAITLQREAYVARLQPHVDALIAEILPA
                     AGGLSLSLQPGWRRQELGLADALLLARERDIAQGHTTVGPHRADLRLEFRDLPGRDGL
                     SRGQTKLAALVLLLAQASQLAAHAGHWPVLQLDDLASELDRHHQQRVLQVLAGSGAQV
                     FVTGTDPVPALAALDIPCERFHVEHGVVGRLA"
     gene            complement(31742..32842)
                     /gene="dnaN"
                     /locus_tag="PK399_01570"
     CDS             complement(31742..32842)
                     /gene="dnaN"
                     /locus_tag="PK399_01570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPW11848.1"
                     /translation="MRFSLQREVLLKPLAQVVNVVERRQTLPVLANLLVKVREGQLSL
                     TGTDLEVEMVARTAVDDAQDGETTIPARKLFEIVRALPDGSKVTVTQQGDKITVQAGR
                     SRFSLASLPANDFPSIDEFEATERVNVPEAGLKELIERTAFAMAQQDVRYYLNGLLFD
                     LRDTTLRCVATDGHRLALCETALDGGSGSKRQIIVPRKGVQELQRLLEGGDRTLDLEL
                     GKGHIRVKRDDVTFTSKLIDGKFPDYEAVIPIGADREVKVDREALRAALQRASILSNE
                     KYRGVRVEVSPGLLKISAHNPEQEEAQEELEADTRVDGLAIGFNVNYLLDALGALRDE
                     HILLLLRDANSSALVRGAEHQHARHVVMPLRL"
     gene            complement(33125..>33975)
                     /gene="dnaA"
                     /locus_tag="PK399_01575"
     CDS             complement(33125..>33975)
                     /gene="dnaA"
                     /locus_tag="PK399_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPW11849.1"
                     /translation="GNEMRRINPAARILYLRSNEFYNAFFRALQEKTADQFKRQFQQI
                     DALLIDDIQFFAGKDRTQEEFFHTFNALFDGRQQIILTCDRYPREVDGLEPRLKSRLG
                     WGLSVAIDPPDFETRAQIVLSKARERGALVPDDVAQLIAKKMRSNVRELEGAINKLTA
                     QAGLLGRPVTMEFAQETLRDLWRAQQQAISIANIQKAVADYYGLQLREMLGKKRTRSL
                     ARPRQVAMALAKELTEHSLPEIGVAFDGRDHTTVLHACRQIRHLMESDGKLHEDWDKL
                     IRKLSE"
BASE COUNT         5214 a        11494 c        11491 g         5676 t
ORIGIN      
        1 cgccgatccc ggtcgactac ctgctgcgca acgtcaacgg ccaatggaaa ctgttcgacg
       61 tgctggtcga aggcatttcc tatgtgcagg cgttcaagaa ccagttcgcg gcgccgctga
      121 cccggatgtc gatccgccag gtcgccgccg acctgcgcgc aggccgcatg caggccgcgc
      181 ccgccccggg caagaaagcc aagtgatcga agccgcgtcc cgcgtgcatc gcgacggcga
      241 acggctggtg ctttccggcg cactcgaccg tgatgccgcc accctgtcgt ggtcacgggc
      301 ggtcgcagcg gcggacggcg cacggatcct ggacgtgagc gcagtgacct cagtcgacag
      361 cgcggggctg gcgatgctgg tcgcgctggc cgaacgcatg caggatgcgc gcatcgaggg
      421 cgcgccagcc cgcatgtccg acctgcaggc ggcgtatcgc ctgcgcccca ccctggcctt
      481 cgacgatggc cgctgatgtt cgtgcaaagg atggtccgat gatgcggttg cgttccctcg
      541 ccctgttgct gatgtgcgcg ctcgcgagtg cgtgcgccac caccggccat cgcactgcgg
      601 atacggccac ctcgccggac gcgcccgtga tcgcggacga cgtcgccgca gccgacccgt
      661 ccatgccctc ggttgtcggc gatgtcgagc cggcgatgtc gaaggcgtcc gtcatcgcgg
      721 acgccaccca ggcgcaagat gcaggcaccg acgccacgac gggcgatgcc gaagcggact
      781 tcgccgcgat ctatggcgcg cccggcaacg acaaccgcgc cgccatgggc ggcatgccgg
      841 caccggcggt ctatgacccg tgggagaagg tcaaccgccg cgtgcatgcg ttcaacatgg
      901 cggtggatcg cggcctggcg aagccgctgg cgcgtgccta catcgcggcg gtgccacgac
      961 cgatgcgggt cggcgttggc aacttcttca acaacctcgg ccaaccggtc accgtggtca
     1021 acagcctgct gcaaggcgat ccgcgcggcg caggcaacgc gctgggccgg ttcctggtca
     1081 acagcacggt cggcatcggc gggctgttcg acgtcgccac caaggcgaac atcccgcgcc
     1141 gcggcgaaga cttcggtcag accctggcgg tctggggctg gcagcgttcg cgctacctgg
     1201 agctcccctt gctgggaccc cgcacggtgc gcgacctcat cggcatggcc ggcgatgcgc
     1261 gcctgcgccc ggtgcagtac gtcgaagacg acaagacccg cgtcttcctg caggggctgc
     1321 agctggtcga cctgcgggcg aaactgctgc cgctcgaagg cctgatgcgc gatggcgcgg
     1381 tggacgaata cgtgctgttc cgcgacctgt ggatgcagcg gcgcaactac cagatccagc
     1441 aggacggacg ctcgcccgat gcacctgccg cggacgaaac gatggtaccg gcagatcctg
     1501 ccgactgaca cgcgatccgt gatccgtgat ccgtgaaaaa gaaggccgcc cgagggcggc
     1561 cttcttcatt gggtctggcg aatgacgccg accgaccggg ctcagcggcc gtcgcggtcc
     1621 aggtcgcccg gcatcgcacg ctcgatgtga tgccagccgt cgcgcaccgc gctgcgggct
     1681 tcgttccagg ccaggcgcga ctccgccttg gtggtgtccc agttgcgctc gaggtcggtt
     1741 tccacgtcct cgaacttgcg gccctggtac tggccgtagg tgtcatagcc gtacttgtaa
     1801 gccggctcgt agtcgcgcca ctcgctgccg ctgatgtagt acggggtggt cttgtaggac
     1861 ttctcgaagt gggtggtgta ttcggtcggg ttgaccgctt cggccaccgc gccgccgccc
     1921 ttcgcgccca gcgcgccgcc ggcgatcgcg ccgaccacgg taccgaccgg gccggccgcc
     1981 gaacccacgg cagcgcctgc gatcgcaccc gcaaccgcgc ccgtgccttc accgatgctg
     2041 tgatccttct tgacgttcat gtagtgctcc ttggtgtggg gttcgccggc gctccatgcg
     2101 tcggacgtgg ccaaggatgg cgatggacgc gtgttgtccg cgtcatgcgc gcgtgagcgc
     2161 gtcgtgcacc ttcatgaagc catcagggtt gtgagtcctg ccatgccgca tgcatggcag
     2221 catcgaggca ctcaggattc gtcggtgtcc acgtcatcgt cgatgcccag caacggctgg
     2281 ctgtcgctgc ctggcagcga ttcgacaccc ttcaacttgc gctcgatctg gcgtgtgcgc
     2341 acgcctgcct gtttcaggct gttctgtacc gtgttgagct gcttgtccgc actttccagc
     2401 acggttgcga acttgccgaa ctcgctcttc accgcaccga gcagttgcca gacttcgctg
     2461 gagcgctttt cgatggccag ggtgcggaac cccatctgca ggctgttcaa caatgccgcc
     2521 aacgtggtgg ggcccgcgat cacgatgcga tggtgttgct gcagggcatc gaccaggcca
     2581 ggccggcgga tcgcttcggc atacaagcct tcggtcgcca ggaacagtac ggcgaaatcg
     2641 gtggtgtagg gcggcgccac gtatttctcg ttgatcgact tgccctggat cttgatcgcg
     2701 cgctccagtt gcagtgcggt cgttcgcatc gcatccgtgt cgcctcgctc ctgtgcgtcc
     2761 aacaggcgtt cgtaatcctc acgcgggaat ttggcgtcga tcggcaacca gaccggggtg
     2821 ccaccgtcct gctgcccggg caggcgaatc gcgaaatcca ccgtttcatt gctgcccggt
     2881 cgcactttca cgccgcgtgc gaattgctcg gccgtcagga tttgttccag caggttttcc
     2941 agctggattt caccccagcc cccgcggtct ttcacgttgg tcagcacccg cttgaggtcg
     3001 ccgacaccgg ccgccaaggt ctgcatctcg cccaagccgc gctgcacggc ctcaaggcgt
     3061 tcggacacca gcttgaacga ctgtccgagg cgcgcttcca gcgtgctctg cagcttctcg
     3121 tcgacggtgg cacgcatctg ctcgagcttc tgggcgttgt ccgcctgcag tgacttgagt
     3181 tgctgttcca gcgtggcgcg catctcgccg atgcgcagtt cgttgcgctg ggtgagctcc
     3241 tgcaatttct ggccgagcgc atctgcatgc cgttgctgct gctcgctggc ttcgctgcgc
     3301 gccttgcgtg cgtcttcggt cagcgccgcg cgcaattcat ccaggcgcgc atcggtgcgc
     3361 agggtgaaat cgtccaggcg ctggtcggtg cgtgcggcga ccgcctgcaa ctgcccgccg
     3421 aacccatcga tgcgctggcc ctgctgcagc gcgaagccat ccagttgctc gcgcagttcg
     3481 ccgcggccgg cacgctgctc gtcgcgcaac aggccgccga tgcgctcttc cggacgacgc
     3541 aacacgagca cggccaacag gccgatcgcc gccagcacgg cgatcagcaa cacaacgagc
     3601 aggagcgtgg tcgagtccat cgcgccagtg tagcggcgac ggtcgcagcg cctgcgacct
     3661 caggccgcgg tcaccccgaa cagatggccg atgccggcgg tcacgcccag ggccacggcg
     3721 ccccagaaca gcacgcgcaa ggcacccttc accatcgatg cgccgcccag ccatcccgcc
     3781 aacgcgccca gcaccgcgag caggagcagc gtgaccacgg tgatcgcggc cgacaagccg
     3841 ccaccgcgcc actgccacgc cagcaacagc ggcggcaacg cgcccgtggc gaaggccgcg
     3901 gccgaggcca gcgccgcctg caccggccgc gccgcgaacg cctggctgat gcccagttcg
     3961 tcgcgcaggt gggcccccag ggcgtcgtgc gccgtcagct gtcgcgccac ctgctgcgcc
     4021 agcgccttgt ccagtccgcg ctggacgtag atatgggtca gctccgccag ctccgcttcg
     4081 ggcgcgcttt caagttcaag ccgctccttg gcggtgtcgg cggcctcggt atcggcctgc
     4141 gagctcaccg agacgtattc gccggcggcc atcgacaagg cgccggcggt gagcgccgcc
     4201 acgccggtga gcaggatcac ctgcggggtt gcatcggcgg acgccacccc gaccagcaac
     4261 gcgctggtcg agatcaggcc atcgttggcg ccgaggaccg aggcgcgcag ccagccgatg
     4321 tgttcgctgc ggtggcgttc gtggtgcggg gtggagagct tggccatgtc agtccagtac
     4381 gcggcagaac accgccttga gatagcgcga ttcctgtacc tgcgccagcc acgggtggtc
     4441 ggggccggct ccggcgacct tcagcacctg caccgtgcgt cccgcataga acgcggcgcg
     4501 gcggagcatg tcggtgaact gctcctcgct gaccaggccg gtgcagctga aggtggcgaa
     4561 cagccctccc ggcttgacca ccgccagcgc cgccttgttc atgtccaggt atttcttcag
     4621 cgcctggata acctgttcgc ggtcgcgggt catcttggcg ggatcgagga tgaccacgtc
     4681 gaagcgttcg ccgttgttcg ccgcgtcgcg cagccacggg aagatgtcgg cctggatgaa
     4741 gcgcggcttg cggcttccga tcagcgcgct gttgaggtgg gcgttctcgc gggcgaggtc
     4801 gagcaccgcc gcgtcgatgt cgatgccgac cacctcactc gcaccgcgtg ccgccgcgta
     4861 cacgccgaag ccgccggtgt tgcagcacag gtcgagcacg cgcttgccgg cgcacaggcg
     4921 cgacagccat tcgcggttct cgcgctggtc ggcgaagaag ccggtcttgt gcgcgcccgc
     4981 ggggtcggcg cggaagtgga tgccgtgctc ggtgaccgtg gccggttgcg tgcccagggt
     5041 gggcttgtag tcgaagctct cctgcttctg cacgtgctcg tcggcgaagc tgtggaagcg
     5101 gcagccgggg aaatgctcgc gcagggcgtt gaagatccat tcgcgatggc ggtaggcgcc
     5161 ggcgctgaag aactcgacca cgatcaggtc gtcgtagcga tccaccacca ggccgctgat
     5221 gccgtcgccc tcgctgtgga ccacgcgcca cgcattcgac acgtcatcca gcctgaggaa
     5281 ctcgcggcgc agccggatcg cctcgccgat cttgcggctg aaccagccgg catcgacctc
     5341 gacatccggg tcggtctcca gcatgcgcag ggcgatccgc gagtggccgt tgtagaagcc
     5401 gcggccgatc cagacgccgt ccacgcccac cacgtccacg atgctgcccg gcttgggccg
     5461 ttgcgcgggc ttctccacca ggcgctggaa gatccacggg tgggtggaat gccaggcgtt
     5521 cttgaggcgg acgacgggga gcggttcggc catgggactg ccttttgaca tgcggctaac
     5581 gaagttcggg tattgtcgcc caagaagggg agtaactccg cgcacggccg tcgtcattac
     5641 ggatcccagg atcccggcac cgtggcaggc ctggcgcctg tgagtgagac cttcgccgaa
     5701 cggcgaagcg cgctcttctt ccatgtgccc tgcatggacg cctccgccgg ttcccacggt
     5761 ggagtgcgct atggaatcga tcggtacccc tttcctctgg ctgatgttcg ccggggttgt
     5821 cgtggtcgcg ttgctggtgg acctggtgct gatgcgccac ggcggcccgc acaaggtgac
     5881 gttcaaggag gcgacctggt ggagcctggg ctgggtcgcg ctggcgctgg ccttcaatgc
     5941 ctggctgtgg tggtactcgg gacagcaact cggcgacgcc gaaggcaacc gcatcggcat
     6001 ggaattcctc accggctacc tggtggagaa gtcgctggcg gtcgacaaca tcttcgtgtt
     6061 cctgatgatc ttcacctact tcgctgttcc cgaggagcag aagcagcggg tgctggtgat
     6121 cggcatcatc gcggccatcg tgctgcgcgc catcctgatc ttcgccggtg cgttcctgct
     6181 ggccaagttc cactggatcc tctacgtgtt cggcgcgttc ctgctgctga ccggcatcaa
     6241 gatgatcatg gccgccggca aggcgcccga cctggaacag aacccgctgc tgcgctggct
     6301 gaccgggcat gtgccgatga cccgcagtta ccagggcagt gcgctgtcga tccgcgaagg
     6361 cggcaagcgg ctgtacaccc cgttgttcat cgtgatcgtg atgatcggca tcaccgacgt
     6421 gatcttcgcg gtggactcga tcccggcgat cttcgcgatc accgacgacc ccttcatcgt
     6481 gctgacatcg aacgtgttcg cagtgctcgg cctgcgcgcc atgttcttcc tgctgcaggg
     6541 gatggccgac cgcttccacc tgctgcctta cgggctggcg ctggtgctga tgttcatcgg
     6601 cgtcaagatg ctgctggtcg atgtctacaa gatcccgatc gggctgtcgc tgggcatcgt
     6661 ggccgcgacc atcggcacca ccatcgccct gagcctgctg cgtccgccgc cgccggcacg
     6721 ctgacggcac gctcggttcc atcgatgcaa gcaccgccca cccgggcggt gcttgctttc
     6781 gagcggtcgc gccccagttc gatggcatgt ccacgtcact gaccaccgag caacaacgcc
     6841 tggccgaccg cagccggttg cgccgcgcgt tgaacgccag cctcggcttc gtgctgctgc
     6901 tggcggcctg cttcgcggcc cagcacggct tcgacttccg gttcctcacc gtgaccccgc
     6961 gcagcgtcga aggcctgctc ggcgtgctgg ccgcgcccct gctgcatggc tcgatcgagc
     7021 acatcgtcgc caacggcatc gccttgctga tgctgggcac gctggccggc accgtgtatc
     7081 cgaaggcgac attgcgcgca ttgccattgt tgtggatcgg ctccgggctg ggcgcctggg
     7141 tgctgggcaa ccccggctcg caccacctgg gggccagcgg cgtgacccac ggcctgggct
     7201 tcctggtgtt cgtgctgggc ctgctgcggc gcgaccgcgc ggcgatcgcc gccggcatgc
     7261 tggccttcct gttctacggc ggcatgctga tgacgatcct gccgcgcgag gccggcgtgt
     7321 cctgggagtc gcacctgggt ggcgcgatcg gtggcgtcat cgcagccttc ctgttccggc
     7381 gcgcggatcc gcaggccgac aagccgaagt acagctggga gatcgaggaa gaactggccc
     7441 gggaacaggg cgacgccgac cgcgcgatgt acgagaccga tgcaccgcgc gacgtgccgg
     7501 tgctgtggca tcgcgatgcc gcgcaggcgg agcgcggcaa ggtgttgccg ttccgccgcc
     7561 cgggggaatg aattacctgc cggcgagcac ggccgcgccc agctggcggt cgtgccagcg
     7621 cgacagcacc agcagggacg tggccgcgcc gcacaggcac aggaacatgt cccactgggt
     7681 gtcccagaca tcgccctggg tggcgaggaa ggcgtcggct tcgccgccgt agaccagcgc
     7741 ggcccaccat tcgatcagtt cgaagaaggc gctgaaactg aggcaggccg ccagcaccag
     7801 gtagaccagc cagccgccgc ggcgcaacgg ggtgagccgc agcagcagct cgcgggccag
     7861 gatcgccggc acgaagccct gcatcaagtg gcccaggcga tcccatgggt tgcgctccgt
     7921 gcccaacagg tcctgcaacc agaacccgcc gggcgtgagt gcgtaggtat gcgcgccgcc
     7981 gtggatcagc accagcgcat gcagcaccag caaccagcac aacagccgcg taagcgggaa
     8041 ccgcgaccag cgccatgcga tcagggccag gccgaccatc gcccagatca cttcaaggca
     8101 ccaggtcagc cgatccttgg cgatgaggaa cgaagcgacc agcgcaagca cggtgatgcc
     8161 ggccagcagc cagcgttcgc tgcgcgtggg cggaagggcg atgggcggcg cggtggaggt
     8221 catgcgccga tgctagcgcg tggttcgggc gccgtttcgg gaacgcgcat taagcttcgc
     8281 ccgcccgccg cgattcgtgg ccgacactcc gagaccgaca cgacgatgca tcgcgccttc
     8341 gccaccgccc tgctcgccag cctgctcgcc gcctgtgcca gcacgccgga tgtccgcgct
     8401 ccggtcagcg tgacggtcac cgcgccaccg ccggtcaagg tcggcatcgc cctgggtggt
     8461 ggcgcggcca agggcttcgc ccatatcggc gtgatcaaga tgctggaagc caacggcatc
     8521 accccggtct atgtctccgg caccagcgcc ggcagcgtgg tcggcgcgtt gtacgccagc
     8581 ggcatggatg cgtatgcgat gcaggaaaag gcgttcgcgc tggacgaatc caggatccgc
     8641 gacgtcagcc tgttttccgg cggcgtggtc aagggccaga agctgcagga ctacgtcaac
     8701 gacctggtcg gcggccgcac cctcgacaag atgaagaagc cgttcgcggt ggtcgcgacc
     8761 aacctcgcca atggcgagcg caccgtgttc gtgcgcggca ataccgggca ggcggtgcgc
     8821 gcctccagct cgatccccgg cgtgttcgag gcggtggcga tcggcaaggc gcattacgtc
     8881 gatggcgggg tcgtcagccc ggtgccggtg gatgccgcgc gcgaactcgg cgccgacttt
     8941 gtcatcgcgg tcgacatcag cagcaaggcc gacggcatcg ccagcaccac cagcatgctc
     9001 ggcaacctca accagagcat ccgcatcatg ggccagaagc tcggtgcgca ggaactcgcg
     9061 cgcgccgaca tcgtgatccg tccgaaagtg aacgacattg gcccggccga cttcgccgcg
     9121 aagaaccgcg cgatcctgga aggcgagcgc gccgcgcagg ccgcgctgcc gcagatcagg
     9181 gcgaagatcg ccgcgctgca ggccgcgcgc acggcgaagg cacggcaagc cgcggacgcc
     9241 gacgccgcac gccagcgcga ggccgaacgc caggcgcgct gcgccaggca gcaaggctgg
     9301 atggacacac tgcgccgcga cgacgactgc aagcaaggct gaacgcgggc gatttgcgca
     9361 cgatcaagaa ccggaaaacc ggcgggcgta agatgaaggg catgaacgcg aatgccacgc
     9421 cccggcgcat cgagaacgtc cagaccgacc gcggatccct gcccgtgtac gcgaccggca
     9481 ccgtcgagca gccgtgggac agctacagcg ccaccgacca cgcggtctgg cagcagctgt
     9541 accagcgcca gcgcgaaatc ctgccgggcc gcgccagcga cgccttcctg cacgcgcagg
     9601 acacgatggg gatgacgccc gaccgcatcc cgaaattcag cgacctcaac cgcgtcctcg
     9661 ccgatgcgac aggctggcag atcgtcggcg tggagggcct gctgccggaa ctggtgttct
     9721 tcgaccacct ggccaatcgc cgcttcccgg tgagctggtg gatccgccgt cccgaccagg
     9781 tggactacat cgaggaaccc gacctcttcc acgacctgtt cgggcatgta ccgctgctga
     9841 tggacccgat gttcgccgac tacctggtgg cctacggcaa gggcggggtg aaggcgcacg
     9901 gcatcggccc cgaagcgctg atgcagctgg cgcggctgta ctggtacacg gtggagttcg
     9961 gcctgatccg ccagcccgat ggcctgcgta tctacggtgc cggcatcgtc agttccaagg
    10021 gcgaatcgat ccattcgctg gagagtgcgg cgcccaaccg gatcggcttc gacctggagc
    10081 gggtgatgcg cacccgctac cgcatcgaca gctaccagca gacctatttc gtcatcgaca
    10141 gctacgaaga gttgatcgaa gcgacgctgc ccgacttcac cccgatctac gagcgcctgc
    10201 gcgagcagga cgcgatcccg gccggcagcg tgctcggcac cgatgccgtc ttccagcgcg
    10261 gcaatggcga gggttgggcc agcgacggcg acgtctaagg cgtcgttgcc cgcctcagca
    10321 tcccgctagt gcccgctgtc cacgggcgcc gcccgcggtc cgaacgcgcg ccgggtgacg
    10381 gcgcgaatgc tcaggccctg caccaggatc gagaacacca ccactgcgta ggtcatggtc
    10441 agcagcaggt cgcgcgattc cccgcccggc agcgccagca ccaaggccac cgagataccg
    10501 ccacgcaggc cgccccaggt cagcacttgc cacgacccgg cgggcaggcg gaaccagcgc
    10561 ggcgccagcg cgaccggcac gcccaccgcg agcaggcgcg cgcccagggc gacgaagacc
    10621 gcgaccagtc ccgccagcac gtaggtgtcg tcgaaatcga ccaggacgat ctccaggcca
    10681 agcaggacga acaggacggc gttgaggatt tcgtccagca gttcccagaa caggtcgacg
    10741 tggtggcggg tggtgtcgct catcgccaat cggcgaccgt ggtgaccgac caccaggccg
    10801 acgatcacca tcgccagcgg accggacacg tgcaggcgct gggccagttc atagccgccc
    10861 agcacgccgg cgagggtcag cagcacctcg accttgtagt ggtcgacgga gcgcagcaac
    10921 aggaacatca ccgcgccgac cgccgcaccg agcagcagtc cgccaccgac ttccatcgcc
    10981 agcaggtgca tcgcgtccat gacgcccggt gcttcctggc ggaccaccag gcccaacagc
    11041 aggctgaaca ccaccacgcc cacgccgtcg ttgaacagcg attcgccgga caccaccagg
    11101 cccacgttgt cgggtgcgcc tgcggacttg aggatgccca tcaccgcgat cggatcggtg
    11161 ggcgagatca gcgcaccgaa caccaggcag tagcccagtg gcaggccaac gcccaccagc
    11221 ggcaacagca ggtacagggc aaagccgacg atcgcggtcg acaccagggt gccgaacacc
    11281 gccagcacgc cgacctgcca gcgatagctg cgcagcaggc tcacgtccac gtgcaacgcg
    11341 ccggcgaaca gcagcatcga caacatgccc tgcatcagca cgtcgctgaa atcgatcgac
    11401 cgcaggaact gcttctcgta ggcgtacagc gcgccgaagc ccagcttgct ggcgaccagc
    11461 agcgccagcg acagcagcat cgcgatcgcc atcacgccga tggtgttggg caggcggatg
    11521 aagcgcacgt tgaaccaggc cgccagcgcg gtgatcacga ggcaggcgac ggcgatgtcg
    11581 aacatgtggg cagccgttgg cacggggacg tccatttgag cagataccgc ccccgctgcg
    11641 gctatgcttt cggccatgaa ctctccagcg ctcgacctcg catcgcccgc cgcctggtgg
    11701 cgctgggccc ggcaatggcg caccacgcgg ctggtggccg ggatagatcg catcgccgtg
    11761 ctggagcatg cccatgacgc cggcgctctg gggccccgtt acgccttcat ggtcgtgatg
    11821 tcgtgcggca tcgccgccct gggtctgttg cagaactcgg cggcggtgat catcggcgcg
    11881 atgctgatct cgcccttgat gggcccgatc atcaatctcg gcatgggcct ggccacgttc
    11941 gacctgcgca ccatccgcgg gtcgctgggg acactggcgg cgggcgtcgc cctggcgctg
    12001 gcgatcgccg tggcgatcgt gtcgctgtcg ccgctgcagg aagccaccgg cgaaatcctg
    12061 gcgcgcacgc ggccgacctt cttcgacctg ctggtggcgg tcctgtccgg cctggccggt
    12121 gcgtatgcca cggtcacccg caagggcgag accatcgtcg gcgtagccat cgcgaccgcc
    12181 ctgatgccgc cactcgccgt ggtcggctac ggcctggcgg tggccaactg gaacatcgcg
    12241 ggcggcgcgg ccttcctgtt catgaccaac ctgctggcga tcgccctgtc ggtgaccatc
    12301 gtcgcccgct ggtacgggtt cggcggcgcg gacagcccca agcagacggc atggcaagcc
    12361 gcgctgatca tcgccagctt cgccctgctg tcggtgccgc tgggcctggc gctgaagcgc
    12421 atcgcgctgc agtcgcaaac cgaactgacc gtgcgctcga gcctggaagc acgtgccgcg
    12481 caggccgggg gacgggtcag cgccttgcgg gtggataccg ccgaggacag cgtggcggtg
    12541 gacgccgtga tgatgctgcc ccgccacgtc aatggcctgg aaacgcagct gcagcaacag
    12601 ctggcggcac gcaccggtcg gccggtgtcg ctgcggatac gggaggtcct cactgcggac
    12661 gatgccagct tcgcgctgca gcagagcacg ctggcggaac tgcgccgcag cgtcaccgcc
    12721 ctgcaggacg ccgaaaccgt gcgcagtgcg gcattgcggg cacgggaaga acagcaggcc
    12781 gggatgcagg cacgcctgct gccctggctg ggcaggctgg aacgcgcggc cgacggcagc
    12841 cactgggcgc tctggctggc cgatggcagt cttccgctgg cccgcgcgat gcagatggaa
    12901 agcgacatca acgccggtcg caacggcgac atgctgccgc tggtggttca tcctcccctg
    12961 caggcattgg ccgcgatccc gctgcccgcc accgacgacc ccggcgcgga ccgcatgctg
    13021 gccatccagg cctgggcctt gcgtcgctgg cgcgcgtcga gcgtgcaggc gacgttggtg
    13081 actgcggacg atgcgcaggc cgcggcatgg gaagcccgct tgcgcgaggc gctgtctgca
    13141 cgcggcatcg cgctgggcga actgcagcgc gagcgcaagg gcgaggcgcg cctgcaactg
    13201 caggcggcgc cggcaccctg aacccgcgcg gggctcagcc ggcgtggctg gccggcggca
    13261 gggcctcgcc cggcgtcagg tcgatggcgg atgccgttgc gcccgcaccg acgtcgacca
    13321 ccatcgcggt gtccggtggg cagggcgcgc ggatgcagcg gatgcgctcg gcgtgcgcaa
    13381 acttcatgcc cggatcctcc aatgcctggc cagcgacgac atagcgtccg gccgcgactt
    13441 cgatgcggta ggtcgtcgcg ccggccacgg tgtcgatgca ggtcggcgtg ccgccatcga
    13501 gcgggtgcgc gcacacccgc agcgcgggcg gcggccggtt gccctcgcgg atcgtgcctt
    13561 cgatggcgga tccggcagcc gcaacaacgg gttctgccga ggcgggcggc gtggccgcgg
    13621 atgtggcggg cggcgcgggc ggcgcctcga ccggggcggg cgccgaagcg gtcggtgccg
    13681 ggggggccgc cggggcgggt ggcggcgaac acgcggccag cgagaggagg atcacggcgg
    13741 gcagcagcaa tggacgtggc atcgggacgc tccgtggcgg acttcggcga caataacgcc
    13801 tccccttgcc gccggcctgc acatgtcgaa cacgagcatc tcgttgacgc gtttcctgat
    13861 cgaggaacag cgcgccgacc gcatcaaccc cgacctgcgg ctgctgatcg aagtggtggc
    13921 gcgtgcctgc aagaccatct cgatcgcggt cggcaagggc gcgctgggcg gcgtgctggg
    13981 cgatgtcggc atcggcagcg acgccagcat caacgtgcag ggcgaggcac agaagaagct
    14041 cgacgtactg agcaacgaga tcctgatgga agccaacgcc tggggcggcc acctggccgg
    14101 gctggcgtcg gaggaaatgg acacctcgca tccgatcccg gacaagtatc cgcgcggcaa
    14161 ctacctgctg ctgttcgatc cgctggatgg cagctcgaat atcgacgtca acgtgtcggt
    14221 ggggacgatc ttctcggtgc tgcgttgccc ggacggcgtg accaatccgg tcgatgcgga
    14281 tttcctgcag ccgggcaccg cccaggtcgc cgctggctac tgcatctacg gccccagcac
    14341 cacgctggtg ctcaccgtcg gccacgggac ccacgccttc accctggatc gcgaagtcgg
    14401 cagtttcgtg ctgaccgtgc gcgggatgcg catcccggag gagacgaagg aattcgcggt
    14461 caacatgtcc aaccagcgcc actgggagcc gccgatgcag gcctacgtgg gcgacctgct
    14521 gttgggatcg gaaggcccgc gcggcaagga cttcaacatg cgctgggtgg cgagcatggt
    14581 cgccgacgtg caccgcatcc tcacccgcgg tggcatcttc agctacccgc tggaccgcaa
    14641 gtgcgcggac aagggcggcc gcctgcggct gatgtacgag gccaacccga tgtcgttcct
    14701 cgtcgaacag gccggcggcg cggccagtac cggccgccag cgcctgctgg aagtccagcc
    14761 gaccggcctg cacatgcgcg taccggtgtt cctgggatcg aaggccgagg tcgaagccgc
    14821 ggtgggctat cacgccgcgc gctgacaccg ctcagccgcg gccgggcgcc aggttgagct
    14881 cgagccagta acggccgatc tggcgcaccg cctcgacgaa gcgttcgaat tcgaccggct
    14941 tctggatgta gctgttcgcg ccgagcgcat aggcctggtc gacgtcgcgc ggttccgcgc
    15001 tggtcgtgag caccaccacc tgcagggccc tcgtcgccgg atcggcccgg atcgcctgca
    15061 gcacctcgcg accatcgagg cgcggcaagt tgaggtcgag caggaccagg gcgggcaggt
    15121 ccgcgccgtc gcggtccgca tggcgccccc gcgccagcag gtaatccagc gcctccgcgc
    15181 catcgccgac ggcatgcagg cgatgcgctc ccccggcctc ggcgaacgcc aggcgggtca
    15241 gctcgacatc gtccgggttg tcctcgacca gcaggatgtg gtgggcgctc attgctcgcc
    15301 tcccgggatc ggcacgtccc gcaggtggac gtggaacgtc gcgccttcgc cggggatcgc
    15361 ttcggcgcgg atgtggccgc cgtgccgcgc aacgatcgca tcggcgatgg ccaggccgat
    15421 gccgtcacct gcgccctcgt cgctgccgtg caggcgctgg aatggctcga gtatccgcgc
    15481 ggcatacgtc atgtcgaagc cgacaccgtg gtcgcgcacg gccaggtcca ggccactgtc
    15541 gttgcgggtg ccggtgacgg cgatctgcac ctgcggccgg ctggccgaga accgccaggc
    15601 attgcccagc agctgcgcca gcagggtctt gagcaggcgt tcgtcgccga cgacctccag
    15661 tccttgctgg acgtcgatga ccgcctcgcg gccgggatgg gcatcctgca gttcggacgc
    15721 gcaccagtcg gccagcatgc tcatgtcgac gcgcatcggc cgcaattccg cactgccggc
    15781 gcgggccagt tcgaccaggc cgtccatcag cttggccatc ctcgcgctgg tcgcgctgat
    15841 ccgcgccagc gattcgatcg ccgcgggatc gcccgccgat tccagttgcc gcgacaaccg
    15901 cgacgcgaat ccatcgatcg cccgcagcgg cgcgcgcagg tcgtgggaga tgccgtgcag
    15961 cagcgcctgc aggcgcttgt ccgcggcgtc caggcgtgcc tgtagctgcg cttcccggtc
    16021 catgttcatg cggcgtggct tcccggggaa ccgccacgcg cgcccggaca cgcgccggcc
    16081 agcctcgcca gccggcagcg tggggtgtcg cgggatgtcg tgggcagcga aggcatgggc
    16141 gtggttcttg tcggcgcagg gtagcgcctg cctgcgtaaa cgcagtgtgc aaaccgcggt
    16201 ggcggcgtcc cgcaggcgaa caggcagaat gggggcatgg aattgccacc gcacctgttc
    16261 cagcaagcat cgttcgggtt gatcctggtc ggcgggctgt acctgttcat cagcgaaaag
    16321 ctgcgggtcg acatcaccgc gatgctgatc ctgctggcgc tggtgctgac cggggtactg
    16381 gacagcaagc aggctctgtc cggcttcgcc agcgaaccgg cgatcatcgt cgccgcggtc
    16441 ttcgtgatct ccggtgcgct ggcggcgacc gggatcagcg aacgcctggg cacctgggtg
    16501 agccgggccg gcgggcgcgg cgaggcccgc acgatcgcgg tggtgatgcc gctggtggcg
    16561 ctgctggccg ccttcaccca ccacgtgatg gtcaccgcga tgatgctgcc gctgctgctg
    16621 cggcacgcgc gcgaccgcca gctgccggcc tcgcgcctgc tgatgccgat gtcgctggcc
    16681 gcgtcgctgg gcaccaccct gaccctggtc agtgcgccgg ccttcctgct cgcgaacgac
    16741 ctgctgcagc gcgcaggcgc agaaagcctc ggcatcttct cgatcacccc gatcggcatc
    16801 gccctggtgg tggtcggcac cctgtacatg ctggtcgcgc gctggctgct gccgcggcgc
    16861 agcggcgagc gcaacgacga ggactacctg cggctggacc gctaccgcac cgaactgctg
    16921 atcgtgaagg acggccgctg gtgcacgcgg ccgatgtcgg aactgcagaa ggcgctgggc
    16981 cagcgcttcc gcatgctcgg ctggctgcgc gatggccggc gccgcgacga cctgaccgcc
    17041 gccagcccgt tgctggccgg cgacatcctg ctggtcgaag ccgccgccga cgaattgctg
    17101 tccctgcacg aggacgccgg cctggacctc aacgcgatcg cccgcttcgg cgaccagctg
    17161 gaaggcgaag gcgatccgca gctggtccag gcggtggtcg cgcccggctc cgagttcatc
    17221 ggccgcagca tccgcgaact ggatttctcg cggcagttcc acgcggtcat cgccggcctg
    17281 tggcgacgtg gcgcggacat ggccgaccgg ctgtccgatg cnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn ncgcgggatc gacgtgcgca tcttcgtgat gatcgccggc
    17461 gtcatcccgc tcggcatcgc catggagcag accggcaccg ccgacctgct ggcacagggc
    17521 ctgtcgcacc tggtcgcgca gtggccgcca ctgctgatcc tgctggtcat gttcagcgtg
    17581 gccgcgctgc tgacccaggt gatgagcgac gccgccacca ccgcgttgct gggtccggtc
    17641 gcgatcgccc tggcgcaatt gctggacctg ccgccgacgc cgttcgtggt gtgcacggcg
    17701 ctgggcgcgg tggtcgcctt cctcaccccg atcggccacc acggcaacct gctgatcctc
    17761 gggcccgggc aataccggtt cggcgatttc ctgcgcatcg gcctgccgct gaccgtggcg
    17821 atcgccctgg tcagtgcgtg gatggcacgc tggctgtggc tcgacgggcc gctgtggccg
    17881 accttgcaat gaccttcgga gtttcgcgat gacgacactg attgccccgc gcgtgcacga
    17941 tctcggcggc ttccaggtgc gccgcgcggt gccctcgctg caggcgcgca gcgtcggccc
    18001 cttcgtgttc gtcgaccaca tggggcccgc ccatttcgat gccggacacg gcgtggacgt
    18061 gcgcccgcat ccgcacatcg gcctggccac ggtgacgttc ctgtggtcgg gcgggatgcg
    18121 ccatcgcgac accctcggca gcctgcagga catcctgccc ggggacgtga actggatgac
    18181 cgccggccgc ggcatcgccc attcggaacg cacgccgccg gcactgcgcg aaaccggctt
    18241 cccgctgcat ggcatgcaga cctgggtggc gctgccgcag tcccaccagg aggtcgaacc
    18301 atccttccat caccacgccg cggcctcgct gccggcacgc gatcatgaag gtgcgcgctt
    18361 gcgggtgatc gccgggcgcg ccttcggcat ggaatcgccg gtcaaggtgt tcgccgacac
    18421 gtggaacgtc gccgtcgacc tcatcgccga tgccgaactc gtcattccgg cggatgcggt
    18481 ggaacgcgcg gtttacgtgc tcgacggcga ggcgcaactc gacggcaccg acatccccga
    18541 caagcacctg ctggtgctcg accgcggcgt gcgccacgtg ctgcgcgcga agacgccagt
    18601 gaaggcgatg ctgctcggcg gcgaaccgct ggatggcccg cgccatatgt ggtggaactt
    18661 cgtgtcgtcc tcgaaggagc gcatcgagca agcgaagcac gactgggacg aaggccgctt
    18721 cgggttgatc ccgggcgacg atgtggaacg gattccgctg ccggagcgtt gaggcagcat
    18781 cgcggggcca gcgcagcagt cccgataccg ggaattgctc ctcggcttct gcgaacatcc
    18841 tgttcgaggc gccgccgcgc gccgtccctg gcgcgctttc cgcaatcccc ggcatcgggc
    18901 ctgccgacgc attcctgcga cgtgaggtca gaacaaggag ccctgcggcg agggcttccc
    18961 cggcgccacg aacaggtcgg tgcgcaacgg cggcacgccc tcgaacccca gccgcttgcg
    19021 ggtcttggca aagcgcatcg cgacaaggtc cgcgaacggc ccctgcccgc gcatgcggct
    19081 gccgaacgtg ctgtcgtaat ccttgccgcc gcgcatctgc cggaccaggc tcatcacgtg
    19141 cccggcgcgc tccgggtagt gcagctgcaa ccactcgcgc cagacctctt tcaactcatg
    19201 cggcaggcgc agcagcacgt agccggcgga ctgtgcgcca gcgtcgcggg cggcttccag
    19261 gatctgctcc aggtgcatgt cggtgatcat cgggatcacc ggcgccacca gcacgccggt
    19321 gggcaccccg gcgtcgcgca atgcggacag catcttcaat cgcgtgtgcg gcgctgccgc
    19381 gcgtggttcc agtttcgacg acagcgtgtt gtccagcgag gtgatcgaca cgaacacgct
    19441 caccaacccg tgtttcgcca gttcggcgag caggtcgagg tcgcgcagga gtgcgctgcc
    19501 cttggtgacg atgctcagcg gatgccgcgt ctccagcaag acttccaggc aggcccggct
    19561 gatcccgtgg cggcgctcga tcggctgcca gccatcggta ttgatgccca gcgcgatcgg
    19621 ggcggggacg tagccaggct tcgcgagttc cgcgcgcagc aattccggcg cattggtttt
    19681 cgcgaacaac cgtgtttcga agtcgagccc gggcgacagg tcgaggtacg cgtgcgaggg
    19741 ccgcgcgaag caatacacgc agccgtgttc gcagccacga tacggattga ccgattgcga
    19801 aaacgcgatg tccggcgact ggttgcggct gatgatgctg cgcgcccgtt cgacggtcac
    19861 ctgcgtgcgc ggcgacggcg catcgtccat gccgtcgtac accgaacccc agccatcatc
    19921 ttcgccgcgc acctcgcgcg tctcgaaacg cccggccacc cgcgacgccg cgccgcggcc
    19981 cctggtgggc gccatcgcgc ggcgggcatc gtcgccgggc cggccctcgt cacgtgaaat
    20041 cgccatcgtt ccaccctacc ctgtccacgt cgcaacgccc gcgacgatgg agccccgatg
    20101 cccgacctgc tgcatgcctg ggagaccttc tgggcgatcc cgcacatccg cgcctggctg
    20161 accgccggct ggatcctcta cctggtgtgg ctggggttgt ggatcgtgct gcagaagcgc
    20221 gagcccgcgg cgaccctgag ctggctggtc agcctggccg cgctaccgta catcggcttc
    20281 gccatctatt acctgctggg cccgcagaag atcacccgcc atcgcgcgcg ccggttgcgc
    20341 catcgcgcct cgctggcgcg cgagccggaa gggccgggcg acgacagcgt ggaactgcgc
    20401 acgctggccc acgccaccac cggactggcc ccgaccaccg cccgcgaggt gcgcctgctg
    20461 gtcgatggcg gcagcaagta cccggccctg ctggacgcga tcgccgcggc ccgcgaccac
    20521 atccatctcg agtactacat ctattgcccg gaccggaccg gcacggccct gcgcgacgcc
    20581 ctggtcgagc gcgcccgcgc cggggtcaag gtgcgcctgc tgctggatgc gatgggttcc
    20641 aagcaggtca agccgcgctt cttcgcaccg ctggtggagg ccggcggcga actggcgtgg
    20701 ttccacccgg cgcagccatg gacgctgtgg aagcggccgt gggtgaacct gcgtacgcat
    20761 cgcaagatcg tggtgatcga cggcgccgtg ggtttcctgg gcggcatcaa catcaccgac
    20821 gaggaagacg aacgcctgcg cgaggatgcc taccgcgacc tgcacctgcg ggtcgagggc
    20881 gacgtggtcc gcgcgctgca ggaggtcttc gtcgaggact gggcgtacgc caccgagtcc
    20941 cgcgacttcg tcggcgacgt ggcccgggcg atgccggcgc cctcgccggg gccgatccgc
    21001 gcgcagctgc tgagttcggg accggattcg ccatgggagg cgatccaccg cctgcatgtc
    21061 ggtgcgatcc actcggccgg caagcgggtg tggctggcca cgccctactt cgtccccggc
    21121 gaggcggcga tgatggcgct gacctcggcc gcgctgggtg gcctggacgt gcgcctgctg
    21181 gtaccgaaga tgagcgacag ccggctggtg acctggtgcg tgcgctccta cttcgacgac
    21241 ctgctggcgg cgggggtcaa ggtgtacgag tacggcccgc ggatgctgca taccaaggcg
    21301 ctcctggtcg acgaccagct ggccatcatc ggcaccgcca acttcgacca ccgcagcttc
    21361 cgcctgaact tcgaggcctc gttgctgttc gaggacgccg gcgtggccgc cgagctggcg
    21421 caggtcatcg aacgcgactt cgcctccgca ccacgcgtgc gcgacgaccg cgcgcgtccg
    21481 ctgctgggcg cacgcctgcc cgaggcactg gcgcggttga tcgccccgct gctctagcgc
    21541 cggcgcttgt cgtcgcggtg accgctggcg gtaaactggg tccttcccgc ccggagcgcg
    21601 cgcatgcact ggctgttctt gctgatcggc ttcgccctgc tggtgctgtc gttcctgacc
    21661 accaactcgg tcgtgatggt gctgtgctgg ctggtggcgc tggtgctgtt cggcctgtgg
    21721 ctggcggcga tgtattccgc acgggtgacc agccaggagc gcgacgtctc gatgatgatc
    21781 gaccccgccg agctgcgccg cctgcgcgaa caggccgaag cccgcaaggc cgcggccagc
    21841 caggacaccc cggattgatc acgggcccgc gcctagtcgc tccgcacgca tgactgcgct
    21901 cagcgtcaac ctcaacaaga tcgcggtgct gcgcaattcg cgcggcggcg ccgaacccga
    21961 cgtgttgcgc gcggggatcg cctgcctgga tgccggcgcg catggcctga ccgtgcaccc
    22021 tcgccccgac gcccgccaca tccgcgaaga cgacgtgcat gcgctcgccg gactgtgccg
    22081 gtcgcgcggc gtcgagttca acgtggaggg caatccgttc gccccgccgc gtcccgggta
    22141 tcccggcttg ctggcactgt gcgacgcagt gcgaccggcc caggtcaccc tggtgccgga
    22201 cggcgatggc cagctgacct ccgatcacgg cttcgacttc accagcgatg gcgacgcgtt
    22261 gcgccagttg gtcgccgcct acaaaaccct gggatgccgg gtcagcgtgt tcgtcgatgt
    22321 cggcgacccg ctggtcgccc gcgccgcgag cgccggtgcc gaccgcgtcg agctctacac
    22381 cggcccattc gcagaggcgt tcgcccgcgg cgatgccgat gcggcgttgc gcatggcggc
    22441 ggacacggcg cggctcgcgc aggcggccgg gttgggcgtc aatgccgggc acgacctcag
    22501 ccaggcgaac ctggcggagt tcctcgcgcg ggtccccggc gtgcaggaag tctcgatcgg
    22561 ccacgccctc atcagcgaag ccctctacgc cggcttggac gcgacggtcc gcgcctaccg
    22621 ccacatcgtc gatgcggcgg tagctggccg ggtcaacgcc tgatcgcgat gtcccggtaa
    22681 cccgcgttgc gggcggcggc cagggcgaac gcgaacgccc ggtactcggc atcgccactg
    22741 ccatccagtg acaacccggc cttcggcgcc tgcgcatgct gtgcgcgcag ttccgccgca
    22801 agcgccggca gttcgacgcg cacgccgtcc agcaatgcgc tgccgtcggc ctgcacctgc
    22861 agttgcaccg gttcgggtgg cgggatcgcc cgtggcggcg tgattcccgg cagggtcaag
    22921 tcgatcgatt cgctgcggat cggcatggtg accatgaaga tcaccagcag gaccagcatc
    22981 acgtcgacca gcggcgtgat gttgatgcca gccatcgcag cggcttgtgg ctgcggtcga
    23041 gcaaacacgg aagcggtcat gcgacacctc ctgcgggcct gatgcccgac tccacgctgc
    23101 acccgccgcg tcgtgccgtc aagacccgtt caagcaacat cccgtcatcg agccgagcgg
    23161 tgcctgtacg cccgaggcat aaaaaaacgc cgcccttgca ggcggcgtcg tcatccacga
    23221 cgtgctccgg agtccggagc agggaattgt tcggaactca gttctggacg aagccgatct
    23281 tgaccatttc cgcattcttg gccgaggcca ggaccttcgc cagcacgcca tagtcggcgt
    23341 catcgctggt gtcgatctcc agcgtcggct ggttggtcgg atcgcgttcg acttcggtct
    23401 tcatcatcgt ctgcaatgcg ttcaccggtg tcggcgtgtt gttccagaac acctggccgg
    23461 cagcatcgat gcgcaagctg atcggatccg gcggttcctt cggctgcggc ggcggattga
    23521 gcgtcttctg cggcaggtcg acatcgaccg gcaccgactg gatcggcatg gtcaccatga
    23581 agatgatcag caacaccagc atcacgtcca ccaggggagt gacgttgatg tcggcgagtg
    23641 ggccggaacc gccgctggaa ctgaatgcca tgactgtcta cctcagatgt tcttcttcgg
    23701 cggggtcgtc acgaacccga ccttgcgcat gccctgggcc tgggccacct tcaccacgtc
    23761 gttgaccaac cggtacttgg tggtcttgtc ggcgcggatg ttgacgaccg gctgcggatc
    23821 cttctgcgcc tcgaccgaga gcttgctctc gatcagctgg cgggtcgacg gttcgtcgtt
    23881 ccagaacagc tcgcctttct cggtcaccgc gatggtgacc ggcggggtct tcagccccgc
    23941 cttttcgtca ggcttcttgt ccagcaccgc ttccggcagc tgcaccttga ccttatggtt
    24001 catcagcgga gtggtgatca tgaagatgat cagcagcacc aacatcacgt ccacgagggg
    24061 cgtgacgttg atttcagcca tcgggccgcc gctgtcgtta ccggaactga aggccatcgt
    24121 ggtgctccgt caggacgcgc gcgagatcaa cgcttgccgg caatttcgcc gacgcgcgag
    24181 ccggtggcga agaagtcgtg caggtcgtgc gcgaaggtgt cgaactggct gttggtcacg
    24241 cggttcaggc gcacgaagaa gttgtacgca agcaccgccg ggatcgccac gaacagaccg
    24301 atcgcggtca tgatcagggc ttcacccacc ggaccggcca ccgccgagat ggaggcgtcg
    24361 cccgaggcac cgatcttgat cagcgcgcgg tagatacccc aaacggtacc gagcaggccg
    24421 acgaacggcg cggtcgaacc gacggtggcg agcacggtca ggccgttctc gaggcccgag
    24481 gactcgcggg tcacggcctg gcgcagcgcg cgatcgacga actcggagcg gttcagcgac
    24541 tcggcgaggc gcgaaccatc gtggcgctgg tggtgcgcgg cggcctgggc ggcgtcgagg
    24601 gcgatcttgg agaacggctc ggacttcggc tgttcttcca tgaagcggat cgcgtcctgc
    24661 gcgttcgggg tttcccagaa cgtgccgacc acgttgtcgc cacgcccgcg caggcggatg
    24721 ttcttgacga agttgatgat gatccaatac cacgacatca gcgacatcac catcaaggtg
    24781 acgaagacga tccagccgac ggcgtcgagc tgggtgatga ggtgatcgaa gcccatctgc
    24841 tgaagcgcgg cggcgttgtt gcctccggcg gcggcaggtg cggcagcagt cgtttcttgc
    24901 aacatacgct tacctttgga tgtcgtggat ggggttgtgc gaaagggaca acgtcaacgc
    24961 tggaacgggt ggaacctgga aatcagttga gcttgaattc gaccgggacg cgaacgcggc
    25021 tggcgatctt cttgccgtcg cggacttccg ggttgaaacg ccagcgccgc gccgccttga
    25081 ccgcctcgcg gtcgaggttg cggttgccgc tggaacgctc cacctcgaca tccagcacgc
    25141 cgccactggc gtcgatgctg acgatgagga cggttgtgcc ctggatgttg cggcgctgct
    25201 cttcacgcgg gtatgcaggc ggattcatcg cgcgcgacga ggcatccacg ctggagccga
    25261 tgtccggaac cgagctgggc ggcgcgggcg gcgacggcgg cggggcctgc acgtccatcg
    25321 ggctggggtc gttgaacacg accggcggat cttccggcgg cggcggcacc gggctggcgc
    25381 gcggcggcga cagcgtcttc ggcggggtca gcggcttggg ctggtccggc ggcggcggcg
    25441 gcggcggcgg cgggggtggc gggggctcga tgaaggtgac gttgaccaca tcctcttcct
    25501 cgacgtcctg ggccggcggc gaaatcggcg caagcaacag cagcagcgcc gccgcatgca
    25561 gggcgatcgc gaagctgatc cccatgatgc gcggccagct caaaccgcct tcgcggtcgt
    25621 cattcttctc ggtgttggcg agatcttgcg tcatgcggac ggctctggat gtgggaaacc
    25681 ccggcctggt cgaccggggc gacaacgtgc ggcacccggg ccgcaggaac cacctagtct
    25741 ataccaatca cttgcgcttg ttccaagccc cggcgatggt gcaggtggat ttctgcgcac
    25801 cgcgtctcag cgcgagagga tgccctttgc ttcttccggc ttcttgacgc ccttgtccag
    25861 tgccttttgc gccgccgcct tggcttcggc tttcttgcca ctgtactcga ggaccttcgc
    25921 gaggttgagg taggcctcgc catccgcggc catcggcgcg gccttgccgt acaggtcgat
    25981 cgccagtgga atcttgtcgt cggcataggc gttctgggcg aggatctggt aatcgcgggc
    26041 caggtccggg ccaggctgca gcacgccctt gctcacgccg gcgtcgatca ccttctgggt
    26101 gtcgtcccag cgcgaggcat tcatgtagcc gatgtacagc gcccgcaact cgcgactctc
    26161 ggtcagcatg ccgcgcttgt aggcgtcctc gagcagggcg ttggcctggt cctgcttgtc
    26221 cgcgttctgc agcgccgcca cggcattcat caggatccgc ttgtcgttcg ggttcttcgc
    26281 gatgaggtcc ttgtagatcg cagcggcctc gtcatgacgt tccaggttgg ccaggatgcc
    26341 ggcgcggaac gactggtgct cgggcttctc cgacttcgat tcggccagga aacgatccac
    26401 ggtcgccagc gcttcggcgt atttctccag gccgaactgg atcaccgcca ggttgaacat
    26461 ggtggtgtag tggctgtcgt tgtcgaggcc gttcgcgtcc acggccctgc ggaaatactc
    26521 ggccgccttg gcctggtcgt cctggtcggc ggcggcgttg gcggcgagcg aatacgcgaa
    26581 cgacttgtcg taggcgttcg ccgacggcat cgccacgatt tcttcggccc gtgcgatcac
    26641 cgcggtcatg tcccgctttt cgaacaattc ctgcaaaccc tgcaggttct tgatcatctt
    26701 gccgctggac ttggcgtcgg gctgtgtgcg ggtcgcgttc ggataggcgg ccggcttgtc
    26761 ctcggcctgc ttggcctctt ccttgccctt gcccagttcc gccatgcgtt cggcgcgttg
    26821 ttggcgcatg gtcttggtgc cggtcgtcgc ggtcgggtcc tgctgtgcgt tcgcggcggg
    26881 cgccagcagg gcaagtgcga gcgcagcggc aatggacgtg gcgagtgggc ggttgaaagt
    26941 cggcttcatg gcaaacctcg aacgagaaag gggctgcggg atgcggcagg ggtcgcaccg
    27001 tagcagaaag tccggcactt gaaccgtgcc ggaatgatgc acctagtgcg cgttaaatca
    27061 tcggcaacgc ggggccttgc cggcgctgcg cgcagcctgc atggtcggca acaacgcgtc
    27121 tgcacgggac tccagctcac gcaggcgcga ggccggatcg gggtgggtgg agagccattc
    27181 cggggaacgc gcaccacccg ccgccgacat gttccgccac aacgccaccg caccgcgcgg
    27241 gtcgaagccg gcgcgggcca tgatgtcctg gccgaccacg tcggcctcgg tttcctgcgc
    27301 gcgcgaattc ggcagcagga atccggcctg ggcgagcacg ctgccgccct ccgtggccag
    27361 ctggccgtag tcgccggcca gcgcgccgag caactggacg gcgccggccg cgccgagctg
    27421 gcgggtgatc cgctcgtcgt ggtggcgcgc aatcacgtgg ccgatctcgt gcgcgaccac
    27481 cgccgccagc tggtcctggt tggtggcgac ctggaagatc ccggtgtaca cgccgacctt
    27541 gccaccgggc aacgcccacg cgttggcttc gctgttctcg aacagcgcgt aatcccagcg
    27601 ttgctggttc cacggcgcgg gcagctgccg tgcgaccgcg tcgaccacgc agcccacgta
    27661 ggcgacctgg cgagcatccc gcgactgcgg ggtcttttgc ttggcctccg cgaaggcctg
    27721 ctcgcccagc cggttcaact gcgcctgcga gacgccgccg acgtactggg tgcggccggt
    27781 cggcgaggtg gtggtcgcgc aggcggccac cagggcggtg gccagcaacc cggccaggaa
    27841 catcttcgtc ttcattgcct tcgattccct tgggagacgc ctgttgtagg caggcgcacc
    27901 tgaaagtgac gtcaaggcgg caatgtcggc gatcagacgt cgaggttgga caccttcagc
    27961 gcgttttcct cgatgaactc gcgacgcggc tcgaccacgt cgcccatcag ggtgctgaag
    28021 atggcgtcgg cggccaccgc gtcctcgatc ctgacctgca acaggcggcg ggtatccggg
    28081 ttcaccgtgg tgtcccacag ttgttccggg ttcatttcgc ccaggccctt gaagcgctgg
    28141 atctggcggc ccttcttggc ctcttccagc aaccaggcct gcgcctcggc gaaactggcg
    28201 atcgcctgcg cgcggctgcc ccgcacgatc tgcgcgcccc cgcgcaccag gccgtgcagc
    28261 actgccgcgg cttcgcgcag cggcttgagc tcgccgtgct cgaaggtcga cagcggcagc
    28321 acctgggtca gttcctcgcc catgtggcgg cggatgacca gcagcgcggc gggacgcgtt
    28381 tcgttggcgg gttgcaactg cagcgcatag cgaggcttgc ccaggccgcc gcggttgagc
    28441 cttgcggcca gcgcgtcgag ttcgcgggcg tcgtcggtgt gcgcagccag cgaggcggca
    28501 tccagcgggg tgaagtcgat cagcgactcc agcacgatcg ggtcgaagcg atgcgcgttg
    28561 cgggcgatcg cttcgcgcgc gcgcgcgaac accagcagca acgattccag cgcagcgcct
    28621 tcgatcgccg gttcgttcgc cgccggcacc agttgcgcgc cctccacggc gttgccggcc
    28681 aggtacgcgt cgagcgccgc atcgtccttc aagtacagct cgttcttgcc ctgcttgatc
    28741 ttgtacagcg gcggcaggcc gatgtagatg tggccgcgct cgatcagctc cggcatctgc
    28801 cggtagaaga aggtcagcag cagggtgcgg atgtggctgc cgtccacgtc ggcgtcggtc
    28861 atcaggatga tgcggtggta gcgcagcttg tccgggttgt actcgtcctt gccgatgccg
    28921 gtgcccagcg cggtgatcag cgtgcccacc tcggcgctgg ccagcatgcg gtcgaagcgg
    28981 gcgcgttcga cgttgaggat cttgcccttc agcggcagga tcgcctgggt cttgcggttg
    29041 cgaccctgct tggcggagcc accggcggag tcgccctcga cgatgaacag ttccgacagc
    29101 gccggatcct tctcctggca gtcggccagc ttgcccggca ggccggcgat gtccagcgcg
    29161 cccttgcgac gggtcaggtc gcgcgccttg cgcgcggctt cacgggcacg cgcggcatcg
    29221 acgatcttgc cggcgatcgc cctcgcctcg ttggggtttt cctgcaggaa ttcctcgagc
    29281 ttggcggcga acacggcatc gaccaccgcg cgcaccggct cggtgaccag cttttccttg
    29341 gtctgcgagg agaagccagg atccggcacc ttcaccgaca gcaccgcgat catgccttcg
    29401 cgcatgtcgt cgccggacat cgacaccttg gcctgcttgg cgatgccgct ctgctcgatg
    29461 tagcgggtca gggtgcgggt gagcgcggcg cggaagccgg tcaggtgggt accgccgtct
    29521 ttctgcggaa tgttgttggt gaagcagtac atcgtctcct gatacgaatc gctccactgc
    29581 acggcacaat cgacgacgat gccgtcctgt tcgccggaga tcgagatcac attggggtgc
    29641 agcggggtct tcagctgggc caggtgctcg acgaagctgc ggatgccgcc ctcatactcg
    29701 aatacgtcgt gcttgccatc cgggcgctca tcgaccaatt cgatcttgac gccggagttg
    29761 aggaaggaca gttcgcgcag gcggcgcgcc aggatgtcgt agtggaattc gacgttgtcg
    29821 ttgaacgccg tcaccgaagg ccagaagcgg acttcggtac cgcgcctgtc cgacgcctcg
    29881 agttgcttca gcggatacac cggctcgccg tggcgatatt cctgctggta gtggtagccg
    29941 tcgcgccaga tgtcgaggag gagtttctcg gacaacgcgt tgaccacgga cacgcccacg
    30001 ccatgcaggc caccggagac cttgtagctg ttgtcgtcga acttgccgcc ggcgtgcagc
    30061 acggtcatca ccacctcggc ggcggagatg tcgcgaccct gcttttcgct ttccttggca
    30121 tgcctgccca ccgggatgcc gcggccgttg tcccacaccg acaccgagcc atcgtcatgg
    30181 atggtcacca gcaccgcgtc ggcatggccg gccagcgcct cgtccacgcc gttgtcgacg
    30241 acctcgaaca ccatgtggtg caggccggag ccgtcgttga cgttgccgat gtacatgccc
    30301 gggcgcttgc ggacggcttc cagcccttcc agcgcggtga tgctgctggc gtcgtaggtc
    30361 ccgctggtcg cgggcttggt cggcgggctg gattcgggca cttggcccgg ggtggactcg
    30421 ttgttcgaca tcgacgcgtt cgtctcccgc ggcgcgccgg aaacgcgccg tgacacatta
    30481 actataggaa ccgtgagtat aacaatcgcg ccaccacgac gtccgaacga cgtcaggcga
    30541 ggcgtcccac cacgccatgt tccacgtgga acctctcgca ggggatgtcc agcgcagcca
    30601 gtgcgggaac gggatcggtg ccggtcacga agacctgggc accactgccg gcgaggacct
    30661 gcagcacgcg ctgttggtga tggcgatcca gctccgacgc caggtcatcc agctgcagca
    30721 ccggccaatg ccccgcatgc gccgccagct gcgaagcctg ggccagcagc aggaccaatg
    30781 ccgcgagctt cgtttgcccg cgcgacaagc catcgcgtcc cggcaggtcg cggaactcga
    30841 gcctgaggtc ggcgcgatgc ggacccaccg tggtgtgccc ctgcgcgatg tcacgctcac
    30901 gggcgagcag gagggcatcg gcgagaccca actcctggcg tcgccagcct ggctgcaggg
    30961 acaggctcaa accgcccgct gccggcagga tttccgcgat caaggcgtcg acatgcggct
    31021 gcaagcgcgc cacgtaggcc tcgcgctgca gggtgatcgc ctcgccggcc tgcgccagct
    31081 cgtgttccca cgcatccagc tgggcgtctg cccgcccctg ccgcagcaag gcattgcgct
    31141 gcttgtgggc tcgcgcgtgg cgacgccaga gcgaaaagaa atcgccaccc gcatcgctgc
    31201 gttccacgtg gaacaacccc cagtcgaggt agcgccggcg cccctcgctg ctgccgtcga
    31261 ccagcgcgtg gctgccgggt tcgaaggtga tgactgccaa cgcttcgcac agttcaccca
    31321 gttggctgac ctggcatccg tccaggcggg cctcccatgc gctgccggaa tgccgcaggc
    31381 ccgctcgccg gcccggttgt cccggttgtt cccactccag gaacacctgc agcgccggtt
    31441 caccgctgcg aaccagcccg tcgcggatcc ggccacggaa actgcgccca tgcgccatca
    31501 ggtgcatggc ctcgagcagg ctggtcttgc cgctgccgtt gtcgccggtg accaggttga
    31561 acccggggcc cggccgcatt tcgacatcga ccagccgccg caggccttgg acatgcagtt
    31621 tccggatcag cacgggatgg ccctgggcgc gcgcaaacga cgacgcccgg actgggccgg
    31681 gcgtcgtctg cagtgttcca cgtggaacac gggctgtcgc agcgggagat ccggcatccg
    31741 gtcagagccg cagcggcatc accacgtggc gggcgtgctg gtgttccgcg ccacggacga
    31801 gcgcggagga attggcatca cgcagcagca gcaggatgtg ttcatcgcgc agcgcgccga
    31861 gcgcatccag caggtaattg acgttgaagc cgatggccag gccatcgacg cgggtatccg
    31921 cctcgagctc ttcctgcgct tcttcctgct cggggttgtg cgcgctgatc ttgagcaggc
    31981 cgggggacac ctcgacgcgc acgccgcggt acttctcgtt ggagaggatc gaggcgcgtt
    32041 gcagggccgc tcgcagggct tcacggtcga ccttgacctc acggtccgcg ccgatcggga
    32101 tgactgcctc gtagtccggg aacttgccat cgatcagctt gctggtgaag gtcacgtcgt
    32161 cgcgcttgac ccggatgtgg cccttgccga gttccaggtc gagcgtgcgg tcgccgcctt
    32221 cgagcaggcg ctgaagctcc tgcacgccct tgcgcgggac gatgatctgg cgcttgctgc
    32281 cgctgccgcc gtccagcgcg gtttcgcaca aggcaaggcg atggccgtcg gtggcgacgc
    32341 agcggagggt ggtgtcgcgc aggtcgaaca gcaggccgtt gaggtagtag cgcacgtcct
    32401 gctgggccat cgcgaacgcg gtacgttcga tcagttcctt caggccggcc tcgggcacgt
    32461 tgacccgttc ggtggcttcg aactcgtcga tcgaggggaa atcgttcgcc ggcaggctgg
    32521 ccaggctgaa ccggctgcgt cccgcctgca cggtgatctt gtcgccttgc tgggtgacgg
    32581 tgaccttgct gccgtcgggc agggcgcgca cgatctcgaa cagcttgcgc gccgggatgg
    32641 tggtttcacc gtcctgggca tcgtcgaccg cggtacgcgc gaccatctcg acctcgaggt
    32701 cggtaccggt cagcgacaac tggccctcgc gcaccttgac cagcaggttg gccagcaccg
    32761 gcagggtctg ccgacgctcg accacgttga ccacctgcgc caacggcttg agcaggacct
    32821 cgcgttgcag ggaaaaccgc atgtgaaccc cttgcccctt tgttggttct gggacggccc
    32881 gatggctcgg aaccgctctt gaagatctat ggataaaact tgaaagcagg tggtgctggt
    32941 agagcgaaaa tcggtgggta accggtgcaa gttattgaat aatttcggaa ttattcaatt
    33001 ttgaatggcg ggaaaaacaa acctgaagtc cgggaaaagc tgtggataac catggcgggc
    33061 caagtacggg atcgggttat ccacaacttg tcccgtcgtc acccaccggg tttgcggcca
    33121 ccgcttactc actgagtttg cggatcaact tgtcccagtc ctcgtgcagc ttgccgtcgc
    33181 tttccatcag gtggcggatc tgccggcagg catgcagtac ggtcgtgtgg tcgcgaccgt
    33241 cgaaggccac gccgatttcc ggcaggctgt gttcggtcag ttccttggcc aacgccatcg
    33301 cgacctgcct cggacgcgcc agcgaacggg tccgcttctt gccgagcatc tccctcaatt
    33361 gcaacccgta gtagtctgcc acggccttct ggatgttggc gatgctgatc gcctgctgct
    33421 gcgcccgcca caggtcgcgc agcgtttcct gggcgaattc catggtgacc ggacggccga
    33481 gcaggccggc ctgggcggtc agcttgttga tggcgccctc caactcacgc acgttgctgc
    33541 gcatcttctt ggcgatcagc tgggcgacgt cgtccgggac cagtgcgccg cgttcgcgcg
    33601 ccttgctcag cacgatctgc gcccgggtct cgaaatccgg cgggtcgatc gccaccgaca
    33661 ggccccagcc cagccgcgac ttcagccgtg gctccagccc gtcgacctcg cgcgggtagc
    33721 ggtcgcaggt caggatgatc tgctggcggc cgtcgaacag tgcgttgaag gtgtggaaga
    33781 actcctcctg ggtgcggtcc ttgccggcga agaactggat gtcgtcgatc agcaacgcgt
    33841 cgatctgctg gaactgccgc ttgaactggt cggcggtctt ctcctgcagg gcccggaaga
    33901 aggcgttgta gaactcgttg gagcgcaggt agaggatccg cgcggccggg ttgatccggc
    33961 gcatctcgtt gccgg
//
LOCUS       DAOOCX010000009        33957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_960_length_33957_cov_9.775942, whole genome shotgun sequence.
ACCESSION   DAOOCX010000009 DAOOCX010000000
VERSION     DAOOCX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 33957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33957
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_960_length_33957_cov_9.775942"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PK399_01580"
     CDS             complement(<1..787)
                     /locus_tag="PK399_01580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082362.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPW11850.1"
                     /translation="MATTTLNPSEISELIKTRIEKVKLSAEARNEGTVTSVSDGIVRV
                     HGLADVMQGEMIELPNSTFALALNLERDSVGAVVLGDYKHLREGDTAKTTGQILSVPT
                     GPELLGRVVNALGEAIDGKGPIEAKVSAPVETIAPGVIWRKSVSQPVQTGYKSVDSMI
                     PIGRGQRELVIGDRQTGKTALAIDAIINQKHSGIKCIYVAIGQKNSTIANIVRKLEQN
                     GAMAYTTVVAASASESAAMQYIAPYAGCTMGEYFRDRGEDALII"
     gene            complement(852..1379)
                     /locus_tag="PK399_01585"
     CDS             complement(852..1379)
                     /locus_tag="PK399_01585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HPW11851.1"
                     /translation="MSQALTLARPYARAAFAIARDTNALPAWSDALAFAARVAADPAV
                     AALLGNPGLTQADATTLLAPDGADALFGNFLGLLFENRRLSLLPEITGQFDELRFEAE
                     RVVKAKVTSAVALPAAELATIKAALAKRFGREVEVETAVDESLIGGAVIDAGDVVIDG
                     SLKGKLGRLQAALAH"
     gene            complement(1390..1863)
                     /locus_tag="PK399_01590"
     CDS             complement(1390..1863)
                     /locus_tag="PK399_01590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPW11852.1"
                     /translation="MNPTFMTLLGQMITFAILIWFTVKFIWPPLMSAIEERQQKIAEG
                     LAAADNAQKNLAQAQDKVNEELKAARTKANEIIDQAHQRANQLIDAAKADAIAEGNRQ
                     KALAEAEIVSAANRAKEDLRKQVSVLAVSGAEKLLKREIDANAHKALIDDLAAQL"
     gene            complement(1992..2270)
                     /gene="atpE"
                     /locus_tag="PK399_01595"
     CDS             complement(1992..2270)
                     /gene="atpE"
                     /locus_tag="PK399_01595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HPW11853.1"
                     /translation="MEALANVQAFTAVAIGIIVGLGALGAALGIGIMGSKFLESAARQ
                     PELVPMLQGRMFLLAGLIDAAFLIGVGVAMMFAFANPLLAAVQAATGA"
     gene            complement(2361..3203)
                     /gene="atpB"
                     /locus_tag="PK399_01600"
     CDS             complement(2361..3203)
                     /gene="atpB"
                     /locus_tag="PK399_01600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPW11854.1"
                     /translation="MALEPSGGESGGGGLTAYIVHHLTHNVVYVAGQKFHFDSWMVAL
                     LLGLLGSFLLWTQARKATAGVPGKAQAFVEIVVEFVDSQVKDTFHGDRRFIAPLALTI
                     FVWVFFMNAMDLLPLDLPAGVVTAVAGEEAAHHTYLRWVPTADVNTTFAMSITVFFLI
                     IFYSIKAKGGWGFTKELFTAPFHAHGTFAKIVLAGPNLFLNLVEYLSKPVSLGMRLFG
                     NMYAGELVFMLIAGLFMSWITFPFGLVANSVWAIFHILIILLQAFIFMVLTVVYLAMA
                     HEHH"
     gene            complement(4024..5871)
                     /gene="lpdA"
                     /locus_tag="PK399_01605"
     CDS             complement(4024..5871)
                     /gene="lpdA"
                     /locus_tag="PK399_01605"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPW11855.1"
                     /translation="MADIESKVPDIGSHGDVPVIEILVAVGDTVKKDQGLVTLESDKA
                     TMEVPAIADGVIKALKVKLGDAVSEGAVIAVIAASDSGAAVQSAAGSSPPPAQAGGGR
                     EGVAPAAPSEKSTPPQPSPASAGEGAKPAPQAASSSGRTADITCATVVLGAGPGGYTA
                     AFRSADLGVDTVLIERYASLGGVCLNVGCIPSKALLHAAEVIDQAAHAKDYGVDFGTP
                     TINIDALRSYKEKVVGQLTKGLSGMAKQRKVRVVQGTAKFLSANELEIVGEDGKAQVL
                     RFANCIIAAGSQPVKLPSFPWDDPRVMDSTDALNLADVPKTLLVVGGGIIGLEMATVY
                     RALGSDVTVVEFMPQLMPGADLDLVKPLADRLKKQGVSVHLKTKVVEARAGKKGIEVA
                     FEGESIPAGSLYDRVLVSVGRAPNGGKLDAGKAGVAVTDRGFIEVDRQMRTNVPHIFA
                     IGDLVGQPMLAHKATHEGKLAAEVAAGEKKEWVARVIPSVAYTDPEIAWVGVTETEAK
                     AKGLKVGVGKFPWAASGRAIGLSRTEGFTKLVFDEASHRIIGAGIVGVHAGELIAELG
                     LAIEMGCEVEDIGHTIHPHPTLSESVGMAAEVYDGTITDLYIPKAKSRA"
     gene            complement(5882..7279)
                     /locus_tag="PK399_01610"
     CDS             complement(5882..7279)
                     /locus_tag="PK399_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HPW11856.1"
                     /translation="MADLKEARVPDIGGHDDVPIIEVLVKVGDTVAVDQGLVTLESDK
                     ATMEVPSPFAGVVRELKVKLGDNLSEGALVAIIEVAAGVAAEAPAPASVLPPPPAAQP
                     IAAVATTSASETGAKVEPVVVAPPAPVPAPAAAPMGAGLPPVRFEADAVLPDKVPYAS
                     PAVRLFARELGVDLMQVTGSERGGRIGKLDVQNFVKSALAGGVSAAPAAVSAGGGGGL
                     NLIPWPKVDFAKFGAIESKPLSRIQKISGANLARNWAMIPHVTQFDDADITDLEDLRV
                     ALNNENAKAIAAGKAGKVTMLAFMIKASVAALKKYPNFNASLDGDNLVLKQYYNIGFA
                     ADTPNGLVVPVLRDADKKGVMQIAAEMGELAAAARDGKLKPEQMQGGCFTISSLGGIG
                     GTAFTPIINAPEVAILGVSKSETKPVWDGKAFQPRLIVPLSLSYDHRVIDGAAAARFT
                     AYLAQLLADMRRAML"
     gene            complement(7450..8301)
                     /locus_tag="PK399_01615"
     CDS             complement(7450..8301)
                     /locus_tag="PK399_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011348672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW11857.1"
                     /translation="MLLMLVSILLFSLMDAGLKSLSAHYPPLQVATLRAMSSLPLVLA
                     WALSTAGWRALVRVRWPLHLLRGVLGIMMMGSFVYALKRLPLSTAYSIFFVAPLLITA
                     LSVPILGERVGPRRWTAIAIGLLGVLVVLRPTGEGVLTTAGFAVLVAALGYAVSAITV
                     RVLARTDSTQAITFWLLALMGLGAGALAWPDWVPLRAEHAWLIGGIGIAGAIGQYAIT
                     EAFRLGEASLIAPLEYTALVWGVALDLALWGVLPDAITWLGAAIIIASGLYLIRREKV
                     HAEAEHP"
     gene            8397..8879
                     /locus_tag="PK399_01620"
     CDS             8397..8879
                     /locus_tag="PK399_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HPW11858.1"
                     /translation="MPSFDIVSEVDTHELTNAVDQANRELSTRFDFKGVVASFELDDA
                     AIKQSAPSEFQLQQMTDILRARLIARKIDARCLEFGDIETNLAGARQTVTVKQGIERE
                     LAKKLQAAMKDAKLKVDSQINGDKLRVTGKKRDDLQAAMALLRAGEFGVPLQFDNFRD
                     "
     gene            8934..9485
                     /locus_tag="PK399_01625"
     CDS             8934..9485
                     /locus_tag="PK399_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HPW11859.1"
                     /translation="MTDTFDPAQIETDVRRWLERAVIGLNLCPFAKAVYVKDQVRIVI
                     SDASTERALLEELGEELALLRDTPAEQVDTTLLVHPQVLGDFLDYNDFLGDADALVEA
                     MDLDGVLQVASFHPQYQFAGSQPDDADNLTNRAPYPILHLLREASIDRAVAAYPDPDA
                     IIARNIATVRELGVEGWSKLLAE"
     gene            complement(9490..10179)
                     /locus_tag="PK399_01630"
     CDS             complement(9490..10179)
                     /locus_tag="PK399_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017172894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPW11860.1"
                     /translation="MTPVAIPPLPFLPEAVVFDMDGLMLDSERAINDCMARAASDLGH
                     DLPASLWLQMIGGGDGLCRRLLAERIGADATMELLARAEAMYDVVADAGIDHRPGIVE
                     LLDWLVARGLPRAVATSTRRPLAMRKLAAAGLLPYFHAVATSSDVAAPKPAPDVYLLA
                     ASKLGIAPARCLVLEDSPTGVRAALAAGMTPVQVPDLLEPDDAARALGHRIVASLGDA
                     QRLLEARLSEA"
     gene            complement(10176..10886)
                     /locus_tag="PK399_01635"
     CDS             complement(10176..10886)
                     /locus_tag="PK399_01635"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW11861.1"
                     /translation="MKLVKHIANLGYGSRKDVQWMFREGRITDADGEVLYADDPLDHD
                     NVRVDGEPLDPPPGLLLMLNKPVGYTCSMKDRGRVVFDLLPARFRERNPALSTVGRLD
                     RDTSGLLLFTDDGQLLHRIISPKSKLPKVYVATLAEDLRGDERATFASGSLMLESETT
                     PLLPAELEPLEARRARLVLHEGRYHQVRRMFAAIGHHVQALHRERIGGLTLGDLPEGQ
                     WRALGAADRDRLFATEPA"
     gene            complement(11004..12068)
                     /locus_tag="PK399_01640"
     CDS             complement(11004..12068)
                     /locus_tag="PK399_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPW11862.1"
                     /translation="MASMHRDIALDALLHPFAEGALRWPPAGDALFLRAREGAALHAR
                     GGLQALACTQPFKPEADRLQRIGVGLIDEDALPAARYPLVLVLPPRQREEARALLAKA
                     CSAVAPGGSVVASVANDEGAKSREADLKQLAGNLSVISKHHCRVFWTRPDAMFDPATL
                     AQWAKADAPRRVISPNLPGGSFLSRPGVFAWDRVDAASAMLAQALPNDLGGRVADLGA
                     GWGYLSMQVLARCPQVASLDLYEADARALALAEANLADARVAVQRHWHDVAAGVSERF
                     DAIVCNPPFHALGRGDRPDIGRAFIAAAATALKPGGRLWLVANRHLPYEHALGEGFAQ
                     VQTLAQDGGFKIVEGEKARA"
     gene            complement(12099..12524)
                     /locus_tag="PK399_01645"
     CDS             complement(12099..12524)
                     /locus_tag="PK399_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11863.1"
                     /translation="MNIHDREIPLDEQRWQAQERARRGDPDADATDLRIARALRHAPS
                     VDLPVDFASRVAALARTQATFSAVLEQRLLRGLVLVFALSAAVVVAWFGRGWVAELAT
                     VLPGGQDAVAWSGIATLCVLGNWGVGLLRGRLASEASAA"
     gene            complement(12521..13102)
                     /locus_tag="PK399_01650"
     CDS             complement(12521..13102)
                     /locus_tag="PK399_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW11864.1"
                     /translation="MDADRALVEAVLANRAGAFEQLVREYQGLCWHVVQRMVRHPEDT
                     RELCQEAFLRVHRYLHQYRFDSPLKSWIGQVAYSVAKRHLERKRIALADVHVGAGMDE
                     DDGPSLLDNLGDGTDLQEAHAQAGEERELHAAIERLPPLQRTILTLYHLEELPIGEIA
                     TITGLAEGTIKSHLFRSRNKLREILAPRIGVAA"
     gene            13247..13573
                     /locus_tag="PK399_01655"
     CDS             13247..13573
                     /locus_tag="PK399_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11865.1"
                     /translation="MNFELLIPITLFACIAYSIKAVVDARVRKQLVSSNGAPDLVRSI
                     LEGDEVNRRLSSLRWGITLVALSIGFAIVEAAGWREITPGVIAVLVGAVGIGNLGSFA
                     AARKLG"
     gene            13624..13911
                     /locus_tag="PK399_01660"
     CDS             13624..13911
                     /locus_tag="PK399_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HPW11866.1"
                     /translation="MTDASNATTDTPPDHLSNDPRSKFHDAAVLERGIGIRFNGMERN
                     NVEEYCISEGWVRMAAGRSLDRRGQPMTMKFKGTVEAWFLDSVASDETPAD"
     gene            13983..14543
                     /locus_tag="PK399_01665"
     CDS             13983..14543
                     /locus_tag="PK399_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3016 domain-containing protein"
                     /protein_id="HPW11867.1"
                     /translation="MNRLRTLPLLLATVLALGACASTGNTSMLAADAPRALPAAGPVS
                     VSWEDPARFTEVRHSGNRWAATEGNWLNTLATYMRKEAEETLPAGHRLELTIVDIKRA
                     GQYEPWLGPNLQDTRIIRDMYPPRMTLRFREVDADGTVLAEGERKLSDPAFLMNASRV
                     NDTDPLRYEKRMVDSWLRSDWRTAAR"
     gene            complement(14593..15861)
                     /locus_tag="PK399_01670"
     CDS             complement(14593..15861)
                     /locus_tag="PK399_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPW11868.1"
                     /translation="MNAAAVHRRLDALWRMESPKLLARLARITGNIDTAEELAQDVLV
                     VALERWPREGVPDNPAAWLMTAAKHRAIDHVRQRQLHARKQDEYGVELELHGADMQGD
                     DAQRLADDDIGDDLLRLLFTSCHPLLPMESRVALTLRMLGGLSTPEIARAFLQPEPTV
                     AQRIVRAKRTLAEKQVPFEVPRKAELPERLDAVLEVLYLVFNEGYAATSGDDWMRPAL
                     CEEALRLGRVLVGLLPNETEALGLLALMELQASRMPARTQADGTPILLPQQNRSRWDR
                     LQIQRGLQALVRAQRLGGADGPYALQAALAACHARARRFDDTDWTAIAALYARLMQVA
                     PSPVVELNRAVAVAKAQGPQAALAIVEGLAAEPKLRDYAPLPAVRGDLLQQLGRLDEA
                     RVEFERAAALTANARESTVLLARAAACDAA"
     gene            complement(15905..16291)
                     /locus_tag="PK399_01675"
     CDS             complement(15905..16291)
                     /locus_tag="PK399_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW11869.1"
                     /translation="MNASNPVVWFEIYTDDLARARAFYEAVLGVTLEPLPMPGGMDGF
                     QMLAFPANMESHGASGTLCKMEGVSPGVGGTLVYFSCADCAVEAGRVEAAGGRLHQAK
                     MSIGEYGSIALAFDTEGNMFGLHSMG"
     gene            complement(16352..16759)
                     /locus_tag="PK399_01680"
     CDS             complement(16352..16759)
                     /locus_tag="PK399_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HPW11870.1"
                     /translation="MPRNLYVNLPVKDLDRTVEFFTVLGFAFNPMFTDENAACMIVND
                     NTSVMLLVESFFATFTRKPIADAKAVTETLLALSLDSRAEVDDFVARAIAKGAVEYAE
                     PRDLGFMYQRGIADLDGHQWEVFFMDESQFPGA"
     gene            complement(16786..17193)
                     /locus_tag="PK399_01685"
     CDS             complement(16786..17193)
                     /locus_tag="PK399_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW11871.1"
                     /translation="MKLIPYLMFREGKCQEAFAFYARALGGEIVSTATYGDMGPDIPE
                     PMRPFIANIQLLAGGAMIMGADAMENPEGQATTSVNIEVDSIEDAERIFAAMSDGGQV
                     RMPLAESQWALRFGMFDDRYGQPWMINLMKPAP"
     gene            complement(17219..17638)
                     /locus_tag="PK399_01690"
     CDS             complement(17219..17638)
                     /locus_tag="PK399_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPW11872.1"
                     /translation="MKVMVLVKATDASEAGVMPTAELFEAMGKFNEELVNAGVMLAGE
                     GLKPTSHGTRIAFDGPSRRVIDGPFAETRELVAGFWLWQVRSMDEAIEWAKRCPNPMP
                     GPSELEIRPLYEAEDFGTEFTPELREQEDRLRERLGN"
     gene            17842..18219
                     /locus_tag="PK399_01695"
     CDS             17842..18219
                     /locus_tag="PK399_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPW11873.1"
                     /translation="MQFLTLIYIDPERLAELPAEEYDRLMRGCFEKADALRDAGCLHG
                     SQQLQDPGSARTLRVRDGVSRITDGPFAETREFLAGFNLVEAADIEAAMEIARAFPWS
                     QFGSIEVRPVRDMQAVRVQVGAH"
     gene            18319..19167
                     /locus_tag="PK399_01700"
     CDS             18319..19167
                     /locus_tag="PK399_01700"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HPW11874.1"
                     /translation="MDLRPALLALLLLASQPAAHAASPLRAAPAPMLASRITDDIDIA
                     AWLVSEKLDGVRARWDGRQLVTRAGNRIDAPAWFTAGWPAQAIDGELWIGRGRFQDIS
                     DLVRALRGDAAGWRQVRFMAFDLPASPHAFASRTRELRVLVEETASPRLQRIAQLHFS
                     ERAQLDAWLRSVASGGGEGLMLHHANAHYRAGRSDQLLKYKRWDDAEARVVGYRPGKG
                     KYHGMVGALLVEDAHGRRFALGSGLHDAERAAPPAIGSTVTFRYDGLTAKGTPRFARY
                     LRVRRE"
     gene            complement(19190..19954)
                     /locus_tag="PK399_01705"
     CDS             complement(19190..19954)
                     /locus_tag="PK399_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11875.1"
                     /translation="MRTLLLASCVAVALLSGCAGVSINESAGIERVLANPARSASDRE
                     RDARDKPAEVLALARFKRGDTVADILSGGGYYAEILSGIVGPGGKVLLVNNPGYENFG
                     KKGLAERLAANRLPNLTHVVGPSDALGMGDRVLDGAVIVMSYHDLYWVDEKEGWPRVD
                     AGLFLDQVARALKPGGVLLVVDHSARQGTGSADAQALHRIDEQFAIADFRKHGLEWEA
                     AIPVLRNPDDDRSKNVFDPAIRGRTDRFVHLYRKPE"
     gene            complement(20048..21157)
                     /locus_tag="PK399_01710"
     CDS             complement(20048..21157)
                     /locus_tag="PK399_01710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPW11876.1"
                     /translation="MSQPAIDNLRTDAPGLPWAEQEARDRRARLTELLARVSREAAEG
                     EDLEAVLRRIVDCLVERMPVALASIVLLNEACTHFVHEVWAGELTLNVLQPADDWPVS
                     KGAAGRCARTGVAQLIANVDLDPDYLAGNSEVRSEYLVPIRHRGRLHGVLNIESTQSD
                     FFSAEACEVFDAIAAQVAGTIHLARVVRELEVANRKLQALSMQDGLTGIANRRQFDTA
                     LQQVWARLVKGSVPLALLMADADWFKALNDTAGHLHGDECLRALARLCAAQLRDEDDL
                     VARFGGEEFVLLLPGRDLEAAVAVGEALRKAVARERLAHPASPIAPYVTVSIGAAATW
                     PSEGGEPDGLLLAADRAMYAAKARGRNCVVAKYVG"
     gene            21372..22619
                     /locus_tag="PK399_01715"
     CDS             21372..22619
                     /locus_tag="PK399_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPW11877.1"
                     /translation="MFKSIGFVLRIAIAWGLVVLVVASLYSSIPLLGRFELPIVLVSM
                     ATMALVVGGAFSHLRRVRLIAGHIDGDALGNRQKRLVEIPLEAGEAFDLLDAAIRELP
                     GAEQIQSARDSLQVKAKVARPRTYGEHPLRRWNPLLWFGLPRNQLQATVTPAGDSGRV
                     TLICEPENPAWSDWFLVDDGTNYENAEAIVRAITRRIGERRRGEQASAAQTATEKELT
                     EAKLNLLHAQVEPHFLYNTLASAQLLTRSDPVRAEAMLGHLIQYLRRSLPNAEGEMST
                     LGAELERALAYLEILKVRMGDRLDVQTDVAEALRNTPMPAMMLQTLVENAIKHGLEPR
                     TGGGTVWIRARRDDNTVAVTVADNGEGFNSKTSGTGIGLKNVRERLRLRYAGEANLAV
                     VANFPAGVAATLTVPANGRNAHG"
     gene            22612..23385
                     /locus_tag="PK399_01720"
     CDS             22612..23385
                     /locus_tag="PK399_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPW11878.1"
                     /translation="MADIRTCIVAEDEALLRHALVAELQRAWPSLRIVAECEDGASAV
                     EALAEHQPDVAFLDIRMPGLTGLEVATTAAEASPRTRIVFVTAYDQYAIDAFDRGAID
                     YLLKPVKPERLDATVARLQAQDTLPDAATLAALLDKLGAMPDPAGSPEPLTWLTASAG
                     RETRLVLVDEVIYFHADQKYTTVVTADGEALLRTPLRELLPRLDPATFKQIHRSTIVN
                     LKAVAGILRDDSGRGTLRLKHRPETLAVSQPFMALFKQM"
     gene            23449..23814
                     /locus_tag="PK399_01725"
     CDS             23449..23814
                     /locus_tag="PK399_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11879.1"
                     /translation="MSKFIALLQFLLALSGCSIGGTTYTSRIDSDQVHLTSKAHVQDG
                     VARFDCVESSSGICHYTLYPEACSGKADCQLAPLQRFSVPSGESHQAAGLIDFRPCVA
                     TTAAALGADCQPATAGKAR"
     gene            23814..24794
                     /locus_tag="PK399_01730"
     CDS             23814..24794
                     /locus_tag="PK399_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11880.1"
                     /translation="MALRRFLRLAPKVMLGLVLLAIVGVAVLLGPYAWRVEHIAADAR
                     VGFRSDYYLYVPETLHRDANGKAVLLVQPNNSGRTSDDIGLHRRDAWMTGFERKRIAD
                     ELGVALLVPAFPRTASDWKVYTHALDRDVLTTREPLLERLDLQLIAMIDDARVRLRNA
                     GTPVDEKVLLQGFSASAMFANRFTALHPDRVRAASIGSPGGWPIAPVAQVGVDALPYP
                     AGVADLSALTGRPFDAAAFARVPQYLYMGDADDNDSLDFEDGWDTPMSAEVDRLFGDT
                     PLARWTHAEALYARAGADARFELVPGIGHDRKALQERSTEFFQRVLTTPR"
     gene            24829..25416
                     /locus_tag="PK399_01735"
     CDS             24829..25416
                     /locus_tag="PK399_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HPW11881.1"
                     /translation="MNYLAHAWLARHSDDAILGAILGDFVFGQSALQDWPPAVRAEIV
                     RHRRIDQYTDAHPAVVAARGLFDAAGLRRYAGIVLDVYFDHCLARGWVRWNDAPLGDF
                     TARVYRVLDAHREELPPRLQAIAPRMAAHDWLGSYAQRGNVDHAVRGIATRLSRNGDK
                     LVACLDVLRVEETAIDAAFGAFFPDLVHAAARMRP"
     gene            25451..27427
                     /locus_tag="PK399_01740"
     CDS             25451..27427
                     /locus_tag="PK399_01740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005916330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPW11882.1"
                     /translation="MRTRILGLLALVLLIPAAHAQVKLDPYLKQDGYGRIKISPDGQY
                     YAATVQLEDSVGLALLRRSDKKIVNGASGIKDSVVADFWWVNDHQVVIAMAERFGSRD
                     QPYRTGQLFSIGVDGKRGKQLVGPKPQPGLVTVIGNAGPWEMTDLIDSLPGDDENVLI
                     SAWKLGTNPLTTVDLMDVNNGRRKRIATAPVNRAVFLTDAQGQVRFAQGAQNDNYSKL
                     YYRDDNQSEWRLVNDESSSRRVDTPVGFSADGRIAYFNSSTEDGPGALVAWDIATDDR
                     KQVLRDATVDPYAIIHDRDGHTVLGVQFMSGGVQTRMLDDSAPASRVYRALAKAFPDH
                     AVNVTSHTRDGVALVQAWNDRTPGDTYLFDPAKMSAKDVFIQREWFDPAKLPKARAIT
                     VKARDGLVLHGYLTLPFGAKETTPLPMVVLPHGGPFGIFDQWAFDDDTQLLAEAGYAV
                     LRINFRGSGNYGRAFHQAGAREWGGAMQDDLTDATRWAIAERIADPARICIYGASYGG
                     YAALMGVAKEPDLYRCAVGYVGVYDLEAKHREDSRDAKWVRNWMDDWVGERSTLAARS
                     PTEMASRIKVPVLLAAGGADVIAPISHSKAMARALRDAGKPVQTLYFDSEGHGFYTED
                     HRRAFYTTLLDFLADNIGGTRAAKAAGESSPGGT"
     gene            complement(27580..27774)
                     /locus_tag="PK399_01745"
     CDS             complement(27580..27774)
                     /locus_tag="PK399_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11883.1"
                     /translation="MFRTQSPTPAAYVERRLTPRRGDGGAEPARIHRERDFGVGYGNS
                     SGYASNRSYTTPQATYFRCR"
     gene            27914..28528
                     /locus_tag="PK399_01750"
     CDS             27914..28528
                     /locus_tag="PK399_01750"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPW11884.1"
                     /translation="MPKAAAALQRCFWSDGSDLYDGYHDHEWGRPVTDDVRLFEKISL
                     EGFQAGLSWITILKKRDNFRAGFDGFDFHKVARYTERDVERLLGDAGIVRHRGKIEAA
                     IHNAGRAIELQREFGSLARYFWQYEVDTRKRPKKLTVDVLRTFTTAPEAIALSKDLKK
                     RGWKFVGPTTMYAFMQAMGLVNDHQHDCHVRGEVEAKRKALQRP"
     gene            28600..29376
                     /locus_tag="PK399_01755"
     CDS             28600..29376
                     /locus_tag="PK399_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11885.1"
                     /translation="MRLLVLLAAIAMPVVAWLTNSGTFGPDNGTMSDRYPTLLVAAGY
                     AFAIWGLIFLLDLAYAGWQLTGTRKSDDTLTRIAPWAAGGFALTTLWMPLFAMSQFWL
                     CLLVIFGATFCLIRCAMILSHDRTPQEGQWMWAWLPLSLHAGWLSLAAFLNLAQVVVA
                     YELASTTQQLPWSLAIFGLAALMLLMVNLRMRGNVDYVVAALWALAAVYVKQSGNDMP
                     GAQTAAWVAVGIAVVLVLQTVMLRRKYPGGLLPNPARAPG"
     gene            29467..30066
                     /gene="lexA"
                     /locus_tag="PK399_01760"
     CDS             29467..30066
                     /gene="lexA"
                     /locus_tag="PK399_01760"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007971824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPW11886.1"
                     /translation="MDHLSPQRAAVLAFLQRELVAGRSPSLAEIALAFGFASRNAAQK
                     HVQALVADGLLEQATGRSRGLRLPGGPADLLPLAVLGRVAAGQPLGADIGLDQQLWLD
                     RRLFSPTPDYLLKVQGDSMIDDGILEGDLVGVQRSADARDGQTVIARIDGELTIKRLQ
                     RSARRLRLLPRNPAHAPIEIERGQDFAIEGVYCGLLRQG"
     gene            30068..30679
                     /gene="imuA"
                     /locus_tag="PK399_01765"
     CDS             30068..30679
                     /gene="imuA"
                     /locus_tag="PK399_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HPW11887.1"
                     /translation="MQAVASLDSLLAAQTLWHAGRHPATTRDGEPTGHAALDALLPQR
                     GWPRRALTELLLPADGVGELALLMPTLARLSREGGTLALVAPPYLPYAPAWQAAGIDL
                     GALQVIETTPRDALWAFEQCLRSGACAAVVGWPRQADAQALRRLQVAADSGDCLGFVL
                     RDRKHAANPSPAALRLEHDSDGWRVRKCRGGPAPTRAFAPGLH"
     gene            30689..>31699
                     /locus_tag="PK399_01770"
     CDS             30689..>31699
                     /locus_tag="PK399_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HPW11888.1"
                     /translation="MLWACIVLPQLALDAVLRRLPDPGAPLALASGPTQLRTLHAVNA
                     GAAQAGLRAGMRLAAAHALLPTLATVEHDPQAEARWQQFLAAWAYRHSSLVSAQWASC
                     IVLEVRASFRLLGPWPRIEARLREELAALGFAHRIAMAPTPRAARVLAGLQDGLAVSN
                     PAAMQALLDRVPVRRAVLPGDTGERLQRMGIRDLRTLRELPRESLRRRFGAELLPHLD
                     RLYGDADDALSYYAPPDHFDARIELGHEVESHTALLFPLRRLIGDLCTCLSIRDGGVQ
                     RFVLRLEHDVHRHDTRAHTDVEVGLLAAEREPAMLFEVTRNRLERVSIPKPVVALRLL
                     ARE"
     assembly_gap    31700..31799
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            31921..>33957
                     /locus_tag="PK399_01775"
     CDS             31921..>33957
                     /locus_tag="PK399_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012445640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPW11889.1"
                     /translation="MSWDDAVDGVDRETPGGRMPRAWQVAQRRALPAANDDAPGHDDL
                     PAYAELHCLSDFSFLRGAASAEELFERAHRCGYEALAITDECSLAGIVRGLEASRATG
                     LKLIVGSEFVLSCGMKFVLLVEHAAGYTQLCRLITHARRAAPKGGYLLTRGDVETVLA
                     DADVGLFALWCPAAQPDANEGRWLHAVFGERAHLAVELHREQDDEARLHALLALAHDL
                     QLIPLAAGDVHMDVRRQRVLQDTMTAIRHGMTLADCGEHLFRNGERHLRTRRALGNIH
                     PHHLLDNAVQLARRCRFDLGNDLEYHYPAELVPPGHTPTSWLRARAEQGMRERWPEGV
                     PAKIVAQIDDELALIEYLKYEAFFLTVDDIVRFAKSREILCQGRGSAANSAVCFALGI
                     TVVNPAESRLLMARFLSKERNEPPDIDVDFEHERREEVLQYVYAKYGRERAALAATVI
                     RYRGKSAVRDVAKVFGLPPDQLALLAECYGWGNGDAPMQQRLFEAGFDPDNPLIARVL
                     AVTEQLQGHPRHLSQHVGGFVISDAPLHTVVPVENAAMADRTIIQWDKDDLETMKLLK
                     VDCLALGMLTCIRKAVALIERHRGYTIDLARLPPGDQPTYDMIQAADTVGVFQIESRA
                     QMSMLPRLKPTCFHDLVVEVAIVRPGPIQGGMVHPYLRRRQGLDPVVYPSPGIED"
BASE COUNT         5392 a        11841 c        11333 g         5291 t
ORIGIN      
        1 agatgatcag cgcgtcttcg ccgcggtcgc ggaagtactc gcccatggtg cagccggcgt
       61 acggcgcgat gtactgcatc gccgccgatt cggaggccga cgcggcgacc accgtggtgt
      121 acgccatcgc gccgttctgc tcgagcttgc gcacgatgtt ggcgatggtc gagttcttct
      181 gcccgatcgc cacgtagatg cacttaatgc cgctgtgctt ctggttgatg atcgcgtcga
      241 tcgccagcgc ggtcttgccg gtctggcggt cgccgatgac cagctcgcgc tggccgcggc
      301 cgatcgggat catgctgtcg accgacttgt agccggtctg caccggctgg ctgaccgact
      361 tgcgccagat cacgcccggg gcgatggttt ccaccggcgc gctgaccttg gcctcgatcg
      421 ggcccttgcc gtcgatcgcc tcgcccagcg cgttgaccac gcggccgagc agttccggac
      481 cggtcggcac cgagaggatc tggccggtgg tcttggcggt gtcgccttcg cgcaggtgct
      541 tgtagtcgcc cagtaccacc gcgcccaccg agtcgcgctc caggttcagc gccagcgcga
      601 aggtgctgtt cggcagctcg atcatttcgc cctgcatcac gtcggccagg ccgtggacgc
      661 gcacgatgcc gtcggatacc gaggtcacgg tgccttcgtt gcgcgcttcc gcggaaagct
      721 tgaccttctc gatgcgggtc ttgatcagtt cgctgatttc ggacgggttg agcgtggtgg
      781 tggccatctt ggtttccttg gtgcccgcgt tgccgccggc aagattcaaa tgagtgttcg
      841 caacaatcgg atcagtgcgc cagcgcggcc tgcaggcggc ccagcttgcc cttcagcgaa
      901 ccgtcgatga cgacgtcgcc ggcatcgatc accgcgccgc cgatcaggct ctcatcgacc
      961 gccgtctcga cctcgacctc gcggccgaag cgcttcgcca gcgcggcctt gatggtggcg
     1021 agctcggctg ccggcagtgc gaccgcggag gtcaccttgg ccttgaccac gcgttcggcc
     1081 tcgaagcgca gctcgtcgaa ctggccagtg atttccggca gcagcgacag gcgacggttc
     1141 tcgaacagca ggccgaggaa gttgccgaac aacgcgtccg cgccgtcagg cgcgagcagc
     1201 gtggtggcat ctgcctgggt cagccccggg ttgccgagca acgcagcgac cgcgggatcc
     1261 gcggccacgc gggcggcgaa cgccagcgcg tccgaccatg cgggcagcgc gttcgtgtcg
     1321 cgcgcgatcg cgaacgcggc gcgggcgtag ggacgggcga gggtcagggc ctggctcatc
     1381 ggtcgtgcct cagagctgcg ccgccagatc gtcgatcagc gccttgtggg cgttggcgtc
     1441 gatctcgcgc ttcagcagct tctcggcgcc ggacacggcc agcacggaca cctgcttgcg
     1501 caggtcttcc ttggcgcggt tggcggcgga cacgatctcc gcttcggcca acgccttctg
     1561 gcggttgccc tcggcgatcg cgtcggcctt ggccgcgtcg atcagctggt tggcgcgctg
     1621 gtgggcctgg tcgatgatct cgttggcctt ggtgcgcgcg gccttgagct cctcgttgac
     1681 cttgtcctgc gcctgcgcca ggttcttctg cgcgttgtcg gcggcagcga ggccttcggc
     1741 gatcttctgc tggcgttcct cgatcgccga catcagcggc ggccagatga acttgacggt
     1801 gaaccagatg aggatcgcga acgtgatcat ctggcccagc aatgtcatga aggtcggatt
     1861 catgtcgtac cttgcgtgtc gaacgggcgg cgcgatgcgc gcccgtcagg ggcaacgcca
     1921 cggcggcatg ccgcggcgct tgaatcgccg gtgcgcgcga cgacggccgc gcgcaccgtg
     1981 acgcgtaccg cttaggcgcc ggtggcggcc tgcacggcgg ccagcagcgg gttcgcgaac
     2041 gcgaacatca tcgccacgcc gacgccgatc aggaacgccg cgtcgatcag gccggcgagc
     2101 aggaacatgc ggccctgcag catcgggacc agttccggct ggcgggcagc ggattcgagg
     2161 aacttcgagc ccatgatgcc gatgccgagg gcggcgccga gcgcacccag gccgacgatg
     2221 atgccgatgg cgacggcggt gaaggcctgg acgttggcaa gtgcttccat ggtggatctc
     2281 cggttttcag tgctgagcag tggaaagtga aaggggttgg aacgagttga agcggaacgc
     2341 gtgggtctgc agcgggcgga tcagtggtgc tcgtgggcca tcgccaggta gacgacggtc
     2401 agcaccatga agatgaaggc ctgcagcagg atgatcagga tgtggaagat cgcccacacc
     2461 gagttggcca ccaggccgaa cgggaaggtg atccacgaca tgaagaggcc ggcgatgagc
     2521 atgaagacca gctcgcccgc gtacatgttg ccgaacagtc gcatgcccag gctgaccggc
     2581 ttggacaggt actcgaccag gttgaggaac aggttcgggc ccgccagcac gatcttggcg
     2641 aaggtgccgt gcgcgtggaa cggcgcggtg aacagctcct tggtgaagcc ccagccgccc
     2701 ttggccttga tcgaatagaa gatgatcagg aagaacacgg tgatcgacat cgcgaacgtg
     2761 gtgttcacgt cggcggtcgg cacccagcgc aggtaggtgt ggtgcgcggc ttcctcgccg
     2821 gcgaccgcgg tgaccacgcc cgcgggcagg tcgagcggca ggaggtccat ggcgttcatg
     2881 aagaacaccc agacgaagat ggtcagcgcc agcggcgcga tgaagcgacg gtcgccgtgg
     2941 aaggtgtcct tgacctgcga gtcgacgaac tcgaccacga tctcgacgaa ggcctgcgcc
     3001 ttgcccggca cgccggcggt cgccttgcgg gcctgcgtcc acagcaggaa gctgcccagc
     3061 aggcccagca gcagcgcgac catccacgag tcgaagtgga atttctgccc ggccacgtag
     3121 accacgttgt gggtcaggtg gtggacgatg taagcggtca ggccgccacc gccggattcg
     3181 ccgccgctcg gttccagggc catgctgttc aggatcgcgc cttccaggat cacgtactcg
     3241 ccctcgtcgt gctcggcctt gccgccgcgt acttcatcga aaccacgaat gccgccgcgg
     3301 ccatcgccgc accggccagc gccggcactg ccggcaaccg ccaaaccgcc atcgccaggt
     3361 acaagcccgc ggcgaccacc aaccacttca gcgcggtgcc gagcagcaag cgccccagcg
     3421 ccgtgcctgc cccggccacc ccacccccca acccccccac ccccgaacgc cccccaggcc
     3481 gccagcaggc tgcccagcgc catcgacccg cccccgacca gcgccgccaa ggcggcatcc
     3541 ggcccgagga ggaagcaggc tgcggcagtc agcagcgcgg cgacagcctg gaaggccacc
     3601 acccgcagcg cctgccgcca gccctcggca gcggaattca gcacgcggaa acttcccgag
     3661 gaacaaccag acatcggtgc ggtgccgcag tcaggttggc cgaaacgcac caaaaccgtc
     3721 ctgcggagcc gcaaaagtat agcaggcgtg aaaaaccggg ggcaaccgcc aaaggccctt
     3781 gcaaacaagc ggaacctttc acgtggggcc ttggtcagag cccgtgcgcc gcccctcctc
     3841 gtcagctccg ggcgtgcgtc tggagccccg gcccgcgccg gggcttcttt tcttggccgg
     3901 aattcgaagc cgagttagcc aggcgtctag gctcgcgcat ccatgcgctc gccaagtcgc
     3961 acggcctcgg ccatcacggc cgaggcgctc aaagccgcac aacgtgccct tcaaggcacg
     4021 ttgtcaggcg cggcttttcg ccttcgggat atagaggtcg gtgatcgtgc cgtcgtagac
     4081 ctcggcggcc atgcccaccg actcgctcag ggtgggatgc gggtggatgg tgtggccgat
     4141 atcctcgacc tcgcagccca tttcgatcgc caacccgagt tcggcgatca gttcgcccgc
     4201 gtgcacgccg acgatgcccg cgccaatgat gcggtggctg gcctcatcga acaccagctt
     4261 ggtgaagccc tcggtgcgcg acaggccgat ggcgcgcccg gacgccgccc acgggaactt
     4321 gcccaccccg accttcaggc ccttggcctt cgcctcggtc tcggtgacgc cgacccaggc
     4381 gatttccgga tcggtgtagg ccacgctcgg gatcacccgc gccacccact ccttcttttc
     4441 gccggccgcg acttcggccg cgagcttgcc ctcgtgcgtg gccttgtggg cgagcatcgg
     4501 ctgcccgacc aggtcgccga tcgcgaagat gtgcggcacg ttggtgcgca tctgccggtc
     4561 gacctcgatg aagccgcgat cggtcaccgc gaccccggcc ttgcccgcgt cgagcttgcc
     4621 gccgttcggc gcacgcccca ccgacaccag cacgcggtcg tacagcgagc ccgcggggat
     4681 gctctcgccc tcgaacgcga cctcgatgcc cttcttgccc gccctggcct cgaccacctt
     4741 ggtcttgagg tgcacggaaa cgccctgctt cttcaaccga tccgccagcg gcttgaccag
     4801 gtcgaggtcc gcgcccggca tcagctgcgg catgaactcg acgaccgtca catcgctgcc
     4861 cagcgcgcgg tacaccgtgg ccatctccag gccgatgatg ccgccgccga cgaccagcag
     4921 cgtcttcggt acgtcggcca gattgagagc gtctgtggaa tccatcaccc gcgggtcgtc
     4981 ccacgggaac gacggcagct tcaccggctg cgagccggcg gcgatgatgc agttggcgaa
     5041 gcgaagcacc tgcgccttgc cgtcttcgcc gacgatttcg agctcgttcg ccgacaggaa
     5101 tttcgcggtg ccctgcacga cgcgcacctt gcgctgcttg gccatgccgg acaggccctt
     5161 ggtgagctgg ccgacgactt tttccttgta gctgcgcaag gcgtcgatgt tgatcgtcgg
     5221 cgtgccgaaa tcgacgccgt aatccttggc gtgcgcggcc tggtcgatca cctctgccgc
     5281 atgcagcagc gccttggacg ggatgcagcc gacgttgagg cagacgccac caaggctcgc
     5341 gtagcgctcg atcagcacgg tgtcgacgcc gaggtcggcg ctgcggaacg cggcggtgta
     5401 gccgcccgga ccggcaccga gcacgaccgt ggcacatgtg atgtcggccg tacggccgga
     5461 cgaggaagcg gcctgcggcg cgggctttgc tccctcccct gcggatgcag gggagggctg
     5521 gggtggggtg ctcttctcgc ttggtgcagc aggagcaacc ccctcccgac ctccccctgc
     5581 ctgcgcaggg ggaggagagg aacccgcagc gctttggaca gccgctccac tgtcagaagc
     5641 cgcaatcacc gcgatcaccg cgccttccga caccgcatcg cccaacttca ccttcagcgc
     5701 cttgatcacg ccatcggcga tcgccggcac ttccatggtg gccttgtcgg attccagggt
     5761 gaccaggccc tggtccttct tcaccgtgtc gccaaccgcg accaggattt cgatgaccgg
     5821 gacgtcgccg tggctgccga tgtccggaac cttgctttcg atgtctgcca tggccagccc
     5881 cttacagcat ggcccgacgc atgtcggcca gcagttgtgc gagataggcg gtgaagcgcg
     5941 cggcggcggc gccgtcgatc acccggtggt cgtagctcag cgacagcggc acgatcaggc
     6001 gtggctggaa ggccttgccg tcccagaccg gcttggtctc cgacttcgac acgcccagga
     6061 tcgcgacttc cggcgcattg atgatcgggg tgaacgcggt gccgccgatg ccgcccagcg
     6121 aactgatggt gaagcagccg ccctgcatct gctcgggctt gagcttgccg tcgcgcgcgg
     6181 cggcggcgag ttcgcccatc tcggcggcga tctgcatcac gcccttcttg tcggcgtcgc
     6241 gcagcaccgg caccaccaag ccgttcggcg tgtccgccgc gaagccgatg ttgtagtact
     6301 gcttgaggac gaggttgtcg ccatccagcg aggcgttgaa gttcgggtac ttcttcagcg
     6361 cggccacgct ggccttgatc atgaaggcga gcatggtgac cttgccggcc ttgcccgcgg
     6421 cgatcgcctt cgcgttctcg ttgttgagcg cgacgcggag gtcttccagg tcggtgatgt
     6481 ccgcatcgtc gaactgggtg acgtgcggga tcatcgccca gttgcgcgcg aggttggcgc
     6541 cggaaatctt ctggatccgc gacagcggct tgctttcgat cgcgccgaac ttcgcgaagt
     6601 cgaccttcgg ccacgggatc aggttgaggc cgccaccgcc acccgcggac accgccgccg
     6661 gtgccgcgga cacgccgccg gccagcgccg acttcacgaa attctgcaca tcgagcttgc
     6721 cgatgcggcc gccgcgctcg ctgccggtca cctgcatcag gtcgacgccg agttcgcggg
     6781 cgaacaggcg gaccgccggg ctggcgtagg gcaccttgtc gggcagcacg gcgtcggcct
     6841 cgaagcgcac cggcggcagg ccggcgccca tcggcgcggc ggcgggcgcc ggtacgggcg
     6901 cgggaggcgc gaccacgacc ggctcgacct tggcgccggt ttcggatgcg ctggtcgttg
     6961 ccacggcggc gatcggctgg gcggcaggcg gagggggcag cacggacgcc ggtgcaggcg
     7021 cttcggcggc aacgccggca gcgacttcga tgatcgcgac cagcgcgcct tcggagaggt
     7081 tgtcgcccag cttgaccttc agttccctga ccaccccggc gaacggcgac ggcacttcca
     7141 tcgtcgcctt gtcggactcc agggtgacca gcccctggtc gacggcgacg gtgtcgccca
     7201 ccttgaccag cacttcgatg atcggcacgt cgtcatgccc cccgatatcg gggacgcgcg
     7261 cttccttgag gtcggccatg ccggtctccg ttttcgggtt gggaccgtat tgttggcaca
     7321 tcccgcgggc agcgacaaac cgcactgcgg caattttcct gttttacccg gctaattctt
     7381 tttttgtccc gaagttgctc gaaagctgcg tttttcgggc ccgcctacag ggtattcatc
     7441 ggggtggaat cagggatgct cggcctcggc gtgcaccttt tcgcgccgga tgaggtagag
     7501 gccactggcg atgatgatgg ccgcaccgag ccaggtgatc gcgtccggca gcacgcccca
     7561 caacgccagg tcgagggcga cgccccagac cagcgcggtg tattccagcg gtgcgatcag
     7621 cgaggcctcg cccaaccgga aggcttcagt gatcgcgtac tggccgatcg ccccggcgat
     7681 gccgatgccg ccgatgagcc acgcgtgttc cgcgcgcaac ggcacccagt ccggccaggc
     7741 cagcgcaccc gcgcccagac ccatcagcgc cagcaaccag aacgtgatcg cctgtgtgct
     7801 gtcggtacgg gcgagcacgc gcacggtgat cgccgacacc gcgtagccca gcgccgccac
     7861 cagcacggcg aaaccggcgg tggtgagcac gccttcgccg gtcgggcgca gcaccaccag
     7921 cacgcccagc aggccgatcg cgatcgcggt ccagcgccgc ggccccacgc gctcaccgag
     7981 gatcggcact gacaacgcgg tgatcaacag cggggcgacg aagaagatcg aatacgcggt
     8041 ggacagcggc aagcgcttca gcgcatacac gaagctgccc atcatcatga tgccgagcac
     8101 cccgcgcagc aggtgcagcg gccaacgcac ccgcaccagc gcgcgccagc ccgcagtcga
     8161 cagcgcccag gccagcacca gcggcagcga ggacatcgcg cgcaaggtcg ccacctgcag
     8221 gggcgggtaa tgcgcggaca gcgacttcaa gcccgcatcc atcagcgaga acagcaggat
     8281 cgacaccagc atcaggagca tgccgcggcg cgcgtggtcg tgtggggtcg ccggctgggg
     8341 atgcatccgg ccagcatgcg gtaacctgcc cgccccgtca cctgtgcgtc gccaccatgc
     8401 cgtcattcga catcgtttcc gaagtggaca cccacgaact caccaatgcc gtggaccagg
     8461 ccaaccgcga actgtccacg cgcttcgact tcaagggcgt ggtcgccagc ttcgaactgg
     8521 acgatgccgc catcaagcag tccgccccca gcgaattcca gttgcagcag atgaccgaca
     8581 tcctgcgtgc acgcctgatc gcgcggaaga tcgatgcgcg ctgcctggag ttcggcgaca
     8641 tcgagaccaa cctggccggc gcgcggcaga ccgtcacggt caagcagggc atcgagcgcg
     8701 aactggccaa gaaactccag gccgcgatga aggacgccaa gctcaaggtc gactcgcaga
     8761 tcaacggcga caagctgcgc gtcaccggca agaagcgcga cgacctgcag gcggcgatgg
     8821 cgctgctgcg tgccggcgag ttcggcgtgc cgctgcagtt cgacaacttt cgtgattgat
     8881 cggctcgcgc aagcgctcgc cggccgtgat tgactcgtgc aaacgccgtc gccgtgactg
     8941 acacattcga tcccgcgcag atcgaaaccg acgtccgtcg ctggctggaa cgcgcggtga
     9001 tcgggctgaa cctgtgcccg ttcgccaagg ccgtctacgt gaaagaccaa gtgcgcattg
     9061 tcatcagcga tgccagcacc gaacgcgcgc tgctggaaga actcggcgag gaactcgcgc
     9121 tgctgcgcga cacgcctgca gaacaggtcg acaccaccct gctggtgcat ccgcaggtac
     9181 tgggcgactt cctggactac aacgatttcc tcggcgacgc cgacgcgctg gtcgaggcga
     9241 tggacctgga cggcgtgctg caggtcgcca gcttccatcc gcagtaccag ttcgccggca
     9301 gccagcccga cgatgccgac aacctgacca atcgcgcccc gtatccgatc ctgcacctgc
     9361 tgcgcgaggc cagcatcgac cgtgcggtcg cggcctatcc ggacccggac gcgatcatcg
     9421 cgcgcaacat cgccacggtg cgcgaactcg gtgtcgaggg ctggagcaag ttgctcgcgg
     9481 agtgaggggt caggcctcgc tcaatcgcgc ttccagcagg cgttgcgcat cgcccagcga
     9541 cgccacgatg cgatggccaa gcgcacgcgc ggcgtcatcc ggctccagca ggtccggcac
     9601 ctgcaccggt gtcatcccgg ccgccagcgc agcgcgcacg ccggtcggcg aatcctccag
     9661 caccaggcaa cgcgccggcg cgatgccgag tttcgacgcg gcaagcaagt agacgtccgg
     9721 cgccggcttc ggcgcggcca cgtcgctgct ggtggccacc gcatggaaat agggcagcaa
     9781 ccccgccgcg gccagcttgc gcatcgccag cgggcgtcgg gtcgaggtcg ccaccgcacg
     9841 cggaaggcca cgtgcgacca gccagtcgag cagctcgacg atgcccgggc gatggtcgat
     9901 gccggcatcg gccacgacgt cgtacatcgc ttccgcacgc gccagcagtt ccatggtggc
     9961 atccgcgccg atgcgttcgg ccagcaggcg tcggcacagg ccatcgccgc cgccgatcat
    10021 ctgcagccac aggctggccg gcaggtcgtg gccgagatcg ctggccgcgc gcgccatgca
    10081 gtcgttgatc gcgcgttcgc tgtccagcat cagaccgtcc atgtcgaaca ccaccgcctc
    10141 cggcaggaac ggcagcggcg gaatcgcgac gggcgtcatg cgggttccgt ggcaaacagc
    10201 cggtcgcggt cggcggcacc cagcgcgcgc cactgccctt ccggcaagtc gcccagcgtc
    10261 agtccgccga tgcgttcgcg atgcaaggcc tgcacgtgat ggccgatcgc cgcgaacatc
    10321 cgccgcacct ggtggtagcg cccctcgtgc aggaccaggc gcgcgcgacg cgcctccagc
    10381 ggctcaagtt ccgccggcag cagcggcgtg gtttcgcttt ccagcatcag gctgccgctg
    10441 gcgaacgtcg cacgctcatc gccgcgcaag tcctcggcca atgtcgccac gtagaccttc
    10501 ggcagcttcg atttcggcga aatgatccgg tgcagcaact ggccgtcgtc ggtgaacagc
    10561 agcaggccgg aggtgtcgcg gtccagccga ccgacggtgg acaacgccgg attgcgctcg
    10621 cggaaccgcg ccggcagcag gtcgaacacc acgcggccgc gatccttcat cgagcatgtg
    10681 tagcccaccg gcttgttgag catgagcagc aggccgggtg gcggatccag cggctcgcca
    10741 tccacgcgca cgttgtcgtg gtcgagcgga tcgtccgcgt acagcacctc gccgtccgca
    10801 tcggtgatgc ggccctcgcg gaacatccac tgcacgtcct tgcggctgcc atagcccagg
    10861 ttggcgatgt gcttgaccag tttcattcga acctcgggca tgtcgatgcc atccatggcg
    10921 aaacgatctg gtctttggac gccaccgccc ggccatcaat ggccgtgcgt tcaaaggcgc
    10981 agcagccgcc ttgagggcgc ctgctaagca cgcgccttct caccttcgac gatcttgaag
    11041 ccgccgtcct gcgccagcgt ctgcacctgc gcgaagcctt cgcccagcgc gtgctcgtag
    11101 ggcaggtgcc ggttggcgac cagccacagg cggccgcccg gcttcagcgc ggttgctgct
    11161 gctgcaatga aggcacggcc gatgtcgggg cggtcgccgc gccccagcgc atggaagggc
    11221 gggttgcaga cgatggcatc gaagcgttcg gacacgccgg cggcgacatc gtgccaatgg
    11281 cgctgcacgg caacccgggc atccgcgagg ttggcttcgg ccaacgccag cgcgcgcgca
    11341 tcggcttcgt acaggtccag cgacgcgacc tgcgggcaac gcgccagcac ctgcatcgac
    11401 aggtagcccc agcccgcgcc cagatcggcc acgcggccgc cgaggtcatt cggcagcgcc
    11461 tgcgccagca tcgccgaagc ggcatccacg cggtcccagg cgaacacgcc gggccggctc
    11521 aggaaactgc cgccgggcag gttcgggctg atcaccctgc gcggcgcatc ggccttcgcc
    11581 cattgcgcca gcgtggcggg atcgaacatc gcatccggcc gtgtccagaa cacgcggcaa
    11641 tggtgcttgc tgatgacgct gaggttgccg gcgagttgct tgaggtcggc ctcgcgcgat
    11701 ttcgcgcctt cgtcgttggc gaccgacgcg accacgctgc cgcccggcgc cactgcgctg
    11761 caggccttgg ccagcagcgc ccgcgcttcc tcgcgctggc gcggcggcag gaccagcacc
    11821 agcggatagc gcgccgcggg caaggcgtct tcgtcgatga gtcctacgcc gatgcgctgc
    11881 aggcggtcgg cttccggctt gaacggctgg gtgcaggcca gggcctgcaa tccgccgcgc
    11941 gcatgcaggg ccgcgccttc gcgggcgcgc aggaacaggg cgtcgcccgc cggcggccag
    12001 cgcagggcgc cttcggcgaa gggatgcagg agggcgtcga gggcgatgtc gcgatgcatg
    12061 gaagccatgc gacaagtgta acgagccgac gccgagaatc aggcggcgct tgcctcgctg
    12121 gccagacgtc cgcgcaacag accgacgccc cagttgccaa gcacgcacag cgtggcgatg
    12181 ccgctccagg ccaccgcgtc ctggccgccc ggcaggactg tcgcgagttc ggccacccag
    12241 ccgcggccga accaggccac gaccactgcg gcggaaagcg cgaacaccag caccaggccg
    12301 cgcagcaggc gttgttccag caccgcgctg aaggtcgcct gggtgcgcgc cagcgcggcc
    12361 acccgtgacg cgaagtcgac cggcaagtcc accgacggcg catggcgaag cgccctggcg
    12421 atgcgcaggt cggtggcatc ggcgtcgggg tcaccgcggc gggcgcgctc ctgcgcttgc
    12481 caacgttgct cgtcaagggg gatttcgcgg tcgtggatgt tcatgcggcg actccgatgc
    12541 gcggcgcgag gatctcgcgc agtttgttgc ggctgcggaa caggtggctc ttgatggtgc
    12601 cttcggccag gccggtgatg gtcgcgatct cgccgatcgg cagttcttcc agatggtaaa
    12661 gcgtgaggat cgtgcgttgc agcggcggca ggcgctcgat cgcggcatgc agctcgcgct
    12721 cctcgccggc ctgcgcatgc gcctcctgca ggtcggtgcc gtcgccgaga ttgtcgagca
    12781 gggacggacc gtcgtcctcg tccatgcctg cgccgacatg cacatcggcc agcgcgatgc
    12841 gcttgcgctc caggtggcgc ttggccaccg aatacgccac ctggccgatc cacgatttca
    12901 gcgggctgtc gaagcggtac tggtgcaggt agcggtgcac gcgcaggaac gcctcctggc
    12961 acaactctcg ggtgtcctcg ggatgtcgca ccatccgctg caccacgtgc cagcacagcc
    13021 cctggtactc gcgcaccagc tgctcgaacg cgcccgcgcg gttcgcgagc acggcttcga
    13081 cgagggcgcg atcggcatcc atggcgcatg ggatacgccc ggggcggcat cggttgcaag
    13141 gacttgtcga cggatgtccc gctggcggct gcaaccgacc cagcgccggc cgtatctctt
    13201 tgcccgaacg gcccgcatgg ccggcccgac ccaccgagga catcccatga atttcgaact
    13261 gctcatcccg atcaccctgt tcgcctgcat cgcctattcg atcaaggcgg tggtcgacgc
    13321 ccgcgtgcgc aagcagctgg tgtccagcaa tggcgcaccc gacctggtcc gcagcatcct
    13381 ggagggcgac gaggtgaacc gccgcctgtc ctcgctgcgc tggggcatca ccctggtcgc
    13441 gctgtcgatc ggctttgcga tcgtcgaggc cgccggctgg cgcgagatca cccccggcgt
    13501 gatcgcggtg ctggtcggcg cggtcggcat cggcaacctg ggttccttcg ccgccgcccg
    13561 caagctcggc tgagcgggcc gcggcgggta tgctgtcggc ccccaaaggc cggcagcatc
    13621 ccgatgaccg acgcaagcaa cgccaccacc gacaccccgc ccgaccacct gtccaacgat
    13681 ccgcgcagca agttccatga cgcggccgtg ctggaacgtg gcatcggcat ccgcttcaac
    13741 ggcatggagc gcaacaacgt cgaggaatac tgcatcagcg aaggctgggt gcggatggcg
    13801 gcgggacgct ccctcgaccg tcgcgggcag ccgatgacga tgaagttcaa gggcacggtc
    13861 gaagcctggt tcctggacag cgtggcctcc gacgaaacgc cggctgactg aacgtcgccg
    13921 aaccgcgccc gccatcacgt tgcgggcgca aactcgtcgc gtccagccac cggagtcctg
    13981 ccatgaaccg cctgcgcacc ctgcccctgc tgctcgccac ggtcctcgcc ctgggtgcct
    14041 gcgcatccac cggcaacacc agcatgctcg cggccgatgc gccccgcgcc ctgcctgcgg
    14101 ccggcccggt cagcgtctcc tgggaagatc cggcacgatt caccgaggtg cgccacagcg
    14161 gcaaccgctg ggcggcaacc gaaggcaact ggctgaacac gctggccacc tacatgcgca
    14221 aggaagccga agaaaccctg cccgccggcc atcggctgga gttgaccatc gtcgacatca
    14281 agcgcgccgg ccagtacgag ccgtggctgg gcccgaacct gcaggacacc cgaatcatcc
    14341 gcgacatgta cccgccgcgc atgaccctgc gcttccgcga ggtggatgcc gacggtaccg
    14401 tgctggcgga aggcgagcgc aagctgtccg acccggcctt cctgatgaat gcctcgcgcg
    14461 tgaacgacac cgacccgctg cgctacgaga agcgcatggt cgacagctgg ctgcgcagcg
    14521 actggcgcac cgctgcgcgc tgacccatcc gaagcgatcc tgacgaagcc gccgcaaggc
    14581 ggctttgtcg tttcaggcgg cgtcgcaggc cgccgcgcgc gcaagcagca cggtgctttc
    14641 gcgggcgttt gcggtcagtg cggcggcacg ctcgaattcg acgcgtgcct catccaggcg
    14701 accgagttgt tgcagcaggt cgccgcgcac cgccggcagc ggcgcgtagt cgcgcagttt
    14761 cggttccgcg gccaggccct cgacaatggc caacgcggcc tgcggtccct gcgccttggc
    14821 caccgcaacc gcgcgattga gctcgaccac cggagacggt gcgacctgca tcagtcgtgc
    14881 gtacagcgcc gcgatcgcgg tccagtcggt gtcgtcgaag cgccgcgcgc gcgcatggca
    14941 ggcagccaac gcggcttgca acgcatacgg gccatcggca ccccccaggc gttgcgcacg
    15001 caccagcgcc tgcaggccac gctggatctg caggcgatcc cagcgcgacc ggttctgctg
    15061 gggcagcagg atcggcgtgc cgtccgcctg ggtacgcgcc ggcatgcgcg aggcctgcaa
    15121 ctccatcaat gccaacagcc ccaacgcttc ggtttcgttc ggcaacagcc cgaccagcac
    15181 gcggcccagc ctcagcgctt cctcgcacaa cgccgggcgc atccagtcat caccgctggt
    15241 cgccgcatag ccttcgttga agaccaggta gagcacttcc agcacggcat ccaggcgctc
    15301 cggcaactcg gccttgcgcg gcacctcgaa cggcacctgc ttctccgcaa gcgtgcgctt
    15361 ggcacggacg atgcgctgcg ccaccgtggg ctccggctgc aggaaggcgc gggcgatctc
    15421 cggcgtgctg agtccaccga gcatgcgcag ggtcagcgcg acccgcgact ccatcggcag
    15481 cagcgggtgg cagctggtga acagcaggcg cagcaggtcg tcgccgatgt cgtcgtccgc
    15541 cagccgttgc gcatcgtcgc cctgcatgtc ggctccgtgc aattccagtt cgacaccgta
    15601 ttcgtcctgc ttgcgcgcgt gcagctggcg ctggcgcacg tggtcgatgg cgcggtgctt
    15661 cgccgccgtc atcagccatg ccgcggggtt gtccggcacg ccctcgcgcg gccagcgctc
    15721 caacgcaacg accagcacgt cctgcgccag ctcctcggcg gtgtcgatgt tgccggtgat
    15781 gcgcgccagc cgggcgagca gcttcggcga ttccatccgc cacagggcgt cgaggcggcg
    15841 atggacggcg gcggcgttca cgccgccgat ctcagcatcg cccgccacca cgcgcaagtc
    15901 gtgctcaacc catcgaatgc aggccgaaca tgttgccttc ggtgtcgaag gccagggcga
    15961 tgctgccgta ctccccgatc gacatcttgg cctggtgcag cctgccgcca gccgcttcga
    16021 cgcgcccggc ttccaccgcg caatcggcgc agctgaaata gaccagggtg ccgcccacgc
    16081 cgggcgagac cccttccatc ttgcacaagg tgccgctcgc gccgtggctc tccatgttcg
    16141 cggggaaggc cagcatctgg aagccgtcca tgccgccggg catcggcaac ggttccaggg
    16201 tcacgccgag cacggcttcg tagaaggcgc gcgcgcgggc caggtcgtcg gtgtagatct
    16261 cgaaccagac caccgggttg gatgcattca tcggattgct ccttgtgcag ggcgcggcaa
    16321 cgcttgccgc gccacatgtg atgcgcctgg ttcaggcgcc ggggaactgc gattcgtcca
    16381 tgaagaacac ttcccactgg tgtccgtcga ggtcggcgat gccgcgctgg tacatgaagc
    16441 ccaggtcgcg cggctcggcg tactcgacgg cgcccttggc gatggccctg gccacgaagt
    16501 cgtcgacctc ggcgcgactg tccagcgaga gcgccagcaa ggtttcggtc acggccttcg
    16561 catcggcgat gggcttcctg gtgaaggtcg cgaagaacga ttcgaccagc agcatcacgc
    16621 tggtgttgtc gttgacgatc atgcaggcgg cgttctcgtc ggtgaacatc gggttgaacg
    16681 caaaaccgag cacggtgaag aactcgacgg tgcgatcgag atccttcacc ggcaggttga
    16741 cgtagaggtt gcggggcatc ggatcgtcct tggcctggcg cgcgctcagg gcgccggctt
    16801 catcaggttg atcatccacg gctggccgta gcggtcgtcg aacatgccga agcgcagcgc
    16861 ccactggctt tcggccagcg gcatccgcac ctggccgccg tccgacatcg cggcgaagat
    16921 gcgctcggcg tcctcgatgc tgtccacctc gatgttgacc gaggtcgtgg cctggccctc
    16981 tggattctcc atcgcatccg cgcccatgat catggcgccg ccggcgagca actggatgtt
    17041 ggcgatgaag ggccgcatcg gctccgggat gtccggcccc atgtcgccat acgtcgcggt
    17101 cgagacgatc tcgccaccca gggcccttgc gtagaacgcg aaggcctcct ggcattttcc
    17161 ctcgcggaac atcaggtacg gaatcagttt catcgggtcg ctcctgtcac gtgtcgggtc
    17221 agttgccgag gcgctcgcgc aggcggtctt cctgttcgcg cagctcgggg gtgaattcgg
    17281 taccgaagtc ctcggcttcg tagagggggc ggatttccag ttcggacgga ccgggcatcg
    17341 gattcgggca acgcttggcc cactcgatcg cttcgtccat cgaacgcacc tgccacagcc
    17401 agaagccggc gaccagctcg cgggtctccg cgaacgggcc gtcgatcacc cgccgcgacg
    17461 ggccatcgaa ggcgatccgc gtgccgtgcg aagtcggctt caatccctcg ccagccagca
    17521 tgaccccggc attgaccagc tcctcgttga acttgcccat ggcttcgaac agctcggcag
    17581 tcggcatgac gccggcttcg gacgcatcgg tggccttcac cagcaccatc actttcatct
    17641 cgcgctccct ggttggatcg ggtgtgactc cgcccttcct gcaacacgac ggacgatggc
    17701 gaccgcgatc gacacgccgt cgaaaaaatt tcctgtcatc cccaacgaag cgggcacggt
    17761 ggacggccgc tcgcattcag gttgtcgcct gccacctcgc cctgcatcat cccccgaccg
    17821 cgccacgatc cggagcccgc gatgcaattc ctgaccctga tctacatcga cccggaacgc
    17881 ctggccgaat tgccggcgga ggagtacgac cggttgatgc gcggctgctt cgagaaggcg
    17941 gatgcgctgc gcgatgccgg ctgcctgcat ggctcgcagc aactgcagga tccgggcagc
    18001 gcgcgcacgc tgcgtgtccg cgatggggtg tcgcggatca ccgacggccc gttcgcggaa
    18061 acccgcgagt tcctggccgg cttcaacctg gtcgaagcgg ccgatatcga ggccgcgatg
    18121 gagatcgccc gcgcgttccc atggtcgcag ttcggcagca tcgaagtccg cccggtgcgc
    18181 gacatgcagg cggtgcgcgt gcaggtcggc gcccactgac gacgcgggaa accctgaccc
    18241 acgcgcgggt tgccggccga cggtcgttcg accctcgccg cacgaagctg gccacgtctc
    18301 caacgccgac gacgtgccat ggacctgcgc cccgccctgc ttgccctgct gctgctcgcc
    18361 tcgcaaccgg ccgcgcacgc cgcctcgccc ctccgagcgg cgcccgcccc gatgctcgcc
    18421 agccggatca ccgacgacat cgacatcgcc gcctggctgg tcagcgagaa actcgacggc
    18481 gtgcgcgcac gctgggacgg ccggcagctg gtcacccgtg ccggcaatcg catcgatgca
    18541 cccgcctggt tcaccgccgg ctggccggct caggcgatcg acggcgagct ttggatcggg
    18601 cgtggacgct tccaggacat cagtgacctg gtccgcgccc tgcgcggcga cgcggcgggc
    18661 tggcggcagg tccgcttcat ggccttcgac ctgccggcat cgccgcatgc cttcgccagc
    18721 cgcacgcggg aattgcgcgt cctggtggaa gagacagcat cgccgcgcct gcagcgcatc
    18781 gcccagctcc atttcagcga gcgcgcgcaa ctggacgcgt ggctgcgctc ggtggcgagt
    18841 ggtggtggcg agggcctgat gctgcaccac gccaacgcgc attaccgcgc cgggcgcagc
    18901 gaccagttgc tgaagtacaa gcgctgggac gatgccgagg cccgcgtggt cggctaccgc
    18961 ccgggcaagg gcaagtacca cgggatggtc ggcgcgttgc tggtggagga cgcgcatgga
    19021 cgccgcttcg cgctgggcag cggcctgcac gatgccgaac gcgccgcgcc acccgcgatc
    19081 ggcagcacgg tcacgttccg ctacgacggg ctgaccgcga agggcacccc gcgattcgcg
    19141 cgctacctgc gggtacgccg cgagtgagca tgctccgtgg cgcctgcggc tactccggct
    19201 tgcggtaaag atgcacgaag cgatcggtcc tgccgcggat cgccgggtcg aacacgttct
    19261 tgctgcggtc atcatcggga ttccgcagga ccgggatcgc cgcttcccac tcgagcccgt
    19321 gcttgcggaa gtcggcgatg gcgaactgct cgtcgatgcg gtgcagggcc tgcgcatcgg
    19381 cgctcccggt accctgcctc gcgctgtggt cgaccaccag cagcacaccg cctggcttca
    19441 gcgcacgtgc gacctggtcg aggaacaggc ctgcatcgac cctcggccag ccctccttct
    19501 cgtcgaccca gtacaggtcg tggtaggaca tcacgatcac cgcgccatcc agcacgcggt
    19561 cgcccatgcc cagcgcgtcg ctcggcccga ccacatgggt cagattcggg agccgattgg
    19621 cggcgaggcg ctcggccagg cccttcttgc cgaaattctc gtaacccggg ttgttgacca
    19681 ggagcacctt gccgccgggt ccgacgatgc cggacaggat ctcggcgtag taacccccgc
    19741 ccgagaggat gtccgccacg gtgtcaccgc gcttgaagcg ggccagggcc agcacctcgg
    19801 ccggcttgtc gcgggcatcg cgctcgcggt ccgaggcgct gcgcgcgggg ttggccagga
    19861 cgcgttcgat gcctgcggat tcgttgatgc tgacgccggc acaaccggac aggagggcaa
    19921 cggcaacaca ggacgcgagc agcaaggtgc gcatggcgga actccgtgga caatcaggcg
    19981 atgaccgcat catgcgcgcc tgcaccgcgc gtgcgcgggc cggaggtcat gccccaccca
    20041 aaacgggtca acccacgtac ttcgccacca cgcagttgcg gccgcgcgcc ttggccgcgt
    20101 acatcgcccg gtcggcggcc aggaggaggc catcaggctc gccgccctcg gagggccagg
    20161 tcgccgcggc gccgatgctg acggtcacat agggtgcgat cggcgaggcc ggatgcgcca
    20221 gccgctcgcg ggccaccgcc ttgcgcagcg cctcgcccac cgccaccgcc gcttccaggt
    20281 cgcggcccgg cagcagcagc acgaattcct cgccgccgaa gcgcgcgacc aggtcgtcct
    20341 cgtcgcgcag ctgcgccgcg cacagccggg ccagcgcccg caggcattca tcgccatgca
    20401 ggtgcccggc ggtgtcgttg agcgccttga accagtcggc atccgccatt agcaaggcca
    20461 gcggcacgct gcccttgacc aaccgcgccc acacctgctg cagggccgtg tcgaactggc
    20521 ggcggttggc gatgccggtc aggccgtcct gcatcgacaa tgcctgcagc ttgcggttgg
    20581 ccacttccag ctcgcgcacc acccgcgcca ggtggatggt gccggcgacc tgcgcggcga
    20641 tcgcatcgaa cacctcgcag gcttccgcgg agaagaaatc cgactgcgtg ctctcgatgt
    20701 tgagcacgcc atgcaggcgg ccacgatggc ggatcggcac caggtattcg gaccgcactt
    20761 cgctgttgcc ggccaggtaa tccgggtcga ggtcgacatt cgcgatcagc tgggccacgc
    20821 cagtgcgtgc gcaacgcccg gctgccccct tgctgaccgg ccagtcatcg gcgggctgca
    20881 gcacgttgag ggtgagctcg ccggcccaga cctcgtgcac gaaatgcgtg cacgcctcgt
    20941 tgagcaggac gatgctggca agcgccaccg gcatgcgctc gaccaggcag tccacgatcc
    21001 gccgcaacac cgcttccagg tcttcgcctt ctgcggcttc gcgcgagacc cgcgccagca
    21061 gctcggtcag gcgtgcacgc cggtcgcgcg cctcctgctc ggcccagggc aggccgggtg
    21121 cgtcggtgcg caggttgtcg atcgccggct gtgacatgcc acccctcgtg cggcagtcta
    21181 gcgcgaagcg tccagccgcc cgtgcctgcg gctggaccgc ccatcgccac gccggaccgg
    21241 gacgcggcca acggccgatg ccgccagcct ccggcttcgt cgccacggac gcgggttccg
    21301 tcgcagtgcg gcttccatcc ccgcgatgcc ccactatcct cgcggcactc cttcaacgac
    21361 caacgcccac catgttcaaa agcatcggct tcgtcctccg catcgccatc gcctggggcc
    21421 tggtggtgtt ggtggttgcc tcgttgtatt cgtcgatccc gctgcttggg cgcttcgagt
    21481 tgccgatcgt gttggtgtcg atggcaacga tggcgctggt cgtcggcggt gcgttctcgc
    21541 acctgcgccg cgtgcgcctg atcgcaggcc acatcgatgg cgacgccctc ggcaaccgcc
    21601 agaagcgact ggtcgagatc ccgctggagg ccggcgaggc cttcgacctg ctggacgccg
    21661 cgatccgcga actgcccggt gccgaacaga tccagagcgc gcgcgacagc ctgcaggtga
    21721 aggccaaggt ggcgcggccg cgcacctatg gcgaacaccc gctgcggcgc tggaacccgt
    21781 tgctgtggtt cggcctgccg cgcaaccagt tgcaggccac ggtcacgccg gccggggaca
    21841 gcggtcgggt caccctgatc tgcgaacccg agaatccggc gtggagcgac tggttcctgg
    21901 tcgatgacgg caccaactac gagaacgccg aggccatcgt ccgcgcgatc acccgccgca
    21961 tcggcgagcg ccgccgcggc gagcaggcca gcgccgcgca gaccgcaacc gagaaggaac
    22021 tgaccgaggc caagctcaac ctgctgcacg cgcaggtcga gccgcacttc ctctacaaca
    22081 cgctggccag cgcgcaactg ctgacccgca gcgatccggt ccgtgccgag gcgatgctcg
    22141 gccatctcat ccagtacctg cgccgctcgc tgccgaatgc agaaggcgag atgtccacgc
    22201 tcggcgcgga actcgaacgc gcgctcgcct acctggagat cctcaaggtg cggatgggcg
    22261 accgcctcga cgtgcagacc gacgtggcag aagccctgcg caatacgccg atgccggcga
    22321 tgatgctgca gacgctggtg gagaacgcga tcaagcacgg cctggagccg cgcaccggcg
    22381 gcggcaccgt gtggatccgc gcacgccgcg acgacaacac cgtggcggtc accgtggccg
    22441 acaacggcga aggcttcaac agcaagacca gcggcaccgg gatcggcctg aaaaacgtgc
    22501 gcgagcgcct gcgcctgcgc tatgccggcg aagccaacct ggccgtggtc gccaatttcc
    22561 ccgccggcgt ggccgccacg ctgaccgtgc cggccaatgg acgcaacgca catggctgac
    22621 atccgcacct gcatcgtggc cgaggacgag gcgctgctgc gccacgccct ggtcgccgaa
    22681 ctccagcgcg cgtggccatc gctgcgcatc gtcgccgaat gcgaagacgg cgccagcgcg
    22741 gtggaagccc tggccgagca ccagccggac gtcgccttcc tggacatccg catgcccggc
    22801 ctgaccggcc tggaagtcgc caccaccgcc gccgaggcga gtccgcgcac gcgcatcgtc
    22861 ttcgtcaccg cctatgacca gtacgcgatc gatgccttcg accgcggcgc catcgactac
    22921 ctgctcaagc cggtcaagcc ggaacggctg gacgccacgg tggcgcggct gcaggcgcag
    22981 gacaccctgc cggatgcggc gacattggcc gccctgctcg acaagctcgg cgcgatgccg
    23041 gacccggcgg gttcgccgga accgctgacc tggctgaccg ccagcgccgg tcgcgaaacg
    23101 cgcctggtgc tggtcgacga ggtcatctat ttccacgccg accagaagta caccaccgtg
    23161 gtcaccgccg acggcgaagc gctgctgcgc acgccgctgc gcgaactcct gccccggctc
    23221 gacccggcca cgttcaagca gatccaccgc tcgaccatcg tcaacctgaa ggcagtcgcc
    23281 gggatcctcc gcgacgacag cggacgcggc acgttgcggc tgaagcaccg cccggaaacg
    23341 ctggcggtca gccagccatt catggccctg ttcaagcaga tgtaaaccgt cggttcgacg
    23401 gcagtcgtca ctccgcgcgc gccgcgcgcc atcgacacga ggcatcccat gtccaagttc
    23461 atcgcgttgc tgcagttcct gctcgccctg tccggctgct cgattggcgg caccacgtac
    23521 accagccgca tcgacagcga ccaggtgcac ctgaccagca aggcgcatgt gcaggacggc
    23581 gtggcccgat tcgattgcgt cgagagcagc agcggcatct gccactacac gctctatccc
    23641 gaggcgtgct cgggcaaggc cgactgccag ctggccccac tgcaacgctt cagcgtgccc
    23701 agcggcgaaa gccaccaggc ggccggcctg atcgacttcc gcccgtgcgt ggcgaccacc
    23761 gccgcggcac tcggtgcgga ctgccagcct gcgactgcgg gcaaggcacg ctgatggcgc
    23821 tgcggcgatt cctgcgcttg gcaccgaagg tgatgctggg cctggtgctg cttgccatcg
    23881 tgggcgtcgc cgtgctgctc ggcccttacg catggcgggt agagcacatc gccgcggacg
    23941 cacgcgtcgg cttccgttcc gattactacc tgtacgtgcc ggagacgctg catcgcgacg
    24001 ccaacggcaa ggcggtcctg ctggtgcagc cgaacaattc cgggcgcacc tcggatgaca
    24061 tcggcctgca ccgccgcgat gcatggatga ccggcttcga gcgcaagcgc atcgcggacg
    24121 aactgggcgt ggcgttgctg gtgccggcgt ttccgcgcac cgccagcgac tggaaggtgt
    24181 acacgcatgc gctcgaccgc gacgtgctga ccacccgtga gcctttgctc gaaaggctcg
    24241 acctgcaact gatcgcgatg atcgatgatg cccgcgtacg cctgcggaac gccggcacgc
    24301 cggtcgatga gaaagtgctg ttgcagggct tctccgccag cgcgatgttc gccaatcgct
    24361 tcaccgcctt gcacccggat cgcgtgcgcg cggcgagcat cggttcgccc ggcggctggc
    24421 ctatcgcgcc ggtggcgcag gtcggcgtgg atgccctgcc gtatccggcc ggcgtcgccg
    24481 acctgtccgc actgaccggg aggccgttcg atgccgccgc gttcgcgcgc gtgccgcagt
    24541 atctctacat gggcgacgcc gacgataacg actcgctgga tttcgaggac ggctgggaca
    24601 cgccgatgtc cgcggaagtg gaccgcctgt tcggcgacac cccgctcgcc cgctggacgc
    24661 atgccgaagc gctgtacgca cgagcaggcg cggatgcgcg tttcgaactg gtacccggca
    24721 tcggccatga ccgcaaggcg ttgcaggagc gttccaccga attcttccag cgcgtactaa
    24781 ccacgccacg ctgaaggccg gatcgccgcg ggccgttaac atcggccgat gaactacctc
    24841 gcccacgcct ggctggcacg ccattcggac gacgccatcc tcggcgcgat cctcggcgat
    24901 ttcgtgttcg gccaatccgc actccaggac tggccaccgg ccgtgcgcgc ggagatcgtc
    24961 cgccatcgcc gcatcgacca gtacaccgat gcgcacccgg cggtggtcgc agcgcgcggg
    25021 ctgttcgatg ccgccggcct gcgtcgctat gccggcatcg tgctcgacgt gtatttcgac
    25081 cattgcctgg cgcgcggctg ggtacgctgg aacgatgcac cgctcggcga tttcactgcg
    25141 cgcgtctacc gcgtgctgga cgcccatcgc gaagaacttc cgccgcgcct gcaggcgatc
    25201 gccccgcgca tggccgcgca cgactggctg ggctcgtatg cgcaacgtgg caacgtggac
    25261 cacgcggtgc gcggcatcgc cacccggctg tcgcgcaatg gcgacaagct ggtggcctgc
    25321 ctggacgtgc tgcgcgtcga ggaaacagcg atcgatgccg ccttcggggc gttcttcccg
    25381 gacctggtcc atgccgcggc gcggatgcgc ccttagactc gccgctgacc atcgcgccag
    25441 gagggcgccc atgcggacac gcattctcgg attgctggcg ttagtgctgc tcatcccggc
    25501 cgcccacgcc caggtgaagc tggatcctta cctgaagcag gatggctacg ggcgcatcaa
    25561 gatctcgccg gacggccagt actacgcggc caccgtgcag ctcgaggaca gcgtgggcct
    25621 ggccctgctt cggcgctcgg acaagaagat cgtcaatggc gccagcggga tcaaggattc
    25681 ggtggttgcc gatttctggt gggtcaacga ccaccaggtg gtgatcgcca tggccgagcg
    25741 cttcggcagc cgcgaccagc cctatcggac cgggcaactg ttctcgatcg gggtggacgg
    25801 caagcgcggc aagcagctgg tcgggcccaa gccgcaaccc gggctggtca ccgtgatcgg
    25861 taacgccggc ccatgggaaa tgaccgacct gatcgattcg ctgccggggg atgacgagaa
    25921 cgtgctcatc tccgcctgga agctgggcac caatccgttg accacggtcg acctgatgga
    25981 cgtgaacaac ggccggcgca agcgcatcgc caccgcgccg gtgaaccgcg ccgtgttcct
    26041 gaccgatgcc cagggccagg tccgcttcgc gcagggcgcg cagaacgaca attacagcaa
    26101 gctctactac cgcgacgaca accagtccga atggcggctg gtcaacgacg aaagcagcag
    26161 ccggcgcgtg gacaccccgg tcggtttttc cgccgatggg cgcatcgcct acttcaattc
    26221 atcgacggag gacggcccgg gtgcgctggt ggcctgggac atcgccaccg acgaccgcaa
    26281 gcaggtgttg cgcgatgcca ccgtggatcc ctacgcgatc atccatgacc gcgacgggca
    26341 caccgtgctg ggcgtgcagt tcatgtcagg gggcgtgcag acccgcatgc tggacgacag
    26401 tgcgcccgcc tcgcgggtgt atcgcgcgct ggccaaggcc tttccggacc acgccgtgaa
    26461 cgtcacctcg catacccgcg acggcgtggc cctggtgcag gcctggaacg accgcacccc
    26521 gggcgacacc tacctcttcg accccgcgaa gatgtcggcg aaggacgtgt tcatccagcg
    26581 cgagtggttc gatccggcca agctgccgaa ggcacgtgcg atcacggtca aggcccgcga
    26641 cggcctggtc ctgcatggct acctgaccct gccgttcggc gcgaaggaaa cgaccccgtt
    26701 gccgatggtg gtcctgccgc acggcgggcc gttcggcatc ttcgaccaat gggcgttcga
    26761 cgacgacacc cagctgctgg ccgaggccgg ctacgccgtg ctgcggatca atttccgcgg
    26821 ctctggcaat tacggccggg cgttccacca ggccggcgca cgcgagtggg gcggcgcgat
    26881 gcaggacgac ctgaccgacg ccacccgctg ggcgatcgcc gaacgcatcg cagatcccgc
    26941 acgcatctgc atctatggcg ccagctatgg cggctacgcc gccctgatgg gcgtggcgaa
    27001 ggaacccgac ctgtatcgct gcgcggtggg gtatgtcggc gtgtacgacc tggaagccaa
    27061 gcatcgcgag gattcgcgcg atgccaagtg ggtccgcaac tggatggacg actgggtggg
    27121 agaacgcagc acgctggcgg cgcggtcacc caccgaaatg gcctcgcgga tcaaggtgcc
    27181 ggtgctgctg gcggcgggcg gcgccgacgt gatcgcgccg atctcgcaca gcaaggcgat
    27241 ggcacgcgcc ctgcgcgatg ccggcaagcc ggtccagacc ctgtacttcg acagcgaggg
    27301 ccatggcttc tacaccgagg accaccgtcg agccttctac acgacgctgc tggacttcct
    27361 ggccgacaac atcgggggta cacgtgccgc caaggcggcc ggggagagca gcccgggcgg
    27421 tacgtgaccc gaaaaagcct tgggggtttg ccccaggtgc cgcacggatg cgaaccgatg
    27481 gggcggtcgg tccgcatccg cacgacgagg aagcgtggcc tggcgggata accggccgag
    27541 tcgcgcgggg agtcgcgacc cggccggcgg ccctggaact cagcggcagc ggaagtacgt
    27601 ggcctgcggg gtggtgtagc tgcggttgct cgcgtacccg ctgctgttgc cgtagccaac
    27661 gccgaagtcg cgttcgcggt ggatgcgggc cggctctgct ccgccgtccc cgcggcgcgg
    27721 ggtcaggcgg cgctcgacat aggcggccgg ggtgggggac tgggtgcgga acatgaacgt
    27781 ctcctgtact ggtttgctgg tgtgctggta atgtaaaaca ctaaatcaat gcagcgcatg
    27841 ggcaggaagt catgcgtgtc gcaatcgatg ggaatcgttc agctaacctg cccgcaccct
    27901 gcaggagaca gagatgccga aagcggccgc cgcgttgcaa cgctgtttct ggagcgacgg
    27961 ctccgatctt tacgacggct accacgacca cgaatgggga cgtcccgtca ccgacgacgt
    28021 gcgcctgttc gagaagatca gcctggaagg gttccaggcc ggcctgagct ggatcaccat
    28081 cctcaagaag cgcgacaact tccgtgccgg cttcgacggc ttcgatttcc acaaggtcgc
    28141 gaggtacacg gaacgcgacg tggagcgcct gctgggcgat gccggcatcg tccgccaccg
    28201 cggcaagatc gaggcggcga tccacaacgc cggccgcgcg atcgagttgc agcgcgagtt
    28261 cggttcgctg gcgcgctatt tctggcagta cgaagtcgac acccgcaagc gcccgaagaa
    28321 actcaccgtc gacgtgctgc gcaccttcac caccgcaccg gaagccattg cgctgtcgaa
    28381 ggacctgaag aagcgcggct ggaagttcgt gggcccgacc acgatgtacg ccttcatgca
    28441 ggcgatgggc ctggtcaacg accaccagca cgattgccat gtacgcggcg aggtcgaggc
    28501 gaagcggaaa gccttgcaac gcccctgatg catccacccc ggcatcacgc gcgtatgccc
    28561 aaagtcgcca cccaacctct cgaccaagga gcctttgcca tgcgcctgct cgtgctgctc
    28621 gccgccatcg ccatgcccgt cgtggcctgg ctcaccaaca gcggcacctt cggccccgat
    28681 aacgggacga tgtcggatcg ctacccgacc ctgctggtcg ccgccggtta cgcgttcgcg
    28741 atctgggggc tgatcttcct gctcgacctc gcctacgcgg ggtggcaact caccggcacc
    28801 cgcaagagcg acgacaccct gacccgcatc gcgccatggg ccgccggcgg tttcgcgctg
    28861 accacgctgt ggatgccgct gttcgccatg tcgcagttct ggctgtgcct gctggtgatc
    28921 ttcggcgcca ccttctgcct gatccgctgc gcgatgatcc tgtcgcacga ccgcacgccg
    28981 caggaaggcc agtggatgtg ggcgtggctg ccactgtcgc tgcatgccgg ctggctgtcg
    29041 ctggccgcct tcctcaacct ggcgcaggtg gtggtggcct acgaactggc ctcaaccacc
    29101 cagcaactgc cgtggagcct ggcgatcttc gggctggccg cgctgatgct gctgatggtc
    29161 aacctgcgca tgcgcggcaa cgtggactac gtggtcgccg cgctgtgggc gctggcggcg
    29221 gtgtacgtga agcagtccgg caacgacatg cccggcgcgc agaccgccgc atgggtcgcg
    29281 gtcgggatcg cggtggtgct ggtgctgcag accgtgatgc tgcgccgcaa gtacccgggc
    29341 ggattgctgc ccaatccggc acgcgcgccg ggctgaggta ccgcaagacc gcgtgacagc
    29401 gaggccggcg tttgccggtc tcgccttgtg cgacccatgg gtgtacaaat gtacaccatg
    29461 gatgccatcg accacctctc gccgcaacgc gccgccgtcc tcgccttcct gcagcgggag
    29521 ctggtggccg gccgctcccc aagcttggcg gaaatcgccc tggccttcgg attcgcctcc
    29581 cgcaacgccg cgcagaaaca cgtgcaggca ctggtcgccg acggcctgct ggagcaggcc
    29641 acggggcgct cgcgcgggct gcgactgccg ggcgggccgg cggacctgtt gccgctggcg
    29701 gtactgggcc gggtggccgc gggccagccg ctcggcgcgg acatcggcct ggaccagcag
    29761 ctgtggctgg atcgccggct gttctcgccc accccggatt acctgctgaa ggtgcagggc
    29821 gattcgatga tcgacgacgg catcctcgaa ggcgacctgg tcggcgtgca gcgcagcgcc
    29881 gatgcgcgcg acggccagac ggtcatcgcc cgcatcgatg gcgagctgac catcaagcgc
    29941 ctgcagcgca gcgcccgacg gctgcgcctg ctcccgcgca atcccgcgca tgcgccgatc
    30001 gagatcgaac gcgggcagga cttcgcgatc gaaggcgtgt actgcggcct gctgcggcag
    30061 ggctgagatg caggccgtcg cctccctcga ttcgctgctg gccgcgcaga cgctctggca
    30121 tgccgggcgt catccggcga ccacccgcga cggcgaaccg accgggcatg ccgcgctcga
    30181 tgccctgctg ccgcaacgcg gctggccgcg acgcgcattg accgaactgt tgctgccggc
    30241 ggacggcgtc ggcgaactgg ccctgctgat gccgacgctg gcacggctca gccgcgaggg
    30301 cggcacgctg gcgttggtcg ccccgcccta cctgccgtat gcgcctgcgt ggcaggcggc
    30361 cggcatcgac ctcggcgcat tgcaggtcat cgaaaccacg ccgcgcgatg cgctgtgggc
    30421 gttcgagcaa tgcctgcgca gcggtgcctg cgcggccgtg gtcggctggc cacggcaggc
    30481 ggatgcgcag gccttgcgcc gcctgcaggt ggcggcggac agcggcgact gcctcggctt
    30541 cgtgctgcgc gaccgcaagc acgcggcgaa tccgtcgccg gccgcgcttc ggctggaaca
    30601 cgacagcgat ggctggcgcg tgcgcaaatg ccgcggcggc ccggctccca cgcgcgcgtt
    30661 cgcgccgggc ctgcattgag ccgcgcgcat gttgtgggcc tgcatcgtgc tgccgcagct
    30721 ggcgctggac gccgtcctcc ggcgtttgcc tgatcccggc gcgccgctgg cgctggccag
    30781 cggacccacg caactgcgca ccctgcatgc ggtgaatgcc ggcgcggcgc aggccgggct
    30841 gcgcgccggc atgcgcctgg ccgcggcgca tgcgctgctg cccacgctgg cgacggtcga
    30901 gcatgacccg caggccgaag cgcgctggca gcagttcctc gccgcgtggg cgtatcgcca
    30961 cagttcgctg gtcagcgcgc agtgggccag ctgcatcgtg ctggaagtgc gcgccagttt
    31021 ccgcctgctc gggccgtggc cgcgcatcga agcgcgcctg cgcgaggaac tggccgcgct
    31081 cggcttcgcc caccgcatcg cgatggcgcc gacgccgcgc gccgcgcgcg tgctggccgg
    31141 cttgcaggat gggctggcgg tgtccaatcc ggcggcgatg caggcgctgc tggatcgcgt
    31201 gccggtgcgt cgcgcggtgc tgccgggcga taccggcgaa cgcctgcagc ggatgggcat
    31261 ccgcgacctg cgcaccctgc gcgaattgcc gcgcgagagc ctgcgccggc gcttcggtgc
    31321 ggaactgctg ccgcacctcg accgcctgta cggcgatgcc gacgatgcgt tgagctacta
    31381 cgcgccgccg gatcatttcg atgcgcgcat cgaactgggc cacgaggtcg aaagccacac
    31441 cgccctgctg ttcccgctgc gccggctgat cggcgacctg tgcacctgcc tgtcgatccg
    31501 cgatggcggc gtgcagcgct tcgtcctgcg tctggagcat gacgtccaca ggcacgacac
    31561 gcgcgcgcat accgatgtcg aggtcggcct gctcgccgcc gaacgcgaac ccgcgatgct
    31621 gttcgaagtc acccgcaacc ggctggaacg cgtgtcgatc ccgaagccgg tggtcgcgct
    31681 gcgcctgctc gcgcgcgagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    31801 acgcacgccg cgattactac gtgctggaaa ccgcgcaggg ccagcgcgca tgggcgttca
    31861 ccccggccgg tgaacgcgat ggctggatgc tgcacggctg gtttgcatga gtgacgcccg
    31921 gtgagctggg acgatgccgt cgatggcgtg gaccgcgaga ccccgggcgg gcgcatgccg
    31981 cgcgcatggc aggtggcgca gcgccgcgcg ctccctgcgg ccaacgacga cgcgcccggg
    32041 cacgacgacc tgcctgccta cgcggagctg cattgcctgt cggacttctc gttcctgcgc
    32101 ggcgcggcga gcgcggaaga gctgttcgag cgcgcgcacc gttgcggcta cgaggcgctg
    32161 gcgatcaccg acgaatgctc gctggccggg atcgtgcgcg ggctggaggc gtcgcgcgcc
    32221 accggcttga agctgatcgt cggcagcgag ttcgtcctga gctgcggcat gaagttcgtg
    32281 ctgctggtcg aacatgccgc cggctacacg cagctgtgcc ggctgatcac ccacgcgcgc
    32341 cgcgccgcgc cgaagggcgg ttacctgctc acgcgcgggg atgtcgaaac cgtgctggcc
    32401 gatgccgacg tcgggctatt cgcgctgtgg tgtcccgctg cgcagcccga tgccaatgaa
    32461 ggccgctggt tgcacgcggt gttcggcgag cgcgcgcatc tcgcagtgga gctgcatcgc
    32521 gaacaggacg acgaggcccg cctgcacgcc ttgctcgccc tcgcgcatga cttgcagctc
    32581 atcccgctgg ccgcaggcga cgtacacatg gacgtgcgcc gccagcgcgt gctgcaggac
    32641 acgatgaccg cgatccgcca tgggatgacg ctggccgact gcggcgaaca cctgttccgc
    32701 aatggcgagc gccacctgcg cacgcggcgc gcgctcggca acatccatcc ccatcacttg
    32761 ctcgacaacg ccgtgcaact cgcgcggcgc tgccgcttcg acctcggcaa cgacctcgaa
    32821 taccactatc ccgccgaact ggtgccaccc ggccacacgc ccaccagctg gctgcgtgca
    32881 cgcgcggagc aaggaatgcg tgagcgttgg cccgaaggcg tgccggcgaa gatcgtcgcg
    32941 cagatcgatg acgaactcgc gctgatcgaa tatctgaaat acgaagcgtt cttcctgacc
    33001 gtggacgaca tcgtccgctt cgccaaatcg cgcgagatcc tctgccaggg ccgcggctcg
    33061 gcggcgaatt cggcggtgtg cttcgcgctg ggcatcaccg tggtgaatcc cgccgagagc
    33121 cggctgctga tggcgcgctt cctgtcgaag gaacgcaacg agccgccgga catcgacgtc
    33181 gacttcgaac acgaacggcg cgaggaagtc ctgcagtacg tgtacgcgaa gtacggccgc
    33241 gaacgcgccg cgctggccgc cacggtgatc cgctatcgcg gcaagagcgc agtgcgcgac
    33301 gtggccaagg tcttcggcct gccgccggac cagctggcgc tgctggccga atgctacggc
    33361 tggggcaatg gcgatgcgcc gatgcagcaa cgcctgttcg aggccggctt cgatcccgac
    33421 aacccgctga tcgcgcgcgt gctggcggtg accgagcaat tgcagggcca tccgcgccac
    33481 ctgtcgcagc acgtgggcgg cttcgtgatc agcgatgcgc cgctgcacac ggtggtgccg
    33541 gtggagaacg cggcgatggc ggaccgcacc atcatccagt gggacaagga cgacctggag
    33601 acgatgaagc tgctcaaggt cgattgcctg gcgctgggca tgctgacctg catccgcaag
    33661 gcagtggccc tcatcgaacg ccatcgcggc tacacgatcg acctcgcgcg cctgccgccc
    33721 ggcgaccagc ccacctacga catgatccag gccgccgata ccgtgggcgt gttccagatc
    33781 gaatcgcgtg cgcagatgag catgctgccg cggctgaagc cgacctgctt ccacgacctg
    33841 gtggtggaag tggcgatcgt gcgccccggg ccgatccagg gcggcatggt gcatccctac
    33901 ctgcggcgcc ggcaggggct cgacccggtg gtgtatccct cgccgggcat cgaggac
//
LOCUS       DAOOCX010000010        33720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_972_length_33720_cov_6.848894, whole genome shotgun sequence.
ACCESSION   DAOOCX010000010 DAOOCX010000000
VERSION     DAOOCX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 33720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33720
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_972_length_33720_cov_6.848894"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="PK399_01780"
     CDS             <1..159
                     /locus_tag="PK399_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011269543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11890.1"
                     /translation="HAESNIDRVEYLERDTHTAAIRFLIEVSDRRHLAEVMRRIRRLG
                     VVHGVGRL"
     gene            199..591
                     /locus_tag="PK399_01785"
     CDS             199..591
                     /locus_tag="PK399_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPW11891.1"
                     /translation="MPRTAIHSDRAPAAIGPYSQATRAGNLVFFSGQIPLDPATGELV
                     AGDITAHARRAFDNLKAVCDAAGGTMDDIARVGLYLTDLSQFAAVNAVMGEYFTQPYP
                     ARSTIEVPALPKGAAFEVDAVMVLPLAD"
     gene            599..2698
                     /gene="recG"
                     /locus_tag="PK399_01790"
     CDS             599..2698
                     /gene="recG"
                     /locus_tag="PK399_01790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPW11892.1"
                     /translation="MPRLSRPAPALAASATPLSVLPGVGPAVAEKLAARGLSNLQDLW
                     LHLPRGYEDRTALTAIRDLRPGVAAQVEGRVTAVERGFRFRPMLRVALADDSRGTLVL
                     RFFHFRAQQVAQFAPGARIRAYGMPRAGQLGLEIVHPSYRVLDAGEHGLGEALDPVYP
                     EVEGIGPANLRKLIGLALQRLPDEDALELLPRDWLRELRLPTLREALLTLHAPPCEAD
                     VTALQTGLHPAQRRLALEELLAHQLSLRRQRLALQAEGTRALAGDERHVARLREALPF
                     ALTQAQARVYAQLREDLAQPRPMLRLVQGDVGSGKTVVAAMAALLAVADGRQAALMAP
                     TELLAEQHLANLRSWLEPLDIRVSWLAGKVTGKARAKALDEVASGAAQVVVGTHALMQ
                     EGVEFHDLALAIVDEQHRFGVHQRLALRDKGKGHVPHQLVMTATPIPRTLAMSAYADL
                     DVSAIDELPPGRTPITTIALSAERRAELVERIRQACAEGRQAYWVCTLIDDSDEIEAQ
                     AAQSTFEALQAALPDARIGLVHGRLKAAEKQSTMRAFKDGSIDLLVATTVIEVGVDVP
                     NASLMVIENAERLGLAQLHQLRGRVGRGSAASSCVLLYQAPLSAMARQRLDTLRQTND
                     GFAIAEKDLQLRGPGELLGTRQTGLAAFRIADLARDADLLPRVHALADRLLQDAPDAA
                     NRIIDRWVGGAARFAAA"
     gene            complement(2708..3589)
                     /locus_tag="PK399_01795"
     CDS             complement(2708..3589)
                     /locus_tag="PK399_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW11893.1"
                     /translation="MPAKPVVTPFFHAASNTWSYVVRDPASNAAAIIDPVLDFDAPSG
                     RTSTTSAQRIAEHVATNGLEIRWLLETHAHADHLSAAHWLKVTHYPGATLAIGVGIRA
                     VQKAFRPIFNLGEHFPVDGSQFDHLFADNEAFAIGDLQARVIAVPGHTSDSNAFLIGD
                     ALFTGDSLFMPDGGTARCDFPGGDAATLYRSIQRMYALPADTRVFVCHDYGPGGREVA
                     CETTIDAQRRGNIHVRDGVAEADFVAVRTARDATLAMPALLLPSVQVNIRAGALPEPE
                     ANGVRYLKLPIDQFGAS"
     gene            complement(3592..3942)
                     /locus_tag="PK399_01800"
     CDS             complement(3592..3942)
                     /locus_tag="PK399_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPW11894.1"
                     /translation="MAQLRRKSVPPTLDPEAMRAHAADAARLLRALANEKRLMLLCTL
                     VEGEKSVGELNSQVDLSQSALSQHLAVLRADGLVATRREAQTIYYSLAAGPAHRIIDT
                     LHGIYCGAAPLCEP"
     gene            complement(3947..4354)
                     /locus_tag="PK399_01805"
     CDS             complement(3947..4354)
                     /locus_tag="PK399_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008217344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPW11895.1"
                     /translation="MTQPITAADLVAEARAKIREIAPRDFHASRGTASLTDAVVIDVR
                     EPAEFETGHIPGSINIPRGVLEFQVDAHPAVANVSDPALSHKERPIVVVCRTGGRAAL
                     STVNLQRLGFGDVRSIAGGVVAWGEAGLPLVVR"
     gene            4448..5737
                     /locus_tag="PK399_01810"
     CDS             4448..5737
                     /locus_tag="PK399_01810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HPW11896.1"
                     /translation="MARIVVVGAGLGGMSAAYELRETLGKGHEVTLVGKGGMFGFNPS
                     NPWLAVGWRTPGEITLPVAEHVGKHGIAFNGDGVERIDAEGDAVITGTGERIGYDYLL
                     ICTGPRLAFEEVPGSGPDTGHTHSICTTAHAADAWQAYQDFLKDPGPIVIGAVQGASC
                     FGPAYEFAMIVDADLRKRKLRDKVPMTFVSSEPYIGHMGLGGVGDSKGLMESEMRQRH
                     IKWIVNSKVAEVREGEMTVVEHDAKGQPGESHALPFKFSMLLPAFKGVEAVAAVEGLC
                     NPRGFVIVDKHQRSPKYPKIFSAGVCVAIPPVEATPVPTGAPKTGFMIESMVTTIVRN
                     IQAELKGEAPDYEGTWNAVCLADMGDTGAAFVALPQIPPRNVTWTRIGKWVHVAKIGF
                     EKYFLYKMRNGTSEPIYEKYILGMLGIERLKDVKRSS"
     gene            5771..5983
                     /locus_tag="PK399_01815"
     CDS             5771..5983
                     /locus_tag="PK399_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPW11897.1"
                     /translation="MTLDRAVQAFAGIMVIVSLLLTKFVHPNFFWFTAFIGVNLFQSA
                     FTGFCPAGMVMRKLGIGRDAASTCSR"
     gene            5995..7095
                     /locus_tag="PK399_01820"
     CDS             5995..7095
                     /locus_tag="PK399_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW11898.1"
                     /translation="MRKPRDLLMPSLLALALSLAACGGTEQAAVPPLGAVATFEVAAA
                     DGATGRGWDGVVEAVRRSDLSAQTAGRVIAVEVDVNDRVAAGDVLLRISAVEQDAGAN
                     AARAQLRAAEASAAEAEQNYRRFAALANGQFVSKAQIDQARAARDSAAAARDAARAVV
                     AQAAQQSAYTVVRAPFAGVVASRDVEPGETVAPGQPLMRVYEPRELRIEVAVPQTRAE
                     AIRRDPRARVLLPGGREVTPAAVIVFPAADATSHSVNVRVRLPDLDPSPAPGTTAKVV
                     FDAEAGSAGDGGAATVRIPLSAVAQRGELSGAYVKQGERLLLRQLRLGARVGDSVEVI
                     SGLQAGDKVARDPVAAMQALAAQRKQAGATDE"
     gene            7088..>7506
                     /locus_tag="PK399_01825"
     CDS             7088..>7506
                     /locus_tag="PK399_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW11899.1"
                     /translation="MSESSTPARLGISGRLAAAFQKNPLTPILAIMGLLLGLLAVAIT
                     PREEEPQIDVTMANVIVPFDGASARDVEQLVATPLEQKLSEIEGVKHVYSISRPGMAV
                     MTVEYEVGVQRQPALVRLYNQVFSNQDWLPPGVGVGQP"
     assembly_gap    7507..7606
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7607..10088
                     /locus_tag="PK399_01830"
     CDS             <7607..10088
                     /locus_tag="PK399_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW11900.1"
                     /translation="GKPLLLADVASIEPRGDLATRYAWHGAPAGRDGPKAGIAPAVTI
                     AIAKKPGSNASDITSAIATRIGQLQGQLIPDGVHATVTRDYGATAADKAGKLMQKLVF
                     ATASVVLLVLFALGWREAIVVGSAVVLTLAVTLFASHVMGFTLNRVSLFALIFSIGIL
                     VDDAIVVVENIHRHLAIPGADGRKRSLFEVIPPAVDEVGGPTILATFTVIAALMPMAF
                     VSGLMGPYMRPIPINASVGMLLSLLIALVVTPWLSLKLLRRHGDVGDVAHAPEAQPQS
                     RLHRMFRKVMSPFLFGERAGRKRGLLFASMAGLVLLAASLAVFELVVLKMLPFDNKSE
                     VQVVVDMPEGSTLEQTNALLGELAVQLDTVPEVLDYQGYAGTAAPINFNGLVRQYFLR
                     SGSNVGDLQVNLVDKHHRDRKSHDIARAIRPALAAIGKRHGASVKVVEVPPGPPVLSP
                     LVAEVYGPDYAGQRRIARALASGPFATTEGIVDIDTSVESDAPRETLVLDRVRAARLG
                     VAQATVADAIAMAVSGADATYVQDGTSKYPRPVRVRLPAQEQASLDALLGVKVRGGSG
                     QLVPLSELVRVERGTWDGAIHRKDGLPVAYVMADEAGDLDSPLYGMFSIVGQLRDAGV
                     DGQSLAQTFISQPPGDSGYAVKWDGEWQITYETFRDMGIAYAAGMVLIYLLVVAQFRS
                     YLVPLVIMAPIPLTVVGVMPGHALLGAQFTATSMIGMIALAGIIVRNSILLVDFINQE
                     TARGVPLAEAVIDACAVRAKPIALTGAAAMLGAFFILDDPIFNGLAISLIFGILVSTV
                     LTLVVIPVLYYVLQAGRARRPAATMETT"
     gene            10088..10528
                     /gene="trxC"
                     /locus_tag="PK399_01835"
     CDS             10088..10528
                     /gene="trxC"
                     /locus_tag="PK399_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HPW11901.1"
                     /translation="MTATRLVACPHCNAINRVPMSRLAESPNCGKCAGALFDGHPLAL
                     DAVGFHAHAERADLPLLVDFWAPWCGPCLAMAPQFEAAAAQLEPAMRLAKVDTEAQPA
                     LGGRFGIRSIPTVVLFQQGRELARQAGAMGAADIVRWARHHLPR"
     gene            10537..11070
                     /locus_tag="PK399_01840"
     CDS             10537..11070
                     /locus_tag="PK399_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HPW11902.1"
                     /translation="MRQPFATIFVLASALGLLAGCQTHRAAPVPAVMPATSIELRTPR
                     PGLYTAGQPAAGDWSAIAARGVGTVIDLRAPGELKDRDEPAEVRAAGMRYLSIPVAGA
                     GGIDDANAQRLRTALEAANGPVLVHCASGNRVGGLLALMQARSGGMTTEQALEFGRKA
                     GMVGTEARVRELLDAGE"
     gene            11107..12045
                     /locus_tag="PK399_01845"
     CDS             11107..12045
                     /locus_tag="PK399_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HPW11903.1"
                     /translation="MTNDRIPLLIDTDPGVDDALALLMAFNDPRFDVVGLTIAAGNVG
                     LDHTVANALKLCEVCGVDVPVFPGADAPMVHPARDAAYVHGRDGFGDTGYVPASRQAE
                     AEHAALAILRLSHEHAGKLLLVALGPLTNVALALKLDPTLPSRIARCVIMGGSVTAHG
                     NITPAAEFNIAFDPEAAHIVCSAFPQFELADWEAVLAHGFQHDDADRWLAADSPRARF
                     YAGISAHTRAWSKAGRGARWHAADALAMALALRPEWAIETLERPLVVEMAGVHTRGGT
                     VVDWNRQTGQADNAAILMRYDQARFEGMIRAALAAG"
     gene            12137..12379
                     /locus_tag="PK399_01850"
     CDS             12137..12379
                     /locus_tag="PK399_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPW11904.1"
                     /translation="MKAGIHPEYRDVVFQDVTSDFKILTRSTLASKETVKWEDGNEYP
                     LIKVEVSSSSHPFYTGQNKLIDTSGRVDKFRKRYGK"
     gene            12577..13866
                     /locus_tag="PK399_01855"
     CDS             12577..13866
                     /locus_tag="PK399_01855"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPW11905.1"
                     /translation="MPELEQITLTAGDKSVAMPVLHPTLGQPCIDIAKLPKETGCFTY
                     DPGFGATASCKSAITYIDGDAGVLLYRGYPIEQLAEKSSFTEVAYLLMHGELPTQVEL
                     GKFEHEVTHHTMMHEAFRTFLYGFRHDAHPMAMLTGMLGSLASFYHNDLDLEDPEQRR
                     LAAIRLIAKVPTIAAACHRYSIGWPIRYPRNNLDYTARFLHMMMEVPSEPLELNPVAA
                     KAMDLLFILHADHEQNASTSTVRLVGSTGANPYVSVAAGVAALWGPAHGGANEAVLKM
                     LAEIGRPENVKSAIDKAKDKESGFRLMGFGHRVYKNYDPRAALVRKMTHEVLGALNVD
                     DPLLEVAVRLEEAALQDEYFVQRKLYPNVDFYSGIIYKALGIPTEMFTVMFAIARTAG
                     WVSHWLEQQNDPENRIGRPRQIYTGAPTRDYVGMGQR"
     gene            complement(13964..14593)
                     /locus_tag="PK399_01860"
     CDS             complement(13964..14593)
                     /locus_tag="PK399_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11906.1"
                     /translation="MHAQTRTRERRQRLAHEAARLMAEGGMRDYHQAKLKAAQRLGIH
                     DDASLPRNREIEDALREYQRLFAGSDQPAELRRRREAALRALDFFRDFEPRLVGAVLD
                     GTADANTAVSLHLHDDDADAVPRWLDEHGVPAESRSRHLRLDRDREGDFPVWLFGAEG
                     LSFDVTVLPADVLRQAPLSAIDEKPVRRASAVQLRALLADDEIAGYEGA"
     gene            complement(14697..17213)
                     /locus_tag="PK399_01865"
     CDS             complement(14697..17213)
                     /locus_tag="PK399_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HPW11907.1"
                     /translation="MPRLPRMLRWFLLGAVALCLIGLLAVGILVYVVSARLPDVQSLR
                     DTELQEPLYVYAADGRLMGLFGEMRRYPIDIARVPARVKQAFLAAEDANFYEHHGIDV
                     NGVARAVWLLAKTGGQERVAGGSTITQQVAKQFFLSSEYSFRRKFAEMLLAMKMEREL
                     GKDEILELYLNKSFFGNRAYGVAAAAEFYYGKTLDQLTLDEAATLAGIPKFPSSGNPL
                     GNPERARMRRDNYILPRMAELGYVSRAEADAARAVPMHATAHERQIEVYAPYVAEMVR
                     QQMIERFGGDVLSKGYHVTTTIDPVLQAAADRAVIDGLRVYDHRHGWRKPEQRVEIPA
                     GADAATIAGLLRGTPAQGGLLPAVVTGSEGGQLRVVLADGSELGLAGSAISWTGKSAG
                     GLAQRGDVVRVRRLPSDKADAAPQWVIDQLPRGQAALVSLDADNGAIRALVGGFSFAG
                     NKFNRATQARRQPGSSFKPFVYAAAFERGFNPASIVLDAPVVFKDRRGHMWRPQNDSG
                     NFAGPMRVREALVQSRNLVSVRLLDAIGVEYARRYISHFGFDEASLPPNLSMSLGTAS
                     LTPLSVARGYTAFANGGFLTTPWVIASVRDRDGKDIFTEKPPTACPGCAVTAIPGQPA
                     TAAPVPSKVDGFDLGPTVASTAPAAGATVAGAGKQPDAPKPREQKPLDPGMVVAPRAI
                     DPRVAWQLQSMMRDVVKRGTATDAKVLGREDVGGKTGSTNNHRDAWFSGFGGPVVTTV
                     WVGRDDFRSLGYREYGGKAALPIWISYMKTALKDQPERLPDPPDGMVKVGVGANGRLL
                     PGGGGITEWVKVEDLERMETDLGPAEDPNAQPAEEAFDIF"
     gene            17455..18513
                     /locus_tag="PK399_01870"
     CDS             17455..18513
                     /locus_tag="PK399_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPW11908.1"
                     /translation="MGLVTKTQSPLIGVDISSTAVKLLQLARTGDRYRVEHYAVEPLP
                     PNAVVEKNLVEVEAVGEAIRRAVARSGSRIKHAAAAVSGSSVITKVIPMPADLGEDDL
                     ESQIELEAVNYIPYPIEEVNLDFEVMGPMPGNSEMVQVLLAASRSENVEVRASALELG
                     GLTAKVIDVEAFAIENAYALMAGSLNVPRDGLAALVDVGATMTTLNVLRGGRSIYTRE
                     NVFGGKQLTDEVMRRYSLSYEEAGLAKRQGGLPESYEIEVLDPFKEAMVQQVSRLLQF
                     FYAGSEFNRVDSVVLAGGCASIPGIAAMVEEQLGIPTVVANPLAQMTLGPRVQAHALA
                     QDAPALMIACGLALRSFD"
     gene            18513..19145
                     /locus_tag="PK399_01875"
     CDS             18513..19145
                     /locus_tag="PK399_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005911890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HPW11909.1"
                     /translation="MARINLLPWRAERRKSRQKEFVGMLGLAALAGVLVAFLVYSYYG
                     GRISGQTERNDFLRGEITKVDAQIKEIEELDQKKSKLLARKEVIEQLQSNRSQMVHLF
                     DSLVRTIPDGVTLSAIKQEGDILTLSGRSQSNARVSTYMRTLESSGWMTKPDLNIIEA
                     KGANPGLPYEFTLKVTLANPNAPKEEEGEGGEATAPAAAPAAPAAGGAAP"
     gene            19142..19864
                     /gene="pilO"
                     /locus_tag="PK399_01880"
     CDS             19142..19864
                     /gene="pilO"
                     /locus_tag="PK399_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPW11910.1"
                     /translation="MSNNKKINLKELDINNIGGWPRQAQMGFCVIVAVIIILLGWWLF
                     VRGQQTELEKLEGQEKELRTEFENKQGRASNLEPLKQQLAQMEQQLQQMLRQLPSKTE
                     MPDLIVDISQTALATGIQNELFQPAGETKKEFYAEKPIALRMVGTYHQFGAFVSGVAS
                     LPRVVIMTMHDISLQPKDAKTNPNARIGPNSALELAGTVKTYRYLDEDETAADAAPAA
                     TDKAPAKGKAKAKAEAKEKGGA"
     gene            19956..20387
                     /locus_tag="PK399_01885"
                     /pseudo
     CDS             19956..20387
                     /locus_tag="PK399_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
     gene            20402..22399
                     /locus_tag="PK399_01890"
     CDS             20402..22399
                     /locus_tag="PK399_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ family protein"
                     /protein_id="HPW11911.1"
                     /translation="MTVTNAKQQRPLRRPLSLGTCAIGLAVGLYAAGASALPVTTLAA
                     MGQSATGAMADPAKRTPDAVTVSSIDFKRGDGGAGKLVLRFSGAGAAPDLRKTGNSVV
                     VDIGNAQLPAALQRPINVTDFATPVQRIDARATSNGAQLVLGTQGEFESMAYQSGNEY
                     IVEIVPRASMAGVAKPKAAAPAMGASAMGAATSASTVRYSGKPVTFNFQDVPVRTVLQ
                     LIAEESNLNIVAADTVQGNVTLRLINVPWDQALDIVLQAKSLDKRRSGNVVWVAPQKE
                     IAQFEQDKEDARISLEERAEKVTEYIPVNYASAEDIAKLLTEESKGNSGGASGGGQGG
                     GQNQQDRGFLSQRGSLSYDKRTNTLLVVDIPQRVANIRNLVQQLDKPVDQVVIEARIV
                     IANESVARELGARFGVSGNNGDSYYSGNLEANQNKINADKALDLQFAKDMSAWIAGGQ
                     VGARPVYSQSLPFRGLMSNLPAALQNPAGSLALSILNAGYQLDVELSAIQEQGRGEVI
                     SNPRIVTSNQKESVIRQGKEIGYLTITGSGLGATPTVAFKEALLELKVTPTITNDGRV
                     FLTLGVKKDELDGYVSMGTFGEVPQIAKREVNTAVLVDDGQTVVIGGVYEFSDTTELS
                     KVPFLGDLPVIGNLFRNKSRSKSKAELLVFVTPKVMRVTQR"
     gene            22536..23528
                     /locus_tag="PK399_01895"
     CDS             22536..23528
                     /locus_tag="PK399_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW11912.1"
                     /translation="MDKAIPDTATLHDAFRALREDLSREIVGQAGLIERLLIALLADG
                     HLLVEGAPGLAKTTAIRALASRLEADFARVQFTPDLLPADLTGTEVWRPQDGRFEFQP
                     GPIFHPILLADEINRAPAKVQSALLEAMGERQVTVGRQTYPLPPLFLVMATQNPIEQE
                     GTFPLPEAQLDRFLMHVRIGYPRVDAETEILRLARERARDVLQAASHAPARMPQAHVF
                     AARAAVLDLHFAPALERYLVEIVLASRDAGRYDAALARRIAWGASPRGSIALERCARA
                     RAWLEGRDFVTPEDVRAVAPDVLRHRVLPSYEATAEGWDGERLVSELLRLVPLP"
     gene            23425..24477
                     /locus_tag="PK399_01900"
     CDS             23425..24477
                     /locus_tag="PK399_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPW11913.1"
                     /translation="MCCAIACCPATKPPRKAGMASDWCPSCCAWCRCRKPATDRSEPG
                     ATVTDGIVPTLSELVGLRALVQGRRGARLGRHGVQGQALSNLRGRGMEYAESREYAQG
                     DDARHIDWRLTARSGRTHTKLFQAERERLTLVVADTAPSLYFGTRVRFKSVQAARAGA
                     LAAWLAARDGDRIAALRGSHREAPVPPASGPRGALRVLDALVRWYAQPPADDEGLAVA
                     LEHARRLLRPGSRLVLLADPSSIAAVPAERWPALAMHHELIVLLLVDPLETSPPAKRL
                     PFRGAPGDRIELGLDTAPVRQRWRQQFDIPLEATQAMLQRQGARVMTLSTIDASDAWL
                     SLLGRPNAVVATGAGR"
     gene            24474..24926
                     /locus_tag="PK399_01905"
     CDS             24474..24926
                     /locus_tag="PK399_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HPW11914.1"
                     /translation="MNMPLPLKDVHPGLAPSWWPPAPGWWMVAAVLALVGVAMWWWMR
                     RRRTRRASILRLFEGTVDAATSPAQQVAAMSDLLRRAARRKDPLADRLQGDDWLRFLD
                     QGMTPPCFEHGPGALLREGAFRRDVDEGAVRALRTVARQRYLTWMLGK"
     gene            24923..25927
                     /locus_tag="PK399_01910"
     CDS             24923..25927
                     /locus_tag="PK399_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPW11915.1"
                     /translation="MNLLADWLGIDGFAWPWLLLAIPLPLLARVLMPARANPQPALRV
                     PWGERLRRVASGGVLEVGSRGFPWMAMLAWCLLCVAAARPQQLGPPIAPPQVGRDMML
                     AVDLSASMGEEDMELGGRLVDRLTAAKAVLADFLDRRVGDRIGLVVFGDRAFALTPLT
                     LDRDSVRQQLDASVVGLAGRATALGDAIALSTKRLQQQRTEQRVLIVLTDGVNTAGVL
                     EPAKAAQIARDAGVRIHAIAFGGEGGGALSLFGLRLPAGGDEVDEAGLQKIAQLTGGR
                     FFRARDTEALAGIYAEIDALEPVQRQGQAVRPKIECYPWPLGLALAVGALAVLLRRRA
                     "
     gene            25924..27654
                     /locus_tag="PK399_01915"
     CDS             25924..27654
                     /locus_tag="PK399_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW11916.1"
                     /translation="MNLFPDVHFLRPWWLLALLVLPMLAWWWRRQAQQRNPWRDLVDA
                     HLLPHLLDRGAEQRAGVLPRWLALAATALAIIALAGPGIGKDEQPLWQAQEPLVIALD
                     LSSATLARDLPPTRLAQARAKLATLLRERAGGQVALVAFAGDAFTVAPLTEDAANVAL
                     FLDALDPGVMPDDGHRADRAIAWSQGLMRQAGFARGQILLLTDRADGTDIDAASKANA
                     AGYRVSVLGLGTEQGGVFDTPEGLGQARLDASGLKRLAASGGGGYQALSPGDADLRAL
                     GVLDPRQDAGTSVRGQKVSRWRDDGYWLLPVVLLLCLPLFRRGAAFALLLACGLWLPM
                     VPASAQSSSSGGSLWQRADQAEHARMQDGIAAYRQKDYPRAIERFSTVGGAEGQYNLG
                     NALAKAGRYDDAIAAYDRALELQPGMKDAAFNRNLVRVAKQLPKRPQDKGEDKDGKGG
                     KDGQPPQASKPGQQGQAGKPQAPPAQGKGGQAGKPPQAPPQKDPQSQAQADAAQRERM
                     QQALQKQQAKQPGQQPKPDQRGKPQETAEQRERRLANEAWLQRVPDDPGALLRARFQL
                     EQQRRRGATP"
     gene            27651..29288
                     /locus_tag="PK399_01920"
     CDS             27651..29288
                     /locus_tag="PK399_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPW11917.1"
                     /translation="MTRAIAWLLVCLLAFFASPSHAQTRAWLDRAQVSHGETATLNIQ
                     TDQSVASIDYTPLQRQFDIAGQTVRRSYQRSNGRSTATSLFAVGLRPRGPGVFTIPAL
                     RVGKTTTAPLRLTVVPPSVLPASGDADAFVETVVDAARPYVQQAVGVQVRLHYAVPLM
                     SGQLEQDPPTGASLRQVGEDLTYQRQIDGRRYNVVERRFLLIPERSGALVLPGARFNG
                     QTMGGFFDQVFDDGRKAVSAASPARRLDVLPIPTGAPQPWLPLHDLKLRYVQAPTQAR
                     AGEAASVEIEVIADGATATQLPPVALAETAGVQLFADPPQVDEQFVDGRPRSILRRRI
                     AIVPLQAGALSIAGPRIEWWDAAQGVARTAMLPSLQLQVAPGAAAATTSVDAAAPAGR
                     AGADAPSGDAAHAWSRRWPWLLLAAALLVVLAWWRWPRARVPAADACGDAPSPEAPAA
                     RIPSLPDALKRDDLGVIAQALGAAAGSHGEDLDAVRQRLDDAAQVEAVMQLQAARWGS
                     GDAAPTLAVLRAAFAKGPRWRKAAPASNSLLPPLYPE"
     gene            29386..30681
                     /locus_tag="PK399_01925"
     CDS             29386..30681
                     /locus_tag="PK399_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPW11918.1"
                     /translation="MSEAKAGSGKLQLHWKMLIGFLAGLALGLVAHYGAGSDAGWVQW
                     LTTYVTQPAGTLFLRLIFMLVIPLLFSALIIGVAEMGDVRSLGRVGWKTLGYTVIASG
                     IAVALGLLLVNWLQPGAGVDPAAAQAMLAQGADRAKDIVGSTASQPRGLDMLLAIVPD
                     NVVKAAAENTILAVMFFALMLGIGLVLTRTPATETLLRGIQGLFEVSMTLIGLVIRLA
                     PYAVFCFMFNLAALFGWDLLNSLGKYVGVVVLALALHMLVVYSLMLKFLGGWSPLKFF
                     KGSQEAMVMAFSTASSNATLPVSLRVAEEKLHLPKKISRFVLTVGATANQNGTALFEG
                     VTVLFLAQFFGVDLSLTQQVTVMFVCILGGIGTAGVPAGSLPVVALICGMVGVPPEGI
                     GLILGVDRFLDMCRTTLNVTGDLMLAAVVSKGEEDPETA"
     gene            30775..32760
                     /gene="tkt"
                     /locus_tag="PK399_01930"
     CDS             30775..32760
                     /gene="tkt"
                     /locus_tag="PK399_01930"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPW11919.1"
                     /translation="MTTPSRRDLANAIRFLAIDAVQAANSGHPGMPMGMADIAEVLWN
                     DVLKHNPANPRWADRDRFILSNGHGSMLQYALLHLSGYDLSIDDLRQFRQLDSKTPGH
                     PENFMTCGIETTTGPLGQGLANAVGFALAEKLLAQRFNRDGHAIVDHRTWVFMGDGCL
                     MEGISHEAASLAGTWGLGKLVAFWDDNHISIDGNTDGWFTDDTPKRFEAYGWNVVRGV
                     DGHDPVEIRTAIETARKSSDKPTLICCRTTIGFGSPNKAGKESSHGSPLGKDEVVATR
                     EALGWEHGAFDIPQALYDGWRARDLGAVEADWDARFAAYAAAFPSEAAEFQRRLAGDL
                     PDGFVDAALAFAIKTQQDGPSIASRKASQNCIEAFAPLLPELVGGSADLAHSNLTLWK
                     GSKSVATDDANANYVYYGVREFGMTAIANGLGLHGGFIPYDATFLVFSDYARNAVRMA
                     ALMGVRALHVYTHDSIGLGEDGPTHQPIEHLASLRYIPDNDVWRPCDAVESAMAWKAA
                     IQRDDGPSCLIFSRQNLPHQARDEGQLLDIARGGYVLKDSVGTPEVILIATGSEVGLA
                     MQAAEQLGDGVRVVSMPCTDVFDRQDAAYRESVLPKAVRKRVAIEAGVSDFWRKYVGL
                     DGAVIGIDTFGASAPIEALMPHFGFTVEHLIQVIREL"
     gene            32819..33181
                     /locus_tag="PK399_01935"
     CDS             32819..33181
                     /locus_tag="PK399_01935"
                     /inference="COORDINATES: protein motif:HMM:NF022610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HPW11920.1"
                     /translation="MARLALAAILLLAGVPTLGRWMDAGREAPALAATAMCTSEGMAW
                     SAPSSLFGSDPIPAGTQTGEDCAYCPLLASAVPLVLATLLLSPPPAPPPAVDIPVAPA
                     SDAGHAHGLGARGPPLFS"
     gene            33242..>33720
                     /locus_tag="PK399_01940"
     CDS             33242..>33720
                     /locus_tag="PK399_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPW11921.1"
                     /translation="MSRFPLATALPSRLALACAISLAAFPAAASDDEAKTLDRVIVTA
                     TPIQPLTFETDPKLPRQPVPASDGADYLKTIPGFTALRNGGTNGDPVLRGMFGSRLNL
                     LSNDGSMPGACPARMDNPLSYVAPETYDRLTVVKGPQTVLWGPGASAGTVRFQRDTPR
                     "
BASE COUNT         5268 a        11297 c        11724 g         5331 t
ORIGIN      
        1 catgccgaat ccaacatcga ccgcgtcgaa tacctcgagc gcgacaccca cactgcggcg
       61 atccgcttcc tgatcgaggt cagcgaccgc aggcacctgg ccgaagtgat gcgccggatc
      121 cgccgcctgg gcgtggtcca cggggtcggc cggctgtagc gtcgttacac tgtcggcttc
      181 cccgacggag tgtcccgcat gccccgcacc gccatccaca gcgaccgcgc gccggccgcg
      241 atcgggccgt attcacaggc gacccgcgcc ggcaacctgg tcttcttcag cgggcagatc
      301 ccgctggacc cggccaccgg cgaactcgtc gccggtgaca tcaccgcgca cgcgcggcgc
      361 gccttcgaca acctcaaggc cgtgtgcgat gccgcgggtg gcacgatgga cgacatcgcc
      421 cgcgtcggcc tgtacctgac cgacctctcg cagttcgccg ctgtgaatgc agtgatgggc
      481 gagtacttca cccagccgta tccggcgcgc tcgaccatcg aagtcccggc cttgccgaaa
      541 ggcgcggcgt tcgaggtcga tgcggtgatg gtcctgccgc tggcggactg agcccggcgt
      601 gccgcgcctg tcgcgacccg cgcccgccct ggcggccagt gcgacgccgc tgtcggtgct
      661 gccgggcgtg ggccccgcgg ttgcggagaa gctggccgcg cgcgggcttt ccaacctgca
      721 ggacctgtgg ctgcacctgc cgcgcggcta cgaggatcgc accgcgctga ccgcgatccg
      781 cgacctgcgt ccgggggtgg ccgcgcaggt cgagggcagg gtgacggcgg tcgaacgcgg
      841 gttccgtttc cggccgatgc tgcgggttgc gctggcggac gactcgcgcg gcaccctggt
      901 gctgcggttc ttccatttcc gtgcccagca ggtcgcgcaa ttcgcgccgg gcgcgcgcat
      961 ccgcgcctac ggcatgccgc gcgcgggcca gctcggcctg gagatcgtgc atccgagcta
     1021 tcgcgtgctc gatgccggcg agcacggcct gggcgaggcg ctggatccgg tgtaccccga
     1081 ggtcgagggc atcgggccgg ccaacctgcg caagctgatc ggcctcgcgc tgcagcgttt
     1141 gcccgacgag gacgcgctgg aattgctgcc gcgcgactgg ctgcgcgagc tgcgcctgcc
     1201 caccttgcgc gaggcactgc tgaccctgca cgcaccgccg tgcgaggccg acgtcaccgc
     1261 cttgcagacg ggtttgcatc cggcgcaacg gcgactggcg ctggaggaat tgctggcgca
     1321 tcagctgagc ctgcgtcggc aacgtctcgc actgcaagcc gagggcacgc gcgcgctggc
     1381 cggcgatgaa cgccatgtcg cacgcctgcg cgaggcactg ccgttcgcgc tcacccaagc
     1441 gcaggcacgg gtgtacgcgc aattgcgcga ggacctggcg cagccgcggc cgatgctgcg
     1501 cctggtgcag ggcgacgtcg gcagcggcaa gaccgtggtt gccgccatgg cagccttgct
     1561 cgcggtcgcg gatggtcgcc aggccgcatt gatggcgccg accgaactgt tggcggagca
     1621 acacctggcc aacctgcgca gctggctgga gccgctcgac atccgggtca gctggctggc
     1681 cggcaaggtc accggcaagg cgcgggcgaa ggcattggac gaggtcgcgt cgggcgcggc
     1741 gcaggtcgtc gtcggcacgc acgcattgat gcaggagggc gtcgagttcc acgatcttgc
     1801 cctggccatc gtcgacgagc agcaccgctt cggcgtgcat caacgcctgg ccttgcgcga
     1861 caagggcaag ggccatgtgc cgcaccagct ggtgatgacc gccaccccga tcccgcgcac
     1921 gctggcgatg agcgcctatg ccgacctcga cgtctccgcc atcgatgaac tgccaccggg
     1981 acgtaccccg atcaccacca tcgcgttgtc ggcggaacgc cgcgcggaac tggtcgaacg
     2041 gatccgccag gcctgcgcgg aggggcgcca ggcgtactgg gtatgcaccc tgatcgacga
     2101 ctccgacgag atcgaggcgc aggccgcgca atcgaccttc gaggccttgc aggcggcctt
     2161 gccggatgcc cgcatcggcc tggtccacgg ccggctgaag gccgccgaga agcaatcgac
     2221 gatgcgcgcg ttcaaggacg gcagcatcga cctgctggtg gcgaccacgg tgatcgaagt
     2281 cggcgtggac gtgccgaacg cgtcgttgat ggtgatcgag aacgcggagc ggctgggctt
     2341 ggcgcagttg caccagttgc gcgggcgggt ggggcgcggc agcgcggcat cgagttgcgt
     2401 gctgctgtac caggcgccgc tgtcggcgat ggcgcggcag cggctggaca ccttgcggca
     2461 gaccaacgat ggcttcgcca tcgccgagaa agacctgcaa ctgcgcggtc ccggcgaact
     2521 gctggggacg cgccagaccg gcctggcggc cttccgcatc gccgacctcg cccgtgatgc
     2581 ggatttgctg ccgcgggtgc atgcgctggc cgaccggttg ctgcaggacg cgcccgatgc
     2641 ggcgaaccgc atcatcgacc gctgggtcgg cggcgcggcg cgtttcgccg ccgcctgacc
     2701 cgcgcggcta cgacgcgccg aactggtcga tcggcagctt gaggtagcgc acgccgttgg
     2761 cttccggctc cggcaacgca ccggcgcgga tgttcacctg caccgatggc agcaacagcg
     2821 ccggcatcgc cagtgtcgcg tcgcgcgcgg tgcgcaccgc cacgaagtcg gcttcggcga
     2881 caccatcgcg cacgtggatg ttgccgcgcc gctgggcgtc gatcgtggtc tcgcaggcca
     2941 cctcgcggcc gcccgggcca tagtcgtggc agacgaacac gcgggtgtct gcgggcaacg
     3001 cgtacatgcg ctggatcgag cggtacaggg tggcggcatc gccgcccggg aaatcgcacc
     3061 gtgcggtgcc gccatcgggc atgaacagcg agtcgccggt gaacagcgca tcgccgatca
     3121 ggaacgcatt gctgtcgctg gtatggcccg gcaccgcgat gacccgggcc tgcaagtcgc
     3181 cgattgcgaa tgcctcgttg tcggcgaaca gatggtcgaa ctgcgagccg tcgacaggga
     3241 aatgctcgcc caggttgaag atcggccgga atgccttctg cacggcgcgg atgcccacgc
     3301 cgatggcgag cgtggcgccg ggatagtgcg tgaccttcag ccagtgcgcg gcgctcaggt
     3361 ggtcggcatg cgcatgcgtc tccagcagcc agcgaatctc caggccgttc gtggcgacgt
     3421 gctcggcgat ccgctgcgcg gatgtggtcg aagtccgtcc cgatggcgca tcgaagtcga
     3481 gcaccgggtc gatgatcgcc gccgcgttcg aggcgggatc gcggaccacg tagctccagg
     3541 tattgctggc tgcgtggaaa aacggcgtca cgaccggttt ggcgggcatg ttcatggctc
     3601 gcagaggggc gcggcgccgc aatagatccc gtgcagggtg tcgatgatgc ggtgcgccgg
     3661 gccggcggcc aacgagtagt agatcgtctg cgcttcgcga cgcgttgcca ccaatccgtc
     3721 cgcacgcaag accgccaggt gctgcgagag cgcggactgg ctcaggtcga cctgcgaatt
     3781 gagttcgccg acggatttct cgccctcgac cagcgtgcac aacagcatca agcgcttctc
     3841 gttggccagc gcacgcagca ggcgcgcggc atcggcggca tgcgcgcgca tggcctcggg
     3901 atccagcgtg ggcggaacgg atttgcggcg gagctgggcc atggtcttac cgtaccacca
     3961 gcggcaggcc agcttcgccc cacgccacca cgccgccggc gatcgagcga acgtcgccga
     4021 agccgagccg ctgcaggttg accgtggaca gcgcggcgcg cccgccggtg cggcagacca
     4081 ccacgatcgg gcgttccttg tgcgacagcg cggggtcgct gacgttggcc acggccgggt
     4141 gggcatcgac ctggaactca agcacgccgc gcgggatgtt gattgaaccg ggaatgtgcc
     4201 cggtctcgaa ttccgccggt tcgcgaacgt cgatcacgac ggcgtcggta agcgacgcgg
     4261 tgccgcgcga tgcatggaag tcgcggggcg cgatctcgcg gatcttcgcg cgggcctcgg
     4321 cgaccaggtc ggcggcggtg atgggctggg tcattgcagg ctccttgaaa tatcagttga
     4381 cactaatata agacagaact aatattaaat ccagagcctg catgggcgtc ctgggaggga
     4441 gcaagcaatg gctaggatcg tggtggtggg agcggggttg ggtggcatga gtgcggccta
     4501 cgagttgcgc gagacgctcg gcaaggggca tgaggtcacg ctggtgggca agggtgggat
     4561 gttcgggttc aacccgtcga acccctggct tgcggtcggt tggcgcaccc cgggggaaat
     4621 caccctgccg gtggccgagc atgtcggcaa gcacgggatt gcgttcaatg gcgacggcgt
     4681 ggagcgcatc gacgccgaag gcgacgccgt gatcaccggc accggcgagc gcatcggcta
     4741 cgactacctg ctgatctgca ccggcccgcg cctggccttc gaggaagtgc cgggcagcgg
     4801 tccggacacc ggccataccc actcgatctg cacgaccgcg catgccgcgg atgcgtggca
     4861 ggcctaccag gacttcctga aggatccggg cccgatcgtg atcggcgcgg tgcagggcgc
     4921 cagctgcttc ggtcccgcct acgaattcgc gatgatcgtc gatgccgacc tgcgcaagcg
     4981 caagctgcgc gacaaggtgc cgatgacctt cgtcagcagc gagccctaca tcggccacat
     5041 gggcttgggc ggtgtcggcg attccaaggg cctgatggaa tcggagatgc gccagcggca
     5101 catcaagtgg atcgtcaaca gcaaggtcgc cgaggtgcgc gagggcgaga tgaccgtggt
     5161 cgagcacgac gccaagggcc agcccggcga atcgcacgcg ttgccgttca agttctcgat
     5221 gctgctgccg gcgttcaagg gcgtggaggc ggtggccgcg gtcgaaggcc tgtgcaaccc
     5281 gcgcggcttc gtgatcgtcg acaagcacca gcgcagcccg aagtacccga agatcttctc
     5341 ggccggcgtg tgcgtggcga tcccgccggt ggaagccacc ccggtgccga ccggcgcgcc
     5401 gaagaccggc ttcatgatcg agtcgatggt gaccaccatc gtccgcaaca tccaggccga
     5461 gctgaagggc gaggcgcccg actacgaagg cacctggaac gcggtctgcc tggccgacat
     5521 gggcgatacc ggcgcggcct tcgtggcgct gccgcagatc ccgccgcgca acgtgacctg
     5581 gaccaggatc ggcaagtggg tgcacgtggc gaagatcggc ttcgagaagt acttcctcta
     5641 caagatgcgc aacggcacgt ccgaaccgat ctacgagaaa tacatcctcg gcatgctcgg
     5701 catcgagcgc ctgaaggacg tcaagcgctc ttcctgagcc catttcccca tctccaagaa
     5761 ggattccccg atgactctcg accgcgccgt acaagccttc gccggcatca tggtgatcgt
     5821 cagcctgctg ctgaccaagt tcgtccaccc caacttcttc tggttcaccg ccttcatcgg
     5881 cgtcaacctg ttccagtccg cattcaccgg gttctgcccg gcggggatgg tcatgcgcaa
     5941 gctgggcatc ggccgcgacg cagcgtctac ctgcagccgt tgatggatcc gctcatgcgc
     6001 aagccccgcg acctgctgat gccttcgctg ctcgcgctgg cgctgtcgct ggccgcctgt
     6061 ggcggcaccg agcaagcggc cgttccgccg ttgggcgccg tcgcgacctt cgaagtcgcg
     6121 gccgccgacg gcgcgaccgg ccgcggctgg gacggcgtgg tggaagcggt ccgccgctcg
     6181 gacctgagcg cgcagacggc cggccgcgtc atcgcggtcg aggtcgacgt caacgaccgc
     6241 gtcgccgccg gcgacgtgct cctgcgcatc agtgcggtcg aacaggacgc cggtgccaac
     6301 gccgcgcgcg cccagttgcg cgcggccgaa gcgtccgccg ccgaggccga gcagaactac
     6361 cgccgcttcg ccgcgctggc gaatggtcag ttcgtatcga aggcacagat cgaccaggcg
     6421 cgcgccgccc gtgattccgc ggcagccgcg cgcgatgccg cgcgcgccgt ggtggcgcaa
     6481 gccgcgcagc agtcggctta tacagtggtg cgcgcgccgt tcgccggcgt ggtcgccagc
     6541 cgtgatgtcg agcccggcga aaccgtagcc cccggccagc cgttgatgcg ggtgtacgaa
     6601 ccgcgcgaac tgcgcatcga ggtcgccgtg ccgcagaccc gcgccgaggc gatccgccgc
     6661 gatccgcgtg cgcgggtgct gctgcccggc ggacgcgagg taacgcctgc ggcggtgatc
     6721 gtgttcccgg cggcggatgc caccagccat agcgtcaacg tgcgcgtgcg cttgcccgac
     6781 ctcgatccgt cgccggcacc cggcaccacc gccaaggtcg tgttcgatgc ggaagcaggg
     6841 tccgccggcg atggcggcgc ggcaaccgtg cgcatcccgc tgtccgcggt cgcccagcgc
     6901 ggcgagctca gcggcgccta cgtgaaacag ggcgagcgcc tgctgcttcg ccagctgagg
     6961 ctgggcgctc gggtgggcga cagcgtcgag gtgatcagcg gcctgcaggc cggcgacaag
     7021 gtcgcacgcg atccggtcgc ggcgatgcag gcgttggccg cgcagcgcaa gcaggcgggc
     7081 gcaaccgatg agtgagtcgt ccactcccgc gcggctcggc atctccgggc gcctggcagc
     7141 cgcgttccag aagaacccgc tgaccccgat cctggcgatc atgggcctgc tgctgggcct
     7201 gctcgcggtg gcgatcaccc cgcgcgagga agaaccgcag atcgacgtga cgatggccaa
     7261 cgtcatcgtc ccgttcgatg gcgccagcgc gcgcgatgtc gaacagctgg tggcaacgcc
     7321 gctggagcag aagctgtcgg agatcgaggg cgtcaagcac gtctattcga tcagccgccc
     7381 cggcatggcg gtgatgaccg tcgaatacga agtcggcgtg cagcgccagc cggcgctggt
     7441 gcgcctgtac aaccaggtgt tctccaacca ggactggctg ccgccgggcg tcggcgtcgg
     7501 ccagccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggc aagccgttgc
     7621 tgctggccga cgtcgccagc atcgagccgc gcggcgacct ggccacgcgc tacgcctggc
     7681 atggcgcgcc cgcagggcgt gatggcccga aggcgggcat tgcgccggcg gtcacgatcg
     7741 ccatcgccaa gaagccgggc agcaatgcct cggacatcac atcggccatc gccacccgca
     7801 tcgggcaatt gcagggccag ctgatccccg atggcgtgca tgccacggtc acccgcgact
     7861 acggcgcgac cgccgcggac aaggccggca agctgatgca gaaactggtg ttcgcgaccg
     7921 cctcggtggt cctgctggtg ctgttcgcat tgggttggcg tgaggccatc gtggtcggca
     7981 gcgccgtggt gctgaccctc gccgtgaccc tgttcgcctc gcatgtgatg ggcttcaccc
     8041 tcaatcgcgt ctcgctgttc gcgctgattt tctcgatcgg catcctggtc gacgatgcga
     8101 tcgtggtggt cgagaacatc catcgccacc tcgccattcc cggcgccgat ggccgcaagc
     8161 gcagtctgtt cgaggtgatc ccgcctgcgg tggacgaagt gggcgggccg accatcctcg
     8221 ccacgttcac cgtgatcgcc gcgctgatgc cgatggcctt cgtgtccggg ctgatggggc
     8281 cgtacatgcg gccgatcccg atcaatgcct cggtcggcat gctgctgtcg ctgctgatcg
     8341 cgctggtcgt gacgccttgg ctgtcgctga agctgctgcg ccggcacggc gacgtcggcg
     8401 acgtcgccca tgcgcccgag gcgcaaccgc aaagccggtt gcatcgcatg ttccgcaagg
     8461 tgatgtcacc gttcctgttc ggcgaacgtg ccggtcgcaa gcgcggcttg ctgttcgcgt
     8521 cgatggccgg gctggtgctg ctggcggcat cgctggcggt gttcgagttg gtggtgctga
     8581 agatgctgcc gttcgacaac aagtcggagg tgcaggtcgt ggtcgacatg cccgagggca
     8641 gcacgctgga gcagaccaat gcgttgctgg gcgagctggc cgtgcagctc gacacggtgc
     8701 ccgaagtgct cgactaccag ggctatgccg gcaccgccgc gccgatcaat ttcaacggtt
     8761 tggtgcgcca gtatttcctg cgcagcggca gcaatgtcgg cgacctgcag gtcaacctgg
     8821 tcgacaagca ccaccgcgac cgcaagagcc acgacatcgc ccgcgccatc cgccccgcgc
     8881 ttgccgccat tggcaaacgc catggcgcct cggtgaaggt ggtggaagtc ccgcccggtc
     8941 cgcccgtgct gtcgccgttg gtggccgagg tctatggccc cgactacgcc ggccagcgcc
     9001 gcatcgcgcg cgcactggca agcggcccgt tcgcgaccac cgagggcatc gtcgacatcg
     9061 ataccagcgt ggaaagcgat gcgccgcgcg agacccttgt gctcgaccgc gtgcgcgcgg
     9121 cgcgcctggg cgtcgcccag gccaccgtcg ccgatgcgat cgcgatggcg gtgtcgggtg
     9181 ccgatgccac ctacgtccag gatggcacct cgaagtatcc gcgccccgtg cgcgtgcgcc
     9241 tgcccgcgca ggagcaggcg tcgctggatg cgttgctcgg cgtgaaggtg cgcggcggct
     9301 cgggccagct ggtgccgctg tcggagctgg tgcgcgtcga acgcgggacc tgggatggcg
     9361 cgatccaccg caaggacggg ctgccggtgg cctatgtgat ggcggacgag gcgggcgacc
     9421 tcgacagccc gttgtacggg atgttttcca tcgtcggcca gttgcgcgac gccggcgtgg
     9481 atggccagtc gctcgcgcag acgttcatct cgcagccgcc tggcgacagc gggtatgcgg
     9541 tcaagtggga cggcgagtgg cagatcacct acgaaacctt ccgcgacatg ggcatcgcct
     9601 acgcggccgg gatggtgctg atctacctgc tggtggtcgc ccagttccgc agctacctgg
     9661 tgccgctggt gatcatggcg ccgatcccgc tgaccgtggt cggggtgatg cccgggcatg
     9721 cgctgctggg cgcgcagttc accgccacca gcatgatcgg catgatcgcg ctggccggga
     9781 tcatcgtccg caactcgatc ctgctggtcg acttcatcaa ccaggaaacc gcgcgcgggg
     9841 tgccgctggc cgaggccgtg atcgatgcct gcgcggtgcg cgccaagccc atcgcgctga
     9901 ccggcgcggc ggcgatgctg ggcgcgttct tcatcctcga cgacccgatc ttcaacggcc
     9961 tggccatctc gttgatcttc ggcatcctgg tcagcacggt gttgacgctg gtggtgatcc
    10021 cggtgcttta ttacgtgctg caagccggca gggcacgccg tccagcggcg acgatggaga
    10081 ccacgtgatg accgcaacac ggttggtggc ctgtccgcat tgcaatgcga tcaatcgggt
    10141 gccgatgtct cggttggcgg aatccccgaa ctgtggcaaa tgcgccgggg cgctgttcga
    10201 cgggcatccg ctggccctgg acgccgtcgg tttccatgcc catgccgagc gcgccgacct
    10261 gccgttgctg gtggatttct gggcgccatg gtgcgggcca tgcctggcca tggccccaca
    10321 gttcgaggcc gccgccgcgc agctggagcc ggcaatgcgg ctggccaagg tcgataccga
    10381 ggcgcagccc gcgctgggcg ggcgcttcgg gatccgcagc attcccaccg tcgtgctgtt
    10441 ccagcagggc cgtgagcttg cccgccaggc cggcgcgatg ggcgcggccg acatcgtgcg
    10501 ctgggcgcgc caccaccttc cacgttgagg actcccatgc gtcaaccatt cgcgaccatc
    10561 ttcgtcctgg cctccgccct cggcctgctt gcgggttgcc agacccatcg cgcggcccct
    10621 gtgccggctg tgatgcctgc gaccagcatc gagttgcgga cgccccgtcc cggcctctac
    10681 accgcaggcc agcccgcagc cggtgactgg tcggcgatcg cggcgcgcgg cgtcggcacg
    10741 gtgatcgacc tgcgcgcccc gggcgagctc aaggatcgcg atgaacccgc cgaagtccgc
    10801 gcggccggca tgcgttacct ctcgatcccg gtcgccggcg cgggcggcat cgacgatgcc
    10861 aacgcccagc gcttgcgcac ggcactggag gcggcgaatg gcccggtgct ggtgcattgc
    10921 gccagcggca accgcgtcgg cggcctgctg gcgctgatgc aggcgcgatc cggcgggatg
    10981 acgaccgaac aggcgctcga gttcggccgc aaggccggca tggtcggcac cgaggcgcgg
    11041 gtcagggaat tgctcgacgc cggggagtga ggtcggatcg ccgacgcatc gggccataat
    11101 ggctggatga caaacgaccg tatcccgctc ctgatcgata ccgaccccgg cgtggacgac
    11161 gcgctggccc tgttgatggc cttcaacgac ccgcgctttg atgtggtcgg cctgaccatc
    11221 gccgccggca atgtgggcct ggaccacacc gtcgccaatg ccctgaaact gtgcgaggtc
    11281 tgcggcgtcg acgttcccgt gttcccgggc gccgatgccc cgatggtgca tcccgcgcgc
    11341 gatgccgcct acgtgcacgg ccgcgacggc ttcggcgaca ccggctacgt gccggcatcg
    11401 cgccaggccg aggccgaaca cgcggcgctg gcgatcctgc gcctctccca cgaacacgcc
    11461 ggcaagctgc tgctggttgc gctgggcccg ctgaccaatg tcgcgctggc gctgaagctg
    11521 gacccgaccc tgccgagccg gatcgcgcgc tgcgtgatca tgggtggctc ggtgaccgcc
    11581 cacggtaaca tcaccccggc cgccgaattc aatatcgcct tcgaccccga ggccgcgcac
    11641 atcgtctgca gcgcgttccc gcagttcgag ctggccgact gggaagcggt gctggcgcac
    11701 ggcttccagc acgatgacgc ggaccgctgg ctggccgcgg attcgccgcg ggcacgcttc
    11761 tatgccggga tctccgcgca tacccgggcc tggtcgaagg ccgggcgtgg ggcgcgctgg
    11821 cacgccgccg atgcgttggc gatggccctg gcgctccggc ccgagtgggc catcgagacg
    11881 ctggagcggc cgctggtggt ggagatggcc ggcgtgcata cccgtggtgg cacggtggtg
    11941 gactggaatc gccagacggg ccaggcggac aacgccgcca tcctgatgcg ttacgaccaa
    12001 gcgcgcttcg aaggcatgat ccgggccgcc cttgcggcag gctgatccgg cctgtcgtgc
    12061 tgatcggctt gcgccacccc gaaatccgtc gctataatgc gcggctctgt cccgctccaa
    12121 tacacaggtc caagccatga aagccggcat ccatcccgaa taccgtgacg tcgtcttcca
    12181 ggacgtgacc tccgatttca agatcctgac ccgctcgacg ctggcctcca aggagaccgt
    12241 gaagtgggaa gacggcaacg aatacccgct gatcaaggtg gaagtgtcgt cgtcgtcgca
    12301 cccgttctac accggccaga acaagctgat cgacaccagc ggccgcgtcg acaagttccg
    12361 caagcgctac ggcaagtaag cccagtcgcc atgcgttgac gaacggccac cctcgcggtg
    12421 gccgtttcgc tgtgggcaac cggaagcccc gcccggccgg cgtccgggac tttcgtcggg
    12481 ggtcgggtcc ggatggcctg tgcgataatt ctggttcccc atgcgcaggc gcatggcctg
    12541 cgggccggcg ccgcgagacc ccactggagt tgcgtcgtgc ctgaacttga gcagatcacc
    12601 ctgaccgcgg gcgacaaatc cgtcgccatg cccgtgctgc acccgaccct gggccagccc
    12661 tgcatcgaca tcgccaagct gccgaaggaa accggctgct tcacctacga tccgggcttc
    12721 ggtgccaccg ccagctgcaa gtcggcgatc acctacatcg atggcgatgc cggcgtgctg
    12781 ctgtatcgcg gctacccgat cgagcagctg gccgagaagt cgagcttcac cgaggtcgcc
    12841 tacctgctga tgcacgggga actgccgacc caggtcgagc tcggcaagtt cgagcacgag
    12901 gtcacgcacc acacgatgat gcatgaggcg ttccgcacgt tcctgtacgg gttccgccac
    12961 gacgcccacc cgatggcgat gctcaccggc atgctgggct cgctggccag cttctatcac
    13021 aacgacctcg atctcgagga tccggaacag cgccgcctgg ccgcgatccg gttgatcgcc
    13081 aaggtgccga cgatcgccgc cgcctgccat cgctattcga tcggctggcc gatccgctac
    13141 ccgcgcaaca acctcgacta caccgcgcgc ttcctgcaca tgatgatgga agtgcccagc
    13201 gagccgctgg agctcaatcc ggtcgccgcc aaggcgatgg acctgctctt catcctgcat
    13261 gccgaccacg agcagaacgc gtcgacctcg accgtgcgcc tggtcggttc caccggtgcg
    13321 aatccctacg tcagcgtcgc cgccggcgtg gcggcgttgt gggggccggc gcatggcggt
    13381 gccaacgagg cggtgctgaa gatgctggcg gagatcggtc gcccggagaa cgtgaagtcc
    13441 gcgatcgaca aggccaagga caaggaatcc ggtttccgcc tgatgggctt cggccaccgt
    13501 gtgtacaaga actacgaccc gcgcgcggcg ctcgtgcgca agatgaccca cgaagtgctg
    13561 ggcgccctca acgtcgacga cccgttgctg gaagtggcgg tgcggctgga ggaagcggcc
    13621 ctgcaggacg agtacttcgt ccagcgcaag ctctacccga acgtcgattt ctacagcggc
    13681 atcatctaca aggcgctggg catcccgacc gagatgttca ccgtgatgtt cgcgattgcc
    13741 cgcaccgccg gctgggtcag ccactggctg gagcagcaga acgatccgga gaaccgcatc
    13801 ggtcgcccgc gccagatcta taccggtgcg ccgacccgcg actacgtggg catgggccag
    13861 cgctgagtcg cggctcgacc ctgcatcgca gaacgccccg ccatgcgggg cgttttcgtt
    13921 cctggaaggc gcagggaggg gggcgggaac gccgcagggc gcttcacgcg ccttcgtaac
    13981 cggcgatctc gtcgtcggcc agcagtgccc gcagctgcac cgcggacgcg cgccggaccg
    14041 gcttctcatc gatcgcggac agtggtgcct ggcgcaggac gtcggcgggc agcacggtca
    14101 cgtcgaagct caggccttcc gcgccgaaca gccagaccgg gaagtcgccc tcgcggtcgc
    14161 ggtccaggcg caggtggcgg ctgcgcgatt ccgccggcac gccgtgttcg tccagccagc
    14221 gcgggaccgc atcggcatcg tcgtcgtgca ggtgcaggga cacggcggtg ttggcatctg
    14281 cggtgccgtc cagcacggca ccgaccaggc gcggttcgaa gtcgcggaag aagtccaggg
    14341 cgcgcagggc ggcctcgcgg cggcggcgca gttccgcggg ctggtcgctg ccggcgaaca
    14401 ggcgctggta ttcgcgcagg gcatcctcga tctcgcggtt gcgtggcagc gaggcgtcgt
    14461 catggatgcc gagccgctgc gcggccttga gcttggcctg gtggtagtcg cgcatcccgc
    14521 cctcggccat cagccgggcg gcttcatggg caagccgctg gcggcgctcg cgggtgcggg
    14581 tctgcgcatg catgtgcgcg tgggaaccgg cgtgctggcg gctgttcggc atcgcgcaat
    14641 cccgggatgg cctgccggga ctgtaaccga gcgggtgtgg ccccgccatc gcgtcgtcag
    14701 aagatgtcga aggcttcttc cgcgggttgc gcgttcggat cctccgcggg ccccaggtcg
    14761 gtttccatgc gctccaggtc ctcgaccttc acccactcgg tgatcccgcc accgccgggg
    14821 agcaggcgac cattggcgcc aacgccgacc ttgaccatgc cgtcgggcgg gtcggggagg
    14881 cgctcgggct ggtccttcag cgcggtcttc atgtagctga tccagatcgg cagcgcggcc
    14941 ttgccgccgt attcgcggta gccgagcgaa cggaaatcgt cgcggccaac ccagaccgtg
    15001 gtcaccaccg gcccgccgaa gccggagaac caggcgtcgc ggtggttgtt ggtcgagccg
    15061 gtcttgccgc ccacgtcctc gcgaccgagg accttggcgt cggtcgcggt gccgcgcttg
    15121 accacgtcgc gcatcatcga ctgcaactgc caggccacgc gcgggtcgat cgcgcgcggg
    15181 gccacgacca tgccgggatc cagcggcttc tgctcgcgcg gcttcggcgc gtcgggctgc
    15241 ttgccggcgc cggctaccgt ggccccggcg gcaggtgccg tgctggcgac ggtcgggcca
    15301 aggtcgaacc cgtcgacctt cgacggcacc ggcgccgccg tcgcgggctg gcccgggatc
    15361 gcggtcaccg cgcatccggg gcaggcggtc ggtggctttt cggtgaagat gtccttgcca
    15421 tcgcggtcgc gcacgctggc gatgacccag ggcgtggtga ggaagccgcc attggcgaac
    15481 gcggtgtagc cgcgcgctac cgagagcggg gtcagcgagg cggtgcccag cgacatcgac
    15541 aggttgggcg gcagactggc ttcgtcgaag ccgaaatggc tgatgtagcg gcgggcgtat
    15601 tccaccccga tcgcatcgag caggcgcacc gacaccaggt tgcgcgactg caccagcgcc
    15661 tcgcgcacgc gcatcgggcc ggcgaagttg ccgctgtcgt tctgcggacg ccacatatgg
    15721 ccacggcgat ccttgaacac caccggggcg tccagcacga tcgaggccgg gttgaacccg
    15781 cgctcgaacg ccgccgcgta gacgaacggc ttgaagctgg agccgggctg gcggcgtgcc
    15841 tgggtcgcgc ggttgaactt gttgccggcg aagctgaaac caccgaccag cgcccggatc
    15901 gcgccgttgt cggcatccag cgagaccagc gcggcctggc cgcggggcaa ctggtcgatg
    15961 acccattgcg gggcggcgtc ggccttgtcg gagggcaggc ggcgcacgcg caccacgtcg
    16021 ccacgctggg ccaggccgcc ggccgacttg ccggtccagg agatcgcgct gccggcgagc
    16081 cccagttcgc tgccgtcggc caggacgacc cgcaactggc caccttcgct gccggtcacc
    16141 accgcgggca gcaggccgcc ctgtgccggc gtgccgcgca ggaggccggc gatggtcgcg
    16201 gcatccgcgc ccgccgggat ctcgacgcgc tgctccggct tgcgccagcc atggcgatgg
    16261 tcatagacgc gcaggccgtc gatgaccgcc ctgtcggccg cggcctgcag caccggatcg
    16321 atggtggtgg tgacgtggta gcccttgctg agcacgtcgc cgccgaagcg ctcgatcatc
    16381 tgctggcgca ccatttccgc cacgtacggc gcgtacacct cgatctggcg ctcatgcgcg
    16441 gtggcatgca tcggcaccgc acgggccgcg tccgcctcgg cgcggctgac atagcccagt
    16501 tcggccatgc gcggcaggat gtagttgtcg cggcgcatcc gcgcgcgttc cgggttgccc
    16561 agcgggttgc cgctggacgg gaacttgggg atcccggcca gggtggcggc ctcgtccagg
    16621 gtgagctggt ccagggtctt gccgtagtag aactcggccg ccgccgcgac gccgtaggcg
    16681 cgattgccga agaaactctt gttgaggtag agctccagga tctcgtcctt gcccagctcc
    16741 cgctccatct tcatcgccag cagcatttcc gcgaacttgc ggcggaagct gtattcggag
    16801 ctcaggaaga actgcttggc gacctgctgg gtgatggtgg agccgccagc cacccgctcc
    16861 tgcccgccgg tcttcgccag cagccacacg gcccgggcca cgccgttgac gtcgatgccg
    16921 tggtgttcgt agaaattcgc gtcctcggcg gccaggaagg cctgcttgac ccgcgcaggc
    16981 accctggcga tgtcgatcgg atagcggcgc atttccccga acaggcccat caggcgaccg
    17041 tcggcggcat agacgtagag cggttcctgc agctcggtgt cgcgcaggct ctgcacgtcc
    17101 ggcaggcgcg cgctgaccac gtagaccagg atgccgaccg ccagcaatcc gatcaggcaa
    17161 agtgcgaccg cgcccagcaa aaaccagcgc agcatgcggg ggaggcgggg cattggggcg
    17221 aattccgatt gcagccgggc aggccgcgga gtatagagta ggcattcgtg atgccgggcc
    17281 tacagggggc gaccggtttc ccccaaggcc tccgttaacc cgggtgccgg gtgcggtgga
    17341 gaagggtgtt cacggggtgg gaaccatggg gccggttgcc atatcgtgaa aagtccgtta
    17401 ttcatgtact gcagcaccaa gtgcgcgtaa gcgcccgtgg gaaggggaga taccgtgggt
    17461 ctggtgacga aaacacaatc gccgttgatc ggcgtggata tcagttcgac tgcggtcaag
    17521 ctgcttcagc ttgcccgtac cggtgaccgt taccgggtcg agcactacgc cgtggaacca
    17581 ttgccgccga acgcggtggt ggaaaagaac ctggtcgagg tcgaggccgt cggcgaggcg
    17641 atccgccgcg ccgtggcgcg ttccggatca cggatcaagc atgccgccgc cgcggtgtcc
    17701 ggttcctcgg tgatcaccaa ggtgatcccg atgccggccg acctgggcga ggacgacctg
    17761 gaatcccaga tcgaactcga ggcggtcaac tacatcccct acccgatcga ggaagtgaac
    17821 ctcgacttcg aggtcatggg gccgatgccc ggcaacagcg agatggtcca ggtcctgctg
    17881 gcggcctcgc gctcggagaa tgtcgaggtc cgcgcctccg cgctggaact gggcgggctg
    17941 accgcgaagg tgatcgatgt cgaggccttc gccatcgaga atgcctacgc cctgatggca
    18001 ggcagcctga acgtgccccg cgatggcctg gccgcgctgg tcgatgtcgg cgccaccatg
    18061 accacgctca acgtgctccg cggcggtcgc agcatctaca cccgcgagaa cgtgttcggc
    18121 ggcaagcagc tgaccgacga ggtcatgcgc cgctacagcc tgagctacga ggaagcaggc
    18181 ctggccaagc gccagggcgg gttgccggaa agctacgaga tcgaggtcct ggacccgttc
    18241 aaggaagcga tggtccagca ggtcagccgc ctgctgcagt tcttctatgc cggcagcgag
    18301 ttcaatcgcg tcgacagcgt ggtgctggcc ggtggttgcg cctccatccc cggcatcgcc
    18361 gcgatggtcg aggagcagct cgggatcccg accgtggtcg ccaatccgct ggcgcagatg
    18421 accctcggcc cgcgcgtgca ggcacacgca ctggcgcagg acgccccggc gctgatgatt
    18481 gcctgcgggc tcgcattgag gagcttcgac tgatggcacg gatcaatctc cttccgtggc
    18541 gcgcggagcg ccgcaagtcc cggcaaaagg aatttgtcgg catgctgggg ctggcggcgt
    18601 tggccggagt cctggtcgcg ttcctcgtct acagctatta cggcgggcgc atcagcggcc
    18661 agaccgagcg caacgatttc ctgcgcggcg agatcaccaa ggtcgatgcg cagatcaagg
    18721 aaatcgagga actcgaccag aagaagtcca agctgctggc gcgcaaggaa gtcatcgagc
    18781 aattgcagtc caatcgctcg cagatggtcc acctgttcga ttcgctggtg cggaccattc
    18841 cggatggtgt gaccctctcc gccatcaagc aggaaggcga catcctcacg ctgagcggtc
    18901 gctcgcaatc gaacgcgcgg gtatcgacct acatgcggac tctcgaaagc tcgggttgga
    18961 tgaccaaacc cgacctcaac atcatcgagg cgaagggagc gaaccccggg ttgccttacg
    19021 agttcaccct caaggtcacg ctggccaacc cgaacgctcc gaaggaagaa gaaggcgagg
    19081 gtggtgaggc cactgcgcct gccgcagccc cggccgcgcc tgctgcagga ggagccgccc
    19141 cgtgagcaac aacaagaaga tcaacctgaa agagctcgac atcaacaata tcggtggctg
    19201 gcctcgccag gcacagatgg ggttctgcgt gatcgtggcg gtgatcatca tcctgctggg
    19261 ctggtggttg ttcgtgcgcg gccagcagac cgagctcgag aagctggaag gccaggaaaa
    19321 ggaactgcgg accgagttcg agaacaagca ggggcgggcc tccaacctgg agccgctcaa
    19381 gcagcaactg gcgcagatgg aacagcagtt gcagcagatg ctgcgccagc tgcccagcaa
    19441 gaccgagatg ccggacctga tcgtggatat ctcgcagacc gccctggcga ccggcatcca
    19501 gaacgagctg ttccagccgg cgggggagac caagaaggag ttctacgccg agaagccgat
    19561 cgcccttcgc atggtgggga cctaccacca gttcggggcc ttcgtgagcg gcgtggcctc
    19621 gctgccgcgc gtggtgatca tgaccatgca tgacatctcg ctgcagccca aggatgcgaa
    19681 gaccaacccg aatgccagga tcggaccaaa cagcgcgctg gaactggcgg gtaccgtgaa
    19741 aacctaccgt tacctcgacg aagacgagac ggctgcggat gccgcaccgg ccgcaaccga
    19801 caaggcgcct gccaagggca aggccaaggc caaggccgag gccaaagaaa agggaggcgc
    19861 ctgacatgtt cgtgaaagga caccttcgca ccagtcggtg gttgggcatc gcattgctct
    19921 ccaccgcctt ggcggcatgc ggtggcagcc agggcgacct ggagaaatgg gtcgccgaaa
    19981 tcaaggcgcg ccccgcaccg ccgctggatc ccttgcctgt catgcagcag ttcgagactt
    20041 tcgagtacga cgcgcagaac ctgcgcgatc cgttcagcga ggttttcaat ggtggcgaag
    20101 gtagcggtcc ccgcccggat gccgggcgtc gcaagcagac acttgaacag ttcccgctcg
    20161 acagcctcga catggtcggc acgttgggga atggcaaggg cctggtggcc ctggtgatgg
    20221 cccccgacaa ggtgacctac cgggtgcagc ccggcgcgta cctggggcaa agcgatgggc
    20281 gggtgactgc cattaacgaa gaccgggtcg aactggtcga acttgttccg gatggcgcgg
    20341 gtggctggct cgaacggccg gcgaccatct tgctcgaaga caattgattg gggatagacc
    20401 gatgaccgtc accaacgcca agcaacagcg tcctctccgg cgccccctct ccctcggcac
    20461 ctgcgcgatc gggcttgccg tcggcctcta cgccgcgggc gccagcgccc tcccggtcac
    20521 cacgctggcc gcgatggggc agtcggccac cggcgcgatg gccgaccctg ccaagcgcac
    20581 accggatgcc gtcacggttt cctcgatcga cttcaagcgc ggcgatgggg gggcgggcaa
    20641 gctcgtgctc cgcttctccg gtgcgggcgc cgcgccggac ctgcgcaaga cgggcaacag
    20701 cgtggttgtc gatatcggca atgcgcaact gcctgccgcc ctgcagcgcc cgatcaacgt
    20761 gaccgacttc gccacgccgg tgcaacgcat cgacgcgcgt gcgacgagca acggcgccca
    20821 gctcgtgctg ggtacccagg gcgagttcga gtcgatggcc taccagagcg gcaacgaata
    20881 catcgtcgag atcgtgccgc gcgcgagcat ggctggcgtc gccaaaccga aggccgccgc
    20941 tccggcaatg ggcgcttccg cgatgggggc ggccaccagc gcctcgaccg tccggtacag
    21001 cggcaagccg gtgaccttca acttccagga cgtgccggtg cgcacggtcc tgcagctgat
    21061 cgccgaggag tccaacctca atatcgtcgc cgccgacacc gtgcaaggca acgtgacgct
    21121 gcgcctgatc aacgtgccgt gggaccaggc gctggacatc gtgttgcagg ccaagtcgct
    21181 ggacaagcgt cgcagcggca acgtcgtctg ggtggcaccg cagaaggaaa tcgcccagtt
    21241 cgagcaggac aaggaagatg cgcgcatcag ccttgaagag cgtgcggaga aggtcaccga
    21301 atacattccg gtgaattacg ccagcgccga ggacatcgcc aagctcctga ccgaagagag
    21361 caagggcaac agcggcggtg cgagtggcgg cggccaggga ggcggccaga accagcagga
    21421 tcgcggcttc ctctcgcagc gcggcagcct gagctacgac aagcggacca acaccctgct
    21481 ggtggtcgac atcccgcaac gcgtggccaa tatccgcaac ctcgtgcaac agctcgacaa
    21541 gccggtcgac caggtggtga tcgaggcgcg catcgtgatc gccaatgagt ccgtggcccg
    21601 cgagctgggt gcccgtttcg gggtcagcgg caacaacggg gacagctact acagcggcaa
    21661 cctggaagcg aaccagaaca agatcaacgc cgacaaggcg ttggacctgc agttcgcgaa
    21721 ggacatgtcg gcgtggatcg cgggcggcca ggtcggtgcc cgcccggtgt attcgcagtc
    21781 cttgccgttc cgtgggctca tgtccaacct gccggccgca ttgcagaacc cggctggttc
    21841 gctggcactg tcgatcctca acgccggcta ccaactggat gtcgagctgt cggccatcca
    21901 ggagcaggga cgcggcgagg tcatctcaaa ccctcgcatc gtcaccagca accagaagga
    21961 atcggtgatc aggcaaggca aggaaatcgg ctacctgacg atcacgggtt ccggacttgg
    22021 cgccaccccg accgtggcgt tcaaggaagc gctgctcgag ttgaaggtga cgccgaccat
    22081 caccaacgat ggccgcgtct tcctgacctt gggtgtgaag aaggacgagc tggacggtta
    22141 cgtcagcatg ggtaccttcg gcgaagtgcc gcaaatcgcg aagcgtgaag tcaacactgc
    22201 ggtactggtg gatgacgggc agaccgtggt gatcggcggt gtctacgagt tcagcgacac
    22261 caccgaactg tcgaaggtcc cgttcctcgg cgacctgccg gtgatcggca acctcttccg
    22321 caacaagagt cgctccaaga gcaaggccga gctgctggtg ttcgtgaccc cgaaggtcat
    22381 gcgcgttacc cagcgctgag ccacggcgca tcgcacgaca cgaacgggag ccgcaaggct
    22441 cccgtttcgt ttgcggcgaa tgggtcgttc accaggggcg tgcgaacctt tgcgcgttga
    22501 gccggtcgaa gtggcctgca tacgagagcc cccagatgga taaggcaatc cccgacaccg
    22561 ccacgctgca tgacgccttc cgagccctgc gcgaggatct ttcgcgcgag atcgttggcc
    22621 aggccggcct gatcgaacgc ctgctgatcg cgttgctcgc cgatggccac ctgttggtcg
    22681 agggcgcccc cggcctggcc aagaccaccg cgattcgtgc actggcgtca cgcctcgagg
    22741 ccgatttcgc ccgcgtccag ttcactcccg acctgctccc cgcggacctc accggcaccg
    22801 aggtgtggcg cccgcaggat gggcgcttcg aattccagcc ggggccgatc ttccacccga
    22861 tcctgctggc cgacgagatc aaccgcgcgc cggccaaggt gcaatcggcg ctgctggagg
    22921 cgatgggcga gcgccaggtc acggtgggcc ggcagaccta tccgctgccg ccgctgttcc
    22981 tggtgatggc cacccagaac ccgatcgagc aggaaggcac gttcccgttg cccgaagcgc
    23041 agttggaccg cttcctgatg cacgtgcgca tcggctatcc acgcgtcgat gccgaaaccg
    23101 aaatcctgcg gctggcgcgc gaacgcgcgc gcgacgtgct gcaggcggcg tcgcacgcgc
    23161 cggcgcggat gccgcaggcg cacgtgtttg ctgcccgcgc ggcggtgctc gacctgcact
    23221 tcgcccccgc gctggaacgc tacctggtgg agatcgtcct ggcctcgcgc gatgcgggtc
    23281 gctacgacgc cgcgctggca aggcggatcg cgtggggcgc aagcccgcgt ggctcgatcg
    23341 cgctggaacg ttgcgcgcgc gcgcgcgcct ggctggaagg gcgcgacttc gtgaccccgg
    23401 aagacgtgcg tgcagttgcg ccggatgtgc tgcgccatcg cgtgctgccc agctacgaag
    23461 ccaccgcgga aggctgggat ggcgagcgac tggtgtccga gctgttgcgc ctggtgccgc
    23521 tgccgtaagc cggccacgga caggtccgaa cccggagcga ccgtgaccga cggcatcgtc
    23581 cccacgcttt ccgaactggt cggcctgcgc gccctggtgc agggccgtcg cggcgcgcgg
    23641 ctcggccggc atggcgtgca agggcaggcg ctgtccaacc tgcgcgggcg tggcatggag
    23701 tacgcggagt cgcgcgagta cgcgcagggc gacgatgccc gccacatcga ttggcggctg
    23761 accgcacgca gcgggcgaac ccataccaag ctgttccagg ccgagcgcga acgcctcacg
    23821 ctggtcgtcg cggacaccgc gccatcgctg tacttcggga cccgcgtgcg cttcaagtcg
    23881 gtgcaggccg cgcgtgccgg tgcgctggcg gcatggctgg cggcgcgtga cggcgaccga
    23941 atcgccgcgt tgcgcggttc gcatcgggaa gcgccggtgc cccctgcgtc cggaccgcgt
    24001 ggcgccttgc gcgtgctgga tgcactggtg cgctggtatg cgcagccgcc tgcggatgat
    24061 gaaggcctgg cggtcgcgct ggagcatgcg cggcgactgc tccggcccgg ttcgcggctg
    24121 gtgctgctgg ccgatccgtc cagcatcgcc gcagtccccg cggaacgctg gcccgcgctg
    24181 gcgatgcacc atgaattgat cgtgctgctg ttggtcgatc cgctggaaac gtcgccgccg
    24241 gcgaaacgct tgccattccg tggcgcgccg ggcgatcgca tcgaactggg gctggacacg
    24301 gcacccgtgc gccagcgctg gcggcaacaa ttcgacatcc cgttggaggc cacgcaggcg
    24361 atgctgcagc ggcagggagc gcgggtgatg acgctctcca ccatcgatgc cagcgatgcg
    24421 tggctgtcgc tgctgggacg gccgaacgcg gtggtggcga cgggagcggg gcgatgaaca
    24481 tgccgttgcc gctgaaggac gtgcatccgg gcttggcgcc gtcgtggtgg ccgccagcgc
    24541 cgggctggtg gatggtcgcg gctgtgctgg cgctggtggg tgttgcgatg tggtggtgga
    24601 tgcgcaggcg acgcacgcga cgtgcgagca tcctgcgctt gttcgaaggc acggtggatg
    24661 ctgcaacctc gcctgcgcaa caggttgcgg cgatgtcgga cctgttgcgg cgcgcggcac
    24721 ggcgcaagga tccgctcgcg gatcgcttgc agggcgacga ctggctccgc ttcctcgacc
    24781 agggaatgac cccgccatgc ttcgagcatg gaccgggtgc gctgctgcgc gagggtgcat
    24841 tccgtcgcga tgtcgatgaa ggcgcggtac gcgcgctgcg aaccgtcgcg cgccagcgct
    24901 acctgacctg gatgctcggc aagtgaacct gctcgcggac tggctgggca tcgacggctt
    24961 cgcctggccg tggctgttgc tcgcgattcc cttgccgttg ttggcgcgtg tgttgatgcc
    25021 ggcgcgtgcg aacccgcaac cagcgctccg cgtgccctgg ggcgagcgcc tgcgcagggt
    25081 ggcctcgggc ggcgtgctgg aggtgggttc gcgcggcttc ccgtggatgg cgatgctggc
    25141 ctggtgcctg ctgtgtgtcg ccgcggcgcg cccgcagcag ttgggtccgc ccatcgcccc
    25201 gccgcaggtg ggccgcgaca tgatgctggc cgtcgacctg tccgccagca tgggcgagga
    25261 agacatggaa cttggcggtc gcctggttga tcgcctcacg gcggccaagg cggtgctggc
    25321 cgatttcctc gaccgtcggg tcggtgaccg catcggcctg gtcgtgttcg gcgaccgtgc
    25381 cttcgcgctg acgccgttga cgctggatcg cgacagcgtg cgccagcaat tggatgccag
    25441 cgtggtcggc ctggcggggc gcgcgactgc gctcggtgat gccatcgcgc tgtcgaccaa
    25501 acggctgcaa cagcaacgca ccgagcagcg cgtgctgatc gtgctcacgg atggcgtgaa
    25561 caccgccggc gtcctggagc cagccaaggc cgcgcagatc gcgcgcgatg ccggcgtgcg
    25621 catccacgcg atcgccttcg gcggtgaagg cggcggcgcg ctgtcgctgt tcggcctgcg
    25681 cctgccggcg ggcggcgacg aggtcgatga agccggcctg cagaagatcg cccagctcac
    25741 cggcgggcgt ttcttccggg cccgcgacac cgaggcgctg gccggcatct acgcggagat
    25801 cgacgcgctg gaaccggtcc agcggcaggg acaggcggtg cgtccgaaga tcgagtgcta
    25861 tccgtggcca ctcggacttg ccctggcggt cggcgcgctg gccgtgctgc tgcggaggcg
    25921 ggcatgaacc tgttccctga cgtgcatttc ctgcgcccgt ggtggttgct ggcgctgctg
    25981 gtgttgccga tgctcgcttg gtggtggcgc aggcaggcgc aacagcgcaa tccttggcgc
    26041 gacctggtcg atgcgcattt gttgccgcac ctgctggacc gcggtgccga gcaacgcgca
    26101 ggggttttgc cgcgttggtt ggcattggcc gcaaccgcgc tcgcgatcat tgcgctggcc
    26161 ggccccggca tcggcaagga cgaacagccg ctgtggcagg cgcaggaacc gctggtgatc
    26221 gcgctggacc tgtccagcgc caccctggcg cgcgacctgc cgccgacgcg gcttgcgcag
    26281 gcgcgcgcaa agctggccac gttgctgcgc gaacgcgccg gtggccaggt ggcgctggtc
    26341 gcgttcgccg gcgatgcgtt cacggtggcg cctctgaccg aggacgcagc caacgtcgcg
    26401 ctgttcctgg atgcgctgga tcccggggtg atgccggatg acggccatcg cgccgatcgc
    26461 gccatcgcgt ggtcgcaggg actgatgcgc caggccggat tcgcgcgtgg gcagatcctg
    26521 ttgcttaccg accgcgccga cggcaccgat atcgatgccg cgagcaaggc gaacgccgcg
    26581 ggctaccgcg tgtcggtgct gggcctcggg actgagcagg gcggcgtgtt cgatacgccg
    26641 gagggtctcg gccaggcgcg gctggacgcc tccgggttga agcgcctcgc ggccagcggt
    26701 ggcggcggtt accaggcgct gtccccgggc gatgccgacc tccgcgcgct gggcgtgctg
    26761 gatccgcggc aggacgccgg taccagcgtg cgcgggcaga aggtgtcgcg ctggcgcgac
    26821 gacggctact ggctgcttcc cgtggtcttg ctgctgtgcc tgccgctgtt ccgtcgcggc
    26881 gctgcattcg cgctgctgct cgcttgcggc ctgtggctgc cgatggtgcc tgcgtccgca
    26941 cagtcatcgt cgtccggagg cagcctgtgg caacgcgcgg accaggccga acatgcgcgc
    27001 atgcaggacg gcatcgcggc ttacaggcag aaggactatc cgcgcgcgat cgaacggttt
    27061 tcgaccgtcg gcggcgcgga aggccagtac aacctgggca acgcgcttgc caaggcgggc
    27121 cgctacgacg acgcgatcgc cgcctacgat cgcgcgctgg aattgcagcc gggaatgaag
    27181 gacgcagcgt tcaaccgcaa tctggtgcgg gtggcgaaac aactgcccaa acgcccgcag
    27241 gacaagggcg aggacaagga cggcaagggc ggcaaggacg ggcagccccc gcaggcatcg
    27301 aagccgggac agcaaggcca ggcgggcaag ccgcaagcac cgccagcgca aggcaagggc
    27361 ggccaggcag gcaagccgcc gcaagcgccc ccgcagaagg atccgcaatc gcaagcgcag
    27421 gccgacgccg cgcaacgcga acgcatgcag caggcgttgc agaagcaaca ggcgaagcag
    27481 cctggccagc aaccgaagcc cgaccaacgc ggcaagcccc aggaaaccgc cgagcagcgc
    27541 gagcgtcgcc tcgccaacga agcgtggttg cagcgcgtgc ctgacgatcc gggtgcgttg
    27601 ctgcgtgcgc gcttccaact ggaacagcag cgccgtcgcg gggccacgcc atgacgcgcg
    27661 cgatcgcgtg gctgttggtg tgcctgctcg cgtttttcgc gtcgccatcg catgcacaga
    27721 cacgcgcctg gctggatcgc gcgcaggtca gccatggcga aaccgccacc ctcaacatcc
    27781 agaccgacca gtcggtggcg tcgatcgact acacgccgct gcaacggcag ttcgacatcg
    27841 cgggacagac cgtgcgacgc agctatcaac gcagcaatgg acgctcgacc gcgacctcgc
    27901 tgttcgctgt cggcctgcgc ccgcgcggcc ccggggtctt caccattccg gcgttgcgcg
    27961 tcggcaagac caccaccgcg ccgttgcgat tgacggtggt gccgccctcg gtgctgcccg
    28021 ccagcggcga cgcggacgcc ttcgtcgaga ccgtggtcga tgccgcgcgg ccctacgtgc
    28081 agcaggcggt cggggtgcag gtgcgcctgc actacgcggt gccgctgatg tccggacagc
    28141 ttgaacagga tccgccgacc ggggccagcc tgcgccaggt cggcgaggac ctcacctacc
    28201 agcggcagat cgacgggcgc cgctacaacg tggtcgagcg gcggttcctg ctgatcccgg
    28261 agcgcagcgg tgcattggtc ttgcccggcg cgcgcttcaa cggacagacc atgggcggct
    28321 tcttcgacca ggtgttcgat gacggccgca aggcggtgtc cgcggcctcg ccagcccggc
    28381 gcctcgacgt gctgccgatt cccacggggg ccccgcagcc gtggttgccg ctgcatgacc
    28441 tcaagttgcg atatgtgcag gcgccgacgc aggcgcgcgc gggtgaagcg gcatccgtcg
    28501 agatcgaagt catcgccgac ggcgccaccg ccacgcaatt gccgccggtc gcgctggcgg
    28561 agacggcggg cgtgcaattg ttcgcggatc cgccgcaggt tgacgagcaa ttcgtcgatg
    28621 gccggccgcg cagcatcctg cgccggcgca tcgccatcgt gcccttgcag gccggggcgc
    28681 tgtcgatcgc ggggccgcgc atcgaatggt gggacgcggc gcagggcgtg gcgcgtaccg
    28741 cgatgctgcc gtcgttgcag ttgcaggtcg cgccgggcgc cgctgccgcc acgacctccg
    28801 tcgatgccgc ggcacccgcc gggcgtgcag gcgcggatgc gccatcgggc gatgccgcgc
    28861 atgcgtggtc gcgcaggtgg ccgtggctgc tgctcgccgc ggcgttgctg gtcgtgctcg
    28921 cgtggtggcg ttggccgcgc gcgcgggtgc ctgcagcgga tgcatgcggt gatgcgccat
    28981 cgcccgaggc accggcagcg cgcatcccgt cgttgccgga cgcactgaag cgtgacgacc
    29041 tgggcgtgat cgcgcaggcc ctgggcgcgg cggccggctc gcatggcgag gatctcgacg
    29101 ccgtgcgcca gcggctggac gatgcggcgc aggtcgaggc ggtcatgcag ctgcaggccg
    29161 cgcgctgggg cagcggcgat gccgcaccga ccctcgccgt gttgcgtgcc gcgttcgcga
    29221 agggcccgcg ttggcgcaag gctgcgccgg catcgaactc gttgctgcca ccgctgtatc
    29281 cggaataggt gcgcagcccc ttcgacttcg tggcttcgcc acgacggtca gggcggacaa
    29341 gctcggtttg ttaagctcgc ccgctacacg gagcgaggcg acgcgatgag cgaggcgaag
    29401 gccggaagcg gcaagctgca actgcactgg aagatgctga tcggcttcct tgccggcctg
    29461 gcgcttggcc tggtcgcgca ctacggtgcg ggcagcgacg ccggctgggt gcagtggctc
    29521 acgacctacg tgacgcaacc ggcgggcacc ttgttcctgc gcctgatctt catgctggtg
    29581 atcccgctgc tgttctccgc gctgatcatc ggcgtggccg aaatgggcga tgtgcgctcg
    29641 ctcggccgcg tcggctggaa gacgctgggc tacacggtga tcgcgtcggg catcgcggtc
    29701 gcactgggcc tgctgctggt gaactggctg caacccggcg cgggcgtgga tccggccgcc
    29761 gcgcaggcga tgctggcgca gggcgcggac cgggcgaagg acatcgtggg cagcactgct
    29821 tcgcagcccc ggggcctgga catgctgctg gcgatcgtgc cggacaacgt ggtcaaggcc
    29881 gcggccgaga acaccatcct ggcggtgatg ttcttcgcac tgatgctcgg catcggcctg
    29941 gtgctgaccc gcacgcccgc caccgaaacc ctgctgcgcg gcatccaggg cctgttcgag
    30001 gtgtcgatga cgctgatcgg gctggtcatc cgcctggcgc cgtacgcggt gttctgcttc
    30061 atgttcaacc tggccgcgct gttcggctgg gacctgctca acagcctggg caaatatgtc
    30121 ggcgtcgtgg tcctggcgct ggcgctgcac atgctcgtgg tctactcgtt gatgctgaag
    30181 ttcctcggcg gctggtcgcc gctgaagttc ttcaagggct cgcaggaagc gatggtgatg
    30241 gcgttctcga ccgcgtcgag caacgcgacg ttgccggtgt cgctgcgggt cgccgaggaa
    30301 aaactgcacc tgccgaagaa aatctcgcgc ttcgtgctga ccgtcggcgc caccgccaac
    30361 cagaacggca ccgcgctgtt cgagggcgtc accgtgctgt tcctcgccca gttcttcggc
    30421 gtggatctct ccctcaccca gcaggtcacg gtgatgttcg tctgcatcct cggcggcatc
    30481 ggcaccgccg gcgtccccgc tggctcgctg cctgtggtgg cgttgatctg cggcatggtc
    30541 ggcgtgccgc cggaaggcat cggcctgatc ctgggcgtgg accgtttcct cgacatgtgc
    30601 cgcaccaccc tgaacgtgac cggggacctg atgctggcgg cagtggtgtc gaagggcgag
    30661 gaagacccgg agaccgcgta gccacgcgtt cttcgctcgc gtttccggat cgggctgcgc
    30721 gcatgggaca atgggcggcc cgcatgccgc cgggcgcctc cccatgatgc ccccatgacg
    30781 acgcccagcc gccgcgacct cgccaacgcc atccgtttcc tcgccatcga cgcggtccag
    30841 gccgccaact ccggccatcc cggcatgccc atgggcatgg ccgacatcgc cgaagtgctg
    30901 tggaacgatg tcctcaagca caatccggcg aacccgcgct gggccgatcg cgaccgcttc
    30961 atcctgtcca acggccacgg ctcgatgctg cagtacgcgc tgctgcacct gagcggctac
    31021 gacctgtcga tcgacgacct caggcagttc cgccagctgg actcgaagac cccgggccat
    31081 cccgagaact tcatgacctg cggcatcgag accaccaccg gcccgctggg ccagggcctg
    31141 gcgaatgccg tgggcttcgc gctggcggag aaactgctgg cgcagcgctt caaccgcgat
    31201 ggccacgcga tcgtcgacca ccgcacctgg gtgttcatgg gcgacggctg cctgatggaa
    31261 ggcatcagcc acgaagccgc atcgcttgcc ggcacctggg gcctcggcaa gctggtcgcg
    31321 ttctgggacg acaaccacat cagcatcgac ggcaataccg atggctggtt caccgacgac
    31381 accccgaagc gtttcgaggc ctacggctgg aacgtggtgc gcggcgtcga cgggcatgac
    31441 ccggtcgaga tcaggaccgc gatcgaaacc gcacgcaagt cctccgacaa gcccacgctg
    31501 atctgctgcc gcaccacgat cggtttcggg tcgccgaaca aggccggcaa ggagtcctcg
    31561 catggctcgc cgctgggcaa ggacgaagtg gtcgccaccc gtgaagcgct gggttgggaa
    31621 cacggcgcgt tcgacatccc gcaggcgctg tacgacggct ggcgcgcacg cgacctcggc
    31681 gcggtcgagg ccgactggga cgcgcgtttc gctgcgtatg ccgcggcatt cccgtccgaa
    31741 gccgccgagt tccagcgtcg ccttgccggc gacttgccgg acggcttcgt cgatgccgcg
    31801 ctggcgttcg caatcaagac ccagcaggac ggcccgagca tcgccagccg caaggcctcg
    31861 cagaactgca tcgaggcgtt cgcgccgctg ctgccggaac tggtgggcgg ttcggccgac
    31921 ctggcgcatt ccaacctgac cctgtggaag ggcagcaagt cggtcgcgac cgatgatgcc
    31981 aacgccaatt acgtctacta cggcgtgcgc gagttcggca tgaccgcgat cgccaacggg
    32041 ctcggcctgc atggcggctt catcccgtac gacgccacct tcctggtgtt cagcgattac
    32101 gcgcgcaatg cggtacgcat ggccgcgctg atgggcgtgc gcgccctgca tgtctacacc
    32161 cacgattcga tcggcctggg cgaagacggc ccgacccacc agccgatcga gcacctcgcc
    32221 tcgctgcgct acatccccga caacgacgtc tggcgtccct gcgatgcggt ggaatcggcg
    32281 atggcgtgga aggccgcgat ccagcgcgat gatggtccga gctgcctgat cttcagccgc
    32341 cagaacctgc cgcaccaggc gcgcgacgaa ggccagttgc tggacatcgc gcgcggtggc
    32401 tacgtgctga aggacagcgt gggcacgccg gaggtgatcc tgatcgcgac gggttcggaa
    32461 gtgggcctcg cgatgcaggc cgccgagcag ctgggcgacg gcgtgcgcgt ggtgtcgatg
    32521 ccgtgcaccg acgtgttcga tcgccaggac gcggcgtatc gcgaatccgt gctgccgaag
    32581 gcggtgcgca agcgcgtcgc catcgaggcc ggcgtcagcg atttctggcg caagtacgtc
    32641 ggcctggatg gcgcggtgat cggcatcgac acgtttggtg cctcggcgcc gatcgaggca
    32701 ttgatgccgc acttcggttt caccgtcgag catttgatcc aggtcatccg cgaactctga
    32761 gggccgcgct agcatccccg ctgtcatgtc gatccacagt cgccccctga accgttggat
    32821 ggcccgcctc gcgctggcgg cgatcctgct gctggccggc gtgccgacac tcgggcgctg
    32881 gatggacgcg ggccgcgagg ccccggcgct cgctgccacg gccatgtgca ccagcgaggg
    32941 catggcctgg tcggcgcctt ccagtttgtt cggaagcgat ccgattcccg ccggtaccca
    33001 aaccggcgag gactgcgcct actgcccgtt gctggccagc gccgtgccgc tggtgctggc
    33061 gacgctactg ctgtcaccac cgccggcgcc gccgcccgcg gtcgacatcc ccgtagcgcc
    33121 agcgagcgat gcggggcatg cgcatggact gggtgcacgg ggtccaccgc ttttctcctg
    33181 aaccacaaga ccgtctttcc cgcgaggccg cgtgcctcgc cccttgtgtt ccggagccaa
    33241 catgtcccgt ttcccgcttg cgacggcgct gccgtcgcga ctggcgcttg cctgcgccat
    33301 ttccctggcc gcctttccgg ccgctgcctc cgatgacgag gcgaagacgc tggatcgcgt
    33361 catcgtcacc gccacgccga tccagccgct gaccttcgaa accgacccga agctgccgcg
    33421 ccagccggtt cctgccagcg atggcgccga ctacctcaag accattcccg gcttcaccgc
    33481 cctgcgcaat ggcggcacca acggcgatcc ggtcctgcgc gggatgttcg ggtcgcgcct
    33541 caacctgctg tccaacgatg gcagcatgcc cggcgcatgc ccggcgcgga tggacaaccc
    33601 gctgtcctac gtcgccccgg agacctacga ccgcctgacc gtggtgaagg gcccgcagac
    33661 cgtgctctgg gggcctggcg cgtcggccgg gacggtgcgc ttccagcgcg acacgccgcg
//
LOCUS       DAOOCX010000011        32499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1031_length_32499_cov_8.215017, whole genome shotgun sequence.
ACCESSION   DAOOCX010000011 DAOOCX010000000
VERSION     DAOOCX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 32499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32499
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1031_length_32499_cov_8.215017"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PK399_01945"
     CDS             <1..715
                     /locus_tag="PK399_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPW11922.1"
                     /translation="ATLEGRALHNVQNAMFAASIAFSLGMKLDAIRHGLRTFDTTFFQ
                     APGRMNVYGEHPFKVIMDYAHNAHATSVMADLAQRLDVGGRRIVVVAGPGDRRDEDLR
                     DIAATVAGKFDHYIVRRDDGLRGRDGDEVPRIIARALEAAGVDASAISMIPDEQEAVD
                     AALRMGQPGDLLVVFADALVRTWKQIIRFKPEGEMQTSTARAEVPTLETTLDEQLVAA
                     MEGVVRDERGLRFEREESD"
     gene            712..2490
                     /locus_tag="PK399_01950"
     CDS             712..2490
                     /locus_tag="PK399_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPW11923.1"
                     /translation="MSTVAMQEPFEDSRRLTGANLYFDGSGAALETAHGLTFDAGTPQ
                     RWRANIHKALALLGWPLGAVVVREHASGASLAFEAPFDQLYVATEVNEWALYSAMGLR
                     AGDTPVDDEAELPRPHVAHHDDDEALRQLQALAAAEAKPALRALVEDAHARGVPAHAD
                     DDALSLGEGATSQRWPLAGLPATDDVPWARLHAVPKAVVTGSNGKTTTVRLLAAMLRA
                     HGLHAGYSSTDGLFIDGERVEAGDYSGPVGARTVLRDARVDAAVLETARGGLLRRGLV
                     ARDARVAVVTNIAADHFGEYGIHTLDHIAAVKLVVAKSLAADGLLVLNADDPMLVAHA
                     PASRRIGWFALALADAMARGTHACGVRDGRLALSHDGIEHDLGEVAAMPLSVGGAARY
                     NIANIAAASLAAHALGIAASTIAGVLARFGARNADNPGRLQRWTLGDVDVLLDYAHNP
                     DGLRGLLDVAAGLRRGGRLGLLLGHAGNRLESDFHALAAVAADAKPERVWLKDIGGDY
                     LRGRASGDVAMILRDALLASGMAETDLPVCLDEAQASREALAWARAGDLLVLPVHELA
                     RRDEVVALLDHLQAEGWRAGEVLPVA"
     gene            2655..3002
                     /locus_tag="PK399_01955"
     CDS             2655..3002
                     /locus_tag="PK399_01955"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPW11924.1"
                     /translation="MHDDRFARLPEPPYYAVIFSSRRRGDAAGGYAEAATRMEELVAT
                     QPGFLGAESARDGEGFGITTAYFDSEASIKAWREHAEHASTRQRGREAWYSQYAVRVA
                     KVERAYGWERADA"
     gene            2995..3786
                     /gene="trpC"
                     /locus_tag="PK399_01960"
     CDS             2995..3786
                     /gene="trpC"
                     /locus_tag="PK399_01960"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPW11925.1"
                     /translation="MHDVLKRILARKQEEVAERRERVSLFELKARAASAPPVRGFADA
                     VQAKIAAGLPAVIAEVKKASPSKGVIRADFHPADIARSYEAGGAACLSVLTDIDFFQG
                     SDAYLQQARAACSLPVLRKDFIVDAYQLHEARVLGADCVLLIAAALDDRQLSEFAFIA
                     DELGMDVLVEVHDLDELERSLPVPARLLGINNRNLRTFEVSLQATLDLKAMVPADRVL
                     VTESGILAPADVQLMRQADVHAFLVGEAFMRQPDPGRALSDLFAA"
     gene            3789..4520
                     /locus_tag="PK399_01965"
     CDS             3789..4520
                     /locus_tag="PK399_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HPW11926.1"
                     /translation="MHEAASAFPVPRDDAPLVVFDFDHTLYDGDSGSHLFKWLIQRAW
                     WRQLLAILAAPVAGPMVAFLPTRRAGISVFVWIGTIGLHRRRDLDALIDAYVAGHVDA
                     IRSRLLPIALEVFRAHREAGDRVVVATGAPPELARAILAFVAHEDVPVVGTLVGPKFG
                     AVAAIRHCHHAMKMTMLRDAGFTRPVAIAYTDSSADLPLLQAAVRPVVVNPKAGSVAM
                     FRKVLPAGTPILNWGCADRAGDPVA"
     gene            complement(4593..5060)
                     /locus_tag="PK399_01970"
     CDS             complement(4593..5060)
                     /locus_tag="PK399_01970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW11927.1"
                     /translation="MSQAFSSGPSPLGTDLRSAMIADADDVARLLTDLGYPCEIEDAA
                     ERIDAIAANDRQALVLARRDGAVCGLVALDFMYYLPLGTITCRVTALVVTPTAQGLGI
                     GRQLLKEAERRARSGGAARIELTSGSQRTEAHAFYRACGYKDSSVRFVKQLGA"
     gene            complement(5068..5751)
                     /gene="crp"
                     /locus_tag="PK399_01975"
     CDS             complement(5068..5751)
                     /gene="crp"
                     /locus_tag="PK399_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HPW11928.1"
                     /translation="MSTSYSKPVMPRAINPLLADAATIERFIAHCHRRKYPARTDVFR
                     PGDDAGTLYYIMSGSVSIIASEDDGRELVLGYIGPGEFVGEMGLFVEAQQRGVALRTR
                     TACELAEIGYERLQQLLTTQANDAARLLYSMGAQISQRLLDTSRKAGRLAFLDVSDRI
                     YRTLFDLSREPDALSHPQGTQLRVSRQELARLVGCSREMAGRVLKKLQADGKLHARGK
                     TVVVYGTRD"
     gene            5876..6667
                     /gene="speD"
                     /locus_tag="PK399_01980"
     CDS             5876..6667
                     /gene="speD"
                     /locus_tag="PK399_01980"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPW11929.1"
                     /translation="MVKPLPRLKLQGFNNLTKSLSFNIYDVCYAASEDERRRYIEYID
                     EEYNADRLTQILTDVAEIIGANILNIARQDYDPQGASVTILISEQPVVDKADAKGVIS
                     DAVVAHLDKSHITVHTYPETHPDNGIATFRADIDVSTCGVISPLKALNYLIESLESDI
                     VVMDYRVRGFTRDVKGRKHYIDHKINSIQDYLAKNVGARYEMLDVNVYQENIFHTKMH
                     MKEFDLDNYLFEEKAKNLSFKERMKIEARLKREIEELYHGRNLVD"
     gene            complement(6726..7259)
                     /locus_tag="PK399_01985"
     CDS             complement(6726..7259)
                     /locus_tag="PK399_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11930.1"
                     /translation="MAAHEYDPDDNFEHAFHAQAGVEEEDALEAVAWQFLLLVNPDDE
                     DAALEQFAAFQRALDEAGERADPALLLRDATDWKAGFHVGEDDAQGLMEALDELAARW
                     RLRIDWPLDEDAGEAPDTSALLHAAFVQLREHHFCLWTVETGEPTLAGWITRERDMEA
                     MQLVAGALGLPARPGAG"
     gene            7379..7939
                     /locus_tag="PK399_01990"
     CDS             7379..7939
                     /locus_tag="PK399_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HPW11931.1"
                     /translation="MAANATIYKAELQVTDLDRHYYASHALTLAQHPSETETRLLVRL
                     IAFALHADERLEFGRGLSDEDEPALWRRDYTGDIEQWIELGQPDEGRLRKASGRAAQV
                     VVVGYGGQAAEAWWKRNAAALGRLRNLTVLELDDAEVAAATDLLGRGMRVTAMIQDGE
                     LQLMDAERSVSLRPRIRLQHGVATPD"
     gene            complement(7946..8590)
                     /gene="coq7"
                     /locus_tag="PK399_01995"
     CDS             complement(7946..8590)
                     /gene="coq7"
                     /locus_tag="PK399_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HPW11932.1"
                     /translation="MSTSRILSRLDRLLDSAQNALSTVAGSPRAGRANPGESTPDVVL
                     DDAERRHAAGLMRINHVGEVCAQALYIGQAAVARDPATRAHLLEAAQEETDHLAWCAD
                     RLRELDSRPSLLNPVWYAGSYAIGALAGLRGDGWNLGFVVETEHQVEAHLEEHLHTLP
                     DADLRSRAILQVMKDDEARHADQAEQAGAKVLPQPVPALMKAASTLMKAVAYRL"
     gene            8784..9212
                     /gene="rplM"
                     /locus_tag="PK399_02000"
     CDS             8784..9212
                     /gene="rplM"
                     /locus_tag="PK399_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPW11933.1"
                     /translation="MKTFIAKNETVQRDWYVVDAEGKTLGRLAAALAYRLRGKHKPVF
                     TPHVDTGDYLVVINAEKIAVTGKKLTDKMYHRFTGYVGNLKTESLQQALERHPERVIE
                     IAVKGMLPKNTLGRAMYRKLKVYKGSEHPHAAQQPQTLDI"
     gene            9215..9607
                     /gene="rpsI"
                     /locus_tag="PK399_02005"
     CDS             9215..9607
                     /gene="rpsI"
                     /locus_tag="PK399_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPW11934.1"
                     /translation="MAISQNYGTGRRKSSVARVFLRKGTGTITVNERPLDEFFGRETA
                     RMIVRQPLELTKHIETFDIVATTTGGGTTGQAGAIRLGIARALVEYDETLKIELRKAG
                     FMTRDAREVERKKVGLHKARRATQFSKR"
     gene            9755..9829
                     /locus_tag="PK399_02010"
     tRNA            9755..9829
                     /locus_tag="PK399_02010"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9787..9789,aa:Gln,seq:ctg)
     gene            9948..10685
                     /locus_tag="PK399_02015"
     CDS             9948..10685
                     /locus_tag="PK399_02015"
                     /inference="COORDINATES: protein motif:HMM:NF021527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HPW11935.1"
                     /translation="MVAHFPAPSVPLDDLDRALRDDGYAVLEPASVVALAGVGAGGFD
                     GLLPSWDDLPQDDYLKDGGRYRKRRHASFIAEAGALVQVPPRRHWQPVEYNALHGGME
                     RWFAPVTPEVMAHPAWSKLVLAIADACSRCKPAPRWHVEAHQFRIDTTDGLGRPTPEG
                     AHRDGVDFVAVLLVSRSGIKGGETRVFDANGPRGQRFTLDAPWSMLLMDDARVIHEST
                     PIQPIGTFGHRDTLVLTYRAAAFLGDD"
     gene            10866..11855
                     /locus_tag="PK399_02020"
     CDS             10866..11855
                     /locus_tag="PK399_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPW11936.1"
                     /translation="MKLGSLKEGGRDGTLVVVSRDLTKAVRATGIAPTLQRALEDWSN
                     TAPRLNALAESLDAGDADGVFDLDMQALAAPLPRAYEFVDGSAYLPHVARVRKARGAE
                     VPESFYVDPLMYQGVSAGFYGPRDAVKVVSEDYGIDLEAEIVIVTDDVPMAVTPEQAA
                     SHIQLIGLVNDVSLRNLIPNELAKGFGFLQSKPRSALSPVFVTPDELGAAWRDNKLHL
                     PLVTHINGTWFGAPEAGVDMQFDFAQLVAHAAKTRPLSAGTIVGSGTVANEDTSLGAS
                     CFAEQRTVETLRDGKPSTPFMAFGDLVRIEVLDAAGNSVFGAIEQRIERQDIA"
     gene            11956..12621
                     /gene="maiA"
                     /locus_tag="PK399_02025"
     CDS             11956..12621
                     /gene="maiA"
                     /locus_tag="PK399_02025"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HPW11937.1"
                     /translation="MTETLQLYSYWRSSAAYRVRIGLNLKGLAHSIVPVHLLRDGGQQ
                     HSAGYREINPQELVPTLCHGQRRLGQSLAILEYLDEFWPEPPLLPANARARHRARALA
                     QLVACDVHPLNNLRVLQYFEQEWNVPQPERDEWVRHWIVEGFRAAEAMLADHPSTGIF
                     CEGDIPGLADCCLVPQVYNARRFGVDMAAYPTIERIESACLALPAFDAARPERQPDAP
                     APT"
     gene            complement(12702..13814)
                     /locus_tag="PK399_02030"
     CDS             complement(12702..13814)
                     /locus_tag="PK399_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPW11938.1"
                     /translation="MDIGYFLKLMAEKNASDMFLTSGAPVNIKVEGDLLPLGSAPLPT
                     GMSKKVAYSLMNEAQIAEFERELELNMAIAVAGSGRFRVNVFMQRGEVGMVIRAIRSD
                     IPSIEELQLPQVLKDIIMARRGLVLIVGSTGSGKSTTLASMIDHRNSTSTGHILTIED
                     PIEYLHRHKMSIVNQREVGLDTHTFHAALKNAMREAPDVILIGEIRDAATMEAAIAFA
                     ETGHICLATLHSNNADQTIERILNFFPEGAHKNVLMNLALNLKAVVSQRLVVGVDGRR
                     MPATEVLINTPMIRDLLRRGQVHEIKMAMEESLQEGMQSFDQCLFRLYKEGRIEMEEA
                     LKAADSRDGLALKFRLSEGGGAEHDPYADVFGSGSH"
     gene            13905..15167
                     /locus_tag="PK399_02035"
     CDS             13905..15167
                     /locus_tag="PK399_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW11939.1"
                     /translation="MQTSFAHFRRALQAPLAGLVLVIAGCATLPPPTAELDAAQQAVA
                     RAEAADAEQYAADELARARDALSRAQAAMARGRDAEAREAALAAGADADLARVRSSAE
                     TTRAEFRQQQEGISGLRARLQMDAAPMQALPFGEPMAGAPPAQRLQALDADPRLNPFA
                     QYERLQARQALAAVDTASRSKQPAALALADRRVAIAEQAARIEATRREIDRLERERSE
                     LLVEASRRDAERARAEAERLRIEAQVQAEEAARLRAQAEQAEAVLDSAQIDQGAKVAA
                     AREKEAALARQEAELVAGAKLPPSRRDARGDVFTLAGDAFASGQAQLTAGAAASIKAL
                     GIYLAAVPGGAVQVLGHTDSQGEEAANQALSERRAQQVRATLVAAGLARGRVQAQGVG
                     EAQPVADNTTAAGRAKNRRVEVVVADNP"
     gene            15598..15810
                     /locus_tag="PK399_02040"
     CDS             15598..15810
                     /locus_tag="PK399_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HPW11940.1"
                     /translation="MFEGQPQTELEAKMKASPEFRQLYYRHQELDKQVLDAELGVLPI
                     NDMTLGQMKREKLAAKDRLLRMYDTH"
     gene            15946..17322
                     /locus_tag="PK399_02045"
     CDS             15946..17322
                     /locus_tag="PK399_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPW11941.1"
                     /translation="MATHDSVLELVGDTPIIRARHLDVGPCTLYLKLESQNPGGSIKD
                     RIGLSMIEAAEKRGDIQPGGTLVEGTAGNTGIGLALAAQQKGYKLVLVVPDKMSREKI
                     FNLKAMGAQVVLTRSDVAKGHPDYYQDMAERIARETPGAYFINQFGNPDNPAAHEFGT
                     GPEILAQMADVGGLDAIVFGCGSSGTMTGLSRCFATHSPHTELVLADPVGSILEEYIN
                     RGTLSEKSASWMVEGIGEDFLPAIADFSRVAKAYAITDKESFLTARELLAKEGILGGS
                     SSGTLLAAALRYCREQTSPKNVLVFVCDTGNKYLSKMYNDFWMLDNGFLEREQHGDLR
                     DLILRPFAARDTVVVGPNDLLITAWQRMKLYDVSQLPVMEGDRIVGIVDESDVLLHVY
                     GDEARFRDPVATAMVSKLDKVNVGAPIEALLPVFDRGHVAIVMEGERFIGLITRIDLL
                     NYLRRRVQ"
     gene            17341..18501
                     /locus_tag="PK399_02050"
     CDS             17341..18501
                     /locus_tag="PK399_02050"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HPW11942.1"
                     /translation="MSDNNDHGLGTLAIHGGQSPDPTTGAVMQPIYATSTYAQSSPGV
                     HQGFEYSRTHNPTRFAWERGVASLEGGSRGFAFASGLAATSTLLELLDSGEHVIAMDD
                     LYGGSYRLFERVRTRSAGLSFSFVDLTDPAAFEAAITAKTKLVWIETPTNPLLKIVDI
                     AAIAAIAKRHGLLVAVDNTFASPMLQRPLEHGADIVMHSATKYLNGHSDMVGGMLVVG
                     ADAELGEQLAFLQNSVGAVQGPFDSFLALRGLKTLHLRMRAHCANAQALAEWLQGHAG
                     VVDVIYPGLASHPHHDLAKRQMDGFGGIVSVRIKGGFEGAKRFCERLDLFTLAESLGG
                     VESLANHPAVMTHASIPPARRAELGISDDLVRLSVGVEELADLQRDLEHALG"
     gene            complement(18505..19278)
                     /locus_tag="PK399_02055"
     CDS             complement(18505..19278)
                     /locus_tag="PK399_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPW11943.1"
                     /translation="MAPRSPEPRIAFLVGGAQKAGTSALAQYLRACPDLRLPADKEAH
                     VFDAPDFDDAWSTGDIDARLAQQFPDAPGDVRWGDATPFYLFHPRCVARIARYNPAMR
                     WIVLLRDPVERARSHHHMERQRGHESLPLWLAAWLEPWRVRGHADDLARGSRLRRFSY
                     RARGDYARQLDVLYAHFPRGQVLLLRSERLRNDPAACVREACTFLGVSPPPPGTGYAP
                     VFVGDYPPASPSAFESRFLRWLMRRERRAMRERHGIAFD"
     gene            complement(19259..20578)
                     /locus_tag="PK399_02060"
     CDS             complement(19259..20578)
                     /locus_tag="PK399_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HPW11944.1"
                     /translation="MPAAAASTSGSAPVILVRDVCKTFRTWATPAQRLIAPALHRAGN
                     LLAQAAPRLSDRLHAAAHRRMHVHEALHGIDFELHRGEALGIIGLNGSGKSTLLQIIA
                     GVLPPTSGRVETHGRVAALLELGSGFNPELTGRENIYINGAILGLATARIDALLGDII
                     AFADIGDYIDQPVKTYSSGMALRLAFSVQVHIDPDILIVDEALAVGDAAFQAKAMARI
                     QQILARGTTLLFVGHDLNALRAFCQRAILLEAGRMAMLGSPEEVIEEYLFRVHAKALR
                     DGQPKPIRIAEGFRSEDGGIVAMHLQDGARHLALAHDDLLEVTAHLQLQPGLTDAACI
                     IDILDERGVPLSGRRVRLPDRTQGEECVQLKVAFRVQLAHGVYRIRARLVQAPALHQH
                     HRRVLCRYESDLSFEVVDNSIERFSGLFPLPADITLHTGPADGAEIA"
     gene            complement(20690..22708)
                     /locus_tag="PK399_02065"
     CDS             complement(20690..22708)
                     /locus_tag="PK399_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11945.1"
                     /translation="MKKNILAVALVAGLGLAGAAAAYNYGTSLADDVAAPADSDAALA
                     DAENVSYEMLVASNYNWTMNEALVFDIETADNAAAITDGFTVRVALNQRCTPHNAPAT
                     GIVSPPTNVGVCAPTSDGAAFAFGAPGNILVGATSDIGSPNYDARLVLDPAIAVDWKV
                     TFDGFTSGNTVATFRIEPTSTTPVGPGTVNGIITFKWNNAQLTSLNEFAATAAAGGSA
                     KVDAEFWMVSVSNDSRFVNSTRSRTILESRRGVVACATPNPAEVDKYIDVADNWNEDQ
                     IPKTRFSWDGKLGSATDASTVAAFQLPAGDDYDAQVIDLGDVTLGTGTITGSGTFTYW
                     GAGNAFNGVADVFQTVIEAGGGNNWNAFDNLGVDDDVYLVNGTCESGTIIDQGTVSGT
                     TVTFIYNAQEIESAQAGFINVGGVNRGLTVCGYVDTDTIIDDHNNSVTTTFYRSGMYP
                     ANGSAPGDTATAYPTSGYANTVGSRVLADAACVLLPLRYNGSTMEIFTINPGSNTEQR
                     SFVRLTNRGATAGYVSLEGIDNNGDRGASQVRVWVPAGASVQLNASDLEDGTNGAIGA
                     WGTGAGKWRAVVTAEFPGLVATSLVNTSGTRTLSNITDSDTRGEQYDRDFNEGTFASE
                     VGERPSDHVQEVTPDFHGNGDYTGEPGGPDADDGPAGGQTTPDGNPGL"
     gene            23173..23973
                     /locus_tag="PK399_02070"
     CDS             23173..23973
                     /locus_tag="PK399_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW11946.1"
                     /translation="MLNTLAHPWRHGALIRILAWRDISGRYRGSLLGSFWSLLTPLLM
                     LAVFTFVFGVVAPTRWPGAEEQGIGMFALRLLAGMVVHTLLSEVLSRAPTLVTSQPNY
                     VTKVVFPLEALGWVALLTGIFHAVMALVVLVVLDGAFGTGFSWTMLALPLVLLPYALL
                     LLGLAWLISALGVFLRDLAQLVGPLVMVCMFLGPVFYPREAMPAAAQQWLALNPITVP
                     VEQVRRVLFDAQWPQWDVVAQYSLVALAVYAFGLWAFSRLKKGFADVL"
     gene            23963..25663
                     /locus_tag="PK399_02075"
     CDS             23963..25663
                     /locus_tag="PK399_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPW11947.1"
                     /translation="MSSEAARAAPRDRLLGELLLDAGLLGVADLDRGLALQQKIGGRL
                     GSVLMRIGAVSEDNLLQVLCRQLGLPVMGADVPSPDEDAMRLTALQAPTGIDWLLDQQ
                     VLVWPGEGNDLLCAARDPLLPSLREAMRHAFPGRRVHWCLCRAQDLERMLDGLAHHAR
                     SDEWSGGDEQQLRAMAEEAPVVELVSNLMAQAVEQRASDIHLEPEERTFSVRFRIDGV
                     LHNRLSLPRDRFDAVASRLKLISGMDIAERRLPQDGRLSTRVGGQEMDIRVSALPGVH
                     GESIVMRLLPKERKELGLERLGFEADHLAMMKAWTAEANGIVLVTGPTGSGKSTTLYA
                     SLAAANDGLKKIITVEDPVEFQLPAITQIQAHPDIGLTFASALRSILRQDPDVIMIGE
                     IRDLETAQIAVQAALTGHLVLSTLHTNDAISAFTRLIDMGVEPFLVATPVRAVQAQRL
                     VRRLCPHCARPHAVPAAIEAEVRPFAERVLAGMAPRWMEAVGCSQCMNTGYRGRLGIY
                     EMVPVTEAMQHLIVSGASVNEMKKLARDEGCRFLREDGLLKAWQGQTTVEEVLRVTGS
                     "
     gene            25671..26864
                     /locus_tag="PK399_02080"
     CDS             25671..26864
                     /locus_tag="PK399_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPW11948.1"
                     /translation="MPRYHYHALNAEGISLEGMLRADNERDVARQLERRGLSVVEVRS
                     GEAAGRERRGRLRHGDVILALQELATMLTSGVSIADAVGSQALGAHHPRIVAAFTTMS
                     RDLQRGLAFSATLAKCGLPLPEYVVQLARAGELTGELGRALRDASTQLEYEQQLRNEM
                     RNALIYPAVLVLAGVAAVAIMFIFVVPKFASLLQQAEDLPLLAWVVLAFGTWTREHFL
                     LLLVLLAAAGGGAAWALRKPELRARGMDAMSRWPIVGPWLVEADTARWAKVLGALLGN
                     KVPLMRGLELAQSGLQLPQRRARMGEVTRAVRGGASLADALEDHDALTATGYNLIRVG
                     ERSGKLASMLDSLARLYEEAGRNRMKRVLILLEPIAILVIGSVIGTIILGVILAITSA
                     NDLAV"
     gene            26897..27358
                     /gene="gspG"
                     /locus_tag="PK399_02085"
     CDS             26897..27358
                     /gene="gspG"
                     /locus_tag="PK399_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HPW11949.1"
                     /translation="MQRRIPAVPRQAGFTLLEMIVVLVIIGLIMGLVGPRLFGQADRA
                     KVQTAVTQAKMLRGALQTMRLDISRWPTDQEGLALLTTKPANADAARGWAGPYLDEAV
                     PQDPWGQPYQYSQQPSGESPITLYSFGADGRAGGEGQDADVGYPPSQPATP"
     gene            27372..27758
                     /locus_tag="PK399_02090"
     CDS             27372..27758
                     /locus_tag="PK399_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPW11950.1"
                     /translation="MRQGGFTLLEMIVVLAILGLAAALVGPSMLRGIDSWRRQAAMDG
                     LLDQLRALPGNARASGKPITLSTATLASTTPPLRIDGDWTLSVPEPWSVAANGVCQGG
                     DVVVANAFGERTVRVAAPFCDPTVQP"
     gene            27758..28195
                     /locus_tag="PK399_02095"
     CDS             27758..28195
                     /locus_tag="PK399_02095"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPW11951.1"
                     /translation="MRLRRAPARQHASGFTLLEAIVALVVFSMGAFALYGWLSTNLIT
                     LERIRDRQQVEAAMHSALDIVRRTNPMETPTGQRAVGDLMVSWTSVPMEALRPNTDQS
                     GYPGFYTVGLYDATIRVLRDGRELHVFHVRQIGWKQVRNGAEP"
     gene            28192..28809
                     /locus_tag="PK399_02100"
     CDS             28192..28809
                     /locus_tag="PK399_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPW11952.1"
                     /translation="MNACPARGAAGRSPGFTLMEMLVTLVLISFATMLMFQMLGSYRI
                     ASERVRGQAGHIDRQALFQSWFRDSVHGLYAAPNLHFIGDAAHFSATTLNPLYAPEGS
                     PIAIAWSLRENAQGAAEIVYSEDGRERWQLPLQGGGEVEFTYFDANWRASDAWPPKLG
                     KVNPEALPAVVGLVRRYKDGDRPLLVAVLGPLEPPRRLYGEEELQ"
     gene            28806..29873
                     /locus_tag="PK399_02105"
     CDS             28806..29873
                     /locus_tag="PK399_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HPW11953.1"
                     /translation="MRPVPRARGFVLVLVLAMLVILGLLAGTIAAVTGRLAEQAQQRA
                     QAMQDAVEIASTRATVLYLLSTQRMTLGGLTVDRLVSFGEDGVRPMQSLEDLDSTMPV
                     GSELHLDGSAYQGVGGVRFALQDDYGLFGINWYPHWRLEKLLAQGGHPRPVPTEALIN
                     RLFDYQDRDDLYRLNSMEADGYRKAGMTPPTNLPLATPMEVMRVVGWAQALSFLSPAE
                     ISETITVEAVGGINVNTAPARVLRVIDGVDEEKAARAIAFRKVQPFMTDDAFFTFLGL
                     PKSLDSPVAVYPATSGTLKLWPSHGGQVGLVHWTMTPFEDKGRPWREDYELIQSQAPS
                     QDDVALPVRSRLFGQQVAARK"
     gene            29887..30888
                     /locus_tag="PK399_02110"
     CDS             29887..30888
                     /locus_tag="PK399_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11954.1"
                     /translation="MAEGGNGQEWILARGYCAYTVLDGAAVPPRKRRGFVDMAVSRWS
                     PFADAQSHVEWVGDRAMVWAWSKSRVLAGPEDTLLPSPRRSWPESLFRGQPRAEGEEL
                     VALDEGLEARAWRDGVMTASRWWPEAPDLPEWNEFRRGAGLAPASAMPVPASYPLVDH
                     PWAAPKAIALGEAFGHYRDYLAMAVVGIGAAVMSALLVGVLALKVSNWQLDRDIAERE
                     QALEKIIDARDRAQQGRAAIDARLSLRPPAGQVELLSLVSGLMRGNWQLVEWKMPDAQ
                     TLEVTAKMLNADPGAIVSAWEASKRFTEVTAEIGKQPETVVVKARILRAAPAGGSRK"
     gene            30885..31490
                     /locus_tag="PK399_02115"
     CDS             30885..31490
                     /locus_tag="PK399_02115"
                     /inference="COORDINATES: protein motif:HMM:NF022201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HPW11955.1"
                     /translation="MSAALPAGTRKIREEWRSNPRLRWGAAIAAAIGFLYLCLVLADW
                     RRELHEEYQQRTLQLYKMTELAGQEQWLVRAEGAQAVEKALQAEIPNAATIGLAQAEV
                     QTMARQLINAFGRRLSSDARPPAEVTGQPGLWRVPVTLRGLVSQPQMLEILRRVEDSD
                     RLMVVDEFSFTFVKGVPNMSMTLVAYYRVGAPAQGARNGSR"
     gene            31477..32049
                     /locus_tag="PK399_02120"
     CDS             31477..32049
                     /locus_tag="PK399_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11956.1"
                     /translation="MAVADLLRRHRFLAVAIAGGVVGSVIGLFLPIRAADPPKAEEAA
                     WSLPNAQALKRFRDDQFQSVRRARFWGELAMPGRRNAEQPSSWSLHAIVTRPVVQVGI
                     GANGKPGSSWVRLGGALPDGATLVAVDRDRIWFEKDGCRRMRALYPDKTKSDADACIG
                     AADDGTAPASTPPPRPSASPAAAPVAGKPL"
     gene            32076..>32499
                     /locus_tag="PK399_02125"
     CDS             32076..>32499
                     /locus_tag="PK399_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11957.1"
                     /translation="MNNLRPFLVATGIALLAGACATPRLAVPEPLPSPGSSSQAETGF
                     DQLNGTTKQAPLGLQRSGTPQIPGEGGSLVAGAQGEPMPPLKGGAVNVNIEGMPVPAF
                     INEFFGSILGTGFQMDPQVSRMNDLVTLRTSSPQSPQNF"
BASE COUNT         5046 a        10867 c        11364 g         5222 t
ORIGIN      
        1 ggccacgctg gaaggacgcg cgctgcacaa cgtgcagaac gcgatgttcg cggcgtcgat
       61 cgcgttctcg ctgggcatga agctggatgc gatccgccac ggcctgcgca ccttcgacac
      121 caccttcttc caggcgccgg gccggatgaa cgtgtacggc gaacatccgt tcaaggtgat
      181 catggactac gcgcacaacg cgcacgccac cagcgtgatg gcggacctgg cgcagcgcct
      241 ggacgtgggc ggacggcgca tcgtcgtcgt cgccggcccg ggcgatcgcc gcgacgagga
      301 cctgcgcgac atcgccgcca ccgtggccgg caagttcgac cattacatcg tccgccgcga
      361 cgatggcctg cgaggccgcg atggcgacga agtgccgcgg atcatcgcca gggcgctgga
      421 ggccgcgggc gtggatgcgt ccgcgatcag catgatcccc gacgagcagg aagcggtgga
      481 tgccgccctg cgcatgggcc agccgggcga cctgctggtg gtcttcgccg atgcgctggt
      541 gcgcacctgg aagcagatca tccgcttcaa gcccgagggc gagatgcaga cgtcgaccgc
      601 gcgtgcggaa gtgcccacgc tggaaaccac gctggacgag caactggtcg cggcgatgga
      661 aggcgttgtg cgcgatgagc gcggcctgcg tttcgagcgc gaggaaagcg attgagcacg
      721 gtcgcgatgc aggaaccgtt cgaggattcg cgccggctga ccggggccaa cctgtatttc
      781 gacggatccg gcgcggcgct ggagacggcg catggcttga cgttcgatgc cggcaccccg
      841 caacgctggc gcgcgaacat ccacaaggcg ttggcgttgc tcggttggcc gctcggtgcc
      901 gtggtggtgc gcgagcacgc cagcggcgcc tcgctggcgt tcgaagcgcc gttcgaccag
      961 ttgtacgtcg ccaccgaagt gaacgagtgg gcgctgtatt cggcgatggg cctgcgtgcc
     1021 ggcgatacac ccgtggacga cgaagcggag ctgccacgcc cgcatgtcgc ccatcacgat
     1081 gacgatgaag cattgcgcca gttgcaggcg cttgcggcgg cggaagcaaa gccggccttg
     1141 cgcgcgctgg tcgaagacgc gcatgcgcgc ggcgttcccg cgcatgccga cgacgatgcg
     1201 ctgtcgctcg gcgagggcgc gacatcgcag cgctggccat tggccgggtt gccggcgacc
     1261 gacgacgtgc catgggcgcg actgcatgcg gtccccaagg ccgttgtcac cggttccaac
     1321 ggcaagacca ccaccgtgcg cctgctcgcc gcgatgctgc gcgcgcatgg cttgcatgcc
     1381 ggttacagca gcaccgacgg cctgttcatc gacggcgagc gcgtggaagc cggcgactat
     1441 tccggcccgg tcggcgcacg caccgtgctg cgcgatgcgc gggtcgatgc cgcggtgctg
     1501 gaaaccgcgc gcggcggctt gctgcgacgt ggcctggtcg cccgcgatgc gcgcgtggcg
     1561 gtggtcacca acatcgcggc cgaccacttc ggcgaatacg gtatccacac gctggaccac
     1621 atcgcggcgg tgaagctggt cgtcgcgaaa tcgctcgcgg ccgatggcct gctggtactc
     1681 aatgccgatg atccgatgct ggtcgcgcat gcgccggcat cgcgtcgcat cggctggttc
     1741 gcgctggcat tggccgatgc gatggcgcgc ggcacgcatg cctgcggcgt gcgcgacggc
     1801 cgtctggccc tgtcgcatga cggcatcgaa cacgatctcg gcgaggttgc cgcgatgccg
     1861 ctctcggtgg gcggcgcggc gcgctacaac atcgccaaca tcgccgcggc gtcgctggcc
     1921 gcgcatgcgc tcggcatcgc ggcatcgacg atcgccggcg tgcttgcgcg cttcggtgcc
     1981 cgcaatgccg acaaccccgg ccgcctgcag cgctggacgc tgggcgatgt cgacgtgttg
     2041 ctggactacg cgcacaaccc ggacggcctg cgcggcctgc tcgacgtcgc cgccggcttg
     2101 cgtcgcggcg ggcgcctggg attgctgctg ggccatgccg gcaatcggct ggaatcggat
     2161 ttccatgccc tggcggcggt cgcagcggac gcgaagcccg aacgcgtgtg gctgaaggac
     2221 atcggcggcg actacctgcg cggacgtgcc tccggcgacg tggcgatgat cctgcgcgat
     2281 gccttgctcg catcgggcat ggccgagacc gacctgccgg tctgcctgga cgaggcgcag
     2341 gcctcgcgcg aagcgttggc ctgggcacgg gccggcgacc tgctggtcct gccggtgcac
     2401 gagctggcac ggcgcgacga ggtcgtggcc ttgctggatc acctgcaggc cgagggttgg
     2461 cgcgcgggcg aggtgttgcc ggtcgcatga ggtgtcgctg ttcgtggtga gcctgtcgaa
     2521 ccacgggagg cgtcacgtca gtcggttccg ttcatggttc gacaggctca ccacgtacgg
     2581 acctgcattt cgctgcacca cgaagcgagt gcgccggctc gcttagaatg gcgctccccc
     2641 gcgagcgccc tgccatgcac gacgaccgtt tcgcacgcct gcccgagccg ccctactacg
     2701 cggtgatctt cagttcgcgc cgccgtggcg atgccgccgg tggctatgcc gaggccgcga
     2761 cccgcatgga agagctcgtc gcaacgcagc ccggtttcct cggcgccgaa tccgcgcgcg
     2821 atggcgaagg cttcggcatc accaccgcgt atttcgacag cgaagcctcg atcaaggcct
     2881 ggcgcgagca tgccgaacac gccagcaccc gccagcgtgg ccgcgaggcc tggtattcgc
     2941 agtacgccgt gcgcgttgcc aaggtcgaac gtgcctatgg ctgggagcgc gccgatgcat
     3001 gacgtgctga agcgcatcct ggcgcgcaag caggaagaag tggccgaacg tcgcgagcgc
     3061 gtctcgctgt tcgaactgaa ggcgcgcgcg gcatcggcac cgccggtgcg cgggttcgcc
     3121 gatgcggtgc aggcgaagat cgccgcgggc ctgccggcgg tgatcgccga agtgaagaaa
     3181 gccagtccca gcaagggcgt gatccgtgcc gatttccatc ctgcggacat tgcccgcagt
     3241 tacgaggccg gcggcgcggc ctgcctgtcg gtgctgaccg acatcgattt tttccagggc
     3301 agcgacgcct acctgcagca ggcgcgcgcg gcgtgttcgc tgccggtgct gcgcaaggac
     3361 ttcatcgtcg atgcctacca gttgcacgag gcgcgcgtgc tcggtgccga ctgcgtgctg
     3421 ctgatcgccg ccgcgctgga cgaccggcag ctgtccgaat tcgccttcat cgccgacgag
     3481 ctcggcatgg acgtgctggt cgaggtgcat gacctggacg agctcgagcg ctcgctgccg
     3541 gtgccggcgc gcctgctcgg catcaacaac cgcaacctgc gcaccttcga ggtctcgctg
     3601 caggccacgc tggacctgaa ggcgatggtg ccggcggacc gcgtgctggt gaccgagagc
     3661 gggatcctgg cgccggccga cgtgcagttg atgcgccaag ccgacgtgca tgccttcctg
     3721 gtcggcgagg ccttcatgcg ccagccggat ccgggccgcg cgctcagcga cctgtttgcc
     3781 gcgtgagcgt gcacgaagcc gcatcggcat tcccggtgcc gcgcgacgat gcgccgctgg
     3841 tggtgttcga cttcgaccac acgctgtatg acggcgactc cggcagtcat ctgttcaagt
     3901 ggctgatcca gcgcgcgtgg tggcggcagt tgctggcgat cctggcggca cccgtggccg
     3961 ggccgatggt ggcgttcctg ccgacgcgtc gtgccggcat ttcggtattc gtctggatcg
     4021 gcacgatcgg cctgcatcgg cgtcgcgacc tcgacgcatt gatcgatgca tatgtggccg
     4081 gccatgtcga cgccatccgg tcgcggctgt tgccgatcgc gctcgaggtg ttccgcgcgc
     4141 accgcgaagc cggcgaccgc gtggtcgtgg ccaccggcgc gccgccggag ctcgcacgcg
     4201 cgatcctggc cttcgttgcc catgaagacg tgccggtggt gggcaccctg gtcgggccga
     4261 aattcggtgc ggtcgcggcg atccgccact gccaccacgc gatgaagatg acgatgttgc
     4321 gcgatgccgg cttcacccgg ccggtcgcga tcgcctacac cgacagcagc gccgacctgc
     4381 cgctgttgca ggccgcggtg cgcccggtgg tggtgaatcc gaaggccggc agcgtggcga
     4441 tgttccgcaa ggtgctgcca gcgggcacgc cgatcctcaa ttggggttgc gccgaccgcg
     4501 cgggagatcc ggtggcctga gtcggcaatg cctggcccgg gtcagcgaag cgcgtccggg
     4561 tcaggccggt gcctgggcgg catcctgcac ggtcaggcgc cgagttgctt cacgaaacgc
     4621 acggagctgt ccttgtagcc gcaggcccgg tagaacgcat gcgcctcggt gcgctgcgag
     4681 ccgctggtga gttcgatgcg cgcggcaccg ccactgcggg cacggcgctc ggcttccttc
     4741 agcagctgcc gaccgatgcc caggccctgc gcggtcgggg tgaccaccag cgcggtgacc
     4801 cggcaggtga tggtgcccag cggcaggtag tacatgaaat ccagcgcgac caggccgcag
     4861 accgcaccgt cgcggcgggc cagcaccagc gcctggcggt cgttggcggc gatggcatcg
     4921 atccgctcgg cggcgtcttc gatctcgcag ggatagccca ggtcggtgag gaggcgggcg
     4981 acgtcgtcgg catcggcgat catcgcgctg cgcaggtcgg tgccgagcgg ggaggggccg
     5041 gaggagaacg cctggctcat gcgcgggtca gtcgcgggtg ccgtagacca ccacggtctt
     5101 gccgcgcgcg tgcagcttgc cgtcggcctg cagtttcttc agcacgcggc cggccatctc
     5161 gcgcgagcag ccgaccaggc gcgccagttc ctgccgcgag acgcgcagct gggtgccctg
     5221 cggatggctg agcgcgtcgg gttcgcggga caggtcgaac agggtgcggt agatgcggtc
     5281 gctgacgtcc aggaacgcca ggcgaccggc cttgcggctg gtatccagca ggcgctggct
     5341 gatctgcgcg cccatcgagt agagcaggcg ggcggcatcg ttggcctggg tggtgagcaa
     5401 ttgctgcagg cgctcgtagc cgatctcggc cagttcgcag gcggtgcgcg tgcgcagcgc
     5461 cacgccgcgt tgctgcgctt ccacgaacag gcccatctcg ccgacgaact cgccgggccc
     5521 gatgtaaccc agcaccagct cgcggccgtc atcctcgctg gcgatgatgc tgaccgagcc
     5581 ggacatgatg tagtacaggg tgccggcgtc gtcgccgggg cggaacacgt cggtccgcgc
     5641 cggatacttg cggcggtggc aatgcgcgat gaagcgctcg atggtcgcgg cgtccgcaag
     5701 cagcggattg atcgcgcgcg gcatcacggg cttgctgtag ctggtgctca tcgggacgcc
     5761 tcgggggatg ccccagctta ggcggaaccg tgatggcggg caaacttctg cacgcgccgg
     5821 ccggcgcttt gccgcataat cgcgggcctg ttcgagccat ccggaactgc ctgccgtggt
     5881 caaaccgctg ccgcgcctga agctgcaagg cttcaacaac ctcaccaagt cgctgagctt
     5941 caacatctac gacgtgtgct acgcagcgtc ggaagacgag cgcaggcgct acatcgagta
     6001 catcgacgag gaatacaacg ccgatcgcct gacccagatc ctcacggacg tggcggagat
     6061 catcggcgcc aacatcctca acatcgcccg ccaggactac gatccgcagg gcgcgtcggt
     6121 gacgatcctg atctccgagc agccggtggt cgacaaggcc gatgccaagg gcgtgatctc
     6181 cgatgcggtg gtcgcccacc tggacaagtc gcacatcacc gtgcacacct atccggaaac
     6241 ccatccggac aacggcatcg ccaccttccg cgccgacatc gacgtgtcga cctgcggcgt
     6301 catctcgccg ctgaaggcgc tgaactacct gatcgagtcg ctggagtccg acatcgtggt
     6361 gatggactac cgcgtgcgcg gcttcacccg cgacgtgaag ggccgcaagc actacatcga
     6421 ccacaagatc aattcgatcc aggactacct ggccaagaac gtcggcgccc gctacgagat
     6481 gctggacgtc aacgtctacc aggagaacat cttccacacg aagatgcaca tgaaggaatt
     6541 cgacctcgac aactacctgt tcgaggagaa ggcgaagaac ctgtcgttca aggagcgcat
     6601 gaagatcgag gcgcgcctga agcgcgagat cgaagagctc taccacggcc gcaacctggt
     6661 cgactgaccg gtcgcgacgt gatgacgaga aagccggcgc aagccggctt tttcatgcgc
     6721 gggactcagc ctgcgcccgg tcgcgccggc aggcccaagg cgccagcgac cagttgcatc
     6781 gcctccatgt cgcgctcgcg ggtgatccag ccggccagcg tgggttcgcc ggtttcgacc
     6841 gtccacaggc agaagtggtg ttcgcgcaac tgcacgaagg cggcgtgcag caacgcggac
     6901 gtgtcgggtg cttcgcccgc gtcctcgtcg agcggccagt cgatccgcag ccgccagcgc
     6961 gccgccagct cgtccagcgc ctccatcagg ccctgcgcat cgtcctcgcc cacgtggaag
     7021 ccggccttcc agtcggtcgc gtcgcgcagc agcagggccg ggtcggcgcg ttcgccggcc
     7081 tcgtccaggg cgcgctggaa ggcggcgaac tgctccagcg cggcatcctc gtcatctgga
     7141 ttgaccagca ggaggaattg ccaggccacc gcctccagcg cgtcctcttc ctccacgccg
     7201 gcctgcgcgt ggaaggcgtg ctcgaagttg tcgtcggggt cgtattcgtg ggcggccatg
     7261 gtcgcgtcct gcggttgcgc ggcatgcgca agcgcgcagc atacgtccgc cgcgcgcacg
     7321 gggcggctgc ccgtagaatg gcgcaccccc gaccaggccc cgcggccacg gacgcgccat
     7381 ggccgccaac gccacgatct acaaggccga gctgcaggtc accgatctcg accgccacta
     7441 ctacgccagc catgcgctga ccctggccca gcacccctcg gagaccgaga cccggctgct
     7501 ggtccggttg atcgcgttcg cgctgcatgc agacgagcgc ctggagttcg ggcgcgggct
     7561 gagcgacgag gacgaaccgg cgttgtggcg gcgcgactac accggcgaca tcgagcaatg
     7621 gatcgagctg ggccagccgg acgagggccg cctgcgcaag gccagcggcc gcgcggcgca
     7681 ggtggtcgtg gtcggctacg gcgggcaggc cgccgaggcg tggtggaagc ggaacgccgc
     7741 ggcgctgggc cggctgcgca acctgacggt gctcgagctg gacgatgccg aagtcgccgc
     7801 ggcgacggac ctgctcgggc gcggcatgcg ggtcaccgcg atgatccagg acggcgagct
     7861 gcagttgatg gatgccgaac gcagcgtgtc gttgcggccg cggatccgcc tgcagcatgg
     7921 ggtggccacg ccggactgag ccggctcaaa gccggtaggc gacggccttc atcagcgtcg
     7981 atgccgcctt catgagggcc ggcaccggct gcggcaacac cttggcgccg gcctgctcgg
     8041 cctggtcggc gtggcgggcc tcgtcgtcct tcatcacctg caggatcgcc cggctgcgca
     8101 ggtcggcgtc gggcagggtg tgcaggtgtt cttccaggtg cgcctcgacc tggtgctcgg
     8161 tctcgaccac gaagcccagg ttccagccat cgccgcgcag ccccgcgagc gcgccgatcg
     8221 cgtagctgcc ggcgtaccag accgggttga gcaggctggg tcggctgtcc agctcgcgca
     8281 gccggtcggc gcaccaggcc aggtggtcgg tttcttcctg cgctgcctcc agcaggtggg
     8341 cgcgggtggc cggatcgcgg gcaaccgcgg cctggccgat gtacagcgcc tgcgcgcaca
     8401 cctcgccgac atggttgatg cgcatcaggc cggcggcgtg gcggcgctcg gcatcgtcca
     8461 ggaccacgtc gggagtggat tcgccggggt tggcgcgccc cgcgcgcgga ctgccggcca
     8521 cggtggacag cgcgttctgg gccgaatcca gcaggcggtc taggcgactc aggatgcgtg
     8581 aggtcgacat gccgccattc taggcggccg gaagggcggg ttgcacgggc cggcgtcgcg
     8641 ggctatcatc ccgctccttt cgcgccgaaa tcccgtcaag ggccgcccgt ctcgggtcgg
     8701 atcgaaagaa caacgcgagg cgaccttccg cagccgcaac ccgctgcgca accagcccga
     8761 ccatgcaccc ctgaagagtt cccatgaaga ctttcattgc caagaacgag accgtccagc
     8821 gcgactggta cgtcgtggac gccgagggca agaccctggg tcgtctggcc gcagcactgg
     8881 cctaccgcct gcgcggcaag cacaagcccg ttttcacccc gcacgtcgat accggcgatt
     8941 acctcgtcgt gatcaacgcc gagaagatcg cggtgaccgg caagaagctg accgacaaga
     9001 tgtatcaccg gttcaccggt tacgtcggca acctgaagac cgaatccctg cagcaggcgc
     9061 tggagcgcca cccggagcgc gtgatcgaga tcgcggtcaa gggcatgctg ccgaagaaca
     9121 ccctgggccg cgcgatgtac cgcaagctca aggtctacaa gggctccgaa cacccgcacg
     9181 ctgctcagca gccgcagacg ctggacatct gatcatggcg atttcccaga attacggcac
     9241 cggccgccgc aagtcctccg ttgcccgcgt gttcctgcgc aagggcaccg gcaccatcac
     9301 cgtcaacgag cgcccgctgg acgagttctt cggccgcgag accgcgcgca tgatcgtgcg
     9361 ccagccgttg gagctgacca agcacatcga gaccttcgac atcgtcgcca ccaccaccgg
     9421 cggcggcacc accggccagg ccggtgcgat ccgcctgggc atcgcccgcg cgctggtcga
     9481 gtacgacgaa accctgaaga tcgagctgcg caaggccggt ttcatgaccc gcgatgcgcg
     9541 cgaagtcgag cgcaagaagg tcggcctgca caaggcacgt cgcgccaccc agttctccaa
     9601 gcgctaatcc gagtcgcccg ctggcgttgt cgtggcggct tgcctgtatt gtccgtacag
     9661 gcggctccac cactcctagc cagccaagga tcggatacgc gcttgctggc gcggtttttc
     9721 cagcaagcgc gtttggttct acaattcgct ccatagcccc gtcgccaagc ggtaaggcac
     9781 ctgactctga ctcaggcatt cggaggttcg aatccttccg gggctgccac attcgcaaca
     9841 ccgtcgaaac ccgccgcaag gcgggtttcg cgtttgcgcg atgccattcc ggaatgcgtg
     9901 cgggcgtggc gcgatgcgac aatgcgcgtc catccccgga gccgcgcatg gtcgcccact
     9961 tccccgcccc gagcgtgccg ctggacgacc tggaccgtgc ccttcgggac gatggctatg
    10021 ccgtgctgga accggcatcg gtggttgcgc tggccggggt tggcgctggc ggtttcgatg
    10081 gcttgctgcc tagttgggac gacctgccgc aggacgacta cctcaaggac ggcggccgct
    10141 accgcaagcg ccggcatgct tcgttcatcg ccgaagcggg cgcactggtg caggtcccgc
    10201 ctcgccgcca ttggcagccg gtcgagtaca acgccctgca tggcgggatg gagcgctggt
    10261 tcgcgccggt gacgcccgag gtgatggcgc atccggcctg gtcgaagctg gtgctggcca
    10321 tcgccgatgc gtgcagtcgt tgcaagcctg cgccgcgctg gcacgtggag gcccaccagt
    10381 tccgcatcga caccaccgac ggcctcggcc ggcccacgcc ggaaggcgcg catcgcgacg
    10441 gcgtcgattt cgtggcggtg ttgctggtgt cgcgcagcgg catcaagggg ggcgaaaccc
    10501 gggtcttcga cgccaacggg ccacgcggtc agcgcttcac cctggatgcg ccgtggtcga
    10561 tgctgttgat ggacgacgcg cgggtgatcc acgaatccac gccgatccag ccgatcggca
    10621 cgttcggcca tcgcgacacg ctggtgctga cctatcgcgc cgctgcgttc ctgggcgacg
    10681 actgacggga ttccgctcgt ggtgagcctg tcgaaccatg ggtggaatgc tggccggccg
    10741 gccgttcgtg gttcgacagg ctcaccacga acggcaggtg gattgcatct tgcgcttgcg
    10801 tacgcgtcag cttccacatc gttacaattg caaccatctc tccgaaacag tctgccgagg
    10861 tcgcgatgaa actgggttct ctgaaggaag gcggccgcga cggcaccctg gtcgtggtgt
    10921 cgcgcgacct gaccaaggcg gtgcgtgcca ccggcatcgc gcccacgctg cagcgcgcgc
    10981 tggaggactg gagcaacacc gcgccgcgcc tcaacgcgct ggccgagtcg ctggacgcgg
    11041 gcgatgccga cggcgtgttc gacctcgaca tgcaggcgct ggccgcgccg ctgccgcgtg
    11101 cctatgagtt cgtcgacggc agcgcctacc tgccgcacgt cgcccgcgtc cgcaaggcgc
    11161 gcggcgccga ggtgccggag agcttctacg tcgatccgct gatgtaccag ggcgtgagcg
    11221 ccggcttcta cggcccgcgc gatgcggtga aggtggtcag cgaggactac ggcatcgacc
    11281 tcgaggccga gatcgtgatc gtcaccgacg acgtgccgat ggcggtcacg ccggaacagg
    11341 cggcatcgca tatccagctc atcggcctgg tcaacgatgt gtcgctgcgc aacctgatcc
    11401 cgaatgagct ggccaagggc ttcggcttcc tgcagagcaa gccgcggagc gcgctgagcc
    11461 cggtgttcgt gacgccggac gagttgggcg ctgcctggcg cgacaacaag ctgcacctgc
    11521 cgctggtgac ccatatcaac ggcacgtggt tcggcgcacc ggaagccggc gtggacatgc
    11581 agttcgactt cgcccaactg gtggcgcatg cggcgaagac gcggccgttg tcggccggca
    11641 cgatcgtcgg ctccggcacg gtggcgaacg aggacacctc gctgggcgcc tcctgcttcg
    11701 ccgagcagcg cacggtggaa accttgcgcg acggcaagcc gtccacgccg ttcatggcgt
    11761 tcggcgacct ggtccggatc gaggtgctgg acgccgccgg caacagcgtg ttcggcgcga
    11821 tcgaacagcg gatcgagcgc caggacatcg cctgagccac cggcgggacg ggcggtcgcc
    11881 gcaggggcgt gaacggcatg ccgcggcacc ggtcgggtcg ctaatgtgcg ttgttgccca
    11941 ttgggcgggg acccgatgac cgaaacgctg cagctgtatt cgtactggcg ttccagcgcg
    12001 gcctatcgcg tgcgcatcgg cctgaacctg aaggggctgg cgcacagcat cgtgccggtg
    12061 catctgctgc gcgatggcgg gcagcagcat tctgcgggct accgcgagat caacccgcag
    12121 gaactggtgc cgacgctctg ccatggccag cgccgcctcg gccagtcgct ggcgatcctc
    12181 gaatacctgg acgaattctg gccggaaccg ccgctgttgc cggccaatgc ccgcgcgcgc
    12241 caccgcgcac gggcgctggc gcagctggtc gcctgcgacg tacacccgct gaacaacctg
    12301 cgggtgctgc agtacttcga gcaggaatgg aacgtgccgc agcccgagcg cgacgaatgg
    12361 gttcggcact ggatcgtcga aggcttccgc gcggcggagg cgatgctggc cgaccatccg
    12421 tcgaccggga tcttctgcga gggcgacatc cccggcctgg ccgactgctg cctggtgccg
    12481 caggtctaca acgcgcgccg cttcggcgtg gacatggcgg cgtatccgac catcgagcgg
    12541 atcgagtccg cttgcctggc attgccggcc ttcgatgccg cgcggccgga acgccagccg
    12601 gacgcgcccg cgccaacctg aacggcaaaa cggggacgga ggtagtcaag cgcgcttgac
    12661 tacctccgtc cccgtcttgg cacccggctc aagcagtggt gtcagtgcga tccgctgccg
    12721 aacacgtcgg cgtaggggtc gtgctcggcg ccgccgcctt cggagaggcg gaacttcagg
    12781 gccaggccat cgcgcgagtc ggcggccttg agcgcctctt ccatctcgat gcggccttcc
    12841 ttgtagagcc ggaacaggca ctggtcgaag ctctgcatgc cttcctgcag ggattcttcc
    12901 atcgccatct tgatctcgtg cacctgcccg cggcggagca ggtcgcggat catcggcgtg
    12961 ttgatcagga cctcggtggc cggcatgcgg cggccatcga cgccgaccac caggcgctgg
    13021 ctgaccaccg ccttgaggtt cagcgccagg ttcatcagca cgttcttgtg cgcgccctcg
    13081 gggaagaaat tgaggatgcg ctcgatggtc tggtcggcgt tgttggagtg cagggtggcc
    13141 aggcagatgt ggccggtctc ggcgaaggcg atcgccgctt ccatcgtcgc cgcgtcgcgg
    13201 atttcgccga tcaggatcac gtccggcgcc tcgcgcatcg cgttcttcag cgcggcgtgg
    13261 aaggtgtgcg tgtccagccc gacctcgcgc tggttgacga tcgacatctt gtggcgatgc
    13321 aggtactcga tcgggtcctc gatggtgagg atgtgcccgg tgctggtgct gttgcggtgg
    13381 tcgatcatcg acgccagcgt ggtcgacttg ccggagccgg tggagccgac gatcagcacc
    13441 agcccgcgcc gggccatgat gatgtccttc agcacctgcg gcagctgcag ttcctcgatg
    13501 ctcgggatgt cgctgcggat cgcgcggatc accatgccga cttcgccgcg ctgcatgaac
    13561 acgttgacgc ggaagcgacc ggagccggcg acggcgatcg ccatgttcag ctccagctcg
    13621 cgttcgaact cggcgatctg cgcctcgttc atcagcgaat aggcgacttt cttcgacatc
    13681 ccggtcggca gcggtgcgct acccagcggc agcaggtcgc cctcgacctt gatgttcacc
    13741 ggcgccccgg acgtgaggaa catgtccgag gcgttctttt ccgccatcag cttgaggaaa
    13801 tagccgatat ccatgctgtc tgccccttgg cgcggccccg gcgattgcac tgccggcagc
    13861 ggcttcccac aatgccgtgg acatacagga cgccacgact ccctatgcaa actagcttcg
    13921 cacacttccg ccgggcgctg caggcccccc ttgccggcct ggtcctggtg atcgcgggct
    13981 gcgccaccct gccaccaccc actgccgaac tggatgccgc gcagcaggcc gtggcccgcg
    14041 cggaggcggc cgatgccgag cagtacgccg cggacgagct ggcgcgcgcc cgcgacgcct
    14101 tgtcgcgcgc ccaggccgcg atggcccgcg ggcgcgacgc cgaagcgcgc gaggcggcgc
    14161 tggcggcggg tgccgatgcg gacctggcgc gcgtgcgcag cagcgcggag accacccgcg
    14221 ccgaattccg ccagcagcag gaaggcatct ctggcttgcg agcgcgcctg cagatggacg
    14281 cagcgccgat gcaggcgctg ccgttcgggg aaccgatggc cggcgcaccg ccggcgcagc
    14341 gcctgcaggc gctggacgcc gatccacggc tcaacccgtt cgcgcagtac gaacgcctgc
    14401 aggcgcgcca ggcgctggcc gcggtggaca ccgccagccg cagcaagcag ccggcggcgc
    14461 tggccctggc cgaccggcgt gtcgccatcg ccgagcaggc cgcgcgcatc gaagccaccc
    14521 gccgtgagat cgaccgcctc gagcgcgagc gcagcgaatt gctggtcgag gccagccgcc
    14581 gcgatgccga acgtgcgcgc gccgaagccg aacgcctgcg catcgaggcc caggtccagg
    14641 ccgaggaagc cgcgcgcctg cgtgcgcagg ccgagcaggc cgaagcggtg ctcgacagcg
    14701 cgcagatcga ccagggcgcg aaggtcgccg cagcccgcga gaaggaagct gcgctggccc
    14761 gccaggaggc cgaactggtg gcgggtgcga aattgccgcc gtcccgccgc gatgcgcgcg
    14821 gcgacgtctt caccctggcc ggcgatgcct tcgcctcggg gcaggcgcaa ctgaccgcgg
    14881 gcgccgccgc cagcatcaag gcattgggta tctatctggc tgcagtgccg gggggcgcgg
    14941 tgcaggtgct cggccacacc gacagccagg gcgaggaggc cgccaaccag gcgctctccg
    15001 agcgtcgcgc ccagcaggtc cgggccaccc tggtcgcggc tggactggcg cgtgggcggg
    15061 tgcaagcgca gggcgtcggc gaggcccagc cggtggccga caacaccacc gccgcggggc
    15121 gggccaagaa ccgccgggtc gaggtcgtgg tggcggacaa tccctgaaaa aacaaagttt
    15181 taacgcaatt tctacctgct gcgaagatcc tcctgaccgt tcatcgtccg ggcttgcggg
    15241 gccgcaggcg caggcgtagt gtcgattccc cggagggttc gaattcgcgg atcctccggc
    15301 cggagaccgg ccgatgcatg tcccgttcga aaacccggag accgccgccg caagccagga
    15361 tcgcggcggc gcaaccgagc gaatcggtcg cgccgttcca cccgttctcg tgcccctcgt
    15421 caacgccccg tgctgcttgc agccggggcg ttcgcatttg cggggtcggc atcccgtacc
    15481 cgtgtccccg tggctcgctc aagccgggcc gcatcctcgg caggtcgatg acctgccacc
    15541 acccgcgccg tcccggccag cctggccggg ctccgcatga gacccaagga ggtttccatg
    15601 ttcgaaggac aaccgcagac ggaactcgaa gcgaagatga aagccagtcc cgagttccgg
    15661 cagctgtatt accggcacca ggaactggac aagcaggtgc tggacgccga gctcggcgta
    15721 ttgcccatca atgacatgac cctgggccag atgaagcgcg agaagctcgc cgccaaggat
    15781 cggctgttgc ggatgtacga cacccactga cgccatgcgc ccacgcgggc ggtgtgcggc
    15841 ctccttgccg gccgccgccg cccgtcccac ccggatcggt ggatgcaggg gccggaaggg
    15901 gcggctgcta gaatcgacgg cttgccgcaa cacgctgacg accgcatggc cacccacgat
    15961 tccgtactcg aactggtcgg cgacaccccg atcatccgtg cccgccacct cgacgtcggc
    16021 ccgtgcacgc tttacctgaa gctggaaagc cagaatcccg gcggctcgat caaggaccgg
    16081 atcggcctgt cgatgatcga ggcggcggag aagcgcggcg acatccagcc gggcggcacg
    16141 ctggtcgaag ggactgccgg caataccggc atcggcctgg ccctggccgc gcagcagaag
    16201 ggctacaagc tggtcctggt cgtgcccgac aagatgtcgc gcgagaagat cttcaacctc
    16261 aaggcgatgg gcgcgcaggt cgtgctgacc cgcagcgacg tggccaaggg ccatcccgat
    16321 tactaccagg acatggccga gcgcatcgcg cgcgagaccc cgggtgccta tttcatcaac
    16381 cagttcggca atccggacaa cccggccgcg cacgaattcg gcaccggccc tgaaatcctg
    16441 gcgcagatgg ccgacgtggg cgggctggat gcgatcgtgt tcggctgcgg cagcagcggc
    16501 accatgaccg ggctgtcgcg ctgcttcgcc acgcactcgc ctcacaccga gctggtgctg
    16561 gcggatccgg tcggttcgat cctggaggaa tacatcaacc gcggcacgct gagcgagaag
    16621 tcggcgagct ggatggtgga aggcatcggc gaggatttcc tgccggcgat cgccgacttc
    16681 agccgcgttg cgaaggcgta tgcgatcacc gacaaggaaa gcttcctgac cgcgcgcgag
    16741 ttgctggcga aggagggcat cctcggcggt tcgtccagcg gcacgttgct ggctgccgcg
    16801 ctgcgctatt gccgcgagca gacctcgccg aagaacgtgc tggtgttcgt ctgcgacacc
    16861 ggcaacaagt acctgtcgaa gatgtacaac gacttctgga tgctggacaa cggcttcctg
    16921 gagcgcgagc agcacggcga cctgcgcgac ctgatcctgc gcccgttcgc cgcgcgcgac
    16981 accgtggtcg tcggcccgaa cgacctgctg atcaccgcct ggcagcggat gaagctgtac
    17041 gacgtcagcc agttgccggt gatggagggc gaccgcatcg tcggcatcgt cgacgagtcc
    17101 gacgtgctcc tgcacgtgta tggcgacgag gcgcgcttcc gcgacccggt cgccaccgcg
    17161 atggtcagca agctggacaa ggtcaacgtg ggggcgccga tcgaggcgct gctgccggtc
    17221 ttcgaccgtg gccacgtggc gatcgtgatg gagggcgagc gcttcatcgg cctgatcacc
    17281 cgcatcgacc tgctcaacta cctgcgccgc cgcgtgcagt aacccggacg gacccgccac
    17341 atgagcgaca acaacgacca cggcctgggc acactggcca tccacggcgg gcaatcgccc
    17401 gacccgacca ccggcgcggt gatgcagccg atctacgcca ccagcaccta cgcgcagtcc
    17461 agccccggcg tgcaccaggg cttcgaatac tcgcgcacgc acaacccgac gcgcttcgcc
    17521 tgggagcgcg gcgtggcctc gctggagggc ggcagccgcg gcttcgcctt cgcctccggg
    17581 ctggcggcca cctcgaccct gctggagttg ctcgacagcg gcgaacacgt catcgcgatg
    17641 gatgatctct acggcggcag ctaccggctg ttcgaacgcg tgcgcacgcg cagcgccggc
    17701 ctgtcgttct cgttcgtcga cctgaccgat ccggctgcgt tcgaggccgc gatcaccgcg
    17761 aagaccaagc tggtctggat cgaaaccccg accaacccct tgctgaagat cgtcgatatc
    17821 gccgcgatcg ccgccatcgc caagcggcat ggcctgctgg tcgcggtcga caacaccttc
    17881 gcctcgccga tgctgcagcg cccgctggaa cacggcgccg acatcgtcat gcactcggcg
    17941 accaagtacc tcaacggcca ctccgacatg gtcggcggca tgctggtggt cggcgccgac
    18001 gcggagctcg gtgagcagct cgccttcctg cagaactcgg tgggtgcggt gcaggggccg
    18061 ttcgacagtt tcctggccct gcgcggcctg aagacgctgc acctgcgcat gcgcgcgcac
    18121 tgcgcgaatg cgcaggcgct ggcggagtgg ctgcagggcc atgccggcgt ggtcgacgtg
    18181 atctatcccg ggctggcctc gcatccgcac catgacttgg cgaagcggca gatggacggc
    18241 ttcggcggca tcgtctcggt gcggatcaag ggcggcttcg agggtgcgaa gcgcttctgc
    18301 gagcgcctcg acctgttcac cctggccgaa tcgctgggtg gggtggagtc gctggccaac
    18361 catccggcgg tgatgaccca tgcctcgatc ccgccggccc gccgcgcgga gctggggatc
    18421 agcgacgacc tggtgcgcct aagcgtgggt gtcgaggagc tggccgacct gcagcgcgac
    18481 ctggagcatg cgctgggctg agcctcagtc gaaggcgatg ccgtggcgtt cgcgcatggc
    18541 ccgccgttcc cgccgcatca gccagcgcag gaaccgcgac tcgaatgccg agggtgatgc
    18601 cggcgggtag tcgccgacga agaccggcgc gtacccggtc cccggtggtg ggggtgacac
    18661 gcccaggaag gtacaggcct cgcgcacgca ggcggccggg tcgttgcgca gccgttcgct
    18721 gcgcagcagc agcacctgcc cacgcggaaa gtgggcgtac agcacgtcca gctggcgggc
    18781 gtagtcgccg cgtgcgcggt aactgaagcg gcgcagcctg gagccacgcg ccaggtcgtc
    18841 ggcatggccg cgcacgcgcc agggttccag ccacgccgcc agccagagcg gcagcgattc
    18901 gtggccacgc tggcgctcca tgtggtggtg cgagcgggcg cgctccaccg ggtcgcgcag
    18961 cagcacgatc cagcgcatcg ccgggttgta gcgcgcgatc cgtgccacgc aacgcgggtg
    19021 gaacaggtag aacggcgtgg cgtcgcccca acgcacatcc ccaggcgcgt ccgggaactg
    19081 ctgcgcgagg cgggcgtcga tgtcgccggt ggaccaggcg tcgtcgaagt ccggtgcatc
    19141 gaacacgtgc gcttccttgt ccgccggcag gcgcaggtcg gggcaggcgc gcaggtactg
    19201 ggcgagcgcc gaggtcccgg ccttctgcgc gccgccgacc aggaacgcga tccgcggctc
    19261 aggcgatctc ggcgccatcc gcggggccgg tgtgcagggt aatgtcggcg ggcaacggga
    19321 acaggccgct gaagcgttcg atcgagttgt ccacgacctc gaacgacagg tcggattcgt
    19381 agcggcacag gacccggcgg tggtgctggt gcagggccgg tgcctgcacc aggcgtgcgc
    19441 ggatccggta gacgccgtgc gccagttgca cccggaacgc gaccttcagc tggacgcatt
    19501 cctcgccttg cgtgcgatcg ggcaggcgca cgcgacgccc gctcagcggc acgccgcgtt
    19561 cgtcgaggat gtcgatgatg caggcggcat cggtgaggcc gggctgcagt tgcaggtggg
    19621 cggtcacttc cagcaggtca tcgtgggcca gggccaggtg gcgcgcgcca tcctgcaggt
    19681 gcatggccac gatcccgccg tcctcgctgc ggaacccctc ggcgatccgg atcggcttgg
    19741 gctgcccgtc gcgcaaggcc ttggcgtgca cccggaacag gtattcctcg atcacttcct
    19801 ccgggctgcc gagcatcgcc atgcgcccgg cttccagcag gatcgcgcgc tggcagaacg
    19861 cgcgcagggc gttgaggtcg tggccgacga acagcagggt ggtgccgcgc gccaggatct
    19921 gctggatgcg cgccatcgcc ttcgcctgga aggcggcatc gccgaccgcc agcgcctcgt
    19981 ccacgatcag gatgtcgggg tcgatgtgga cctgcaccga gaacgccagg cgcagcgcca
    20041 tgccggagct gtaggtcttg accggctggt cgatgtaatc gccaatgtcg gcgaacgcga
    20101 tgatgtcgcc gagcagggca tcgatgcggg ccgtggccag gcccaggatc gcgccgttga
    20161 tgtagatgtt ctcgcgtccg gtcagttccg ggttgaagcc ggaacccagt tcaagcaacg
    20221 cagcgacgcg gccatgcgtt tccacccggc cggaggtggg cggcaacacg ccggcgatga
    20281 tctgcagcag ggtgctcttg ccgctgccgt tcagtccgat gatgcccagc gcttcgccgc
    20341 gatgcagttc gaaatcgatg ccgtgcagcg cctcgtgcac gtgcatccgc cggtgcgcgg
    20401 cggcatgcag cctgtcggag agccggggcg cggcctgcgc cagcaggttg ccggcgcgat
    20461 gcagtgccgg tgcgatcaag cgctgggccg gcgtcgccca cgtgcggaag gtcttgcaca
    20521 cgtcccgcac caggatgacg ggcgcggagc cggacgttga cgcagctgct gcaggcattg
    20581 gggtggcttg gcggatgcgg cccgcattgt aactgcgatc cccacgcgca aaaaaaaacg
    20641 ggcggcccga aggccgcccg tcagtacaac cgtattgcaa tgcagcctat tacaggcccg
    20701 ggttgccgtc cggcgtggtc tgaccaccgg cgggaccgtc gtctgcatcc ggaccgcccg
    20761 gctcaccggt gtaatcaccg ttgccgtgga aatccggggt cacttcctgc acgtgatccg
    20821 acggacgctc gcccacttcg ctggcgaagg tgccttcgtt gaagtcacga tcgtactgct
    20881 caccgcgggt gtcgctgtcg gtgatgttgc tcagggtgcg cgtgcccgag gtgttgacca
    20941 ggctggtggc gaccaggccc gggaactcgg cggtcacgac cgcacgccac ttgccagcgc
    21001 cggtgcccca ggcgccgatc gcgccgttgg tgccgtcttc caggtcggac gcattcagct
    21061 gcaccgaagc gccggccggg acccacacgc gaacctgcga agcgccgcgg tcaccgttgt
    21121 tgtcgatgcc ttccagcgag acgtaaccgg cggtcgcgcc gcggttggtc aggcggacga
    21181 agctgcgctg ctcggtgttg ctgcccgggt tgatggtgaa gatttccatc gtcgagccgt
    21241 tgtagcgcag cggcagcagg acgcaagccg cgtcagccag gacgcgcgaa cccacggtgt
    21301 tggcatagcc gctggtcggg taggcagtgg ccgtgtcgcc cggcgcggag ccgttggccg
    21361 ggtacatgcc cgagcggtag aacgtggtgg tgaccgagtt gttgtggtcg tcgatgatgg
    21421 tgtcggtatc gacgtagccg caaacggtca ggccgcggtt gacgccgccg acgttgatga
    21481 agccggcctg ggcactttcg atctcttgcg cgttgtagat gaaggtcacg gtcgtgccgc
    21541 tcaccgtacc ctggtcgatg atcgtgccgc tctcgcaggt gccgttgacc agatacacgt
    21601 catcgtcgac gcccaggttg tcgaatgcgt tccagttgtt gccgccgccg gcctcgatca
    21661 cggtctggaa cacgtcggcc acgccgttga aggcgttgcc cgcaccccag tacgtgaagg
    21721 tgccggaacc ggtgatggta ccggtgccca gggtcacgtc acccaggtca atgacctgtg
    21781 cgtcgtagtc gtcgcccgcg ggcagctgga acgcagccac ggtgctggcg tcggtggccg
    21841 aacccagctt gccgtcccag ctgaagcggg tcttcgggat ctgatcttcg ttccagttgt
    21901 cggcaacgtc gatgtacttg tccacttccg ccgggttcgg agtcgcgcag gcaaccacgc
    21961 cacggcggct ttcgaggatg gtgcgcgaac gcgtcgagtt gacgaagcgg gagtcgttgg
    22021 aaacgctcac catccagaac tcggcatcga ccttggccga accacccgcg gccgcggtgg
    22081 ccgcgaactc attcaggctg gtcagctgcg cgttgttcca cttgaaggtg atgatgccat
    22141 tgacggtgcc cgggccaacc ggagtggtcg aggtcggctc gatacggaac gtggcaacgg
    22201 tgttgccgct ggtgaagccg tcgaaggtga ccttccagtc cacagcaatg gccggatcaa
    22261 gcaccaagcg ggcgtcgtaa ttcggagaac cgatgtcgct ggtggcacct acaaggatgt
    22321 taccgggagc accgaaagcg aacgcggcac cgtcgctggt cggagcgcag acgcccacgt
    22381 tggtcggcgg gctgacgatg ccggtggccg gcgcattgtg cggagtgcag cgctggttca
    22441 aggcgacgcg gacggtaaag ccatcggtga tggcggcggc gttgtcggcg gtttcgatgt
    22501 cgaaaaccaa cgcttcattc atggtccagt tgtagttgct ggccacgagc atctcgtacg
    22561 agacgttctc ggcatcggcc agtgctgcat ccgagtccgc gggcgcggcc acgtcatcgg
    22621 ccaggctggt gccgtagttg taggccgcag cggcgccggc cagacccaag ccggccacga
    22681 gggcaacggc gaggatgttc ttcttcatgc tagacatgtc taactaactc ctgtcagttg
    22741 gtattgcggt ttgattgcaa ctctttccgg ctgcacggcc catgcccggt accaggaggc
    22801 tgggcctccg gcatccggtt tcttccaggc ttgcatcgat gttctgattg acgttgatcg
    22861 cgcccggctt gcggccatgc gcgactcccc ttcccctggt tgatcaagtt gcagcgcgga
    22921 cgctaaccca cggttcagga atcgtcaatc ctttaaatgt gaataggttg cgtgcctgag
    22981 cgaaaccagc gtcacaacat gacgttgcga catgaatgtg accttttgcg tcagtgttgc
    23041 ccctgttcag cttgtggtgg ccgaggattg gcacggaacc gctagacttc cgtgccggca
    23101 ggacggccgg gatccccgct ccagtccgcc cgtcctgccg tactgaccgc tgatccggag
    23161 cccgcgcccc ccgtgctgaa taccctcgca catccgtggc ggcatggcgc gttgatccgc
    23221 atcctggcct ggcgggacat cagcggccgg tatcgcggtt ccctcttggg ctccttctgg
    23281 tcgctgctga ccccgctgct gatgctggcg gtcttcacct tcgtgttcgg ggtggtggcg
    23341 ccgacccgct ggcccggggc ggaagagcag ggcatcggca tgttcgccct gcggctcctc
    23401 gcgggaatgg tggtgcacac cctgttgtcc gaagtgctgt cgcgggcgcc gaccctggtc
    23461 acctcccagc ccaactacgt gaccaaggtg gtgttcccgc tggaggcgct gggctgggtg
    23521 gcgctgctga ccgggatctt ccatgcggtg atggcgctgg tggtgctggt ggtcctcgac
    23581 ggtgccttcg ggaccgggtt ctcgtggacg atgctcgccc tcccgctggt cctgctgccg
    23641 tacgcgctgc tgttgctcgg actggcctgg ctgatctcgg cgctgggggt gttcctgcgc
    23701 gacctggccc agctggtcgg gccgctggtg atggtgtgca tgttcctggg gccggtgttc
    23761 tacccgcgcg aggcaatgcc ggccgcggcc cagcaatggc tggcgctgaa cccgatcacg
    23821 gtgccggtgg agcaggtgcg gcgagtgctg ttcgatgccc agtggccaca gtgggacgtg
    23881 gtggcgcagt attcattggt cgccctggcc gtgtatgcgt tcgggttgtg ggcgttctcc
    23941 agactcaaga aaggattcgc cgatgtcctc tgaagccgcg cgcgccgcgc cgcgcgaccg
    24001 cctgctgggc gagttgctgc tcgacgccgg cctgctgggc gtggccgacc tggatcgcgg
    24061 gctggccctg cagcagaaga tcggcgggcg cctgggcagc gtgctgatgc ggatcggcgc
    24121 ggtcagcgag gacaacctgc tgcaggtcct gtgccgccag ctgggcttgc cggtgatggg
    24181 tgcggacgtg ccgtcgccgg acgaggacgc catgcgcctc accgccctgc aggcaccgac
    24241 cgggatcgac tggctgctcg accagcaggt gctggtctgg ccgggcgagg gcaatgacct
    24301 gttgtgcgcc gcgcgcgacc cgctgttgcc ctcactgcgc gaggcgatgc ggcatgcctt
    24361 ccccggccgc cgggtgcact ggtgcctgtg ccgggcgcag gacctggagc ggatgctgga
    24421 tggcctggcc caccacgccc gttccgacga gtggagcgga ggcgacgaac agcagttgcg
    24481 ggcgatggcc gaggaagccc cggtcgtgga gctggtcagc aacctgatgg cgcaggcggt
    24541 cgagcagcgc gcctcggaca tccacctgga gccggaggaa cgcaccttca gcgtgcgctt
    24601 ccgcatcgac ggcgtgctcc acaaccgcct gtcgctgccg cgggaccgct tcgatgccgt
    24661 cgcctcccgg ctcaagctga tctcgggcat ggacatcgcc gagcgccggc tgccgcagga
    24721 cggccgcctg tccacccgcg tcggtggcca ggaaatggac atccgcgtct cggcattgcc
    24781 gggcgtgcac ggcgaatcga tcgtgatgcg cctgctgccg aaggagcgca aggagctggg
    24841 gctggagcgg ctcggcttcg aggccgacca cctcgcgatg atgaaggcct ggaccgccga
    24901 ggccaacggc atcgtgctgg tgaccggccc gaccggttcg ggcaagtcga ccacgctcta
    24961 cgcgtcgctg gccgcggcca acgacggcct caagaagatc atcacggtcg aggacccggt
    25021 cgagttccag ttgccggcga tcacccagat ccaggcgcat ccggacatcg gcctgacctt
    25081 cgccagtgcg ctgcgttcca tcctgcgcca ggacccggac gtgatcatga tcggcgaaat
    25141 ccgcgacctg gaaaccgcgc agatcgcagt gcaggcggcg ctcaccggcc acctggtgct
    25201 gagcaccctg catacgaacg atgcgatcag cgcgttcacc cgcctgatcg acatgggcgt
    25261 ggaaccgttc ctggtcgcca ccccggtgcg cgcggtgcag gcgcaacgac tggtgcgccg
    25321 cttgtgcccg cattgcgcgc ggccgcatgc ggttcccgcc gcgatcgagg ccgaagtgcg
    25381 cccgttcgcc gagcgcgtgc tggcgggcat ggcgccgcgc tggatggagg cggtgggttg
    25441 ctcgcagtgc atgaacaccg gctaccgcgg ccgcctgggc atctatgaaa tggtgccggt
    25501 gaccgaggcg atgcagcacc tgatcgtcag cggcgcatcg gtgaacgaga tgaagaagct
    25561 ggcccgcgac gaaggctgcc ggttcctgcg cgaggatggc ctgctgaagg cgtggcaggg
    25621 ccagaccacg gtcgaggaag tgcttcgcgt gaccggcagc tgagccccgc atgccccgtt
    25681 accactacca cgcactgaac gccgagggca tcagcctgga aggcatgctg cgcgccgaca
    25741 acgagcgcga cgtcgcccgc cagctggagc ggcgtggcct gtcggtggtc gaggtgcgtt
    25801 ccggcgaggc cgccggtcgc gaacgccgcg gtcgcctgcg ccatggggac gtgatcctgg
    25861 ccctgcagga actggccacc atgctgacct ccggggtgag catcgcggac gcggtcggca
    25921 gccaggcgct gggtgcgcac catccccgca tcgtggcggc attcaccacc atgtcgcgcg
    25981 acctgcagcg aggcctcgcg ttttcggcca cgctcgccaa gtgtggcctg ccgttgccgg
    26041 agtacgtggt gcaactggcg cgtgccggcg agctcaccgg cgaactcggc agggcgttgc
    26101 gcgatgcctc cacccagttg gagtacgagc agcagctgcg caacgagatg cgcaatgcgc
    26161 tgatctatcc cgcggtactg gtgctggccg gcgtggcggc agtggcgatc atgttcatct
    26221 tcgtggtgcc gaagttcgcc tcgctgctgc agcaagccga ggacctgccg ttgctggcgt
    26281 gggtggtgct agcgttcggc acctggacgc gcgaacactt cctgttgttg ctggtcctgc
    26341 tggcggcggc gggcggcggc gcggcgtggg ccctgcgcaa gccggagttg cgcgcccgcg
    26401 ggatggatgc gatgtcgcgc tggccgatcg tcgggccatg gctggtcgag gccgacaccg
    26461 cgcgctgggc caaggtcctc ggtgcgctgc tgggcaacaa ggtgccgctg atgcgcgggc
    26521 tggaactggc gcagtccggc ctgcaactgc cgcaacggcg cgcgcggatg ggcgaagtca
    26581 cccgcgcggt gcgcggcggt gcgtcgctgg cggatgcgct ggaagaccac gacgcgctga
    26641 ccgcgaccgg ttacaacctg atccgggtcg gcgagcgctc cggcaagctc gcatccatgc
    26701 tggattcatt ggcccggctg tatgaagagg ccggccgcaa ccgcatgaag cgcgtactga
    26761 tcctgctgga gccgatcgcg atcctggtga tcggcagcgt gatcggcacc atcatcctcg
    26821 gcgtgatcct ggccatcacc agcgccaacg acctcgcggt ctgaacgtcg ccgtactcaa
    26881 attcaggggc acatccatgc aacgtcgcat tcccgccgtt ccgcgccaag ccggcttcac
    26941 cttgctcgag atgatcgtgg tgctggtcat cattggcctg atcatgggcc tggtcggccc
    27001 gcggctgttc ggtcaggccg acagggccaa ggtgcagacg gcggtcaccc aggccaagat
    27061 gctgcgtggc gccttgcaga ccatgcggct cgacatcagc cgctggccca ccgatcagga
    27121 aggcctggcc ctgctgacca ccaagccggc gaatgccgat gccgcgcgcg gctgggccgg
    27181 cccttacctg gacgaagcgg tgccgcagga tccctggggc cagccgtacc agtactcgca
    27241 gcagcccagc ggcgagagcc ccatcaccct gtattcgttc ggcgccgatg gccgggccgg
    27301 cggcgaaggc caggatgcgg atgtcggcta tccgccgagc cagcccgcca cgccctgagc
    27361 aacccctgcg gatgcgccag ggcggcttca ccctgctgga gatgatcgtg gtgctcgcga
    27421 tcctcggcct ggccgcggcc ctggtcggcc cgtcgatgct gcgtggcatc gacagctggc
    27481 gccggcaggc ggcgatggac ggcctgctcg accagttgcg cgcactcccc ggcaacgccc
    27541 gcgcgagcgg caagccgatc acgctcagca cggccacgct tgcgtccacg acgccgcccc
    27601 tgcggatcga tggcgactgg accctcagcg tgccggagcc gtggtcggtc gccgccaacg
    27661 gcgtctgcca gggtggcgac gtggtggtgg cgaatgcctt cggcgaacgc accgtgcgcg
    27721 tcgctgcccc gttctgcgac ccgaccgtgc aaccgtgatg cgcctgcgtc gcgcgcctgc
    27781 ccggcagcat gccagtggct tcaccctgct ggaggcgatc gtcgcgctgg tggtcttcag
    27841 catgggcgcg ttcgcgctgt acggctggct gtcgaccaac ctgatcacgt tggagcgcat
    27901 ccgcgatcgc cagcaggtgg aggccgcgat gcattccgca ctcgacatcg tgcgccgcac
    27961 gaacccgatg gaaacgccga ccggacagcg tgcggtcgga gacctgatgg tcagctggac
    28021 cagcgtgccg atggaagcct tgcggccgaa caccgatcaa tcgggctacc ccggcttcta
    28081 cacggtagga ctgtacgacg ccaccatccg cgtcctccgc gatggccgcg agctgcatgt
    28141 gttccatgtg cgccagatcg gctggaaaca ggtccgcaac ggggcggagc catgaatgcg
    28201 tgcccggcac gcggtgcggc gggcaggtcg cccggtttca ccttgatgga aatgctggtc
    28261 accctggtgc tgatctcgtt cgcgaccatg ctgatgttcc agatgctcgg cagctaccgc
    28321 atcgccagcg aacgcgtgcg cgggcaggca ggccacatcg accgccaggc gctgttccag
    28381 tcctggttcc gcgacagcgt gcacgggctg tatgcggcgc ccaacctgca tttcatcggc
    28441 gacgccgcgc acttctccgc caccacgctg aacccgttgt atgcgccgga aggcagtccg
    28501 atcgcgatcg cctggtccct ccgcgagaac gcgcaaggtg ccgccgagat cgtctacagc
    28561 gaagacggcc gcgagcgctg gcagctgccg ctgcaaggcg gcggcgaagt cgagttcacc
    28621 tatttcgacg ccaattggcg cgcgagcgat gcctggccgc cgaaactcgg caaggtcaac
    28681 ccggaggcgt tgcccgcagt ggtcggcctg gtccgccgct acaaggacgg cgaccgtccc
    28741 ttattggtgg ccgtgctcgg cccgctcgag ccacctcgcc gcctgtatgg cgaggaggaa
    28801 ttgcaatgag gccggtgccg cgcgcgcgcg ggtttgtgct ggtgctggtg ctggcgatgc
    28861 tggtgatcct cggcttgctg gccggcacca tcgccgcggt gaccgggcgc cttgccgaac
    28921 aggcgcagca acgcgcgcag gcgatgcagg atgcggtgga gatcgccagc acgcgggcga
    28981 ccgtgctgta cctgctctcc acgcagcgca tgaccctggg cggcctgacc gtggacaggc
    29041 tggtgtcgtt cggcgaggac ggcgtgcgcc cgatgcaatc gctcgaggac ctggattcga
    29101 cgatgccggt cggcagcgaa ctccacctcg acggcagcgc gtaccagggc gtcggcggcg
    29161 tccgcttcgc gctgcaggac gactatgggc tcttcggcat caactggtat ccgcactggc
    29221 ggctggagaa actgctggcc cagggtgggc atccgcggcc ggtgccgacc gaggcgctga
    29281 tcaaccgcct gttcgactac caggaccgcg acgacctgta ccgcctcaac agcatggagg
    29341 cggacgggta ccgcaaggcc gggatgacgc cgcccaccaa cctgccgctg gccacgccga
    29401 tggaggtgat gcgggtcgtg ggttgggccc aggcgctgtc gttcctcagc ccggcggaga
    29461 tcagcgagac catcaccgtc gaagccgtgg gcggcatcaa tgtcaacacc gccccggcgc
    29521 gcgtgctgcg ggtgatcgac ggcgtcgacg aggaaaaagc cgcgcgggcg atcgccttcc
    29581 gcaaagtgca accgttcatg accgatgacg cctttttcac cttcctcggg ctgccgaagt
    29641 ccttggattc gccggtcgcc gtgtatcctg ccacgtcggg cactttgaag ctgtggcctt
    29701 cgcatggggg gcaggtggga ctggtgcact ggacgatgac cccgttcgag gacaagggtc
    29761 gcccctggcg cgaggactat gagctcatcc aatcccaagc cccgtcgcaa gacgacgttg
    29821 ctctcccggt tcgctcgcgg cttttcggcc agcaggtggc tgcgcggaag tgacggcctc
    29881 cgcccgttgg cggaaggcgg caacgggcag gaatggatcc tggcgcgtgg ctattgcgcc
    29941 tacacggtgc tggatggcgc ggcggtgccg ccgcgcaagc gccgcggttt cgtcgacatg
    30001 gcggtctcgc gctggtcgcc gttcgccgac gcgcaatcgc acgtggagtg ggtcggcgac
    30061 cgcgcgatgg tctgggcgtg gtcgaagtcg cgggtgctgg ccggtccgga agacaccctg
    30121 ctgccatcgc cgcggcgcag ctggcccgaa tcgctgttcc gcgggcaacc gcgcgccgag
    30181 ggcgaggaac tggtcgcgct ggacgaaggc ctggaggcgc gggcttggcg cgacggggtg
    30241 atgaccgcct cgcgctggtg gcccgaggcg ccggacctgc ccgaatggaa cgagttccgg
    30301 cgcggtgccg gcttggcgcc agcctcggcg atgccggtgc cggcgtcgta tcccctggtc
    30361 gatcatccat gggcggcacc gaaggcgatc gccctgggcg aggcctttgg gcattaccgc
    30421 gattacctgg cgatggcggt ggtcgggatc ggtgccgcgg tcatgtccgc cctgctggtc
    30481 ggcgtgctcg cgctcaaggt ctccaactgg cagctggacc gggacatcgc cgagcgcgaa
    30541 caggcgctgg aaaagatcat cgatgcgcgc gaccgcgcgc agcagggccg tgcggcgatc
    30601 gatgcgcggc tctccctgcg gccgccggcg gggcaggtcg aactgctctc gctggtgtcg
    30661 gggctgatgc gcgggaactg gcaactggtc gaatggaaga tgccagacgc acagacgctg
    30721 gaagtgaccg cgaagatgct caatgccgat cccggcgcga tcgtgtctgc ctgggaggca
    30781 tcgaagcgct tcaccgaagt gaccgcggag atcggcaagc aacccgaaac ggtggtggtg
    30841 aaggcccgca tcctgcgcgc cgcaccggcc ggggggagtc gcaagtgagc gcggccctgc
    30901 cggcgggtac ccgcaagatc cgcgaggaat ggcgcagcaa cccgcgcctg cgctggggcg
    30961 cggcgatcgc cgcggcgatc gggttcctgt acctgtgcct ggtactggcc gactggcgcc
    31021 gcgagctcca tgaggaatac cagcaacgca cgctgcagct ctacaagatg accgagctcg
    31081 ccggccagga gcagtggctg gtgcgtgccg aaggcgcgca ggcggtcgag aaggcgctgc
    31141 aggcggaaat cccgaacgcg gccacgatcg gactggcgca ggcggaagtg cagaccatgg
    31201 cccgccagct gatcaacgcc ttcggtcgtc ggttgagcag cgatgcacgc ccgccggcgg
    31261 aggtcaccgg ccagccaggg ctgtggcgcg tcccggtgac gctgcgtggc ctggtcagcc
    31321 agccccagat gctggagatc ctgcgccggg tggaggattc ggaccggctg atggtcgtcg
    31381 acgaattctc cttcaccttc gtcaagggcg tgcccaacat gtcgatgacg ctggtcgcgt
    31441 attaccgcgt cggtgcccct gcccagggag cgcgcaatgg cagtcgctga cctgctccgc
    31501 cgccatcgct tcctggccgt ggcgatcgcc ggtggcgttg tcggcagcgt gatcggactg
    31561 ttcctgccga tccgtgcggc ggatccgccg aaggccgagg aagcggcgtg gtcattgccc
    31621 aatgcgcagg cgctcaagcg cttccgcgac gaccagttcc aatccgtgcg cagggcgcgc
    31681 ttctggggcg aactggccat gcccggccgg cgcaatgccg agcagccgtc gtcgtggagc
    31741 ctgcatgcga tcgtgacccg cccggtcgtg caggtcggga ttggcgccaa cggcaagccg
    31801 gggtcttcat gggtgcggct tggcggtgcg ttgccggatg gcgcgaccct ggtggcggtg
    31861 gatcgcgacc gcatctggtt cgagaaggac ggttgtcgcc ggatgcgggc gttgtacccg
    31921 gacaagacca aatccgacgc ggatgcctgc atcggcgcgg cggacgatgg cacggcacct
    31981 gcctcgaccc cgccgccgag accgtcggca tcgcccgccg cggcgcccgt cgccggcaag
    32041 cccctctgaa ctccttttgc ggtgaatccc ccggcatgaa caatcttcgt cccttcctgg
    32101 tcgccaccgg catcgccctg ctcgcggggg cctgcgcaac gccccgcctt gcggttccgg
    32161 aacccttgcc ctcacccggg tcgtccagcc aggccgaaac cggcttcgac cagctcaatg
    32221 ggaccacgaa gcaggccccg ctgggcctgc agcgcagcgg tacgccgcag attccgggcg
    32281 agggaggcag ccttgtcgcg ggcgcgcagg gcgaaccgat gccgccgctc aagggtgggg
    32341 ccgtcaatgt caacatcgaa ggcatgccgg tgccggcctt catcaacgaa ttcttcggct
    32401 cgatcctggg gaccggcttc cagatggatc cgcaggtctc gcgcatgaac gacctggtga
    32461 ccctgcgaac ctcgagcccg caatcgccgc agaatttct
//
LOCUS       DAOOCX010000012        31449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1098_length_31449_cov_8.793846, whole genome shotgun sequence.
ACCESSION   DAOOCX010000012 DAOOCX010000000
VERSION     DAOOCX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 31449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31449
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1098_length_31449_cov_8.793846"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..618
                     /locus_tag="PK399_02130"
     CDS             172..618
                     /locus_tag="PK399_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HPW11958.1"
                     /translation="MRNAFIALSTLWLLAAAAAHGQQPHSSAVGRWKTIDDATGKAKS
                     VVEVYVAKDGRLAGKVVEILDLKDGPNPACTECKGTQNGKPIKGMVILWGLHADGTGR
                     WAGGRVLDPENGKDYKAKLELLDGGRKLGMSGCIAFICRQQAWVRE"
     gene            709..2790
                     /gene="metG"
                     /locus_tag="PK399_02135"
     CDS             709..2790
                     /gene="metG"
                     /locus_tag="PK399_02135"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPW11959.1"
                     /translation="MPRSALVTNALPYANGPLHLGHLVGYIQADIWVRARRMRGDTVH
                     FVCADDTHGTPIMLAAEKAGVTPEAFIAGIQASHERDFAAFGVAFDHYDSTNSPANRE
                     LTESFYARLEAAGHIGRRSVAQFYDPAKGMFLPDRYIKGICPNCGTADQYGDNCESCG
                     ATYAPTDLKEPKSVLSGATPEMRDSEHFFFEVGRFEPFLRDWLAGDVALPGVKAKLME
                     WLDAEGGLRAWDISRDAPYFGFEIPGQPGKYFYVWLDAPIGYLSSFKNLCAKNGTDFD
                     AFLAPGSDTELHHFIGKDIVNFHGLFWPAVLHGSGHRAPTRLHVNGYLTVDGAKMSKS
                     RGTFIMARTYLDVGLEPEALRYYYAAKSSGGVDDLDLNLADFTARVNADLVGKFVNLA
                     SRCAGFIEKRFDGRLADALPDAAQYARFVATLNGVREAYERNDPASAIRQLMALADEA
                     NKYIDEHKPWVLAKQDGADAELQAVCTQGLNLFRVLASGLAPVLPRTSAQAAAFLNAP
                     VRAWSDLDAPLVAHAINPYAPLFTRIDPKHIDAMTDASKDTLAAAPVAPAKPAEAKKA
                     EVASGAHGSTGSPRADAGIIGMDVFAKLDLRIGKVLECGFVEGSDKLLRFLLDAGDLG
                     QRQIFSGIRGSYGEPDALVGRNVVFIANLAPRKMRFGLSEGMILSAGFDGGALALLDA
                     DAAAMPGMPVR"
     gene            2791..3381
                     /locus_tag="PK399_02140"
     CDS             2791..3381
                     /locus_tag="PK399_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HPW11960.1"
                     /translation="MDLALVDFDHTVTTCDTYSRFLRRVATPTQQARAKWSIGPWLAG
                     YKLRVLSAEALRIRATRVVFSGRDAAEIDDAGERHARDLLPGVLRPAMMERIGWHQAR
                     GDTVVIVSASLDAYMRPWCAAHGLALICNHLEAVDGRLTGRYLGGDRGRHKAGDILAR
                     YDLASFDRVHAYGDSREDRAMLALAQERWWRGDRVA"
     gene            3399..3857
                     /gene="rnfB"
                     /locus_tag="PK399_02145"
     CDS             3399..3857
                     /gene="rnfB"
                     /locus_tag="PK399_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf electron transport complex subunit RnfB"
                     /protein_id="HPW11961.1"
                     /translation="MQDSSPPLVERLDRLLPQTQCGQCGYAGCRPYADAMARGEAGID
                     HCPPGGDAGARALARVLGVPAKPYDRSRGEQLPPQVAVVVEADCIGCTKCIQACPVDA
                     IVGASKAMHVVIDPLCTGCALCVPACPVDCIVMELLPQPAVARSQEMGSE"
     gene            complement(3946..4908)
                     /locus_tag="PK399_02150"
     CDS             complement(3946..4908)
                     /locus_tag="PK399_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPW11962.1"
                     /translation="MTPRLLTLALAAAFALPAHADQANKSAPSPIANAGAATVVARPL
                     LWKVSDADNDVYLLGSFHLLKSDDYPMPVEIDRAFDDAESLLFEVDPREMTSPETAAI
                     MQKYMAYEDGQSLSKVLPKATLERLHTLVSASGGSVQALEQSEPWLVNLGLVLGVSQA
                     MGLKADLGLDRHLMERAAKAGKPAGGLETIDAQMQAMDSVPYPEQAQGLDEFLVDPKK
                     AVQQLQDMHAWWRAGDVERLDTGMRAEMAAKSPQSYKLLDVDRNEAWLPQIEKRLGGS
                     TSDDTLVVVGALHLLGSDGLVEKLKAKGYKVERVCDGCEAAATK"
     gene            complement(4905..5870)
                     /locus_tag="PK399_02155"
     CDS             complement(4905..5870)
                     /locus_tag="PK399_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HPW11963.1"
                     /translation="MKSAGIALACVLAMAGCERAPPPQATPDEAAQLQAALKQYAHDQ
                     FMWRGRRVIDLSRADGWTSLVGMHWLDPGAHRVGRDVDNGIRLAMGPEHLGVFTVRDG
                     KASFVADVPVTVDGKPARGGVLRSDKDPAGPSVIAFDEGKGLVTVIERGGRLALRVKH
                     ADAPSRVHFAGLEYWPGGRDWQVPARFIAHPAGKTLPIANIIGTIDHIPNPGALEFQR
                     DGKTYRLEALDEGEDTLFLVFADRTSGHGSYGAGRFIDAPKPDAQGRVLIDFNQAYNP
                     PCAFTPFATCPLPPPENRLDLAVSAGEKAYAKPDDTSIPPPKDPA"
     gene            complement(5867..7675)
                     /gene="mutL"
                     /locus_tag="PK399_02160"
     CDS             complement(5867..7675)
                     /gene="mutL"
                     /locus_tag="PK399_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPW11964.1"
                     /translation="MTIRQLPDTLINQIAAGEVVERPASVVKELVENALDAGARRIDI
                     DLEDGGIRLIRIRDDGGGIDPAELPLAVQRHATSKIASIDDLEAVATLGFRGEALPSV
                     ASVSRFRLTSRRNGNEHGALLQVEGGKVGEVVPHAHPPGTTVEVRDLFYNVPARRKFL
                     RAERTELSHIEDWLRTLALARPDVELRVAHNGKASRRWKGEAQLQEGSMLSDVRLHEA
                     LGEDFTRNALRVDHAGAGMRLHGWIAQPAYNRASADQQYLYVNGRSVRDRNVSHAIRQ
                     AYADVLFHGRHPAYVLFLELDPRGVDVNVHPAKHEVRFRESRLVHDFVYRTLHAALAE
                     TRAGGAATAMQPMQAPHAWSMPAPQQSSIGLRVADAPSAYAALYAPRDGGSFADAAES
                     PATVAAMPMPDDAPGGMPPLGFAVAQLHGIYILAENADGLVVVDMHAAHERIGYEKLK
                     AAHDGAGLRTQPLLVPSSLAVSEREADVAEREAGTLARLGFEVQRSGPQSLLLRSVPA
                     LLAHGDVEALLRDVLTDLREHGTTRRVAETRDELLSTMACHGAVRANRRLTLHEMNAL
                     LREMEATERSGQCNHGRPTWTRFGLAEMDRWFLRGR"
     gene            complement(7831..9222)
                     /locus_tag="PK399_02165"
     CDS             complement(7831..9222)
                     /locus_tag="PK399_02165"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPW11965.1"
                     /translation="MLLAALCWNLANASEIKQLRVDAGATGTRVELQLDRSGDYKVIE
                     LRNPDRLVVDFPDSRLARNLALPAGAGAVKAVRTGQPQPGTIRVVFDLASQVKAMPPR
                     IETTAAGPLLVLEWPGDGVASAVVVPAAPVAAARPDPIAEIAKSSAAVETDPGKLVGK
                     AATVSELPGTVATGVPTRIATGKPEPIASETATVAAPVTRAAMQAGMRPLVISIDAGH
                     GGQDPGAIGPNGNREKNVTLAIARELARQINATPGMKAHLTRDTDVFIPLNRRAVLAR
                     GARADIFVSIHADAAENRDATGSSVYVMSTRGASSQRARWLADKENAADLIGGVKLAA
                     TGDTLKSVLLDLTQSGQMKASSDAAAHVLGSLGSVGKTRRVEHANFAVLRTSDVPAML
                     VETGFISNHGEEALLSSPDYQRKLAAAVLAGIDQYFTRQPPPGTLYAARAQATQVSQA
                     STSGAGASAGGSP"
     gene            complement(9374..9829)
                     /gene="tsaE"
                     /locus_tag="PK399_02170"
     CDS             complement(9374..9829)
                     /gene="tsaE"
                     /locus_tag="PK399_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPW11966.1"
                     /translation="MHDVFLENADATDALGARLASALPPHAVAYLHGDLGAGKSTLAR
                     ALLRALGVTGTIRSPTYTLVEHYPLPNGGVALHLDLYRIGDPGELEFLGLDPAETRLW
                     LVEWPERGQGGLPAADLDVVLAVEGTGRRCHLQPRTPGGEAWLQALGSA"
     gene            complement(9834..11321)
                     /locus_tag="PK399_02175"
     CDS             complement(9834..11321)
                     /locus_tag="PK399_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPW11967.1"
                     /translation="MHSPFPLYAPDQLRELEARGIAACGGDGFVLMARAGLAAWQQLL
                     KHWPHARRLVVACGPGNNGGDGYVLARHALASGLDARVLRLDAHAPRTPLAMQAHDAF
                     VGAGGQVECFDGDLGGADVLVDAVFGIGLSRAPDAHANALIEAINRHPAPVFSLDVPS
                     GIDARSGDAPGVAVIAARTLQFIARHRGLRTGIALDHAGDLALAALDLPETVFEGIEP
                     AALALRADALPGFFSLRPRDSHKGTNGHVLCIGGDHGSGGAILLAAEAALRSGAGLLS
                     VATRASHVPALLARRPEAMAHAVDDVAQLAPLLARAGVIALGPGLGQGEWGRQLLEAV
                     LASEAPRVVDADALNLFASSGHRLRAQDVATPHPGEAARLLGIPTSEVQRDRFAAARA
                     LQERLGCVVVLKGAGSIIASANAPSTVICAGNPGMAVGGMGDVLTGVIAALLAQGMTS
                     ADAASAGALLHAAAGDAAAREDGERGLLPSDLMPWLRRLANPMRR"
     gene            11357..12427
                     /gene="queG"
                     /locus_tag="PK399_02180"
     CDS             11357..12427
                     /gene="queG"
                     /locus_tag="PK399_02180"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPW11968.1"
                     /translation="MSSPLPDYIALADRIRALALAAGFQRVGISGVELQEDETFLQSW
                     LAEGLHGSMAWMASHGDKRSRPAELIPGTLRVVSVGMDYGKDTDAAWDNLRDGERAYV
                     ARYALGRDYHKLMRNRLQRLADDVAKEIGPFGHRVFVDSAPVLERALARNAGLGWIGK
                     HTCLIDRDGGSWFFLGELFVDLPLPIDPPASAHCGSCTRCIEVCPTQAIVAPYRLDAR
                     RCISYLTIEHEGSIDEALRPLIGNRIFGCDDCQLVCPWNKFAMRSDEPDFRVRNDLDR
                     ATLVDLFAWEEDEFLRRTEGSAIRRSGHERWLRNIAVALGNAPKTPLVMAALESRRKH
                     ASPVVREHVEWALRQHTANEAP"
     gene            complement(12553..14049)
                     /gene="pgi"
                     /locus_tag="PK399_02185"
     CDS             complement(12553..14049)
                     /gene="pgi"
                     /locus_tag="PK399_02185"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPW11969.1"
                     /translation="MLAGLDAEARRLASTTITDLIREDPARASDFALRCGGLYANFAR
                     QRYGRAALDALFAIGERAELMVAMRRLLDGALVNPTEGRAALHSALRGDNSTSQVAVE
                     ARAQAVAAQARMRVLIEQLEASDVTDIVSVGIGGSDLGPRLMVDALALHDARFKVHFI
                     SNVDGNAAQRVLAGLDPKRSAAIVISKTFGTQETLLNGGIVRAWLGNDERLYAVSTNA
                     ERVAAFGVPPERTLPMWDWVGGRYSLWSAVGFPIALALGMDRFLELLDGAAAMDAHAA
                     TAAPRGNIAVWHALTAVWNRNALHAATQAVLPYDDRLKLLPAYLQQLVMESLGKSVRI
                     AGGAVDVDTVPVWWGGAGTDTQHSFFQALHQGTQIVPADFIGVLRADHPHAQNHAALL
                     ANLLAQTEALANGQDSDDPHRRYPGNRPSTLLLLDAVTPHAVGMLVALYEHSVYLQSV
                     LWGINAFDQFGVELGKQVASRLLPALQGDAEADDAVTRALLREFSQRG"
     gene            complement(14052..14432)
                     /locus_tag="PK399_02190"
     CDS             complement(14052..14432)
                     /locus_tag="PK399_02190"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPW11970.1"
                     /translation="MQLTLLKAKIHRASVTHAELHYEGSCAIDGRLLDISGIREYERI
                     EIYNVNNGERFATYAIRGEEGSGVISVNGAAAHKAQPGDLVIICAYGQCDEAEAAKFK
                     PTLVYVDRDNALTHTNRSMPKQAA"
     gene            complement(14539..15393)
                     /gene="panC"
                     /locus_tag="PK399_02195"
     CDS             complement(14539..15393)
                     /gene="panC"
                     /locus_tag="PK399_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPW11971.1"
                     /translation="MIETITALESLRSRIRGWKRDGLRVGFVPTMGNLHAGHYSLVAL
                     ARQHADKVVSSVFVNPTQFGPNEDFTRYPRTPDADTSGLQSAGCDVLWLPDVAAMYPF
                     GVDLAASVHVPGVSAVLEGAHRPGHFDGVCTVVSRLFNQVQPDVAVFGKKDYQQLAVI
                     RQMVADLHVPIDIVGGDIVREANGLAMSSRNQYLSPQEREVSAIIHHTLHAMRDGLLV
                     GLPLAQVEADADEALRDAGFVPDYAVVRRPDLTEPMDGEAGDLVALIAARLGRTRLID
                     NLEFHLAG"
     gene            complement(15416..16225)
                     /gene="panB"
                     /locus_tag="PK399_02200"
     CDS             complement(15416..16225)
                     /gene="panB"
                     /locus_tag="PK399_02200"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPW11972.1"
                     /translation="MSEPQKRWTVPMLGEARAAGRKLVMLTSYDASFARVLDDNGIDL
                     ILVGDSLGMVVQGFDSTLPVRVDDIVYHTAAVARGATRALKIADFPFGSDGSPQEAHA
                     AAVRFIQAGASMVKLEGAGHKLETIRYLVEREIPVCAHLGLTPQSVHRFGGFKVQGRE
                     DAAAAKLREDALAVADAGAALLVLEGVPAALAAQITAASPIPTIGIGAGAGCDGQVLV
                     LHDLLGLDTGHRKPKFVKDFLAEGGSVAAAIRAYADAVRDGRFPAAEHAYA"
     gene            complement(16249..16734)
                     /gene="folK"
                     /locus_tag="PK399_02205"
     CDS             complement(16249..16734)
                     /gene="folK"
                     /locus_tag="PK399_02205"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPW11973.1"
                     /translation="MTLAAVGLGANLGNAARTLRDAIDALDNVAGTRLLRASRLYRTP
                     AWGVTDQPDFVNAVAVVDTTLSARDLLDVLLAIERDAGRVRIEGERWGPRILDLDLLL
                     HGDAIIDEPGLRLPHPHLHERAFALLPLLEAWPDAGIPGIGPARECAGVMAADGIEPL
                     P"
     gene            complement(16731..18131)
                     /gene="pcnB"
                     /locus_tag="PK399_02210"
     CDS             complement(16731..18131)
                     /gene="pcnB"
                     /locus_tag="PK399_02210"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HPW11974.1"
                     /translation="MTEIESQPLYPVPSLRVIARDEHGLSRKQMSQNALRVLYRLREA
                     GFGGYLVGGAVRDLLVGGAPKDYDVATDATPEQVKSLFRNCRLIGRRFRLAHVVYGRE
                     IIEVATFRANIDDGSGDREQHEGGRLLRDNVYGTIEDDAIRRDFTANALYYAIEDFSV
                     RDYVGGYDDVQARLLRLIGDPEARYREDPVRMLRAARLAAKLDFSIEAATAEPIARLA
                     PLLAEAAPARLFEETLKMFLSGHAVASFEQLEAHGLLPALLPETAQALASNKSGALRR
                     MLVQGLRNTDARVAADESVSPAFLYAVLLWPAYCRALALLQAQGVHAADAQRRAADRV
                     TLHQVGRTALPRRFSLPMQEIWLLQPRFSQRVRKRVFRLLAHPRFRAAFDFLELRLAA
                     SDSHAEDVAYWREAQLHPSATVAAGEQGALDAGADGFDDAAADAGGEGDAPRKRRRRR
                     RRGGADATGAASQEPA"
     gene            18271..18346
                     /locus_tag="PK399_02215"
     tRNA            18271..18346
                     /locus_tag="PK399_02215"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18305..18307,aa:Glu,seq:ctc)
     gene            18648..18971
                     /locus_tag="PK399_02220"
     CDS             18648..18971
                     /locus_tag="PK399_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HPW11975.1"
                     /translation="MPFVVTENCIKCKHTDCVEVCPVDCFHEGPNFLVIDPDECIDCT
                     LCEPECPVNAIYPEEDVPAGQEVFVALNAELAKEWPVLATRKDAPADAAEWDGKPGKL
                     ALLER"
     gene            complement(19038..19556)
                     /locus_tag="PK399_02225"
     CDS             complement(19038..19556)
                     /locus_tag="PK399_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11976.1"
                     /translation="MRRSARVSPIAAPLAAALLVAGLSGCHWFGKKNELYTQSAETRP
                     LEVPPDLDRPSADKAMSLPAAGGSVSASGMNNAGGSSAAPIGFNASGDRDTLFAKVGE
                     VLAGTPGVTIANKAQILGTYDVDYMDAKFLVRVTKAGEGVYISAVDPRGLPPIGEAPV
                     KLIGALKAAIAP"
     gene            complement(19589..20470)
                     /gene="dapA"
                     /locus_tag="PK399_02230"
     CDS             complement(19589..20470)
                     /gene="dapA"
                     /locus_tag="PK399_02230"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPW11977.1"
                     /translation="MRLSGSITALATPFTAAGDIDFPAWQRLLQAQRDAGTQGVVVAG
                     STGEAAALFDDEYDALLASAVEFLGGRMPVLAGTGHSNTAKTILANRRAAALGADAAL
                     VVTPPYVRPTQSGLLAHFRAVADEGGLPVVLYNVPGRTGCDLAPETTGELSLHPNIVG
                     IKEARADAARMEDLLPCRRDGFAVLSGDDPTAVRAMLAGADGVISVASNVVPGTFRRL
                     CQLARGGDVAGAEALDARLYALYHFLGVEPNPIPVKALLALNGIGHGLRLPLQPLSST
                     HAAEAQRLHADILQLED"
     gene            20579..21190
                     /locus_tag="PK399_02235"
     CDS             20579..21190
                     /locus_tag="PK399_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein R"
                     /protein_id="HPW11978.1"
                     /translation="MRRIPPAPPTPPASDAPLTDISARPAPNENHLLINAYTTHPQSP
                     LLAVTRRISDSGCNLVDTRLATVGRDVSVTALATGSWDAVAKLETMLAKLEREEGLKL
                     IWYRTGPKPVQSSLLPYVVEVIASDKPGILFQLADFFDQQGITIESLHCSRYRAMQTG
                     ADMFSAQLTIGVPSDMHIAALRDDFLEFCDHLNLDAIMDPMKY"
     gene            21209..21334
                     /locus_tag="PK399_02240"
     CDS             21209..21334
                     /locus_tag="PK399_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11979.1"
                     /translation="MEFVLFFLAVAMIAGQHGEISKLKKRIDALETSPPAAADTP"
     gene            21331..21810
                     /locus_tag="PK399_02245"
     CDS             21331..21810
                     /locus_tag="PK399_02245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPW11980.1"
                     /translation="MIAGAALHGAVSRLPLALSDGTSATLADYRGKWLVLYFYPRDST
                     PACTTEGLDFNALLPAFKRAGAVVLGVSKDSLKSHQNFCAKQGFKFDLVSDADEAVCN
                     AFGVIQPKKLYGREYIGIVRSTYLIDPEGVIRQAWQPVKVPGHAQAVLDALKAAKRQ"
     gene            21932..23329
                     /locus_tag="PK399_02250"
     CDS             21932..23329
                     /locus_tag="PK399_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010365742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPW11981.1"
                     /translation="MTRSKRIYVLDTNVLMHDPTALFKFEEHDVYLPMQVMEELDNGK
                     KGTSEASRNARQVSRFLNELIEAHGSSDVHNGIALVRPQALQLRGAESAGRLLFQTGD
                     FDAGKRFGTVIPDNNILGAILALKESDPGAPVVFVSKDINLRIKASIAGIVSEDYEND
                     RALDDFSLLYTGATALPEDFWSRHGKDLKSWTDKGRTYYEIARGEDEDWYPNQFVYLP
                     GDEQAEMKVAKVADDKVVLQIVDDFRHASRAVWGITARNREQNFALNALMDPEIDFVS
                     LLGTAGTGKTLLALAAGLAQTMDAQRYREIIMTRATVSVGEDIGFLPGTEEEKMTPWM
                     GALTDNLEVLTHNQDGGAWGRAATNDLLASRIKIRSMNFMRGRTFLSRYLILDEAQNL
                     TPKQMKTLITRAGPGTKIICLGNVEQIDTPYLTETTSGLTYAVDRFKAWAHSAHITLR
                     RGERSRLADYASEVL"
     gene            23432..23683
                     /locus_tag="PK399_02255"
     CDS             23432..23683
                     /locus_tag="PK399_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW11982.1"
                     /translation="MISSAQMRAARALLGIDQKQLATLAGVSVPTIQRMESSQGTVRG
                     VVGTLTKIIEALDAAGIELIGNDHPSQGRGRGVRLKEPQ"
     gene            23683..25377
                     /locus_tag="PK399_02260"
     CDS             23683..25377
                     /locus_tag="PK399_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPW11983.1"
                     /translation="MAGRMDAARRAWYGMFEPKLLTVLREGYGLQAFKADAIAGLTVA
                     IVALPLAMALAIASGTTPEKGLHTAIIAGFLISFFGGSRVQIGGPTAAFIPVVFVVLQ
                     KFGYGGLILCTLLAGLMLIGAGLLRLGTLMKYMPQPVITGFTAGIAVSIFSSQVKDVL
                     GLRMETVPAEFLARWTAYGKHIATTQPAAVGLTLLGLVVIAGLRRWKPTWPGFLIALL
                     ACTLASAAFALPAETIGTRFGTLPSALPTFAIPHIPFERTFELLPSAFTIAFLAGVES
                     LLSAVVADGMTGGRHRSNGELVAQGIANVGSALFGGLPATGALARTATNIRAGGRSPV
                     AGILHAGYLLLFMLLLAPLMRHVPLAALAAVLLVVAWNMSEQEAFRNTLSAPKGDRVV
                     LLLTFLLTVFIDLTIAIEVGIVVAAFVFMFRMADAVEVSSAIGQGDAGDQRSRLPAGV
                     EVYQISGPLFFGTANRLDNLLDQFLSTPKVFILRMRLVPVLDASGVHALQKLADRCQR
                     KGIALVISGLQEQPNRVLARMQTHAHPAHMHLAPDFDHALKLAERLARQPATAPAQ"
     gene            25382..26188
                     /gene="thiD"
                     /locus_tag="PK399_02265"
     CDS             25382..26188
                     /gene="thiD"
                     /locus_tag="PK399_02265"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HPW11984.1"
                     /translation="MQHDRPPSALSIAGSDSGGGAGIQADLKTFAAHGVHGVSAIAAL
                     TAQHTRGVTAVHVPDVGFLRAQLDACFEDFDVGAVKIGMLATAGVIDCVADALEQYRP
                     RFIVLDPVMVATSGAKLLADDALHALRTRLLPLASIVTPNIPEAGLLTGLAIADGNDA
                     GNAIAALRAMGARAVLLKGGHLHEGGTVIDRYDDGAVDAEFTHDRLALEGHGTGCTLA
                     SAIAANLCLGMPMPLAVEAATDYIERALRGGYRPGRGDVVVLDHFGAAAR"
     gene            26188..27015
                     /locus_tag="PK399_02270"
     CDS             26188..27015
                     /locus_tag="PK399_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW11985.1"
                     /translation="MRAVELPVETGDGHRCELIARIPEAPRGRVLWLAGMGMPARHYL
                     PFADALAARGMAVFLHEWRGIGSSNVRASRTDDWGYRELLADLAASQAVADAQVPGLR
                     RVLGGHSLGGQMSCCQLGLHPDSADALWLVASGAPYWRVFGAPKRWGLLSAYRFMRWL
                     AARNGFMPGRRIGFGGNEARSVIADWARTAISGHYAAPGVDVDLEAGMARFGGDVRAL
                     TFDQDWLAPPESTAFLTGKLANAQVTAGHFDAAMAGTVADHYAWIKSPDASADWLLR"
     gene            complement(27020..28228)
                     /gene="fabB"
                     /locus_tag="PK399_02275"
     CDS             complement(27020..28228)
                     /gene="fabB"
                     /locus_tag="PK399_02275"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HPW11986.1"
                     /translation="MRRVVVTGMGIASCLGNDLGTVSSALRESRPGIRFLPDHAERGL
                     RSQVGGAVELDLDAVVDRKLKRFMSDAAAYSYVSLRDAIADAGLDEAAVSDPRTGLIA
                     GSGGGSSRWQVETADLLRDRGVRKVGPYMVPRTMCSTVSANLATAFKIKGLSYSLSAA
                     CATSAHCIGAAADLIRHGAQDIMFAGGGEDLDWTMSVMFDAMGALSTSFNETPANASR
                     PYDANRDGFVIAGGGGMLVLEDYEHAKARGAHIHAELIGYGVTSDGADMVAPSGEGAV
                     RCMRMAMAGIDRPIDYLNTHGTSTPLGDITELNAVREVFGDAIPPLSSTKALSGHSLG
                     AASVHEAIYSLLMLRDGFIAGSANIETLDPRCEGFPIVRDTRDAKLDTVMSNSFGFGG
                     TNAALVFARV"
     gene            complement(28237..28752)
                     /gene="fabA"
                     /locus_tag="PK399_02280"
     CDS             complement(28237..28752)
                     /gene="fabA"
                     /locus_tag="PK399_02280"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HPW11987.1"
                     /translation="MTRLSSLDREQLLACARGELFGEGNARLPAPPMLMFDRITHISD
                     DGGAHGKGFIRAELDIHPDLWFFGCHFLGDPVMPGCLGVDAMWQLTGFFLPWLGETGR
                     GRALGAGEIKFTGQVLPDAKVVSYEIDIRRLLRGRLRLVVADGRTYVDGREIYTARDM
                     RVGLFQSTEAM"
     gene            28934..30403
                     /gene="zwf"
                     /locus_tag="PK399_02285"
     CDS             28934..30403
                     /gene="zwf"
                     /locus_tag="PK399_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HPW11988.1"
                     /translation="MTHAAPASLLTIFGATGDLARRMLLPSLYSLQAEQLLPANLRIL
                     GTARSELDRDGFANLVSASIAERIPAAERDDAALRGLLDRLDYAAASVDDDASMAALA
                     SKVEALRDGDVLYHMSTAPKFYGPACQSLAAHGAAGAGTRVMLEKPIGKDLASAIAIN
                     DSVAMAFDEERIYRVDHYLGKEGVQNLIALRFGNALFEPLWNTRHIEQVQITVGETVG
                     VEGRGDYYDDSGALRDMLQNHLLQLLCLVAMEPPAKFDPTAVRNEKIKVLRSLRPIGR
                     EQVAHESVAGQYTAGASAGVAVPGYADELGRSSRTETFVALRAHVDNWRWSGVPFYLR
                     TGKRLPSRSTEIYIQFRKVPYSIFGGRAAGDMRPNGLLIRLQPEERIELDLMAKTPGL
                     DRSGLRLSQVGLDLDFHEEFANARKRIAYERLYLDAIEGNGTLFVRRDETEAAWQWVD
                     AIAEGWRAAGITPKPYPAGTWGPGAAVSLTERHGHGWRE"
     gene            30408..31106
                     /locus_tag="PK399_02290"
     CDS             30408..31106
                     /locus_tag="PK399_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPW11989.1"
                     /translation="MPWIEHDHADVGALVAHVADVFVEASREAIHARGQAWLALAGGR
                     TPLPVYARLAGVGLGGVVSAIPTDERCVPHDHPACNLRALRDAFAADANIVANPLTRE
                     DGDVDASLAQARALLAVNPQAFDAVLLGMGADGHFASLFPGAANLAEGMAIDSGIDAI
                     ATLPDPLPPEAPFARISLTLPRLLHARAIHLVVTGADKRVVLGNAQHDAASKFPVAAL
                     LHAPGPHVHIHWSP"
     gene            31109..>31449
                     /locus_tag="PK399_02295"
     CDS             31109..>31449
                     /locus_tag="PK399_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015472018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HPW11990.1"
                     /translation="MPLHPAIERVTSRIADRSRRLRGDYLARIDAAREAGPARRELSC
                     GNLAHGFAASGVDKPMLREGAGGNIGIITSYNDMLSAHQPMEHYPTLIRMAARNAGGS
                     AQVAGGVPAMC"
BASE COUNT         5005 a        10949 c        10596 g         4899 t
ORIGIN      
        1 gatcgacggc ggcaagaagc tcgaagtgaa gggctgcgtg tcgatcatct gccgcaccgc
       61 gacctgggtg cgcgcgaact gagcgcatcg ccctggacaa cgaaacggcc cgcttgcggg
      121 ccgtttccat ttcagggcga cgacctgacc acctccaacg ggaatccacc catgcgcaac
      181 gcgttcatcg ccctctcgac cttgtggctg ttggcggcag cggccgccca cgggcagcaa
      241 ccgcactcgt ccgcggtggg ccgctggaag accatcgacg acgccaccgg caaggcgaaa
      301 tcggtggtcg aggtgtacgt ggcgaaggac gggcggctgg ccggcaaggt ggtcgagatc
      361 ctcgacctga aggacggccc gaatcccgcc tgcaccgagt gcaagggcac gcaaaacggc
      421 aagccgatca agggcatggt gatcctgtgg ggcctgcacg cggatggcac cggcaggtgg
      481 gcgggcggcc gcgtgctgga tcccgagaac ggcaaggact acaaggccaa gctcgagctg
      541 ctcgacggcg gccggaagtt gggcatgtcg ggttgcatcg ccttcatctg caggcaacag
      601 gcctgggtcc gcgaataagc cggccacgat gccgcgcgca agccccgccc ggtcggggct
      661 tttttgtgcg catgcgataa tcgcggcccc ttcgcatcgc ctgcctccat gcctcgttcc
      721 gcgctggtca ccaatgccct gccctacgcc aacggcccgc tgcacctggg ccacctggtc
      781 ggctacatcc aggccgatat ctgggtgcgg gcgcggcgca tgcgcggcga cacggtgcac
      841 ttcgtctgcg cggacgacac ccacggcacg ccgatcatgc tcgccgcgga aaaggccggg
      901 gtcacccccg aagccttcat cgccggcatc caggccagcc acgagcgcga cttcgccgcc
      961 ttcggcgtgg ccttcgacca ctacgattcg accaactcgc cggccaaccg cgaactgacc
     1021 gaatcgttct acgcgcggct ggaagccgcc ggccacatcg gccgccgcag cgtggcccag
     1081 ttctacgatc ctgccaaggg catgttcctg cccgaccgct acatcaaggg catctgcccg
     1141 aactgcggca ctgccgacca gtacggcgac aactgcgagt cctgcggcgc cacctacgcg
     1201 ccgaccgacc tcaaggaacc gaagtcggtg ctctccggcg caaccccgga aatgcgtgac
     1261 tccgagcact tcttcttcga ggtcggccgc ttcgagccgt tcctgcgcga ctggctggcc
     1321 ggcgatgtcg ccctgcccgg cgtgaaggcc aagctgatgg agtggctgga tgccgaaggc
     1381 ggcctgcgcg cctgggacat ctcccgcgac gcgccgtatt tcggcttcga gatccccggc
     1441 cagcccggca agtatttcta tgtctggctg gacgcgccga tcggctacct gagcagcttc
     1501 aagaacctgt gcgcgaagaa cggcaccgac ttcgatgcct tcctcgcgcc cggcagcgac
     1561 accgaactgc accacttcat cggcaaggac atcgtcaact tccacggcct gttctggccg
     1621 gcggtcttgc atggcagcgg ccatcgcgca cccacccgcc tgcacgtcaa cggctacctg
     1681 accgtggacg gcgcgaagat gtcgaaatcg cgcggcacct tcatcatggc gcgcacctac
     1741 ctggatgtcg ggcttgagcc ggaagcgctg cgctattact acgcggccaa gtcctccggc
     1801 ggcgtggacg acctcgacct gaacctggcg gacttcacgg cgcgcgtgaa cgccgacttg
     1861 gtcggcaagt tcgtcaacct ggccagtcgc tgcgccggct tcatcgagaa gcgtttcgat
     1921 ggccgcctcg ccgatgccct gcccgatgcc gcgcagtacg cgcgtttcgt ggcgacgctc
     1981 aatggcgtgc gcgaggccta cgagcgcaac gatccggcct cggcgatccg ccagctgatg
     2041 gcgctggccg acgaggccaa caagtacatc gacgagcaca agccgtgggt gctcgccaag
     2101 caggacggcg cggatgccga actgcaggcg gtctgcacgc agggcctgaa tctcttccgc
     2161 gtgcttgcgt ccgggctggc tcccgtgttg ccgcgcacca gcgcgcaggc cgcggcattc
     2221 ctcaacgcgc cggtgcgcgc gtggagcgat ctcgatgccc cgctggtggc gcatgccatc
     2281 aacccctatg ccccgctgtt cacccgcatc gaccccaagc acatcgacgc catgaccgac
     2341 gcctcgaaag acaccctcgc cgccgcgcct gtggctcccg cgaaaccggc cgaggcgaag
     2401 aaggcggagg ttgcctcggg cgctcatggt tcgacaggct caccacgagc ggatgccggc
     2461 atcatcggca tggacgtttt cgcgaagctc gacctgcgca tcggcaaggt gctggagtgc
     2521 ggtttcgtgg aaggctccga caagctcctg cgtttcctgc tggatgccgg cgaccttggc
     2581 cagcggcaga tcttctccgg catccgcggc agctacggcg agccggacgc ccttgtcggc
     2641 cgcaacgtgg tgttcatcgc caacctggcc ccgcgcaaga tgcgcttcgg cctgagcgag
     2701 gggatgatcc tgtcggccgg tttcgatggc ggcgcgctgg cgctgctgga tgccgatgct
     2761 gcggcgatgc cgggcatgcc ggtgcgctga atggacctgg cgctggtcga tttcgaccac
     2821 accgtcacca cctgcgacac ctactcgcgt ttcctgcgcc gcgtcgccac ccccacgcaa
     2881 caggcgcgtg cgaaatggag catcgggccg tggctggccg gttacaaatt gcgggtgctt
     2941 tctgcggagg ccctgcgcat ccgtgcgacc agggtcgtgt tcagcgggcg cgatgccgcg
     3001 gagatcgacg acgccggtga acgccatgcc cgcgacctgc tgccgggcgt cctgcggccg
     3061 gcgatgatgg aacgcatcgg ctggcaccaa gcgcgcggcg acaccgtggt gatcgtgtcc
     3121 gcctcgctgg atgcctacat gcggccatgg tgcgccgcac acgggctggc gctgatctgc
     3181 aaccacctgg aggcggtgga tggccgcctc accggccgct atctcggtgg cgatcgcggc
     3241 cggcacaagg ctggcgacat cctcgcccgc tacgacctcg ccagtttcga tcgggtgcat
     3301 gcctacggcg acagccgcga ggaccgggcc atgctggcac tggcgcagga gcgctggtgg
     3361 cgcggcgatc gcgtcgcctg agcgttacag tcccaccggt gcaggactca tcgccgccgc
     3421 tggtcgaacg cctcgaccgc ctgttgccgc agacccaatg cgggcagtgc ggctatgccg
     3481 gctgccggcc gtatgccgac gcgatggcgc gtggcgaagc cggcatcgac cattgcccgc
     3541 ccggcggcga tgccggcgcg cgggcgctgg cgagggtcct gggcgtgccg gcgaagccct
     3601 acgaccgcag tcgcggcgag caactgccgc cgcaggtggc ggtggtggtg gaagccgact
     3661 gcatcggttg caccaagtgc atccaggcgt gcccggtcga cgccatcgtc ggcgcatcga
     3721 aagccatgca cgtggtcatc gatccgctgt gcaccggctg cgcgctgtgc gtgccggcct
     3781 gcccggtgga ttgcatcgtg atggagctgc tgccgcaacc tgcggtggcg aggagccagg
     3841 aaatggggtc agagtgaatt ttgcgccgac catcgtcggc tcaaccaaga atcttggtcg
     3901 caaaacttca ctctgacccc atttccggat cgccgcgagc aatcgctatt tcgtggccgc
     3961 agcctcgcag ccatcgcaca cccgctcgac cttgtagccc ttcgccttca acttctcgac
     4021 caggccgtcg ctgccgagca agtgcaaggc gccgaccacc accagggtat cgtcggaggt
     4081 ggagccgccc agtcgtttct cgatctgcgg cagccacgct tcgttgcgat ccacgtccag
     4141 cagcttgtac gactgtggtg atttggcggc catctccgcg cgcatgccgg tgtcgaggcg
     4201 ctccacgtcg ccggcgcgcc accaggcatg catgtcctgc aattgctgga ccgccttctt
     4261 cggatcgacc aggaattcgt ccaggccctg cgcctgttcc gggtacggca ccgagtccat
     4321 tgcctgcatc tgcgcatcga tcgtttccag cccgccggcc ggcttgccgg ccttggccgc
     4381 gcgctccatc aggtggcgat ccaggcccag gtcggccttc aggcccatcg cctggctcac
     4441 gcccagcacc aggcccaggt tgaccaacca tggctcgctc tgctcgagcg cctgcaccga
     4501 accgccactg gcggagacca gtgtatggag ccgttccagc gtcgccttcg gcaggacttt
     4561 gctcaggctc tggccgtctt cgtacgccat gtatttctgc atgatcgccg ccgtttccgg
     4621 ggacgtcatc tcgcgagggt cgacttcgaa cagcagcgac tcggcatcat cgaatgcgcg
     4681 gtcgatctcc accggcatcg gatagtcgtc gctcttcagc aggtggaagg agcccagcag
     4741 gtagacgtcg ttgtcggcgt cggacacttt ccacagcagc gggcgcgcga cgaccgtggc
     4801 cgcccccgcg ttggcgatgg gggatggggc cgacttgttg gcctggtccg catgcgcggg
     4861 cagcgcgaac gcggcggcaa gcgccagggt gagcaatcgt ggggtcacgc gggatccttc
     4921 ggtggcggaa tggaggtgtc gtcgggtttt gcgtaggcct tctcgccggc cgacaccgcc
     4981 aggtccaacc ggttctccgg tggcggcagc gggcaggttg cgaacggggt gaatgcgcac
     5041 ggcgggttgt aggcctggtt gaagtcgatc agcacgcggc cctgcgcatc gggtttcggc
     5101 gcatcgatga agcgcccggc gccatagctg ccatgaccgc tggtgcggtc ggcgaacacc
     5161 aggaagagcg tgtcttcacc ctcgtccagc gcttccagcc ggtaggtctt gccgtcgcgc
     5221 tggaactcga gcgcgccggg attggggatg tggtcgatgg tgccgatgat gttggctatc
     5281 ggcagggtct tgcccgccgg atgcgcaatg aaacgcgcgg gcacctgcca gtcgcggcca
     5341 ccgggccagt attccaggcc ggcgaaatgc acccggctcg gcgcgtcggc gtgcttgacc
     5401 cgcagggcga gccgaccacc gcgttcgatc acggtgacca gccccttgcc ttcgtcgaac
     5461 gcgatcacgc tcggtcccgc tgggtccttg tcgctgcgca acaccccgcc gcgcgccggc
     5521 ttgccatcga ccgtgaccgg cacatcggcc acgaaactcg ccttgccgtc gcggacggtg
     5581 aagacgccca ggtgctccgg gcccatcgcc aagcggatgc cgttgtcgac atcgcggccc
     5641 acgcggtgcg cgccgggatc gagccaatgc atgccgacca ggctggtcca gccatccgcg
     5701 cggctgaggt cgatgacccg ccgtccacgc cacatgaact ggtcgtgcgc gtactgcttg
     5761 agcgcggctt gcagttgggc cgcctcgtcc ggggtggcct gcggcggtgg cgcgcgctcg
     5821 cagcccgcca tcgccagcac gcaggccagc gcgatgcccg cgcttttcat cgtccccgca
     5881 ggaaccatcg atccatctcc gccaggccga aacgcgtcca cgtgggacgc ccatggttgc
     5941 actgccccga acgttcggtc gcttccatct cgcgcagcag ggcattcatt tcatgcaggg
     6001 tgagccgtcg gttggcgcgg accgcgccat ggcaggccat cgtggacagc agttcgtcgc
     6061 gggtctcggc cacgcgccgg gtggtgccgt gttcgcgcag gtcggtgagc acgtcgcgca
     6121 ggagggcttc cacgtcgccg tgcgcaagca atgcaggcac gctgcgcagc agcagcgatt
     6181 gcgggccact gcgctgcacc tcgaagccga gtcgcgcgag ggtgcctgcc tcacgctcgg
     6241 cgacgtcggc ctcgcgttcg gacacggcca ggctggacgg caccagcaac ggctgggtgc
     6301 gcaggccggc gccgtcgtgc gcggctttca gtttttcgta accgatgcgt tcgtgcgcgg
     6361 cgtgcatgtc gacgacgacc aggccatcgg cgttctcggc caggatgtag atgccgtgca
     6421 actgcgcgac cgcgaagccg agtggcggca tgccgccggg cgcatcgtcg ggcatcggca
     6481 tcgcggcaac ggtggcgggc gattccgcgg cgtccgcaaa cgacccgcca tcgcgcggcg
     6541 cgtacagcgc ggcataggcg ctcggcgcgt ccgccacgcg caagccgatc gacgattgct
     6601 gcggcgccgg catcgaccat gcatgcggtg cctgcatcgg ctgcatcgcc gtggctgcgc
     6661 cgcctgcacg cgtctcggcc agcgcggcat gcagcgtgcg gtagacgaag tcgtggacca
     6721 gccgcgattc gcggaaccgc acttcgtgct tggccgggtg cacgttgacg tccaccccgc
     6781 gcggatcgag ttccaggaac agcacatagg ccgggtgccg gccatggaag agcacgtcgg
     6841 cgtaggcctg gcggatcgcg tgcgacacgt tgcggtcgcg cacgctgcgg ccattgacgt
     6901 acaggtattg ctggtcggcg ctcgccctgt tgtaggcggg ctgcgcaatc cagccgtgca
     6961 ggcgcatgcc ggcgccggcg tggtccacgc gcagggcatt gcgggtgaag tcctcgccca
     7021 gcgcctcgtg caggcgcacg tcgctgagca tgctgccttc ctgcaactgc gcctcgccct
     7081 tccagcgccg cgaggccttg ccgttatggg caacgcgcaa ttcgacatcg ggccgcgcca
     7141 gcgccagcgt gcgcagccag tcctcgatat gcgacagctc ggtgcgttcg gcgcgcagga
     7201 acttgcgccg cgccggcacg ttgtagaaca ggtcgcgcac ttccaccgtg gtgccgggcg
     7261 gatgcgcgtg cggcacgacc tcgccgacct tgccgccctc gacctgcagc agcgcgccgt
     7321 gttcgttgcc gttgcgccgc gacgtcaacc ggaagcgact caccgaggcc accgagggca
     7381 gcgcctcgcc gcggaagccg agtgtcgcga ccgcctccag gtcatcgatg ctggcgatct
     7441 tgctggtcgc gtggcgctgc acggccagcg gcaattcggc ggggtcgatg ccgccgccat
     7501 cgtcgcggat ccggatcagg cggatgccgc cgtcctccag gtcgatgtcg atgcggcgcg
     7561 cgccggcatc cagagcgttc tcgaccagct ccttcaccac ggacgcgggc cgttccacga
     7621 cttcgccggc ggcaatctgg ttgatgaggg tgtcgggaag ctggcggatg gtcacgccgg
     7681 gattttagcc ggcatggcca tcaattcagc agaaaacctt ggctcgtcat tcccgcgaag
     7741 gtgcgaatcc agcgtctttc atcgccaagc acatcggaag agcaggaaac gtcagtggat
     7801 tcccgccttc gcggggatga cgagacggac ttacggactg ccgcccgcgc tcgcaccagc
     7861 gcccgaggtg gacgcctggc tgacctgggt cgcctgggcc cgcgccgcat acaaggtgcc
     7921 cgggggcggc tggcgggtga agtactggtc gatcccggcc agcaccgctg ccgccagctt
     7981 gcgctggtag tcggggctgg acagcagggc ctcctccccg tggttggaga tgaagccggt
     8041 ttccaccagc atcgccggca cgtcggaggt gcgcagcacc gcgaagttgg catgttccac
     8101 gcggcgggtc ttgcccacgc tgcccaggct ccccaagaca tgcgcggcgg catcggacga
     8161 ggccttcatc tgcccgctct gggtcaggtc cagcagcacc gatttcaggg tgtcgccggt
     8221 ggcggccagc ttcacgccac cgatcaggtc cgcggcgttt tccttgtcgg ccagccagcg
     8281 cgcacgctgc gaggaggcac cgcgcgtcga catcacgtac accgaggaac ccgtggcatc
     8341 gcgattctcg gcggcatcgg cgtggatcga cacgaagatg tcggcgcggg cgccccgcgc
     8401 cagcaccgcg cggcgattga gcgggatgaa gacgtcggta tcgcgggtca ggtgggcctt
     8461 catgccgggc gtggcattga tctggcgggc cagttcgcgg gcgatggcca gggtcacgtt
     8521 cttctcgcga ttgccgttgg ggccgatcgc gccgggatcc tggccgccgt ggccggcgtc
     8581 gatggagatg accagcgggc gcatgccggc ctgcatcgcc gcgcgcgtga cgggcgcggc
     8641 aaccgtggcc gtctcgctgg cgatcggctc cggcttgccg gtggcgatgc gggtcggcac
     8701 gccggtggcg acggtgccgg gcaactcgga gaccgtggcg gccttgccca cgagcttgcc
     8761 gggatccgtt tcgacggcag cggaggactt ggcgatctcg gcgatcggat cgggcctggc
     8821 cgcggccacc ggtgccgcag gcaccacgac cgccgaggcg acgccatcac cgggccattc
     8881 cagcaccagc agcgggcctg ccgcggtggt ttcgatgcgc ggcggcattg ccttgacctg
     8941 gctggccagg tcgaacacca cgcggatcgt gccgggctgt ggctggccgg tgcgcacggc
     9001 cttcacggca cccgcgccgg caggcagcgc caggttgcgg gccagtcggc tgtcggggaa
     9061 gtccacgacc agacggtcgg ggttgcgcag ttcgatgacc ttgtagtcgc cgctgcgatc
     9121 gagctgcagt tcgacgcggg taccagtggc gccggcatcc acgcgcaact gcttgatttc
     9181 cgaggcgttt gcgaggttcc agcacagcgc cgccagcagc atcagcgcca gcaggacttg
     9241 ctcgaggcgg atccccttca ctcgcatgcc cggaagttaa gcacttgcca ttcccaagcg
     9301 caagcacttt tcccttaata atccgtggcc tgccgcactt tcctaaatgc gcgcgcaact
     9361 cctgcccgga gcgtcaagca gatcctagcg cctgcagcca ggcctcgccg cctggcgtgc
     9421 gtggctgcag atggcagcga cgccccgtcc cttccaccgc cagcaccaca tcgaggtcag
     9481 ccgcgggcag gccaccctgc ccgcgctccg gccattcgac cagccacagg cgcgtctcgg
     9541 cgggatccag ccccaggaac tccagctcgc cgggatcgcc gatccggtac aggtccaggt
     9601 gcagggccac cccgccgttg ggcaacgggt agtgctcgac cagggtgtag gtcgggctgc
     9661 gaatggtgcc ggtcacgccc aacgcacgca gcaacgcccg ggcaagcgtg gattttccgg
     9721 cgccgaggtc gccatgcagg taggccacgg catgtggtgg cagggcgctg gcgaggcgcg
     9781 cgccaagcgc atccgtggcg tccgcgtttt caaggaagac atcgtgcatg cgcctatcgt
     9841 cgcatcggat tggccagccg gcgcaaccat ggcatgaggt ccgagggcag caggccgcgc
     9901 tcgccgtcct cgcgcgccgc tgcatcacct gccgctgcat gcagcagcgc gccggccgag
     9961 gcggcatcgg ccgaggtcat gccttgcgcc agcagggcgg cgatgacgcc ggtgagcacg
    10021 tcgcccatcc cgccgaccgc catgcccgga ttgccggcgc agatcaccgt cgacggcgca
    10081 tttgcggagg cgatgatgct gcccgcgccc ttcaacacca ccacgcaccc aagccgttcc
    10141 tgcagcgctc gtgcggcggc gaagcgatcg cgctgcacct cgcttgtcgg gataccgagc
    10201 aggcgcgcgg cctcgccggg atgcggtgtt gccacatcct gcgcacgcag gcggtgcccc
    10261 gatgaagcga acaggttgag cgcatcggca tcgaccacgc gcggcgcttc actggccagc
    10321 acggcctcca gcagctgtcg tccccactcg ccctgcccca acccggggcc gagtgcaatg
    10381 acgccggcgc gtgccagcaa gggcgcgagt tgcgcaacgt cgtcgaccgc gtgcgccatc
    10441 gcctccgggc ggcgtgccag caacgcaggg acatgcgacg cgcgcgtcgc cacgctcagc
    10501 aggccggccc cactgcgcag cgccgcctcc gccgccagca ggatcgcgcc accactgccg
    10561 tggtcgccac cgatgcacag cacatgcccg ttggtgccct tgtggctgtc gcgcggacgc
    10621 agcgaaaaga accccggtag cgcgtccgcc cgcaatgcca acgccgcggg ctcgatcccc
    10681 tcgaacacgg tttccggcag gtcgagcgcg gccagtgcga ggtcgccggc atggtccagc
    10741 gcgatgccgg tgcgcaggcc gcgatgccgc gcgatgaatt gcagggtgcg cgctgcaatg
    10801 acggccacgc ccggcgcatc gccgctgcgc gcatcgatgc cactcggtac atccagcgag
    10861 aacacggggg ccggatgccg gttgatcgcc tcgatcaagg cgttggcatg cgcatccggt
    10921 gcgcgcgaca ggccgatccc gaagaccgcg tcgaccaaca cgtcggcacc gccgagatcg
    10981 ccatcgaagc actcgacctg ccctcctgct ccgacgaagg cgtcatgcgc ctgcatcgcc
    11041 agcggcgtgc gcggcgcgtg cgcgtcgagc cgcagcaccc gcgcgtcgag gccggacgcc
    11101 aacgcatgcc gcgccagcac atagccatcg ccgccgttgt tgcccggccc gcaggccacg
    11161 accagcctcc gcgcatgggg ccaatgcttc agcagctgct gccaggccgc caggccggca
    11221 cgcgccatca acacgaagcc atcaccgcca caggccgcga tgccgcgcgc ctcgagctcg
    11281 cgcaactggt cgggggcgta cagcgggaac ggactgtgca tcggccgatt ctagaaccga
    11341 ctcctatact tcgcccatgt cttcgccgct ccccgactac atcgcactcg cggaccgcat
    11401 ccgcgcgctg gcgctggcag cgggcttcca gcgcgtgggg atctccgggg tggaattgca
    11461 ggaggacgaa acgttcctgc agtcatggct ggcggaaggc ctgcacggtt cgatggcgtg
    11521 gatggccagc cacggcgaca agcgcagccg gcccgccgaa ctgatccccg gcacgctgcg
    11581 ggtggtgtcg gtgggcatgg attacggcaa ggacacagac gcggcctggg acaacctgcg
    11641 cgatggcgaa cgcgcctacg tggcccgcta cgcgctgggc cgcgattacc acaagctgat
    11701 gcgcaaccgc ctgcagcggc tcgcggacga cgtggcgaag gagatcggcc cgttcgggca
    11761 tcgcgtgttc gtagattcgg cgccggtgct ggaacgcgcg ctggcgcgca acgccgggct
    11821 tggctggatc ggcaagcaca cctgcctgat cgacagggac ggcggcagct ggttcttcct
    11881 cggcgaactc ttcgtcgacc tgccgctgcc gatcgacccg ccggcgtccg cgcactgcgg
    11941 cagctgcacg cgctgcatcg aggtctgccc gacccaggcg atcgtcgcgc cgtaccgcct
    12001 ggatgcacgt cgctgcatca gctacctcac catcgaacac gaaggcagca tcgacgaagc
    12061 cctgcggccg ctgatcggca accgcatctt cggctgcgac gactgccagc tggtgtgccc
    12121 gtggaacaag ttcgcgatgc gcagcgacga accggatttc cgcgtccgca acgacctcga
    12181 ccgggccacg ctggtcgact tgttcgcgtg ggaggaagac gaattcctgc gccgcaccga
    12241 gggcagcgcg atccgtcgca gcggccacga gcgctggctg cgcaacatcg cggtggcgct
    12301 ggggaatgca ccgaagacgc ccctcgtcat ggcagcgctc gaatcacgtc gtaagcatgc
    12361 atcgcccgtc gtgcgcgagc atgtggaatg ggcgctgcgc caacacactg cgaacgaagc
    12421 gccatgacca tcgatgggcc ggctggcccc gattcacttc gggacacgcc gtgaatacat
    12481 ccatgtaggc tcatcggcga catccatgtc gccgaaggtc ccgaagcaaa ccgggaccat
    12541 ccgtcctgcc gatcagcctc gttgactgaa ctcccgcaac aacgcgcgcg tcaccgcatc
    12601 gtcggcttcg gcatcgcctt gcaaggccgg cagcagccgg ctggcgacct gcttgccgag
    12661 ctccacgccg aactggtcga aggcattgat gccccacagc accgactgca ggtagacgct
    12721 gtgctcgtac agcgcgacca gcatgccgac ggcatgcggc gtcaccgcgt ccagcagcag
    12781 caaggtgctg ggacggttgc cggggtaacg ccgatgcgga tcgtcgctgt cctgcccgtt
    12841 cgccagcgcc tcggtctgcg ccagcaggtt ggccagcagc gcggcgtggt tctgcgcgtg
    12901 cggatggtcg gcccgcagca cgccgatgaa atcggccggc acgatttgcg tgccctggtg
    12961 cagcgcctgg aagaagctgt gctgggtgtc ggtgcccgcg ccgccccacc acaccggcac
    13021 ggtgtcgaca tcgaccgcgc cgcccgcgat ccgcaccgac ttgcccaggc tttccatcac
    13081 cagttgctgc aggtaggccg gcagcaattt caggcgatcg tcgtaaggca ggaccgcctg
    13141 cgtggccgca tgcaatgcgt tgcgattcca cactgcggtg agcgcatgcc acaccgcgat
    13201 gttcccgcga ggcgcggccg tggccgcatg cgcgtccatc gctgcagcgc cgtcgagcaa
    13261 ctcgaggaag cgatccatgc ccagcgccag cgcgatcggg aagcccactg ccgaccacag
    13321 cgaatagcga ccgcccaccc agtcccacat cggcaaggtg cgctccggcg gcacgccgaa
    13381 cgcagccacg cgctcggcat tggtgctgac ggcgtacagg cgttcgtcgt tgcccagcca
    13441 ggcgcggacg atgccgccgt tgagcagggt ttcctgggtg ccgaacgtct tggagatgac
    13501 gatggcggca ctgcgcttcg ggtccagtcc ggccagcacg cgctgcgcgg cattgccatc
    13561 gacattcgag atgaaatgca ccttgaagcg cgcatcgtgc agggccagcg cgtcgaccat
    13621 cagccgtggc cccaggtcgg aaccaccgat gccgacgctg acgatgtcgg tcacgtcgct
    13681 ggcttcgagt tgttcgatca gcacgcgcat ccgcgcttgg gcggccacgg cctgcgcacg
    13741 cgcctcgacg gcgacctgcg aggtcgaatt gtcgccgcgc aaggcactgt gcaacgcggc
    13801 gcggccttcg gtcggattga cgagggcgcc atccagcagg cggcgcatgg cgaccatcaa
    13861 ttccgcgcgt tcgccaatgg cgaacaacgc gtccagcgcg gcacggccgt agcgctggcg
    13921 ggcgaagttc gcgtagaggc caccgcagcg cagcgcgaaa tcgctggcac gggccggatc
    13981 ctcccggatc aggtcggtga tggtcgtgct ggccagcctg cgtgcttccg cgtcgagtcc
    14041 ggccagcatg ctcacgcggc ctgcttcggc atcgaccggt tggtgtgggt cagtgcgttg
    14101 tcgcggtcca catagaccag ggtcggcttg aacttggcgg cttcggcttc gtcgcactgg
    14161 ccgtaggcgc agatgatgac caggtcgccg ggctgcgcct tgtgcgcggc cgcgccgttg
    14221 accgagatca cgccgctgcc ttcctcgcca cggatcgcat aggtcgcaaa gcgctcgccg
    14281 ttgttgacgt tgtagatctc gatgcgctcg tattcgcgga tgccggagat gtccagcagg
    14341 cggccgtcga tcgcgcagga gccttcgtaa tgcagctcgg catgggtgac gctggcgcgg
    14401 tggatcttgg ccttcagcag ggtcagttgc atgttcgact ccagtggcgc gaaacccggt
    14461 acgggcccgg gaggccgaag tataggctgc gatttccgcg ctgcaacaaa cccgccgcgc
    14521 gccccaccga tgccggcttc agccggccag gtggaactcg aggttgtcga tcaggcgggt
    14581 gcgaccgagc cgggcggcga tcagggcgac caggtcgccc gcctcgccat ccatcggctc
    14641 ggtcaggtcc gggcggcgca ccaccgcgta gtcggggacg aaaccggcat cgcgcagggc
    14701 ctcgtcggca tcggcctcga cctgggcgag cggcaggccg accagcaggc cgtcgcgcat
    14761 cgcgtgcagg gtgtggtgga tgatcgccga cacctcgcgt tcctgcggcg agaggtactg
    14821 gttgcgcgaa ctcatggcca gtccgttcgc ttcgcggacg atgtcgccgc cgacgatgtc
    14881 gatcgggaca tgcaggtcgg cgaccatctg ccggatcacc gccagttgct ggtaatcctt
    14941 cttgccgaac accgccacat ccggctgcac ctggttgaac agccggctga ccacggtgca
    15001 cacgccgtcg aaatggcccg gccgatgcgc gccttccagc accgcgctga cgccggggac
    15061 gtgcacgctg gccgccaggt ccacgccgaa cggatacatc gccgccacgt ccggtagcca
    15121 cagcacgtcg cagccggcgg actgcaggcc gctggtgtcg gcatccgggg tgcgcggata
    15181 gcgggtgaag tcctcgttcg ggccgaactg ggtcggattg acgaagacgc tggagaccac
    15241 cttgtcggcg tgctggcggg ccagcgcgac cagcgaatag tggccggcgt gcaggttgcc
    15301 catggtcggc acgaagccga cgcgcaggcc gtcgcgcttc cagccgcgga tgcggctgcg
    15361 cagcgattca agcgcggtga tggtctcgat catgtgggtt cgctcggcaa tggcgtcagg
    15421 cgtaggcgtg ttcggccgcg gggaaacggc cgtcgcgcac ggcatcggca tacgcacgga
    15481 tcgcagcggc gacggagccg ccctcggcca ggaaatcctt gacgaatttc ggcttgcgat
    15541 ggccggtgtc caggcccagc aggtcgtgca gcaccaacac ctggccatcg caacccgcgc
    15601 ccgcgccgat gccgatggtc gggatcgggc tggcggcagt gatctgcgcg gccagcgcgg
    15661 ccggcacgcc ttccagcacc agcagcgccg cgccggcgtc ggccacggcg agcgcgtcct
    15721 cgcgcaattt cgcggcggcg gcatcctcgc gtccctgcac cttgaacccg ccgaagcgat
    15781 gcaccgattg cggggtcagg cccaggtgcg cacatacggg aatttcgcgc tccacgagat
    15841 agcggatcgt ctcgagtttg tggcccgcgc cttccagctt gaccatgctc gcgcccgcct
    15901 ggatgaagcg caccgcggcg gcgtgcgcct cctgcggcga gccgtccgaa ccgaacggga
    15961 aatccgcgat tttcagcgcg cgtgtcgccc cgcgggccac cgcggcggtg tggtagacga
    16021 tgtcgtccac gcgcaccggc agggtggaat cgaagccctg caccaccatc cccagcgaat
    16081 cgccgaccag gatgaggtcg atgccgttgt cgtcgagcac gcgcgcgaaa ctggcgtcgt
    16141 aactcgtcag catcaccaat ttgcggccgg cggcacgcgc ctcgcccagc atcggcacgg
    16201 tccagcgttt ctgtggctcg ctcatggccg gctccttccc gcgggcgcct aaggtaacgg
    16261 ttcgatgccg tccgccgcca tcacgcccgc acactcacgc gcgggcccga tgccgggaat
    16321 gcctgcgtcc ggccaggcct ccagcagcgg cagcaaggcg aacgcgcgtt cgtgcaggtg
    16381 cggatgcggc aggcgcaggc cgggctcgtc gatgatggca tcgccgtgga gcagcaggtc
    16441 gaggtcgagg atgcgcggcc cccagcgttc gccttcgatg cgaacccggc cggcatcacg
    16501 ctcgatggca agcaggacat cgagcaggtc gcgcgccgac agcgtcgtgt cgaccacggc
    16561 gacggcgttg acgaaatcgg gttggtcggt cacgccccag gccggcgtgc gatagaggcg
    16621 cgaggcgcgc agcaggcgcg tgcctgccac gttgtcgagc gcatcgatgg catcgcgcag
    16681 cgtgcgcgcg gcgttaccca ggttggcacc caggcccacc gccgccaggg tcatgcgggc
    16741 tcttgcgagg cagcgcccgt cgcgtcggct ccgccgcgac gccgacggcg acgccgcttg
    16801 cgcggtgcat cgccctcgcc gcctgcatcg gcggccgcgt catcgaaccc gtccgcaccg
    16861 gcgtcgagtg cgccctgctc gcccgcggcc acggtcgccg atgggtgcag ttgcgcctcg
    16921 cgccagtagg ccacgtcctc ggcatgcgaa tccgaggcgg ccaggcgcag ctccaggaaa
    16981 tcgaacgcgg cgcggaagcg cggatgcgcc agcagccgga acacgcgctt gcgcacgcgc
    17041 tgcgagaagc gcggctgcag cagccagatt tcctgcatgg gcagcgagaa ccggcgcggc
    17101 aacgcggtgc gcccgacctg gtgcaacgtg acccggtcgg cggcgcggcg ctgcgcgtcc
    17161 gcggcatgca cgccctgcgc ctgcagcaat gccagtgcgc ggcaataggc cggccacaac
    17221 agcacggcat acaggaatgc aggcgatacc gattcgtcgg cggccacgcg cgcatcggta
    17281 ttgcgcagtc cctgcaccag catccggcgc aacgcgccgg acttgttcga tgccagcgcc
    17341 tgggcggttt ccggcagcaa tgccggcagc aggccgtgcg cttccagttg ctcgaagctg
    17401 gccaccgcgt gccccgacag gaacatcttg agggtctcct cgaacagtcg cgccggcgcg
    17461 gcctcggcca gcagcggcgc gagccgcgcg atcggctctg cggtcgccgc ttcgatgctg
    17521 aaatcgagct tcgccgccag ccgcgccgcg cgcagcatcc gcaccggatc ctcgcggtag
    17581 cgggcctcgg gatcgccgat caggcgcagc aggcgcgcct gcacgtcgtc gtaaccgccg
    17641 acgtaatcgc gcaccgagaa atcctcgatc gcgtagtaca gcgcattcgc ggtgaagtcg
    17701 cggcggatcg catcgtcctc gatcgtgccg tacacgttgt cgcgcaacag gcgaccacct
    17761 tcatgctgct cgcggtcgcc actgccgtcg tcgatgttgg cgcggaaagt cgcgacctcg
    17821 atgatctcgc ggccgtagac cacgtgggcc aggcggaagc ggcgcccgat cagccggcag
    17881 ttgcggaaca gcgacttgac ctgctccggc gtggcgtcgg tggccacgtc gtaatccttc
    17941 ggtgcgccgc cgaccagcag gtcgcgtacc gcgccgccga ccaggtagcc gccgaagccg
    18001 gcttcgcgca ggcggtacag cacgcgcaag gcgttctggc tcatctgctt gcgcgacagg
    18061 ccgtgctcgt cacgggcgat cacgcgcagg ctggggacgg gatagagtgg ctgggattcg
    18121 atttcggtca tgcgctacgg cggagccgcg ctctggaatg ggatgggttg cgttgccgtc
    18181 ggcaccgcag ccgcatatac tagcaagctc gacacgcggc cctgccgctg gcgagccggt
    18241 cgtccagccg gcgagttcca ccccacctct gctcccatcg tctagaggcc taggacgccg
    18301 ccctctcaag gcagcaacac gggttcgaat cccgttggga gtaccaatca acaaaaaagc
    18361 ccgagtcatt cgactcgggc ttttttgttg attcgcccta cgggatgaga acccgtacgg
    18421 gttcgacagc ggcgcccgga gcgcggctgg acaaccgaag gttgcccgca gcgaagcgaa
    18481 gggcgcggca caaggatgtg ccgcgtctat cccgttggtc catcatccat cccgttggca
    18541 cgctcacgac gtggagcgcc atggccacac aaccgccacc cattccgatg cgccgcctcc
    18601 cgccgccacg gcgctaaact acccgcatcg catcacggat tgtccccatg cccttcgtcg
    18661 tcaccgagaa ctgcatcaag tgcaagcaca ccgactgcgt cgaggtgtgc ccggtggatt
    18721 gcttccacga gggcccgaac ttcctggtga tcgatccgga cgagtgcatc gactgcaccc
    18781 tgtgcgagcc ggaatgcccg gtcaacgcga tctacccgga agaagacgtg cctgccggac
    18841 aggaagtctt cgtcgcgctc aacgcggaac tggcgaagga atggccggtg ctggcgaccc
    18901 gcaaggatgc gccggccgat gccgccgagt gggatggcaa gccgggcaag ctggccctgc
    18961 tggagcgctg atccgccaca aatcgccaac aagaagggcg ccttgcggcg cccttcttgc
    19021 gttgcagagt tcacatgtca gggcgcgatg gccgccttca atgcaccgat cagcttgacc
    19081 ggcgcctcgc cgatcggcgg caggccgcgc gggtcgaccg cggagatgta cacgccctcg
    19141 ccggccttgg tcacccggac caggaacttg gcgtccatgt aatcgacgtc gtaggtaccc
    19201 aggatctgcg ccttgttggc aatcgtcacg ccgggggttc ccgccaggac ctcgccgacc
    19261 ttggcgaaca gcgtgtcgcg gtcgccgctg gcattgaagc cgatcggcgc cgcgctgctg
    19321 ccaccggcgt tgttcatgcc cgaggcgctg acgctgccac ccgccgccgg cagcgacatg
    19381 gccttgtcgg cactcggacg atccaggtcc ggcgggacct ccagcgggcg ggtttcggcg
    19441 ctctgcgtgt acagctcgtt cttcttgccg aaccagtggc aaccggacag gccggccacc
    19501 agcagggcgg cggcgagcgg tgcggcaatc ggggacacgc gggcactacg acgcatctgg
    19561 agctcctgtc aggccgctgc gggcggcatc agtcttcgag ttggaggatg tccgcatgca
    19621 ggcgctgggc ctcggcggca tgcgtggatg atagcggctg gagcggcagg cgcaggccat
    19681 ggccgattcc gttcagcgcg agtagggctt tgacggggat cggattgggc tcgacgccga
    19741 ggaagtggta gagcgcgtac aaccttgcat ccagcgcctc ggcgccggcc acgtcgccgc
    19801 cgcgggccag ctggcacaag cggcggaagg tgccgggcac gacgttcgac gcgaccgaga
    19861 tcaccccgtc cgcgccagcc agcatcgccc gcaccgcggt ggggtcgtcg ccactgagca
    19921 cggcgaagcc gtcgcgccgg caaggcaaca ggtcctccat gcgcgcggca tcggcacggg
    19981 cttccttgat gccgacgatg ttcggatgca gggacagttc gccggtggtc tccggggcca
    20041 ggtcgcagcc ggtgcggcct ggcacgttgt agaggaccac cggcaacccg ccctcgtcgg
    20101 cgaccgcgcg gaaatgcgcc agcaaccccg actgggtggg ccgcacgtag ggcggcgtca
    20161 ccaccagcgc ggcatcggcg cccagtgcgg cggcacggcg gttggccagg atggtcttgg
    20221 cggtattgga atgaccggtg ccggccagca ccggcatgcg cccgccgagg aattccaccg
    20281 cgctggccag cagcgcatcg tattcgtcat cgaatagcgc cgcggcctcg ccggtggagc
    20341 cggccacgac cacgccctgg gtgcccgcgt cccgctgcgc ctgcagcaac cgctgccagg
    20401 cggggaagtc gatgtcgccg gcggccgtga acggcgtcgc cagtgcggtg atgctgccgg
    20461 aaagtcgcac gtgggggctc gggctgggcg cggcgaagga acgcgcggcg cgatgggatg
    20521 ggtgcagcga agctgaatct tacttgcggc tcgaaaccac cggcaagtat gctgccgcat
    20581 gcgtcgcatc cctcccgcgc cgccgacccc acccgcttcg gacgctccct tgaccgatat
    20641 ctccgcccgg ccagcgccga acgaaaacca cctgctgatc aacgcgtaca ccacgcatcc
    20701 gcagtcgccg ctgctggccg tcacccggcg catctccgat tccggctgca acctggtcga
    20761 cacgcgcctg gccacggtgg gccgcgatgt ctcggtgacc gcgctcgcca ccggctcgtg
    20821 ggacgcggtg gccaagctcg agaccatgct cgccaagctg gagcgcgagg aagggctgaa
    20881 gctgatctgg taccgcaccg ggcccaagcc ggtgcagtcc agcctgctgc cgtacgtggt
    20941 cgaggtgatc gcctcggaca agccgggcat cctgttccag ctggccgact tcttcgacca
    21001 gcagggcatc accatcgaga gcctgcattg ctcgcgctac cgggcgatgc agaccggggc
    21061 cgacatgttc tccgcgcagc tgaccatcgg cgtgccgtcg gacatgcata tcgccgcgtt
    21121 gcgcgacgac ttcctcgagt tctgcgacca cctgaacctc gacgccatca tggatccgat
    21181 gaagtactga cacaggggat cgaagaccat ggaattcgtg ctgttcttcc tcgccgtggc
    21241 gatgatcgca ggccaacacg gcgaaatctc gaaactgaag aagcgcatcg acgcgctgga
    21301 aaccagcccg cccgcagccg ccgacacccc atgatcgccg gggcggccct gcatggcgcc
    21361 gtgtcgaggc tgccgctggc gctttccgac ggcacatccg cgacgctcgc cgattatcgc
    21421 gggaagtggc tggtgctcta cttctaccct agggactcca ccccggcctg caccaccgaa
    21481 ggcctggatt tcaacgcgct gctgccggcg ttcaaacgtg ccggcgccgt ggtgctgggc
    21541 gtgtcgaagg actcgctgaa gtcgcaccag aacttctgcg cgaagcaggg cttcaagttc
    21601 gacctggtca gcgatgccga cgaagccgtc tgcaacgcct tcggcgtgat ccagccgaag
    21661 aaactctacg gccgcgagta catcggcatc gtgcgcagca cctacctcat cgaccctgaa
    21721 ggcgtcatcc gccaggcatg gcaaccggtg aaagtccccg gccacgcgca agccgtgctt
    21781 gacgcgttga aggccgcaaa gcgccagtga ccatccccat cgccacccgc ccccgcccgg
    21841 cgcagcgcgc cgggcgttca tcgtcgcgcg cgccaatggc gcgcaagcgc tcctcttcac
    21901 cccatcgttg tccgactgca ggaatccgag catgacgcga agcaagcgca tctacgtcct
    21961 cgacaccaac gtgctgatgc atgacccgac cgcgctgttc aagttcgagg aacacgatgt
    22021 gtacctgccg atgcaggtga tggaagaact ggacaacggc aagaagggca ccagcgaagc
    22081 cagccgcaac gcgcgccagg tcagccgctt cctcaatgag ctgatcgagg cccacggcag
    22141 cagcgacgtc cacaacggca tcgctttggt gcgtccgcag gcgctgcagc tgcgcggcgc
    22201 ggaaagcgcc gggcgcctgc tgttccagac cggcgatttc gatgcgggca agcgcttcgg
    22261 cacggtgatc ccggacaaca acatcctcgg cgcgatcctg gcgctgaagg agtccgatcc
    22321 gggcgcgccg gtggtgttcg tgtccaagga catcaacctg cggatcaagg cgtcgatcgc
    22381 cggcatcgtc agcgaggatt acgaaaacga ccgcgcgctg gacgatttca gcctgctgta
    22441 cacgggcgca accgcgttgc ccgaggattt ctggtcccgc catggcaagg acctgaagtc
    22501 gtggaccgac aagggtcgca cctactacga aatcgcacgc ggcgaggacg aggactggta
    22561 tccgaaccag ttcgtctacc tgcccggcga cgaacaggcc gagatgaagg tggcgaaggt
    22621 ggccgacgac aaggtggtgc tgcagatcgt cgacgacttc cggcatgcgt cgcgcgcggt
    22681 ctggggcatc accgcgcgca accgcgagca gaacttcgcg ctcaacgcgc tgatggaccc
    22741 ggagatcgac ttcgtcagcc tgctcggcac cgccggtacc ggcaagacgc tgctggcgct
    22801 ggccgcgggc ctggcgcaga ccatggatgc gcagcgctat cgcgagatca tcatgacccg
    22861 cgccacggtc agcgtgggcg aggacatcgg cttcctgccc ggcaccgagg aggaaaagat
    22921 gacgccatgg atgggcgcgc tgaccgacaa cctggaagtg ctgacgcaca accaggacgg
    22981 cggcgcctgg ggacgcgcgg ccaccaacga cctgctggcg tcgcggatca agatccgctc
    23041 gatgaacttc atgcgcgggc ggaccttcct gtcgcgctac ctgatcctcg acgaggcgca
    23101 gaacctgacg cccaagcaga tgaagacact catcacccgt gccggcccgg gcaccaagat
    23161 catctgcctg ggcaatgtcg agcagatcga cacgccctac ctcaccgaaa ccacgtccgg
    23221 cctcacctac gcagtcgacc gcttcaaggc ctgggcgcac agcgcgcaca tcaccctgcg
    23281 ccgcggcgag cggtcgcggc tggcggatta cgcctcggaa gtgctctgac gatcctgcat
    23341 gaactaatgt ttcagatatc aagatgcata tcctgaaatt gcataagccc gtttgaaggt
    23401 tcaaaatggg gcttcctgac gggagcctcc catgatcagt tcagcccaga tgcgcgccgc
    23461 acgcgccctg ctcggcatcg accagaagca gctcgcgacg ctcgcgggcg tttccgtccc
    23521 tacgatccag cgcatggaaa gcagccaggg cacggtgcgc ggcgtggtcg gtacgctgac
    23581 caagatcatc gaggcgctgg atgccgccgg catcgaactg atcggcaacg accaccccag
    23641 ccagggccgt ggtcgcgggg tgcgactgaa ggagccgcag tgatggccgg gcgcatggac
    23701 gccgcgcggc gcgcctggta cggcatgttc gagccgaagc tgttgacggt cctgcgcgaa
    23761 ggctacggcc tgcaggcgtt caaggcggat gcgatcgccg gcctgaccgt ggcgatcgtc
    23821 gccctgccgc tggcgatggc actggcgatc gccagcggca ccaccccgga aaagggcctg
    23881 cataccgcga tcatcgccgg cttcctgatc tcgttcttcg gcggctcgcg ggtgcagatc
    23941 ggcggtccga ccgccgcctt catcccggtg gtcttcgtgg tcctgcagaa gttcggctac
    24001 ggcggcctga tcctgtgcac gctgttggcg gggctgatgc tgatcggcgc cggcctgctg
    24061 cggctcggca cgctcatgaa gtacatgccg cagccggtga tcaccggctt caccgccggc
    24121 atcgcggtga gcatcttctc cagccaggtc aaggacgtgc tgggcttgcg catggagacg
    24181 gtgcctgccg agttcctcgc gcgctggacg gcgtacggca agcacatcgc caccacccag
    24241 ccggcggcgg tcgggctgac cctcctcggc ctggtggtga tcgcgggcct gcgccgctgg
    24301 aagccgacct ggccgggctt cctgatcgca ttgctggcct gcacgctggc ctccgccgcc
    24361 ttcgccctgc cggcggagac catcggcacc cgcttcggga cgctgccatc cgccttgccg
    24421 acgttcgcga tcccgcacat cccgttcgag cgcaccttcg agctgctgcc cagcgcgttc
    24481 accatcgcgt tcctggccgg ggtggaatcg ctgctgtccg cggtggtcgc cgacggcatg
    24541 accggcgggc gccatcgctc gaatggcgaa ctggtcgcac agggcatcgc caatgtcggt
    24601 tccgcgctgt tcggcggcct gcccgcgacc ggcgcgcttg cgcgcacggc caccaacatc
    24661 cgcgccggcg gtcgcagccc ggtcgccggc atcctgcacg ccggctacct gctgctgttc
    24721 atgctcctgc tggcaccgct gatgcgccat gtcccgctgg cggcgctggc ggccgtgctg
    24781 ctggtggtgg cctggaacat gagcgagcag gaggccttcc gcaacacgct gtcggcgccc
    24841 aagggcgacc gcgtggtgct cttgctgacc ttcctgctga ccgtgttcat cgacctcacc
    24901 atcgccatcg aggtcgggat cgtggtggcc gcattcgtgt tcatgttccg gatggcggat
    24961 gcggtggaag tcagctccgc catcggccag ggcgacgctg gcgaccagcg ttcgcgcctg
    25021 ccggcggggg tcgaggtcta ccagatcagc gggccgctgt tcttcggcac cgcgaaccgg
    25081 ctggacaacc tgctggacca gttcctgtcc acgccgaagg tcttcatcct gcgcatgcgg
    25141 ctggtgcctg tcctggacgc aagcggcgtg cacgcgctgc agaaactggc cgaccgttgc
    25201 cagcggaagg gcatcgcgct ggtcatctcc ggcctgcagg agcagccgaa ccgcgtgctt
    25261 gcgcgcatgc agacgcatgc ccatcccgcc cacatgcacc tcgcgcccga cttcgaccac
    25321 gcattgaagc tggccgagcg cctggcccgc cagcctgcaa cggctccggc acaataggtg
    25381 gatgcagcac gaccgccccc ccagcgcact cagcatcgcc ggctccgatt ccggcggcgg
    25441 cgccggcatc caggccgacc tcaagacctt cgccgcgcat ggcgtgcatg gcgtgagcgc
    25501 gatcgccgcg ctgaccgcac agcacacccg tggcgtgacc gcggtgcacg tgcccgatgt
    25561 cggtttcctg cgcgcgcaac tcgatgcctg cttcgaggat ttcgacgtcg gcgcggtgaa
    25621 gatcgggatg ctcgcgaccg ccggggtcat cgactgcgtg gccgatgcgc tcgagcaata
    25681 ccgcccgcgg ttcatcgtgc tcgatccggt gatggtcgcc accagcggcg cgaagctgct
    25741 cgccgatgat gcgctgcacg ccttgcgcac gcggctgctg ccgctggcga gcatcgtcac
    25801 cccgaacatc cccgaggccg gattgctgac gggcctggcc atcgccgatg gcaacgacgc
    25861 cggcaacgcg atcgccgccc tgcgcgcgat gggtgcacgg gcggtgctgc tcaagggcgg
    25921 tcacctgcac gaaggcggca ccgtcatcga ccgctacgac gacggcgcgg tcgatgccga
    25981 gttcacccat gatcgccttg ccttggaagg ccacggcacc ggctgcacgc tggcctccgc
    26041 gatcgcggcg aacctgtgcc tgggcatgcc gatgccgctt gcggtcgaag ccgccaccga
    26101 ctacatcgaa cgcgccctgc gcggcggcta ccggccgggt cgcggcgacg tggtggtgct
    26161 cgaccacttc ggcgcggctg cgcgctgatg cgtgcggttg aactgccggt cgagaccggc
    26221 gatggccacc gctgcgaact gatcgcgcgt attccggagg caccgcgcgg gcgcgtgctg
    26281 tggctggccg gcatgggcat gccggcgcgg cattacctgc ccttcgccga tgcgctggcc
    26341 gcgcgcggga tggcggtgtt cctgcacgaa tggcgcggga tcggctccag caacgtgcgt
    26401 gcctcgagga cggacgactg gggttatcgc gaactgctgg ccgacctcgc cgccagccag
    26461 gcggtcgccg atgcgcaggt gcccggcctc cgtcgcgtcc tcggcgggca cagcctgggc
    26521 gggcagatgt cctgctgcca attgggactg catcccgaca gcgcggatgc gttgtggctg
    26581 gtcgcaagtg gtgcgccgta ctggcgcgtg ttcggcgcac ccaagcgctg gggcctgttg
    26641 tccgcctatc gcttcatgcg ctggctggcc gcacgcaatg gcttcatgcc gggccgccgg
    26701 atcggcttcg gcggcaacga ggcgcgcagc gtgatcgccg actgggcgcg caccgcgatc
    26761 agcggccatt acgccgcacc gggcgtcgat gtggacctgg aagcgggcat ggcgcgcttc
    26821 ggcggcgacg tccgtgcact gaccttcgac caggactggc tggcgccacc ggaatcaacc
    26881 gccttcctca ccggcaagtt ggcgaatgcg caagtgaccg cggggcactt cgacgccgcg
    26941 atggccggca ccgtggccga ccactacgcc tggatcaagt cgcccgacgc gagcgcggac
    27001 tggctgctgc gctgagcgct cagacccgcg cgaacaccag cgcggcgttg gtgccgccaa
    27061 agccgaagct gttcgacatc accgtgtcca gtttcgcatc gcgggtgtcg cgcacgatcg
    27121 ggaagccctc gcaacgcgga tccagcgtct cgatgttggc cgagccggcg atgaagccgt
    27181 cgcgcagcat cagcaggctg tagatcgctt cgtgcacgct cgccgcgccg agcgaatggc
    27241 cggacagcgc cttggtcgac gacagcggcg ggatcgcatc gccgaacacc tcgcgcaccg
    27301 cgttcaattc ggtgatgtcg ccgagcgggg tcgaggtgcc atgggtgttg aggtagtcga
    27361 tcgggcggtc gatgccggcc atcgccatgc gcatgcagcg caccgcgcct tcgccgctcg
    27421 gcgccaccat gtcggcgccg tcgctggtca cgccgtaccc gatcaactcc gcatggatat
    27481 gcgcgccgcg cgccttcgcg tgttcgtagt cttccagcac cagcatgccg ccgccgccgg
    27541 cgatgacgaa accgtcgcgg ttggcatcgt agggtcgcga tgcgttggcc ggcgtctcgt
    27601 tgaagctggt ggagagcgca cccatcgcgt cgaacatcac gctcatggtc cagtccaggt
    27661 cctcgccgcc gccggcgaac atgatgtcct gcgcgccgtg gcggatcagg tcggcggccg
    27721 cgccgatgca gtgcgcggag gtcgcgcacg cggcggacag ggaatagctc aggcccttga
    27781 tcttgaacgc ggtggcgagg ttcgcggaca ccgtggagca catcgtgcgc ggcaccatgt
    27841 acgggccgac cttgcgcacg ccgcgatcgc gcaacaggtc tgccgtctcc acctgccagc
    27901 ggctggaacc accgccggaa ccggcgatca ggccggtgcg cggatcgctg accgcagctt
    27961 cgtccaggcc cgcatcggcg atcgcgtcgc gcagcgagac atagctgtac gccgcggcat
    28021 cgctcatgaa acgcttgagc ttgcggtcga ccaccgcatc gaggtcgagc tcgaccgcgc
    28081 cgccgacctg gctgcgcaag ccgcgctcgg cgtgatcggg gaggaaacgg atgccgggcc
    28141 ggctctcgcg caacgccgag gacacggtcc caaggtcatt gcccaggcag gacgcaatgc
    28201 ccatgccggt caccaccacg cggcgcattg gcgtactcac atcgcctccg tcgactggaa
    28261 caggcccacg cgcatgtcgc gcgcggtgta gatctcgcgg ccgtccacgt aggtgcggcc
    28321 atcggcgacc acgaggcgca ggcgcccgcg cagcaggcga cggatgtcga tctcgtagct
    28381 gaccaccttg gcgtcgggca gcacctggcc ggtgaacttg atctcgccgg cgcccagcgc
    28441 gcgaccgcgg ccggtttcgc ccagccacgg caggaagaaa ccggtgagtt gccacatcgc
    28501 atccacgccc aggcagcccg gcatcaccgg atcgccgagg aaatgacagc cgaagaacca
    28561 caggtccgga tggatatcga gttcggcgcg gatgaagccc ttgccgtgcg caccgccgtc
    28621 atcgctgatg tgggtgatgc gatcgaacat gagcatcggc ggtgcgggca agcgggcatt
    28681 gccctcaccg aacaattcgc cgcgcgcaca ggccagcagc tgctcacgat cgagggagga
    28741 aagacgcgtc attgcagggc cctggacgac tagagcaacg attctaaagg gcagccgtgc
    28801 gacataccta tgcgtactgc gcacgggaat gatgtccgcc cgccatacct cggcgaacgt
    28861 tggaaacgaa accactgcga catccgcggc gcaggcgtat taagcttcag gttccccacg
    28921 ccggaacccg tccgtgacgc atgccgcgcc cgcttcgcta ttgaccatct tcggtgccac
    28981 cggcgacctc gcacggcgca tgttgctgcc ctcgctgtac agcctccagg cggagcaact
    29041 gctgccggcg aacctgcgca tcctcggcac cgcccgcagc gagctcgacc gcgacggctt
    29101 cgccaacctg gtgtcggcca gcattgccga gcgcatcccc gccgcggaac gcgacgatgc
    29161 cgcgttgcgc ggattgctcg accggctcga ctacgcggcg gcgagcgtgg acgacgatgc
    29221 gtcgatggcg gcattggcgt cgaaggttga agcgctgcgc gacggcgacg tgctctacca
    29281 catgagcacc gcgccgaagt tctacggccc ggcctgccag tcgctggccg cgcacggcgc
    29341 cgccggcgcg ggcacccggg tgatgctgga gaagccgatc ggcaaggacc tggcatctgc
    29401 gatcgccata aatgacagcg ttgccatggc cttcgacgag gagcgcatct accgcgtcga
    29461 tcactacctc ggcaaggaag gcgtgcagaa cctgatcgcg ctgcgcttcg gcaacgcgct
    29521 gttcgaaccg ctgtggaaca cgcgccacat cgagcaggtg cagatcaccg tcggcgagac
    29581 cgtcggcgtg gaaggccgcg gcgattacta cgacgactcc ggcgcgctgc gcgacatgct
    29641 gcagaaccac ctgctgcaac tgctgtgcct ggtggcgatg gagccgccgg cgaagttcga
    29701 tcccaccgcg gtgcgcaacg agaagatcaa ggtgctgcgc tcgctgcgcc cgatcggccg
    29761 cgagcaggtc gcccacgaat cggtcgccgg ccagtacacc gcgggcgcca gtgctggcgt
    29821 ggcggtgccc ggctacgccg acgaactcgg ccgcagcagc cgcaccgaaa ccttcgtcgc
    29881 cctccgcgcg cacgtggaca actggcgctg gtccggcgtg ccgttctacc tgcgcaccgg
    29941 caagcgcttg ccatcgcgca gcaccgagat ctacatccag ttccgcaagg tgccgtactc
    30001 gatcttcggc ggtcgcgccg ccggcgacat gcgcccgaac ggcctgctga tccgcctgca
    30061 gccggaggag cgcatcgagc tggacctgat ggcgaagacg ccgggcctcg accgcagcgg
    30121 cctgcgcctc tcgcaggtcg ggctggacct ggatttccac gaggagttcg ccaacgcgcg
    30181 caagcgcatc gcctacgagc gcctgtacct ggacgcgatc gaaggcaacg gcacgctgtt
    30241 cgtgcgccgc gacgaaaccg aggccgcgtg gcaatgggtc gatgcgatcg ccgagggctg
    30301 gcgcgccgcg ggcatcacgc cgaagccgta tccggccggc acctggggtc cgggcgcggc
    30361 agtctcgctg accgaacgcc acggccacgg ctggcgggaa tagccgcatg ccctggatcg
    30421 aacatgacca tgccgacgtg ggcgcattgg tggcgcacgt cgccgacgtg ttcgtcgagg
    30481 cctcgcgcga ggcgatccat gcgcgcgggc aggcctggct ggcgctcgcc ggcgggcgca
    30541 cgccgctgcc cgtgtatgcg cgacttgccg gggttggcct cggcggcgtg gtcagtgcga
    30601 tccccaccga tgaacgttgc gtcccgcacg accatccggc ctgcaacctg cgcgcattgc
    30661 gcgacgcctt cgccgccgat gccaacatcg tcgccaaccc actcacccgc gaggatggcg
    30721 atgtcgatgc gtcgctcgcg caagcacgtg cgctgctcgc ggtgaacccg caagccttcg
    30781 atgccgtgct gctgggcatg ggcgcggacg gccatttcgc ctcgctgttc cccggcgccg
    30841 ccaacctcgc cgaaggcatg gcgatcgact ccggcatcga cgccatcgcc accctgcccg
    30901 atccgttgcc gccggaagcc cccttcgcgc ggatcagcct gaccctgccg cgattgctgc
    30961 atgcgcgcgc gatccacctg gtcgtcaccg gcgcggacaa gcgggttgtg ttggggaacg
    31021 cgcaacacga tgcggcatcc aaattcccgg tggccgcgct gctgcatgcg cccggccccc
    31081 acgtccacat ccactggagt ccgtagcgat gcccctgcat cccgccatcg agcgcgtcac
    31141 ctcgcgcatc gccgaccgca gccggcgcct gcgtggcgac tacctggcgc ggatcgacgc
    31201 ggcgcgcgaa gccggcccgg cccgccgtga actgtcctgc ggcaacctcg cgcacggttt
    31261 cgccgcgtcc ggcgtcgaca agccgatgct gcgcgagggc gcgggcggca acatcggcat
    31321 catcacgtcc tacaacgaca tgctgtcggc gcaccagccg atggagcact accccacgct
    31381 gatccgcatg gccgcgcgca atgccggtgg cagcgcacag gtcgccggcg gcgtgccggc
    31441 gatgtgcga
//
LOCUS       DAOOCX010000013        29120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1269_length_29120_cov_8.321762, whole genome shotgun sequence.
ACCESSION   DAOOCX010000013 DAOOCX010000000
VERSION     DAOOCX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 29120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29120
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1269_length_29120_cov_8.321762"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PK399_02300"
     CDS             complement(<1..273)
                     /locus_tag="PK399_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HPW11991.1"
                     /translation="MRPTLPLLLCTGLLAGCGGGQALSPGSTEGVDPALPPPQRALIP
                     TVDIAPATGWPQGATPTPARGLVVSRFADGLEHPRWLHVLPNGDVLV"
     gene            complement(452..1399)
                     /locus_tag="PK399_02305"
     CDS             complement(452..1399)
                     /locus_tag="PK399_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11992.1"
                     /translation="MKRIACLVLLLSASLPCVAQQVADRDFAPAVSPPAFAEGKGPVV
                     CLDEAHHNFHTLDGRFRPFGALLRRDGYVVAPNGAAFDETSLSACAALVIANAQPSDA
                     DWNTYPTPTPSAFAPAEIAAVRDWVQGGGRLLLIADHMPLAGAASNLAAAFDVEFTDG
                     FAFAGVNDEAKAKPTLFRLDEGTLADHPILRGRHTGEAITQLRSFTGQAFRAPGAQPL
                     MVLPAGFVSLHPAKAWAFDDATPRVDVGGWLQGAVKEVGAGRAAFFGEAAMFTAQVTG
                     AEHRPMGMNAPGAEQNAQFVLNLLHWLTAAPEQASRRPE"
     gene            complement(1461..1799)
                     /locus_tag="PK399_02310"
     CDS             complement(1461..1799)
                     /locus_tag="PK399_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HPW11993.1"
                     /translation="MSELPPCPKCASTYTYEDGGQCVCPECGHEWMPGQADAVEDARV
                     VRDAVGNVLQDGDTVTVIKDLKLKGSSLVVKVGTKVRNIRLVDGDHDIDCKVDGIGQM
                     GLKSEFVKKA"
     gene            complement(1805..2242)
                     /locus_tag="PK399_02315"
     CDS             complement(1805..2242)
                     /locus_tag="PK399_02315"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HPW11994.1"
                     /translation="MPRLLVPLALFAAIGLAQAGEPARFGKPLPTSAPVAVADAVAAF
                     DQHAGKPLRFSGRITEVCQAEGCWMVLEDNGQSARVMFKDHAFVIPKDSSGRAQVGGV
                     LSRKELTAEQVAHLQEDSKGLAVSPVEYQILADGLELDAAPKS"
     gene            2357..3889
                     /locus_tag="PK399_02320"
     CDS             2357..3889
                     /locus_tag="PK399_02320"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW11995.1"
                     /translation="MSSNPPHVVPSGRKHWLPTSAGRLVRLLVVVGCIAIALLALRAW
                     QAAKATPLLPWHTLAAEEPSAPDILRGDWQAYVAHEAGLFARVRSELDAHMTPEDAHP
                     LNRYTAGSLSAPDRFQHDWNRSFVLEAKIPPRGVAVLVHGLTDAPYSMLRLATLYNAA
                     GYIAIVPRMPGHGTVPGALVRAERAQWQAAVAVAMDEARRRAGGRLPVHLVGYSNGAA
                     LALLHEVDRLRRGQPADANRMVLLSPMVRVSAFARYAGLAGLPALLPRYARSAWSEVL
                     PEYNPFKYNSFPVHAARESYLVTAALQEGLAVLAADGRLSGMPPILAFQSVVDDTVDA
                     SAVDRLLFDKLAADRNELVLFDVNRNRLLQPLQVAEKTAWADALLHGTAHAHAVTMVG
                     TTSLDDAAALARTRRANADALAMEALGIDYPTDVFSLSHIALPFAEDDALYGRIPRAG
                     HPLQLGAIALRGERNILVVPQASLERLSYNPFHDYMTARISSLLEAEQISTASRAATP
                     AH"
     gene            4144..4410
                     /locus_tag="PK399_02325"
     CDS             4144..4410
                     /locus_tag="PK399_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPW11996.1"
                     /translation="MPGWVLLDFGTDWCGHCIAARSAIDAWRDAHADIEHLRIEDGRG
                     RPLGRAYQVKLWPTLILLRDGREAARVVRPREARDLWPLEQALA"
     gene            complement(4839..5231)
                     /locus_tag="PK399_02330"
                     /pseudo
     CDS             complement(4839..5231)
                     /locus_tag="PK399_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(5304..5492)
                     /locus_tag="PK399_02335"
     CDS             complement(5304..5492)
                     /locus_tag="PK399_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HPW11997.1"
                     /translation="MLDSQEIECPYCGEVISLVLDPSAGAQRYIEDCQVCCRPITVVL
                     DVDDEGGAWVRVQSEHDA"
     gene            complement(5531..6079)
                     /locus_tag="PK399_02340"
     CDS             complement(5531..6079)
                     /locus_tag="PK399_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HPW11998.1"
                     /translation="MIRPLLIALVLACGAASAQDAALPNTSVQSLDLQRYAGQWHEIA
                     RLPMFFQRNCAANVTATYTPRADGKVGVRNACDDKDGESMVSEGVARPVAGQPGQLEV
                     RFAPDWLAWAPMTWADYWVVELDPDYQWAVVGGPSRKYLWVLSRTPTMPRAQFDAIRE
                     RAARRGYPVDRLLVSPNGMGDG"
     gene            6244..6648
                     /locus_tag="PK399_02345"
     CDS             6244..6648
                     /locus_tag="PK399_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW11999.1"
                     /translation="MSPRRETRAATKLLNATGNAAASVIAARMQAFSDPATLLSPWHQ
                     SEARRMTTEKLDAVTDGIQAAGMALAMLPARLLMIGARPASWTPGGWIAACNDVAELW
                     FGVGNAALRPARNTAVSNRRRLARRASNNTSA"
     gene            6766..7776
                     /locus_tag="PK399_02350"
     CDS             6766..7776
                     /locus_tag="PK399_02350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW12000.1"
                     /translation="MLARLQAELADTREQLRACQESHERNHSALCAIADGIAVVDTRG
                     RVTCLNPVASHLTGWKEDACLGRRLGKVVQFTDHQGRSIDVLSEGFSSDPDAIVSLVR
                     RDGHVILVDGAVAPVHAPDKRPLGAVVTFRNVTAATRLNRELAYHANHDALTGLHNRR
                     AFRAQLQRAIGHARDFGTSNALLYLDLDRFKAVNDRGGHRAGDELLRQLAVLLRKQLR
                     EHDTVARLGGDEFAVLLENATSADAGNVAEKIRSAVIDFTFAWDGAEFRIGTSIGLVE
                     FDNGDRSVERLLSIADGMCYAAKDGGRNRVMVHGAANRHAPAPGLDVSHRPGARRDDP
                     TP"
     gene            7876..8895
                     /gene="ltaE"
                     /locus_tag="PK399_02355"
     CDS             7876..8895
                     /gene="ltaE"
                     /locus_tag="PK399_02355"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HPW12001.1"
                     /translation="METIDLRSDTVTQPTAAMRAAMLDARVGDDVYGEDPTVNALQER
                     LADDLGFDAGLFVPTGTQSNLLALLAHCERGDEYIVGMEAHTYKYEGGGAAVLGSIQP
                     QPIVHAEDGTLPLDRIRAAIKPVDPHFARTRLLALENTWQGRPLPLDYLRDAHDLARS
                     QGLGIHLDGARLFNAAVACNVPAREIARHFDTVSICLSKGLGTPVGSVLLGPHALIDK
                     ARRWRKVCGGGWRQAGILAAAALHALDHHVARLQDDHDRAARLAEGLRSIPGIDLLGQ
                     HTNMVFIHVPGDRLRALDAHLRDAGIRISIGYLPTLRLVTHLDIDDDGIARTIAAFAA
                     FFAAG"
     gene            complement(8911..9945)
                     /locus_tag="PK399_02360"
     CDS             complement(8911..9945)
                     /locus_tag="PK399_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HPW12002.1"
                     /translation="MHRLLTALLPCMLAFSAAAAEPARPASYFDNTGREDAWQGGVRM
                     IPIHTPAGDFRVWTKRVGNNPRIKVLILHGGPGSTHEAYEAFDSFLPAGGVEYYYYDQ
                     LGSAYSDQPADMAPMLTIDRFVDEVEQVRTELGLDRENFYLYGHSWGGILAIEYALKH
                     QQHLKGLVVSNMMSSVPAYNRYANDVLMPEMDPAALAEIKQLEAAGKFDDPRYMELLM
                     PHYERHVLRRPAAEWPEPMLRGFGKLNKKVYVPMQGPSELGASGLLVDWDRTADLAKI
                     TVPTLVIGAKHDTMDPAWMRMMAGKFPRGDYLQLENGSHMAMYDDQQAYFAGLLQWLH
                     KVNAAAVATP"
     gene            10100..10981
                     /locus_tag="PK399_02365"
     CDS             10100..10981
                     /locus_tag="PK399_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW12003.1"
                     /translation="MTTTNSSVRWRGWLGPALLAAGALLTSGCLRVAFTVANQGQPPA
                     DASAAYASGPRNGIDIYRPTGATGTVPVMVFFYGGSWRNGAREDYRFVGRRLAQQGML
                     VLVADYRTYPETIFPGFVEDGASAVAWARANAAGWGGDPRCLFVAGHSAGAQIAALLG
                     TDQRYLAAQRMKPTDLAGVITLSGPLDFAITGDLVPVFGDPARWPDAQPVNFVDGDEP
                     PFLLIHGLRDKVVEAQDSRELAARLQRAKVPATLKLLPEGGHSTPLLGLYQPVQAPEV
                     LPAINAFVGACKRASEA"
     gene            11275..11484
                     /locus_tag="PK399_02370"
     CDS             11275..11484
                     /locus_tag="PK399_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPW12004.1"
                     /translation="MSDRQTGTVKWFNDAKGFGFITPQSGDDLFVHFRSIQGSGFKSL
                     QEGQKVSFKVAQGQKGLQAEEVQAE"
     gene            complement(11563..11955)
                     /locus_tag="PK399_02375"
     CDS             complement(11563..11955)
                     /locus_tag="PK399_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW12005.1"
                     /translation="MFNHIMVGSNDLDRSEAFYNAVLGVLGAGPPLRNRSPSGHMRLF
                     YRHDGSTFCVAEPIDGEPATCANGATIGFKCSSPEQAKEFHDVAVASGGTSIEDPPGL
                     RTSTLGSLYLAYVRDPDGNKLCALYRPR"
     gene            complement(11976..12401)
                     /locus_tag="PK399_02380"
     CDS             complement(11976..12401)
                     /locus_tag="PK399_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HPW12006.1"
                     /translation="MANPQPMLGPVIALVAWTAVMWAWMYATRIPAILRLRIRLDGNL
                     PRGEQMATLPPRVRWKADNYNHLFEQPILFYATALALALLGDASTASLALAWAYVALR
                     VVHSLWQALVNRIPVRFALFALSSLVLFALVARAAMHVF"
     gene            12541..13020
                     /locus_tag="PK399_02385"
     CDS             12541..13020
                     /locus_tag="PK399_02385"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW12007.1"
                     /translation="MRTLLRHAALLCLLAPVIAMASEPAEALLLQRANALVAAQLAFD
                     QPALEGLLARDYLEVSPVGDVDTRDEVIGFYSADAKAKAATGPMPIGATLDDVQVRVA
                     GDAATLVARETIRMPSPDGPRAIAMRVQFQFRLVDGQWCIATTQYTGIRPPARNKPD"
     gene            13091..13987
                     /locus_tag="PK399_02390"
     CDS             13091..13987
                     /locus_tag="PK399_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPW12008.1"
                     /translation="MKQHALLASLCLFTASCTGVANRDTPAPAPSTLTLASWNMEHLA
                     ERNGSGCRPRTDADYAAMRAYVDAMAADVIAFQEVESRAAAERVFDPSRYHVVIEARI
                     GSGRNGDCGGRPGLTINAQRTGFAIRKGLAFDRQPDVTDLQAGNPDLRSGVDVIVRPV
                     GGKPMRLLSVHLKAGCSAGDRNDACAALFQQVPAMERWIDARASEDLRFAVLGDFNRR
                     LVMPGDALWAEWDDGVPANADLARASGDRGARCNPRYKDFIDYIVLDRRAAAGQLDFD
                     EKTFAAEPLSDHCAVTTRLRLQ"
     gene            14094..14393
                     /locus_tag="PK399_02395"
     CDS             14094..14393
                     /locus_tag="PK399_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12009.1"
                     /translation="MDSISLLQETSTTPEALDAELDALESEMPNIQARAANMFVLAGA
                     WAERHDAIVAAAPPAALEAVEARLRRIGIRWGMMPGARMTAQFPALPAAGDTSTP"
     gene            complement(14468..14821)
                     /locus_tag="PK399_02400"
     CDS             complement(14468..14821)
                     /locus_tag="PK399_02400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPW12010.1"
                     /translation="MAFTTTASGLQFEDTVVGTGTEAKPGVNVTVHYTGWLYENGAQG
                     EKFDSSKDRGEPFIFPLGSGMVIKGWDEGVQGMKEGGQRTLIIPASLGYGTRGAGGVI
                     PPNATLKFDVELLGA"
     gene            14985..15827
                     /locus_tag="PK399_02405"
     CDS             14985..15827
                     /locus_tag="PK399_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HPW12011.1"
                     /translation="MFERFSRSWGLIKASAGVLAKDKELLVFPLLSAICTLIVAAAFL
                     LPAVGLGALDGLREDGMSVTAYVFAFLFYLVQYFVIFFFNSALVGAAMIRLEGGDPTV
                     RDGLRIAGSKVVPILGYAAIAATVGMVLRAIQERAGFLGRIVAGLIGVAWTLASFLVV
                     PVLVSRDIGPLDAVKESALLLKKTWGENLIGQGGVGLVFGLVFFALIIVGGAGIIGAA
                     TTGNGVLIGLMVALLIVALLVAALVQAALSGIYSAALYRYAVGAGDSEGFNAQLLGQA
                     FRTK"
     gene            complement(15885..16127)
                     /locus_tag="PK399_02410"
     CDS             complement(15885..16127)
                     /locus_tag="PK399_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12012.1"
                     /translation="MAPAKGEPIRAVAQVANGRAENHVARDVSAALQAVASNHYNVEV
                     DDGEDVLLKKQDGERDVVVTVVENTVQGMRIDIDAE"
     gene            16269..16886
                     /locus_tag="PK399_02415"
     CDS             16269..16886
                     /locus_tag="PK399_02415"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HPW12013.1"
                     /translation="MAPVSPSPLRTAVLAGAWLLAAAIALHFLASAHARYAQPDPAAY
                     GMFWTRRHWLWVHLAGGVLAMVLGSVQFIGGLRRRWPRVHRWIGRTYLLGVCIGLVGA
                     VALVATSPAPIAIRIAFTAIAAAWAGTASAGYRAIRGGDVSRHRRWMIRAYLVTLAPA
                     LFRLSLLVPGVMALASPLVMIPTLLWLSWTAPLAAYELARPRRGR"
     gene            complement(17002..17469)
                     /locus_tag="PK399_02420"
     CDS             complement(17002..17469)
                     /locus_tag="PK399_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12014.1"
                     /translation="MKTLALLLSVAALTACSDRQPPADAAAPADAAAVDATPAPAAAP
                     DEDTSPAPATPATPADSAKVEADYLGRWTGVEGMYLVVAAKPGGGVTLDMQWDLDNKG
                     MFDGSVTAEGLRFMRNGVAETALHTSGDATGLKYLAGKQDCLTVKPGEGYCRD"
     gene            17847..18362
                     /locus_tag="PK399_02425"
     CDS             17847..18362
                     /locus_tag="PK399_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPW12015.1"
                     /translation="MPEETMPATPANPSVGGAAMDAGKDIVTNASAAPNLSTLVAAVK
                     AADLVATLQGAGPFTVFAPTDDAFAKLPAGTVDGLLKPEMKADLAGLLTYHVVAGNVD
                     AAALTQQIEAGGGKATLTTVQGATLTAMSDGAGGITLTDGKGGTAKVTTADVRQSNGV
                     VHVIDSVLMPK"
     gene            18546..19115
                     /locus_tag="PK399_02430"
     CDS             18546..19115
                     /locus_tag="PK399_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HPW12016.1"
                     /translation="MGTPLRNKTIAILATDGFEQVELTEPKRAVEQAGATTRILSIKD
                     GQIQGMHHDKKGDTFAVDAQVADASIDDYDALLLPGGVANPDALRLDKAAVDFVRDFT
                     NSGKPIGVICHGPWVMVEADVVRGRKMTAWPSVRTDLRNAGAEVVDEEVVTDRGIVSS
                     RKPDDLPAFCRKIVEEFAEGNHAARRSAA"
     gene            complement(19252..19968)
                     /locus_tag="PK399_02435"
     CDS             complement(19252..19968)
                     /locus_tag="PK399_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12017.1"
                     /translation="MRFLLALLLCGLLPAAQAAPPKTPRAEVAVLATLHTLHAQVPAY
                     SLQVLRDSILRLAPDVLCIEVRPDRYAQRAPEANKVEYPDAVYPLIDAHRYRVYPMEP
                     AQPRFDAILKPYLQASREFAATNPAASAAFSAHAESMYAPLRAYWTSAARVNDAVTDA
                     QMRAKHALQEGLVGEGERAGWDAWNGEFLATIERAAAENPGKRIVVLVGAEHGYWLRE
                     KLAQRPGIRLLDTAALLADH"
     gene            complement(20197..20934)
                     /locus_tag="PK399_02440"
     CDS             complement(20197..20934)
                     /locus_tag="PK399_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW12018.1"
                     /translation="MKRLTTCLLTALCATPAFAQVPAAPKVPTLIALGFKADIGVDGV
                     PANVTPDASLPPALQAMVRKRVSEWRYRPATWQGRTLARPVSQQITAEVLQVAGGGFA
                     MRIKEVGGAPPPMTADGKPGGTMMVPPHYPLESRKRGVEGTLVYAVRVDRKGAAGEIT
                     LLSPEKLERRMRPLDEAARTAIQQWPFSVIEVDGVAIECQAVIPMQFALDRPKPDMPD
                     LAPYRARLGDACPASPALLTPVAETLL"
     gene            complement(21131..21358)
                     /locus_tag="PK399_02445"
     CDS             complement(21131..21358)
                     /locus_tag="PK399_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12019.1"
                     /translation="MDVLDEISHLLSDYGLPEGDVKRLVRGLEFDLDQMAVRDALGGN
                     LSVAQKQFIRASILSGTRLIDIVSALKSSVT"
     gene            complement(21509..21853)
                     /locus_tag="PK399_02450"
     CDS             complement(21509..21853)
                     /locus_tag="PK399_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12020.1"
                     /translation="MPVSAAEPGLAGDWVVDLATDPAVPYTQPMQLALRPDGGVEGSF
                     YNSRIEAGRWKSDRGRVCASFRTSDGVGPYHTSVCLDGEIARGQTWAEHRSFLFNWNA
                     VRAPVAGGRDAE"
     gene            complement(22083..22763)
                     /locus_tag="PK399_02455"
     CDS             complement(22083..22763)
                     /locus_tag="PK399_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12021.1"
                     /translation="MNRVAIWFFISLLFVASGAHAQFASKDRARHLTEQLQSSSMAAR
                     MEASRQIFGSGINDKGLNDLLASIIGSALPSLDKDSPRVEELAWHVKALSASGDETYM
                     PVILLAAQSPVKKLARHAKDAKAILEKQSAEGRPYLIPTKVAVITETQAENCAYVDQQ
                     MCKTGRGAEKCIEWHQTGAASRGANAVMLLNSNTKVGPATVIPIGSSAYVSQASKTTM
                     MASYYLCQ"
     gene            complement(22854..23576)
                     /locus_tag="PK399_02460"
     CDS             complement(22854..23576)
                     /locus_tag="PK399_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW12022.1"
                     /translation="MGLAAVLGAGFPARAASPPRALSLDFQADVQVDGRPVNIAPDPG
                     LSPALQALVRKQVAGWRYIPGTWQGKPVPGHVSQRIVVDVVPAASDGFSLRIKSVTSV
                     PMVLDSKGAPSSRAMNPPSYPIEAQRQGVEETLVYAIRRDPQGKTSDVELLDSQAGNN
                     WKKTFDAAARRAIGQWQLEPVEVDGQRIDCRLITPITFRLGVGRSLPPATMPDMRPYL
                     PRVPDGCPLPPVLDTKVEGVSL"
     gene            complement(23596..24174)
                     /locus_tag="PK399_02465"
     CDS             complement(23596..24174)
                     /locus_tag="PK399_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12023.1"
                     /translation="MSKIPDRAYAQMREAKAASERLVSLVAAKRAGWTQAQTDELSCI
                     YQILKGTHGALNSILETNASLPVKIPQAAFPHMREAKAASDRLQSLVGTKRAGWTQAQ
                     TDELSCIYQVLRGTHAALNSMLETNASMSGKIPQAAYSRMQEAKAASVRLAALVGTRR
                     AGWTPAQADQLSCIYQVLKGTHGALNSLIESN"
     gene            complement(24651..25460)
                     /locus_tag="PK399_02470"
     CDS             complement(24651..25460)
                     /locus_tag="PK399_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12024.1"
                     /translation="MSSEGESGSHVQLARKTSAAKRLRTYFEHLNSFSNNKPLHQQWA
                     RVFGVSSIDASQPQLVATEVARKLSLVGRQVDLVERRLRNEHPTLTPNTTSNQAHRLR
                     LILANCLSSLQAPAGNLRNELTQDLLKTLEFWDELLPCDEVDLADSRLNEVADLLDQA
                     EQLARNSSNADLAEALVSIIQLLRRAINDGAVGGEDEFHRNVRAAYTEAIKNEALIKQ
                     NSDDVAVSALKKVWDRFSEVCAPVILADSFLTAASRLKEIGGMAMKLLGGG"
     gene            complement(25467..26264)
                     /locus_tag="PK399_02475"
     CDS             complement(25467..26264)
                     /locus_tag="PK399_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HPW12025.1"
                     /translation="MAVRIEKKIKGYAVVTPEDKAKDAVKADSVTRAQVEAELPVADV
                     IQMHERIERPEVLIGSTYKIKSPLVEHAMYVTINDIVLNAGTDHEHRRPFEVFINSKS
                     MEHFQWIVALTRIMSAVFRKGGDVTFLVEEMKAVFDPKGGYFKAGGVYMPSLVAELGM
                     IMEDHLKSIGMMHDPEMSEHTRALIAEKRRAFEGRSKKNSDVVSAGEMGGAMGSSTAD
                     HAEDIEVTGDGASFPPSATMCHKCSTKALVLMDGCATCLNCGYSKCG"
     assembly_gap    26449..26548
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26549..27107)
                     /locus_tag="PK399_02480"
     CDS             complement(<26549..27107)
                     /locus_tag="PK399_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12026.1"
                     /translation="MSNGNGVAATLSDAAESVKEKASEIGDAVASTASATVAKAKKAA
                     KSAEKSVKSKVANAKKAVAKRTEKASKAVKKTVANAKKKLAAASADAKKEVASLKKKV
                     AGKKTAAKKATKKVAKKATAKKAAVKKAVKKAPAKKAAAKKAPAKKAAAKKAPARKAA
                     AKKAPARKAAAKKAPMKKAAAKKAPA"
     gene            complement(27265..>29120)
                     /locus_tag="PK399_02485"
     CDS             complement(27265..>29120)
                     /locus_tag="PK399_02485"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPW12027.1"
                     /translation="TSTINCTVSGTITDSMDGILEKVHEAGLTLKAGCGIGYEFSTLR
                     PRGAFVAGAGAYTSGPMSFMDIYDKMCFTVSSAGGRRGAQMGTFEIAHPDVKDFIRAK
                     REDGRLRQFNLSLLITDGFMDAVKNDSDWPLVFPVAEKEADEIDLANAEQVVWREWPT
                     HAGYISREDGLVACKIYGQIRARHLWDMIMVSTYDYAEPGFILIDRVNEMNNNWWCET
                     IRATNPCGEQPLPPYGACLLGSINLTKFVRHPFTDKASFDWEEYKEVVRVFTRMLDNV
                     VEVNGLPLQQQRDEIARKRRHGMGFLGLGSTVTMLQMKYGSAASCEFTERIARDMAVA
                     GWEMGLSLAKEKGAAPIMDEVFEVTAEMLRKRPEMAKDGWKVGQEISGKVLHAKYSRY
                     MQRVAQVAPDLVAELAEVGARFTHHSSIAPTGTISLSLANNASNGIEPSFAHHYSRNV
                     IREGKKTKEKVDVWSFELLAYRELVNPKAMPYAEDAASKLPDYFISADDITPKAHVDV
                     QAAAQKWVDSSISKTANVPTDYPYEDFKDIYRYAHEQGLKGCTTFRFNPAAFQGVLVK
                     DADLENTTYRFELEDGSVVEVKGNEQIEYDGELHTAANLFDALKEGYYGKF"
BASE COUNT         4584 a         9931 c         9459 g         5046 t
ORIGIN      
        1 caccagcacg tcgccattcg gcagcacatg cagccagcgc ggatgctcca ggccgtcggc
       61 gaaacggctg acgaccaggc cgcgcgcagg cgtgggtgtc gcgccctgcg gccatccggt
      121 ggccggcgcg atgtccaccg tggggatcaa tgcacgctgc ggaggcggca acgcaggatc
      181 cacgccctcg gtgctgcccg gcgacaacgc ctggcccccg ccgcaacccg cgagcagtcc
      241 ggtgcacagg agcagcggca aggtggggcg catgcagggt tccttgatcg acgggagctg
      301 ggaaggcact ggccggctgc gccttcacca gaggggagag gcgcgtgatc gctggcgggc
      361 caatgcgagg caacggtttt acccccgccg cggtgaatgc cgtgtgggcg caaccgaatg
      421 ccgatgggcc accggtgcgg cgcgcccgcg atcactccgg acgacgcgat gcctgttccg
      481 gcgcagcggt gagccagtgc agcaggttca ggacgaactg cgcgttctgc tcggcgcccg
      541 gtgcgttcat ccccatcggg cgatgttcgg caccggtgac ctgtgcggtg aacatcgccg
      601 cctcgccgaa gaaggccgcg cggcccgcgc cgacctcctt caccgcgccc tgcagccagc
      661 cgccgacatc gacgcgcggc gtggcgtcat cgaaggccca cgctttcgca ggatgcaagg
      721 acacgaagcc ggccggcaac accatcagcg gttgtgcgcc cggtgcacgg aacgcctgcc
      781 cggtgaacga ccgcagttgc gtgatcgctt cgccggtgtg gcgtccgcgc aggatcgggt
      841 ggtcggccag cgtgccctcg tcgagccgga acagcgtggg tttcgccttc gcttcgtcgt
      901 tgacgcctgc gaacgcgaag ccatccgtga attccacgtc gaaggccgcg gccaggttcg
      961 atgcggcacc cgccagcggc atgtggtcgg cgatcaacag caggcggcca ccgccctgca
     1021 cccagtcgcg caccgcggcg atctcagccg gcgcaaaggc ggagggcgtg ggcgtcggat
     1081 acgtattcca gtcggcgtcg ctcggttgcg cgttggcgat gaccagcgcc gcgcacgcgg
     1141 acaaactcgt ttcatcgaag gcggcaccgt tcggcgcgac cacgtagccg tcgcggcgca
     1201 gcagcgcgcc gaacggacgg aaacgcccgt ccagggtgtg gaaattgtgg tgcgcctcgt
     1261 ccaggcagac caccgggccc ttgccttcgg cgaacgccgg cggcgatacg gccggcgcga
     1321 aatcgcgatc tgccacctgt tgcgccacgc acggcaggct ggcggacaac agcagaacga
     1381 ggcaggcgat tcgtttcatg ccccgagcgt cacgcgccac ggcgcgccgc gcatgtgccg
     1441 gaaggcgcgg atgggtggcc tcaggccttc ttcacgaatt cggatttcag gcccatctgg
     1501 ccgatgccgt cgaccttgca gtcgatgtcg tggtcgccat cgaccaaacg gatgttgcgc
     1561 accttggtcc ccaccttcac caccagcgac gagcccttga gcttgaggtc cttgatcacg
     1621 gtgaccgtgt cgccgtcctg caggacattg ccgaccgcgt cgcgcaccac cctggcgtct
     1681 tcgacggcat cggcctggcc cggcatccac tcatgcccgc attcggggca gacgcactgg
     1741 ccgccgtcct cgtaggtgta ggtcgacgcg cacttcgggc agggcggcag ttctgacatg
     1801 gcgttcacga cttcggcgcc gcgtcgagct ccaggccgtc ggccaggatc tggtactcga
     1861 ccggcgacac cgccaggccc ttgctgtctt cctgcaggtg cgccacctgc tccgccgtga
     1921 gttccttgcg cgacagcacg ccgccgacct gcgcccgccc gctgctgtcc ttcgggatca
     1981 cgaaggcgtg gtccttgaac atcacccgcg cagactggcc attgtcctcc agcaccatcc
     2041 agcagccttc ggcctggcag acctcggtga tgcggccact gaagcgcagc ggcttgccgg
     2101 catgctggtc gaacgccgcg acggcatcgg ccacggcgac cggggcggag gtgggcaggg
     2161 gcttgccgaa gcgcgcgggt tcaccggctt gggccaggcc gatggcggcg aacagtgcga
     2221 gcgggacgag gaggcgcggc atggggatgg cccgatggga ttcgacatcc aaggataacg
     2281 cgtggcgcct gccggcccga agccggcgat ttgcacgcat catgcggggc atgcctgcat
     2341 cccggatccc ctgcgcatga gctcgaaccc accgcacgtc gtgccatccg gacggaagca
     2401 ttggctgccg accagtgcag gccgcttggt gcgattgctg gtggtggtgg ggtgcatcgc
     2461 catcgcgctg ctggccctgc gcgcatggca ggcggccaag gccacgccgt tgctgccctg
     2521 gcacaccctg gcggccgaag aaccctccgc accggacatc ctgcgtggcg actggcaggc
     2581 gtacgtcgcc catgaagccg gactgttcgc acgcgtgcgc agcgagctcg atgcccacat
     2641 gacgccggaa gacgcgcatc cgctcaaccg ctacaccgcc ggcagcctga gcgcgcccga
     2701 ccgattccag cacgactgga accgctcgtt cgtgctcgag gcgaagatcc caccgcgtgg
     2761 cgtcgcggta ctggtgcacg gcctgaccga cgcgccctac agcatgttgc ggctggccac
     2821 gctgtacaac gccgcgggct acatcgccat cgtgccgcgc atgcccgggc atggcacggt
     2881 gccgggcgcg ctggtgcgcg cagagcgcgc gcagtggcag gccgcggtgg cggtggcgat
     2941 ggacgaagcg cggcgccgcg ccggtgggcg cctgccggtg cacctggtcg gctactcgaa
     3001 tggtgcagcg cttgcgctgc tgcatgaagt cgatcggttg cgacgcggcc agcctgccga
     3061 tgcgaaccgc atggtcctgc tgtcgccgat ggtccgggtc agtgccttcg cccgctatgc
     3121 gggactcgcg ggattgcccg cgttgctgcc gcgctacgcg cgctcggcgt ggtcggaggt
     3181 gctgcccgag tacaacccgt tcaagtacaa ctcgttcccg gtccacgccg cccgcgagtc
     3241 ctacctggtc actgccgccc tgcaggaagg cctggcggtg ctggccgccg atggccgcct
     3301 gtccggcatg ccgccgatcc tcgccttcca gtcggtagtc gacgacaccg tcgatgcatc
     3361 ggcggtcgac cgcctgctgt tcgacaaact cgccgcggac cgcaacgaac tggtgttgtt
     3421 cgacgtcaac cgcaatcgcc tgctgcaacc gctgcaggta gccgagaaaa cggcatgggc
     3481 cgacgccttg ctgcacggca ccgcgcatgc gcacgcggtg acgatggtgg gtacgacctc
     3541 actggacgat gccgccgcgc tggcgcgcac gcgccgcgcg aatgccgatg cgctcgcaat
     3601 ggaggcgctc ggcatcgact accccaccga cgtgttctcg ctctcgcaca tcgccctgcc
     3661 attcgccgag gacgatgcgc tgtacgggcg catcccgcgc gctggccatc cgctgcagct
     3721 cggcgccatc gcgctgcgtg gcgaacgcaa tatcctggtg gtgccgcagg ccagcctgga
     3781 acggctcagc tacaacccgt tccacgacta catgacggca cggatttcga gcttgctgga
     3841 ggcggaacag atatccacgg catcgcgcgc agccacgcca gcgcactgat cacccgcttc
     3901 gacgcgtcgc caacatgaag ccgccatgac tcgcggcagg cggatgcggt gcaattcacc
     3961 ctcgccttcc tcgcacatcg cgacgatgac ggcatcccac acggatgcat ccacgatgtc
     4021 gaacagcatc ttcctgccgt tgcgtgcggg aacctcacct gcaacgccac atcgggcgtc
     4081 gccgtgacgt ctcgccacct tccgtatcgc gacctgagcc gcgcgccggc cacggtcgcc
     4141 gccatgccgg gctgggtgtt gctcgacttc ggcactgact ggtgcgggca ttgcatcgcc
     4201 gcgcgctccg ccattgacgc atggcgcgat gcgcatgcgg acatcgagca cctgcgcatc
     4261 gaggacggcc gcgggcgtcc gctcggacgc gcgtaccagg tcaagctgtg gccgaccttg
     4321 atcctgctgc gcgacggccg agaagcggcc cgcgtggtgc ggccacgcga ggcgcgcgac
     4381 ctgtggccgc tggagcaggc gctggcgtga acgacgcgtt cgtcagtgct tgtccttcgc
     4441 gtgttgctgg cgtacccacg ccatgatgtc gacgaagggc gcggtccgta gtcgtcgcgg
     4501 tgcgcatcga gataggccag caattcctcg tgcgcctgcg ccgatacctg caggtggtcg
     4561 ccgccgatgc cgtggaaggt gaagttgacc atcgtgccct tgcggcgggc ctcctccacc
     4621 agcgcgatca gttgtgcgcc actcacaccc acaggagcgg ccaccggcac cgcggatgga
     4681 tccagcgtcg ccatgtcgtc gaccaccgca ttgccgatca gcttgatgcc ggcgaacagc
     4741 ggcgccaccg catcgaggta gttgccatcc atcgccgccg cctcgccgca gggcacggtg
     4801 taggtgcgca cgctgcggcc atcgatggcc tgcaacatgg tgctggccag ttcgacctgg
     4861 tcgcgcatct gcgccaccgt ggtggtgtcc aggttgcgtt ggggggcgac ccagctacgg
     4921 tcgggaccct tgccggagca ctggtggaac aggctgtggt tgccgagttc gtgcccattg
     4981 gccgcggccg tgcgccagtc cgccaggcgc gtgcgcaccg attgcgccga caggatcagg
     5041 tagaaactgc ccttcagccc atgccgatcc agcgccggga tggcggtgtc cagttgcgaa
     5101 tccagcgcat cgtcgtaggc caggctgacc gcggccttgc gcccttccgg ccaggcgaag
     5161 ccgtcgcctg cagacgcact ggaagaccat gcgatggcgt acagcaggaa cacaggcagg
     5221 caggcgcgca tgggcggctc ggatgcggac gatgcggcag cataccgacc tgcctcgacc
     5281 acgaatcgat ccctcgcgac gcgtcaggcg tcgtgctccg attgcacgcg cacccacgca
     5341 ccgccttcgt catccacatc gagcaccacc gtgatcggac ggcagcagac ctggcagtcc
     5401 tcgatgtagc gctgcgcccc ggcactggga tccagcacca ggctgatcac ctcaccgcaa
     5461 tacgggcatt cgatctcctg gctatcgagc atgcgcagct ccggattcgg atcgcatcat
     5521 catgcaacga tcagccatcg cccatgccgt tgggcgacac cagcaggcgg tcgaccgggt
     5581 agccgcgtcg tgccgcgcgc tcgcggatcg catcgaattg cgcgcgcggc atcgtcggcg
     5641 tgcgcgacag cacccacaga tacttgcggc tcggcccgcc caccacggcc cactggtagt
     5701 cgggatccag ctccaccacc caatagtcgg cccaggtcat cggtgcccat gcgagccagt
     5761 ccggcgcgaa ccgcacttcg agctgccccg gctgccccgc gaccgggcgg gcaacgcctt
     5821 cggacaccat cgactcgcca tccttgtcgt cgcaggcatt gcgcacgccg accttgccgt
     5881 ctgcgcgcgg ggtgtacgtc gcggtgacgt tggccgcgca gttgcgctgg aagaacatcg
     5941 gcaggcgggc gatttcatgc cattgcccgg catagcgctg caggtccagc gactgcacgg
     6001 aggtgttcgg caacgcggcg tcctgcgcgg acgcggcgcc acaggccagc acaagggcga
     6061 tcagcagggg tcggatcatg cgacgacgct ccatcagggg acgcgcagcc ttgcggatgg
     6121 cgcgtgcgtg cagggtgaag ccagccgcgt gcatccgccg gaccggcatc gccggttgct
     6181 gcacgggctt ttcatccgcc gtcctgcatg gtgcccgccg cacccattcc ccggacacca
     6241 cgcatgtctc ctcgccgcga aacccgcgcc gccaccaagc tcctcaatgc gacaggcaac
     6301 gcggcggcca gcgtgatcgc tgcgcgcatg caggcctttt ccgatccggc gacgctgctg
     6361 tcaccgtggc accagtcgga agcacggcgc atgaccacgg agaagctcga tgccgtcacc
     6421 gacggcatcc aggcggccgg catggccttg gcgatgctgc cggcacggct gttgatgatc
     6481 ggtgcgcgcc ccgcgtcatg gacaccgggc ggctggatcg ccgcgtgcaa cgacgtcgcc
     6541 gagttgtggt tcggcgtggg caacgcggcc ctgcgccccg ccaggaacac ggcggtgagc
     6601 aaccggcgcc ggctggcgcg tcgcgcgtcg aacaacacct ccgcgtgacc tgattcgccg
     6661 gcttcaccgg aatttggcac gtcgcaggtc atcttcccgt cgatgaccag ccccgggcca
     6721 acggctccgc catcctccag tgggggcaac ggcgagacaa cgcaaatgct ggcgcgcctg
     6781 caagccgagc tggccgacac gcgcgagcag ttgcgcgctt gccaggaatc gcacgagcgg
     6841 aaccacagcg ccctgtgcgc catcgcggac ggcatcgccg tcgtcgatac gcggggccgg
     6901 gtgacctgcc tcaacccggt ggcatcgcac ctgacgggct ggaaggagga tgcgtgcctg
     6961 ggccggcggt tgggcaaggt ggtgcagttc accgaccacc agggccgcag catcgatgtc
     7021 ctgtccgaag gcttttccag cgacccggat gcgatcgtct cgctggttcg ccgcgatggc
     7081 cacgtgatcc tggtcgatgg cgcggtcgcg cccgtgcacg cgcccgacaa gcggccgctt
     7141 ggcgcggtgg tcacgttccg caatgtcact gccgctaccc ggttgaaccg cgaactggcg
     7201 tatcacgcca accacgacgc gctgaccggc ctgcacaatc gccgcgcctt ccgcgcgcag
     7261 ctgcaacgcg ccatcgggca cgcccgcgac ttcggcacca gcaacgcgct gctttatctc
     7321 gacctggacc gattcaaggc ggtcaacgac cgcggcggtc accgcgccgg cgacgaattg
     7381 ctgcgccaac tggcggtgtt gctgcgcaag cagctgcgcg agcacgacac cgtcgcgcgc
     7441 ctgggcggcg acgagttcgc ggtgctgctg gagaacgcca catccgccga tgccggcaac
     7501 gtggcggaaa agatccgcag cgcggtcatc gatttcactt tcgcctggga tggcgccgag
     7561 ttccgtatcg gcaccagtat cggcctggtc gaattcgaca acggcgaccg ctcggtcgag
     7621 cgactcctgt cgatcgccga cggcatgtgc tacgcggcca aggatggcgg acgcaatcgc
     7681 gtgatggtgc acggcgcggc gaaccggcac gcaccggcac cgggcctgga cgtttcccac
     7741 cgtcccggcg cgcgccggga cgaccccacg ccgtagcgca cgccagcgcc gcccaccggg
     7801 cgcaggatgc aggacttggc gcagcgaccc cggcacatcc gggcacaatg cgctggacga
     7861 ccgaaggaac aaggcatgga gaccatcgac ctgcgcagcg acaccgtgac ccagcccaca
     7921 gccgcgatgc gcgcggcgat gctggacgcc cgcgttggcg acgatgtcta cggcgaggac
     7981 cccacggtca atgcgctgca ggaacggctg gccgatgacc tcggcttcga tgccgggctg
     8041 ttcgtcccca ccggcaccca atccaacctg ctggcgctgc tcgcgcattg cgagcgcggc
     8101 gacgaataca tcgtcggcat ggaagcgcat acctacaagt acgaaggcgg cggggcagcg
     8161 gtgctgggct cgatccagcc gcagccgatc gtccacgccg aggatggcac gctgccgctg
     8221 gatcgcatcc gcgcggcgat caagccggtg gatccgcatt tcgcgcgcac ccgcttgctg
     8281 gcgctggaaa acacctggca gggccgtccg ctgccgctgg actacctgcg cgacgcgcac
     8341 gacctggcgc ggtcgcaggg actcggcatc cacctcgatg gggcgcgcct gttcaacgcc
     8401 gcggtggcct gcaacgtgcc ggcgcgcgag atcgcgcggc acttcgacac ggtctcgatc
     8461 tgcctgtcga agggattggg cacgccggtg ggctcggtac tgctgggccc gcatgcgctg
     8521 atcgacaagg cgcggcgctg gcgtaaggtc tgcggcggcg gctggcgcca ggccgggatc
     8581 ctggcggccg ccgccctgca tgcgctcgac catcatgtcg ccaggctgca ggacgaccac
     8641 gaccgcgcgg cgcggctggc cgaaggcctg cgttcgatcc ccggcatcga cctgctgggc
     8701 cagcacacca acatggtgtt catccacgtc cccggcgatc gcctgcgtgc gcttgatgcc
     8761 cacctgcgcg atgccggcat tcgcatcagc atcggctacc tgccgacgct gcgcttggtc
     8821 acgcacctgg atatcgacga cgacggcatc gcgcgcacca tcgccgcgtt cgcggcgttc
     8881 ttcgcggccg gctgaaggcc cgcgcccgga tcagggcgtc gccaccgcgg cggcgttcac
     8941 cttgtgcaac cactgcagca ggccggcgaa ataggcctgc tggtcgtcgt acatcgccat
     9001 gtggctgccg ttctccagtt gcaggtaatc gccgcgcggg aacttgccgg ccatcatccg
     9061 catccatgcc ggatccatgg tgtcgtgctt cgcgccgatc accagcgtcg gcacggtgat
     9121 cttcgccagg tcggcggtgc gatcccaatc caccagcagg ccgctggcgc cgagttcgct
     9181 cggcccctgc atcggcacgt agaccttctt gttcaacttg ccgaatccac gcagcatcgg
     9241 ctccggccac tccgccgccg gccggcgcag cacgtggcgc tcgtaatgcg gcatcagcag
     9301 ttccatgtag cgcgggtcgt cgaacttgcc ggccgcctcc aattgcttga tctccgccag
     9361 cgccgctgga tccatttccg gcatcagcac gtcattggcg taacggttgt acgcgggcac
     9421 gctggacatc atgttcgaga ccaccaggcc cttgaggtgc tgctggtgct tcagcgcgta
     9481 ttcgatggcg aggatgccgc cccacgaatg cccgtacagg tagaaattct cgcgatccag
     9541 gcccagttcc gtgcgcacct gctccacttc atcgacgaag cgatcgatgg tcagcatcgg
     9601 cgccatgtcg gcgggctggt cgctgtaggc ggagcccaac tggtcgtagt agtagtactc
     9661 cacgccgccc gccggcagga aactgtcgaa ggcctcgtat gcctcgtggg tggagccggg
     9721 gccgccatgc aggatcagca ccttgatccg cgggttgttg ccgacgcgct tggtccacac
     9781 ccggaagtcg ccggccggcg tgtggatcgg aatcatccgc acgccgccct gccaggcatc
     9841 ctcgcgaccg gtattgtcga aatacgacgc gggcctggcg ggttcggccg cggcggcgga
     9901 gaaggcgagc atgcagggca acaacgcggt cagcaggcga tgcatggcga ctccgggatg
     9961 gcgatggctc gcgcgaatgt acgccgatgg ttcgcccggg tcaccggcgg cggcgtccgg
    10021 gttccgccac ctcgtggcgc atgcgcatgg gccggggtcg cctgcatccg cttcacgcgg
    10081 cgtcgcgtat ccaaggcgca tgacgacgac aaactcctcc gtgcgctggc gtggctggct
    10141 cggccccgcc ctgctcgccg ccggtgcatt gctcaccagt ggctgcctgc gcgtcgcctt
    10201 caccgtggcc aaccaagggc aaccgcccgc ggatgccagt gcggcctatg cgagcgggcc
    10261 gcgcaacggc atcgacatct accggcctac gggggcgacc ggcaccgtcc cggtgatggt
    10321 gttcttctac ggcggaagct ggcgcaatgg cgcgcgcgag gactaccgct tcgtgggtcg
    10381 tcggcttgcc cagcagggca tgctggtgct ggtggccgac taccgtacct atcccgagac
    10441 gatcttcccc ggcttcgtcg aagacggcgc cagcgcggtt gcgtgggcgc gcgcgaatgc
    10501 cgcgggctgg ggcggcgatc cgcgctgcct gttcgtggcc ggccattccg cgggcgcgca
    10561 gatcgcggcg ctgctgggca ccgaccagcg ctacctggcc gcacagcgga tgaaaccgac
    10621 cgacctggcc ggcgtcatca ccttgtcggg accgctggat ttcgcgatca ccggcgacct
    10681 ggtgccggtt ttcggcgatc ccgcacgctg gccggacgcg cagcccgtca atttcgtcga
    10741 tggcgacgaa ccgcccttcc tgctcatcca cggcctgcgc gacaaggtcg tcgaggcgca
    10801 ggacagccgc gaactggcgg cccggctgca acgcgcgaag gtgccagcca ccctgaaact
    10861 gctgccggaa ggcgggcaca gcacgccgct gctgggcctg taccagcccg tgcaggcacc
    10921 ggaagtgctg ccggcgatca atgccttcgt cggtgcgtgc aagcgggcca gcgaggcctg
    10981 aaaccgccgc agggcgtgtg aaatcgccgt tccggtgcgc cacgcgcgtc gcaaatcgca
    11041 gcccgctccg ccactccgtt cacaaaagag ggcgaaagtg aggtacggtg cgtgcactgt
    11101 tcaaagcggg atcacggtac atcgtggctc cgatgcgtgt tgatcaacag gttccagggc
    11161 cttcaacggc ctctccagac gatctgctcc atcgcttcgc gttacccctt gctcgacagt
    11221 ccggcaccgc atcccgcggg ccgtgcctta tccactgttc caaggagtaa caccatgtct
    11281 gatcgtcaaa ccggtaccgt caagtggttc aacgacgcca agggcttcgg cttcatcacc
    11341 ccgcagagcg gcgatgacct gttcgtccac ttccgttcca tccagggctc gggcttcaag
    11401 tccctgcagg aaggccagaa ggtctcgttc aaggttgcgc agggccagaa gggcctgcag
    11461 gccgaagaag tccaggccga gtaagttcta ctcgacgctt gcgccgcgaa agcggcacca
    11521 ggaaccccgg gcggcaacgt ccggggtttt tgtttgcgcc gtttaccttg gccgatacag
    11581 cgcgcacagc ttgttgccgt cggggtcgcg cacataggcc aggtacaggc tgcccagggt
    11641 gctggtgcgc aagccgggtg gatcctcgat cgaggtgcca ccggacgcga ccgcgacatc
    11701 gtggaattcc ttcgcctgct ccggcgaact gcacttgaag ccgatcgtcg cgccgttcgc
    11761 gcaggtcgcc ggctcgccat cgatcggctc ggccacgcag aaggtgctgc cgtcgtggcg
    11821 gtagaacagc cgcatgtgcc cgctgggcga ccggttgcgc agcggcggac cggcgcccag
    11881 cacgccgagt acggcgttgt agaacgcctc ggaacggtcg aggtcgttac tgccgaccat
    11941 gatgtggttg aacatgcggc tggatcctcg tggtgtcaga agacgtgcat cgccgcgcgc
    12001 gcgaccagcg cgaacagcac cagcgacgac agcgcgaaca gcgcgaagcg caccgggatg
    12061 cggttgacca gcgcctgcca caggctgtgc accacccgca gcgcgacgta ggcccaggcc
    12121 agcgcaagcg acgcggtgga cgcatcgccc agcagggcca gggccaacgc ggtcgcgtag
    12181 aacaggatcg gctgctcgaa caggtggttg tagttgtcgg ccttccagcg cacccgcggc
    12241 ggcagcgtgg ccatctgttc gccgcgcggc aggttgccgt ccaggcggat gcgcagccgc
    12301 aggatggcgg ggatgcgggt cgcatacatc cacgcccaca tcaccgcggt ccacgcgacc
    12361 agcgcgatga ccgggccgag catcggttgc gggttggcca tcgtggcact ccagggcagc
    12421 aaggcatcgg cgacgcggag caaccgcgtg cgcgaggcgg ccaatgtata tgaccttgcg
    12481 cactggagca ggcccgtgcc ggcacgccag gatgcagcca ccccacgctg gaaccccgcg
    12541 atgcgcaccc tgctccgcca cgccgccctg ctgtgcctgc tcgctcccgt catcgccatg
    12601 gccagcgaac ccgccgaggc cctgctcctg cagcgcgcca atgcgctggt ggccgcgcaa
    12661 ctcgcgttcg accagcctgc gctggaaggc ctgctggcca gggactacct ggaagtgtcg
    12721 ccggtcggcg acgtcgatac gcgggacgag gtcatcggct tctattcggc ggacgccaag
    12781 gccaaggcgg caacgggccc gatgccgatc ggcgccacgc tggacgacgt gcaggtccgg
    12841 gtggcgggcg atgccgcgac gctggtcgca cgcgaaacca tccgcatgcc atcgcccgat
    12901 gggccgcgcg cgatcgccat gcgcgtgcag ttccagttcc gcctggtcga tggccaatgg
    12961 tgcatcgcga cgacgcagta caccgggatc cggccaccgg cgcgcaacaa gccggactga
    13021 ggttacgctc cgccaccccg tccgcgcgac caacccaggt cgtgcgcgcc acgccatcgg
    13081 atctccccgc atgaaacaac acgccctgct cgcctcgctc tgcctgttca ctgcgtcctg
    13141 cacgggggtc gccaaccgcg acacgccggc acccgcgcct tcgacgctga ccttggccag
    13201 ctggaacatg gaacacctgg ccgagcgcaa tggctcgggg tgccggccgc gcaccgacgc
    13261 ggactacgcg gcgatgcgcg cctacgtgga cgccatggcc gccgacgtga tcgccttcca
    13321 ggaagtggaa tcgcgcgccg cggccgaacg cgtattcgat ccgtcgcgct accacgtggt
    13381 gatcgaggcg cggatcggca gcggccgcaa cggcgattgc ggtggccgcc ccggcctgac
    13441 catcaatgcg cagcgcaccg gcttcgcgat ccgcaaggga ctggccttcg atcgccagcc
    13501 cgatgtgacc gacctgcagg ccggcaatcc ggacctgcgc tccggggtgg acgtgatcgt
    13561 ccgccccgtc ggcggcaagc cgatgcgcct gctgtccgtg cacctcaagg cgggttgcag
    13621 cgccggggac cgcaacgacg catgcgcggc gctgttccag caggtgcccg cgatggagcg
    13681 atggatcgac gcgcgcgcct cggaagacct gcgcttcgca gtgctaggcg acttcaaccg
    13741 ccgtttggtc atgccgggcg atgcgctctg ggcggaatgg gacgatgggg tgcctgccaa
    13801 cgccgatctc gcgcgtgcct ccggcgaccg cggcgcgcgc tgcaatcccc gctacaagga
    13861 tttcatcgac tacatcgtgc tggatcgccg cgccgcggcc ggccagctcg acttcgacga
    13921 aaagaccttc gccgccgaac ccctgagcga ccactgcgcg gtgaccacgc gcctgcgctt
    13981 gcaataggcc aatgccggcg atgcgctgat tcacggcgcg tggacaccgc gctcacgacg
    14041 gctctacccg gcatgtcgga tcgtcgccat cgacgacccg agaacatgcg cacatggaca
    14101 gcatcagcct gttgcaggaa acctccacca cccccgaggc gctggacgca gaactggacg
    14161 cgctggaatc ggagatgccg aacatccagg cgcgcgccgc caacatgttc gtgctcgcag
    14221 gcgcatgggc cgagcgccac gacgccatcg tcgcggccgc accgccggcg gccctcgaag
    14281 cggtggaggc gcgcctgcgt cgcatcggca tccgctgggg catgatgccc ggcgcacgca
    14341 tgaccgcgca gttcccggcg ctgccagcgg ccggcgacac ctctacgccc tgacctagcc
    14401 cacgacgcag ccggcgcgcg cgagcctcgc ggctacccgc gaaagccaag gcaaggtggc
    14461 ttagccctca ggcgcccagc aattccacgt cgaacttcag cgtggcattc ggcgggatga
    14521 cgccaccggc tccgcgcgtg ccatagccca ggctggcggg gatgatcagg gtgcgctggc
    14581 cgccttcctt catgccctgc acgccttcgt cccagccctt gatcaccatg ccgctgccga
    14641 gcgggaagat gaagggctcg cctcgatcct tgctggagtc gaacttctcg ccctgcgcgc
    14701 cgttttcgta cagccagccg gtgtaatgca cggtgacatt gacgccaggc ttggcttcgg
    14761 tgccggtgcc gacgacggtg tcttcgaact gcaggccgga ggccgtggtg gtgaatgcca
    14821 tgggaggttc cttgtggggc aagggaaggg ggcgtgcagt ctaccgggcg tgcggcttcc
    14881 ggccttcgca tcccggggct ggcaccattc ccgctccgca ccatgctgca gcccgatcgc
    14941 tgccgcatac tgcgtgcacc caaaccctgg tcccggaggc tgtcgtgttc gagcgatttt
    15001 cccgtagttg gggcctgatc aaggccagcg ccggcgtact ggccaaggac aaggaactgc
    15061 tggtgttccc gttgctctcg gcgatctgca cgctgatcgt ggcggccgca ttcttgttgc
    15121 cggccgtcgg cctgggcgcg ctggacggac tgcgcgagga cggcatgtcg gtcacggctt
    15181 atgtgttcgc gttcctgttc tacctggtgc agtacttcgt catcttcttc ttcaactccg
    15241 cactggtggg ggcggcgatg atccgcctgg agggcggcga cccgaccgtg cgcgacggcc
    15301 tgcgcatcgc cggcagcaag gtggtgccga tcctgggcta tgcggccatc gccgcgaccg
    15361 tgggcatggt cctgcgcgca atccaggaac gcgcgggctt cctcggccgg atcgtcgccg
    15421 gcctgatcgg cgtggcgtgg acgctggcca gcttcctggt ggtgccggtg ctggtctcgc
    15481 gcgacatcgg cccgctcgac gcggtgaagg aaagcgcact gctgctgaag aagacctggg
    15541 gcgagaacct gatcggccaa ggcggcgtgg gcctggtgtt cggcctggtc ttcttcgcac
    15601 tgatcatcgt gggcggggcg ggcatcatcg gcgcggcgac gaccgggaat ggcgtgctga
    15661 tcggactgat ggtcgccctg ctgatcgtgg cgctgctggt ggccgccctg gtgcaggcgg
    15721 cactgtcggg catctactcc gccgcgctct atcgctatgc ggtgggcgcg ggcgacagcg
    15781 aaggcttcaa cgcgcagttg ctggggcagg cgttccgcac caagtaagcg caggcgtcaa
    15841 gcgagggaat cgacgcatcg attccctcgc cgcacactgc agggtcattc ggcatcgata
    15901 tcgatgcgca tgccctgcac ggtgttctcg acgacggtga ccacgacgtc gcgctcgccg
    15961 tcctgcttct tcagcaacac gtcctcgccg tcgtcgacct cgacgttgta gtggttgctg
    16021 gccacggcct gcagggcggc gcttacatca cgggccacgt ggttttccgc gcgaccgttg
    16081 gccacctgcg ccacggcgcg gatcggctcg cccttggccg gtgccacctg caggatgcac
    16141 cgaaggcact gcccgtcgcg ccgatccccg gtgacggatc gcacaacgcc gggatgaacg
    16201 acacgccgcc atcgacaccg cccgcgcggt cgctcaccct gcgcgctggc ccgccgcgaa
    16261 cgcccccgat ggcacctgtc tccccgtcgc cgctccgcac cgccgtcctc gcgggtgcct
    16321 ggctgctggc ggccgccatc gccctgcatt tcctggcatc ggcgcatgcc agatacgcgc
    16381 aaccggatcc cgccgcctac ggcatgttct ggactcgccg ccactggctg tgggtgcacc
    16441 tggccggcgg cgtgctggcg atggtgctgg gcagcgtgca gttcatcggc ggattgcgca
    16501 ggcgctggcc acgcgtgcac cgttggatcg gcaggacgta cctgctcggc gtttgcatcg
    16561 ggctggtcgg cgccgtcgcg ctggtcgcga catccccggc ccccatcgcg atccgcatcg
    16621 ccttcaccgc catcgccgct gcgtgggcgg gcaccgcgag cgccggttac cgcgcgatcc
    16681 ggggcgggga cgtgtcgcgc catcggcgct ggatgatccg cgcttacctg gtgacgttgg
    16741 cgcctgcgct gttccgcctg tcgctgctgg tgcccggggt gatggcactc gcgtcgccgc
    16801 tggtcatgat ccccaccctg ctgtggctga gctggacagc gccgctggcc gcatacgagc
    16861 tggcgcgtcc tcgcaggggc cgttgatcct ccgcggatcc tcgcctgcgc gcacgttcga
    16921 catggccgaa cccgccgccc ctgcgcagaa cggcgggaca tacccgccgt tcgatgcatc
    16981 aggtgggtcg tcctgcccgc ctcagtcgcg gcaatagccc tcgcccggct tgaccgtcag
    17041 gcagtcctgc ttgccggcca ggtacttcag gccggtcgca tcgccactgg tgtgcagcgc
    17101 ggtttcggcc accccattgc gcatgaagcg caggccctcg gcggtgacgg agccgtcgaa
    17161 catgcccttg ttgtcgagat cccactgcat gtccagggtc acgcccccgc cgggcttggc
    17221 ggccaccacc aggtacatgc cctccacgcc ggtccagcgg cccaggtaat cggcctcgac
    17281 cttcgcgctg tcggccggcg tggctggcgt ggcaggcgcg gggctggtgt cctcgtccgg
    17341 tgcggcggcc ggggcgggcg ttgcgtcgac tgcggccgcg tcggctggcg cagcggcatc
    17401 cgcaggcggt tggcgatcgg aacaggcggt gagggcggca accgacagca ggagggcaag
    17461 cgtcttcatg gggatctccg atcgcggaac gtgcgcgcac cctacgcagc ggcctgtgga
    17521 tgcggcgtca cgcgcgacgg gccgcgactt cgacgtcgaa ctcatcgcgc atgcacgttc
    17581 gtgagcgcca acggaaccgt cactcccgcg cgcgcaatcg gatgcgatcc ggtgcatccg
    17641 gatcgctgcg gccttcgtat cgtcactacg cacgcccggc caagcgccgt gatggcggcg
    17701 cccctcaccc aaggagaaca ccgatgaagc agcacctcac cctgccgatc gccattgcca
    17761 ccgcgcttgc gctggcggcc tgcagctcgc ccgagacccc gccgcccgcc gaacccgccg
    17821 ccacggtcga aaccccggcc gcgccgatgc cggaagagac gatgcccgcc accccggcca
    17881 accccagcgt gggtggcgcc gcgatggatg ccggcaagga catcgtcacc aatgcctcgg
    17941 ccgcgccgaa cctcagcacc ctcgtcgccg cggtgaaggc ggccgacctg gtggcgaccc
    18001 tgcagggcgc gggtccgttc accgtgttcg cgccgaccga cgacgccttc gccaagttgc
    18061 cggccggcac cgtggacggc ttgctgaagc cggagatgaa ggccgacctc gccggcctgc
    18121 tgacctacca cgtggtcgcc ggcaacgtgg atgccgccgc gctcacccag cagatcgaag
    18181 ccggcggcgg caaggccacc ctgaccacgg tgcagggcgc gaccctgacc gcgatgtcgg
    18241 acggcgcggg cggcatcacc ctcaccgacg gcaagggcgg caccgcgaag gtgaccaccg
    18301 ccgacgtgcg ccagagcaat ggcgtcgtgc acgtgatcga cagcgtgctg atgccgaagt
    18361 aatcgcgtcc cgccttcctc gtggcgtgac gcgtgcgggc ggcttcggcc gcccgttttt
    18421 tcgcgcgcga ggtgcgtgcg ccaggcgtgc agtcagcatt gcgaagccgc ggctcacgat
    18481 tccatcaccc tccgagaacc ggtggcccgg catggtggcg tcctttcgca catgcaggag
    18541 ttcacatggg taccccattg cgcaacaaga ccatcgccat cctcgccacc gatggcttcg
    18601 agcaggtcga actcaccgag ccgaagcgcg ccgtcgagca ggcgggcgcg accacccgca
    18661 tcctgtcgat caaggacggc cagatccagg gcatgcacca cgacaagaag ggcgacacct
    18721 tcgccgtcga tgcgcaggtg gccgacgcca gcatcgatga ctacgacgcc ctgctgctgc
    18781 ccggcggcgt ggccaacccg gatgcgttgc gcctggacaa ggctgcggtg gatttcgtcc
    18841 gcgacttcac caacagcggc aagccgatcg gcgtcatctg ccacggcccc tgggtcatgg
    18901 tcgaggccga cgtggtccgc ggccgcaaga tgaccgcgtg gcccagcgtg cgcaccgacc
    18961 tgcgcaacgc cggcgccgag gtggtggacg aggaagtggt caccgaccgg ggcatcgtct
    19021 ccagccgcaa gccggacgac ctgcctgcgt tctgcaggaa gatcgtcgag gagttcgccg
    19081 aaggcaacca cgcggcgcgc cgttcggccg cgtgacggcc ggaaccgatg gatgcaaaag
    19141 gcccgcgtga tgcgggcctt tttttcgggt gttggacgca gacgacgccg tggttggcgt
    19201 tccggcaggt gcgaccgcga tgtgtggaac ccgcttcccc cactccggtc ttcagtggtc
    19261 ggccagcagt gccgcggtgt ccagcaggcg gatgccgggc cgctgcgcca gcttctcgcg
    19321 gagccagtag ccatgctccg cgccgaccag caccacgatc cgcttgcccg ggttctccgc
    19381 ggcggcgcgc tcgatggtcg ccaggaactc gccattccac gcgtcccagc cggcgcgttc
    19441 gccctcgccg accaggcctt cctgcagcgc gtgcttcgcg cgcatctgcg cgtcggtgac
    19501 cgcatcgttg acccgcgcgg cactggtcca ataggcgcgc aacggtgcgt acatcgattc
    19561 ggcatgcgcg gagaacgcgg cggacgccgc cgggttggtg gccgcgaact cgcggctggc
    19621 ctgcaggtac ggcttcagga tcgcatcgaa gcgcggctgc gccggctcca tcgggtagac
    19681 gcgatagcga tgcgcgtcga tcagcggata caccgcgtcg gggtactcca ccttgttcgc
    19741 ctcgggcgca cgctgcgcat agcgatcggg ccgcacctcg atgcacagca cgtccggcgc
    19801 cagccgcagg atgctgtcgc gcagcacctg cagcgagtac gccggcacct gcgcgtgcag
    19861 cgtgtgcagc gtggccagca ccgcgacttc agcgcggggg gtcttgggcg gggcggcctg
    19921 cgccgcggga agcaggccac acagcaacag cgccagcagg aatcgcatcg gtcagtccgg
    19981 tcaacgcggg ttctgcgcgc ggggagtatg cgatggcgaa cgacgcaccg gcctgcgccg
    20041 gaagtcatcc gcagcagagg agctggaaca cggttcgctc gcgagccgcc gggtcgcatc
    20101 gcgttacgaa cggagcgtca cctgcgccct ttgcctcgaa gccggcccgt ttggctcgac
    20161 gcaatcaggt gcaagcggac attgcggaag aagacgtcac agcagggttt ccgcaaccgg
    20221 cgtcagcaat gccggggatg cggggcaggc atcgcccaag cgtgcccggt aaggtgccaa
    20281 gtccggcatg tcgggcttgg gccgatccaa cgcgaactgc atgggaatga cggcctggca
    20341 ctcgatcgca accccgtcca cctcgatcac cgagaacggc cactgctgga tcgccgtgcg
    20401 cgccgcttcg tccagcggcc gcatccggcg ctcgagcttc tccggcgaca acaaggtgat
    20461 ctcgcccgcc gcgcccttgc ggtccacccg caccgcgtaa accagcgtgc cctccacccc
    20521 gcgcttgcgc gattcgagcg ggtagtgcgg cggcaccatc atggtaccgc ccggcttgcc
    20581 atccgccgtc atcggcggcg gtgccccgcc aacctccttg atccgcatcg cgaatccacc
    20641 gcccgccacc tgcagcactt ccgccgtgat ctgttgcgac accgggcgcg ccaacgtcct
    20701 gccctgccat gtcgcgggcc ggtaacgcca ttcgctcacc cgcttgcgca ccatcgcctg
    20761 cagcgcaggt gggagcgagg catccggggt cacattggcg ggaacgccat ccacgccgat
    20821 gtcggccttg aagcccagcg caatcagcgt cggcaccttg ggtgctgcgg gcacctgcgc
    20881 gaacgcgggc gtcgcgcaca gcgcggtgag caggcaagtc gtcaggcgtt tcatgcgcga
    20941 ccggatccat ggttcgacgg gagggtggca gtgtgccagt tcacgtggcg atggcgcctg
    21001 tcattcgtcc atgcaagcac gaacccggga attgcccgga cacgttctcc tcgtcgtgat
    21061 gcaagactca aggtacaagg acccctggcc cggctatcgg tcggacacgt cgacgctgac
    21121 cccggttgtt ctaagtgacg gaactcttca acgcagacac gatgtcgatc agccgcgttc
    21181 cgctcaggat gctcgcgcgt atgaactgct tctgcgccac tgacaggttt ccgcccagcg
    21241 cgtcgcgcac cgccatctgg tccagatcga attccaggcc gcggacgagg cgcttcacat
    21301 cgccctcggg aagtccgtag tcggagagga gatgggagat ctcgtcgaga acatccatgt
    21361 gctcatacgc caaggctgga gaacgcgcgc caattgaacc cgagcacggg tctcgccccg
    21421 gtgaaggcgg acggaagctg tgtttcctct agacaagtga aaggcgggac accctcgccc
    21481 caagcaagtg aacctgaccc catgagtttc attccgcgtc gcgcccgccc gccactggcg
    21541 cgcgcacggc attccagttg aacaggaagc tgcggtgttc cgcccaggtc tggccacgcg
    21601 cgatctcgcc gtccaggcaa acggacgtgt ggtacggccc gacaccgtcg ctggtgcgga
    21661 agcttgcgca cacgcggccg cgatccgatt tccagcgccc ggcttcgatg cggctgttgt
    21721 agaagctgcc ttccacgccg ccatccgggc gcagggccag ctgcatcggc tgcgtgtagg
    21781 gcaccgcagg atcggtggcg agatcgacca cccagtcgcc cgccaatccc ggctcggcgg
    21841 ccgacacagg cattgcaagg gatgccgcaa tgcaaagcag gggcaggacg cgcatgacgg
    21901 tgttcctcgt tgattgctcg cgactggtta cgggcatgcg cgcggtccgg atgcacgaag
    21961 cgctcgtccg tggctcgttt tcgcggatcg ccaaaggcgg gtggaagcct tgcttcttcc
    22021 ggacaagcga aaggcgggca tgcccgcctc aagaaaagtc gactctgacc cctgctttcc
    22081 tactactggc ataaatagta gctcgccatc attgtcgtct tgctcgcttg ggagacatat
    22141 gcactgctac cgatcggaat gacagtagcc ggacctacct tcgtgttgga gttcaaaagc
    22201 atcaccgcgt ttgcgccacg tgaagccgct ccagtttggt gccactcaat gcacttctcc
    22261 gcacctcgac cggttttaca catctgctgg tcgacgtatg cgcaattttc ggcctgagtc
    22321 tccgttatca cagcaacctt cgttgggatg agataggggc gaccttccgc agactgtttc
    22381 tccagtattg cctttgcgtc ctttgcatgt cttgcaagtt ttttcacggg ggactgcgct
    22441 gcaagcagaa tcacgggcat atatgtttca tcgcccgacg cgcttaatgc tttgacatgc
    22501 caggcaagct cttcgacgcg aggcgaatct ttgtcaaggc ttggtagtgc actcccaatg
    22561 attgatgcaa ggaggtcatt tagccccttg tcattgatac cagaaccaaa tatttgcctc
    22621 gacgcctcca tcctcgctgc cattgagctc gactgaagct gctcagttag gtgccgcgcg
    22681 cggtctttgg acgcgaactg agcatgcgca ccagacgcga cgaacaaaag agaaatgaaa
    22741 aaccaaatcg ctactcggtt catttcaaac cctcaatcaa aaaaactgtc tccgaaaagt
    22801 cgactctgac cctgattttg gtgtttttac gatcccgttg ccgctgccgc agctcagagc
    22861 gagacgccct cgaccttcgt gtccagcacc ggtggcaacg gacagccatc gggaacgcgc
    22921 ggcagataag gcctcatgtc cggcatcgtc gctggcggca gcgaccggcc aacacccaga
    22981 cggaacgtga tcggcgtgat caggcggcag tcgatgcgct ggccgtccac ctcgaccggt
    23041 tccagttgcc attgcccgat cgccctgcgc gcggccgcat cgaacgtctt tttccagtta
    23101 ttgcctgcct gcgaatccag cagctccaca tcgctggtct tgccttgcgg atcgcgccgg
    23161 atcgcataca ccagagtttc ctcgacgccc tgccgctgcg cctctatcgg ataggaagga
    23221 ggattcatcg cacggctgga tggtgccccc ttgctgtcga gcaccatcgg tacgctggtc
    23281 acgcttttga tccgcagcga aaacccatcg ctcgccgctg gcaccacatc caccacaatg
    23341 cgttgcgaca cgtgccccgg caccggcttt ccctgccatg tgccggggat gtagcgccaa
    23401 cctgccacct gcttgcgcac caatgcctgc agtgccggcg acaagcccgg gtccggcgcg
    23461 atgttgaccg gccgcccgtc cacctgcacg tcggcctgga aatcgagcga gagtgcgcgc
    23521 ggcggcgatg ccgcgcgtgc ggggaagcct gcgcctagca cggcggccag tcccatcgaa
    23581 gccaagcggg cggcgtcaat tcgattcgat gaggctattg agcgcaccgt gggtcccctt
    23641 cagcacctga tagatgcagg aaagttggtc ggcttgcgcg ggtgtccacc ccgctcgcct
    23701 ggtgccgacc agtgcggcca gcctgaccga tgcagccttc gcctcttgca tgcgcgaata
    23761 cgccgcttgc gggatcttgc ctgacatcga tgcattcgtt tcgagcatgc tgttcagcgc
    23821 cgcgtgcgtg cccctcagca cctgatagat gcacgagagt tcatcggtct gggcttgcgt
    23881 ccatccggcc cgcttggtac caacgaggga ttgcagcctg tcggatgcgg ccttggcctc
    23941 gcgcatgtgg ggaaaggcgg cctgcgggat cttgaccggc agcgacgcat tcgtttcgag
    24001 gatgctgttc agggccccgt gcgtgccctt caggatctgg tagatgcacg agagctcgtc
    24061 ggtttgggcc tgcgtccacc ccgcccgctt ggcagccacc agggacacaa ggcgttcaga
    24121 cgctgccttt gcttccctca tttgggcata cgcccgatca ggaatcttcg acatattccc
    24181 tccacgcaat cgcgcagggg ttcgattgga acagaaaaag gagaccgagg gaatcaaggc
    24241 accctggttc cctccgtccc tttttattcc tggacaagcg aaaggcggga catgcccgcc
    24301 tttgaaggaa gtgaacctga ccccgttatc gtttgtcaac gtgccgctgc cgatggtgct
    24361 cttcaacagc gctcgcaacg cgacggtaca cgcggacccg aagggcggcg gtcaggacga
    24421 ccgccgtttt tcgatgggac aaggatgtcc catcgaaaaa tcccggcgca ggttgcagtg
    24481 cgacgcaggg ttggatcttc aggccatttc ttttggtgac ttttctttgg gccagcaaag
    24541 aaaagttacc cggccgcgga gcgggcggaa gctctgtttc atccagacat gcgaaaggcg
    24601 ggacatgccc gcccttggaa attgtctctg acagacttta acccttccgg ttaaccacca
    24661 ccgagaagct tcatggccat gcctccgatc tcttttagtc gggaggcagc ggtcaaaaag
    24721 gagtccgcga gaattaccgg tgcgcaaact tcactgaacc tatcccatac cttcttgagt
    24781 gctgaaaccg ctacgtcatc gctattttgc ttgatcaagg cttcattctt gattgcttcg
    24841 gtgtaggcag ctcgcacgtt gcgatgaaat tcatcctcgc cgcccacggc tccatcattt
    24901 atcgctcttc gaagcagctg gattatcgat acgagggctt ctgctagatc ggcattgctg
    24961 gaatttcttg ccagctgttc tgcctgatca agcaggtccg ctacttcatt gagcctggaa
    25021 tccgcaaggt ccacctcgtc gcacggaagc agctcatccc aaaactccaa ggtcttgaga
    25081 agatcttgag tcaattcatt cctcaagttt cctgcaggcg cctgaagact cgagaggcag
    25141 ttcgccaaga tcaagcgcaa tcggtgagct tgattgcttg tcgtgttcgg cgtgagtgtc
    25201 ggatgctcat ttctcagtcg ccgctcgacg agatcaactt gccttcctac taggctcagt
    25261 ttcctagcaa cctctgtggc aaccagttgt ggctgactcg catcaatgga ggagacgcca
    25321 aatacgcggg cccattgctg atggagcggc ttgttgttgg agaagctatt gaggtgttca
    25381 aagtaggtcc tcagtcgctt tgcagcactg gtctttctcg ctaattgcac gtgcgagcca
    25441 ctttcaccct cagaactcat cgcgacctat ccgcacttgc tatacccaca attcaaacaa
    25501 gtcgcacacc cgtccatcag gaccagcgcc ttggtcgaac acttgtggca catcgtggcg
    25561 gacggcggga aactcgcgcc gtcgccggtg acttcgatgt cttcggcatg gtcggcggtg
    25621 gacgagccca tcgcgccgcc catctcgccg gcggagacta cgtcagagtt tttttttgag
    25681 cggccttcga acgcgcggcg cttctcggcg atcagcgcgc gggtgtgctc gctcatctcc
    25741 gggtcgtgca tcatcccgat cgacttcagg tggtcttcca tgatcatgcc gagctcggcg
    25801 accagcgacg gcatgtacac gccaccggcc ttgaagtagc cgcccttcgg gtcgaacacg
    25861 gccttcattt cctcgaccag gaaggtcacg tcgccgccct tgcggaacac cgcggacatg
    25921 atccgggtga gcgccacgat ccactggaag tgctccatcg acttggagtt gatgaagacc
    25981 tcgaacgggc gacggtgttc gtggtcggtg ccggcgttga gcacgatgtc gttgatggtc
    26041 acgtacatgg cgtgctccac cagcggcgac ttgatcttgt aggtgctgcc gatcaggact
    26101 tccgggcgct cgatgcgctc gtgcatctgg atgacatcgg cgaccggcag ttcggcctcg
    26161 acctgggcgc gggtgacgga gtcggccttg accgcgtcct tcgccttgtc ctcgggggtg
    26221 acgacggcgt agcccttgat tttcttttcg atcctgacgg ccatgtgtat tccggttccg
    26281 gtgctgtctg tgtgcgtggg tgggtgctgg ccctcacccc aacccctctc ccgcggacgg
    26341 gagaggggct cgagcgggtc acttcttctt tgcagccttc ttggcggcct tcttggcagg
    26401 cgccttcttg gccgcggcct tcttcatcgg ggctttcttt gccgccgcnn nnnnnnnnnn
    26461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26521 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg gcaggcgcct tcttggccgc ggccttcttc
    26581 atcggggctt tctttgccgc cgctttcctg gcgggcgctt tctttgccgc cgctttcctg
    26641 gcgggcgctt tcttcgctgc cgccttcttg gcgggcgcct tcttcgccgc tgccttcttc
    26701 gctggcgcct tcttgaccgc cttcttcacc gccgccttct tggcggtggc cttcttggcg
    26761 accttcttgg tggccttctt cgccgcggtc ttcttgccgg cgaccttctt cttcaggctg
    26821 gccacttcct tcttggcatc tgcgctggcg gcggcgagct tcttcttcgc gttcgccacg
    26881 gtcttcttga ccgccttgct ggctttctcg gtgcgcttgg ccacggcctt cttggcgttg
    26941 gcgaccttgg acttcaccga cttctccgcg gacttggcgg ccttcttcgc cttggccacg
    27001 gtggcgctgg cggtgctggc cacggcgtcg ccgatctctg acgctttttc cttcacgctt
    27061 tctgcggcat cggacaacgt ggccgccact ccatttccat tgctcatgaa gccctccttc
    27121 gtcgggcgtt ggttggtatt acagcgcgat cctatacctg cttgcggcgt gcgtgcagcg
    27181 tgacggattg caactgccgc gcccgccgca gggcgcggtc agaacttgcc gtgagccttc
    27241 ctgcaatgca ccaccgcaag cccctcagaa cttgccgtaa tagccttcct tcaacgcgtc
    27301 gaagaggttg gccgcggtgt gcagttcgcc gtcgtattcg atctgctcgt tgcccttgac
    27361 ctcgaccacg ctgccgtctt ccagctcgaa gcggtaggtg gtgttctcca ggtcggcgtc
    27421 cttgaccagc acgccctgga aggcggccgg gttgaagcgg aaggtcgtgc agcccttcag
    27481 gccctgctcg tgggcgtagc ggtagatgtc cttgaagtcc tcgtacgggt agtcggtggg
    27541 gacgttcgcg gtcttggaga tcgaggaatc cacccacttc tgcgcggcgg cctgcacgtc
    27601 cacgtgcgcc ttcggggtga tgtcgtcggc gctgatgaag tagtccggca gcttcgacgc
    27661 ggcgtcctcg gcgtacggca tcgccttcgg gttgaccagt tcgcggtagg ccagcagctc
    27721 gaacgaccag acgtcgacct tttccttggt cttcttgcct tcgcggatca cgttgcggct
    27781 gtagtggtgg gcgaagctgg gctcgatgcc gttgctggcg ttgttggcca ggctcaggct
    27841 gatcgtgccg gtcggcgcga tcgagctgtg gtgggtgaag cgggcaccga cctcggccag
    27901 ttccgcgacc aggtccgggg cgacctgggc cacgcgctgc atgtagcggc tgtacttcgc
    27961 gtgcagcacc ttgccgctga tctcctggcc gaccttccag ccgtccttcg ccatctccgg
    28021 gcgcttgcgc agcatctcgg cggtgacctc gaacacctcg tccatgatcg gcgcggcgcc
    28081 cttttccttc gccagcgaca ggcccatctc ccagccggcc accgccatgt cgcgggcgat
    28141 gcgctcggtg aactcgcagg atgccgcact gccgtacttc atctgcagca tggtcacggt
    28201 gctgcccagg cccaggaagc ccatgccgtg gcggcgcttg cgggcgatct cgtcgcgctg
    28261 ctgctgcaac ggcaggccgt tgacctcgac cacgttgtcc agcatgcggg tgaacacgcg
    28321 gacgacttcc ttgtattcct cccagtcgaa gctggccttg tcggtgaagg gatgcctgac
    28381 gaacttggtg aggttgatcg agcccagcag gcaggccccg tacggcggca gcggctgctc
    28441 gccgcaggga ttggtggcgc ggatggtctc gcaccaccag ttgttgttca tctcgttgac
    28501 gcggtcgatc aggatgaaac cgggctcggc gtagtcgtac gtcgagacca tgatcatgtc
    28561 ccacaggtgg cgggcgcgga tctggccgta gatcttgcag gcgaccaggc cgtcttcgcg
    28621 gctgatgtag ccggcgtggg tgggccactc gcgccagacc acctgctcgg cattggccag
    28681 gtcgatctcg tcggcttcct tctcggccac cgggaacacc agcggccagt cgctgtcgtt
    28741 cttgaccgcg tccatgaagc cgtcggtgat cagcagcgac aggttgaact ggcgcaggcg
    28801 gccgtcttcg cgcttggcgc ggatgaagtc cttgacgtcc gggtgggcga tttcgaaggt
    28861 gcccatctgc gcaccgcggc ggccaccggc cgagctcacg gtgaagcaca tcttgtcgta
    28921 gatatccatg aaggacatcg ggccgctggt gtacgcgccg gcaccggcga cgaacgcgcc
    28981 gcgcgggcgc agcgtgctga attcgtagcc gatgccgcag cccgccttca gggtcaggcc
    29041 ggcctcgtgg accttctcca ggatcccgtc catcgagtcg gtgatggtgc cggacacggt
    29101 gcagttgatc gtgctggtcg
//
LOCUS       DAOOCX010000014        27971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1356_length_27971_cov_7.918756, whole genome shotgun sequence.
ACCESSION   DAOOCX010000014 DAOOCX010000000
VERSION     DAOOCX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 27971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27971
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1356_length_27971_cov_7.918756"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..1247
                     /locus_tag="PK399_02490"
     CDS             285..1247
                     /locus_tag="PK399_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HPW12028.1"
                     /translation="MARISALVEDVRRVLRTRHYSSRTETVYLGWIKRFILANRLRHP
                     SEMGQREVEAFLTRLAVEGQVASSTQNQALSALLFLYRDVLRVRMDWMEEVVRAKRGR
                     KLPVVLSGEEIRLLLAQLDGRAWLLASVLYGTGMRLMECLRLRVKDVDFARGEITVRD
                     GKGGKDRRTVLPRSLVEPLQLEVERARILHASDLAAGFGAVWLPHALARKYVNGPREF
                     GWQYVFPARNRSLDPRDDTERRHHVDAEFVSRALKQARNRAGIHKPVTAHTLRHSFAT
                     HLLEAGYDIRTIQELLGHKDVATTQIYTHVLNRGPNAVRSPLDR"
     gene            1354..2277
                     /locus_tag="PK399_02495"
     CDS             1354..2277
                     /locus_tag="PK399_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HPW12029.1"
                     /translation="MGLACRRRHLSRSTEAAYRYWARRLLEFLDGQALETVAVVRFLD
                     HLASVERVAASTHLQALHAALFLFRHVLGREVGRLEGLRTMRRPARLPVVLSVEEVRA
                     VLNAMHGMPRLVAALLYGSGLRVGEALALRIKDIDFRAGTITVRAGKGAKDRVAILPG
                     RLRDSLHRLALWRLALHKRDVMQGGGHAPLPDALERKYPSASRAFGWQFLFASSAVRP
                     CPRTGHRLRWHLSPSTVQEAFHEALRAIGCHKQASLHVLRHSFATHLLAQGADIRTIQ
                     QLLGHRHLDTTMIYTHVHQPAQGTVSPLDRF"
     gene            complement(2301..2690)
                     /locus_tag="PK399_02500"
     CDS             complement(2301..2690)
                     /locus_tag="PK399_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12030.1"
                     /translation="MPRIVVALLLSSLLGACAYPYNVGVKATDGTTVALMPGQSASLA
                     DGLLRYVRLANDSRCKPDVQCVWAGDAVIELHWVPATGAPRDLSLHLNPQAGPSTARL
                     DTREVTFTHLARPQPQASLRIDLARDP"
     gene            complement(2753..3472)
                     /gene="nfi"
                     /locus_tag="PK399_02505"
     CDS             complement(2753..3472)
                     /gene="nfi"
                     /locus_tag="PK399_02505"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465601.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HPW12031.1"
                     /translation="MTDALPDWTGDVAALRAVQAELAQRVVLRDDFAKPLRTVAGFDV
                     GFEDGGAITRAAAVLLDAESLDVIDQQVARLPTRMPYIPGFLSFRELPALLEALAMLS
                     TAPDLAFIDGHGIAHPRRLGIAAHFGVATGLPSIGVAKKILTGEPRMALHEMRGAFTP
                     LRSGKDQIGWLLRSKPGCLPLIVSPGHRVAMPSAPELVMRYVGKYRLPEPTRLADRLA
                     SRRDPGMRDDDSVALFAGDDG"
     gene            complement(3472..4002)
                     /locus_tag="PK399_02510"
     CDS             complement(3472..4002)
                     /locus_tag="PK399_02510"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPW12032.1"
                     /translation="MPRLLASIALALTLAGCATTPRTPDTTSVTFIVIRHAEKVDASR
                     DPDLSPAGHARAQALAASLGERELAAAYATEFKRTSQTLAPTVQARGLAITPYPAGQP
                     ATEFAARLRAAHPRGTVLIAGHSNTVPDIVAALCACAVAPMADYEYDRMSIVRLDADG
                     SARLDVTRYGAPSPAP"
     gene            complement(4019..7510)
                     /gene="mfd"
                     /locus_tag="PK399_02515"
     CDS             complement(4019..7510)
                     /gene="mfd"
                     /locus_tag="PK399_02515"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPW12033.1"
                     /translation="MPDPASPLSFPVPLPKGGQSRAWWRAPSSGTALAAAIAAAAQRH
                     AGPLLVVARDNHAAHQLEADLRLLAAGNDGLPVLGFPDWETLPYDRFSPHPDIVSQRL
                     ATLARLPGLQRGIVVVPVATLMQRLAPLGHVLGNRFDLRSGQRLDLDAEKRRLESAGY
                     RNVPQVQDPGDFAVRGGLLDVYPMGSDAPFRLELLDDEIDTIRVFDPESQRSLERIDG
                     IELLPGREVPLDDAHVQRALDALRERFDIDTRRSPLVQDLKAGVAPAGIEYYLPLFFE
                     TPRGAESSTATLFDYLADGMLPLLCDGVGEAAEHAWQQIGQRYEQLRHDVERPLLPPP
                     QVWLSPDALREVLNRGGRIEVCAPQHPRQADAQPLPVQPAPALPLAAKDHAPAQALRD
                     FLGSYPGRVLVAADSAGRREALREVLEAAGLQPAALQGWQDFATHDARFALAVAAFDD
                     GFALDNPALCVLTERQLFPERASQPRRRKRAGREPEAIIRDLGELSLGAPIVHEDHGV
                     GRYRGLVAMDIGGMPGEFLDIEYAKGDRLYVPVAQLDRISRYSGANPDTAPLHNLGGE
                     QWAKAKKKAAEKVRDVAAELLEIQARRHARKGLAIDVDRAMYEPFAATFPFEETPDQH
                     AAIEAVIRDLASSQPMDRVVCGDVGFGKTEVAVRAAFVAATSGKQVAVLVPTTLLAEQ
                     HYRNFRDRFADWPLKVEVLSRFKSKKEIDAELQKLAEGKLDVIVGTHRLLQPDVKFKD
                     LGLVIVDEEQRFGVRQKEALKALRANVHLLTLTATPIPRTLNMAMSGLRDLSIIATPP
                     ANRLAVQTFVHAWDDALLREAFQRELARGGQVYFLHNDVESIGRMHDQLHALVPDARI
                     GIAHGQMPERELERVMLDFHKQRFNVLLCTTIIESGIDIPNANTIVIHRADKFGLAQL
                     HQLRGRVGRSHHRAYAYLTIPDQRSITSDAKKRLEAIASMDELGAGFTLATHDLEIRG
                     AGELLGEGQSGQMAEIGFSLYTELLERAVKSIKQGKLPDVDGDTRHGADVDLHVPALI
                     PDDYLPDPHARLTLYKRISAARDSEALRELQVEMIDRFGLLTDPTRHLFATAELKLDA
                     TRLGIRKLDLGATGGRLQFVEKPNVDPMSVIRLIQGQPKHYKMDGPDKLRITLDLPDA
                     VTRFNAARGMLTALAPG"
     gene            complement(7598..8188)
                     /locus_tag="PK399_02520"
     CDS             complement(7598..8188)
                     /locus_tag="PK399_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW12034.1"
                     /translation="MAARNRMPPWHEKILLANGREVLVRPIRPEDAVPLRAAFVLLEP
                     DEVRQRFLHPMKELGVEQAERLTRPDPRREFALVAAEPLPPGEALVGAVARIAIDDNG
                     RDAEFAILVSHYVNGMGLGRHLMRRLVRWAKGRKVLRIYGEVLESNLPMQALAASLGF
                     QREYSGDPGMIRMVLELPPPKPKLPPPLPGMAAASR"
     gene            complement(8271..9215)
                     /locus_tag="PK399_02525"
     CDS             complement(8271..9215)
                     /locus_tag="PK399_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HPW12035.1"
                     /translation="MPSSFFALFDDIATLLDDVSVMTKVATKKTAGVLGDDLALNAQQ
                     VSGIHAERELPVVWAVAKGSLVNKVILVPAALGVAALEAWLRGRGYNVPLITPLLMIG
                     GAFLCFEGVEKLAHKWLHKPQEDEQHHAELVGAVNDESVDMVAFEKDKIKGAIRTDFI
                     LSAEIIVISLGTVATQPFAQQFAVLAAISLIMTVGVYGLVGGIVKLDDLGLRLAREGA
                     GKLRTAIGNGILAFAPKLMKFLSIAGTAAMFLVGGGILVHGVPALHHLAETVRAYPPG
                     WLWENLYNAVVGIIAGALVLGVVAGIARLRGKPANPAH"
     gene            complement(9307..10164)
                     /gene="rlmJ"
                     /locus_tag="PK399_02530"
     CDS             complement(9307..10164)
                     /gene="rlmJ"
                     /locus_tag="PK399_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002802763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HPW12036.1"
                     /translation="MNYRHAFHAGNHADVLKHVVLLALCDALTAKPAPCFVLDTHAGR
                     GLYKLDGESAQKTGEAQDGIARLVAEGPRDPALQRYLNAVRACRQQHGAQSYPGSPWL
                     LAHALREQDRIAACELLPEEATVLSDTFARDPRVAVHQRDGYAAIKAMLPPKHGTTRF
                     ARGLVLIDPPYEAQLDEFDIAIKALREGLARWPQGMFALWYPIKQGRALQAFQRKAAA
                     IEAKSALVLELLVRPDDSPLRMNGSGMLILNAPWQFDVAMRPAMAALQAALGESGAAS
                     RVAWLRPPA"
     gene            10226..10903
                     /gene="creB"
                     /locus_tag="PK399_02535"
     CDS             10226..10903
                     /gene="creB"
                     /locus_tag="PK399_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HPW12037.1"
                     /translation="MPRMPTILLAEDEPAIADTVLYALRAEGFTAEHVLLGGSVLPRL
                     KLGGIDLVILDVGLPDRSGFEVCRDLRTTSAVPVVFLTARNDEIDRVLGLELGADDYM
                     AKPFSPRELVARVRARLRRHEGGETQRKGRFEVDAVAHRIRYAGHWLDMTRYEHALLH
                     ELLRRPGAILSRAQLLDRAWEDALESGERTVDTHVKTLRAKLRALDPARDPIRTHRGL
                     GYSIDVD"
     gene            10922..12367
                     /gene="creC"
                     /locus_tag="PK399_02540"
     CDS             10922..12367
                     /gene="creC"
                     /locus_tag="PK399_02540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HPW12038.1"
                     /translation="MKIGLRVLLGYFLIVAVAALLLVNVFVQQVKPGVRQAMEDTLAD
                     TANVLAELASDDLLAGRIHEGQFAGRIRAMGQREIGARIWGQPKHAPSYRITVTDARG
                     IVVYDSAGRDMGRDNSRWNDVHRTLRGQYGARSTRDDPDDEMSSVMYVAAPIRDGQGR
                     IAGVLTVSKPNRSIQPFIERSQDVILRWGWVLLGSALLVGLLVAWWLSRQLAGLRRYA
                     DAVSAGQRASLPERGIEKPAGEFADLGRALESMRRQLDGKQYVEHYVHSLTHELKSPL
                     AAIRAAAELLESPLPDADRARFAASIGSQSRRMATMVDKLLALAEVEHRQRIERPESL
                     DIAALAREVADDAASHGQVAQVRVEVEAGEGLPDLLGDPFLLRRLLANLVDNAIDFSP
                     VGGVVEVRLQVAGDALAIEVGDRGPGIPDYALERVFERFYSLPRPDGGIRSSGLGLNF
                     VAEIAKLHGGEASLANRDGGGAIARVVLPIG"
     gene            12461..13813
                     /gene="creD"
                     /locus_tag="PK399_02545"
     CDS             12461..13813
                     /gene="creD"
                     /locus_tag="PK399_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HPW12039.1"
                     /translation="MRLAFKAIMVVVLTLAILVPLTMVRGTINERQAYREEAVEAVAQ
                     SYARAQGMAGPVLVVPYSEEVELEETDAKGVLRKRQTTVEGRWLYFPKTLTLDGKLLP
                     SIRKRGLHQVRVYELQGRLDATFDARIPEADPMRPRKIGTPWLDIGIADVRGLSGTPV
                     LKTGATSLPVLQGQQGRDEAGVHALLPDTAIVDGKLAFPLQFQFALRGTEALRVTPLA
                     DSNRITLASAWPHPQFNGDFLPRAPRVDGSGFRAEWDVSSLASNAQAQYRAGRSEKSV
                     EMDGIGLSLVEPVDLYSKLDRASKYGLLFVLLTFVGFFMFETIRQLPIHPIQYLLVGL
                     ALAIFFLLLLGLSEHIAFGIAYFVASVACIGLLGFYLAHVLRSRARGVGFAAMLGLLY
                     AALYGLLLSEDNALVLGAGLLFAILAAVMIATRKVDWYQVAARAPGPVRIDPTTDHVA
                     "
     gene            13850..14461
                     /locus_tag="PK399_02550"
     CDS             13850..14461
                     /locus_tag="PK399_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW12040.1"
                     /translation="MDDAITLECLSGAALLPHLDAVAALRIAVFRDWPYLYDGDAAYE
                     REYLAAYAKSPDSLVVLARAGDTVVGASTGIPLAQDSAEFQAPFLARGFDVSTVFYCG
                     ESVLLPAWRGRGIGHAFFDAREAHARALGGFAWTAFAAVDRGADDPRRPPHHRGNDAF
                     WGKRGYVRQPGMTMRLHWNETGIGDIDHPLTFWLRPLDEDTRT"
     gene            14458..15375
                     /locus_tag="PK399_02555"
     CDS             14458..15375
                     /locus_tag="PK399_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007962834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPW12041.1"
                     /translation="MKVAACKYPIGEPRDFAAFAARQAALLGEAKARGAQLAVLPEYL
                     SLELAAMFDDAIRGDLHASLAAIQPLRDDWLSLYAGLARELGIGIVAGSFLLDMGRGR
                     YRNRSDFFAADGGHLWQDKLRLTGFEKRTGVIDSGDALKVFDAGGVRAGIAVCYDSEF
                     PLPVRAQCEAGARLLLVPSCTDTDAGATRVRVGCLARALENRCFVAQAVTAGEAPWSP
                     ALDTNTGEAALIAPMDVGLPSDGMLVQTRGSEAWAIAELDFDALEASRAQAQVANDRD
                     WSAQYFPTVLRARLERAGDHETAAMLARA"
     gene            15446..17029
                     /locus_tag="PK399_02560"
     CDS             15446..17029
                     /locus_tag="PK399_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPW12042.1"
                     /translation="MKRLMLATALLVAGVAGAQEKVDLDMTGRIRAEAFNRSQVMATL
                     NHLTDEIGPRLTNSPSMAEANRWTGSKFTEWGLSNVHDEAVDDIGRGWSFNDAGVEML
                     SPRVMPLHALPKAWTPGTTGPVEGEAIFAKLQNKGDLEKWKGKLKGKVLFTDELRPYK
                     PNEKSDFSRHDHDSLEELLSYPLPTERSRDASMLAQFRERMEFGPLLNQFLIDEGVVA
                     TMSISSWNDGIVRVMGGGSRKAGESVGVPALVMMAEHYNTVMRALDRKETVKLRVNVD
                     ARFLDDANKPGYNTIAEIPGTGPNRNEVVMLGAHMDSWHTGSGANDNGAGVAVMMEAV
                     RILKAVGARPNRTIRVALWTGEEQGLIGSQAYVAKHFAAYPEPTDPAQKALPAAFRTN
                     KGKLVKTGDYEKITAYFNLDNGSGKIRGIYAQENLAIAPIFEEWLKPWNDVGATIVTQ
                     RNTGSTDHVSFDRVGIPGFQFVQDQLDYFSHVHHTHLDVQDHAVADDLKQASAIVASF
                     VYNAAQRPGKLPRKMMVED"
     gene            complement(17147..17620)
                     /locus_tag="PK399_02565"
     CDS             complement(17147..17620)
                     /locus_tag="PK399_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPW12043.1"
                     /translation="MQAARRYFAAVSRLGDGVAWYGLMGALIVLDGMAGLRASLHLAL
                     TGLVALILYKSLKRWTKRPRPFASDVRIRAWVAPLDEFSFPSGHTLHAVAFSLVAMAH
                     YPLLAWLLVPFTASVAASRVVLGLHYPSDVLAATGIGCALAGLSLWLVPGVSLFG"
     gene            complement(17688..18857)
                     /locus_tag="PK399_02570"
     CDS             complement(17688..18857)
                     /locus_tag="PK399_02570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPW12044.1"
                     /translation="MRYAIVTETYPPEINGVALTVQGLQHGLRERGHAVQLVRPRQAG
                     DTATTISDHTLLVRGAGLPRYPGLRFGLPATRTLRRLWQAQRPDALYVATEGPLGWSA
                     LRAARQLGIPAATGFHTRFDAYMRDYGMPWLAPVALRWMRHFHNSAQATLVPTRELQA
                     LLEAERFDNVVRLARAVDTGHFDPAHRDPVLRAMWGVGERGVAAIFVGRIAAEKNLAL
                     AVEAFRALQRQGPDARFIWVGDGPARDALQRDNPDFIFCGLQRGEALARHFASADLFV
                     FPSHSETFGNVTLEALASGVPTVAFDYGAARECLRDGLHGAAIRRTGQPARDDAAFIA
                     AVARIGSDEATRRRMRPSCREAVSALRPTQVAADFDHLLQSLAQRGHGHVPIAAL"
     gene            19080..19490
                     /locus_tag="PK399_02575"
     CDS             19080..19490
                     /locus_tag="PK399_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPW12045.1"
                     /translation="MGRPQPLMIQIATGDPRPIGKQIVDAVRMKIATGELGAGDQLPS
                     VRGLAQQLTINPNTVAKAYAELTTEGWLESRQGMGLYVATPRQRLSEDERERRLDDAI
                     GRFVNDVIPLGYAPDEVQARVAHEFQQLVPRKIA"
     gene            19519..20433
                     /locus_tag="PK399_02580"
     CDS             19519..20433
                     /locus_tag="PK399_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW12046.1"
                     /translation="MTDQHDYVIETRALSKRYGRKLALDNLDLRIPRGRIHAIVGANG
                     AGKSTLFRILMGFMPPTTGEARILGRDSQSLTPQDRARIGFVNEEHTLPNWMRVEQVT
                     TMQKHQYARWNQQAFDSVIGHYHVLPEQKVGQLSRGERAGFNLALALAQRPELLVLDE
                     PTLGLDVVAKRAFLESLLYSNAADDCTVIYCSHQMEEIERVADNLIILERGQLKHMSA
                     PEDFTARVSHWVADVPFKGPELHTVPGLLEVQRLDGLHHYLVLDQDDGFEHFLRASGA
                     RNVQSMPVGLDRAVNAFLTKNHAAPAAA"
     gene            20455..22275
                     /locus_tag="PK399_02585"
     CDS             20455..22275
                     /locus_tag="PK399_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12047.1"
                     /translation="MIDLLKGELLRFRLWAGIAAFLNIAVLGFLSRMVDLAQQPLVVY
                     QVFAAVYAVAGILLGLYQMGSYRKPNQWLNLLHRPLHRLRIAGALTGAGALLLLLAVA
                     VPIALVALYQDTMTARVVDLRHWMLPVAAWLIAVSGYLAGAYAMVGNRRYSFAAFLLP
                     NLFMYAQATGVAMLGVQCVVIAFLAAMLAIAFKPDPAAPSHNPAAVLAIALPVQLGAY
                     FLVWMLGYGVEMAWTVTGTHPLNMPAPPKGGYIEADRAEGKDMLLLGIEGSRDPEAAL
                     WREQIALSDVYTTYPLRNLPVRNSLTNLSPLEFADGDNNLFLVYSHDRARFVTRGLRD
                     QRRIGEMGVGEGQSAFPAPTMPFGAEYLYNANAAYQYDGEQQRIFERIRLPRGEVMAS
                     PPAPAGDNLLVLSDRAAYFYPGREAMNGLDLLQPLMRVAMPGQVGNLSRMDVVELLDG
                     YLVSYTYTWGVWSGELQQPFQQVLRVDGEGQAREVGRRALSIDLPSVYTNRNTWLSPV
                     LRAICLGAQDLFAAKDPLRAKPEPMPVQILWLAGVCCLLSLLGAVWLSGRQAHSPRGR
                     WAWVLLCGVVGLPALVSLWLLVPRREALPVAAPDAQPVAA"
     gene            22301..24373
                     /locus_tag="PK399_02590"
     CDS             22301..24373
                     /locus_tag="PK399_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPW12048.1"
                     /translation="MTSPFRHFCIVLALLAAPFVPKPASAAPGEGDLRSAIAAQRSLP
                     RAARLPRTAFLENRALVGVQLAPAGDAVAYLREQKDSRALWLLPVDGGKPRMLVGSTQ
                     ADQLLWSRDGRWLFLRASRVLTIVGIDGRTGVRVPLGGTERRRLMKVDPSQPAAVLLG
                     ERVRVGASERWRVVRMDARGKRTLLREDARWIHDVALDARGRIVALARFEGDHDALYR
                     VRADGTLRDVLRLQAMERLDLLAAPADGSLLMTGNPGSAAAGGDFRRVMRLDANDRLQ
                     TLHQDPRGEADLDDVVVDPRTQQPLAASYRSTVAATYGIGGAQGVVADIATRFPGRDI
                     GLQAGSGPSARWLVSERAATLRDPRWWLYDPRNRELRRILDDPGITAGTPDETALARK
                     VAIDYVASDGRRVHGFVLLPPGLDAARAPLVAQVHGGPISHFRTGYDGVAQFLANRGY
                     VVFQSNFRGSTGHGRAYTFASNGDYGNGRVQQDIVEGVRWLLGQGIGDARRVGIVGHS
                     FGGYSTLQGLTFQPELFKVGVAGAPPSDLGWGMRWLVQSGDQGDLPDRSLKHTLAALS
                     LDATDPATYARLHAQSPQANAMKMRRPLLIMAGGADRTVAIRDVIHYSATLKSLGRAV
                     TLLVEPGGGHSPVDPVPREAYLFAMETMLQRHLGGPVPEAPGQRLRAYLRENLRLAGP
                     EFAGFAKK"
     gene            complement(24459..24848)
                     /locus_tag="PK399_02595"
     CDS             complement(24459..24848)
                     /locus_tag="PK399_02595"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPW12049.1"
                     /translation="MTPDIHDYNARQSQADRAICDLLAEAIHRGLPEAEARIWHAHPV
                     WFLDGNPVVGYSKLKESVRLLFWSGQSFDEPGLRAEGTFKAAEARYTTAGDVDAALLQ
                     HWLGKARDIQWDYRSLVKRKGRLERLK"
     gene            complement(24853..26394)
                     /gene="ppx"
                     /locus_tag="PK399_02600"
     CDS             complement(24853..26394)
                     /gene="ppx"
                     /locus_tag="PK399_02600"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HPW12050.1"
                     /translation="MRTTQRRTPANRASAPRDGDMLAALDLGSNSFHMVVAQRVLGQL
                     RVVDRLRETVRMAEGLDGFGGMSAEAQARAIDCLSRFGQRIADIPPAHVRAVATNTVR
                     QLRAPAGFLVPAEAALGHAIEVVSGREEARLVYLGVAHAQPPKDDQLRLVIDIGGGST
                     ECIIGRGMQPLERESLQAGCIASTRRFFPNGRLSKKKWNSALTEITAEFQQFASTYRS
                     LGWDEAVGSSGTNKAIGEICAALKLTKGAITAEALPLVRERLLQADRIEDIDLPGLSG
                     DRRPIIAGGVLVLEAVFAALGLQRMQVSKGALREGVLYDLLGRGGADDPREVSVEALM
                     HRYGIDAAQAARVEATALRLFDQVAPAWTLDGDDRRMLSRAARLHEIGLAIAHSGYHG
                     HGAYILEHSDIAGFSQQEQRVLAALVRTHRRGIPKSAFDMIPDRLLANTRHVAALLRL
                     AVLLHRAHETDELPVLRLQADGERLQLALAERWLRARPLLRADLAGEPDDMLGLGVTL
                     KLAAE"
     gene            complement(26454..>27971)
                     /gene="ppk1"
                     /locus_tag="PK399_02605"
     CDS             complement(26454..>27971)
                     /gene="ppk1"
                     /locus_tag="PK399_02605"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPW12051.1"
                     /translation="APRSLPRIIRLPDQDGEGDQFHDFVFLSSVLSAFVHELFPGMKV
                     NGAHQFRVTRNSELIVDEDDVDNLALALRDELLGRGYLRAVRLEIDERCPQDIVDSLL
                     ANFDLPANAVYKINGPVNLNRVVQVYDLVQRPDLKFPAFQPRMPREVDAMFDLIQQGD
                     VLLHHPFDAFTPVLELIRQAAEDPNVLAIKQTLYRTGKESAIVDSLIQAARKGKDVTV
                     VVELRARFDEEANLGLADRLQDAGVQVVYGVVGYKTHAKMLLIVRREGKSLRRYVHLG
                     TGNYHSGTARAYTDFSLMTADPDIGNDVHLIFQQLSGLAAPLTLKHLLQSPFTLHKGV
                     LERIARETAHARAGRPGRIVAKMNALNEPQVMLALYEASQAGVKVDLIVRGACTLRVG
                     IEGISENIRVRSVVGRFLEHHRIWWFGNDGQPELYCSSADWLERNLLRRVETAFPILD
                     AECARRVHQEGLLNYLLDNQNAWEVQPDGSYAKCIPAAGEEPFSAQLSLLDGLYA"
BASE COUNT         4315 a         9121 c        10087 g         4448 t
ORIGIN      
        1 gctggattca ctcagtcacg actacaagaa cctgatctgg cgccgcctaa cgcctagtag
       61 cttgtgaacg agcgtgatag tggcgaacgt tcatttttgt agggcctgat cgaaggagaa
      121 atcctaccca ataaacaacc gcttaccgaa agaggatcag tgggtgccgc gtgcacgatg
      181 gcctgcaaat ccagcgtagg cgcgatcata tgctttatgt cgatgaatcc tcgagatatc
      241 cccgcctcgt ggcagagcca acctgggagg gcgatgagtc gcagttggcc aggatatcgg
      301 cgctggtcga agacgttcgc cgcgtgttgc ggacgcgaca ctactcatcc cggacagaga
      361 cggtgtactt ggggtggatc aaacgattca tcttggcgaa tcggttgcgg catccctcgg
      421 agatggggca gcgcgaggtc gaggcgttcc tgacgcggct ggcggtggaa ggccaggtgg
      481 cgtcgtctac ccagaatcag gcgttgtcgg ccctgttgtt cctgtaccgc gatgtcttgc
      541 gggtgcggat ggactggatg gaggaggtcg tccgggcgaa gcggggtcgc aagctgccgg
      601 tggtgttgtc gggagaagaa atacgtttgt tgctggccca gttggatggg cgcgcttggt
      661 tgctggccag cgtgttgtac gggaccggaa tgcgcctgat ggagtgcttg cgcctgcggg
      721 tcaaggacgt ggatttcgcg cggggggaaa tcaccgttcg cgatggcaag gggggaaagg
      781 accgccgcac cgtactgccc cgaagtctgg tggaaccgtt gcagcttgag gtcgaacgcg
      841 cccggatcct acacgccagc gatctggcgg ccggtttcgg ggcggtctgg ctgccgcatg
      901 ccctggcacg caaatacgtg aatgggccac gcgagttcgg gtggcagtac gtgttcccgg
      961 cacgcaatcg ctcgctggat ccgcgtgacg ataccgaacg ccggcatcac gtggatgcgg
     1021 agttcgtgtc gcgggcactc aagcaggcgc gcaaccgggc ggggatccac aagccggtga
     1081 cggcgcacac gctgcggcat tccttcgcga cgcatctcct ggaggcaggc tacgacatcc
     1141 gcaccatcca ggagctgctg gggcacaagg acgtggcgac gacgcagatc tatacccatg
     1201 tgctcaaccg cggtccgaac gcggtgcgta gtccgctgga tcgctgaggg gtcgccgggg
     1261 gcgtacgctg gtgtctttca cagcacggag gtgcgacggt gatggggtaa cgaagaagcg
     1321 ttggcgggaa cacgggggga gttgctggag caggtgggac tggcgtgccg ccggcggcat
     1381 ttgagcagga gtacggaggc cgcctaccgc tactgggccc ggcggctttt ggagttcctc
     1441 gacgggcagg cgctggagac ggtcgcggtg gtgcggttcc tcgatcactt ggcgtcggtg
     1501 gaacgggtcg ccgcgagcac gcatctgcag gcgttgcacg cggcgctgtt cctgttccgg
     1561 cacgtgctgg gtcgcgaggt cgggcgcctg gaagggttgc gcacgatgcg gcggccggcg
     1621 cggttgccgg tggtgctgtc ggtcgaggag gtgcgtgcag tgctcaatgc gatgcacggg
     1681 atgccgcggt tggtggcggc gttgctgtat ggctcgggcc tgcgcgttgg cgaagcgctg
     1741 gcgttgcgga tcaaggatat cgacttcagg gcgggcacga tcacggtgcg ggccggcaag
     1801 ggcgcgaagg accgggtggc catcctgccc gggcgactgc gcgattcgtt gcatcgcctg
     1861 gcgctgtggc ggttggccct gcacaagcgc gatgtcatgc aaggcggtgg gcatgcaccg
     1921 ctgccggatg cgctggagcg aaagtacccg tcggcatcgc gggcgttcgg ctggcaattc
     1981 ctgttcgcct cgagcgccgt gcggccatgc ccgcggaccg gacatcggct gcgctggcac
     2041 ctgtcgccgt cgacggtgca ggaggcattc cacgaggcct tgcgcgcgat cggttgccac
     2101 aagcaggcgt cattgcatgt gctgcggcat tccttcgcca cccacctgct ggcgcagggc
     2161 gccgacatcc gcacgatcca gcagttgctg gggcatcgcc acctggacac gacgatgatc
     2221 tacacccatg tccaccaacc ggcgcaggga acggtcagtc cgctcgatcg cttttgacgg
     2281 ccaggtgcgt cgagtactcg ttacgggtcg cgggcgagat cgatgcgcag gctggcttgc
     2341 ggctggggac gggcgaggtg ggtgaaggtc acctcgcgcg tatccagcct ggcggtcgac
     2401 ggccccgcct gcggattcag gtggaggctc aggtcgcgcg gcgcgccggt ggccggcacc
     2461 cagtgcagtt cgatgaccgc atcgccggcc cagacgcact ggacgtcggg cttgcagcgg
     2521 gagtcgttgg cgaggcggac atagcgaagc aggccgtcgg ccaggctcgc ggactggccc
     2581 ggcatcaggg cgacggtggt gccatccgtc gccttcacgc cgacgttgta gggataggcg
     2641 caggcaccaa gcaggctgct cagcaggagg gcgacgacga tgcggggcat ggcggcaatt
     2701 cctgtgggag acggccggag cctagcgtcg gcggcatgaa tgcggcgcga gtctagccgt
     2761 catcgcccgc gaacagagcg acggaatcgt catcgcgcat gcccgggtcg cggcgcgagg
     2821 ccaagcggtc ggcgaggcgg gtgggctccg gcaggcggta tttgccgaca tagcgcatga
     2881 ccagttccgg tgccgagggc atcgcgacac ggtggccggg cgagacgatc agcggcaggc
     2941 agccgggctt gctgcgcagc agccagccga tctggtcctt gccgctgcgc agcggggtga
     3001 aggcgccgcg catctcatgc agcgccatcc gcggttcacc ggtgaggatc ttcttggcga
     3061 cgccgatgct gggcaggcca gtggccaccc cgaaatgcgc ggcgataccg agccggcgcg
     3121 ggtgggcgat gccgtggccg tcgatgaagg cgagatcggg cgcagtggac agcatcgcga
     3181 gcgcctcgag cagcgccgga agttcccgga aactgaggaa ccccgggatg tagggcatgc
     3241 gcgtgggcag gcgtgcgact tgctggtcga tgacgtcgag cgactcggca tcgagcaaca
     3301 cggcggcggc gcgggtgatg gcgccgccat cctcgaaacc gacgtcgaag ccggcgacgg
     3361 tgcgcaacgg cttggcgaaa tcatcgcgaa ggacgacacg ctgcgcgagt tcggcctgca
     3421 cggcgcgcag ggcggcgaca tcgccggtcc agtcgggaag ggcgtcggtc atcacggcgc
     3481 gggcgagggc gcgccgtagc gggtgacgtc caggcgggcg ctaccgtcgg cgtcgaggcg
     3541 gacgatgctc atgcggtcgt attcgtagtc cgccatcggc gccaccgcgc aggcacacag
     3601 cgcggcgacg atatccggca cggtattgct gtggccggcg atcagcacgg tgccacgcgg
     3661 gtgggccgct cgcaggcgtg cggcgaattc agtcgcgggt tggccggcgg ggtacggcgt
     3721 gatggcgagc ccgcgtgctt gcacggtcgg cgccagcgtc tgcgacgtgc gcttgaattc
     3781 ggtggcgtag gcggcggcaa gttcgcgctc gcccagagac gcggcgagtg cttgcgcgcg
     3841 cgcgtggcca gccgggctca ggtcgggatc gcggctggca tcgaccttct cggcatgccg
     3901 aatcacgatg aaggtgacgg aggtggtgtc cggcgtgcgc ggtgtcgtcg cgcagccggc
     3961 aagcgtgagc gccagtgcga tggaggcgag gagtcgaggc atgcggcgaa gcatacgact
     4021 agcccggtgc caaggctgtc aacattccgc gcgccgcatt gaagcgggtg acggcatccg
     4081 gcaggtcgag ggtgatgcgc agcttgtcgg ggccatccat cttgtagtgc ttcggctggc
     4141 cttggatcag gcggatcacc gacatcggat cgacattggg cttctcgacg aactgcaggc
     4201 gcccaccggt cgcgccgagg tcgagcttgc ggatgccgag gcgggtcgcg tccagtttga
     4261 gttcggcggt ggcgaacaag tgcctggtgg gatcggtgag caggccgaag cggtcgatca
     4321 tctccacctg cagttcgcgc aaggcttcgc tgtcgcgggc ggcgctgatg cgcttgtaca
     4381 gggtgaggcg cgcgtgcggg tcgggcaggt agtcgtcggg gatcagcgcg gggacgtgca
     4441 ggtcgacgtc ggcgccgtgg cgggtgtcgc catcgacatc gggcagcttg ccctgcttga
     4501 tcgacttgac cgcgcgctcg agcagttcgg tgtacaggct gaagccgatc tcggccatct
     4561 gcccgctctg gccttcgccc agcagctcgc cggcaccgcg gatttccagg tcgtgcgtcg
     4621 cgagggtgaa accggcgccg agttcgtcca tcgaggcgat cgcttccagc cgcttcttcg
     4681 cgtcgctggt gatgctgcgc tggtcgggga tcgtgaggta ggcatacgcg cgatggtgcg
     4741 aacggccgac gcggccgcgc agctggtgca gctgggccag gccgaacttg tcggcgcggt
     4801 ggatcacgat ggtgttggcg ttggggatgt cgatgccgga ttcgatgatg gtcgtgcaca
     4861 gcagcacgtt gaagcgctgt ttgtggaaat ccagcatcac ccgctccagc tcgcgttccg
     4921 gcatctgccc gtgggcgatg ccgatgcggg catcgggcac tagcgcgtgc aactggtcgt
     4981 gcatgcggcc gatgctctcc acgtcgttgt gcaggaaata cacctggccg ccgcgcgcga
     5041 gctcgcgctg gaaggcctcg cgcaacagcg cgtcgtccca ggcgtggacg aaggtctgca
     5101 ccgccaggcg gttggccggc ggggtggcga tgatcgaaag gtctcgcagc ccgctcatcg
     5161 ccatgttgag cgtgcgcggg atcggggtgg cggtgagggt gagcaggtgc acgttcgcgc
     5221 gcagcgcctt cagcgcttcc ttctggcgca cgccgaagcg ttgttcctcg tcgacgatga
     5281 ccaggccgag gtccttgaat ttcacgtccg gctgcagcag gcgatgggtg ccgacgatca
     5341 cgtcgagctt gccctcggcg agcttctgca gttcggcgtc gatctccttc ttgctcttga
     5401 agcgcgacag cacttcgact ttcagcggcc agtcggcaaa gcggtcgcgg aagttgcggt
     5461 agtgctgttc tgccagcagc gtggtcggca ccagcaccgc gacctgcttg ccgctggtgg
     5521 cggcgacgaa ggcggcgcgc accgccactt cggtcttgcc gaagccgaca tcgccgcaga
     5581 ccacgcgatc catcggttgc gaggacgcca agtcgcggat caccgcctcg atcgcggcat
     5641 gctggtcggg ggtttcctcg aacgggaagg ttgccgcgaa gggctcgtac atcgcccggt
     5701 cgacgtcgat ggccaggccc ttgcgcgcat gcctgcgcgc ctggatctcc agcagttcgg
     5761 cggcgacatc gcggactttc tcggcggcct tcttcttcgc cttcgcccat tgctcgccgc
     5821 cgaggttgtg cagcggcgcg gtgtcggggt tggcgccgga atagcgactg atgcgatcga
     5881 gctgcgccac tggcacgtat aggcgatcgc ccttggcgta ctcgatgtcg aggaattcgc
     5941 cgggcatgcc gccgatgtcc atcgccacca ggccgcgata gcgaccgacg ccgtggtcct
     6001 cgtgcacgat cggcgcgccc agcgacaatt cgccgaggtc gcggatgatc gcttcgggct
     6061 cgcgcccggc gcgcttgcgg cgacgcggct gcgaagcgcg ctcggggaat agctggcgct
     6121 cggtgagcac gcagagtgcg gggttgtcca gcgcaaagcc gtcgtcgaaa gcggcgacgg
     6181 ccagcgcgaa gcgcgcatcg tgggtggcga agtcctgcca gccctgcaag gccgcgggct
     6241 gcagtcccgc ggcttccagc acttcccgta aggcttcgcg tcgcccggcc gaatccgcgg
     6301 cgaccagcac gcggcccgga taactgccaa ggaaatcgcg cagcgcctgc gccggcgcat
     6361 ggtctttcgc tgccagcggc aacgcgggcg cgggttgcac cggtagtggc tgcgcgtcgg
     6421 cctggcgcgg gtgctgcggt gcgcagacct cgatgcggcc gccgcgattc aggacttcgc
     6481 gcagcgcgtc gggagacagc cacacctggg gcggcggcag caacgggcgt tcgacgtcgt
     6541 ggcgcagttg ctcgtagcgc tggccgatct gctgccaggc gtgctcggcg gcttcgccga
     6601 cgccgtcgca gagcagcggc agcatgccgt cggcgaggta gtcgaacagg gtcgcggtac
     6661 tcgattcggc accgcgcggc gtctcgaaga acagcggcag gtagtactcg atgccggcag
     6721 gcgcgacgcc cgccttcagg tcctgcacca gcgggctgcg ccgggtgtcg atgtcgaagc
     6781 gctcgcgcaa cgcgtccagc gcccgctgta catgcgcatc gtccagcggg acctcgcggc
     6841 cgggcagcag ttcgatgccg tcgatccgct ccagcgaacg ctgcgattcc ggatcgaaca
     6901 cgcggatcgt gtcgatctcg tcgtcgagca attccagacg gaacggcgcg tcgctgccca
     6961 tcggatacac gtcgagcagg ccgccgcgga cggcgaaatc gcccgggtcc tgcacctgcg
     7021 gcacgttgcg gtagccggcg gattccagcc ggcgcttttc cgcatcgagg tccaggcgtt
     7081 ggccgctgcg caggtcgaaa cgattgccca gcacatggcc cagcggggcc agccgctgca
     7141 tcagggtggc gaccggcacc accacgatgc cgcgctgcag ccccggcagt ctcgccagcg
     7201 tggccaggcg ctggctgacg atgtcgggat gcgggctgaa gcgatcgtag ggcagggttt
     7261 cccagtcggg gaagccgagg accggcaggc cgtcgttgcc ggcggcgagc aggcgcaggt
     7321 cggcttccag ctgatgcgcg gcgtggttgt cgcgggcgac caccagcagc gggccggcat
     7381 gtcgctgcgc ggccgcggcg atcgcggcgg ccagcgcggt gccggacgaa ggcgcacgcc
     7441 accaggcgcg cgattgcccg cctttcggca gggggacggg gaaggacagc ggcgaggcgg
     7501 gatcaggcat gcgaaaaggc gaccggcgcc gggcgcgatg cgccagcgcg tggatgtcca
     7561 ggttgtggga acgcagggcg gagtttagcg gctcggctca gcggcttgcg gcagccatgc
     7621 ccggcagcgg gggcggcagc ttgggcttgg gcggtggcag ctccaacacc atccggatca
     7681 tgccgggatc gccggaatat tcccgctgga accccagcga agccgccagc gcctgcatcg
     7741 gcaggttgct ttccagcact tcgccgtaga tccgcaggac cttgcggccc ttggcccagc
     7801 gcaccaaccg gcgcatcagg tgccggccca ggcccatgcc gttcacgtaa tggctgacca
     7861 ggatggcgaa ttccgcatcg cggccgttgt cgtcaatggc gatgcgggcg accgcgccga
     7921 ccagggcttc gcccggcggc aggggttcgg cggcgaccag ggcgaactcg cggcgtgggt
     7981 cgggacgggt caggcgttcg gcctgctcca cgcccagttc cttcatcgga tgcaggaagc
     8041 gctggcgaac ctcatccggc tccagcagca cgaaggccgc gcgcaggggc acggcatctt
     8101 cggggcggat cgggcgaacc aggacctcgc gaccattggc gaggaggatt ttttcgtgcc
     8161 acgggggcat gcggttgcgt gcggccatcc gggcatcatg gcggcatcga agttaagaaa
     8221 atgaatggcg cgtgtccggc gggaccggac ggcgccgttt ggcggccggt tcagtgcgcc
     8281 gggttggccg gtttgccgcg caggcgggcg atcccggcga ccacgcccag taccagcgcg
     8341 cccgcgatga tcccgaccac ggcgttgtac aggttctccc acagccagcc cggcgggtag
     8401 gcgcggacgg tctcggccag gtggtgcagc gcgggcacgc catgcaccag gatgccgccg
     8461 ccgaccagga acatcgccgc ggtcccggcg atcgacagga atttcatcag cttcggggcg
     8521 aacgccagga tcccgttacc gatcgcggtg cgcagcttgc cggcgccctc gcgggccagc
     8581 cgcaggccga ggtcgtcgag cttgacgatg ccgccgacca ggccgtacac gcccacggtc
     8641 atgatcagcg agatggccgc cagcaccgcg aactgctggg cgaagggctg ggtggccacg
     8701 gtgcccagcg agatcacgat gatttcggcc gagaggatga aatcggtgcg gatcgcgcct
     8761 ttgatcttgt ccttctcgaa cgcgaccatg tccacggact cgtcgttgac cgcgcccacc
     8821 agttcggcgt ggtgctgctc gtcttcctgc ggcttgtgca gccacttgtg cgcgagtttt
     8881 tccacgccct cgaagcagag gaaggcaccg ccgatcatga gcagcggggt gatcagcggc
     8941 acgttgtagc cgcgcccgcg cagccaggcc tccagcgcgg ccaccccgag cgccgccggc
     9001 accaggatga ccttgttgac cagcgagccc ttggccaccg cccagaccac cggcagctcg
     9061 cgttccgcgt ggatgccgct gacctgctgc gcattgaggg caaggtcgtc gccgagcacc
     9121 ccggcggtct tcttggtggc gaccttggtc atcaccgaga cgtcatcgag cagggtggcg
     9181 atgtcgtcga acagggcgaa gaaactggaa ggcatgaggg ctcggggcta ggcaggagtg
     9241 gcgtcggcgc gcagtttcgc acgcgggcaa atgtgggctg gatgaagagt ggtgccgttt
     9301 cggtcgctag gcgggtggtc gcagccacgc gacccgcgac gccgcgccgg attcgcccag
     9361 cgcggcctgc aatgccgcca tcgcgggccg catcgccacg tcgaattgcc agggcgcatt
     9421 gaggatgagc atgccgctgc cgttcatccg cagtggcgaa tcgtcgggcc gcaccagcag
     9481 ctccagcacc agcgcggact tggcctcgat ggcggcggcc ttgcgctgga aggcttgcag
     9541 tgcgcggccc tgcttgatcg gataccacag ggcgaacatg ccctgcggcc agcgcgccaa
     9601 gccttcgcgc agggccttga tggcgatgtc gaactcgtcg agttgcgctt catacggcgg
     9661 gtcgatgagc accaggccgc gggcgaatct ggtggtgccg tgcttcggcg gtagcatcgc
     9721 tttgattgct gcgtagccat cgcgctggtg cacggccacg cgcggatcgc gggcgaaggt
     9781 gtccgacagg acggtcgctt cctccggcag cagttcgcag gcggcgatcc ggtcctgctc
     9841 gcgcagcgcg tgcgccagca gccacggcga gccggggtag gactgcgcgc catgttgctg
     9901 gcgacaggcg cggactgcgt tgaggtagcg ctgcagcgcg gggtcgcgcg ggccctcggc
     9961 gaccagccgg gcgatgccgt cctgggcctc gccggtcttc tgcgcggatt cgccgtcgag
    10021 cttgtacagg ccgcggccgg cgtgggtgtc cagcacgaag cagggcgcgg gcttcgcggt
    10081 cagggcgtcg cacagcgcca gcaggacgac gtgcttgagc acgtccgcgt ggttgccggc
    10141 gtggaaggcg tggcggtaat tcatgcgcgc attgtcgcat cgacttcaca ttcgccaccc
    10201 aaagcgcgtc ccgtggccgg ttacgatgcc gcgcatgccc accatcctgc tggccgaaga
    10261 cgaacccgcc atcgccgaca cggtgctgta tgcgctgcgc gcggagggtt tcaccgccga
    10321 acacgtgttg ctgggcggca gcgtgctgcc acgcctgaag ctgggcggca tcgacctggt
    10381 gatcctggac gtggggctgc cggaccgcag cgggttcgag gtctgccgcg acctgcgcac
    10441 caccagcgcg gtgccggtgg tgttcctgac cgcgcgcaac gacgagatcg atcgcgtgct
    10501 cgggctggaa ctgggcgccg acgactacat ggccaagccc ttttcgccgc gcgagctggt
    10561 ggcgcgggtg cgcgcgcggc tgcggcggca cgagggtggc gagacccagc gcaagggccg
    10621 cttcgaggtc gatgcggtgg cccaccgcat ccgctacgcc ggccactggc tggacatgac
    10681 ccgctacgag cacgcgctgc tgcacgaact gctgcgccgg ccgggggcga tcctcagccg
    10741 tgcgcaattg ctcgaccgcg cctgggagga cgcgctggaa agcggcgagc gcaccgtgga
    10801 tacccatgtg aagaccttgc gcgcgaagtt gcgcgcgctg gacccggcgc gcgacccgat
    10861 ccgcacccat cgcggcctgg gctattcgat cgatgtggac tgacggccac ccgcgcgcgc
    10921 catgaagatc ggcctgcgcg tcctgctggg gtacttcctg atcgtggcgg tagccgcgct
    10981 gctgctggtc aatgtcttcg tgcagcaggt caagccgggc gtgcgccagg cgatggaaga
    11041 caccctggcc gacaccgcca acgtgctggc ggaactggcc agcgacgacc tgctggccgg
    11101 gcgcatccac gagggacagt tcgccgggcg gatccgcgcg atgggccagc gcgagatcgg
    11161 cgccaggatc tggggccagc ccaagcacgc gcccagctac cggatcacgg tgaccgatgc
    11221 gcgcgggatc gtggtgtacg actccgccgg tcgcgacatg ggccgcgaca attcgcgctg
    11281 gaacgacgtc caccgcaccc tgcgcggaca gtacggcgcg cgttccaccc gcgacgatcc
    11341 ggacgacgag atgagttcgg tgatgtacgt cgccgcgccg atccgcgatg gccagggccg
    11401 catcgccggc gtgctcaccg tgtccaagcc gaaccgcagc atccagcctt tcatcgagcg
    11461 cagccaggac gtgatcctgc gctggggctg ggtcctgctg ggcagtgccc tgctggtcgg
    11521 cctgctggtg gcgtggtggc tgtcgcggca actggctgga ctgcgccgct atgcggatgc
    11581 cgtgtcggcg gggcagcgcg ccagcttgcc ggaacgcggc atcgagaagc ccgccggcga
    11641 gttcgccgac ctcggtcgcg cgctggagag catgcgccgg caactcgacg gcaagcagta
    11701 cgtggagcac tacgtgcaca gcctgacgca tgagttgaag agcccgctgg cggcgatccg
    11761 tgccgccgcc gagttgctgg agtcgccgtt gcccgatgcc gaccgtgcgc gcttcgccgc
    11821 cagcatcggc agccagagcc ggcgcatggc gacgatggtg gacaagctgc tggccctggc
    11881 cgaggtcgag catcggcagc gcatcgaacg gccggagtcg ctggacatcg ccgcactggc
    11941 gcgcgaagtg gcggacgatg ccgcatcgca tgggcaggtc gcgcaggtgc gcgtcgaggt
    12001 ggaggccggc gagggattgc cggacttgct gggcgatccc ttcctgctgc ggcggttgct
    12061 ggccaacctg gtcgataacg cgatcgattt ctcgccggtc ggcggcgtgg tcgaggtgcg
    12121 cctgcaggtc gcgggcgatg cgcttgcgat cgaggtcggc gatcgcggcc ccggcattcc
    12181 ggactacgcg ctggagcggg tcttcgagcg cttctattcg ctgccgcgac ccgatggcgg
    12241 catccgcagc agcgggctgg gcctcaactt cgtcgccgag atcgccaagc tgcacggcgg
    12301 ggaagcgtcg ctggcgaacc gtgatggcgg cggggcgatc gcccgcgtgg tgttgccgat
    12361 cggctgagac gcctcaccct cgcttcatcg tccgcccatc atggcgtcga atcccgacgt
    12421 gaccctgcac gcactttccg gcaaaggatg gagcggggcg atgcgattgg cattcaaggc
    12481 gatcatggtg gtggtgctga cgctggcgat cctggtgccg ctgacgatgg tgcgcggcac
    12541 catcaacgag cggcaggcat accgggagga agcggtggag gcggtggcgc agagttatgc
    12601 gcgtgcacag ggcatggccg ggccggtact ggtggtgccg tacagcgaag aagtggagtt
    12661 ggaggaaacc gacgcgaagg gcgtcctgcg caagcgccag accacggtcg agggccgctg
    12721 gctgtacttc ccgaagacgc tgacgctcga cggcaagctg ctgccctcga tccgcaagcg
    12781 tggcctgcac caggtccgtg tctacgaact gcagggcagg ctggatgcca cgttcgatgc
    12841 gcgcatcccc gaggccgacc cgatgcgtcc gcgcaagatc ggcacgccct ggctggacat
    12901 cggcatcgcc gacgtgcgcg gcctctcggg tacgccggtc ctgaagacgg gcgcgacctc
    12961 gctgccggtg ctgcagggcc agcagggccg cgacgaggcg ggcgtgcatg ccttgctgcc
    13021 cgacacggcg atcgtcgacg ggaagttggc gttcccgctg cagttccagt tcgcgttgcg
    13081 cggcaccgag gccctgcggg tcacgccgct ggcggacagc aaccgcatca cgctggcatc
    13141 ggcctggccg catccgcagt tcaacggtga tttcctgccg cgcgccccgc gagtggacgg
    13201 cagcgggttc cgcgccgagt gggacgtgtc ctcgctggcc agcaacgcgc aggcgcagta
    13261 ccgcgccggc aggtcggaga aatcggtgga gatggacggc atcgggctgt cgctggtgga
    13321 gccggtggac ctgtattcca agctcgaccg cgccagcaag tacggcctgc tgttcgtgct
    13381 gctgaccttc gtcggcttct tcatgttcga gacgatccgg cagttgccga tccacccgat
    13441 ccagtacctg ctggtcgggc tggcgctggc gatcttcttc ctgctgctgc tgggcctgtc
    13501 cgagcacatc gccttcggca tcgcgtactt cgtggccagc gtggcctgca tcgggttgct
    13561 cgggttctat cttgcgcatg tcctgcgcag ccgcgcgcgc ggtgtcggct tcgcggcgat
    13621 gctcggcctg ctgtacgcgg cgctgtacgg actgttgttg tcggaagaca acgcgttggt
    13681 gctgggcgcg ggcctgctgt tcgcgatcct ggccgcggtg atgatcgcca cccgcaaggt
    13741 cgactggtac caggtggcgg cgcgagcgcc ggggccggtg cgcatcgacc cgacgactga
    13801 ccacgttgcc tagaccacgt cgcctagacc acgccactag aatcacgaca tggacgacgc
    13861 gatcacattg gaatgcctgt ccggtgcggc gctgctgccg cacctggacg cggtggcggc
    13921 gctgcgcatc gcggtgttcc gcgactggcc ctacctgtac gacggcgatg ccgcctacga
    13981 acgcgagtac ctggccgcgt atgcgaagag tcccgacagc ctcgtcgtgc tggcgcgcgc
    14041 cggcgacacg gtggtcggcg ccagcaccgg catcccgctg gcgcaggaca gcgccgagtt
    14101 ccaggcgccg ttcctggcgc gcggcttcga cgtgtcgacg gtgttctatt gcggcgagtc
    14161 ggtgctgttg ccggcgtggc gcgggcgcgg catcggccat gccttcttcg acgcgcgcga
    14221 ggcgcatgcg cgcgcactgg gcggcttcgc gtggaccgca ttcgccgcgg tggatcgcgg
    14281 cgccgacgat ccgcggcgtc cacctcacca tcgtggcaac gacgcgttct ggggcaagcg
    14341 tggctacgtg cgccagccag gcatgaccat gcgcctgcac tggaacgaaa ccggcatcgg
    14401 cgacatcgac catcccctga cgttctggct gcggccactg gacgaggaca cacgcacatg
    14461 aaagtcgcgg catgcaagta cccgatcggc gaaccgcgcg atttcgccgc gttcgcggcc
    14521 aggcaggcag cgctgctggg cgaagcgaag gcacgcggcg cgcaactggc cgtgcttccg
    14581 gaatacctgt cgctggaact ggcggcgatg ttcgacgacg cgatccgtgg cgacctgcac
    14641 gcctcgctgg cggcgatcca gccgctgcgc gacgactggc tgtcgctgta cgcggggttg
    14701 gcgcgggagc tcggtatcgg catcgtcgcc ggcagtttcc tcctggacat ggggcgtggc
    14761 cgctatcgca atcgcagtga tttcttcgcc gccgatggcg ggcacctgtg gcaggacaag
    14821 ctgcgcctga ccgggttcga gaagcgcacg ggcgtgatcg actccggcga tgcgctgaag
    14881 gtgttcgatg cgggcggcgt acgcgcgggc atcgcggtct gctacgacag cgaattcccg
    14941 ctgccggtgc gcgcgcagtg cgaggccggc gcgcgcctgt tgctggtgcc cagttgcacg
    15001 gataccgacg ccggcgcgac ccgcgtgcgc gtgggctgcc tggcgcgggc gctggagaac
    15061 cgctgtttcg ttgcgcaagc cgtcaccgcg ggcgaggcgc cgtggagtcc ggcgctcgac
    15121 accaataccg gcgaggcggc gctgatcgcc ccgatggatg tgggcctgcc gagcgacggc
    15181 atgctggtgc agacccgcgg cagcgaggcc tgggcgatcg cagagctcga cttcgatgcg
    15241 ctggaagcca gccgcgcaca ggcgcaggtg gccaacgacc gcgactggtc ggcgcagtat
    15301 ttcccgaccg tgctgcgggc gcggctggaa cgtgccgggg atcacgaaac cgcggcaatg
    15361 ctggcccggg catgaccgat ggcccacgcc cggccacgcc gggcttacta gactccacgg
    15421 tttgtttgaa gaaccgggga tggcgatgaa gcgattgatg ttggcgacgg cgttgcttgt
    15481 ggccggcgtg gccggtgcgc aagagaaggt ggacctggac atgaccgggc gcatccgcgc
    15541 cgaggcgttc aatcgctcgc aggtgatggc caccctgaac cacctgaccg acgagatcgg
    15601 cccgcgcctg accaactcgc cgtcgatggc ggaagccaat cgatggacgg gctccaagtt
    15661 caccgagtgg ggcctgtcca acgtgcacga cgaggcggtc gacgacatcg gccgcggctg
    15721 gtcgttcaac gatgccggcg tggagatgct cagtccgcgc gtcatgccgc tgcacgcgct
    15781 gccgaaggcg tggacgccgg gcaccaccgg cccggtcgaa ggcgaggcga tcttcgccaa
    15841 gctgcagaac aagggcgacc tggagaagtg gaagggcaag ctcaagggca aggtgctgtt
    15901 caccgacgaa ctgcgcccgt acaagccgaa cgagaagtcc gacttcagcc gccatgacca
    15961 tgacagcctg gaagagcttc tgtcgtaccc gctgccgacc gagcgcagcc gtgatgcgtc
    16021 gatgctggcg cagttccgcg agcgcatgga attcggcccg ttgctcaacc agttcctgat
    16081 cgacgaaggc gtggtcgcca cgatgtcgat cagcagctgg aacgacggca tcgtccgggt
    16141 catgggcggt ggttcgcgca aggccggcga gtcggtcggc gtgccggcgc tggtgatgat
    16201 ggccgagcac tacaacacgg tgatgcgcgc gctggatcgc aaggagacgg tgaagctgcg
    16261 cgtcaacgtg gatgcgcgct tcctcgatga tgccaacaag ccgggctaca acaccatcgc
    16321 cgagatcccg ggcaccggcc cgaacaggaa cgaagtggtg atgctgggcg cgcacatgga
    16381 ctcctggcac accggttccg gcgcgaacga caacggcgcc ggcgtggcgg tgatgatgga
    16441 agcggtacgc atcctcaagg cggtgggcgc gcgtcccaac cgcaccatcc gcgtcgccct
    16501 gtggactggc gaggagcagg gcctgatcgg ctcgcaggcg tacgtggcca agcacttcgc
    16561 cgcctatccg gaacccaccg atccggcgca gaaggcactg ccggcggcgt tccgcaccaa
    16621 caagggcaag ctggtcaaga ccggcgacta cgagaagatc accgcgtact tcaacctgga
    16681 caacggcagc ggcaagatcc gcggcatcta tgcccaggag aacctggcga tcgcgccgat
    16741 cttcgaggaa tggctgaagc cctggaacga cgtcggcgcg accatcgtca cccagcgcaa
    16801 caccggcagc accgaccacg tcagcttcga ccgcgtcggc atccccggct tccagttcgt
    16861 gcaggaccag ctggactatt tcagccacgt ccaccacacc cacctggacg tgcaggacca
    16921 cgcggtggcc gacgacctca agcaggcctc ggcgatcgtt gcgtcgttcg tctacaacgc
    16981 cgcgcagcgt cccggcaagc tgccgcgcaa gatgatggtc gaggactgag tttcgctgtc
    17041 atcccggcga atgccgggat ccagcttctg caactgcgaa gggccggcat tgccggccct
    17101 tttattgtct ggctgcgggg cagtgccacc aagcttccct gttatctcac ccgaacaacg
    17161 acacccccgg caccagccac aacgacaggc ctgcgagggc gcaaccgatt cccgtcgccg
    17221 ccagcacgtc gctggggtaa tgcaggccca gcaccacccg cgaggccgcg acgctggcgg
    17281 tgaacggcac cagcagccag gccagcagtg ggtagtgcgc catcgccacc aggctgaagg
    17341 cgaccgcatg cagggtgtgc cccgagggaa agctgaattc gtccagcggg gccacccagg
    17401 cgcggatgcg cacgtcgctg gcgaagggac gcggacgctt ggtccagcgc ttgagcgact
    17461 tgtagaggat cagtgcgacc aggccggtga gggcgagatg cagcgaagcg cgcaggccgg
    17521 ccatcccgtc gagcacgatg agggcgccca tcagcccgta ccaggccacg ccatcaccga
    17581 gccggctgac cgcggcgaag taacgccgcg cggcttgcat ttcgcacacg gcattcgcgc
    17641 gcaggcacca gccggactcg gcatgggcga tgcgacgcgc ccgcgaccta gagggcagcg
    17701 atcgggacat gcccgtgtcc tcgttgggcg agcgattgca ggaggtgatc gaagtcggcg
    17761 gcgacctggg tcggccgcag cgcggacacc gcttcgcggc acgacggtcg catgcgccgt
    17821 cgtgtcgcct cgtcgctgcc gatgcgggcc accgcggcga tgaaggcggc atcgtcgcgg
    17881 gccggctggc ctgtgcgccg gatcgcggca ccgtgcaggc catcgcgcag gcattcgcgg
    17941 gcggcgccgt agtcgaaggc cacggtgggc acgccgctgg caagcgcttc aagcgtcacg
    18001 ttgccgaacg tctcgctatg gctggggaat acgaacaggt cggcgctggc gaaatggcgg
    18061 gcgagcgctt cgccgcgttg caggccgcag aaaatgaagt cggggttgtc gcgttgcagg
    18121 gcgtcgcgcg cggggccgtc gccgacccag ataaagcgcg cgtccgggcc ctgccgttgc
    18181 agggcgcgga acgcctccac cgccagcgcc aggttcttct ccgcggcgat gcggccgacg
    18241 aagatcgccg caaccccgcg ctcacccacc ccccacatcg ctcggagcac cgggtcgcga
    18301 tgggcgggat cgaagtgccc tgtatcgacc gcacgcgcta gccgcaccac gttgtcgaag
    18361 cgttcggctt ccagcagcgc ctgcagttcg cgggtcggga ccagggtggc ctgcgcgctg
    18421 ttgtggaagt ggcgcatcca gcgcagcgcg accggcgcca gccacggcat cccgtagtcg
    18481 cgcatgtagg cgtcgaaacg cgtgtggaaa ccggtggcgg cggggatacc gagctggcgc
    18541 gcggcacgca gcgcggacca gcccagtggg ccttcggtgg ccacgtagag ggcgtcgggt
    18601 cgttgcgctt gccagaggcg gcgcagtgtc cgcgtggccg gcaggccgaa ccgaaggccg
    18661 ggatagcggg gcagtccggc gccgcgaacc agcagggtgt ggtcggaaat ggtcgtcgcg
    18721 gtatcgccgg cctggcgcgg gcgcaccagt tgcacggcgt ggccgcgctc gcgcaggccg
    18781 tgttgcaggc cctggacggt cagggccacg ccgttgatct ccggcggata ggtctcggtg
    18841 acgatcgcgt agcgcatgcc gctctccgtt tgcgggaggc tgcgcggcgg gggtgaccgg
    18901 gtggtgtcgt tgcgatgacg agggggtgac agggccagga aaagtggggt cagagtcggc
    18961 tttcgcgtaa cgaaagccga ctctgacccc acttttcccc ggtttcgtcg caacccggct
    19021 tgacgatccg ggtctgttcg tactgtacta gatcaactag tacagatagg acggcgccga
    19081 tgggacgacc ccagcccctg atgatccaga tcgccaccgg cgatccgcgc ccgatcggca
    19141 agcagatcgt ggacgccgtg cggatgaaga tcgccaccgg cgaactgggc gccggcgacc
    19201 agctgcccag cgtgcgcggt ctggcccagc agctgaccat caatccgaac acggtggcca
    19261 aggcctacgc cgagctgacc accgagggtt ggctggaatc gcggcagggc atgggcctgt
    19321 acgtggccac cccgcgccag cgcctgtccg aggacgaacg cgagcgccgc ctggacgatg
    19381 ccatcggccg cttcgtcaat gacgtgatcc cgctgggcta tgcgcccgac gaggtgcagg
    19441 cgcgcgtggc ccacgaattc cagcaactgg ttccccggaa gatcgcctga gcgcgattga
    19501 cagacatcga gcacacacat gacagaccaa cacgactacg tcatcgagac ccgcgcgctg
    19561 agcaagcgtt acggccgcaa gctggcgctg gacaacctcg acctgcgcat cccgcgtggg
    19621 cgcatccacg ccatcgtcgg cgccaacggc gccggcaagt ccaccctgtt ccgcatcctg
    19681 atgggcttca tgccgccgac caccggcgag gcccgcatcc tcggccgcga cagccagtcg
    19741 ctcacgccgc aggaccgcgc gcgcatcggc ttcgtcaacg aggaacacac cctgcccaac
    19801 tggatgcgag tcgagcaggt gacgacgatg cagaagcacc agtacgcgcg ctggaaccag
    19861 caggcattcg acagcgtgat cggccactac cacgtgctgc ccgagcagaa ggtcggccag
    19921 ttgtcgcgcg gcgaacgggc cggtttcaac ctggcgctgg ccttggcgca gcgaccggaa
    19981 ttgctggtgc tggacgaacc cacgctgggc ctggacgtgg tcgccaagcg cgcgttcctg
    20041 gagtcgctgc tgtacagcaa cgccgccgac gactgcacgg tgatctattg ctcgcaccag
    20101 atggaggaga tcgagcgcgt cgccgataac ctgatcatcc tggagcgcgg ccagctgaaa
    20161 cacatgtccg cgccggagga cttcaccgcg cgggtcagtc actgggtggc ggacgtgccg
    20221 ttcaaggggc ccgagctgca taccgtgccg ggcctgctgg aagtgcagcg cctcgacggc
    20281 ttgcaccact acctcgtgct ggaccaggac gacggcttcg aacacttcct gcgcgccagc
    20341 ggtgcgcgca acgtgcaatc gatgccggtc ggcctggacc gcgccgtcaa cgccttcctg
    20401 accaagaacc acgctgcgcc ggctgcggcc tgacgcgcga cagaggatac gaacatgatc
    20461 gatctcttga aaggcgagct cctgcgcttc cgcctgtggg ccggcatcgc cgccttcctc
    20521 aacatcgccg tgctgggctt cctcagccgc atggtcgacc tcgcccagca accgctcgtg
    20581 gtctaccagg tgttcgccgc ggtttacgcg gtggccggca tcctgctggg cctgtaccag
    20641 atgggcagct accgcaagcc gaaccagtgg ctgaacctgt tgcatcgccc gctccatcgc
    20701 ctgcgcatcg ccggtgcgct gaccggcgcg ggtgcgctgc tgttgctgct ggcggtcgcg
    20761 gtgccgatcg cgctggtcgc gttgtaccag gacacgatga ccgcgcgcgt ggtggacctg
    20821 cgccactgga tgctgccggt ggcggcgtgg ctgatcgcgg tgtcgggcta cctggccggc
    20881 gcctatgcga tggtcggcaa ccgccgctat tcgttcgccg cgttcctgct gcccaacctg
    20941 ttcatgtacg cgcaggccac gggcgtggcg atgctgggcg tgcagtgcgt ggtcatcgcc
    21001 ttcctcgcgg cgatgctggc gatcgcgttc aagccggatc ctgccgcgcc gtcgcacaat
    21061 ccggccgcgg tgctggcgat cgcgttgccg gtgcagctcg gcgcgtattt cctggtgtgg
    21121 atgctgggct acggcgtcga aatggcgtgg acggtcaccg gcacgcaccc gctcaacatg
    21181 cccgcgccgc cgaagggcgg ttacatcgag gccgatcgcg cggaaggcaa ggacatgctg
    21241 ctgctcggca tcgaaggcag ccgcgatccc gaggccgcgc tgtggcgcga gcagatcgcg
    21301 ctgtcggacg tgtacacgac ctatccactg cgcaacctgc cggtccgcaa cagcctgacc
    21361 aacctgtcgc cgctggagtt cgccgatggc gacaacaatc tcttcctggt ctacagccac
    21421 gatcgcgcgc gcttcgtcac ccgtggcctg cgcgaccagc gccggatcgg cgagatgggc
    21481 gtcggcgaag gacagtccgc gttccccgca ccgacgatgc cgtttggcgc cgagtacctg
    21541 tacaacgcga acgcggccta ccagtacgac ggcgaacagc agcgcatctt cgagcgcatc
    21601 cgcctgccgc ggggcgaggt gatggccagc ccgcccgcgc cggccggcga caacctgctg
    21661 gtgctcagcg accgcgccgc ctatttctat cccggccgcg aggcgatgaa cgggctcgac
    21721 ctgctgcaac cgctgatgcg ggtggcgatg ccggggcagg tcggcaacct gagccggatg
    21781 gacgtggtcg aactgctgga cggctacctg gtgtcctaca cctacacgtg gggcgtgtgg
    21841 agcggcgaac tgcagcagcc gttccagcag gtgctgcgcg tggacggcga gggccaggca
    21901 cgggaggtcg gacgccgtgc gctgtcgatc gacctgccgt ccgtctacac caaccgcaat
    21961 acctggttgt cgccggtgct gcgtgcgatc tgcctgggcg cgcaggacct gttcgccgcg
    22021 aaggacccgt tgcgcgcgaa gcccgagccg atgccggtgc agatcctgtg gttggccggt
    22081 gtgtgctgcc tgctgtcgct gctgggcgcg gtgtggctga gcggcaggca ggcgcattcg
    22141 ccgcgcggtc gatgggcgtg ggtgttgctg tgcggcgtgg tcggcctgcc tgcgctggtg
    22201 agcctgtggt tgctggttcc gcgtcgcgaa gccctgccgg tggccgcgcc agatgcgcag
    22261 ccggtcgcgg cttgatccgg aacgaacgag gacatcgaca atgactagtc cgttccgtca
    22321 cttctgcatc gtgctggcat tgctcgcggc gccgttcgtg ccaaagcccg cgtcggccgc
    22381 gccaggcgaa ggcgacctgc gcagcgccat cgccgcacaa cgcagcctgc cgcgcgccgc
    22441 gcgcctgcca cgcacggctt tcctcgagaa tcgcgccctg gtcggcgtac agcttgcgcc
    22501 ggccggcgac gcggtggcct acctgcgcga gcagaaggac tcgcgcgcgc tgtggctgct
    22561 gccggtcgat ggcggcaagc cgcgcatgct ggtcggcagc acccaggccg accagctcct
    22621 gtggtcgcgc gacgggcgct ggctgttcct gcgcgcgtcg cgcgtgctga ccatcgtcgg
    22681 tatcgacggc cgcaccggcg tgcgggtgcc gctgggcggc accgaacggc ggcgcttgat
    22741 gaaggtggat ccgtcgcagc ctgccgccgt gctgctgggc gaacgcgtgc gcgtcggcgc
    22801 cagcgaacgc tggcgcgtgg tgcgcatgga cgcgcgcggc aagcgcaccc tgctgcgcga
    22861 ggacgcgcgc tggatccacg atgtcgcgct ggacgcgcgg ggccgcatcg tcgcgctggc
    22921 gcgtttcgaa ggcgaccacg atgcgctgta tcgcgtgcgc gcggacggga ccctgcgcga
    22981 cgtgctgcgc ctgcaggcga tggagcgcct ggacctgctg gcggcgccgg ccgacggcag
    23041 cctgctgatg accgggaatc ccggcagtgc ggctgcaggc ggggatttcc gccgggtgat
    23101 gcggctggac gcgaacgaca ggctgcagac cttgcaccag gatccccgcg gcgaagccga
    23161 tctggacgac gtcgtggtcg acccgcgcac gcagcagccg ctggcggcga gttaccgcag
    23221 caccgtcgcc gcgacgtacg gcatcggcgg cgcgcagggc gtcgtcgcgg acatcgcgac
    23281 gcgattcccc ggccgcgata tcggcctgca ggcaggcagc ggcccgtccg cgcgctggct
    23341 ggtgagcgaa cgcgcggcga ccctgcgcga cccgcgctgg tggttgtacg acccacgcaa
    23401 cagagagctg cgccgcatcc tcgacgaccc cggcatcacc gccggcacgc cggacgaaac
    23461 cgcgctggcg cgcaaggtcg cgatcgacta cgtcgcctcc gatggcaggc gcgtgcacgg
    23521 gttcgtcctg ttgccgcccg gtttggatgc cgcgcgcgcg ccgctggtcg cgcaggtgca
    23581 tggcggcccg atcagccatt tccgcacggg ctacgacggc gttgcccagt tcctggccaa
    23641 tcgcggctac gtggtgttcc agtcgaactt ccgcggctcc accggccacg gccgcgccta
    23701 cacgttcgcg tcgaacggcg actacggcaa cggccgcgtg caacaggaca tcgtcgaggg
    23761 cgtgcgctgg ctgctggggc agggcatcgg cgatgcacgg cgcgtcggca tcgtcggcca
    23821 ttcgttcggc ggctattcca ccctgcaagg cctgacgttc cagccggagc tgttcaaggt
    23881 cggcgtggct ggcgcgccac cgtcggacct gggctggggc atgcgctggc tggtgcagtc
    23941 cggcgaccag ggcgacctgc ctgatcgttc gctcaagcac acgctggcgg cgttgtcgct
    24001 ggatgcgacg gatcccgcca cctatgcacg cctgcatgcg caatcgccgc aggcgaacgc
    24061 gatgaagatg cgacgcccgt tgctgatcat ggccggcggc gccgaccgca cggtggcgat
    24121 ccgcgacgtc atccattaca gcgccacgct gaagtcgctg gggcgcgcgg tcacgctgct
    24181 ggtggagccg ggcggcggtc attccccggt ggacccggtg ccgcgcgagg cctatctgtt
    24241 cgcgatggaa accatgctgc agcggcacct gggcggtccg gtgccggaag cgccgggcca
    24301 gcgcctgcgc gcctacctgc gcgagaacct gcggctggca gggcccgaat tcgcggggtt
    24361 cgcgaagaag tagaaaagtg gggtcagagt cgactttccc cgcaagatat tgaaacgttg
    24421 agaggggtcg ctgcgaaagt cgactctgac cccacttttc acttcaatcg ctcgagccgg
    24481 cccttgcgct tgaccaggct cctgtaatcc cactggatgt cgcgggcctt gcccagccaa
    24541 tgctggagca aggccgcgtc gacgtcgccg gcggtcgtgt agcgcgcttc ggcggccttg
    24601 aacgtgccct cggcccgcag tcccggttcg tcgaaacttt gcccactcca gaacagcagc
    24661 ctcaccgact ccttgagctt gctgtagccg accaccgggt taccgtccag gaaccagacc
    24721 gggtgcgcat gccagatcct ggcctccgcc tcgggcaggc cgcggtggat cgcttcggcc
    24781 agcagatcgc agatcgcgcg gtcggcttgc gattgccgcg cgttgtagtc gtggatgtcc
    24841 ggggtcatgc gctcactccg cggcgagctt gagggtcacg cccagcccga gcatgtcgtc
    24901 gggttcgccg gccaggtcgg cgcgcagcag cgggcgggca cgcagccagc gttcggccag
    24961 cgccagttgc aggcgctcgc catcggcctg caggcgcagc acggggagtt cgtcggtttc
    25021 gtgcgcgcga tgcagcagca cggccagccg cagcagggcg gcgacgtggc gggtgttggc
    25081 cagcaagcgg tcgggaatca tgtcgaaggc cgacttcggg atgccgcggc gatgggtgcg
    25141 cacgagtgcc gccagcacgc gctgttcctg ctgcgagaag ccggcgatgt ccgagtgctc
    25201 caggatgtag gcaccgtggc cgtggtagcc gctgtgggcg atggccaggc cgatctcgtg
    25261 caggcgcgcg gcgcgcgaga gcatgcgccg gtcgtcgcca tccagcgtcc acgcgggcgc
    25321 gacctggtcg aacaggcgca gcgcggtggc ttcgacccgc gcggcctgcg cggcatcgat
    25381 gccatagcga tgcatcaggg cctccaccga gacctcgcgc ggatcgtcgg cgccgccgcg
    25441 gcccagcagg tcgtacagga cgccctcgcg caacgcgccc ttgctgacct gcatgcgctg
    25501 caggcccagc gcggcgaaca ccgcctcaag caccagcacg ccaccggcga tgatcgggcg
    25561 tcggtcgccc gacaggccgg gcaggtcgat gtcctcgatg cggtcggcct gcagcaggcg
    25621 ctcgcgcacc agcggcagcg cctcggccgt gatcgcaccc ttggtcagct tgagtgcggc
    25681 gcagatctcg ccgatcgcct tgttggtgcc cgacgaaccc accgcctcgt cccagcccag
    25741 gctgcggtat gtcgaggcga actgctggaa ctcggcggtg atctcggtca gcgccgagtt
    25801 ccatttcttc ttcgacaggc gtccgttggg gaagaaccgg cgcgtgctgg cgatgcagcc
    25861 ggcctgcagg ctttcgcgct ccagcggctg catgccgcgg ccgatgatgc attcggtgga
    25921 accgccgccg atgtcgatga ccagccgcag ttggtcgtcc ttgggcggct gcgcgtgggc
    25981 cacgccgaga tagaccaagc gcgcttcctc gcggccggag accacttcga tcgcatgccc
    26041 cagcgcggcc tccgccggga ccaggaagcc ggcgggcgca cgcaactggc ggacggtgtt
    26101 cgtggccacc gcgcgcacat gggccggcgg gatgtcggcg atgcgctggc cgaagcgcga
    26161 caggcagtcg atggcgcgcg cctgcgcctc cgcggacatg ccgccgaacc cgtccaggcc
    26221 ctcggccatg cgcacggttt cgcgcagccg gtcgaccacc cgcagctggc ccaatacccg
    26281 ttgcgccacc accatgtgga aactgttcga gcccaggtcc agcgcggcca gcatgtcgcc
    26341 gtcgcgcggc gcggacgcgc ggttggccgg ggtgcggcgt tgggtcgtcc tcatgcgatg
    26401 ggcaggccgc gttgcgtcat ccgcgcatta tcaccgcatc gacctgcgcg gcatcacgcg
    26461 tacaggccgt ccagcagcga caactgcgcg gagaacggct cctcgcccgc agctggaatg
    26521 cacttggcat agctgccgtc gggctggacc tcccacgcgt tctggttgtc gagcaggtag
    26581 ttgagcaggc cttcctggtg tacgcgccgg gcgcattcgg cgtcgaggat ggggaacgcg
    26641 gtttccaccc gccgcagcag gttgcgctcc agccagtcgg cggaggagca atacagctcc
    26701 ggctggccgt cgttgccgaa ccaccagatc cgatggtgtt cgaggaagcg accgaccacc
    26761 gagcgcaccc ggatattctc cgaaatgcct tcgatgccga cccgcaaggt gcaggcgccg
    26821 cgcacgatca ggtcgacctt cacgccggcc tgcgaggcct cgtacaaggc gagcatcacc
    26881 tgcggttcgt tgagcgcgtt catcttcgcc acgatccggc cggggcggcc cgcgcgagcg
    26941 tgcgcggtct cgcgggcgat gcgctcgagc acgcccttgt gcagggtgaa cggcgattgc
    27001 agcaggtgct tcagcgtcag cggcgcggcc aggccggaca gttgctggaa gatcaggtgg
    27061 acgtcgttgc cgatgtcggg gtcggcggtc atcaggctga agtcggtgta cgcacgcgcg
    27121 gtgccgctgt ggtagttgcc ggtgcccagg tgcacgtagc ggcgcaggga tttcccctcg
    27181 cggcgcacga tcagcagcat cttggcgtgg gtcttgtagc cgaccacgcc gtacaccacc
    27241 tgcaccccgg cgtcctgcag gcggtcggcc aggcccaggt tcgcttcttc gtcgaagcgt
    27301 gcgcgcaact cgaccaccac ggtcacgtcc ttgcccttgc gcgcggcctg gatcagggag
    27361 tcgacgatcg ccgactcctt gcccgtgcga tagagcgtct gcttgatcgc cagcacgttc
    27421 ggatcctcgg cggcctggcg gatcagctcc agcaccgggg tgaaggcatc gaacggatgg
    27481 tgcagcagta cgtcaccctg ctggatcagg tcgaacatcg catcgacctc gcgcggcatg
    27541 cgcggctgga acgcggggaa cttcaggtcc gggcgctgca ccaggtcgta cacctggacc
    27601 acccggttga ggttgaccgg gccgttgatc ttgtagaccg cgttggcggg caggtcgaag
    27661 ttggcgagca ggctgtcgac gatgtcctgc gggcagcgtt cgtcgatctc cagccgcacc
    27721 gcgcgcaggt agccgcggcc cagcagttcg tcgcgcaggg ccagggcgag gttgtcgaca
    27781 tcgtcctcgt cgacgatcag ctcggagttg cgggtgaccc ggaactggtg cgcgccgttg
    27841 accttcatcc ccgggaacaa ctcatgcacg aacgcggaca gcaccgagct gaggaacacg
    27901 aagtcgtgga actggtcgcc ttcgccgtcc tggtcgggca ggcggatgat ccgcggcagc
    27961 gagcgcggcg c
//
LOCUS       DAOOCX010000015        27550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1391_length_27550_cov_7.458447, whole genome shotgun sequence.
ACCESSION   DAOOCX010000015 DAOOCX010000000
VERSION     DAOOCX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 27550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27550
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1391_length_27550_cov_7.458447"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="PK399_02610"
     CDS             complement(<1..117)
                     /locus_tag="PK399_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease IX"
                     /protein_id="HPW12052.1"
                     /translation="MTQPTLHLVDASMYVFRAWHSMPDEFHDADGWPTNAVHG"
     gene            complement(124..711)
                     /locus_tag="PK399_02615"
     CDS             complement(124..711)
                     /locus_tag="PK399_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HPW12053.1"
                     /translation="MTTSPTTLTLAALDSRLSVPTRQLEAPGPDHDTLLRILRSAVRV
                     PDHGKRVPFRFLRIAGDDRKALADATIARLLQRESRPAEGAIEKLRNRFTLPPLTIAV
                     IARLGDDEKIPASERFSTASCVCLLLLQAAQAEGYGAQWLTGWPCYDRPFLENTLGLQ
                     ADEQLVGTIAMGTPQVDVPDRERPDPRDLLTDLTL"
     gene            789..1880
                     /locus_tag="PK399_02620"
     CDS             789..1880
                     /locus_tag="PK399_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HPW12054.1"
                     /translation="MAVNIGVARESVAGERRVALTPETCRKLVAAGATVRIESGIGDG
                     AYFPDQAYVDAGAHVTAHPREGADIVLCVQPPSAAAIGSLKSGAVLVGSLQPQADPAR
                     GEAITSRGIVAFPLEKLPRTTRAQAMDVLSSQAGMAGYKAVLIAAQLAPRFFPMLTTA
                     AGTIRPSKVLVVGAGVAGLQAIATAKRLGAAVEGFDVRPETREQIESLGGKFLDLGVS
                     AAGEGGYARALTDEERALQQQRLADHLKGIDVVVCTAAVPGRPAPKIISAAMVAGMKP
                     GSVVVDLAAETGGNCELTQPGETIEKDGVTIAGPLNLASMGAQHASEMYARNVYNFVS
                     LLLKDGALAFDWDDELLAKTVWPERVVAA"
     gene            1870..2646
                     /locus_tag="PK399_02625"
     CDS             1870..2646
                     /locus_tag="PK399_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW12055.1"
                     /translation="MLRKSLAIAAAAILAIYLGICGLLYGSQRAYLYFPVPRRAEVPS
                     FVMTSGDADVVVSTNGVDSPQAVVYLGGNAEDVSQAVALLATAFQGRAIYAMHYRGYG
                     GSTGSPSEHALVADAGRLFDQVATNHQGIVVVGRSLGSGIALQVAATRRAERLVLVTP
                     YASIAELAAAQFPLFPVGLLLQDRYESWRYAPRIKVPTTVIVADRDEVIPNASSRRLA
                     ECFVPGIATVVNFPDAGHNDVSDAPGYPAALAGSGPATTE"
     gene            complement(2651..3082)
                     /locus_tag="PK399_02630"
     CDS             complement(2651..3082)
                     /locus_tag="PK399_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HPW12056.1"
                     /translation="MNRTIRLTILALALWPAFAFAQSAAVPNQYPEWDQLTQAQRDAL
                     VAPLRERWNRNPDERARMAERAQRWQSMPRDQRERAGHGMQRWEHMSPEQRGEARALF
                     HAMRGLDKEQRKAFMAEWRQKTPQQKADWLKAHPAPEKRER"
     gene            complement(3090..3458)
                     /locus_tag="PK399_02635"
     CDS             complement(3090..3458)
                     /locus_tag="PK399_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12057.1"
                     /translation="MNTHSDHTGSDARFDEAVRRTHAVAVDRLSPRVRAQLAQRRNAA
                     LRGEAVAPARVGLRYAAAGFAALCALAIGLQFGSMPAPASLEPGIASTSATPDTTMLD
                     EDPEFYAWLASADAQQIAME"
     gene            complement(3455..3991)
                     /locus_tag="PK399_02640"
     CDS             complement(3455..3991)
                     /locus_tag="PK399_02640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPW12058.1"
                     /translation="MAVAAPAPLPTSLEQFLAGIDGRAFRFAELGLRHREDALDAVQD
                     SMMKMLGYRDRPPAEWSPLFWSILRNRIIDIQRRGLFRLRWLMPGSTDKDGEPVDWAD
                     DGPDPSRHHDSREAWSRISEALRTLPTRQREAFTLRVLEELDVADTARAMGCSEGSVK
                     THLSRAREALQRQLEEFR"
     gene            4212..4511
                     /locus_tag="PK399_02645"
     CDS             4212..4511
                     /locus_tag="PK399_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HPW12059.1"
                     /translation="MGDGFVALYIFMLAAIAGDVIISRVPVILHTPLMSGSNFIHGIV
                     LIGAMVVLGHADTPVEQALGFVAVLLGAGNAAGGYVVTERMLEMFKPSQKTEPKA"
     gene            4508..5896
                     /locus_tag="PK399_02650"
     CDS             4508..5896
                     /locus_tag="PK399_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HPW12060.1"
                     /translation="MSLLVLVKLSYFVAATLFLLGLQRMASPKTARSGIQWAGAGMLI
                     ATFATFFLPGLHNIGLMVAAIVIGVAANWAWGKKVAITDMPQMVALFNGMGGGSAAAI
                     GAVELVRWSAAGQSPGMVPGVLAVLGSLIGAVSLTGSIIAWAKLDGRMDKRYTFPGQQ
                     WFNAVIALAAVVCGAMALQTLAMPWIVAFFVLALALGVLMTLPIGGADMPVVISLYNA
                     FTGLAVAFEGYVLGNEALIIAGTMVGAAGMLLTRLMAKAMNRKISNVLFSDFGGGSGE
                     MQEIGGSMKPIEAADVAAMMAYAERVVIVPGYGMAVAQAQHKIWELSQRLMERGVKVK
                     FAIHPVAGRMPGHMNVLLAEAGVPYDLIADMDDINPEFANTDVSLVIGANDVVNPVAK
                     TDPASPIYGMPILDVVNSKNTIVIKRGKGTGFAGIENALFYADNTRMLYGDGAGVASA
                     LVSELKALDGGH"
     gene            complement(5862..6305)
                     /locus_tag="PK399_02655"
     CDS             complement(5862..6305)
                     /locus_tag="PK399_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12061.1"
                     /translation="MTTDPTTTPRWFALPLLALGMLGFVCAWVLAALALERQCAWLAP
                     LAALDMVLLLGLSRWPAGATRAGWAFAATAMAIVLANFGIAAGELGQGFGLRPWESAL
                     MLGANHAWTLLEMANSPLDLAFYASGLLVALVAGLNARRRGPSVR"
     gene            6393..6944
                     /gene="sufT"
                     /locus_tag="PK399_02660"
     CDS             6393..6944
                     /gene="sufT"
                     /locus_tag="PK399_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HPW12062.1"
                     /translation="MYSRSSEPVRFERDCAAVLVPQGDEVTLPAGTVGYITQSLGGSW
                     TVFVEGNLMRIAGKDGDAIGKEPPAPIALPEGASDEDVEALVWQQLRTCFDPEIPVNI
                     VELGLVYSAEVRPDDEGGRVVEVRMTLTAPGCGMGEVLVDDVRSKLEMIPTVAEADVE
                     LVFDPPWGRSMMSELAKLETGML"
     gene            7373..9646
                     /locus_tag="PK399_02665"
     CDS             7373..9646
                     /locus_tag="PK399_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HPW12063.1"
                     /translation="MNRFSRINMLATAIALSLVATGAQAAGKVDLHKRDIAQLKQQYQ
                     SQVAARGVAKMAHARHAQFMRADGDSTLLMRAQRADYGVRNYRYDQTFRGIPIFGEGV
                     VVSEDGAGNVRTMFGNLYSGLDQDIASTTPRLSKAAALVAGKRAGLGDRISGMLVENE
                     SADLRVYIDESGRGHLAYVVSFFADAIGAGSPTRPLIIIDANTGRILKQRENLQHALV
                     GTGPGGNAKTGQYEYGTNYGYLDVTQSGTTCTMNSTNVKTVNLNNASTNTSNTAFSYT
                     CPRNTVKTINGAYSPLNDAHYFGQVVFNMYQAYIGQAPLSFQLVMKVHYKTNYENAFW
                     NGTAMTFGDGASTFYPLVSLDVSSHEVSHGFTEQQSGLCYDNGSQCGGMNEAFSDMAG
                     EAAEYYSRGTNDWKVGAEIFKGTGALRYMNNPTQDGSSIDNAANMTSSMDPHYSSGVY
                     NKAFYNLATKAGWNTQKAFQVFARANRDYWTANISWNNGACGVQTAATDLGYTVADVT
                     SAFSAVGVSCSGGGGGGGGGSTGGVLTKGVAVTGINQTAGNAVNYTMVVPAGSTNVTF
                     TMSGGTGDADMYVKLGSAPTDSSYDCRPYKSGNAESCTFASSTGGTYYIRLKAYSAFS
                     GVSLVGDYTAGGGGGGGSTQTYTNTTDYNIPDNNATGITSTINVSGRTGNAPSNTQVA
                     VNIVHTYSGDLVVELVAPDGTVYTLQSRVGGSADNIVKTFTTNLSSEAINGAWKLRVK
                     DRASTDTGYINSWSITM"
     gene            9770..11767
                     /locus_tag="PK399_02670"
     CDS             9770..11767
                     /locus_tag="PK399_02670"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPW12064.1"
                     /translation="MIRKRLLQGLLLASALAGGAAFAGEGATAHARAHASAELASMHL
                     EANPDATIYIVTSNNTYQRVRGMTRNGVARSDSLGQPLVLAEIRAGQLSALSEDIHTM
                     EKRCGGYFAFATRAAAEAFLRADRAAAAVGRSLAASYTLDNQATVGPWLSQVAAANIQ
                     QTITQLQGYQNRYYSSPTGKTSAEWIRTSWQALANGRTDVTTELFACSTCSTQPSVIL
                     TIQGTDLPNEVVVLGAHLDSISGSGGNTVVAPGADDDASGIATLTETLRIALANGWKP
                     KRTVKFMGYAAEEVGLRGSNAIATSFRNAGTNVVGVLQMDMTNYQSGSATAMRLITDY
                     SNADLKTFFNQLFDAYLLPLGYTRGTYTCGYGCSDHASWTSAGYPAAMMFEAGTAGGG
                     MNPNIHTTADTLANMGNTAQPSVKFAQFSLAFLGELAKTQGTGGGSGGTGGALANGVA
                     KAISGTTGSSQVWTLAVPAGATSLKFTTAGGSGDADMYVKFGSVPTTTSYDCKADASG
                     NAGTCSIATAQAGPYQVLIKGYSAFSGATLTGSYATGGGGGSTQTYSNGTDVTITDNN
                     ATGVTSTITVSGRTGNAPSNTQVAVNIVHTYSGDLVVDLVAPDGTVYNLQNRSGGSAD
                     NIVKTFTTNLSSETINGAWKLRVADRAATDTGYINSWSITM"
     gene            complement(11874..12362)
                     /locus_tag="PK399_02675"
     CDS             complement(11874..12362)
                     /locus_tag="PK399_02675"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HPW12065.1"
                     /translation="MDNLLIVTTGGTIDKVYFDAKSDYQVGDPQIGRMLQELGVAFRF
                     HVIPLLRKDSLFVDDSDRQLLRATIAAQDEACVLVTHGTDSMVETARVLAGIEGKTIV
                     LTGALSPARFEGSDAEFNIGCAVGAVQSLPPGTYIAMNGRIWDPLKVRKNVDANRFEA
                     LG"
     gene            complement(12403..>13546)
                     /locus_tag="PK399_02680"
     CDS             complement(12403..>13546)
                     /locus_tag="PK399_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPW12066.1"
                     /translation="AADMLPAKILLGDALVHAGDFDEAASQYRDALRLHPACGDAWRG
                     LSNIKTRALDDEDARALAAQLQRRDIADSDRVAMGHALGKLHEDQGRHADAHAAFVAA
                     NDLQKRLTPWSRQAFEQFVARALAASEVLPSPLDPELGREAIFIVGQPRSGSTLFEQV
                     LAAHPEVEGASELPDLGIVIQQESMRRGVAYPQWVPQATADDWHRLGRAYLDRTARWR
                     ADKPRFTDKLPENWKHAGILRAMLPGATVIETRRDPLETAWSCFKQQFYSQPHFANDP
                     GDIAAHIRGSEGAMDAFRARDPARIRLHRYEDLLADPEPRIRALLDDCGLRFDSACLA
                     SHETRRPVRTASAAQVRQPLRRDTARASAYGPLLDPLRAALGRDRR"
     assembly_gap    13547..13646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13647..14135)
                     /locus_tag="PK399_02685"
     CDS             complement(<13647..14135)
                     /locus_tag="PK399_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW12067.1"
                     /translation="MSADPRMTGLPRAVVELVHAGARALRDGQPPRAEQLLMQACMQA
                     ATHPEPLRYLAILQLHTRRAALATQTLQRALALAPGDALLHCDLGTAQSASGDIDAAL
                     ASWRRACELDPAQPMPWFNLGRNLQQLGRTDEAIAALRQVNAIAADMLPAKILLGDAL
                     VHA"
     gene            complement(14132..14470)
                     /locus_tag="PK399_02690"
     CDS             complement(14132..14470)
                     /locus_tag="PK399_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HPW12068.1"
                     /translation="MKALRPLAFALLALTLFYAAWFAGDEHLLTGLLVFALPPFLLAI
                     AAWRGWHRAGFAAGVLALLWFSHGVMLAWSEPAQRGMALLEVALALAVIYASCLPGMQ
                     ARREKRRNAG"
     gene            complement(14467..15060)
                     /gene="wrbA"
                     /locus_tag="PK399_02695"
     CDS             complement(14467..15060)
                     /gene="wrbA"
                     /locus_tag="PK399_02695"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010367335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HPW12069.1"
                     /translation="MPEILVLYYSRGGSVARLARQIARGIGEVDGMQARLRTVPPVAP
                     TTTIASPPEPEDGAPYVDKRDLAECAGLLLGSPTRFGNMAAPMKHFIDTLGAEWASGT
                     LVGKPAAAFTSTATMHGGQESTLLTMLVPLLHHGCVVCGIPFTESALNTTRSGGTPYG
                     ASHVAGGQDDPELSDDEAALARALGRRVADLARRLQA"
     gene            15200..16471
                     /locus_tag="PK399_02700"
     CDS             15200..16471
                     /locus_tag="PK399_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HPW12070.1"
                     /translation="MEPLDSLNRWNDRLRDRARAGSFGRFVLKRFLGDRLFEAAGALS
                     YTTAFALVPLSMVVFGVLSAFPVFDHWSQSLKAYIFSNFVPSAASAVSGYLTDFSANT
                     KSLTAAGALALIVSLLVTLTSVEAVFNRIWRVPTARPKFGRFLVYWTVLTLGALVAAT
                     SLALLTRFFALAIFETVPGRWLEGLMLRVMPMAIELLAFAAIFKVVPHRTVKWRHAFA
                     GAALSVFLFECVKSGLALYLGSFNSYQTIYGTLAIVPILMLWIFLGWTSILFGASFAS
                     SMSAFRYQPLALRLPHGYELYGLLRMLGRFQQARSAGKGLHSDEIQQLEPILTDSLVQ
                     ELLGQMAAIKVVSRAEDGQWLLARDLDDVSLGELYEAAHLRVPVAEARLPHHDDALGV
                     AVMEVINGLRMPLRDLLKQRVSCVYERIPGE"
     gene            16497..17045
                     /locus_tag="PK399_02705"
     CDS             16497..17045
                     /locus_tag="PK399_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPW12071.1"
                     /translation="MPLALVGLTAVVGCRREATPAAAPVAAAPVATPQPAPKPIAPDT
                     KFPTLKLTTLDGQSFDLAAQRGKWAIVNFWATWCGPCLKEMPELSALDAMREHIVVVG
                     LAYEDTTEDDLRAFLVKHPVVYPMALVDVYNPPADFDTPRGLPFTYLIGPDGGVVKKY
                     TGPVSARMLEEDIAAAGGPAAG"
     gene            17047..17322
                     /locus_tag="PK399_02710"
     CDS             17047..17322
                     /locus_tag="PK399_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPW12072.1"
                     /translation="MPAARFIVRGKVQGVWFRASTREQAERLGLDGHARNLGDGSVEV
                     IAIGDAEALDALERWLHVGPPLARVDAVSRAAWPSDPRAAGRGFAIA"
     gene            17401..19194
                     /locus_tag="PK399_02715"
     CDS             17401..19194
                     /locus_tag="PK399_02715"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW12073.1"
                     /translation="MIGKFIGELKRRNVPRAAILYIGSVWALAQGIAQLGPELGAPDS
                     ATRWFLIAAAIGFPLWLVVSWRYEFTSQGIKLESEVDDNALDARKVKRRTDLIIIGVL
                     AIAVVLLLTDRVVERTMPAQAVQVAAAGNSIAVLPFDNQSSDKDQQYFSDGLSEGFII
                     ALSRIDGLRVINRKSSFEFRDSKDDSKAIAAKLGVSHLLEGSVSRVGDTVRIRASLTK
                     AADGSTLWAENYDRPYKDLFALQDQITSQVAAALKSRLQPASAPKAQSDHPPGGNVDA
                     YDAYMQGLSASSMEHAVEAFDRATSIDPAYALAYSRKARLLIDNISAGTLSGKRAEEA
                     YAEADAAIAQAMRLAPDQATTRITRGYLLMLRDFDWRGAEAELKRAVELAPDDGDALY
                     MLGLVRASQGDLAQAVELTRKALQLNPRSADWYRWLVAYLMPLGRLDEAEQAIAKAIE
                     LQPEGIYNHHLLAIAKVLRKDPAAARKAAEAEPAGPWRDFALALAAQAGPDRAAADAS
                     LELVGQYAEGWAFQMAQVYAVRGEPDAMFDWLQRALKARDPGMQSLLYDALLLRYRDD
                     PRYAALAKQAGLPWPPIAAKPAPAPPAPAKP"
     gene            complement(19212..19988)
                     /gene="ppk2"
                     /locus_tag="PK399_02720"
     CDS             complement(19212..19988)
                     /gene="ppk2"
                     /locus_tag="PK399_02720"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HPW12074.1"
                     /translation="MSKLKRKDYEALLQPLQTELANMARWVQHTGQRVVVVFEGRDTA
                     GKGGAINAIREHLNPRQCHVVALPKPTDREAGQWYFQRYAAQLPGAGEIVLFDRSWYN
                     RAGVEKVMGFASDAQVEAFLHATPQFEQQLVDDGILLFKYWLCCDQEQQEKRFAERLV
                     DPLKGWKLSPIDLEARKRYRDYTRAREDMLKATHTRHAPWTLVDFNDQKRGRLALIRD
                     LLDRLPDTAIDAPKIEFPKLAGKPAKERYGVLKPIPVAKA"
     gene            complement(19985..20320)
                     /locus_tag="PK399_02725"
     CDS             complement(19985..20320)
                     /locus_tag="PK399_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017911650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12075.1"
                     /translation="MALDTTLRLLSKASGLTVVDIATAIPRAGVSTVNAWLKGEKLPG
                     KDQVDALARTFGVPAGALLSELASTLDPARTKGEHDLLAAYRSLGSRQQGALLEVARS
                     MKPAKKSTR"
     gene            complement(20323..20841)
                     /gene="moaB"
                     /locus_tag="PK399_02730"
     CDS             complement(20323..20841)
                     /gene="moaB"
                     /locus_tag="PK399_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HPW12076.1"
                     /translation="MTAAADFRPLSLCVLTVSDTRSADNDTSGDYLSDALRDAGHRLF
                     ERAIVRDDKYAMRALVSRWIADGEVDGILVTGGTGFTGRDSTPEALLPLLDKHMDGFG
                     ELFRALSFEEIGTSTLQSRAFAGLANATFLFALPGSTSACRTGWDRIIAAQLDARTRP
                     CNLANLKPRLKE"
     gene            complement(20876..21337)
                     /locus_tag="PK399_02735"
     CDS             complement(20876..21337)
                     /locus_tag="PK399_02735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW12077.1"
                     /translation="MDTTTISRAGPADLDALALLFDGYRQFYGQPTDLPRARQWLRER
                     LRFGESVVLVARRGGGAVGFVQLYPMFSSVRTAKTWILNDLFVEAGARRRGVARALLD
                     AAAAFAREDGAIGISLETSQDNAAARALYRAAGWNEDATQWYSLGFSKLHH"
     gene            complement(21340..22428)
                     /gene="prfA"
                     /locus_tag="PK399_02740"
     CDS             complement(21340..22428)
                     /gene="prfA"
                     /locus_tag="PK399_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPW12078.1"
                     /translation="MTPALRRKLDALVERREEVERLLADPAIAADQTRFRNLSREFAT
                     LQPVADALAAEAQAHADLAAAEAMRGDPELRGLADDEIASATARLQQLEGELLAQLVP
                     KDARDSGGLYLEVRAGTGGDEAAIFAGDLFRMYSRYAEAQGWRVEVESASPGEHGGYK
                     ELVARVEGDGAYAKLKYESGTHRVQRVPATESQGRIHTSAATVAIIAIEPGGDTDIVI
                     NPADLRIDTFRSSGAGGQHVNKTESAIRITHLPTGVVVENQTERSQHANRDKAMKRLQ
                     AMLADAELEKRTAATAADRKLQVGSGDRSQRIRTYSYPQGRITDHRVEGLTLYDLPNI
                     LEGDLDPLVLRLQQEQQAEALARLAQEP"
     gene            complement(22425..23690)
                     /gene="hemA"
                     /locus_tag="PK399_02745"
     CDS             complement(22425..23690)
                     /gene="hemA"
                     /locus_tag="PK399_02745"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPW12079.1"
                     /translation="MALHVLGINHQTAPVSLREKVAFAPDSLAPALASLRALPQVREA
                     VLLSTCNRTELYAHSDDDGQALAGWLSAHPEAGGDLQAYLYRHRDADAVRHLFRVASG
                     LDSLVLGEPQILGQVKQAWSAARQAGALGGQLDRLFQQAFATAKRARTDTRIGANPVS
                     VASTAVRLAQESFARLSDSTVLLIGAGETIELAARHLVEAKAQRLLVANRTLAHAQEL
                     ASRHGGMALPLAELDKHLAEADIVISATASRTPILHRAQVEHALRLRRHRPMLLLDLA
                     VPRDIDSDVSGLRDVFLYTVDDLERAIEDNRRSRREAAQQAEAIIELQTARFVEHWQA
                     SGRQQPLLRLRAHGDAARAEALAKARAQLAAGMPPEQALDLLAHTLTNRLLHAPTVAL
                     RDAALSGDGELARAAEKLFPATGTDSADA"
     gene            23758..25440
                     /locus_tag="PK399_02750"
     CDS             23758..25440
                     /locus_tag="PK399_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW12080.1"
                     /translation="MPIPRHAGPRLLLLAALLAPGLALAASKPSPAKVVVDPMEASLA
                     GEFALQAGQLPEAARQYLQAARSASDPVLAERATRIALLADEDALARDAYATWRSLAP
                     EGGEAQRMVAAGLALRAGDKRASRRELRTLLAEGGKGWRSALAALFGAVGKQQKLVVA
                     MIDGVVDDNLLPDDLQAWLGFGGLAQRLEQPALVDRIVGEIVRRYPGEPRVALLRAQL
                     LREGGKLGEARTLLAGLEDAAKLSPQLRWSVAGEYDALGDSAKAAQVLGWGPQDDASH
                     AQRAMLLDKAKDKAGLAALYEELKREATHPNPMRRLLLGQLAELLERYEDARGWYANV
                     PGEGVQGIARLRGANVLHAMGRKPAAFDELHAIQADQTLDEEARRDGYLLEAELRLKD
                     KDEAGELDAYARGLAAFADDEALLYSRALLWERRDEIAKAEADFRRILVISPDDVNTL
                     NALGYTLADRTTRYREALELIDRARVAAPGNAAIIDSYGWVLFRLGKPRQALDHLRHA
                     YALQKDADIGSHLGHVLWVLGERDEARKYFEEARKLDPENRSLQRALQATGA"
     gene            25437..26102
                     /gene="lolB"
                     /locus_tag="PK399_02755"
     CDS             25437..26102
                     /gene="lolB"
                     /locus_tag="PK399_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HPW12081.1"
                     /translation="MNRRIALAALGMAVLAGCTGVPVARPAAPGQVVGTDADSPQAMA
                     QRQRIERLGLADGDCSAPAWALTGRVALSNGKDGGSGRITWSQAAGEARIELTAPVTR
                     QGWALDVDADGATLQGVPGGPVHGADAAALLRERTGWDIPVAGLGCWVRGAWASEAAF
                     GLATVGYGEDGRVQRIEQAGWTIDYADWKPDAASGADLPARITALRNTDRVRLVVDRW
                     AVD"
     gene            26111..27019
                     /gene="ispE"
                     /locus_tag="PK399_02760"
     CDS             26111..27019
                     /gene="ispE"
                     /locus_tag="PK399_02760"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPW12082.1"
                     /translation="MADFPATEGWSRWPAPAKLNLFLRITGRRPDGYHALQTAFRLLD
                     WGDVIHLRRRTDGVIVRVGAGADGVAEADDLMVRAAKLLRKETNCRQGADIGIEKNIP
                     TGGGFGGGSSDAATVLVVLDRLWNLALGVDRLAALGLQLGADVPVFVRGHNAWAEGVG
                     EVLTAIELPPAWYVVASPGVHAATAALFQAPDLTRNAAPAKITGFLSGTSPSGAVLDN
                     AFEPVLRRREPAVEALFAQLASIGTPRLTGSGSGCFVEFAQRESAEAALASLPPGIHA
                     WLARGAERSPLLDAVEGFNAGASPSG"
     gene            26998..27073
                     /locus_tag="PK399_02765"
     tRNA            26998..27073
                     /locus_tag="PK399_02765"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27031..27033,aa:Gln,seq:ttg)
     gene            27178..>27550
                     /gene="prs"
                     /locus_tag="PK399_02770"
     CDS             27178..>27550
                     /gene="prs"
                     /locus_tag="PK399_02770"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020852075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPW12083.1"
                     /translation="MQDDRNLLVFSGNANRPLADAVCRELGVRPGKALVSTFSDGEVQ
                     VEIEENVRRQEVFIIQPTNAPTAENFMELLVLVDALKRASAASVTAVVPYFGYARQDR
                     RPRSARVPITAKVAAKMIQAVG"
BASE COUNT         4279 a         9201 c         9592 g         4378 t
ORIGIN      
        1 gccgtgcacc gcattggtcg gccagccgtc ggcatcgtgg aactcgtccg gcatcgaatg
       61 ccaggcgcgg aacacgtaca tcgaggcgtc gaccaggtgg agggtgggtt gggtcatccg
      121 gtcctacagg gtgaggtccg tcaggaggtc gcgcggatcc gggcgctcgc ggtcgggcac
      181 atcgacctgc ggggtgccca tggcgatggt gccgaccagt tgctcgtccg cctgcagccc
      241 cagcgtgttc tcaaggaagg ggcggtcgta gcagggccag ccggtcagcc attgcgcgcc
      301 gtagccttcg gcctgcgcgg cctgcagcag caacaggcag acacaggacg ccgtcgaaaa
      361 ccgttccgag gcagggatct tctcgtcatc gcccagcctg gcgatgaccg cgatggtcag
      421 cggcggcagg gtgaagcggt tgcgcagttt ctcgatggcg ccttccgcag gcctggattc
      481 gcgttgcagc aggcgggcga tggtggcgtc ggccagcgcc ttgcgatcat cgccggcgat
      541 gcgcaggaaa cggaacggca cgcgcttgcc gtggtcgggg acgcgcacgg cgctgcgcag
      601 gatccgcagc agggtgtcgt ggtcggggcc gggtgcctcc agttgccggg tcggcaccga
      661 caggcggctg tcgagggcgg caagggtgag cgtggtcggg gaagtggtca tgtcgtcatg
      721 gtaatggtgc ctgcctgcat ccggccggca ttcggtagga tgctggcatc acggcgaggg
      781 agcgggcgat ggcggtgaat attggcgtgg cgcgcgagag cgttgcgggc gaacggcgcg
      841 tggcgttgac ccccgagacc tgcaggaagc tggtggcggc gggcgccacg gtgcggatcg
      901 agtccgggat cggcgatggc gcgtatttcc ccgaccaggc gtatgtcgat gccggcgcgc
      961 acgtcaccgc gcacccgcgc gaaggtgccg atatcgtctt gtgcgtccag ccgccgtccg
     1021 ccgccgcgat cggttcgctg aagtcgggcg ctgtcctggt cggcagcctg cagccgcagg
     1081 ccgaccccgc gcgtggcgaa gccatcacgt cgcgcggcat cgtcgccttc ccgctggaaa
     1141 agctcccgcg caccacccgc gcgcaggcga tggacgtgct cagctcgcag gcgggcatgg
     1201 ccggctacaa ggcagtgctg atcgccgcgc aactggcgcc gcgcttcttc ccgatgctga
     1261 ccaccgccgc cggcaccatc cgtccgtcca aggtgctggt cgtcggcgcg ggcgtggccg
     1321 gattgcaggc gatcgccacc gccaagcggc tcggtgcggc ggtggaaggc ttcgacgtgc
     1381 gtccggagac ccgcgagcag atcgaatcgc tgggcggcaa gttcctcgac ctcggcgtat
     1441 ccgcggcggg cgagggcggt tacgcccgtg cgttgaccga cgaggagcgc gcgctgcagc
     1501 agcagcgact ggcggatcat ctgaagggca tcgacgtggt ggtctgcacc gccgcggtcc
     1561 ccggtcgtcc ggcgccgaag atcatcagcg cggcaatggt ggccgggatg aagccgggca
     1621 gcgtggtcgt ggacctcgcc gccgagaccg gcggcaattg cgagctcacc cagccgggcg
     1681 agacgatcga gaaggacggc gtgaccatcg ccggcccgct caacctggcc tcgatggggg
     1741 cgcagcacgc cagcgagatg tacgcccgca acgtctacaa cttcgtcagc ctgctgctca
     1801 aggacggcgc gctggcgttc gactgggacg acgaactgct ggcgaaaacg gtgtggccgg
     1861 agcgggtggt tgctgcgtaa gtcgctggcg atcgccgcgg cggcgatcct tgcgatctac
     1921 ctggggatct gcggcctgct gtacgggtcg cagcgcgcct acctgtactt cccggtgccg
     1981 cggcgtgccg aggtgccgtc gttcgtcatg acatccggcg atgccgacgt ggtcgtttcg
     2041 acgaatggcg tcgacagccc gcaggccgtg gtctatctcg gcggcaatgc cgaggacgtg
     2101 tcgcaggccg tcgcgctgct cgcgacggcg ttccaggggc gtgcgatcta cgcgatgcat
     2161 taccgcggtt acggtggaag cactggctcg ccttcggagc acgcactggt cgcggatgcc
     2221 ggtcgcctgt tcgaccaggt cgcgacgaat catcagggca tcgtggtggt ggggcgcagc
     2281 ctgggcagcg gcatcgccct gcaggtcgcc gccacgcgtc gcgccgagcg gctggtactg
     2341 gtgacgccgt acgccagcat cgccgaactc gccgcggcgc agttcccgtt gttcccggtc
     2401 ggcctgctgt tgcaggacag gtacgagtcc tggcgatacg cgccacggat caaggtgccg
     2461 accacggtca tcgtcgcgga tcgagacgag gtcattccca atgccagttc gcggcgcctg
     2521 gcggaatgtt tcgtgcctgg catcgcgacc gtggtcaact ttccggatgc cgggcacaac
     2581 gatgtatcgg acgcgcccgg ttacccggcg gcgctcgccg gatccggccc tgcgacgacc
     2641 gagtgagcgc ctagcgttcc cgcttctccg gcgctggatg cgccttcagc cagtcggcct
     2701 tctgctgcgg cgtcttctgc cgccactccg ccatgaacgc cttgcgctgc tccttgtcca
     2761 ggccacgcat ggcatggaac agggcgcgcg cttcgccacg ctgctcgggg gacatgtgtt
     2821 cccagcgctg catgccgtgt ccggcgcgct cgcgctggtc gcgcggcatc gactgccagc
     2881 gctgcgcacg ttcggccatg cgcgcgcgtt cgtccgggtt gcggttccag cgctcgcgca
     2941 ggggagcgac cagggcgtcg cgctgcgcct gggtgagttg gtcccattcg ggatactggt
     3001 tgggaacggc ggcggactgc gcgaaggcga acgcaggcca cagggccagg gcgaggatgg
     3061 tgaggcggat cgtgcggttc attgtttact tactccatcg cgatctgctg ggcatccgcg
     3121 gatgccagcc atgcgtagaa ctcggggtct tcatcgagca tggtggtgtc gggcgtcgcc
     3181 gacgtggagg cgatgccggg ttcgaggctg gccggcgccg gcatgcttcc gaactgcagg
     3241 ccgatggcca gcgcgcagag tgcggcgaaa cctgccgccg cgtagcgcag gccgacgcgc
     3301 gccggcgcga ccgcctcacc gcgcagggcg gcgttgcggc gctgcgcgag ttgcgcgcgc
     3361 acccgcggcg acaggcgatc gaccgcgacc gcatgcgtgc gacgcacggc ctcgtcgaaa
     3421 cgggcatcgc tgccggtgtg gtcggaatgt gtattcatcg gaactcctcc agttgccgct
     3481 gcagcgcctc gcgggcgcgc gaaagatggg tcttgaccga gccttcgctg cagcccatgg
     3541 cgcgggcggt gtcggccacg tccagttctt cgagcacgcg cagggtgaac gcctcgcgct
     3601 ggcgcgtcgg cagtgtccgt agcgcttcgc tgatccgcga ccaggcctcg cggctgtcgt
     3661 ggtggcgcga tggatccggc ccgtcatcgg cccagtccac cggctcgccg tccttgtcgg
     3721 tgctgcccgg catcagccaa cgcagccgga acagcccgcg ccgctggatg tcgatgatgc
     3781 ggttgcgcag gatgctccag aacagcggac tccactcggc cggcggccgg tcgcggtagc
     3841 cgagcatctt catcatcgag tcctgcaccg cgtccagcgc gtcctcgcga tggcgcagcc
     3901 cgagctcggc gaagcggaag gcgcgcccgt cgatccccgc aaggaattgt tccagcgacg
     3961 tgggcagggg cgccggggcg gcaaccgcca cgggctcaag ttcgttgtcg ggggcctctt
     4021 ccaccgggcc tagcttagcg gcgacgaaca gcatgtcggg cactctcggg gcgggggggt
     4081 gccggggcgg gcacgggaag gatcggcggg attcacgaca tgcgaaacgc ccatgtggcg
     4141 cgtcggttga cagcgggcac aagcgcgcgg gccgtggtta tgatgcgggc aggcgatcgg
     4201 ggagaggctt cgtgggcgac ggattcgtgg cgctgtacat cttcatgctg gcggcgatcg
     4261 ccggcgacgt gatcatctcg cgggtgccgg tgatcctgca taccccgctg atgtccggct
     4321 ccaacttcat ccacgggatc gtcctgatcg gcgcgatggt ggtgctggga cacgccgaca
     4381 ccccggtgga gcaggcgctg ggcttcgtcg cggtgctgct gggcgcgggc aacgccgccg
     4441 gcggctatgt ggtcaccgag cgcatgctgg agatgttcaa gccctcgcag aagacggagc
     4501 cgaaggcatg agcctgctgg tgctggtcaa gctgagttat ttcgtcgcgg caacgctgtt
     4561 cctgctcggc ctgcagcgca tggccagccc caagaccgcg cgcagcggca tccagtgggc
     4621 cggcgccggc atgctgatcg cgactttcgc cacgttcttc ctgcccggcc tgcacaacat
     4681 cggcctgatg gtcgcggcga tcgtcattgg cgtggccgcc aactgggcct ggggcaagaa
     4741 ggtggcgatc accgacatgc cgcagatggt ggccctgttc aatggcatgg gcggcgggtc
     4801 cgcggcggcg atcggcgcgg tggaactggt gcgctggtcg gcggccgggc aatcgccggg
     4861 gatggtgccg ggcgtgctgg cggtactcgg gtcgctgatc ggcgcggtct cgctgaccgg
     4921 ctcgatcatc gcctgggcca agctcgacgg gcgcatggac aagcgctaca ccttccccgg
     4981 ccagcagtgg ttcaacgcgg tgatcgcgct ggcggccgtg gtgtgcgggg cgatggccct
     5041 gcagaccctg gcgatgccct ggatcgtggc gttcttcgtg ttggcgctcg ccctgggcgt
     5101 gctgatgacc ctgccgatcg gcggcgccga catgccggtg gtgatctcgc tctacaacgc
     5161 cttcaccggc ttggcggtgg cgttcgaggg ctacgtgctg ggcaacgagg cgctgatcat
     5221 cgccggcacc atggtcggcg cggccggcat gctgctgacc cggctgatgg ccaaggcgat
     5281 gaaccgcaag atcagcaacg tgctgttctc cgacttcggc ggcggcagtg gcgagatgca
     5341 ggagatcggc ggctcgatga agccgatcga ggccgccgac gtggccgcga tgatggcgta
     5401 cgccgagcgc gtggtgatcg tcccgggtta cggcatggcc gtcgcgcagg cccagcacaa
     5461 gatctgggaa ctgagccagc gcctgatgga gcgcggggtc aaggtgaagt tcgcgatcca
     5521 cccggtggcg ggccgcatgc ccggccacat gaacgtgctg ctcgccgagg ctggcgtgcc
     5581 ctacgacctg atcgccgaca tggacgacat caacccggaa ttcgccaaca ccgatgtctc
     5641 gctggtgatc ggcgccaacg acgtcgtcaa cccggtggcc aagaccgacc cggccagccc
     5701 gatctacggc atgccgatcc tcgacgtggt caactcgaag aacaccatcg tgatcaagcg
     5761 cggcaagggc accggctttg cgggcatcga gaatgccctg ttctacgccg acaacacgcg
     5821 gatgctgtac ggcgatggtg ccggcgtggc cagcgcgctg gtcagcgaac tgaaggccct
     5881 cgacggcggg cattgaggcc cgccaccagc gccaccagca gtccggacgc ataaaaggcg
     5941 agatccagcg gcgagttcgc catctccagc aacgtccagg cgtggttggc gcccagcatc
     6001 agcgcggatt cccacggacg caggccaaag ccctggccca gttcgccggc ggcgatcccg
     6061 aagttcgcca ggacaatcgc catcgcggtc gccgcgaatg cccaacccgc gcgcgttgca
     6121 cccgcgggcc aacggctcaa ccccagcagc agcaccatgt ccagggccgc aagtggcgcc
     6181 agccatgcgc attgccgctc cagggccagt gcagccagca cccaggcgca gacgaatccc
     6241 agcatgccca acgccagcaa gggcagggcg aaccagcgcg gcgtcgtggt tgggtcggtg
     6301 gtcatggtcg ggccgcggcg gggtcgcgca tcatacccag ccggcaaagg gactagaatg
     6361 gtacggattc caccgtcccc tcttgcttgc ccatgtattc ccgcagcagc gaacccgtcc
     6421 gtttcgagcg cgattgcgcc gccgtcctgg tgccgcaggg cgatgaagtc acccttcccg
     6481 cggggaccgt tggctacatc acccagtcgc tgggcggcag ctggaccgtg ttcgtggaag
     6541 gcaacctgat gcgcatcgcc ggcaaggatg gtgatgccat cggcaaggaa ccgccggcgc
     6601 cgatcgcatt gccggaaggc gccagcgacg aggacgtgga agcgctggtc tggcagcagt
     6661 tgcggacctg tttcgacccg gagatcccgg tcaacatcgt cgaactcggc ctggtctatt
     6721 cggccgaggt gcgtccagat gacgaaggcg ggcgcgtggt ggaagtgcgg atgaccctga
     6781 ctgcgccggg ctgcgggatg ggcgaagtcc tggtggacga cgtgcgcagc aagctggaga
     6841 tgatccccac cgtcgccgaa gccgacgtcg aactggtgtt cgacccaccg tggggccgtt
     6901 cgatgatgtc cgagctggcc aagctcgaaa ccgggatgct ctgaccgcgg gtgccaaccg
     6961 aaagttgacc ggtgtcactg cgtttgtgac catcgacaca ggcccgttgc gtcacttttt
     7021 gccccagtca gtcagcgcaa ttccgtgttg ccacgcagca gaattgctgc gccaaacccg
     7081 ttcagggttg tcgatgctgc gctgaagcaa tcgctgcgat ggctctggaa cgccattgac
     7141 tacgtatgca ggatcgcata ttgccgatcg ttatatgcat attcccgacc tcgcttgacg
     7201 gtcgatggtc tccgccgtaa ctgtccgatt cgcttcggga acggttcagc ttgccggggc
     7261 gctccgcgac tcattagacc tcctgcagga ggcaacgcgg gcagcaagag ttgccaaccc
     7321 atccgtcgcg ctgcgcggcg atccaaaaac atcaccacta ggggtagtgc cagtgaatcg
     7381 tttcagccga atcaacatgc tcgccaccgc catcgcgttg tccctcgtcg cgaccggcgc
     7441 acaagcagcg ggcaaggtcg acctgcacaa gcgcgacatc gcccagctca agcagcagta
     7501 ccagtcccag gtcgccgccc gcggcgtggc caagatggcc cacgcccgcc acgcgcagtt
     7561 catgcgcgcc gatggggaca gcaccctgct gatgcgcgcc cagcgcgccg actacggcgt
     7621 gcgcaactac cgctatgacc agaccttccg tggcatcccg atcttcggcg aaggcgtggt
     7681 ggtcagcgag gacggcgccg gcaacgtgcg caccatgttc ggcaacctgt actccggcct
     7741 ggaccaggac atcgcctcga ccacgccgcg cctgagcaag gcggccgcac tggtcgccgg
     7801 caagcgcgcc ggcctgggcg accgcatctc cgggatgctg gtcgagaacg aatcggccga
     7861 cctgcgggtc tacatcgacg aatccggccg tggccacctg gcctacgtgg tgtccttctt
     7921 cgccgacgcc atcggcgcgg gcagcccgac ccgtccgctg atcatcatcg atgccaacac
     7981 cggtcgcatc ctcaagcagc gcgagaacct ccagcatgcg ctggtcggca ccggccccgg
     8041 cggcaacgcc aagaccggcc agtacgagta cggcaccaac tacggttacc tggacgtcac
     8101 gcagagcggc accacctgca cgatgaacag caccaacgtg aagacggtca acctcaacaa
     8161 cgccagcacc aacaccagca acaccgcgtt ctcctacacc tgcccgcgca acacggtgaa
     8221 gaccatcaat ggcgcctatt cgccgctgaa cgatgcgcat tacttcggcc aggtcgtctt
     8281 caacatgtac caggcctaca tcggccaggc accgctgagc ttccagctgg tgatgaaggt
     8341 ccactacaag accaactacg aaaacgcctt ctggaacggc accgcgatga cgttcggcga
     8401 cggcgccagc acgttctatc cgctggtcag cctggacgtc agctcgcacg aggtctcgca
     8461 cggcttcacc gagcagcagt ccggcctttg ctacgacaac ggcagccagt gcggcggcat
     8521 gaacgaagcc ttctccgaca tggccggtga ggccgccgag tactactcgc gcggcaccaa
     8581 cgactggaag gtcggcgccg agatcttcaa gggcaccggc gcgctgcgct acatgaacaa
     8641 cccgacgcag gacggctcgt cgatcgacaa cgctgcgaac atgaccagca gcatggatcc
     8701 gcactactcg tccggcgtct acaacaaggc gttctacaac ctggccacca aggccggctg
     8761 gaacacgcag aaggccttcc aggtgttcgc ccgcgccaac cgcgattact ggacggcgaa
     8821 catttcctgg aacaacggcg cctgcggcgt gcagacggcg gcgaccgacc tcggctatac
     8881 cgtggcggac gtgacctcgg cgttctccgc cgtgggcgtg agctgctcgg gcggtggcgg
     8941 cggtggcggt ggcggcagca ccggtggcgt gctgaccaag ggcgtcgcgg tgaccgggat
     9001 caaccagacg gcgggcaacg cggtgaacta caccatggtg gtgccggccg gttccaccaa
     9061 cgtgaccttc accatgtccg gcggcacggg cgatgccgac atgtacgtga agctcggcag
     9121 cgccccgact gattcgtcat acgactgccg tccgtacaag tccggcaatg ccgaatcctg
     9181 caccttcgcc agctccaccg gaggcaccta ctacatccgc ctgaaggcgt actcggcgtt
     9241 ctccggcgtc agcctggtcg gcgactacac cgccggcggc ggtggcggcg gtggcagcac
     9301 ccagacctac accaacacca ccgactacaa catcccggac aacaacgcga cgggcatcac
     9361 cagcacgatc aacgtctcgg gtcgcaccgg caacgcgccg agcaacaccc aggtggcggt
     9421 gaacatcgtc cacacctaca gcggcgacct ggtggtcgag ctggtggcac cggatggtac
     9481 ggtctacacc ctccagagcc gcgttggtgg cagcgccgac aacatcgtca agaccttcac
     9541 caccaacctg tccagcgaag cgatcaatgg cgcgtggaag ctgcgcgtga aggatcgtgc
     9601 cagcacggat accggctaca tcaacagctg gagcatcacg atgtgatccg cggtccgcaa
     9661 ggaccactga tccgctgatg catcgacaac cccggttcgc cggggttgtt tttttccacg
     9721 aaccgaaatg ccggcgcgcc tgcgcaccgg ccaggacagg agtgtctcca tgatccggaa
     9781 aagactgctg caaggcctgt tgcttgcctc cgccctggcc ggcggagcgg cgttcgcggg
     9841 cgagggcgcc accgcgcatg cacgggcgca tgccagcgcc gaactggcct cgatgcacct
     9901 tgaggccaat cccgacgcca cgatctacat cgtcacctcg aacaacacct accagcgcgt
     9961 tcgcggaatg acccgcaacg gtgtggcccg ttcggatagc ctgggccagc cgctggtcct
    10021 ggcggagatc cgcgcagggc agttgagcgc gctcagcgaa gacatccaca cgatggaaaa
    10081 gcgctgcggc ggctacttcg ccttcgccac gcgcgcggcg gcggaagctt tcctgcgtgc
    10141 ggaccgcgcc gcggctgcag tcggaaggtc gctggcggcg agctacaccc tcgacaacca
    10201 ggccaccgtc ggtccatggc tgtcgcaggt cgcggcggcg aacatccagc agaccatcac
    10261 ccagttgcag ggttaccaga accgctacta cagcagcccg accggcaaga cctcggcgga
    10321 gtggatccgc acctcctggc aggcgctggc gaacggtcgc accgacgtca cgaccgaact
    10381 gttcgcctgc agcacatgtt ccacccagcc ttcagtgatc ctcaccatcc agggcaccga
    10441 cctgccgaac gaagtcgtcg tgctgggcgc acacctggat tcgatcagcg gcagcggcgg
    10501 caacacggtg gtggctccag gcgcggacga tgatgcgtct ggcatcgcca ccctgaccga
    10561 gaccttgcgg atcgccttgg ccaacggctg gaaaccgaag cgcacggtca agttcatggg
    10621 ctatgccgcc gaggaagtgg gcctgcgcgg ttccaatgcg atcgcgacct cgttcaggaa
    10681 cgcgggcacc aacgtggtcg gcgtgctgca gatggacatg accaactacc agtcgggatc
    10741 ggccaccgcc atgcgcctga tcaccgacta ttccaacgcg gacctgaaga ccttcttcaa
    10801 ccagctgttc gacgcctacc tgctgccgct cggttacacc cgcggcacct acacctgcgg
    10861 ctacggctgc tcggaccacg cctcgtggac cagtgccggc tatccggcgg cgatgatgtt
    10921 cgaggccggc accgccggcg gcggcatgaa tcccaacatc cacacgacgg ccgacaccct
    10981 ggccaacatg ggcaacaccg cgcagcccag cgtcaagttc gcccagttct cgctggcctt
    11041 cctgggggag ctggccaaga cccagggcac gggcggcggc agtggcggca cgggcggcgc
    11101 gctggcgaat ggcgtggcga aggcgatctc gggcaccacc ggcagcagcc aggtgtggac
    11161 gctggcggtc cctgcgggcg cgaccagcct gaagttcacc accgccggcg ggtccgggga
    11221 tgccgacatg tacgtcaagt tcggcagcgt gccgaccacg accagctacg actgcaaggc
    11281 cgatgccagc ggcaatgccg gcacctgcag catcgcgacc gcacaggccg gcccctacca
    11341 ggtgctgatc aagggctaca gcgcgttctc gggggccacg ctgaccggca gctacgccac
    11401 tgggggcggt ggcggcagca cccagaccta cagcaacggc accgacgtca ccatcaccga
    11461 caacaacgcc accggcgtga ccagcaccat caccgtctcg ggtcgtacgg gcaacgcgcc
    11521 gagcaatacc caggtggcgg tgaacatcgt ccacacctac agcggcgacc tggtcgtcga
    11581 cctggtcgcg ccggatggca cggtgtacaa cctgcagaac cgcagcggcg gcagcgcgga
    11641 caacatcgtg aagaccttca cgaccaacct gtccagcgaa acgatcaacg gtgcgtggaa
    11701 gctccgcgtg gccgatcgcg ccgcgacgga caccggctac atcaacagct ggtcgatcac
    11761 gatgtgattc atggggcagg cgggatcgcc cgatcccggc gagaaacgaa aaccccggct
    11821 tcggccgggg tttttcttgc gcgtcgaggc gatggagatc gcgccgggga cgatcagccc
    11881 agcgcctcga agcggttggc gtccacgttc ttgcgcacct tcagcggatc ccagatgcgc
    11941 ccgttcatgg cgatgtaggt gcccggcggc agcgactgca ccgcaccgac cgcgcagccg
    12001 atgttgaact ccgcgtcgga gccctcgaag cgcgccgggc tgagcgcgcc ggtcagcacg
    12061 atggtcttgc cctcgatgcc ggcgagcacc cgcgcggtct cgaccatgct gtcggtgccg
    12121 tgggtcacca gcacgcaggc ctcgtcctgc gcggcgatgg tggcgcgcag cagctggcgg
    12181 tcggagtcgt ccacgaacag cgaatccttg cgcagcagcg ggatcacgtg gaagcggaac
    12241 gccacgccga gttcctgcag catgcggccg atctgcgggt cgccgacctg gtagtcggat
    12301 ttcgcgtcga agtacacctt gtcgatggtg ccgccggtgg tgacgatcag caggttgtcc
    12361 atgcgttgat tgtccgccaa aaccacccgg cgcgacacca ggtcagcggc ggtcccggcc
    12421 gagcgcggcg cgcagcggat caagcagcgg cccatacgcg gacgcacggg cggtgtcgcg
    12481 acgcaagggc tggcgcacct gcgcggcgct ggcggtgcgc acggggcggc gcgtttcatg
    12541 cgaggccagg caggcggagt cgaagcgcag gccgcagtcg tcgagcaggg cgcggatccg
    12601 tggctccgga tcggccagca ggtcctcgta gcgatgcagc ctgatccgcg ccgggtcgcg
    12661 cgcgcggaac gcgtccatcg cgccttcgct gccgcggatg tgcgcggcga tgtcgccggg
    12721 gtcgttggcg aaatgcggct ggctgtagaa ctgctgcttg aagcacgacc acgcggtttc
    12781 cagcggatcg cggcgggttt cgatgacagt ggcacccggc aacatcgcgc gcaggatgcc
    12841 ggcgtgcttc cagttctccg gcagcttgtc ggtgaagcgc ggcttgtcgg cgcgccagcg
    12901 tgcggtgcgg tcgagatagg cgcgacccag ccggtgccag tcgtcagccg tggcctgcgg
    12961 cacccattgc ggatacgcca cgccgcgacg catcgattcc tgctggatca cgatgccgag
    13021 gtcgggcaat tcgctggcac cttccacctc cggatgcgcc gccagcacct gctcgaacag
    13081 ggtggagccc gagcgcggct ggccgacgat gaagatcgcc tcccggccca actccggatc
    13141 gagcggcgag ggcaagacct ccgatgccgc aagcgctcgt gccacgaatt gctcgaacgc
    13201 ctgccggctc cacggcgtca gccgcttctg caggtcgttc gcggccacga aggccgcatg
    13261 cgcatcggcg tggcggccct ggtcttcgtg cagcttgccg agcgcatggc ccatggccac
    13321 gcggtcgctg tcggcgatgt cgcggcgttg cagttgtgcg gcaagcgcac gtgcatcttc
    13381 atcgtcgagc gcgcgggtct tgatgttgga cagcccgcgc cacgcatcgc cgcaggcggg
    13441 gtgcaggcgc agggcatcgc ggtactgcga tgccgcctcg tcgaaatctc cggcatgcac
    13501 cagcgcatcg cccagcagga tcttcgccgg cagcatgtcc gcggcgnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnggca tgcaccagcg catcgcccag caggatcttc
    13681 gccggcagca tgtccgcggc gatcgcgttc acctgccgaa gcgcggcgat ggcctcgtcg
    13741 gtgcgaccca gttgctgcag gttgcgcccc aggttgaacc acggcatcgg ctgtgcaggg
    13801 tcgagttcgc acgcacgccg ccagctcgcc aatgccgcgt cgatgtcgcc gctcgcggac
    13861 tgcgcggtgc ccaggtcgca atgcaacagc gcatcgccgg gtgccagtgc caatgcgcgc
    13921 tgcagggttt gcgtcgccaa cgctgcgcgg cgtgtgtgca gttgcaggat cgccaggtag
    13981 cgcagcggct ccgggtgcgt ggctgcctgc atgcaggctt gcatcagcag ctgctccgcg
    14041 cgcggcggct ggccatcgcg cagcgcgcgc gcgccggcat gcacgagctc gaccacggcc
    14101 cgtggcaggc cggtcatccg cgggtcggcg ctcatcctgc gttgcgccgc ttctcgcgcc
    14161 gtgcctgcat gccgggcagg caggaggcat agatgaccgc aagcgccagc gcgacttcga
    14221 gcagggccat cccgcgttgc gccggctcgg accaggccag catcaccccg tgcgagaacc
    14281 acagcagggc gagcacgccg gcggcgaagc cggcacggtg ccagccgcgc catgcggcga
    14341 tcgccagcag gaacggcggt agcgcgaaca ccagcaagcc ggtcagcagg tgttcgtcgc
    14401 ccgcgaacca ggccgcgtag aacaaggtga gcgcgagtag cgcgaaggcc agcggccgca
    14461 gcgccttcat gcctgcaacc gccgtgccag gtcggccacg cgccggccca gcgcgcgcgc
    14521 caacgcggct tcgtcgtcgc tcagttccgg atcgtcctgt ccaccggcca cgtggctggc
    14581 gccgtacggc gtgccgccgc tgcgggtggt attgagcgca gattcggtga acgggatgcc
    14641 gcagaccacg cagccgtggt gcagcagcgg gaccagcatc gtcagcaggg tcgattcctg
    14701 gccaccgtgc atggtcgcgg tggaggtgaa ggccgctgcc ggcttgccga ccagcgtgcc
    14761 gctggcccat tccgcgccga gtgtgtcgat gaaatgcttc atcggcgccg ccatgttgcc
    14821 gaaccgggtc gggctgccga gcagcaggcc cgcgcattcc gccaggtcac gcttgtccac
    14881 gtagggggcg ccgtcttcgg gttctggcgg gctggcgatc gtggtggtcg gcgccaccgg
    14941 cggcaccgtg cgcaggcgcg cctgcatgcc atccacttcg ccgatgccgc gcgcgatctg
    15001 ccgcgcgagg cgcgccaccg agccgccacg gctgtaatag aggaccagga tctcgggcat
    15061 ggcgattgca aaaaggggcc agagtgaagt ttcgcacgga gcttctccac gaatcgcgga
    15121 tgccggtgcg aaattcactc tccccccttt ttgctttctt gcgaagcgac tcaaacaggg
    15181 tatcggttag gcttgggcga tggagccgct ggattccctg aaccgctgga acgaccgcct
    15241 gcgagatcgc gcgcgcgcgg gcagcttcgg ccgcttcgtg ctcaagcgct tcctcggcga
    15301 ccgcctgttc gaggccgccg gcgcgctgtc ctacaccacg gcgttcgcgc tggtgccttt
    15361 gtcgatggtc gtgttcggag tgctgtccgc gttcccggtg ttcgatcatt ggagccagag
    15421 cctcaaggcg tacatcttct ccaacttcgt gccaagcgcc gcgagtgcgg tgtcgggtta
    15481 cctcaccgat ttctcggcga acaccaagtc cctgacggcc gccggcgcgc tcgccctgat
    15541 cgtgtccttg ctggtcacgc tgaccagcgt ggaggcggtg ttcaaccgca tctggcgggt
    15601 gcccaccgcg cgacccaagt tcggccgctt cctggtgtac tggaccgtgc tcaccctggg
    15661 tgcgctggtc gcggccacga gccttgcgtt gctcacgcgg ttctttgcgc tggcgatctt
    15721 cgagaccgtc ccggggcgat ggctggaggg gttgatgctg cgggtgatgc cgatggcgat
    15781 cgagttgctc gccttcgccg cgatcttcaa ggtggtgccg caccgcaccg tgaagtggcg
    15841 gcatgccttc gccggtgcgg ccttgtcggt gttcctgttc gaatgcgtga agtcggggct
    15901 ggccctgtac ctgggcagct tcaactcgta ccagacgatc tacgggacgc tcgccatcgt
    15961 gccgatcctg atgctgtgga tcttcctggg ctggacctcg atcctgttcg gcgcctcctt
    16021 cgcctcgtcg atgtcggcgt tccgctacca accgttggcg ctgcggttgc cgcacggcta
    16081 cgagttgtac gggctgttgc gcatgctcgg tcgcttccag caagccaggt ccgcgggaaa
    16141 gggcctgcac agcgacgaaa tccagcaact ggaaccgatc ctcaccgaca gcctggtgca
    16201 ggaactcctg gggcagatgg cggcgatcaa ggtggtcagc cgtgccgaag acggccagtg
    16261 gctgctcgcg cgcgacctgg acgacgtcag cctgggcgaa ctctacgaag ccgcgcacct
    16321 gcgcgtgccg gtcgccgagg cgcgcctgcc gcatcacgac gatgcgctgg gcgtcgcggt
    16381 gatggaggtg atcaacggcc tgcgcatgcc gctgcgcgac ctgctcaagc agcgcgtgtc
    16441 ctgcgtctat gaacgcatcc ccggagaatg aattgaacac gttgcgcaac ctcttcatcc
    16501 cgctcgcgct ggtcggattg accgccgtgg tcggttgcag gcgcgaggca actcccgcag
    16561 ccgcgcccgt cgcggcggcg cccgtcgcga caccgcagcc tgcacccaag cccatcgcgc
    16621 cggacacgaa attcccgacg ttgaagctga ccacgctcga cggccagtcc ttcgacctgg
    16681 ccgcgcagcg tggcaagtgg gcgatcgtga atttctgggc gacctggtgc ggcccgtgcc
    16741 tcaaggaaat gccggaactg tccgcgctgg atgcgatgcg ggagcacatc gtggtggtcg
    16801 gcctggccta cgaggacacc acggaggacg acctgcgcgc cttcctggtc aagcatcccg
    16861 tggtctatcc gatggcgctg gtcgacgtgt acaacccgcc ggccgatttc gatacgccgc
    16921 gcggcctgcc gttcacctac ctgatcggtc ccgacggcgg ggtggtgaag aagtacaccg
    16981 gcccggtcag cgcgcggatg ctcgaagaag acatcgccgc cgcgggcgga cccgcggcag
    17041 ggtgagatgc cggcggcgcg gttcatcgtg cgcggcaagg tgcagggtgt gtggttccgc
    17101 gcctccaccc gcgagcaagc cgagcggctt ggactggacg ggcatgcccg caacctcggc
    17161 gatggcagcg tggaggtgat cgcgatcggc gatgcggagg ccctcgatgc gctggagcgc
    17221 tggctgcacg tgggcccgcc gctggcacgg gtcgatgcgg tgtcgcgtgc tgcgtggccg
    17281 tcggatccac gggccgcagg ccgcggcttc gcaatcgcct gacccttgca tgagcctgtc
    17341 ctgcaccgac ttgcgccttc gttatgcaag aatcgggcag gggatgccac agggcgcggc
    17401 atgatcggca agttcatcgg ggagttgaag cgccgcaacg tgccgcgcgc ggcgatcctc
    17461 tacatcggtt cggtctgggc gctcgcgcag ggcattgccc aactcgggcc ggaactgggc
    17521 gcgcccgact ccgcgacccg ctggttcctg atcgccgccg cgatcggctt tccgctgtgg
    17581 ctggtggtgt cctggcgcta cgagttcacc agccagggca tcaagctcga gagcgaagtc
    17641 gacgacaacg ccctggacgc gcgcaaggtc aagcggcgca ccgacctgat catcatcggc
    17701 gtgctggcga tcgcggtggt gctgctgctg accgaccgcg tggtcgaacg caccatgccc
    17761 gcgcaggcgg tgcaggtcgc ggcggccggc aattccatcg cggtgctgcc cttcgacaac
    17821 cagagcagcg acaaggacca gcagtacttc tccgacggcc tgtccgaggg cttcatcatc
    17881 gcgctgtcgc gcatcgacgg gctgcgggtg atcaatcgca agtcctcgtt cgagttccgc
    17941 gacagcaagg acgacagcaa ggccatcgcg gccaagctgg gcgtttccca tttgctggag
    18001 ggcagtgtca gccgggtcgg cgacaccgtg cgcatccgcg ccagcctgac caaggccgcc
    18061 gatggcagca ccctgtgggc ggaaaactac gaccgcccct acaaggacct gttcgccctg
    18121 caggaccaga tcacctcgca ggtggccgcg gcgctgaagt cgcgcctgca gccggcatcg
    18181 gcccccaagg cccagagcga ccatcccccg ggcggcaacg tcgatgccta cgacgcctac
    18241 atgcagggac tttcggctag cagcatggaa catgcggtcg aggccttcga ccgtgcgacg
    18301 agcatcgatc ccgcctacgc cctggcgtat tccaggaagg cgcgcttgct gatcgacaac
    18361 atctcggccg gaaccctgtc cggcaagcgt gccgaagagg cctacgcgga ggcggacgcg
    18421 gccatcgcac aggcgatgcg gctcgccccc gaccaggcca cgacccggat cacgcgcggc
    18481 tacctgctca tgctgcgcga cttcgactgg cgcggtgccg aggccgagtt gaagcgcgcg
    18541 gtcgaattgg cccccgacga tggcgatgcg ttgtacatgc tgggcttggt gcgggccagc
    18601 cagggcgacc tggcgcaggc ggtcgagttg acccgcaagg cgttgcagct gaatccgcgc
    18661 agcgccgact ggtatcgctg gctggtggcc tacctgatgc cgctgggccg gctggacgaa
    18721 gccgagcagg cgatcgccaa ggccatcgaa ttgcagccgg agggcatcta caaccaccac
    18781 ctgctggcga tcgcgaaggt cctgcgcaag gatccggcgg cggcccgcaa ggcggccgag
    18841 gcggagccag cgggtccgtg gcgtgatttc gcccttgcgc ttgcggcgca agccggtccg
    18901 gatcgcgccg ccgcggatgc cagcctggag ctggtcgggc agtatgccga gggttgggcg
    18961 ttccagatgg cccaggtgta cgcggttcgc ggcgagcccg atgcgatgtt cgactggttg
    19021 cagcgggcgc tgaaggcacg cgatccgggc atgcagagcc tgctctacga cgcactgctg
    19081 ctgcgctacc gcgacgatcc gcgctatgcg gcgctggcga agcaggctgg tttgccctgg
    19141 ccgccgatcg cagcgaagcc agcacctgcc ccgccggcgc ctgccaagcc ctgaccctca
    19201 gggctcgatg atcaggcctt ggcgacgggg atcggcttga gtacgccgta gcgctccttg
    19261 gctggcttgc cggccagctt cgggaactcg atcttcggcg cgtcgatggc ggtatccggc
    19321 aggcgatcca gcaggtcgcg gatcagcgcc agccggccgc gcttctggtc gttgaaatcc
    19381 accagcgtcc acggtgcgtg gcgcgtgtgg gtggccttga gcatgtcctc gcgggcgcgg
    19441 gtgtagtcgc ggtagcgctt gcgcgcctcc aggtcgatcg gcgacagctt ccagcccttg
    19501 agcgggtcca ccaggcgttc ggcgaagcgc ttctcctgct gttcctggtc gcagcacagc
    19561 caatacttga acagcaggat gccgtcgtcg accagttgct gctcgaactg cggggtggca
    19621 tgcaggaacg cctcgacctg cgcatcgctg gcgaagccca tgaccttttc gacgcctgcg
    19681 cggttgtacc aactgcggtc gaacaggacg atctcgccgg cgcccggcag ttgcgccgcg
    19741 tagcgctgga aataccattg gccggcttcg cgatcggtcg gcttgggcag cgcgaccacg
    19801 tggcattggc ggggattgag gtgttcgcgg atcgcgttga tcgcgccgcc cttgccggcg
    19861 gtgtcgcggc cctcgaacac cacgaccacg cgctggccgg tgtgctggac ccagcgcgcc
    19921 atgttcgcca gttccgtctg cagcggctgc agcagggctt cgtagtcctt gcgcttgagc
    19981 ttgctcatcg cgtgctcttc ttcgccggtt tcatgctgcg cgccacttcc agcagggcgc
    20041 cctgctggcg gctgcccagg ctgcggtagg ccgccagcag gtcgtgttcg cccttggtgc
    20101 gcgccggatc cagcgtgctc gccagctccg acagcagtgc accagcgggg acaccgaacg
    20161 tgcgcgccag ggcatcaacc tggtccttgc cgggcagttt ctcgcccttg agccaggcat
    20221 tgaccgtgct cacgccggcg cgcgggatgg cggtggcgat gtcgacgacg gtcaggccgc
    20281 tggccttgct gagcagtcgc agggtggtat cgagggccat cgtcactcct tgagccgggg
    20341 cttcaaattg gcgaggttgc agggccgggt gcgtgcatcg agttgcgcgg cgatgatccg
    20401 gtcccagccg gtgcggcagg ccgacgtcga accgggcagg gcgaacagga aggtcgcgtt
    20461 ggccagcccg gcgaacgcac gcgactgcag ggtcgaggtg ccgatctcct cgaagctgag
    20521 cgcgcggaac agttcgccga agccgtccat gtgcttgtcg agcaagggca gcagcgcctc
    20581 cggggtggaa tcgcggccgg tgaagccggt gccaccggtg accaggatgc cgtccacctc
    20641 gccgtcggcg atccagcgcg acaccagcgc gcgcatcgcg tacttgtcgt cgcggacgat
    20701 ggcgcgctcg aacaggcgat ggccggcatc gcgcaaggca tcgctcaggt aatcgccgga
    20761 cgtgtcgtta tccgcgctgc gcgtgtcgga aacggtcaac acgcacagcg acagtggtct
    20821 gaaatcggcg gcggcagtca tgcgccgagt gtaggccgcg accgcgcgcg ctgcatcagt
    20881 ggtgcagctt cgagaacccc agcgaatacc actgggtggc gtcctcgttc cagccggcgg
    20941 cgcggtacag cgcgcgggcc gcggcgttgt cctggctggt ttccagcgag atcccgatcg
    21001 cgccgtcttc ccgcgcgaac gcggcggcgg cgtccagcag ggcccgcgcc acgcccctgc
    21061 ggcgcgcgcc ggcctccacg aacaggtcgt tgaggatcca ggtcttcgcc gtgcgcaccg
    21121 aggagaacat cggatacagc tggacgaagc cgaccgcgcc gccgccgcgc ctggccacca
    21181 gcaccaccga ctcgccgaag cgcaggcgct cgcgcagcca ctggcgcgca cgcgggaggt
    21241 cggtgggctg tccgtagaac tggcggtagc catcgaaaag caaggccagc gcgtcgaggt
    21301 cggccgggcc ggcgcgggag atcgtggtgg tatccatggt catggttcct gtgccagtcg
    21361 ggcgagggct tcggcctgct gttcctgctg caggcgcagg accagcgggt ccaggtcgcc
    21421 ttccaggatg ttcggcaggt cgtacagggt cagcccttcg acgcggtggt cggtgatccg
    21481 gccctgcggg tagctgtagg tgcggatgcg ctggctgcgg tcgccactgc cgacctgcag
    21541 cttgcggtcc gcggcagtcg cggcggtgcg tttctcgagc tcggcatccg ccagcatcgc
    21601 ctgcaggcgt ttcatcgcct tgtcgcggtt ggcgtgctgg ctgcgctcgg tctggttttc
    21661 gacgaccacg ccggtgggca ggtgggtgat gcggatcgcc gattcggtct tgttgacgtg
    21721 ctggccgccg gcaccggacg agcggaacgt gtcgatgcgc aggtcggccg ggttgatcac
    21781 gatgtcggtg tcgccgccgg gttcgatcgc gatgatcgcc accgtcgctg ccgaggtgtg
    21841 gatgcggccc tgcgattcgg tcgccggcac gcgctgcacg cggtgggtac cggattcgta
    21901 cttgagcttc gcgtaggcgc catcgccttc cacgcgcgcc accagctcct tgtagccgcc
    21961 gtgctcgccg ggactggccg attccacttc gacccgccag ccctgcgctt ccgcgtagcg
    22021 cgaatacatc cggaacaggt cgccggcgaa gatcgcggct tcgtcgccgc cggtgccggc
    22081 gcgcacctcc aggtacaggc cgccgctatc gcgcgcgtcc ttcgggacca gttgggccag
    22141 cagttcgccc tcgagctgct gcaggcgcgc ggtcgcggac gcgatctcgt cgtcggccag
    22201 tccgcgcagt tcgggatcgc cgcgcatcgc ctcggcggcg gccaggtcgg cgtgcgcctg
    22261 cgcctcggca gccagcgcat ccgcgaccgg ctgcagcgtg gcgaactcgc gcgacaggtt
    22321 gcggaaacgc gtctggtcgg cggcgatggc gggatcggcc agcaggcgtt ccacttcctc
    22381 gcgccgttcc accagcgcgt cgagtttgcg tcgcagggcg ggggtcatgc gtcggcgctg
    22441 tccgtgccgg tcgcggggaa cagcttttcc gcggcgcggg ccagctcgcc gtcgccgctc
    22501 agtgcggcat cgcgcagggc gacggtaggg gcgtgcagca ggcgattggt cagggtgtgg
    22561 gccagcaggt ccagcgcctg ttccggcggc atcccggcgg cgagttgcgc ccgcgccttg
    22621 gccagcgcct cggcacgcgc cgcgtcgcca tgcgcacgca ggcgcagcag cggttgctgg
    22681 cggccgctgg cctgccagtg ctcgacgaag cgcgcagtct gcagttcgat gatcgcttcg
    22741 gcctgctggg cggcctcgcg gcggctgcgg cggttgtcct cgatggctcg ttccaggtcg
    22801 tcgaccgtgt acaggaacac gtcgcgcagg ccggagacgt cgctgtcgat gtcgcgcggc
    22861 acggcgaggt ccagcagcag catcggccgg tgccggcgca gccgcagcgc gtgttcgacc
    22921 tgcgcgcgat gcaggatcgg cgtgcggctg gcggtggcgg agatgacgat gtcggcctcg
    22981 gccaggtgct tgtccagttc ggccagcggc agggccatgc cgccgtggcg gctggccagt
    23041 tcctgcgcgt gggcgagggt gcggttggcg accagcaggc gctgcgcctt ggcctccacc
    23101 aggtggcgcg cggccagctc gatggtctcg ccggcaccga tcagcagcac cgtggagtcg
    23161 ctgagccggg cgaaactctc ctgcgccagc cgcaccgcgg tggaggccac cgagaccggg
    23221 ttcgcgccga tgcgggtgtc ggtacgcgca cgcttggcgg tggcgaaggc ctgctggaac
    23281 agccggtcca gctggccacc cagggcaccg gcctggcgcg ccgccgacca cgcctgcttg
    23341 acctggccga ggatctgcgg ctcgcccagc accagcgaat ccaggccgct ggcgacccgg
    23401 aacaggtggc gcacggcgtc ggcatcgcga tgccggtaca ggtaggcctg caggtcgccg
    23461 ccggcctcgg gatgcgcgct cagccagccg gccagggcct ggccgtcatc gtccgaatgg
    23521 gcgtacagct cggtgcggtt gcaggtggag agcagcacgg cttcgcgcac ctggggcaac
    23581 gcgcgcagcg aggcgagcgc cggcgccagc gaatccgggg cgaacgccac cttctcgcgc
    23641 aggctgaccg gcgcggtctg gtggttgatg ccgaggacgt gcaaagccat gcgagaaatc
    23701 cgttcaggtg cctgcgataa gctgccggcc cgatccgtca ttgtaccggc ccgccccatg
    23761 cccatcccgc gacacgctgg tccacgcctg ctgctgttgg cggcactgct cgctcccggc
    23821 ctggcactcg ctgcctcgaa gccctctccg gcgaaggtcg tggtcgatcc gatggaagcc
    23881 agcctggccg gcgaattcgc cctccaggcc gggcaattgc ccgaggccgc ccgccagtac
    23941 ctgcaggcgg cgcgcagcgc cagcgacccg gtgctggccg aacgcgccac ccgtatcgcc
    24001 ctgctggcgg acgaggacgc gctggcccgt gacgcctacg cgacgtggcg atcgctcgcg
    24061 cccgagggcg gcgaagcgca acgcatggtc gcggccggtc tggccttgcg ggcgggcgac
    24121 aagcgtgcgt cccgccgcga gctgcgtacg ttgctcgccg agggcggcaa gggctggcgc
    24181 agcgcgctgg cggcgctgtt cggcgcggtc ggcaagcagc agaagctggt ggtggcgatg
    24241 attgacgggg tcgtcgacga caacctgctg cccgacgacc tccaggcctg gctcgggttc
    24301 ggcgggttgg cgcagcggct ggagcagccg gcgctggtgg atcgcatcgt cggcgagatc
    24361 gtccgccgct acccgggcga accgcgggtc gccctgctgc gcgcccaact cctgcgcgag
    24421 ggcggcaagc ttggcgaggc ccggaccctg ctcgccgggc tggaagacgc cgccaagctc
    24481 tcgccccagc tgcgctggtc ggtggccggc gaatacgatg cccttggcga cagcgccaag
    24541 gccgcgcagg tgttgggctg gggcccgcag gacgatgcca gccacgcgca gcgcgcgatg
    24601 ctgctggaca aggccaagga caaggccggg ctggccgccc tctacgagga actgaagcgc
    24661 gaggccaccc atcccaaccc gatgcgcagg ttgttgctgg gccagctggc ggaattgctg
    24721 gaacgctatg aggatgcccg cggctggtac gccaacgtcc ccggtgaagg cgtgcagggc
    24781 atcgcccgcc tgcgtggcgc caatgtgctg cacgcgatgg gtcgcaaacc tgcagccttc
    24841 gatgaactgc atgcgatcca ggccgaccag accctggatg aggaagcccg ccgcgacggt
    24901 tacctgctgg aagccgagtt gcggctcaag gacaaggacg aggctggcga gctggacgct
    24961 tatgcccgcg gcctggccgc gttcgccgat gacgaggcac tgttgtactc gcgcgccctg
    25021 ctgtgggagc gccgcgacga gatcgccaag gccgaggccg acttccggcg catcctggtg
    25081 atctcgccgg acgacgtgaa tacgctcaat gcgttgggct acacgctggc cgaccggacc
    25141 acccgctatc gcgaggcgct ggaactgatc gaccgtgccc gcgtggccgc gcccggcaac
    25201 gccgcgatca tcgacagcta tggctgggtc ctgttccgcc tgggcaagcc gcgccaggca
    25261 ctcgaccacc tgcgccacgc ctacgcgctg cagaaggatg ccgacatcgg cagccacctc
    25321 ggccacgtgc tgtgggtgct cggcgagcgt gatgaagcgc gcaagtattt cgaggaagca
    25381 cgcaagctcg atcccgagaa tcgctcgctg cagcgcgccc tgcaggccac cggggcgtga
    25441 accggcggat cgcgctggcg gcgctgggca tggccgtgct ggcgggctgc accggcgtgc
    25501 cggtcgcccg gccagccgcg cccggccagg tggtcggcac cgatgcggac tcgccgcaag
    25561 cgatggcgca gcgccagcgc atcgaacgac tgggcctggc cgatggcgat tgcagcgccc
    25621 ccgcatgggc gctgaccggc cgggtcgcgc tgtcgaacgg caaggatggc ggcagcggcc
    25681 gcatcacatg gtcgcaggcg gccggcgagg cgaggatcga actcaccgcg ccagtgacgc
    25741 gccagggctg ggcgctcgat gtcgatgcgg acggcgcgac cctgcagggc gttcccggcg
    25801 gcccggtgca tggcgcagat gccgccgccc tgctgcgcga gcgcacgggc tgggatatcc
    25861 ccgtcgccgg cctgggttgc tgggtgcgtg gtgcgtgggc cagcgaggcc gcgttcggcc
    25921 tggccacggt cggctacggc gaggatgggc gcgtgcagcg gatcgagcag gccggctgga
    25981 ccatcgacta cgccgactgg aagcccgatg ccgccagtgg agcggacctg cccgcgcgca
    26041 tcacggcctt gcgcaacacg gaccgggtgc gcctggtcgt ggatcgctgg gccgtggact
    26101 gagcggggac atggctgatt tccccgcgac cgaaggctgg agccgttggc cggcaccggc
    26161 caagctcaac ctgttcctgc gcatcaccgg tcgccggccg gatggctacc acgcgctgca
    26221 gaccgctttc cggctactgg actggggcga tgtgatccat ctgcggcgtc gtaccgatgg
    26281 tgtcatcgtc cgggtcgggg ctggcgcgga cggtgtcgcg gaagccgatg acctgatggt
    26341 gcgcgcggca aaacttctga gaaaagagac aaattgtcgc caaggtgctg atatcggcat
    26401 cgaaaagaac attccgacgg gtggcggctt cggcggtggt tcctcggatg cggcaaccgt
    26461 actggtcgtg ctcgaccggt tgtggaacct ggctctcggg gtcgaccggc tcgcggcgct
    26521 gggcttgcag ctgggtgcgg acgtgccggt gttcgtccgc ggccacaacg cttgggccga
    26581 aggcgtgggc gaagtgctca ccgcgatcga gctgccaccg gcttggtacg tggtcgccag
    26641 tcccggtgtc catgccgcca ccgccgccct gttccaagcc cctgatttga cgcgcaatgc
    26701 tgcacccgcg aaaataacgg gcttcctttc cggaacatcc ccgtcagggg ccgtgctcga
    26761 caatgcgttc gagccggtgc tgcgccgccg tgaacctgcc gtcgaggcct tgttcgcgca
    26821 gctggcgtcc atcggcacgc cacgcttgac gggttcgggc agcggttgtt tcgtcgaatt
    26881 cgcccagcgc gaatccgccg aggcggcgct cgcttcgctt ccgcccggca tccatgcctg
    26941 gctggcgcgg ggtgcggaga ggtcgccgct gctggatgca gtggaaggtt tcaatgcagg
    27001 ggcgtcgcca agcggttaag gcaccaggtt ttgatcctgg catgcgtagg ttcgaatcct
    27061 tccgcccctg ccaatcttgt tccacgggtt cgttcgcacg agctgtacct ccttcgccgc
    27121 gcagcgtgtc cgcgcggtcc attgcaccgc cacggtcaga gcgcagcgga accagccatg
    27181 caagacgatc gcaatctttt ggtgttttcc ggcaacgcga accggccgct ggccgatgcg
    27241 gtctgccgcg aactgggcgt gcgcccgggc aaggcgctgg tgagcacctt ctccgacggc
    27301 gaagtgcagg tcgagatcga ggagaacgtg cgccggcagg aggtcttcat catccagccg
    27361 accaacgccc cgaccgccga gaacttcatg gagttgctgg tgctggtgga cgcgctgaag
    27421 cgcgcgtcgg cggccagcgt gaccgcggtg gtgccgtact tcggctacgc ccgccaggat
    27481 cgccgcccgc gctcggcgcg cgtgccgatc acggccaagg tggccgcgaa gatgatccag
    27541 gccgtgggct
//
LOCUS       DAOOCX010000016        27452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1400_length_27452_cov_7.793189, whole genome shotgun sequence.
ACCESSION   DAOOCX010000016 DAOOCX010000000
VERSION     DAOOCX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 27452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27452
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1400_length_27452_cov_7.793189"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PK399_02775"
     CDS             complement(<1..643)
                     /locus_tag="PK399_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HPW12084.1"
                     /translation="MTPSPSPAPRELSLRDPALYANRELSQLDFNFRVLAQARDPAVP
                     LLERLRFLCISCTNLDEFFEIRGGSVRHAIEFGLPPAADGLAPSVLINRIHDRAAELV
                     EAQYRCFYEELRPALADEGIRLLQREQWTPEQTRWLRDHFRDEIMPVLSPLGLDPAHP
                     FPKILNKSLNVVVLLEGKDAFGREGHMAIVRAPRSLPRIIRLPDQDHQPGSGLP"
     gene            complement(640..1971)
                     /gene="phoR"
                     /locus_tag="PK399_02780"
     CDS             complement(640..1971)
                     /gene="phoR"
                     /locus_tag="PK399_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HPW12085.1"
                     /translation="MSPDARSAWFRTLGQLALVLVAGLGVGLLLGYPWPSLTLAALGV
                     VAWHYWKLRNVLLRLTARQRLAPSSGVGVWNELDRLLSRSQGEMRARKRRLLAMLRAY
                     RAAAAALPDAVVVVERNTQRVLWFNEAATPLLGLHYPRHQDAPLVAALQPLPMAQWLA
                     SGRNAEPMTDVASPANPDLRLHLRLIPYSDELWLLVARDVTKMMRLEHMRRDFVANVS
                     HELRTPLTVVHGYLDMLDPEDNPDWAPMLAEMQRQSQRMTQLVEDLLTLSRLEARDTL
                     PEDHVAMAPMLATLRREAEALSQGRHRISVDDDARIDLCGSTKELHSAFSNLVANAVR
                     YTPADGEIHVRFGRDPDGGATLSVRDTGYGIPAAHLPRLTERFYRVSTSRSRESGGTG
                     LGLAIAKHVLGLHDARLDIASEVGKGSTFACRFSPARVLPRDALPLPTTTP"
     gene            complement(2019..2720)
                     /gene="phoB"
                     /locus_tag="PK399_02785"
     CDS             complement(2019..2720)
                     /gene="phoB"
                     /locus_tag="PK399_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HPW12086.1"
                     /translation="MQKHILIVDDEPAIRDMVAYALRKGEYEPAHAGDAREAQAAIAE
                     RVPDLILLDWMLPGTSGLELARRWRKDALTRDIPIIMLTARGEENDRVGGLEAGVDDY
                     VVKPFSARELLARIRAVLRRSREDDEDGSVQVGGLRIDGAAHRVFADAAGGPIPVHIG
                     PTEYRLLHFFMTHPERVYSRSQLLDHVWGGSVYVEERTVDVHIRRLRKTLEPHRLDPM
                     VQTVRGAGYRFSAAA"
     gene            complement(2805..4460)
                     /locus_tag="PK399_02790"
     CDS             complement(2805..4460)
                     /locus_tag="PK399_02790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPW12087.1"
                     /translation="MTIARNDRFRRRMLPMALACGLAFTTGATVAQDTRLPDIGSSAG
                     NVLSPARQAEYGGMVLAQLRHEGYVLEDPLLDGWLRDVGQRLAGASDRPQQAFTFFLL
                     RDRQINAFATLGGYIGVNAGLILSAEREDEMAGVLAHEIAHVTQQHVLRGAEKAQRDS
                     LPILLAMLGAIVAAQQAGGNSSGDATSAAVMTGLGLLQQRQIDYTRDNEAEADRVGIR
                     NLSRSGYDPEAMADFFQTLQSVVRMNQGDERARAPSYLQTHPVTLTRVSEARERAAKL
                     EAPTVAATTTAGNNPLLPPGLSIATTTAGQRGQGATGDFGWARERLRVLSADTPAQAI
                     REYQQMRARQPLDDARRYGLAIAHQLAGQGQEALQELNPLAAAHPGNLWLQVAMAEAE
                     ARSGQLAQADRRLESLLARMPTHRALALSYARVLAERNTRDAGSRAVAVLRPLLATAS
                     ADPLFQQAYARANEIAGDDVRAGEAWAEAAFLGGRPEQALVQLNNLKKRATLDYYARS
                     RIEARIAAITPTVLELRRQGIKDEEATGRLSVGVRAGGTASDR"
     gene            4611..4859
                     /gene="grxC"
                     /locus_tag="PK399_02795"
     CDS             4611..4859
                     /gene="grxC"
                     /locus_tag="PK399_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HPW12088.1"
                     /translation="MYSSAICPYCVMAKNFLKSKGLAWTEVRIDTDPVAREAMMAKAR
                     RTSVPQIFVGDVHVGGYDDMMALHRAGKFEPLLAGEGA"
     gene            4856..5251
                     /locus_tag="PK399_02800"
     CDS             4856..5251
                     /locus_tag="PK399_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPW12089.1"
                     /translation="MSGGGSEEDRVKAFTEFRQRMNARILGEPNQVVRRFFALDTQTY
                     QAGALDVKTKELCGLVASMVLRCDDCISYHVAQCKEAGVNRDEMFEAFSIGLVVGGSI
                     VIPHLRRAVDFLDKLEQGEAASPDAHDHG"
     gene            5360..6367
                     /locus_tag="PK399_02805"
     CDS             5360..6367
                     /locus_tag="PK399_02805"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020852046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HPW12090.1"
                     /translation="MTTTITVIRGDGIGPEIMDATLHVLDAMQLGLQYEFADAGLAAL
                     EKHGELLPAATMDSIRRNRIALKSPLTTPVGEGFSSINVELRKRFDLYANVRPAKSFP
                     NTKSRFPSGVDLITVRENTEGAYSGEGQNVSADGETATLIQKITHRGSERIVRYAFEL
                     ARKTGRKKVTVVHKANILKSTSGLFLKTAREVAQLYPDIQCNEMIVDNTCMQLVMKPE
                     QFDIIVTTNLFGDIISDLCAGLVGGLGLAPGANIGTEAAIFEAVHGSAPDIAGKRIAN
                     PCALLLGAAQMLDHLSLPEQAERLRKAIIATLEAKDSLTPDLGGDGNTLSFAKAIAAR
                     L"
     gene            complement(6463..7230)
                     /locus_tag="PK399_02810"
     CDS             complement(6463..7230)
                     /locus_tag="PK399_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HPW12091.1"
                     /translation="MRSGNPALKDNTFLDLGSGTLVRNDAGAMSINGTVNKTALLLVI
                     TLAGALFTWSQFATAVAAGNPGAVMPYVWGGAIGGFVVALVTIFKKEWAMVTAPVYAV
                     LEGLFLGAVSAMFEMRYPGIVMQAVGLTFGTLAALLMAYRSGLIKATENFKLGVVAAT
                     GGIALLYLVNIGMRLFGFDGMGFIHESSLMGIAFSGFVVVIAALNLVLDFDFIESGVE
                     QGAPKHMEWYAAFGLLVTLIWLYLEMLRLLSKLQSRN"
     gene            complement(7327..7411)
                     /locus_tag="PK399_02815"
     tRNA            complement(7327..7411)
                     /locus_tag="PK399_02815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7375..7377),aa:Leu,seq:tag)
     gene            7576..7652
                     /locus_tag="PK399_02820"
     tRNA            7576..7652
                     /locus_tag="PK399_02820"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7610..7612,aa:Pro,seq:tgg)
     gene            7703..7779
                     /locus_tag="PK399_02825"
     tRNA            7703..7779
                     /locus_tag="PK399_02825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7737..7739,aa:Arg,seq:tct)
     gene            7815..7891
                     /locus_tag="PK399_02830"
     tRNA            7815..7891
                     /locus_tag="PK399_02830"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7849..7851,aa:His,seq:gtg)
     gene            7956..8031
                     /locus_tag="PK399_02835"
     tRNA            7956..8031
                     /locus_tag="PK399_02835"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7989..7991,aa:Lys,seq:ctt)
     gene            8234..9526
                     /gene="tig"
                     /locus_tag="PK399_02840"
     CDS             8234..9526
                     /gene="tig"
                     /locus_tag="PK399_02840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPW12092.1"
                     /translation="MQVSVESTGNLERRVILSLPAGDVDTQVGGRLREIARTARIKGF
                     RPGKVPTKVIEQRYGGQVRAEIVDGLLRQGMDQAVRENELRIAGAPQISQDVEAGEGE
                     LKYVASLELVPEFGDLDMTKLQVVRNTSEVTDADIERMLENLRQQRASWTKVVRAAKT
                     GDMANLETASTIDGARMPAEGFEKTSTVLGSGAMYPEVEAAIAGMQPGEEKTLDVTLP
                     ADWHMPQFAGKTVTSTFKLVDVAEQVLPEVDAAFIRSFGVKSGDLDQFKAEIRSNLER
                     ELKGALMNRLRREVGEQLIAAYADVELPPRLVENEARVLLANATEQARRQGQQVGAGI
                     EAFQDAGRKRVLVALLVGEVARRNDLRLEQARLNETMRLIASTYEQPEQVIALYRNDP
                     QLMQGLQNRVMEEQVIDWIAERAQHTEQAIGFQEAIGQ"
     gene            9551..10174
                     /gene="clpP"
                     /locus_tag="PK399_02845"
     CDS             9551..10174
                     /gene="clpP"
                     /locus_tag="PK399_02845"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPW12093.1"
                     /translation="MDPIKALNLVPMVVEQTSRGERAYDIYSRLLKERVIFLVGGIDD
                     HVANVVVAQMLFLEAENPEKDISLYINSPGGIVTAGMAIYDTMQYIKPDVSTICVGQA
                     CSMGAVLLAAGSSGKRYALPNSRVMIHQPLGGAQGQATDIDIQAREILSMRQRLNEIL
                     AHHTGQPIDTIARDTERDNFKSAEAAREYGLVDEVLVRRPDESIQAG"
     gene            10312..11604
                     /gene="clpX"
                     /locus_tag="PK399_02850"
     CDS             10312..11604
                     /gene="clpX"
                     /locus_tag="PK399_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPW12094.1"
                     /translation="MSEDRQGRTTGDSTKILYCSFCGKSQHEVRKLIAGPSVFICDEC
                     VELCNDIIREELEEKAQSTRSSLPKPREILEVLDQYVIGQRRAKKTLAVAVYNHYKRI
                     ESRQKSSDDVELAKSNILLVGPTGSGKTLLAETLARMLNVPFTMADATTLTEAGYVGE
                     DVENIIQKLLQKCDYDVEKAQHGIVYIDEIDKISRKSENPSITRDVSGEGVQQALLKL
                     IEGTVASVPPQGGRKHPQQEFLQVDTRNILFIVGGAFAGLDKVIQQRSSEAGGIGFGA
                     KVKSVERKADIGQVLAQVEPEDLIKYGLIPEFVGRLPVVATLEELDEPALMKILTEPK
                     NAITKQFRKLFEMEGVELEIRPDALAAIAKRALKRKTGARGLRTIVESVLLDTMYDLP
                     SLENVSKVVVDESVIEHKSEPYLIYQSAPAPAKAASAD"
     gene            11771..14203
                     /gene="lon"
                     /locus_tag="PK399_02855"
     CDS             11771..14203
                     /gene="lon"
                     /locus_tag="PK399_02855"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPW12095.1"
                     /translation="MSSPAEHPSVALPVLPLRDVVVFPHMVIPLFVGRDKSIRALDIA
                     MEGDKRILLVAQKSAETDDPGATDLHPIGTLAQVLQLLKLPDGTIKVLVEGLVRARVD
                     GVREEDGALSGNADVVDSAETREPREVEAIARSLMSMFEQYIKTNRKLPPELMQTLSG
                     IDDPSRLADTIAAHLGVRLSDKQKLLELLDTAERLEMLVGLVDGEIDVQQMEKRIRGR
                     VKSQMEKSQREYYLNEQMKAIQKELGDLDDAPNDLDEIARKIAAAGMPKPVEAKAKAE
                     FAKLKQMSPMSAEAGVVRNYLDWLLGVPWNARSKVRKDLKAAQDTLDADHYGLEKVKE
                     RILEYLAVQTRVAKMKGPILCLVGPPGVGKTSLGQSIAKATNRKFVRMALGGVRDEAE
                     IRGHRRTYVGSMPGRIVQNLNKVGTKNPLFVLDEIDKMSMDFRGDPSSALLEVLDPEQ
                     NHTFNDHYLEVDLDLSEVMFVATSNSLNIPGPLLDRMEVIRIPGYTEEEKINIAMRYL
                     VPKQLKANGLREGELKIAENAITDIVRYYTRESGVRNLEREIAKIARKVVKEIALKGP
                     QPKGSKPKTVSVTNKTLDKYLGVQRFDFGRVEAENEVGLVTGLAWTEVGGDLLQIEST
                     LVPGKGALLLTGQLGDVMKESASAALSVVRARTERLGIELDFLQKYDVHVHVPDGATP
                     KDGPSAGIAMATALVSMLTKIPVRHDVAMTGEITLRGRVSAIGGLKEKLLAALRGGIK
                     TVIIPDENRKDLVDLPAAVTRHMRIVPVKWIDEVLDLALERPLPGRGVPGEGTQPPLS
                     KPGERTSSDVKH"
     gene            14420..14692
                     /locus_tag="PK399_02860"
     CDS             14420..14692
                     /locus_tag="PK399_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPW12096.1"
                     /translation="MNKSEFVDAIADGAELTKADAGRALDAMVTAITKALKKGDTVTL
                     VGFGTFSVRKRGARTGRNPRTGETIKIKASKNPAFKAGKALKDAVN"
     gene            14786..14860
                     /locus_tag="PK399_02865"
     tRNA            14786..14860
                     /locus_tag="PK399_02865"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14818..14820,aa:Val,seq:tac)
     gene            14910..14986
                     /locus_tag="PK399_02870"
     tRNA            14910..14986
                     /locus_tag="PK399_02870"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14944..14946,aa:Asp,seq:gtc)
     gene            15117..17084
                     /locus_tag="PK399_02875"
     CDS             15117..17084
                     /locus_tag="PK399_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPW12097.1"
                     /translation="MLQTLRDKTSGWIATVILGLLIIPFAFFGMESYLSQKVDTYAAR
                     ITQAPSWWPSAPQAWPVSYLWKTHDIEAQDYRQRLETLRMRQRDEQGDKFDGKAFESA
                     ENKRQVLDDMIDEQLMRLAAESDGIVISDAEIRKEIEQIPDFQVDGKFDADRYQLLLA
                     SQNPPQSPRTFEQKVRDGLQYGLIPSRLARSGFVTDVELDRLMRLLGERRDVSFVVMP
                     PAPVDNAPVTPAQIQAWYAAHAREYRTPETVRLEYVEVDASTLPVPLADEAALRKLYD
                     EQAAKYSTAEQRSVSHILVQVAADASDADKKAAEARAKKIAEQARAPGADFAALARAN
                     SDDAGSKAAGGSLGWLTKGGMPGPFDEAAFAMRAGEVRGPVKTDFGWHVIKVDELRGG
                     TRQPFEAVRAQLEQELVEGGRERAFNELSGKLVDAIYKNPNSLEPAAKSLGLAVQTTP
                     AFSRNGGPGIAGDAKVLRAAFSETLLRDGTASDPIELSPTRTVLIRVIEHKPEAALPL
                     SQVGNAVVMAIRADRQRKAAEAAADALVKAAKASGLPAAATAATLPLGEANDVRRGSP
                     VPSREAVDAFFRVPRPQDNLIPVGKTEADGQFIVFAIRAVRDGDIGQVTAAERDQVRQ
                     QLSQASGLQAQKAFVRATRAKYQIKVAEDRL"
     gene            complement(17190..17978)
                     /locus_tag="PK399_02880"
     CDS             complement(17190..17978)
                     /locus_tag="PK399_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HPW12098.1"
                     /translation="MGFLQGKRALVTGIASQRSIATGIADAMHREGAELALTYQNEKL
                     KSRVEDAAANYGSKIVLPLDVADDGQIDACFDELGKHWADGFDILVHCIAFAPREAIE
                     GDFLDGINRERFHIAHDISAYSLAAIGKAARPLMKGRNGSVLTLSYLGAERALANYNT
                     MGVAKASLEATVRYMAMALGPEGTRVNAISAGPIRTLAASGVANFRKMLTQFEAVAPL
                     RRVVTIEDVGNTAAFLCSDLAAGITGEITYVDAGYNIMGMSGIE"
     gene            complement(18022..18864)
                     /locus_tag="PK399_02885"
     CDS             complement(18022..18864)
                     /locus_tag="PK399_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPW12099.1"
                     /translation="MKAARALALALLLASGLGLASSAPHSRSGGEILQRFHAGLADAD
                     CRKSSARWRSHYARAAEHLADEDEIALAMFGYVLDEVREAGLPSEFALIPFVESRYRA
                     DARSRGGPAGLWQFTAGTARRHGMTVRGAVDERFSVVASTEAAVRYLARLHRLFGRDW
                     RQAVMAYNAGEGALRASRRKGAARMSGITRSYPAKLNAIACLFDQRADNARWRRAIER
                     QVPRLAPRRLPAGSRDLREWAVAQGLDADLVLALNPGWHPGARAVLAPIGADAGRTAP
                     DEAN"
     gene            complement(18861..19640)
                     /gene="gloB"
                     /locus_tag="PK399_02890"
     CDS             complement(18861..19640)
                     /gene="gloB"
                     /locus_tag="PK399_02890"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HPW12100.1"
                     /translation="MPLQQVPAFEDNIVWLLRDAAGGSLVVDPGQASPVLDALAGAPP
                     PHAILLTHHHNDHIGGVPELLRNWPGVPVIAPRDERIGFATRRVGDGDLAEAGPWRFT
                     VIDIPGHTRSHIAFHGYDLDGTGLLFCGDTLFSLGCGRMFEGTPAQMHASLQRLAALP
                     AGTRVCCGHEYTLANAAFAIAVDPGNDALRKRADTVRALRAAGRSSLPSTLEDERARN
                     PFLRCNDPAVHAAAETHAGHPLASPPEVFATLRRWKDGFRG"
     gene            19927..20133
                     /locus_tag="PK399_02895"
                     /pseudo
     CDS             19927..20133
                     /locus_tag="PK399_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20361..20816
                     /gene="rnhA"
                     /locus_tag="PK399_02900"
     CDS             20361..20816
                     /gene="rnhA"
                     /locus_tag="PK399_02900"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HPW12101.1"
                     /translation="MTARKRIEVHTDGACLGNPGPGGWAALLRWREVERELSGGEADT
                     TNNRMELMAAIAALEALREPCDVILTTDSQYVRQGILDWLANWIRRGWKTSGGDPVKN
                     RDLWERLQAASAPHKIDWRWVKGHSGHPENERVDVLARTAAEQIRDKAG"
     gene            20816..21529
                     /gene="dnaQ"
                     /locus_tag="PK399_02905"
     CDS             20816..21529
                     /gene="dnaQ"
                     /locus_tag="PK399_02905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HPW12102.1"
                     /translation="MRQVVLDTETTGLEWQKGNRVVEIGCVELVERRPTGRTFHAYLN
                     PDRDMEPGAQEVTGLTREFLADKPRFAEVADEFLAFIDGAELVIHNAAFDIGFLDAEL
                     ARLEGVGKIRDRCAVEDTLLMARQRFPGQRNSLDALCKRLGVDNSQRQWHGALLDAQI
                     LLDVYIALTSGQSEIGFASEAAPRAAETVQVVARSSDPSLRPRVQVTTEDAAAHAARL
                     AGLQKKAGRCLWLEIEQAG"
     gene            complement(21542..22246)
                     /locus_tag="PK399_02910"
     CDS             complement(21542..22246)
                     /locus_tag="PK399_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPW12103.1"
                     /translation="MIEFGHLTHVGLRRELNEDTYYGDSELGLWLVADGMGGHEYGEV
                     ASALAREAIVREVRAGNTLAEAIRTADEEIIRCSKRRGDSLPMGTTVVAARVNGNRFE
                     VAWVGDSRVYLWRDGALTQISQDHSYVQELIAQGAITADQARSHPHRNVVTQALGVTD
                     PVQLNVETLGGELRPGMQLLLCSDGLTEEVDDRSIARVLAHQECSAQECVDGLVAAAL
                     DGGGSDNVTVVLVRSH"
     gene            complement(22527..22616)
                     /locus_tag="PK399_02915"
     tRNA            complement(22527..22616)
                     /locus_tag="PK399_02915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22580..22582),aa:Ser,seq:cga)
     gene            22711..23118
                     /locus_tag="PK399_02920"
     CDS             22711..23118
                     /locus_tag="PK399_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HPW12104.1"
                     /translation="MSEILVPVSFGELLDKIAILQIKSERMSDPAKLANVRNELSALD
                     TTWDAHPASKQDIDALRADLKAVNERLWVIEDDIRLQEKAQAFDAEFIRLARAVYFEN
                     DIRARIKKDINLALGSSYVEEKSYQDYGTGQGA"
     gene            complement(23119..24171)
                     /locus_tag="PK399_02925"
     CDS             complement(23119..24171)
                     /locus_tag="PK399_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPW12105.1"
                     /translation="MPNPAAPRSLCLLRLSALGDVTHVLPLLHTLRSAWPDVAITWVI
                     GKGEHRLLAGLPGVRFVEYDKKTGLAGMRALRRELGQRFDVLLQLQVSARANLLSAFI
                     PARLRVGYDRSRSKEGHGLFINARIPNRPGIHVLDAIGSFCEPLGLVRDGVLWNLPVP
                     DDAHAWARAQWEDDGRPVLMVSPCSSHVLRNWRAERHAALADHAVSAGWRVVLCGGRS
                     DLERTTGNAILAAMTSADGVLDLIGKDTLKQLPALLARADLLVTPDSGPMHIANAMGT
                     KVLGLHAATNPHRSGPYSDRRFCVDRYDAAARTYRGKPASELKWGTRIEAEGVMDLVT
                     VEDAVAAFERYRAGIG"
     gene            24192..24935
                     /locus_tag="PK399_02930"
     CDS             24192..24935
                     /locus_tag="PK399_02930"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019305198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HPW12106.1"
                     /translation="MTGFDANEHLTPFRDARGYGAILFDRTQLRQPDPAWFSPQHWGE
                     AAQPVAEGGRGGAWFIQTGEGDAVLRQYLRGGWAASLSRDGHLWRGINRVRSFAEFRL
                     LRALREKKLPVPMPFAAYYRREGLHYRASILMQRLPGVRSLAALAAGDEPPWEQAGQL
                     VARFHRAGLDHADLNAHNILFDADGRGWMIDFDRSRLRIPATAWRQANLQRLLRSLHK
                     RHGSRSLVEVDAGFARLRAAYDAAWQRGY"
     gene            24949..25950
                     /gene="moaA"
                     /locus_tag="PK399_02935"
     CDS             24949..25950
                     /gene="moaA"
                     /locus_tag="PK399_02935"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HPW12107.1"
                     /translation="MSPPRDALARPLRDLRLSVIDACNFRCPYCMPADRIADDHGLDA
                     ASRLSFHEIETLVRGFARLGVRKLRLTGGEPLLRKRLPELVARLAVIDGIEDLALTTN
                     GSLLAAHARALREAGLTRITVSLDTLDPDAFRALSGGRGELRDVLAGIEAATAAGFGP
                     IKLNCVVQRGVNEGGIETLASFAREHHHVLRFIEYMDVGTCNGWRRERVVPSAELRDR
                     IAAHWPLRALDANYRGEVAERYAYDDGRGEVGFVSSVTAPFCGDCHRARVSADGMLYT
                     CLFAGQGHALRGVLARGEDALAARVADLWSKRADRYSEVRSEASTSRKHVEMYLVGG"
     gene            25969..26460
                     /gene="moaC"
                     /locus_tag="PK399_02940"
     CDS             25969..26460
                     /gene="moaC"
                     /locus_tag="PK399_02940"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPW12108.1"
                     /translation="MTKKRATLTHLDPTGRPAMVDVSAKPVTARQAQAGCRVTFPPAI
                     AAQLRDNGLKSAKGGIVDTAIIAGTMAVKKTHELIPFCHPLPIDGCRFSIDWHDARTL
                     RIECTVGTTHRTGVEMEALTGATVAALTVYDMCKALSHAIVIGPAKLLGKRGGKRDIG
                     QLA"
     gene            26457..26702
                     /locus_tag="PK399_02945"
     CDS             26457..26702
                     /locus_tag="PK399_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011257909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPW12109.1"
                     /translation="MSTRVEVLYFAALRDAAGIASEHVDTGAADLAGLYAEARVRHPL
                     PFAQTQLRVAVDGAFAGWGDAVRAGSTVAFIPPVSGG"
     gene            26704..27123
                     /locus_tag="PK399_02950"
     CDS             26704..27123
                     /locus_tag="PK399_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HPW12110.1"
                     /translation="MAHFRLSDAPIDAVALQSGLRDPRAGACASFEGWVRDHNDGRAV
                     DGLRYEAYVSLAEAEGAAILAEALERFDIVQATCVHRVGELAIGDLAVWVGASAAHRG
                     PAFEACRWIIDEVKARVPIWKHERYAGGDAGWLHPGP"
     gene            27207..27299
                     /locus_tag="PK399_02955"
     tRNA            27207..27299
                     /locus_tag="PK399_02955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27241..27243,aa:Ser,seq:gga)
BASE COUNT         4580 a         9002 c         9552 g         4318 t
ORIGIN      
        1 ctggcagccc gcttccaggc tgatggtcct ggtcgggcag gcggatgatc cgcggcagcg
       61 agcgcggcgc acgcacgatc gccatgtggc cctcgcgccc gaacgcatcc ttgccttcca
      121 gcagcaccac cacgttcagc gacttgttga ggatcttcgg gaacgggtgt gcgggatcca
      181 ggcccagcgg cgacagcacc ggcatgatct cgtcgcggaa atggtcgcgc agccagcggg
      241 tctgttccgg cgtccactgt tcgcgctgca gcaggcggat gccttcatcg gccagcgccg
      301 ggcgcagttc ctcgtagaag cagcggtact gcgcctccac cagttcggcg gcgcggtcgt
      361 ggatcctgtt gatcagcacg ctcggcgcca gcccgtcggc ggcgggcggc aggccgaact
      421 cgatcgcgtg gcgcaccgag ccgccgcgga tctcgaagaa ctcgtccagg ttggtgcaac
      481 tgatgcacag gaagcgcagc cgctccagca acggcaccgc cggatcccgg gcctgcgcaa
      541 gcacgcggaa attgaagtcc agttgcgaca gttcgcggtt ggcatacagc gcggggtcgc
      601 gcagcgagag ttcgcgcggg gcgggcgatg ggctgggggt catggcgtgg tcgtgggaag
      661 cggaagggcg tcgcgcggaa gcacgcgggc agggctgaag cggcaggcga aggtactgcc
      721 cttgccgact tcgctggcga tgtcgaggcg ggcgtcgtgc aggcccagca cgtgcttggc
      781 gatggccagg cccaggccgg tgccgccgga ttcgcgcgag cggctggtgg agacgcggta
      841 gaagcgctcg gtgagccgcg gcaggtgggc ggcggggatg ccgtaaccgg tgtcgcggac
      901 cgacagcgtg gcgccgccat cgggatcgcg gccgaagcgc acgtggatct cgccgtcggc
      961 aggcgtgtag cgcaccgcgt tggcgaccag gttggagaag gcgctgtgca gttccttggt
     1021 cgagccgcac aggtcgatgc gcgcgtcatc gtccacgctg atccggtggc ggccctggct
     1081 gagcgcctcg gcctcgcggc gcagggtggc cagcatcggc gccatcgcca cgtggtcctc
     1141 cggcagggtg tcgcgggcct cgaggcgcga cagcgtgagc aggtcctcga ccagttgggt
     1201 catccgctgc gactgccgtt gcatttccgc cagcatcggc gcccagtccg gattgtcttc
     1261 cggatccagc atgtccaggt agccgtggac cacggtcagc ggcgtgcgca attcatgcga
     1321 gacattcgcc acgaagtccc ggcgcatgtg ctccagccgc atcatcttgg tcacgtcgcg
     1381 cgcgaccagc agccacagct catcggagta gggaatcagc cgaaggtgca ggcgcaggtc
     1441 cggattggcg ggcgacgcga catcggtcat cggctcggcg ttgcgcccgc tggccagcca
     1501 ttgcgccatc ggcaagggct gcagcgcggc caccagcggt gcgtcctggt ggcgcgggta
     1561 gtgcaggccg agcaggggcg tggcggcctc gttgaaccag agcacgcgct gggtattgcg
     1621 ttcgaccacc accaccgcat ccggcagcgc ggcggcggcg gcgcggtagg cgcgcagcat
     1681 cgccagcagc cggcgcttgc gcgcgcgcat ctcgccctgg ctgcgcgaga gcaggcggtc
     1741 gagttcgttc cagacgccga cgccggacga cggcgcgagc cgctgccggg cagtcagccg
     1801 cagcagcacg ttgcgcagtt tccagtagtg ccaggcgacc acgccgagcg cggcgagggt
     1861 cagtgacggc cacgggtacc ccagcagcag gccgacgccc aagcccgcca ccagcaccag
     1921 cgcgagctgg ccgagcgtgc ggaaccaggc ggaacgggca tcgggcgaca tgcagggagt
     1981 ctaggcggtg tgcgcgtggg gcgtcgcccg ctcgcaggtc atgccgccgc ggagaaccgg
     2041 taccccgcgc cgcgcacggt ctgcaccatc ggatccagcc ggtgcggctc cagggtcttg
     2101 cgcaggcggc ggatgtgcac atcgaccgtg cgctcctcca cgtacacgct gccgccccag
     2161 acgtgatcga gcagctgcga gcggctgtac acgcgctcgg ggtgggtcat gaagaaatgc
     2221 agcaggcggt attcggtggg gccgatgtgg accgggatcg ggccgccggc ggcgtcggcg
     2281 aagacgcggt gggcggcacc gtcgatgcgc agcccgccga cctgcacgct gccgtcctcg
     2341 tcgtcctcgc gcgagcggcg caggaccgca cggatgcgtg cgagcagttc gcgggcggag
     2401 aacggcttga ccacatagtc gtcgacgccg gcctcgagcc cgccgacgcg gtcgttctcc
     2461 tcgccgcgcg cggtgagcat gatgatgggg atgtcgcggg tcagggcgtc cttgcgccag
     2521 cgccgggcca gttccaggcc gctggtgccg ggcagcatcc agtccagcag gatcaggtcg
     2581 ggcacccgtt cggcgatcgc agcctgcgcc tcgcgggcgt cgccggcgtg ggccggttcg
     2641 tactcgccct tgcgcagggc ataggcgacc atgtcgcgga tcgcgggttc gtcgtcgacg
     2701 atcaggatgt gcttctgcat gccggatcct gcgtggcggg aaaggccgcg tcctgcggcg
     2761 atcctgccat tacacgacgg tttggtgacg gtttggtgac accgtcagcg atcgctggcg
     2821 gtgccgccgg cgcgcacgcc cacgctgagg cggccggtgg cttcttcgtc cttgatgccc
     2881 tggcgacgca gttccagcac ggtcggggtg atcgcggcga tccgcgcctc gatccggctg
     2941 cgcgcgtagt agtccagggt ggcgcgcttc ttcaggttgt tgagctggac cagcgcctgc
     3001 tccgggcgtc cacccaggaa ggccgcctcg gcccaggctt cgccggcgcg gacgtcgtcg
     3061 ccggcgattt cgttggcgcg ggcgtaggcc tgctggaaca gcgggtcggc gctggcggtg
     3121 gccagcagcg gacgcaagac cgcgaccgcc cggctgccgg cgtcgcgggt gttgcgctcg
     3181 gccagcacgc gcgcatagct gagcgccagt gcgcggtggg tcggcatccg tgccagcaac
     3241 gattcgaggc ggcggtcggc ctgggccagc tggccgctgc gcgcctcggc ttcggccatc
     3301 gccacctgca accacaggtt gccgggatgg gcggcggcca gcgggttgag ttcctgcagg
     3361 gcttcctggc cctggccggc cagctggtgg gcgatcgcca ggccatagcg gcgggcgtca
     3421 tccagcggct gcctggcgcg catctgctgg tattcgcgga tcgcctgcgc aggcgtgtcg
     3481 gcgctgagca cgcgcaaccg ctcgcgtgcc cagccaaagt cgccggtggc gccctggccg
     3541 cgctgcccgg cggtggtggt ggcgatgctg aggcccggcg gcagcagggg gttgttcccc
     3601 gcggtcgtgg tggcggcgac ggtcggtgcc tccagcttgg cggcacgctc gcgggcttcg
     3661 ctcacccggg tcagggtgac cgggtgggtc tgcaggtagc ttggcgcccg ggcgcgctcg
     3721 tcgccctggt tcatccgcac gaccgattgc agggtctgga agaagtcggc catcgcctcg
     3781 gggtcgtagc cgctgcgcga caggttgcgg atgccgacgc ggtcggcttc ggcttcgttg
     3841 tcgcgggtgt agtcgatctg gcgctgttgc agcaggccca gcccggtcat caccgccgcc
     3901 gacgtggcgt cgccgctgga attgccgccc gcctgctgcg ccgccacgat cgcgcccagc
     3961 atggccagca ggatcggcag gctgtcgcgc tgggccttct cggcgccgcg caggacgtgc
     4021 tgctgggtga cgtgggcgat ttcgtgggcc agcacgccgg ccatttcgtc ctcgcgttcg
     4081 gcggacagga tcaggccggc gttcaccccg atgtagccgc cgagcgtcgc aaaggcattg
     4141 atctggcggt cgcgcagcag gaagaaggtg aacgcttgtt gcggacggtc gctggcgccg
     4201 gccaggcgct ggcccacgtc gcgcagccag ccatccagca agggatcctc cagcacgtag
     4261 ccctcgtggc gcagctgggc cagcaccatc ccgccgtatt cggcctgcct ggcaggcgac
     4321 agcacgttgc cggcggacga gccgatgtcg ggcaggcggg tgtcctgggc aaccgtggcg
     4381 cccgtggtga aggccaggcc acatgccaac gccatgggca gcatgcggcg ccggaaacga
     4441 tcgttgcggg caattgtcat ggtggcagga tgctgcgttc gcgggcgatg ggtgtgaacg
     4501 gggcttgagc aactggaaaa tacgcgctgc tgcccgcaga tacgacaaga ttgcagcacc
     4561 cgcgttcccg gagattgaac ttgagcaacg ccaccccccc cgtgatcacc atgtactcct
     4621 cggccatctg cccgtactgc gtgatggcca agaacttcct gaagagcaag ggcctggcct
     4681 ggaccgaggt gcggatcgac accgatcccg tcgcccgcga agcgatgatg gcgaaggcgc
     4741 gccgcaccag cgtgccgcag atcttcgtcg gcgatgtcca cgtcggcggc tacgacgaca
     4801 tgatggcgct gcatcgcgcg ggcaagttcg agccgctgct tgccggggaa ggcgcatgag
     4861 cggcggaggc agcgaggaag atcgcgtcaa ggccttcacc gagttccgcc agcgcatgaa
     4921 cgcgcgcatc ctgggcgaac cgaaccaggt cgtgcgccgc ttcttcgcgc tggacacgca
     4981 gacctaccag gccggcgcgc tggacgtgaa gaccaaggaa ctgtgcgggc tggtggcctc
     5041 gatggtgctg cgctgcgacg actgcatcag ctaccacgtc gcccagtgca aggaagccgg
     5101 cgtgaaccgc gacgagatgt tcgaggcgtt ctcgatcggc ctggtggtgg gcggctccat
     5161 cgtcatcccg cacctgcgcc gcgcggtgga tttcctcgac aagctggaac agggcgaggc
     5221 tgcgagcccc gacgcgcacg accacggctg accaaggtcg tagggccggc cacgggcccg
     5281 tctggcataa tgcgcggctc gaaatcccat gcgctgcgcg tcacgcgcgg cgcccgcctg
     5341 tctggaaccc cctgaactta tgaccacgac gatcaccgtc atccgcggcg atggcatcgg
     5401 cccggaaatc atggatgcca ccctgcacgt gctcgatgcg atgcaactgg gcctgcagta
     5461 cgaattcgcc gatgccggct tggctgcgct ggagaagcat ggcgagctgc tgccggcggc
     5521 gaccatggac tccatccgca ggaatcgcat cgcgttgaag tcgccgctga ccacgccggt
     5581 cggcgagggc ttcagctcga tcaacgtcga attgcgcaag cgcttcgacc tgtacgccaa
     5641 cgtacgcccg gccaagagct tcccgaacac caagtcgcgc ttcccgtccg gcgtcgacct
     5701 gatcaccgtc cgcgagaaca ccgagggcgc gtattcgggc gaaggccaga acgtctcggc
     5761 cgatggcgag accgcgaccc tgatccagaa gatcacccat cgtggctccg agcgcatcgt
     5821 gcgctacgcg ttcgagctgg cgcgcaagac cgggcgcaag aaggtcaccg tggtgcacaa
     5881 ggcgaacatc ctgaagtcga cttcgggcct gttcctgaag accgcccgcg aagttgcgca
     5941 gttgtacccg gacatccagt gcaacgagat gatcgtggac aacacctgca tgcagttggt
     6001 gatgaagccg gaacagttcg acatcatcgt caccaccaac ctcttcggcg acatcatctc
     6061 cgacctgtgc gcgggcctgg tcggcggcct cggccttgcg ccgggcgcca acatcggcac
     6121 cgaagcggcg atcttcgaag cggtgcatgg ctccgcgccg gacatcgccg gcaagcgcat
     6181 cgccaatcct tgcgcattgc tgttgggcgc cgcgcagatg ctcgaccatc tctcgctccc
     6241 cgagcaggcc gagcgcctgc gcaaggcgat catcgccacg ctggaggcca aggattcgct
     6301 gaccccggac ctgggcggcg atggcaacac gctgtcgttc gcgaaggcga ttgccgcgcg
     6361 gttgtagtcg cagtcgcgcg cgctggcgtt cacgatcgcc ggcaggatgg acatgaaaga
     6421 gggcgccgat cggcgccctt tttcgtcagc ctgcgatcag gttcagttgc gcgactgcag
     6481 cttgctcaac agccgcagca tttcgaggta cagccagatc agcgtgacca gcaggccgaa
     6541 cgcggcgtac cactccatgt gcttgggagc cccctgttcg acgccactct cgatgaaatc
     6601 gaaatccagc accaggttga gcgccgcgat caccaccacg aagccggaga aggcgatgcc
     6661 catcaggctg ctttcgtgga tgaagcccat gccatcgaag ccgaacaggc gcatgccgat
     6721 gttgaccagg tagagcagcg cgatgccgcc ggtggcggcg accacgccca gcttgaagtt
     6781 ctcggtggcc ttgatcaggc cgctgcgata cgccatcaac aaggccgcca gggtgccgaa
     6841 ggtcaggccg accgcctgca tcacgatgcc ggggtagcgc atctcgaaca tcgccgacac
     6901 cgcgcccagg aacaggcctt ccagcaccgc gtacaccggg gcggtgacca tcgcccactc
     6961 tttcttgaag atcgtgacca gcgccaccac gaagccgccg atcgcgccac cccagacgta
     7021 gggcatcacc gcgccgggat tccctgcggc cacggcggtg gcgaattgcg accaggtgaa
     7081 cagggcgccg gccagcgtga tcaccagcaa cagggcggtc ttgttgaccg tgccgttgat
     7141 gctcatggca cccgcgtcgt tgcgcaccag ggtgccactg ccgaggtcga ggaaggtgtt
     7201 gtccttcagg gctggattgc cgctgcgcat catgtcgtca tgtcccgtgt gagcgcggct
     7261 ctcgcgccgc ttgcgccgag cataacgcgg ccggcccggg tttgccgccg gccggcggag
     7321 ccgccgtggt gcgaaaggcg ggactcgaac ccgcacgcct tgcggcactg gaacctaaat
     7381 ccagggcgtc taccaattcc gccactttcg cgccggcgca ttgtagcggc gggcggcacc
     7441 gtcccgccgc cacgatgctg caaaccgcga gccgtgcgtt gacactgcgt ccccatggtc
     7501 ggacaatgcg cggctctttc gtcgcgctgg cgcgcagcgg aagtctgcag aaggcagcgg
     7561 ttcgggttcc gtcggcgggg tatagcgcag tctggtagcg cgcctgcttt gggagcagga
     7621 tgtcgggggt tcgaatccct ctaccccgac cattccgacg cggcattctg gcagtggagt
     7681 tcgatgcgac aatccggcac gggcgcccgt agctcaaccg gatagagcac cggccttcta
     7741 agccggcggt tgcaggttcg attcctgccg ggcgcgccaa cgtggtggat gttcgttcca
     7801 agcatgcaac tttggtgggt gtagctcagc tggttagagc accggattgt gattccgggg
     7861 gtcgtgggtt caaatcccat catccacccc aatgttgaag tagcgatgcg acgcggttgc
     7921 gttggcatcc ctgcaaacgc tacaatttct gttcggggcc gttagctcag ttggtagagc
     7981 agttgactct taatcaatag gtccaaggtt cgaatccttg acggcccacc aagtcccacg
     8041 aacgcccggc ttgccgggcg ttcgcttttc cgggcgtcga cgccctcctg ctgccacgat
     8101 acgcccgaaa cgggtggcgg gatgccagcg gatcggccaa actagcgcgt tccgccgggc
     8161 ggcttgcccg gcatccgtcc aaccccttac gcgcaaccgg gttcgcgcga acccgtgcag
     8221 gagtcatcgc atcatgcaag tctcggtcga atccaccggc aacctcgagc gccgcgtcat
     8281 cctcagcctg cccgcgggcg atgtggacac ccaggtcggc ggccgcctgc gcgagatcgc
     8341 gcgcaccgcc cgcatcaagg gcttccgccc cggcaaggtg ccgaccaagg tgatcgagca
     8401 gcgctacggt ggccaggtcc gtgccgagat cgtcgacggc ctgctgcgcc agggcatgga
     8461 ccaggccgtg cgcgagaacg agctgcgcat cgccggcgcg ccgcagatca gccaggacgt
     8521 cgaggccggt gagggcgaac tcaagtacgt ggcctcgctg gagctggtgc cggaattcgg
     8581 cgacctcgac atgaccaagc tccaggtcgt ccgcaacacc tcggaagtca ccgatgccga
     8641 catcgagcgc atgctcgaga acctgcgcca gcagcgcgcc agctggacca aggtcgtgcg
     8701 ggccgccaag accggcgaca tggccaacct ggagaccgcc agcaccatcg atggcgcgcg
     8761 catgccggcc gaaggcttcg agaagaccag cacggtgctg ggctccggtg cgatgtatcc
     8821 ggaagtcgag gccgcgatcg ccggcatgca gccaggcgag gagaagaccc tcgacgtgac
     8881 cctgccggcc gactggcaca tgccgcagtt cgcgggcaag acggtcactt ccacgttcaa
     8941 gctggtcgat gtggccgagc aggtgctgcc cgaggtcgat gccgcgttca tccgcagctt
     9001 cggcgtgaag agcggcgacc tcgaccagtt caaggccgag atccgcagca acctggagcg
     9061 cgagctcaag ggcgcgctca tgaaccgcct gcgccgcgaa gtcggcgagc agctgatcgc
     9121 cgcctacgcc gacgtcgaac tcccgccgcg cctggtcgag aacgaagccc gcgtgctgct
     9181 ggccaacgcc accgagcagg cccgccgcca gggccagcag gtcggtgccg gcatcgaggc
     9241 cttccaggac gcgggtcgca agcgcgtcct ggtcgcgctg ctggtcggcg aagtcgcccg
     9301 ccgcaacgac ctgcgcctcg agcaggcgcg cctgaacgaa accatgcgtt tgatcgcctc
     9361 gacctacgaa cagccggagc aggtcattgc gttgtaccgc aacgaccccc agttgatgca
     9421 gggcctgcag aaccgcgtca tggaagagca ggtgatcgac tggatcgccg agcgcgccca
     9481 acacaccgaa caggcgatcg gcttccagga agcgatcggc cagtaagcag accataacca
     9541 gcgagagacc atggacccga tcaaagccct caacctcgtg ccgatggtgg tcgaacagac
     9601 cagccgcggc gagcgcgcgt acgacatcta ttcgcgcctg ctgaaggagc gggtgatctt
     9661 cctggtcggc gggatcgacg accacgtcgc caacgtggtc gtcgcgcaga tgctgttcct
     9721 cgaggccgag aatcccgaga aggacatcag cctgtacatc aactcgccgg gtggcatcgt
     9781 caccgcgggc atggcgatct acgacaccat gcagtacatc aagccggacg tgagcaccat
     9841 ctgcgtcggc caggcctgtt cgatgggcgc ggtgctgctg gcggcgggtt cgtccggcaa
     9901 gcgttatgcg ctgcccaatt cgcgggtcat gatccatcag ccactgggcg gcgcgcaggg
     9961 ccaggccacg gacatcgaca tccaggcgcg cgagatcctg tcgatgcgcc agcgcctcaa
    10021 cgagatcctg gcccaccaca ccggccagcc gatcgacacc atcgcccgcg acaccgagcg
    10081 cgacaacttc aagtccgccg aagcggcccg cgaatacggg ttggtggacg aggtgctggt
    10141 gcgccgcccg gacgagtcga tccaggccgg ctaaggccac gaaggggcgg cgcgcctgcc
    10201 ccgcaaagca cgtgctggag ccattccagg cgaattgagg ccccgcttgg cccggcgggg
    10261 gcgtatggta tcctcgggcc gaagccgcga agcgcggcga gtgagcaacg aatgagcgaa
    10321 gaccgtcagg gacgcaccac cggcgacagc accaagatcc tgtactgctc cttctgcggg
    10381 aagagccagc acgaggtacg caagctgatc gcgggtccca gcgtgttcat ctgcgacgag
    10441 tgcgtggagc tctgcaacga catcatccgc gaggaactgg aggagaaggc ccagtccacg
    10501 cgcagctcgt tacccaagcc acgcgagatc ctcgaggtcc tcgaccagta cgtgatcggc
    10561 cagcgccgcg cgaagaagac cctcgccgtc gcggtctaca accactacaa gcgcatcgag
    10621 agtcggcaga agtcgtcgga cgatgtcgaa ctggcgaagt ccaacatcct gctggtcgga
    10681 ccgaccggtt cgggcaagac gctgctcgcg gagacgctcg cgcgcatgct caacgtgccg
    10741 ttcaccatgg cggatgccac cacgctcacc gaagccggct acgtcggcga ggacgtggag
    10801 aacatcatcc agaagctgct gcagaagtgc gactacgacg tcgagaaggc gcagcacggc
    10861 atcgtctaca tcgacgagat cgacaagatc agccggaagt ccgagaaccc gtcgatcacc
    10921 cgcgacgttt ccggcgaagg cgtgcagcag gcgctgctga agctgatcga gggcaccgtg
    10981 gccagcgtgc cgccacaggg cgggcgcaag catccccagc aggaattcct gcaggtcgat
    11041 acccgcaaca tcctgttcat cgtcggcggt gcgttcgcgg ggctggacaa ggtgatccag
    11101 cagcgaagca gcgaagccgg cggcatcggt ttcggcgcca aggtgaagag cgttgagcgc
    11161 aaggccgaca tcggccaggt gctggcgcag gtcgagccgg aagacctgat caagtacggc
    11221 ctgatcccgg agttcgtcgg tcgcctcccc gtggtcgcga cgctggagga actcgacgag
    11281 ccggcgctga tgaagatcct caccgagccg aagaacgcga tcaccaagca gttcaggaag
    11341 ctgttcgaga tggaaggcgt ggagctggag atccgccccg atgcgctcgc cgccatcgcc
    11401 aagcgggcgc tgaagcgaaa gaccggtgcg cgcggtctgc gcacgatcgt cgaatccgtg
    11461 ctgctcgaca ccatgtacga cttgccgtcg ctggaaaacg tcagcaaggt ggtggtcgac
    11521 gaatcggtga tcgagcacaa gtccgagccc tacctgatct accagagcgc gccggcaccg
    11581 gcaaaggccg cgtctgcgga ctgaggatgc cgggacgatg tgaagaaggc cgccgaaagg
    11641 cggccttcgt gtttgcagtg atcgatgacc gcgcgcacca ggtgcgccgt ccgcttgcat
    11701 cccgctgcgg ataaccccat aaccgtggca tggcggcgcc ttcgcgccgc gcgtcgtgaa
    11761 tggagtccgc atgagcagtc ccgccgaaca tccctctgtt gccttgccgg tgctgccgct
    11821 gcgagacgtg gtggtgttcc cgcacatggt gatcccgctg ttcgtcggcc gcgacaagtc
    11881 gatccgcgcg ctcgacatcg cgatggaagg cgacaagcgc atcctcctgg tcgcgcagaa
    11941 atccgcggag accgacgatc ccggcgcgac cgacctgcat ccgatcggca cgctggcgca
    12001 ggtgctgcaa ttgttgaagc tccccgacgg cacgatcaag gtgctggtcg aaggcctggt
    12061 gcgcgccagg gtggatgggg tccgtgagga agacggcgcg ctgtcgggca atgccgacgt
    12121 ggtcgactcc gccgagaccc gcgagccgcg cgaggtcgag gcgatcgccc gttcgctgat
    12181 gtcgatgttc gagcagtaca tcaagaccaa tcgcaagctc ccgccggaat tgatgcagac
    12241 gctgtccggc atcgacgatc cctcgcggct ggcggacacc attgccgcgc acctgggcgt
    12301 gcgtctgagc gacaagcaga aactgctgga gttgctggac accgccgagc ggctcgaaat
    12361 gctggtgggc ctggtggatg gcgagatcga cgtccagcag atggagaagc gcatccgcgg
    12421 ccgggtcaag tcgcagatgg agaaatccca gcgcgagtac tacctcaacg agcagatgaa
    12481 ggcgatccag aaggaactcg gagacctgga cgacgcgccg aacgatctcg acgagatcgc
    12541 ccgcaagatc gccgccgccg gcatgccgaa gccggtggag gcgaaggcca aggccgagtt
    12601 cgccaagctc aagcagatgt cgccgatgtc ggccgaagcc ggcgtcgtgc gcaactacct
    12661 ggactggttg ctgggcgtgc catggaatgc gcgcagcaag gtgcgcaagg atctcaaggc
    12721 agcgcaggac accctggacg cggatcatta cggcctggaa aaggtcaagg aaaggatcct
    12781 cgaatacctc gcggtgcaga cgcgcgtggc caagatgaag ggcccgatcc tgtgcctggt
    12841 cggccccccc ggcgtcggca agacgtcgct ggggcagagc atcgccaagg ccaccaatcg
    12901 caagttcgtg cgcatggcgc tgggcggcgt gcgcgacgag gccgagatcc gtgggcaccg
    12961 tcgcacctat gtcggctcga tgcccggtcg gatcgtgcag aacctcaaca aggtcggcac
    13021 gaagaacccg ctcttcgtgc tggacgagat cgacaagatg tcgatggatt tccgcggtga
    13081 tccgtcgtct gccttgctcg aggtgctgga tcccgagcag aaccacacct tcaacgacca
    13141 ttacctcgag gtcgacctcg acctgtccga agtgatgttc gtggcgacct cgaactcgct
    13201 caacatcccg ggtccgttgc tcgaccgcat ggaagtcatc cggatccccg gttataccga
    13261 ggaggagaag atcaacatcg cgatgcgtta cctggtgccg aagcaactga aggcaaacgg
    13321 tttgcgcgag ggcgagctga agatcgcgga gaacgcgatc accgacatcg tgcgctacta
    13381 cacgcgcgaa tccggcgtgc gcaacctcga gcgcgagatc gccaagatcg cccgcaaggt
    13441 ggtcaaggag atcgccttga aggggccgca gccgaagggc agcaagccga agaccgtcag
    13501 tgtcaccaac aagaccctgg acaagtacct gggcgtgcag cgcttcgact tcggacgcgt
    13561 cgaggcggag aacgaagtcg gcctggtgac tggcctggcc tggacggaag ttggtggcga
    13621 cctgctgcag atcgaatcca cgctggtccc cggcaagggc gcgctgttgc tgacgggtca
    13681 gttgggcgac gtgatgaagg agtcggcgtc ggcggcgttg tcggtggtgc gagcgcgcac
    13741 cgagcggctg ggcatcgagc tcgacttcct gcagaagtac gacgtccacg tgcacgtgcc
    13801 ggatggcgcg accccgaagg acgggccgag tgcaggcatc gcgatggcga ccgcgctggt
    13861 gtcgatgttg accaagatcc cggtacgcca cgacgtggcg atgaccggcg agatcaccct
    13921 gcgcggtcgt gtcagcgcga tcggtggcct caaggagaaa ttgctggccg cgctgcgtgg
    13981 cgggatcaag acggtgatca tcccggacga gaaccgcaag gacctggtcg acctgccggc
    14041 agcggtgacc aggcacatga ggatcgtgcc ggtgaagtgg atcgacgaag tgctcgacct
    14101 ggcgctggag cggccgttgc cgggacgggg cgtgccgggc gaaggcactc aaccgccatt
    14161 gtcgaaaccc ggcgaacgca cgagcagcga tgtcaagcat tgactccgat ggtccacgcc
    14221 tttcgtcgga gtgagcctga aacccgcgcc agcgcttgct ttccgggttg catgcaaaaa
    14281 aaagcgctgg tataaagttc gcaaccgtgg cgtaccgcag ggtatgccgt ggcaagcccg
    14341 gaatcgaggc aggggagcgc cgctggatcc ggtcaccgaa tacttctgta ccccattccg
    14401 caagggagca atcaaacaaa tgaacaaatc cgaattcgtt gatgcgatcg ccgatggcgc
    14461 cgagctgacc aaggccgatg ccggccgtgc gctcgacgcg atggttaccg ccatcaccaa
    14521 ggcgctgaag aaaggcgaca cagtgaccct cgtgggcttc ggcaccttct cggtgcgcaa
    14581 gcgcggcgcg cgcaccggcc gcaatccgcg tactggcgag accatcaaga tcaaggcctc
    14641 gaagaatccg gcgttcaagg cgggcaaggc gttgaaggac gcggtcaact gatcgcagtc
    14701 attgcaccga aaggcccggc atcgccgggc cttttttgtt gggggcggtt ccgctggcgg
    14761 cgttgcgctg cgataatccg tcgaggggca cttagctcag cggtagagcg gctcctttac
    14821 acggagcggg tcgggggttc gatcccctca gtgcccacca aaaaagcatt gcgcgcacag
    14881 cagtcgcacg ctatgatgtt cgtctcagcg gagtggtagt tcagtcggtt agaatgctgg
    14941 cctgtcacgc cggaggtcgc gggttcgagt cccgtccact ccgccatcat gaggaagccc
    15001 tgcagccatg cagggctttt tttttttgcc cgccgccgcc ccgcaactag agcgaagcga
    15061 gccccgcctg ccggggcgcg ctagactagg cggctacgcc tcatcgcaac ctgaccatgc
    15121 tgcagacact ccgcgacaag acttccggct ggatcgccac ggtgatcctc ggcctgctga
    15181 tcatcccctt cgccttcttc ggcatggagt cctacctctc gcagaaggtg gatacctacg
    15241 ccgcgcgcat cacgcaggca ccttcctggt ggccgtccgc gccgcaggca tggccggtga
    15301 gctacctgtg gaagacccac gacatcgagg cgcaggacta ccgccagcgg ctggaaaccc
    15361 tgcgcatgcg ccagcgcgac gaacagggcg acaagttcga cggcaaggcg ttcgagtcgg
    15421 cggaaaacaa acgccaggtc ctcgacgaca tgatcgacga gcaactgatg cggcttgcgg
    15481 ccgaaagcga cggcatcgtg atcagcgatg cagagatccg caaggagatc gagcagatcc
    15541 ccgacttcca ggtcgatggc aagttcgacg cagatcgcta ccagttgctg ctggcatcgc
    15601 agaatccccc gcagtcgccg cgcacgtttg aacagaaggt gcgcgacggc ctgcaatacg
    15661 gactgatccc gagccgtctt gcgcgttccg ggttcgtcac tgatgtcgaa ctcgaccgac
    15721 tgatgcgcct gctgggcgaa cgtcgcgacg tgagtttcgt ggtgatgccg ccggcgccgg
    15781 tggacaacgc gccagtaacg cccgcgcaga tccaggcctg gtatgcggcg catgcgcgcg
    15841 aataccgtac cccggagacg gtgcgcctgg agtatgtcga ggtcgacgct agcacgctgc
    15901 ctgttccgct ggccgatgaa gctgcgttgc gcaagctgta cgacgagcag gctgccaagt
    15961 actccactgc agaacaacgt tcggtttcgc acatcctggt gcaggttgcg gcggatgcga
    16021 gcgatgccga caagaaggcc gcggaagcgc gtgcgaagaa gatcgccgag caggcgcggg
    16081 cgccgggcgc ggatttcgcc gcgctggcac gcgccaattc cgacgatgcc ggttccaagg
    16141 cagcgggcgg cagcctgggg tggctgacca agggcggcat gccggggccg ttcgacgaag
    16201 cggcattcgc gatgcgcgcg ggcgaggtgc gtggcccggt gaaaaccgat ttcggctggc
    16261 acgtgatcaa ggtcgatgaa ctgcgtggcg gcacccgcca gccgttcgag gccgtgcgcg
    16321 cgcaactgga gcaggaactg gtcgagggcg gccgtgagcg cgccttcaac gagctcagtg
    16381 gcaagctggt cgatgcgatc tacaagaacc cgaattcgct ggagccggcg gccaagtcgc
    16441 tcggcttggc ggtccagacc accccggcgt tctcgcgcaa tggcgggccc ggcatcgccg
    16501 gcgacgccaa ggtccttcgc gccgcgttct ccgagaccct gttgcgcgac ggaaccgcca
    16561 gcgatccgat cgagttgtca cccacgcgga ccgtgctgat ccgcgtgatc gaacacaagc
    16621 cggaagccgc gttgccgttg tcgcaggtcg gcaatgcggt cgtgatggcg atccgcgccg
    16681 accgccagcg caaggcggcg gaagcggccg ccgatgccct ggtcaaggcg gcgaaagcct
    16741 cgggcttgcc ggcagcggcg acggcggcca cgttgccgct cggcgaggcc aacgatgtcc
    16801 gtcgtggcag cccggtgcct tcgcgggagg ccgtggacgc gttcttccgc gtcccgcgcc
    16861 cgcaggacaa cctgatcccg gtcggcaaga ccgaggctga cggtcagttc atcgtgttcg
    16921 cgatccgcgc ggtgcgcgat ggcgacatcg gccaggtcac cgccgccgag cgcgaccagg
    16981 tgcgccagca gctgtcgcag gcgtcaggcc tccaggcgca gaaggccttc gtgcgcgcga
    17041 cgcgtgccaa gtaccagatc aaggttgcgg aagaccggct gtgaaatgaa gccgtgcgtg
    17101 ccggctgcgc ccggcatgct ggaacgaaaa cgcccggcat tgccgggcgt tttcgtttgc
    17161 gtcggcgcga cgccgtaccc gcgcgcgggt cattcgatcc cgctcatccc catgatgttg
    17221 tagccggcgt cgacgtaggt gatctcgccg gtgatgccgg ccgccaggtc ggagcacagg
    17281 aacgcggccg tgttgccgac gtcctcgatg gtgaccacgc ggcgcagcgg cgcgaccgcc
    17341 tcgaactggg tcagcatctt gcggaagttg gcgacgccgc tggcagccag cgtgcggatc
    17401 gggccggccg agatcgcatt gacgcgcgtg ccttccgggc ccagcgccat cgccatgtag
    17461 cgcacggtcg cttccagcga ggccttggcc acgcccatcg tgttgtagtt ggcgagcgcg
    17521 cgttcggcgc ccaggtaact cagggtcagc accgagccgt tgcggccctt catcagcggg
    17581 cgtgcggcct tgccgatcgc cgcaagcgaa tacgcgctga tgtcgtgggc gatgtggaaa
    17641 cgttcgcggt tgatcccatc caggaaatcg ccctcgatcg cctcgcgcgg ggcgaacgcg
    17701 atgcagtgga cgaggatgtc gaagccgtcg gcccagtgct tgccgagttc gtcgaaacag
    17761 gcgtcgatct ggccatcgtc ggccacgtcg agcggcagca cgatcttgct gccgtagttc
    17821 gccgcggcgt cctcgacccg cgatttcagc ttctcgttct ggtaggtcag cgccagttcg
    17881 gcgccttcgc ggtgcatggc atcggcgatg ccggtggcga tcgaacgctg gctggcgatg
    17941 ccggtgacca gtgcacgctt gccttgcagg aatcccatgg tgtctccttc ggtgccgcgc
    18001 ggggcgcgtc gagcaatcgc tctagtttgc ctcgtcgggc gcggtgcggc cagcatcggc
    18061 tccgatgggg gcgagcaccg cgcgcgcgcc gggatgccat cccgggttga gcgcgaggac
    18121 caggtccgcg tccaggccct gcgcgacggc ccattcacgc aggtcgcggc tgcctgccgg
    18181 cagacggcga ggagccagac gcggcacctg ccgttcgatc gcgcgtcgcc aacgcgcgtt
    18241 atcggcgcgc tggtcgaaca ggcaggcgat ggcgttcaac ttggcaggat agctgcgggt
    18301 gataccggac atgcgcgcgg cacctttccg gcgcgatgcg cgcaacgcgc cttcaccggc
    18361 gttgtaggcc atcaccgcct gccgccagtc gcgcccgaag aggcggtgga gtcgggcgag
    18421 gtagcgcacg gcagcctcgg tggacgccac caccgagaac cgttcatcca cggctccacg
    18481 cacggtcatg ccgtgccggc gcgcagtgcc ggcggtgaat tgccagagac ccgccggccc
    18541 accacggctc cgcgcatcgg cgcggtagcg gctttcgacg aacgggatca gcgcaaactc
    18601 gctcggcagc ccggcctcgc gcacctcgtc caaaacgtat ccgaacatgg cgagggcgat
    18661 ttcatcttcg tccgcgaggt gttccgcggc gcgcgcgtaa tggctacgcc agcgcgcgct
    18721 ggacttgcgg caatcggcgt cggccaagcc ggcgtggaag cgctgcagga tctcgccgcc
    18781 gctgcgggaa tgcggtgcgg aggatgcaag cccgagtccg ctggcgagca gcagggccag
    18841 cgccagggcg cgtgctgcct tcatccgcga aagccgtctt tccagcgacg cagggtggcg
    18901 aacacctccg gcggcgacgc cagtggatgg ccggcgtgcg tttccgcggc ggcatgcacg
    18961 gccgggtcgt tgcaacgcag gaacggattg cgggcgcgtt cgtcctcgag tgtggaaggc
    19021 aggctggatc gacctgctgc acggagcgcg cgaaccgtgt cggcgcgttt gcgcagcgcg
    19081 tcgttgccgg gatcgacggc gatggcgaat gcggcgttcg ccagcgtgta ttcgtgcccg
    19141 cagcagaccc gcgtcccggc cgggagtgcg gccaggcgtt gcaacgaggc gtgcatctgc
    19201 gccggggtgc cttcgaacat gcgcccgcag cccaggctga agagggtatc gccgcagaac
    19261 aacaggccgg tcccgtcgag gtcgtagcca tggaaggcga tgtggctgcg ggtgtggccg
    19321 gggatgtcga tgaccgtgaa gcgccatggg ccggcctcgg ccaggtcacc gtcaccaacg
    19381 cgtcgggtgg cgaacccgat gcgctcgtcc cgcggcgcta tcacgggcac gccgggccag
    19441 ttccgcagca attccgggac gccgccgatg tggtcgttgt gatgatgcgt cagcaggatc
    19501 gcgtgcggcg gcggtgcgcc ggccagcgca tcgagcactg gcgatgcctg gccgggatcg
    19561 acgaccagcg agccgccggc ggcgtcgcgc agcagccaga cgatgttgtc ctcgaacgct
    19621 ggaacctgtt gcagcggcat ttgtccagtc cgtgacgggt cgcgggcttt cccgcagaat
    19681 cctgcgggta tgtcgccgac cttgccgctg tccgtgttcc gccgtcaacc cgatgccacc
    19741 gccgcggagg cgtggttcgc cgggcgggcg gggcaggcga tcctggccag tcaggccgaa
    19801 tgcctgcgcg tcgcgctgca gcaacgcacg ggccacgctg cggcgtggct gcgcccggcc
    19861 gctgctgaag acgcctgcgt actggacgat gccgcgctgc tgcgcctgca accggaaggc
    19921 aacggattct caggcgacct gcggtgcgct ttgccgctgc cgttggccag tgaatcctgc
    19981 ggacttgccc tggtgcagca cgtcgtcgat gcctccgcgg atccgctggc cctgctggac
    20041 gaatgcgcgc gcgtgctgct gcccggcggt tggctgtggc tgctcgcgct gaacccgctg
    20101 tcgccgtatc gctggcgctg gcgtgggcag ggcttgcggg tctccgagcc ggtgacctgg
    20161 cggcgccgct tgcgcgcggt cgggctggtg cccgatgcgg tctcgcaggg attggggccg
    20221 acctgggagg tcgcccacga cccctgcctg caggatggcg cgggcctgcg cgccgcgttc
    20281 ctgctgcgcg cgcaaaagcg ccggctgccg atgacgcccg gccgcgccca gcgcgtgctg
    20341 cggctgcagg caggttcggc atgacggcgc gcaagcggat cgaagtgcat accgatggcg
    20401 cctgcctggg caatcccggg ccgggtggct gggcggcgtt gctgcgctgg cgcgaggtcg
    20461 agcgcgagct ctccggtggc gaggccgata ccaccaacaa ccgcatggaa ctgatggcgg
    20521 cgatcgccgc gctggaggcc ctgcgcgagc cctgcgatgt catcctgacc acggattctc
    20581 agtacgtgcg ccagggcatc ctggactggc tggccaactg gatccggcgc ggctggaaga
    20641 cttccggcgg cgatccggtc aagaaccgcg acctgtggga acgcctgcag gccgcatccg
    20701 cgccgcacaa gatcgactgg cgctgggtga aggggcactc gggacacccc gagaacgaac
    20761 gcgtcgacgt gctggcgcgc accgcggccg aacagatcag ggacaaggca ggctgatgcg
    20821 ccaggtcgta ctggataccg aaaccactgg cctggaatgg cagaagggca accgcgtggt
    20881 cgagatcggc tgcgtggagc tggtcgagcg ccgccccacc gggcgcacct tccatgctta
    20941 cctcaacccg gatcgggaca tggagccggg ggcccaggaa gtcaccgggc tgacccgcga
    21001 gttcctggcc gacaagccgc gctttgccga ggtggccgac gaattcctcg ccttcatcga
    21061 cggcgccgag ctggtgatcc acaacgcggc gttcgacatc ggcttcctcg atgccgaact
    21121 ggcccggctg gaaggcgtgg gcaagatccg cgaccgctgc gcggtcgaag acaccctgct
    21181 gatggcccgc cagcgctttc cgggccagcg caattcgctg gacgcgctgt gcaagcggct
    21241 gggggtggac aactcgcagc ggcaatggca tggcgcgctg ctcgacgcgc agatcctgct
    21301 ggacgtctac atcgcgctga cgtcgggcca gagcgagatc ggcttcgcca gcgaggccgc
    21361 cccgcgggcc gccgaaacgg tgcaggtggt ggcccgctca tccgaccctt cgttgcggcc
    21421 acgggtgcag gtcacgaccg aggatgccgc cgcgcatgcc gcgcgcctgg cgggactgca
    21481 gaagaaagcg ggccgctgcc tgtggctgga gatcgaacag gccggatgaa accgccctgg
    21541 gtcagtggct gcggaccagc accacggtga cgttgtccga gccgccgcca tcgagcgcgg
    21601 ccgcgaccag cccatccacg cattcctgtg cactgcattc ctggtgcgcc agcacgcgtg
    21661 cgatgctgcg gtcgtcgact tcctcggtca gcccgtcgct gcagagcagg agctgcatgc
    21721 cgggacggag ctcgccgccc agggtttcca cgttgagttg cacggggtcg gtgacgccca
    21781 gtgcctgggt caccacgttg cgatgcgggt ggctgcgcgc ctggtcggcg gtgatcgcgc
    21841 cctgggcgat cagttcctgt acgtagctgt gatcctgcga gatctgggtc agcgcgccgt
    21901 cccgccacag gtagacccgg ctgtcgccga cccaggctac ctcgaagcgg ttgccgttga
    21961 cccgtgcggc caccacggtg gtgcccatcg gcaggctgtc gccgcggcgc ttggagcaac
    22021 ggatgatttc ctcgtccgcg gtgcggatcg cctccgcgag cgtgttgccg gcacgtacct
    22081 cgcggacgat cgcctcgcgc gccagtgcgc tggccacttc gccgtactcg tgcccaccca
    22141 tgccgtccgc gaccagccac agccccagct cgctgtcgcc gtaataggtg tcctcgttca
    22201 actcccggcg caggccgaca tgggtcaggt gtccgaattc gatcatgcgg gcggcattcg
    22261 tccggggttt ggagggggta acgggatcgt acggggaaac cggacggggt tggtgatgct
    22321 gcgtgttgcg aggtggaggg cgtgcgaggg tcggatgtgt tggttgaacg gcgggataga
    22381 cgcgggccat ccatggccct cgcgctgcgc gcgacctgcg gtcgtcctga tcggcagtcc
    22441 tgccgatcgg tcgaaccccg cggaggtttc tcaccaaccc tctccgccag acacaaaaaa
    22501 cgccccgtgg gggcgttgtt tgtgtctggc ggagaggggg ggattcgaac ccccggtagg
    22561 ctcacaccta cgcctgattt cgaatcaggt acattcaacc gctctgccac ctctccggga
    22621 agctggggtg tcgcgggccg gcaatcatac gagccgggac gccggcaggc aaggcgctac
    22681 actggcgccc cgcctgcaac tggtgccgcg atgtccgaaa tcctcgtccc cgtctcgttc
    22741 ggcgaactgc tggacaagat cgccatcctg cagatcaagt cggagcggat gagcgatccg
    22801 gccaagctgg ccaacgtgcg caacgagctg tcggcactcg acacgacctg ggacgcgcat
    22861 ccggcctcga agcaggacat cgatgccctg cgcgccgacc tcaaggcggt caacgagcgc
    22921 ctgtgggtga tcgaggacga catccgcctg caggaaaagg cgcaggcctt cgacgccgaa
    22981 ttcatccgcc tggcgcgtgc ggtgtacttc gagaacgaca tccgcgcgcg gatcaagaag
    23041 gacatcaacc tggcgctggg atccagttac gtggaagaga agtcgtacca ggattacggc
    23101 accggccagg gcgcctgatc acccgatccc ggcgcgatag cgctcgaacg ccgccaccgc
    23161 gtcctcgacg gtgaccaggt ccatcacgcc ctcggcctcg atcctggttc cccacttcaa
    23221 ttcgctggcg ggcttgccgc gatacgtgcg cgcggcagcg tcgtaacggt ccacgcagaa
    23281 gcggcggtcg gaatacggtc cgctgcgatg cggattggtg gccgcatgca ggcccagcac
    23341 cttcgtcccc atcgcgttgg cgatatgcat gggcccggaa tccggggtga ccagcaggtc
    23401 ggcccgggcg agcagggcgg gcaattgctt cagggtgtcc ttgccgatca ggtccagcac
    23461 cccatcggct gaggtcatcg ccgcgaggat cgcgttgccg gtcgtgcgtt cgagatcgct
    23521 gcggccgccg cacagcacga cgcgccaacc ggccgacacg gcgtggtcgg ccaaggccgc
    23581 atggcgctcg gcgcgccagt tgcgcaggac gtggctggag cagggcgaga ccatcagtac
    23641 ggggcggcca tcgtcttccc actgcgcccg cgcccatgca tgcgcgtcgt ccggcaccgg
    23701 caaattccag aggacgccgt cgcgcaccag ccccagcggt tcgcagaagc tgccgatcgc
    23761 atccagcacg tggatgccgg ggcggttggg gatgcgcgcg ttgatgaaca ggccatggcc
    23821 ttccttgctg cgcgaccggt cgtaaccaac gcgcagccgc gccgggatga acgcggacag
    23881 caggttcgcg cgcgccgata cctgcagctg caacaggacg tcgaagcgtt gcccgagttc
    23941 gcggcgcagc gcacgcatgc cggccagccc ggtcttcttg tcgtactcga cgaagcgcac
    24001 gcccggcagg ccagccagca gccggtgttc gcccttgccg atgacccacg tgatcgcgac
    24061 atcgggccat gcactgcgca atgtgtgcaa caacggcaac acatgggtga catcgccgag
    24121 cgcggacagg cgcagcaggc agagtgaacg gggcgcggcg gggtttggca cgtgcggtta
    24181 aactcgcatg gatgactggt ttcgacgcca acgaacacct gacgccgttc cgcgacgcgc
    24241 gcggctacgg ggcgattctg ttcgaccgca cccaattgcg ccaacccgac cccgcctggt
    24301 tctcgccgca gcactggggc gaggccgcgc agccggtcgc cgaaggtggc cgcggcggcg
    24361 cctggttcat ccagaccggc gagggcgacg cggtgctgcg ccagtacctg cgcggcggtt
    24421 gggccgccag cctgagccgc gacgggcatt tgtggcgcgg gatcaaccgc gtgcgcagct
    24481 ttgccgagtt ccgcctcctg cgcgcgttgc gcgagaagaa gcttcccgtg ccgatgccgt
    24541 tcgcggcgta ctaccgccgc gagggcctgc actatcgcgc atcgatcctc atgcagcgcc
    24601 tgccgggcgt gcgttcgctg gccgcgctcg cggccggaga cgagccgccg tgggagcagg
    24661 ccgggcaatt ggtcgcgcgc ttccaccgcg ccggcctgga ccatgccgac ctcaatgcgc
    24721 acaacatcct gttcgatgcc gacggccgcg gctggatgat cgatttcgac cgctcgcgcc
    24781 tgcgcatccc cgcgacggcg tggcggcagg ccaacctgca gcgcctgctg cgttcgctgc
    24841 acaagcgtca tggttcgcgc agcctggtcg aggtggatgc cggtttcgcc cgcctgcgcg
    24901 ccgcttatga cgccgcctgg cagcggggct actgagctgg acaccgcgat gtcgccgccg
    24961 cgcgatgcgt tggcgcggcc actgcgcgac ctgcggctgt cggtgatcga cgcctgcaat
    25021 ttccgctgcc cgtactgcat gccggccgac cgcatcgccg acgaccatgg cctggacgcg
    25081 gcctcgcggt tgtcgttcca cgagatcgaa accctggtgc gcggcttcgc ccggcttggc
    25141 gtgcgcaaat tgcgcctgac cggtggcgag ccgctgttgc gcaagcgcct gccggagctg
    25201 gtggcgcggc tggccgtgat cgatggcatc gaagacctgg cgctgaccac caacgggtcg
    25261 ctgcttgccg cgcacgcgcg tgccttgcgc gaagccggcc tgacccggat cacggtcagc
    25321 ctggacacgc tggatcccga tgcgttccgc gcgctgtccg gtgggcgcgg cgagctgcgc
    25381 gacgtgctgg cggggatcga ggcggcgacc gccgccggct tcggcccgat caagctgaat
    25441 tgcgtcgtcc agcgcggggt gaacgagggt ggcatcgaaa cgctggcgtc attcgcgcgc
    25501 gaacaccacc acgtgctgcg tttcatcgaa tacatggacg tgggcacctg caacggctgg
    25561 cggcgcgaac gcgtggtgcc gtcggcggaa ctccgcgatc gcatcgccgc gcattggccg
    25621 ttgcgcgcgc tggatgcgaa ctaccgcggc gaggtggccg agcgctatgc ctacgacgac
    25681 gggcgcggcg aggttggctt cgtcagctcg gtcacggcgc cgttctgcgg cgactgccat
    25741 cgcgcgcgtg tctccgcgga cggcatgctc tacacctgcc tgttcgccgg gcagggccac
    25801 gccctgcgcg gcgtgcttgc gcggggcgag gacgcgttgg ccgcacgggt cgccgacctg
    25861 tggtcgaagc gtgccgatcg ctacagcgaa gtgcggagcg aggccagcac ctcgcgcaag
    25921 catgtcgaga tgtacctggt cggcggctga ggcggcgata atccccgcat gacgaagaag
    25981 cgagcgacgc tcacccacct cgaccccacg ggtcgcccgg cgatggtcga tgtctccgcg
    26041 aagccggtga ccgcgcgcca ggcgcaggcc ggatgccggg tgacgttccc gcccgcgatc
    26101 gccgcacagc tgcgcgacaa cggcctgaag agcgccaagg gcggcatcgt cgacaccgcg
    26161 atcatcgccg gcacgatggc ggtgaagaag acccacgaac tgatcccgtt ctgccatccg
    26221 ctgccgatcg atggctgccg cttctcgatc gactggcacg atgcacgcac gctgcggatc
    26281 gaatgcacgg tcggcaccac gcatcggacc ggggtggaga tggaggcgct gaccggcgcc
    26341 accgtcgccg cactgacggt gtacgacatg tgcaaggcgc tgagccacgc catcgtgatc
    26401 ggcccggcga agctgctcgg caagcgtggc ggcaagcgcg acatcggcca gttggcatga
    26461 gcacacgggt cgaagtgttg tatttcgccg ccttgcgcga tgccgccggc atcgccagcg
    26521 agcatgtcga caccggtgcg gcggacctgg ccgggttgta tgccgaggcg cgggtacggc
    26581 atccattgcc gttcgcgcag acccaactgc gggtcgccgt ggatggcgcg ttcgcggggt
    26641 ggggcgatgc ggtgcgggct ggttcgacag tggcgttcat cccgccggtg tcggggggct
    26701 gagatggccc atttccggct atcggatgca ccgatcgacg ccgttgcgct gcagtccggc
    26761 ctgcgcgacc cgcgtgccgg cgcctgcgcc agcttcgagg gctgggtgcg cgaccacaac
    26821 gacgggcgtg ccgttgacgg cttgcgctac gaggcctacg tcagcctggc cgaagccgag
    26881 ggcgcggcga tcctggccga ggcgctggaa cgcttcgaca tcgtgcaagc cacctgcgtg
    26941 caccgcgtcg gcgagctggc catcggcgat ctcgcggtct gggtgggtgc cagcgccgcg
    27001 catcgcggtc cggccttcga ggcctgccgc tggatcatcg acgaggtcaa ggcacgggtg
    27061 ccgatctgga agcacgagcg ctatgcaggc ggcgacgccg gctggctgca ccccggcccg
    27121 tgaccggctt ccttgtcccg gggcggggca gccgctagac tgcgcgaccc ggaatgtcgc
    27181 cgcgacgcgg catggcgggt tccagcggag aggtgtccga gcggttgaag gagcacgcct
    27241 ggaaagtgtg taagcgtcta aaccgcgctt cgggggttcg aatccccctc tctccgccaa
    27301 ttagaattct aaacccctga tttcaggggt tttttatttt ctaccgctgt gcggtatgcc
    27361 atccagtatg ccacaagtgt ttggttccga ttgggtggct ttgtggtcct aaattccgac
    27421 ctgcaaatgc tcgcgagcgt gggctgacat gg
//
LOCUS       DAOOCX010000017        27281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1424_length_27281_cov_8.065783, whole genome shotgun sequence.
ACCESSION   DAOOCX010000017 DAOOCX010000000
VERSION     DAOOCX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 27281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27281
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1424_length_27281_cov_8.065783"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PK399_02960"
     CDS             <1..628
                     /locus_tag="PK399_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW12111.1"
                     /translation="PTTTKKTSTNVDSPSQRAGTDPPARGLQGGERALIAAVLALIAL
                     LAGLDLMVDQREGVTAWHLLVEAAVVGVAGLGAFHLLRGAWRLRKRLDMQDRDFLAFR
                     QQAEAWRNDSRKYVDGLSRSIDLQLDRWTLSAAEKEVAFLLLKGFSLRDIAQARGTSE
                     KTARVQSSAIYAKSGLAGRSELSAFFLEDLLPPRVTPPDDAPGDPQPR"
     gene            775..1044
                     /locus_tag="PK399_02965"
     CDS             775..1044
                     /locus_tag="PK399_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12112.1"
                     /translation="MSQHHIPLFTFTLGSTESISPDALRAMWVNASGSGNVSVGRRNP
                     EGTWRDRPIYTLYATQGLANLPEVENRLRRALEVAHLHATVTSLH"
     regulatory      1094..1271
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            1276..1854
                     /locus_tag="PK399_02970"
     CDS             1276..1854
                     /locus_tag="PK399_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HPW12113.1"
                     /translation="MSAFLTATGAVAIAEIGDKTQLLSFILAARFRKPLPIILGILVA
                     TLANHALAGAAGAWVMSMTGPGVLRWILGVLFIAMAAWTLVPDKFDESEVPKASRFGV
                     FGTTVVAFFIAEMGDKTQIATAAMAATEASLVAIVAGSTLGMMLANVPAVFIGDRLLH
                     KVPMGLVRAIAAGLFALFGVLILLGAGSKLGF"
     gene            1949..2494
                     /locus_tag="PK399_02975"
     CDS             1949..2494
                     /locus_tag="PK399_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12114.1"
                     /translation="MAAQQMQHRNTRKGNPWRLAVWGAAAGLLMVPAIAMQVGADGVH
                     WTTLDFVVMGTLLGACCLAYEVATRLSGNTLYRLGFAVAILLGFLLVWINLAVGIIGG
                     EANPANLVFACVLAVGLVGALIGRFTAGGMSRAMVATGIAQLLAGGYALALGSMEGGM
                     LSAAFAAMWFTSAALFHKAAR"
     gene            2542..>3554
                     /locus_tag="PK399_02980"
     CDS             2542..>3554
                     /locus_tag="PK399_02980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPW12115.1"
                     /translation="MTTKAYGAHAADKPVEAIAIERRAPGANDVQIDIAYCGVCHSDL
                     HTVRSEWPGTLYPCVPGHEIVGHVSAVGGAVSGFKVGDVVGVGCLVDSCGECGACHDH
                     LEQFCEKGMIGTYNGPTPDAPGHTLGGYSQRIVVDQGFVLHVRHPVEQLAAVAPLLCA
                     GITTYSPLRHWNAGPGKKVGVVGIGGLGHMGLKIAHAMGAHVVAFTTSESKREEALKL
                     GADEVVNSREAGQMQAHAGSFDFILDTVAAPHNLDAFTGLLKRDGTLCLVGAPASPHP
                     SPSVMGLIFGRKSIAGSLIGGIAETQEMLDFCAEHGIVADIEMIAARDIEPSYERMLK
                     SDV"
     assembly_gap    3555..3654
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3655..4304)
                     /locus_tag="PK399_02985"
     CDS             complement(<3655..4304)
                     /locus_tag="PK399_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW12116.1"
                     /translation="MPSLLIADDHPLFRAALRQAARDALGEVELLEAGDLDGVLATLD
                     AQPQVDLVLLDLHMPGNHGLAGLAAVRAQHPGVAVIVVSANDDPRVVRRALDHGAAGY
                     LPKSAGLDELRDAIRTVLACETWLPAMLRASVSRATSSPHDADLAARLASLSPQQFRV
                     LTLVAEGLLNKQIADRLDVQERTVKAHLTAIFERLGVRNRTQAGVVLRELELSDPAR"
     gene            complement(4313..5122)
                     /locus_tag="PK399_02990"
     CDS             complement(4313..5122)
                     /locus_tag="PK399_02990"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HPW12117.1"
                     /translation="MTDRFLEGRTALVTGSTSGIGLAIAQALAAAGARVAINGLGSPE
                     QIAAAITDVDAAGGGETKHFDADLRDPVAIEAMMSDLADWSGGGIDILVNNAGIQHAV
                     PLHEMPVAKWNDIIAINLSSAFHTMRAAMPMMASRGFGRVINVASVHGLVASKDKAPY
                     VASKFGVVGLSKVAALEYAAQGSRDSGGITVNCICPGWVETPLIEPQIEARIGGGTRD
                     DGVRALLSEKQPSLRMTLPAEIGALAVFLCRREAHNITGTAMPVDGGWTAQ"
     gene            5247..7025
                     /locus_tag="PK399_02995"
     CDS             5247..7025
                     /locus_tag="PK399_02995"
                     /inference="COORDINATES: protein motif:HMM:NF022073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HPW12118.1"
                     /translation="MNRLQSCACIAAILLLGACASTTPSGSRMFSQPRSSDHRGQDDL
                     LTAGLGLPGLRAMTPPGFADAARPTPQELRRRALWANWRGIADLAPGGGYGELYGSVA
                     SVPGREFSAFATVPGARQPHRVLLQLPDDFDIKKRCVVVAASSGSRGIYGAIAVAGAW
                     GLPRGCAVAYTDKGAGTDYFDLDAGQGIDAAGQVAGADAELAFKPAAGGSGVAYKHAH
                     SQDNPEADWGRHVRQAAEFALATLNEQLPQQARFTFANTRVIAVGISNGGGAVLRAAE
                     LEGDWLDAVVAGEPNVLAQGHGARDLYDYSTEAALLMPCALPALGLPAAPGADARCAA
                     LAAQGMLSGADLKAQQQDALAKLHASGWTDPALRAGSISVGFDLWRAVGVGYASAYGR
                     YAPDAHPCGYRYSALGVDLSPRAATDVERASWWADASGIPPGAGVGIVDPNPAADLGL
                     KGLQCLRRLWTGDSADAQRVRGGIAETRAGLPRAGLPVIVLHGTDDGLVPQAFSSAPY
                     VAMAQAAGRDVRYWQVRNAQHFDAFLGFPQYGAVYLPLLPYVYEALDRVDAHLDGKGA
                     LPVDAVIATVPRAGKPLATDNLAMPR"
     gene            7089..7721
                     /locus_tag="PK399_03000"
     CDS             7089..7721
                     /locus_tag="PK399_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPW12119.1"
                     /translation="MKQRHFSMLRDFHLADWFTLANAFCGTGAVFAAMRFLQDGAASE
                     GNVRDLLIGMALIPLAFVFDALDGRIARWRKVSSTLGRELDSLADVISFGVAPAALAY
                     ACGLQGGWDWIVLSYFVGCGVSRLARYNVTAETLADGGDKVEYFEGTPIPTSVAIVGL
                     LAWLAWSDRIGAALPFGALQLGPWQLHPLVLVFALSGSLMISKTIRIPKP"
     gene            7787..8875
                     /gene="phaE"
                     /locus_tag="PK399_03005"
     CDS             7787..8875
                     /gene="phaE"
                     /locus_tag="PK399_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaE"
                     /protein_id="HPW12120.1"
                     /translation="MAHDSNDFEALARRYWDMWGDAARAATPGVGGFGAMGSMGGLQG
                     FRDAMDGWTRNVGAGSSGANDVLGHFNRQNQAWYGQMQQLAAQFAGQPHSARDVAEAW
                     RKLVGGNGNELFQGLFDGMQGPGMQQLQQWSDAAAPWLQSMQKEAATTLGMPTFGFAR
                     EHQERLQGLARAQLDWQQALGAYQALLGKVSQDAFSRFESKLIDREEPGRQVGSVRAL
                     FDLWVDAAEEAYAETALSVEYRHAYGTLVNAQMGLRKAMQSLIEQNASMAGLPGRTEL
                     DSAHRKIAELERLVRRMQRKGDADDEAASPPPVRKVAAKPAPAPKPAAQKATARKPIA
                     KKISRPVAKKTARTLKTSGKPAANRRTR"
     gene            8877..9947
                     /locus_tag="PK399_03010"
     CDS             8877..9947
                     /locus_tag="PK399_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HPW12121.1"
                     /translation="MTGPLNITAESLALEADQFQRKLGAGLGTLREMDDVQFGVTPRE
                     EVWRDGKVVLYRFKGERTPTAKVPMLVCYALVNRPYMVDLQADRSLVKGLLERGEDVY
                     IIDWGYPDRSDRYLTLEDYIERFLGGAVDHLRKAHGLPSVNLLGICQGGVFSLCYSAL
                     HPRKVRNLITMVTPVDFHTADNMLSNWNREIDVDLLVDTLGNIPADMMNLTYLMLKPW
                     RLFAQKYVGMVDILDDRKAMEDFLRMEKWIFDSPDQAGEAFREFNTQFFQENRFVKGG
                     AQVGGREVHLGLVEMPILNIFAEQDHLVPPDASRALRDLAGTRDYTELSFKGGHIGIY
                     VSGRAQKEVPQTIHEWLAKRSR"
     gene            9962..10414
                     /locus_tag="PK399_03015"
     CDS             9962..10414
                     /locus_tag="PK399_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12122.1"
                     /translation="MPRWLLRCLQVLGATWTSPNTVLGLLAGAASLARGAHWHWRGQD
                     LALVFHRVPWGKGGALTLGNVILHTGDTLDSLCLTYAHQAGLGKEAPIRIGDHERAHV
                     YQYMVLGPLFLPLYLAFGGVSVRNRFERAADRYAQSGRGWWPFRRTAS"
     gene            complement(10645..11091)
                     /locus_tag="PK399_03020"
     CDS             complement(10645..11091)
                     /locus_tag="PK399_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006430887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPW12123.1"
                     /translation="MTKTSMTLLAVACALALSACGGSEKAAEGEHAATETAHASSSAG
                     MPNGHIEAGKALASKKGATGQACVDCHGADGNSPIDATYPKLGGQYADYIEHALQAYR
                     SGDRGGSTTTDLMGSQAKELTDQQIADLAAYYGSVTGQLRDLHNAE"
     gene            complement(11100..11510)
                     /locus_tag="PK399_03025"
     CDS             complement(11100..11510)
                     /locus_tag="PK399_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPW12124.1"
                     /translation="MRPLSIAASLALVLVASAVTAQNAATPPAASDPAAVAAPAAEMA
                     PAAAPAAALAGDATRGKGLTYTCRGCHGVTGYKNAYPSYHVPKLGGQSDVYLRNALME
                     YRGAKRKHPTMQAQAESFSEQDIADIAAYLSSIK"
     gene            11720..12874
                     /locus_tag="PK399_03030"
     CDS             11720..12874
                     /locus_tag="PK399_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW12125.1"
                     /translation="MHDTTQAAAPRTGHFLPIARLGLLAMMLALAACKGGKPGDGKGE
                     DGKDAANKGPEAVPVEVAKAGRRSIAASYAGSAPLEARGEAQVVAKTSGIALRVFADV
                     GQQVRAGQPLVQIDRDRAALQVAQADAQVRKLEANYRRASQLVEQKMVSVNDVEQLRY
                     DLENARASLRLARLELSYGTVNAPISGVVATRNIKPGNLVQINTPIFTIVDNSRLEAT
                     LNAPEREIETLKAGQTVQLSVDALPGKTFAGRIDRVSPVVDSGSGTFRVICAFDGGGL
                     LQPGMFGRIRIEYDQRANALVIPRTALLEDGSSPAVYTVKADKATRVALKLGYVDGEW
                     VEVRDGLRQGDPVVIAGKAALREGSAVQVIGAGASTKSVATTAPAQAAAQ"
     gene            12871..16368
                     /locus_tag="PK399_03035"
     CDS             12871..16368
                     /locus_tag="PK399_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW12126.1"
                     /translation="MSPAEPKKDGAYDPLASPGGFSLVEFATRRRVTIFMMTLTLVLF
                     GLISLGSLKVNLLPDLSYPTLTVRTDYTGAAPSEIETLISQPAEEALGVVKGLRKLKS
                     ISRTGQSDVVLEFAWGTNMDQASLDVRDKMEALQFPLEAKPPVLLRFNPSTQPIMRLA
                     LSPKKDGGSDADNIRQLMELRRFADDDLKRKLEPVEGVAAVKVGGGLEDEVQVDIDQR
                     KIAQLGLSLDTVINRLQQENVNVSGGRLEEGSQRYLVRTVNQFATVDEIANMLLTTRT
                     ASAAGGGSEQAMRLAAASGDASVMAALSQQGGSASSGSLPVRLRDVADVRQGYKEREA
                     IIRLNGREAVELAIYKEGDANTVSTADALDAKLKTIREQLPDDIDVVTIEDQSVFIRN
                     AIGDVKLDAVIGGLLSILIIFLFLRDGWSTFVISLSLPVSIIATFFFMGQLGLSLNVM
                     SLGGLALATGLVVDDSIVVLESIAKARERGLGILEAAIVGTREVSMAVVASTLTTIAV
                     FLPLVFVQGIAGQLFRDQALTVAIAIGVSLVVAMTLIPMLSALKGRPPLGFPEEARAP
                     GWQPTKAWQKPAAYTGKGIGAMFRYAFFSIAWLITRIWRGLAAVIGPVMRKASDLAMA
                     PYARAERGYLRILPNALARPTPVLLVAGIAFALTLAAVPMLGTDLIPQFAQDRFDMTA
                     KLPPGTPLAQTDALIRDVQGKHAKDEGVRVLYGVSGTGTRLDASPTESGENIGKLSVV
                     MADNEAEASLTEALRKTMQAHPSAQVDFSRPELFSLSPPLEIEVEGSDLEGIRVAGAK
                     LAAMLRANPHYADVKSTVEQGFPEIQIVFDQDRAAALGLTTRQIADVVVKKVRGEVAT
                     RYSFRDRKIDVLVRAQESDRASVDDIRRLIVNPGSGAPVELAAVAEVVSTTGPSEIHR
                     ADQRRVAIISANLRDIDLGNAIREVEAMVAKTPLGTDIDMRIGGQGQELGESIRSLLF
                     AFALAIFLVYLVMASQFESLLHPFVILFTIPLALVGAVAALLLTRAPVSVVVFIGLIL
                     LVGLVVKNAIILIDKVNQLREDGVAKREALVEGARSRLRPIMMTTLCAVFGFLPLALA
                     FGEGAEVRSPMATTVIGGLLVSTLLTLLVIPVVYDLLDRKPDEFYAERGRRARNAAAQ
                     VAEVLSHEHDALGEGADKA"
     gene            16373..19447
                     /locus_tag="PK399_03040"
     CDS             16373..19447
                     /locus_tag="PK399_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW12127.1"
                     /translation="MNITELSIRRPVTTIMLFVSMVVIGLIASFRLPLEAEPEATIPF
                     FFVSLPYSGSTPEEVERNLVRPVEEALATMPGIESMNSRANAEGGSVQVRFSDWTRDI
                     AIAASEARERIDAIRDDLPDDFRRYFVYRWSTSDREAISLRLTSQVDLTTRADLIERS
                     IKQRLERLPGVARVEVEGVAKQEVLVALDKDRLTAHGVALNELVQQLQTANFSVSAGQ
                     ITEAGQRLRVQPKGELLELQQLRDLRIDRRGTRLSDVADVELKPQRMDYVRRMDGKPS
                     VGVDIYKERSANLVDLSRGVRAEIAELEKDPAMGGINIEVTDDQGLAVTSSLLALAEA
                     GGIGLLLSVIVLYAFLRHWPSTLMVSTAIPICFVMTLGFMYFTGISLNIISMMGLLLA
                     VGMLVDNAVVVVESIYQEREKYPDRPWLASIIGTRHVAIALSAGTLCHCVVFLPMMFG
                     EKNIITIYLGQLAATISFSLLASWLVAISLIPMLSARMKTPPALKSPFITAMQARYAR
                     ALRWTLGHRGWSVAGILAISLLSIYPMTHTQGGGDDNDPSEINIFYQWKGAYSKEEMG
                     KEVARVEAFVNANRKPFHVERVYSRYSEQGWAQTRLFLDIEDPEQSKKIAEDVRKGLP
                     KSARANLGIGWPGGQGNEGEGITFSLIGDSTQTLQELAGDILPMLARNPKLRDVRIDT
                     GDANTELRVQVNRERAAAYGFSAREVAQFVGVALRGSSLRDFHREDVEIPVNVRFAGS
                     DDYGMEDLSTFMVRAPNGSDVPLLAMVDVGTSPAATSIQRQSRQTMLQVQAGLAKDVS
                     VEDARKAIETTLNGTQFPPGYSYTFDGAGFNINFDGMDQMMRAIMIALVLMLVIMAAV
                     FESLLFPLAIMSCVLFSIFGVYWLFWLTGTEMNIMAVIGILVLMGVVVNNGIVMIEHI
                     NNLRRRGLPRTEALIEGSRERLRPIMMTMGTAILAMIPIALSTKTAEGMPPYYPMARA
                     IAGGLAFSTVVSLLFLPTIYAMLDDLRMGSGNLVRRARMNRAQRRDAKAAAA"
     gene            19444..20136
                     /locus_tag="PK399_03045"
     CDS             19444..20136
                     /locus_tag="PK399_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HPW12128.1"
                     /translation="MSTGGELRGDGRLAGALYLIVVLAGMHCLAYVPGQVGETLAQAS
                     AHAGMFRSGIASFLVMQVAFLLLPLALYRVLGDVDRRAAIAMVALAVVSVPIGLVAVT
                     HRMEALALLDQAAAGTTDAMEAAFALSLQRYGHGLRVASLFWGLWLLPFGWLVLRSGR
                     LPRVLGLLLVLGGLGYVAQVFGGLVPGLAEASWLRYVRMPAAFGEIGSCLWLLVFGAR
                     AGRVDPVRTAAS"
     gene            20133..20558
                     /locus_tag="PK399_03050"
     CDS             20133..20558
                     /locus_tag="PK399_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HPW12129.1"
                     /translation="MTIPVSPSRELADPPLPTRLKLSALWASLTFCYLYGDYFGLYKP
                     GKLQHMLEGAGPMGPTSQGSLLFVALLLVVPGLMVFLSLALPARPVRWLNIGLAVFYA
                     AFVAITMPGAWWFYLAYSSIEILLGAVIVWLAWRWPRTA"
     gene            complement(20565..21779)
                     /locus_tag="PK399_03055"
     CDS             complement(20565..21779)
                     /locus_tag="PK399_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPW12130.1"
                     /translation="MTTESNSAADAFAGQPVVELERDGVHYTLLGTAHVSRTSIDAVM
                     AAIDTGRFDAVAVELDEQRHKALTHPDALAQLDLVKVIRERKIAPFAANLALAAYQRR
                     LAEQLGIEPGAELKAAAVEAAARGLSLQLIDRDVGITFRRILQGLSWWDRMKLVGGLA
                     GGLFASDEVSEDDIERLKEGDMLESSFGEFARDTPSLYATLIDERDQYMAAKLRERSD
                     GATHVLAVVGAGHLKGMARYLTEDQREPAELTAQLAHVVKKRNIPWITIILMLLICGG
                     IAWGYFNGGRELGRELLLQWVVWTGGLAGLGALLARGHVLSILAAAISAPLKPFRPGL
                     PPGMFSALVEVHLRKPAYPDFLALRDDAQTLGGWYRNRVCRVVLVFLLTNIGSMIGVW
                     VSGAAIIGKLMG"
     gene            complement(21864..22739)
                     /locus_tag="PK399_03060"
     CDS             complement(21864..22739)
                     /locus_tag="PK399_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPW12131.1"
                     /translation="MPLVRLIFALGGPIAPEAMVAKAARLFTTPFASSRSRARAAPTL
                     GAREDDLGIDGITLHTYAWGDPATQPYILFAHGWSSHGTRVARWIQPLRDAGYAVVTF
                     DQAAHGRSGGIHTTLPDFTCHLMAVAKHYGPAAAVIGHSLGGAATALALARGMQAKRA
                     VLIAPAADPLAAVERFSNLLWLASHLGRRMFARFEGAMRFSADELQAQHNAPRIGRPA
                     LIVHDIEDREVPWSEGERWARYWPQSRLLSTRGLGHNRIADDEGVIASALRFLRGESI
                     GERVVSTADLPYGLA"
     gene            complement(22811..23416)
                     /locus_tag="PK399_03065"
     CDS             complement(22811..23416)
                     /locus_tag="PK399_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW12132.1"
                     /translation="MNPLTATSKGAATRDAIIEKAYEIARFAGIEGLSIGPLAQAVGM
                     SKSGVFAHFGSREDLQLAVLESAGQRFGEFVLGAALSQPRGLPRLRAMMRNWFEWGRL
                     ETGGCVLVASVSEFDDRPGPLRDQVVLNERRWRQALQRAAQLAIDCGHLRDGDTDQYA
                     FELYAIPLAMLHEAGLFGYDQARRHGDSALERWFAAHAPSP"
     gene            complement(23483..24370)
                     /locus_tag="PK399_03070"
     CDS             complement(23483..24370)
                     /locus_tag="PK399_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HPW12133.1"
                     /translation="MSKYNTLPVALIQERNHGDAAANLAVIEARVSEAAKSGAKLVLL
                     QELHNGAYFCQHESVDEFDLAERIPGPSTERLAALAKQHGVVLVSSLFEKRAAGLYHN
                     TAVVFDADGSTAGKYRKMHIPDDPGFYEKFYFTPGDLGFTPIDTSVGRLGVLVCWDQW
                     YPEAARLMALAGAELLLYPTAIGWDPADDSAEKTRQRDAWVLSHRGHAVANGLPVLSC
                     NRVGHEPSPLGASGIDFWGNSHVLGPQGEFIAEAGTGPTILLAEVDMARSEHVRRIWP
                     FLRDRRIDAYGDLTKRYRD"
     gene            complement(24370..25413)
                     /locus_tag="PK399_03075"
     CDS             complement(24370..25413)
                     /locus_tag="PK399_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPW12134.1"
                     /translation="MQDIVRFPAEWEPQAAILIAWPNAETDWAERLAEVEETYIALVA
                     AIVRFQRVVICVADDDIETYAEMRLRSNRVDMDRVRFATVPYDDTWLRDSGPITLRDG
                     DAFRLLDFRFTGWGGKYGASDDDQLVQRLHANGLFGDAARDEVDFALEGGAIDTDGDG
                     TLLTTWQCLHERHPDASREELTTRLAGWLRQDRVLWLDHGALEGDDTDAHIDTLARFA
                     GRDAIVFQACDDPADSHYADLKAMGEEIAALRTREDRPYRLFPLPWAKPVIDIATDGS
                     RRRLAASYANFLIVNGAVLMPAYGDPADNAAAAVLADAFPDREIVQVPCRPLIWQNGS
                     LHCITMQLPEGLV"
     gene            complement(25422..25778)
                     /locus_tag="PK399_03080"
     CDS             complement(25422..25778)
                     /locus_tag="PK399_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12135.1"
                     /translation="MNRLSRSILSVFAATLVCAPAIALAQQAQVQGDVLLIERVQEEP
                     GNLPSRGMSMSQVEARFGAPSDRLDPRGGQKRQWPTINRWTYPAFTVYFERSKVIDVV
                     MTKADPNEIGPKPPTT"
     gene            complement(25959..26084)
                     /gene="ykgO"
                     /locus_tag="PK399_03085"
     CDS             complement(25959..26084)
                     /gene="ykgO"
                     /locus_tag="PK399_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HPW12136.1"
                     /translation="MKVLSSLKSAKNRHRDCKVVRRRGKVFVICKSNPRFKARQR"
     gene            26276..26959
                     /gene="cmk"
                     /locus_tag="PK399_03090"
     CDS             26276..26959
                     /gene="cmk"
                     /locus_tag="PK399_03090"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPW12137.1"
                     /translation="MDPNAPVLTIDGPSGSGKGTISRIVAQRLGWHYLDSGALYRAVG
                     VAAGWADLDLDDAGALVRCTFDTLVGFREGDAGDLRVLVNGHDATDELRTETAGAAAS
                     AIAAIPEVRAALKDRQRAFRQLPGLVADGRDMGTVIFPDAGHKVFLTASADERAERRY
                     KQLKDKGVSVTLDGLLREILARDARDASRAVAPLRPADDAVRIDTTGLGIDGVVALVL
                     DVVSRPRAH"
BASE COUNT         4265 a         8945 c         9442 g         4529 t
ORIGIN      
        1 gccgacgaca acgaagaaga cgagcacgaa cgtcgatagt ccgtcgcagc gcgcgggcac
       61 cgatccgccg gcgcgcggcc tgcagggcgg cgaacgtgcg ctgatcgccg cggtacttgc
      121 cttgatcgca ttgctcgcgg gcctggacct gatggtcgac cagcgcgagg gcgtgacggc
      181 gtggcacctg ctggtcgagg ctgcggtggt cggggtcgcc ggcctgggcg ccttccacct
      241 gctgcgtggt gcatggcgcc tgcgcaagcg cctggacatg caggatcgcg atttccttgc
      301 gttccggcag caggcggagg catggcgcaa cgattcgcgg aaatacgtcg acggattgtc
      361 ccgctccatc gacctgcaac tggatcgttg gacgttgtct gccgccgaga aggaagtcgc
      421 gttcctgctg ctcaaggggt tcagcctgcg cgacatcgcg caggcgcgcg gcacctcgga
      481 gaagaccgca cgggtgcaat ccagcgcgat ctacgcgaag tccggcctgg cgggcaggtc
      541 cgagctgtcc gccttcttcc tggaggacct gttgccgccc cgtgtaaccc cgccggacga
      601 tgcgcccggc gacccgcagc cgcgctagat cggcggatcc ggatccgtcg cccgcgatcc
      661 cttgtcccat gcgcagcgtc tccggatcgc cgcgcaggac gtccgaacgt ggtggctgcc
      721 ttcgcgggtg ggttccgcaa tggcgcatac tcgtgcgcca agtggagcac ctgcatgtcg
      781 cagcaccata tcccgttgtt caccttcacc ttgggcagca cggagagcat cagcccggat
      841 gcattgcgcg cgatgtgggt caacgccagc ggcagcggca atgtcagcgt cgggcgcagg
      901 aatccggaag ggacgtggcg cgacaggccg atctataccc tgtatgcgac gcagggcctt
      961 gccaacttgc cggaagtcga aaatcgcctg cgtcgcgcgc tggaagtggc gcacctgcac
     1021 gcaacggtga ccagtctgca ttgacctgac gcgccgccgg gtttttgctt gaggccgcgc
     1081 aacagggtgg cttacactgc gcgcgtcctt ggggagtagc ctctcgccgc cccggcgagg
     1141 cccgcgtcaa catgcttggc ctcaatcggc cgtggcgcgt gcgatcccgt atctggcaag
     1201 accatcgacc acgaccccgc ctgaaggccg ggggagcgtc gtggtcatgc gtcctttccc
     1261 ggccggttac ttccaatgtc cgcattcctc accgccaccg gcgctgtcgc catcgccgag
     1321 atcggcgaca agacgcaact tctctcgttc atccttgccg cgcgtttccg caagccgctg
     1381 ccgatcatcc tcggcatcct ggtcgccacg ttggccaatc acgcgctcgc cggtgccgcc
     1441 ggtgcctggg tcatgtcgat gaccggtccc ggcgtgctgc gctggatact cggcgtgctg
     1501 ttcatcgcga tggccgcctg gaccttggtg ccggacaagt tcgacgaatc cgaggtgccg
     1561 aaggccagcc gtttcggtgt gttcggtacc acggtggtcg cgttcttcat cgccgagatg
     1621 ggcgacaaga cccagatcgc caccgcggcg atggcggcga cggaagcttc gctggtggcc
     1681 atcgtcgccg gcagcacgct gggcatgatg ctggccaacg tgccggcggt cttcatcggt
     1741 gatcgcctcc tgcacaaggt gccgatgggc ctggtgcgcg ccatcgcggc tgggttgttc
     1801 gcgttgttcg gcgtgctgat cctgctgggc gcgggcagca agctgggctt ctgacgccgg
     1861 cggacttcgt ccccgcttca cacgatgctc gccgggcctt cgcggcgggc gtgcaaggtg
     1921 gcaccactta tccgcgaggg gcaaggcgat ggcagcacaa cagatgcaac accgcaatac
     1981 gcgcaagggc aatccgtggc ggctggcggt gtggggcgcg gcggcagggt tgctcatggt
     2041 cccggccatc gcgatgcagg tcggtgccga cggcgtgcac tggacgacat tggatttcgt
     2101 ggtgatgggg acgttgctgg gcgcgtgttg cctcgcctac gaagtggcca cgaggctgtc
     2161 cggcaacacg ttgtaccggc tgggcttcgc agtcgcgatc ctgctcgggt tcctgctggt
     2221 gtggatcaac cttgcggtcg gcatcatcgg tggcgaagcc aatccggcca acctggtatt
     2281 cgcctgcgtg ctcgctgttg gactggtcgg cgcattgatc ggtcgtttca cggcgggcgg
     2341 catgtctcgg gcgatggtgg cgaccggtat cgcccagttg ctggcaggcg gctacgcact
     2401 ggcgctcggt tcgatggaag gcggcatgct cagcgcggca ttcgccgcga tgtggttcac
     2461 gtcggccgcc ttgttccaca aggcggcgcg gtaacaactg caggcgcata cttcgggcat
     2521 cccagtcccg gagtcctgcg catgaccacg aaagcctacg gcgcccacgc cgccgacaag
     2581 cccgttgaag cgatcgccat cgagcgccgc gctcccggcg cgaacgacgt gcagatcgac
     2641 atcgcctatt gcggcgtctg ccactccgac ctgcacaccg tgcggtcgga atggcctggc
     2701 accctgtatc cctgcgtccc gggccacgaa atcgtcggcc acgtcagcgc ggtcggcggc
     2761 gcggtcagcg gcttcaaggt cggcgatgtc gtcggcgttg gctgcctggt cgacagctgc
     2821 ggcgagtgcg gcgcctgcca cgaccatctg gagcagttct gcgagaaggg catgatcggc
     2881 acctacaacg ggccgacacc ggatgcgccc ggccataccc tgggtggata ctcgcagcgc
     2941 atcgtggtcg accagggctt cgtcctgcac gtccgtcatc cggtggaaca gcttgccgcg
     3001 gtcgcgccgc tgctgtgcgc cggcatcacc acctattcgc cgctgcgcca ctggaacgcc
     3061 gggccgggca agaaggtcgg cgtggtcggc atcggcgggc tgggccacat gggcctgaag
     3121 atcgcgcatg ccatgggcgc gcacgtggtg gccttcacca ccagtgaatc caagcgcgag
     3181 gaagcgctga agctcggtgc cgatgaggtc gtgaactcgc gcgaggccgg gcagatgcag
     3241 gcccatgcgg gcagcttcga tttcatcctc gacaccgtgg ccgcaccaca caacctggac
     3301 gcgttcaccg gcttgctcaa gcgcgacggc accctctgcc tggtcggcgc accggccagc
     3361 ccgcatccaa gcccgagcgt gatgggcctg atcttcggcc gcaagtcgat cgccggttcg
     3421 ctgatcggtg gcatcgccga aacccaggag atgctggatt tctgcgcgga gcacggcatc
     3481 gtggcggaca tcgagatgat cgccgcacgg gacatcgagc ccagctacga gcgcatgctc
     3541 aagagcgacg tgaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgcgcc
     3661 ggatccgaca gttccagttc gcgcagcacc acgcccgcct gggtgcggtt gcgcacgccc
     3721 agccgttcga agatcgcggt caggtgggcc ttgaccgtgc gctcctgcac gtccaggcgg
     3781 tcggcgatct gcttgttgag cagcccttcc gcgaccaggg tcagcacgcg gaactgttgc
     3841 ggcgagaggc tggccaggcg cgcggccagg tcggcatcgt gcggcgagga cgtggcgcgc
     3901 gacacgctgg cccgcagcat cgccggcagc caggtttcgc aggccagcac cgtgcggatc
     3961 gcatcgcgca gttcatcgag gccggcgctc ttcggcaggt agccggcggc accgtggtcg
     4021 agtgcgcggc gcaccacccg cggatcgtcg ttggcggaca ccacgatcac cgcgacgccc
     4081 ggatgctgcg cacgcacggc agccaggcct gccagtccgt ggttgccggg catgtgcagg
     4141 tccagcagga ccaggtcgac ttgtggctgc gcatcgagcg tggccagcac gccgtcgagg
     4201 tcgccggctt ccagcaattc gacctcgccc agcgcatcgc gcgccgcttg ccgcagggcg
     4261 gcgcggaaca gcggatggtc atcggcgatg agcagcgaag gcatggggtc gcttactgcg
     4321 cggtccagcc accatcgacg ggcatcgcgg taccggtgat gttgtgcgcc tcgcgccggc
     4381 acaggaacac cgccagcgca ccgatctcgg cgggcagggt catgcgcagg ctgggctgct
     4441 tctcggacag cagggcacgc acgccatcgt cgcgggtgcc gccaccgatg cgtgcctcga
     4501 tctgcggctc gatcagcggc gtttccaccc agcccgggca gatgcagttg acggtgatgc
     4561 cgccggagtc gcgcgacccc tgcgccgcgt attcgagcgc ggcgaccttg ctcaggccga
     4621 cgacgccgaa cttgctggcc acgtacgggg ccttgtcctt cgatgccacc aagccgtgta
     4681 ccgatgccac gttgatcaca cgaccaaagc cacgggaagc catcatcggc atcgccgcac
     4741 gcatggtgtg gaacgctgaa gacaggttga tcgcgatgat gtcgttccat ttcgcgaccg
     4801 gcatctcgtg cagcggcacg gcgtgctgga tgccggcatt gttgaccagg atgtcgatgc
     4861 cgccgccgga ccaatcggcc aaatcgctca tcatcgcctc gatcgccacg gggtcgcgca
     4921 ggtcggcgtc gaagtgtttc gtttcgccgc cgcccgcggc atcgacatcg gtgatcgccg
     4981 ccgcgatctg ttccggcgag cccaggccgt tgatcgcaac ccttgccccg gcggcggcca
     5041 gggcctgcgc gatggccagg ccgatgccgg aggtgctgcc ggtcaccagg gcggtgcggc
     5101 cttcgaggaa tcgatccgtc atgtgtctcg tccgtgctgc attgcgtcag ctgcatacgc
     5161 tagcaggccg ggccgcagga aggggcgggc tgggcgtggt acgaaggtac gatgcgggcg
     5221 cgccgcgtag tcgctaaggt cgcggcatga acagactcca gtcctgcgcc tgcatcgccg
     5281 ccatcctcct gctcggcgcc tgcgccagca ccacgccatc gggaagccgc atgttcagcc
     5341 aaccacgcag cagcgaccac cgcgggcagg acgacctgct taccgcggga ctgggattgc
     5401 ccggtctgcg cgcgatgacg ccgccgggct tcgccgatgc cgcgcgtccg accccgcagg
     5461 aattgcgccg ccgcgcattg tgggcgaact ggcgcgggat cgccgacctc gcgcccggtg
     5521 gcggttatgg cgagctgtac ggcagcgtgg ccagcgtgcc cggtcgcgag ttcagcgcct
     5581 tcgcgacggt accgggagcg cgccagccgc atcgcgtgtt gctgcagctg ccggatgatt
     5641 tcgacatcaa gaaacgctgc gtggtggtcg cggcctcgtc cggctcgcgc gggatctatg
     5701 gcgccatcgc agtcgcaggg gcgtggggcc tcccgcgcgg ctgcgccgtg gcctataccg
     5761 acaagggcgc gggcacggac tatttcgacc tggacgccgg acagggcatc gatgccgccg
     5821 gccaggtcgc gggtgccgat gcagaactgg cgttcaaacc ggcggccggt ggcagcggcg
     5881 tcgcctacaa gcatgcgcat tcgcaggaca accccgaggc cgactggggc cgccacgtga
     5941 ggcaggcggc ggagttcgcg ctggctacgc tcaacgaaca actgccgcag caggcgcgct
     6001 tcaccttcgc caacacccgc gtcatcgcgg tcggcatctc caatggcggc ggcgcggtcc
     6061 tgcgtgcagc cgaactcgaa ggcgactggt tggatgccgt cgtcgccggc gagcccaacg
     6121 tgctggccca gggccacggc gcgcgcgatc tgtacgacta cagcaccgaa gccgccttgc
     6181 tgatgccttg cgccctgcct gcgctgggtt tgcccgcggc gcctggcgcg gatgccaggt
     6241 gcgcggcact ggctgcgcag ggcatgctct ccggtgccga cttgaaggca caacagcagg
     6301 acgcactggc caagctgcat gcaagcggct ggactgatcc agcgcttcgc gcgggctcga
     6361 tcagcgtcgg tttcgacctg tggcgtgcgg tgggcgtggg ctatgcatct gcctatggcc
     6421 gctacgcccc tgacgcgcat ccctgcggct accgctattc ggcactgggc gtggacctgt
     6481 cgccgcgcgc cgccaccgat gtcgaacgag catcatggtg ggccgatgcc agtggtatcc
     6541 cgcccggcgc gggcgtcggc atcgtcgatc ccaatccggc ggctgatctt ggtttgaagg
     6601 gattgcaatg cctgcgccgc ctgtggaccg gcgacagcgc cgatgcgcaa cgcgtgcgcg
     6661 gaggaatcgc cgaaacccgc gccggcctgc cacgcgccgg cctgccggtg atcgtgctgc
     6721 acggcaccga cgacggcctg gtgccgcagg cattttccag cgcgccgtac gtggcgatgg
     6781 cgcaggccgc cggccgcgac gtgcgctact ggcaggtgcg caatgcccag catttcgacg
     6841 ccttcctcgg cttcccgcag tacggtgccg tgtacctgcc gttgctgccg tatgtgtatg
     6901 aggcgctgga tcgcgtggac gcgcacctcg atggcaaggg cgcattgccg gtcgacgcgg
     6961 ttatcgccac cgtgccgcgt gccggcaagc cgttggccac cgataacctg gcgatgccac
     7021 gctgatcgtg cttgccggct tcacgccggc tttggcagtc ttcggatctg cgcgcgcggg
     7081 agcgaacgat gaagcaacgc catttctcga tgctgcggga tttccatctc gccgactggt
     7141 tcacccttgc caatgccttc tgcggcaccg gcgcggtgtt cgccgcgatg cgcttcctgc
     7201 aggacggtgc ggcttcggaa ggaaatgtcc gcgacctgct gatcggcatg gcgctcatcc
     7261 cgctggcctt cgtgttcgat gccctggacg gccgcatcgc ccgctggcgc aaggtgtctt
     7321 cgacgctcgg tcgcgagctg gattcgctcg ccgacgtgat cagcttcggt gtcgcccccg
     7381 cggccctggc ctatgcctgt ggcctgcagg gcggctggga ctggatcgtg ctgagttact
     7441 tcgtgggctg cggggtgagc cggctggcgc gttacaacgt gaccgcggag acgctcgccg
     7501 acggcggcga caaggtggaa tatttcgagg gcacgccgat cccgaccagc gtggccatcg
     7561 tcggcctgct ggcgtggctg gcctggagcg atcgcatcgg cgcagccttg ccgttcggcg
     7621 cgctgcaact cgggccgtgg caattgcatc cgctggtgct ggtgttcgcg ctgtcgggtt
     7681 cgctgatgat cagcaagacg atccgcatcc ccaagccctg aaccgcgcga tggcggtgtc
     7741 accggcgcgt tgctagcatg cgggctcccg ccgcggatga gccgagatgg cacacgacag
     7801 caacgacttc gaggcgcttg cgcgccgtta ctgggatatg tggggcgatg cagcgcgtgc
     7861 ggcgacgccg ggcgtgggcg gcttcggcgc gatgggttcg atgggtggcc tgcagggatt
     7921 ccgcgacgcg atggacggct ggacccgcaa cgtcggtgcg ggcagcagcg gcgccaacga
     7981 tgtgctcggc catttcaatc gccagaacca ggcctggtac ggccagatgc agcaactcgc
     8041 ggcgcagttc gccgggcaac cgcacagtgc gcgcgacgtc gccgaggcct ggcgcaagct
     8101 ggtcggaggc aatggcaacg agctgttcca gggcctgttc gacgggatgc agggccctgg
     8161 catgcagcag ttgcagcagt ggagcgatgc cgcggcgccg tggctgcagt cgatgcagaa
     8221 ggaggccgcg accacgctgg gcatgcccac cttcggcttc gcccgtgaac accaggaacg
     8281 cctgcaggga ctggcgcgcg cccaattgga ctggcagcag gccttgggtg cgtaccaggc
     8341 gttgctgggc aaggtctcgc aggatgcgtt ctcgcgcttc gaatcgaaac tgatcgatcg
     8401 cgaggagccg gggcggcagg tcggcagcgt gcgtgccctg ttcgacctgt gggtggatgc
     8461 ggcggaagag gcgtatgccg agacggccct gtccgtcgag tatcgccacg cctacggcac
     8521 cctcgtcaac gcgcagatgg gactgcgcaa ggccatgcaa tcgctcatcg aacagaacgc
     8581 cagcatggcc ggcctgccgg gtcgcacaga actggacagt gcgcatcgca agatcgcgga
     8641 actggaacgt ttggtgcgac gcatgcagcg caagggggac gcggacgatg aagctgcctc
     8701 gccacctccg gtgcgcaagg ttgcagcgaa gcccgcaccc gcgccgaaac ccgctgcgca
     8761 gaaagccact gcaaggaaac ccatcgcaaa gaagatttcc aggccggtcg cgaagaaaac
     8821 cgcgcgcacc ttgaagactt ccggcaagcc cgccgccaat cgcaggacgc gctgacatga
     8881 ccggacccct gaacatcacc gcggaatcgc ttgcgctgga agccgaccag ttccagcgca
     8941 agctcggcgc gggcctgggc acgttgcgcg agatggacga cgtgcagttc ggcgtcaccc
     9001 cgcgcgagga agtgtggcgc gacggaaagg tcgtgctcta ccgcttcaag ggcgaacgca
     9061 cgcctaccgc gaaggtgccg atgctggtct gctacgcgct ggtcaaccgc ccgtacatgg
     9121 tcgacctgca ggcggatcgc tcgctggtga agggcctgct ggagcgcggc gaggacgtgt
     9181 acatcatcga ttggggctac cccgatcgca gcgacaggta cctcacgctg gaggactaca
     9241 tcgaacgctt cctcggtggt gccgtcgatc acctgcgcaa ggcgcacggc ctgccgtcgg
     9301 tgaacctgct cggcatctgc cagggcggcg tgttttcgct ctgttattcg gcgctgcacc
     9361 cacgcaaggt caggaacctg atcaccatgg tcacgccggt ggacttccac accgcggaca
     9421 acatgctgtc gaactggaac cgcgaaatcg acgtcgacct gctggtcgac acgctgggca
     9481 acatcccggc ggacatgatg aacctcacct acctgatgct gaagccgtgg cggctgttcg
     9541 cgcagaagta cgtgggcatg gtcgacatcc tcgacgacag gaaggcgatg gaagatttcc
     9601 tgcgcatgga gaagtggatc ttcgattcgc ccgaccaggc cggtgaggcc ttccgcgaat
     9661 tcaataccca gttcttccag gaaaaccgct tcgtcaaggg cggcgcgcag gtcggcggcc
     9721 gcgaggtgca cctggggctt gtcgagatgc cgatcctcaa catcttcgcc gaacaggacc
     9781 acctggtgcc gccggatgcc tcgcgcgcgc tcagggacct ggccgggacg cgcgactaca
     9841 ccgagttgtc gttcaagggc ggccatatcg gcatctacgt ctccggtcgt gcgcagaagg
     9901 aagtgccgca gaccatccac gagtggctgg ccaagcgctc gcgttgaacg acatgggcga
     9961 catgccgcgg tggctgctgc gttgcctgca ggtgctgggc gcgacgtgga catcgccgaa
    10021 cacggtgctc gggctgttgg caggtgctgc atcgctcgcc cgcggcgcgc attggcattg
    10081 gcgcggccag gatctcgccc tggtgttcca tcgcgtgccc tggggcaagg gcggggcgct
    10141 gacgctgggc aacgtgatcc tgcacaccgg cgataccctc gattcgctgt gcctgaccta
    10201 tgcgcaccag gccgggctgg gcaaggaagc gccaatccgc atcggcgacc acgaacgcgc
    10261 gcatgtgtac caatacatgg tgctgggccc gctgttcctg ccgctgtacc tggcgttcgg
    10321 cggggtgagc gtgcgcaacc gcttcgagcg cgccgccgat cgctacgccc agagcggtcg
    10381 cggttggtgg ccgttccgac gaacggcatc ctgaacgggg aacgcttagc cgattcctaa
    10441 cattcgcgag cgtagcctgt gcccgtcgaa gaaaaacttc gggttgcgca aggctccccg
    10501 gaaaatcgct tcggcatacc ggacccggtc ccctccaggc gcaaagcccg cgagaccggg
    10561 tctttctttt tcgcccggcg gcatctcgcg cgcaaacgaa aggggccttg cggccccttc
    10621 ggttcgacac gatgcgctga acgcttattc ggcgttgtgc aggtcgcgca attggccggt
    10681 gacgctgccg tagtaggccg ccaggtcggc gatctgctgg tcggtcaatt ccttcgcctg
    10741 gctgcccatc aggtcggtgg tggtgctgcc gccgcggtcg cccgagcggt aggcctgcag
    10801 ggcgtgttcg atgtagtcgg cgtactggcc gcccagcttc gggtaggtcg catcgatcgg
    10861 gctgttgccg tcggcgccgt ggcagtccac gcaggcctgg ccggtcgcgc ccttcttcga
    10921 ggccagcgcc ttgccggcct cgatgtggcc gttcggcatc ccggcggagg acgacgcatg
    10981 ggcggtttcg gtggcggcgt gttcgccttc ggccgccttc tccgacccgc cacaggcgga
    11041 cagggcaagt gcgcaggcaa cggcgagcag ggtcatggag gtcttggtca tggcgcggct
    11101 cacttgatgc tggacaggta ggcggcgatg tcggcgatgt cctgctcgga gaagctctcc
    11161 gcctgcgcct gcatggtcgg gtgcttgcgc ttggcgccgc ggtattccat cagtgcgttg
    11221 cgcaggtaga cgtcggactg gccacccagc ttgggcacgt ggtagctcgg gtacgcattc
    11281 ttgtaaccgg tcacgccatg gcagccgcga caggtgtagg tcaggccctt gccgcgggtg
    11341 gcgtcgccgg ccagcgcggc cgcgggtgcg gcggccgggg ccatttcggc tgccggggca
    11401 gcaacggcag cgggatcgct ggcggcgggc ggggtggcgg cattctgcgc ggtaacggca
    11461 gaggcgacga gcaccaaggc aaggctggcg gcgatcgaca acggtcgcat catgtggcgg
    11521 ttccggattc gaggagcggg ttggatgccg tgcaagcggc atgcatcggg caagtatagc
    11581 gagggagccg gccgcttcat attcgcggca tgcgcatcga cgatcatgcg cgcgatcgcc
    11641 agaccatgac cttcggcgca tgccaaggtg ctggtgatag gtgagatact tgactacaac
    11701 accagacagg ctcctctcca tgcacgacac cacccaggcc gccgcccccc gcaccggcca
    11761 tttcctcccc atcgcgcgcc tgggcctgct ggccatgatg ctggcgctgg cggcctgcaa
    11821 gggcggcaag ccgggcgacg gcaagggcga ggatggcaag gacgcggcca acaagggccc
    11881 cgaggccgtg ccggtggagg tcgccaaggc cggacgccgc tcgatcgccg ccagctacgc
    11941 cggcagcgcg ccgctggaag cgcgtggcga ggcgcaggtg gtggccaaga cctcgggtat
    12001 cgccttgcgc gtgttcgccg acgtcggcca gcaggtgcgc gccggccagc cgctggtgca
    12061 gatcgaccgt gaccgtgccg cgctgcaggt ggcgcaggcg gatgcgcagg tgcgcaagct
    12121 ggaggccaac taccggcgcg cctcgcagct cgtcgagcag aaaatggtca gcgtcaacga
    12181 cgtggagcaa ctgcgctacg acctcgagaa tgcgcgcgcc agcctgcgcc tggcgcgact
    12241 ggaactctcc tacggcacgg tcaacgcgcc gatctccggc gtggtcgcta cccgcaacat
    12301 caagccgggc aacctggtgc agatcaacac gccgatcttc accatcgtcg acaactcgcg
    12361 gctggaagcg acgctcaacg cgcccgagcg cgagatcgaa accctgaagg ccgggcagac
    12421 ggtgcagctc agcgtggatg cgctgccggg caagacgttc gcgggccgca tcgaccgggt
    12481 gtccccggtg gtggattccg gcagcggcac gttccgcgtc atctgcgcgt tcgacggtgg
    12541 cggcctgctg cagcccggca tgttcgggcg catccgcatc gagtacgacc agcgcgccaa
    12601 tgcgctggtc atcccgcgca ccgcgctgct ggaagacggc agttcgcccg cggtgtacac
    12661 ggtcaaggcc gacaaggcca cgcgggtcgc gctgaagctg ggctatgtcg atggcgaatg
    12721 ggtcgaggtg cgcgatggcc tgcgccaggg cgatccggtc gtgatcgccg gcaaggcggc
    12781 gctgcgcgag ggcagcgcgg tgcaggtgat cggtgcaggc gcgtccacca agtccgtcgc
    12841 cacgacggca ccggcgcagg cggccgcgca atgagtccgg cggaaccgaa gaaggacggt
    12901 gcctacgacc cgctggcgtc gcctggcggg ttcagcctgg tcgagttcgc cacccgccgg
    12961 cgcgtcacca tcttcatgat gacgctgacc ctggtgctgt tcggcctgat ctcgctgggc
    13021 agcctcaagg tcaacctgtt gcccgacctc agctatccca cgctgaccgt gcgcaccgac
    13081 tacaccggcg cggcgccatc ggagatcgaa accctgatct cgcagcccgc cgaggaagcg
    13141 ctgggcgtgg tcaagggcct gcgcaagctg aagtcgatca gccgcaccgg gcagagcgac
    13201 gtggtgctgg agttcgcgtg gggcaccaac atggaccagg ccagcctgga cgtgcgcgac
    13261 aagatggagg cgctgcaatt cccgctggag gccaagccgc cggtgctgct gcgcttcaat
    13321 ccgtcgaccc agccgatcat gcgcctggcg ctgtcgccga agaaggatgg cggcagcgat
    13381 gccgacaaca tccgccagct gatggaactg cgccgcttcg ccgacgacga cctcaagcgc
    13441 aagcttgagc cggtcgaagg cgtggccgcg gtcaaagtcg gtggtggcct ggaggatgaa
    13501 gtgcaggtcg acatcgacca gcgcaagatc gcccagctcg gcctgtcgct tgacaccgtg
    13561 atcaaccgcc tgcagcagga gaacgtcaac gtctccggtg gtcgcctcga ggaaggctcg
    13621 cagcgctacc tggtgcgcac ggtcaaccag ttcgcgacgg tcgatgaaat cgccaacatg
    13681 ctgctcacca cgcgcaccgc gtcggcggcc ggcggcggct ccgagcaggc gatgcgcctg
    13741 gccgcggcca gcggcgacgc cagcgtgatg gcggcgctct cgcagcaggg cggcagcgcg
    13801 tcgtccggca gcctgccggt acgcctgcgc gatgtcgccg acgtgcgcca gggctacaag
    13861 gagcgcgagg cgatcatccg cctcaatggc cgcgaggcgg tcgaactggc gatctacaag
    13921 gaaggcgacg ccaacacggt gtctacggcc gatgcgctgg acgccaagct gaagacgatc
    13981 cgcgagcaac tgcccgacga catcgacgtg gtcaccatcg aagaccagtc ggtcttcatc
    14041 cgcaatgcca tcggcgacgt caagctggac gcggtgatcg gcggcctgct gtcgatcctg
    14101 atcatcttcc tgttcctgcg cgatggctgg agcacgttcg tgatctcgct gtcgctgccg
    14161 gtgtcgatca tcgcgacgtt cttcttcatg gggcagttgg gcctgagcct caacgtgatg
    14221 tcgctgggcg gactcgcgct cgcgacgggc ctcgtcgtcg acgactccat cgtggtcctc
    14281 gagtcgatcg ccaaggcgcg cgaacgcggg ctgggcatcc tcgaggcggc gatcgtcggc
    14341 acccgcgaag tgagcatggc ggtggtcgcg tccacgctca ccacgatcgc ggtgttcctg
    14401 ccgctggtgt tcgtgcaggg catcgccggg cagttgttcc gcgaccaggc actgaccgtg
    14461 gcgatcgcca tcggcgtgtc gctggtggtg gcgatgaccc tgatcccgat gctgagtgcg
    14521 ctgaagggtc gcccgccatt gggtttcccg gaggaggcgc gcgcgccggg ctggcagccg
    14581 accaaggcct ggcagaagcc ggccgcgtac accggcaagg gcatcggcgc gatgttccgc
    14641 tatgccttct tcagcatcgc ctggctgatc acgcggatct ggcgcggcct tgcggcggtg
    14701 atcggtccgg tgatgcgcaa ggccagcgac ctggcgatgg cgccttacgc gcgcgccgaa
    14761 cgtggctacc tgcgcatcct gccgaatgcg ctggcacggc cgacgccggt cttgctggtc
    14821 gccggcatcg ccttcgcgct gaccctggcg gcggtgccga tgctgggcac cgacctgatt
    14881 ccgcaattcg cccaggaccg gttcgacatg accgccaagc tgccgcccgg cacgccgctg
    14941 gcgcagaccg atgcgctgat ccgcgacgtg cagggcaagc acgccaagga cgagggcgtg
    15001 cgcgtcctct acggcgtgtc cggcaccggt acccggctcg atgccagccc gaccgagagt
    15061 ggcgagaaca tcggcaagct gtcggtggtg atggccgata acgaggccga agccagcttg
    15121 accgaggcgc tgcgcaagac gatgcaggcg catccgtcgg cgcaggtcga tttcagccgc
    15181 ccggaattgt tcagcctgtc gccgccgctg gaaatcgagg tggagggttc cgacctggag
    15241 ggcatccgcg tcgctggtgc caagctggcc gcgatgctgc gcgccaatcc gcactacgcc
    15301 gacgtgaagt cgacggtgga gcagggcttc cccgagatcc agatcgtgtt cgaccaggat
    15361 cgcgccgccg cgctgggcct gaccacccgc cagatcgccg acgtggtggt gaagaaggtg
    15421 cgcggcgaag tggccacccg ctacagcttc cgcgaccgca agatcgacgt gctggtgcgc
    15481 gcgcaggaaa gcgaccgcgc ttcggtcgac gacatccgcc gcctgatcgt caatccgggc
    15541 agcggcgcgc cggtggagct ggctgccgtg gccgaggtgg tctccaccac cggccccagc
    15601 gagatccatc gcgccgacca gcgccgggtg gcgatcatct ccgccaacct gcgcgacatc
    15661 gacctgggca acgcgatccg cgaagtcgaa gcgatggtcg cgaagacgcc gctgggcacc
    15721 gacatcgaca tgcgcatcgg tggccagggc caggagctcg gcgaatccat ccgctcgttg
    15781 ctgttcgcgt tcgccctggc gattttcctg gtgtacctgg tgatggcctc gcagttcgaa
    15841 tcgctgctgc atccgttcgt catcctgttc accatcccgc tggcactggt gggcgcggtc
    15901 gccgcgttgc tgctgacccg cgcaccggtg tcggtggtgg tgttcatcgg attgatcctg
    15961 ctggtcggcc tggtggtgaa gaacgcgatc atcctgatcg acaaggtcaa ccagttgcgc
    16021 gaggacggcg tggcgaagcg cgaggcgctg gtcgagggcg cgcgttcacg cctgcgcccg
    16081 atcatgatga ccacgctgtg cgcggtgttc ggcttcctgc cgctggcgct ggccttcggt
    16141 gaaggcgcgg aagtgcgctc gccgatggcg accacggtga tcggcggctt gctggtctcg
    16201 acgctgctga ccctgctggt gatcccggtc gtgtacgacc tgctggaccg caagcccgac
    16261 gagttctacg ccgagcgcgg ccggcgcgcg cgcaacgccg ccgcgcaggt cgcggaagtg
    16321 ctgtcgcacg agcacgacgc gctgggcgaa ggcgccgaca aggcctgatg cgatgaatat
    16381 caccgagctg tccatccgca ggcccgtcac cacgatcatg ctgttcgtgt cgatggtggt
    16441 gatcggcctg atcgcgtcgt tccggcttcc gctggaggca gaaccggaag ccaccatccc
    16501 gttcttcttc gtctcgctgc cgtattccgg ctcgaccccg gaggaagtgg agcgcaacct
    16561 ggtccgaccg gtcgaggaag cgctggcgac gatgcccggc atcgagtcga tgaactcgcg
    16621 cgcgaacgcc gagggtggat cggtgcaggt gcgcttcagc gactggaccc gcgacatcgc
    16681 catcgccgca tccgaggcgc gcgagcgcat cgacgcgatc cgcgacgacc tgccggacga
    16741 tttccgccgc tatttcgtct atcgctggag cacctccgat cgcgaggcga tcagcctccg
    16801 cctgaccagc caggtcgacc tgaccacccg cgccgacctc atcgaacgca gcatcaagca
    16861 acggctggaa cgcctgccgg gcgtggcccg cgtggaagtg gagggtgtcg ccaagcagga
    16921 agtgctggtg gcgctggaca aggaccgcct gaccgcgcac ggcgtggcac tgaacgagct
    16981 ggtgcagcaa ctgcagacgg cgaacttctc ggtgtccgcc ggacagatca ccgaggccgg
    17041 gcagcgcctg cgcgtgcagc ccaagggcga gttgctggag ctccagcaac tgcgcgacct
    17101 gcgcatcgac cggcgcggta cccgcctgtc cgacgtcgcc gacgtggagt tgaagccgca
    17161 gcggatggat tacgtgcggc gcatggacgg caagcccagc gtcggggtgg acatctacaa
    17221 ggagcgttcg gccaacctgg tcgacctgtc gcgtggcgtg cgcgcggaaa tcgccgagct
    17281 cgagaaagac ccggcgatgg gtgggatcaa catcgaagtc accgacgacc aggggctggc
    17341 ggtgaccagc tcgctgctgg cgctcgccga ggccggcggc atcggcctgc tgctgtcggt
    17401 gatcgtgctg tacgcgttcc tgcgccactg gccgtccacg ctcatggtga gcacggcgat
    17461 cccgatctgc ttcgtcatga ccctgggctt catgtacttc accggcatct cgctcaacat
    17521 catctcgatg atgggcctgc tgctggcggt gggcatgctg gtcgacaacg cggtggtggt
    17581 ggtcgagagc atctaccagg agcgcgagaa gtatcccgac cggccctggc tggcgtcgat
    17641 catcggcacc cgccacgtcg ccatcgcgct gtcggcgggc accctgtgcc actgcgtggt
    17701 gttcctgccg atgatgttcg gcgagaagaa catcatcacc atctacctgg gccaactggc
    17761 ggcgaccatt tcgttctcgc tgctggcatc gtggctggtg gcgatcagcc tgatcccgat
    17821 gctgtccgcg cggatgaaga ccccgccggc gctgaaatcg ccgttcatca ccgcgatgca
    17881 ggcgcgctat gcgcgtgcct tgcgctggac cctggggcat cgtggctgga gcgtggccgg
    17941 catcctcgcc atctccctgc tcagcatcta tccgatgacc catacgcagg gtggcggcga
    18001 cgacaacgac cccagcgaga tcaacatctt ctaccagtgg aagggcgcgt attccaagga
    18061 agagatgggc aaggaagtgg cgcgggtgga ggcgttcgtc aacgccaacc ggaagccctt
    18121 ccacgtcgag cgcgtgtaca gccgctacag cgagcagggc tgggcgcaga cgcgcctgtt
    18181 cctggacatc gaggatccgg agcaaagcaa gaagatcgcc gaggacgtgc gcaaggggct
    18241 gccgaaatcg gcgcgcgcca acctgggcat cggctggccg ggcgggcagg gcaacgaggg
    18301 cgagggcatc accttcagcc tgatcggcga ttccacccag accctgcagg agctggcggg
    18361 cgacatcctg ccgatgctgg cgcgcaaccc gaagctgcgc gacgtgcgca tcgacaccgg
    18421 cgacgccaac accgaactgc gggtgcaggt gaaccgcgaa cgcgcggcgg cctacggctt
    18481 cagcgcgcgg gaagtggcgc agttcgtcgg cgtcgcgctg cgcggatcct cgctgcgcga
    18541 cttccaccgc gaggacgtcg agatcccggt caacgtgcgc ttcgccggct ccgacgacta
    18601 cggcatggaa gacctgtcca ccttcatggt ccgcgcaccg aacggcagcg acgtgccgct
    18661 gctggcgatg gtcgacgtcg gcacgtcgcc ggccgccacc tcgatccagc gacagagccg
    18721 gcagaccatg ctgcaggtgc aggcgggact ggccaaggac gtttcggtcg aggacgcgcg
    18781 caaggcgatc gagaccaccc tcaacggcac gcagttcccg ccgggctaca gctacacctt
    18841 cgacggcgcc ggcttcaaca tcaacttcga cggcatggac cagatgatgc gcgcgatcat
    18901 gatcgcgctg gtgctgatgc tggtgatcat ggccgcggtg ttcgagtcgc tgctgttccc
    18961 gctggcgatc atgtcctgcg tgctgttctc gatcttcggc gtgtactggc tgttctggtt
    19021 gaccggcacc gagatgaaca tcatggcggt gatcggcatc ctggtgctga tgggcgtggt
    19081 ggtgaacaac ggcatcgtga tgatcgagca catcaacaac ctgcggcgac gcggcctgcc
    19141 gcgcaccgag gcgctgatcg aaggcagccg cgaacgcttg cgtccgatca tgatgacgat
    19201 gggcaccgcg atcctggcga tgatcccgat cgcgctgtcc accaagaccg ccgagggcat
    19261 gccgccgtac tacccgatgg cgcgcgcgat cgccggtggc ctggcgttct ccacggtggt
    19321 cagcctgctg ttcctgccga ccatctacgc gatgctggac gacctgcgca tgggcagcgg
    19381 caacctggtc cgcagggcgc ggatgaaccg cgcgcagcgg cgtgacgcga aggccgctgc
    19441 cgcgtgagta cgggcggcga gcttcgcggg gacgggcgtc tcgccggcgc cctctacctg
    19501 atcgtcgtgc tggccggcat gcactgcctg gcctatgtgc cgggacaggt tggcgaaacg
    19561 ctcgcgcaag cctcggcgca tgccgggatg ttccgcagcg gcatcgccag tttcctggtg
    19621 atgcaggtcg cgttcctgct gctgccgttg gcgctgtacc gggtgctggg cgatgtcgac
    19681 cgccgtgccg ccatcgcgat ggtggcgctg gcggtggtca gcgtgccgat cgggttggtc
    19741 gccgtcaccc accgcatgga agcgttggcg ctgctcgacc aggcggcagc aggcaccacc
    19801 gatgcgatgg aggccgcctt cgcgctgagc ctgcagcgtt acggccatgg cctgcgcgtc
    19861 gccagcctgt tctggggatt gtggttgctg ccattcggct ggctggtgct gcgttccggc
    19921 cggttgccgc gcgtgcttgg cctgctgctg gtgctgggtg gcctgggcta cgtggctcag
    19981 gtgttcggcg gcctggtgcc cggtctcgcg gaggcgtcct ggctgcgcta cgtgcgcatg
    20041 cccgccgcgt tcggcgagat cggcagctgc ctgtggttgc tggtgttcgg cgcgcgtgca
    20101 ggccgagtcg accccgtgcg aacggcggcc tcgtgaccat cccggtgtct ccgtcgcgcg
    20161 agctcgccga cccgccgctg ccaacgcggc tgaagctgtc cgcgttgtgg gccagcctga
    20221 ccttctgcta cctctacggc gactacttcg gcctgtacaa gcccggcaag ctccagcaca
    20281 tgctggaggg tgccgggccg atggggccga ccagccaggg ctcgttgctg ttcgtcgccc
    20341 tgttgctggt ggtgccgggg ttgatggtgt tcctgtcgct ggcgctgcca gcgcggccgg
    20401 tgcgctggct caacatcggc ctggcggtct tctacgccgc gttcgttgcg atcacgatgc
    20461 ccggcgcgtg gtggttctac ctggcctaca gcagcatcga aatcctgctg ggcgccgtca
    20521 tcgtgtggct agcctggcgc tggccacgca cggcctgatc gggttcatcc catcagcttg
    20581 ccgatgatcg cggcgccgga gacccacacg ccgatcatgc tgccgatgtt ggtgagcagg
    20641 aacaccagca ccacccggca cacgcggttg cgataccagc cgccgagcgt ctgcgcgtcg
    20701 tcgcgcaggg ccaggaaatc cggataggcc ggcttgcgca ggtgcacttc gaccagcgca
    20761 ctgaacatgc ccggcggcag gccggggcgg aacggcttca gcggcgcgga gatcgccgcg
    20821 gccaggatgc tgaggacgtg gccgcgtgcc agcaatgcgc ccagccccgc gagtccgccg
    20881 gtccacacga cccattgcag caagagttcg cggcccagct cgcggccgcc attgaaatag
    20941 ccccaggcga tgccgccgca gatcagcaac atcaggatga tcgtgatcca cgggatgttg
    21001 cgcttcttga ccacgtgcgc cagttgcgcg gtgagttcgg ccggttcgcg ctggtcttcg
    21061 gtcaggtagc gcgccatgcc cttgaggtgg ccggcgccca ccacggccag cacgtgcgtc
    21121 gcgccgtcgc tgcgttcgcg cagcttggct gccatgtact ggtcgcgttc gtcgatcagg
    21181 gtcgcataga gcgagggcgt gtcgcgcgcg aattcgccga agctcgactc gagcatgtcg
    21241 ccttccttga gccgctcgat gtcgtcctcg gacacctcgt cgctggcgaa cagtccgccc
    21301 gccaggccac cgaccagctt catccggtcc caccagctca gcccctgcag gatgcggcgg
    21361 aaggtgatgc cgacatcgcg gtcgatcagt tgcaacgaca gcccgcgcgc ggcggcctcg
    21421 accgcggccg ccttgagctc cgcgccgggt tcgatgccga gctgctcggc caggcggcgc
    21481 tggtaggcgg ccagtgccag gttcgccgcg aagggcgcga tcttgcgctc gcgaatgacc
    21541 ttgaccaggt cgagttgcgc cagcgcatcc ggatgggtga gcgccttgtg ccgctgctcg
    21601 tccagctcca cggcgaccgc atcgaagcgt cccgtgtcga tcgccgccat gaccgcatcg
    21661 atgctggtcc tggagacatg cgcggtgccg agcagggtgt agtgcacgcc gtcgcgctcg
    21721 agttcgacga cgggctgccc ggcgaaggcg tcggcggcgc tgttggattc ggtggtcatc
    21781 gtgcctgcgg gttgtccggg aagccgccat tcgcatggcg gcggcgcagg atagacggtt
    21841 tcgagctggc gaagcgtgtg cgttcaggcc agcccgtagg gcaggtcggc ggtggagacc
    21901 acgcgttcgc cgatggactc gccgcgcagg aaacgcagcg ccgaggcgat cacgccctcg
    21961 tcgtcggcga tgcggttgtg gccaagcccg cgtgtgctca gcaaccgcga ttgcggccag
    22021 tagcgcgccc agcgttcgcc ctccgaccac ggcacctcgc gatcctcgat gtcatgcacg
    22081 atcaaggccg ggcggccgat gcgcggtgcg ttgtgctgtg cctgcaattc atccgcggag
    22141 aatcgcatcg cgccttcgaa gcgcgcgaac atgcgccggc ccaggtggct ggcaagccac
    22201 agcaggttgg agaaccgttc gaccgcggcc agcggatccg cggcgggcgc gatcaggacc
    22261 gcgcgcttcg cctgcatgcc gcgggccagg gccagcgcgg tcgccgcacc gcccagggag
    22321 tggccgatga cggccgccgc agggccgtag tgtttcgcga ctgccatcag gtgacaggtg
    22381 aagtcgggca gggtggtgtg gatgccaccg ctgcggccgt gtgcggcctg gtcgaaggtg
    22441 accaccgcgt atccggcgtc ccgcagcggc tggatccagc gcgccacgcg ggtgccgtgg
    22501 cttgaccagc cgtgcgcgaa gaggatgtag ggctgcgtgg ccggatcgcc ccaggcgtag
    22561 gtatgcaggg tgatgccatc gataccgagg tcgtcctcac gggcgccgag cgtgggtgcc
    22621 gcgcgtgccc gcgagcggct cgaggcgaac ggcgtggtga acaggcgggc ggccttcgcc
    22681 accatggctt cgggcgcaat gggcccgccc agcgcgaaga tcagccgcac cagcggcagt
    22741 ttcagataat cacgaacggt cgtgctaatt gcgcagagac tggcaggcat gacggcacct
    22801 cgtggcggga tcagggggag ggagcgtggg cggcgaacca gcgttccagc gcgctgtcgc
    22861 cgtggcggcg tgcctggtcg taaccgaaca ggccggcttc atgcagcatc gccaggggaa
    22921 ttgcgtacag ctcgaaggcg tactggtcgg tgtcgccgtc gcgcaggtgg ccgcaatcga
    22981 tcgccagctg cgcggcgcgc tgcagggcct ggcgccaacg ccgttcgttg aggacgacct
    23041 ggtcgcggag tggtccggga cggtcgtcga attcgctgac ggaggccacc agtacgcaac
    23101 caccggtttc caggcgtccc cactcgaacc agttgcgcat catcgcgcgc agtcgcggca
    23161 aaccacgcgg ctgggacagc gcggcaccca gcacgaattc gccaaagcgc tggcctgccg
    23221 attccagtac ggcgagttgg aggtcttcgc gcgatccgaa gtgggcgaac acgccactct
    23281 tggacatgcc cactgcttgc gccaggggac cgatcgacag gccctcgata ccggcgaagc
    23341 gggcgatctc gtaggctttt tcgatgatcg cgtcgcgggt ggcggcgccc ttgctggtgg
    23401 cggtcagcgg attcatggtc gagacattag cacgaccgtt cggatttatt tgaccgtctg
    23461 tcggaatagc cttggcgagc gatcagtccc gatagcgctt ggtcaggtcg ccataggcat
    23521 cgatgcggcg gtcgcgcagg aatggccaga tccggcgcac gtgttcgctg cgggccatgt
    23581 cgacctcggc caacaggatc gtcggcccgg tgccggcctc ggcgatgaat tcgccctgcg
    23641 gcccgagcac gtggctgttg ccccagaagt cgatgccgga tgcgcccagc ggcgacggct
    23701 cgtggccgac gcggttgcaa ctcagcaccg gcaggccgtt ggcgaccgca tgaccgcggt
    23761 ggctcagcac ccacgcatcg cgctggcggg tcttttcggc cgagtcgtcc gccggatccc
    23821 agccgatcgc ggtcgggtac agcagcagct cggcaccggc cagcgccatc agccgtgcgg
    23881 cttccggata ccactggtcc cagcacacca gcacgcccag gcgtcccacg ctggtgtcga
    23941 tcggggtgaa gcccaggtcg cccggcgtga agtagaactt ctcgtagaag cctgggtcgt
    24001 cggggatgtg catcttccgg tacttgccgg cggtgctgcc gtcggcatcg aagaccaccg
    24061 cggtgttgtg gtaaagaccc gcggcacgct tctcgaacag cgagctcacc agcaccacgc
    24121 cgtgttgctt cgccagcgcc gccaggcgtt ccgtgctggg ccctgggatg cgttcggcca
    24181 ggtcgaactc gtccaccgat tcgtgctggc agaagtacgc accgttgtgc agttcctgca
    24241 gcaggacgag tttggcgccg gacttcgcgg cctcggacac gcgggcctcg atgactgcga
    24301 ggttcgctgc ggcatcaccg tggttgcgtt cctggatcag cgcgacgggc agcgtgttgt
    24361 acttggacat cagaccagac cctcgggcaa ttgcatggtg atgcagtgca ggctgccgtt
    24421 ctgccagatc agcgggcggc acgggacctg cacgatctct cgatcgggga acgcgtcggc
    24481 cagcaccgcc gcagcggcat tgtcggcggg atcgccatac gccggcatca gcaccgcgcc
    24541 gttgacgatc aggaaattcg catagctcgc ggccaggcgc cggcggctgc catcggtcgc
    24601 gatgtcgatg accggcttcg cccacggcag gggaaacagc cgatacggcc ggtcctcccg
    24661 ggtgcgcaac gcggcgatct cctcgcccat cgccttgagg tcggcgtaat gggaatccgc
    24721 cggatcgtcg caggcctgga acacgatggc atcgcgcccg gcaaagcggg cgagggtgtc
    24781 gatgtgggcg tcggtatcgt cgccctccag tgcgccgtgg tcgagccaga gcacccgatc
    24841 ctgccgcaac cagccggcga gcctggtggt gagttcttcg cgcgacgcat cggggtggcg
    24901 ctcgtgcagg cactgccagg tggtcagcag ggtgccgtcg ccgtccgtgt cgatggcgcc
    24961 gccttccagt gcgaagtcga cttcgtcgcg cgcagcatcg ccgaacagtc cgttcgcatg
    25021 cagacgctgg accagttggt cgtcgtcgct ggccccgtac ttgccgcccc agccggtgaa
    25081 gcggaaatcg agcaggcgga acgcgtcgcc atcgcgcaag gtgatcgggc cggaatcgcg
    25141 cagccaggta tcgtcgtagg gcacggtggc gaagcggacg cgatccatgt ccacgcggtt
    25201 gctgcgcagg cgcatctccg cgtaggtctc gatgtcgtcg tcggccacgc agatcaccac
    25261 gcgctggaag cgcacgatgg ccgcgaccag cgcgatgtac gtctcttcga cctcggccag
    25321 gcgttcggcc cagtcggtct cggcattcgg ccacgcgatc aggatcgccg cctggggttc
    25381 ccactcggcg gggaaacgca cgatgtcttg catgctggct cttaggtggt gggcggcttc
    25441 ggcccgatct cgttcgggtc ggccttggtc atcaccacgt cgatgacctt gctgcgctcg
    25501 aaatacacgg tgaaggccgg ataggtccag cggttgatcg tcggccactg gcgcttctgc
    25561 ccgccgcgcg gatccaggcg atcactcggt gcgccgaagc gggcttcgac ctgcgacatg
    25621 gacatgccac ggctgggcag gttgcccggc tcttcctgca cgcgttcgat cagcaggacg
    25681 tcgccctgca cctgggcctg ctgggccagg gcgatggcgg gggcgcagac gagcgtggcg
    25741 gcgaaaacgg acaagatgct gcgggacaga cggttcatgg cgacgctccc ggttgcagga
    25801 tggtgacgac ggattctatg cccgaatcgt gaacgggccg cgatggcgtg gccttggcaa
    25861 cgccggacag cagaaagccg ccttgcggcg gctttcgaca gtgcaacccg ggcaatcggc
    25921 ccagcgcttg cgcgccctgg ccgtgccggt aaggctcctc agcgctggcg cgccttgaac
    25981 cgcgggttgg acttgcagat cacgaagacc ttgccacgac ggcgaaccac cttgcagtcg
    26041 cggtgacggt tcttcgccga cttgagggag gacaggacct tcatgacaaa acctcggcaa
    26101 aaatgatgga tggaacggtt ggaggaagcg caacggcacg cccggagagg ctgcttgcga
    26161 agcgaagccg cgcattctaa cggggtttgt gtatccggat caacagcttg aggcgcattc
    26221 ctgccggatc ggacggaatc ggccgtcggc acggtcgcgc cggcataatc gcggcatgga
    26281 tcccaacgcc cccgtactca ccatcgatgg tccctccggt tccggcaagg gcaccatcag
    26341 ccgcatcgtc gcccagcgcc tgggctggca ttacctcgat tccggtgcac tttaccgcgc
    26401 cgtgggcgtg gcggccggct gggccgacct ggacctggac gatgccggcg ccctggtgcg
    26461 ctgcaccttc gacaccctgg tcggcttccg cgagggggat gccggtgacc tgcgggtgct
    26521 ggtcaacggt cacgatgcaa cggacgaact gcgcaccgaa accgcgggcg cggcggcctc
    26581 ggcgatcgcg gccatcccgg aggtccgggc cgcattgaag gatcgccagc gcgcgttccg
    26641 gcaattgccg gggctggtgg ccgacggccg cgacatgggc acggtgatct ttcccgatgc
    26701 cggccacaag gtgttcctga ccgcgagcgc cgacgagcgt gccgagaggc gctataagca
    26761 gttgaaagac aaaggggttt ccgtcacact cgacggtctg ctgcgcgaga ttctcgcccg
    26821 cgatgcccgc gatgccagtc gcgcggtggc gccattgcgg ccggccgacg acgccgtccg
    26881 catcgacacc accgggcttg gcatcgatgg cgtggtcgcg ctggtgctgg acgtggtgtc
    26941 ccgcccccgc gcgcactgag cgccccgcac ggcagaaccc ttccacgccc gcatcccgcg
    27001 tgcgtggcgt ccatcccacc atcacacatg gcggagcgcg tcctgcgcga tgccagaccc
    27061 caggtgggtc gccccgtacg tgtgggcgac ctgtgtatcc acccgagatt tttcatgaat
    27121 tccgtagcta ccgaatcctt cgccgaactg ttcgagcaga gccagaccaa ctgggccaag
    27181 atcaagccgg gcgccatcgt caagggcatc gtggtcgacg tccgcaacga cgtcgtggtg
    27241 atcaacgccg gcctgaagtc cgagggcatc gtcccgatcg a
//
LOCUS       DAOOCX010000018        26990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1451_length_26990_cov_8.212549, whole genome shotgun sequence.
ACCESSION   DAOOCX010000018 DAOOCX010000000
VERSION     DAOOCX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 26990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26990
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1451_length_26990_cov_8.212549"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..104
                     /locus_tag="PK399_03095"
     CDS             <1..104
                     /locus_tag="PK399_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HPW12138.1"
                     /translation="NEDAMATDKLADGIRKFAVDQRKLESLLAAKLG"
     gene            116..1405
                     /locus_tag="PK399_03100"
     CDS             116..1405
                     /locus_tag="PK399_03100"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW12139.1"
                     /translation="MRERRPHVVIVGGGFAGLWATRALGSAKVRITLVDRGNHHLFQP
                     LLYQVATAGLSAPDIAAPLRHILRRQRNAEVRMATVTGIDADAKCIALDDGTSLGFDS
                     LLLASGATHAYFGNDHWAGHAPGLKTLDDALDLRRKLLLAFERAEATSDPAERDAWLH
                     FAIVGGGPTGVELAGTLAEIARHTLKDEFRNIDPAQARVRLVEAGPRVLAAFPEDLSA
                     KAKRQLEKLGVEVSTGTPVKDINADGYTLGDTVVPAKTVVWAAGVAASPLGAMLGAPL
                     DRAGRVQVQPDLSVPGHPDIFVAGDLAALSQANGKPVPGVAPAAKQMGNLVARNIRAR
                     LSGHPTTLFAYRDYGNLATIGRMAAVVDLGKLKFSGLLAWWFWLVMHVFFLIGFRNRL
                     VVLLNWWWAYWSYQRGARIILGGDADDVDQARTTRQA"
     gene            complement(1378..1845)
                     /locus_tag="PK399_03105"
     CDS             complement(1378..1845)
                     /locus_tag="PK399_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HPW12140.1"
                     /translation="MTFRILALPVAPFAADFAADDKALRARGIRRVVADDKPHYPCRV
                     SLQDAVEGERLLLVPYLHHGVDTPYRASGPIYVREAAVQARPTVGEVPALLRSRLLSL
                     RGYDARGMMAWADVVPGSDVESGIAALFAIERVAYLHLHYAKPGCYACRVVRA"
     gene            1976..2599
                     /locus_tag="PK399_03110"
     CDS             1976..2599
                     /locus_tag="PK399_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007971319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HPW12141.1"
                     /translation="MAFALQLFDLVGIFVFALSGGVLAVRHRLDLFGVLVLACATAVT
                     GGIVRDVLIGAVPPAALADWTLLSTAMLAGVVTFCWHGIIERLRNPVLMFDAAGLALF
                     AVLGTGKALAFGLSPFAATLLGIITGIGGGIARDLLVVRTPVVLQQTELYAVAALAGG
                     GIVAIAHALGWPQAPAMVVGALLCFGLRFMAIRRGWRLPVARDADQA"
     gene            complement(2663..3544)
                     /gene="rpoH"
                     /locus_tag="PK399_03115"
     CDS             complement(2663..3544)
                     /gene="rpoH"
                     /locus_tag="PK399_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002803651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HPW12142.1"
                     /translation="MNGATMSTALVANNLPIPSAMGSLEAYIGAVHQIPVLSVEDEQR
                     LARRLREDNDIAAAQELILSHLRFVIHVARGYSGYGLQIGDLIQEGNIGLMKAVKRFD
                     PTVGVRLVSFAVHWIRAEMHEFIIKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNAD
                     EVRAVAKDLNVSEREVLEMESRLSGRDVGFDAPDDADDDNAPPAPVNYLVASNDIDPA
                     MAYESANDEDNQLELLREGMARLDARSRDIIKRRWLDADSKVTLQELADEYGVSAERI
                     RQVEANALKKMKALFAA"
     gene            complement(3715..5748)
                     /locus_tag="PK399_03120"
     CDS             complement(3715..5748)
                     /locus_tag="PK399_03120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPW12143.1"
                     /translation="MDNPLLDFSGLPRFDAIRAEHVTPAIDSLLAEAEAAVRHAQTVS
                     PVTWASFVTPLEDATERLWRAWGQVAHLQAVVDTPELREAYNANLPKLTRFGAALGQD
                     LALFAQYRALAASPDYAQCDATRRKAVDNALRDFRLGGAELDDAGKARFAAIQEELSA
                     LSATFSQNVLDATDAFTLHVDDEARLSGLPADVVATARVTAEKAGKPGWTLTLKMPCY
                     LPVQTYADDRGLREALYRASGVRASEHGPPTLDNSGHIDRILALRTELAALLGFASYA
                     DYSLATKMAQSPAEVLGFLRDLAARALPFARRDRTELEAFARDELGIDDLQPWDLAYA
                     SDKLKQARYGYSGQEVKRYFTEPTVLAGLFDVIQSLYGVRVRQDSAPTWHPDVRFFRV
                     EDSAGTLLGQFYLDLYARDGKRGGAWMDDGRNRRSTAAGTQTPLVHLVCNFGKGVDGQ
                     PATFSHDEVTTLFHEMGHGLHQLLTAVDVLPVAGINGVEWDAVELPSQFMENFCWEWA
                     RVQAMTAHVDTGEPLPRDLFERMLAARNFQSGMATVRQLEFALFDMAIHGDFDPADDS
                     VAALLERIRDEVAVNRPPAWHRFPQQFSHVFAGGYAAGYYSYKWAEVLSADAYAAFEE
                     APARLAETGARFLAEVLSRGGSRTALENFTAFRGREPSIDALLRHAGMAAPAA"
     gene            complement(5855..6565)
                     /gene="ung"
                     /locus_tag="PK399_03125"
     CDS             complement(5855..6565)
                     /gene="ung"
                     /locus_tag="PK399_03125"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007971317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPW12144.1"
                     /translation="MADIRIEPSWKSRIGDWFAREDMQQLSAFLRQRKAAGATIYPAG
                     ASIFAAFDATPFDAVKVVILGQDPYHGPGQAHGLCFSVLPGVDVPPSLQNIYKELASD
                     VGFARPDHGCLLPWAQRGVLLLNSVLTVEAGQPGSHQGKGWEGFTDHVVDVLNRERDG
                     LVFLLWGSYAQAKGKVIDARRHRVLKAPHPSPLSAHRGFLGCGHFSAANEYLTRNGQA
                     PVDWALPSRAALALSASR"
     gene            complement(6584..7423)
                     /locus_tag="PK399_03130"
     CDS             complement(6584..7423)
                     /locus_tag="PK399_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW12145.1"
                     /translation="MANDLRHVVSDAPRVMVVDGSKLVRKLIGDTLLRELPGTEVIGC
                     AGIAEARETLAKGAVDLVTTALVLPDGDGLALARSVREAQGQAYVPVIVVSGNAQEML
                     ESRAFTEDVTDYFDKSLGHGALAAFIRGYVQPAPIEGARVLYVEDSRVVAVATKRMLE
                     RQNMQVLHVLSVEDALEHLHRFRGRTDGDVGADLVLSDVYLKGILNGHDLLAQIRTAF
                     GYGKRRLPMVMMTGDDNRDNQASLLRDGANDLVLKPIEERLLVTKVLFQLRLAKLPEP
                     GAQ"
     gene            complement(7425..8378)
                     /gene="ftsX"
                     /locus_tag="PK399_03135"
     CDS             complement(7425..8378)
                     /gene="ftsX"
                     /locus_tag="PK399_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPW12146.1"
                     /translation="MNDQASPNVQASRFGAWLDHHGFSIVASLGRMLRKPWATLLTVG
                     VMAVALALPLGLWVVLQNLQRLGGEVQSSRDVAVFLKPEIDATRAQAIADTLRARGDV
                     AKVDLIAPEQALQQLRARDDLAAAIDALGEDAARAALPSVLRVSPRGDELLLAESLKA
                     LPDAERVQHDAVWRERLDAWLRFGGRAVLVLAALLGLGALLVVGNTVRLDIQSRREEI
                     GVLQLLGASDGFVRRPFLYLGAWYGLGAGALALGVLTLAWLALREPLADLAARYGSNF
                     ALQGLPPLPALAVLAAAGVLGWLGAGLVTGHYLRQTRPVER"
     gene            complement(8371..9060)
                     /gene="ftsE"
                     /locus_tag="PK399_03140"
     CDS             complement(8371..9060)
                     /gene="ftsE"
                     /locus_tag="PK399_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HPW12147.1"
                     /translation="MTVLRFDSVSKQYPGGHTALSEVSFAVDAGEMLFVTGHSGAGKS
                     TLLKLIQLAERPSRGAVLFGDRNLLKVRGGAIARHRRSIGVVYQNHQLLMDRSVAENV
                     ALPLVLRGMKRAETGKRVRSILERLGLGARDRALPSQLSAGEQQRVGIARAVVAEPTL
                     LIADEPTGNLDPTLSAEIMALFASLPERGTSVLVASHDLGLVKRMKKRVLVLDHGQLL
                     DDISPEDLADE"
     gene            complement(9134..10843)
                     /gene="rhlB"
                     /locus_tag="PK399_03145"
     CDS             complement(9134..10843)
                     /gene="rhlB"
                     /locus_tag="PK399_03145"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HPW12148.1"
                     /translation="MSDKPLTDVTFSSFDLHPALIAGLEAAGFTRCTPIQALTLPLAL
                     NGRDVAGQAQTGTGKTLAFLVAVVDRLLKRPALADRKAEDPRALILAPTRELAIQIHK
                     DAVKLGDQLGLKFALVYGGVDYDKQRELLQRGADVIIATPGRLIDYVKQHKVVSLHAC
                     EMCVLDEADRMFDLGFIKDIRFLLRRMPERTTRQTLLFSATLSHRVLELAYEHMNEPE
                     KLVVETEFITAAKVRQKLYYPADEEKLPLLIGLLSRSEGARTMVFVNTKAFVERVARG
                     LERAGYRVGVLSGDVPQKKRESLLKKFQAGQLEILVATDVAARGLHIDGVSHVYNYDL
                     PFDAEDYVHRIGRTARLGAEGDAISFACERYGMSLPDIEAYIDQKIPGEHVTPEMLVA
                     LPRKPREGVEANAEDGESIGEIFKEVREARAAEDAKRGGGRGGPRSGGARSGERSSSA
                     PRSGDRPARGPRPPRKPRVEGDVAAAATVTGTVVADAAVPATAALAQPAAPSTGIEDD
                     ARKPRKRRRRRHGRPLEGEAGTTPNAPLPAGDKTAPAPAAGGDKPSLFSRIGRRIKSL
                     VGG"
     gene            11061..11387
                     /gene="trxA"
                     /locus_tag="PK399_03150"
     CDS             11061..11387
                     /gene="trxA"
                     /locus_tag="PK399_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPW12149.1"
                     /translation="MSDTVLHATDADFDHQVLASDQPVLVDFWAPWCGPCKMIAPALD
                     DLAGTYAGRAKIVKVDVDQNQATALKYHVRSIPMLLLFKGGQVQATQIGAVGKAQLAQ
                     MIEKSL"
     gene            11654..>11899
                     /locus_tag="PK399_03155"
     CDS             11654..>11899
                     /locus_tag="PK399_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12150.1"
                     /translation="MSDPTNDAGEPAEKRVRKTRVAKTAAPKDSLTIPPLEAASAAPP
                     AAPAATPAPPPAVAPPSAPSAPAPATASHATHETPAPS"
     assembly_gap    11900..11999
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12000..13590
                     /gene="rho"
                     /locus_tag="PK399_03160"
     CDS             <12000..13590
                     /gene="rho"
                     /locus_tag="PK399_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPW12151.1"
                     /translation="APAPATASHATHETPAPSATATGSTDGSDAGGNAQQHGQQGQHQ
                     NRHNHPNNQNGEAGQQGNRRDRFKNRRDRNRDRPRDQQGFPDDNGGGNGEGFVPRPHP
                     QVPEGFPTYSLGDLKRMPAHKLLEIADQLQISEGVARARKQDIIFGILKVLTRHGEGV
                     AADGVLEILPDGFGFLRAAEASYLAGPDDTYISPSQIRRFNLRTGDHISGRIRWPKDG
                     ERYFALNIVDTINGEPLEASKNKALFENLTPLFPRKKFKLERGDGSSEDITGRILDLM
                     APQGKGQRALIVSPPKAGKTMMMQQIASAISYNHPDVHLIVLLIDERPEEVTEMQRTV
                     RGEVISSTFDEPAARHVQVAEMVIERAKRLVEHKKDVVILLDSITRLARAYNNVLPSS
                     GKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSAMDKVIYEEFKGTG
                     NSEVHLDRRITEKRVYPAIGVNASGTRREDYLIEPDLLQKIWILRKLLHPMDEIAAME
                     FLLDKMKSTKSNDEFFGSMKR"
     gene            complement(13665..14519)
                     /locus_tag="PK399_03165"
     CDS             complement(13665..14519)
                     /locus_tag="PK399_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12152.1"
                     /translation="MRSIVQIGFGAALLCAALVATAADRGSGHFRKGEVRLEAKHVIA
                     VTVDEDGDAASAQTYVYLSSVPMDARKVASAFQPSSAAEEQLGDGSAGYVRICINAEG
                     AECGLYFSHNNPSASFNSSGYGVFKLDPAPKGRIAGRWVLAEPDDFFGETYDFDLTFD
                     VAITPPPGTALPADGGEPGKAYRAWTTAVAKGDLAVLRRLVGGDYNAWRIKSDDESDV
                     KAALKDLRDGTPVQARVLRGRIDGDDAVLWVEGKDRDDILRRGRVSLTRADGEWRYME
                     ADLDSVSD"
     gene            complement(14602..15381)
                     /locus_tag="PK399_03170"
     CDS             complement(14602..15381)
                     /locus_tag="PK399_03170"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW12153.1"
                     /translation="MKTPMLCVAIACMAASPAIAADDPAKPDVVAFNASVRVEVDAAG
                     KPVKVEAPADLPVAIRAFIEKRVASWQYEPAKVDGVPASAVTFVKVGACAIPAAEGYR
                     LGLDFKGNGPALVSQGPWFIPPPDYPFELRRRGAEGTFRVTYSIQADGTTRLASIEPM
                     EGTLGRFVKPFRATLTSWIEGMRYQPEQVGGRPVSTEMRFPVSFMLSSAKSGPRSNDE
                     YREQLEARALESKECVAASGPGGPLPIATNSPVKVTPIPAG"
     gene            complement(15384..16256)
                     /locus_tag="PK399_03175"
     CDS             complement(15384..16256)
                     /locus_tag="PK399_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW12154.1"
                     /translation="MKMRLMAAIALLGGLLLAMPALSGSRGAAAVRKQAESSLQVSGT
                     ITIGKDGSVIAHTLDPEAPLGDALTAFVGKAVAGWRFQPVLVDGAPVNAKVPMHLRLV
                     AKRAEGDQVSVAIASSYFGSTDAVPKTDNPAGIKLSPPQFPKSALRMGGQGTVYLVVQ
                     VGRDGKVANVAAEQVNLRVAGTANEMDSLRDQFTMAALRVARGWSFAVPTTGEGADDD
                     SWLVRVPVEFVLGRPSDRRRNEGGWDTYIPGPRNTDMPWAQEKLKTAGSPDALPDNGV
                     YPLRQGATLVTPLG"
     gene            16349..18145
                     /locus_tag="PK399_03180"
     CDS             16349..18145
                     /locus_tag="PK399_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HPW12155.1"
                     /translation="MHGEGLELALVFLLAAVVAVPVFKRFGLGAVLGYLVAGALLGPH
                     ALRLVRNPEPVLAASEIGVVMLLFVIGLELSLPRLKVMRRPIFGVGGLQVLLSGLALG
                     ALAMSQDLGWKAALVVGLGLALSSTAVGLQLLSERKELTAEHGRLAFAILLFQDLAAI
                     PLLAAIPLLGKAGGEGLGWEDVAKAVLAIAALVLGGRYLLRHLFRIIARTGMPEVFTG
                     AALLVVLGSAWVMQLAGLSAGLGAFLAGVLLAESEYRHELEAQIKPFEGLLLGLFFMA
                     VGMTIDLQQIASEPLVIAAWVAALLAVKFALLFALGLRPGKLDLRGAMLLGGVLALGG
                     EFAFVVFAEAMKAGLLVEPLRDRLVAVVGLSMAATPLLLLLISRVAPAVEKRRRRAYD
                     DIPDEHPQVLIAGFGRFGQIVARLLWAQKIPFIAIDPDDEQVDFFRRFGNQVYYGDPT
                     HPELLRSAGAARVRVFVVAIDDVDKATTLVRLLRKHYPDARVLARARDRRHAWQLLDL
                     GVKPFRELFGSSLEMGREVLVALGMEARQATEYASRFREFDERVLDAQRLMQDDEDAL
                     LQSARNARRELEELFEADAGEGTLGRIVERKD"
     gene            complement(18171..21374)
                     /locus_tag="PK399_03185"
     CDS             complement(18171..21374)
                     /locus_tag="PK399_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPW12156.1"
                     /translation="MALGIAFLRGTRKRARLAADAGSLALLWGGGFVLAGVLVAWPLQ
                     HVLAAPALLPVLALSAAAGLLLVLLWWHWPLWHGSEREGGRLGLRFARQHLQDRQAWR
                     ALVQVALPVFLLLVGGLLLAWPGLLAGQVRIVATAVYAGLLPIAHLLLQAAPTLSLAH
                     GLPVVEMDAVEAEADDLVADLDDAPELALPLATVPVVDTVALGRELYAAARNGRVEKA
                     LQLLEAGADPNLPPEVGARDRRGLPVLAAVLPDLRLLRALIERGADVNLMLAGMNPLL
                     AATRDSWHGRPEAVMTLLANGADPRATDADGNTPLHHAARSSDPGVAALLRDAGAEMD
                     ALNIEAVSPLGTACSAGNWRLARFLLERGAKTEPVGGQPVLLAAAGTEEDDAAGVQLL
                     LRHKARVDARDARGRTALHEAAFAGHAGICEALLDAGADANVRDGEGRTPLLDATRAG
                     ALPALEVLVAAKADVHASDGQGANALHLACIAEAPSATMVRRLLELGVDPRAPDAQGQ
                     GAIDLASAAGRWTLVAALDPQRALPVSMDEDDGAPDRPPPLLLRERLLDEAGENQLAT
                     LLPLLARGDLDGLLCDADVAADPARVAWLLARGANPEARVRSAPMPVAAALARGTAGI
                     DNARLLFARGASPAGAGGLAVYLAACLTEGFTDARGEGFALELLERGADPYATSPAGD
                     PPVALAVRLHWPRLLQRLVAHGVDLDARDSHGMSALHLAAALGRLDELKQLLAAGAAP
                     DRRAADGQTPLGVALAAGRRDLAGWLDWRGWKLPRRPLQPLDVPAAAIVGDLDAVRRL
                     LDLGLPVDATDSQGCSALLRAAGGGHRALVELLLERGADPTLAANTGATPLSAAVSMR
                     QLEIVERLLEAGAGLEQRLPGEVTVLMLAAALGLPELVVRLLQGGADLHAVDAQGLTP
                     LHCAALYGFTARDRTRLVALLDALLLAGADPQRTAAGGATPLLLLLGARAEPGTPSTE
                     DVVLSGVERLFDEDVRLDARDLRGFGPLHLAGLHGLLRVAQALLREGADPDQRDLLNR
                     TPREIALMRGYVDVAAELALPGNAPQAPVSMARFLRE"
     gene            complement(21487..21756)
                     /locus_tag="PK399_03190"
     CDS             complement(21487..21756)
                     /locus_tag="PK399_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HPW12157.1"
                     /translation="MHAYVYKSLKKADTYAYLARRDDFDCLPEPVRKQLGELRFVLDV
                     ALVEGRKLAQADPAVVRSNLAAQGFHLQFPPTMADPMTEDWGTDA"
     gene            complement(21861..22868)
                     /gene="cydB"
                     /locus_tag="PK399_03195"
     CDS             complement(21861..22868)
                     /gene="cydB"
                     /locus_tag="PK399_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPW12158.1"
                     /translation="MEVWLPVVWFGVIGFGVLMYVLLDGFVLGLGILAPFAQDEHQLD
                     HMMNTAAPIWDGNETWLVLGGAGLLAAFPKAYALILSALYLPVLMMLIALVFRGVAFE
                     FRFKAERSKWLWGWAFALGSLCAAFWQGVILGAIVEGMPLQEGKYIGGMFGFFSPFSM
                     LTGAAVVFGYALLGACWLILKTEGELQKTARTLARPLVLVVVAFIGLVSAWLPFLSSK
                     IMARWFDTGNFWWLSPVPLLVLLVAFALWRAAMAQGRDGKPFILTLCLFALAFAGLVL
                     GIWPYIVPPSLTIWQAASPPSSQGFVLVGLAVLLPVILGYTWWSYRVFAGKVESDSGY
                     H"
     gene            complement(22868..24271)
                     /locus_tag="PK399_03200"
     CDS             complement(22868..24271)
                     /locus_tag="PK399_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPW12159.1"
                     /translation="MDALLLSRIQFGFVISFHILFPAFTIGLASWLAFIEWRWLRTKD
                     DLWRDLYFFWLKIFAVSFGMGVVSGIVMSFQFGTNWAVLSEKAGNILGPLLSYEVLTA
                     FFLEASFLGVMLFGWKKVSDRLHFFATLMVAIGTMISTFWIISANSWMQTPQGFSIDA
                     QGVFQPESWWAIVFNPSFPYRLAHMVLAAFITTCFVIGGVSAWYLRRGVHVEAGKRML
                     KHAVAFAAIAVPLQIFVGDLHGLNVREHQPVKLAAMEAHWRSEPPGHGVPLVLFAVPN
                     AREERNDYEVAIPKLGSLILTHTLDGDIQPLTAVPREERPPVAPVFYAFRVMVGLGMA
                     MLLLTLASLWAWKRKTLFESRWLLDGWRLLAPSGFIALVAGWYVVEIGRQPYVVYGLL
                     RTSEAVSPNIVAAAVLSSLVVYAAAYAFVFGAGIWYLQKLLRKGPVKQPPKDTEGGEK
                     TPARPLSLPDEKIEGGA"
     gene            24439..26808
                     /locus_tag="PK399_03205"
     CDS             24439..26808
                     /locus_tag="PK399_03205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPW12160.1"
                     /translation="MRLAPLAFALLATLVAAPVVHASEPITLTQAMADPDWIGPPVDS
                     GWWRWDGKAAQYLLKRNGATIRDTWQVGIDGGAPARLEGSARADLDTADVVLDSAGMR
                     SAFVRNGDVFVRDLRSGALTQLTRGGDDAGRLQWSRDGALTWRTGSAWQRWDGRVITQ
                     AALPKAEDAPTNPPKPDDLRDRQLRLIATLKDDRARRDAARAQAEAWRSSDPTRAPAA
                     IYLGKDVDIVDSALSPDGAWLLVATTVKGADVGTLGKMPKYVTESGYEEFEDVRTRVG
                     RGDPLPQTLWLAEVATGKVRELKFDALPGIATDPLAALRKAAGKEALKGNRAVQVATS
                     GDNGSAPSLLWSNDGRNAAVMVRAIDNKDRWIATVDLAGATLQPRHRLTDPAWINWGF
                     NEFGWLADGRTLWYLSEESGFSHLYTLDGTNKTQLTDGKWETSAVVPTRDGSSFFFLC
                     NRAQPGDYEVCTVPARGGTVREVTSLDGVESFVPSPDGGKLLVRHSSAYMPPQLSVVD
                     AAGGEARQLTDTRTADFRSRTWIQPQIVQVPSKHGAGTIWGKYYGPATMEPGRKYPVV
                     MFVHGAGYLQNVSARYPNYFREQMFHNLLVDEGYIVLDLDYRASEGYGRDWRTAIYRW
                     MGKPELEDYLDGLDWLVANKQADRDRAGIYGGSYGGFMAFVALFKQPGVFKAGAALRP
                     VSDWSQYNHEYTSNILNTPELDPEAYQRSSPIEYAEGLQDHLLIAHGMIDDNVFFKDS
                     VMLAQRLIELRKDKWELAPYPLERHGFTHPDAWYDEYRRIHELFEGTLK"
BASE COUNT         4295 a         9467 c         9188 g         3940 t
ORIGIN      
        1 acaacgagga cgcgatggcc accgacaaac tcgccgacgg catccgcaag ttcgcagtcg
       61 accagcgcaa gctggaatca ctgctggccg cgaagctggg ctgaggcatc gacgcatgcg
      121 cgaacgccgt ccccacgtcg tcatcgtcgg tggcggcttc gccggtctct gggccacgcg
      181 cgcgcttgga tcggcgaagg tgcgcatcac cctggtcgat cgcggcaacc accacctgtt
      241 ccagccgctg ctgtaccagg tcgccaccgc cggattgtcg gcacccgaca tcgccgcgcc
      301 gctgcgccat atcctgcgca ggcagcgcaa tgccgaagta cggatggcga ccgtcaccgg
      361 catcgatgcg gacgcgaaat gcatcgcgct ggacgatggc acgtcgctcg ggttcgacag
      421 cctgttgctg gccagcggtg ccacgcatgc gtacttcggc aacgaccatt gggccggaca
      481 cgcgcccggc ctgaagacgc tggacgatgc gctggacctg cgccgcaagc tgctgctcgc
      541 cttcgagcgc gccgaagcca ccagcgatcc cgcggagcgt gacgcctggc tgcatttcgc
      601 catcgtcggc ggcggaccca cgggcgtcga actcgccggc acgctggccg agatcgcaag
      661 gcacacgctc aaggatgaat tccgcaacat cgatcccgcg caggcgcggg tgcgcctggt
      721 cgaagccgga ccgcgcgtgc tcgcggcgtt ccccgaagac ctctcggcga aggcgaagcg
      781 ccagctcgaa aagctcggcg tcgaagtgtc gacgggcacg ccggtcaagg acatcaacgc
      841 ggacggttac acgctgggcg acaccgtcgt gcccgcgaag accgtggtct gggcggcagg
      901 cgtggcggcg tcaccgctgg gcgcgatgct cggcgcgccg ctggatcgcg ccggccgcgt
      961 gcaggtgcag ccggacctca gcgtgcccgg tcatccggac atcttcgtcg ccggcgacct
     1021 cgccgctctg agccaggcga atggcaagcc ggtgcccggc gtcgcgcccg ccgccaagca
     1081 gatgggcaac ctcgtcgcgc gaaatatccg cgcgcggctg tcgggccacc cgaccacctt
     1141 gttcgcctac agggactacg gcaacctggc caccatcggc cgcatggcgg cggtggtcga
     1201 cctcggcaag ctgaagttct ccggcctgct ggcgtggtgg ttctggctgg tcatgcacgt
     1261 gttcttcctg atcggcttcc gcaaccgcct ggtcgtgctg ctcaactggt ggtgggcgta
     1321 ctggagctac cagcgcggcg cgcggatcat cctcggcggc gacgccgatg acgtggatca
     1381 ggcgcgcacg acgcgacagg cgtaacaacc cggcttggcg tagtgcaggt gcaggtaggc
     1441 cacgcgttcg atcgcgaaca gcgcggcgat gcctgattcg acgtcgctcc ccggcacgac
     1501 atcggcccag gccatcatcc cgcgtgcgtc gtagccgcgc agcgacagca ggcgcgagcg
     1561 caacagcgcc ggcacttcgc ccaccgtcgg ccgcgcctgc acggcagcct cgcgcacgta
     1621 gatcggtccg gacgcgcgat acggcgtgtc cacgccatgg tgcaggtacg gcaccagcaa
     1681 caggcgttcg ccctcgaccg cgtcctgcag gctgacccgg cagggatagt gcggtttgtc
     1741 gtcggcgacc acccgccgga tgccgcgtgc gcgcagtgct ttgtcgtcag ccgcgaaatc
     1801 ggcggcgaat ggcgcgacgg gcaaggcaag gatccggaat gtcatgcggg tactcctgcg
     1861 tggggacgcc agcatcgcga tccggcgcgc cgtgcgaact ccgtttcttg cgcgtggcat
     1921 gacaagcgcg gacacggctg ccatcctgtc gccacccccg cccggacgga ccgacgtggc
     1981 cttcgccctg cagctgttcg acctggtcgg catcttcgtg ttcgcgctca gcggcggcgt
     2041 gctggcggtg cgccaccgac tcgacctgtt cggcgtgctg gtgctggcct gcgccaccgc
     2101 ggtcaccggc ggcatcgtcc gcgacgtgct gatcggcgcg gtgccgcccg ccgcgctggc
     2161 cgactggacg ctgctgtcca ccgcgatgct ggcgggcgtt gtcacgtttt gctggcacgg
     2221 catcatcgag cgcctgcgca acccggtgct gatgttcgat gccgccggac tcgccctgtt
     2281 cgcggtcctc ggcaccggca aggcgctggc cttcgggctg tcgccgttcg cggccaccct
     2341 gctcggcatc atcaccggca tcggcggcgg catcgcccgc gacctgctgg tggtacgcac
     2401 gccggtggtg ctgcagcaga ccgaactgta tgcggtcgcg gccctggccg gcggcgggat
     2461 cgtcgcgatc gcgcatgcgc tgggctggcc gcaggccccg gcgatggtgg tcggcgcgct
     2521 gctctgcttc ggcctgcgct tcatggcgat ccggcggggc tggcggttgc cggtggcgcg
     2581 cgacgccgac caggcctgat cgcgcaaatg gaaacggccc ggatcgctcc ggaccgtttt
     2641 ctttgcatcg attgcgctgc gcttacgccg cgaacagcgc cttcatcttc ttcagcgcgt
     2701 tggcctcgac ctggcggatg cgttccgcgg agacaccgta ttcgtccgcc agttcctgca
     2761 acgtcacctt gctgtcggca tccaaccagc gacgcttgat gatgtcgcgg ctgcgtgcat
     2821 ccagccgggc catgccttcg cgcagcagtt ccagctggtt gtcctcgtcg ttggccgact
     2881 cgtacgccat cgcgggatcg atgtcattgc tggcgaccaa gtagttgacc ggcgccggcg
     2941 gcgcgttgtc gtcgtcggca tcgtccgggg catcgaagcc cacgtcgcgg cccgagaggc
     3001 gcgattccat ttccagcact tcgcgctcgg acacgttgag gtccttggcg accgcgcgca
     3061 cttcgtccgc gttgagccag cccaggcggg tcttgctctt gcgcaggttg aagaacagct
     3121 tgcgctgcgc cttggtggtc gcgaccttca cgatgcgcca gttcttgatg atgaactcgt
     3181 gcatctccgc gcggatccag tgcacggcga aactgaccag gcgcacgccg acggtcgggt
     3241 cgaaacgctt caccgccttc atcaggccga tgttgccttc ctggatcagg tcgccgatct
     3301 gcaggccgta accgctgtag ccgcgcgcca cgtggatgac gaaacgcagg tgcgacagga
     3361 tcagttcctg cgccgcggcg atgtcgttgt cttcgcgaag gcgacgggcg agacgctgct
     3421 cgtcctcgac ggagaggacg gggatctggt gcacggcgcc gatataggcc tccagcgaac
     3481 ccatggcgct cgggatcggc aggttgttgg cgacgagggc agtggacatg gtggctccgt
     3541 tcatgtgggc gatattagca gtcaccatct gtgactgcta accgtgccga tagttcacta
     3601 ctgttgcatt catggaacgc tggcgcgccc gcgatcccca ttggggcaca ggccccgtca
     3661 accccacgtg gggatggctt ggccccgcca caagccctgg gatgtcgcgc cggctcaggc
     3721 cgccggtgcg gccatgccgg catgccggag cagggcatcg atgctgggtt cgcggccacg
     3781 gaaggcagtg aagttctcca gcgcagtgcg gctgccgccg cgcgacagga cctcggccag
     3841 gaaccgcgcg ccggtctccg ccagccgtgc gggggcttcc tcgaaggccg cgtaggcgtc
     3901 ggcgctgagg acctcggccc atttgtagct gtagtacccg gccgcgtagc cgccagcgaa
     3961 gacatggctg aactgctggg ggaagcggtg ccaggccggc ggccggttga ccgcgacctc
     4021 gtcgcggatg cgctccagca gcgcggcgac gctgtcatcc gccgggtcga agtcgccatg
     4081 gatggccatg tcgaacaggg cgaattccag ttggcgcacc gtggccatgc cgctctggaa
     4141 attgcgggcc gccagcatcc gctcgaacag gtcgcgcggc agcggctcgc cggtgtccac
     4201 atgcgcggtc atcgcctgca cccgcgccca ttcccaacag aagttctcca tgaactggct
     4261 gggcagctcc accgcatccc attccacgcc gttgatgccg gccaccggca acacgtcgac
     4321 cgcggtcagc aactggtgca ggccatggcc catctcgtgg aacagggtgg tcacttcatc
     4381 gtggctgaag gtcgccggct ggccgtccac gcccttgccg aaattgcaga ccaggtggac
     4441 gaggggcgtc tgcgtgccgg cggcagtact ccggcggttg cggccgtcgt ccatccacgc
     4501 gccgccgcgc ttgccgtcgc gggcgtacag gtcgaggtag aactggccga gcaaggtgcc
     4561 agcggaatct tcgacccgga agaaacgcac gtcgggatgc caggtcggcg cactgtcctg
     4621 gcgcacgcga acgccataca aggactggat gacgtcgaac aggccggcaa gcaccgttgg
     4681 ctcggtgaag tagcgcttca cctcctgccc ggaatagccg tagcgcgcct gcttcagctt
     4741 gtcgctggcg tacgcgagat cccagggctg caggtcgtcg atgccgagtt cgtcgcgggc
     4801 gaaggcttcc agctcggtgc gatcccggcg cgcgaacggc agcgcgcgag cggccaggtc
     4861 gcgcaggaaa cccaggacct cggccggcga ctgcgccatc ttggtcgcca gcgaatagtc
     4921 ggcgtaactg gcgaagccga gcagcgcggc cagctccgtg cgcaaggcga ggatgcggtc
     4981 gatgtggccg ctgttgtcga gcgtcggcgg cccgtgttcc gaggcacgta cgccgctggc
     5041 gcgatacagc gcctcacgca ggccgcggtc gtcggcgtag gtctgcaccg gcaggtaaca
     5101 gggcatcttc agggttagcg tccaaccagg cttgccggct ttttcggcgg tcacgcgggc
     5161 ggtggcgacc acgtcggcag gcaggccgga cagccgcgcc tcgtcgtcga cgtgcagggt
     5221 gaaggcgtcc gtcgcatcga gcacgttctg cgagaacgtc gccgacaacg cggagagttc
     5281 ttcctggatc gcggcaaagc gcgccttgcc ggcatcgtcc agctcggcac cgccgaggcg
     5341 gaaatcgcgc agcgcattgt cgacggcctt gcgtcgcgtg gcgtcgcatt gggcgtagtc
     5401 cggcgatgcc gccagtgccc ggtattgcgc gaacagcgcc aggtcctggc ccagcgcggc
     5461 gccgaaccgg gtcaacttcg gcaggttggc gttgtaggcc tcgcgcagtt cgggcgtgtc
     5521 gaccactgcc tgcaggtgcg cgacctgccc ccatgcccgc cacaggcgtt cggtggcgtc
     5581 ctccagcggc gtcacgaagc tggcccacgt caccggcgac acggtctgcg catgtcgcac
     5641 cgctgcttca gcctcggcca acaggctgtc gatggcaggc gtgacgtgtt cggcacggat
     5701 cgcgtcgaaa cgcggcaggc cggagaagtc gagcagcggg ttgtccatga ggggcctgtg
     5761 gaagcgatgc gatgaaaccg agatgcgtgc ctccacgcct caattcaacc aggacggcat
     5821 cgcgcacgat gcagcgcggt cgggagacgg gcgttcaacg ggacgcggac aacgccagcg
     5881 cggcgcgcga tggcagtgcc caatccaccg gcgcctgtcc gttgcgggtg aggtactcgt
     5941 tggctgctga gaaatgcccg cagcccagga agccgcgatg cgccgacagc ggcgagggat
     6001 gcggcgcctt cagcacgcga tggcggcgcg cgtcgatgac cttgcccttg gcctgcgcgt
     6061 agctgcccca gagcaggaac accaggccgt cgcgctcgcg attcagcacg tcgaccacgt
     6121 ggtcggtgaa gccttcccag cccttgccct ggtgcgagcc gggctggccg gcctcgaccg
     6181 tcagcacgct gttgagcagc agcacgcccc gctgcgccca cggcagcagg cagccatggt
     6241 cgggacgcgc gaagccgaca tcgctcgcaa gttccttgta gatgttctgc agcgagggcg
     6301 gcacgtcgac gccgggcagc acagagaaac acagcccgtg cgcctgcccc ggcccgtggt
     6361 acggatcctg gccgaggatc accaccttca ccgcatcgaa gggggtcgca tcgaacgcag
     6421 cgaagatgct ggcgccggcc ggatagatcg tcgcgccggc cgccttgcgc tggcgcagga
     6481 aggcggacag ttgctgcatg tcctcgcggg cgaaccagtc gccgatgcgg gatttccagc
     6541 tcggttcgat ccggatgtca gccatgcatc gtggcggccg ggctcactgc gcgcccggct
     6601 ccggcaattt cgccaggcgc agctggaaca gcaccttggt caccagcagg cgctcctcga
     6661 tcggcttcag caccaggtca ttggcgccat cgcgcagcaa ggaggcctgg ttatcgcggt
     6721 tgtcgtcgcc ggtcatcatc accatcggca gccggcgctt gccgtaaccg aatgcggtgc
     6781 ggatctgcgc cagcagatcg tggccattga ggatgccttt caggtagacg tcgctgagga
     6841 cgagatccgc gccgacgtcg ccatccgtgc gaccgcggaa acggtgcagg tgctccagcg
     6901 catcctccac gctcaggaca tgcagtacct gcatgttctg tcgttccagc atgcgcttgg
     6961 tcgccaccgc caccacccgg ctgtcctcga catacaggac gcgcgcgccc tcgatcggcg
     7021 ccggctgcac gtagccgcgg atgaacgcgg ccagcgcacc gtggcccagc gatttgtcga
     7081 agtagtcggt cacgtcttcg gtgaacgcgc gcgactccag catctcctgc gcgttgccgg
     7141 agaccacgat caccggcacg taggcctgcc cctgcgcctc gcgcacgctg cgcgcaaggg
     7201 cgaggccatc gccgtcgggc aggaccagcg cggtggtcac caggtcgacc gcgcctttgg
     7261 cgagggtctc gcgcgcttcg gcgatgccgg cgcaaccgat cacctcggtg ccgggcaatt
     7321 cgcgcaacaa ggtgtcgccg atgagcttgc gcaccagctt gctgccatcg accaccatga
     7381 cccgtggggc atcgctgacg acgtgacgaa gatcgttggc catgtcagcg ctccaccggg
     7441 cgggtttgcc gcagatagtg gccggtcacc aggccggcgc ccagccagcc gagcacgccc
     7501 gcagccgcga gcacggcgag ggccggcagc ggcggcaagc cctgcaaggc gaaattgctg
     7561 ccgtaacgcg cggcgaggtc ggccaggggt tcgcgcaggg cgagccaggc cagcgtgagc
     7621 acgccgagcg ccagcgcacc cgcgcccagc ccgtaccacg cgccgagata caggaacggc
     7681 cggcgcacga atccgtcgct ggcgcccagc agttgcagca cgccgatttc ctcgcgccgc
     7741 gactggatgt ccaggcgcac ggtgttgccg acgaccagca aggcacccag gccaagcagc
     7801 gccgccagca ccagcaccgc gcgtccaccg aagcgcagcc atgcatccag gcgctcgcgc
     7861 cacaccgcgt cgtgctgcac gcgctcagcg tcgggcaatg ccttcagcga ctccgccagc
     7921 agcaattcgt cgccacgcgg actgacccgc agcacgctcg gcaacgcggc gcgcgccgcg
     7981 tcttcgccca gcgcatcgat cgccgcggcc aggtcgtcgc gtgcgcgcaa ctgctggagc
     8041 gcctgctctg gggcgatgag gtcgaccttc gccacgtcgc cgcgtgcgcg cagggtgtcg
     8101 gcgatggcct gcgcccgggt ggcgtcgatc tcaggtttga ggaacaccgc gacatcgcgc
     8161 gaggactgca cttcgccacc cagtcgctgc aggttctgca ggaccaccca caggcccagc
     8221 ggcagcgcca gcgcgacggc catcacgcct accgtgagca gggtcgccca cggcttgcgc
     8281 aacatgcgac ccaggctggc gacgatgctg aagccgtggt ggtccagcca cgcaccgaag
     8341 cgactggcct gcacgttcgg cgatgcctgg tcattcatcc gccaggtcct ccggcgagat
     8401 gtcgtccagc aactggccgt ggtcgagcac cagcacgcgc ttcttcatcc gtttgaccag
     8461 gcccaggtcg tggctggcca ccagcacgct ggtgccgcgc tcgggcagcg aggcgaacag
     8521 cgccatgatc tcggcggaca gcgtgggatc gaggttgccg gtcggctcgt cggcgatcag
     8581 cagggtcggt tcggcgacca ccgcacgggc gatgccgacg cgctgctgct cgccggcgga
     8641 cagctgcgag ggcaatgccc ggtcgcgcgc gcccaggccg aggcgttcca ggatcgagcg
     8701 cacgcgcttg ccggtttccg cgcgcttcat gccgcgcagg accagcggca gggcgacgtt
     8761 ctcggcgacg ctgcggtcca tcagcaactg gtggttctgg tagaccacgc cgatgctgcg
     8821 ccgatgccgg gcgatggcac caccgcgcac cttcaacagg ttgcggtcgc cgaacagcac
     8881 cgcgccgcga ctgggccgct cggccagctg gatcagcttg agcagggtgc tcttgcccgc
     8941 gccggaatgg ccggtgacga acagcatctc gcccgcgtcc acggcgaagc tcacctcgga
     9001 cagggccgtg tggcctccgg ggtattgctt gctgacgctg tcgaaacgca ggaccgtcat
     9061 cgcgggggat cgtgagcggg cggacaggga gtatcgcccg agccgccggc cgcggccagc
     9121 atgatggctg gcgtcagccg ccgaccagcg acttgatgcg gcggccgatc cggctgaaca
     9181 gcgacggctt gtcgccaccc gctgccggag cgggcgcagt cttgtcgcct gccggcagcg
     9241 gtgcgttcgg cgtggtgccg gcctcgcctt ccagcgggcg gccgtggcga cggcggcgac
     9301 gcttgcgcgg cttgcgcgcg tcgtcctcga tgccggtgct cggcgctgcc ggctgcgcga
     9361 gtgccgcggt ggccggcacc gcggcatcgg caacgaccgt gccggtcacc gtggcagcgg
     9421 ccgccacgtc gccctcgacg cgtggcttgc gcggcggacg cgggccacgc gccggacgat
     9481 cgccggaacg tggcgcggac gaggaacgct cgccgctgcg agcgccgccg ctgcgcggac
     9541 caccacggcc cccgccacgc ttggcgtcct cggccgcgcg cgcctcgcgc acttccttga
     9601 agatctcgcc gatgctctcg ccgtcttcgg cattcgcctc cacgccttcg cgcggcttgc
     9661 gcggcagcgc caccagcatc tccggggtga cgtgctcgcc cgggatcttc tggtcgatgt
     9721 aggcctcgat gtcgggcagc gacatgccgt agcgctcgca ggcgaagctg atcgcgtcgc
     9781 cctctgctcc caggcgggcc gtccggccga tgcggtgcac gtagtcctcg gcatcgaacg
     9841 gcaggtcgta gttgtagacg tgcgacacgc cgtcgatgtg caggccgcgc gcggccacgt
     9901 cggtggcgac caggatttcc agctggccgg cctggaactt cttcagcaac gattcgcgct
     9961 tcttctgcgg cacgtcgccg ctgagcacgc cgacccggta gccggcccgt tccaggccgc
    10021 gggccacgcg ctcgacgaac gccttggtgt tgacgaagac catcgtgcgc gcgccttcgc
    10081 tgcgcgacag caggccgatc agcagcggca gcttctcctc gtcggccggg tagtacagct
    10141 tctgccggac tttggccgcg gtgatgaact cggtttcgac caccagcttt tccggctcgt
    10201 tcatgtgctc gtaggccagc tccagcacgc gatggctgag ggtggcgctg aacagcaggg
    10261 tctgccgggt ggtgcgctcc ggcatccgcc gcagcaggaa gcggatgtcc ttgatgaagc
    10321 ccaggtcgaa catgcggtcg gcttcgtcca gcacgcacat ctcgcaggcg tgcaggctca
    10381 ccaccttgtg ctgcttgacg tagtcgatca gccggcccgg ggtggcgatg atcacgtcgg
    10441 caccgcgctg cagcagctcg cgctgcttgt cgtagtccac gccgccgtac accagggcga
    10501 acttcaggcc gagctggtcg cccagcttca ccgcgtcctt gtggatctgg atcgccagct
    10561 cgcgggtcgg cgccaggatc agcgcgcgcg ggtcctcggc cttgcgatcc gccagcgccg
    10621 ggcgcttgag caggcgatcg acgaccgcga ccaggaaggc cagggtcttg ccggtaccgg
    10681 tctgggcctg gccggcgacg tcgcggccat tcagcgccag cggcagggtc agggcctgga
    10741 tcggggtgca acgggtgaag ccggcggctt ccagcccggc gatcagcgcc ggatgcaggt
    10801 cgaacgagga aaaggtgacg tcggtcaacg gcttgtcgga catgggcggc ctccggcggg
    10861 atgacgccgc gttccagggg acgccgcgat ccgcgcttgc atgaattggg ggcagccaag
    10921 cagactgccg ggacaggttc cgggtctggt cattcccggc acggcacccg ccttggttcg
    10981 ggcgggatgc aaccccatca ttctatcacc ccccggccgg cgcgcccttg cgcacggccc
    11041 cgcgagccat tggagccacc atgagcgata ccgtcctgca cgccaccgac gccgacttcg
    11101 accaccaggt cctggcctca gaccagccgg tgctggtcga tttctgggcg ccctggtgcg
    11161 gcccgtgcaa gatgatcgcg ccggcgctgg acgacctggc cggcacctac gccggccgcg
    11221 ccaagatcgt gaaggtcgat gtcgaccaga accaggctac cgcgctgaag taccacgtgc
    11281 gcagcatccc gatgctcctc ctgttcaagg gcggccaggt gcaggcgacc cagatcggcg
    11341 cggtgggcaa ggcgcagctg gcgcagatga tcgagaagtc gctgtaaatt cgacggacgt
    11401 agcccgggca agcgcagcgc acccgggaac cgccttgcaa cccggacgcg gccttcggcc
    11461 ttgtccgggc gactgaaccc ccgaacgacc ccaacttgcg cgcgatgccg cgccagtgct
    11521 agttttccgc tgaacccggc gccgctccac cgcggaaaca ccgccctcgg gcccgccgca
    11581 ccccttctga tccactcccc acgaccccgc ccgcaatccg cgggcgctcg caacgcaagc
    11641 gaggattttt cccttgtcag acccgaccaa cgacgccggc gaacccgccg agaagcgcgt
    11701 gcgcaagacc cgcgtggcca agaccgccgc gcccaaggac agcttgacca tcccgcccct
    11761 ggaagccgct tcggccgccc cgccagcggc accggcagca acgccggccc caccaccggc
    11821 cgtcgcgccg ccctcggcgc cctcggcgcc cgcgcccgcc accgcctccc atgcgaccca
    11881 cgaaacgcct gcgccgtcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    12001 gcgcccgcgc ccgccaccgc ctcccatgcg acccacgaaa cgcctgcgcc gtcggcaacg
    12061 gcaacgggat cgacggacgg cagtgatgcc ggcggcaacg cccagcagca tggccagcag
    12121 ggccagcacc agaaccgcca caaccacccg aacaaccaga acggcgaagc tggccagcag
    12181 ggcaatcgcc gcgaccgctt caagaaccgc cgcgaccgca atcgcgaccg gccgcgggac
    12241 cagcagggct tcccggacga caacggcggc ggcaacggcg agggcttcgt gccgcgccca
    12301 cacccccagg tgccggaagg cttcccgacc tattcgctcg gcgacctcaa gcggatgccg
    12361 gcgcacaagc tcctggaaat cgccgaccag ctccagatca gcgagggcgt ggcccgcgcg
    12421 cgcaagcagg acatcatctt cggcatcctc aaggtgctga cccgccacgg cgaaggcgtg
    12481 gccgccgacg gcgtgctgga aatcctgccg gacggcttcg gcttcctgcg cgcggccgag
    12541 gcgtcctacc tggccggccc ggacgacacc tacatcagcc ccagccagat ccgccgcttc
    12601 aacctgcgca ccggcgacca catctccggg cgcatccgct ggccgaagga cggcgagcgc
    12661 tatttcgccc tgaacatcgt cgacaccatc aacggcgagc cgctggaagc gtcgaagaac
    12721 aaggcgctgt tcgagaacct gaccccgctg ttcccgcgca agaagttcaa gctggaacgc
    12781 ggcgacggat cgagcgaaga catcaccggg cgcatcctcg acctgatggc cccgcagggc
    12841 aagggccagc gcgccctgat cgtcagcccg ccgaaggccg gcaagacgat gatgatgcag
    12901 cagatcgcca gcgcgatcag ctacaaccat cccgacgtgc acctgatcgt gctgctcatc
    12961 gacgagcgcc cggaagaagt caccgagatg cagcgcaccg tgcgcggcga ggtgatttcc
    13021 tcgaccttcg acgagcccgc ggcgcgacac gtgcaggtcg ccgagatggt gatcgagcgc
    13081 gccaagcgcc tggtcgagca caagaaggac gtggtgatcc tgctcgactc gatcacccgc
    13141 ctggcccgcg cctacaacaa cgtgctgccc agctccggca aggtgctgac cggcggcgtg
    13201 gacgccaacg cgctgcatcg gccgaagcgc ttcttcggcg ccgcacgcaa cgtcgaggaa
    13261 ggcggcagcc tgaccatcat cgcgaccgcc ctgatcgaca ccggcagcgc gatggacaag
    13321 gtgatctacg aggagttcaa gggcaccggc aacagcgaag tgcacctgga ccgccgcatc
    13381 accgagaagc gcgtgtaccc ggcgatcggc gtcaatgcct cgggcacccg ccgcgaggac
    13441 tacctgatcg agcccgacct gctgcagaag atctggatcc tgcgcaagct gctgcacccg
    13501 atggacgaga tcgcggcgat ggaattcctg ctcgacaaga tgaagagcac gaagtccaac
    13561 gacgagttct tcggttcgat gaagcgctga agccagcccg catcgatcat ggaaaagccc
    13621 cgctccgcgg ggctttttcg ttgccgcctg cacggacgtc cgggtcaatc cgacacgctg
    13681 tccaggtccg cttccatgta gcgccactcg ccatcggcgc gcgtgagcga cacgcgtcca
    13741 cggcgcagga tgtcgtcgcg atccttgccc tcgacccaca gcacggcgtc atcgccgtcg
    13801 atgcggccgc gcagcaccct ggcttgcacc ggggtcccgt cgcgcaggtc cttgagcgcc
    13861 gccttgacat cgctttcgtc gtcggacttg atccgccagg cgttgtagtc gcccccgacc
    13921 agcctgcgca gcaccgccag gtcgcccttg gccaccgcgg tcgtccacgc ccgataggcc
    13981 ttgccgggct caccgccgtc ggcgggcagg gcggtgcccg gtggcggggt gatcgcgacg
    14041 tcgaacgtca ggtcgaagtc gtaggtttcg ccgaagaagt cgtccggttc cgccagcacc
    14101 cagcgcccgg cgatacggcc cttgggcgcc gggtcaagct tgaacacgcc gtagcccgag
    14161 gaattgaacg aggcactggg gttgttgtgg gagaagtaga ggccgcattc ggcgccctcg
    14221 gcattgatgc agatgcgcac gtagccggcg ctgccgtcgc ccagttgttc ctccgccgcg
    14281 gagctgggct ggaaggcgga cgcgaccttg cgggcatcca tcggcacgct gctcaggtag
    14341 acataggtct gcgcgctggc ggcgtcgccg tcctcgtcga cggtcaccgc gatgacgtgc
    14401 ttcgcctcca ggcgcacctc gcccttgcgg aaatggccgc tgccgcgatc cgcggcagtc
    14461 gcgacgaggg ccgcgcacaa caacgcggcc ccgaaaccga tttgcacgat gctgcgcatg
    14521 gccttgcccc gatctgccgt ggatacaggc tgcaacgcag ggcaggcggc acagcggccg
    14581 ccgccgcggg ggggggggcg ctcagccagc ggggatgggc gtgaccttga cgggggaatt
    14641 ggtggcgatg ggcaacggtc cgcccggccc cgaggcggcg acgcattcct tcgattccag
    14701 cgcgcgcgcc tccagttgct cccggtactc gtcgttgctg cgcggcccgc tcttcgcgga
    14761 cgacagcatg aaactcaccg ggaacctcat ctcggtggac accggcctcc cgccgacctg
    14821 ctccggctgg tagcgcatgc cttcgatcca gctggtcaag gtggcgcgga agggcttcac
    14881 gaagcggccg agggtgccct ccatcggttc gatcgaggca agccgggtgg tgccgtcggc
    14941 ctggatcgaa taggtcaccc ggaacgtgcc ttccgcgccg cgccgccgca gttcgaacgg
    15001 atagtccggc ggagggatga accacggacc ctgggagacg agggccggcc cgttgccctt
    15061 gaagtccagg cccaggcggt agccctccgc ggccgggatc gcacaggcgc cgaccttgac
    15121 gaaggtgacg gccgacgcag gcacgccgtc caccttggcg ggctcgtact gccaggaggc
    15181 gacgcgcttc tcgatgaagg cgcggatggc caccggcagg tcggccggcg cttcgacctt
    15241 gaccggcttg cccgcggcgt ccacctcgac gcgcacggac gcattgaagg ccaccacgtc
    15301 cggcttggcc gggtcgtcgg ccgcgatcgc cggcgaggcc gccatgcagg cgatcgcgac
    15361 acacagcatc ggggtcttca tgctcagccc agcggggtga cgagggtcgc gccctggcgc
    15421 aacggataca cgccgttgtc gggcagcgcg tcggggctgc ccgcggtctt cagtttttcc
    15481 tgcgcccagg gcatgtccgt gttgcgcggg ccggggatgt aggtatccca accgccctcg
    15541 ttgcggcgcc tgtcgctggg cctgcccagg acgaactcga cggggacgcg caccagccag
    15601 ctgtcgtcgt ccgcgccctc gcccgtggtc ggcacggcga aggaccagcc gcgcgcaacc
    15661 cgcagggccg ccatcgtgaa ctggtcgcgc aggctgtcca tctcgttggc ggtgccggcg
    15721 acgcgcaggt tgacctgctc cgcggcgaca ttggccacct tgccgtcgcg accgacctgc
    15781 accaccaggt agaccgtgcc ctggccaccc atgcgcagtg cgctcttggg gaattgcggg
    15841 ggagacagct tgatgcccgc cgggttgtcg gtcttcggga ccgcatcggt gctgccgaaa
    15901 tagctgctgg cgatggccac gctgacctgg tcgccttcgg cgcgtttggc gaccagccgc
    15961 aggtgcatcg gcaccttggc attcaccggc gcgccatcga cgagcaccgg ctggaagcgc
    16021 caacctgcta ccgccttgcc gacgaaggcg gtgagcgcgt cgcccagcgg cgcttcggga
    16081 tcgagggtgt gggcgatgac gctgccgtcc ttgccgatgg tgatcgtgcc cgacacctgc
    16141 aggctggatt cggcctgctt gcgcaccgcg gcggcgccac gcgaaccgga cagtgccggc
    16201 atcgccagca gcaatccgcc cagcaaggcg atggcggcca tcagacgcat cttcatcgtg
    16261 caacctccct gtatgacggc cggagtatgc cagtgccggc aacagccctg atcgccgata
    16321 ctgaagtccc tgaacggagt catccggcat gcacggcgaa ggcctcgagc tcgcactggt
    16381 attcctgctc gcggcggtgg tcgcggtgcc ggtgttcaag cgcttcgggc tgggcgcggt
    16441 gctcggctac ctggtcgcag gtgcgttgct cgggccgcac gcgctgcggc tggtgcgcaa
    16501 cccggagccg gtgctcgccg ccagcgagat cggcgtggtg atgctgctgt tcgtgatcgg
    16561 gctggaactg tcgctgccgc gcctgaaggt gatgcgcagg ccgatcttcg gcgtcggcgg
    16621 cctgcaggtg ctgctgtcgg ggctggcgct gggcgcgctg gcgatgtcgc aggacctggg
    16681 ctggaaggcg gccctggtgg tcggcctggg cctggcgctg tcgtcgaccg cggtgggcct
    16741 gcaactgctc tccgagcgca aggaactcac tgcggaacac gggcggctgg ccttcgccat
    16801 cctgctgttc caggacctgg ccgcgatccc gctgctggcc gcgatcccgc tgctcggcaa
    16861 ggccggtggc gagggcctgg gctgggagga cgtggcgaag gcggtactgg cgatcgccgc
    16921 gctggtgctg ggtggccgtt atctgttgcg ccacctgttc cggatcatcg cgcgcaccgg
    16981 catgccggag gtgttcaccg gcgcggccct gctggtggtg ctgggcagtg cctgggtgat
    17041 gcaactggcc gggttgagcg ccggcctggg cgcgttcctg gccggcgtgc tgctggcgga
    17101 gtcggaatac cggcatgagc tggaagcgca gatcaagccg ttcgaaggcc tgctgctggg
    17161 cctgttcttc atggcggtgg ggatgaccat cgacctgcag cagatcgcca gcgagccgct
    17221 ggtcatcgcc gcctgggtcg cggcattgct ggcggtgaag ttcgcgctgc tgttcgcgct
    17281 tgggcttcgc ccgggaaagc tcgacctgcg tggcgcgatg ctgctcggcg gcgtgctggc
    17341 gctgggcggc gaattcgcct tcgtggtgtt cgccgaagcc atgaaggcgg gcctgttggt
    17401 cgaacccctg cgcgaccggc tggtcgccgt ggtcgggctg tcgatggcgg ccacgccact
    17461 gttgctgttg ctgatctcgc gcgtcgcacc cgcggtggaa aagcgccggc ggcgcgcgta
    17521 cgacgacatc ccggacgaac atccgcaggt gctgatcgcc ggcttcggcc gcttcgggca
    17581 gatcgtggcg cgcctgctgt gggcgcagaa gatcccgttc atcgcgatcg atcccgacga
    17641 cgaacaagtc gatttcttcc gccgcttcgg caaccaggtc tactacggcg acccgaccca
    17701 ccccgaactg ttgcgctccg ctggcgcggc gcgggtcagg gtgttcgtgg tcgccatcga
    17761 tgacgtggac aaggcgacca cgctggtgcg cctgttgcgc aagcactacc cggatgcacg
    17821 ggtgctcgcg cgcgcgcgcg accgccggca tgcgtggcag ttgctggacc tgggggtgaa
    17881 gccgttccgc gaactgttcg gcagcagcct ggagatgggc cgagaggtgc tggtcgcact
    17941 gggcatggag gcgcgccagg ccacggagta cgcaagccgc ttccgcgaat tcgacgaacg
    18001 cgtgctggat gcacagcggc tgatgcagga cgacgaggac gccctgctgc aaagtgcgag
    18061 gaatgcacgc cgcgaactcg aggagttgtt cgaggcggac gccggcgaag gcacgctggg
    18121 acgcatcgtc gaacgcaagg actgaatccg gtcagcggcc gacgccgccg ttattcccgc
    18181 aggaagcgcg ccatcgagac aggcgcttgc ggcgcgttcc cgggcagtgc cagctcggcg
    18241 gcgacgtcca cgtagccgcg catcagggcg atctcccgcg gggtgcggtt gagcaggtcg
    18301 cgctggtccg gatcggcacc ctcgcgcagc agcgcctgcg ccacccgcaa cagcccgtgc
    18361 aggccggcca ggtgcagcgg gccgaacccg cgcaggtcgc gcgcatccag gcgcacgtcc
    18421 tcgtcgaaca gccgctccac gccggacagc accacgtcct cggtgctggg cgtgccgggc
    18481 tcggcgcgcg cacccagcaa caacagcagt ggcgtggcgc cgccggcggc ggtgcgctgc
    18541 ggatcggcgc cggccagcag caacgcatcc agcaacgcga ccaggcgggt gcggtcgcgt
    18601 gcggtgaagc cgtagagcgc ggcgcaatgc agcggcgtca ggccctgcgc atcgaccgca
    18661 tgcaggtcgg cgcccccctg cagcaggcga acgaccagtt ccggcaggcc cagtgcggcg
    18721 gccagcatca gcacggtgac ttcgcccggc agccgctgct ccaggccggc cccggcctcc
    18781 agcaggcgct cgacgatctc gagctggcgc atgctcaccg cggccgacag cggggtagcc
    18841 ccggtattgg cggcaagggt gggatcggca ccccgctcca gcagcagttc gaccagcgcg
    18901 cgatggccgc caccggccgc gcgcagcaag gcgctgcagc cctggctgtc ggtcgcgtcc
    18961 accggcagcc cgaggtcgag gaggcggcgg accgcgtcga ggtcgcccac gatcgccgcg
    19021 gctggcacgt ccagtggttg cagcggtcgc cgcggcagct tccagccgcg ccagtccagc
    19081 cagccggcca ggtcgcgccg tcccgccgcc agggcgacgc ccagcggggt ctggccgtcc
    19141 gccgcgcgcc ggtcgggcgc ggcgccagcg gcgagcagct gcttgagttc gtcgagccgg
    19201 cccagcgcgg cggccaggtg cagcgcgctc atcccgtggc tgtcgcgggc gtccaggtcc
    19261 acgccgtggg ccaccagccg ctgcagcagg cgcggccagt gcaggcgcac cgccagcgcc
    19321 accggcggat cgccggccgg cgaggtcgca taggggtcgg cgccacgctc cagcagctcc
    19381 agtgcgaaac cctcgccccg ggcgtcggtg aagccttcag tcaggcaggc cgccaggtag
    19441 acggccaggc cgccggcacc ggcgggcgac gccccccggg cgaacagcag gcgggcgttg
    19501 tcgatgccgg ccgtgccgcg cgccagcgcc gcggccaccg gcatcggcgc cgatcggacc
    19561 cgcgcttcgg ggttggcccc acgggccagc agccaggcga cgcgcgcggg atcggcggcg
    19621 acatcggcat cgcagagcag gccatcgagg tcgccgcggg ccagcagcgg cagcagcgtc
    19681 gccagctggt tttccccggc ctcgtccagc agccgctcgc gcagcagcaa cggcggcggt
    19741 cgatccggtg caccgtcgtc ctcgtccatc gacaccggca gcgcgcgttg cggatccagc
    19801 gcggcgacca gcgtccagcg gcccgcggcc gatgccagat cgatcgcgcc ctggccttgc
    19861 gcatccggcg cgcgcggatc gacgccgagt tcgagcaggc gcctcaccat ggtcgccgac
    19921 ggcgcttcgg cgatgcaggc caggtgcagg gcattcgcac cctgtccatc gctggcgtgc
    19981 acatcggcct tggccgcgac cagcacctcc aatgccggga gcgcaccggc gcgggtggca
    20041 tcgagcagcg gggtgcgccc ttcgccatca cgcacgttgg cgtccgcacc ggcatcgagc
    20101 agggcttcgc agatgccggc atggccggcg aacgccgctt catgcagggc agtacggccg
    20161 cgggcgtcgc gggcatccac gcgcgccttg tggcgcagca gcagttgcac gccggcggcg
    20221 tcgtcttctt cggtgccggc ggcggcgagc agcaccggtt gcccgccgac cggttcggtc
    20281 ttcgccccgc gctccagcag gaagcgcgcg agtcgccagt tgccggcact gcaggccgtg
    20341 cccagcggcg acacggcctc gatgttcaac gcgtccattt ccgcaccggc gtcacgcagc
    20401 agcgcggcca ccccgggatc ggagctgcgc gcggcatggt gcagcggggt gttgccgtcg
    20461 gcgtcggtgg cgcgcgggtc ggcgccattg gcgagcaggg tcatcaccgc ttccggccgg
    20521 ccgtgccagc tgtcgcgggt cgccgccagc agcgggttca tcccggccag catgaggttc
    20581 acgtcggcgc cgcgctcgat caaggcgcgc agcaggcgca ggtcgggcag gacggcggcc
    20641 agcaccggca agccgcgacg atcgcgcgca cccacctcgg gcggcaggtt cgggtcggcg
    20701 ccggcctcca gcaattgcag ggctttctcg acccggccat tgcgcgccgc cgcgtacagc
    20761 tcgcggccca gggccacggt gtcgaccacc ggcaccgtcg ccagcggcag cgcgagttcc
    20821 ggtgcgtcgt cgaggtcggc gacgaggtcg tccgcctcgg cttcgacggc gtccatctcc
    20881 accaccggca acccatgcgc caacgacagg gtgggcgcgg cctgcagcag caggtgggcg
    20941 atcggcagca ggccggcgta gaccgcggtg gcgacgatgc gcacctggcc ggccagcagg
    21001 ccgggccagg ccagcagcag gccaccgacc agcagcagga acaccggcaa ggcgacttgc
    21061 accagcgcgc gccaggcctg gcgatcctgc aggtgctggc gggcgaagcg caggcccagg
    21121 cggccgcctt cgcgttcgct gccgtgccag agcggccagt gccaccacag caggaccagc
    21181 agcaggccgg ccgccgcact cagtgcgagc acaggcaaca aggccggcgc ggccaggaca
    21241 tgctgcagcg gccaggcgac cagcacgccg gccagtacga agccgccgcc ccacagcagg
    21301 gccaggctgc cggcatcggc cgccagccgc gcacgcttgc gggtcccgcg caggaaggcg
    21361 atgcccagcg ccatcggcac ctgcgccagc catgccgccg cgccggcgct gccgacgtcg
    21421 aaccagcacg cgccggccag cagcaggccg gccagcaaca gggccacgcc aatcccggga
    21481 tgccgctcag gcatcggtgc cccaatcctc ggtcatcggg tccgccatgg tcggcgggaa
    21541 ctgcaggtgg aagccttgcg cggcaaggtt gctgcgcacc accgcggggt cggcctgggc
    21601 cagcttgcgg ccctcgacca gggcgacatc cagcacgaag cgcaactcgc ccagctgctt
    21661 gcgcactggc tcgggcaggc agtcgaagtc atcgcggcgc gccaggtagg cgtaggtgtc
    21721 ggcctttttc aggctcttgt agacgtaggc gtgcatcgtg cgatccgggg gaaccgccgt
    21781 gtcgggaaac aggcccgatt gtgcgggaaa gcgcgcggcg gcgcgacccg ccggggctgc
    21841 gcggcgacgc cgcaagcgag tcagtggtaa cccgagtccg attccacctt gccggcgaac
    21901 acccggtacg accaccacgt gtagcccagg atcaccggca gcaggaccgc cagcccgacc
    21961 agcacgaagc cctgcgagga cggcggcgac gcggcctgcc agatcgtcag cgacggcggc
    22021 acgatgtacg gccagatccc cagcaccagc cccgcgaagg caagcgcgaa caggcaaagg
    22081 gtgaggatga acggcttgcc atcgcggccc tgcgccatcg ccgctcgcca cagcgcgaac
    22141 gccaccagca gcaccagcag cggcaccggc gacagccacc agaagttccc ggtgtcgaac
    22201 caacgcgcca tgatcttcga gctcaggaac ggcagccatg cgctgaccag gccgatgaac
    22261 gcgaccacca ccaacaccag cggccgcgcc agcgtgcgcg cggtcttctg cagttcgcct
    22321 tcggtcttca ggatcagcca gcacgccccg agcaacgcat aaccgaacac caccgcagcg
    22381 ccggtcagca tcgagaacgg actgaagaac ccgaacatcc cgccgatgta cttgccctcc
    22441 tgcagcggca tgccctcgac gatcgcgccc aggatcacgc cttgccagaa cgccgcgcac
    22501 agcgagccca gcgcgaatgc ccaaccccac agccacttcg atcgctccgc cttgaagcgg
    22561 aactcgaagg cgacgccgcg gaacaccagc gcgatcagca tcatcagcac cggcagatac
    22621 agggccgaca ggatcaaggc atacgccttg gggaacgccg cgagcaggcc ggcaccaccg
    22681 aggaccagcc aggtctcgtt gccgtcccag atcggtgccg cggtgttcat catgtggtcg
    22741 agctggtgct cgtcctgcgc gaacggggca aggatgccga ggcccagcac gaaaccgtcc
    22801 agcagtacgt acatcagcac gccgaagccg atcaccccga accagacgac gggaagccag
    22861 acctccatca cgcgccccct tcgatttttt catcgggcaa cgacaacggc cgtgccggcg
    22921 tcttctcgcc gccttcggtg tccttcggcg gttgcttcac cggccccttg cgcagcagct
    22981 tctgcaggta ccagatgccg gcgccgaaca cgaacgcgta ggccgcggcg tacacgacca
    23041 gcgaggacag caccgccgcc gcgacgatgt tcgggctcac cgcttcgctc gtgcgcagca
    23101 ggccgtacac gacgtacggc tggcgaccga tttcgaccac gtaccagccc gccaccaatg
    23161 cgatgaagcc cgatggcgcc agcaatcgcc agccatccag cagccagcga ctctcgaaca
    23221 gggtcttgcg cttccacgcc cacagcgagg caagggtgag cagcagcatc gccatgccca
    23281 ggccgaccat cacccggaac gcatagaaca ccggcgccac cggcggccgc tcctcgcgcg
    23341 ggaccgcggt cagcggctgg atgtcgccat ccagggtgtg ggtgaggatg aggctgccga
    23401 gtttcgggat cgcgacctcg tagtcgttgc gttcctcacg cgcgttcggc accgcgaaca
    23461 gcaccagcgg cacaccgtgt cccggcggct cgctgcgcca gtgcgcttcc atcgccgcca
    23521 gcttcaccgg ctggtgctcg cgcacgttca ggccgtgcag gtcgccgacg aagatctgca
    23581 gcggcaccgc gatcgcggcg aaggccaccg catgcttgag catccgcttg ccggcctcga
    23641 catgcacgcc gcgccgcagg taccacgcac tcaccccacc gatcacgaaa caggtggtga
    23701 tgaacgccgc cagcaccatg tgcgccagcc ggtacgggaa cgacgggttg aacacgatcg
    23761 cccaccagct ttccggctgg aacacccctt gcgcgtcgat gctgaagccc tgcggcgtct
    23821 gcatccagct gttggcggag atgatccaga acgtcgagat catggtgccg atggcgacca
    23881 tcagcgtcgc gaagaaatgc agcctgtcgg agaccttctt ccagccgaac agcatcaccc
    23941 cgaggaagct ggcctcgagg aagaacgcgg tgagcacttc gtaactgagc agcgggccga
    24001 ggatgttgcc ggccttctcg ctgagcaccg cccagttggt gccgaactgg aagctcatga
    24061 cgatgccgct gaccacgccc atgccgaacg acacggcgaa gatcttcagc cagaagaaat
    24121 acaggtcgcg ccagaggtcg tccttggtgc gcagccagcg ccattcgatg aaggccaacc
    24181 aactcgcgag gccaatggtg aaggccggga acaggatgtg gaacgaaatc acgaagccga
    24241 actggatccg agacagcaaa agcgcgtcca cgcaatgcac tccacggcgc atggggggga
    24301 cgccgcccgc atattgtgcg cccggcggat gagagccgcc atgcaaacaa gctatcgcac
    24361 cgattgagcg ggacttccgg cggacgcctg cgcaggcacg gctgctaccc tttgccgacc
    24421 tgcttcccgg atcgccccat gcgccttgcc ccgctcgcct tcgccctgct cgcgaccctg
    24481 gtcgccgcgc cggtggtcca tgcctccgaa ccgatcacgt tgacccaggc gatggcggat
    24541 ccggactgga tcgggccgcc ggtggacagc ggctggtggc gttgggatgg caaggccgcg
    24601 cagtacctgc tcaagcgcaa cggcgcgacc atccgcgaca cctggcaggt cggcatcgac
    24661 ggcggtgcac cggcgcgact ggaaggcagc gcacgcgccg acctcgatac cgccgatgtt
    24721 gtcctcgatt cggccggcat gcgcagcgca ttcgtgcgca acggcgacgt gttcgtgcgc
    24781 gacctgcgca gcggcgcgct gacccagctc acgcgcggcg gtgacgatgc cggccgcctg
    24841 caatggagtc gcgacggcgc actgacttgg cgcactggtt cggcgtggca acgctgggat
    24901 ggtcgtgtca tcacccaggc ggcactgccg aaggccgagg atgcgccaac caacccgccc
    24961 aagcctgatg acctgcgcga ccgccagctg cgcctgatcg caacgctgaa ggacgaccgt
    25021 gcgcgccgcg acgccgcgcg cgcgcaggcc gaggcctggc gcagcagcga tccgacccgc
    25081 gcgcccgcag cgatctacct cggcaaggac gtcgacatcg tcgacagcgc gctgtcgccg
    25141 gatggtgcgt ggctgctggt cgcgacgacg gtgaagggcg ccgacgtggg cacgctcggc
    25201 aagatgccga agtacgtcac cgaatccggc tacgaggaat tcgaggacgt gcgcacgcgc
    25261 gtgggccgcg gcgatccgct gccacagaca ctctggctgg ccgaggtcgc caccggcaag
    25321 gtccgtgaac tcaagttcga cgcactgccc ggcatcgcca ccgatccatt ggcggcactg
    25381 cgcaaggccg ccggcaagga ggcgctgaag ggcaaccgcg cggtgcaggt cgcgaccagc
    25441 ggcgacaacg gcagcgcgcc atcgttgctg tggagcaacg acggccgcaa cgcggccgtg
    25501 atggtgcgtg cgatcgacaa caaggatcgc tggatcgcca cggtcgatct tgcaggcgcg
    25561 acgctgcagc cgcgccatcg gctcaccgac ccggcgtgga tcaactgggg cttcaacgaa
    25621 ttcggctggc tcgccgatgg ccgcacgctc tggtacctga gcgaggaaag cgggttctcg
    25681 cacctgtata cgctcgacgg cacgaacaag acccagctta ccgatggcaa gtgggaaact
    25741 tccgccgtcg tcccgacgcg cgatggatcc agcttcttct tcctgtgcaa ccgcgcgcag
    25801 ccgggcgact acgaagtctg caccgtgccc gcgcgcggcg gcacggtgcg cgaagtcacc
    25861 tcgctggatg gcgtggaaag cttcgtgccc tcgccggatg gcgggaagct gctggtgcgg
    25921 cattcgtccg cgtacatgcc gccgcagctg tcggtcgtcg atgcggctgg cggcgaagcc
    25981 agacaactca ccgacacccg caccgccgac ttcaggtccc gcacctggat ccagccgcag
    26041 atcgtgcagg tgccgtcgaa acatggcgcc ggcaccatct ggggcaagta ttacggcccg
    26101 gcgacgatgg agccgggcag gaagtatccc gtggtgatgt tcgtccacgg cgccggctac
    26161 ctgcagaacg tcagcgcgcg ctatccgaac tatttccgcg agcagatgtt ccacaacctg
    26221 ctggtcgacg agggctacat cgtcctcgac ctcgactatc gcgcctccga aggctacggc
    26281 cgcgactggc gcaccgcgat ctaccgctgg atgggcaagc cggagctgga ggattacctg
    26341 gacggcttgg actggctggt cgccaacaag caggccgacc gcgaccgcgc cggcatctac
    26401 ggcggcagct acggcggctt catggccttc gtcgcgctgt tcaagcagcc cggcgtgttc
    26461 aaggccggcg ccgcgctgcg cccggtcagc gactggtcgc agtacaacca cgaatacacc
    26521 agcaacatcc tcaacacccc ggaactcgat cccgaggcct accagcgctc cagcccgatc
    26581 gaatacgccg aaggcctgca ggaccacctg ctgatcgccc acggcatgat cgacgacaac
    26641 gtgttcttca aggactcggt gatgctggcg cagcgcctga tcgaactgcg caaggacaag
    26701 tgggaactcg cgccttatcc gctggagcgc cacggcttca cccacccgga tgcgtggtac
    26761 gacgagtatc gccgcatcca cgagttgttc gagggcaccc tgaagtaagc cctgcccatg
    26821 ctgcgcatca ccgaactcaa gttgccgctg gaccatgacg acgccgcgct ggccgcggcg
    26881 atcgtcgcgc gcctgggcat ccgcgccgat gaactggccg gctacaccgt cgccaagcgc
    26941 agctacgacg cgcgcaagcg cggagcgatc gtgctgatct actcggtgga
//
LOCUS       DAOOCX010000019        26923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1458_length_26923_cov_7.602687, whole genome shotgun sequence.
ACCESSION   DAOOCX010000019 DAOOCX010000000
VERSION     DAOOCX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 26923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26923
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1458_length_26923_cov_7.602687"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..221
                     /gene="rpmF"
                     /locus_tag="PK399_03210"
     CDS             27..221
                     /gene="rpmF"
                     /locus_tag="PK399_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPW12161.1"
                     /translation="MAVQKSRVSPSRRGMRRAHDALSAKQLATDPTSGETHLRHHVTA
                     DGYYRGKQVIAPKTKVADEE"
     gene            404..1393
                     /locus_tag="PK399_03215"
     CDS             404..1393
                     /locus_tag="PK399_03215"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPW12162.1"
                     /translation="MSDSQGRVYSRIAGTGSYLPDKVLTNADLTEFVETSDEWIVART
                     GIRERHVAAEGETTSDLGYQAALRAMEAAGVTAAEIDLIILGTTTPDLIFPSSACLLQ
                     HKLGANGCPAFDVNAACSGFIYALTIADKFIQSGAAKTALVVGAETLTRMLDWNDRGT
                     CVLFGDGAGAVVLKADAETGILSTHMHADGGKKELLWNPVGVSVGFKPDEKNAGVRVL
                     MTGNEVFKHAVKALDSVVEEALDANGMDRHDIDWLVPHQANLRIIEATAKRLDMPMDR
                     VIVTVDRHGNTSSGSVPLALDEAVRSGKIQRGQVVLLEAFGGGFTWGSALLRY"
     gene            1761..>2554
                     /gene="fabD"
                     /locus_tag="PK399_03220"
     CDS             1761..>2554
                     /gene="fabD"
                     /locus_tag="PK399_03220"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPW12163.1"
                     /translation="MLAELSELHPGMKATFEEASAGAGVDLWALSQGGPEELLNRTEY
                     TQPALLAAGVAVWRAWQASGGPAPDVLAGHSLGEYTALVAAGALSLHDGAHLVRIRGQ
                     LMQDAAPAGTGAMAAILGADDALVAEVCEAASGTQVVVPANFNSPGQIVIGGHAAAVD
                     AALAMLAERGVRKAIKLAVSVPSHTPLMRDAANRLAETMAGLQWRMPSLPVVQNVDAT
                     VHDSVDAIRDALVRQLYLPVQWTGCVQALAARGVGRIGECGPGKVLA"
     assembly_gap    2555..2654
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2655..2803
                     /locus_tag="PK399_03225"
     CDS             <2655..2803
                     /locus_tag="PK399_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HPW12164.1"
                     /translation="AARGVGRIGECGPGKVLAGLVKRIDKSLDARALGTPAEFAGAIE
                     EWNA"
     gene            2800..3546
                     /gene="fabG"
                     /locus_tag="PK399_03230"
     CDS             2800..3546
                     /gene="fabG"
                     /locus_tag="PK399_03230"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPW12165.1"
                     /translation="MSELVLKGEVALVTGASRGIGAAIADELAALGATVIGTATSDAG
                     AQAIGERLAAAGGHGRRLNVNETGSVEALIDAVSTEFGGVSILVNNAGITRDNLLMRM
                     KDEDWQAILDTNLTSVYRASKAVMRGMMKARKGRIINIASVIGVTGNAGQSNYAAAKA
                     GIIAFSKSLAKEIGSRGITVNVVAPGFIATDMTADLPEAAKEAMLGQIALGRLGEPAD
                     IARAVAFLAGPGATYITGETLHVNGGMYMP"
     gene            3660..3899
                     /gene="acpP"
                     /locus_tag="PK399_03235"
     CDS             3660..3899
                     /gene="acpP"
                     /locus_tag="PK399_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPW12166.1"
                     /translation="MSNIEERVKKIVIEQLGVKEEDVSNSASFVEDLGADSLDTVELV
                     MALEEEFECEIPDEEAEKISTVQAAIDYIKAHVKA"
     gene            4007..5245
                     /gene="fabF"
                     /locus_tag="PK399_03240"
     CDS             4007..5245
                     /gene="fabF"
                     /locus_tag="PK399_03240"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPW12167.1"
                     /translation="MSKRRVVVTGMGIVSPLGNDLASNWDGIVNGRSGIGPVAGFDAS
                     TYPTRIAGEIRDFDVTRWVNPKDAKKMDHFIHYGVAASLMALEDSGLQVTEANAERVG
                     ALIGSGIGGIWGIEETSVKLHEGGVRKISPFYIPSTIINMLPGQVSLLTGIKGPNFSA
                     VSACATANHSIGMAMRMIQHGDADVMIAGGAERGSSPTSMGGFCAMKAMSTRNDDPAA
                     ASRPWDAGRDGFVLGDGAGILVLEEYEHAKARGARIYCELAGFGASSDAFHMTAPSEN
                     GDGPARCMAMAFRDAGINADEVGYLNAHGTSTPLGDLAETLAIKRALGDHAYKTMVSS
                     TKSMTGHLLGAAGGVEAIYTIKALETGIIPPTINLETPSEGCDLDYVPNVAREARVDV
                     VVSNGFGFGGTNGTLVFRRI"
     gene            5301..6650
                     /locus_tag="PK399_03245"
     CDS             5301..6650
                     /locus_tag="PK399_03245"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017160233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HPW12168.1"
                     /translation="MITRTLPADVDLLDLHRLDPARYPVLLESSATGAHGRWDMLLAH
                     DGESFVLHRDGMVRDQDGGWIDGRFLDVLDARWQAQRLPRAEVDTLPFRGGWALLLGY
                     ELAQQVEPVLRLPPAAGGLPIACALRCPAAVLRDRVTGACFAVAEADASAWLGRIGAD
                     LARAGSAGSLPDWQAPAGIAEEPSQRYVDGVQRVLGYLAAGDVFQVNLSRGWTARFDS
                     PLAPAALFQRLRGNNPAPFAGIFRTDAGSVVSASPERLVSVRGDVVETRPIAGTRPRF
                     AGDDDGERIRELVGHPKERAEHVMLIDLERNDLGRVCTPGSVVVDELMTVESYAHVHH
                     IVSNVRGTLLPDASPGQVIAAVFPGGTITGCPKVRCMQIVAELEGAGRGAYTGAMGWL
                     NRDGDMDLNILIRSGEVEGQELRFRTGAGIVIDSDPQRELDETRAKARGMLRALGVE"
     gene            6647..7465
                     /gene="pabC"
                     /locus_tag="PK399_03250"
     CDS             6647..7465
                     /gene="pabC"
                     /locus_tag="PK399_03250"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR03461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HPW12169.1"
                     /translation="MIAVAYIGREPMDAVSIGNRGLNYGDGVFETMRVHRGVLPLWPR
                     HLARLREGAGRLGIAMPDVGVMEARIAELMAGADAGVLKLLLTRGEGGRGYAPPLDAV
                     PTWMLSLLPLPAPQGALRLHACEVRLAMQPALAGIKHCNRLEQVLGRAEAERAGCDEG
                     LMRDMVGNPVCATSANLLVLRDGRWTTPPVDLCGVAGVLRGWLLDQGLVALASLSMDD
                     VESAQALALCNAVRGILPVRALGAREWPTHPAVADLQKRLAMATPMFSESRETA"
     gene            7462..8520
                     /gene="mltG"
                     /locus_tag="PK399_03255"
     CDS             7462..8520
                     /gene="mltG"
                     /locus_tag="PK399_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPW12170.1"
                     /translation="MKPRVAGGAFARFLLVLLLAGLAVAGWQWQRYQGFADAPLAGID
                     AGDSLVVARGDSLDKVLRTLQAQGVDTGDRVQWQVLARQTGAAGKLQVGEYALAPDTS
                     PRALLLAMRDGKVVRRNFTIVEGWNIRDVRAALAKVEGLEHDSAGLDDAALMKALGHP
                     GQHPEGRFLPETYAWVKGDSDLDLLKRAHKAMQEALDEAWASRADDLPLKNADEALVL
                     ASIVEKETGIAEERPAIAGVFVRRLQIGMRLQTDPTVIYGMGAAYAGNIRRSDLTTDT
                     PYNTYTRAGLTPTPIAMPGKAALRAATRPADGDALYFVAVGDGSGRHVFSASLDAHNA
                     AVRDYVQRYRAQRQAAPK"
     gene            8517..9176
                     /gene="tmk"
                     /locus_tag="PK399_03260"
     CDS             8517..9176
                     /gene="tmk"
                     /locus_tag="PK399_03260"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPW12171.1"
                     /translation="MTAALQRQPRLLSIEGGEGAGKSTVLSALRDALLADGQEVVSTR
                     EPGGTPLAERIRELLLGGTGETVHPQTELLLMFASRAQHVREVVLPALQRGAWVISDR
                     FTDSSYAYQGAGRGLDEGYIAALEQQAVGIEPGLTLLLDLGVAHGRERTRGRDLLGGS
                     VPDRIERERDSFFERVRDGFLARAAQHPDRFRVIDASHDAASVAAAALAALADYLGTL
                     R"
     gene            9179..10180
                     /locus_tag="PK399_03265"
     CDS             9179..10180
                     /locus_tag="PK399_03265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007969013.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the delta' subunit seems to
                     interact with the gamma subunit to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPW12172.1"
                     /translation="MLPSLSPWQQRAYDHATAAIDGGHFGHATLITGPALIGKRVLAE
                     HLAHRVLCRSPQANGEPCGACKGCSLFAARAQYDPLEVRPDGTPSHPWGHSAHPDLLM
                     VGYEWKLKPAPPKMRAELVIEQIRSLSEKLSLTPQLGGAQVVIVDPADAINWNAFNAL
                     LKTLEEPQPGRYLWLLSSNPARLPATIRSRCQRLDVRLPPHEEALAWLLSRGHAAKVA
                     GEALEAARGHPGLADAWASGDGLALRRAVADDARSLARGQAAVGEVAQRWVADGRASE
                     RLQHLADLALHEAAGLTDPAATRTLAARFDAANRARDLLRSTVRADLAVAESLLAWC"
     gene            10221..10583
                     /locus_tag="PK399_03270"
     CDS             10221..10583
                     /locus_tag="PK399_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPW12173.1"
                     /translation="MSSPMGGGARQGILSLVVKDKPSLYNAYMPYVRQGGIFVPTTRR
                     YFIGDEVFLLLTLPDSSERLPVAGKVCWVTPTGAQGSRPAGIGVQFADTAEGEAVRGK
                     IETTLAGTLNSDKPTQTM"
     gene            10583..11665
                     /locus_tag="PK399_03275"
     CDS             10583..11665
                     /locus_tag="PK399_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4380 domain-containing protein"
                     /protein_id="HPW12174.1"
                     /translation="MGTMPKRGIMPLRAWLPSSGLVLVLCLPIASAGDRDPDGSRLRI
                     EDAGLVVEVSPRLGGRMLHLSLPGAPNLLKVGDAVVAQPDPRVDAQADDIGYLGHQVW
                     LGPQSQWWTHQQANPVRSEARAPWPPDPWLSYAATAIVVQAADRMALRGPPSPVSGVR
                     MTHDYALPAGKPGTLVTTATMRNIRDTPVSWGLWFNSRLPADTRVYVPVAGDSDVRLR
                     HDVDAGFDGLSGATEGGFFTLDLDTPAAGKQGRRGKVFIQPRAGWMAGFRAGQAFVIR
                     FDLQPRDHIHAEHGQVELYLNWLPGDQAGSLLEMEVHGAYTTLAPDAEANAKETWIVR
                     RYDGPDGRNAQLAFLATMLAAAGIDP"
     gene            complement(11726..13801)
                     /locus_tag="PK399_03280"
     CDS             complement(11726..13801)
                     /locus_tag="PK399_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPW12175.1"
                     /translation="MADSPAAGFDGLRLRHWRPHRREDGVLVLQFDRADAPVNAFSQD
                     VLIELADVLERIAIDPPKGVVIASAKASGFIAGADLKEFQEFDRKGTVDDAIRRGQDV
                     FQRLAELPCPTVAAIHGFCMGGGTEIALACRFRVASNDASTRIGLPETQLGIFPGWGG
                     SARLPRLVGAPAAMDMMLTGRALSASAARGIGLVDKVVEPALLIDAAASMALRGTTRP
                     FKQRALAWATNTFAARKLLAPQMAKQVARKAKKDHYPAPYALISTWQRFGGKGLQARL
                     DGERRAVVKLAGTPTARNLIRIFFLTERLKALGKGDAGIRHVHVVGAGVMGGDIAAFA
                     AYKGFEVTLQDREQRFIDAAMTRAQVLFDKKVKDPTKRPAVAARLQPDLAGDGVAQAD
                     LVIEAIIENPDAKRTLYETVEPRLKPDALLTTNTSSIPLDELRGHIARPAQFAGLHYF
                     NPVAQMPLVEIIHHDGMAAETERRLAAFCKALGKFPVPVAGTPGFLVNRVLFPYMLEA
                     GTAYAEGIPGPAIDKAAVKFGMPMGPIELIDTVGLDVAAGVGRELAPFLGLQVPAALS
                     QVDPAKRGKKDGQGLYTWENGKAKKPELPADYKAPEDLEDRLILPLLNEAVACLHDGV
                     VADADLLDAGVIFGTGFAPFRGGPIQYIRSAGADALLGRLKALQAKYGERFAPRQGWD
                     NPALREPPQ"
     gene            13966..14580
                     /gene="rpoE"
                     /locus_tag="PK399_03285"
     CDS             13966..14580
                     /gene="rpoE"
                     /locus_tag="PK399_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007962205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HPW12176.1"
                     /translation="MAEDDVSQALDQDLVARVQRGDSAAFDLLVRKYQHRTAALISRY
                     IHDWSEVQDVAQDTFIRAYKAIGGFRGDAQFSTWLHRIAVNTAKNHLVSSNRKPPTDD
                     IEIEDAEQFESGLRLRDNDTPERELMRQQLEQTVLRAVEDLPPELRDAITFREVEGMS
                     YEEIAERMDCPIGTVRSRIFRAREAIDERMKPLLDHDERKRSHA"
     gene            14591..15457
                     /locus_tag="PK399_03290"
     CDS             14591..15457
                     /locus_tag="PK399_03290"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HPW12177.1"
                     /translation="MTQDTTLRANTLTQLSSREQLSALMDGALPPDETRFLLRRLQHD
                     ASLAECWQRWRLGGEVMRGLAPAQRLPADFATRVSAAIFDSGQGTVLGEPVERRAPKP
                     AWLRWGGGAAMAASLAVVVGFIARQPAVETPATPLQPQVAAAAAVADPAPAPAPALPE
                     PAVPSLPIASDTLVAATATAIAAASRPQRSRDTTRSARVLPAAVRQVESVAAASLPIN
                     VPQPDIVTRPWPRSVLPQYAGNPLAAGFGGHIRAEAPYNPFASPALADIPPPAVAQDA
                     PADAPAAADSRP"
     gene            15523..17064
                     /locus_tag="PK399_03295"
     CDS             15523..17064
                     /locus_tag="PK399_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HPW12178.1"
                     /translation="MTAAKTPLLRPLVLSVALAAAGLGVGSWYGVATAQSTVQAPAPE
                     LVSGLPDFTRLVDQVGPAVVSVQAEIGGKPQAVAQQPDPRQVPDDEEVPEFFRRFFGP
                     GMGPGMPGMPGAPGVPNARPRGVSQGTGFIISGDGYVLTNHHVVDGADTVRIKLSDRR
                     EFVAKVVGSDEQSDVALLKIDANGLPTLRIGDSRTLKSGQWVVAIGSPFGLDHSVTAG
                     IVSAVGRANPYADQRYVPFIQTDVAINRGNSGGPLIDTRGQVVGINSQIFSNSGGYMG
                     VSFAIPIDVAMNAVQQLKATGKVTRGQLGVQIQAMTRDAAKALGLGDANGALVARVEP
                     GSPAERAGILRQDVIRSINGQQVYESSDLPPIVGAMPPGTRVSIELVRDGKPRTVTAT
                     LNQLSEATAGPVVGDDGAPRPAAAAQANALGIVGEDLDPQQRSRLGLQAGEGVLVARV
                     EGVAAREAGLRPGDVILAVGRNDVSSAARLNAQLRAAGAGKPVMLLVRRGGGTQYLTI
                     NPTGD"
     gene            17197..18990
                     /gene="lepA"
                     /locus_tag="PK399_03300"
     CDS             17197..18990
                     /gene="lepA"
                     /locus_tag="PK399_03300"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPW12179.1"
                     /translation="MRNIRNFSIIAHIDHGKSTLADRIIQLCGGLQDREMEAQVLDSN
                     PIERERGITIKAQSVSLPYTALDGTTYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAAQGVEAQSVANCYTAVEQGLEVVPVLNKIDLPTADIDKVKEEIEAVIGIDAEDAVA
                     ISAKTGLNVRDVLEAIVKRIPPPKPRDTDKLQALIIDSWFDNYLGVVSLVRVMQGEIK
                     PGDKLLVMSTGRTHQVDKVGVFTPKRKELARLGAGEVGWVHASIKDVHGAPVGDTLTL
                     AGDPAPGPLPGFQEMQPRVFAGLFPVDAEDYPDLREALDKLRLNDAALRFEPESSEAM
                     GFGFRCGFLGMLHMEIVQERLEREYDLNLISTAPTVVYEVLKTDGGIVPMDNPAKLPP
                     INQVQEIREPIIRANILTPPDYIGNVIKLCEEKRGVQIGITYMASQVQVSYELPMAEV
                     VLDFFDKLKSVSRGYASLDYHFVRFEAGPFVRVDTLINGDKVDALSIIVHRSHADRRG
                     RELTEKMKELIPRQMFDVAIQAAIGAQVIARTTVKAMRKNVLAKCYGGDASRKKKLLE
                     KQKAGKKRMKQVGSVEIPQEAFLAVLQVDNK"
     gene            19022..19825
                     /gene="lepB"
                     /locus_tag="PK399_03305"
     CDS             19022..19825
                     /gene="lepB"
                     /locus_tag="PK399_03305"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPW12180.1"
                     /translation="MVWFETILVALTALTGLVWLLDKLFLAKRRAAAAGPLDEVKEPV
                     AVDYSKAFFPILLLVLGLRSFVAEPFRIPSSSMMPTLLIGDFILVNKFSYGLRWPITN
                     KKFLANGEPVRGDVVVFRPPHHPREDWIKRVIGLPGDTIGYHDNKLSVNGREVPYRVE
                     GAYQGEGQGAEMTGASRLTEGLPGRPHFVLRRDDTPLPYQGEGDWTVPEGHYFVMGDN
                     RNNSEDSRFWETHFLPEANLRGKAMIVWMNFDRQADHWVDTSRIGKRIP"
     gene            19856..20236
                     /locus_tag="PK399_03310"
     CDS             19856..20236
                     /locus_tag="PK399_03310"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HPW12181.1"
                     /translation="MKRMQRGMTMIGFLITLSVVILFVFCGMKVVPMYIEFYSVKKAL
                     ASIANEQDTSGATKDKIRAMFARHLTIDYVKVIKPEMLQIQTTDDGYNMIVEYERREE
                     LIANLDVVAKFRAEQALVRGAAAQ"
     gene            20217..20900
                     /gene="rnc"
                     /locus_tag="PK399_03315"
     CDS             20217..20900
                     /gene="rnc"
                     /locus_tag="PK399_03315"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPW12182.1"
                     /translation="MAQRRNDLAPRAFAGHAFADPALLDQALTHRSAGSPHNERLEFL
                     GDALVNQFIAEALYLHWPRADEGALTRGRAELVRESALAGIARTLDLGVHLVLGPGEM
                     KTGGHRRDSILADALEALVAAIYLDAGFDACRAVVLPWFDPLVAALPPANKVGKDAKT
                     RLQEWLQARQRGLPVYTLVEEQGDDHARVFRVSCRVAEPAITTEGEGSSRRAAEQQAA
                     EAAMQVIDP"
     gene            20897..21817
                     /gene="era"
                     /locus_tag="PK399_03320"
     CDS             20897..21817
                     /gene="era"
                     /locus_tag="PK399_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPW12183.1"
                     /translation="MSKPSQRSGAIAVIGRPNVGKSTLVNALVGAKISITSNRPQTTR
                     HRLLGIATFPGGQLQLVDTPGIHGEQGKRSGKAMNRMMNRAARGALEGVDAAVLVVEA
                     GRWDAEDGLAFDALREGGLPVVLVVNQVDKIKDKGTLLPFIAKVSEGREFAAVHPLSA
                     LKRKGLEALVDTLLGLVPEAAPMFGEDEITDKSQRFLAGELVREQLMRQLGEELPYAT
                     TVEVEKFEEDTTGRQLVYRIDAVVWVEREGQKAIVIGKGGERLREIGTRARGQMERLF
                     DSKVFLQTWVRVREGWSDDEAALRSLGYHD"
     assembly_gap    22054..22153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22164..22895
                     /gene="recO"
                     /locus_tag="PK399_03325"
     CDS             22164..22895
                     /gene="recO"
                     /locus_tag="PK399_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPW12184.1"
                     /translation="MRFVGEPAFVLHARAWRETSLLVEVLSAEHGRIGLVARGVQGPK
                     KHVLRAALQPLQSIRFDGVQRGELAQLTSAEAVDAAPLLHGDATLGGFYLNELCMRLA
                     PRQAPQPELHAAYARVRERLRAGEPLAWILRRFERDLLDALGSGFDYASDGEGAPIDP
                     AARYRLEAEHGARRVLGERAGERRESATGRALLALGGDSLPNGEDLASLRLPLRGVLL
                     HHLGGRGLKSWEMLGELGRLAAPKP"
     gene            complement(22892..23581)
                     /locus_tag="PK399_03330"
     CDS             complement(22892..23581)
                     /locus_tag="PK399_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW12185.1"
                     /translation="MNPATSSPLPRLLLVEDDPVSAAFLCDAASGLPAVVQLAGSLAE
                     ARRAIAAMAFDLWLVDANLPDGPGEILLEATTGAAAALAHTADDNAATRERLLAAGFL
                     EVLCKPISVTRLHAALRRHLPMPVHRVGDADLPDWNDAGAQAALGDAAHVDVLRQLFR
                     REMPAQRARIAAAMGDAATMQADLHRLAASCGFVGADRLRDAVRRLMDDPTDPAAMQA
                     FTAAADALIEG"
     gene            23627..25645
                     /locus_tag="PK399_03335"
     CDS             23627..25645
                     /locus_tag="PK399_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12186.1"
                     /translation="MLTACSLLAMLAAAPVHARAAQVRIARVATPVATLEQVRVRLDW
                     PEGAATGELQVWAGKVDAADLGYRYRDLHWRCPLRRAAHDGWQCDGALRAGMAAPLRL
                     AVQFDAATTQASLAKGDARFTLDRSAATPDLTSLDLTRVPMAWAQALLAQAWPAARLT
                     GGQLDASLAIEAPANRPLRISGQLRPRGVGMETADATIAGENLGGDLRIDYRTTPARS
                     WLALLGELRGGEFLAGSTYVALPATAVGLRIDAMKQAGSGWQVPRIEWRDGEALQANG
                     TLAFDKDGALSSMAVDAGSGDMSPLRQRYLSGWMGLFGLGDVDLHGAMDLHVEAQGGG
                     LSRIDAILHGVDLRDPARRFVFDGLDGDLRYSASAPVRSELRWSGGQLYGLAFGAARL
                     PMASADGQLRFGEDVHVPLMGGQLTLRDVVIQPPRDAAGMQIRFGLGLDDIDFGKVSQ
                     ALGLPAFQGRLSGRIPQARYADERIEFDGGLSLRLFDGRVAFSALSLERPFGSAPSLS
                     ADIAFDGLDLLRLTEVLGFGSITGRLDGRVEGLRLVDWTPVAFDARFITDEAPGVKQR
                     ISQRAVQNISSVGDASFVTSLQGQLIGLFDDFGYRRIGIGCRLANEVCAMSGLDSHRS
                     QGGNAFTIVEGAGLPRLDVVGFNRNVDWPTLVERLVAVGKGEVAPVVE"
     gene            25680..26300
                     /locus_tag="PK399_03340"
     CDS             25680..26300
                     /locus_tag="PK399_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HPW12187.1"
                     /translation="MKQWLGMPVAGALLLSACVTINVYFPAAEAKEAAREFVEKVINE
                     ADKVEMKDPSAPAGGMASLGTRIRSDLSGFDPWVLVGIGSAQAQSAPDISLKTPAIQA
                     IQSRMEARFNATLRGGFDSGALGFTADGLVTVRDAGKLELKDRVAMNAAVADDNRDRK
                     AVYREVAVANGHPEWETQIRDVFARQWIASARPGWWYQSGGSWKQK"
     gene            26398..>26923
                     /locus_tag="PK399_03345"
     CDS             26398..>26923
                     /locus_tag="PK399_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase"
                     /protein_id="HPW12188.1"
                     /translation="MARLDHSPKSLDIVNLTHDGRGVARWPDGPHAGKTVFVTGALPG
                     ETVLVQQTARSRNFDEAKTLEVVQASPDRVTPRCPHFGTCGGCVLQHLAEDKQIEAKQ
                     GVLLDNLQRIGHVTPQSVLPPLRGDSWGYRRKGRFSVRRVEKKDKTLVGFREQDPRFV
                     ADIGECHVVVPQLGF"
BASE COUNT         4315 a         8863 c         9350 g         4195 t
ORIGIN      
        1 atccatttca gatttggagt tgcaccatgg ccgtccagaa gtcccgcgtt tccccgtccc
       61 gtcgtggcat gcgccgtgcg catgacgcgc tgtccgccaa gcagttggcc accgacccga
      121 ccagcggcga gacccacttg cgccaccacg tgaccgccga cggctactac cgcggcaagc
      181 aggtcatcgc gccgaagacc aaggtcgccg acgaagagtg atccgcggcc gcaaggtcgt
      241 gtttgctgga tgacgcccgg ccgcgccacg tgcgcggccg ttggcgtttc tgtggtgggt
      301 gttcgctgat tcccatccgc aggcggtacg gatgccgcat cccgttggcc gcacgcgcta
      361 tggtgggtgg ccttccaact ccgtttcgcc aggaccgcat gcaatgagcg atagccaagg
      421 ccgcgtctat tcccgtatcg caggcaccgg cagctacctg ccggacaagg tgctgaccaa
      481 cgccgacctc accgagttcg tcgagaccag cgacgaatgg atcgtggccc gcaccggcat
      541 ccgcgagcgg catgtcgcgg ccgagggcga gaccacctcc gacctcggtt accaggccgc
      601 attgcgcgcg atggaggccg cgggcgtgac cgccgccgag atcgacctga tcatcctggg
      661 caccaccacg cccgacctga tcttcccgtc gtcggcctgc ctgctgcagc acaagctggg
      721 cgcgaacggc tgcccggcct tcgacgtcaa cgcggcctgt tccggcttca tctatgcgct
      781 gaccatcgcc gacaagttca tccagtcggg cgcggcgaag acggcgctgg tggtgggtgc
      841 ggaaaccctg acccggatgc tggactggaa cgatcgcggc acctgcgtgc tgttcggcga
      901 tggcgccggc gccgtggtgc tcaaggcgga tgccgaaacc ggcatcctca gcacccacat
      961 gcatgccgac ggcggcaaga aggaactgct gtggaacccg gttggcgtct cggtcgggtt
     1021 caagccggac gagaagaacg ccggcgtgcg cgtgctgatg accggcaacg aggtgttcaa
     1081 gcatgcggtg aaggcgctgg actcggtggt cgaggaagcg ctggacgcga acggcatgga
     1141 tcgccacgac atcgactggc tggtcccgca ccaggccaac ctgcgcatca tcgaagccac
     1201 ggccaagcgc ctggacatgc caatggaccg cgtcatcgtc accgtggatc gccacggcaa
     1261 tacctcgtcc ggttcggtgc cgttggcgct cgacgaggcg gtgcgctccg gcaagatcca
     1321 gcgcggccag gtggtcctgc tggaggcctt cggcggcggc ttcacctggg gctcggcgct
     1381 gctgcgctac tgagcacacc tgcaagcggg cgttcgtgcg gcctgtgcga ccgcgagcag
     1441 gccatgggtg agcacatgcc gattgcgcag catgccagcg cgggatgtgc gaacgcccgc
     1501 atcgctgatg cgggccggtc gcgaagggcc tgcaacgatg cgtcgaacag gatgtccgca
     1561 cgcaccggga cggcgcactg gcggcgcggg cgactgccgc gagcaacccc aatacgcccg
     1621 ggtacagacg ggctagagca attgctcgta tcatgcgcat cccttgattc catgggcgtt
     1681 cccgcgcccg atcccgcgtg agcgaagccg ccacacaact cgccttggtc ttccccggcc
     1741 agggctcgca atcggtcggg atgctggccg aactctcgga actgcatccc ggcatgaagg
     1801 cgaccttcga ggaagcctcg gccggtgccg gcgtcgacct gtgggcgctg tcgcagggcg
     1861 gcccggaaga actgctcaac cgcaccgaat acacccagcc ggcattgctt gccgcagggg
     1921 tggcggtgtg gcgtgcatgg caagccagcg gtggtccggc gccggacgta cttgctggcc
     1981 acagcttggg cgagtacacc gcgctggtcg cggccggagc gctgtcgctg catgacggtg
     2041 cccacctggt gcggatccgc gggcagttga tgcaggacgc cgcgcctgcc ggaaccggtg
     2101 cgatggcggc catcctgggc gccgatgacg cgctggtggc cgaggtgtgc gaagccgcct
     2161 ccggcaccca agtcgtggtc ccggccaatt tcaactcgcc agggcagatc gtgatcggtg
     2221 gccatgcggc tgcggtcgat gccgcgctgg cgatgctggc cgaacgtggc gtgcgcaagg
     2281 cgatcaagct ggcggtgagc gtgccttcgc atacgccgct gatgcgcgac gccgccaacc
     2341 ggctggccga gaccatggcc ggcctgcagt ggcggatgcc gtcgctgccg gtggtgcaga
     2401 acgttgacgc gaccgtgcac gacagcgtgg acgcgatccg cgatgcgctg gtgcgccagc
     2461 tttacctgcc ggtccagtgg acgggctgcg tgcaggcgct ggccgcgcgc ggcgtcggcc
     2521 ggatcggcga atgcgggccg ggcaaggtgt tggcnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnntggccg cgcgcggcgt cggccggatc ggcgaatgcg ggccgggcaa
     2701 ggtgttggct ggcctggtca agcgcatcga caagtcgctc gatgcacgtg cattgggcac
     2761 gccggctgaa ttcgccggcg ccatcgagga atggaacgca tgagtgagct tgtcctgaaa
     2821 ggcgaagtcg cactggtgac cggcgcgtcg cgcgggatcg gcgcggcgat tgccgacgaa
     2881 ctggccgcgc tgggcgcaac cgtcatcggt accgcgacct cggatgccgg cgcgcaagcg
     2941 atcggcgagc ggttagcggc ggcgggtggc catggccgca ggctcaacgt caacgagacc
     3001 ggcagcgtag aggcgttgat cgacgcggtg tccaccgaat tcggtggtgt ctcgatcctc
     3061 gtcaacaacg ccggcatcac ccgcgacaac ctgctgatgc ggatgaagga cgaggactgg
     3121 caggccatcc tcgacaccaa cctgacgtcg gtctatcgcg catcgaaggc ggtgatgcgc
     3181 gggatgatga aggcgcgcaa gggccggatc atcaacatcg cctccgtcat cggggtgacc
     3241 ggcaatgccg ggcagtcgaa ctacgccgcc gcgaaggccg ggatcatcgc cttcagcaag
     3301 tcgctggcga aggaaatcgg ctcgcgcggg atcacggtga atgtcgtcgc cccggggttc
     3361 atcgccaccg acatgaccgc cgacctgccg gaggccgcca aggaggcgat gctcggccag
     3421 atcgcacttg gcaggctcgg cgaaccggcc gacatcgccc gcgcggtggc cttcctggcc
     3481 ggcccgggcg ccacctacat caccggcgaa accctgcacg tcaacggcgg gatgtacatg
     3541 ccgtagcatt cgcggcgtgg gtgccgcggg cggccccatc gccatggatc catccgggaa
     3601 cgccatccgg ctttctcgta cacttagcca cgaaaccgcc ccctgaggag caccgagaca
     3661 tgagcaacat cgaagaacgc gtcaagaaga tcgtcatcga acaactgggc gtcaaggaag
     3721 aggacgtcag caacagcgcc tcgttcgtcg aggacctcgg cgcggactcg ctggacacgg
     3781 tcgagctggt gatggcgctg gaagaagagt tcgagtgcga gatcccggac gaagaagccg
     3841 agaagatcag caccgtgcag gcggcgatcg actacatcaa ggcccacgtc aaggcgtaat
     3901 tcgcccgtgt gagtcgccac gccggggccg ctgacgcggc cccgtgcgtt ttcaccaccg
     3961 caacatcagc accaccgcaa catcggacaa agatcgagga aacgccatgt cgaagcgtcg
     4021 cgtcgtcgtc acgggaatgg gtatcgtctc gccgctgggc aatgacctgg ccagcaactg
     4081 ggacggcatc gtcaacggcc gttccggcat cggcccggtc gcgggtttcg atgccagcac
     4141 ctatccgacg cgcatcgccg gcgagatccg cgacttcgac gtcacccgct gggtgaaccc
     4201 gaaggacgcg aagaagatgg accacttcat ccattacggc gtggcggcct cgctgatggc
     4261 gctggaggat tccgggctgc aggtgaccga agccaacgcc gagcgcgtcg gtgcgctgat
     4321 cggttccggc atcggcggca tctgggggat cgaggagacc tcggtgaagc tgcacgaagg
     4381 cggggtgcgc aagatcagcc cgttctacat ccccagcacg atcatcaaca tgctaccggg
     4441 acaggtgtcg ctgctgaccg gcatcaaggg cccgaatttt tccgccgtgt ccgcctgcgc
     4501 caccgccaac cattcgatcg ggatggcgat gcggatgatc cagcatggcg atgccgacgt
     4561 gatgatcgcc ggcggtgccg aacgcggcag ctcgccgacc tcgatgggcg gcttctgcgc
     4621 gatgaaggcg atgagcacgc gcaacgacga tcccgcggcc gcttcgcgcc cgtgggatgc
     4681 aggccgcgac ggcttcgtgc tgggcgacgg cgccggcatc ctggtgctgg aggaatacga
     4741 acacgccaag gcacgcggcg cgcgcatcta ctgcgagctc gccggcttcg gcgcgtcctc
     4801 cgatgcattc cacatgaccg cgcccagcga gaacggcgac ggacccgcgc gctgcatggc
     4861 gatggccttc cgcgacgccg gtatcaacgc ggacgaggtc ggttacctca atgcgcacgg
     4921 gacttcgacg ccgctcggcg accttgcgga aacgctggcg atcaagcgcg cgctcggcga
     4981 ccacgcgtac aagacgatgg tcagttcgac caagtcgatg accggccacc tgctcggcgc
     5041 cgccggcggc gtggaggcga tctacacgat caaggcgctg gagaccggga tcatcccgcc
     5101 gacgatcaac ctggaaacgc cgagcgaagg ttgcgacctg gactacgtgc cgaacgtcgc
     5161 gcgcgaagcc agggtcgacg tggtggtgtc gaatgggttc ggcttcggcg gtaccaacgg
     5221 caccttggta ttccgtcgca tctgatgccg atgtcctccc ttgcgtgcag gggagggcgt
     5281 cgatggggtg ctctgtgccg atgatcaccc gaacccttcc cgccgacgtc gacctgctcg
     5341 acctgcatcg cctggatccg gcgcgttacc cggtgctgct ggaatccagc gcgaccggtg
     5401 cgcacggtcg ctgggacatg ctgctggcgc acgacggcga atcgttcgtg ctgcatcgcg
     5461 acggcatggt ccgcgaccag gatggcgggt ggatcgacgg gcgcttcctc gacgtgctgg
     5521 atgcccgctg gcaggcgcaa cgcctgccgc gcgccgaagt cgatacgttg ccgttccgcg
     5581 gcggctgggc gcttttgctg ggatacgagc tggcgcaaca ggtcgagccc gtgctgcggc
     5641 tgccgcctgc cgcgggtggt ttgccgatcg cctgcgccct gcgttgcccg gctgccgtgt
     5701 tgcgggatcg cgtgaccggc gcgtgcttcg ccgtggccga agccgatgcc agcgcgtggc
     5761 tcggacgcat tggcgccgac cttgcgcgtg cgggctcagc gggatcgctg ccggactggc
     5821 aggcgcctgc agggatcgcg gaagagccat cgcagcgcta tgtcgatggc gtgcaacggg
     5881 tgctggggta cctcgctgcg ggcgacgtgt tccaggtcaa tctttcgcgt ggctggacgg
     5941 cgcgtttcga ttcgccgctt gcacccgcag ccctgttcca gcgcctgcgc ggcaacaatc
     6001 ccgcgccctt cgccggcatc tttcgcaccg acgcgggcag cgtggtcagc gcctcgccgg
     6061 agcggctggt gtcggtgcgc ggcgacgtcg tcgagacccg tccgatcgcc ggtacccgcc
     6121 cgcgtttcgc cggggatgac gacggtgaac gcatccgcga actcgttggg catccgaagg
     6181 agcgcgccga acacgtgatg ctgatcgacc tggagcgcaa tgacctgggt cgcgtctgca
     6241 cgccgggcag cgtggtggtc gacgagctga tgacggtcga gagctatgcg cacgtgcacc
     6301 atatcgtcag caacgtgcgt ggcaccctgt tgccggatgc aagcccgggg caggtgatcg
     6361 cggcagtgtt tcccggaggc acgatcaccg gctgcccgaa ggtgcgctgc atgcagatcg
     6421 tcgccgagct tgaaggcgcc gggcgcggcg cttataccgg cgcgatgggc tggctcaatc
     6481 gcgacggcga catggacctg aacatcctga tccgcagcgg cgaagtggaa ggccaggagc
     6541 tgcgcttccg caccggcgcc ggcatcgtca tcgactccga tccgcagcgc gagctggacg
     6601 agacccgggc caaggcgcgt gggatgctgc gtgcattggg tgtcgaatga tcgcggtggc
     6661 ctacatcggt cgcgaaccga tggatgcggt ttcgatcggc aatcgcggcc tgaactacgg
     6721 cgatggcgtg ttcgagacca tgcgcgtgca tcgcggcgtg ctgccgctgt ggccacgcca
     6781 cctggcgcgg ctgcgcgagg gcgcggggcg gctcgggatc gcaatgccgg atgtgggcgt
     6841 catggaagcc cgcattgccg aactcatggc gggcgcagat gccggcgtgc tcaagctgct
     6901 gctgacccgc ggcgaaggcg ggcgcggtta tgcgccgcca ctcgacgcag tgccgacctg
     6961 gatgctgtcg ctgctgccat tgccggcccc acagggtgcg ttgcggctgc atgcctgcga
     7021 ggtgcgcctg gcgatgcagc ctgcgctggc cggcatcaag cactgcaatc gcctggaaca
     7081 ggtgctcggc cgtgccgagg ccgagcgcgc gggctgcgat gaaggcctga tgcgcgacat
     7141 ggtgggcaac cccgtctgtg cgacctcggc caacctgctg gtattgcgcg acggccgctg
     7201 gacgacaccg ccggtcgacc tgtgtggcgt cgccggcgtg ctgcgtggct ggctgctgga
     7261 ccaaggcctg gtcgcgttgg cgtcgctgtc gatggacgat gtcgaatcgg cccaggcctt
     7321 ggcgctgtgc aacgcggtgc gcggtatcct gccggtgcgc gcactgggtg cgcgtgaatg
     7381 gccaacgcat cccgcagtgg ccgacctgca gaagcgattg gcgatggcga ctccgatgtt
     7441 ctccgaatcg cgggagaccg catgaaaccg cgtgttgccg gtggtgcctt cgcgcgcttc
     7501 ctgctggtcc tgctgctggc gggcctggcc gttgcgggct ggcagtggca acgctaccag
     7561 gggttcgcgg atgcgccgct ggccggcatc gacgccggcg acagcctggt ggtcgcgcgc
     7621 ggggattcgc tcgacaaggt attgcgcacg ctgcaggcgc agggcgtcga caccggcgac
     7681 cgcgtgcaat ggcaggtact ggcgcggcag accggtgcgg cgggcaagct gcaggtcggg
     7741 gaatacgcgc ttgcccccga cacatcgccg cgcgcgctgc tgctggcgat gcgtgacggc
     7801 aaggtggtgc ggcgcaactt caccatcgtc gaaggctgga atatccgcga cgtgcgggcc
     7861 gcgctggcga aggtcgaggg gctcgagcac gattcggcgg ggctggacga tgccgcgttg
     7921 atgaaggcat tgggccatcc cggccagcat cccgaaggac ggttcctgcc ggagacctac
     7981 gcctgggtga aaggcgacag cgacctcgac ctgctcaagc gtgcgcacaa ggcgatgcaa
     8041 gaggcactgg acgaggcctg ggcatcgcgc gcggacgacc tgccgctgaa gaatgccgac
     8101 gaagccctcg tgctggcctc gatcgtggaa aaggaaaccg gcatcgccga ggaacggccc
     8161 gcgattgccg gggtgttcgt gcgtcggctg cagatcggca tgcgcctgca gaccgatccc
     8221 accgtgatct acgggatggg cgcggcctat gccggcaaca tccgccgcag cgacctgacc
     8281 accgatacgc cctacaacac ctacacccgc gccggcctga cgccgacccc catcgcgatg
     8341 ccgggcaagg ccgcgttgcg cgcagcgacg cggccggccg atggcgacgc gctgtacttc
     8401 gtcgcggtgg gcgatggcag cggccggcat gtgttctcgg cctcgctgga tgctcacaac
     8461 gcggcggtcc gcgactacgt gcagcgctac cgcgcgcagc gccaggccgc ccccaagtga
     8521 ccgcggcgct gcaacgccag ccgcgcctgc tgagcatcga aggcggcgaa ggtgccggca
     8581 agagcacggt cctttccgcc ctgcgcgatg cgctgctggc cgatggccag gaggtcgtca
     8641 gcacccgcga accgggcggc acgccactgg cggaacgcat ccgcgaactg ctgctgggcg
     8701 gcaccggcga aaccgtgcat ccgcagacgg aattgctgct gatgttcgcc tcgcgcgcgc
     8761 agcatgtgcg cgaggtcgtg ctgccggcgc tgcaacgcgg tgcatgggtc atcagcgacc
     8821 gcttcaccga ttccagttat gcctaccagg gcgccgggcg cggactggac gaaggctaca
     8881 tcgccgcgct ggaacagcag gcggtcggca tcgagccggg cctgaccctg ctgctggacc
     8941 tgggcgtggc ccatggtcgc gagcgcaccc gcgggcgcga cctgctgggc ggcagcgtgc
     9001 cggatcgcat cgagcgcgag cgcgacagct tcttcgagcg cgtgcgcgac ggcttcctgg
     9061 cccgcgcggc gcagcatccg gatcgcttcc gggtgatcga tgccagccac gacgcggcga
     9121 gcgtggccgc cgcggcactc gcggcgctgg ccgactatct cgggacgctg cggtgagcct
     9181 gttgccgagc ttgagcccgt ggcagcaacg cgcctatgac catgcgaccg cggcgatcga
     9241 tggtggccat ttcggccatg ccaccctgat caccggtccc gcgttgatcg gcaaacgcgt
     9301 gctggcggaa cacctggcgc accgcgtgct gtgccgcagt ccgcaggcga atggcgaacc
     9361 ctgcggcgcg tgcaagggct gctcgctgtt cgccgcgcgc gcgcagtacg acccgctgga
     9421 agttcgcccc gacggtaccc cttcgcatcc gtggggccat agcgcgcatc ccgatttgct
     9481 gatggtcggt tacgagtgga agttgaagcc ggcgccgccg aagatgcgtg ccgagctggt
     9541 catcgaacag atccgctcgc tgtccgagaa gctctcgctc acgccgcaac tgggcggcgc
     9601 gcaggtggtc atcgtcgacc ccgccgacgc gatcaactgg aatgccttca acgccctgct
     9661 gaagacgctg gaggagccgc agccgggccg ctacctgtgg ctgctgtcgt ccaatccggc
     9721 gcgcctgccg gcgaccatcc gcagccgttg ccaacggctg gacgtgcgcc tgccaccgca
     9781 cgaggaagcg ttggcgtggc tgctgtcgcg cgggcacgca gcgaaggtgg cgggcgaggc
     9841 gctggaagcc gcgcgcgggc atcccggcct cgccgatgcg tgggcatcgg gcgatggact
     9901 ggccctgcgt cgcgcggtgg ccgacgacgc acgttcgctc gctcgcggcc aggccgcggt
     9961 gggcgaggtc gcgcagcgct gggtcgccga tggccgtgcc agcgagcgcc tgcagcacct
    10021 ggcggacctc gccctgcacg aggccgcggg cttgaccgat ccggccgcaa cccgcacgct
    10081 ggccgcgcgg ttcgacgccg ccaaccgcgc gcgcgacctg ttgcgcagca cggtgcgcgc
    10141 cgaccttgca gtagccgaat cattgcttgc ctggtgctga tgtccaacgc acgcccggca
    10201 tcgcgaacgg gggatgacgc atgagcagtc cgatgggtgg tggtgcacgg cagggcatcc
    10261 tgtcgctggt ggtcaaggac aagccgtcgc tctacaacgc ctacatgccc tacgtgcgcc
    10321 agggcggcat cttcgtgccg accacccgcc ggtatttcat cggcgatgag gtcttcctgc
    10381 tgctgacgtt gccggattcg tccgagcgcc tgccggtggc cggcaaggtg tgctgggtga
    10441 ccccgaccgg cgcgcagggc agccgccccg ccggcatcgg cgtgcagttc gccgacaccg
    10501 ccgagggcga ggccgtgcgc ggcaagatcg aaaccacgct ggccggcacg ctgaactcgg
    10561 acaagccgac gcagacgatg taatgggcac catgccgaag cgcgggatca tgccgcttcg
    10621 cgcgtggttg ccgtcatccg gcctggtgct tgtcctgtgc ctgccgatcg catcggcggg
    10681 cgatcgggat cccgatggtt cgcggctgcg gatcgaggat gcagggcttg tcgtcgaggt
    10741 ttcgccgcgg ctcggcggcc gcatgttgca tctctcgttg cccggcgcgc cgaacctgct
    10801 gaaagtgggc gatgcggtgg tcgcgcagcc cgatccgcgc gtcgatgcgc aggccgacga
    10861 tatcggctac ctcggccacc aggtctggct gggcccgcaa tcgcaatggt ggacgcacca
    10921 acaggccaac ccggttcgca gcgaagcgcg cgcaccatgg ccgccggatc cgtggctgtc
    10981 ctatgccgcg accgcgatcg ttgtccaggc ggcggaccgc atggcactgc gcggcccgcc
    11041 cagtccggtc agcggcgtgc gcatgacgca cgactacgcg ttgccggccg gcaagccggg
    11101 caccctggta acgaccgcga cgatgcgcaa catccgcgac acgccggtga gctggggcct
    11161 gtggttcaac agccggttgc cggccgacac gcgcgtctac gtgcccgtgg caggcgacag
    11221 cgatgtgcgg cttcgccacg atgtcgatgc cggtttcgac gggctctcgg gcgcgactga
    11281 agggggattc ttcaccctcg atctcgacac ccctgccgcc ggcaagcagg gcaggcgcgg
    11341 caaggtcttc atccagccgc gcgccggctg gatggccggg tttcgcgccg gccaggcctt
    11401 cgtgatccgc ttcgacctgc agccgcgcga ccacatccat gccgagcacg gccaggtcga
    11461 gttgtacctg aactggctgc ctggcgatca ggccggcagt ctgctcgaga tggaagtgca
    11521 tggcgcttac accacgctcg cgcccgatgc cgaagccaac gcgaaggaga cctggatcgt
    11581 gcgccgctac gatggtccgg atggtcgcaa cgcacaactg gcgttcctgg cgaccatgct
    11641 ggccgcggcc ggcatcgacc cctgatctcg ccgcacgaaa aaggcgccga agcgccctgt
    11701 tcgtgcgtcg cgattcaagc ggtcgttact gcggcggttc gcgcaaggcc ggattgtccc
    11761 aaccttggcg cggggcgaaa cgctcgccgt atttcgcttg cagggccttg agtcgtccca
    11821 gcaatgcatc cgcgccggcg ctgcggatgt actggatcgg tccgccgcgg aagggggcga
    11881 agccggtgcc gaagatcacg ccggcatcca gcaggtcggc atcggccacc acgccatcgt
    11941 gcaggcaggc caccgcttcg ttgagcagcg gcaggatcag tcggtcttcc aggtcctcgg
    12001 gtgccttgta gtcggccgga agctcgggct tcttcgcctt gccgttctcc caggtgtaga
    12061 ggccctggcc gtccttcttg ccgcgcttgg cgggatcgac ctgcgacagc gcggccggca
    12121 cttgcaggcc aaggaacggc gccagttcgc gacccacgcc ggcggccacg tccaggccca
    12181 cggtgtcgat cagttcgatc gggcccatcg gcatgccgaa cttcaccgcc gccttgtcga
    12241 tcgccgggcc ggggatgccc tcggcatagg cggtgccggc ctccagcatg tacgggaaca
    12301 gcacgcggtt gaccaggaag ccgggcgtgc cggccaccgg caccgggaac ttgcccaagg
    12361 ccttgcagaa tgcggcgagc cggcgctcgg tctccgcggc catgccgtcg tggtggatga
    12421 tctcgaccag cggcatctgc gcgaccgggt tgaagtagtg caggcctgcg aactgcgcgg
    12481 gccgcgcgat gtgcccgcgc aattcgtcga gcgggatcga cgaggtattg gtggtcagca
    12541 gcgcatcggg cttcagccgc ggctcgacgg tttcgtacag cgtgcgcttg gcgtcgggat
    12601 tctcgatgat cgcctcgatc accaggtcgg cctgcgcaac gccatcgccg gccaggtcgg
    12661 gctgcaggcg tgcggccacc gcgggccgct tggtgggatc cttcactttc ttgtcgaaca
    12721 gcacctgcgc acgggtcatc gcggcatcga tgaaacgctg ttcgcgatcc tgcagggtga
    12781 cctcgaagcc cttgtaggcg gcgaacgcgg cgatgtcgcc gcccatcacc ccggcgccga
    12841 ccacatgcac gtggcggatc ccggcatcgc ccttgcccag cgccttcaac cgttcggtca
    12901 ggaagaagat gcggatcagg ttgcgcgcgg tcggcgtgcc ggccagcttg acgacggcgc
    12961 ggcgttcgcc atcgagccgc gcctgcaggc ccttgccgcc gaaacgctgc caggtggaga
    13021 tcagcgcata cggcgccggg taatgatcct tcttcgcctt gcgcgccacc tgcttggcca
    13081 tctgcggtgc cagcagcttg cgtgcggcaa acgtgttggt ggcccaggca agcgcacgct
    13141 gcttgaacgg acgcgtggtc ccacgcagcg ccatcgacgc cgcggcatcg atcagcaatg
    13201 ccggttcgac caccttgtcg accaggccga tcccgcgcgc ggcactcgcc gacaacgcgc
    13261 ggccggtgag catcatgtcc atcgccgccg gcgcaccgac caggcgaggc aggcgggcgc
    13321 taccgcccca gcccgggaag atgccgagtt gggtttccgg caggccgatc cgggtcgacg
    13381 catcgttgct ggccacgcgg aagcggcagg cgagggcgat ttcggtgccg ccgcccatgc
    13441 agaagccgtg gatcgcggcg accgtggggc agggcagttc ggccagtcgc tggaacacgt
    13501 cctggccgcg tcggatcgca tcgtccacgg tgcccttgcg gtcgaattcc tggaattcct
    13561 tgaggtcggc gccggcaatg aagccgctgg ccttggctga ggcgatcacg acgcccttcg
    13621 gcgggtcgat cgcgatgcgt tccagcacgt cggccagttc gatcagcacg tcctgcgaga
    13681 acgcgttgac cggcgcgtcg gcacggtcga actgcagcac cagcacgccg tcctcgcggc
    13741 gatgcgggcg ccagtggcgg aggcggagcc cgtcgaagcc tgcggcgggg gagtcggcca
    13801 tgcgcgtacc atccatacca gtatgtgaag gcgcatccta gcccggcccg gcttgaatac
    13861 ctgtcaaatc gccttcaggt aaaagcactg gcgcggaact tccgtcgatc atcgctgtca
    13921 caaggcccgg acgcgaccgg gctcaccaac gaggaccggc gcgcaatggc cgaggacgac
    13981 gtgtcgcaag cgctggacca ggatctcgtc gcccgcgtgc aacgcgggga cagcgccgcc
    14041 ttcgacctgc tggtgcgcaa gtaccagcat cgaaccgctg cgctcatttc gcgctatatc
    14101 cacgactggt cggaagtcca ggacgtggcg caggacacct tcatccgcgc gtacaaggcc
    14161 atcggcggct tccgcggcga cgcgcaattc tccacctggc tgcaccggat cgcggtgaac
    14221 accgcgaaga accacctggt gtcgagcaac cgcaagccgc cgaccgacga catcgagatc
    14281 gaggatgccg agcagttcga gtccggcctg cgcctgcgcg acaacgacac gcccgaacgc
    14341 gaactgatgc gccagcaact ggaacaaacg gtgctgcgcg cggtcgaaga cctgccgccg
    14401 gaactgcgcg atgcgatcac cttccgcgaa gtggaaggca tgagctacga agagatcgcg
    14461 gagcgcatgg actgcccgat cggtacggtg cgttcgcgca tcttccgcgc ccgcgaggcg
    14521 atcgacgagc gcatgaaacc cctgctggac cacgacgaac gcaagcgttc gcacgcctga
    14581 gcccgccgcc atgacccaag acaccaccct gcgcgccaat accctgaccc agctgagcag
    14641 ccgcgagcag ttgtccgcat tgatggacgg cgccttgccg cccgatgaaa cccgtttcct
    14701 gctgcgccgc ctgcaacacg atgcgtcgct ggccgaatgc tggcagcgat ggcgattggg
    14761 cggcgaagtg atgcgcggac tggcgcctgc gcagcggctg ccggcggatt tcgcaacgcg
    14821 cgtctctgcg gcaattttcg attcgggtca aggcaccgtt cttggtgagc ctgtcgaacg
    14881 acgcgctccc aagcctgcgt ggttgcgctg gggcggtggc gcggcgatgg cggcatcgct
    14941 ggccgtggtg gtgggcttca tcgcccgtca gcctgctgtc gagacgcctg caaccccgct
    15001 gcagccgcag gtcgccgcgg ctgcggccgt ggccgatccc gctcccgcgc cggcacccgc
    15061 gttgcccgag cccgcagtcc cctcgctgcc gatcgccagc gacaccttgg tcgcggccac
    15121 cgcgacggcc atcgccgccg catcgcgccc gcagcgcagc cgcgacacca cgcgtagcgc
    15181 gcgggtcttg ccggcagcgg tccgccaggt cgagtccgtc gccgccgcca gtcttccgat
    15241 caacgtcccg caacccgata tcgtgacccg cccttggccg cgctcggtgt tgccgcagta
    15301 cgccggcaat ccgctggctg cgggtttcgg tgggcacatc cgcgccgaag cgccgtacaa
    15361 cccctttgcg tcgccggctt tggccgatat cccgccgcca gccgtcgcgc aggatgcgcc
    15421 cgccgatgcg cctgcggcag ccgacagccg tccctgagcg gcacgcgcac ggcccgatcc
    15481 aaccgacctg attccattca cgcgaacgag gattcgtcga cgatgactgc tgccaagact
    15541 ccgctgttgc gcccgctggt tctctccgtc gccctggccg ccgccggtct cggcgtgggg
    15601 agctggtacg gcgtggccac cgcccaatcg acggtgcagg caccggcccc cgagctggtc
    15661 tccggcctgc ccgacttcac ccggctggtg gaccaggtcg ggccggcggt ggtgagcgtg
    15721 caggcggaaa tcggcggcaa gccgcaggcc gtggcccagc aacccgaccc gcggcaggtg
    15781 ccggacgacg aggaggtgcc ggagttcttc cgccgcttct tcgggcccgg catgggcccg
    15841 ggcatgccgg ggatgcccgg cgcaccgggt gtgccgaacg cgcgtccgcg cggcgtgtcg
    15901 cagggcaccg gcttcatcat ttccggcgac ggttacgtgc tgaccaacca tcacgtggtc
    15961 gatggtgcgg acaccgtgcg catcaagctg tccgaccggc gcgagttcgt ggccaaggtg
    16021 gtgggtagcg acgagcaatc cgacgtcgcc ctgctgaaga tcgacgcgaa cgggttgccg
    16081 acgctgcgca tcggcgactc gcgcacgctc aagtccggcc agtgggtggt cgcgatcggc
    16141 tcgccgttcg gactggacca ttccgtgaca gccggcatcg tcagcgcggt cggtcgcgcc
    16201 aatccgtacg cggaccagcg ctatgtgccg ttcatccaga ccgatgtggc gatcaatcgc
    16261 ggcaattcgg gcggaccgct gatcgataca cggggccagg tggtcggcat caactcgcag
    16321 attttcagca attccggcgg ctacatgggg gtcagcttcg cgatcccgat cgacgtggcg
    16381 atgaacgcgg tgcagcagct caaggcgacc ggcaaggtga cgcgcgggca attgggcgtg
    16441 cagatccagg cgatgacccg agatgctgcg aaagcgctcg gcctaggtga tgcgaacggc
    16501 gcattggtgg cgcgcgtgga gccgggcagt ccagcggagc gcgctggcat cctccgccag
    16561 gacgtgatcc gcagtatcaa cggccagcag gtctacgagt ccagcgactt gccgccgatc
    16621 gtcggcgcga tgccgcccgg cacccgggtg tcgatcgaac tcgtgcgcga cggcaagccg
    16681 cgcaccgtga ccgccaccct caaccagttg agcgaggcca ccgccggtcc ggttgtcggc
    16741 gacgacggcg cgccgcgtcc agccgccgcc gcgcaggcga atgcgctcgg tatcgtcggc
    16801 gaggacctcg acccgcagca gcgcagccga ctggggctgc aggccggcga gggcgtgctg
    16861 gtcgcgcggg tggaaggcgt ggcggcgcgc gaggccggcc tgcgcccggg cgacgtgatc
    16921 ctcgcggtgg gccgcaacga cgtgtccagc gccgccaggc tcaatgccca gttgcgcgcg
    16981 gcaggggcgg gcaagccggt gatgctgctg gtgcggcgtg gcggcggtac ccagtacctg
    17041 acgatcaacc cgaccgggga ctgatggcgg tggcaccccc gttcgtgcct ctcggggcat
    17101 gaacggggct gtgcgataat gtcgtgctaa ccccagacag gctgcgatcc gctgccgtcg
    17161 cagccccaca gccgagtctc catgcagcac gatgccatgc ggaatatccg caacttctcg
    17221 atcatcgccc acatcgacca cggcaagtcg accctggccg atcgcatcat ccagctctgc
    17281 ggcggcctgc aggaccgcga gatggaagcg caggtgctcg attccaaccc gatcgagcgc
    17341 gagcgcggca tcaccatcaa ggcgcagtcg gtttcgctgc cgtacaccgc gctggatggc
    17401 accacctacc agttgaactt catcgacacc cccggccacg tcgacttcag ttacgaagtc
    17461 agccgctcgc tggccgcgtg cgagggcgcg ctgctggtgg tggacgcggc gcagggcgtc
    17521 gaggcgcaat ccgtcgccaa ttgctacacc gcggtggagc agggcctgga agtggtgccg
    17581 gtcctcaaca agatcgacct gcccaccgcc gacatcgaca aggtcaagga agagatcgag
    17641 gcggtgatcg gcatcgatgc cgaggacgcg gtggcgatca gcgcgaagac cgggctgaac
    17701 gtgcgcgacg tgctggaggc gatcgtcaag cgcatccccc caccgaagcc gcgcgacacc
    17761 gacaagctcc aggcgctgat catcgactcc tggttcgaca actacctggg cgtggtcagc
    17821 ctggtgcggg tcatgcaggg cgagatcaag ccgggcgaca agctgctggt gatgtcgacc
    17881 ggccgcaccc accaggtcga caaggtcggc gtgttcaccc cgaagcgcaa ggagctcgcc
    17941 aggctgggcg cgggcgaggt gggctgggtg cacgcctcga tcaaggacgt gcacggcgcg
    18001 ccggtcggcg acacgctgac gctggcgggc gaccccgccc cgggcccgct gccgggcttc
    18061 caggaaatgc agccgcgcgt gttcgccggg ctgttcccgg tcgatgcgga ggactacccg
    18121 gacctgcgcg aggcgctgga caagctgcgc ctcaacgatg ccgcgctgcg tttcgagccg
    18181 gagagttccg aggcgatggg cttcggcttc cgctgcggct tcctgggcat gctgcacatg
    18241 gagatcgtgc aggagcgtct ggagcgggag tacgacctca acctgatcag caccgcgccc
    18301 acggtggtct acgaagtcct caagaccgac ggcggcatcg tgccgatgga caatccggcc
    18361 aagctgccgc cgatcaacca ggtgcaggaa atacgcgagc cgatcatccg cgccaacatc
    18421 ctgaccccgc cggactacat cggcaacgtg atcaagctgt gcgaggaaaa gcgcggcgtg
    18481 cagatcggca tcacctacat ggccagccag gttcaggtga gctacgaact gccgatggcc
    18541 gaggtggtgc tggatttctt cgacaagctg aagtcggtga gccggggcta tgcctcgctc
    18601 gactaccact tcgtgcgctt cgaggcgggt ccgttcgtgc gcgtggacac gttgatcaac
    18661 ggcgacaagg tcgatgcctt gtcgatcatc gtgcatcgct cgcatgcgga ccgccgtggc
    18721 cgcgaactga ccgagaagat gaaggaactg atcccgcgac agatgttcga cgtggccatc
    18781 caggccgcga tcggcgcgca ggtgatcgcc cgcaccacgg tcaaggcgat gcgcaagaac
    18841 gtgctggcca agtgctacgg tggcgatgcc agccgcaaga agaagttgct ggagaagcag
    18901 aaggccggca agaagcgcat gaagcaggtc ggcagcgtgg aaatcccgca ggaagccttc
    18961 ctcgccgtgc tgcaggtcga caacaagtga gtgcgctgcc ccccacggag aacgccgcat
    19021 gatggtgtgg ttcgaaacga tcctggtcgc cttgaccgcc ctcaccggcc tggtctggtt
    19081 gctcgacaag ctgttcctgg ccaaacgccg cgccgccgct gcaggtccgc tggacgaggt
    19141 caaggaaccg gtggccgtgg actattccaa ggcgttcttc ccgatcctgt tgctggtgct
    19201 tgggctgcgc agcttcgtgg ccgaaccctt ccggatcccg tccagctcga tgatgccgac
    19261 cctgctgatc ggcgatttca tcctggtcaa caaattctcc tacgggctgc gctggccgat
    19321 caccaacaag aagttcctgg ccaacggcga gcccgtccgg ggcgacgttg tcgtgttccg
    19381 gccgcctcac cacccgcgcg aagactggat caagcgggtc atcggcctgc ccggcgacac
    19441 gatcggctac cacgacaaca agctgagcgt gaatggccgc gaagtccctt accgcgtgga
    19501 aggcgcctac cagggcgaag gacagggcgc cgagatgacc ggtgcgtcca ggctcaccga
    19561 gggcctgccc gggcggccgc attttgtcct gcgccgcgac gacacgccgt tgccctacca
    19621 aggggaaggt gactggacgg tgccggaggg gcattatttc gtcatgggcg acaatcgcaa
    19681 caacagcgag gacagccggt tctgggagac ccacttcctg ccggaggcca acctgcgtgg
    19741 caaggcgatg atcgtatgga tgaatttcga ccgccaggcc gaccactggg tggacacctc
    19801 ccggatcggc aagcggattc cttgatgcaa tgaccgtcgg aaaggggaag cacgcatgaa
    19861 gcgcatgcaa cgcgggatga ccatgatcgg gttcctcatc accctctcgg tggtgatcct
    19921 cttcgtcttt tgcgggatga aggtcgtgcc gatgtacatc gagttctatt cggtgaagaa
    19981 ggcgcttgcc agcatcgcca acgagcagga caccagtggc gccaccaagg acaagatccg
    20041 cgcgatgttc gcccggcatc tgacgatcga ctacgtgaag gtcatcaagc cggaaatgct
    20101 gcagatccag accaccgacg acggctacaa catgatcgtc gagtacgagc gccgggagga
    20161 actgatcgcc aacctcgacg tggtcgccaa gttccgtgca gagcaggccc tggtgcgtgg
    20221 cgcagcggcg caatgacctt gcgccgcggg cattcgccgg acacgccttc gcggatccgg
    20281 cgctgctcga ccaggcgctg acccatcgca gcgccggcag tccgcacaac gaacgcctcg
    20341 aattcctcgg cgatgcactg gtcaaccagt tcatcgccga ggcgctttac ctgcactggc
    20401 cgagggctga cgaaggcgcg ctgacccgcg gccgcgccga gttggtgcgc gagtcggcac
    20461 tggcagggat cgcccgtacg ctggatcttg gcgtgcacct ggtactcggg ccgggcgaga
    20521 tgaagaccgg cggtcatcgc cgcgattcga tcctcgccga tgcgttggag gcgctggtcg
    20581 cggcgatcta cctcgatgca ggctttgatg cctgccgcgc agtcgtcctg ccctggttcg
    20641 atccgctggt cgcggcgctg ccgccggcga acaaggtcgg caaggacgcc aagacccggc
    20701 tgcaggaatg gctgcaggcg cgccagcgcg ggctgcccgt ttacacgctg gtcgaggaac
    20761 aaggcgacga ccacgcccgg gtgttccggg tcagttgccg cgtggccgaa ccggccatca
    20821 ccaccgaagg cgagggcagt tcccgccgcg ccgcggaaca acaggcggcg gaagccgcca
    20881 tgcaggtcat cgacccatga gcaaaccctc ccaacgcagt ggcgcgatcg cggtcatcgg
    20941 ccgtccgaac gtcggcaagt cgaccctggt caacgccctg gtcggtgcca agatcagcat
    21001 cacctccaac cggccgcaga ccacccgtca tcggctgctc ggtatcgcca ccttcccggg
    21061 cgggcaactg cagttggtgg atacccccgg catccatggc gagcagggca agcgctccgg
    21121 caaggcgatg aaccggatga tgaatcgcgc cgcgcgcggc gcgctggaag gcgtggatgc
    21181 ggcggtgctg gtggtcgagg ccggccgctg ggatgcggaa gatggcctgg ccttcgatgc
    21241 cctgcgcgaa ggcgggttgc cggtggtact ggtggtcaac caggtcgaca agatcaagga
    21301 caagggcacg ctgctgccgt tcatcgcaaa ggtcagcgag ggccgcgagt tcgccgccgt
    21361 gcatccgctg tccgcgctca agcgcaaggg cctggaggcg ctggtcgaca ccctgctggg
    21421 cttggtgccg gaagcggcgc cgatgttcgg cgaggacgag attaccgaca agagccagcg
    21481 cttcctggcc ggcgagctgg tgcgcgagca actgatgcgc cagctcggcg aggaactgcc
    21541 ctacgcgacg accgtcgagg tggagaaatt cgaggaagac acgaccggcc ggcagttggt
    21601 ctatcggatc gacgcggtgg tctgggtcga acgcgagggc cagaaggcga tcgtgatcgg
    21661 caagggcggc gagcgcctgc gcgagatcgg cacccgcgcg cgcgggcaga tggagcgcct
    21721 gttcgattcg aaggtgttcc tgcagacctg ggtgcgcgtc cgcgaaggct ggtccgacga
    21781 cgaagcggcg ctgcgctcgc tcggttacca cgactgatgt tcgctccctc ccgtgcgaag
    21841 caggggaggg cgggggacgg gttgggcttg tcgatgaggg taaacacctc cgtccagcag
    21901 cccggccctt cgacacggtc ttcgggcgtt catcggggcg cgaaccagtg gttcgcaagc
    21961 gtcgctcttc actccccttc gctacgcgca aagggagtgg cttgatccat gcgcttcgtc
    22021 ggtgaacctg ccttcgtcct ccacgcccgc gccnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22141 nnnnnnnnnn nnnggcttga tccatgcgct tcgtcggtga acctgccttc gtcctccacg
    22201 cccgcgcctg gcgggaaacc agcctgctgg tcgaagtcct gagcgccgaa cacggccgca
    22261 tcggcctggt cgcccgcggc gtgcaggggc cgaaaaagca tgtgttgcgg gcggccctgc
    22321 agccgctgca atcgatccgt ttcgatggcg tgcaacgcgg tgaactggcg caactgacct
    22381 cggccgaggc ggtcgatgcc gcgccgctgt tgcacggcga tgccacgctt ggcggcttct
    22441 acctcaacga gctgtgcatg cggctggcgc cgcgccaggc gccccaaccg gagttgcatg
    22501 ccgcgtacgc tcgggtgcgt gaacgcctgc gcgcgggcga gccattggcg tggatcctgc
    22561 gccgcttcga gcgcgacctg ctggacgcgc tcggcagcgg tttcgattac gccagtgatg
    22621 gcgaaggggc accgatcgac cccgccgcac gctaccgcct ggaggccgag catggtgcac
    22681 gccgcgtgtt gggcgaacgc gcgggcgaac gtcgtgaaag cgccaccggg cgcgccttgc
    22741 tggcgctggg cggggacagc ctgccgaacg gcgaggacct cgccagcctg cgcctgccgt
    22801 tgcgcggggt gttgcttcac caccttggcg ggcgtggcct gaagtcgtgg gagatgctgg
    22861 gcgagctggg acggctggcc gcacccaagc cctagccctc gatcaatgca tcggcggctg
    22921 cggtgaatgc ctgcattgcg gcgggatcgg tcggatcgtc catcagcctg cgcaccgcat
    22981 cgcgcaaacg atccgcgccc acgaacccgc aactcgcggc cagccggtgc aggtccgcct
    23041 gcatcgtcgc ggcgtcgccc atggccgcgg cgatgcgtgc gcgttgtgcc ggcatttcgc
    23101 gccggaacag ctggcgcagc acgtcgacat gggccgcatc gccgagggcc gcctgtgccc
    23161 cggcatcgtt ccagtcgggc aagtccgcgt cgccgacccg atgcaccggc atcggcaagt
    23221 gccggcgcag tgcggcgtgc aggcgggtca ccgagatcgg cttgcacaac acctcgagga
    23281 aaccggcggc gagcaagcgt tcgcgggtcg ctgcgttgtc gtcggcggtg tgcgccagcg
    23341 ctgccgctgc acccgtcgtg gcttcgagca ggatttcacc cgggccgtcc ggcaggttgg
    23401 catcgaccag ccacaggtcg aacgccatcg ccgcgatcgc ccgccgcgcc tcggccaggc
    23461 tgccggcgag ctgcaccacc gccggcaggc cactcgctgc atcgcacagg aacgccgcac
    23521 tgaccgggtc gtcctcgacc agcaacagtc gcggcaaggg gctgctggtg gcgggattca
    23581 tcgcgaagcg acctcggctt tgtatgctgc gggcatgtgg cgttcgatgt taacggcttg
    23641 ttccctgctg gcgatgctgg ctgcagcgcc cgtgcatgcg cgcgcggcgc aggtgcgcat
    23701 cgccagggtg gccacgccgg tcgccacgct ggaacaggtg cgggtgcgac tggactggcc
    23761 ggaaggtgcg gcgaccggcg agttgcaagt gtgggccggc aaggtggacg ccgcggacct
    23821 cggataccgc tatcgcgact tgcattggcg ctgcccgttg cggcgcgcgg cccatgatgg
    23881 ctggcagtgc gacggggcgt tgcgcgccgg catggccgcg ccattgcggc ttgccgtgca
    23941 gttcgacgca gcgaccaccc aggcatcgct ggcgaaaggc gatgcgcgtt tcaccctgga
    24001 tcgcagtgcg gccacgccgg acctgacgtc gctcgacctt acccgggtac cgatggcctg
    24061 ggcgcaggca ctgctggcgc aggcctggcc ggcagcacgc ttgacgggcg gccagctgga
    24121 cgcgtcgctg gcgatcgaag cgcccgccaa ccgcccactg cggatcagcg ggcagctgcg
    24181 cccgcgcggc gtcggcatgg aaaccgcgga tgcgacgatc gccggggaga acctgggcgg
    24241 cgacctgcgc atcgactacc gcaccacgcc tgcgcggtcg tggttggcgc tgctcggcga
    24301 actgcgcggt ggcgagttcc ttgccggcag tacgtatgtc gccttgccgg cgacggcggt
    24361 cggattgcgc attgatgcga tgaagcaggc gggttccggc tggcaggtac cgcggatcga
    24421 atggcgcgac ggcgaggcct tgcaagcgaa cgggacactc gcattcgaca aggatggcgc
    24481 gttgtcatcg atggcggtcg atgccggtag cggcgacatg tcgccgctgc gccaacgcta
    24541 tctctccggc tggatggggt tgtttggcct cggcgatgtc gacctgcacg gggcgatgga
    24601 cctgcacgtg gaggcgcagg gtggggggct ttcacggatc gacgcgatcc tgcacggggt
    24661 cgacctgcgc gacccggcgc ggcgcttcgt gttcgacgga ctcgacggcg atttgcggta
    24721 ctcggcatcg gcgccggtgc gcagcgaact gcgctggagc ggcgggcagc tgtacggcct
    24781 cgcgttcggc gcggcgcgcc tgccgatggc cagcgccgac ggccagttgc gcttcggcga
    24841 agacgtgcac gtgccgctca tgggcggcca gctgaccctg cgcgacgtgg tgatccagcc
    24901 gccgcgggat gcggcgggca tgcagatccg cttcgggctg gggctggacg acatcgactt
    24961 cggcaaggtc tcgcaggcgc tgggcttgcc ggccttccag ggacgcctga gcggcaggat
    25021 cccgcaggcg cgctacgccg acgaacgcat cgaattcgac ggtggcctgt cgctgcggtt
    25081 gttcgacggc cgggtggcgt tttcggcgct ctcgctggag cgcccgttcg gcagcgcgcc
    25141 cagcctgagc gcggacatcg cgttcgatgg cctcgacctg ttgcgactga cggaagtgct
    25201 cggctttggc agcatcactg gccgcctgga cgggcgcgtc gagggcctgc gcctggtcga
    25261 ctggacgccg gtggcgttcg acgcgcgctt catcaccgac gaagcgccgg gggtgaagca
    25321 gcgcatcagc cagcgcgcgg tgcagaacat cagcagtgtc ggcgacgcgt ccttcgtcac
    25381 cagcctgcaa ggccagctga tcggattgtt cgacgatttc ggctatcgca ggatcggcat
    25441 cggctgccgc ctggccaacg aggtctgcgc gatgtcggga ctggattcac atcgcagtca
    25501 gggcgggaac gcctttacta tcgttgaagg tgccggcctg ccgcggctcg acgtggtcgg
    25561 tttcaatcgc aacgtggatt ggcccaccct ggtcgagcgg ctggtcgcgg tcggcaaggg
    25621 cgaagtggcg ccggtcgtgg aatgacggat cgtcgaatga tgcacacagg agcaaggtca
    25681 tgaagcagtg gttgggcatg ccggtcgcgg gcgcgttgtt gttgagcgcc tgcgtgacga
    25741 tcaacgtgta tttccccgcg gcggaagcga aggaagcggc gcgcgagttc gtcgaaaagg
    25801 tcatcaacga agcggacaag gtcgagatga aggatccgtc cgcgcccgcc ggcggcatgg
    25861 catcgctggg cacgcggatc cgctccgacc tgtccggttt cgatccctgg gtgctggtcg
    25921 gcatcggcag cgcgcaggcg caatcggcgc cggacatcag cctgaagacg ccggcgatcc
    25981 aggcgatcca gtcgcggatg gaagcgcgct tcaatgccac gctgcggggt ggcttcgaca
    26041 gcggcgcgct ggggttcacc gccgacggtc tggtgaccgt gcgcgatgcc ggcaagctgg
    26101 aactgaagga ccgggtcgcg atgaatgccg cggtcgccga cgacaaccgc gaccgcaagg
    26161 cggtctatcg cgaagtcgcc gtggccaatg gccatccgga gtgggaaacc cagatccgcg
    26221 acgtgttcgc ccgccagtgg atcgccagcg cgcgtccggg ctggtggtac cagtccggtg
    26281 gaagctggaa gcagaaatga cttcgatgac ggcccgcttc ggcgggccgt tcgctttcac
    26341 ggacccgtcc tgcacatatc tgccaaaata gcgggccacc ctgctgccag ccgtcacgtg
    26401 gcccgactcg atcattcccc caagtcgctc gacatcgtca acctgaccca tgacggtcgc
    26461 ggcgtggccc gttggcccga cggcccgcat gccggcaaga ccgtgttcgt gactggcgca
    26521 ttgccgggcg aaaccgtgct ggtccagcag accgcgcggt cgcgcaattt cgacgaagcg
    26581 aagaccctgg aggtcgtgca ggcatcgccg gaccgggtca cgccgcgctg cccgcatttc
    26641 ggcacctgcg gcggctgcgt gttgcagcac ctcgcggaag acaagcagat cgaagccaag
    26701 cagggcgtgt tgctggacaa cctgcaacgc atcggccacg tgacaccgca gtcggtattg
    26761 ccgccgttgc gcggcgacag ctggggctac cggcgcaagg ggcggttctc ggtgcgccgc
    26821 gtcgagaaga aggacaagac cctggtcggc ttccgtgagc aggatccacg cttcgtcgcc
    26881 gacatcggcg aatgccacgt ggtcgtgccg caactcggct tca
//
LOCUS       DAOOCX010000020        26585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1503_length_26585_cov_7.960271, whole genome shotgun sequence.
ACCESSION   DAOOCX010000020 DAOOCX010000000
VERSION     DAOOCX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 26585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26585
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1503_length_26585_cov_7.960271"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1498
                     /locus_tag="PK399_03350"
     CDS             35..1498
                     /locus_tag="PK399_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW12189.1"
                     /translation="MAHQQTRWSQFGVLITVFFFWGFVAASNDILIPVFKKAFNLSQA
                     QSQLVSLAFYVAYTVGSLIYFLTSKLIGADVLNRIGYKNGICLGLLISAMGSLLFYPA
                     ANSGSYGLMLSGLFIVGLGFSLQQIAANPLAIVMGNPATGAQRLTMAGGVNNFGTTIG
                     PLLVSFAIFGSAAAGGTEASIESVKIPYLVLGAAFVLVAVFLKFSSVPNQVDLDALAA
                     DETSDASKLAHKSSAFGYPQLVLGMIAIFVYVGVEVSTISNLPAYLSLPVSEGGLGIE
                     TALIAPFVSLYWASLMIGRWAGAAGAFDVAKHTKWMLTILLPYLAFGVFLAVNAFANH
                     EISHFFMYGFVIVLMIAATFASRGNPARMLLYFAICGIAALVIGMLTTGMTSVVAFIS
                     VGLFCSTMWPCIFTLAITGLGRNTNQGSSLLIMMIMGGGIVSWLQGVIADMHGIHYSF
                     VVGVLCFAYLAFYAVAAKHALQKQGIDLDALTAQGGH"
     gene            complement(1527..2597)
                     /locus_tag="PK399_03355"
     CDS             complement(1527..2597)
                     /locus_tag="PK399_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW12190.1"
                     /translation="MSTTRGAGEAASEAAGRIRVARVRIEDVAEQAGVSMKTVSRVLN
                     NEPNVSVRTRTLVEAVVEELRYRPLPSARVLAGRRSYLVAMLFDNPSSNYLMEIEMGV
                     LDACQAQHYNLMLAPLVYDANDIIDKVEALVMQAQLDGVILTPPITDDVALLRRLEEL
                     GIPYSSISAKEQNRHVGVVVDEHGAVCAMMAHLASLGHRRIAHIRGHAAHGASAWRLA
                     GYREGLRRAGLRYDARLVVDGEFSYQSGYAATNALLDLDDRPTAIFAANDDMAAGAIC
                     AICERGLSVPGDISVCGFDDTPIAHQIYPALTTVRQPTREMGRLAATELLKEVRELGS
                     GGIVDVAYALQLRRSTGPAARD"
     gene            2772..5333
                     /locus_tag="PK399_03360"
     CDS             2772..5333
                     /locus_tag="PK399_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPW12191.1"
                     /translation="MSRRPANTLSRPRLALLSALAAAVFIAHAAPAPQEAATGNAINP
                     GHWPQPTWPLATDAALEHRLEALLAGLTVEEKVGQIVQADIGSITPDDLRKYRLGSIL
                     AGGGSDPGGQYNASPQAWLALADAFWEASMDTSQGGKAIPVIWGIDAVHGQSNIIGAT
                     LFPHNIGLGATRDPALVREIGRITAAETRVTGMEWTFAPTVAVPQDDRWGRSYEGYSE
                     SPGVVAQLAPAMVEGLQGKAGTRVFLDERHVMTTVKHFLGDGGTTNGKDQGNTEVTEA
                     VLRDVHAAGYVPAIATGAASVMASYNSFHGEKLHGSKALLTDVLKTRMNFGGFIVGDW
                     NGHGQVPGCSNTDCARTFNAGLDMAMAPDSWRGLYDSTLVHVKAGTIPMARLDDAVRR
                     ILRVKLQMGLFEQPRPSQRALGGKFELLGAAEHRAVARRAVRESLVLLKNNGGVLPLD
                     PRRRILLTGDGADNLGKQTGGWTLTWQGSGTSREDFPNADSIWDGFRQQVERAGGHAE
                     LAVDGTYSTRPDVAVVVFGEEPYAEFQGDLPNLMFKAGRSGDLELIRKLKADGIPVVA
                     IFLSGRPLWMNREINAADAFVAAWLPGSEGAGIADVLLRDGRGKVQHDFHGKLSFSWP
                     RRPDQYTNNAGQPGYDPLFPLGHGLRYADRGTLAPLSEDPGIDPNDARSNLWFDRGVG
                     TTGLTLQLVGAGGVAMDVIHPAAATADGSLAMEAINTTIQEGARRFDFKGAGEVLLRA
                     NAPLDLVRETNGDVFVVATLRVDALPAGGKATFRVDCGDGCRGELPIGDTLAALPRGQ
                     WTRLGVQLRCLREAGADTGKLSVPFALRGSAGLRLSLSQVALGTAFDQRVECSLQ"
     gene            complement(5373..6839)
                     /locus_tag="PK399_03365"
     CDS             complement(5373..6839)
                     /locus_tag="PK399_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HPW12192.1"
                     /translation="MSANARVRRCAGWSLLLAAMLGGCARPSPPPGTDQVQVWTTTAD
                     HRLALAPSRVGFDAGDAVDGATTGAALIEIDASARHQQMVGFGVSITDASAWLIRQKM
                     SPAQREALLRELFGRDDDGIGFDFARLTIGASDFSRHHYSLDDPPEGQPDPALRHFSL
                     DVNRGDLIPVARQALAINPELWIMASPWSAPAWMKDSGHLITGRLSPDHYDAFARYLL
                     RYVEAMAGEGISIRALTVQNEPDFESKDYPGMRLNAPARARFIGDHLGPLLARQAPQV
                     QLFDWDHNWDKLEEPLAVLADPVAARYVDAVAWHCYAGQPVAQSQVRRAHPDVDVYMT
                     ECSGGDWEPVRSGGLTLQVERLIVETTRNWARGVLFWNLALDENSGPHAGGCGNCRGV
                     VTIDSKTGEVSRTDDYYALAHASRFVRRGAWRVASSEALAGVANVAFVNHDDSRVLVV
                     SNADPQPRAIRVREGGRSFRHLLPGGSVVTFRWHPAGR"
     gene            6987..9773
                     /locus_tag="PK399_03370"
     CDS             6987..9773
                     /locus_tag="PK399_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW12193.1"
                     /translation="MLYPYTRATRRATQTAAMRRQHAILPRASALALACSAVLAFSAQ
                     AQQVEPAKTPPQESAKNLDKVVVVGIRSSIATSVEAKNESTSIVEVVSAEDIGKLPDI
                     SIADSISRLPGLTMQRLDGRGQVIHIRGMSEQFAGTLLNGREQVSTGDSRGVEFDQYP
                     AELINSVTVYKTPDASLIGQGLSGTVNMQTVRPLDFKERKVVLGVQGDHNSFGELTAG
                     GKDTGYRVSASYIDQFANDTVGLAVGVARLNAPFQEKHYKSWWWANTTLWGAPQGAKP
                     ADAIALQGAEAWVKSRDTTRDGVMAVLEFKPNDAWHSVIDAYHSKFDQSEWMRGAMWT
                     NDPWWNNGAVTYTNATTTNYNGTPVVTGGTLSGIRPVVRNDNNLRDDSLSAFGWNTEY
                     KTGAFTVAADLSYSRAVREQSTLETYAGMTSGLNVDFRIPLSAGYAWYGLPDLSAPGS
                     VKLGDPQQWGHDGRMENSRQVDTMKAFRLSLNHALDSAFLSSYDVGYNFSRRSKDKHA
                     DVYFADLPGRVPVAVDPSLLQSPTDLGFAGMGSVLTYDPRALLSNYYNVYLSETNDDL
                     QKDNRIQEEIGTFYAKANIDTTLGERVNLRGNIGLQYVRTNQKSNGVGITPAGPAAVI
                     GVGTTYNDVLPSLNLVFDFNNGWNLRFGAAEEQMRPRINDMGAYANVGVSVGNGVAQW
                     SGRGGNPKLEPFRAKAVDISVEKYFGPASYIALAAFYKDLESYVYTSTIPWDFTGYEF
                     DTPIPPQSNIGTFSTPANGKGGYMRGVEFSTALGGELLHPALSGFGLLLNGSYTESSI
                     DPDGPGGSATDTIPGLSKIVANATVFYEKNGFSARVSERFRDPYRGEYSYIFGQRSYR
                     YTRNERTIDMQLGYDFPEASRMRGVSLLLQVNNLNNEPFRTQVSDSNGLGLMFPEEYT
                     EYGRQYLLGMRYSF"
     gene            9859..10578
                     /locus_tag="PK399_03375"
     CDS             9859..10578
                     /locus_tag="PK399_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HPW12194.1"
                     /translation="MIDLYYWPTPNGHKITLFLEEAGLDYTIKPVDIGKGAQFEADYL
                     RISPNNKMPAIVDHAPVDGGAPISVFESGAILLYLANKTGRFLGIPDGADAGTALRQR
                     ILVNEWLFWQMAGLGPMTGQYGHFHVYAPEDIAYAKDRYRKEAERLLGVLDKQLEGRT
                     FIAGDDYSIADMASYPWIGPYDKAPLDLAPFANVQRWRAEIRARPATKRAYALTRQVS
                     PNAGKPLSDEEKKVLFGQGAR"
     gene            complement(10666..12996)
                     /locus_tag="PK399_03380"
     CDS             complement(10666..12996)
                     /locus_tag="PK399_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPW12195.1"
                     /translation="MGRIDAKQAWLGAMLVTGVGMGSAMAQAPQVTVADYERAASMLG
                     DRTSPLVDHATSGIGWLDDGTLVYRERSNGVERVLRLDPATGKTTPAFNAQALADAMN
                     LAAGGKGRPVDAGKLPPLRVVRNADGSLRLAGMQGEFRCDAAGCGALAKPASGEEPGA
                     ASPDGTREAFIRDWNLWLRDRASGKETQLTFDGKPDYGYATDNAGWKHTDEAILVWSP
                     DGSRIATFQQDQRKTSTMTLVATSAGAPKAEQWKYPFVGDKDVTMIERVIIDLSAAKP
                     KTIRLQLPPDQHRSTCSDDIACSEGWEDVQWAKDGKTLAFVSTDRGHKSATLRVADAA
                     TGKVRDVFSETVATQYQSAPALDSVNWRYLPESNEFLWFSQKSDWGHLYLHDLASGKE
                     KRQLTSGNWNVAKVAHIDAASRTLWFEGVGREAGRDPYFAHFYKVGMDGGDVQLLTPE
                     NAAHTITPSKDARYFVDVYSTTRNPPVAVVRDAAGKQVAELARADITRLKAAGWVAPE
                     DIVVKARDGKTDLYGLMFKPSTFDAAKKYPIVTYIYPGPQVGSVGRRGFSPSRSDHQA
                     LAELGFIVVAIDGMGTPLRSKSFQDAWYGNMADNTLPDQVAALKELAAKYPWIDATRA
                     GMWGHSGGGNATATAMFRYPDVYKVGIAESGNHDNLAYEDDWGERYHGLLETKADGTS
                     NYTGQDNAAYAKNLKGKLFLLHGLMDDNVPPQSTLLVVDALVKANKDFDLLLLPHARH
                     GYGRDSYYVMRRRWDYFVEHLLGATPPKEFELKPKM"
     gene            13119..13946
                     /locus_tag="PK399_03385"
     CDS             13119..13946
                     /locus_tag="PK399_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW12196.1"
                     /translation="MNDQPLPLTPERIAVVVPALNEALRIRDVVQGALRHCPNVIVVD
                     DGSDDGTADAVADLPVVLLRHAARMGKGAGLRTGFAAALQRGFAAVITMDGDGQHSAD
                     DIPRLLAAANRRRGWIVIGARLRKRANQPLYRRLANEFGDWGIAWGTGYQVADSQSGQ
                     RLYPADVCALACAGIPGEEFVFEAQVLMSAAQELGTRCVSVPIEARYNVVHAAPDFRP
                     SHFRPLRDLWRITSHIVLQCLRRGGLWQMHRRVRANPPVIDDPAGEFATDLAAAQPR"
     gene            13986..14987
                     /locus_tag="PK399_03390"
     CDS             13986..14987
                     /locus_tag="PK399_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine-dependent deoxygenase"
                     /protein_id="HPW12197.1"
                     /translation="MPSLPEPLPHAAATLTVEYRHDPLDAMLADPRLLAVFGFGDQAP
                     AMHGDPRYLRVDLPVRGDAPFECWRVDNEVHHGRRDGIAWSEDGHLQFGALDVADAGD
                     GDIERAAEQAYARLQAWLAAGDYPHPLRIWNYLDAITAGEGDDERYRRFCVGRARGIG
                     RALGPAELPAATAIGHPRPSGKLQVYWLAAKTPGTPLENPRQVQAWRYPRQYGPQAPG
                     FARAMLPASIAMPLLVSGTAAVVGHASQHGDSLQAQFEEVLANLGSLVERARALRPGV
                     PDRPGNGTRLKIYVRDADALPTVDALLRQRLPGVPALLLHGHVCRRELAVEIDGVHA"
     gene            15035..15283
                     /locus_tag="PK399_03395"
     CDS             15035..15283
                     /locus_tag="PK399_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12198.1"
                     /translation="MGLISILWGIVAMGWMVLALIPLVGITNWLLIPFAAIGAILAAI
                     GIAVTSAGKRGRAKAGLILNCVVIVVATWRLGLGGGLI"
     gene            15459..15773
                     /locus_tag="PK399_03400"
     CDS             15459..15773
                     /locus_tag="PK399_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12199.1"
                     /translation="MSSNNKITGALAAFAVAALMAGPAVAGTPLNVVQKKALADAKAL
                     QAPSPTAGQTLTGADGSPAAVVPDELHNYMHAQRDASGKVQVLETDGTTAPAKAVEVT
                     NE"
     gene            15766..17382
                     /locus_tag="PK399_03405"
     CDS             15766..17382
                     /locus_tag="PK399_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPW12200.1"
                     /translation="MNKLRNALAVAVLAALAAASSAQAADLIPVNLDGANEGYNDNTP
                     ATPVGGNYGTTKGEQRRIVAQYAAAMWGSVLKSDQPIYIAAQFNPLGANVLGSAGAVT
                     VHANFGNAPVANVWYPAALADAISGIDLAPTSYDISSQFSSDFSFYYGLDGKTPAGQI
                     NFLDVVMHEFGHGLGFQNFETEATGAFLSGRADIYSVYTYDNTTGKFWTAMTQAERVT
                     SALNYGNVVFTGANATAGAKLLLDDRKDFRVTAPAPVAGNYAIGSVTFGPLISTTNFN
                     GQVVLGTDSVGTPGDGCEAITSNVAGKIALIDRGVCTIAIKTKNAQDAGAIGVIIANN
                     AAANPPPNFSGVDPTITIPTIVVTQAAGTSFKANLPMTVTYVVDATKWQGADDLGRPR
                     LFMPNPVQSGSSGSHYDNNAMPNLLMEPAINDSLRSYYNLDITPHLLKDIGWQLNTAS
                     AKIGSPNVPDCDTGLSGTPSGGLFVAGTIQASNELCLISAQTRGAYYSCMDAARDRMV
                     AAGVLTSLQGNKVMMCARRVQDHQVFPIAF"
     gene            17459..18466
                     /gene="hemB"
                     /locus_tag="PK399_03410"
     CDS             17459..18466
                     /gene="hemB"
                     /locus_tag="PK399_03410"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPW12201.1"
                     /translation="MTSPAAQRFPVRPRRMRHDAFSRRLMREHVLTANDLIYPVFVHE
                     ETGRAPVGSMPGVERLSIDELLRVGEQALELGVPVLDLFGVPDPSAKTADGRIAWDED
                     GIIQRAIRALKSRFPELGVMSDQALDPYTTHGQDGLVDDSGYILNDETIEALVKQSLS
                     HAAAGADILSPSDMMDGRIGVIRDALEANGFIHTRIMAYSAKYASAFYGPFRSAVGSA
                     ANLGKGNKYTYQMDPANSDEALHEVGLDLAEGADMVMVKPGLPYLDVLYRVKQHFKRP
                     TYAYQVSGEYAMIKAAAANGWIDEKMVAMEALTAFKRAGADGILTYFALDAARWLREG
                     H"
     gene            18495..19337
                     /gene="aroE"
                     /locus_tag="PK399_03415"
     CDS             18495..19337
                     /gene="aroE"
                     /locus_tag="PK399_03415"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011257342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPW12202.1"
                     /translation="MTRHYAVFGTPIAHSLSPRIHAAFGKQTGIDLEYVAIEAGPEDF
                     TRKLDAFAARGGLGANITLPLKRRAAELAATLGDRARRTGVANTLVRRGDGWHADNTD
                     GSGLVGDLTLRQGLDLRGRRTLLLGSGGGARGIAPALLDAGIDALYIVNRTAQNADAL
                     ADLLGDPKRVHARYLDDLPALGEFDLIVNATSATRGGALPHLPMSMVGRRTAAVDLSY
                     GEAAIPFLAWARANRCHDAIDGLGMLVEQAAESFEVWHGVRPDTDRVYADLRAHTDSL
                     VTAD"
     gene            complement(19354..20664)
                     /locus_tag="PK399_03420"
     CDS             complement(19354..20664)
                     /locus_tag="PK399_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPW12203.1"
                     /translation="MPLVLCCVGWALLGLATSFGVLPAAAWWAAGALLLLVALVDAVR
                     IVRQPLPEVARSLPDALPLGVERNVTLVLDAGARAQRVDVHDLHPTGWIATGLPRRLQ
                     LAADASTRFDYALRPTERGDARFDGVHLRLHSPWRLWTRARVVGAQRTVRVYPDFAPL
                     ARLALLGAETASRAVGAHVKRRRGEGTDFHQMRDYRVGDSLRQVDWKATARMRRLISR
                     EYQDERNQHLLMVIDTGRRMLAREPALAKAGGELSHFDHVLNAALVVSYLALRQGDAV
                     GMLASGGERRWVAPQRGMGAIDVFLRATYDLQPRPVATDYLAAATELSQRHRRRSLVM
                     LVTNLRDEDSDDLLGAVRMLQQRHHVVVASLREEALDAVLDEDVRDLRGALRAGAAAR
                     YIAQRTAVHDALRSHRVGVLDVTCEQLPGALVERYLAIKRDGLL"
     gene            complement(20673..21665)
                     /locus_tag="PK399_03425"
     CDS             complement(20673..21665)
                     /locus_tag="PK399_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPW12204.1"
                     /translation="MSIDIAPTIAPLAGEALGERASAIRAEVGKAFIGQDDTLDQVMV
                     ALLAGGHVLLEGVPGLGKTLLVRALSQALGCAHARVQFTPDLMPSDISGHAVFDPKLE
                     RFEVRRGPVFTNLLLADEINRAPAKTQSALLEAMQEGQVTIEGTSFPLPPPFMCLATQ
                     NPLELEGTYPLPEAQLDRFLLKVLIGYPSLADETRMVAAVSEGRMASDFDLGQVQRVA
                     SQDEVLAMQLGTAQVRVDPAVLDYAVRIAAATRKWPGIALGAGPRGSIGLVRAARAQA
                     VLGGRDFVTPDDLREIAKPVLRHRVSLAPELQIEGQSADDVLHALLAKVDAPRQ"
     gene            complement(21662..22783)
                     /locus_tag="PK399_03430"
     CDS             complement(21662..22783)
                     /locus_tag="PK399_03430"
                     /inference="COORDINATES: protein motif:HMM:NF025617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HPW12205.1"
                     /translation="MSRARAALPWLLALAVIAAGVAWFLATHHRVEKIVPMPPRGEAS
                     YNPLYALKLALRAEGQRVESRQRLQLDEMALAAGDTLLVYSDPRLLGDDQVEALFEFA
                     EQGGHLVLRLPSWQPDESFAGALADWLPIEPELVDPHCMDLFVPGQEDHVEFCGAPRF
                     ELVDGADVSAAWRTRDGHHVFARFPYGDGSVDLLGDMEMIDNQSLDEGPHLAFARQLL
                     APNWGKGRFHLVYAADMPPLWRWLLAHAWAFLLPLLLGLLAWLWMRMQRFGPLLPSPM
                     PPRRSLLEHVEASGEHLLRYGKLGVLHRALRDAVLARLRRRDPLAAAQDSETQAALVA
                     SRVAMTAAEVRATLDTRPPVNTTEFRHRIARLIDLRKRL"
     gene            complement(22780..24351)
                     /locus_tag="PK399_03435"
     CDS             complement(22780..24351)
                     /locus_tag="PK399_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HPW12206.1"
                     /translation="MRLDQPRVELRPRSPWEAMELGTALVRTHARAIWLPWLLVTLPV
                     FALLTVIGWFTGLLPWVAVAMWWLKPVFDRIPLFVLSRALFSEAPGVRETLAAQRTWG
                     WRAMPAYLTWRRLGPTRALYLPVDLLEGGANPGPRRRVIGGSVRGTAFLSTLVCMGFE
                     AALLFGAYALVMVFVPVEFMSESARAMWALLTVEPPPWVQVAGNAVAWLATSLVEPFY
                     VGAGFGLYLDRRTQIEGWDIEIAFRRMRHRLRALLPLLLVACALGLSMPRPAQAQVPP
                     APTPTDDAAARPAPATPAVKAPKSSQPPTLPMVFPQLQDPARFDKAVKRAYADPLLSP
                     KRKETRWERRKPPKPGEPPKPGEFPAIIGKIIAAIGEYGLWLLAAFAACLLLVTHRQW
                     WPWLRGIAPPPRQAETPVASEPHLATDALPDDIAGTARQLWQDGRKRRALALLYRASV
                     AGMVARTGTVLVPGATEAQCLRAARGLHDQADRDAFGRMVRVWQYAAYAERLPDDGEF
                     EGLLAGLGGRFGWAA"
     gene            complement(24341..25330)
                     /locus_tag="PK399_03440"
     CDS             complement(24341..25330)
                     /locus_tag="PK399_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HPW12207.1"
                     /translation="MRQEHFIVRHEAEWQAFEQWLDARGARPRRARADRSWRGLPDED
                     VPAAYRRVCQQLGLARRRGYSPQLVARLQELMQRGHAVMYRPPLPRWQRAFEFLLADM
                     PRLVRAEAGVMWAALALFAIPLVASYVGVQVKPELIHTLMGAQQVAEVESMYDPAKST
                     LGRAAESDLMMFGYYIFNNISIGLRTFASGLLAGLGPALALAFNGVFIGGVAGHLQAT
                     GHGEPFWRFVAGHSAFELSAIVIAGGAGLRLGLDLIAPGQRRRVDALVEGGRRGARLC
                     LGVFAMLVAAAFIEAFWSSIGWIPAWGKYSVGGVLWALVLAWLWFGGRDRHAP"
     gene            complement(25338..26018)
                     /locus_tag="PK399_03445"
     CDS             complement(25338..26018)
                     /locus_tag="PK399_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPW12208.1"
                     /translation="MLDTVREVHTPEGVALRLPAAGPVPRALAWLIDFGIRMGLLLAA
                     SMVLGALDKAGVGVYLLVLFALFWLYPILCEGLFDGQTPGKRALDLRVVSSDGAPVGW
                     LATCVRNLMRTVDMLPFGYACGLVSGLFDPWGRRLGDLVAGTMVVHVPKTRPLSVMVA
                     DGVMAPVLPLRPEEQAAIVAFAERAPRLTPERQQELADIAAPATGAQGGEGVRRLAGI
                     AHWLLGKR"
     gene            complement(26018..>26585)
                     /locus_tag="PK399_03450"
     CDS             complement(26018..>26585)
                     /locus_tag="PK399_03450"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPW12209.1"
                     /translation="PRSEGQHMDPYNSADGQAPSAPPANPYAAPDARVADIVHGQAQM
                     KSSRGVRLGAVLLDSLPVVVLAILAAIALPATQNANGQGISPIGMVILVAMVVGMIAF
                     AIYQLVQLHRSGQTFGKKVLGIRIVRNDGTRASLGRILGLRYFVPGMLGGIPLVGPLF
                     TLLDSLFIFGDAKRCIHDLIADTIVVDA"
BASE COUNT         4336 a         9460 c         8849 g         3940 t
ORIGIN      
        1 gccgcgacga catccacaaa cacggcggaa tcacatggcc caccagcaaa cgcgttggtc
       61 gcagttcggc gtcctgatca cggtgttctt cttctggggc tttgtcgccg ccagcaacga
      121 catcctgatc ccggtgttca agaaggcgtt taacctgtcg caggcgcaga gccaactggt
      181 gtcgctggcg ttctacgtgg cctacaccgt gggctcgctg atctacttcc tcacctccaa
      241 gctgatcggc gccgacgtgc tgaaccgcat cggctacaag aacggcatct gcctgggcct
      301 gttgatctcg gcgatgggct cgctgctgtt ctacccggcg gccaacagcg gctcctacgg
      361 cctgatgctg tcgggcctgt tcatcgtcgg cctgggcttc tcactgcagc agatcgcggc
      421 caacccgctg gcgatcgtga tgggcaaccc ggccaccggc gcgcagcgcc tgaccatggc
      481 tggcggcgtc aacaacttcg gcaccaccat cggcccgctg ctggtcagct tcgcgatctt
      541 cggcagcgcc gccgccggcg gcaccgaagc cagcatcgaa agcgtcaaga ttccctacct
      601 cgtgctcggc gcggcgttcg tgctggtggc ggtgttcctg aagttttcct ccgtgcccaa
      661 ccaggtcgac ctcgacgccc tggcggccga tgaaacctcg gatgccagca agctggccca
      721 caagtcgtct gcgttcggct acccgcaact ggtgctgggc atgatcgcga tcttcgtcta
      781 cgtcggcgtg gaggtctcga ccatcagcaa cctgccggcg tacctctcgc tgccggtttc
      841 cgaaggcggg ctgggcatcg aaaccgcgct gatcgcgccg ttcgtgtcgc tgtactgggc
      901 cagcctgatg atcggccgct gggccggcgc ggcgggcgca ttcgacgtcg ccaagcacac
      961 caagtggatg ctgacgatcc tgctgcccta cctcgcgttc ggggtgttcc tggcggtcaa
     1021 cgcgttcgcc aaccacgaga tctcgcactt cttcatgtat ggcttcgtca tcgtgctgat
     1081 gatcgcggcg accttcgcca gccgcggcaa cccggcgcgc atgctgctgt acttcgcgat
     1141 ctgcggcatc gccgcgctgg tgatcggcat gttgaccacc ggcatgacca gcgtggtcgc
     1201 cttcatcagc gtcggcctgt tctgcagcac gatgtggccg tgcatcttca cgttggcgat
     1261 caccggcctg ggcaggaaca ccaaccaggg cagcagccta ctgatcatga tgatcatggg
     1321 cggcggcatc gtcagctggc tgcagggcgt gattgccgac atgcacggca tccactacag
     1381 cttcgtggtc ggcgtgctgt gcttcgccta cctggcgttc tatgcggtgg ccgcaaagca
     1441 cgcgttgcag aagcagggca tcgacctgga cgcactcacc gcacagggcg gtcactgacc
     1501 acggggccgg cgcgccgtcc gcgcactcaa tccctggcgg cgggtccggt cgaccgtcgc
     1561 aactgcagcg cgtaggccac gtcgacgatg ccgccgctgc ccagttcgcg cacttccttg
     1621 agcagctcgg tggcggccag gcggcccatc tcgcgggtcg gctggcgcac cgtggtcagc
     1681 gccggataga tctggtgcgc gatcggcgtg tcgtcgaaac cgcagaccga aatatcacct
     1741 ggcaccgaca gcccgcgctc gcagatcgcg cagatcgcac cggcggccat gtcgtcgttg
     1801 gcggcgaaga tcgcggtcgg gcgatcgtcg aggtcgagca gtgcattggt cgcggcataa
     1861 ccggactggt aggagaattc gccatccacc accagccgcg cgtcatagcg caagccggca
     1921 cggcgcaggc cttcacggta gcccgccagg cgccacgcgc tggcaccgtg cgcggcgtgg
     1981 ccgcggatgt gggcgatgcg ccgatgacca agcgatgcga ggtgcgccat catcgcgcag
     2041 accgcgccgt gctcgtccac caccacgccc acgtggcggt tctgctcctt cgccgagatg
     2101 ctggaatagg gaatccccag ttcctccagg cgccgcagca gggccacgtc gtcggtgatc
     2161 ggcggggtca ggatcacccc atccagttgc gcctgcatca ccagcgcctc gaccttgtcg
     2221 atgatgtcgt tggcgtcgta caccagcggc gcgagcatca ggttgtagtg ctgcgcctgg
     2281 caggcatcca gcacgcccat ctcgatttcc atcaggtagt tgctcgaggg attgtcgaac
     2341 agcatcgcca ccaggtacga gcgccgcccg gcgagtacgc gcgccgatgg cagcggccgg
     2401 tagcgcagct cctccaccac cgcctcgacc agcgtgcgtg tgcgcacgga tacattgggc
     2461 tcgttgttga gcacgcgcga caccgtcttc atcgagaccc cggcctgctc ggccacgtcc
     2521 tcgatgcgca cccgcgccac cctgatcctg cccgccgctt cgctcgccgc ttcgcccgcc
     2581 ccccgcgttg tgctcatgta ttcgttccgt aatccgttgt ccgacccgcg cgaaagttcc
     2641 cggcgatgcc gcgccgcagg acgatgccat gtgcgcatct tgcctgcgct atattctgca
     2701 ctctggatga caacgctgtc atttcgcgct gtcgcatcgc acagcgcccc tgcctggagc
     2761 cgccaaaaaa gatgagccga cgtcccgcaa acaccctttc gcgcccgcgc cttgccctgc
     2821 tgtccgcgct ggcggcggcc gtgttcattg cgcacgccgc gcccgcgccg caagaggctg
     2881 cgaccggcaa cgccatcaac cccgggcact ggccgcagcc cacctggccg ctggcgaccg
     2941 atgccgcgct ggagcatcgc ctggaggcat tactggcagg cctcacggtg gaggaaaagg
     3001 tcggccagat cgtccaggcc gatatcggct cgatcacccc ggacgacctg cgcaagtacc
     3061 ggctcggttc gatcctcgcc ggcggcggct cggatccggg cggccagtac aacgccagcc
     3121 cgcaggcctg gttggcgctg gccgacgcgt tctgggaagc ctcgatggac acctcgcagg
     3181 gcggcaaggc gatcccagtc atctggggca tcgacgccgt gcacgggcag agcaacatca
     3241 tcggcgccac cctgtttccg cacaacatcg gcctgggtgc cacccgcgac ccggccctgg
     3301 tccgcgagat cggccgcatc accgctgcgg aaacccgcgt gaccggcatg gagtggacgt
     3361 tcgcgccaac cgtggcggtg ccgcaggacg atcgctgggg ccgcagttac gaaggctatt
     3421 cggaaagccc gggcgtcgtc gcgcaactcg cgccggcgat ggtcgagggc ctgcagggca
     3481 aggcgggcac gcgcgtattt ctcgacgaac gccacgtgat gaccacagtc aagcacttcc
     3541 tcggcgacgg cggcaccacc aacggcaagg accagggtaa taccgaagta accgaggccg
     3601 tgctgcgcga tgtgcacgcg gccggctacg tgcccgcgat cgccaccggc gcggcgtcgg
     3661 taatggcctc ctacaacagc ttccatggcg agaagctgca cggcagcaag gccctgctca
     3721 ccgacgtgct gaagacgcgg atgaacttcg gcggcttcat cgtcggcgac tggaacggcc
     3781 acggccaggt gccgggctgc agcaataccg actgcgcgcg gaccttcaac gccgggctgg
     3841 acatggcgat ggcgccggac agttggcgcg ggctgtacga ctccacgctg gtgcacgtga
     3901 aggccggcac cattccgatg gcgcgcctcg acgacgcggt gcgccgcatc ctgcgggtca
     3961 agctgcagat gggtctgttc gagcagccca ggccgtcgca gcgcgcgctg ggcgggaagt
     4021 tcgaactgct cggcgcggcg gaacatcgcg cggtcgcccg gcgcgcggtg cgcgaatcgc
     4081 tggtgctgct gaagaacaac ggcggcgtgc tgccgctgga tccgcgcagg cgcatcctgc
     4141 tgaccggcga cggcgccgac aacctcggca agcagaccgg cggctggacg ctgacctggc
     4201 agggcagcgg caccagccgc gaggatttcc ccaatgccga ctcgatctgg gacggcttcc
     4261 gccagcaggt cgaacgagcc ggcggccacg ccgaactggc ggtggacggc acgtacagca
     4321 ccaggcccga cgtggcagta gtggtcttcg gagaagagcc ctatgcagaa ttccagggtg
     4381 acctgcccaa cctgatgttc aaggccggca ggtccggcga cctggaactg atccgcaagt
     4441 tgaaggccga cggcatcccg gtggtggcga tcttcctcag cggccggccg ctgtggatga
     4501 accgcgagat caatgccgcg gacgccttcg tcgccgcctg gctgcccggt tcggagggcg
     4561 ccggcatcgc cgacgtgctg ttgcgcgacg gccgcggcaa ggtccagcac gacttccatg
     4621 gcaagctgag cttttcctgg ccgcgccgcc ccgaccagta caccaacaat gccggacagc
     4681 cgggctacga cccgctgttc ccgctcggcc acggcctgcg ctacgcggat cgcggcacgc
     4741 ttgcaccgct gtcggaagac cccggcatcg acccgaacga tgcacgcagc aatctgtggt
     4801 tcgaccgcgg ggtcggcacc accgggctga ccctgcagct ggtcggcgcg ggcggcgtgg
     4861 cgatggacgt gatccatcct gccgccgcca ccgccgacgg ttcgctggcg atggaggcga
     4921 tcaataccac catccaggaa ggcgcgcgcc ggttcgactt caagggtgcc ggcgaggtcc
     4981 tgctgcgcgc caacgcaccg ctcgacctgg tgcgcgaaac caacggcgac gtgttcgtgg
     5041 tcgcgacgct gcgggtggac gcgctgccgg caggcggcaa ggcgacgttc cgcgtcgatt
     5101 gcggcgacgg ctgccgcggc gagctgccga tcggcgacac gctggcggcg ctgccgcggg
     5161 gccagtggac ccggctcggc gtccagctgc gctgcctgcg cgaggccggc gccgataccg
     5221 gcaagctgtc cgtgccgttc gcgctgcggg gcagcgccgg cctgcggctg tcgctgtcgc
     5281 aggtggcgct gggcacggcc tttgaccagc gcgtcgagtg ctcgctgcaa taggtgcgcc
     5341 gcacgaccgg gggatcgcac ccccggcttg cttcacctgc ctgcgggatg ccagcggaac
     5401 gtcaccacgc tgccaccggg cagcaggtgg cggaagctgc gcccaccctc gcgcacccgg
     5461 atcgcgcgcg gctgcgggtc ggcattgctg accaccagca cgcggctgtc gtcgtggttg
     5521 acgaaggcga cgttggccac gcccgccaac gcttcactcg atgccacccg ccatgcgccg
     5581 cggcggacga agcggctggc gtgcgccagc gcgtagtagt catcggtgcg cgacacctcg
     5641 ccggttttcg agtcgatggt cactaccccg cggcagttgc cgcagccgcc ggcatgcggg
     5701 ccgctgttct cgtccagcgc caggttccag aacagcaccc cgcgcgccca gttgcgggtg
     5761 gtctcgacga tcagccgctc cacctgcagg gtcagcccgc cgctgcgcac cggctcccag
     5821 tcgccacccg agcattcggt catgtaaacg tcgacatctg ggtgcgcccg ccgcacctgc
     5881 gactgcgcca ccggctggcc ggcgtagcag tgccacgcca cggcatcgac gtaacgcgcc
     5941 gcgaccgggt cggccagcac cgccagcggc tcctcgagct tgtcccagtt gtggtcccaa
     6001 tcgaacaact gcacctgcgg cgcctgccgc gccagcagcg ggccgaggtg gtcaccgatg
     6061 aaacgcgcgc gcgccggcgc attgaggcgc atgcccggat agtccttcga ctcgaagtcg
     6121 ggctcgttct gcacggtcag cgcgcggatc gaaataccct cgcccgccat cgcctcgacg
     6181 taacgcagca ggtagcgcgc gaacgcatcg tagtgatctg gcgacagcct gccggtgatc
     6241 aggtggccgc tgtccttcat ccacgccggc gcgctccacg gcgaagccat gatccacagc
     6301 tccgggttga tcgccaacgc ctgcctcgcc accgggatca ggtcaccacg attgacgtca
     6361 aggctgaaat ggcgaagcgc cggatcgggc tgaccctccg gcggatcgtc gaggctgtag
     6421 tggtggcgcg agaaatccga cgcgccgatg gtgagccgag cgaagtcgaa gccgatgcca
     6481 tcgtcatcac ggccgaacaa ctcgcgcagc agcgcctcgc gctgggccgg cgacatcttc
     6541 tgccgtatca gccaggccga cgcatcggtg atcgagacac cgaagccgac catctgctgg
     6601 tggcgtgcgc tggcatcgat ctcgatcaac gcggcaccgg tggtcgcgcc atccacggca
     6661 tcgcccgcat cgaagcccac ccgcgaaggt gccagcgcca accggtggtc tgcggtggtg
     6721 gtccagacct gcacctggtc cgtgccagga ggcggcgacg ggcgcgcgca gccgcccagc
     6781 atggccgcca gcaacaggct ccagcccgcg catctgcgca cgcgggcgtt tgcgctcacg
     6841 cgaccacccc cggcacctgc gacgcaaccc attggaagaa aacagttttt ttcatgagtc
     6901 ggcacccttg gcacgccgaa tttgacatat ggaatccttg ctttccatta tgacagcgct
     6961 gtcaaaacct ttgggagggg aagaccatgc tgtatccata cacccgcgcc accaggcgcg
     7021 cgacgcaaac cgcggccatg cgccgccaac acgccatcct gccgcgcgcc agcgcgctgg
     7081 cgctggcctg ctccgctgtg ctcgcgttct ccgcgcaggc gcagcaggtg gaaccggcga
     7141 agacgccacc ccaggagagc gccaaaaacc tcgacaaggt cgtcgtcgtc ggcatccgca
     7201 gcagcatcgc cacctcggtt gaggccaaga acgaatcgac ctcgatcgtc gaagtggttt
     7261 cggccgaaga catcggcaag ctgcccgaca tcagcatcgc cgactccatc tcgcgcctgc
     7321 ccggcctgac catgcagcgc ctcgacggcc gcggccaggt gatccacatc cgcggcatgt
     7381 ccgagcagtt cgcgggcacc ctgctcaacg gccgcgaaca ggtgagcacc ggcgacagcc
     7441 gcggcgtgga gtttgaccag tacccggccg agctgatcaa ctcggtcacc gtctacaaga
     7501 cgcccgatgc ctcgctgatc ggccagggcc tgtccggcac ggtcaacatg cagacggtgc
     7561 gcccgcttga cttcaaggag cgcaaagtcg tcctcggcgt gcagggcgac cacaattcgt
     7621 tcggcgagct caccgccggc ggcaaggaca ctggctaccg ggtgtcggcc tcgtacatcg
     7681 accagttcgc taatgacacc gtgggcctgg ccgtcggtgt ggcgcgcctc aacgcgccgt
     7741 tccaggagaa gcactacaag tcgtggtggt gggccaacac cacgctgtgg ggcgcgccgc
     7801 agggcgccaa gccggccgac gcgatcgcgc tgcagggcgc agaggcgtgg gtgaagtcgc
     7861 gcgacaccac ccgcgacggc gtgatggcgg tgctcgagtt caagcccaac gacgcctggc
     7921 acagcgtgat cgatgcctac cactccaagt tcgaccagag cgagtggatg cgcggcgcga
     7981 tgtggaccaa cgacccgtgg tggaacaacg gcgcagtgac ctacaccaac gccaccacca
     8041 ccaactataa tggcaccccg gtggtgaccg gcggcaccct cagcggcatc cggccggtcg
     8101 tgcgcaacga caacaacctg cgcgacgaca gcctgtcggc gttcggctgg aataccgagt
     8161 acaagaccgg agcgttcacc gtggccgccg atctcagcta ttcgcgcgcg gtgcgcgaac
     8221 agtcaactct ggaaacctac gcaggcatga ccagcgggct gaacgtggac ttccgcatcc
     8281 cgctctccgc cggctacgcc tggtacggcc tgcccgacct gtccgcgccc ggcagcgtga
     8341 agctcggcga tccgcagcag tggggccatg acggacgcat ggagaattcg cgccaggtgg
     8401 acaccatgaa ggcgttccgg ctgtcgctga accacgccct cgattccgcc ttcctgtcca
     8461 gctacgacgt cggctacaac ttcagccgcc gcagcaagga caagcacgcg gacgtctact
     8521 tcgccgacct gcctggccgc gtcccggtgg cggtggaccc gtcgctgctg cagtcgccca
     8581 ccgacctcgg cttcgccggc atgggcagcg tgctgaccta tgatccgcgc gcgctgttgt
     8641 cgaactacta caacgtgtat ctgagcgaga ccaacgacga cctgcagaag gacaaccgga
     8701 tccaggagga gatcggcacc ttctatgcca aggccaacat cgacacgacc ctcggcgagc
     8761 gggtgaacct gcgcggcaac atcggcttgc agtacgtccg taccaaccag aagtcgaacg
     8821 gcgtcggcat cacccccgcc ggcccggcag cggtgatcgg cgtgggcacc acctacaacg
     8881 acgtgctgcc cagcctcaac ctggtgttcg acttcaacaa cggctggaac ctgcgcttcg
     8941 gcgccgccga ggagcagatg cggccgcgca tcaacgacat gggtgcatac gccaacgtcg
     9001 gcgtcagcgt cggcaacggc gtcgcgcagt ggtcgggcag aggcggtaac ccgaagctgg
     9061 aaccgttccg cgccaaggcg gtcgacatct cggtggagaa gtacttcggc ccggccagct
     9121 acatcgcgct ggcggcgttc tacaaggacc tggagtccta cgtctacacc agcaccattc
     9181 cgtgggactt caccggttac gagttcgaca cgccgatccc gccgcagtcg aacatcggca
     9241 ccttctccac cccggccaac ggcaagggtg gctacatgcg cggcgtggag ttcagcaccg
     9301 cgctcggcgg cgaattgctg catccggccc tgtcgggctt cggcctgctg ctgaacggct
     9361 cgtacaccga gtcgtcgatc gatccggacg gccccggcgg ctccgccacc gacaccatcc
     9421 cgggcctgtc gaagatcgtc gccaacgcca ccgtgttcta cgagaagaac ggcttctcgg
     9481 cgcgcgtgag cgagcgcttc cgcgacccct accgcggcga gtacagctac atcttcggcc
     9541 agcgcagcta ccgctacacc cgcaacgagc gcaccatcga catgcagctc ggctacgact
     9601 tcccggaggc cagccggatg cgtggcgtgt cgctgctgct gcaggtgaac aacctcaaca
     9661 acgagccgtt ccgcacccag gtcagcgatt ccaatgggtt gggtctgatg ttccccgagg
     9721 agtacaccga gtacggccgc cagtacctgc tgggcatgcg ctactcgttc tgaggcaagc
     9781 cgcgcgcggc ggctcgtcgg caacggcggg ccaccgcgcg gggctacagt ggcgctccca
     9841 cgttgacgga gcgccgcgat gatcgacctg tactactggc ccacgccgaa cggacacaag
     9901 atcaccctgt tcctggagga ggccggcctc gactacacga tcaagccggt cgacatcggc
     9961 aagggcgcgc agttcgaagc cgattacctg cggatttcac cgaacaacaa gatgccggcg
    10021 atcgtcgacc acgcgcccgt cgacggtggc gcgccgattt ccgtgttcga gtccggcgcg
    10081 atcctgctct acctcgccaa caagaccggg cgcttcctcg gcatcccgga cggcgccgat
    10141 gccggcaccg cactgcgcca gcgcatcctg gtcaacgaat ggctgttctg gcagatggcc
    10201 ggactcggcc cgatgaccgg gcagtacggc cacttccatg tctacgcgcc ggaagacatt
    10261 gcgtacgcga aggatcgtta ccgcaaggaa gccgaacgcc tgctgggcgt gctcgacaag
    10321 caactcgaag gccgcacctt catcgccggc gacgactaca gcatcgccga catggccagc
    10381 tatccgtgga tcggtcccta cgacaaggcg ccgctggacc ttgcgccgtt cgccaacgtg
    10441 cagcgctgga gggcggagat ccgcgcgcgg ccggccacca aacgcgcgta cgcgctgacc
    10501 aggcaggtca gcccgaatgc gggcaagccg ctgagcgacg aggaaaagaa ggtgctgttc
    10561 gggcagggcg cgcgctgacg caacgaaaag gagacaaagg aaaagggggt cagagtggct
    10621 tttcgcgagc gataaagcca ctctgacccc cttttcctga cagccctaca tcttcggctt
    10681 cagctcgaat tccttcggcg gcgtcgcacc gagcaggtgt tcgacgaagt agtcccagcg
    10741 acgacgcatg acgtagtagc tgtccctgcc gtagccgtgg cgcgcatgcg gcagcaacag
    10801 caggtcgaag tccttgttcg ccttgaccag cgcatccacc accagcaggg tggattgcgg
    10861 cggcacgttg tcgtccatca ggccatgcag caggaacagc ttgcccttga ggttcttcgc
    10921 gtaggcggcg ttgtcctggc cggtgtagtt gctggtgccg tcggccttgg tctccagcag
    10981 gccgtggtag cgctcgcccc agtcgtcctc gtaggccagg ttgtcgtggt tgccggattc
    11041 ggcgatgccg accttgtaca cgtccgggta gcggaacatc gcggtcgcgg tggcgttgcc
    11101 gccgccggaa tggccccaca tgccggcacg ggtcgcatcg atccacggat atttcgcggc
    11161 gagttccttc agcgcggcga cctggtccgg cagcgtgttg tcggccatgt tgccgtacca
    11221 cgcgtcctgg aaggacttgc tgcgcagcgg cgtgcccatg ccgtcgatgg cgaccacgat
    11281 gaagccgagt tcggccagcg cctggtggtc ggagcgcgac ggggagaaac cgcggcggcc
    11341 gaccgaaccg acctgcggtc ccggatagat gtaggtgacg atcgggtact tcttcgccgc
    11401 gtcgaaggtc gagggcttga acatcaggcc gtacaggtcg gtcttgccgt cgcgggcctt
    11461 gacgacgatg tcctcgggcg ccacccagcc cgcggccttg aggcgcgtga tgtcggcgcg
    11521 cgcaagttcg gcgacctgct tgccggcggc atcgcgcacc accgcgaccg gcgggttgcg
    11581 ggtcgtcgag tacacatcga cgaaatagcg ggcgtccttc gacggcgtga tggtatgggc
    11641 ggcattttcc ggcgtcagca actgcacgtc gccaccgtcc atcccgacct tgtagaagtg
    11701 cgcgaagtag ggatcgcgac cggcctcgcg gcccacccct tcgaaccaca gcgtgcgcga
    11761 cgccgcatcg atgtgcgcga ccttggcgac attccagttg ccgctggtga gctggcgctt
    11821 ttccttgccg ctggccaggt cgtgcaggta cagatggccc cagtcggatt tctggctgaa
    11881 ccacaggaac tcgttgctct ccggcaggta gcgccagttg accgaatcca gcgcaggcgc
    11941 gctctggtac tgggtggcca cggtttcgct gaacacgtcg cggaccttac cggtcgcggc
    12001 atcggccacg cgcagggtcg cggacttgtg gccgcggtcg gtgctgacga aggccagggt
    12061 cttgccgtcc ttcgcccact gcacgtcttc ccagccctcg ctgcaggcga tgtcgtcgct
    12121 gcaggtggag cggtgctggt ccggcgggag ttgcaggcgg atcgtcttcg gcttggccgc
    12181 cgacaggtcg atgatcaccc gctcgatcat ggtgacgtcc ttgtcgccca cgaacgggta
    12241 cttccactgc tcggccttcg gcgcgccggc gctggtggcg acaagggtca tcgtgctggt
    12301 cttgcgctgg tcctgctgga aggtggcgat cctgctgccg tccggcgacc acaccaggat
    12361 cgcctcgtcg gtgtgcttcc agccggcgtt gtcggtggcg tagccgtaat cgggcttgcc
    12421 gtcgaaggtg agctgggttt ccttgccgct ggcgcgatcg cgcagccaca ggttccaatc
    12481 gcggatgaag gcttcacggg ttccgtccgg cgaggccgcg ccgggctcct cgcccgaggc
    12541 cggcttcgcc agggcaccgc atccggcggc atcgcagcgg aactcgccct gcatgccggc
    12601 caggcgcagg ctgccatcgg cattgcgcac gacccgcagc ggcggcagct tgcccgcatc
    12661 caccggccgg cccttgccgc ccgcggccag gttcatcgcg tcggccagcg cctgcgcgtt
    12721 gaacgcgggc gtggtcttgc cggtcgccgg gtcgaggcgc aacacgcgct ccacgccgtt
    12781 gctgcgctcg cgatagacca gggtgccgtc gtccagccag ccgatgccgc tggtcgcatg
    12841 gtccaccagc ggggaggtgc ggtcgccaag catgctcgcg gcgcgctcgt agtcagccac
    12901 ggtgacctgc ggcgcctgtg ccatcgcgct gcccatgccg acgccggtca ccagcatcgc
    12961 ccccagccaa gcctgtttcg catcgatccg ccccatctgt cccgtcccgt tcccggctgt
    13021 tgcctgaacg ccgatgctag cgcgccacgc cgaccgcggc ccgtgacgaa tggcatggaa
    13081 catgccgcca acagatgcca ctggcatact cgcgccagat gaacgaccaa cccctgccgc
    13141 tgacccccga gcggatcgcc gtggtcgtgc cggccctcaa cgaagccctg cgcatccgcg
    13201 acgtggtgca gggggcgctg cggcactgcc cgaacgtgat cgtggtcgat gacggttcgg
    13261 acgacggtac cgcggacgca gtcgccgacc tgccggtggt gctgttgcgc cacgccgccc
    13321 gcatgggcaa gggcgccggt ttgcggaccg gcttcgctgc ggcgctgcag cgtggcttcg
    13381 cggcggtgat cacgatggat ggcgatggcc agcacagtgc cgacgacatc ccgcgcctgc
    13441 tcgccgcggc caatcgccgg cgcggctgga tcgtgatcgg cgcacgcctg cgcaagcgcg
    13501 ccaaccagcc gctctaccgg aggttggcga acgaattcgg cgactggggc atcgcctggg
    13561 gcaccggcta ccaggtggcg gacagccaga gcgggcaacg cctgtatccg gccgacgtct
    13621 gcgcactggc ctgcgccggc atccccggcg aagagttcgt gttcgaggcc caggtgctga
    13681 tgtccgccgc gcaggaactc ggcacccgct gcgtgtcggt gccgatcgag gcccgctaca
    13741 acgtggtcca cgctgcaccg gacttccgcc ctagccattt ccgcccgttg cgcgacctgt
    13801 ggcggatcac cagccacatc gtcctgcagt gcctgcgtcg tggcggcctg tggcagatgc
    13861 accggcgcgt gcgcgccaat ccgccagtga tcgacgaccc cgccggcgag ttcgccaccg
    13921 acctggcggc ggcacagccg cgctgacccg gcacccgcga cccgcgcgct atcatccgcg
    13981 ccccgatgcc gtcacttccc gagcccctgc cgcatgctgc cgccacgctc accgtggagt
    14041 accggcacga tccgctggac gccatgctgg ccgacccccg gctgctggcc gtattcggtt
    14101 tcggcgacca ggccccggcc atgcacggcg acccgcgcta cctgcgggtg gacctgccgg
    14161 tgcgtggcga cgcgccattc gaatgctggc gggtggacaa cgaggtccat cacggtcgtc
    14221 gcgacggcat cgcctggagc gaggacggcc acctgcagtt cggcgccctc gacgtggccg
    14281 atgccggcga cggcgacatc gaacgcgccg ccgagcaggc gtacgcgcgc ctgcaggcct
    14341 ggctggccgc aggcgactac ccgcacccgc tgcggatctg gaattacctg gacgcgatca
    14401 ccgccggcga aggcgacgac gaacgctacc ggcgtttctg cgtgggtcgt gcacgcggca
    14461 ttggccgcgc gctggggccg gcggaattgc cggcggcgac cgccatcgga cacccgcgcc
    14521 ccagtggcaa gctgcaggtg tactggctgg cggcgaagac gccgggcacc ccgctggaga
    14581 acccgcggca ggtgcaggcc tggcgttatc cgcgccagta cggcccgcaa gcgcccggct
    14641 tcgcccgcgc gatgctgccg gcgtccatcg cgatgccgct gctggtctcc gggaccgcgg
    14701 cggtggtggg ccatgcctcg cagcacggcg actccctgca ggcgcagttc gaggaagtgc
    14761 tggccaacct gggcagcctg gtcgaacgcg cgcgcgcgct gcggcccggc gtaccggacc
    14821 ggccgggcaa cggcacccgc ctcaagatct atgtacgcga cgccgatgcc ctgccgacgg
    14881 tcgatgcgct gctgcggcaa cgcttgcccg gggtgccggc gctgctgctc catggccacg
    14941 tctgccggcg cgaactggcg gtcgagatcg acggcgtgca tgcttgagcg atccgcccgc
    15001 gcagggcaac gcgacacgac cggagatcat cgcaatggga ctgatcagca tcctgtgggg
    15061 catcgtggcc atgggctgga tggtgctggc gctgatcccg ctggtcggca tcaccaactg
    15121 gctgctgatc ccgttcgcgg cgatcggggc gatcctggcc gcgatcggca tcgcggtgac
    15181 ctccgcgggc aagcgtggcc gcgccaaggc cgggctgatc ctgaattgcg tggtgatcgt
    15241 ggtcgcgacc tggcggctgg gcctgggcgg cgggctgatc tgaccattcg ctactgaatc
    15301 gccagctgaa ccggcaacat gaacgcgggt tgaccgttga gacggccagc cgtttgtcgt
    15361 aacgtccacg ggtccgacgg tcgcggcagt ccgtcggggg ggttctgacg aatacgcgtc
    15421 acggacgaaa gcgctgccgt gcacatgggg gttcataaat gtccagcaac aacaagatca
    15481 ctggcgcgct ggcggcattc gccgtcgccg cgctgatggc gggcccggcg gtcgccggca
    15541 ctccgctcaa cgtcgtccag aagaaggccc tggcggatgc gaaggcgttg caggccccga
    15601 gccccaccgc cggccagacg ctgaccggtg cggacggctc gcccgctgcc gttgttccgg
    15661 atgagctgca caactacatg cacgcacagc gcgacgccag cggcaaggtg caggtcctcg
    15721 agaccgacgg cacgactgcg ccggccaagg ccgtggaggt gaccaatgaa taagttgcgc
    15781 aacgcactcg cggttgccgt gctggcagcg ctcgccgccg cctcttccgc gcaggccgcg
    15841 gacctcatcc cggtcaacct ggacggcgcc aacgaaggct acaacgacaa cacgccggcc
    15901 acgccggtcg gcggcaacta cggcacgacc aagggcgaac agcgccgcat cgtggcccag
    15961 tacgccgccg ccatgtgggg cagcgtgctg aagagcgacc agccgatcta catcgcggcg
    16021 cagttcaatc cgctgggcgc caacgtgctc ggttccgcgg gtgcggtgac cgtgcatgcc
    16081 aacttcggca acgcgccggt cgccaacgtg tggtatcccg ccgcgctcgc cgacgcgatt
    16141 tccggcatcg acctggcacc caccagctac gacatcagct cgcagttcag ctccgacttc
    16201 agtttctact acgggctgga cggcaagacg ccggcggggc agatcaactt cctcgacgtg
    16261 gtgatgcacg agttcggcca cggcctcggc ttccagaact tcgagaccga agcgaccggc
    16321 gcgttcctct ccggccgtgc ggacatctac tccgtctaca cctacgacaa caccaccggc
    16381 aagttctgga ccgcgatgac gcaagcggaa cgcgtgacgt cggcgctcaa ctacggcaac
    16441 gtggtcttca ccggcgcgaa tgccacggcg ggtgccaagc tgctgctgga cgaccgcaag
    16501 gacttccgcg tcaccgcacc cgctccggtg gccggtaact acgccatcgg cagcgttacc
    16561 tttggtccgt tgatctcgac cacgaacttc aatggccagg tggtgctcgg caccgatagc
    16621 gtcggcaccc ccggcgacgg ctgcgaggcc atcaccagca acgtcgccgg caagatcgcg
    16681 ctgatcgacc gtggcgtctg cacgatcgcc atcaagacca agaacgcgca ggacgcgggc
    16741 gccatcggcg tcatcatcgc caacaatgcc gccgccaacc cgccgccgaa tttctccggc
    16801 gtcgatccca ccatcaccat cccgacgatc gtcgtcaccc aggccgcagg cacgtcgttc
    16861 aaggccaacc tgccgatgac ggtgacctac gtggtcgatg ccaccaagtg gcagggtgcg
    16921 gatgacctgg gccgcccgcg cctgttcatg ccgaacccgg tgcagtccgg ttcttccggg
    16981 tcgcactacg acaacaacgc gatgccgaac ctgctgatgg agccggcgat caacgactcc
    17041 ctgcgcagct actacaacct cgacatcacc ccgcacctgc tcaaggacat cggctggcag
    17101 ctcaacaccg ccagcgcgaa gatcggcagc ccgaacgtgc cggactgcga caccggcctg
    17161 tcgggcaccc cgtccggcgg cctgttcgtg gccggcacca tccaggccag caacgagctt
    17221 tgcctgatca gcgcacagac ccgcggcgcc tactacagct gcatggatgc cgcgcgcgac
    17281 cgcatggtcg ccgccggcgt gctcaccagc ctgcagggca acaaggtgat gatgtgcgcc
    17341 aggcgcgtgc aggaccacca ggtgttcccg atcgcgttct gatcggttcc gcgcaccacg
    17401 cagtaccggc cggcgccttc gggcgccggt ttttcttggc gggcgtaaac tggcggccat
    17461 gacatcaccc gccgcccaac gcttccccgt ccgtccgcgg cgcatgcgcc acgacgcctt
    17521 ctcgcgccga ctgatgcgcg agcacgtgct caccgccaac gacctgatct acccggtctt
    17581 cgtccacgaa gaaacgggcc gcgccccggt cggctcgatg cccggggtgg aacgcctgtc
    17641 gatcgacgaa ctcctgcgcg tgggcgagca ggcactggaa ctcggcgtgc cggtgctgga
    17701 cctgttcggc gtgccggatc cgtcggcgaa gaccgcggac gggcgcatcg cctgggacga
    17761 ggacggcatc atccagcgcg cgatccgtgc gctgaaatcg cgcttcccgg aactcggggt
    17821 gatgagcgac caggcgctgg acccatacac cacgcatggc caggatgggt tggtcgacga
    17881 ctccggctac atcctcaacg atgagaccat cgaagcgctg gtcaagcagt cgctgtcgca
    17941 tgcggcggcc ggtgccgaca tcctctcgcc cagcgacatg atggacggcc gcatcggcgt
    18001 gatccgcgac gcgctggaag ccaacggctt catccacacc cggatcatgg cctacagcgc
    18061 caagtacgcc agcgcgttct atggcccgtt ccgcagcgcg gtggggagcg ccgcgaatct
    18121 cggcaagggc aacaagtaca cctaccagat ggacccggcc aattcggacg aggcgctgca
    18181 tgaagtcggc ctcgacctcg ccgagggcgc cgacatggtg atggtcaagc ccggcctgcc
    18241 ctacctggac gtgctgtacc gggtgaagca gcacttcaag cgcccgacct acgcgtacca
    18301 ggtcagcggc gagtacgcga tgatcaaggc cgccgccgcc aacggctgga tcgacgagaa
    18361 gatggtggcg atggaagcgc tgaccgcgtt caagcgcgcc ggcgcggatg gcatcctcac
    18421 ctatttcgcg ctggacgcgg cccgctggtt gcgcgaaggg cactgaccac cgcatcgagc
    18481 cacgggagac ctgcatgacc aggcattacg ccgtcttcgg cacaccaatc gcgcactcgc
    18541 tgtcgccgcg catccatgcg gcgttcggca agcagaccgg catcgacctc gagtacgtgg
    18601 cgatcgaagc cgggccggag gacttcaccc ggaagctgga cgccttcgcc gcgcgcggcg
    18661 ggctgggcgc caacatcacc ctgccgctca agcgccgtgc cgccgaactc gccgcgacgc
    18721 tgggcgatcg cgcgcgccgc accggcgtgg ccaacaccct ggtccgccgc ggcgacggct
    18781 ggcatgcgga caacaccgat ggcagcggcc tggtcggcga cctgaccctg cgccaggggc
    18841 tggacctgcg cggacggcgc acgctgttgc tgggctcggg cggtggtgcg cgcgggatcg
    18901 cgccggcgct gctggatgcg ggcatcgacg cgctgtacat cgtcaatcgc acggcacaga
    18961 acgccgacgc cctcgccgac ctgctcggcg accccaagcg cgtgcatgcg cgctacctcg
    19021 acgacctgcc ggcactgggc gaattcgacc tgatcgtcaa cgcaacctcg gccacccgcg
    19081 gtggcgcgtt gccgcacctg ccgatgtcga tggtcggtcg ccgcaccgcc gcggtcgacc
    19141 tgagctacgg cgaggccgcg atcccgttcc tcgcctgggc gcgggcgaac cgctgccatg
    19201 acgcgatcga cggactcggc atgctggtcg agcaggccgc cgagagcttc gaggtctggc
    19261 acggcgtgcg cccggacacc gaccgcgtct acgccgacct gcgtgcgcac acggattcgc
    19321 tggtgacggc ggactgagcc aggcgcgggg cggctacagc aggccgtcgc gcttgatcgc
    19381 caggtagcgt tccaccagcg cgcccgggag ttgctcacag gtcacgtcga gcacgccgac
    19441 ccgatgcgaa cgcagcgcat cgtgcacggc ggtgcgctgg gcgatatagc gtgccgccgc
    19501 gcccgcgcgc aatgcgcccc gcaggtcgcg cacgtcctcg tccagcacgg cgtccagcgc
    19561 ttcctcgcgc aggctggcga cgaccacgtg gtggcgctgc tggagcatgc gcaccgcgcc
    19621 gagcaggtcg tcgctgtctt catcgcgcag gttggtgacc agcatcacca ggctgcgccg
    19681 gcggtgccgc tgcgacagtt cggtggccgc agccaggtaa tcggtcgcca ccggccgcgg
    19741 ctgcaggtcg taggtggcgc gcaggaacac gtcgatcgcg cccatcccgc gctgcggggc
    19801 gacccagcga cgctcgccgc cgctggccag catgccgacc gcgtcgccct ggcgcagggc
    19861 caggtaggac accaccagcg cggcgttgag gacatggtcg aaatggctga gttcgccccc
    19921 cgccttcgcg agggcaggct cgcgggcgag catccggcgg ccggtgtcga tcaccatcag
    19981 caggtgctgg ttgcgttcgt cctggtactc gcgcgagatc agccggcgca tccgcgcggt
    20041 cgccttccag tcgacctggc gcaggctgtc gccgacccgg tagtcgcgca tctggtggaa
    20101 gtcggtgcct tcgccgcgcc gccgcttcac gtgcgcgccg accgcgcgcg aagcggtctc
    20161 ggcgccgagc aaggcaagcc gcgccaacgg cgcgaaatcc ggatacaccc gcaccgtgcg
    20221 ctgcgcgccg accacgcgcg cgcgcgtcca caggcgccaa ggcgaatgca ggcgcaggtg
    20281 cacgccgtcg aagcgcgcat cgccgcgttc ggtgggccgc agggcatagt cgaaccgggt
    20341 actggcatcg gcggccagct gcagccggcg tggcaggccg gtggcgatcc atccggtcgg
    20401 gtgcaggtcg tgcacgtcga cccgttgcgc ccgcgcaccg gcatccagca ccagggtcac
    20461 gttgcgttcg acgcccagcg gcaacgcgtc gggcaggctg cgcgccacct ccggcagcgg
    20521 ctgccggacg atgcgcacgg catcgaccag cgcaaccagc agcaggagcg cgcccgccgc
    20581 ccaccacgcg gccgccggca gcacgccgaa gctggtcgcc aggcccagca aggcccaacc
    20641 gacgcagcac agcaccaggg gcagcgtcgg cctcactggc ggggcgcgtc caccttggcc
    20701 agcaacgcat gcagcacgtc gtcggccgac tggccttcga tctgcaactc cggcgccagc
    20761 gacactcggt ggcgcagcac cggtttcgcg atttcacgga ggtcgtcggg ggtgacgaag
    20821 tcgcggccgc ccagcaccgc ctgcgcacgc gcggcacgga ccaggccgat gctgccgcgc
    20881 gggccagcgc ccagcgcgat gcccggccat ttgcgggtgg cggcggcgat ccgcacggca
    20941 tagtccagta ccgccgggtc gacacgcacc tgcgccgtgc ccagctgcat cgccaacacc
    21001 tcgtcctggc tggccacgcg ctggacctgg cccaggtcga aatcggaggc catccgaccc
    21061 tcgctgaccg cggcgaccat ccgcgtctcg tccgccaggc tgggatagcc gatcagcacc
    21121 ttcagcagga agcggtcgag ctgcgcctcc ggcagcggat aggtgccttc cagttccagc
    21181 gggttctggg tggccaggca catgaacggc ggcggcagcg ggaagctggt gccctcgatg
    21241 gtgacctggc cttcctgcat cgcttccagc agcgccgact gcgtcttcgc cggcgcgcgg
    21301 ttgatttcgt cggccaggag gagattggtg aacaccgggc cccggcgcac ctcgaagcgt
    21361 tccagcttcg gatcgaacac cgcatgcccg ctgatgtcgc tgggcatcag gtccggggtg
    21421 aactgcacgc gcgcatgcgc gcaacccagt gcctgcgaca gcgcgcgcac cagcagggtc
    21481 ttgcccaggc caggcacgcc ctccagcagc acatggccgc cggcgagcag ggccaccatc
    21541 acctggtcca gggtgtcgtc ctggccgatg aaggccttgc cgacttcggc gcggatcgcg
    21601 ctggcgcgtt cgcccaacgc ctcgccggcg agcggtgcga tggtcggggc gatgtcgatg
    21661 ctcataggcg tttcctcagg tcgatcaggc gggcaatgcg atggcggaat tcggtggtgt
    21721 tgaccggcgg gcgcgtatcc agggtggcgc gcacctctgc agcggtcatg gcgacgcgcg
    21781 acgcgaccag tgcggcctgg gtctcgctgt cctgcgcggc ggccagcggg tcgcggcggc
    21841 gcaggcgcgc cagcacggca tcgcgcaacg cgcggtggag cacgccgagc ttgccgtagc
    21901 gcagcaggtg ttcgccgctg gcctcgacat gctccagcag cgaacgccgc ggcggcatcg
    21961 gcgatggcag cagcgggccg aagcgctgca tgcgcatcca caaccaggcc agcaggccca
    22021 gcagcagcgg cagcaaaaac gcccacgcat gcgccagcaa ccagcgccac agcggcggca
    22081 tgtccgcggc atagaccagg tggaagcgac ccttgcccca gttcggcgcc agcaactggc
    22141 gggcgaaggc caggtgcggg ccttcgtcca ggctctggtt gtcgatcatc tccatgtcgc
    22201 cgagcaggtc gacgctgcca tcgccatacg ggaagcgcgc gaacacgtgg tggccgtcgc
    22261 gggtgcgcca cgccgcggac acgtcggcgc catcgaccag ctcgaagcgg ggtgccccgc
    22321 agaattcgac atggtcctcc tggccgggga cgaacaggtc catgcagtgg ggatcgacca
    22381 gctccggttc gatcggcagc cagtccgcca gggcgccggc aaaggactcg tccggctgcc
    22441 acgacggcag gcgcagcacc aggtgcccgc cttgttcggc gaactcgaac agtgcctcca
    22501 cctggtcgtc gccgagcagg cgcggatcgc tgtagaccag cagggtgtcg ccggcggcca
    22561 gggccatctc gtccagttgc aggcgctggc gcgattccac gcgctggccc tcggcgcgca
    22621 gggcgagctt gagggcgtag agcgggttgt aactggcctc gccgcgcggc ggcatcggca
    22681 cgatcttctc cacgcggtgg tgggtggcca ggaaccaggc cacgccggcg gcgatgacgg
    22741 ccagcgccag cagccacggc aatgcggcgc gcgcgcggct catgcggccc acccgaagcg
    22801 cccgcccagg cccgccagca ggccctcgaa ctcgccgtca tcgggcagcc gctcggcgta
    22861 ggcggcgtac tgccacaccc gcaccatccg accgaaggca tcgcggtcgg cctggtcatg
    22921 caggccacgc gcggcgcgca ggcattgcgc ctcggtcgcg cccggcacca gcacggtgcc
    22981 ggtgcgcgcg accatgccgg ccacgctggc gcgatacagc aaggccaggg cacgccgctt
    23041 gcgcccgtcc tgccagagct ggcgcgcagt gccggcgata tcgtccggca acgcgtcggt
    23101 ggccaggtgc ggctcgctgg ccacgggcgt ctccgcctgg cgcggcggcg gcgcgatccc
    23161 gcgcagccac ggccaccact ggcgatgggt caccagcagc aggcaggccg cgaacgccgc
    23221 cagcagccac agtccgtatt cgccgatcgc cgcgatgatc ttgccgatga tggccgggaa
    23281 ctcacccggc ttgggcggct cgccgggttt cggcggcttg cgtcgttccc aacgggtttc
    23341 cttgcgcttc ggcgagagca gcggatccgc gtaggcgcgt ttgaccgcct tgtcgaaacg
    23401 ggccggatcc tgcagctgcg ggaacaccat cggcagggtg ggcggctgcg aggacttggg
    23461 tgccttgacg gcaggcgttg cgggtgcggg cctggctgcc gcatcgtccg tgggcgtcgg
    23521 cgccggcggc acttgcgcct gcgccggcct gggcatcgac aggcccagcg cgcaggcgac
    23581 cagcaacaac ggcagcagcg cccgcaggcg gtgccgcatg cgccggaacg cgatctcgat
    23641 gtcccagcct tcgatctggg tgcgccggtc caggtacagg ccgaagccgg cacccacgta
    23701 gaacggctcg accaggctgg tcgccagcca ggccactgcg ttgccggcga cctgcaccca
    23761 cggcggcggt tccacggtca gcagggccca catcgcccgc gccgattccg acatgaactc
    23821 gaccggcacg aacaccatca ccagcgcata cgcgccgaac agcagcgcgg cctcgaagcc
    23881 catgcagacc agggtcgaga ggaaggcggt gccccgcacc gaaccgccaa tcacccgccg
    23941 acggggtccc ggattggcgc cgccttccag caggtcgacc ggcaggtaca gcgcgcgggt
    24001 cggccccagc cgccgccagg tcaggtaggc cggcatcgcc cgccatcccc aggtccgctg
    24061 cgccgccagc gtttcacgca cgccgggggc ctcggagaac agggcgcgcg acagcacgaa
    24121 cagcgggatg cggtcgaaca ccggcttcaa ccaccacatc gccaccgcca cccacggcag
    24181 gagcccggtg aaccagccga tcaccgtcaa caaggcgaac accggcagcg tcaccagcag
    24241 ccacggcaac cagatcgccc gcgcgtgggt gcgcaccagg gcggtgccca gctccatcgc
    24301 ctcccacggc gaacgcgggc gcaactccac ccgcggctgg tcaaggcgca tgccggtccc
    24361 tcccgccgaa ccacagccac gcgagcacca gcgcccacag cacgccgccg accgaatact
    24421 tgccccaggc cgggatccag ccgatcgacg accagaacgc ctcgatgaag gccgcggcca
    24481 ccagcatcgc gaacacgccc aggcacagcc gcgcgccgcg gcgcccgccc tcgaccagcg
    24541 cgtccacgcg ccggcgctgg ccgggcgcga tcaggtccag gcccaggcgc aggcccgccc
    24601 caccggcgat cacgatcgcg ctgagttcga acgcggaatg cccggcgacg aagcgccaga
    24661 acggctcgcc atgcccggtc gcctgcaggt ggccggccac gccgccgatg aacaccccgt
    24721 tgaaggccag cgccagcgcc gggcccaggc cggccagcag gccactggcg aaggtgcgca
    24781 ggccgatgct gatgttgttg aagatgtagt agccgaacat catcaggtcg gattcggcgg
    24841 cgcgacccag ggtgctcttt gccgggtcgt acatcgactc gacctcagcc acctgctgcg
    24901 cgcccatcag ggtgtggatg agctccggct tcacctgcac gccgacgtag ctggccacca
    24961 gcgggatcgc gaacagcgcc agcgccgccc acatcacccc ggcctccgcg cgcaccagcc
    25021 gtggcatgtc ggccagcagg aactcgaacg cgcgctgcca gcgcggcagc ggcggccggt
    25081 acatcaccgc atggccacgc tgcatcagtt cctgtaggcg cgcgaccagt tgcgggctgt
    25141 aaccgcggcg gcgggccagg cccagttgct ggcagacccg gcggtaggcg gccggcacgt
    25201 cctcgtcggg caggccgcgc cagctgcggt cggcgcgcgc gcgccgcggc cgcgcgccac
    25261 gcgcatccag ccattgctcg aaggcctgcc attcggcctc gtggcggacg atgaagtgtt
    25321 cctgccgcat ccgggcctca gcgcttgccc agcagccagt gggcgatgcc ggccagccgg
    25381 cgcacgccct cgccaccctg cgccccggtc gcgggcgcgg cgatgtcggc cagctcctgt
    25441 tgccgctccg gagtgagccg cggcgcgcgc tcggcgaagg cgacgatcgc cgcctgctcc
    25501 tcgggccgca acggcaacac cggcgccatc accccgtcgg cgaccatcac cgacaacggc
    25561 cgggtcttcg gcacgtgcac gaccatggtg ccggcgacca ggtcgcccag ccgccgaccc
    25621 cacgggtcga acaacccgct gaccagcccg caggcgtagc cgaacggcag catgtccacg
    25681 gtgcgcatca ggttgcgcac gcaggtggcc agccaaccga ccggcgcgcc gtcgctggac
    25741 accacgcgca ggtccagcgc gcgcttgccg ggcgtctgcc cgtcgaacag cccctcgcac
    25801 aggatcggat acagccagaa cagcgcgaac aggaccagca ggtagacgcc caccccggcc
    25861 ttgtcgagcg cacccagcac catcgatgcc gccagcaaca ggcccatgcg gatgccgaag
    25921 tcgatcagcc acgccaacgc gcgcggcacc ggccccgccg ccggcagtcg caacgccacg
    25981 ccttcgggcg tatgcacctc gcggacggta tccagcatca ggcgtcgacg acgatggtgt
    26041 ccgcgatcag gtcgtggatg cagcgcttgg catcgccgaa gatgaacagg ctgtccagca
    26101 gcgtgaacag cgggccgacc agcgggatgc cacccagcat gcccggcacg aaataacgca
    26161 ggccgaggat ccggcccagg ctggcgcgcg tgccgtcgtt gcgcacgatg cggatgccca
    26221 ggaccttctt gccgaaggtc tggccactgc gatgcaactg caccagctgg tagatcgcga
    26281 aggcgatcat gcccaccacc attgccacca ggatcaccat gccgatgggg gaaatgccct
    26341 ggccattggc gttctgggtc gcgggcaagg cgatcgcggc gaggatcgca agcaccacca
    26401 ccggcagcga atccagcaac accgcgccca ggcgcacgcc gcgggatgac ttcatctgtg
    26461 cctggccatg gacgatgtcg gccacccgtg catcgggtgc ggcatagggg ttggcgggtg
    26521 gcgcggaagg cgcctgtcca tcggcgctgt tgtacggatc catgtgttgt ccttcgctgc
    26581 ggggt
//
LOCUS       DAOOCX010000021        26171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1550_length_26171_cov_8.338107, whole genome shotgun sequence.
ACCESSION   DAOOCX010000021 DAOOCX010000000
VERSION     DAOOCX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 26171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26171
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1550_length_26171_cov_8.338107"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1068)
                     /locus_tag="PK399_03455"
     CDS             complement(115..1068)
                     /locus_tag="PK399_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HPW12210.1"
                     /translation="MAARIPTLDIRRFTHPASPADRQAFIDELGAAYREWGFAGIRNH
                     GIPQAQIDEAYATFRAFFALPEETKKKYHVPGGGGARGYTAFGVETAKGAKYSDLKEF
                     WHIGREIPRDSKYAADMAPNLWPDEVPGFQRVGYGLYESLDALGSQVLSALALHIGLP
                     EDYFADKTDSGNSILRPIHYPPITTDDIPNVRAGAHEDINLITLLVGASAAGLEVLSK
                     KGEWVPFTSDADTIVVNIGDMLQRLTNHVYPSTTHRVTNPPGEAARQPRYSVPFFLHP
                     NPDFLIDVLPSTITPDNPSRYPEPITAQGYLEERLREIKLK"
     gene            complement(1136..2497)
                     /gene="glmM"
                     /locus_tag="PK399_03460"
     CDS             complement(1136..2497)
                     /gene="glmM"
                     /locus_tag="PK399_03460"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPW12211.1"
                     /translation="MSARRYFGTDGIRGRVGEGAISADFVLRLGNAYGHALVAARDAD
                     REWRNPMVVIGKDTRISNYMFEAALEAGLVAAGVDVQLMGPMPTPAVAHLTHSMRADG
                     GIVISASHNPHHDNGIKFFSAQGEKLDDATELAIEAALDAPFRTVASERLGRAVRTRD
                     TIGRYVEACKNSVPKGFNLGGMRIVVDCANGATYQLAPLVLRELGARLEAIGVDPNGL
                     NINAGVGSTHPEQLAARVLETGADLGIAFDGDGDRVLFVDAQGNIRDGDDLLYVLARD
                     WQGSGRLRGPVVGTLMTNYGFEQAMAALGIGFVRAKVGDRHVHQQLLANDGVLGGEAS
                     GHLLCLDRASTGDGIVSALQVLEVLQRRGIGLAQALDGLHKVPQKTVNVRYQGSSKPA
                     EAASVQAALAEALRAVEGRGRAFLRPSGTEPVVRVTVEADDAALVDATLATLADAVRA
                     AAA"
     gene            complement(2494..3381)
                     /gene="accD"
                     /locus_tag="PK399_03465"
     CDS             complement(2494..3381)
                     /gene="accD"
                     /locus_tag="PK399_03465"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPW12212.1"
                     /translation="MSWLKKLMPASIRTETGVDRKRSVPEGLWEKCERCGAVLYRPEL
                     EENLEVCPKCNFHMPIRARARLAALLDEGMGREIGAALGPTDVLKFKDQKKYSDRIKA
                     AQKSTGERDALIAMEGKLKGHDLVAAAFDFAYMGGSMGSVVGERFTLAAERALELRCP
                     FVNFSASGGARMQESLFSLMQMAKTSAALGRLRAAGLPYISVLTHPTTGGVSASFAML
                     GDLNLAEPEALIGFAGQRVIEQTVREKLPEGFQRSEFLLAHGTVDQIVDRREMRDRLA
                     HLLAMLMKQPAPADDVEAA"
     gene            complement(3456..4274)
                     /gene="trpA"
                     /locus_tag="PK399_03470"
     CDS             complement(3456..4274)
                     /gene="trpA"
                     /locus_tag="PK399_03470"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPW12213.1"
                     /translation="MTRLQERLDALRASGRKALVPFISAGDPSLEATVPVMHALAAAG
                     ADVIELGVPFSDPMADGPVIQRSSERALGRGAGLAFVLQAVRQFRAADATTPVVLMGY
                     LNPVEIRGAAGFAAAAAEAGVDGVLLVDLPPEEADEYRAAFSRQGLTLISLASPTTPE
                     TRLQRLCTCSEGYLYYVTFAGVTGADGLDVVEAGQRLRQLRAMASLPVYAGFGIRDAA
                     SAAGMAVHADGVVVGSALVSVLAQSSDANDAAARAGTFLRPLRQALDDLAAVPA"
     gene            complement(4271..4723)
                     /locus_tag="PK399_03475"
     CDS             complement(4271..4723)
                     /locus_tag="PK399_03475"
                     /inference="COORDINATES: protein motif:HMM:NF022786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HPW12214.1"
                     /translation="MADEHQNQAAADASAQAQNGPDPKFFDCVNEYLELANKQSQQYG
                     EKRISLASMYAAARFNAHVYLLNVKPIHAASERAHFLDYMTTMYRRMLNEHLDGMGQE
                     RGIDVGESELAAEYAAAGVQVGINPVQTPAGSYVAAAGAVPPAAPANE"
     gene            complement(4842..6080)
                     /gene="trpB"
                     /locus_tag="PK399_03480"
     CDS             complement(4842..6080)
                     /gene="trpB"
                     /locus_tag="PK399_03480"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPW12215.1"
                     /translation="MPSPITETAPGAAIDYSRYPDASGHFGRHGGRFVAETLIGPLEE
                     LAAAYDAARVDPAFIAAFEKDLKHYVGRPSPIYHAERLSRENGGAQILLKREDLNHTG
                     AHKINNTIGQALLASRMGKTRIIAETGAGQHGVASATVATRLGLECVVYMGATDIERQ
                     KINVYRMKLLGATVVPVTSGSATLKDALNEAMRDWVTNVQDTFYIIGTVAGPDPYPRM
                     VRDFNAVVGREARAQMLSEYGQLPDAITACVGGGSNAIGLFHAFLNDGGVRIVGAEAA
                     GDGIETGRHASSLSAGRIGVLHGNRTYVICDDDGQIIETHSISAGLDYPGVGPEHAFL
                     KDSGRAEYVGVTDDEALAAFHRLTRTEGILPALESSHAIAQALKLARELPKDALVLCN
                     LSGRGDKDVHTIAGHEGLNL"
     gene            complement(6269..6967)
                     /locus_tag="PK399_03485"
     CDS             complement(6269..6967)
                     /locus_tag="PK399_03485"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPW12216.1"
                     /translation="MKRALFRTRIKFCGLTRAGDVRLAGELGVDAIGLIFAPGSPRRI
                     EPEAARALREALAPLVDAVALFRDNSAGEVRDAIALARPTLLQFHGSEDDAFCRRFGL
                     PYLKGIGLKGVDAGLNGRMLHARYPHAAGFVLDGHAPGEPGGSGERVDIAAIPGDMSK
                     PYVLAGGLSADNVYAAVRAASPWGVDVASGIELEPGIKDGALMRRFVEEVRRADCIEL
                     GENGVIGECEACGR"
     gene            complement(6978..7778)
                     /gene="truA"
                     /locus_tag="PK399_03490"
     CDS             complement(6978..7778)
                     /gene="truA"
                     /locus_tag="PK399_03490"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPW12217.1"
                     /translation="MRFALGVEYDGSGFSGWQRLSPAGTHVADGSLQTALEIALSKVA
                     DARIDTVCAGRTDAGVHARCQVVHFDTDAVRDVRGWVLGTTANLPPAIAVRWCVPVAD
                     AFSARFAARSRRYRYRILNRGTRPGLARQYLSWERMPLDADAMHAAAQALRGERDFSA
                     FRSIQCQAAHARRDLHDIVVSRHDDEVHVEVQANAFLHHMVRNIVGSLLPVGRGERPV
                     EWIAGLLEGRDRSVAGPTAPAAGLLFLGPRYPRQWGLPPEVCGDDIPS"
     gene            complement(7796..9862)
                     /locus_tag="PK399_03495"
     CDS             complement(7796..9862)
                     /locus_tag="PK399_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HPW12218.1"
                     /translation="MIRTLRTAAIATALLLAAGNAWALGLGQIEVKSRRNQPLVAEIP
                     IISTTPGELEALQARLASPETFRRVGLAPPSGAAADLQFSLGSDARGRPVIRVTSTRP
                     VEQPVLNFLVEVDWGQGRLVREYSALVDAPNTASAPLQPAIEAPVSPVPNVIQRPVPP
                     PVAAAPVPTPPPVPEARPEPAPSPVAIAPLPPPAAAPAPIAAAPAPATAQYGPIKQGE
                     TLSKIAGKLDLPSGYSLDQTMLALLRANPDAFLGDDINRLRRGSVLRVPGRDELGSVS
                     ADEAGQVVRQQMQQWRQARRPVRQPEVATADAPPATRAPATAAPAAAAAPKATATTAK
                     PKPATPPRQAAPARRQEARLQIIPPAAPGKATGTRSGTTAGGEGSMLQQQLRQKDEDI
                     AAKAVEIGELKERVAELEKLKQDQQQLLSLKDSELAAAQQRLAEANKAAAAAAAAAVK
                     PAAAAQAAPQASAQVPATQDAAAAASNPMPYIWGGLGLAAVALLGWLLFGRRKPAPAP
                     RRSSFDSEALAASLVHPSADFDAPRVEDGDRHDADAQADSDTDTETDVDGSGPETPPP
                     VASAVADAPHAAPTIDLADVPPSAARVETPAWHSGWVKAEAFVPPPPPVEQAAAPRFV
                     PVEDDLPEPEIPVPQASAEQRMKLARAFLDIGDDHSAKQLLRELLDDADPVARTDAAR
                     LLRELG"
     gene            complement(9904..10956)
                     /locus_tag="PK399_03500"
     CDS             complement(9904..10956)
                     /locus_tag="PK399_03500"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008577526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPW12219.1"
                     /translation="MIRAAFPGHETNMSKQFKVAVVGATGAVGEVMLSILAERDFPAS
                     DVIALASERSAGMTVAFGNGDLVVQDLATFDPAGVDIALFSAGGATSKEYAPKFAAAG
                     AVVIDNSSTFRYDDDVPLVVSEVNPEAIRNRPRGIIANPNCSTMQLMVALKPIFDAAG
                     IERINVATYQSVSGAGRSGLEELGVQTGKLLNFQEIEPKKFQAQIAFNLIPHIDDFLD
                     NGYTKEEMKLVWETRKILGDDTIQVNPTAVRVPVFYGHSEAVNVETREKLGADRAREL
                     LEAAPGVVVVDERKAGGYPTPVTHASGNDAVYVGRIREDLSHPRGLNLWVVSDNIRKG
                     AALNAVQIAELVAAEA"
     gene            complement(11041..12156)
                     /gene="aroC"
                     /locus_tag="PK399_03505"
     CDS             complement(11041..12156)
                     /gene="aroC"
                     /locus_tag="PK399_03505"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPW12220.1"
                     /translation="MNTFGQLLRVTTFGESHGAAIGCVVDGCPPGIAIAPGDFVRDLA
                     RRATGTSRHTSQRHEADEVEILSGVYEGVTTGTPVALLIRNTDQRSKDYSKIAAQFRP
                     GHADYTYWQKYGIRDPRGGGRSSARETTMRVAAAVIAKKWLADRHGVRVYGWLSQLGE
                     IVPQGFDASVIETNPFFWPHAAQVDALETYMDALRKSGDSVGARVTVVADGVPPGWGE
                     PVYGKLDGDLAAAMMSINAVKGVEIGDGFASVSQKGSVHRDVMTPGGFLSNHAGGILG
                     GISTGQQVSVSVAFKPTSSLRLPVEGLDADGNVVDIVTTGRHDPCVGIRATPICEAML
                     ALVLMDQALRHRAQCGDVGEVSPRIPGATSINTDAKR"
     gene            complement(12167..13117)
                     /gene="prmB"
                     /locus_tag="PK399_03510"
     CDS             complement(12167..13117)
                     /gene="prmB"
                     /locus_tag="PK399_03510"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPW12221.1"
                     /translation="MSQELQTIIDLIRYGASRFNAAGLTFGHSYDNALDEATQLTLHA
                     LHLPHDLPPAYGNARITEAEKEDVLGLFLRRIEERIPAAYLTGEAWFAGLSFKSDTRA
                     LVPRSPIAEMIEAGFQPWLGHREVRRALDLCTGSGCIAIAMAHHNPDWHVDGADISDD
                     ALSLSAENEKRLHVRNVRFVKSDLFDGLQGEHYDLIVTNPPYVTNDETDALPREYAHE
                     PELGLRAGDDGLDLALRILRDAPLHLSQDGLLVCEVGEAETALVNLLPELPLAWVEFK
                     VGQMGIFVAECADLIAHHDRIAKLANARDASRAASSAGLF"
     gene            13200..13838
                     /locus_tag="PK399_03515"
     CDS             13200..13838
                     /locus_tag="PK399_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HPW12222.1"
                     /translation="MSKRAALLLLVACLAGGLGLWASQRFHAPASPALPAVKAVTLLP
                     DPRVLPAFSLQQSDGSPLTVDDLKGHWTIVFLGFTHCPDICPTTLAQLAGAQKQWAAL
                     PAATRPRVLFISTDPERDSPKLTGDYAHAFHADTLAATAPIPQLEEFARSLSLVFMKA
                     PGPSGEPDDYSIDHSAALVLLDPQVRMAGVAQPPFDIRAIAADLERLTKATP"
     gene            13835..14692
                     /gene="asd"
                     /locus_tag="PK399_03520"
     CDS             13835..14692
                     /gene="asd"
                     /locus_tag="PK399_03520"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HPW12223.1"
                     /translation="MSPLTALTYVLPHRALSSLARALAYSTNVSTKQWLIDTVTRKFG
                     VDLSEAAESDPTAYPTFNAFFTRALKPGARVPDPDPRTLLMPADGRISQCGDIVPDGS
                     GDGRIFQAKGQSFTAAELLGDAEAARPFADGVYATVYLSPRDYHRVHMPWTGTLRETV
                     HVPGRLFSVGTDAVASVPRLFARNERLVCHFDTDFGPMASVMVGALLVSGVETVWSGV
                     EIPAYGDRITRKDYRGKGIVLERFAEMARFNYGSTVIVLLPKGVARLAPELRAETPVR
                     LGQELGRLS"
     gene            14756..15499
                     /locus_tag="PK399_03525"
     CDS             14756..15499
                     /locus_tag="PK399_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12224.1"
                     /translation="MTASRAFAPDHPWDRNAHLAFVLIAWGATILGFGSSLVERLAGH
                     ADYPAPLVLHAHAALFIGWLGLLTIQVLLVRTNRTSVHRKLGLATLVLVPAMLAFSFL
                     AERHSQHFYSPKYPENLNFFAVPLGYLLVFPAFIGLALLARRNPPAHKRWMLLANAVI
                     LGVPFARWWGEALYERFGDGFWGMIVHTYAGTDLLVLLMVAYDIITRRRTHPALLAGV
                     PVLLASQLLVSWAYHSAWWPPIVRRIVGL"
     gene            complement(15519..17054)
                     /locus_tag="PK399_03530"
     CDS             complement(15519..17054)
                     /locus_tag="PK399_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPW12225.1"
                     /translation="MPVRPLPALAAFAVVLLAATSAPVAQAQSKRDLAAAATLQQRMD
                     AAEKRYRDALLAEDEDTDGAVPAAALTEMQAVVAACAKQKGCELPKMLATYERLLKAT
                     PQADDGDPDSEDEDPIDEGDLATGNVPGSADASALLSDEGQRFVRMVQFNPAVQAGIR
                     RWLTDMRPSLMDSYENYRYMQHLMSPAFKRHGLPEALLFGIMAKESNGKVHAGSRAGA
                     VGPLQFMPATGRRFGLGNDGTGFDTRYDPAASADAAAQYLTERMAQLDNNIELSLAGY
                     NGGEGRALRVYRESGGRSFWDVGVYNQFPAETKDYVPMVVAAAWLFLHPKDYGIRWPK
                     VNSRPAVIRLQQPASIYELTICLGNYGSRDGYMRALRNLNPRWDADSSMPAGTTLNAT
                     TRIASLYSRYCTRGQRLQLAEQLVASNVQSALVRIGPMTSTGGDAVANADAASATPVP
                     SKPKPQPPKPKTHKVKRGETLSSIVRKFDCDLGDLARANKLKAPKYAIKPGQSLRLEG
                     CKG"
     gene            17223..>18307
                     /locus_tag="PK399_03535"
     CDS             17223..>18307
                     /locus_tag="PK399_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPW12226.1"
                     /translation="MDPILVGKAVTTETSGNVFLLPKYGNRHGLIAGATGTGKTVTLM
                     TLAEGFSRIGVPVFLADVKGDVAGLAVAGTASEKLQQRIDLMGISDYRNEAAPVVFWD
                     LWGKLGHPVRTTVSEIGPTLLSRILELNETQAGVLDIVFKLADDRGLLLLDLDDLRAL
                     LGLIADERKDISTSYGLVSTPSIGAIQRALLRLEQEGADMFFGEPALELSDLMRTTTD
                     GRGVVNILAANQLILKPKLYSSFLLWLLSELFENLPEVGDLDKPKLVFVFDEAHLLFD
                     DAPAALRQRIEQVVRLIRSKGVGVYFCSQFPDDVPDEILGQMGNRFQHALRAFTPRDQ
                     KAVKTAAETFVANPALDVAATISQLGTG"
     assembly_gap    18308..18407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18408..18695
                     /locus_tag="PK399_03540"
     CDS             <18408..18695
                     /locus_tag="PK399_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPW12227.1"
                     /translation="MKYDNRIDRDSAAELLAKKAEAKVAQAEAPPAKTRDQDDAEQGG
                     FGGAVKDAVFGTKRRQGMIETMAKQTTRTIGAKLGNQIVRGILGSIFGGKR"
     gene            18763..19254
                     /locus_tag="PK399_03545"
     CDS             18763..19254
                     /locus_tag="PK399_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HPW12228.1"
                     /translation="MNRTLLAIALAATLAATAGCKPDEAAEAAAAPAVAAAPVNAVTE
                     VVDQGSPAGDVKGFDIKAFAGTYGGTLPCADCPGIDSTIAFTPEGTYTLSETYQDTDK
                     SSFLSKGTWSLRGDGKAVLLDPEDKDEYDRSFSIVSPTELRALDQQGKPIDSTLDYSL
                     KRK"
     gene            19257..19760
                     /gene="greB"
                     /locus_tag="PK399_03550"
     CDS             19257..19760
                     /gene="greB"
                     /locus_tag="PK399_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HPW12229.1"
                     /translation="MGRWRPPAAGSTALVTREGHDRLKAELDDLWRVRRPEVVKALAA
                     AAAEGDRSENAEYTYRKKQLGEIDRRVRYLSKRLEALRVVDAAPSDRDSVFFGAEVTL
                     ENIDDGQQVAYRIVGPDETDARRGWISIDAPLARAMLKKRVDDEFDAVLPGGIVHYAI
                     IGIRYAD"
     gene            complement(19748..20191)
                     /locus_tag="PK399_03555"
     CDS             complement(19748..20191)
                     /locus_tag="PK399_03555"
                     /inference="COORDINATES: protein motif:HMM:NF016045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HPW12230.1"
                     /translation="MAHQRWFTRLANAASRFAGSPSSFVLALLVVLVWIITGPLFGFS
                     DTWQLVINTGTTIVTFLMVFLIQSSQNRDARALQIKLDELIRATRGAHNSLLDLEELE
                     EHELRMFQDRYEALARRARERGVADDDDDVGTPDVDGGPTRDQSA"
     gene            complement(20272..21603)
                     /gene="rimO"
                     /locus_tag="PK399_03560"
     CDS             complement(20272..21603)
                     /gene="rimO"
                     /locus_tag="PK399_03560"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPW12231.1"
                     /translation="MSLQQPKVGFVSLGCPKALVDSERILTQLRVEGYDLVQAYDDAD
                     VVVVNTCGFIDSAVAESLDAIGEAMAENGKVIVTGCLGKRAEVIREAHPGVLSISGPQ
                     DYASVMDAVHTALPPRHDPFVNLLPDYGLKLTPKHYAYLKISEGCNHRCSFCIIPSMR
                     GDLVSRPVDEVLREAEKLVRGGVRELLVVSQDTSAYGVDVKYAERTWRDKTYQTRMKA
                     LCEGLSELDAWTRLHYVYPYPHVDDIIPLMADGKLLPYLDIPFQHASPRVLKLMKRPG
                     AVDKTLERVQRWRATCPELTIRSTFIVGFPGETEAEFEELLDFLDEAQLDRVGAFAYS
                     PVDGAKANELPDPVDEAVKQERLALFMERQAAISEARLAAKVGTVQQVLVDAIDGELA
                     IARSMADAPEIDGLVQVQDGREAGLVPGEFARVRIMGSDEHDLYGEVEYDD"
     gene            21699..22367
                     /locus_tag="PK399_03565"
     CDS             21699..22367
                     /locus_tag="PK399_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009590316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPW12232.1"
                     /translation="MGEWIHLDTAAGPARAWLARPAKPPRGSVIVLQEIFGVNAHIRA
                     VTDGFAGHGVVALAPALFDPVQPDVELGYGDAGVSRGRELVSELGTDGALQVVTAAAR
                     WLREAGHHPGVVGYCWGGSIAFLANTRLGLPAVSYYGARTMPFIAEPARAALMFHFGG
                     RDASIPADDIARHRALQPRAEAFVYPEAGHAFNRDIDPIHFHAASAALARRRTLAFLA
                     EHTS"
     gene            22364..22774
                     /locus_tag="PK399_03570"
     CDS             22364..22774
                     /locus_tag="PK399_03570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPW12233.1"
                     /translation="MNHWHLHPQLADDTTPVIELALCEVRLMDDANHPWLVLVPRVPD
                     LVEIIDLDATQRAQLTTEIDVASRALKALFKPHKLNIAALGNLVPQLHVHVIARYTDD
                     IAWPRPVWGAANARPYAPEELIERVTALRAALAG"
     gene            complement(22778..23350)
                     /gene="dcd"
                     /locus_tag="PK399_03575"
     CDS             complement(22778..23350)
                     /gene="dcd"
                     /locus_tag="PK399_03575"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005912996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HPW12234.1"
                     /translation="MSIKSDRWIRRMAREHGMLEPFEPGQVKQVDGHRIVSYGTSSYG
                     YDVRCSREFKIFTNINSTIVDPKHFDPKSFVDVVADECIIPPNSFALARTVEYFRIPR
                     DTLVVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPARIYANEGVAQMLFF
                     QADGDDVCETSYKDRGGKYQGQTGVTLPKT"
     gene            complement(23421..24269)
                     /gene="apbC"
                     /locus_tag="PK399_03580"
     CDS             complement(23421..24269)
                     /gene="apbC"
                     /locus_tag="PK399_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020852622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HPW12235.1"
                     /translation="MPRLTPHAVQGELKPLPHVRNIIAVGSGKGGVGKSTTAVNLALA
                     LAAEGLAVGVLDADVYGPSIPMMLGLSGRPDSPDGKTIEPIRAHGIEAMSIGLLVDQD
                     TPMIWRGPMATSSLTQLLEQTRWGDLDVLVVDLPPGTGDIQLTMAQKIPVAGAVIVTT
                     PQDIATLDARKALKMFEKLGIAVLGLVENMAVHVCTQCGHAEHLFGEGGAARMSAQYG
                     VPVLGSLPLEIGIREQGDAGMPMVAAQPDAAPSQAYRDIARAMLAQLDKRPKLRSGIM
                     ASLLGS"
     gene            24767..>26171
                     /locus_tag="PK399_03585"
     CDS             24767..>26171
                     /locus_tag="PK399_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009590311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPW12236.1"
                     /translation="MTLALAAALFLPVAHAQDQASQDQSAATADQKPKDLDKVTVTGS
                     LIPQTTLETFTPVTIISAEDIQARGFNNVAEVLQKSSFATGGVQNNQSSASFTQGAET
                     LSLFGLPSGYVKYLIDGRPMANYPALYNGSDVFNNISGIPVELVERIEILPGGQSSLY
                     GSDAIAGVVNIILKKNIDGGVVTARLGGYDEGGGRSGRISLADSFSSKDGRWNTLVGI
                     QAEKNDPVWGYQRDLTRQFNTDAYNGAAPLASRDWLVYSPFTSYKFLDPSNCANVSAG
                     FDGSVGLQTRPGFGDEKYCGSMTTPGYRTLKNSKDGIQLYTHTTFDFNDLAQGYADVL
                     LSREKVRYHIGSGYTWWGTSVGWGYFYDPRVGTGVADAYGELICGDDPDCLASLPPGD
                     LLNLQRAFTPEDMGGWENTMDENKSSSYSVTLGVNGTLSEDSNWDYDVSLTRTHYKLN
                     EISLARLADPINNYFQQH"
BASE COUNT         4021 a         8988 c         8810 g         4252 t
ORIGIN      
        1 actgcggcaa cgtgctggcc gacgaacgga tggcagcggt gttcttctgg atgggcgcgt
       61 aagccccgtt tcttacgtct agccagagaa gaaggccgct tgcgcggcct tcgttcactt
      121 cagcttgatc tcgcgcaggc gctcttccag gtagccctgt gcggtgatcg gttccggata
      181 gcggctgggg ttgtccgggg taatcgtgga cggcagcacg tcgatcagga aatccggatt
      241 ggggtgcagg aagaaaggca ccgaataacg cggctggcgc gccgcctcgc ccgggggatt
      301 ggtcacccgg tgggtggtcg acggatacac gtggttggtc agccgctgca gcatgtcgcc
      361 gatgttgacc acgatggtgt cggcgtccga ggtgaagggc acccactcgc ccttcttcga
      421 cagcacttcc aggccggccg cgctcgcgcc gaccagcagg gtgatgaggt tgatgtcctc
      481 gtgcgcaccg gcgcgcacgt tggggatgtc gtcggtggtg atcggtggat agtggatcgg
      541 acgcaggatc gagttgccgc tgtcggtctt gtcggcgaag tagtcctccg gcaggccgat
      601 gtgcagggcc agcgccgaca gcacctgcga gcccagcgca tccagcgact cgtacaggcc
      661 atagccgacg cgctggaagc cgggcacttc gtccggccac aggttcggcg ccatgtcggc
      721 ggcatatttc gaatcgcgcg ggatctcgcg gccgatgtgc cagaactcct tgaggtcgga
      781 gtatttcgca cccttcgcgg tttccacgcc gaacgcggta tagccgcgcg cgccgccgcc
      841 gccgggcacg tgatacttct tcttggtctc ttccggcaag gcgaagaacg cgcggaaggt
      901 ggcgtaggcc tcgtcgatct gcgcctgcgg gatgccgtgg ttgcggatgc cggcaaagcc
      961 ccattcgcga taggccgcgc cgagctcatc gatgaaagcc tggcgatccg cgggcgatgc
     1021 cggatgggtg aagcggcgga tgtcgagggt cgggatgcgt gcggccatgt cggttgctcc
     1081 ggtgatcggc ggcggcgatt gcatcacgcc gctcgcgaag gattcttgat cgaaatcagg
     1141 cggcggccgc gcgcactgcg tcggccagcg ttgccagggt ggcatcgacc agcgcggcat
     1201 cgtcggcttc gaccgtcacc cgcaccaccg gctcggtgcc ggacgggcgc aggaacgcac
     1261 gcccgcggcc ttcgaccgcg cgcagggcct cggcgagcgc ggcctgcacg cttgcggcct
     1321 cggccggttt gctgctgccc tggtagcgca cgttgacggt cttctgcggg accttgtgca
     1381 gaccgtccag cgcctgcgcc aggccgatgc cacgacgttg caggacttcc agcacctgca
     1441 gcgcactgac gatgccgtcg ccggtgctgg cgcggtccag gcacagcagg tggccgctgg
     1501 cctcgccacc cagcacgccg tcgttggcca gcaattgctg gtggacatgg cggtcgccga
     1561 ccttggcgcg gacgaagccg atgcccaggg ccgccatcgc ctgttcgaaa ccgtagttgg
     1621 tcatcagggt gccgaccacc gggccacgca gtcgcccact gccttgccag tcgcgggcca
     1681 gcacgtacaa caggtcgtcg ccatcgcgga tgttgccctg cgcatcgacg aacagcaccc
     1741 ggtcgccatc gccatcgaag gcaatgccca ggtcggcgcc ggtctcgagc acgcgcgcgg
     1801 ccagctgttc cggatgggtc gaaccgacgc cggcgttgat gttgaggccg ttcgggtcga
     1861 ccccgatcgc ctccaggcgc gcgccgagct cgcgcaacac cagcggcgcc aactggtagg
     1921 tggcgccgtt cgcgcaatcg accacgatgc gcatgccgcc caggttgaag cccttcggca
     1981 ccgaattctt gcaggcctcg acgtagcggc cgatggtgtc gcgggtgcgc actgcgcggc
     2041 ccagtcgctc cgaggccacg gtgcggaacg gcgcgtccag cgcggcctcg atcgcgagct
     2101 cggtggcgtc gtcgagcttc tcgccctgcg cggagaagaa cttgatgccg ttgtcgtggt
     2161 gcgggttgtg cgaagcggaa atcacgatgc cgccatcggc gcgcatcgaa tgggtgaggt
     2221 gggcgacggc gggggtcggc atcggcccca tcagctgcac gtccacgccg gcggcgacca
     2281 ggccggcttc cagcgcggcc tcgaacatgt agttggagat gcgggtgtcc ttgccgatca
     2341 ccaccatcgg gttgcgccat tcgcgatcgg catcgcgggc ggcgaccagc gcgtggccgt
     2401 acgcgttgcc caggcgcagc acgaaatcgg cggagatcgc cccctcgccg acgcggccgc
     2461 ggataccgtc cgtgccgaag tacctgcgtg cgctcatgcg gcctcgacgt cgtcggcagg
     2521 ggccggctgc ttcatcagca tggcgagcag gtgggccagg cgatcgcgca tctcgcggcg
     2581 gtcgacgatc tggtcgacgg tgccatgcgc cagcaggaat tcgctgcgct ggaagccctc
     2641 cggcagcttt tcgcgcacgg tctgctcgat cacgcgctgg ccggcgaacc cgatcagcgc
     2701 ttccggttcg gcgaggttga ggtcgcccag catggcgaaa ctggcggaca cgccgcccgt
     2761 ggtcggatgg gtcagcacgg agatgtacgg caggcccgcc gcacgcagcc ggcccagcgc
     2821 agcagaggtc ttggccatct gcatcagcga gaacaggctc tcctgcatgc gcgcgccgcc
     2881 gctggcggag aagttcacga acggacagcg cagttccagc gcgcgttccg cggcgagggt
     2941 gaagcgctcg ccgaccaccg atcccatcga accgcccatg taggcgaaat cgaacgcggc
     3001 ggcgaccagg tcatggccct tcagcttgcc ttccatcgcg atcagcgcgt cgcgctcgcc
     3061 ggtggatttc tgcgcagcct tgatccggtc ggaatacttc ttctggtcct tgaacttgag
     3121 cacgtcggtg gggcccagcg cggctccgat ttcacggccc atgccctcgt ccagcaatgc
     3181 cgccagccgc gcgcgggcgc ggatcggcat gtggaaattg cacttcgggc agacctcgag
     3241 gttttcctcg agctccgggc gatacagcac cgcgccgcag cgctcgcatt tctcccagag
     3301 gccttccggc acgctgcgct tgcgatccac cccggtttcg gtgcggatgc tcgcgggcat
     3361 cagtttctta agccagctca ttcggttcgt tcaccgctgt tgtcgggtgc cgcgtcgccg
     3421 cgcgatccgc gcggatgccg gtgtcgcagt ctagctcagg ccgggacggc ggccaggtcg
     3481 tccagggcct ggcgcaacgg ccgcaggaac gtgcccgcgc gcgccgccgc gtcattcgcg
     3541 tcgctgctct gcgccagcac ggagaccagc gcgctgccca ccaccacgcc gtcggcatgc
     3601 accgccatcc cggctgcgct ggcggcatcg cggatgccga acccggcata cacgggcagc
     3661 gaggccatgg cccgcaactg gcgcaggcgt tggccggctt cgaccacgtc gaggccatcg
     3721 gcaccggtga caccggcgaa cgtgacgtag tacaggtagc cttccgaaca cgtgcacagg
     3781 cgctgcaggc gcgtttccgg ggtggtcggc gaggccagcg aaatcagcgt caggccctgg
     3841 cgggagaacg ccgcgcggta ttcgtcggct tcttccggcg gcaggtcaac cagcagcacg
     3901 ccatcgacgc cggcctccgc ggcggccgcg gcaaagccgg cggcaccgcg gatctcgacc
     3961 ggattcaggt agcccatcag caccaccggc gtggtcgcat ccgcggcacg gaattgacgc
     4021 accgcctgca gcacgaaggc cagcccggca ccgcgcccca gcgcgcgctc ggagctgcgc
     4081 tggatcaccg ggccatccgc catgggatcg gagaacggca cgcccagctc gatcacatcc
     4141 gcaccggccg cggccaatgc gtgcatgacc ggcaccgttg cctccagcga cggatcgccg
     4201 gcgctgatga acgggaccag agccttgcga ccggacgcgc gcaacgcgtc cagcctttcc
     4261 tgcaaccggg tcactcgttc gccggtgcgg ccggcggcac tgcgcccgcg gcagccacgt
     4321 agctcccggc cggcgtctgc accgggttga tgccgacctg cacgccggca gcggcgtact
     4381 cggctgccag ctcggactcg cccacgtcga tgccgcgctc ctggcccatg ccgtccaggt
     4441 gctcgttgag catccggcgg tacatggtgg tcatgtagtc caggaagtgg gcgcgctcgc
     4501 ttgcggcgtg gatcggcttc acgttgagca ggtagacgtg ggcattgaag cgcgcggcgg
     4561 cgtacatgct ggccaggctg atgcgcttct cgccgtactg ctgggactgc ttgttggcga
     4621 gttccaggta ttcgttgacg caatcgaaga acttcggatc cggcccgttc tgggcctgtg
     4681 cggaagcgtc ggccgcagcc tggttctggt gttcgtcggc catggccgtc atcctcgtgg
     4741 tgtgttttcg cagccggtta ttgtctcgca accggcgggc cgaggccagt tcaggcggcg
     4801 gcacccttcc cggcgccacc accggcacat cgcgccgggg atcagaggtt caagccctca
     4861 tgccccgcga tggtgtgtac gtccttgtcg ccgcgacccg acaggttgca caggaccagc
     4921 gcgtccttcg gcaactcccg cgcaagcttg agcgcctggg cgatcgcatg gctggattcc
     4981 agcgccggca ggatgccctc ggtccgggtg agcctgtgga aggcggccag cgcctcgtcg
     5041 tcggtgacgc cgacgtactc ggcgcggccg ctgtccttca ggaacgcgtg ctccggaccc
     5101 acgccgggat agtccagccc ggccgagatc gaatgggtct cgatgatctg gccgtcgtca
     5161 tcgcagatga cgtaggtgcg attgccgtgc agcacgccga tccggcccgc cgacagcgag
     5221 gaggcatggc ggccggtctc gatgccgtcg ccggccgcct cggcgccgac gatgcgcacg
     5281 ccgccgtcgt tgaggaacgc atggaacagg ccgatcgcat tgctgccgcc accgacgcag
     5341 gccgtgatcg catccggcag ctgcccgtat tcggacagca tctgcgcgcg tgcctcgcgg
     5401 ccgaccaccg cgttgaaatc gcgcaccatg cgcgggtacg ggtccgggcc ggccacggtc
     5461 ccgatgatgt agaaggtgtc ctgcacgttg gtgacccagt cgcgcatcgc ctcgttcagc
     5521 gcatccttca gggtggccga accactggtc accggcacca ccgtcgcccc gagcagcttc
     5581 atccggtaga cgttgatctt ctgccgctcg atgtcggtgg cgcccatgta gaccacgcat
     5641 tccaggccca gccgagtggc cacggtggcg ctggccacgc catgctggcc ggcgccggtc
     5701 tcggcgatga tccgggtctt gcccatccgc gacgccagca gggcctggcc gatcgtgttg
     5761 ttgatcttgt gcgcgccggt gtggttcagg tcttcgcgct tgagcaggat ctgcgcgccg
     5821 ccgttttcgc ggctcaggcg ctccgcgtgg tagatcgggc tgggccggcc gacgtagtgc
     5881 ttgaggtcct tctcgaacgc ggcgatgaaa gcgggatcga cgcgcgcggc gtcgtaggcg
     5941 gccgccagtt cttccagcgg gccgatcagg gtttccgcga cgaagcggcc gccgtggcga
     6001 ccgaaatggc cggaggcgtc gggatagcgg gagtagtcga tggccgcgcc gggcgcagtc
     6061 tcggtgattg gcgatggcat gcaggtgctc cggaagcggg cgcagccgat cgggctgcgg
     6121 aaaggcgggt cttcgctgcg gagaggggcg tcaccagacc atggcggccg gcacccggcg
     6181 ccagcgacgc agggaacggc cgaacggagc gtgtttgccg cgcggggtgg tcatcgggac
     6241 acagggtaca acagcgcggc gggagcgctc agcgaccaca ggcctcgcac tccccgatga
     6301 cgccgttctc gccaagttcg atgcaatcgg cacgccggac ttcctccacg aagcggcgca
     6361 tcaacgcgcc atccttgatc ccgggttcca gttcgatgcc gctggcgaca tccacgcccc
     6421 acggcgacgc cgcgcgcacc gcagcgtaca cattatcggc gctgagccca cccgccagca
     6481 cgtaaggctt gctcatgtcg ccgggaatcg cggcgatgtc gacgcgctcc ccgctgccgc
     6541 cgggctcgcc gggcgcgtgg ccgtccagca cgaatcccgc ggcgtgcggg tagcgcgcat
     6601 gcagcatgcg gccgttgagg ccggcatcca cgcccttcag gccgatgccc ttgaggtaag
     6661 gcaggccgaa gcgacggcag aacgcatcgt cctcgctgcc atggaactgc agcagggtgg
     6721 gtcgcgccag ggcgatcgcg tctcgcacct caccggcgct gttgtcccgg aacagcgcca
     6781 ccgcatcgac cagcggcgcc agcgcctcgc gcagggcgcg ggccgcttcg ggttcgatcc
     6841 ggcgcgggct gccaggggcg aagatcaggc cgatcgcatc gacgcccaac tcgccggcca
     6901 ggcgcacgtc gccggcacgg gtcaggccgc agaacttgat gcgggtacgg aacaacgcac
     6961 gcttcatcgg cgaaccttca gctggggatg tcgtcgccgc agacctccgg cggcagcccc
     7021 cattgtcgcg gatatcgcgg cccgaggaac agcagcccgg cggccggtgc ggtcggcccg
     7081 gcgacactgc gatcacggcc ttccagaagt ccggcgatcc attcgacagg acgctcgcca
     7141 cggccgaccg gcagcagcga acccacgatg ttgcggacca tgtggtggag gaaggcattc
     7201 gcctgcacct ccacgtggac ctcgtcgtcg tggcgcgaca ccacgatgtc gtgcaggtcg
     7261 cgccgcgcat gcgcagcctg gcactggatg ctgcggaacg cggagaaatc gcgctccccg
     7321 cgcaaggcct gcgcggcggc gtgcatggca tcggcatcca gcggcatgcg ctcccagctg
     7381 aggtactggc gggcgagccc gggccgcgtg ccgcggttga ggatgcgata gcggtaacgg
     7441 cgcgagcgcg cggcgaaacg cgcactgaac gcgtcggcga ccggcacgca ccagcgcacg
     7501 gcgatggcgg gtggcaggtt ggcggtggtg ccgagcaccc aaccccgtac atcgcggacg
     7561 gcgtcggtgt cgaaatgcac gacctggcag cgcgcgtgca cgccggcatc ggtgcggccc
     7621 gcgcagacgg tatcgatgcg cgcatcggcc actttcgaca acgcgatctc cagtgcggtc
     7681 tgcaggctcc catcggcgac atgggtgccg gccgggctca gccgttgcca gccggagaac
     7741 ccgctgccgt cgtattcgac ccccagcgcg aaacgcatcc gcgtgccgac gtgcctcagc
     7801 cgagctcgcg cagcaggcgg gcggcgtcgg tgcgcgcgac cgggtcggcg tcatccagca
     7861 gttcgcgcag cagctgcttc gcggaatggt cgtcgccgat gtccaggaag gcacgcgcca
     7921 gcttcatccg ctgctcggcg ctggcctgcg gcaccgggat ctccggctcc ggcaaatcgt
     7981 cctcgaccgg cacgaagcgt ggcgcagcgg cctgctcgac cggtggcggc ggcggcacga
     8041 aggcctcggc cttgacccag ccggaatgcc aggccggggt ttccacccgc gcggcgctgg
     8101 gcgggacgtc ggccaggtcg atggtgggtg cggcgtgcgg cgcgtccgca accgcgctgg
     8161 cgaccggcgg cggcgtttcc ggtccgctgc catcgacgtc ggtctcggtg tcggtgtcgg
     8221 agtcggcttg agcgtcagca tcgtggcgat cgccatcctc gacgcgcggg gcgtcgaagt
     8281 cggccgaggg atgcaccagg ctggcggcaa gcgcttcgct gtcgaaactg ctgcggcgcg
     8341 gcgcgggagc cggcttgcgg cgcccgaaca gcaaccatcc cagcaaggcc accgcagcca
     8401 agcccagccc gccccagatg tacggcatcg ggttggaggc agcagcggct gcgtcctggg
     8461 tcgcgggcac ttgcgcgctg gcctgcggcg cggcctgtgc ggcagcggcc ggcttgaccg
     8521 cggccgccgc tgccgccgcg gcggccttgt tcgcctcggc caggcgctgc tgggcggcag
     8581 ccagttcgct gtccttgagc gacagcaact gctgctggtc ctgcttcagt ttctcgagtt
     8641 cggccacccg ctccttgagc tcaccgatct cgacggcctt ggcggcgatg tcttcgtctt
     8701 tctgccgcaa ttgttgctgg agcatgctgc cctcgcctcc ggcggtggtg ccggatcgtg
     8761 tacctgtggc cttgcccggt gcagcgggcg ggatgatctg caagcgcgct tcctggcgac
     8821 gcgcgggcgc ggcctggcgg ggcggcgttg ccggtttcgg cttggccgtg gtggcggtcg
     8881 ccttgggcgc cgcagctgcg gcgggggcag ccgtggccgg cgcccgcgtg gccggagggg
     8941 catcggcagt ggcgacctcg ggctggcgca ccggacggcg cgcctggcgc cattgctgca
     9001 tctgctggcg caccacctgc ccggcttcgt ctgcgctgac gctgcccagt tcatcgcgtc
     9061 cggggacgcg caacaccgaa ccgcgacgca gccggttgat gtcatcgccc aggaaggcat
     9121 ccggattggc gcgcagcaag gccagcatcg tctggtcaag gctgtagccc gacggcaggt
     9181 ccagcttgcc ggcgattttc gacagcgtct cgccctgctt gatcggaccg tattgcgcgg
     9241 tggcaggtgc tggcgctgcg gcgatcggag ccggtgcggc agccggtggt ggcagcggcg
     9301 cgatggcgac gggcgatggc gcgggttccg ggcgcgcctc gggaaccggc ggcggcgtgg
     9361 gcacgggcgc ggcggcaacc ggtggcggca ccggccgctg gatcacgttc ggcaccggcg
     9421 acaccggggc ttcgatggcc ggctgcagcg gcgcgcttgc ggtattcggc gcgtcgacca
     9481 gcgccgagta ttcgcgcacc agccgaccct gtccccaatc gacctcgacc aggaagttca
     9541 gcacgggctg ttcgaccggg cgggtgctgg taacccggat cacaggacgg ccgcgggcat
     9601 cgctgcccag gctgaactgc aggtctgcgg cggcgccgct gggcggcgcc aggccgaccc
     9661 gccggaaggt ttccggcgag gccagccgtg cctgcaacgc ttccagttcc cccggcgtgg
     9721 tcgaaatgat cgggatttcc gcgaccagcg gctggttgcg ccgcgacttg acctcgatct
     9781 ggccgaggcc gagcgcccac gcattccccg ctgccaacag caacgcagtg gcgattgccg
     9841 ccgttcgcaa tgtcctgatc acgcctgctc ccgcccccat caccgcgacg actctagccg
     9901 cgctcaggct tcggcggcaa caagttccgc aatctgcacc gcgttcagcg ccgcgccctt
     9961 gcggatgttg tccgagacca cccacaggtt cagcccgcgc ggatgcgaca ggtcctcgcg
    10021 gatccggccc acgtagaccg cgtcgttgcc cgaggcatgg gtgaccggcg tgggatagcc
    10081 gccggccttg cgctcgtcga ccaccaccac gcccggcgca gcctcgagca gttcgcgcgc
    10141 gcggtcggcg ccgagcttct cgcgcgtctc gacgttgacc gcttcggaat gcccgtagaa
    10201 caccggcacg cgcaccgcgg tcgggttgac ctggatggtg tcgtcgccaa ggatcttgcg
    10261 ggtctcccag accagcttca tctcttcctt ggtgtagccg ttgtccagga agtcgtcgat
    10321 gtgcgggatc aggttgaagg cgatctgcgc ctggaacttc ttcggttcga tctcctggaa
    10381 gttcagcagc ttgccggtct gcacgcccag ctcctccagc ccggagcgac ccgcgcccga
    10441 caccgactgg tacgtcgcca cgttgatgcg ctcgatgccg gcggcgtcga agatcggctt
    10501 cagcgccacc atcagttgca tggtcgagca gttcgggttg gcgatgatcc cgcgcgggcg
    10561 gttgcggatg gcttcgggat tgacctcgga caccaccagc gggacgtcgt cgtcgtagcg
    10621 gaaggtcgag gagttgtcga tcaccaccgc gccggcggcg gcgaacttcg gcgcgtattc
    10681 cttgctggtg gcaccgcccg cggagaacag cgcgatgtcc acgccggcgg gatcgaaggt
    10741 ggccaggtcc tgcaccacca ggtcgccgtt gccgaaggcc accgtcatgc cggcggaacg
    10801 ctcgctggcc agggcgatga cgtcgctggc cgggaagtcg cgctcggcca ggatcgacag
    10861 catcacttcg cccacggcgc cggtcgcgcc caccactgcc accttgaatt gcttgctcat
    10921 gttcgtctca tgccctggaa atgcggcgcg gatcatcgcg ctgcggaatt cggaatggat
    10981 tgccttgcgt gtccgctgcg gcgcgctgct gcccgtgggg gcgtagccgg cggcgcgccg
    11041 ctatcgtttc gcgtcggtgt tgatggacgt ggcgcctgga atgcgcggcg aaacctcgcc
    11101 cacgtcgccg cactgcgcgc gatggcgcag cgcctggtcc atcagcacca gcgccagcat
    11161 cgcctcgcag atcggggtcg cacggatgcc cacacaggga tcgtggcgac cggtggtgac
    11221 gatgtcgacc acgttgccgt cggcatccag tccttccacc ggcaggcgca ggctggaggt
    11281 cggcttgaac gccaccgaga ccgaaacctg ctggccggtg gaaatgccgc ccaggatccc
    11341 gcccgcatgg ttcgacagga acccgcccgg cgtcatcacg tcgcgatgca cgctgccctt
    11401 ctgcgaaaca ctggcgaagc cgtcgccgat ctccacgccc ttgaccgcat tgatcgacat
    11461 catcgccgcg gccaggtcgc catcgagctt gccgtacacc ggctcgcccc agcccggcgg
    11521 cacgccatcg gcgaccacgg tcacccgcgc gccgaccgag tcgccggact tgcgcagcgc
    11581 gtccatgtag gtttccagcg catcgacctg cgcggcgtgc ggccagaaga acggattggt
    11641 ctcgatgacg gacgcatcga acccctgcgg cacgatctcg ccgagttgcg acagccagcc
    11701 gtagacgcgc acgccatgcc ggtccgccag ccacttcttg gcgatcaccg ccgcggcgac
    11761 gcgcatggtg gtctcgcggg cgctgctgcg gccaccgcca cgtggatcgc ggatgccgta
    11821 tttctgccag taggtgtagt cggcgtggcc gggccggaac tgcgcggcga tcttgctgta
    11881 gtccttgctg cgctggtcgg tgttgcggat cagcagcgcg accggcgtgc cggtggtgac
    11941 gccttcgtag acgccgctga ggatttcgac ctcgtcggct tcatgccgtt gcgaggtgtg
    12001 ccgcgaggtg ccggtcgcac gccgcgccag gtcccgcacg aagtccccgg gcgcgatcgc
    12061 gatgcccggc gggcagccat cgaccacgca cccgatcgcc gccccgtggg actcgccgaa
    12121 ggtggtgacg cgcagcagct ggccgaaggt gttcaagcgg gcagagtcag aacagtccgg
    12181 cgctggatgc ggcccggctg gcatcgcgcg cattcgccag cttggcgatg cggtcgtggt
    12241 gggcgatcag gtcggcgcat tcggccacga agatgcccat ctgcccgacc ttgaattcga
    12301 cccaggccaa cgggagttcc ggcagcaggt tgaccagcgc ggtctcggcc tcgccgacct
    12361 cgcagaccag caggccgtcc tgcgaaagat gcagcggcgc gtcgcgcagg atgcgcagcg
    12421 cgaggtccag gccgtcgtcg ccggcccgca ggccgagttc cggctcgtgc gcgtattcgc
    12481 gcggcagggc gtcggtctcg tcgtttgtga cgtagggcgg attggtgacg atcaggtcgt
    12541 aatgctcgcc ctgcaggcca tcgaacaggt ccgacttgac gaagcggacg ttgcgcacgt
    12601 gcaggcgctt ttcgttctcg gcggacagcg acagcgcgtc gtcgctgatg tcggcgccgt
    12661 ccacgtgcca gtcggggttg tggtgcgcca tcgcgatggc gatgcaaccg gacccggtgc
    12721 acaggtccag ggcgcggcgc acctcgcggt ggcccagcca gggctggaag ccggcttcga
    12781 tcatttccgc gatcggcgaa cgcggcacca gcgcgcgcgt gtccgacttg aagctcaggc
    12841 cggcgaacca ggcctcgccg gtgaggtagg cggccgggat gcgctcctcg atacggcgca
    12901 ggaacaggcc cagcacgtct tccttctcgg cctcggtgat gcgggcattg ccgtaggccg
    12961 gcggcaggtc gtgcggcagg tgcagcgcat gcagggtcag ctgggtcgct tcgtccaggg
    13021 cgttgtcgta gctgtggccg aaggtcaggc ccgccgcgtt gaagcggctg gcgccgtagc
    13081 ggatcaggtc gatgatcgtc tgcaattcct gggacatgag cggccacgcg cgtgcaaggc
    13141 cgtgaagtat agcggcggtg ccccgctatc atggcggccc gctaccattc cggacggtca
    13201 tgtcgaaacg cgctgccctg ctgttgctcg tggcttgcct ggccggggga ttgggcctgt
    13261 gggcctcgca gcgcttccat gcgcccgcct ccccagcgct gcctgcggtg aaggcggtga
    13321 ccttgctgcc ggatccgcgc gtgctgcccg cgttctcgct gcagcaatcc gatggcagcc
    13381 cgctgaccgt cgacgacctc aagggccact ggaccatcgt gttcctgggc ttcacccatt
    13441 gccctgacat ctgcccgacc acgctggcgc agctcgctgg cgcacagaag cagtgggcgg
    13501 cgctaccggc cgccacccgc ccgcgcgtcc tgttcatttc caccgaccca gagcgcgaca
    13561 gccccaagct caccggcgac tacgcgcatg ccttccatgc ggacacgctg gcggcgaccg
    13621 cgccgatccc gcaactggag gaattcgcgc gctcgctgtc gctggtgttc atgaaggcgc
    13681 ccggccccag cggcgagccg gacgactaca gcatcgacca cagtgccgcc ctcgtcctgc
    13741 tcgacccgca ggtgcgcatg gccggcgtgg cgcaaccccc gttcgacatc cgcgcgatcg
    13801 cggccgacct cgaacgcctg accaaggcca cgccgtgagc ccgttgaccg ccctgaccta
    13861 cgtcctgccg catcgcgcgc tgtcctcgct ggcgcgcgcg ctggcttact cgaccaacgt
    13921 gtcgaccaag cagtggctga tcgataccgt cacccgcaag tttggcgtgg acctgtccga
    13981 agccgccgaa tccgacccca cggcctaccc caccttcaat gcgttcttca cccgcgcgct
    14041 gaagcccggt gcacgcgtgc cggatccaga cccgcgcacg ctgctgatgc ccgccgatgg
    14101 ccgcatcagc cagtgcggcg acatcgtgcc cgatggaagc ggtgacggac gcatcttcca
    14161 ggccaagggc cagtcgttca ccgccgccga attgctgggc gatgccgaag ccgccaggcc
    14221 cttcgccgat ggcgtgtacg ccaccgtgta cctgtcgccg cgcgactacc accgcgtgca
    14281 catgccgtgg accggcaccc tgcgcgagac cgtgcacgtg ccggggcgct tgttctcggt
    14341 cggcacggac gcagtggcca gcgtgccgcg gctgttcgcc cgcaacgagc gcctggtctg
    14401 ccacttcgac accgacttcg ggccgatggc ctcggtgatg gtgggcgcgc tgctggtgtc
    14461 cggcgtggaa accgtgtgga gcggcgtgga gatccccgcc tacggcgacc gcatcacccg
    14521 caaggactat cgcggcaagg gcatcgtgct ggaacgcttc gccgagatgg cgcgcttcaa
    14581 ctacggcagc acggtgatcg tactgttgcc gaagggcgtg gcgcggctgg caccggagtt
    14641 gcgtgcggaa acgccggtgc gcctgggaca ggagctcgga cgcctttcat aggggttccg
    14701 gcgctggccg atatcccggc ttacactgcg cggcaacggg accggggagt ccacgatgac
    14761 cgcaagcaga gcgttcgcgc ccgaccaccc gtgggatcgc aatgcccatc tggcattcgt
    14821 cctgattgcc tggggcgcca cgatcctggg attcggctcg tcgctcgtgg agcgcctggc
    14881 gggccatgcg gattaccccg cgccgctggt actgcacgcg cacgcggcac tgttcatcgg
    14941 ctggctgggt ttgttgacga tccaggtcct gctggtccgc acgaacagga catccgtcca
    15001 ccgcaagctc ggcctcgcga cgctcgtgct ggtgccggcc atgctggcct tcagtttcct
    15061 ggcggaacgc cacagccagc atttctacag cccgaaatac ccggaaaacc tcaatttctt
    15121 cgcggtgccg ctcggctacc tgctggtgtt ccccgccttc atcggcctgg cactgcttgc
    15181 ccgtcgcaat ccgccggcgc acaagcgctg gatgctgctt gccaacgccg tcatccttgg
    15241 cgtgccgttc gcgcggtggt ggggcgaggc actgtacgag cggttcggcg acggcttctg
    15301 gggcatgatc gtgcacacct atgccggcac ggatctgctc gtattgctga tggtggcgta
    15361 cgacatcatc acgcgccgcc gcacgcatcc cgcgctactt gcgggcgtcc ccgtgctgct
    15421 ggccagccaa ctcctcgtgt catgggccta tcacagcgcc tggtggccgc ccatcgtgcg
    15481 ccggatcgtc gggctttagc gccgctcgga ttccccgttc agcccttgca cccctccaac
    15541 ctcaggctct gtcccggctt gatcgcgtat ttcggcgcct tcagcttgtt cgccctggcc
    15601 aggtcgccga ggtcgcagtc gaacttgcgg acgatcgacg acagggtctc gccgcgcttg
    15661 accttgtggg tcttcggttt cggcggctgc ggcttgggct tggagggcac gggcgtggcg
    15721 ctggcggcat ccgcgttggc cacggcatcg ccgccggtgc tggtcatcgg cccgatccgc
    15781 accaacgccg attgcacgtt gctcgccacc agctgttccg caagctgcag gcgctggccg
    15841 cgcgtgcagt agcggctgta cagcgacgcg atgcgggtgg tggcattgag cgtggtgccg
    15901 gccggcatgc tgctgtcggc gtcccaacgc ggattgaggt tgcgcagcgc acgcatgtag
    15961 ccgtcgcgcg acccgtagtt gcccaggcag atggtgagtt cgtagatcga ggccggctgc
    16021 tgcaggcgga tcaccgccgg gcgcgagttc accttcggcc agcggatgcc gtagtccttc
    16081 ggatgcagga acagccaggc tgcggcgacc accatcggca cgtagtcctt ggtctcggcg
    16141 gggaactggt tgtagacgcc gacgtcccag aaactgcgac cgccgctctc gcggtacacg
    16201 cgcagggcgc gcccttcgcc gccgttgtaa ccggccagcg acagttcgat gttgttgtcg
    16261 agctgggcca tgcgctcggt caggtactgc gctgcggcat ccgcggatgc cgcggggtcg
    16321 taacgggtgt cgaaaccggt gccgtcgttg cccaggccga agcggcggcc ggtggccggc
    16381 atgaactgca gcggcccgac cgcgcccgcg cgcgaacccg catgcacctt gccgttggac
    16441 tccttggcca tgatcccgaa cagcagcgcc tccggcaggc cgtggcgctt gaatgccggc
    16501 gacatcaggt gctgcatgta gcggtagttc tcgtaactgt ccatcagcga cgggcgcatg
    16561 tcggtcagcc agcggcggat gccggcctgc accgccggat tgaactggac catgcgcacg
    16621 aagcgctggc cttcatcgct cagcagcgca gacgcatcgg cgcttcccgg cacgttgccc
    16681 gtggccaggt cgccctcgtc gatcggatcc tcgtcctcgc tgtccggatc gccgtcgtcc
    16741 gcttgcgggg tggccttgag caggcgctcg taggtggcca gcatcttcgg cagttcgcag
    16801 cccttttgct tcgcgcaggc cgcgaccacc gcctgcatct cggtcaaggc cgcggcgggg
    16861 acggcgccat cggtgtcctc gtcctcggcc agcagggcat cgcgatagcg cttctcggcg
    16921 gcatccatcc gctgctgcaa ggtcgccgcg gcggcaaggt cacgcttgga ttgcgcctgc
    16981 gcaaccggcg cggacgtggc ggcaagcagg acgacggcga aggcggcaag cgcgggcagt
    17041 gggcgaacgg gcatcagcaa tgggcaactc gcgtggggaa cggcagggta gccgccgcgc
    17101 aagcgccggg gcaacctgcc gcaggtcacg ggaaggcatg ccgcatcgca tagaatcggc
    17161 acgcgccgca ttcacgttcg cgccggcgac acgtcgcccc caacctccgc acaggaacag
    17221 gcatggatcc gatcctcgta ggcaaggccg tcaccaccga aaccagcggc aatgtcttcc
    17281 tgttgccgaa gtacggcaac cgccacggcc tgatcgccgg ggcgaccggc accggcaaga
    17341 cggtcaccct gatgaccctg gccgagggct tcagccggat cggcgtgccg gtcttcctgg
    17401 ccgacgtgaa gggcgacgtg gccgggctgg cggtggcggg cacggcgagc gagaagctgc
    17461 agcagcgcat cgacctgatg ggcatcagcg attaccgcaa cgaggccgcg ccggtggtgt
    17521 tctgggatct ctggggcaag ctgggccatc cggtacgcac caccgtcagc gagatcggcc
    17581 ccaccctgct ctcgcgcatc ctggagttga acgaaaccca ggccggcgtg ctcgacatcg
    17641 tgttcaagct ggccgacgac cgcggcctgc tgttgctcga cctcgacgac ctgcgcgcgc
    17701 tgctcggcct gatcgccgac gagcgcaagg acatcagtac ctcgtatggc ctggtcagca
    17761 cgccgtcgat cggcgcgatc cagcgcgcct tgttgcgcct ggagcaggaa ggcgccgaca
    17821 tgttcttcgg cgagccggcg ctggagctca gtgacctcat gcgcacgacc accgacggcc
    17881 gcggcgtggt caacatcctc gccgccaacc agctgatcct gaagcccaag ctgtactcca
    17941 gtttcctgct gtggctgctg tcggaactgt tcgagaacct cccggaagtc ggcgacctcg
    18001 acaagccgaa gctggtgttc gtgttcgacg aagcgcacct gctgttcgac gacgcgccgg
    18061 ccgcgctgcg ccagcgcatc gaacaggtcg tgcgactgat ccgctccaag ggcgtaggcg
    18121 tgtacttctg ctcgcagttc cccgacgacg tgcccgacga gatcctcggt cagatgggca
    18181 accgcttcca gcacgcgctg cgcgccttca ccccgcgcga ccagaaggca gtgaagacag
    18241 cggccgaaac cttcgtcgcc aatcccgcgc tcgacgtggc cgccacgatc tcgcaactcg
    18301 gcaccggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg aagtacgaca
    18421 accgcatcga ccgcgactcc gccgccgaac tgctggcgaa gaaggccgag gccaaggttg
    18481 cccaggccga ggcgccgccg gccaagaccc gcgaccagga cgacgccgag cagggtggct
    18541 tcggtggcgc cgtcaaggac gcggtgttcg gcaccaagcg ccggcagggc atgatcgaga
    18601 ccatggccaa gcagaccacc cgcacgatcg gcgccaagct gggcaaccag atcgttcgcg
    18661 gcatcctggg cagcatcttc ggcggcaagc gctgatcgac gacgcgcgct ccgctatcac
    18721 cccccatcca tcatccggat atcgtcagca atgaggcttt ccatgaatcg caccctcctg
    18781 gccatcgcgc tcgccgccac cctcgccgcc accgccggct gcaagcccga cgaagccgcc
    18841 gaggccgctg ccgcgccggc cgtcgccgcc gctcccgtca atgcggtgac cgaggtcgtc
    18901 gaccagggtt cgcccgcagg cgacgtcaag ggcttcgaca tcaaggcgtt cgccggcacc
    18961 tacggcggaa ccctgccttg cgcggactgc ccgggcatcg acagcaccat cgcgttcacg
    19021 ccggaaggca cctacacgct gagcgaaacc taccaggaca cggacaagag cagcttcctg
    19081 tccaagggca cctggtcgct gcgcggcgac ggcaaggccg tgctgctgga tccggaggac
    19141 aaggacgaat acgaccgcag cttcagcatc gtctcgccga ccgaactgcg cgcgctggac
    19201 cagcagggca agccgatcga cagcacgctc gactacagcc tcaagcgcaa gtaagcatgg
    19261 ggcgctggcg cccgcccgcg gcgggcagca ccgcgctggt cacccgcgag gggcacgacc
    19321 ggctgaaggc ggaactggac gacctctggc gggtgcgccg gcccgaggtc gtcaaggccc
    19381 tggccgccgc cgccgcggaa ggcgaccgct ccgagaacgc cgagtacacc taccgcaaga
    19441 agcaactcgg cgagatcgac cgtcgggtgc gctacctgag caagcggctg gaagcattgc
    19501 gcgtggtcga cgcggcaccc tccgatcgcg actcggtgtt cttcggcgcg gaggtgacgc
    19561 tggagaacat cgacgatggc cagcaggtcg cctatcgcat cgtcggcccc gacgagaccg
    19621 atgcccggcg gggctggatc agcatcgacg ccccactcgc ccgcgccatg ctcaagaagc
    19681 gcgtggacga cgaattcgat gccgtcctcc cgggcggcat cgtccactac gcgatcatcg
    19741 ggatccgtta cgcggactga tcgcgggtag gcccgccatc gacgtcgggc gtccccacgt
    19801 cgtcatcgtc gtccgccacc ccgcgttcgc gcgcacgccg cgccaacgcc tcgtagcgat
    19861 cctggaacat ccgcaattcg tgctcttcca gctcctcgag gtcgagcagg ctgttgtgcg
    19921 cgcctcgggt ggcgcggatc aattcatcca gcttgatctg cagggctcgc gcgtcgcggt
    19981 tctggctgct ctggatcaga aagaccatca ggaaggtgac gatcgtggtg ccggtgttga
    20041 tcaccagttg ccaggtatcg ctgaagccga acagcggccc ggtgatgatc cagaccagca
    20101 ccaccagcaa cgccagcacg aaactggaag gactgccggc gaagcgcgag gcggcattcg
    20161 ccaggcgggt gaaccaacgc tggtgcgcca tgcggcttgc tcccggaccc aagccgccat
    20221 cttcgccccg cacgcgcgga ggtcaagcgg ggcccgcgcg gcgctggcgg ctcagtcgtc
    20281 gtattcgact tcgccgtaga gatcgtgttc gtcgctgccc atgatccgaa cgcgggcgaa
    20341 ctcgccgggc accaagccgg cctcgcggcc atcctgcacc tgtaccaggc cgtcgatctc
    20401 cggcgcatcc gccatcgagc gcgcgatggc gagttcgccg tcgatcgcat ccaccagcac
    20461 ctgctgcacc gtgcccactt tcgccgccaa ccgcgcctcg gaaatcgccg cctgacgctc
    20521 catgaaaagc gccaggcgct cctgcttgac cgcttcgtca accggatccg gaagctcgtt
    20581 tgccttcgcg ccatccaccg gcgaataggc gaacgcaccg acgcgatcga gttgggcttc
    20641 gtcgaggaag tccagcagct cctcgaactc cgcctcggtt tcgcccggga agccgacgat
    20701 gaaggtgctg cggatggtga gttccgggca ggtcgcgcgc cagcgctgca cgcgctccag
    20761 ggtcttgtcg accgcacccg gccgcttcat cagcttcagc acgcgcgggc tggcgtgctg
    20821 gaacgggatg tcgaggtatg gaaggagctt gccgtccgcc atcaacggga tgatgtcgtc
    20881 cacgtgcgga tacggataca cgtagtgcag gcgcgtccac gcatcgagtt cggacaggcc
    20941 ttcgcacaac gccttcatcc gcgtctggta cgtcttgtcg cgccaggtgc gctcggcgta
    21001 cttgacgtcc acgccgtaag cgctggtgtc ctgcgacacc accagcagtt cgcggacgcc
    21061 gccgcgcacc agcttctcgg cctcgcgcag tacctcgtcg accgggcgcg acaccaggtc
    21121 gccgcgcatc gaagggatga tgcagaagct gcagcggtgg ttgcagcctt ccgaaatctt
    21181 caggtacgcg tagtgcttcg gcgtgagctt gagcccgtaa tcgggcaaca ggttgacgaa
    21241 gggatcgtgc cgcggcggca gcgcggtgtg caccgcgtcc atcacgctgg cgtagtcctg
    21301 cgggccgctg atcgacagca cgcccggatg cgcctcgcgg atcacctcgg cgcgcttgcc
    21361 caggcagccg gtgacgatga ccttgccgtt ctccgccatc gcctcgccga tcgcatccaa
    21421 cgactccgcc accgcgctgt cgatgaagcc gcaggtgttg accaccacca cgtcggcgtc
    21481 gtcataggcc tgcaccaggt cgtagccctc gacgcgcagt tgggtgagga tgcgctcgga
    21541 gtcgaccagc gccttggggc agccgaggct gacgaatccg actttgggtt gttgcaggga
    21601 catgcgcggg gcaccacgaa aggtgcgaaa ttatacggcg ggcatcccct gccccggcgg
    21661 cctcaccacc tcatcgcgct ccgcgatcgg aggacatcat gggcgaatgg atccacctcg
    21721 ataccgccgc aggcccggcc cgggcctggc tcgcgcgtcc ggcgaagccc ccgcgcgggt
    21781 cggtgatcgt gctgcaggag atcttcgggg tgaacgcgca catccgcgcg gtgaccgatg
    21841 gattcgctgg acacggcgtc gtcgccctgg cgcccgccct gttcgacccg gtccagccgg
    21901 acgtcgagct gggctatggc gatgccggcg tcagccgtgg ccgcgaactg gtgtccgagt
    21961 tgggtaccga tggcgccctg caggtggtca ccgccgctgc gcgctggctg cgcgaggccg
    22021 gccaccatcc cggcgtggtc ggctattgct ggggcggctc gatcgccttc ctcgcaaata
    22081 cccggctcgg cctgccagcc gtgagctact acggcgcacg gacgatgccg ttcatcgccg
    22141 agcccgcacg cgccgccctg atgttccact tcggcgggcg cgacgccagc atcccggcgg
    22201 acgacatcgc ccgccaccgc gcactgcagc cacgggccga ggccttcgtc tatcccgagg
    22261 ccggccacgc cttcaaccgc gacatcgacc cgatccattt ccacgcggcg agcgccgcgc
    22321 tggcacgacg gcgcacactc gccttcctgg cggagcacac ctcatgaacc actggcatct
    22381 gcacccgcaa ctggccgacg acaccacccc ggtgatcgaa ctcgcgctct gcgaagtccg
    22441 cctgatggac gacgccaacc acccgtggct ggtgctggta ccgcgggttc ccgacctggt
    22501 cgagatcatc gacctggatg ccacgcagcg cgcccagctc acgacggaaa tcgatgtcgc
    22561 atcgcgcgcg ctcaaggcgc tgttcaagcc gcacaagctc aacatcgccg cgcttggcaa
    22621 cctggtgccg caattgcacg tgcacgtgat cgcgcgctac accgacgaca tcgcctggcc
    22681 gcgccccgtc tggggggcag cgaacgcccg accctatgcg ccggaggaac tgatcgagcg
    22741 ggtgactgcg ctgcgcgccg cgctcgcggg gtaagactca ggtcttcggc aacgtcacgc
    22801 cagtctgccc ctggtacttg ccgccgcggt ccttgtaact ggtctcgcag acgtcgtcgc
    22861 catcggcctg gaagaacagc atctgcgcca cgccttcatt ggcgtagatg cgtgccggca
    22921 gcggcgtggt attgctgaat tccagggtca cgtggccttc ccactccggc tccagcggag
    22981 tgacattcac gatgatcccg cagcgcgcgt aggtgctctt gcccaggcac acgacaagcg
    23041 tgtcgcgcgg gatgcggaag tactcgacgg tgcgcgccag cgcgaacgaa ttcggcggga
    23101 tgatgcattc gtccgccacc acgtcgacga agctcttcgg gtcgaagtgc ttgggatcga
    23161 cgatggtcga gttgatgttg gtgaagatct tgaactcgcg cgagcagcgc acgtcgtagc
    23221 cgtagctgga ggtgccgtag ctgacgatgc ggtggccgtc cacctgcttg acctggccgg
    23281 gctcgaacgg ctccagcatg ccgtgctccc gggccatgcg gcggatccag cggtcgctct
    23341 tgatgctcat cgggtcgggt cttgttgcgg ggacgggccc gcgattctaa ctgggcggcc
    23401 tgcggcttcg ggaagccgga tcagctgccc agcagcgagg ccatgatccc gctgcgcagc
    23461 ttcgggcgct tgtcgagctg ggcaagcatg gcccgggcga tgtcgcgata cgcctgcgac
    23521 ggggccgcat ccggctgcgc cgccaccatc ggcatgccgg catcgccctg ctcgcggatg
    23581 ccgatctcca gcggcagcga gcccagcacc ggcacaccgt attgggcgga catgcgcgcg
    23641 gcaccgccct cgccgaacag atgctcggca tggccgcact gcgtgcagac gtgtactgcc
    23701 atgttctcga ccaggccgag caccgcgatg ccgagcttct cgaacatctt cagcgccttg
    23761 cgcgcgtcca gggtggcgat gtcctgcggc gtggtcacga tcaccgcgcc cgccaccggg
    23821 attttctgcg ccatggtcag ctggatgtcg ccggtgcccg gcggcaggtc gaccaccagc
    23881 acgtcgagat cgccccagcg ggtttgctcc agcagttggg tcagcgacga ggtcgccatc
    23941 ggcccgcgcc agatcatcgg cgtgtcctgg tcgaccagca ggccgatcga catcgcctcg
    24001 atcccatgcg cgcggatcgg ctcgatggtc ttgccgtccg gactgtccgg ccgacccgac
    24061 aggccgagca tcatcgggat gctggggccg tagacgtcgg catccagcac gcccacggcc
    24121 aggccttccg cggcgagcgc cagcgccagg ttcacggcgg tcgtggactt gcccacccca
    24181 ccctttccgg agccgacggc aatgatgttg cggacatggg gcagcggctt gagttcgccc
    24241 tggacggcgt ggggggtgag gcggggcatg ggggacgcct tgctgcgggg cggcaagtct
    24301 agcgccgact gacatccgga tcgtgcaggt cgtggtgcct gatgtgccat tggatcagaa
    24361 gcgcacatgc ctcatggtcg ccgacctgct cgacctggat cctccgttcg cgaccgcggg
    24421 catcggcagc gacggctgcc cgggccatcg atgcaacctg tcgttcgggc gcaaggcttg
    24481 atgggcgagg gccggtgaca agcctccgtg aagaagcggc ccggcgacca acagtcccgg
    24541 ttgtgaaaca ggcaagtctc ggactgcata cggctgcgcg gctctggcac atgcttcgag
    24601 tgatatccgt ggtcaaccgc cggcattccg tgtggcgtac tcgttaattt ggtgttacgt
    24661 tcaggtggcc aaccgattgg taactcgcaa gtccatgtat ttgctcacat ttttgaagga
    24721 gacctgaatg accgctcgcc aacgccgact tatccaacga agccccttga cactcgcgct
    24781 cgccgccgcg ctctttttgc cggttgcgca tgcacaggac caggcctcgc aggatcagtc
    24841 cgccgctacc gccgaccaga agccgaagga cctcgacaag gtcacggtca ccggctcgct
    24901 gatcccgcaa accacgctgg aaaccttcac cccggtcacc atcatctcgg ccgaggacat
    24961 ccaggcgcgc ggcttcaaca acgtcgctga agtgctgcag aagagctcgt tcgcaaccgg
    25021 tggcgtgcag aacaaccaga gctccgcatc gtttacccaa ggcgccgaga ccctgagcct
    25081 gttcggcctt ccgtccggct acgtgaagta cctgatcgac ggccgtccga tggccaacta
    25141 cccggcgctg tacaacggct ccgacgtgtt caacaacatc agtggcatcc cggtcgagtt
    25201 ggtcgagcgc atcgagatcc tgccgggcgg ccagtcctcg ctgtacggct cggacgcgat
    25261 cgccggcgta gtcaacatca tcctgaagaa gaacatcgat ggtggcgtgg tcaccgcgcg
    25321 cctgggcggt tacgacgagg gcggtggtcg cagcggccgc atcagcctcg ccgacagctt
    25381 cagcagcaag gacgggcgct ggaatacgct ggtcggcatc caggccgaga agaacgatcc
    25441 ggtctggggc taccagcgcg acctgacccg ccagttcaac accgatgcct acaacggtgc
    25501 cgccccgctg gcgagccgcg actggctggt ctacagcccg ttcaccagct acaagttcct
    25561 ggatccctcg aactgcgcga acgtgtccgc cggcttcgac gggagcgtcg gattgcagac
    25621 ccgccccggc ttcggcgacg agaagtattg cggttcgatg acgacccccg gctaccgcac
    25681 cctgaagaac agcaaggacg ggatccagct ctatacccac accacgttcg acttcaacga
    25741 cctggcccag ggctacgccg acgtcctgct gagccgcgag aaggtccgtt accacatcgg
    25801 ctcgggatac acctggtggg gcaccagcgt cggctggggc tacttctacg acccccgcgt
    25861 cggcacgggt gttgccgatg cctatggcga actgatctgc ggtgatgatc cggattgtct
    25921 ggcatcgttg cccccgggcg acctgctcaa cctgcagcgc gccttcaccc cggaggacat
    25981 gggcggctgg gaaaacacga tggacgagaa caagagttcg tcctactcgg tcacgctcgg
    26041 cgtgaacggc accctgagcg aggattccaa ctgggattac gacgtcagcc tgacccgcac
    26101 ccattacaag ctcaacgaaa tcagcctggc ccgcctggcc gacccgatca acaactactt
    26161 ccagcagcac g
//
LOCUS       DAOOCX010000022        25986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1571_length_25986_cov_8.139061, whole genome shotgun sequence.
ACCESSION   DAOOCX010000022 DAOOCX010000000
VERSION     DAOOCX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 25986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25986
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1571_length_25986_cov_8.139061"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PK399_03590"
     CDS             <1..690
                     /locus_tag="PK399_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011258121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPW12237.1"
                     /translation="IFAAGLGERMRPLTDTTPKPLLEVGGKRLIEWHLEKLAAIGVDD
                     VVINISWLADQFPATLGDGSHWGLRIVYSHEGDTPLETGGGMWNALPLLGDDPFLLVN
                     GDVWTDVDFAGLPRAPIGLAHLVMVDRPPQATHGDFALDDAGFVRADGAHRLTYSGIG
                     VYRPQLFEGWHQIVQAPAIVNGKPRFPLAPLLRAHMADGRITGQHHRGRWTDVGTPER
                     LARLDAELRGG"
     gene            736..1035
                     /locus_tag="PK399_03595"
     CDS             736..1035
                     /locus_tag="PK399_03595"
                     /inference="COORDINATES: protein motif:HMM:NF022786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HPW12238.1"
                     /translation="MSDPQTQFAELSNAFLALANQKLTSDNHTQVSMALLAAAARFNA
                     HVVAAACGNAERARAEAPKASAFLVGQFEKSLAANLGEYIANYDANLQRSGLGHN"
     gene            complement(1021..1743)
                     /gene="hda"
                     /locus_tag="PK399_03600"
     CDS             complement(1021..1743)
                     /gene="hda"
                     /locus_tag="PK399_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010369247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HPW12239.1"
                     /translation="MDDRLGPQLPLALRALPDQRLERYLRAPEGLLEQLRALASGDMV
                     DALYLQGAAATGKTHLLLGSCTLATSLGKQAAYLPLGNVRGRLRVASEGLDADLVAVD
                     DVDAIAGHREDEVALFDLHNRLRDAGRSLLYAASAGPDALPLVLPDLRSRLSQCTRWS
                     LPVLDDGARAELLRQRAAARGLDFDDAALDWMLRRCSRDLGTLTALFERLDRASLAAQ
                     RRLTVPFLRQVLGAEGADSVVP"
     gene            complement(1766..2932)
                     /locus_tag="PK399_03605"
     CDS             complement(1766..2932)
                     /locus_tag="PK399_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPW12240.1"
                     /translation="MSMHPDNDLAAIATFYRRVQWTALGLGVLWVVWLLAPILSPFVF
                     AALLGWLGDPMVDRLEAKGFKRNNAVILVFSAMTLVLVIALIVLVPLLEQQIVTLVES
                     LPNYRDWLVGTALPWVEQRTGLQIVSWLDPERLIQLVREHWESAGGIAATALGYVSRS
                     GFALLGWIANIVLLPVLAFFFLRDWDLLVERVGLLVPRDHYETVARLTRESDAVLGGF
                     LRGQMLVMLILGVLYAVGLSLVGLNLGILIGVIAGLLTFVPYLGPAAIVVFGGTAALV
                     QFGDWQHLAGVAVVFTIGQVIESYWLTPKLVGDRIGLHPMAVIFAVMAGGSLFGFLGM
                     LLALPVAAVVNVLLRYAQERYRQSRLYAGESPAIVVATDVHPVATARDGEPPAA"
     gene            complement(3049..4050)
                     /locus_tag="PK399_03610"
                     /pseudo
     CDS             complement(3049..4050)
                     /locus_tag="PK399_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
     gene            4146..5243
                     /gene="purM"
                     /locus_tag="PK399_03615"
     CDS             4146..5243
                     /gene="purM"
                     /locus_tag="PK399_03615"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009591854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPW12241.1"
                     /translation="MNAAAKIDVSRAREPAVTAHQTPAANGLTYRDAGVDIDAGNEVV
                     ERIKPLVKRTMRPEVLGGVGLFGGLFDLAGRYREPVLVSGTDGVGTKLILAKQLNRHD
                     SIGQDLVAMCVNDVLVQGAEPLFFLDYFATGKLDVETTVQVVAGIAKGCEIAGCALIG
                     GETAEMPDMYPPGEYDLAGFTVGAVEKSKLIDSSKVRAGDVLIGIASSGPHSNGYSLV
                     RKILQRAGTPLDLDLGGVTLADALMEPTRIYVKPVLELLARHDLHAMAHVTGGALVEK
                     IIRVVPKDFGLEIDTSSWPLPAVFDWLQREGNVAREEMWRTFNCGIGYVLMAAPADVA
                     AIEADLDRLGLAHWQIGQVVPAGEGERLRIG"
     gene            5249..5869
                     /locus_tag="PK399_03620"
     CDS             5249..5869
                     /locus_tag="PK399_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HPW12242.1"
                     /translation="MTTTKRVAFGLTLAVFAASWIAPRWPLEQALHSSLTVLGLAWLW
                     RHDRRWPLRPLHFALVCGFVIAHSIGARWLYSNVPYDAWLQAAIGWSPTATFGWERNH
                     FDRFIHLAFGLCFAPPTWAWLRQRWPGLASSQGFALAVVLVMCTSLAYEWLEWAIALA
                     LSPEAAEAYNGQQGDPWDAHADMLLATLGALLAWPLGRTRSTGATP"
     gene            5866..6510
                     /gene="purN"
                     /locus_tag="PK399_03625"
     CDS             5866..6510
                     /gene="purN"
                     /locus_tag="PK399_03625"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPW12243.1"
                     /translation="MTIRIAVLASGRGSNLEAILQAIATGQLDAEVIGVFSDKPEAPA
                     LQRVPPAARWSRDAKAFPDRASFDAALADAVAASRPDWVVCAGYMRILGEAFVQRFRG
                     RLLNIHPSLLPKYRGLQTHARALQAGDAEHGASVHFVVPELDAGTVIMQAVVPVLAGD
                     APDTLAARVLQVEHPLLVAALQLVAAGRMSEQDGVAWLDGQPLFTPLRLQFAAP"
     gene            6561..7238
                     /locus_tag="PK399_03630"
     CDS             6561..7238
                     /locus_tag="PK399_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017168434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPW12244.1"
                     /translation="MKTSRLATVFATLLLATAPAWAVKPFAAEYEANIKGTLQADAQM
                     TLSSAGADRWNYVLSVTSPVASVRQATVFEDRGGDWRPLSGTDTTQMLMKKSQKNATY
                     DWGAGEARWNGDVKADRAGPVKLQSGDLDAMLLNLAIVRDVAAGKPLNYRMVDNGVIR
                     AQTYQNLGKETVTVGGQPRSATKVSRSTENKQVIVWVVDGLPVPARILQKKDGKDELD
                     LVLKSVR"
     gene            complement(7309..8565)
                     /gene="murA"
                     /locus_tag="PK399_03635"
     CDS             complement(7309..8565)
                     /gene="murA"
                     /locus_tag="PK399_03635"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPW12245.1"
                     /translation="MQKIVVTGGARLEGDVRISGAKNAVLPILCATLLADAPVRITNV
                     PRLHDVFTTAKLLAGLGAGIVHEGDAMTVDPRSVNSHIAPYELVKTMRASVLVLGPLL
                     AKFGDAEVSLPGGCAIGSRPVDLHIKGLQALGAEISVDHGFIKARSNGRLKGARHVFE
                     LVSVGATENVLMAAALAEGTTVLENAAMEPEIVDLADCLVAMGARIEGAGTSRIVIEG
                     VDALQGCEHAVIADRIETGTFLVAAAMTGGRVTATHARPDTLDAVLDKLREAGADIGI
                     DGDRITLDMQGKRPRAVNLTTAPHPAFPTDMQAQFMALDCVADGVGVINETIFENRFM
                     HVNELVRLGADIRVDGHTAVVRGSEALSGAPVMATDLRASASLILAGLVADGDTVIDR
                     IYHLDRGYENIEAKLSALGASIRRID"
     gene            complement(8565..8813)
                     /locus_tag="PK399_03640"
     CDS             complement(8565..8813)
                     /locus_tag="PK399_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HPW12246.1"
                     /translation="MNADSIRQMIETGFEKAGLPGARAEVQGEDGVHFEATVVSEAFA
                     GKLPLARHRMVYATLGEKMGGEIHALQLRTVTPAEAAA"
     gene            8868..9860
                     /locus_tag="PK399_03645"
     CDS             8868..9860
                     /locus_tag="PK399_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPW12247.1"
                     /translation="MPQPAHANDFDFAASGRRVFAVEADGLAAVAGRIDGAFSAACRA
                     ILASRGRVVCCGMGKSGHVARKIAATLASTGTPAFYVHPGEAAHGDLGMVTDADIVLA
                     LSYSGESDEVLLLLPALKRQGNAVIAMTGREQSTLAREADIHLDVSVPAEACPLALAP
                     TSSTTASLAMGDALAVALLEARGFTADDFARSHPAGALGRRLLLHITDVMHAGDDVPK
                     VGPDASVSEALVEMSRKRLGMTAVVDADDRLLGLYTDGDLRRSLDDEHVDLRGTRIDA
                     VMTRAPRTIDADALAVEAAQLMETHKINALLVVDGEHRVVGALNIHDLLRARVV"
     gene            9860..10405
                     /locus_tag="PK399_03650"
     CDS             9860..10405
                     /locus_tag="PK399_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPW12248.1"
                     /translation="MTMPAYPADVLERAKKIRLIGFDVDGTLTDGRLFYGTDGHEAKS
                     FQVLDGLGFTLLRHAGIETALVTARISEVVERRGRELQVKHIHTHERSKLACMQRIAT
                     DMGIGMDEVAFVGDDLPDLATMRNVGLAIAPANAHHWVLPAAHWVTPRRGGEGAGRDA
                     CDLLLHAQGHVEGILAHGEHP"
     gene            10402..10971
                     /gene="lptC"
                     /locus_tag="PK399_03655"
     CDS             10402..10971
                     /gene="lptC"
                     /locus_tag="PK399_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPW12249.1"
                     /translation="MTHSPISWRTGLTIALLLGAIASGWSVWQQSRPVDDGVLKTRPD
                     YVLRDFEITSLDKQGKESFTLRGPVLQRDPADKTMELDTPLFLVPDRQNRYWQVSARH
                     GVVPADGSQLQLRGQVLATSPADAPPATRIETEQLNLFPGENRATSSAAVAITRPGLT
                     MNGHGMQADFDRQQISLLSQVRTRYVPQR"
     gene            10955..11479
                     /gene="lptA"
                     /locus_tag="PK399_03660"
     CDS             10955..11479
                     /gene="lptA"
                     /locus_tag="PK399_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HPW12250.1"
                     /translation="MSPSAKSTATLLSTAVLLLAAGIADARTSDRNKPMDIEAAHQVC
                     GLGANDSCTYTDVTMTQGSLRIRAAKAVIQQVNGDMSRVQMSGGVTLSQQLDDGTDFD
                     SKSSAVDYDLRNETVVFTGNVAITQQRGTLNGERVVYNMKSGQVESGGTGNGRVKMRI
                     LPKSAQGAAAKAEG"
     gene            11479..12198
                     /gene="lptB"
                     /locus_tag="PK399_03665"
     CDS             11479..12198
                     /gene="lptB"
                     /locus_tag="PK399_03665"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPW12251.1"
                     /translation="MLVAEGLRKRYRSREVVRDFGLTLNAGEVVGLLGPNGAGKTTCF
                     YMIVGLVPADAGRIVLDGVDITDRPMYARAQLGVGYLPQEPSVFRKLSVADNIRLVLE
                     LRPDLDDDARETELSALLDELQINHVADQAGASLSGGERRRVEIARALAGKPRMILLD
                     EPFAGVDPISVHEIQRIVRHLKQRGIGVLITDHNVRETLGICDRAYILAEGGVLAQGS
                     PAELLANPDVRRVYLGDAFRL"
     gene            12271..13692
                     /locus_tag="PK399_03670"
     CDS             12271..13692
                     /locus_tag="PK399_03670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPW12252.1"
                     /translation="MKPRLSPALQQHLVLTPQLRQAIRLLQLSTVELEAEVAEAVASN
                     PLLDWAEEGEPTPGEGHDARDADAPPETLTLAAEAEDHWDRDAEPWQERIGPSSDRDG
                     NDEMEHPAVAESLRDHLLWQLHLGHFSQRDTNIGIALIDAIDDDGYLREDLEELARSL
                     RPDIDAGLDEFLQVLHRIQQFDPVGVGARDLGECLRLQLRALPADTPGRDVAILVTAG
                     PLQRLPKIGIDGVCQELRCDRGEAESAVQLLRSLDPRPGSQHGELPAGTYVTPDCVIW
                     RQQGLWRVALAAGAMPRVAIQHGYEQMIRHASNTDADYLRGRLQEARWLLKGLESRAD
                     TLLRVVRCLVREQAAFLEFGEQALHPLTLRSIAAELGLHESTVSRAIARKYVRTPRGT
                     IPLRDFFASGIETEGGGSASSTAIQDMIRKLVAAEDPRKPLSDARLADTLKADGVPVA
                     RRTVAKYREALHIPASHERVRIG"
     gene            13757..14083
                     /gene="raiA"
                     /locus_tag="PK399_03675"
     CDS             13757..14083
                     /gene="raiA"
                     /locus_tag="PK399_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPW12253.1"
                     /translation="MQIETHGQQIEVTPALRDYVENRFTRLGRHFEHPCQIRVQLGLD
                     KPHHRAEATVTMTGRSLHADANGVDMYAAIDLLSDKLDRLLMKQKEKMVDHHRGEGIA
                     HSDVLG"
     gene            14138..15091
                     /gene="hprK"
                     /locus_tag="PK399_03680"
     CDS             14138..15091
                     /gene="hprK"
                     /locus_tag="PK399_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPW12254.1"
                     /translation="MNAARITAAELFGKQQDRLELRWLAGQKDGARRVLEAVETVARR
                     PSLAGYLNTIYPNRVQILGTEELAWLDGLDARQRWETIQKIMDVRPLALVISKAQTCP
                     EDLRIAAEESETPLWVSPRRGHELLNHLQYVLARTLAPRITLHGVFMEIYSIGVLVTG
                     ESGSGKSELALELITRGHRLVADDAPEFTQIAPDVLDGTCPDLLQDMLELRGLGVLNI
                     RQMFGDTAVKRNKYLRLIVHLSKPSLEPSPTGMERLTGDLGTRRVLDLDVPTITLPVM
                     AGRNLAVLTEAATRMHILRSKGVDPAAAFMARHSHFLERAE"
     gene            15088..15972
                     /gene="rapZ"
                     /locus_tag="PK399_03685"
     CDS             15088..15972
                     /gene="rapZ"
                     /locus_tag="PK399_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPW12255.1"
                     /translation="MTAENAPPPATPPLVIVSGMSGSGKSVALKTFEDLGYYCVDNLP
                     AELLPEFVRRLQAGQASPDRIAVGIDARSLGDLSDVPEALSQIGAQGVDTRLLFFDTR
                     DEVLLRRYADTRRRHPLSHLGLVLADAISLERQALKPLRQIADHVLDTSELNVHQMRR
                     HILTEYGLSGTGLSLLFESFAYRRGVPAVADFVFDARVLPNPHWDARLRPLSGRDGAV
                     ADHLDADPDVIAYVAQVQGFLDTWLPKLRSETRSYVTVAFGCTGGRHRSVYLAERLAR
                     HCRESGWAEVAVHHRELD"
     gene            16130..16522
                     /locus_tag="PK399_03690"
     CDS             16130..16522
                     /locus_tag="PK399_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit family protein"
                     /protein_id="HPW12256.1"
                     /translation="MAVGILLITHEGIGSALLAVTTRLLRNLPLACEAFEVPFDGDPD
                     ALLPQASAALRRVDGGHGVLVLTDLYGATPSNLAARLSRLGTPVRRVSALSLPMLLRV
                     MNYADLELSELPAVAAAGSRNGVIHDDA"
     gene            16544..16813
                     /locus_tag="PK399_03695"
     CDS             16544..16813
                     /locus_tag="PK399_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPW12257.1"
                     /translation="MIRRELLISNRLGLHARATAKLVQLLSTFRAQATLEARGREVNA
                     KSIMGVMLLAAGPGSTVVLRVDGEDEQAAADAAAALFERRFDEDS"
     gene            16813..18534
                     /gene="ptsP"
                     /locus_tag="PK399_03700"
     CDS             16813..18534
                     /gene="ptsP"
                     /locus_tag="PK399_03700"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPW12258.1"
                     /translation="MRHLLSGNAAARGSALGRARVRLPHALDVEETRITAADVDAELE
                     RIHEAVDIVRAEMHTLRQRLHGALAHEVGEFVDLHALLLDDPELLQGIDALIRTGLYS
                     ADYALRLQRDRIAAVFEGMDDAYLRSRIDDIDQVMGRLHAALHRREADLQGVAGEILV
                     TDSIAPAELAQLQAQGVVGVVTAGGSPLSHSAILARSLHLPLVVGATQALLKVNDGDV
                     LVIDGASGLVVLEPNAADLRAHHKRVIESKRERRELNRLRREPSRTLDGVDIKLWANA
                     ESRDDVAEAHALGAAGVGLYRTEFLFMGSSELPDEETQFRAYRDAVIGMTGRTVTIRT
                     LDLGADKADKTGLALRDEPNPALGLRGVRLSLAREGLLRTQLRAMARASGYGPLRALV
                     PMVSGYEEMRTVRRALDAVLLELRSEGFEVADHVPLGAMIEVPSAAISLPDFIGLCDF
                     LSIGTNDLVQYLLAADRTNEALGELYSPLHPAVLRVLRDVIRIANRRGKPVAVCGEIA
                     GDPMFTPLLLALGIGELSLHPATMLEVRRAVRGCDLSALRAAAPALFRARERAGIEAW
                     LKAHGAR"
     gene            18751..20112
                     /gene="mgtE"
                     /locus_tag="PK399_03705"
     CDS             18751..20112
                     /gene="mgtE"
                     /locus_tag="PK399_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPW12259.1"
                     /translation="MAEAVRHDKTARQLRLLSDALDSGRLGPVRRLVNTLSPAEIGNL
                     LESLPPGKRTVVWGLVDAEDDGEVLVHVGDEVRESLIADMDPDELVAAVEDLDIDDLA
                     DLVEDLPDTVIDEVLKSMDRENRERLEQVLSYDEDSAGRLMNPDVVTVRADTTIDVVL
                     RYLRLRGELPEHTDHIYVVSKRHQYLGRIALQALLTHEPGTPINQLLDDEQPAIGVDE
                     TSDEVARQFSDHDWISAPVVDDNNILLGRITIDDVVDIIREQAEHQALGAAGLDEDED
                     LFSPVHRAFRNRLIWLGVNLCTAFLAASVVGRFSGSIEQLAALAVLMPIVAGMGGNAG
                     TQVLALMIRGLALGQIGASNVPTLVWKEVRVAMINGLVLGTVLGIVVLAWFRNPALAA
                     VIATALTLNLCFAALGGVFVPVTLKRLGFDPALAGGVILTTITDVMGFLTFLGLATLV
                     LLR"
     gene            20160..20966
                     /locus_tag="PK399_03710"
     CDS             20160..20966
                     /locus_tag="PK399_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HPW12260.1"
                     /translation="MRLPSPRFATRVLAIAVLLLGGCVSAPMRNTGSFVDRDVDIAGI
                     THRYQVFVPASAAGGRRPPVIVFLHGSGERGNDGIKQTEVGIGPYIRAHLTDFPAIVV
                     FPQAPDETEWGGNADLVFATLDAATLEFHGDPDRTYLTGLSMGGYGTWELAMRAPARF
                     AALAPVCGGVVHPRRPSMGVSGLAGVGDPYAAVAARLKDTPTWQFHGALDDVVSPDFS
                     RRMDAALKAAGARDARLTIFPDANHNSWDAAYSQMPELWTWLFAQRRTQR"
     gene            20996..21700
                     /locus_tag="PK399_03715"
     CDS             20996..21700
                     /locus_tag="PK399_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW12261.1"
                     /translation="MLMLAAWPACAQVEIRTTAVERVDFQKQVLGAQHRDTVARAVRA
                     TKRGEREEALEIVRPVLAYCDALLGTGRALVSVSTEAEYREHVATAGNGDPVDWVDMA
                     CPDAYQVHAFVAVEEKRFDDALAMLEKTIRLAPYWSEPLTEHGFVLNQLGRPADGLAS
                     YRSALVLSRKHEANKRAIALALRGIGYSQVELGDLDGAEQSYKESLEVEPGNTLAQRE
                     LDYIRQQREKTPAAKP"
     gene            complement(21697..22644)
                     /locus_tag="PK399_03720"
     CDS             complement(21697..22644)
                     /locus_tag="PK399_03720"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPW12262.1"
                     /translation="MDPQTDVDGRLRHLLSLQGLSRTQLETLLRRAQGLTAHAYGGTG
                     ARHALAGKAVCTLFFEPSTRTRSSFSLAAQRLGADLLNFDASTSSTTKGEADIDTLKT
                     LEAMGVQAFVVRHKRDGAVAELAAAANPGTVLLNAGDGRSNHPTQGLLDMLTLRQAKG
                     DDFSRLKVLVVGDVRHSRVARSDLHALRTLGCGEIRVCGPAALLPDDDTLQGCMVGDD
                     VDAMLDGVDAVMTLRLQRERMVEGLVASLDDYHRDFGLTSTRLRRAADDAIVMHPGPM
                     NRGVEISDEVADGPQSRVLRQVANGVAVRMAVLEALLSD"
     gene            complement(22702..23181)
                     /gene="ruvX"
                     /locus_tag="PK399_03725"
     CDS             complement(22702..23181)
                     /gene="ruvX"
                     /locus_tag="PK399_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPW12263.1"
                     /translation="MSASGDGGPAIRPDGTVLGFDVGARRIGVAIGSAFGSGARPVAV
                     VDVRDGAPDWPRIDQLVREWRPDGVVVGDPMTLDGGDQPIRKAARAFARDLRERTRLP
                     VVMIDERNSSIEAAQRFADARAGGRKRRRDADALDAMAAAIIVERWLAAPQDAVAVA"
     gene            complement(23178..23744)
                     /locus_tag="PK399_03730"
     CDS             complement(23178..23744)
                     /locus_tag="PK399_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002802054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HPW12264.1"
                     /translation="MHEHGHSFGNNLLIALPSLQDSQFERSVTLICQHDGDGAMGVVV
                     NRPSEYTLGEVLQQMGISSDSESLQSQIVLAGGPVHPERGFVLHDGDREWDSTLAVGD
                     GLFLTTSRDVLEALARGEGPGQAVVALGCAGWGAGQLEQELVDDSWLMVPTRRDVLFD
                     LPLPQRWQAAAGSIGVDLVNVAGHSGHA"
     gene            complement(23800..24930)
                     /locus_tag="PK399_03735"
     CDS             complement(23800..24930)
                     /locus_tag="PK399_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPW12265.1"
                     /translation="MSSIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKLSPITQAP
                     LTPQQSRDLVLNVMTPQQREEFEKTHECNFAIGVSGVGRFRVSCFYQRNQVGMVLRRI
                     ETRIPTVEELNLPPIIKTLAMTKRGLIIFVGATGTGKSTSLAAMIGYRNHNSTGHIIT
                     IEDPIEFVHKHDGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAI
                     AFAETGHLVLCTLHANNANQAMDRIINFFPEDRRNQLLMDMSLNLKGVVAQQLIPTPD
                     GKGRRVAMEILLGTPLVQDYIRDGEIHKLKDVMKESVQLGMKTFDQSLFELYQAGEIS
                     YEDALRFADSSNEVRLRIKLAQGGDARTLAQGLDGVEIAELR"
     gene            complement(24983..>25986)
                     /locus_tag="PK399_03740"
     CDS             complement(24983..>25986)
                     /locus_tag="PK399_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPW12266.1"
                     /translation="NKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHSLVYDIMS
                     DKQRRDYEEFLEVDFSFEIPGLARFRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPRI
                     FKELIDQPQGLILVTGPTGSGKSTTLAAMLDHVNKNEYAHILSVEDPIEFVHTSQKCL
                     INQREVHRDTHGFSEALRSALREDPDYILVGEMRDLETIRLALTAAETGHLVFGTLHT
                     SSAAKTIDRIIDVFPAGEKPMVRSMLSESLRAVISQALLKKTGGGRTAAWEIMVGTPA
                     IRNLIREDKVAQMYSAIQTGQNVGMMTLDQHLQDLVKRGMVSRQQAKEYAKDKRLFE"
BASE COUNT         4164 a         9167 c         8658 g         3997 t
ORIGIN      
        1 atcttcgccg ccggcctggg cgagcgcatg cgcccgctga ccgacaccac gccgaaaccg
       61 ttgcttgaag tcggcggcaa gcgcctcatc gaatggcatc tcgaaaagct cgccgcgatc
      121 ggcgtggacg atgtcgtgat caacatctcc tggctggcgg atcagttccc ggccacgctg
      181 ggcgacggat cgcactgggg cctgcgcatc gtctactcgc acgaaggcga caccccgctg
      241 gaaaccggcg gcggcatgtg gaatgccttg ccactgctcg gcgacgatcc gttcctgctg
      301 gtcaatggcg acgtctggac cgacgtcgat ttcgcaggcc tgccacgcgc accgatcggc
      361 ctcgcccatt tggtgatggt ggatcgcccg ccgcaggcca cgcatggcga cttcgccctg
      421 gacgacgccg gcttcgtccg tgccgatggc gcgcatcgcc tgacctattc cggcatcggg
      481 gtgtaccggc cgcagttgtt cgagggttgg caccagatcg tgcaagcccc cgccatcgtc
      541 aacggcaagc ccaggttccc gctggcaccg ctcctgcggg cacacatggc cgacggccgc
      601 atcaccggcc aacaccaccg cgggcgctgg accgatgtcg gcacgccgga acgcctggcc
      661 aggctggacg cggagctgcg cggcggctga gcacgatgcg ctacccttgc caccccacgc
      721 acgcatggca ccacgatgtc cgacccgcag acccagttcg ccgaactcag caacgccttc
      781 cttgccctgg ccaaccagaa gttgacctcg gacaaccaca cccaggtctc gatggcgctg
      841 ctggccgcgg ccgcgcgctt caacgcccac gtcgtggctg cggcctgcgg caacgcggag
      901 cgcgcgcgcg ccgaggcgcc caaggcgagc gccttcctgg tcggtcaatt cgagaagagc
      961 ctggcagcga acctgggcga gtacatcgcc aactacgacg ccaacctgca gcgcagcggc
     1021 ctagggcaca actgagtcgg cgccctccgc gcccagcacc tggcggagga agggcacggt
     1081 caggcgacgc tgcgccgcca acgaggcccg atccaggcgt tcgaacagcg cggtgagggt
     1141 gccgaggtcg cgactgcaac ggcgcagcat ccagtccagc gcggcgtcat cgaaatccag
     1201 gccccgcgcg gcggcacgct ggcgcagcaa ctccgcacgc gcgccgtcat ccagcaccgg
     1261 caggctccac cgcgtgcatt gcgacaggcg cgagcgcagg tcgggcagga ccagcggcaa
     1321 ggcgtcgggt ccggcagacg cggcatacag caggctgcga ccggcatcgc gcaggcggtt
     1381 gtgcaggtcg aacagggcga cttcgtcctc gcgatgaccg gcgatcgcat cgacatcgtc
     1441 cactgccaca aggtccgcat ccaagccctc gctggcgacg cgcaggcgtc cgcgcacgtt
     1501 ccccagcggt agatacgcgg cctgcttgcc caggctggtg gcgagcgtgc aggaaccaag
     1561 cagcaagtgg gtcttgccgg tcgcagccgc cccttgcagg taaagcgcat cgaccatgtc
     1621 gccgctcgcc agtgcccgta gttgttcgag caagccttcg ggagcacgca ggtaacgctc
     1681 cagccgctgg tccggcagtg cgcgcagggc gagcggcaac tgcgggccga gcctatcgtc
     1741 caccagcatg ttgtccaccg ccatctcagg ccgcaggcgg ttcgccatca cgtgcagtcg
     1801 cgaccggatg cacgtcggtg gcaaccacga tcgccggact ctcgcccgcg tacaggcgac
     1861 tctgccgata gcgttcctgc gcgtagcgca gcagcacgtt gaccaccgcc gccaccggca
     1921 gggccagcag catgccgagg aaaccgaaca ggctgccgcc ggccatcacc gcgaagatca
     1981 ccgccatcgg gtgcaggccg atgcggtcgc cgaccagctt gggcgtgagc cagtagctct
     2041 cgatgacctg gccgatggtg aacaccaccg ccacgccggc caggtgctgc cagtcgccga
     2101 attgcaccag cgcggcggtc ccgccgaaca ccacgatcgc cgccgggccc aggtagggca
     2161 cgaaggtgag caggccggcg atgacgccga tcaggatgcc caggttcagg cccaccagcg
     2221 acaggccgac cgcatacagc acgccgagga tcagcatgac cagcatctgc ccgcgcagga
     2281 agccgccgag caccgcatcg gattcgcggg tcagcctggc caccgtctcg tagtggtcgc
     2341 gcggcaccag caacccgacg cgctcgacca gcaggtccca gtcgcgcagg aagaagaacg
     2401 ccagcaccgg cagcagcacg atgttggcga tccagccgag cagggcgaag cccgagcgcg
     2461 agacgtagcc cagcgccgtc gcggcgatgc cgcctgcgct ctcccaatgc tcgcgcacca
     2521 actggatcag ccgttccggg tccagccacg agacgatctg cagcccggtg cgttgctcca
     2581 cccacggcag cgcggtgccg accagccagt cgcggtagtt cggcagcgac tccaccaggg
     2641 tgacgatctg ctgttccagc agcggcacca gcacgatcag cgcgatcacc agcaccaggg
     2701 tcatcgcgct gaacaccagg atcaccgcgt tgttgcgctt gaagcccttt gcctccaagc
     2761 ggtcgaccat cgggtcgccc aaccagccca gcagcgcggc gaacacgaac gggctgagga
     2821 tcggtgccag cagccacacc acccacagca cgccgaggcc cagtgcggtc cactgcacgc
     2881 gccggtagaa ggtcgcgatg gcggccaggt cgttgtcggg atgcatgctc agtccaggcg
     2941 gaaggtggcg acttgcggcg ccacggcgtc gccgtcggcg tcatcgctgc gctcgcctgc
     3001 ggagacggtg gacaggacct cgccgcgatc cgcgtatttc gcgaaattgg cgatgccggt
     3061 ggccagttcg aggtcgagtt ccagcacgtc cgccgatgcc gacaccgggg tgacccgctt
     3121 cacgatcgac acgccttcca ggtaaccggc gaggcgcagg taatcgtcgg cgctgtcgat
     3181 gccgaggacg cgcacccggt aacgtcccgg tggaccgccg ctgccggcct tggcgtagcg
     3241 cttgaatagc gcgtccgccg cgccatccgc gccgccggcc atcgcccggc gcgcatccgg
     3301 gtgcttggtc tgccacttcg acagcacctt gcccttgtcg acgaagatcc agtcggcgac
     3361 ccagccgccc gcgccgcgct gcagcttgcc gagcagttgc atcggcgggc tgtaacgctt
     3421 ggacagcgac gcgatcgcgg cggtgtctcc acgccagatt gccccgaccg cggcttgctc
     3481 cgccgcgctg ccggagggca ggcccaaggc atagccgcgc tgcttggcct ggtcgagcac
     3541 cgcgcgcgcg gcattcacct gtgccagcgc caccagccgc gggcccgtgc cgtcgtcgat
     3601 gcccagccac agcaccggct tcgggcgcgg caacggccaa ctgggcaggc ccagcatctg
     3661 gatcaggccg tccacctcgt cgcgcttgaa gcgcaccacc agggtggtct ggaaactcgg
     3721 cgcgccggtg gcggacacgc cttcgtcctg gcggtagtcg taactggcga catgggcctt
     3781 ggccctggcc agttcgtcgc gcacgccggg ctgctgggcg acgccccggt cgccggtggc
     3841 attggccagc acctgcgcca gtgcgcgtcc gaacgcgccg ttgcgctcgc catcggtctg
     3901 gttgcgcacc gcgacctcgg cctcgtaggc gcccgtggcg gcggcacgat cgccctccac
     3961 gcgctgggcc agtgccggca cgcaggcgag caaccacagg caggccgcca ccaggccaac
     4021 caacccccat cgtccgcttg cttgcggcat cgctcgcatc gccaatcctt gcccggaatc
     4081 catgtcctgc gcccgaaatg gtgccgcagc gcggcgggca cgtccatgac cgcgccgtcg
     4141 ccagcatgaa tgcggctgct aaaatcgacg tttcccgcgc ccgtgagccc gccgtgaccg
     4201 cccaccagac tcccgctgcc aatggcctca cctatcgcga tgccggcgtc gacatcgatg
     4261 cgggcaacga ggtcgtcgag cgcatcaaac cattggtcaa gcgcacgatg cgcccggagg
     4321 tgctcggcgg cgtgggcctg ttcggcggcc tgttcgacct ggccgggcgc taccgggaac
     4381 cggtactcgt gtccggcacc gacggggtgg gcaccaagct gatcctggcc aagcagctga
     4441 accgccacga cagcatcggc caggacctgg tggcgatgtg cgtgaacgac gtgctggtcc
     4501 agggcgcgga accgctgttc ttcctcgact acttcgccac cggcaagctc gacgtcgaga
     4561 ccacggtgca agtggttgcc ggcatcgcca agggctgcga gatcgccggc tgcgcgctga
     4621 tcggcggcga gaccgcggaa atgcccgaca tgtacccgcc cggcgaatac gacctggccg
     4681 ggttcaccgt cggcgcggtg gagaagtcga agctcatcga cagcagcaag gttcgtgcgg
     4741 gtgacgtgct gatcggcatc gcatccagtg gcccgcactc caacggctat tcgctggtgc
     4801 gcaagatcct gcagcgcgcg ggaaccccac tggatctcga cctcggtgga gtgacgctgg
     4861 ccgacgcgct gatggaaccc acccgcatct acgtcaagcc ggtgctggag ctgctggcca
     4921 ggcacgacct ccatgcgatg gcccacgtca ccggcggcgc gctggtggag aagatcatcc
     4981 gggtggtgcc gaaggacttc ggccttgaga tcgacacctc cagctggccg ctccccgcgg
     5041 tgttcgactg gctgcagcgc gaaggcaacg tggcccgcga ggaaatgtgg cgcaccttca
     5101 actgcgggat cggctacgtg ctgatggcag cgcccgccga tgtggccgcg atcgaagccg
     5161 acctcgaccg gctgggcctg gcccactggc aaatcggcca ggtcgtgccc gccggcgaag
     5221 gcgaacgcct gcgcatcggc tgagtgcgat gacgaccacg aaaagggtcg ccttcgggct
     5281 caccctcgcc gtgttcgcgg ccagctggat cgccccgcgc tggccgctcg agcaggccct
     5341 gcacagcagc ctgaccgtgc ttggcctcgc ctggctgtgg cggcacgacc gccgctggcc
     5401 attgcgcccg ctgcacttcg cactggtctg cggcttcgtc attgcccaca gcatcggtgc
     5461 gcgctggctt tactcgaacg tgccgtacga cgcctggctg caggcggcca tcggctggtc
     5521 gcccaccgcg accttcggct gggagcgcaa ccatttcgac cgtttcatcc atctcgcctt
     5581 cggcctctgt ttcgcaccgc cgacctgggc ctggctccgc cagcggtggc ccgggctcgc
     5641 gtcgagccag ggctttgcgc tcgcggtggt gctggtcatg tgcaccagcc tggcctacga
     5701 atggctggaa tgggccattg cgctcgccct ctcgccggag gccgcggagg cctacaacgg
     5761 ccagcagggc gatccatggg atgcgcacgc cgacatgctg ttggccacgc tcggcgcatt
     5821 gctggcctgg ccgctgggac gcacccggtc cacgggggca acgccatgac catccgcatc
     5881 gcggtgctcg cctccggtcg cggcagcaac ctggaggcca tcctgcaggc gatcgcaaca
     5941 ggccaattgg atgccgaggt gatcggcgtc ttctccgaca agcccgaggc acctgccctc
     6001 cagcgcgtgc cgccagctgc tcgctggtcg cgcgacgcga aggcattccc ggatcgcgcc
     6061 tcgttcgatg ccgcgctggc cgatgccgtc gcggcatcgc ggccggactg ggtcgtctgc
     6121 gcgggctaca tgcgcatcct cggcgaggcc ttcgtgcagc ggttccgggg acgcctgctg
     6181 aacatccatc cgtcgctgtt gccgaagtac cggggcctgc aaacccacgc gcgtgcactg
     6241 caggccggcg atgccgagca tggcgccagc gtgcatttcg tggtcccgga actcgatgcg
     6301 ggcaccgtga tcatgcaggc ggtggttccc gtgctggccg gcgatgcccc cgacacgctc
     6361 gccgcacgcg tcctgcaggt cgaacacccc ttgctggtcg cggccttgca actggtggca
     6421 gcgggccgca tgtctgaaca ggatggcgtt gcctggctgg atggtcagcc cctatttacg
     6481 cccctgcggc tacagttcgc cgccccatga atgacgctgg cccggtgcat gccggccccc
     6541 agcgcgacag cgaagacccc atgaagactt cccgcctcgc caccgtgttc gccaccttgc
     6601 tgctcgccac cgccccggcg tgggcggtca agccgttcgc agcggagtac gaagcgaaca
     6661 tcaagggcac cctgcaggcc gatgcgcaga tgaccctgtc ctcggccggt gcggaccgct
     6721 ggaactacgt cctgagtgtg accagcccgg tcgcgtcggt gcgccaggcc accgtgttcg
     6781 aggatcgcgg cggcgactgg cgcccgctct ccggcaccga taccacccag atgctgatga
     6841 agaagtcgca gaagaacgcg acctatgact ggggcgcggg ggaggcacgc tggaacggcg
     6901 acgtgaaggc cgatcgcgcc gggccggtga agctgcagag cggcgacctg gacgcgatgc
     6961 tgctcaacct ggccatcgtc cgcgacgtgg ccgcgggcaa gccgctcaac taccgcatgg
     7021 tcgacaacgg cgtgatccgc gcgcagacct accagaacct gggcaaggag acggtcacgg
     7081 tcggcggtca gccgcgcagc gccaccaagg tcagccgcag caccgagaac aagcaggtga
     7141 tcgtgtgggt ggtggatggc ctgccggtgc cggcgcggat cctgcagaag aaggacggca
     7201 aggacgaact cgacctggtc ctgaaatcgg tgcgctgaat cccatcgccc gccacaaacg
     7261 aaaagccccg cgatgcgggg cttttttgtg cgcgcatgtg ccgggcaatc aatcgatccg
     7321 gcgaatgctc gcacccagtg ccgacaactt cgcttcgatg ttctcgtagc cgcgatccag
     7381 gtggtagatg cgatcgatga cggtgtcgcc atcggcgacc agcccggcca ggatcagcga
     7441 ggccgacgcg cgcaggtcgg tcgccatcac cggcgcgcca ctgagcgcct cgctgccgcg
     7501 caccaccgcg gtatgcccat ccacgcggat gtccgcgccc aggcggacca gttcgttcac
     7561 gtgcatgaag cgattctcga agatcgtctc gttgatcacg ccaacgccat ctgccacgca
     7621 gtccagcgcc atgaactgcg cctgcatgtc ggtggggaat gcggggtgcg gggcagtggt
     7681 gaggttcacc gcgcgcggcc gtttgccctg catgtccagg gtaatgcggt cgccgtcgat
     7741 gccgatgtct gcgccggcct cgcgcagttt gtcgagcacc gcgtccagcg tgtcggggcg
     7801 ggcatgggtc gcggtgacgc ggccaccggt catcgccgca gcgaccagga atgtgccggt
     7861 ctcgatgcgg tcggcgatca ccgcgtgttc gcagccctgc agcgcgtcca cgccttcgat
     7921 cacgatccgc gaggtgcccg caccttcgat cctggcgccc atcgcgacca ggcaatcggc
     7981 caggtcgacg atctccggct ccatcgccgc gttttccagc acggtggtgc cttccgcaag
     8041 cgcggcggcc atcagcacgt tctcggtcgc gcccacgctg accagttcga acacatgccg
     8101 cgcgcctttc aaacgaccat tgctgcgcgc cttgatgaag ccatggtcga cgctgatctc
     8161 cgcacccagc gcctgcaggc ccttgatgtg caggtcgacc gggcgcgatc cgatggcgca
     8221 gccgccgggc aacgagacct cggcatcgcc gaacttcgcc agcaacggcc ccagcaccag
     8281 caccgaggcg cgcatagtct tgaccagctc atagggcgcg atgtggctgt tgacgctgcg
     8341 cgggtcgacg gtcatcgcat cgccctcgtg gacgatgccg gcgcccaggc cagcgagcag
     8401 cttggcggtg gtgaacacgt cgtgcaggcg cggcacgttg gtgatccgca ccggcgcatc
     8461 ggccagcagg gtggcgcaga ggatcggcag tacggcgttc ttggcgccgg aaatgcggac
     8521 gtcgccctcg agcctggcgc caccggtgac cacgatcttc tgcatcaggc ggccgcctcc
     8581 gccggggtga cggtgcgcag ctgcaacgca tggatctcgc cacccatctt ctcgcccagg
     8641 gtggcgtaga ccatgcggtg acgggccagc ggcagcttgc cggcgaacgc ctcgctcacc
     8701 acggtcgcct cgaagtggac gccgtcttcg ccctgcacct cggcgcgggc gccgggcagg
     8761 ccggcctttt caaagcccgt ctcgatcatc tggcggatgc tgtcggcgtt catggcgtat
     8821 gcggtgcgct caagtaaact gaacgaccga gtttagcgga atccctagtg ccccagcccg
     8881 cccacgccaa tgacttcgat ttcgccgcca gcgggcgtcg cgtgttcgcg gtcgaggccg
     8941 atggcctggc ggccgtcgcc gggcgcatcg acggggcgtt ttctgccgcc tgccgggcga
     9001 tcctggccag ccgcggccgg gtcgtctgct gcggcatggg caagtccggc cacgtggccc
     9061 gcaagatcgc ggcgaccctg gcatcgaccg gcacccccgc gttctacgtg cacccgggcg
     9121 aggccgccca cggcgacctg ggcatggtca ccgatgccga catcgtgctg gcgctgtcgt
     9181 actccggcga aagcgacgag gtgctgctgc tgttgccggc gctcaagcgc cagggtaacg
     9241 ccgtcatcgc gatgaccggc cgtgagcagt ccaccctcgc ccgcgaagcc gacatccacc
     9301 tggacgtcag cgtccccgcc gaagcctgcc cgctggcgct ggcgcccacc tccagcacca
     9361 ccgcctcgct ggcgatgggc gatgcgctcg ccgtggcctt gctggaggcg cgcggcttca
     9421 ccgccgacga tttcgcccgc tcgcatccgg ccggcgcact gggccggcgc ctgttgctgc
     9481 acatcaccga cgtcatgcat gccggcgacg atgtcccgaa ggtcggcccg gatgccagcg
     9541 tcagcgaggc gctggtcgag atgagccgca agcgcctggg catgaccgcc gtcgtggatg
     9601 ccgacgaccg cctgctcggc ctctacaccg acggcgacct gcgccgcagc ctggacgacg
     9661 aacacgtcga cctgcgcggc acccgcatcg acgcggtgat gacccgcgcg ccgcgcacca
     9721 tcgacgccga cgccctggcg gtggaggccg cgcaactgat ggaaacccac aagatcaatg
     9781 ccctgctcgt cgtcgacggc gagcaccggg tggtcggcgc actgaacatc cacgacctgc
     9841 tgcgcgcgcg ggtggtgtga tgacgatgcc cgcctacccc gccgacgtcc tcgaacgtgc
     9901 gaagaagatc aggctgatcg gcttcgatgt cgacggcacc ctgaccgacg gtcgcctgtt
     9961 ctacggcacg gacggccacg aggccaagtc cttccaggtg ctggacggcc tcggtttcac
    10021 cctactgcgg catgccggca tcgagacggc cctggtcacc gcgcggatca gcgaagtcgt
    10081 ggaacgccgc ggccgcgaat tgcaggtcaa gcacatccac acccacgagc gcagcaaact
    10141 tgcctgcatg cagcgcatcg cgacggacat gggcatcggc atggacgaag tggcgttcgt
    10201 cggcgacgac ctgccggacc tggccacgat gcgcaacgtc ggcctcgcga tcgccccggc
    10261 gaatgcccac cactgggtgc tgccggccgc gcactgggtc accccgcgcc gcggcggcga
    10321 aggcgccgga cgcgatgcct gcgacctgct gctgcatgcg cagggccatg tcgagggcat
    10381 cctggcccac ggggaacatc cgtgacccac agccccatca gctggcgtac cggcctgacc
    10441 atcgccctgc tgctcggcgc gatcgccagt ggttggtcgg tctggcagca atcgcggccg
    10501 gtcgacgacg gcgtgctcaa gacccgtccc gactacgtgc tgcgcgactt cgagatcacc
    10561 tcgctggaca agcagggcaa ggaatccttc accctgcgcg ggccggtgct gcagcgcgat
    10621 cccgccgaca agaccatgga actggacaca ccgctgttcc tggtcccgga tcggcagaac
    10681 cggtactggc aggtcagcgc caggcacggc gtggtcccgg ccgacggcag ccagttgcag
    10741 ttgcgtggcc aggtgctggc gaccagccca gccgatgcgc cgccggccac ccgcatcgag
    10801 accgaacagc tcaacctgtt ccccggcgag aaccgggcga cctcgtccgc cgcggtcgcg
    10861 atcacccgcc ccggccttac aatgaatggc catggcatgc aggcggactt cgaccgccag
    10921 caaatctccc tcctgtcgca ggtgcgtacc cgttatgtcc cccagcgcta agtccaccgc
    10981 cacgctgctg tccaccgcgg tccttctcct cgccgccggc attgccgatg cgcgcacctc
    11041 cgaccgcaac aagccgatgg acatcgaggc cgcccaccag gtctgcggcc tgggcgccaa
    11101 cgacagctgc acctacaccg acgtgaccat gacccagggc agcctgcgga tccgtgcggc
    11161 caaggcggtg atccagcagg tcaacggcga catgagccgg gtccagatga gcggcggggt
    11221 caccctcagc cagcagctcg acgacggcac cgacttcgat tcgaagtcct ccgccgtgga
    11281 ctacgacctg cgcaatgaaa ccgtggtgtt caccggcaac gtggcgatca cccagcagcg
    11341 cggcaccctc aacggcgaac gcgtggtcta caacatgaaa tcgggccagg tcgaaagcgg
    11401 tggcaccggc aacggccggg tcaagatgcg catcctgccg aagagcgcgc aaggcgcggc
    11461 ggccaaggcg gaaggctgat gctggttgcc gagggcctgc gcaagcgcta ccgctcgcgt
    11521 gaagtggtgc gcgacttcgg gctgaccctc aacgccggcg aagtggtcgg cctgctcggc
    11581 cccaacggcg cgggcaagac cacctgcttc tacatgatcg tgggcctggt cccggccgat
    11641 gccggccgca tcgtgctgga tggcgtcgac atcaccgacc gcccgatgta cgcccgcgcg
    11701 caactcggcg tgggctacct gccgcaggaa ccctcggtgt tccgcaagct cagcgtggcc
    11761 gacaacatcc gcctggtgct ggaactgcgc ccggacctcg acgacgacgc tcgcgaaacc
    11821 gagctgtccg ccctgctcga cgaactgcag atcaaccatg tcgccgacca ggccggcgcc
    11881 agcctgtccg gcggcgagcg ccggcgcgtg gagatcgccc gggccctggc cggcaagccg
    11941 cgcatgatcc tgctcgacga gccgttcgcc ggcgtcgacc cgatctcggt gcacgagatc
    12001 cagcgcatcg tgcggcacct caagcaacgc ggcatcggcg tgctgatcac cgaccacaac
    12061 gtgcgcgaga ccctgggcat ctgcgaccgc gcctacatcc tcgccgaggg cggcgtgctg
    12121 gcccagggct cgcccgccga attgctggcg aacccggatg tgcggcgcgt gtacctgggc
    12181 gacgccttcc gcctgtgact gcggcgatgc accgcccgtg gctgcctcgc taacggtttt
    12241 ttacccgcgt ctggcgatat gctggggacg atgaagccgc gcctctcccc cgcgctgcaa
    12301 cagcacctgg tcctgacgcc gcaactgcgg caggcgatcc gactgctgca gctgtccacc
    12361 gtcgaactcg aagccgaagt ggccgaagcc gtggccagca atccgctgct ggactgggcg
    12421 gaagagggcg agccgacgcc gggcgaaggg catgacgcgc gggacgccga tgcaccgccg
    12481 gaaaccctca ccctggccgc cgaggccgaa gaccactggg atcgcgatgc agaaccgtgg
    12541 caggagcgca tcggcccctc gtcggatcgc gacggcaacg acgagatgga gcatcccgcg
    12601 gtcgccgaga gcctgcggga ccacctgctg tggcagttgc acctgggcca tttcagccag
    12661 cgcgacacca acatcggcat cgccctgatc gatgcgatcg acgacgacgg ctacctgcgc
    12721 gaggacctcg aagaactggc gcgcagcctg cgtcccgaca tcgacgccgg cctggacgaa
    12781 ttcctgcagg tcctgcaccg gatccagcaa ttcgatccgg tgggggtcgg tgcgcgcgac
    12841 ctcggcgaat gcctgcgcct gcaactgcgc gcgctgccgg ccgacacccc cggccgcgac
    12901 gtcgccatcc tggtgaccgc cggcccgctg cagcgcctgc ccaagatcgg catcgacggc
    12961 gtctgccagg aactgcgctg cgatcgcggc gaggcggaat cggcggtgca gttgctgcgc
    13021 agcctggatc cgcgccccgg atcgcagcac ggcgaactcc ccgccggcac gtacgtcacc
    13081 ccggattgcg tgatctggcg ccagcagggc ctgtggcggg tcgcactggc cgccggggcg
    13141 atgccacggg tggcgatcca gcatggctac gagcagatga tccgccacgc cagcaacacc
    13201 gatgccgatt acctgcgcgg tcgcctgcag gaagctcgct ggctcctgaa gggactggaa
    13261 tcgcgcgccg acacgctgtt gcgggtggtc cgctgcctgg tccgcgagca ggccgcattc
    13321 ctcgagttcg gcgaacaggc cctgcacccg ctgaccctgc gcagcatcgc cgcggaactt
    13381 ggcctgcacg aatccacggt gtcccgggcg atcgcgcgca agtacgtgcg cacgccgcgc
    13441 ggcaccatcc cgctgcgcga tttcttcgct tcgggcatcg agaccgaggg cggcggcagt
    13501 gcgtccagca ccgccatcca ggacatgatc cgcaagctgg tggccgcgga agacccgcgc
    13561 aagcccttgt cggatgcccg cctcgccgat acgttgaagg cggacggcgt gccggtggcc
    13621 cggcgcaccg tggcgaaata ccgcgaggcc ctgcatatcc cggcgtcgca cgaacgcgtg
    13681 cgcatcggct gacgccgctg cgtcgcaccc accggcggca tcggtgccgt cggcccacac
    13741 gagcaaggag gtccagatgc agatcgaaac ccacggccag cagatcgaag tcaccccggc
    13801 gctgcgcgac tacgtggaga accggttcac ccggctcggt cggcacttcg agcacccgtg
    13861 ccagatccgc gtgcaactgg gactggacaa gccgcaccat cgcgccgagg cgaccgtcac
    13921 catgaccggc cgcagcctcc acgccgacgc caacggcgtg gacatgtacg ccgccatcga
    13981 cctgctgtcg gacaagctgg atcgcctgct gatgaagcag aaggaaaaga tggtggacca
    14041 ccaccgcggc gaaggcatcg cccacagcga cgtgctgggt tgagccaacc gcgatgaagc
    14101 aaggggaaca ggcccgccgg cgggcggtta tgcgatggtg aacgccgcgc gcatcaccgc
    14161 cgccgagctc ttcgggaaac agcaggaccg gctggaactg cgctggctgg cgggccagaa
    14221 ggacggcgcg cgacgggtgc tggaggcggt agagaccgtc gcccggcgtc cgtcgctggc
    14281 cggctacctc aacacgatct acccgaaccg ggtacagatc ctcggcaccg aggaactggc
    14341 ctggctcgac ggcctggacg cacgccagcg ctgggagacg atccagaaga tcatggatgt
    14401 ccggccgctg gcgctggtga tcagcaaggc ccagacctgc cccgaggacc tgcggatcgc
    14461 cgcggaggaa tcggagacac cgttgtgggt gtccccgcgc cgcggccatg aattgctcaa
    14521 ccacctccag tacgtgctcg cgcgcacgct ggcgccgcgc atcaccctgc atggcgtatt
    14581 catggagatc tactcgatcg gcgtgctggt caccggcgag tccggctccg gcaagagcga
    14641 actggcgctt gaactgatca cccgcggcca ccgcctggtc gcggacgacg cgcccgaatt
    14701 cacccagatc gcgccggacg tgctcgacgg cacctgcccc gacctgctgc aggacatgct
    14761 ggaactgcgc gggctgggcg tgctcaacat ccgccagatg ttcggcgaca ccgcggtcaa
    14821 gcggaacaag tacctgcgcc tgatcgtgca cctcagcaaa ccctcgctgg agcccagccc
    14881 gaccgggatg gaacgcctca ccggcgacct cggtacgcgc cgcgtcctcg acctcgacgt
    14941 ccccaccatc accctgccgg tgatggccgg gcgcaacctg gccgtgctca ccgaggccgc
    15001 cacccgcatg cacatcctgc gcagcaaggg cgtggatccg gcggcggcct tcatggcccg
    15061 ccacagccac ttcctggaac gcgcggaatg acggccgaaa acgccccgcc gcctgccacg
    15121 cccccactgg tcatcgtcag cggcatgtcc ggctccggca agtcggtcgc gctgaagacc
    15181 ttcgaggacc tgggctacta ctgcgtcgac aacctgccgg cggaactgct gccggaattc
    15241 gtgcgtcgcc tgcaggccgg ccaggcatcg cccgaccgga tcgcggtggg catcgatgcg
    15301 cgcagcctgg gcgatctctc cgacgtgccc gaagccctct cgcagatcgg cgcgcagggc
    15361 gtggacacgc gcctgctgtt cttcgacacc cgcgatgaag tgctgctgcg acgctacgcg
    15421 gacacccgcc gccggcatcc gctcagccac ctcggcctgg tgctggccga tgcgatctcg
    15481 ctggagcggc aggcgctgaa gccgctgcgg cagatcgccg accacgtgct cgacaccagc
    15541 gagctcaacg tccaccagat gcgccggcac atcctcaccg aatacggcct cagtggcacc
    15601 ggcctgtccc tgctgttcga gtcgttcgcc tatcgtcgcg gcgtgccggc cgtcgccgat
    15661 ttcgtgttcg atgcgcgggt gctgcccaac ccgcattggg atgcgcggct gcggccgttg
    15721 tcggggcgcg atggcgcagt cgccgaccac ctcgacgccg atcccgatgt catcgcctat
    15781 gtcgcccagg tgcagggatt cctggacacc tggctgccga aactgcgctc ggaaacccgc
    15841 agctacgtga ccgtcgcctt cggctgcacc ggcggccgcc atcgctcggt ctatctcgcg
    15901 gagcggctgg cccggcactg ccgcgaatcc ggttgggcgg aagtggccgt gcaccaccgc
    15961 gaactggatt gagttggttt tcccttctcc cgcaggcggg cgaagttgcc gaaggcggat
    16021 gcgggtggcg ccatatacga aaccaccctc gccccacccc tctgctgcca acgggagagg
    16081 gagacaatca cgcttccgcg ccgtcatccc cgtctgttaa cttgcggtca tggccgtcgg
    16141 catcctcctc atcacccacg aaggcatcgg cagcgccctg ctggcggtga ccacgcgcct
    16201 gctgcgcaac ctgccgctgg cctgcgaagc gttcgaggtc ccgttcgacg gcgatccgga
    16261 cgcattgctg ccgcaggcca gcgccgcgct gcgacgggtc gatggcgggc acggcgtgct
    16321 ggtattgacc gacctctatg gcgccacgcc cagcaacctg gcggcgcgct tgtcgcggct
    16381 cggcacaccg gtgcggcgcg tgtccgcact cagcctgccg atgctgctgc gggtgatgaa
    16441 ctacgccgac ctggaacttt cggaactgcc ggccgtcgcc gccgccggtt cccgcaacgg
    16501 agtgatccat gacgacgcct gagccacgcc acggaggcgc ccgatgatcc gccgcgaact
    16561 cctcatcagc aatcggcttg gcctgcatgc gcgcgccacc gccaagctgg tgcaactgct
    16621 gtccacgttc cgcgcgcagg ccaccctgga agcacgcggc cgcgaggtca acgccaagag
    16681 catcatgggg gtgatgctgc tcgccgccgg acccggcagc acggtggtat tgcgggtcga
    16741 cggcgaggac gaacaggccg ccgccgatgc cgccgccgcg ctgttcgagc gccgcttcga
    16801 cgaggacagc tgatgcgcca cctcctgtcc ggcaatgccg cggcccgcgg cagcgcactc
    16861 ggacgggcac gcgtgcgcct gccgcacgcc ctggatgtcg aagaaaccag gatcactgcc
    16921 gccgacgtcg acgcggaact cgagcgcatc cacgaagcgg tcgacatcgt ccgcgccgaa
    16981 atgcataccc tgcgccagcg cctgcatggc gcgctggcgc atgaggtcgg cgagttcgtc
    17041 gacctgcacg cgctgctgct ggacgacccc gaactgctgc agggcatcga cgcgctgatc
    17101 cgtaccgggc tctactccgc cgactacgcg ttgcgcctgc aacgggatcg catcgcggcc
    17161 gtgttcgaag gcatggacga tgcctacctg cgcagccgca tcgacgacat cgaccaggtg
    17221 atgggccgcc tgcatgccgc cctgcaccgc cgcgaggccg acctgcaggg cgtggccggc
    17281 gagatcctgg tcaccgacag catcgcgccc gcggagctcg cgcagttgca ggcgcagggc
    17341 gtggtcggcg tggtcaccgc cggcggtagc ccgctctcgc acagcgcgat cctggcgcgc
    17401 agcctgcacc tgccgttggt ggtcggcgcc acccaggccc tgctcaaggt caacgacggc
    17461 gacgtgctcg tcatcgatgg cgccagcgga ctggtggtgc tcgagccgaa cgccgccgac
    17521 ctgcgcgcgc accacaagcg ggtcatcgaa tccaagcgcg agcgccgcga actcaaccgc
    17581 ctgcgccgcg agcccagccg cacgctggac ggcgtcgaca tcaagctgtg ggcgaatgcc
    17641 gaatcgcgcg acgacgtcgc cgaggcccat gcgctcggcg ccgccggcgt cggcctgtac
    17701 cgcaccgaat tcctgttcat gggcagcagc gaattgccgg acgaggagac ccagttccgt
    17761 gcgtatcgcg atgccgtcat cggcatgacc ggacgcaccg tcaccatccg cacgctcgac
    17821 ctgggcgcgg acaaggcgga caagaccgga ctggccctgc gcgacgaacc caatcccgcc
    17881 ctgggcctgc gcggcgtgcg cctgtcgctg gcgcgcgaag gcttgcttcg cacgcagttg
    17941 cgggcgatgg cgcgcgcctc cggctacggc cccttgcgcg cgctggtgcc gatggtcagc
    18001 gggtatgaag agatgcgcac ggtccgccgc gcgctggatg cggtgttgct cgaattgcgc
    18061 agcgaaggct tcgaggtcgc cgaccacgtg ccgctgggcg cgatgatcga agtaccgtcg
    18121 gcggcgatct cgctgccgga cttcatcggc ctgtgcgatt tcctgtcgat cggcaccaac
    18181 gacctcgtgc agtacctgct ggccgccgat cgcaccaatg aggcgctggg cgaactgtat
    18241 tcgccgctgc acccggcggt gctgcgggtg ctccgcgacg tgatccgcat cgccaaccgg
    18301 cgcggcaagc cggtggcggt gtgcggcgag atcgccggcg acccgatgtt caccccattg
    18361 ctgctcgcgc tcggcatcgg cgaactcagc ctgcatccgg cgacgatgct ggaagtacgt
    18421 cgcgcggtgc gcggctgcga cctgtcggcc ctgcgcgcgg ccgcgccagc cctgttccgc
    18481 gcacgcgagc gcgccggcat cgaggcctgg ctgaaggcgc acggcgcacg ctgatcgcgg
    18541 catcggttaa cggggccagc gtgaagtttt tcgaccgggg acatccggtc atgagggtct
    18601 gcatcggcga aacatccacc ctggcccttt caccggtgct ggccggccgt catccccgcg
    18661 ggcgataatg cgcggaccat gaacacctga tcctgtcgcg cacttcgcgt cgcgaccccg
    18721 cccatcgctc cttgaagccg ggaagccgca atggccgaag ccgtccgcca cgacaagacc
    18781 gcgcgccagc tgcgtctgct ttcggacgcg ctggacagcg gtcggctggg gccggtgcgg
    18841 cggctggtca ataccttgtc gcccgcggag atcggcaacc tgctggagtc gctgccgccg
    18901 ggcaagcgta ccgtggtctg gggcctggtc gatgccgagg acgatggcga ggtgctggtc
    18961 cacgtcggcg acgaggtgcg tgaaagcctg atcgcggaca tggatccgga cgagctggtc
    19021 gcggcggtcg aggacctcga catcgacgac ctcgcggacc tggtcgaaga cctgccggac
    19081 acggtgatcg acgaagtcct gaagtcgatg gaccgcgaga accgcgagcg cctggaacag
    19141 gtgctgtcct acgacgagga cagcgccggc cgcctgatga atccggacgt ggtgacggtg
    19201 cgcgccgaca ccaccatcga cgtggtcctg cgctacctgc gcctgcgcgg cgaactgccc
    19261 gagcacaccg accatatcta cgtggtcagc aagcgccacc agtacctcgg ccggatcgcg
    19321 ctgcaggcgc tgctcacgca tgagcccggc acgccgatca accagttgct ggacgacgaa
    19381 cagccggcga tcggcgtgga tgaaacctcg gacgaagtcg cacgccagtt ctccgaccac
    19441 gactggatct cggcgccggt ggtcgacgac aacaacatcc tgctcggccg catcaccatc
    19501 gatgacgtgg tcgacatcat ccgcgaacag gccgagcacc aggcgctggg cgccgccggc
    19561 ctggacgagg acgaggacct gttctcgccg gtgcaccgcg cgttccgcaa ccgcctgatc
    19621 tggctgggcg tgaacctgtg caccgcgttc ctggcggcgt cggtggtggg acgtttctcc
    19681 ggttctatcg aacaactcgc cgcgctggcg gtgttgatgc cgatcgtcgc cgggatgggc
    19741 ggcaacgccg gcacccaggt gctggccctg atgatccgcg gcctcgcgct ggggcagatc
    19801 ggcgcgtcca acgtgcccac cctggtgtgg aaggaagtcc gggtggcgat gatcaacggg
    19861 ctggtgctcg gcaccgtgct cggtatcgtg gtgctggcgt ggttccgcaa cccggcgctg
    19921 gccgcggtga tcgccaccgc gttgacgctc aacctgtgct ttgccgcgct cggcggcgtg
    19981 ttcgtgccgg tcacgctcaa gcgattgggc ttcgacccgg cgctggccgg cggcgtgatc
    20041 ctgaccacga tcaccgacgt gatgggcttc ctgaccttcc ttggcctggc cacgttggtg
    20101 ctgttgcgct gacctggtgc cggtgcgcta atgaccgcca cccggcccgg agtgacgcaa
    20161 tgcgcctgcc atcgcccaga ttcgcgacac gtgtactcgc catcgccgtc ctgctgcttg
    20221 gcggctgcgt gagcgcgccg atgcgcaaca ccggcagttt cgtcgaccgc gacgtcgaca
    20281 tcgccggcat cacccatcgc tatcaggtgt tcgtgccggc atccgccgcc ggcggacgca
    20341 ggccaccggt catcgtgttc ctgcatggct cgggcgagcg cggcaacgat ggcatcaagc
    20401 agacagaggt cggcatcggg ccgtacatcc gcgcgcacct gaccgacttc cccgccatcg
    20461 tggtgtttcc gcaggcaccg gacgagaccg agtggggcgg caacgcggac ctggtcttcg
    20521 ccaccctcga tgccgccacc cttgagttcc atggcgatcc ggatcgcacc tacctcaccg
    20581 gcctgtcgat gggcggctat ggcacgtggg aactggccat gcgcgcaccg gcgcgcttcg
    20641 cggcactggc cccggtatgc ggcggcgtgg tgcatccgcg gcggccgtcg atgggcgtga
    20701 gcgggcttgc gggcgtgggc gatccgtatg ccgcggttgc cgcgcgcctc aaggacacgc
    20761 cgacctggca attccacggc gcgctggacg atgtcgtgtc gcccgacttt tcacgacgca
    20821 tggatgccgc cctgaaggcc gccggcgcac gcgatgcgcg cctgaccatc ttccccgacg
    20881 ccaaccacaa cagttgggat gcggcttact cgcaaatgcc ggaactctgg acttggttgt
    20941 ttgcgcagcg gcgcacgcag cgatgatggg ttggctgcag cgtattgcct tgaccatgct
    21001 gatgttggcc gcttggcccg catgtgcaca ggtcgagatc cgcaccaccg ccgtggagcg
    21061 cgtcgacttc cagaagcagg tgctcggcgc gcagcaccgc gacacggtcg cgcgagccgt
    21121 gcgcgcaacc aagcgtggcg agcgggaaga ggcgctcgag atagtgcgtc ccgtgctggc
    21181 ctactgcgat gcgttgctgg gcacgggacg tgcattggtc agcgtgtcca ccgaagccga
    21241 gtaccgcgag cacgtggcga ccgcggggaa cggagacccg gtcgactggg tcgacatggc
    21301 ttgccccgac gcctatcagg tgcatgcctt cgtcgcggtc gaggagaagc gcttcgacga
    21361 tgcgttggca atgctcgaaa agaccatccg gttggcgccc tactggtcgg agccactcac
    21421 ggagcacggt ttcgtgctga accagttggg gaggcccgcg gacgggctgg ccagctatcg
    21481 gtcggcgctc gtgttgtctc gcaagcatga ggcaaacaaa cgcgccatcg cgctggcgtt
    21541 gcgcgggatc ggctacagcc aggtcgaatt gggcgacctc gacggcgccg agcagtcgta
    21601 caaggaatcg ctggaggtcg agcccggcaa tacactcgcg cagagggaac tcgactacat
    21661 ccgccagcaa cgcgagaaga cgccggccgc gaagccctag tcggacagca gcgcctccag
    21721 caccgccatc cgcactgcca cgccgttggc gacctgccgc aacacccgcg actgcgggcc
    21781 gtcggcgacc tcgtcgctga tctccacgcc gcggttcatc ggacccgggt gcatgacgat
    21841 ggcgtcgtcg gccgcccggc gcaggcgcgt ggaggtcagg ccgaagtcgc ggtggtagtc
    21901 gtccagcgag gcgaccaggc cctcgaccat gcgttcgcgc tgcaggcgca gggtcatcac
    21961 cgcatccacg ccatcgagca tggcatcgac atcgtcgccc accatgcaac cctgcagggt
    22021 gtcgtcgtcc ggcagcaacg cggccgggcc gcacacgcgg atctcgccgc agcccagcgt
    22081 gcgcaacgca tgcaggtcgc tgcgcgccac ccgcgagtgg cggacatcgc cgaccaccag
    22141 caccttgagc cgcgagaagt cgtcgccctt cgcctgccgc agggtcagca tgtcgagcag
    22201 gccctgggtg ggatggttgc tgcggccgtc gcctgcattc agcagcaccg tgccgggatt
    22261 ggccgcggcc gcgagctcgg ccaccgcccc gtcgcgcttg tggcgcacga cgaatgcctg
    22321 cacgcccatc gcctcgagcg tcttcaaggt gtcgatgtcg gcctcaccct tggtcgtgga
    22381 cgaggtggag gcgtcgaagt tgagcaggtc ggcacccagc cgctgcgccg ccagcgagaa
    22441 actcgaacgg gtgcgggtgg acggctcgaa gaacagggtg cagaccgcct tgccggccag
    22501 cgcatggcgc gccccggtgc caccgtacgc atgcgccgtc agcccctgcg cgcggcgcag
    22561 caaggtctcc agttgcgttc gcgacaggcc ctgcaggctc aacaggtggc gcaggcggcc
    22621 atcgacatcg gtttgcggat ccatggggcc tcgcgtggtc gtgttcatcg ggacatgctc
    22681 accagggatg tgctcatcgg gtcatgccac cgccaccgca tcctgcggcg cggccagcca
    22741 gcgctcgacg atgatcgccg ccgccatcgc atccagggca tcggcatcgc ggcggcgctt
    22801 gcgccccccg gcgcgcgcat cggcgaagcg ttgcgcagcc tcgatcgaac tgttgcgctc
    22861 gtcgatcatc accaccggca ggcgggtgcg ctcccgcagg tcgcgggcga aggcgcgggc
    22921 ggccttgcgg atcggctggt cgccgccgtc cagggtcatg gggtcgccga ccaccacgcc
    22981 atccggtcgc cactcgcgga ccagctggtc gatgcgtggc cagtccgggg cgccgtcgcg
    23041 cacatcgacc acggcgacgg gacgcgcgcc gctgccgaag gcgctgccga tcgccacgcc
    23101 gatccgccgt gcgcccacgt cgaacccgag cacggtgccg tcgggccgga tcgcgggccc
    23161 gccgtcgccg gacgcgctca cgcgtgaccg ctgtggccag ccacgttgac caggtccacg
    23221 ccaatgctgc cagccgcggc ctgccaacgt tgaggcagtg gcagatcgaa caggacgtcg
    23281 cggcgggtcg gcaccatcag ccagctgtcg tcgaccagct cctgctccag ttgaccggca
    23341 ccccagcccg cgcaacccag tgcgaccacg gcctgccccg ggccttcgcc ccgcgccaat
    23401 gcctcgagta cgtcgcgcga ggtggtcagg aacagcccat cgccaaccgc cagggtcgaa
    23461 tcccattcgc gatcgccgtc gtgcaggacg aagccacgct ccggatgcac cggcccgccg
    23521 gccaggacga tctgcgattg cagggattcg ctgtcgctgc tgatgcccat ctgctgcagc
    23581 acctcgccga gggtgtattc ggaagggcgg ttgaccacca cgcccatcgc gccgtcgccg
    23641 tcgtgctggc agatcagggt cacgctgcgc tcgaactggg agtcctgcag ggacggcagc
    23701 gcgatcagca agttgttgcc gaagctatgg ccgtgttcat gcatgccggc attgtagcgt
    23761 ggcgacccgc cacgccacag caaggcggca cccctgccct caacgaagtt cggcgatctc
    23821 cacgccatcc aggccctgcg ccagggtgcg ggcatcgcca ccctgcgcca gcttgatgcg
    23881 caggcgcact tcgttggacg agtcggcgaa gcgcaatgcg tcctcgtagc tgatctcgcc
    23941 ggcctggtag agctcgaaca ggctctggtc gaaggtcttc atgcccagtt ggaccgactc
    24001 cttcatcacg tccttgagct tgtggatctc gccgtcgcgg atgtagtcct ggaccagcgg
    24061 cgtgcccagc aggatctcca tcgcaacgcg ccggcccttg ccgtcggggg tcgggatcag
    24121 ctgctgcgcg accacgccct tgaggttcag cgacatgtcc atcagcaact ggttgcggcg
    24181 gtcttccggg aagaagttga tgatgcggtc catcgcctgg ttggcgttgt tcgcatgcag
    24241 ggtgcacagc accaggtggc cggtttccgc gaaggcgatg gcatggtcca tgccttcgcg
    24301 ggtgcgcacc tcgccgatca tgatcacgtc gggtgcctgg cgcagggtgt tcttcagcgc
    24361 gttttcccaa ctgtcggtat cgatgccgac ctcgcgctgg gtgatgatgc atccgtcgtg
    24421 cttgtgcacg aactcgatcg gatcctcgat cgtgatgatg tgcccggtcg agttgtggtt
    24481 gcggtagccg atcatcgcgg ccagcgacgt cgacttgccg gtgccggtgg cgcccacgaa
    24541 gatgatcagg ccgcgcttgg tcatcgccag cgtcttgatg atcggcggca ggttgagttc
    24601 ttccaccgtg gggatgcgcg tctcgatccg gcgcagcacc atgcccacct ggttgcgctg
    24661 gtagaagcaa ctgacgcgga agcggcccac gccggacacg ccgatcgcga agttgcactc
    24721 gtgggtcttc tcgaattcct cgcgctgctg cggcgtcatc acgttcagca ccaggtcgcg
    24781 cgactgctgc ggggtcagcg gcgcctgggt gatcgggctg agcttgccgt ggaccttcat
    24841 cgacggcggc atgccggcgg tgatgaacag gtccgacgcc ttctggtgcg ccatcagctt
    24901 gaggaacgag gtgaaatcga tgctgctcat gccagtggct ccggttcggg gtcccgcctg
    24961 tcctgtcgca cggcgccgtt cgtcattcga acaggcgctt gtccttcgcg tattccttcg
    25021 cctgctggcg actcaccatg ccgcgcttca ccaggtcctg caggtgctgg tccagggtca
    25081 tcatgcccac gttctggccg gtctggatcg ccgaatacat ctgcgccacc ttgtcctcgc
    25141 ggatcaggtt gcggatggcc ggcgtgccca ccatgatttc ccaggctgcg gtgcggccgc
    25201 caccggtctt cttcagcagc gcctgcgaga tcaccgcgcg cagcgattcc gagagcatcg
    25261 agcggaccat cggcttctcg ccggcgggga acacgtcgat gatgcggtcg atggtcttgg
    25321 ccgcgctgga ggtatgcagg gtgccgaaca ccagatggcc ggtttccgcc gcggtcaatg
    25381 ccaggcggat ggtctccagg tcgcgcattt cgccgacgag gatgtagtcg gggtcttcgc
    25441 gcaacgccga gcgcagcgcc tcgctgaagc cgtgggtgtc gcggtggacc tcgcgctggt
    25501 tgatcaggca cttctgcgag gtatgcacga actcgatcgg atcctcgacc gagaggatgt
    25561 gcgcgtattc gttcttgttg acgtggtcga gcatcgccgc gagcgtggtc gacttgccgg
    25621 aaccggtggg accggtgacc aggatcaggc cctgcggctg gtcgatcagt tccttgaaga
    25681 tgcgcgggca acccaggtct tccagggtca gcacttccga cggaatggtg cggaacactg
    25741 cgccggcgcc acggttctgg ttgaacgcgt tgacgcggaa gcgcgccagc ccggggatct
    25801 cgaacgagaa gtcgacctcg aggaattcct cgtaatcgcg gcgctgcttg tccgacatga
    25861 tgtcgtagac cagcgaatgc acctgcttgt ggtcgagcgc ggggatgttg atgcggcgca
    25921 cgtcgccgtc gacgcggatc atgggcggca ggccggcgga caggtgcagg tccgacgcct
    25981 tgttct
//
LOCUS       DAOOCX010000023        25656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1599_length_25656_cov_9.489512, whole genome shotgun sequence.
ACCESSION   DAOOCX010000023 DAOOCX010000000
VERSION     DAOOCX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 25656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25656
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1599_length_25656_cov_9.489512"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PK399_03745"
     CDS             <1..968
                     /locus_tag="PK399_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005912482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW12267.1"
                     /translation="VTVFDRYEQIGGLMQFGIPSFKLDKAVIATRREVLEGMGVVFKL
                     GVEIGRHLTLEHLLTGYDAVFLGLGAYRYTDGGLPGQDLAGVLPALPFLVQNGRIVTG
                     SDPHGRPIAGWEDTLSMPDLAGKRVVVLGGGDTGMDCVRSAIRLGAASVTCAYRRDEA
                     NMPGSAREVANAREEGVQFLFNRQPLAIDSNDGKAASGVRMVETRLGAPDANGRRNPE
                     PIAGSESVVAADIVIIAFGFSPKVPDWLAAQGVQAQPNGRLAVGGNGRLAYQTGHDKL
                     FAGGDCVRGADLVVTAVQEGRDAAASIARMVLRGVRAIPAALESA"
     gene            1171..1857
                     /locus_tag="PK399_03750"
     CDS             1171..1857
                     /locus_tag="PK399_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HPW12268.1"
                     /translation="MRSAVVLGLCLLPLGGGALAQAKPASSTVTVYRCTDAGGKQSLR
                     DTPCPRGQQQQAREMLRPQDAPKPKPVPVPTPVPGPVAPAPEVRTVYLAPPRPMYECT
                     TPDGDRYTSEDGVGNPRWVPLWTLGYPVVGRPIRPPRSGIGPPSSPPTAGMHPPHGGG
                     HWPVIGGGGTWIRDQCALLPPREACARLRDRRDAIRTRFFNAQEKERDALRLEERGIN
                     ARLDDDCGGR"
     gene            1859..2509
                     /locus_tag="PK399_03755"
     CDS             1859..2509
                     /locus_tag="PK399_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HPW12269.1"
                     /translation="MRRFVIAAVLLLASLGAQAQETVFYKCTDAKGNVSMQNGVPCAA
                     GMKQEVKRIGEVRTVPVPAKKPAVAEAPEAKPEYGEFVMVRGPEVKRAPAPEAAALPA
                     PPALFQCRTWENKDYLGETGTPEPTCAALQVTGIDGSAALGMGSACEMKYDTCTAIPA
                     EQLCAAWYRHLDDAEFKLKYAGDDSRPLRQAAFDAIDKKIKASNCSTIAPAAPGTK"
     gene            complement(2669..3115)
                     /locus_tag="PK399_03760"
     CDS             complement(2669..3115)
                     /locus_tag="PK399_03760"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HPW12270.1"
                     /translation="MADLFALTYLSRAARDLLPEELDSILLQARMFNAAEEITGVLLY
                     GDERFFQLLEGEEASVRKVFDRISRARAHQEIRVLSQGPIAGRFFESWHMGFTHSPAS
                     AMQDLSQLAWEDAMPYTRGEAETSVGLGLVLHYWETWSTDANGSAA"
     gene            complement(3187..3573)
                     /locus_tag="PK399_03765"
     CDS             complement(3187..3573)
                     /locus_tag="PK399_03765"
                     /inference="COORDINATES: protein motif:HMM:NF018697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW12271.1"
                     /translation="MKIQERIRRARRRAGLSQARLAELVNVRRSAVSNWESTSNEVVP
                     SMQSLLAVAKACGVSLEWLGTGRGPISVDADALEDVPTANAELVDVEEERVLLAGFRN
                     LPRRGQQLMIDMVEVMQSGRRRAGKP"
     gene            4170..4358
                     /locus_tag="PK399_03770"
     CDS             4170..4358
                     /locus_tag="PK399_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12272.1"
                     /translation="MSIKKHRHLHPQKDDTGDDGNSRSPVRNTSALTLIRNAGYIRPE
                     PQAGDKGADHCAHIRRAR"
     gene            4842..7421
                     /locus_tag="PK399_03775"
     CDS             4842..7421
                     /locus_tag="PK399_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW12273.1"
                     /translation="MLAGNGSAQRHTRALTLATAEAQRPARRHAGALDATPWLRHAQV
                     AVALVILVFMVAILWGLVRQSRGRSRAEHRLRAIADAVPGTVYVLRRTPRDVFTFEFL
                     GVNAVEILGLEREEVLRDTNAARRVILHEDRDRLATALARSADSMSVLEVDFRVRKPD
                     ASLHWVHSSGIPARRANGDVVWNGYLSDITPIKESEQALHDALRRLDDAHTVASLGDW
                     TCDLATGAVTWSPQIYQMLGRDPALGPPDLAESVALFENGAETISEAFFRAQETGQRQ
                     SYELTARLANGEALTLQVFLLPQVDASGTIVGMRGTIQDISSRKALEDRLYQAKEAAD
                     SANLAKSDFLATMSHEIRTPLNGMLGILELIDQTPISPELRGALDTIRESGHSLQRII
                     DDILDFSKVEAGKLEIRPEPTSLVDLAGALQRAYAGSASSHGLELRHKVDPRISPAVM
                     VDALRLRQILGNFVSNAIKFTPRGHVELRINLTERDEGRESLCFEVQDTGIGISQERQ
                     ERLFQPFEQADIDLASRVSGTGLGLAISRRLARLMGGEVSMASEPGKGTTLFLTLSVL
                     PADPANVAPAPALVPAYAPPSSMVDGALERSAAHAASGAPLVLVVDDHPINRMVMSKQ
                     LHALGYAPEDVESGAEALERWTSGRFALVLTDCNMPRMSGYELARHIRHRESAGGLDR
                     TPIIACSANVIGGAREACLEAGMDDYLPKPTKLMALADIMHRWLPLPAKAEAAPAPPE
                     DLAATDAPVADAPLDMRVLHGISNGDQAIERRALAHFRRINDTDSRLLLEAVERVHMA
                     SIIHLAHRIKGACKFVGAHALASASESLEHAGRNGTTRDLDRLVAQFRTELEKLNAYL
                     DAR"
     gene            7399..>8612
                     /locus_tag="PK399_03780"
     CDS             7399..>8612
                     /locus_tag="PK399_03780"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HPW12274.1"
                     /translation="MPISTHADPLDAPRSHGVPASELCFLVAEDHEFQRSMLVQMLEN
                     LGATGVYEAEDGRAAMEITRELEHPFDIIVTDIDMPGMDGLSFIRRLGEAGVGASLIV
                     TSSLDRPLLDTIETMSAAYGMRLLGAVEKPLSQEQLAELIALHWRAKPNPERPRPGAA
                     AFTLDEILAGLRADEFEPFYQPKVELRTGRVKGAEALARWRHPRHGIILPYAFLGLLE
                     DNAQISELTWSILAQGARDCRSWRDAGLDLDVSVNLSGRQLADPGISDAITWQVINQG
                     LEPKHMILEITESAAITDVGRVLENLTRLRMKGFGLSIDDYGTGYSSMQQLTRIPFTE
                     LKIDQSFVMHAARQESSRLILESSLDMARKLGIVSVAEGVETPDDWNLLRQSDCDLAQ
                     GFHIAKPMPSGDL"
     assembly_gap    8613..8712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8766..12194
                     /locus_tag="PK399_03785"
     CDS             8766..12194
                     /locus_tag="PK399_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPW12275.1"
                     /translation="MRIAGLLLCCCAALLAAGCSGQDAPAPTKPAAIAPSVAPQPAIE
                     PKIVVQLGHQSPVLAVRWIDAGRHLASLARDGSIVLWNVADGTILDHARVPLDPALFE
                     GDEAPLRFHAFADAPDAGTLAITYAGTTDRDTERACPEARQPGTRWCAYALDLATRIV
                     RADPGLPLPAASANTTASYWPISPDGRLRPEPNHANGRRGLFDLSDEHIDFPDPTCVS
                     PQRCRYGVTLLPTGGGAPRALTGEPRSYFLDADLSPDGLRLVRVEGLQSETRARVETL
                     DLASANGDRTFEPARAYHHARWLDATHYLLGSHGYDATNDTDAAMAGFPPALVVDAAC
                     AARGDCPTIDSRWQMRPLADGGFVALGSLASGCFGSGGRRVDDVFCPAAEGHADDGNF
                     TSDPPPTGLAWRAPGARQWQPMAQAALAALAGQVITAIEPSPDHRQLAVATRTWDRAD
                     AADPKQVLRLWLLDLADGVATSPRRLVEIVDPAGGRIFTDAVTIRALSFSADARRIVF
                     THAGSAWADARGLKADADLYLVDTDAGARIRKLRGFARRAVAIGDDRVLGLDDGTLLD
                     LESGRVVARIALPAPLVRAGWIERSRLLWATTDDGAIHFFDAGSGTPQLTLQTFPGNR
                     FFALAPGGRYDSNLGADTALVRWVVPDAPWQSLAAQTFMRDYYEPGLYRRLLDCRAND
                     SCATAFKPLPAIASLNRVLPAVRITGVRAGRDAAEAVVSLEVREGIDAHAPNGKTRSG
                     LYNPRLFRNGRLVATMPVQVDAADGDLTAWRRRNAMQATNGVKRLAFTVPVPTQAGEA
                     AQVFTAYAFNEDRIKGETARFAWTRPAVATRPRRAYVLAIGIDDYDTPRFRLKYAVAD
                     ARLMATRLSAIPGHDTHVLVLAGERDASGRRTRVDRATLGRVLSLLAGTGDREATLAA
                     LRAGGIEAGMLQAATPDDAVIVSYSGHGWANAQGDFYLIPGDGRWADGAATPDLASVF
                     ATADLVTPFQAMQAGEIALVIDACHSAASVADGRFKPGPMGDSGLGQLAYDKGIRILA
                     ATQADDVALEDARLGQGLLTYALAVDGLGDGAADLDRDGRIRIDEWLAYAVRRMPALA
                     SDARVGRIAPGGDGARGITFSDLPANAPARRVQQPALFDFNPQPSIVVLRGKTQ"
     gene            12191..14206
                     /locus_tag="PK399_03790"
     CDS             12191..14206
                     /locus_tag="PK399_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPW12276.1"
                     /translation="MKRATPLAWLILAGLLAGCSPSPAPALPNGDAASTRPAADTRRL
                     HALIVGIDRYRFSDARQPGAVFSDLRGAVGDALRFKQALADVFGVDVDAPAAGACESS
                     NAATTTLTDDCATRARILQALEERIATLRPGDTLLFYFAGHGSRYRDDEARDQDTGYN
                     GTILPADARNPDGSPGDLFDVELKALKDRATARGLYFVSVFDACNSATATRDGAGGQS
                     RSAPPFAGSAPPALTTSASGGDGYWVHLAAAQDGEEAQETGGVGTRAGVFTTALVDAL
                     RMPGMRDATFGDLIQEVRLRVAARGHATQTPSAEGALNAAFGARARTTVLFDVAQSDG
                     TPVLQAGATSGMTRGSRFALYATQADAMAGRARIATASIRRVDGATSTLMLDSEVSGK
                     LPKRLVAEQVAHFLPVDAVTVANDLPTGPARDAAAKAVQATGFASVAATGSTRLVAMD
                     GDPDAVALRADDGLLLAELGRVDDPAFADRLAEALHKIARVQQLLALRTNASASASDP
                     FPIATCIAADGYRPTACPPLQAGGVRRIGPGERITATVINRGTVPAYVYVLAIDPRNA
                     VDVVLPKPGEFDRPLPPNQPYRRSGMGFDTPGAYRFVVLASATPVRADALAQAGTGSR
                     DLAACTSPLERLLCASSEGRRDAGVAAVGDWSATISSVLALPKEPAP"
     gene            14203..15336
                     /locus_tag="PK399_03795"
     CDS             14203..15336
                     /locus_tag="PK399_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPW12277.1"
                     /translation="MNRLRPHASLLVCIASCIGFAACERAQAPSPPASATAKPLAKTT
                     TNDCEARRARHDQQQAGRIVGGEPAKPGSAPWQVELLSSPQYDAADRAYDATLADDDA
                     CKIFLAQRNPYELAHKCGGAWIGDGWVLTAAHCVDDIPGFDGREGNVLTDRRVRLGTQ
                     NLTVDDGLFAIDAVVIHGAYRKNTKHDDIALARVVVDPRIAAFEAAGRLAAVPLVGSG
                     DRDFDPGEALRVTGWGWMGRRDVRDAVSRRDSAKQLQRNPADLQQVGLNYLPDALCVK
                     EYGAIYGPGSLCAGTLATDGRVETGKDSCQGDSGGPLTREEDGGRRSLVGLVSNGKGC
                     GAGIPALYTRVSHYDGWIVAAKAAARSGRVERVAQPADATGSR"
     gene            complement(15367..15669)
                     /locus_tag="PK399_03800"
     CDS             complement(15367..15669)
                     /locus_tag="PK399_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12278.1"
                     /translation="MRNTIAAVLVTGLAFGLVACKKPAEPAAEPAAAATATEPQTGTV
                     ERTDPEPQTGTVERTGPEEQTGTVERTGPEEQTGTVERTGPEEQTGTVERTTPAEQ"
     assembly_gap    15823..15922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15923..17475)
                     /locus_tag="PK399_03805"
     CDS             complement(<15923..17475)
                     /locus_tag="PK399_03805"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HPW12279.1"
                     /translation="MSTRTPPAVDGEPGGPTPTHTVFLSYSRADRKGALAIVRVLEAA
                     GYGVWWDGLLEGGERFSRTTEAALAGARAVVVLWSKTSAGSHWVHDEATRGRESGRLV
                     PLSLDGSLPPLGFGQFQSIDVSHALGKPGSEPMRKLLRAVAALHEGPAPALPVPRAEL
                     ASPTRRRIVVAAGLAVVAAGSGVLLWRARDAGTAARGTDAAGGERRIAVLPFENLSGD
                     PEQRYFSDGLTSEIRAQLSRNVQLQIAGQTSSTKAGEGQGDPRDVARALGVDFLLGGN
                     VQKAGGRVKVAAELTDGATGLSRWVNTWERPLSDIFAVQAEIGAAVATALSAAIDGKA
                     ATAGAQVGGTANVEAFDAFLHGRALYEAGIDEDSDRRALDWFDRAIALDARYAGAHAA
                     RARALVVIGNLYAGHDERVRAYDDAAAAARTATTLAPQFAEGFSALGFALASGKLDMR
                     GARVPFDRSHALAPGDADVLSRYATFRSNLRDHDLATGVILRAVALDPLNARTFRSQG
                     DIRHNAGDHA"
     gene            complement(17472..19427)
                     /locus_tag="PK399_03810"
     CDS             complement(17472..19427)
                     /locus_tag="PK399_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12280.1"
                     /translation="MSRTPAGQSVRFAIGITGHRASNPALAANATQVAATLAGIFAEI
                     ESAIAAERVATPLLEPQPTRLHTLLSAGADQLAASAAHALGWELVAPLPFGQALNVAI
                     NAQPRTHADAVALLAGGDAADADVQARAQDIRRWSARARLFQLADRDAEIAALFEATL
                     ASPDDATQARHFHAAVAAQAALSGRIIVEQSDLLVGVWNNGSRDSVGGTGHTIVRALE
                     LGTPVLLIDPARPRHWSILSSTESLAGWQDQQGQDRERLQAVVGNALRARGVARSLGD
                     EAWHPRGTRASTLYRRIEALFGGEAHPLRSLVQAYEAPERIARGSGAPLVEAASRLPG
                     TDPAFVAAVAHDILPAFARADGISARLSDSYRSGMVANFLLSALAVIVGVAWLPLGID
                     DGKWLFALAEFGLLAAILAITWLGGRRRLHGRWFETRRAAEYLRHAPILLLVGVARPP
                     SHWPRATGTEWPEYAARHAMRAPGLPAMAVTRPFLRAALHGLLAPHVADQRAYHHAKA
                     RRLETVDRHLDRLAETLFKIAVASVATWLLMKAGAALHLLPGDLPHATSKAFAFLGIA
                     LPTLGASIAGIRYFGDFERFAAISEVTAGKLAQVEVRIAQLLAGPDSAIDYEGVSRLA
                     HGIDGIVVDEIESWQAVFSGKPIALPA"
     gene            complement(19725..20096)
                     /locus_tag="PK399_03815"
     CDS             complement(19725..20096)
                     /locus_tag="PK399_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPW12281.1"
                     /translation="MATKKAAPAKKAAAKPAAPKPIKEAMTKSGLVAHIAEVSEVAAK
                     DVRAVMAALEGAIHGSVSKKGAGEFVLPGLLKITSVKVAAKPKRKGINPFTKEEVWFA
                     AKPASTKLKVRPLKKLKDAAL"
     gene            20679..23801
                     /locus_tag="PK399_03820"
     CDS             20679..23801
                     /locus_tag="PK399_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW12282.1"
                     /translation="MASKNFAKGLKRSALTVALGMCFAGGVQAQSTSGAIYGKADAGQ
                     TVTIVSETGLTRTVTADANGRYSAPSLPAGRYKVTAGGDARDVTVLVSSGAQVDFGDA
                     TTTLGTVTVNASSVPVIDVSQTDSRTVFTAEQLQEIAVARDINAVALLAPGVINSTAY
                     NSRSSNVGSFGGAAASENAYYINGFPVTNPLTNIGSTTLGFDSIAQQQVLLGGYGAEF
                     GRSTGGVISIITKRGTNDWKGGVYAIYTPASLRSDFKDIYYPDTGFWNATTHYNTTAG
                     NNPANWTDGQLYDLNRYNKSGTQVYGFYIGGPIIKDRLFIYANAERSKTDSESSRYSA
                     NLLGKTAAGLPALSATSRAQAWGVNESTDPRWTVKLDWNINDDHIIEFTGVQDNNKFE
                     SEYYGYNYDTNERDNVLFNTNKTESKTRLYIGKYTGYLTDNLSVSAMFGQQKIDTLAN
                     PLDGYDPSKTYVSISPTVVPTAFAGISNPQKFGPTYNYDGLDETKARRFDINYSIGDH
                     ELRAGYDYFEAFSYRGDNVVGPTGYYWSYGLASNPNAALDASHYVGSPASGGGLGANG
                     YYVTKNYSEHGGGTTVKQKALFIEDRWHITDNILLSLGLRNDGFTNYNGNGEAYVEQK
                     NNWAPRIGFSWDVNGDSSFKVFGNVGRYHLALPNNVSVRGSNPSLSSAQYFTYTGIAA
                     DGTPTGLTAIPYTNANSPYTCANGGMSSNVECGYPKDPRTIAQIDLKPHYQDEFLVGM
                     ERTETDSFSWGAKATYRDLKNAIDDICPTECFIFNAGTNVATFYEDDGTGNLVAVTHD
                     FAVDFGTAFPKLKRKYLALDMYAQYRKGDFFGKIDYTLSHNWGNAEGQLNSSVDTGAG
                     GQGDVSVTQDWDLPELMFGVNGSLPNNRTHQIKMIGTYRFSEEWRVGGSAIVQSGRPR
                     SCYSYWPYAKPGLYNNAYYSYCGVPGAQTAVNNPNVTPNADYVFSPRGSVGETPWTAT
                     FNANVSYTPNWLDGLTLSMDVLNLLNKQTATGYYERSASSRTTVNPRYGQELYFSAPR
                     SFRFSVRYDF"
     gene            complement(23904..24683)
                     /locus_tag="PK399_03825"
     CDS             complement(23904..24683)
                     /locus_tag="PK399_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW12283.1"
                     /translation="MASHRWTLDGQRALVTGASAGIGLAICRELLGLGAQVLMVARDA
                     DMLEQARAELEDEFPGPEDAVLALPADVTDEEQRAEILDWASDQGGLHLLVNNAGGNV
                     TRPVLDYGEDQWRGIFETNLFSAFELCRGAYPLLAKHATSAIVNVGSVSGITHVRSGA
                     PYGMSKAALHQMTRNLAVEWAEDGVRVNSVAPWYIRTRRTSSKLADPDYLDEVLLRTP
                     LGRVGEPEEVAAAVAFLCMPASSYITGECIAVDGGFLRYGF"
     gene            24747..24962
                     /locus_tag="PK399_03830"
     CDS             24747..24962
                     /locus_tag="PK399_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12284.1"
                     /translation="MRIVSPLACLLVIALAGCSPPKPPEEERRPEPQAAAVVQAADAY
                     KDSARAAVAKTEDAAAKEKAALDAATQ"
     gene            complement(24967..>25656)
                     /locus_tag="PK399_03835"
     CDS             complement(24967..>25656)
                     /locus_tag="PK399_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW12285.1"
                     /translation="GSKIDQLLDTHGAYSIAVRELARREQVGMIDLDALSTAWLRALG
                     DEASKPFYMHVPEQDLTDDTHLQQRGATAVACLVVGGWKALDPAISKTVVRDTDCGAR
                     DTALADRAAQAHPSQVVREADLARQQPGPHGGAGTTTAYPFFADAPGLSFFFRKRVLH
                     KGAGIGLHQHDKDEVYYVVSGKGRYIIDGSIRDVGPGDAMLTRTGSTHSLMQDGDDDL
                     VILLAYPKTAD"
BASE COUNT         4190 a         8851 c         8558 g         3857 t
ORIGIN      
        1 cggtcaccgt gttcgaccgc tacgagcaga tcggcgggtt gatgcagttc ggcattccca
       61 gcttcaagct ggacaaggcg gtgatcgcca cccgtcgcga ggtgctggaa ggcatgggcg
      121 tggtgttcaa gctcggtgtc gagatcggcc gccacctcac gctggagcat ctgctgacgg
      181 gctacgacgc ggtgttcctc gggctgggcg cgtaccgcta caccgatggc ggcctgcccg
      241 ggcaggacct ggccggcgtg ctgccggcgc tgcccttcct ggtgcagaac ggccgtatcg
      301 tcaccggcag cgacccacac ggccgaccga tcgccggctg ggaagacacg ctgtcgatgc
      361 cggacctggc cggcaagcgc gtggtcgtgc tcggcggcgg cgataccggc atggactgcg
      421 tgcgctccgc gatccgcctg ggcgcggcca gcgtgacctg cgcttatcgg cgcgatgaag
      481 ccaacatgcc cggctccgcg cgcgaggtcg ccaacgcccg cgaggaaggc gtgcagttcc
      541 tcttcaaccg ccagccgctg gcgatcgaca gcaacgacgg caaggcggcc agcggcgtgc
      601 gcatggtcga aacccggctg ggcgcacccg atgcgaacgg acgccgcaac ccggagccga
      661 tcgccggcag cgaatcggtg gtcgcggccg acatcgtgat catcgccttc ggcttctcgc
      721 cgaaggtgcc ggactggctg gcggcgcagg gcgtgcaggc gcaacccaac ggacggttgg
      781 cggtcggcgg gaatggcagg ctggcctacc agaccggcca cgacaaactg ttcgccggtg
      841 gcgactgcgt gcgcggcgcc gacctggtgg tgaccgcggt gcaggaaggc cgcgacgccg
      901 ccgcgagcat cgcccgcatg gtgctgcgcg gggtcagggc gatcccggcg gcgctggaat
      961 ccgcctgatc gcgagtaaga gggtcacgga aaaaggggtc agagtagagt tttgcgggcg
     1021 agatacttgg gcaccggatc tccatctccg aaaaactcta ctctgacccc tttttctact
     1081 ttcgcctttt cgcttcccgc cttttcgcaa tcgtgaacgc aaccgccaca cggggttcct
     1141 tgcggcggcc cacgcgccca agataggccc atgcgaagcg ccgtcgtcct cgggttgtgc
     1201 ctgctgccgc tgggcggtgg cgccctcgcg caggcgaagc cggccagcag cacggtcacc
     1261 gtgtaccgct gcaccgatgc cggcggcaag cagagcctgc gcgacacgcc ctgcccgagg
     1321 ggccagcagc aacaggcgcg cgagatgctg cgcccgcagg acgcgccgaa gccgaagcct
     1381 gtccccgtgc cgacgcccgt gcccggcccg gtcgcgcctg cgcccgaggt acgcacggtg
     1441 tacctggcac caccccggcc gatgtacgaa tgcaccacgc ccgacggcga ccgctatacc
     1501 agcgaagacg gcgtcggcaa tccgcgctgg gtaccgctgt ggacgctggg ctatccggtg
     1561 gtgggtcgac ccatccggcc gccgcgcagc ggcatcgggc caccgtcatc cccaccgacg
     1621 gcagggatgc acccaccgca tggcggcggc cactggccgg tgatcggcgg cggcggcacc
     1681 tggatccgcg accaatgcgc cttgctgccg ccacgcgagg cctgcgcgcg gctgcgcgac
     1741 cgccgcgacg cgatccgcac gcgcttcttc aatgcacagg aaaaggaacg cgatgccttg
     1801 cgcctcgagg aacgcggcat caacgcccgc ctggacgacg actgcggtgg ccgctgagat
     1861 gcgccggttc gtgatcgccg ccgtgttgct gcttgcctcg cttggcgcgc aggcgcagga
     1921 aaccgtcttc tacaaatgca ccgatgccaa gggcaatgtg tcgatgcaga acggcgtgcc
     1981 ctgcgccgcc ggcatgaagc aggaagtgaa gcgcatcggc gaagtccgca cggtaccggt
     2041 gccggcgaag aagcccgccg tggcggaagc gccggaagca aaaccggaat acggcgaatt
     2101 cgtgatggtg cgaggccccg aagtgaagcg cgcgcccgcg cccgaagccg ccgccttgcc
     2161 ggcgccaccg gcgctgttcc agtgccgcac ctgggaaaac aaggactacc ttggcgagac
     2221 cggcactccg gaacccacct gcgccgcgct acaggtcacc ggcatcgacg gcagcgccgc
     2281 gctgggcatg ggcagcgcct gcgagatgaa gtacgacacc tgcaccgcga tccccgccga
     2341 gcaattgtgc gcggcctggt atcgccacct ggacgatgcc gaattcaagc tgaagtacgc
     2401 cggcgacgac agccgcccgc tgcgacaagc cgcgttcgat gcgatcgaca agaagatcaa
     2461 ggccagcaac tgctcgacga tcgcacccgc cgcacctgga acgaaataag cgtgccggga
     2521 cgccgctcat gcttgcgcat gccccccgtc caggtggaaa cgagcgactc catcctcgtt
     2581 tgaagcgagg caaccttgca gcgcttggac gcggaacctg cggacaaatc ccgcgaggcg
     2641 gcggaacggg gtccgctttg gcaacgggtc aggccgcgga gccgttggcg tccgtggacc
     2701 acgtttccca atagtgcagc accagtccaa ggccgacgga cgtctccgcc tcgcccctgg
     2761 tgtaaggcat ggcgtcttcc catgccaact gcgatagatc ctgcatcgcc gacgctggcg
     2821 aatgcgtgaa ccccatgtgc cacgactcga agaacctgcc tgcaatcggc ccctggctca
     2881 ggacgcgaat ctcttggtgg gcccgtgcac gggaaattcg atcgaacacc ttcctgaccg
     2941 atgcttcctc gccctccagg agttggaaga agcgctcatc tccgtacagc aggacgcccg
     3001 ttatctcctc ggcggcattg aacatgcgcg cctgaagcag gatcgagtcc agctcctcgg
     3061 gcagcaaatc acgcgccgcc ctgctcaggt aggtgagtgc gaagaggtca gccatgatcc
     3121 tggtgcctgc gccctgacat gcaattcaac cactgcagtc gacgggacgc catcgttcgc
     3181 atgcgatcaa ggcttccccg cgcgccgccg acccgactgc atcacttcca ccatgtcgat
     3241 catgagttgc tggccgcgac gcggcaggtt gcggaagccc gccaacagca cccgctcctc
     3301 ttcgacgtcg acgagttccg cattcgcggt gggcacgtcc tcgagtgcgt cggcatccac
     3361 cgatatcgga ccgcggcccg tgccgagcca ttccagggaa acgccgcagg ccttggccac
     3421 tgccagcagg ctctgcatcg aaggcaccac ctcgttgctg gtggactccc agttcgacac
     3481 ggcgctgcga cgcacgttga ccagttcggc caaccgcgcc tgcgacagtc ccgcgcgccg
     3541 gcgcgcgcga cggattcgtt cctggatctt cactggcacc tccaaggaca ttgaacgtgt
     3601 ctgccatgcg ccatgcgttc aagttgttca caaccactgt ctttcatgcg tgaaacgtcc
     3661 gcccgctgat tggttcgagt cctggcggat caccggatcg acgtgagtgc cgcaggccat
     3721 cccacccgct gcaatggaac acgggaggac atctgcagcg gtggacatcc actggccgcg
     3781 aggccttcga tgcacaggaa gattccggca tcgatccgct gcacgctggc caactcggcg
     3841 agccctgcgg gtggaagcct aacgcgtcga cgcgcgcggc gaatgcgctc ctgcatcgtc
     3901 atgcgcaccc gatgacattc accgtgtctg ccacgaagag tgtcgtcagg ttgtacagaa
     3961 tccatgtcat tccaccgtga aagccaatcc acgcgttgcg ttcatgttgg ggacgacagc
     4021 gggaatggcc tgccgaccga atgtaatttc gcgcggttcg acgcaggatg cctgcagcat
     4081 tcatcgcggc acgtcgctca ctgctcgagg gatcgaatct tcccgggcat cgatgagcgt
     4141 ccatccactc gagacgcgga gcaaccacga tgtccatcaa gaaacaccgg catctccatc
     4201 ctcaaaaaga tgacacgggt gacgacggaa acagccgctc gcccgttcgc aacaccagtg
     4261 cgctgacgct gatccgcaac gccgggtaca tccgacccga gccgcaagcc ggcgacaagg
     4321 gcgcggatca ttgcgcgcac attcgcaggg ccaggtgaac gatggcgacg atcattcgcc
     4381 ggcaccggcc atggttcgga ggccctacct gaagccggca ttccagtgcg acatcgtctt
     4441 cagcaggcga ctcatgccgc cggatacgaa gccggtcatc gcataggcct cgtgtcgttc
     4501 gcagattggc ggaaccggcc cgacccgggc acgaacgctt ccggccctcg cgatccccga
     4561 caggggatgg agggccattg acgatgtaaa cagggcatcg gcaatcccgc caacccgtgg
     4621 aggaaggcgg ccgcaccagg tcgggatcca gcacgggatg cggcacgtcg aacggattgc
     4681 atgacatgaa acagggaggc aattcgcgca cgcgcaggcg cggaacgcga cgagggtcgg
     4741 cgcggtcgtg gggcgcctgc atgatcgtcg cgttgctgct cgggtggacg caggtcggcg
     4801 cgacaacagg ccggggcggg gcaccgggcg agctttcggc cttgctcgca gggaacggaa
     4861 gtgcgcagcg ccatacccgc gcgctgacgc tggcgacagc ggaggcgcag cgtcccgccc
     4921 gccgccatgc gggcgcactg gatgccacgc cctggcttcg ccatgcacag gtcgcggtgg
     4981 cgctcgtgat cctggtgttc atggtggcga ttctctgggg actggtccgc cagtcccgcg
     5041 ggcggtctcg cgccgagcat cgcctccgcg cgatcgccga cgccgtgccg gggacggttt
     5101 acgtattgcg ccgcacgccg cgcgacgtct tcaccttcga attcctcggc gtgaacgccg
     5161 tggaaatcct cggccttgaa cgcgaagagg tgttgcgcga caccaacgcc gcaaggcgcg
     5221 tgatcctgca cgaagaccgc gatcgcctgg cgacggcgct ggcgcgctcc gcggacagca
     5281 tgtcggtact ggaagtggac ttcagggtgc gcaagcccga tgcctccttg cactgggtgc
     5341 attcatcggg gattcctgcc cgccgcgcga acggcgacgt ggtgtggaac ggctatctct
     5401 ccgacatcac gcccatcaag gaaagcgagc aggcgctgca cgatgcgttg cgtcgactcg
     5461 acgatgcgca caccgtggcg agcctgggcg actggacctg cgacctggcg accggcgcag
     5521 tcacgtggtc gccgcagatc taccagatgc tcggacgcga tccggcgctg ggtccgcccg
     5581 acctggccga gtcggtcgcg ctgttcgaga acggcgcgga gacgatctcc gaagccttct
     5641 tccgcgccca ggaaaccggg caacggcagt cctacgagct cactgcgcgg ctggcgaatg
     5701 gcgaggcgct gacgctgcag gtgttcctgc tgccgcaagt ggatgcgagc gggaccatcg
     5761 tgggcatgcg cggcacgatc caggatattt ccagccgcaa ggcgctggaa gaccgcctgt
     5821 accaggccaa ggaggcggcc gacagcgcca acctggcgaa gagcgatttc ctggcgacga
     5881 tgagccacga aatccgcacg ccgttgaacg gcatgctcgg catcctcgaa ctgatcgacc
     5941 agacaccgat cagcccggaa ttgcgcggcg cgctcgacac gatccgcgag tccggccatt
     6001 ccctgcagcg catcatcgac gacatcctcg atttctcgaa ggtggaggcg ggcaagctcg
     6061 agatccgccc ggaacccacc tcgctggtcg acctggccgg cgcgctgcag cgcgcgtacg
     6121 caggcagcgc cagcagccac ggcctggagt tgcgccacaa ggtcgatccg cggatcagcc
     6181 ccgccgtcat ggtcgatgca ctgcgcctca ggcagatcct gggaaatttc gtcagcaacg
     6241 cgatcaagtt cacgccgcgc ggccatgtcg aattgcggat caacctgacc gaacgcgacg
     6301 aaggacgcga gtcgctgtgt ttcgaagtgc aggacaccgg gatcgggatc tcccaggagc
     6361 gccaggaacg cctgttccag cccttcgaac aggcggacat cgacctcgct tcgcgggtga
     6421 gtggcaccgg cctggggctg gccatctcgc gcaggctggc ccggctgatg ggaggcgagg
     6481 tcagcatggc gagcgagccc ggcaagggca ccacgctctt cctgaccctg tcggtgttgc
     6541 ccgcggaccc cgcgaacgtg gcgccagcgc ccgcccttgt cccggcgtac gcaccgccat
     6601 cctcgatggt cgacggcgca ctggaacggt cagcggcgca cgcggccagc ggtgcgccgc
     6661 tcgtgctcgt cgtcgatgac caccccatca accggatggt gatgagcaag cagttgcacg
     6721 cgctcggcta cgcaccggaa gacgtggaaa gcggtgccga agcgctcgaa cgatggacgt
     6781 cggggcggtt cgccctggtg ctgaccgact gcaacatgcc gcgcatgagc ggctacgagc
     6841 tggcgcggca tatccgccat cgcgaatccg cgggagggct cgaccgcacg ccgatcatcg
     6901 cctgcagcgc caacgtgatc ggcggcgcgc gcgaggcctg cctggaggcg ggcatggacg
     6961 actacctccc caagcccacc aagctgatgg cgctcgccga catcatgcat cgctggttgc
     7021 cattgccggc gaaggcggaa gcggcgcctg ccccgccgga agaccttgct gcgacggatg
     7081 cgccggttgc ggacgctccg ctggacatgc gcgtgctgca tgggatctcg aatggcgacc
     7141 aggcgatcga acgacgcgcc ctggcccatt tccgcaggat caacgacacc gattcgaggt
     7201 tgttgctgga ggcggtggaa cgcgtgcaca tggcatcgat catccatctc gcccaccgca
     7261 tcaagggcgc ctgcaagttc gtcggcgcgc acgcgctggc cagcgcgtcg gaatcgctcg
     7321 agcacgcggg gcgaaacgga accacgcgcg acctcgatcg actggtggcg caattccgca
     7381 ccgaactgga gaaactgaat gcctatctcg acgcacgctg atccgctgga cgcgccgcga
     7441 tcccatgggg tgcccgcgag cgaactttgc ttcctggtgg ccgaagacca cgagttccag
     7501 cgcagcatgc tcgtgcagat gctcgagaac ctgggcgcca cgggcgtgta cgaggccgag
     7561 gatggccgcg ccgccatgga gatcacccgg gaactggagc accccttcga catcatcgtc
     7621 accgacatcg acatgcccgg catggacggc ctgtcgttca tcaggcgcct cggggaggcg
     7681 ggcgtcggcg cctcgctgat cgtcaccagc agcctggaca ggcccctgct cgacaccatc
     7741 gagacgatga gcgcggcgta cggcatgcgc ttgctgggcg cggtcgagaa acccctctcg
     7801 caggagcaac tggcggaact gatcgccctg cactggcggg ccaagccgaa tcccgaacgc
     7861 cctcggccgg gagcggcggc ctttaccctg gacgagatcc tggcgggact gcgcgcggac
     7921 gagttcgagc cgttctacca acccaaggtc gaactgcgca ccggtcgggt caagggtgcg
     7981 gaagcgttgg ccaggtggcg gcatccgcgc cacgggatca tcctgccgta tgccttcctg
     8041 ggcctgctgg aagacaacgc gcagatcagc gagctcacct ggtcgatcct ggcccagggc
     8101 gcgcgcgatt gccggtcgtg gcgcgacgcg gggctcgacc tggacgtctc ggtcaacctg
     8161 tccgggagac agctggcgga cccgggcatt tccgatgcca tcacctggca ggtgatcaac
     8221 cagggactcg agccgaagca catgatcctg gagatcaccg aatccgccgc gatcaccgac
     8281 gtgggccgcg tgctggaaaa cctgacgcga ctgcgcatga agggcttcgg gttgtcgatc
     8341 gacgactacg gcaccgggta ttcatcgatg cagcaactga cccgcatccc cttcaccgag
     8401 ttgaagatcg accagtcctt cgtgatgcat gccgcccgcc aggagtccag ccggctgatc
     8461 ctggaatcga gcctggacat ggccaggaag ctgggcatcg tgtcggtcgc cgaaggcgtg
     8521 gagacgcccg acgactggaa cctgctcagg caaagcgatt gcgacctggc gcaaggcttc
     8581 cacatcgcca agccgatgcc gtcgggcgac ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnccggtgct atgctcgcgc cgccgccggg gcgtgggacg gcacggagca
     8761 acacgatgcg catcgcgggc ctgctgctgt gctgttgcgc cgccctgctg gccgcggggt
     8821 gttccggaca ggatgcgccc gcgccgacga aacccgcggc catcgcgccc agcgtcgcgc
     8881 cgcaaccggc catcgaaccg aagatcgtcg tgcagctcgg gcaccagtcg ccggtgctgg
     8941 cggtgcgctg gatcgatgct ggccgccacc tcgcatcgct ggcgcgcgat ggttccatcg
     9001 tgctctggaa cgtggccgac ggcacgatcc tcgaccatgc ccgggtgccg ctcgatccgg
     9061 cgctgttcga gggcgatgaa gccccgctcc gcttccatgc cttcgccgat gcaccggatg
     9121 ccggcaccct tgcgatcacg tatgcgggga ccacggatcg cgacaccgaa cgcgcctgcc
     9181 cggaggcacg ccaacccggc acgcgctggt gcgcctatgc gctcgacctg gcgacgcgca
     9241 tcgtccgcgc ggatcccggc ttgccgctgc cggccgcgag cgcgaacacc accgcttcgt
     9301 actggccgat ctcgccggac gggcggctgc gaccggaacc caaccacgcc aacggccgcc
     9361 gtggcctgtt cgacctgtcc gacgagcaca tcgacttccc cgatccgacc tgcgtgtcgc
     9421 cgcaacgctg ccgctacggc gtcaccctgc tgcccaccgg cggcggggcg ccacgcgccc
     9481 tgaccggcga accgcgcagc tatttcctcg acgccgacct ctcgccggac ggcttgcggc
     9541 tggtgcgcgt ggaaggcctg cagtccgaga cccgcgcgcg ggtcgagacg ctcgacctgg
     9601 ccagcgccaa cggcgaccgc accttcgagc cagcgcgcgc ctaccaccac gcgcgctggc
     9661 tcgatgccac gcattacctg ctcggcagcc acggctacga cgccaccaac gacaccgatg
     9721 cggcgatggc gggattcccg ccggcgctgg tggtcgatgc cgcctgcgca gcgcgcggcg
     9781 actgccccac gatcgacagt cggtggcaga tgcggccgct ggcggatggc ggcttcgtcg
     9841 cgctgggatc gctcgcgtca ggctgtttcg gcagcggtgg ccgtcgggtt gatgacgtgt
     9901 tctgccccgc cgccgagggc cacgccgacg acggcaactt cacctccgat ccaccgccga
     9961 ccggcctcgc atggcgcgca cccggcgcca ggcaatggca gccgatggcg caggcggcgt
    10021 tggcggcgtt ggcgggccag gtcatcactg cgatcgagcc atcgccggac catcggcaac
    10081 tggcggtggc gacgcgcacc tgggaccgcg ccgacgctgc ggatccgaag caggtgctgc
    10141 ggctttggct gctcgacctc gccgatggcg tcgcgacctc accgcgccgg ctggtcgaga
    10201 tcgtggatcc tgcgggcggg cggatcttca ccgacgccgt caccatccgc gccctgtcgt
    10261 tcagcgccga cgcccgccgc atcgtcttca cccatgcagg gtcggcatgg gcggatgccc
    10321 gtgggctcaa ggccgatgcg gacctgtacc tggtcgatac ggacgcgggg gcgcggatcc
    10381 gcaagctgcg gggcttcgcc cggcgcgcgg tcgcgatcgg cgacgaccgc gtgctggggc
    10441 tggatgacgg caccctgctc gacctcgaga gcggccgtgt cgtcgcccgc atcgcgctgc
    10501 cggcgccgct ggtgcgggcc ggctggatcg agcgcagccg cctgttgtgg gccaccaccg
    10561 acgacggcgc gatccacttc ttcgatgccg gaagcggcac gccgcaactc accctgcaga
    10621 ccttccccgg caatcgtttc ttcgcgctgg cccccggcgg ccgctacgac agcaatctcg
    10681 gtgccgacac cgcgctggtg cgctgggtgg tccccgacgc accgtggcag agcctggccg
    10741 cgcaaacctt catgcgcgac tactacgaac ccggcctcta tcgtcgcctg ctggactgcc
    10801 gcgccaacga cagctgcgcg accgcgttca agccgctgcc ggcgatcgcc agcctcaacc
    10861 gcgtgctgcc ggcggtgcgc atcaccggcg tgcgcgcggg ccgcgatgcg gccgaagcgg
    10921 tggtgtcgct ggaagtgcgc gagggcatcg atgcccatgc gccgaacggc aagacccgtt
    10981 ccggcctgta caacccacgg ctgttccgca acggccggct ggtcgcgacg atgccagtgc
    11041 aggtcgatgc cgcggatggc gacctcactg catggcgcag gcgcaatgcg atgcaggcga
    11101 ccaacggggt gaagcgcctc gcgttcaccg tgccggtgcc gacccaggcg ggcgaagccg
    11161 cacaggtgtt cacggcctat gcgttcaacg aggaccgcat caagggcgag accgcgcgct
    11221 tcgcgtggac gcgacccgcg gtggcgacgc ggccacgtcg cgcctatgta ctggcgatcg
    11281 gcatcgacga ctacgacacc ccgcgcttcc gcctgaagta cgcggtcgcc gatgcgcgac
    11341 tcatggccac gcggctgtcg gccattcccg gtcacgacac ccatgtgctg gtgctggccg
    11401 gcgaacgcga cgccagcggc cgccgcaccc gcgtggatcg cgcaacgctg ggccgggtgc
    11461 tgtcgctgct ggccggcacc ggcgaccgcg aggccacgct ggccgcattg cgcgccggcg
    11521 gcatcgaggc cggcatgctg caggcggcca cgcccgacga cgccgtgatc gtcagctatt
    11581 ccggccacgg ctgggcgaat gcgcagggcg acttctatct catcccgggc gatgggcgct
    11641 gggcggacgg cgcggccacg ccggacctgg ccagcgtgtt cgccaccgcg gacctggtga
    11701 cgccattcca ggcgatgcag gccggcgaga tcgcactggt gatcgatgcc tgccattcgg
    11761 cggccagcgt cgccgacggc cgcttcaagc cggggccgat gggcgacagc gggctgggcc
    11821 agctggccta cgacaagggg atccgcatcc ttgccgcgac ccaggccgac gacgttgcgc
    11881 tggaggatgc gcggcttggc cagggcctgc tgacctatgc gctggcggtg gacggactgg
    11941 gcgacggggc tgccgacctc gaccgcgacg gccgcatccg catcgacgag tggctggcct
    12001 acgcggtgcg gcggatgccg gcgctggcga gcgatgcccg cgtcggtcgc atcgccccgg
    12061 gcggcgacgg cgcgcgcggg atcaccttca gcgacctgcc ggcgaatgcg ccggcgcgac
    12121 gcgtgcagca gccagcgctg ttcgatttca atccgcagcc cagcatcgtg gtgctacgcg
    12181 ggaaaacgca gtgaagcgcg ctacgccgtt ggcgtggctg atcctggccg gcttgctggc
    12241 gggctgctcg ccttcgcctg cacccgcgct gccaaacggc gatgcggcat ccacgcgacc
    12301 cgcagcggac acgcgccgcc tgcatgcgct gatcgtcggc atcgaccgct atcgcttctc
    12361 cgatgcacgc cagccgggcg cggtgttttc cgacctgcgc ggggcggtcg gcgatgcact
    12421 gcgtttcaag caggcgctcg cggacgtgtt cggcgtcgat gtcgatgcgc ccgcggccgg
    12481 tgcctgcgag tcctccaacg ccgcgaccac caccctgacc gacgactgcg cgacgcgcgc
    12541 gcggatcctc caggcgctgg aggaacggat cgccacgctg cgccccggcg acaccctgct
    12601 gttctatttc gccggccacg gctcgcgcta ccgcgacgac gaggcgcgcg accaggacac
    12661 tggctacaac ggcacgatcc tgccggccga tgcacgcaat cccgacgggt cgccgggcga
    12721 cctcttcgat gtcgaactga aggcgctgaa ggatcgcgcg accgcgcgcg ggctgtactt
    12781 cgtcagcgtg ttcgatgcct gcaactccgc caccgccacc cgcgacggtg ccggcgggca
    12841 atcgcggagt gcgccgccat tcgcgggcag cgcgccaccc gcgctcacca cgagtgcatc
    12901 cggcggcgac ggctactggg tgcacctggc ggccgcgcag gacggcgagg aggcgcagga
    12961 aaccggcggc gtgggcacgc gcgccggcgt gttcaccacc gcgctggtcg atgccctgcg
    13021 catgccgggc atgcgcgacg ccaccttcgg cgacctgatt caggaggtgc gcctgcgcgt
    13081 cgccgcccgc ggccacgcca cgcagacacc gtcggcggaa ggcgcgttga acgcggcctt
    13141 cggcgcgcgc gcgcgcacga cggtgctgtt cgatgtcgcg caatccgacg gcacgccggt
    13201 gttgcaggcc ggcgcgacca gcggcatgac ccgcggctcg cgcttcgcgc tgtacgccac
    13261 gcaggccgat gcgatggcag ggcgtgcgcg catcgccacc gcgtcgatcc gtcgcgtcga
    13321 tggcgcgact tcgacactga tgctcgacag cgaagtcagc ggcaagttgc cgaagcgact
    13381 ggtcgccgaa caggtcgcgc acttcctgcc cgtggacgca gtgacggttg ccaacgacct
    13441 gcccaccggc ccggcgcgcg atgccgccgc gaaggcggtg caggcgaccg gtttcgccag
    13501 cgtggcggcc acgggcagca cccgcctggt cgcgatggac ggcgatcccg acgccgtcgc
    13561 gctgcgcgcc gacgacggcc tgctgctggc cgaactcggg cgggtggacg atccggcatt
    13621 cgccgatcgc ctggccgagg ccctgcacaa gatcgcccgt gtgcagcaac tgctggcatt
    13681 gcgcacgaac gcatcggcat ccgccagcga ccccttcccg atcgccacct gcatcgccgc
    13741 cgatggctac cgtccgaccg cttgcccgcc cttgcaggcc ggtggcgtgc gccggatcgg
    13801 cccgggcgag cggatcaccg cgaccgtgat caaccgcggc accgtgccgg cgtatgtcta
    13861 cgtgctggca atcgatccac gcaatgcggt cgatgtggtc ctgccgaagc ccggcgagtt
    13921 cgaccggccg ctgccgccca accagcccta ccggcgctcg ggcatgggct tcgatacacc
    13981 cggcgcctat cgtttcgtgg tgctggccag cgccacgccg gtgcgcgcgg atgcgctcgc
    14041 ccaggccggc accggcagcc gcgacctggc cgcctgcacc tcgccactgg aacgattgct
    14101 gtgcgccagc agcgaaggcc gccgcgatgc cggcgttgcc gcggtcggcg actggtcggc
    14161 gacgatctcc agcgtgcttg ccctgcccaa ggagcctgcc ccatgaatcg attgcgcccg
    14221 catgcgtcgt tgctggtttg catcgcgtcg tgcatcggat tcgccgcgtg cgagcgtgcg
    14281 caggcgccct cgccgccggc atccgcaacc gcgaagccgc tggcgaagac cacaacgaac
    14341 gattgcgaag cgcgtcgcgc acgccacgac cagcagcagg ccggacgcat cgtcggtggc
    14401 gaaccggcga aacccggttc cgcaccgtgg caagtcgagt tgctgtcgtc gccgcagtac
    14461 gacgccgcgg atcgcgccta cgacgccacg cttgccgatg acgatgcctg caagatcttc
    14521 ctggcccagc gcaacccgta cgagcttgcg cacaaatgcg gtggcgcgtg gatcggcgac
    14581 ggctgggtgc tgaccgccgc gcactgcgtc gacgacatcc cgggtttcga cggccgcgaa
    14641 ggcaatgtgc tcaccgaccg ccgggtccgc ctgggtacgc agaacctcac cgtggacgat
    14701 gggctgttcg cgatcgatgc ggtggtcatc cacggcgctt acaggaaaaa caccaaacac
    14761 gacgacatcg cactggcgcg ggtggtggtg gatccgcgca tcgccgcgtt cgaagccgcc
    14821 gggcggctgg ctgcggtgcc gttggtggga agcggcgatc gcgatttcga tcccggcgaa
    14881 gccctgcgcg tcaccggctg gggctggatg ggccggcgcg acgtgcgcga tgcggtcagt
    14941 cgccgcgaca gtgcgaaaca gctgcagcgc aatcctgccg acctgcagca ggtgggcctg
    15001 aactacctgc cggacgcact gtgcgtgaag gaatacgggg cgatctacgg gcccggctca
    15061 ctgtgcgcgg gcacgctggc cacggacggc cgcgtcgaaa ccggcaagga cagttgccag
    15121 ggcgacagcg gtgggccgct cacccgcgaa gaggacggcg gcaggcgcag cctggtcggc
    15181 ctggtgtcga acggcaaggg ttgcggcgcc ggcatcccgg cgctgtacac gcgggtctcg
    15241 cattacgacg gctggatcgt cgcggcgaag gccgcggcga gatccggcag ggtcgaacgg
    15301 gtggcgcagc cggccgacgc gaccggatcg cgctgaggcg atccggcgcg acgacgggcg
    15361 cgtggcttac tgttcggccg gcgtggtgcg ctcgacggtg ccggtctgtt cctcgggacc
    15421 ggtgcgctcg accgtcccgg tctgctcttc cggccccgtg cgttcgaccg taccggtctg
    15481 ttcctccggg cctgtgcgtt cgacggtgcc cgtctgcggc tccggatccg tgcgttcgac
    15541 cgtaccggtc tgcggttccg ttgcggtcgc cgctgctgcg ggctcggcgg caggttccgc
    15601 cggcttcttg caggccacaa ggccgaaagc caggccggtc accaggacgg ccgcgatcgt
    15661 attgcgcatg gtgcttcccc ttcgttcccc aacgaatcag atgaagccga cagccttcgc
    15721 cagcgcgagg tagccgtcgg tgcggtgcag cggtgccagc agtggatcag cgtacatcag
    15781 catcatgccg ccgtcgcgca ggtcccaggc cgcctgcagc gannnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15901 nnnnnnnnnn nnnnnnnnnn nngcgtggtc accggcgttg tggcggatgt cgccctgcga
    15961 gcggaacgtg cgcgcgttga gcgggtcgag cgcgaccgcg cgcaggatca cgccggtggc
    16021 caggtcgtgg tcgcgcaggt tggagcggaa ggtggcgtag cggctgagca cgtcggcatc
    16081 gccgggcgcg agcgcatgcg agcggtcgaa cggcacgcgt gcgccgcgca tgtccagctt
    16141 gccgctggcc agggcgaagc ccagcgcgga gaagccttcg gcgaactgcg gtgccagcgt
    16201 ggtcgcggtg cgcgccgcgg cggccgcgtc gtcgtaggca cgcacgcgtt cgtcgtggcc
    16261 ggcatacagg ttgccgatca ccaccagggc gcgcgcacgc gccgcatgcg cgccggcata
    16321 gcgcgcgtcg agcgcgatcg cgcggtcgaa ccagtccagc gcgcggcgat cgctgtcctc
    16381 gtcgatgccg gcctcgtaca gcgcgcggcc atgcaggaac gcatcgaagg cttcgacatt
    16441 cgcggtgccg cccacctgcg cgccggcagt ggcggccttg ccgtcgatcg ccgcggagag
    16501 tgccgtggcg accgcggcgc cgatctccgc ctgcaccgcg aagatgtcgg acagcgggcg
    16561 ctcccaggta ttgacccaac ggctcaggcc ggtggcgcca tcggtgagtt cggcggcgac
    16621 cttcacccgg ccgccggcct tctgcacatt gccgccgagc aggaaatcca cgccgagcgc
    16681 gcgggcgaca tcgcgcgggt cgccctgccc ttcccctgcc ttggtggacg aggtctgccc
    16741 ggcgatctgc agctgcacat tgcgcgacag ctgcgcgcgg atttccgagg tcaggccatc
    16801 ggagaaatag cgttgctcgg gatcgccgct gagattctcg aacggcagca ccgcgatgcg
    16861 acgctcgcca ccggccgcat ccgtgcctcg cgcggccgtg ccggcatcgc gcgcgcgcca
    16921 cagcaacacg ccgctgccgg ccgcgaccac cgccagcccg gcggcgacca cgatgcgccg
    16981 gcgcgtcggc gaggccagct cggcacgcgg cacgggcaac gcgggcgctg gaccttcatg
    17041 cagcgcggcg accgcgcgca gcagcttgcg catcggttcg ctgcctggct tgcccaaggc
    17101 atgcgagacg tcgatcgact ggaattggcc gaagcccagc ggcggcaggc tgccgtccag
    17161 cgacaacggc accagccgcc cgctttcgcg cccgcgcgtg gcctcgtcgt gcacccagtg
    17221 cgatccagcc gaggtcttcg accacagcac caccacggcg cgcgcaccgg ccagcgcggc
    17281 ctcggtcgtg cgcgagaagc gttccccgcc ttccagcaag ccgtcccacc acacgccata
    17341 gccggcggcc tcgagcacgc gcacgatcgc cagcgcgccc ttgcggtcgg cgcgcgagta
    17401 actgaggaac acggtatgcg ttggcgtcgg gccgccaggc tcaccatcga ctgcgggtgg
    17461 cgtcctggtg ctcatgccgg caacgcgatc ggttttccgc tgaacacggc ctgccagctt
    17521 tcgatctcgt cgacgacgat gccgtcgatg ccgtgcgcca gccgggacac gccttcgtaa
    17581 tcgatcgcgc tgtccggccc ggctagcagt tgcgcgatcc gcacttccac ctgcgccagc
    17641 ttgccggcag tgacttcgga gatcgccgcg aagcgctcga agtcgccgaa gtaacggatg
    17701 ccggcgatgc tggcgcccag cgtgggcagc gcgatgccga ggaacgcgaa cgccttcgag
    17761 gtggcgtgcg gcaggtcgcc gggcagcagg tgcaacgccg cgccggcctt catcagcaac
    17821 caggtcgcga ccgacgcgac cgcgatcttg aacagcgttt cggccagccg gtcgaggtgg
    17881 cggtcgacgg tttccaggcg gcgcgccttg gcgtggtggt aggcccgctg gtcggcgacg
    17941 tgcggcgcca gcaggccgtg caacgcggcg cgcaggaacg gccgggtcac cgccatcgcc
    18001 ggcagccccg gtgcgcgcat cgcatggcgc gcggcgtatt ccggccactc ggtgccggtc
    18061 gcacgcggcc agtggctggg cggccgggcc acgccgacca gcagcaggat cggcgcatgc
    18121 cgcaggtatt cggcggcgcg gcgggtctcg aaccagcgcc cgtgcaagcg gcggcggcca
    18181 cccagccagg tgatcgccag gatcgccgca agcagtccga attcggccag ggcgaacagc
    18241 cacttgccgt cgtcgatgcc cagcggcaac cacgccacgc ccacgatcac cgcgagcgcg
    18301 gacagcagga agttggcgac catgccgctg cggtaactgt ccgacaatcg cgccgaaatg
    18361 ccatcggcgc gggcgaacgc cggcaggatg tcgtgggcca ccgcggcgac gaaggccggg
    18421 tcggtgccgg gcagcctgga cgcggcttcc accagcggcg caccgctgcc gcgggcgatg
    18481 cgctccggcg cttcgtaggc ctgcaccagc gaacgcaacg gatgcgcttc gccgccgaac
    18541 agggcctcga tccgccggta cagggtggac gcgcgcgtgc cgcgtgggtg ccaggcctcg
    18601 tcgcccagcg agcgggccac gccgcgtgcg cgcagcgcgt tgccgacgac ggcctgcagg
    18661 cgctcgcgat cctggccctg ctggtcctgc cagccggcca ggctctcggt cgacgacagg
    18721 attgaccagt ggcggggtcg cgccgggtcg atcagcagca ccggcgtgcc cagttcgagc
    18781 gcacgcacga tggtgtggcc ggtgccgcca acggaatcgc ggctgccgtt gttccacacg
    18841 ccgaccagca ggtccgactg ctcgacgatg atccgcccgg acagtgccgc ctgcgccgcc
    18901 accgccgcgt ggaaatgccg cgcttgcgtg gcatcgtccg gcgacgccag cgtggcctcg
    18961 aacagcgcgg cgatctcggc gtcgcggtcg gccagttgga acagccgggc ccgcgcgctc
    19021 cagcggcgga tgtcctgcgc acgcgcctgc acgtcggcat cagcggcgtc gcctccggcg
    19081 agcagggcca cggcatcggc gtgggtgcgc ggctgcgcgt tgatcgcgac gttgagcgcc
    19141 tgtccgaacg gcagcggcgc gaccagctcc cagcccagcg cgtgcgccgc cgaggccgcc
    19201 agctggtcgg cgccggcgga gaggagggtg tgcaagcgcg tcggctgcgg ttccagcagc
    19261 ggcgtggcga cccgttcggc ggcaatggcc gattcgatct ccgcgaagat ccccgcaagc
    19321 gtcgccgcga cctgcgttgc attggcagcc agcgccgggt tcgacgcgcg gtggccggtg
    19381 atgccgatgg cgaagcgcac cgactgtccg gcgggcgtcc ggctcatgcg cctgcctgca
    19441 ggcggacgac ggaaacgcag gcgatggtgg cggatcgggc acgtggcttg cgcatgctgc
    19501 ttgcccgtca ggcctcgtga ggtcgcggcg agcatagcac ccggtccgcg cggcccaggg
    19561 gctcgatggc atgcatgcgg agcgggcaaa tcctggatca acgacgcatc gtgcgacgcg
    19621 cgtgccggtc ggggacgagc tggagcgggc gaccgagggc gcggcgttgg gcaaccgtgc
    19681 ccaacgccgc gcgaaccgtc atcgacggcg tatcggtgga acgatcagag cgccgcgtcc
    19741 ttgagcttct tcagcggacg caccttcagc ttggtcgatg ccggcttggc tgcgaaccag
    19801 acttcttcct tggtgaaggg gttgatgccc ttgcgcttcg gcttggccgc gaccttcacc
    19861 gaagtgatct tgagcaggcc cggcagcacg aactcgccgg cgcccttctt gctgacggaa
    19921 ccgtggatcg cgccttccag cgcggccatc acggcgcgca cgtccttggc cgcaacctcg
    19981 ctgacttccg cgatgtgcgc caccagaccc gacttggtca tggcttcctt gatcggtttc
    20041 ggtgcagcgg gtttcgctgc ggctttcttg gccggggccg ctttcttcgt tgccatttcg
    20101 atttctccca gtggttggca ggacagcagg cggattggag caccctctgg ccgatctgtc
    20161 atgtggcagg ccgggtggga cttggtggtt gctgccttca gggacgggag aagcgcgacg
    20221 tcgggcctgg ccaggcatcc gcccttgtct gacggcgatg acgagagcat cggggctgtc
    20281 gatacccatg ccgggctccg gtgccccgaa ccgggaaccc acgcgacgca ttgccggcgc
    20341 gaatgcgtgc gcccgtcagc aacaacccgg cgcgatggac atctgctttc ctgcattgcg
    20401 cgtctgccgc ttcttcaagg caagacaagg ccgccccgag aacgccgcgc aacccgaacc
    20461 tcgcgacccg gggcccggtt gcaacgatcg gcaaggcggc tccctgcagc tggcctcact
    20521 cgaacgccca agtcaacgct cggtgcggcg accgcggagg tcgagggcgc cgcacttgtt
    20581 aaggaattgt gcagaaccta tagtccgcca gtcatggccg attcaggtca ggcgcagggg
    20641 cgttcttttc cgattatttc tcatggggat ccacaacaat ggcttctaag aatttcgcca
    20701 agggcttgaa gcggagcgca ctgaccgtcg cgcttggcat gtgcttcgcc ggcggcgtcc
    20761 aggcacaaag cacgtccggc gccatctacg gcaaggccga tgccggccag accgtcacca
    20821 tcgtcagcga aaccgggttg acccgcaccg tcaccgcgga tgccaacggc cgctacagcg
    20881 caccctccct gcccgcgggt cgctacaagg tcacggccgg cggcgacgcc cgcgatgtca
    20941 ccgtgctggt ctcctccggc gcccaggtcg acttcggcga cgccacgacc acgctcggca
    21001 ccgtgacggt gaatgccagc tcggttccgg tgatcgacgt gtcccagacc gacagccgca
    21061 cggtgttcac cgccgagcag ctccaggaaa tcgcggtggc gcgcgacatc aacgccgtgg
    21121 ccctgctggc gcctggcgtg atcaattcca ccgcctacaa cagccgttcc tccaacgtgg
    21181 gcagcttcgg tggcgcagcc gcgtccgaga acgcctacta catcaacggt ttcccggtca
    21241 ccaatccgct caccaacatc ggttccacca ccctgggctt cgattccatt gcccagcaac
    21301 aggtgctgct gggtggctac ggtgccgaat tcgggcgcag cacgggtggc gtgatcagca
    21361 tcatcaccaa gcgcggaacc aacgactgga agggcggtgt gtacgccatt tacacgcctg
    21421 catcgctgcg aagcgatttc aaggacatct actatccgga cacgggcttc tggaacgcga
    21481 caactcacta caacaccact gccggcaaca atccggcgaa ctggaccgat ggccagttgt
    21541 atgacctcaa ccgctacaac aagtccggaa cccaggtcta cggcttctac atcggcggcc
    21601 cgatcatcaa ggaccggttg ttcatctatg cgaacgccga gcggtcaaag accgacagtg
    21661 aatcttcccg ctattccgcc aacctgcttg ggaagactgc tgcgggcctg ccggcactca
    21721 gcgccacgag ccgcgcgcaa gcatggggcg tcaacgaatc caccgacccg cgctggacgg
    21781 tcaagctcga ctggaacatc aacgacgacc acatcatcga atttaccggt gtgcaagaca
    21841 acaacaagtt cgagtccgag tactacggct acaactacga caccaacgag cgcgacaatg
    21901 tcctgttcaa caccaacaag acggaatcga agacacgcct gtacatcggc aagtacaccg
    21961 gctacctgac cgacaacctg agcgtttcgg ccatgttcgg ccaacagaag atcgacaccc
    22021 tggccaaccc gctggacggc tacgatccga gcaagacgta cgtcagcatt tccccaacgg
    22081 tggtgcccac ggcgtttgcc ggcatcagca acccgcagaa gttcgggccg acctacaact
    22141 acgacggtct ggacgaaacc aaggcacgcc gtttcgacat caactactcc atcggcgacc
    22201 acgagttgcg cgcgggttac gactacttcg aggccttctc ctaccgcggc gataacgtcg
    22261 tgggtccgac cggctactac tggtcctacg gccttgccag caacccgaac gccgcgcttg
    22321 atgccagcca ctatgtcggc tcacctgctt ccggtggcgg tctgggtgcc aacggctact
    22381 acgtaacaaa gaactattcc gagcacggtg gcggcacgac cgtcaagcag aaggcgctgt
    22441 tcatcgaaga ccgctggcac atcaccgaca acatcctgtt gagcctgggg ctgcgcaacg
    22501 acggcttcac caactacaac ggcaatggcg aagcctatgt ggagcagaag aacaactggg
    22561 cgcctcgcat cggcttcagc tgggacgtga atggcgattc ttcgttcaag gtattcggca
    22621 acgtcggtcg ctaccacctg gccctgccga acaacgtgtc ggtgcgcggc tccaacccgt
    22681 cgctgagctc cgcccagtac ttcacctata ccggcatcgc ggccgacggc acgccgaccg
    22741 gcctgaccgc cattccgtac accaatgcca acagcccgta cacctgcgcc aatggcggca
    22801 tgtcgtccaa cgtggaatgc ggctacccga aggatccacg cacgatcgcc cagatcgacc
    22861 tgaagccgca ttaccaggac gagttcctcg tcggcatgga gcgaaccgaa accgactcgt
    22921 tctcatgggg tgccaaggcc acctatcgcg acctcaagaa cgccatcgac gacatctgcc
    22981 ccaccgaatg cttcatcttc aacgcgggca cgaacgtcgc gaccttctat gaagacgacg
    23041 gcaccggcaa cctggtggcg gtgacgcacg acttcgcggt cgatttcggc accgcgttcc
    23101 cgaagctgaa gcgcaagtac ctggcgttgg acatgtacgc gcagtaccgc aagggcgact
    23161 tcttcggcaa gatcgactac accctgtcgc acaactgggg taacgcggaa ggccagctga
    23221 actcttcggt cgacaccggt gcgggcggcc agggcgatgt ctcggtgacc caggactggg
    23281 acctgccgga actgatgttc ggcgtgaacg gctcgctgcc gaacaaccgc acgcaccaga
    23341 tcaagatgat cggcacctac cgcttctcgg aggagtggcg cgtgggtggt tccgccatcg
    23401 tgcagagcgg ccgtccgcgc agctgctaca gctactggcc gtacgcgaag ccgggcctgt
    23461 acaacaacgc ctactacagc tactgcggcg tgccgggtgc gcaaaccgcg gtcaacaatc
    23521 ccaacgtgac cccgaatgcg gactatgtgt tctcgccgcg cggcagcgtg ggcgagacgc
    23581 cgtggacggc gacgttcaat gccaacgtgt cctacacgcc caactggctg gacggcctga
    23641 ccctttccat ggatgtgctg aacctgctca acaagcagac cgcgaccggc tactacgagc
    23701 gttcggccag cagccgcacc accgtgaatc cgcgctacgg ccaggagctg tacttctccg
    23761 caccgcgcag cttccgcttc tcggttcgct acgacttctg atccacggat cgaagtcgca
    23821 tgatgtacgt cgaccggccc cgcgaggggc cggttttttt tgcgcatcga tgcaggcgcg
    23881 acatgcctgg cggggccagg ccgtcagaac ccgtagcgca ggaacccgcc gtccaccgcg
    23941 atgcattcgc cggtgatgta gctcgacgcc ggcatgcaca ggaacgccac cgccgccgcc
    24001 acttcctcgg gttcgccgac gcggccgagc ggcgtgcgca gcaggacttc gtcgaggtag
    24061 tcgggatcgg ccagcttgct gctggtgcgg cgcgtgcgga tgtaccacgg cgccaccgaa
    24121 ttcacccgaa ccccgtcttc cgcccactcc accgccaggt tgcgggtcat ctggtgcagc
    24181 gcggccttgc tcatgccgta gggcgcgccg ctgcgcacat gggtgatgcc ggacacgctg
    24241 ccgacgttga cgatcgccga tgtcgcatgc ttcgccagca acggatacgc gccgcggcag
    24301 agttcgaacg cggagaacag gttggtctcg aagatgccgc gccactggtc ttcgccgtag
    24361 tcgagcaccg gccgggtgac gttgccgccg gcattgttga ccagcaggtg caggccgccc
    24421 tggtcgctgg cccagtccag gatctcggcg cgctgttctt cgtcggtgac atcggccgga
    24481 agcgccaaca ccgcgtcctc ggggccgggg aactcgtctt ccagttcggc gcgcgcctgc
    24541 tcgagcatgt cggcgtcgcg cgcgaccatc agcacctgcg cgccgaggcc cagcagctcg
    24601 cggcagatgg ccaggccgat gccggcactg gcgccggtca ccagcgcgcg ttgtccgtcc
    24661 agggtccagc ggtgcgatgc catgcgcggc tccgtgtgtc ggggtcgggc gtaggatagg
    24721 ccacgtccga accgctggag cctgtcatgc gcatcgtgtc gccacttgcc tgcctgctcg
    24781 tcatcgcgct ggcaggatgc tcgccgccga agccgccgga agaggagcgt cgccccgaac
    24841 cccaggccgc cgcggtcgtg caggcagccg atgcctacaa ggacagcgcg cgcgctgcgg
    24901 tggcgaagac cgaggacgcg gccgcgaagg agaaggcggc gctcgacgcc gcgacgcagt
    24961 gagtcgctag tccgcggtct tcggataggc gagcaggatc accaggtcgt cgtcgccgtc
    25021 ctgcatcagc gaatgggtac tgccggtgcg ggtgagcatg gcgtcgcccg ggccaacgtc
    25081 acggatgctg ccatcgatga tatagcggcc cttgccgctg acgacgtaat agacctcgtc
    25141 cttgtcgtgc tggtgcaggc cgatgccggc acccttgtgc agcacgcgct tgcggaagaa
    25201 gaacgacagg ccgggcgcgt cggcgaagaa cggatacgcg gtggtggtgc cggcgccgcc
    25261 atgcgggccg ggctgctggc gggccaggtc agcttcgcgt accacctgcg agggatgcgc
    25321 ttgggctgcg cgatcggcca gcgcggtatc gcgcgcaccg cagtcggtat cgcgcaccac
    25381 ggtttttgag atcgcgggat cgagggcttt ccagccgccc acgaccaggc aggccacggc
    25441 ggtcgcaccg cgttgctgca agtgggtgtc gtccgtgagg tcctgctcgg gcacgtgcat
    25501 gtagaacggc ttcgaggctt catcgcccag cgcacgcaac catgccgtgc tcagtgcatc
    25561 caggtcgatc atgccgacct gctcgcgcct ggccaattcg cgcaccgcga tcgaatacgc
    25621 accatgcgtg tccagcaact ggtcgatctt gctgcc
//
LOCUS       DAOOCX010000024        24618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1704_length_24618_cov_8.701462, whole genome shotgun sequence.
ACCESSION   DAOOCX010000024 DAOOCX010000000
VERSION     DAOOCX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 24618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24618
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1704_length_24618_cov_8.701462"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..447
                     /gene="rsfS"
                     /locus_tag="PK399_03840"
     CDS             43..447
                     /gene="rsfS"
                     /locus_tag="PK399_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPW12286.1"
                     /translation="MTTTAHVIKTSLPTPPPSVETLLKTVHAALEELKAKDTVEIDVR
                     GKSSVCDYMVVASGTSTRHVKSIADEVVRFAKKLDVMPLGVEGEREAEWVLVDLGDVV
                     VHVMLPRVREFYALERLWTVGDQHPDYVASDA"
     gene            496..966
                     /gene="rlmH"
                     /locus_tag="PK399_03845"
     CDS             496..966
                     /gene="rlmH"
                     /locus_tag="PK399_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPW12287.1"
                     /translation="MKARLVAVGERAPAWVAQGFAEYQKRLSHWLPLELVEVEPGVRG
                     KGRDTARAMSDEGARVLAALPKNAFVVALDGRGKPHSSEQLAVRLEHWRGRGQDLAFL
                     VGGPEGHAPEVIARADETWSLGPLTLPHMLVRLVLAEQVYRAAALLANHPYHRA"
     gene            complement(1021..1995)
                     /locus_tag="PK399_03850"
     CDS             complement(1021..1995)
                     /locus_tag="PK399_03850"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW12288.1"
                     /translation="MGWNTIPIPRPESPTARPRCPPCAGIVVSGAKNMNESHCHLSAA
                     DCRSSPADCQCAGVRRSVEACTGRASTPSPPLVRCMAYPPDRRLTMARSHSIASFRPA
                     GFALLKPASKPQLDTARIITMSGTIAVNVLAMGLLMMPMTMPAPLPLPDETPDTRLPD
                     WIRRDPPPPIKVPVVQQQPTPPTTQPVRQQTITQQPTAVATPVLTDGATEQAIESTVV
                     DAGPVGDIAPIATGPAPIQLEYKSAPAPDYPRMAKQRGLTGTVLLQVLVGIDGRPLEV
                     TVAQSSGHRELDEAARAQVMKRWSFQPARKDGQAVQAIGMVPIEFALR"
     gene            complement(1961..2143)
                     /locus_tag="PK399_03855"
     CDS             complement(1961..2143)
                     /locus_tag="PK399_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12289.1"
                     /translation="MTTRKDSDAASVPTASPDPACNTGYAEPKPKDKAQAQIPGARPE
                     PDAEDGGLEHDPDPTP"
     gene            complement(2145..2873)
                     /locus_tag="PK399_03860"
     CDS             complement(2145..2873)
                     /locus_tag="PK399_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPW12290.1"
                     /translation="MILRPLFLALSLAIALPMTANAQTPADIAIASPQGTLLSVSASA
                     DATRVPDVATISTGVVTQATDANAAMRANAVQMDKVMAAIRAAGIAERDIQTAGINLN
                     PQYKYVENQPPSIVGYQASNTVNVKVRDLSKLGKVLDAFVANGANQINGPSFEVDKPD
                     AAYDEARLGALKKAQARADTYAAALGMKVRRIVSISEGGASFPRPMPMMRAMASDGFA
                     KETSVSPGEASLSVSIDVVFELGR"
     gene            3018..3587
                     /locus_tag="PK399_03865"
     CDS             3018..3587
                     /locus_tag="PK399_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HPW12291.1"
                     /translation="MLHLASRSPRRAELLARLGLDFGIIDLDLPERQGAGEAAAEYVR
                     RVAREKAGAGLLQVVAVPGAVVLGADTEVILDGRVFGKPADAGDAAAMLRTLSGRTHE
                     VITAVSLVSAAREAQAVSASQVTVAALDDAAIAAYLATNEWQGKAGAYAIQGRFESHI
                     QHLSGSFSGVMGLPMFETARLLREFGIAA"
     gene            3600..5096
                     /gene="rng"
                     /locus_tag="PK399_03870"
     CDS             3600..5096
                     /gene="rng"
                     /locus_tag="PK399_03870"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002802933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HPW12292.1"
                     /translation="MSEELLVNVTPRETRVAVVENGMLQELHIERGSQRGVVGNIYKG
                     KVQRVMPGMQAAFVDIGLDRAAFLHANDIFRSAQLDAGEVDDAPGAPPSMQVQVPITE
                     LLRDGAEIVVQVVKDPIGSKGARLTTQISIPSRYMVLLPRSRVLGISARIEDDEERAR
                     LKALMTELARPGGAYGYIVRTNAEGQPAEALAEDIAYLKRAWDLVEATSATAKVGSCV
                     YEDLSLPLRAVRDLIRRDVDKVKVDSRETCERLREFAAQYMPGLAEKIEHYSGARPIF
                     DMYGVEDEIQHALEKEVPLKSGGYLVIDQTEAMTTVDVNTGSFLGQRNLEETVYRTNL
                     EAAQSVARQLRLRNLGGIIIIDFIDMVDAEHRRQVLRTLEKSLARDHAKTTVYDFSPL
                     GLVEMTRKRTVESLERQLSEPCHECSGRGTVKTAETVTYEVFREIVRAVRQFDAPRLL
                     VIASPKVVARITDEESTAVAELEEFLGKSIRFQADAQYMQEQFDVVLL"
     gene            5214..9086
                     /locus_tag="PK399_03875"
     CDS             5214..9086
                     /locus_tag="PK399_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HPW12293.1"
                     /translation="MTTPVRRRWRLLRRGAWYALAAALVLLALGNGIGSQLLPLVERH
                     PDKVATWLGTRVKSPVAFDHVETEWTRRGPLLRLDNLRIGDAANPLRIGDAEILVAQY
                     AGLLPGRSFTELRVRGLQLTLQRDANGLWSVRGLPGQQQAGGDPFATLERLGELQLSH
                     ARLRVSAPELGLDLRVPRVDLRMQVDGGRIRVGAHAWLRAGATPLSAALDYDRGSGDG
                     RVYAGTRRADLGEFAGSFDLAGVVPVSGGGRLRAWGQLQAHRIVGIRADAALQDVVVR
                     GAPSADGQPPPTQALGKVALDARWSGTVRQWQAKATRLRLGSGDAEQVMDGLVVRGGQ
                     RYGLQARRIDAAPLLQLLALGDVLPLGMRGWLQASSAGAVLEDVDVEGVRGGVVSATA
                     RIRGLHFAPVGNAPGMRGVDGWLQGDADGVRLRFDPQAVFAFDWPAGFGVVHQFTADG
                     EAVAWRDGNGWSVRTAGLALVNPDLQVKVRGGMGFANDGTRPRLDLAADLGTAQVSLA
                     HGFWVHHLMPKSTVDWLDAALKGGSLHDIHAVVAGDLDDWPFRTEGGRQGAGVFRVDA
                     RMQGGVLKFQPDWPAAEDLDADIRFEADGFTVAGRSRIAGVPVAALKAGIARFGRAEL
                     TVDAAAAGDAKDFLAMLRASPLQKSYGASMDSLRVAGPAQAQFHMLLPFHHDKPPPHD
                     VHGDVVLSGASLRDERWKLAFEQVRGNIRYDNAGFAAEGMQVRHEGTPGVLALRAGPH
                     VRDRAHAFEGELDAQIDIDGLLDKAGSLAWLKPHVSGSSAWTVTLGIPRNAAQGAMAG
                     RLQLRSGLVGTALDLPEPLRKPRGQPLVASVDLRLPLEEGEVEVALGDLLSLRSRSRG
                     SATGVAVQLGGARADAPPADGLVVGGRAARVDALDWIGVIAGDRGGDGLPLRRIDVNA
                     AQLRLLGTQFADARVQVVPAPRGTAVVVQGASLAGSLLVPSQEGATVAGRFDRLHWAM
                     PARSGGLLAGVQPPAANVPAATPAAAGAEVPAFDPGKLPPLLFDVGDLRIGEAVLGQA
                     RFRSTPVAGGLRMDEFTTRGGTQRLTATGTWSGRGVAARTRLALGVDSDDIGALLAGL
                     SLGGQVGGGKGKLQVDADWRGGPDALDPLTLQANLRLDARDGRLLELEPGAGRVLGLL
                     GIGQLPRRLTLDFRDFFDKGFAFDSITGDVRLANGSARSDNIAIKGPAADILVRGSAD
                     LRSQRFDQTVDVLPKSGGLLTAVGALAGGPVGAAVGAVANAVLEKPMQGIGAKTYRVT
                     GPWAAPKVEVSARRDPPKTQVAAPDAPG"
     gene            9138..10577
                     /gene="tldD"
                     /locus_tag="PK399_03880"
     CDS             9138..10577
                     /gene="tldD"
                     /locus_tag="PK399_03880"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HPW12294.1"
                     /translation="MNALAIAESRLLAPAGLDTDALQRAFASLLGPGIDFGDLYFQHA
                     RRESWSMEDGIVKDGAHAIEQGVGVRAISGEKTGFAYSDDLDAAALIASAQSARAIAR
                     DGRQHAGQALARSNGRQLYPALDPVDAFANEAKVALLRRVDALVRTLDPRVQQVMVSL
                     SGGVDTVLVARSDGVLAGDVRPLVRMNIQVIVEQAGRRESGFAGFGGRYSYAHLLADD
                     KPETFAREALRQALVNLDAIDAPAGTMPVVLGHGWPGVLLHEAVGHGLEGDFNRKGTS
                     TYAGRIGQRVAAKGVTIVDDGTLDGRRGSLNIDDEGTPTSCTTLIEDGILVGYMQDTL
                     NARLMGMAPTGNGRRESFAHLTMPRMTNTYMRAGTHEPEEMIRSVKRGLYAVNFGGGQ
                     VDITSGKYVFTATEAYLIEDGQVTAPVKGATLIGNGPETMQRVAMIGNDMALDDGVGI
                     CGKDGQSVPVGVGQPSLLISELTVGGTQA"
     gene            complement(10580..11143)
                     /gene="yjgA"
                     /locus_tag="PK399_03885"
     CDS             complement(10580..11143)
                     /gene="yjgA"
                     /locus_tag="PK399_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HPW12295.1"
                     /translation="MRGRDEDTGEFLSPSRSQNRRDALDVLALGETLTALSAAQLARL
                     PIPEELLPHIRETQRMTSHGARKRQHAFLAKQMRRQDDAVLDAIRDALAKDGDAGKRE
                     TAALHRAEAVRDGLMGDDGDVAMTEFLAAHPDADRQKLRQLVRNAHEERKRNKPPRAY
                     RELFREVRELLATDGDEVADPLEDEDA"
     gene            11161..12531
                     /gene="pmbA"
                     /locus_tag="PK399_03890"
     CDS             11161..12531
                     /gene="pmbA"
                     /locus_tag="PK399_03890"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HPW12296.1"
                     /translation="MNSPAIEIAPVADDSRERMQRLSGIAEQLLAQARAAGASQAEVS
                     CNEDSGLAVNVRMGEVETVEATRDRGIAVTVYFGTRKGSASTADLREDSLQATVSQAC
                     AIARHTEDDPASGLADAGLMATTLREFDGWHPANFDADRAVAIALAAEAAGREVDARI
                     GNSDGASANAGATISVYANSHGFLGAERSTSYSIGCALIAGEGEDMQRDSWYSVALAE
                     GDLEPPQAVGRKAAQRTLERLDPRSLPTGEVPVLFAAEMARSLVGHYLGAVSGGALYR
                     KASFLLDSVGTRVFPDWFDIEERPFLPRGFRSAAFDAEGVATRESPLVRDGIVQRYVL
                     GSYSARKLGLQTTANAGGVHNLQVRANAGGLQELVQGMGRGLLVTELMGQGVNPITGD
                     YSRGAAGFWVEGGRIAYPVDGVTIAGNLKQVFAAIEAVGNDVDPRSHIATGSMLVGRM
                     TVAGTG"
     gene            12676..13062
                     /locus_tag="PK399_03895"
     CDS             12676..13062
                     /locus_tag="PK399_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPW12297.1"
                     /translation="MDINEATAPPPVPSGAPSSDDRQWGMFAHLSALSGVVIPFGNII
                     GPLVVWQVKKDTLPFAADQGKEALNFNITMLIAAFIGFLLTFILIGLVLLPLIGIAWL
                     VFTILAGIKANEGVAYRYPYALRLIK"
     gene            13212..13640
                     /locus_tag="PK399_03900"
     CDS             13212..13640
                     /locus_tag="PK399_03900"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPW12298.1"
                     /translation="METSTYESGGAATAVQTLSPSLTDRQWAAAAHAAALVAAMATSL
                     WAGVAGAIAAVGVWMLVRDRSAFASEHAKEAVNFNLSMFIYAAAAVVFSIFTLGIGLI
                     VALPLWGILALVWIVCTLVAAFKAYDGQPYRYPVTIRLFK"
     gene            complement(13730..15085)
                     /locus_tag="PK399_03905"
     CDS             complement(13730..15085)
                     /locus_tag="PK399_03905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPW12299.1"
                     /translation="MTRNTMRWSAAVLAAMVSGLLLAADPITPATVAKPSIEAHAKPD
                     FASPTVATFSKNAPVKVSGQQGLWFQLALDGGKTGYVRVNDVRVAYGKAETGGAAQAL
                     FTGKASKGRVSETASVRGIDESDLKAASFNAAQLKLMESYRATPEAAEQAAAKRGWTA
                     TQVAYAIEYQPGKAKGSGKGGATQAEKRERFGMARGLLSSVGGRLGGGVGATAAASGD
                     KLIGKSEDEVAQEELELGPLIAGRVLGAAPLVKDPAMQKRVNLIGRWLASRSSRPELP
                     WTFGVIDDGEINAFAAPGGYILITRGLYQLLGSDDEVAAVLGHELSHVVQRDHYEVLR
                     KQELQGAGRDVALSRVNTGGGVAGSLARGYIERNGAAIMLTQLDRNAEYRADQAAGFY
                     LARAGANPLVYYAVLQKMTALGSSSQRLTQLYKTHPPLDKRLDALDKQGYKGLEPYLD
                     R"
     gene            complement(15082..16971)
                     /locus_tag="PK399_03910"
     CDS             complement(15082..16971)
                     /locus_tag="PK399_03910"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HPW12300.1"
                     /translation="MARRAFKLLQDRIDATMASLAVWFMDAWTVVERGWRRPLSKLAL
                     RLKFAFYPLLAIAALGWLGWDWTHGRSLDAAEDSLFDKVVKLRPVEPKPSGKTVVVEI
                     DECSIEWTRAQGLGGWPWPRSVHADLLDALDRAGVKAVGVDVMFLDRDPDDPNGDGML
                     DLVAAGGEGRFLFAATFPEDADFNAKAAIRAADAPGAYPLSPEARPPGPPIPLQLPYG
                     DAMAKHAASTNFGRSDDSIVRDVPLRDEVEGWAIPTLPVLLAAQVQGRSPSSYPAQVR
                     VNWRKRTQLPYISAADLLAGETVCHAAGAKRPVLRDAVALVGYSAAGISDTKPTPIKA
                     AMPGVEVWAEATEALLQDSAIWMPPTSFKYLLAALLVLLTSYAFWRGEPHEDVDGVFV
                     ATNATLLLIAFVGLTFFGVFVDIFASVGFVSLCFGLCRLYASVQRGRAIGNNDYRDEY
                     NPQHHPWLLMARLRFVPNGDIEAFAAIRGKREYRRLLRRQLYAGSDAVMIEGIVERKS
                     WLHDILDDLMLLIWTGTERTQALDAAKRELDQLYRDLNEGDLRLEDHGRVMVCISVAE
                     IDDDNDLTNRGERMRLRELLGQDLNATDEWLLVADNRYILDPSHTLEFSDEATDATDA
                     EGEDR"
     gene            complement(16981..17916)
                     /locus_tag="PK399_03915"
     CDS             complement(16981..17916)
                     /locus_tag="PK399_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPW12301.1"
                     /translation="MPDAMFAAWRTRVEAALDRALPDPATSPERLHAAMRHGVLNGGK
                     RMRPMLVYATGRAFGATEADLDAAAVAVELIHCYSLVHDDLPAMDDDALRRGQPTVHV
                     AFDEATAILAGDALQARAFEVLANAPQPAERRVAMLAELARASGVAGMCGGQALDIDA
                     TGKWGRGNWGQSRLSSAKGDSDPTFLLADLERLHAMKTGALLRAAVRLGAIAAGAEDA
                     SRGALDRYADALGLAFQVRDDLLDVEGDAATLGKTAGKDAAQDKATFPALLGIDASRA
                     RLASLADTMQHALSSLAVDTTALAALARLVVERDR"
     gene            complement(17909..18172)
                     /locus_tag="PK399_03920"
     CDS             complement(17909..18172)
                     /locus_tag="PK399_03920"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPW12302.1"
                     /translation="MARKTTPPQDTSPVADFEQSMQSLEDLVARMESGEMTLEESLAA
                     YERGVGLYRHCQSALEQAELRVRLLSDPANPDDARPFDASEDA"
     gene            complement(18182..19474)
                     /gene="tilS"
                     /locus_tag="PK399_03925"
     CDS             complement(18182..19474)
                     /gene="tilS"
                     /locus_tag="PK399_03925"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPW12303.1"
                     /translation="MHAPLADVVRRDDGRGVMLGFSGGLDSTVLLHLLAANTDIRRNG
                     LGAIHVHHGLHADADAWAAQCQRSCDALDVPLSIVHVDVDRASGQGLEGAARDARHRA
                     FAQALADDEILALAHHRDDQAETFLLRALRGSGVDGLAAMRSWRAYANGWLWRPLLDV
                     AREELHEHATAHGLAWIEDAGNGDPRFDRNFLRNAVMPLLRQRWPQASHNLARCAALN
                     AQAADLLEADDVSALQGVMRDDHTLDVDALRAIVPERRARVLRRWIGGLGLQPLPGNG
                     IAVIEADLLPARADTQACFAWSGARIRRWRHLLHAEAMRDPLPAQWSQAWDGTTPLLL
                     PTGDTLSLLGAERFDPPLRVHARQGGERIVLPGRGHSHALKHVLQDAGIPPWRRERLP
                     LLSSNDGTLLAAGDAILSASLAAWLGTRGARLHWARLA"
     assembly_gap    19510..19609
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19610..20259)
                     /gene="accA"
                     /locus_tag="PK399_03930"
     CDS             complement(<19610..20259)
                     /gene="accA"
                     /locus_tag="PK399_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HPW12304.1"
                     /translation="MNPNYLDFEQPIADLEAKIQELRHASNGPAVNVDAEVHTLKEKL
                     RKRTATIFRDLTPWQVSQLARHPARPYTLDYLRVICDEFEELAGDRAFADDNAIVGGL
                     ARIDGRPVMVIGHEKGRDTKAKVRRNFGMPKPEGYRKALRLMKMAERFGLPILTFIDT
                     AGAWPGIDAEERGQSEAIARNLLEMAELKVPIICTVIGEGGSGGALAIGVGDRTLML"
     gene            20361..22757
                     /locus_tag="PK399_03935"
     CDS             20361..22757
                     /locus_tag="PK399_03935"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPW12305.1"
                     /translation="MDPMPMPAVPPASSADVRVPWHASLRTRLMAWVGLLLALLLLAG
                     FATAFLAARERVLADAEARTRYEARQAADRLDATMRSVRVSGEAMIELNNRVALTRAE
                     LLEAMEAMLDANPVTVGGLVALEPGVLADRAPLAFYVGIAARGVPDRDLLADGYDLAG
                     REWYQRTLQATSPWWSEPYFNETAGGRYMTTLNLPLRDRSARRIGMVSLDVPVQALSS
                     SLESLRTVTGQRTALFAPEGTIAVHPERGIAFAHDLPGYIARAGRSDLAPMEAARVQQ
                     RPLQFTHADARDGTVRYSVLQPVGGSGWTLQLSMGRDAMLAGLDSAMRMLGLIGGLVA
                     LLAALAVLRLARRITVPLTELTTSAGHFANGEFDWPVPHDARGDEVGVMARALERARD
                     SIRQQLDEIGRYATERQKLQSELDIARGIQMSMLPRDRDFAVGDIRYRLQARLEPAKA
                     VGGDFHGHFLQGADRVWFVVGDVSDKGVPAALFMARVVTVLEVATAASDAPDRVLADA
                     ARHLSENNDACMFATVLCGVLDLASGELAIASAGHDAPLLRHADGRIDSIPLESGPAL
                     GFDAVDGFEVWRGRLQPGDLLFAWTDGVTEAFDGDDLAFGEERMQAALRTAGSAAEAC
                     DGMLIAVHAFAAGVAQSDDITVFAIDCGSQPVPAAWSMVLDVPGGRDRLPEVLAGVEA
                     HLHAAGIDSGRVHDAQVIVEELACNVMDHGAQAGVDAMRLDVTAADGRVVLELRDNGA
                     AYDPLAHPVPDLDADLEDRPIGGLGIHLVRELAQDARYHRDAGWNHMRVVLEAIPSPT
                     "
     gene            22773..23159
                     /locus_tag="PK399_03940"
     CDS             22773..23159
                     /locus_tag="PK399_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPW12306.1"
                     /translation="MAISVRIHSLLPNGSQRVEVGGRLDTHTYGELDDRLTLVLAAKV
                     QSLVLDLAQLDYVSSAGVRSIFRARKLLAARGGTLVLANTQPQVQKVFDIVKAVPLSE
                     IFRSVEEADAYLDKIQKRIARGDQDD"
     gene            complement(23167..>24618)
                     /locus_tag="PK399_03945"
     CDS             complement(23167..>24618)
                     /locus_tag="PK399_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPW12307.1"
                     /translation="YQEQVMQIAQVLAGYSLGGADLLRRAMGKKKPEEMAKERSKFEA
                     GAAERGVDPRTATQIFDLMEKFAEYGFNKSHSAAYALVAYQTAWLKVHYPAEFMAAVL
                     SSDMDNTDKVVGFLDEARTLGLKVLPPDVNASTYMFEATDSKTIRYGLGAVKGVGRGA
                     CDAISAARTNAGPFKDLLDFSKRVDAAGLNKRTLEALVHAGAMDALASNRASLMLQLP
                     EVLKATDQIARNRDAGIVDMFGNATGAPDIHIDLPTCSEWPLAQKLQGERETLGHYLS
                     GHPMDPHRDELQRLVGHDLSSLDALWEARPQEARRGWRQQMQAVVAGQVVGMRKRGDS
                     QGFVQLEDGRGRIECAFFGDEWKEYAPLLTRDRILVVEGDLREDEFSGGFALRASRCW
                     DFTQLCGERAQRVSIRIDLRQPDALQHFERMLQAHAGATPLLIEAITAAGIGRLTVNG
                     GRGLRVDAALPGLLRNLPGVDAVSVTLAKPWVN"
BASE COUNT         3594 a         8227 c         8725 g         3972 t
ORIGIN      
        1 ctgcccgacg tcgcatctcc gcccctcatc gagcccgcac ccttgaccac caccgcccac
       61 gtcatcaaga ccagcctgcc cacccctccg ccttcggtgg aaacgctgct caagaccgtg
      121 cacgccgcgc tggaggaact caaggccaag gacaccgtcg aaatcgacgt gcgcgggaag
      181 agcagcgtct gcgattacat ggtcgtggcg tccggcacct ccacccgcca cgtcaagtcg
      241 atcgccgacg aggtggtgcg attcgccaag aagctcgacg tcatgccgct gggcgtggaa
      301 ggcgagcgcg aagccgaatg ggtcctggtc gacctggggg acgtggtcgt ccacgtgatg
      361 ctgccgcggg tgcgcgagtt ctatgcgctg gagcgcctgt ggaccgtcgg cgaccagcat
      421 cccgactacg tcgcgtcgga cgcctgaggc gtactttgtc cttgatggct tccccgtgaa
      481 ggggagggac ggacgatgaa agcccgcctc gtcgccgtcg gtgaacgcgc ccccgcatgg
      541 gtggcgcagg ggttcgcgga ataccagaaa cgcctctcgc actggctgcc gttggaactg
      601 gtcgaagtcg agcccggcgt gcgcggcaag gggcgcgaca ccgcccgggc aatgagcgat
      661 gagggcgcgc gcgtgctcgc cgcactgccg aagaatgcgt tcgtggttgc gctcgacggt
      721 cgcggcaagc cgcattcgtc ggagcaactc gcggtaaggc tggagcactg gcgcgggcgc
      781 ggccaggatc tcgcgttcct ggtcggcggc cccgaaggac acgcacccga agtcatcgcg
      841 cgtgcggacg aaacctggtc gctgggcccg ctgaccctgc cgcacatgct ggtgcgattg
      901 gtgctagccg agcaggtgta tcgcgcggcg gccctgctgg cgaaccatcc ctaccatcgc
      961 gcctgaacgc gagacgcccg catcgacgat gcgggcgtcg tgttcttcga gcctgtgaga
     1021 tcagcgcagc gcgaattcga tcggcaccat cccgatggcc tgcacggcct ggccgtcctt
     1081 ccgcgccggc tggaaactcc agcgcttcat cacctgcgcg cgggcggctt catccagctc
     1141 gcgatgaccg ctgctctgcg ccacggtcac ttcgagcggg cgaccatcga ttccgaccag
     1201 cacctgcagc aacaccgtgc cggtgaggcc gcgctgcttg gccatgcgcg ggtagtccgg
     1261 tgccggcgcg ctcttgtatt cgagctggat cggcgcgggg ccagtggcga tcggggcgat
     1321 gtcaccgacc gggccggcat caacgaccgt gctttcgatg gcctgttcgg tcgcgccatc
     1381 agtcagcacg ggggtcgcaa cggctgtcgg ttgctgggta atggtttgct ggcgcactgg
     1441 ctgcgtggtg ggtggcgtcg gctgctgctg gaccaccggc accttgatcg gcggcggcgg
     1501 atcccgcctg atccagtccg gcaggcgtgt atcgggtgtt tcatcgggca acggcagcgg
     1561 cgctggcatg gtcatcggca tcatcagcag gcccatggcc aggacattga ccgcgatggt
     1621 gccgctcatc gtgatgatgc gggccgtgtc gagttgcggt ttggaggcgg gcttcagcag
     1681 cgcgaaacct gccgggcgga acgaagcgat ggagtgcgag cgagccatgg tcaacctcct
     1741 gtcaggtggg tatgccatgc aacgaaccag gggcggcgat ggggtcgatg cccgtccggt
     1801 gcacgcttcg acgctgcgcc gtacgccggc gcattggcaa tcggcgggcg atgaccggca
     1861 gtctgccgcc gacaaatggc aatgactctc gttcatgttt tttgccccgg atacaacgat
     1921 gcccgcacat ggcgggcatc gtgggcgagc ggtgggcgac tcagggcgtg ggatcgggat
     1981 cgtgttccag cccaccgtcc tctgcatccg gttcgggtct ggcgcccggg atctgtgcct
     2041 gcgccttgtc cttcggcttc ggttccgcgt aaccggtgtt gcatgccgga tccggggagg
     2101 cggtcggcac gctggcggcg tcgctgtcct tgcgggtggt catctcagcg ccccaactcg
     2161 aagaccacgt cgatgctcac cgacagcgac gcctcgcccg gcgacaccga ggtttccttg
     2221 gcgaaaccgt cggaggccat cgcccgcatc atcggcatcg gccgcgggaa gctcgcaccg
     2281 ccctcgctga tgctgacgat gcggcgcacc ttcatgccca gcgcggcggc gtaggtgtcg
     2341 gcgcgcgcct gcgccttctt cagcgcgccc aggcgcgcct cgtcgtaggc cgcgtcgggc
     2401 ttgtcgactt cgaagctcgg gccgttgatc tggttggcgc cattggcgac gaatgcatcc
     2461 agcaccttgc ccagcttgga caggtcgcgc accttcacgt tgacggtgtt gctggcctgg
     2521 taaccgacga tgctcggcgg ctggttctcg acgtacttgt actgcgggtt gaggttgatg
     2581 ccggccgtct ggatatcgcg ctcggcgata cccgctgcgc ggatcgccgc catcaccttg
     2641 tccatctgca ccgcgttggc gcgcatcgcc gcgttggcgt cggttgcctg ggtgaccacg
     2701 ccggtggaga tggtggccac atcgggaacg cgggtggcat ccgcggacgc cgacaccgac
     2761 aacaacgtgc cctgcggcga ggcgatggcg atatccgcgg gtgtctgcgc gttggcggtc
     2821 atgggcaggg cgatggccag ggagagggcg aggaacaggg gacgcaggat catggtggac
     2881 tccgaatcgt tgcgggggaa gcgtgcgcga ggcgtggtgg gtgccaggtg aacgcgcgtg
     2941 gcgggagtga acgcgtggtg agccggaacg gggttgcagg catggccccg acatggccgc
     3001 gggttagact cccgaccatg ctgcatcttg cctcgcgctc gccacgacgc gccgaattgc
     3061 ttgcccgcct tgggctggac ttcggcatca tcgacctcga tctcccggaa cgccagggcg
     3121 ccggcgaggc ggctgccgag tacgtacgcc gggtcgcccg cgagaaggcc ggtgccggcc
     3181 tgctgcaggt ggtcgcggtg ccgggtgcgg tggtattggg cgccgatacc gaagtgatcc
     3241 tggatggccg cgtgttcgga aagccggccg atgccggcga tgcggcagcg atgctgcgca
     3301 cgctctcggg gcgtacccac gaggtcatca ccgcggtgtc gctggtctcg gccgcgcgcg
     3361 aggcgcaggc agtgtcggcc tcgcaggtga cggtggcggc attggacgac gctgcgatcg
     3421 ctgcttacct cgccaccaac gaatggcagg gcaaggccgg tgcatatgcg atccagggcc
     3481 gtttcgaatc gcacatccag cacctctccg gcagtttcag cggtgtgatg gggttgccca
     3541 tgttcgagac cgcgcggctg ttgcgcgagt tcggcatcgc cgcatgagca cctgccacga
     3601 tgagtgaaga actgctggtc aacgtgaccc cgcgcgagac ccgcgtcgcg gtggtcgaga
     3661 acggcatgct gcaggagctg cacatcgagc gtggcagcca gcgcggcgtg gtcggcaaca
     3721 tctacaaggg caaggtgcag cgggtgatgc ccggcatgca ggcggccttc gtggacatcg
     3781 gactggaccg cgcggcgttc ctgcatgcca acgacatctt ccgcagcgcg cagctcgacg
     3841 ccggcgaggt cgacgacgcg ccgggcgcgc cgccgtcgat gcaggtccag gtgccgatca
     3901 ccgaactgct gcgcgatggc gcggagatcg tggtgcaggt ggtcaaggat ccgatcggca
     3961 gcaagggcgc gcggctgacc acccagatca gcatcccctc gcgttacatg gtgctgttgc
     4021 cgcgctcgcg cgtgctgggc atttccgcgc gcatcgagga cgacgaggag cgcgcgcggc
     4081 tgaaggcgtt gatgaccgaa ctggcgcgtc ctggtggcgc gtacggctac atcgtgcgca
     4141 ccaatgccga aggccagccg gccgaagcgt tggccgagga catcgcctac ctcaagcgcg
     4201 cgtgggacct ggtcgaagcg acctcggcga ctgccaaggt cggcagctgc gtgtatgaag
     4261 acctcagcct gccgctgcgc gcggtgcgcg acctgatccg tcgcgacgtg gacaaggtca
     4321 aggtcgactc gcgcgagacc tgcgagcgcc tgcgcgaatt cgccgcgcag tacatgccgg
     4381 ggctcgcgga aaagatcgag cactactcgg gcgcgcgtcc gatcttcgac atgtacgggg
     4441 tcgaggatga aatccagcac gcgctggaga aggaggtgcc gctgaagtcg ggcggttacc
     4501 tggtgatcga ccagaccgag gcgatgacca cggtcgacgt caacaccggc agcttcctcg
     4561 gccagcgcaa cctggaagag acggtgtacc gcaccaacct ggaggccgcg caatcggtgg
     4621 cgcgccaact gcgcctgcgc aacctgggcg gcatcatcat catcgacttc atcgacatgg
     4681 tcgatgccga gcatcgccgg caggtgctgc gcacgctgga gaaatcgctg gcccgcgacc
     4741 acgccaagac cacggtgtac gacttctcgc cgctcggcct ggtggagatg acccgcaagc
     4801 gcacggtgga gagcctggag cgccagctga gcgagccctg ccacgagtgc agcggccgcg
     4861 gcacggtcaa gaccgcggag acggtgacct atgaagtgtt ccgcgagatc gtccgagcgg
     4921 tgcgccagtt cgatgccccg cggctgctgg tgatcgcctc gccgaaggtg gtggcgcgca
     4981 tcaccgacga ggaatccacc gcggtggccg agctggagga attcctgggc aagtcgatcc
     5041 gcttccaggc ggacgcgcag tacatgcagg agcagttcga tgtggtgctg ctctgacgct
     5101 tttgaattcc ctcccctgcg aaggcagggg agggctaggg tggggtgctc gttgctaaga
     5161 gagcaacccc tccccaaccc tcccctgcct tcgcagggga gggagttgaa tcaatgacca
     5221 cgcccgtccg ccgacgctgg cgcctgctgc gccgcggtgc ctggtacgca ttggccgcgg
     5281 cgttggtgct gctggcgctg ggcaatggga tcggcagcca gttgctgccc ttggtcgagc
     5341 gccatccgga caaggtggcc acctggctgg gcacgcgcgt caagagcccg gtcgcgttcg
     5401 accatgtcga gaccgaatgg acgcgtcgcg gtccattgct gcggctggac aacctccgca
     5461 tcggcgatgc agcgaatccg ttgcgcatcg gcgatgccga aatcctcgtc gcccagtacg
     5521 cggggctgtt gcccgggcgt tcgttcaccg agctgcgggt gcgtggcctc caactgaccc
     5581 tgcaacgcga cgccaacggg ctctggagcg tgcgcggatt acccggacag cagcaggccg
     5641 gcggcgatcc cttcgcaacg ctggagcggc tcggcgagct gcagctgtcg catgcgcggc
     5701 tgcgggtatc ggcgccagag ctgggtttgg acctgcgggt gccgcgcgtc gacctgcgca
     5761 tgcaggtgga cggcgggcgc atccgcgtgg gcgcgcatgc ctggttgcgc gcgggcgcca
     5821 ccccgttgtc ggcggcgctg gactacgacc gcggcagcgg cgacggccgg gtgtacgcgg
     5881 gcacgcgtcg tgccgacctc ggcgagttcg ccggcagttt cgacctggcc ggggtggtgc
     5941 ctgtctccgg cggcgggcgc ctgcgggcgt ggggacagtt gcaggcgcac cggatcgtcg
     6001 gcatccgcgc cgatgccgcc ttgcaggacg tggtggtgcg cggcgccccg tcggcggatg
     6061 gacaaccgcc gccgacccaa gcgctgggca aggtcgccct ggacgcgcgc tggagtggca
     6121 ccgtgcgcca atggcaggcc aaggccacgc gcttgcgcct ggggtcgggc gatgccgagc
     6181 aggtgatgga tggtttggtt gtccggggcg ggcaacgcta cggcctgcag gcacggcgca
     6241 tcgacgccgc gccgctgctg cagttgctcg cgctgggcga tgtgttgccg ttgggcatgc
     6301 gcggctggct gcaggcgtca tccgccggcg cggtgctgga agacgtggat gtggagggcg
     6361 tgcggggcgg cgtggtctcg gcgaccgcgc gtatccgcgg tttgcatttc gccccggtcg
     6421 gcaacgcgcc gggcatgcgc ggcgtcgatg gctggttgca gggcgatgcc gacggcgtgc
     6481 gcctgcgctt cgatccccag gccgtgttcg cgttcgactg gcccgccggt ttcggcgtgg
     6541 tccaccagtt cacggctgat ggcgaggccg tggcgtggcg ggacggcaac ggctggagcg
     6601 tgcgcaccgc cggcttggcg ctggtcaatc cggacctgca ggtgaaagtg cgcggtggca
     6661 tggggttcgc gaatgatggc acgcgtccgc gcctggacct ggcggccgac ctggggaccg
     6721 cccaggtctc gctggcgcac ggcttctggg tgcaccacct gatgccgaag tcgaccgtgg
     6781 actggctgga tgcggccctg aagggcggca gcctgcatga catccacgcg gtggtcgccg
     6841 gcgacctgga cgattggccg ttccggaccg agggcggccg gcagggtgcc ggcgtgttcc
     6901 gggtcgatgc ccgcatgcag ggcggcgtgc tgaaattcca gccggactgg cctgcagccg
     6961 aagacctgga cgccgacatc cgcttcgagg ccgatggctt caccgtggcc gggcgcagcc
     7021 ggatcgccgg tgtgccggtg gcggcactca aggccggcat cgcccgcttc gggcgtgccg
     7081 agctgacggt cgacgcggcg gcggcgggcg acgccaagga cttcctggcc atgctgcgcg
     7141 ccagcccgtt gcagaagtcg tacggcgcga gcatggacag cctgcgcgtg gccgggcccg
     7201 cgcaggcgca gttccacatg ctgctgccgt tccatcacga caagccgcca ccgcacgacg
     7261 tccacggcga cgtggtgctg tccggcgcaa gcctgcgcga cgaacgctgg aagctggcgt
     7321 tcgagcaggt gcgcgggaac atccgctacg acaacgcggg cttcgctgcc gagggcatgc
     7381 aggtgcgcca cgaaggcacg cccggcgtgc tcgccttgcg ggcaggcccc cacgtgcgcg
     7441 accgcgcgca cgcgttcgag ggcgaactgg acgcgcagat cgacatcgat ggactgctgg
     7501 acaaggccgg cagcctggcc tggctgaagc cgcatgtgtc cggcagctct gcatggacgg
     7561 tgaccctcgg catcccgcgc aatgcggcgc agggcgcgat ggccggtcgc ctgcaattgc
     7621 gctccggcct ggtcggcacc gcactcgacc tgccggagcc cctgcgcaag ccgcgcggcc
     7681 agccgctggt ggccagcgtc gacctgcgct tgccactcga ggaaggcgag gtcgaggtcg
     7741 cgttgggcga cctgctgtcg ctgcgcagcc ggagccgcgg cagcgcgacc ggtgttgccg
     7801 tgcaattggg aggggcgcgt gccgacgccc cccccgcgga cggcctggtg gtcggcggtc
     7861 gtgccgcgcg cgtggatgcg ctggactgga tcggggtgat cgccggcgat cgtggcggcg
     7921 atggcttgcc gctgcgccgg atcgacgtca atgcggcgca actgcgcctg ctgggcaccc
     7981 agttcgccga cgcgcgcgtg caggtcgtcc cggcaccgcg cggcaccgcg gtggtggtgc
     8041 agggggcctc gctggcggga tcgctgctcg tgccatcgca ggaaggtgcc accgtcgccg
     8101 gtcgcttcga ccgcttgcac tgggcaatgc ctgcgcggag cggtggcctg ttggcgggcg
     8161 tgcagccgcc ggccgccaac gtgccggccg cgacgcctgc ggccgcaggg gcggaggtcc
     8221 ccgcattcga tccgggcaag ctcccgccgc tgctcttcga tgtcggtgac ttgcgcatcg
     8281 gtgaggccgt gctggggcag gcgcgctttc gcagcacgcc ggtggccggg ggcctgcgca
     8341 tggacgagtt caccacccgc ggcggcacgc agcggctgac cgcgaccggc acctggagcg
     8401 ggcggggcgt ggcggcgcgc acgcggctgg cgcttggcgt ggacagcgac gacatcggcg
     8461 cgctgctggc tggcctgtcc ttgggcggcc aggtcggcgg cggcaagggc aaattgcagg
     8521 tcgacgccga ctggcgcggt ggaccggacg ccctggatcc actcaccctg caggcgaacc
     8581 tgcggctgga tgcgcgtgat ggccgcctgc tggagctcga acccggcgcc ggccgcgtgc
     8641 tgggactgct cggcatcggc cagctgccgc gccggctgac cctggacttc cgcgatttct
     8701 tcgacaaggg cttcgccttc gacagcatca ccggcgacgt gcgcctggcc aacggcagcg
     8761 cacgcagcga caacatcgcg atcaagggcc cggccgcgga catcctggtg cgcggcagcg
     8821 cggacctgcg cagccagcgc ttcgaccaga ccgtggacgt attgccgaaa tcgggcggcc
     8881 tgctcaccgc ggtcggcgcg ctcgccggcg gtccggtcgg cgcagccgtc ggtgcggtcg
     8941 ccaatgcggt gctggaaaag cccatgcagg gaatcggcgc caagacgtat cgcgtcactg
     9001 gaccgtgggc cgcgcccaaa gtcgaggtca gcgcgcgtcg cgatccgccc aagacgcagg
     9061 tcgccgcgcc cgatgcgccc gggtgattcc ggtggcgcca tcgtcatccc gttgcaacgc
     9121 attccgaagc aggccccatg aacgcactcg ccatcgccga atcccgcttg ctggctcccg
     9181 ccggcctcga taccgacgcg ctgcaacgcg cgttcgccag cctgctgggc ccgggcatcg
     9241 acttcggcga cctgtatttc cagcatgccc ggcgcgagag ctggagcatg gaggacggca
     9301 tcgtcaagga cggtgcgcat gcgatcgagc agggcgtggg ggtgcgcgcg atctccggcg
     9361 agaagaccgg cttcgcgtat tcggatgacc tcgatgcggc ggcgttgatc gcgtccgcac
     9421 aatcggcgcg ggcgatcgcg cgcgatggtc gccagcatgc cgggcaggcg ctggcgcgga
     9481 gcaacggacg ccagctgtat cccgcgctgg atccggtcga tgcgttcgcc aacgaggcga
     9541 aggtcgcatt gctgcggcgc gtggatgcgc tggtgcgcac cctggatccg cgcgtgcagc
     9601 aggtgatggt gagcctgagc ggcggcgtgg acaccgtgct ggtcgcgcgc agcgacggcg
     9661 tgctggccgg cgatgtgcgc ccgctggtgc ggatgaacat ccaggtgatc gtggagcagg
     9721 ccggacgccg ggaatccggc ttcgctggct tcggcgggcg ttattcctac gcgcacttgc
     9781 tggccgacga caagccggag acgttcgccc gcgaggcctt gcgccaggcg ctggtgaacc
     9841 tcgacgcgat cgatgcgccg gccggcacga tgccggtggt gctcggccac ggttggccag
     9901 gcgtgctgct gcacgaggcg gtcggccatg gcctggaggg cgacttcaat cgcaagggca
     9961 cctcgaccta tgcgggccgc atcggccagc gggtggcggc gaagggcgtc accatcgtcg
    10021 acgacggcac gctcgacggc cgtcgcgggt ccctcaacat cgacgacgaa ggcacgccaa
    10081 cgtcatgcac gacgctgatc gaggacggca tcctggtcgg ctacatgcag gacacgctca
    10141 atgcgcggct gatgggcatg gcgccgaccg ggaatggtcg ccgcgagtcc ttcgcgcacc
    10201 tgaccatgcc gcgcatgacc aatacctaca tgcgtgccgg cacgcacgaa ccggaggaga
    10261 tgatccgctc ggtcaagcgc ggcctgtacg cggtgaattt cggcggtggc caggtcgaca
    10321 tcaccagcgg caagtacgtg ttcaccgcca ccgaggccta cctgatcgag gatggccagg
    10381 tcaccgcgcc ggtgaagggc gcgaccctca tcggcaacgg ccccgagacg atgcagcggg
    10441 tggcgatgat cggcaacgac atggcgttgg acgatggcgt cggcatctgc ggcaaggacg
    10501 ggcagtcagt gccggtgggc gtgggccagc cgtcgctgct gatctcggaa ttgacggtgg
    10561 gcgggacgca ggcgtgagct caggcgtcct cgtcctccag cgggtccgca acctcatcgc
    10621 cgtcggtcgc cagcagttcg cgcacttcgc ggaacagctc ccggtacgcg cgcggcggct
    10681 tgttgcgctt gcgttcctcg tgcgcgttgc gcaccaactg gcgcagcttc tgccggtcgg
    10741 catcgggatg cgcggccaaa aactcggtca tcgccacgtc gccgtcgtcg cccatcaggc
    10801 cgtcgcgcac cgcttccgcg cgatgcaggg cggcggtctc gcgtttgccg gcatcgccgt
    10861 ccttggccag cgcgtcgcgg atcgcgtcca gcaccgcgtc gtcctggcga cgcatctgct
    10921 tggccaggaa cgcgtgttgc cgcttgcgcg ccccatgcga cgtcatgcgc tgggtctcgc
    10981 ggatatgcgg cagcagttcc tcggggatcg gcagccgcgc cagctgcgcc gcgctcaacg
    11041 cggtcagggt ctcgcccagc gccagcacgt ccagcgcatc gcgccggttc tggctgcggc
    11101 tggggctcag gaattcgccg gtgtcttcat cgcgtccacg catcagtaca ggatagtcca
    11161 ttgaacagcc ctgccatcga aatcgccccg gtcgccgacg acagccgcga gcgcatgcaa
    11221 cggctctccg gtatcgccga gcagttgctg gcccaggccc gcgccgccgg tgccagccag
    11281 gccgaggtca gctgcaacga ggacagcggg cttgccgtca acgtgcgcat gggcgaggtg
    11341 gaaaccgtcg aggccacccg cgaccgcggc atcgccgtca ccgtctactt cggcacgcgc
    11401 aagggcagcg ccagcactgc cgacctgcgc gaggacagcc tgcaggcgac ggtgtcgcag
    11461 gcctgcgcga tcgcccgcca taccgaggac gatccggcct ccggcctggc cgacgccgga
    11521 ttgatggcga ccacgctgcg cgaattcgac ggctggcatc cggcgaattt cgatgctgac
    11581 cgtgcggtgg cgatcgccct ggccgcggag gccgcgggcc gcgaggtcga tgcccggatc
    11641 ggcaattccg acggtgcctc cgcgaatgcc ggcgcgacca tcagcgtcta cgccaattcg
    11701 catggcttcc tgggcgccga gcgcagcacc tcgtacagca tcggctgcgc gctgatcgcc
    11761 ggtgaaggcg aggacatgca gcgcgacagc tggtacagcg tggcactggc ggagggtgac
    11821 ctcgaaccac cacaggcagt cggccgcaag gcggcgcagc gcacgctgga gcggctcgat
    11881 ccgcgctcgc tgccgaccgg cgaggtgccg gtgctgttcg ccgcggagat ggcgcgctcg
    11941 ctggtcggcc actacctcgg cgcggtctcc ggcggggcct tgtatcgcaa ggccagtttc
    12001 ctgctggaca gcgtgggcac gcgggtgttc ccggactggt tcgacatcga ggagcggccg
    12061 ttccttccgc gcggtttccg ctcggccgcg ttcgatgcgg agggcgtggc gacgcgcgaa
    12121 tccccgctgg tgcgcgacgg tatcgtccag cgctacgtgc tgggcagcta ttcggcgcgc
    12181 aagctgggcc tgcagaccac cgccaacgcc ggcggcgtgc acaacctgca ggtgcgggcg
    12241 aatgccggcg gcctgcagga actggtgcag ggcatgggcc gcggcttgct ggtcaccgaa
    12301 ttgatgggac agggcgtgaa cccgatcacc ggcgactact cgcgcggcgc ggccgggttc
    12361 tgggtggagg gcgggcgcat cgcctatccg gtcgacgggg tcaccatcgc cggtaacctg
    12421 aagcaggtgt tcgccgcgat cgaggcggtg ggcaacgatg tggatccacg ttcgcacatc
    12481 gcgaccggtt cgatgctggt cggccgcatg acggtggccg gcaccggctg aagcggggtc
    12541 gggcgcgggg tcgccggcat cgcgccgatg gccggccggt ggcgccgcgc aacggcggcg
    12601 ggactggccg gcacctgcgt cgggccgcgt tatgcttcgt gaaccgcgcg ctcgcgcaaa
    12661 accggggaac tctagatgga tatcaacgaa gccaccgcac ctcctcccgt gccttccggt
    12721 gcgccgtctt ccgatgaccg gcagtggggg atgttcgcgc acctgtcggc gctctccggc
    12781 gttgtcattc ccttcggaaa catcatcggc ccgctggtgg tgtggcaggt caagaaagac
    12841 acccttccct tcgccgccga ccagggcaag gaagcgctga atttcaacat caccatgctg
    12901 atcgccgcgt tcatcggctt cctgttgacg ttcatcctga tcggcctggt gctgctgccg
    12961 ctgatcggca tcgcgtggct ggtgttcacc atcctcgcgg gcatcaaggc caacgaaggc
    13021 gtcgcgtatc ggtatccgta cgcgctgcgc ctgatcaagt aagcggtccc cgtcgccggc
    13081 gttgcgcgtt tcgtcgcgcg ccggcggcct ttcgtcgccg ctggcccgca ttgggcctcc
    13141 ggccgcttgg atcgccaggc aatgcctcca tcctcctttc catgccggac tgtttccggt
    13201 gggagtcgac gatggaaacc agcacctacg aatccggtgg cgccgcgacc gccgtgcaga
    13261 ccctgtcccc gagcctcacc gaccgccagt gggcggccgc tgcacacgct gcggcgctgg
    13321 tggccgcgat ggcgaccagc ctgtgggcgg gcgtggcggg cgccattgca gcagtggggg
    13381 tgtggatgct ggtacgcgac cgctccgcgt tcgcttccga gcatgcgaaa gaggcggtca
    13441 acttcaacct cagcatgttc atctatgccg cagccgcggt ggtgttctcg atcttcaccc
    13501 tcggcatcgg cctgatcgtg gcgctgccgt tgtgggggat cctggcgctg gtgtggatcg
    13561 tctgcaccct ggtcgcggcg ttcaaggcct acgatggcca gccgtaccgc tatccggtga
    13621 cgatccgcct gttcaagtaa gcatcgccca gcatcgatgc agggcgcatc gggtgaacag
    13681 gaaaggccgg cgcaggccgg cctttccgca tctgccgggc ctgccgcgat cagcgatcca
    13741 ggtagggctc caggcccttg taaccctgct tgtccagcgc atccaggcgc ttgtccagcg
    13801 gaggatgggt cttgtacaac tgggtcagtc gttgtgatga agacccgagc gcggtcatct
    13861 tctgcagcac cgcgtaataa accagcggat tcgcgccggc gcgcgccagg tagaagccgg
    13921 cggcctggtc ggcacggtat tcggcgttgc ggtcgagttg ggtcagcatg atcgctgcac
    13981 cgttccgttc gatgtagccg cgcgcaaggc tgccggccac cccgccgccg gtgttgaccc
    14041 ggctcagcgc gacatcgcgg cccgcgccct gcaactcctg cttgcgcagg acttcgtagt
    14101 ggtcgcgctg gaccacgtgg ctgagttcgt ggcccagcac cgcggccact tcgtcgtcgc
    14161 tgccgagcaa ctggtaaagc ccgcgggtga tcaggatgta gccacccggg gcggcgaagg
    14221 cgttgatctc gccatcgtcg atcacgccaa aggtccaggg caactcgggg cggctgctgc
    14281 gcgaggccag ccagcgaccg atcaggttca cccgcttctg catcgccgga tccttcacca
    14341 acggtgccgc gccgaggacg cgaccggcga tcagcgggcc cagctccagt tcttcctggg
    14401 cgacttcgtc ttccgacttg ccgatcagct tgtcgccgga ggccgctgcg gtcgcgccca
    14461 cgccaccgcc cagccggccg ccgaccgagg acagcagccc gcgcgccatg ccgaaccgct
    14521 cgcgcttctc ggcctgggtg gcgccaccct tgccgctgcc cttcgccttg ccaggctggt
    14581 attcgatggc gtacgccacc tgcgtggccg tccagccgcg cttggcagct gcctgttcgg
    14641 cggcttcggg ggtggcgcgg taggactcca tcagcttcag ctgggcggca ttgaagctgg
    14701 cggccttcag gtcgctctcg tcgatgccgc gcacgctcgc ggtttcgctg acgcggccct
    14761 tgctggcctt gccggtgaac agcgcctggg cagccccgcc ggtttcggcc ttgccgtagg
    14821 ccacgcgcac gtcgttgacc cgtacgtagc cggtcttgcc accgtccaac gccagctgga
    14881 accacaggcc ctgctggccg ctgaccttga ccggcgcgtt cttgctgaac gtggccacgg
    14941 tcggggaggc gaaatccggc ttggcatgcg cctcgatcga aggcttggcg actgtcgcgg
    15001 gggtgatcgg gtcggcggcc agcagcaggc cggacaccat cgccgccagc acggcggcac
    15061 tccatcgcat cgtgttgcgt gtcatcgatc ttccccctcc gcgtcggtgg catccgtcgc
    15121 ctcgtcgctg aactccagcg tgtgcgacgg atccagaata tagcgattgt ccgcgaccag
    15181 cagccattcg tcggtcgcat tcaggtcctg ccccagcagc tcgcgcaaac gcatgcgctc
    15241 gccgcggttg gtcaggtcgt tgtcgtcgtc gatctcggca acggaaatgc agaccatcac
    15301 ccggccatgg tcctccaggc gcaggtcgcc ttcgttgagg tcgcgataca actggtcgag
    15361 ctcgcgcttc gccgcgtcca gtgcctgggt gcgctcggta ccggtccaga tcagcagcat
    15421 caggtcgtcg aggatgtcgt gcagccagct cttgcgctcg acgatgccct cgatcatcac
    15481 cgcgtcgctg ccggcataca gttgccggcg cagcaggcgg cggtactcgc gcttgccgcg
    15541 gatcgcggcg aatgcctcga tgtcgccatt gggcacgaag cgcaggcgcg ccatcaacag
    15601 ccagggatgg tgctgcgggt tgtattcgtc gcgatagtcg ttgttgccga tcgcgcggcc
    15661 gcgctgcacg gaggcgtaga gccggcacag gccgaagcac aggctgacga agccgacgct
    15721 ggcgaagatg tcgacgaaca ccccgaagaa ggtcaggccg acgaaggcga tgagcaggag
    15781 ggtggcgttg gtggccacga acaccccgtc cacgtcctca tgcggttcgc cgcgccagaa
    15841 tgcgtagctg gtcagcagga ccagcagcgc cgccagcagg tacttgaacg aggtcggcgg
    15901 catccagatc gcgctgtcct gcagcagcgc ctcggtcgcc tcggcccaga cttccacgcc
    15961 cggcatggcg gccttgatcg gtgtcggttt ggtgtcgctg atgccggccg cgctgtagcc
    16021 caccagggcc acggcgtcgc gcaacacggg gcgctttgcg cctgcggcat ggcagaccgt
    16081 ctcgcccgcc agcaggtccg cggcactgat gtagggcagt tgcgtgcgct tgcgccagtt
    16141 cacccggacc tgcgcggggt aggaggaggg cgaccgccct tgcacctgcg cggcgagcag
    16201 caccggcagg gtggggatag cccagccttc gacctcatcg cgcaagggca cgtcgcggac
    16261 gatgctgtcg tccgagcgac cgaagttggt cgatgcggca tgcttcgcca tcgcatcgcc
    16321 gtacggcagc tgcagcggga tcggcggtcc cggtggcctg gcctcgggcg acaacggata
    16381 cgcccccggc gcatctgcgg cacggatcgc ggccttggcg ttgaaatccg cgtcctccgg
    16441 gaacgtggcg gcgaacagga accgcccctc gccaccggcg gcgaccaggt ccagcatgcc
    16501 gtcgccgttc gggtcatccg gatcgcgatc caggaacatc acatccacgc cgaccgcctt
    16561 caccccggcg cgatccagcg catccagcag gtccgcgtgc accgatcgcg gccaaggcca
    16621 gccgcccagg ccctgcgcgc gcgtccactc gatcgagcac tcgtcgatct ccacgactac
    16681 ggtcttgccc gacggtttcg gctccaccgg ccgcagtttc acgaccttgt cgaacaggct
    16741 gtcctccgcc gcgtccaggc tgcgcccgtg ggtccagtcc cagcccagcc agcccagcgc
    16801 cgcgatggcg agcagtgggt agaaggcgaa cttgaggcgc agcgcgagct tcgacaacgg
    16861 ccgccgccag ccacgttcga ccacggtcca cgcatccatg aaccacaccg ccagcgacgc
    16921 catggtggcg tcgatgcggt cctgcaggag cttgaaggcg cggcgtgcca tgccggtggc
    16981 tcagcggtcg cgttcgacga cgaggcgggc cagcgcggcg agcgcggtgg tgtcgacggc
    17041 gagcgacgac aacgcgtgct gcatcgtgtc cgccagcgat gccaggcgcg cgcggctggc
    17101 gtcgatgccg agcagcgcgg gaaaggtcgc cttgtcctgc gccgcatcct tgcccgcggt
    17161 cttgccgagg gtggcggcgt cgccttccac gtcgagcagg tcgtcgcgca cctggaacgc
    17221 caggccgagt gcgtcggcgt agcgatccag cgcgccgcgg ctggcgtcct cggctccggc
    17281 cgcgatcgcg ccgaggcgca ctgcggcgcg cagcagcgcg cccgtcttca tcgcgtgcaa
    17341 gcgttcgagg tcggcgagaa ggaaagtggg gtcggagtcg cctttcgcag acgaaaggcg
    17401 actctgaccc cagtttcccc gaccccactt tccggtcgca tcgatatcca gcgcctgccc
    17461 gccgcacatg ccggccacgc cggaggcgcg cgcgagttcc gccagcatcg cgacgcgtcg
    17521 ctccgccggc tgcggcgcgt tcgccaatac ttcgaaggcg cgcgcctgta gtgcatcgcc
    17581 agcgaggatc gcggtggctt cgtcgaaggc gacatgcacg gtgggctggc cgcgacggag
    17641 ggcgtcgtcg tccatcgccg gcaggtcgtc gtggaccagc gagtagcagt ggatcagctc
    17701 gaccgcgacg gcggcggcat ccaggtcggc ctcggtcgcg ccgaatgcgc ggcccgtggc
    17761 gtaaacgagc atcggccgca tgcgcttgcc gccgttgagc acgccatggc gcatcgcggc
    17821 gtgcaggcgt tcgggcgagg tggcggggtc gggtagggcg cggtcgagcg cggcctcgac
    17881 gcgcgtgcgc caggccgcga acatcgcgtc aggcatcttc gctggcgtcg aacggacgcg
    17941 cgtcatcggg attggcgggg tcgctgagca ggcgcacgcg cagttccgcc tgttccagcg
    18001 cggattggca atgccggtac aggccgacgc cgcgctcgta ggcggccagc gattcctcca
    18061 gcgtcatctc gccgctttcc atgcgcgcga ccaggtcttc cagcgactgc atcgattgct
    18121 cgaagtcggc aaccggcgag gtgtcttgcg gaggtgtcgt ctttcgggcc atgcgcgaag
    18181 tttacgccag cctcgcccag tgcagtcggg cgccgcgcgt gcccagccat gcggccagcg
    18241 acgccgacag gatggcgtcg ccggccgcga gcagggtgcc atcgttcgat gacagcagcg
    18301 gcaggcgttc gcgccgccag ggcggaatgc ctgcgtcctg caggacgtgc ttcagcgcat
    18361 gcgaatggcc acgtcccggc aacacgatgc gttcgccgcc ttgccgcgca tgcacgcgca
    18421 acggtgggtc gaaacgctcg gcgccgagca gggacagcgt gtcgccggtc ggcaggagca
    18481 gcggcgtcgt gccgtcccat gcctgcgacc attgcgcggg cagcggatcg cgcatcgcct
    18541 cggcatgcag caggtggcgc cagcggcgga tgcgtgcccc ggaccacgcg aaacaggcct
    18601 gggtatcggc gcgcgcgggc agcaggtcgg cttcgatgac ggcgatgccg ttgccgggca
    18661 ggggctgcag gccgagccca ccgatccagc gtcgcaggac gcgcgcgcgg cgttccggga
    18721 cgatcgcgcg cagtgcgtcg acatcgagcg tgtggtcgtc gcgcatcacg ccctgcaacg
    18781 ccgacacgtc atcggcttcc agcaggtccg cggcctgcgc attcaatgcc gcgcagcgcg
    18841 ccaggttgtg gctcgcttgc ggccagcgct ggcgcagcag gggcatcacg gcgttgcgca
    18901 ggaaattgcg atcgaagcgt ggatcgccgt tgcccgcatc ctcgatccac gccaggccat
    18961 gcgcggtcgc atgctcgtgc aattcctcgc gcgcgacatc gagcaagggt cgccacagcc
    19021 agccgtttgc gtatgcgcgc cacgaccgca tcgctgccag gccgtccacg ccggagccac
    19081 gcaaggcgcg cagcaggaag gtctcggcct ggtcgtcgcg atggtgggcg agggcgagga
    19141 tctcgtcatc ggccaatgcc tgcgcgaagg cgcgatgccg cgcgtcgcgt gccgcacctt
    19201 caagaccctg gccggatgca cgatccacat cgacgtggac gatcgacaat gggacgtcga
    19261 gcgcatcgca gctgcgctgg cactgcgcgg cccaggcatc ggcatcggca tgcagcccgt
    19321 gatgcacgtg gatcgcgccc aagccgttgc ggcgaatgtc ggtattcgct gcgagcaaat
    19381 gcaaaaggac agtcgaatcg aggccgccgc tgaagccgag catcacgccg cgcccatcgt
    19441 cgcgccggac cacatcggcc aacggcgcgt gcatgtgcct caggacgcgg tgtcgtaggc
    19501 gccgtagccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcatcagcgt
    19621 gcggtcgccg acgccgatcg ccagcgcgcc gccgctgccg ccttcgccga tcacggtgca
    19681 gatgatcggc accttgagtt cggccatttc cagcaggttg cgggcgatgg cttccgattg
    19741 gccgcgttcc tcggcatcga tgcccggcca ggcgccggcg gtgtcgatga aggtcaggat
    19801 cggcaggccg aagcgttcgg ccatcttcat cagccgcagc gccttgcggt aaccctcggg
    19861 cttgggcatg ccgaagttgc ggcggacctt ggccttggtg tcgcggccct tctcgtggcc
    19921 gatcaccatc accgggcggc cgtcgatgcg ggcgaggcca ccaacgatcg cgttgtcgtc
    19981 ggcgaaggcg cggtcgccgg cgagttcctc gaattcgtcg cagatcaccc gcaggtaatc
    20041 cagcgtgtac ggccgcgcgg ggtggcgggc gagctgcgag acctgccacg gggtgaggtc
    20101 gcggaagatc gtcgcggtgc gcttgcgcag cttttccttc aacgtatgca cttcggcgtc
    20161 gacattcacc gccgggccgt tgctggcatg ccggagctcc tggatcttcg cttccaggtc
    20221 ggcgatgggc tgttcgaaat cgaggtagtt cgggttcatc gcccgaagtt tagcccagcg
    20281 ggccggccat accgagccgt aggtggctgc gcgtggcgca atcgcttgac gcgggcaggg
    20341 gggcattgga cgctgcagcg atggatccga tgcccatgcc ggccgtgcca cccgcctcct
    20401 cggcggacgt gcgcgtgccc tggcatgcca gcctgcgcac gcggctgatg gcctgggtgg
    20461 gcctgttgct ggccttgctg ttgctggctg gattcgccac ggccttcctc gccgcccgcg
    20521 agcgcgtgct tgccgatgcc gaggcgcgca cccgctacga ggcgcggcag gcggcggatc
    20581 ggctggacgc cacgatgcgc tcggtccgcg tgtccggcga ggcgatgatt gaactcaaca
    20641 accgggtggc cctgacccgc gccgagttgc tggaggcgat ggaagcgatg ctggatgcca
    20701 atccagtcac ggtcggtggc ctggtggcgt tggaaccggg ggtgctggcc gaccgtgcgc
    20761 cgctggcgtt ctacgttggc atcgccgcgc gcggcgtgcc tgatcgcgac ctgctcgccg
    20821 acggttacga cctggccggg cgcgagtggt accagcgcac gttgcaggcg acgtcgccgt
    20881 ggtggtcgga gccgtatttc aacgaaaccg ccggcgggcg ctacatgacc acgctcaacc
    20941 tgccgctgcg cgaccgcagt gccaggcgca tcggcatggt cagcctggac gtgccggtgc
    21001 aggcgctgtc gtcgtcgctg gaatcgttgc gcacggtcac cggccagcgg accgcgttgt
    21061 tcgcaccgga gggcacgatc gccgtgcatc cggaacgcgg catcgccttc gcccatgact
    21121 tgccgggcta catcgcgcgt gccggtcgca gcgacctcgc cccgatggaa gccgcgcgcg
    21181 tgcaacagcg cccgctgcag ttcacccatg ccgacgcgcg cgacggcacg gtccgctatt
    21241 cggtgctgca gccggtcggc ggcagcggct ggaccctgca gctttcgatg ggccgcgacg
    21301 cgatgctggc cgggctggac agcgcgatgc gcatgctcgg cctgatcggt ggcctggtcg
    21361 cattgctggc ggcgctggcg gtattgcgac tggcccggcg gatcaccgtg ccgctgaccg
    21421 agctcacgac cagtgcgggc cacttcgcca atggcgaatt cgactggccg gtgccgcacg
    21481 atgcgcgcgg cgacgaggtc ggggtgatgg cgcgcgcgct ggaacgcgca cgcgattcga
    21541 tccgccagca actggacgag atcggacgtt acgccaccga acggcagaag ctgcagagcg
    21601 aactcgacat cgcccgcggc atccagatgt cgatgctgcc gcgcgaccgc gacttcgccg
    21661 ttggcgacat ccgttatcgc ctgcaggcac ggctggagcc ggcgaaggcg gtcggtggcg
    21721 atttccacgg tcatttcctg cagggcgccg accgcgtgtg gttcgtggtc ggcgacgtct
    21781 ccgacaaggg cgtgccggcg gcgctgttca tggcccgggt ggtcacggtg ctggaagtgg
    21841 ccaccgcggc gagcgatgcg cccgaccggg tgctggccga cgccgcacgc catctctcgg
    21901 agaacaacga cgcctgcatg ttcgccaccg tgctgtgcgg cgtgctcgac ctggcgagtg
    21961 gcgaactcgc gatcgccagc gccggccatg atgcgccgct gctgcggcat gccgatggcc
    22021 gcatcgacag tattccgctg gagtcaggcc ccgcgctcgg cttcgacgcg gtcgatgggt
    22081 tcgaggtctg gcgcggtcgc ctgcaaccgg gcgacctgct gttcgcatgg accgatggcg
    22141 tgaccgaagc cttcgatggc gacgacctgg ccttcggcga ggaacgcatg caggccgcgc
    22201 tgcgcaccgc gggcagcgcc gccgaagcct gcgacggcat gttgatcgcg gtgcatgcgt
    22261 tcgcggccgg cgtggcgcaa tcggacgaca tcaccgtgtt cgcgatcgat tgcgggtcgc
    22321 aacccgtgcc ggcggcgtgg tcgatggtgc tcgacgtgcc cggcggtcgc gatcggctgc
    22381 cggaggtgct ggccggggtc gaggcgcacc tgcatgccgc cggtatcgac agcggccgcg
    22441 tccacgatgc gcaagtgatc gtcgaggaac tcgcctgcaa tgtcatggac cacggggcgc
    22501 aggcgggcgt cgatgcgatg cgactcgacg tgaccgccgc ggacggtcgc gtcgtcctcg
    22561 aactccgcga caacggcgcc gcctacgatc cactcgcgca tccggtgccc gacctcgatg
    22621 ccgacctcga ggatcggccg atcggcgggc tgggcatcca cctggtgcgc gaactcgcgc
    22681 aggatgcgcg ctaccatcgg gacgccggct ggaaccacat gcgtgtggtg ctcgaggcga
    22741 tcccttcccc cacctgacgc ccacgcgact ccatggccat ttccgtccgc atccattccc
    22801 tgctcccgaa cggcagccag cgcgtcgaag tcggcggtcg cctggacacg catacctacg
    22861 gcgagctcga cgatcgcctg accctggtgc tggcggcgaa ggtgcaatcg ctggtgctcg
    22921 acctggcgca actggactac gtgagctcgg cgggcgtgcg ttcgatcttc cgtgcacgca
    22981 agttgttggc cgcgcgcggt ggaacgctgg tcctggcgaa tacgcagccg caggtgcaga
    23041 aggtgttcga catcgtcaag gcggtgccac tgagcgagat cttccgcagc gtggaagaag
    23101 cggacgccta cctcgacaag atccagaagc ggatcgcccg cggcgaccag gacgactgag
    23161 cgcggctcaa ttgacccacg gcttggccag ggtgacgctg accgcgtcca ccccgggcag
    23221 gttgcgcagc aggccgggca atgccgcatc cacgcgcagg ccgcggccgc cattgacggt
    23281 gagtcggccg atcccggcgg cggtgatcgc ctcgatcagg agcggcgtgg cgccggcatg
    23341 cgcctgcagc atgcgctcga agtgttgcag cgcgtccggc tggcgcaggt cgatgcggat
    23401 cgacacgcgc tgggcgcgtt cgccgcacag ttgggtgaag tcccagcagc ggctggcacg
    23461 cagggcgaag ccgccgctga attcgtcctc gcgcaggtcg ccttcgacca ccaggatgcg
    23521 gtcgcgggtg agcagcggcg cgtattcctt ccactcgtcg ccgaagaacg cgcattcgat
    23581 ccggccgcgc ccgtcctcca gttgcacgaa gccctgcgaa tccccacgct tgcgcatgcc
    23641 gacgacttgc cccgccacca cggcctgcat ttgctgccgc cagccccggc gcgcctcctg
    23701 cgggcgcgct tcccatagcg cgtccagcga cgacaggtcg tggccgacca ggcgttgcag
    23761 ttcgtcgcga tgcggatcca tcggatgccc gctgagatag tggcccagcg tctcgcgttc
    23821 accctgcagc ttctgcgcca gaggccactc gctgcaggtg ggcaggtcga tatggatgtc
    23881 gggcgcaccg gttgcattgc cgaacatgtc gacgatgccg gcgtcgcgat tcctggcgat
    23941 ctggtcggtc gccttcagca cttccggcaa ctgcaacatc agtgacgcgc gattgctcgc
    24001 cagcgcatcc atcgcgccgg catgcaccag cgcttccagg gtgcgcttgt tgaggccggc
    24061 ggcatccacg cgcttgctga aatccagcaa gtccttgaaa gggcctgcgt tcgtcctcgc
    24121 ggcactgatg gcgtcgcagg cgccgcggcc gacgcccttt accgcgccca ggccgtagcg
    24181 gatcgtcttc gagtcggtgg cctcgaacat gtaggtcgag gcattcacgt ccggcggcag
    24241 gaccttgagg ccgagcgtgc gtgcttcgtc gaggaaaccg acgaccttgt cggtgttgtc
    24301 catgtcgctg gacagcaccg cggccatgaa ctccgccggg taatgcacct tcagccaggc
    24361 ggtctggtag gcgaccagcg cataggcggc ggagtgcgac ttgttgaagc cgtactcggc
    24421 gaacttctcc atcaggtcga agatctgcgt cgcggtgcgt ggatccaccc cgcgttcggc
    24481 ggcgccggcc tcgaacttgc tgcgctcctt cgccatttcc tcgggcttct tcttgcccat
    24541 cgcgcgacgc agcaggtcgg cgccgcccag cgaatagccg gccagcacct gggcgatctg
    24601 catcacctgt tcctggta
//
LOCUS       DAOOCX010000025        24341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1743_length_24341_cov_8.646257, whole genome shotgun sequence.
ACCESSION   DAOOCX010000025 DAOOCX010000000
VERSION     DAOOCX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 24341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24341
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1743_length_24341_cov_8.646257"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1561)
                     /locus_tag="PK399_03950"
                     /pseudo
     CDS             complement(293..1561)
                     /locus_tag="PK399_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015472467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
     gene            1644..2240
                     /locus_tag="PK399_03955"
     CDS             1644..2240
                     /locus_tag="PK399_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPW12308.1"
                     /translation="MLGLLIFIAILAAVAFWGVGIYNGLVRARNAFRNAFAQIDVQLQ
                     RRFDLVPNLVEVAKTYMSHERETLEAVIAARSAAQSGLAAAKANPGDAEAMAQLAAAQ
                     GQLNAGLGRLMMVAEAYPDLKANQTMMQLTEELTSTENKVAFARQAFNDAVMAYNNKR
                     EVFPSSIFAGMFNFAAANLLEIPADRQSDVRAAPQVKF"
     gene            2296..4197
                     /locus_tag="PK399_03960"
     CDS             2296..4197
                     /locus_tag="PK399_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPW12309.1"
                     /translation="MNFFEQQAVARRTSARLVFLMAVAIAGIVLAVDAVVWVATQSPE
                     LVLFATLSSVAVIALGSLYRIASLGGGGEPVAQQLGGTLVPENTTEPGLRRLRNVVEE
                     IAIASGVPVPKVYVLEHEAGINAFAAGYSTSDAVIAVTRGALDRLNRDELQGVVAHEF
                     SHILNGDMRLNIRLVGVLFGILMIGLIGRKILEHGRFGGRGKNIGAILVAALVAMLIG
                     YVGLFFGRMIKAGVSRTREKLADASAVQFTRQTQGLSGALKKIGGLHDGSRLNQRSDA
                     EEVSHMLFGDGVGFDQLFAGKLFATHPPLLERIRALEPGFNDDAMARLQAQWRASPPD
                     GMQEDVALGLGGGSGARLPGATQALSVTPPMVAAQVAAPGADDYRRADSIVIAIPDAL
                     RDLARQRESVMPLLLGLLLAGDEALAERQRVDIAARLGGEVADHAVRIHQQLTADLHP
                     MLHLPLAGLAFPVLRLRPRPQLDAFMDTVQALVHADGQVSLFEYCLGRLLTVQVRESL
                     DPSRYARFGRRKPGNVRKEFATLLAVVAQAGHEDRASAQHAYLAGLQRVLPRDHLPYV
                     PPANGVLALDAVWEPLDALDPLAKQAMVEAVTAAVSHDGRVSVAESELLRTICAVLHC
                     PLPPMLERG"
     gene            complement(4277..5854)
                     /locus_tag="PK399_03965"
     CDS             complement(4277..5854)
                     /locus_tag="PK399_03965"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HPW12310.1"
                     /translation="MFDYIIIGAGSAGCVLANRLSEDPDTKVLLIEAGPRDWHPFIHM
                     PAGLAKLVNRKGVNWDYDTAPEPHLDNRTLWWPRGKVLGGSSSINAMCYIRGVAGDYD
                     DWGKAAAGWDWQSVLPYFKRAEGNTRGADALHGGDGPLAVSDLRHHNPLSDVFIEAAT
                     QVGMTRNGDFNGPTQAGVGLYQVTQRDGARCSSAAAYLAPVRSRPNLTVHTGALVSRI
                     TFEGRRASGVTYAMGGKAFHQPASREVILSGGAINSPQTLMLSGIGPADELRRLGIDV
                     VHDAANVGGNLQDHLDICTLQHSTQPITYDRINEPKMAFDYFLRGHRGAGSSNLAEAG
                     GFWRSPLATDDRCDVQFHFVPAMLDDHGRHRLAGDGYTLHACFLRPRSRGRLTLASNR
                     ASDKPRIHANYLGDAEGFDLKVMLECAKVSREIFAQPAFDPYRGTPIHPTRTDLSDAE
                     LVAFIRAKAESVYHPVGTCRMGDDDASVVDAALKVRGVEGLRVIDASVMPALPGGNTN
                     APTIMIAERAADLIKAG"
     gene            5977..7344
                     /locus_tag="PK399_03970"
     CDS             5977..7344
                     /locus_tag="PK399_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW12311.1"
                     /translation="MTETGTISTDALPWHRRAIAALAEILTLPSLRWSFLFFFCLLAG
                     YYVLRPVRDAMGASSEVEAVFPSAMIVFFAAHGIPLKDLVLQVLATCTFALILLAQPL
                     YGALVSRFPRRVFLPALFGFFIACLFVFYGLFHSNVSGRGMAFFLWVTVFNVFSVSIF
                     WSFMADVFASDEARKYYGYIGAAGTVGAFTGPILTRVLAEKVGLANLMLVSAGFLSVC
                     VVCIWRLRRWAVARERKAGRDNESAMGGDILAGLKLIFREPLLRWLATMVFLGVGVGQ
                     LLYNQQAEIAREGFASAEARTAYYAGIDIAVNVLTLVVQLFVTRWLLSRWGLAPVILI
                     PMAALLLGFAVLAASPLPMVVATVLVVTRSNEFSLMKPGRETIYTRVDRQWRYKAGAA
                     IDTAFYRGGEISFSWIYKALSMFGSHVVFGVGLAAAGVMTASAWRLLREEKKLPDEGP
                     RTEPG"
     gene            7344..7691
                     /locus_tag="PK399_03975"
     CDS             7344..7691
                     /locus_tag="PK399_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HPW12312.1"
                     /translation="MSMIAQLLIGLVALLHLYFLVLEMFLWTRPLGLKTFRNTPEKAE
                     TTRVLAANQGLYNGFLAAGLFYAIATGSREFALFFLACVIVAALYGAYSVNKRIFVVQ
                     GVPALAALVAVLL"
     gene            complement(7801..8268)
                     /locus_tag="PK399_03980"
     CDS             complement(7801..8268)
                     /locus_tag="PK399_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HPW12313.1"
                     /translation="MNVLASYRRISQWPAGRWLFSRLVCWKAPYFSSIAPRIDVLEPG
                     RGVATLRHRRAVTNHLGTVHAIALCNLAEFVGGLACDVSIPASMRWIPKGMTVAYLKK
                     AVGTMRATATPAFPPREASEGYDLPFDVVVENPQGEAVFTATIAMWVSPKAMR"
     gene            complement(8265..10739)
                     /locus_tag="PK399_03985"
     CDS             complement(8265..10739)
                     /locus_tag="PK399_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPW12314.1"
                     /translation="MSIALPFLAFFASGMFAAYHRLRLAVWAAMTATLLVGCWLLGAN
                     HVATIVAGVLVVLIALPLLVPQLRKPFITAPLLGFYTKLLPPLSDTERTALESGTVGF
                     EGQLFSGKPDWDQLLTLPKPQLTAEEQAFLDGPCEELCRMTDDWDICHVRADLPPELW
                     DYIKRNGFFGLNIPKEYGGLGFSALMNHKVIQKLASISSVVSSTVGVPNSLGPAELLM
                     HYGTEEQKQQYLPRLADGRDVPCFALTGPFAGSDATSIPDYGIVCMGEWNGINVLGIK
                     LTLNKRYITLAPVATLIGVAFRMYDPDGLLGDKKDIGISLALVPRDTPGLDIGRRHFP
                     LNSPFQNGPIHGKDVFVPLSQLIGGEDYAGQGWRMLMECLSIGRSITLPSTASGGSKM
                     GAVTVGSYARIRKQFGLSVGRFEGVEEALARIAGNAYATSALSEATAAAVARGENPAV
                     PSTIAKYHCTEMGRKAAMDSMDILGGKGIILGPRNFAGRSWQAAPIAITVEGANIMTR
                     SLMIFGQGAILCHPWVMKEMKSAQLADPKQRIDQFDAALFGHIGFAISNAVRSWWYGL
                     TGSQIGSAPGDDDTRRYFRKLNRYSAALAVMADTSMLLLGGKLKFKESLSGRLGDVLS
                     QLYIASAMLKRHQDIGNPAGDQPLLAYAIHDSVNKIETALSGALRNFPIRPVGWLLWL
                     VIFPWGRRAQAPSDRLGHKVASLLMTPCDARERLAEGVFTTPTANNPGGRIVSYLPKV
                     VMAEPVERKFLKALKNSDIEALEFDAQLDEGVREGWITADERKQLEELRALTLDAIMV
                     DDFDPEELRSAGYATLHGRIATRAAA"
     gene            10911..11489
                     /locus_tag="PK399_03990"
     CDS             10911..11489
                     /locus_tag="PK399_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW12315.1"
                     /translation="MTEAASRTGRLSADDWAREALDQIAEQGVASVAVEPLARRLGVT
                     KGSFYWHFPSRDALLQAALERWETAEQEIVFGSVENVSDPRERLRSLFQLVAHEVKSH
                     IIYSELLKALDHPTVSPVIGRVSQRRLDYLTASFRQAGLGRTAAQHRARLAYAAYVGF
                     LQLSLQLQQPRLHHDEFEAYVEHVMDTLIPQT"
     gene            11571..13322
                     /locus_tag="PK399_03995"
     CDS             11571..13322
                     /locus_tag="PK399_03995"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPW12316.1"
                     /translation="MSSRLDALNQWVLDVARLTRPEAIQWCDGSDSEYDALVAKMQDD
                     GTLLPLNPDTHPNSWLHRSHPDDVARVEHLTFVCTPEQEDAGPNNHWMSPADGHARMD
                     ALFDGCMQGRTMYVIPYCMGPIDSPLSRCGVEITDSPYVVANMRIMTRMGAAALARIE
                     REGSFVRGLHSIGELDPDRRFIMHFPEELAIKSYGSGYGGNALLGKKCHALRIASHQA
                     RQEGWLAEHMLILGLENPQGQTHYIAAAFPSACGKTNLAMLIPPEAYREAGWKVWTVG
                     DDICWMRPGADGRLYAINPEAGFFGVAPGTSAKSNPNALASIQSNTIFTNVGTTADNQ
                     PWWEGIDNDQHPATDWRGDPYDPSVRPAAHPNARFTVSARQCPSYSSMAEDPQGVPIS
                     AIVFGGRRASLVPLVFEARDWTHGVLVGASMGSETTAAAAGQVGVMRRDPMAMKPFCG
                     YNFADYFAHWLSFDKPGAKLPKVFHVNWFRKGPDGRFLWPGFGDNLRVLEWMIGRVEG
                     TAGAIETPIGLQPAEGDINLGGVDLGEDARSLLFGFDRAGWQSEFEGIRTYLHDYGPR
                     MPPPLLQEQARIENALG"
     gene            13522..16569
                     /locus_tag="PK399_04000"
     CDS             13522..16569
                     /locus_tag="PK399_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW12317.1"
                     /translation="MASNYFAKGLKQSALTIALGMCFVGGVQAQTNTGGNVVGSAAAG
                     DTITITSPSTGFSRTITAGADGNFRFAQLPSGQYQISQNGGAPRTVNVNVGTSANVDF
                     AGAATTLDTVTVTATSGAVNPIDVSSVESTTILTAEQVAKIPVPRNITSVALLAPGTV
                     RGAANAGFGALPSFGGSSVAENQFYVNGFNITNTFKNLAFAQVPFEGIAESQVKTGGY
                     GAEFGRSTGGVINMITKRGTNEFKAGGSIYYIPESLSETSPDLYYNNGLWVSNNSNDN
                     GWEATAAIWAGGALIKDKLFAYGLIQYGKTASETYTSRFGNTNLDSSSKRPQWLAKVD
                     WQISDDHSVELTAFSDKREFTDDYYRASFPDSTTNPTTGAITVGANPTTVFGDYRGTD
                     FNEAGGSSWVLKYTGYLTDNFTLSALYGHGEFSRSNYGVAANGQVQEYTGNLTGPVAG
                     CPIISDSRAGVSTGAVPAIRGCSFINTLGRRDALDERDQWRIDAEWQLGDHLLRFGID
                     NDKFRSVDGSAYSGGANYVYQSCNATAATCGPNGFRTRYRSFRSGANVGVDQQAYYIE
                     DSWSMTDNFVAYLGLRWDTFENLNGEGEKYVTIKNQFAPRLGFSWDVFGDSTFKVFGN
                     AGRYALPLTAGVATRAASASLYEERYYSYTGVDPATGTPTGLTPIVNPSTGSSAVRYL
                     NGEDGRPKFAAGYAVQNLKPMYQDEFILGMQKQLTDNFSLGVRAIHRDLGAAIDDNCD
                     YATLFEYVEGNGPGTKFNYCFLFNPGEDAEILADWNGDGTATVKTIPGADLSPKAKRK
                     YTALEFFVQGNWDKFFLQGSYTWAKSIGNTEGGVRSDNGQADTGTTSDFDYAAAMIGA
                     YGYTPNDRRHSLKLFGNWDITDQWSLGANLLVQSGRPISCIGVYQGLDVSHYGNIHFS
                     CDPGAPDGTGNNGSTIIGRGTVGRTPWTTNLDLNVAYKPSWAEGLQFKVDVFNVLNST
                     KVISVNEIGEDASGNPGPVANTYLTPTAWQSPRQVRFLVQYDF"
     gene            16672..18279
                     /locus_tag="PK399_04005"
     CDS             16672..18279
                     /locus_tag="PK399_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPW12318.1"
                     /translation="MTPEHLEALWRTGQQQFAQGLLAEARATYTRILAGSPRQVMVQI
                     ALSELEQRAGCYRAARLHALQAADTVAQTKRWEGLSHVTANLLSFDEREHVLHLIHQA
                     DWNDVRVLRQSPVVSQQLWLCGDQEAALRFLDIAQKRVKSDHRLAYSRAMALQHLGRI
                     EQASSAFEDSIRIAPDFALAHWSLAYHARSRPAGARLARLRAAARHVTSPQERAMLHY
                     ALYKELDAAGDCDAAWAELEAGASLMRDMLGYAPRKFESDVDAMLDAEVDAMLDAEAD
                     APLLPGPAVEGARTPVFVVGMPRTGTTLLTRILGAHPAVADADELNALEHAFAENVDR
                     FVDLPVARDLLPLLRAADQLGIAQAYHRRTQARYTAATTHLIDKNPMNLFAAGMIARA
                     MPNAKILCLVRGAMDACYSNLRQLFQNGAFPYSYDQVQLAERYAVFRRVVDRFAQRLP
                     GNFLAVSYEDLVRDPVETGRLAMEFCGLGFDPAYADTTRNSSPSGTASASQIREPIHT
                     HGIGEWRRYETRLRPLATRLAELGIAS"
     gene            complement(18428..18976)
                     /locus_tag="PK399_04010"
     CDS             complement(18428..18976)
                     /locus_tag="PK399_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12319.1"
                     /translation="MVPKVVELLVQGKCCESGVQRPQARRRQQSAQWHDMVFLWRLLA
                     QSRQRPPRLRQPGHLPQASLEIRGRALDVAMLRQHLATCQSCRDVGRLPGDRRLRACE
                     GEPMVIEVAQLQGACREQSRITVGRTNQRPRDRLHARRVIERAKPGDQVGKILVRALR
                     CIRRRKDRLMRRTIAPTERFDV"
     gene            19028..19885
                     /locus_tag="PK399_04015"
     CDS             19028..19885
                     /locus_tag="PK399_04015"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPW12320.1"
                     /translation="MSYAAGDSRALVDALSAMPTTTTTTTTVAHLQANARTPVFIVGM
                     HRSGTTLLEQLLDGSPQVRGVGELYDFTSAMRHATDHHCKGVIDATIVQRAAEVDFAQ
                     VGQRYLDGMAWRLGDQPFFTDKLPSNFLNIGFICRALPQARILHMVRDPVETCFSNLR
                     ELFSEANPYSYDQAELADYFLQYRRLMAYWHSAWPGRILDVDYAQLTRDPEKVMRQVA
                     AFCGIEYMDAMRDPRSSTRAVATASVVQVRERVVRREVPKWAPYARHLQPLIAALRQG
                     GVEVAEAFA"
     gene            19933..20508
                     /locus_tag="PK399_04020"
     CDS             19933..20508
                     /locus_tag="PK399_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW12321.1"
                     /translation="MPLPQDLQDPAQAAAEEDAALARRAAVADVRAFEQLYRRHHRRI
                     HGVIARLVGHAGERAEDLTQEAFVRAWQALPAFRFESAVSTWLHRLAVNTALMELRSR
                     RSRPWPEDDDEALESVATPDTAGRAMLGRDLERAVATLPPRARAVLVLHDVEGWKHEE
                     IATELGMAVGSSKAQLHRARGLLRTRIGVMA"
     gene            20505..21029
                     /locus_tag="PK399_04025"
     CDS             20505..21029
                     /locus_tag="PK399_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12322.1"
                     /translation="MNERTNNPAHDSGDAALRLALRGLRQDGDPARDLWPGIEARLHA
                     LPQSSTPAVARRRWTWPLAMAASMLLSAGLAWQMRPVETPVAVPAAETRMANTLVQRE
                     ARTLTVQYQAALRELEFQPAPANWQPGLEALDRSAAEIRSALQQNPDSRLLLERLRET
                     YTRRLALSRRALYA"
     gene            21040..21930
                     /locus_tag="PK399_04030"
     CDS             21040..21930
                     /locus_tag="PK399_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPW12323.1"
                     /translation="MRTLLIRCTLALACLAALPALAGTPIDQTRPLDARGRIEIENLK
                     GRVEVRAWDRQEVKVTGTLGDGVEKFSMDGDRRNLRIEVKYPSRSGRTEPTVLIVQVP
                     ILADLEIETVSADIDVHGIASRELSLESVSGDIAANGAPREARIESVSGDVRLTLNSP
                     DVSVDTVSGELVLQGRLNGEVSLETVSGNVRMDGLGERVRSLSAGTVSGDMDLKTALA
                     DGGEIRLESVSGDLLLRLPRDLSAEVSGESFSGDLSAPDARIQREEFGPGSSFEARYG
                     AGKGEVRMETFSGDATLRLL"
     gene            complement(22012..22848)
                     /locus_tag="PK399_04035"
     CDS             complement(22012..22848)
                     /locus_tag="PK399_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12324.1"
                     /translation="MLALACASGVAQAQSKAAKRNDAQVLPVLNKDNGKLEAVLLLEP
                     ATTGAGAGARWRFGSTTLEATYGLETGSSLALLCDRQSGLPSAIGQLASNCVLASVEG
                     DKHGNGSRHATTSASLSRNGNKVGVSVGSGRDTLPVWLSPNQSGGARYEQNDFSLFAE
                     KAISREGFVSVGGTVARARLVPAADVPQLADQWNTRSLSIGGGFGSFGANVVGRVVNV
                     PGESNVWKGLGLGLTWRTPWSGQLSVGADNVVTRGKNPFSVNGQKDDEGTVPYVRYQQ
                     DL"
     gene            complement(23009..23938)
                     /locus_tag="PK399_04040"
     CDS             complement(23009..23938)
                     /locus_tag="PK399_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HPW12325.1"
                     /translation="MTQAPDARSQDLQLRFLLPDAGVRGVAVHLDDTWAAMTERATYP
                     PAAAELLGEAAAAAALFTGHAKVDGRLSVQLRADGAIRTLFAECTAAGTLRGIVRVSD
                     AVADGRTAPPSRDLRALGDDAVLAITVENPALGNREPMRYQGLVALDAGRLDEAFEGY
                     FRQSEQLPTRLLLACDGRRAAGLMLQKLPGDEGDSDGFRRIGALFDTLASEELLALAP
                     AEVVHRLFHEEQPQLLGEKPLRFACSCSRERVESMLVSLGEPEVQAAVEAGLGEAKIH
                     CEFCGQEYRFDGDAVGILFRDAAAELPAAPGIQ"
     gene            24070..>24341
                     /locus_tag="PK399_04045"
     CDS             24070..>24341
                     /locus_tag="PK399_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPW12326.1"
                     /translation="MPRRGRWRGWWWKLPLLFAGFSMLQVLVLRFVDPPFSAFMAARQ
                     LDALVAGDTDFRIAHDWRDFERMSPNLPLALVASEDQNFGNHHGFD"
BASE COUNT         4007 a         7948 c         8238 g         4148 t
ORIGIN      
        1 aagaaggcca agccggccgt cgtcccggcg atcccggctc cggtgatctg aagtcaagcg
       61 accgacccgg gcatgcccgg gttggcgtct gcaacaccat gaagaccctt cccgttgccc
      121 aggcgggagg ggtttctttt tttagctctt cgcgcgcgtg cctggcgctc gccgcacgtg
      181 cggccgcgag tgcgaaggat cagaacggcg aggcggtggc ggccgacgcg tcggtgcccg
      241 ccggcgtttt caccggctgg gtggcataga gcgtttcgga cggctcggcg atgtcgatgt
      301 cgtcatccag ccatgtgcca tcggccaggc cgagcgcgct gtccaggatc gtcggaagca
      361 gtccgctggg cagcgcgctt tcgctgttcc acagcagcac cacgccgagg tccttgccgg
      421 gtagcagcgc catcgcgccg cgatatccct gcaccgcacc gccgtggaag accacgtcgt
      481 tgccggcata gttgtacacg cgccagccaa gcgcgtaacc cgcggaactc agtcgcgtgc
      541 gccgccacga cgcaccccgg atttcgctcg gggtatcgac cagaggcgca tgcagggtgg
      601 ccagcaaggg ggcgggcagc acgtcggggc gatgccccgt ttgcgccagc atccactgcg
      661 ccatgtcgct gatgctggcg ttgacgccgg ccgcgggcgc gacacggtaa taagtgggct
      721 tgggtgtcag cggtacccaa ccgccgccgc cgcggacgtg cgggcgcgcc cagcgtgcgc
      781 tggcctggat gccctcgagt ccgtagctcg cgtcatgcat gccgagcggc ttgaagatgc
      841 gacgcgccac ggtctcgctg aagaactggc cggtggtggc gaacaccacg tcgccgatca
      901 ggctgaaggc gacattctgg taggcatagc attcgccggg cgcgcatttc atcggcgcgt
      961 tggccagctt catggtcagg ctgtggtagt ccgcgttgcc ctcgaggtcg cggtcgaagg
     1021 cattgtgggt cagtccgacg cgatggctca gcacgtcggc gacggtgacc tgctgcgcgg
     1081 cgttcggcag cgacaggcgg aactccggga cgtaatcggt gaggtggctg tcccagcgca
     1141 gcacgccgtc gttgaccagc atgccggtga cggtgccggc gaacgacttg gacagcgagg
     1201 caaggcggaa caccgtgtgc gcgtcgaccg gttgcgggtt gcgcgcatcg gtgacgccgt
     1261 agccgcgtgc gctgaggatc tggccgtcct tgacgatggc catcgccagg ccggggatgc
     1321 ggccattggc gaccaactgg ttggcgaccg cttcgaagcg ctgcacgtcg aaaccggcgg
     1381 cgacctgctt gctgctgtcg atcggggccg gcaaccctgt attcgcctgc agcggttgtg
     1441 gcgcgcggct gaccgttccc ttccagtgca tcgcactctg cgcggaggta cccactgcga
     1501 gcggcagcaa tgcgctgacc agggcgagtc gacacgcgtg ccggatgcgg tgttctttca
     1561 tcggccatgc cccctgcgta tgctgcaacg accggtcgat tctagcgcct gtttccgcga
     1621 ttgcatgaac aaggggaact tcgatgcttg gcttgttgat tttcatcgca attctcgcgg
     1681 ccgtcgcgtt ctggggcgtg ggtatctaca acggcctggt gcgggctcgc aacgcattca
     1741 ggaatgcctt cgcgcagatc gacgtgcagt tgcagcgccg cttcgacctg gtcccgaacc
     1801 tggtcgaagt cgccaagacc tacatgagcc atgagcgcga gacgctggag gcggtgatcg
     1861 ccgcgcgttc cgccgcgcag tcgggcctgg ccgcggccaa ggccaatccc ggcgatgccg
     1921 aggcgatggc gcagctcgcc gccgcgcagg gacagctcaa tgccggcctc ggccgcctga
     1981 tgatggtggc cgaggcgtat ccggacctga aggccaacca gaccatgatg cagctcaccg
     2041 aggagctgac cagcaccgag aacaaggtgg ccttcgcacg gcaggcgttc aatgacgcgg
     2101 tgatggccta caacaacaag cgcgaggtgt tcccgtcgag catcttcgct ggcatgttca
     2161 atttcgccgc cgccaacctg ctggagatcc cggcggacag gcagtcggac gtgcgtgccg
     2221 cgcctcaagt gaagttctga tcctggatga cgcgggccgc gtggaggcgg gtcgcgtgaa
     2281 cgcggacgcc ggcgcatgaa tttcttcgaa cagcaggccg tcgcccggcg gacgtcggcg
     2341 cggttggtgt tcctgatggc ggttgcgatc gccggcatcg tgctcgcggt cgatgcggtg
     2401 gtctgggttg ccacgcagtc accggagttg gtcctgttcg ccacactgtc gtcggtcgcg
     2461 gtgatcgcgc tcgggtcgct gtaccgcatc gccagcctgg gcggtggcgg cgaaccggtt
     2521 gcgcagcaac tgggcggcac gctggtcccg gaaaacacca ccgagccggg attgcgccgc
     2581 ctgcgcaacg tggtcgagga aatcgcgatc gcctcaggcg tgccggtgcc gaaggtctac
     2641 gtgctggaac acgaagccgg catcaacgcc ttcgctgccg gctactcgac atcggacgcg
     2701 gtgatcgcgg tgacccgggg cgcgctggat cgcctcaatc gcgacgagtt gcagggcgtg
     2761 gtggcgcacg agttcagcca catcctcaac ggcgacatgc gcctcaacat ccgcctggtc
     2821 ggcgtgctgt tcggcatcct catgatcggc ctgatcggtc gcaagatcct ggagcacggc
     2881 cgtttcggcg gacgcggcaa gaacatcggc gcgatcctcg tcgccgcgct ggtggcgatg
     2941 ctgatcggct acgtcgggtt gttcttcggg cggatgatca aggccggggt cagtcgcacg
     3001 cgcgagaaac tggccgatgc cagcgcggtg cagttcaccc ggcagacgca agggctgtcc
     3061 ggcgcgctga agaagatcgg tggcctgcac gatggatcgc gactcaacca gcgcagcgat
     3121 gcggaggaag tcagccacat gctgttcggc gatggcgtgg gcttcgacca gctcttcgca
     3181 ggcaagttgt tcgccacgca tccgccgctg ctggagcgca tccgcgcgct ggagccgggg
     3241 ttcaacgatg acgcgatggc acgcctgcag gcgcaatgga gggcgtcgcc accggacggc
     3301 atgcaggaag acgtggcgct tggcctgggc ggtggcagcg gtgcgcgatt gcccggcgcg
     3361 acgcaggcgc tgagcgtgac gccgccgatg gtcgccgcgc aggtggccgc gcccggcgcg
     3421 gacgactatc gccgcgccga cagcatcgtc atcgcgattc ccgatgccct gcgcgacctc
     3481 gcccgccagc gcgaatcggt gatgccgttg ttgttgggcc tgttgctggc cggggacgaa
     3541 gcactggccg aacgccagcg cgtggacatc gccgcgcgac tgggtggcga ggtggccgac
     3601 cacgccgtgc gcatccacca gcaactcacc gcggacctgc atccgatgct gcacctgccg
     3661 ctggcgggac tggcgttccc ggtattgcgc ctgcgtccgc gcccgcaact cgatgccttc
     3721 atggacacgg tgcaggcatt ggtccatgcc gacggccagg tctcgctgtt cgaatactgc
     3781 ctgggccgcc tgctgaccgt gcaggtgcgc gagtcgctgg atccatcccg ctacgcgcgt
     3841 ttcggccggc gcaagccggg caacgtgcgc aaggagttcg ccaccctgct cgcggtggtg
     3901 gcacaagccg gccacgagga tcgcgcctcg gcgcagcatg cgtacctggc cgggctgcaa
     3961 cgcgtgctgc cgcgcgatca cttgccgtat gtgccgcccg cgaatggcgt gcttgcgctg
     4021 gatgcggtgt gggagccgct ggatgcgctc gacccgctgg ccaagcaggc gatggtcgag
     4081 gcggtgaccg ccgcggtcag ccacgacggc cgggtcagcg tggccgagtc cgaactgctg
     4141 cgcaccatct gcgccgtact gcattgcccg ttgccgccga tgctcgagcg aggttgagag
     4201 acaccactgc ccttggtgcc gtagcccggt taagcgcagc gcacccggga catcgttcga
     4261 actcagcccg cctcgatcaa cccgccttga tcaggtccgc tgcgcgttcc gcgatcatga
     4321 tcgtcggtgc attggtgttg ccgcccggca gcgccggcat caccgaggca tcgatgacgc
     4381 gcaggccttc gacgccgcgc accttcaatg cggcatcgac cactgacgcg tcatcgtcgc
     4441 ccatccggca ggtgccgacc gggtggtaga cggactccgc cttggcgcgg atgaaggcga
     4501 ccagttcggc atccgacaga tcggtgcgcg tcggatggat cggcgtaccg cgataaggat
     4561 cgaaggcggg ctgggcgaag atctcgcggc tcaccttggc gcattccagc atcaccttca
     4621 ggtcgaagcc ttcggcatcg cccaggtagt tggcgtggat gcgcggcttg tcgctggcgc
     4681 ggttgctggc cagcgtgagg cggccgcggc tgcgcgggcg caggaagcag gcatgcaggg
     4741 tatagccgtc gcccgccagg cggtggcggc cgtggtcgtc gagcatcgcc ggcacgaaat
     4801 ggaactgcac gtcgcagcgg tcgtcggtgg ccagtggcga acgccagaac ccgccggcct
     4861 cggcgaggtt gctgctgccc gcgccacgat ggccgcgcag gaaatagtcg aacgccatct
     4921 tcggctcgtt gatgcggtcg taggtgatcg gctgcgtcga atgctgcagc gtgcagatgt
     4981 cgaggtggtc ctgcaggttg ccgccgacat tggcggcatc gtggacgaca tcgatgccga
     5041 ggcggcgcag ttcatccgcc ggaccgatgc cggacagcat cagcgtctgc ggcgaattga
     5101 tcgcgcctcc gctgaggatg acctcgcgcg atgccggttg gtggaacgcc ttgccgccca
     5161 tcgcgtaggt gacgccactg gcgcgtcgtc cctcgaaggt gatccggctg accagcgcgc
     5221 cggtatgcac ggtgaggttg ggtcgcgagc gcaccggtgc caggtaggca gctgcgctgg
     5281 agcagcgtgc gccatcgcgc tgggtgacct ggtacaggcc aacgccggcc tgggtgggac
     5341 cgttgaagtc gccattgcgc gtcatgccga cctgggtggc ggcctcgatg aagacatccg
     5401 acagcgggtt gtggtggcga aggtcggaca cggccagcgg gccgtcgccg ccgtgcagcg
     5461 cgtcggcccc gcgcgtgttg ccctcggcgc gcttgaagta ggggagcacc gactgccagt
     5521 cccagccggc ggcggccttg ccccagtcgt cgtagtcgcc ggcgacgccg cggatgtagc
     5581 acatcgcgtt gatcgaactc gagccgccca gcaccttgcc tcgcggccac cacagcgtgc
     5641 ggttgtcgag gtgcggctcc ggcgcggtgt cgtaatccca gttcacgccc ttgcggttga
     5701 ccagcttggc caggccggcg ggcatgtgga tgaagggatg ccagtcgcgc gggccggctt
     5761 cgatcagcaa caccttggtg tccgggtctt cggagaggcg attggcgagt acgcagccgg
     5821 ccgagccggc gccgatgatg atgtagtcga acaacgtacc gccccgcatc gaagacccgg
     5881 cgacgctatg cccccaggct agagcaaatg ctccatgcgc tggcggcgaa aacgcgcttc
     5941 ggctaacgtg ccggcacagg gcaggaacaa cgtgcgatga cggagaccgg gacgatcagc
     6001 acggacgcgc tgccttggca tcgccgtgcc atcgcggcat tggcggaaat cctgaccctg
     6061 ccgtcgctgc ggtggtcgtt cctgttcttc ttctgcctgc tggccggcta ttacgtgttg
     6121 cgcccggtac gcgatgcgat gggggcgtcg tccgaagtcg aggcggtctt cccgtcggcg
     6181 atgatcgtgt tcttcgccgc gcatggcatc ccgttgaagg acctggtcct gcaggtgctc
     6241 gcgacctgca ccttcgcgct gatcctgctc gcgcagccgt tgtacggcgc gctggtgtcc
     6301 aggttcccgc ggcgggtgtt cctgcctgcg ctgttcggct tcttcatcgc ctgcctgttc
     6361 gtgttctacg ggttgttcca cagcaacgtc agcgggcgcg ggatggcgtt cttcctgtgg
     6421 gtcacggtct tcaacgtgtt ctccgtgtcg atcttctggt cgttcatggc cgacgtgttc
     6481 gccagcgacg aggcgcgcaa gtactacggc tatatcggtg ccgcaggcac ggtgggtgcg
     6541 ttcaccgggc cgatcctcac ccgagtgctg gccgaaaagg tgggcctggc caacctgatg
     6601 ctggtgtcgg cgggattcct gtccgtgtgc gtcgtgtgca tctggcggct gcgccgctgg
     6661 gcagtggcgc gcgaacgcaa ggccgggcgc gacaacgaaa gcgcgatggg cggcgacatc
     6721 ctggccggcc tgaaactgat cttcagggag ccgctgctgc gctggctggc cacgatggtg
     6781 ttcctggggg tcggcgtggg ccagctgctg tacaaccagc aggcggagat tgcgcgcgaa
     6841 gggttcgcca gcgcggaagc gcgcaccgcg tactacgccg gcatcgacat cgcggtgaac
     6901 gtgctcaccc tggtcgtgca actgttcgtc acccgttggc tgctgtcgcg ctggggattg
     6961 gcgccggtga tcctgatccc gatggccgcg ctgttgctgg gcttcgcggt gctggccgca
     7021 tcaccgctgc cgatggtggt ggcaaccgtg ctggtcgtga cccgttccaa cgagttctcg
     7081 ctgatgaagc ccggccggga aaccatctac acccgggtgg acaggcagtg gcgctacaag
     7141 gccggtgcgg cgatcgacac ggccttctac cgcggcggcg agatcagttt ctcctggatc
     7201 tacaaggcgt tgtcgatgtt cggttcgcac gtcgtgttcg gcgtcggcct ggccgcggcg
     7261 ggggtgatga ctgccagcgc gtggcgcctc ctgcgcgaag agaagaaact gccggacgaa
     7321 ggtccgcgaa cggagccggg atgatgtcga tgatcgcgca gctgttgatc gggctggtgg
     7381 cgctgctgca cctgtatttc ctggtgctgg agatgttcct gtggaccagg ccgcttggcc
     7441 tgaagacgtt ccgcaacacc ccggaaaagg ccgaaaccac ccgcgtgctg gcggccaacc
     7501 aggggctgta caacggcttc ctcgccgccg gcctgttcta cgccatcgcg accggctcgc
     7561 gcgagttcgc gctgttcttc ctggcctgcg tgatcgtcgc cgcgctgtac ggcgcataca
     7621 gcgtcaacaa gcgcatcttc gtcgtgcagg gcgtgccggc gctggccgcg ctggtggcgg
     7681 tcttgctgta ggtcgcgccg tcattgaagc gaggcgagcg tgccgggtgt tcgcgcagcg
     7741 gcatttcgag ccgcgaagcg aatgcctggc acgctcgcct cgcaaccgcg gaagcaaaac
     7801 tcagcgcatc gctttcggcg acacccacat cgcgatggtc gccgtgaaca cggcctcgcc
     7861 ctgcgggttc tcgaccacga cgtcgaacgg caggtcgtag ccttcgctgg cctcgcgcgg
     7921 cgggaacgcc ggggtggcgg tggcgcgcat cgtgccgacc gccttcttca ggtaagccac
     7981 ggtcattccc ttcgggatcc agcgcatcga cgccgggatg ctgacgtcgc aggccaggcc
     8041 gccgacgaat tccgcgaggt tgcacaacgc gatcgcgtgc accgtgccga ggtggttggt
     8101 gaccgcgcgc cggtggcgca gggtcgccac gccacggccg ggttccagca cgtcgatgcg
     8161 cggggcgatg ctggagaaat acggcgcctt ccagcacacc agccgcgaga aaagccagcg
     8221 acccgcgggc cactggctga tgcgccggta ggacgcgagg acgttcacgc cgccgcgcgg
     8281 gtggcgatgc gtccgtgcag cgtcgcgtaa cccgccgagc gcagttcctc cggatcgaag
     8341 tcgtcgacca tgatcgcgtc cagggtcagc gcgcgcagtt cttccaactg cttgcgctcg
     8401 tccgcggtga tccagccttc gcgcacgcct tcgtcgagct gcgcatcgaa ctcgagcgct
     8461 tcgatgtcgc tgttcttcaa cgccttgagg aacttgcgct cgaccggctc ggccatcacc
     8521 accttcggca ggtaactgac gatgcggccg cccgggttgt tcgcggtcgg ggtggtgaac
     8581 acgccttcgg ccaggcgctc gcgcgcatcg cacggcgtca tcagcaggct cgcgaccttg
     8641 tggcccaggc gatcgcttgg cgcctgcgcg cggcggcccc acgggaagat caccagccac
     8701 agcagccagc cgaccggacg gatcgggaag ttgcgcagcg caccggagag cgcagtctcg
     8761 atcttgttga ccgaatcgtg gatggcgtac gccagcaacg gctggtcgcc ggccggattg
     8821 ccgatgtcct ggtggcgctt gagcatggcg ctggcgatgt acaactggct gagcacgtcg
     8881 cccaggcggc cggacagcga ttccttgaac ttcagcttgc cgccgagcag cagcatcgag
     8941 gtgtcggcca tcaccgccag cgcggcggaa tagcgattga gcttgcggaa gtagcgacgg
     9001 gtgtcgtcat cgcccggcgc actgccgatt tgcgaaccgg tcaagccgta ccaccagctg
     9061 cgcaccgcgt tgctgatggc gaagccgatg tggccgaaca gggcagcatc gaactggtcg
     9121 atgcgttgct tcgggtcggc cagttgcgcc gacttcattt ccttcatcac ccacgggtgg
     9181 cagaggatcg cgccctggcc gaagatcatc aggctgcggg tcatgatgtt ggcgccctcg
     9241 accgtgatcg cgatcggtgc ggcctgccag gaacgcccgg cgaagttgcg tggccccagg
     9301 atgatgccct tgccgccgag gatgtccatg ctgtccatcg ccgccttgcg gcccatctcg
     9361 gtgcagtggt atttcgcgat ggtggacggc accgctgggt tttcgccgcg cgcgaccgcg
     9421 gcggcggtgg cttcggacaa ggcactggtg gcataggcat tgccggcgat gcgggccagc
     9481 gcttcttcca cgccctcgaa gcggccgacc gacaggccga actgcttgcg gatccgcgcg
     9541 tagctgccca ccgtgaccgc gcccatcttc gaaccgccgc tggcggtcga gggcagggtg
     9601 atcgaacgcc cgatcgacag gcattccatc agcatgcgcc agccctggcc ggcatagtct
     9661 tcgccaccga tcagctgcga caggggcacg aacacgtcct tgccgtggat cgggccgttc
     9721 tggaacggcg agttgagcgg gaagtggcgg cggccgatgt ccaggcccgg cgtgtcgcgg
     9781 ggcaccagcg ccagcgagat gccgatgtcc ttcttgtcgc ccagcaaccc gtcggggtcg
     9841 tacatgcgga acgcgacgcc gatcagcgtg gccaccggcg ccagggtgat gtaacgcttg
     9901 ttgagcgtca gcttgatgcc cagcacgttg atgccgttcc actcgcccat gcagacgatg
     9961 ccgtagtccg ggatcgaggt ggcgtcggag ccggcgaacg gcccggtcag cgcgaagcac
    10021 ggcacgtcgc ggccatcggc gaggcgcggc aggtactgct gcttctgttc ctcggtgccg
    10081 tagtgcatca gcaattcggc cgggcccagc gagttcggca cgcccacggt cgaactgacc
    10141 acgctgctga tcgaggccag cttctggatg accttgtggt tcatcagcgc ggagaaaccc
    10201 aggccgccgt attccttcgg gatgttcagg ccgaagaacc cgttgcgctt gatgtagtcc
    10261 cagagctccg gcggcaggtc ggcgcggaca tggcagatgt cccagtcgtc ggtcatccgg
    10321 cacagttctt cgcacggacc atcgaggaag gcttgctcct ctgcggtgag ctgcggcttc
    10381 ggcagggtca gcaactggtc ccagtccggc ttgccggaga acagctggcc ctcgaaaccg
    10441 accgtgccgg attccagcgc ggtgcgctcg gtatcggaca ggggcggcaa cagcttggtg
    10501 tagaagccca gcagcggcgc ggtgatgaag ggcttgcgca gttgcggcac cagcagcggc
    10561 agtgcgatca gcaccaccag cacgccggcg acgatcgtcg ccacgtggtt ggcgcccagc
    10621 agccagcagc cgaccagcag ggtcgcggtc atcgcggccc agaccgccag acgcaggcga
    10681 tggtaggcgg cgaacatgcc gctggcgaag aaggcgagga agggcagggc gatgctcatg
    10741 tgggactccg tgtagcgcgg atgagtcggg cgatgcggca ttgcattgcg gatgcggctt
    10801 cgcctaccat acggccaagt atggccagtc cggcaagcct gtcaatcgca ggaaaagagc
    10861 aattgctcga catactcccg catccgcaag cgtacggata gaatgcccag atgaccgaag
    10921 ccgcatcacg caccgggcgc ctgagcgccg atgactgggc ccgcgaggcg ctggaccaga
    10981 ttgccgaaca gggcgtggcg tcggttgcag tagagccgct tgcgcgtcgg cttggcgtga
    11041 ccaagggcag tttctactgg catttcccgt cgcgggatgc gttgctccag gccgcgctgg
    11101 agcgctggga aaccgccgag caggaaatcg tgttcggctc ggtggagaac gtctccgatc
    11161 cgcgcgagcg cctgcgctcg ctgttccagc tggtcgcgca cgaggtcaag tcgcacatta
    11221 tatatagcga gctgctgaag gccctcgacc atcccacggt cagcccggtg atcgggcggg
    11281 tttcccagcg acgcctggac taccttaccg cgagcttccg gcaggccgga cttggccgca
    11341 ccgcggccca gcatcgcgcg cgcctggcct acgcggccta tgtcggcttc ctgcagctca
    11401 gcctgcagtt gcaacagccg cgcctgcacc acgacgagtt cgaggcgtac gtcgagcacg
    11461 tcatggatac cctgatcccg caaacctgat tcccgcgcgt cgcgcggcag gtttccatcg
    11521 cccgcgattg caatcgattt cactctgaac agtttcacga ggaaccatcg atgagcagca
    11581 ggctcgatgc attgaaccaa tgggtgctgg acgttgcacg gctgacccgg cccgaggcga
    11641 tccagtggtg cgacggcagc gacagcgagt acgacgcgct ggtcgcgaag atgcaggacg
    11701 acggtacgct gttgccgctc aatcccgaca cccatcccaa cagctggctg caccgctcgc
    11761 atccggacga cgtcgcccgc gtcgagcacc tgaccttcgt gtgcacgccc gagcaggagg
    11821 atgccggtcc gaacaaccac tggatgtcgc cggccgatgg ccacgcacgg atggatgcct
    11881 tgttcgacgg ctgcatgcaa ggccgcacga tgtatgtcat cccctattgc atgggcccga
    11941 tcgattcgcc gttgtcgcgg tgcggcgtgg agatcaccga ctcgccgtac gtggtcgcca
    12001 acatgcggat catgacccgc atgggcgccg cggcactcgc gcgcatcgaa cgcgaaggct
    12061 cgttcgtgcg tggcctgcac tcgatcggcg aactggaccc ggaccgacgc ttcatcatgc
    12121 atttccccga ggagttggcg atcaagtcct acggttccgg ctacggcggc aacgcgctgc
    12181 tcggcaagaa atgccatgcg ctgcgcatcg cgtcgcacca ggcgaggcag gaaggatggc
    12241 tggccgagca catgctgatc ctcggcctgg agaatccgca gggacagacg cattacatcg
    12301 ccgcggcgtt cccgagcgcc tgcggcaaga ccaacctggc gatgctgatc ccgcccgagg
    12361 cctatcgcga agccggctgg aaagtgtgga cggtcggcga tgacatctgc tggatgcgcc
    12421 ccggcgccga tggccgcctg tacgcgatta acccggaggc cggcttcttc ggcgtcgcac
    12481 ccggcacctc tgcgaaatcc aacccgaatg cgttggcctc gatccagtcg aacaccattt
    12541 tcaccaatgt cggtaccacc gccgacaacc agccatggtg ggaaggcatc gacaacgacc
    12601 agcaccccgc caccgactgg cgcggcgatc cgtacgatcc ctcggtgcga ccggccgcgc
    12661 atcccaacgc gcgcttcacc gtcagtgcaa ggcaatgccc gtcgtattcg tcgatggccg
    12721 aggatccgca gggcgtgccg atctccgcga tcgtcttcgg cggccgccgc gcttcactgg
    12781 tgccgctggt gttcgaggcg cgcgactgga cgcatggcgt gctggtcggc gcgtcgatgg
    12841 gatcggagac cacggccgcc gcggccgggc aggtcggcgt gatgcgccgc gatccgatgg
    12901 cgatgaagcc cttctgcggc tacaacttcg ccgactactt cgcccactgg ctgtcgttcg
    12961 acaagccggg cgcgaaattg ccgaaagtct tccacgtcaa ctggttccgc aaggggcccg
    13021 atggcaggtt cctgtggccg ggcttcggcg acaacctgcg cgtcctggag tggatgatcg
    13081 gacgcgtcga gggcaccgcc ggcgcaatcg agacgccgat cggcctgcaa ccggccgagg
    13141 gcgacatcaa cctgggcgga gtggacctgg gcgaagacgc gcggtcgctg ctgttcggat
    13201 tcgatcgcgc tggctggcag tccgagttcg aaggcatccg tacctacctg cacgactacg
    13261 gtccccgcat gccacctccg ctcctccagg agcaggcgcg catcgagaac gcgctcggtt
    13321 agggaggaag gcctagtaca tcgagattgc ggcaacgcaa catgttttct ttcgaaactg
    13381 aatgggcaga aaggaacatg aattgcggat gaggggcttg ttaaggattt gttcgctggt
    13441 ctatagtccg tcggtcagcc cgcctcgggc cagacgtaca tatgtatttt tgtcacttct
    13501 gtccctatgg gggtttacac aatggcttct aattatttcg cgaaaggcct gaagcagagc
    13561 gcgctcacca tcgcgctcgg catgtgcttc gttggcggcg tccaggcgca gaccaacacc
    13621 ggcggtaacg tggtcggtag cgccgcagct ggcgacacca tcaccatcac cagcccgtcc
    13681 accggcttca gccgcaccat caccgcgggc gccgacggca acttccgctt cgcgcagctg
    13741 ccttccggcc agtaccagat cagccagaac ggcggtgccc cgcgcaccgt gaacgtcaac
    13801 gtcggtacct cggcgaacgt cgatttcgcg ggcgctgcaa ccaccctcga taccgtgacc
    13861 gtgaccgcca ccagcggtgc ggtgaacccg atcgacgtct cctcggtcga gtccaccacc
    13921 atcctgacgg ccgagcaggt cgccaagatc ccggtgccgc gcaacatcac ctcggtcgcc
    13981 ctgctggcgc cgggtaccgt ccgtggtgcc gcgaatgccg gcttcggcgc gctgccgtcg
    14041 ttcggtggtt cgtcggtcgc ggagaaccag ttctacgtca acggcttcaa catcaccaac
    14101 accttcaaga acctcgcgtt cgcgcaggtc ccgttcgaag gcatcgccga atcgcaggtc
    14161 aagaccggcg gctacggcgc ggaattcggt cgttcgaccg gcggcgtgat caacatgatc
    14221 accaagcgcg gtaccaacga gttcaaggcc ggcggcagca tctactacat cccggaatcg
    14281 ctctccgaga ccagcccgga cctgtactac aacaacggcc tgtgggtttc caacaactcg
    14341 aacgacaacg gttgggaagc caccgccgcc atctgggcgg gtggtgcgct gatcaaggac
    14401 aagctgttcg cctacggcct gatccagtac ggcaagaccg caagcgagac ctacaccagc
    14461 cgtttcggca acaccaacct ggacagcagc agcaagcgtc cgcagtggtt ggccaaggtc
    14521 gactggcaga tcagcgatga ccacagcgtc gaattgaccg cgttctccga caagcgcgag
    14581 ttcaccgacg actactaccg cgcgtcgttc ccggacagca ccaccaaccc gaccacgggc
    14641 gcgatcacgg ttggcgccaa cccgaccacc gtgttcggtg actatcgcgg caccgacttc
    14701 aacgaagccg gtggttcgag ctgggtgctc aagtacaccg gctacctgac cgacaacttc
    14761 accctgtcgg ccctgtacgg ccacggcgag ttcagccgtt ccaactacgg tgtcgccgcc
    14821 aacggccagg tgcaggaata caccggtaac ctgaccggtc cggtcgcggg ctgcccgatc
    14881 atcagcgact cgcgcgcggg cgtttcgacc ggcgccgtgc cggcgatccg cggttgctcg
    14941 ttcatcaaca ccctcggccg tcgcgacgcg ctggacgagc gtgaccagtg gcgtatcgac
    15001 gccgaatggc agctgggcga ccacctgctg cgcttcggta tcgacaacga caagttccgt
    15061 tccgtcgatg gttccgccta cagcggcggc gccaactacg tctaccagtc ctgcaacgcc
    15121 acggcggcga cctgcggtcc gaacggcttc cgcacgcgtt atcgcagctt ccgctccggt
    15181 gccaacgttg gcgttgacca gcaggcctac tacatcgaag acagctggag catgaccgac
    15241 aacttcgtcg cgtacctcgg cctgcgctgg gataccttcg agaacctgaa cggcgaaggc
    15301 gagaagtacg tcaccatcaa gaaccagttc gccccgcgcc tgggcttcag ctgggacgtg
    15361 ttcggtgact cgaccttcaa ggtcttcggt aacgctggcc gttacgcgct gccgctgacc
    15421 gcaggcgtcg cgacccgcgc ggcctcggcc tcgctgtacg aagagcgcta ctactcctac
    15481 accggcgtcg atccggccac cggcaccccg accggcctga ccccgatcgt caacccgtcc
    15541 accggcagca gcgcggttcg ctacctgaac ggcgaagacg gccgtccgaa gttcgccgcc
    15601 ggttacgcgg tccagaacct caagccgatg taccaggatg agttcatctt gggcatgcag
    15661 aagcagctga ccgacaactt ctcgctgggt gttcgcgcga tccatcgcga cctcggtgcc
    15721 gcgatcgacg ataactgcga ttacgcgacc ctgttcgagt acgtggaagg caatggtccg
    15781 ggcacgaagt tcaactattg cttcctgttc aacccgggtg aagatgcgga aatcctggcg
    15841 gactggaacg gcgatggcac ggcgaccgtg aagaccatcc cgggtgcgga cctgtcgccg
    15901 aaggccaagc gcaagtacac cgcgctggag ttcttcgtcc agggcaactg ggacaagttc
    15961 ttcctgcagg gttcgtacac ctgggcgaag tcgatcggca acaccgaagg tggcgtgcgc
    16021 tcggataacg gccaagccga caccggcacc acctcggact tcgactacgc ggctgcgatg
    16081 atcggtgcgt acggctacac gccgaacgat cgccgccact cgctgaagct gttcggcaac
    16141 tgggacatca ccgaccagtg gtcgttgggt gccaacctgc tcgtgcagtc cggccgcccg
    16201 atcagctgca tcggcgttta ccagggcctg gatgtcagcc attacggcaa catccacttc
    16261 tcctgcgacc cgggtgcgcc tgatggcacc ggcaacaacg gcagcacgat catcgggcgt
    16321 ggtaccgtcg gccgcacgcc gtggaccacc aacctcgacc tgaacgtggc gtacaagccg
    16381 tcgtgggcgg aaggcctgca gttcaaggtg gacgtgttca acgtcctcaa cagcaccaag
    16441 gtgatctcgg tcaacgagat cggcgaggat gcgagcggca acccgggtcc ggtggccaac
    16501 acctacctga ccccgaccgc gtggcagtcc ccgcgccagg tgcgtttcct ggtgcagtac
    16561 gacttctaag cctcatcgct tcattgcagt gattgagcgg ccgccttcgg gcggccgctt
    16621 ttttttgcta acgtccgccg tggccttcga tgccacgcga cggtctcctg catgactccc
    16681 gaacaccttg aggcactctg gcgcactggc cagcagcagt ttgcgcaggg cctcctggca
    16741 gaggcccgtg cgacctatac ccggatcctt gccggcagcc cgcgccaggt catggtgcag
    16801 atcgcgcttt ccgagttgga acagcgtgcc ggctgctacc gcgccgcgcg cctgcatgcg
    16861 ctgcaggctg ccgacaccgt ggcgcagacc aagcgctggg aaggcttgtc ccatgtcacc
    16921 gcgaacctgc tgtcgttcga cgaacgcgaa cacgtgctgc acttgatcca ccaggcggac
    16981 tggaacgatg tacgcgtcct gcgccagtcg ccggtggtct cgcagcagct ctggctgtgc
    17041 ggcgaccagg aagctgcgct gcgcttcctc gacatcgccc agaaacgcgt caagagcgac
    17101 catcggctcg cctactcgcg tgcgatggcg ctgcaacatc tcggccgcat cgagcaggcc
    17161 tcgtccgcat tcgaggacag cattcgcatc gctcccgact tcgcactggc gcattggtca
    17221 ctggcctatc acgcccgaag tcgtcccgcc ggtgccaggc ttgcacggct gcgtgctgcc
    17281 gctcgccatg tcacatcgcc acaggagcgg gcgatgctcc actacgcgct atacaaggaa
    17341 ctcgatgccg ccggtgactg cgatgcggct tgggccgaac tcgaagcggg tgcgtcgctg
    17401 atgcgcgaca tgctgggcta cgcgccgcgc aaattcgaaa gcgacgtgga tgcgatgctg
    17461 gatgccgagg tggatgcgat gctggatgcc gaggcagacg cgccgttgct gcccgggccg
    17521 gcagtagagg gtgcacgcac gcccgtgttc gtggtgggca tgccgaggac ggggaccacg
    17581 ttgttgaccc gcatcctcgg cgcgcatccc gcggtcgcgg acgcagatga gctcaatgcg
    17641 ctcgaacatg cgttcgcgga gaacgtggat cggttcgtgg acctgccggt ggcgcgtgac
    17701 ctgctccccc tgctgcgcgc cgccgaccag cttggcatcg cgcaggccta tcaccgtcgc
    17761 acgcaggcac ggtacaccgc cgcaacgacg cacctgatcg acaagaaccc gatgaacctg
    17821 ttcgccgccg gaatgattgc gcgcgcgatg ccgaatgcga agatcctgtg cctcgtccgg
    17881 ggggcgatgg acgcgtgcta ttcgaacctc cgccaactgt tccagaatgg cgcgttcccg
    17941 tacagctacg accaggtgca actggcggaa cgctacgcgg tcttccgccg ggtggtcgac
    18001 cgcttcgcgc aacgactgcc cgggaatttc ctcgccgtgt cgtacgagga cctcgtgcgc
    18061 gatcccgtcg agaccgggcg gcttgcgatg gaattctgcg ggctcgggtt cgaccctgcc
    18121 tatgcggaca ccactcgcaa cagcagccca tcgggcaccg ccagtgccag ccagatccgc
    18181 gaacccatcc acacccacgg catcggcgaa tggcggcgct acgaaacccg cctgcgcccg
    18241 ctcgcgaccc gccttgccga actcggcatc gcatcgtgaa cggacggatc gaactgccgt
    18301 gaccctggca agtctgcagg cacgcgtgac ggcggctgta caggcgaagg actggcccgt
    18361 ggcggcagag gccttgcgtg gcgtgacgcg gcttgcgcca cattcggcgc gggcatggat
    18421 cgacctgtca tacgtcgaat cgttccgtgg gcgctatcgt gcggcgcatg aggcgatcct
    18481 ttctgcggcg aatgcaccgc aacgcacgaa cgaggatctt gccgacctga tcgccaggct
    18541 tcgcacgttc aatgacgcgg cgggcgtgca ggcgatcgcg cgggcgctga ttggtgcgtc
    18601 cgacagtgat gcgcgactgc tcgcggcatg cgccctgcaa ttgagcaacc tcaatgacca
    18661 tgggctcgcc ctcgcatgcg cgcaggcggc ggtctcccgg caaccggccg acatcgcggc
    18721 acgactggca cgtggccaga tgctggcgca gcatggcgac atcgagggcg cggccgcgga
    18781 tttccaggct tgcctgcggc aggtgcccgg gttggcgcag gcgtggtggt cgctgtcgcg
    18841 attgcgcaag caatcgccag aggaaaacca tgtcgtgcca ttgcgccgat tgctggcgac
    18901 gccgcgcttg cggccgctgg acgccgcttt cgcagcattt gccttgcaca aggagctcga
    18961 cgaccttggg gaccatgatg gcgcatgggg tgcgctcgag caggcatgcc gggccaagcg
    19021 atccacgttg agttacgccg ctggcgacag ccgcgcactg gtcgatgcgc tcagcgcaat
    19081 gccgaccacg accacgacca cgaccacggt cgcgcacctg caggcgaatg ccaggacgcc
    19141 ggtcttcatc gtcggcatgc atcgctccgg cacgaccctg ctggaacaac tgctggacgg
    19201 cagtccgcag gtgcgcggcg tcggcgagtt gtacgacttc accagtgcga tgcgccatgc
    19261 caccgaccac cattgcaagg gcgtgatcga cgccaccatc gtccagcgcg ccgccgaagt
    19321 ggatttcgcc caggtcggcc agcgttacct cgacggaatg gcgtggcggc tcggcgacca
    19381 gccgttcttc accgacaagc tgccgtcgaa tttcctcaat atcggcttca tatgccgcgc
    19441 gctgccgcag gccaggatcc tgcacatggt gcgcgacccg gtcgaaacct gtttctccaa
    19501 cctgcgcgaa ctgttctcgg aagcgaatcc gtattcctac gaccaggccg aactggcgga
    19561 ctacttcctg caataccggc ggttgatggc gtactggcat tccgcatggc ccggtcggat
    19621 cctcgatgtc gactacgcgc agctgacccg cgatcccgag aaggtgatgc gccaggtcgc
    19681 ggcgttctgc gggatcgaat acatggatgc gatgcgcgat ccccgcagca gcacgcgcgc
    19741 ggtggccacc gcaagcgtgg tgcaggtgcg cgaacgggtg gtacggcgcg aggtcccgaa
    19801 atgggcacct tatgcgcggc acctgcaacc gctgatcgcc gccctgcggc agggcggtgt
    19861 cgaggttgcc gaggcattcg cttaaaccct ccggcacccg cgggcatcac aatggatatg
    19921 ctgatgtcga ccatgcccct tccgcaggac ttgcaggatc ccgcccaggc cgccgcggag
    19981 gaagacgcgg cgctggcgcg gcgcgcggcg gtcgcggatg tgcgtgcctt cgaacagttg
    20041 taccggcgcc accaccggcg catccacggg gtgatcgcgc ggctcgtggg ccatgccggc
    20101 gagcgcgcgg aggacctgac ccaggaagcg ttcgtgcgcg cgtggcaggc attgccggcg
    20161 ttccgcttcg aaagcgcggt gtcgacctgg ctgcatcggt tggcggtgaa caccgcgctg
    20221 atggaattgc gctcgcgccg cagccggcca tggccggagg acgacgacga ggcgctggag
    20281 tcggttgcca cgcccgatac ggccgggcgg gcgatgctcg gccgcgacct ggaacgggcc
    20341 gtggcgacgc tgccgccgcg cgcgcgcgcg gtgcttgtcc tgcacgacgt ggaaggctgg
    20401 aagcacgagg aaatcgcgac ggagctgggc atggcagtcg gcagttccaa ggcacagttg
    20461 catcgcgcgc gcggcctgct gcgcacgcgg atcggagtga tggcatgaac gagcgaacca
    20521 acaaccccgc gcatgacagc ggcgatgccg cattgcgcct tgccctgcgc ggactgcggc
    20581 aggacgggga tccggcgcga gacctctggc ctggcatcga agcacgtctt catgcgttgc
    20641 cgcagtcgtc cacgcccgcc gtggcgcgca ggcgctggac atggcccttg gcgatggcgg
    20701 cctcgatgct gctgtcggcg ggcttggctt ggcagatgcg gccggtcgag acacctgtcg
    20761 cggtgccggc cgccgagacc cgcatggcga acacgctggt acagcgcgag gcccgtacgc
    20821 tgaccgtcca gtaccaggcg gcactgcgcg aactggagtt ccagccggcc cccgcgaact
    20881 ggcagccggg gctggaagcg ctcgaccgca gcgcggccga gatccgcagc gccctgcagc
    20941 agaaccccga ttcgcgattg ctgctggagc ggctgcgcga gacctatacc cgccggctcg
    21001 ccctgtcgcg ccgcgcgctc tacgcttaag gaatccacca tgcgcacctt gttgatccgt
    21061 tgcaccctgg cgcttgcttg cctggccgcg ttgccggcgc tggcaggcac tcccatcgac
    21121 cagacccgcc cgctggatgc gcgtggccgg atcgagatcg aaaacctcaa gggacgcgtg
    21181 gaagtgcgtg cctgggacag gcaggaagtg aaggtcaccg gcaccctggg ggacggcgtc
    21241 gagaagttct cgatggacgg cgatcgccgc aacctgcgca tcgaggtgaa atatccgtcg
    21301 cgttcgggtc gcactgaacc gacggtgctg atcgtgcagg tgcccatcct ggccgacctg
    21361 gaaatcgaaa ccgtgtccgc ggacatcgac gtgcacggca tcgcatcgcg cgaactctcg
    21421 ctggaatcgg tgagcggcga catcgccgcg aacggggcac cgcgcgaggc gaggatcgaa
    21481 tcggtcagcg gtgatgtccg gttgaccctc aacagccccg atgtgtcggt ggacaccgtc
    21541 agcggcgagt tggtcctgca agggcgtctc aatggcgagg tctcgctgga gacggtgtcc
    21601 gggaacgtgc ggatggacgg cctgggcgaa cgtgttcgca gcctgtcggc ggggaccgtc
    21661 tccggcgaca tggacctgaa gaccgcattg gccgatggcg gcgagatccg gctggaaagc
    21721 gtcagtggcg acctcttgtt gcgacttccg cgcgacttgt ccgccgaggt cagcggggag
    21781 agcttcagtg gcgacctgag tgcaccggat gccaggatcc agcgtgagga attcggcccg
    21841 gggtcgagct tcgaagcccg ttatggtgcg ggcaagggcg aggtgcggat ggagaccttc
    21901 tcaggcgatg cgaccttgcg actgctgtaa tcggtcgttg cgaaaaagac gaaggccgca
    21961 tcctgcgatg cggcctttcg cttgcgtgga gcgcgccagt ggcgcttgcc ctcagagatc
    22021 ctgctggtag cgcacgtagg gcacggtgcc ctcgtcgtcc ttctggccat tcaccgagaa
    22081 cggattcttg ccgcgggtga ccacgttatc tgcacccacg ctgagctggc cgctccatgg
    22141 cgtgcgccag gtcaggccca ggccaaggcc cttccagaca ttgctttcgc ccggcacgtt
    22201 gactacccgg ccaaccacgt tggcgccgaa ggaaccgaag ccgccgccaa tgctgaggct
    22261 gcgggtgttc cactggtcgg caagctgggg aacgtcggcg gcgggcacca ggcgcgcgcg
    22321 ggcgacggta ccaccgacgc tgacgaaacc ctcgcggctg atcgcctttt cggcgaacag
    22381 cgagaaatcg ttctgttcgt aacgcgcgcc acccgactgg ttcggggaaa gccagacagg
    22441 cagggtgtcg cggccgctgc cgaccgagac acccaccttg ttgccgttac ggctgaggct
    22501 cgcgctggtg gtggcatggc ggctgccgtt gccgtgcttg tcgccttcga ccgaggccag
    22561 cacgcagttg ctggcgagct gcccgatcgc ggacggcaag ccggactggc ggtcgcacag
    22621 caacgcaagc gaacttccgg tttcaaggcc gtaagtggct tccagcgtgg tactgccgaa
    22681 acgccagcgc gcacctgcgc cagccccggt ggtggcgggc tcgagcagca ggacggcttc
    22741 cagcttgcca ttgtccttgt tcaggacggg caacacctgt gcgtcgttgc gtttggcggc
    22801 cttcgattgc gcctgcgcaa cgccgctggc acaggccagg gcgagcaggg caatagcaat
    22861 ggcagtcgga cgcgtgcgca taggtgtttg atggacaggc cgtggccggc gaagttcccc
    22921 tcccaaggac gctcgatcct agcggattta tgattcttta acaatgcccc tgcaggtcgc
    22981 aaactgccgc agcctgcctt gcttcaggtt actggatacc gggcgcggcg gggagctcgg
    23041 cggcggcatc gcggaacaga atgccaaccg cgtcaccatc gaaacgatat tcctgcccac
    23101 agaactcgca atggatcttg gcctcgccga gcccggcctc gaccgccgcc tgcacttccg
    23161 gctcccccag cgagaccagc atggactcga cgcgttcgcg cgagcaggag caggcgaagc
    23221 gcagcggctt ttcgccgagc aactgcggct gctcctcgtg gaagagccga tgcacgacct
    23281 cggcgggtgc gagagcgagc agttcttcgc tggcgagggt gtcgaacagt gcgccgatcc
    23341 ggcggaagcc gtccgagtcg ccctcgtcgc cggggagctt ctgcagcatc aggccggcgg
    23401 cgcgacggcc gtcgcaggcg agcagcaggc gggtgggcaa ctgctcggac tggcggaaat
    23461 agccctcgaa cgcctcgtcg aggcggccgg catccagcgc caccaagccc tggtagcgca
    23521 tcggttcgcg gttgccgagg gccgggtttt cgacggtgat cgccagtacc gcatcgtcgc
    23581 cgagtgcgcg taggtcccgc gatggcggtg cagtgcggcc gtcggccacg gcgtccgaca
    23641 cccgcacgat gccgcgcagg gtgccggccg cggtgcactc ggcgaacagg gtgcggatcg
    23701 cgccatcggc gcgcagttgc acggacagcc gcccgtcgac cttggcgtgg ccggtgaaca
    23761 gcgccgctgc cgccgccgct tcgccgagca gttccgccgc ggcaggtggg taggtcgcgc
    23821 gttccgtcat cgcggcccaa gtgtcgtcca ggtggacggc gacgccgcgc accccggcat
    23881 cgggaaggag aaagcggagc tggaggtcct gcgatcgcgc gtcgggcgcc tgggtcatgt
    23941 cggtatcgtg tggctccggc ctgcggataa tgcgcggcgg cggggcattc aagagggaat
    24001 ggtcgatggt aatggggcgt gtggggatgg atcgcaaggt ggccgccgat gggcgctgag
    24061 gccggaggcg tgccacggag gggtcgctgg cgcggctggt ggtggaagct gccgctgctt
    24121 ttcgccggct tcagcatgtt gcaggtgctg gtcttgcgct tcgtcgatcc gccgttttcg
    24181 gcgttcatgg ccgcgcgcca actggatgcg ctggtggcgg gcgataccga cttccgcatc
    24241 gcccacgact ggcgggattt cgagcggatg tcgccgaacc tgccgctggc gctggtggca
    24301 tcggaggacc agaacttcgg taaccaccac ggcttcgatt t
//
LOCUS       DAOOCX010000026        24336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1744_length_24336_cov_8.284585, whole genome shotgun sequence.
ACCESSION   DAOOCX010000026 DAOOCX010000000
VERSION     DAOOCX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 24336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24336
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1744_length_24336_cov_8.284585"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /gene="nadD"
                     /locus_tag="PK399_04050"
     CDS             complement(<1..609)
                     /gene="nadD"
                     /locus_tag="PK399_04050"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000839.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPW12327.1"
                     /translation="MNRPPLRIFYGGTFDPVHDGHVAIARTARDALQAEVALLPARDP
                     PHKAVTGADADQRAEMLELAIAGEPGLFVDRRELHRAGPSYSVDTLRELRSQYGADAP
                     LAWLVGADSLRQLHTWHRWRDLCEHAHLLVVQRPGADIETAALHAIAPEVAALVGERL
                     RPVSALPLSPHGGIAILPLPGLRPESSTELRRRIARGEPWAGW"
     gene            complement(606..1655)
                     /gene="holA"
                     /locus_tag="PK399_04055"
     CDS             complement(606..1655)
                     /gene="holA"
                     /locus_tag="PK399_04055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPW12328.1"
                     /translation="MELNVDRFAAQLAGEPLRPAYLVAGSETLLVLEAADAVRAAARA
                     QGIDEREVHDMEGRDADWDALDAAINAPSLFASRRLIEIRLPTGKPGTEGAKLIAGLC
                     ASPPSDVVVLVVAGDWSRSHGGKWSEAITRAGHACIAWPIKPHELTGWIGQRLRSRGV
                     KAAPDAVARLAQRVEGNLLAAAQEVDKLALLLDRDAGAPAVLDVAKMESLVADSARFD
                     VFRLTDAALNGQSAQVSRMLAGLRAEGEAIPALMGMVVKELNTVAALARARNLAAEFK
                     AQRVWESKQAVYTRALKRHPPERWQRMLVEAGKVDRISKGRVRLGEESADAWQQLERL
                     LLAVAEPGAVRLLAS"
     assembly_gap    1658..1757
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1758..2268)
                     /gene="lptE"
                     /locus_tag="PK399_04060"
     CDS             complement(<1758..2268)
                     /gene="lptE"
                     /locus_tag="PK399_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HPW12329.1"
                     /translation="MTRRLALLALSAALLTGCGFHLRNALNLPENLGPVRVLASDPYS
                     PLGELLADGLKRAGAQAAPADASGDVATLQVISEQWADTPISHDQFGRAQEYSLRYAV
                     IFSMRRADGSSAVPQQAVELARDYLAPPVDSIGKASERELLVRELQRDMAAAILRRVD
                     AASKPAAVAQ"
     gene            complement(2310..5048)
                     /gene="leuS"
                     /locus_tag="PK399_04065"
     CDS             complement(2310..5048)
                     /gene="leuS"
                     /locus_tag="PK399_04065"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPW12330.1"
                     /translation="MSEPVSYDPRTVESAAQSFWASTRAFEVSEQSDKPKYYCLSMLP
                     YPSGALHVGHVRNYTIGDVISRHKRMTGHNVLQPMGWDAFGLPAENAAIKNKTAPAKW
                     TYANIEHMRAQLKSLGYAIDWSREFATCKPDYYMHEQRMFVRLLKKGVAYRKNSVVNW
                     DPVDQTVLANEQVIDGRGWRTGALVEKREIPQWFLRITDYAQQLLDELHTLDGWPDSV
                     KTMQRNWIGRSEGLEIQFRIDGEAEPLTVFTTRPDTLMGVTFISIAGEHPLALKAAAG
                     NPELAAFLADLRQGGVSEAELETQEKRGMATGLWAIHPVTGEDVPVYVANFVLMGYGT
                     GAVMAVPAHDQRDWEFAKAYGLPVKPVIVDAAVRDALQEMSSHVAEHDDPMAVALGGH
                     AAIDVVEIDAAVAVVREYFRRIDEEGAQTERGWLINSGDYNGMDFDAAFDALASKFEA
                     EGTGARRVNFRLRDWGVSRQRYWGCPIPVIHCAACGSVPVPEEQLPVLLPEDVADAFA
                     SGDVHSPIKSDPEWRKTTCPQCGGAAERETDTFDTFMESSWYYARYTSPGATDMVDER
                     ANYWLPVDQYIGGIEHAILHLMYFRFYHKLLRDAGLVGSDEPATRLLTQGMVIADTFY
                     REDEHGAKEWINPADVELRHDERMRVTGAISRADGLPVLIGGTEKMSKSKNNGVDPQV
                     LVDKYGADTVRLFSMFAAPPEQSLEWNESGVEGMARFLRRLWRDVHAHVAQPDHPEVD
                     AGALDPAQKTLRRHIHEAIQKVGDDYGRRHSFNTAIAALMELLNHVGKFNDMSDMGRA
                     VRHEAFEAIVLLLNPITPHISHALWQALGHAETLLEDVPFPNADPAALVRDALTLAVQ
                     VNGKLRGTIEVVPDAAREVVEAAALADPHVAAFVAGKEIRKVVVVPGKIVNIVVAG"
     gene            5145..6452
                     /locus_tag="PK399_04070"
     CDS             5145..6452
                     /locus_tag="PK399_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW12331.1"
                     /translation="MRKQLLAAVVLTTLCVPAFAADAPGAGTALHCGKLFDSRSGKLL
                     GPHTVVVRNGKVADVLAGRVEAVAEATAIDLSDRTCMAGWTDLHVHLGSQSSPQSYSE
                     GFRLDDVDFAFRSVGYAEKTLLAGFTSVRDLGGDVTPHLRDAINQGLLKGPRIWAAGK
                     SIATTGGHADPSNGLNDRLAHLIGPPGPTEGVINSVDDARQAVRQRYKDGSDVIKITA
                     TGGVLSYAKSGDAPQFTVDEVKAIVDTARDYGYRVAAHAHGEEGMYRAVAGGVTSIEH
                     GSYMSERVMTLMKEKGTWYVPTLYAGRFVADKAKIDGYFPEIVRPKAARIGAQIQDTA
                     AKAYRSGVKIAFGTDMGVGPHGDNAREFQYMTEAGIPAAYALQAATFLAAEVLGVDDQ
                     GVVEVGKRADIIAMPGDPLADIGNTMKVDFVMKDGVVYRTPQR"
     gene            6449..6700
                     /locus_tag="PK399_04075"
     CDS             6449..6700
                     /locus_tag="PK399_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HPW12332.1"
                     /translation="MNLEWLGFLAAACTTLSFVPQAAKTIRTRDTSGISLWMYVVFTF
                     GIACWFGYGVYLMSWPMILSNAVTFVLSSTILGMKLKYG"
     gene            complement(6687..7268)
                     /locus_tag="PK399_04080"
     CDS             complement(6687..7268)
                     /locus_tag="PK399_04080"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HPW12333.1"
                     /translation="MKSLAGCAAWGAAGLFVIALLLASVGVPEYSHRVHPVALRGTAG
                     LPWAFAFNLVAFVVPGALLAVAGVPLRNALREGGWAARIGLVLVQFSALAFAAQGLLP
                     LDPGDADSHASRLHVLAWMLWWIAFVPGALLLAAGARRGIGFALGSVAIGLMVPIIAV
                     LAPIGLWVGIAQRLAFALWFGWWLLAAWRLSRT"
     gene            complement(7265..8170)
                     /locus_tag="PK399_04085"
     CDS             complement(7265..8170)
                     /locus_tag="PK399_04085"
                     /inference="COORDINATES: protein motif:HMM:NF020416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HPW12334.1"
                     /translation="MSKATGTGMRVVKWTLGIVVALALLVAAWMWWVVERFDTETLPP
                     RHGQVDVELFARDGGKRPLIVGLGGGEGGNAWASARWTPQRERFLDQGYALLALGYFG
                     TPNSPETLDRISLDGVHAAIVEAGKDPRVDGRCVAIIGGSRGAELALLLASHYPDIDA
                     VVAIVPGSAVFPALTDAMTTGGFSLHDKPLPFVPMTWGATPYLLVGDLRGAFETIMQD
                     RAAMQRAAIAVENINGPIQFVSASRDEMWPSKEMADAMMLRLKAKGFRHPVEHLVVQG
                     GHGEPLDEFPKMEAFLDTHFKQACP"
     gene            complement(8167..8370)
                     /locus_tag="PK399_04090"
     CDS             complement(8167..8370)
                     /locus_tag="PK399_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW12335.1"
                     /translation="MSNDIRRLRFARGEMTQQALADACSVTRQTIIALEAGRYAPSLE
                     LGFRIADALGVRIEDVFQWSKPQ"
     gene            complement(8382..9029)
                     /locus_tag="PK399_04095"
     CDS             complement(8382..9029)
                     /locus_tag="PK399_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12336.1"
                     /translation="MAALSARNRLVLWLLAAGVGLALFFTGEYRDLELPLGWIGTVLF
                     VAATWFSVDALYRVPQSEEEAAVAPGEWQAWIGVAFVGAIIGASLLQADVFLPQVPIG
                     QNPATNKAGQSIGTLFVAWLVLAHVLKQRWAGKVLADERDTRIELIASQWGRGATAFC
                     IIGIAVLLGFSDTDRLREFSYPFIAQMLMLTLLWGLWFDQLVAALLYWRDRRAAG"
     gene            9165..10040
                     /gene="trxA"
                     /locus_tag="PK399_04100"
     CDS             9165..10040
                     /gene="trxA"
                     /locus_tag="PK399_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005919399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPW12337.1"
                     /translation="MNDLTKPHVFDATAATFEDTVIRASMDTPVLVDFWATWCGPCKT
                     LGPLLEKLAADYHGAFVLAKVDVDAEQELAAAFQIRSVPMVMLVKGGQLVDGFPGALP
                     EGQLRDFLTHHGIAPAEPVIEDQAPAEAAPPVDPHAEVMRLRRAIDAEPDKAELKLDL
                     ALALLQTGAAAESEKLLDALPANLATDDRTVKARARLGFAALLKDAPPVEVLHAAIAA
                     DPADLRARHFLGVRELVSGNAEAGLQQFIEMLQRDRAFGDGLARKSLIDAFRVVEDED
                     LVGRYRRRMSSLLLV"
     gene            10107..10556
                     /locus_tag="PK399_04105"
     CDS             10107..10556
                     /locus_tag="PK399_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HPW12338.1"
                     /translation="MNASTLRLGMNLWPPFLFSGIRVREISPDWRRVRVELRMRPWNR
                     NVVGTHFGGNLFAMTDPFRMIPVLQCLGRDYLVWDQASSIEFVKPGRGTVHALFELDD
                     ATLDDLRTAAAGGDKVLRWFDTDVIDSQGDVVARVRKQLYVRRKSGR"
     gene            10617..12590
                     /locus_tag="PK399_04110"
     CDS             10617..12590
                     /locus_tag="PK399_04110"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HPW12339.1"
                     /translation="MSGNAYPDVDGYRIHRVIGHGGMSTVYLADQTSLDRKVAMKIML
                     AEALADEVSRARFENEARTIARLQHPNIVGIHEVGRTTDGLPFYSMPFLPRGHLAQRN
                     LRGDQPKVAAIARQLLQALDYAHVRGVVHRDVKAENVLFDEAERPMLADFGIALRRGS
                     NPRLTNAGLAVGSTAYMPPEQARGQEVDRRADLYSMGVLTWEMLVGRLPYNAGDALSM
                     ALKHVQDPIPRLPAPLKHWQDFIDRAMAKRPEDRYASAQDMLQALDQLEARTGKAFVA
                     VDVPTAGEAEPQASPGSRWKPWAVAAAVVAVAVIAAFRLYPVERDVIHAPTTSDTTGP
                     TAGGLPSPQADARTGLLTGMGEGEGTTEGLAAYLANAGQQMRDGNLLSPPNANAWDSM
                     DAAWRVNATHPQTQQLTAQLFDALAEASDRALRAGDVAFSRTAFERARQLDTRRGGEG
                     SAIALLRKRLDAALDTRLQALLGKRDTQGARQLLAETRWLDLDPLRTRALTARIDVAG
                     KAAPTATTGNTVVNATPAAPPPPVATIAVSRADYARFANGTGRAAADCGKGVFGRKRS
                     WNNTGSDRRPVVCVSAADAQAYAAWLGSQDQHRYRLPSAGEVRAQPTTPVSGWLTLCA
                     NRECSQRMASGKARALDAGRGFDDVGIRLVRAD"
     gene            12629..13300
                     /locus_tag="PK399_04115"
     CDS             12629..13300
                     /locus_tag="PK399_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010380413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HPW12340.1"
                     /translation="MSTPPTLGHRLQRLFLTGLLTLLPLWLTWVVVKFLFVLLGDLSK
                     PLIGPSLQGIAARSPQALGWLGDPWVQNAFALVATVLAILLVGWLARRVIGQRLLGWF
                     EAVVARIPLASTIYGSARKLLDILQTKPDGTQRVVLIDFPHAEMKSIGFVTRVIREHG
                     TGRELAAVYVPTTPNPTSGYLEIVPVEKMTPTDWTVDQAMSFIISGGAVAPDTIPFTP
                     PPREA"
     gene            complement(13414..13490)
                     /locus_tag="PK399_04120"
     tRNA            complement(13414..13490)
                     /locus_tag="PK399_04120"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13454..13456),aa:Pro,seq:ggg)
     gene            complement(13525..13881)
                     /locus_tag="PK399_04125"
     CDS             complement(13525..13881)
                     /locus_tag="PK399_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPW12341.1"
                     /translation="MLDPGSNRELPPIPAKRYFTIGEVSELCDVKPHVLRYWETEFPS
                     LEPAKRRGNRRYYQRHDVLMVRQIRSLLYEQGYTIGGARLRLEGEGAKQESALSHQIA
                     KQVRMELEEVLQLLRR"
     gene            complement(13874..14191)
                     /locus_tag="PK399_04130"
     CDS             complement(13874..14191)
                     /locus_tag="PK399_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HPW12342.1"
                     /translation="MALTKAEMAERLYEDVGLNKREAKEFVDAFFDALREALEQGRQI
                     KLSGFGNFDLRRKNQRPGRNPKTGQQIPISARTVVTFRPGQKLKERVEAYSGASHMGA
                     AHA"
     gene            complement(14203..16572)
                     /gene="pheT"
                     /locus_tag="PK399_04135"
     CDS             complement(14203..16572)
                     /gene="pheT"
                     /locus_tag="PK399_04135"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPW12343.1"
                     /translation="MKFSENWLRQHVKTSATRDELAATLTAIGLEVEEMTVLGDALDG
                     VVVARIVECAKHPEADKLQVCQVDAGALGVLQIVCGAPNARPGLVAPLATIGTKIGDL
                     TIKAAKLRGVESNGMLCSAKELGIDADASGLLELPADAPVGAPLADYLGLPDARIELK
                     LTPNRADCFGVHGIAFDVAAATGCDVEPLDTAPVPAAHDAVLPVELNAGADAPRFVGR
                     VLHGVDARAATPVWMAERLRRSGVRPVSFLVDVTQYLMLELGQPMHAYDRDLLQGPIG
                     VRAARDGETLTLLNGSDATVGPQFLVITDADRAVGLAGVMGGEDTKVSSATRNVFLEA
                     AHFAPSAIIGRSRKLGLHTDAGHRFERGVDPELPRIAVERATRLIMDVAGGTPGPVIE
                     AVLPEHLPRPQPIELRRARLARVLGTHVADAEVARILSALGLSVETTTDGWRVTAPAR
                     RFDLAIEEDLVEEIARIHGYDAIPTTLPGGATRLVSPSETRSSEHDARRQLVARDYLE
                     AINYAFVDADLLATWQLADGGVALANPLSAELGVMRTRLLPGLVAALGRNAARQQSRI
                     RLFEIGKTFAASSDAPIETRRIAAVAGGDAAAEQWGEAARKLDFHDIKGDLDSLAALS
                     GATLEYRASTPPFAHPGRSADVYRDGACIGWIGQLHPRLQQALDLDADVYGFELALDA
                     LQARALPRATGLSKYPSVRRDLAFVVPERVSWDAMRATAKHAAGEVLRELRLFDRYAG
                     KGVENGFKSLAMALILQDESRTLIDRDVDAVVADVVAALKSEHDAVIRS"
     gene            complement(16582..17592)
                     /gene="pheS"
                     /locus_tag="PK399_04140"
     CDS             complement(16582..17592)
                     /gene="pheS"
                     /locus_tag="PK399_04140"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPW12344.1"
                     /translation="MSDIESLSTRALADIAAAATPDALEALRVGLLGKSGSITAQLKA
                     LGALPGDQRKAAGEAINRARDAISAALSTRKAALDDAALDARLAGETVDVTLPGRDAA
                     RGGVHPVSRTLERITEIFGRLGYELADGPEIEDDFHNFEALNFPPHHPARAMHDTFYF
                     PDGRLLRTHTSGVQVRYMQDAVSSQSGPPLRMIAAGKVYRSDSDQTHTPMFHQVEGLL
                     VDEHASFADLKGTLAEFVRAFFERDFEMRFRPSYFPFTEPSAEVDIAWQQADGSTRWL
                     EVLGCGMVHPNVLRNVGIDPEKYTGFAFGLGVERFAMLRYGVDDLRSFFDNDVRFLRQ
                     FA"
     gene            complement(17693..18052)
                     /gene="rplT"
                     /locus_tag="PK399_04145"
     CDS             complement(17693..18052)
                     /gene="rplT"
                     /locus_tag="PK399_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPW12345.1"
                     /translation="MARVKRGVTARARHKKILKQAKGYYHARRKVFRVAKQAVTKALQ
                     YAYIGRKQKKRHFRTLWIARINAASRANGLSYSRFMNGLLKAGITLDRKVLADIAVHD
                     AAGFTALTEKAKGALTA"
     gene            complement(18064..18261)
                     /gene="rpmI"
                     /locus_tag="PK399_04150"
     CDS             complement(18064..18261)
                     /gene="rpmI"
                     /locus_tag="PK399_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPW12346.1"
                     /translation="MPKIKTNRAAAKRFRKTASGKYKCGHANKSHILTKKATKRKRNL
                     RQTNHVRAEDAGRLDRMLPYL"
     gene            complement(18544..19083)
                     /gene="infC"
                     /locus_tag="PK399_04155"
     CDS             complement(18544..19083)
                     /gene="infC"
                     /locus_tag="PK399_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPW12347.1"
                     /translation="MGDRNISTTDTKQNRRNQEIRVPRVRVIGSDGEMIGVLSRDEAL
                     RMAEEEELDLVEIQPNADPPVCKIMDFGKFKFELQKKANEAKKKTKQVEIKEVKFRPV
                     TDEGDYQIKLRKMREFLIEGDKIKVNIRFRGREMSHQELGREMAARIEADLGEDIVIE
                     SRPRLEGRQMVMMIAPKKK"
     gene            complement(19156..21060)
                     /gene="thrS"
                     /locus_tag="PK399_04160"
     CDS             complement(19156..21060)
                     /gene="thrS"
                     /locus_tag="PK399_04160"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPW12348.1"
                     /translation="MISITLPDGSQRQFDAPVSVLQVAQSIGAGLAKATVAGEVDGRL
                     VDASDVIDHDAGLRIITPKDQEGVEIIRHSCAHLVGHAVKQLYPDAKMVIGPVIDEGF
                     YYDIHSERPFTPDDLAAIEQRMRELIAQDYDVVKKMTPRDEVIEEFARRGEEYKLRLI
                     DDMSADVTAMGLYFHQEYVDMCRGPHVPNTRFLKAFKLTRISGAYWRGDSKNEQLQRI
                     YGTAWADEKQLKAYIQRIEEAEKRDHRRIGKQQDLFHLQEEAPGLVFWHPKGWSIWQV
                     VEQYMRRVYRDTGYGEVRCPQILDVELWKRSGHWDNYKDNMFFTESEKRTYAVKPMNC
                     PGHVQVFNQGLHSYRDLPIRYGEFGSCHRNEPSGALHGILRVRGFTQDDGHVFCTESQ
                     IESEVRDFHEQALKVYADFGFEDIQIKIALRPESRLGDDATWDKAEDALRSALRASGV
                     EWQELPGEGAFYGPKIEYHLKDAIGRTWQLGTMQVDFMMPGRLGAEYVDETSQKRHPV
                     MLHRAIVGSMERFIGILIEHHAGHFPAWLAPVQAVVMNITDAQADAVADARKSLANQG
                     FRVVSDLRNEKIGYKIREHTLQRVPYLLVVGDREKENGMVSVRTRGGEDLGSMSVDDF
                     AARLRQEQAG"
     gene            complement(21247..21321)
                     /locus_tag="PK399_04165"
     tRNA            complement(21247..21321)
                     /locus_tag="PK399_04165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21287..21289),aa:Val,seq:gac)
     gene            complement(21440..23482)
                     /gene="uvrB"
                     /locus_tag="PK399_04170"
     CDS             complement(21440..23482)
                     /gene="uvrB"
                     /locus_tag="PK399_04170"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPW12349.1"
                     /translation="MTEPSDLDLSTASGGFRLVSPYQPAGDQPQAIEKLVSAFENGIA
                     KQVLLGVTGSGKTYTIANMIQAVQKPTLVMAPNKTLAAQLYGEFKAFFPHNSVEYFVS
                     YYDYYQPEAYVPSSDTYIEKDSSINDHIEQMRLAATKALLSRRDTIVVASVSAIYGLG
                     APADYLSMRLILSRGERIDQRELLRHLTTLQYTRNDIVLDRGTFRVRGETIDVFPAES
                     DLEALRIELFDGEVEQLSMFDPLTGEIFRKIPRFTVYPKTHYATPRERTISAVETIKA
                     ELRERQEQLYAANTLVEVQRLSQRTQFDLEMMAEVGYCNGIENYSRHLTGAAPGEPPP
                     TLFDYLPPDALLVVDESHVTIPQIGAMYKGDRARKETLVDFGFRLPSALDNRPLRFEE
                     WEARAPRSVYVSATPGPYELREAGDEIIELVVRPTGLVDPPVEIRPVATQVDDVLSEI
                     RERVSWGDRVLITTLTKRMAENLTEYLAEHDVKVRYLHSDVDTVERVEIIRDLRLGLF
                     DVLVGINLLREGLDMPEVSLVAILDADKEGFLRSTGSLIQTIGRAARNLRGKAILYAD
                     RITNSMQKALDETDRRRARQVAHNLEHGITPQSVQRAVMDVMEGARYEPGELKGRGKA
                     KRVAEPELDYQRLNPSQQAAKLKALEQQMHQHARDLEFEAAARVRDQIRRLREAIL"
     gene            23640..24167
                     /locus_tag="PK399_04175"
     CDS             23640..24167
                     /locus_tag="PK399_04175"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HPW12350.1"
                     /translation="MRRNAGFTLIELVTTMSVMAILAGIAAPSFAGFIERQRASAAMS
                     SLLTHMSLARVAAITRNRRAVLCPSADGVHCELGTDWSQGWVLFMDEDGNRMPDGSDE
                     IVRVELEPTSRHLRVASTSGRQQLRYLPDGRSAGTNLTISICNKRNELLGQVIVNNMG
                     RPRSERPKVPTACPA"
BASE COUNT         3869 a         8318 c         8039 g         4010 t
ORIGIN      
        1 ccagcctgcc cacggctccc cacgggcgat gcgccggcgc agttcggtcg aggattccgg
       61 gcgcaggccc ggcagcggca agatggcgat gcccccgtgc ggcgacaggg gcaacgcgct
      121 caccggccgc aggcgctccc ccaccaacgc ggcgacctcg ggcgcaatgg catggagcgc
      181 ggccgtctcg atgtcggcgc cgggccgctg caccaccagc aggtgggcgt gttcgcacag
      241 gtcgcgccag cgatgccagg tgtgcaactg gcgcagcgaa tcggcgccca ccagccacgc
      301 cagcggtgcg tccgcgccgt attgactgcg cagctcgcgc agcgtgtcca cgctgtagga
      361 cggcccggcc cgatgcaatt cgcgtcgatc cacgaacagg cccggctcgc cggcgatggc
      421 gagttccagc atctcggcgc gctggtcggc atccgccccc gtcaccgcct tgtgcggtgg
      481 atcgcgtgcg ggcaacagcg cgacctcggc ctgcaatgca tcgcgggcgg tacgggcgat
      541 ggcgacatgg ccgtcatgca ccgggtcgaa ggtgccgccg tagaagatcc gcagcggcgg
      601 tcggttcacg atgccagcag gcgcaccgcg cccggttcgg cgaccgccag cagcaggcgt
      661 tcgagttgct gccaggcatc ggcggattcc tcgcccaggc gcacccgccc cttggaaatc
      721 cggtcgacct tgccggcctc gaccaacatg cgttgccaac gctccggggg atgccgcttc
      781 agtgcgcgcg tgtacaccgc ctgcttggat tcccagacgc gctgcgcctt gaattcggcg
      841 gccaggttgc gcgcacgcgc gagcgcggcg acggtattca gttccttcac caccatgccc
      901 atcagcgccg ggatcgcctc gccctcggcg cggaggccgg ccagcatgcg cgagacctgc
      961 gccgactggc cgttcaacgc ggcatcggtg aggcggaaca cgtcgaagcg ggccgagtcg
     1021 gcgaccagcg actccatctt cgccacgtcc agcaccgcgg gcgcgccggc atcgcgatcc
     1081 agcagcaggg cgagcttgtc gacttcctgc gccgccgcca gcaggttgcc ttcgacccgc
     1141 tgcgccagcc gcgccacggc atccggcgcg gccttgaccc cgcggctgcg caggcgctgg
     1201 ccgatccagc cggtcaattc atgcggcttg atcggccagg cgatgcaggc gtgcccggca
     1261 cgggtaatcg cctcgctcca cttgccccca tgcgagcgcg accagtcgcc cgcgaccacc
     1321 agcacgacca cgtcggaggg cggcgaggcg cacagcccgg cgatcagctt ggcgccctcg
     1381 gtgcccggct tgccggtggg caggcggatc tcgatgaggc ggcggctggc gaacaggctg
     1441 ggggcgttga tcgcggcatc cagcgcgtcc cagtcggcat cacggccttc catgtcgtgg
     1501 acttcgcgct cgtcgatgcc ctgtgcgcgt gcggcggcgc gcacggcgtc ggcggcctcc
     1561 aggaccagca gggtttcgct gccggcgacc agataggcag gacgcagcgg ttcgcccgcg
     1621 agttgcgcgg cgaagcgatc gacgttcagt tccatcgnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnact gcgcaacggc ggccggcttg gaggcggcat cgacccggcg
     1801 caggatcgcc gcggccatgt cgcgctgcag ttcgcgcacc agcaattcgc gttcgctggc
     1861 cttgccgatc gaatcgaccg gcggggccag gtagtcgcgg gccagttcca cggcctgctg
     1921 cggcaccgcc gagctgccgt cggcgcggcg catgctgaag atcaccgcat agcgcagcga
     1981 atattcctgc gcacgaccga actggtcatg gctgatcggg gtatcggccc actgctcgga
     2041 aatcacctgc agggtcgcga cgtcgccgct cgcgtcggcc ggggccgcct gcgcgcctgc
     2101 gcgcttgagc ccgtcggcca gcaactcgcc cagcgggctg tagggatccg aggccagcac
     2161 gcgcaccggc ccgaggttct ccggcaggtt gagggcattg cgcaggtgga agccgcaacc
     2221 ggtgagcagc gcagcggaga gggcgagcaa ggcgaggcgt cgggtcatgg cagcagtcta
     2281 agcgatcggg ttggccaagt cggcaggcat cagcccgcca ccacgatatt cacgatcttc
     2341 cccggcacca cgaccacctt gcggatctcc ttgccggcga cgaaggcggc gacgtgcgga
     2401 tcggccaggg cggcggcctc gaccacttcg cgcgcggcat ctgggacgac ctcgatggtc
     2461 ccgcgcaatt tgccgttgac ctgcaccgcc agggtcaggg catcgcggac cagcgcggca
     2521 gggtccgcgt tcgggaacgg cacgtcttcc agcagggttt ctgcatgccc cagcgcctgc
     2581 cacagcgcat ggctgatgtg cggggtaatc ggattgagca gcaggacgat ggcctcgaac
     2641 gcctcgtggc gaaccgcgcg gcccatgtcg ctcatgtcgt tgaacttgcc gacgtggttc
     2701 agcagctcca tcagcgcggc gatggcggtg ttgaagctgt ggcggcggcc atagtcgtcg
     2761 ccgaccttct ggatcgcctc atggatgtgc cggcgcaggg tcttctgcgc gggatcgagc
     2821 gcacctgcat cgacttcggg atggtcgggc tgggccacgt gcgcatgcac gtcgcgccac
     2881 aggcggcgca ggaagcgcgc catgccctcc acgccggatt cgttccactc cagcgattgc
     2941 tccggcggcg cggcgaacat cgagaacagg cgcaccgtat ccgcgccgta cttgtccacc
     3001 agcacctgcg ggtcgacgcc attgttcttc gacttcgaca tcttctcggt gccgccgatc
     3061 agcaccggca ggccgtcggc ccgcgatatc gcaccggtga cgcgcatgcg ctcgtcgtgc
     3121 cgcagttcga cgtcggccgg gttgatccac tccttcgcgc cgtgctcgtc ctcgcggtag
     3181 aacgtgtcgg cgatgaccat gccctgggtg agcaggcgcg tggccggctc atcgctgccc
     3241 accagcccgg catcgcgcag gagcttgtga tagaagcgga agtacatcag gtgcaggatc
     3301 gcgtgctcga tgccgccgat gtactggtcg accggcagcc agtaattcgc gcgctcgtcg
     3361 accatgtcgg tcgcgcccgg cgaggtgtag cgcgcgtagt accagctcga ctccatgaag
     3421 gtgtcgaagg tgtcggtttc gcgctcggcc gcgccgccgc actgcgggca ggtggtcttg
     3481 cgccattcgg gatcggactt gatcggggaa tgcacgtcgc ccgaggcgaa cgcatcggcc
     3541 acgtcctccg gcaacagcac cggcagttgc tcttccggca ccggcaccga accgcaggcc
     3601 gcgcagtgga tcaccgggat cgggcacccc cagtagcgct ggcggctcac gccccagtcg
     3661 cgcaggcgga aattgacccg gcgcgcgccg gtgccttcgg cctcgaactt cgaggccagc
     3721 gcatcgaacg cggcatcgaa gtccatgccg ttgtaatcgc ccgagttgat cagccagccg
     3781 cgctcggtct gcgcgccttc ctcgtcgatg cggcggaaat actcgcgcac caccgcgacg
     3841 gccgcatcga tctcgaccac gtcgatcgcg gcatgtccgc ccagcgcgac cgccatcgga
     3901 tcgtcgtgct cggcgacatg cgaggacatt tcctgcaggg cgtcgcgcac cgcggcatcc
     3961 acgatcaccg gcttcaccgg caggccgtag gccttggcga attcccagtc gcgctggtcg
     4021 tgcgccggca ccgccatcac tgcgccggtg ccatagccca tcagcacgaa gttggcgacg
     4081 taaaccggga cgtcctcgcc ggtgaccggg tggatcgccc acaggccggt ggccatgccg
     4141 cgcttttcct gggtctccag ttcggcctcg gacacgccgc cctgcctgag gtcggccagg
     4201 aacgcggcca gttccggatt gccggcggcc gccttcagcg ccagcgggtg ctcgcccgcg
     4261 atgctgatga aggtgacgcc catcagcgtg tcggggcggg tggtgaagac ggtcagcggt
     4321 tcggcttcgc catcaatgcg gaactggatc tccaggcctt cgctgcggcc gatccagttg
     4381 cgctgcatcg tcttgaccga atccggccag ccatcgagcg tgtgcaactc atccagcagc
     4441 tgctgcgcgt aatcggtgat gcgcaggaac cactgcggga tctcgcgctt ctcgaccagc
     4501 gcgccggtgc gccagccgcg gccgtcgatc acctgctcgt tggccagcac cgtctggtcg
     4561 accggatccc agttgaccac gctgttcttg cggtaggcca cgcccttctt cagcaggcgc
     4621 acgaacatgc gctgctcgtg catgtagtag tccggcttgc aggtggcgaa ctcgcggctc
     4681 cagtcgatcg cgtagcccag cgacttcagc tgcgcgcgca tgtgctcgat gttggcgtag
     4741 gtccacttcg ccggcgcggt cttgttcttg atcgcggcgt tctcggccgg caggccgaat
     4801 gcgtcccagc ccatcggctg cagcacgttg tgcccggtca tgcgtttgtg ccggctgatc
     4861 acgtcgccga tcgtgtagtt gcgcacgtgg cccacgtgca gcgcacccga cgggtacggc
     4921 agcatcgaca ggcagtagta cttcggcttg tccgactgct cgctgacctc gaaggcgcgg
     4981 gtgctggccc agaacgactg ggcggcggat tcgacggtgc ggggatcgta ggaaacgggt
     5041 tcggacacgg cggaaaactt gcgaaaacgg gcctgacagc ctaccgcagc ccccctccag
     5101 ccgccatgcg tggcaccatc cggccacaac cagggggaaa cgccatgcgc aaacaactgc
     5161 ttgccgccgt cgtgctgacc acgctttgcg tgcccgcgtt cgccgccgat gcgccaggcg
     5221 cgggcaccgc cctgcactgc ggcaagttgt tcgacagccg cagcggcaag ctgctgggac
     5281 cgcacaccgt ggtggtgcgc aacggcaagg tggcggacgt gctggcgggt cgcgtggaag
     5341 ccgtggcgga ggcaaccgcc atcgacctgt ccgaccgcac ctgcatggcc ggctggaccg
     5401 acctgcacgt gcacctgggc agccagtcca gcccgcagag ttactccgaa ggcttccgcc
     5461 tcgacgatgt cgacttcgcc ttccgcagcg tgggctatgc cgagaagacc ctgctcgccg
     5521 gcttcaccag cgtgcgcgac ctcggcggcg acgtcacccc gcacctgcgc gatgcgatca
     5581 accaggggct gctgaaggga ccgcggatct gggccgccgg caagtccatc gccaccaccg
     5641 gcggccatgc ggaccccagc aacggcctca acgaccgatt ggcgcatctg atcggcccgc
     5701 ccggccccac cgagggcgtg atcaattccg tcgacgacgc gcgccaggcc gtgcgccagc
     5761 gctacaagga cggcagcgac gtcatcaaga tcaccgcgac cggcggcgtg ctgagttacg
     5821 ccaaatccgg ggatgcgccg cagttcacgg tcgacgaggt caaggcgatc gtcgacaccg
     5881 ccagggacta cggctaccgg gtcgcggcgc atgcgcatgg cgaggaaggc atgtaccgcg
     5941 cggtcgccgg cggcgtcacc agcatcgagc acggcagcta catgagcgag cgggtgatga
     6001 cgctgatgaa ggaaaaaggc acctggtacg tgcccacgct gtacgcgggc cgcttcgtcg
     6061 ccgacaaggc gaagatcgat ggctacttcc ccgagatcgt gcggcccaag gcggcgcgca
     6121 tcggcgcgca gatccaggac accgcggcca aggcctatcg cagcggggtg aagatcgcct
     6181 tcggaacaga catgggcgtg ggtccgcacg gtgacaacgc gcgcgaattc cagtacatga
     6241 ccgaagccgg catccccgcc gcctacgcgc tgcaggcggc gacgttcctc gcggcagagg
     6301 tgctcggcgt ggacgaccag ggcgtggtcg aagtcggcaa gcgcgccgac atcatcgcca
     6361 tgccgggcga cccgctggcc gacatcggca atacgatgaa agtcgacttc gtcatgaagg
     6421 acggcgtggt ctaccggacg ccacagcgat gaacctggag tggctgggct tcctggccgc
     6481 cgcgtgcacc acgctgtcgt tcgtgccgca ggcggcgaag accatccgca cccgcgacac
     6541 ctccggcatc tcgctgtgga tgtacgtggt gttcaccttc ggcatcgcct gctggttcgg
     6601 ctacggcgtg tacctgatgt cgtggccgat gatcctgtcg aacgcggtca ccttcgtgct
     6661 gtcgtcgacg atcctgggca tgaagctcaa gtacggctga gccgccacgc ggccagcagc
     6721 caccagccga accacagcgc gaacgcgagc cgctgggcga ttcccaccca caatccgatc
     6781 ggcgcaagca cggcgatgat cggcaccatc aggccgatgg cgacgctgcc cagcgcgaag
     6841 ccgatgccgc gccgcgcacc cgccgccagc aacagcgcgc ctggcacgaa cgcgatccac
     6901 cacagcatcc atgccagcac gtgcaggcgg cttgcgtgcg aatcggcatc gcctggatcc
     6961 agcggcagca ggccctgcgc ggcgaaggcc agtgccgaga actgcaccag cacgaggccg
     7021 atccgcgccg cccatccgcc ctcgcgcaag gcgttgcgca gcggcacgcc cgcgactgcc
     7081 agcaacgctc ccggcaccac gaaggccacc aggttgaatg cgaatgccca cggaaggcca
     7141 gccgtgccgc gcagtgccac gggatgcacg cggtgcgaat actcgggcac gccgacgctg
     7201 gccagcagca gcgcgatgac gaagagtcct gcagcgcccc atgcggcaca gccagcgaga
     7261 gacttcatgg gcatgcctgc ttgaagtggg tgtcgaggaa cgcctccatc ttcgggaatt
     7321 cgtccagcgg ctcgccgtgg ccgccctgca ccaccaggtg ctcgacggga tgacggaacc
     7381 ccttcgcctt gagccgcagc atcatcgcgt cggccatctc cttcgacggc cacatctcgt
     7441 cgcgtgatgc ggagacgaac tggatcgggc cattgatgtt ctcgaccgcg atcgcagcgc
     7501 gctgcatcgc cgcccggtcc tgcatgatcg tctcgaacgc gccgcgcaag tcgccgacca
     7561 gcaggtacgg cgttgcaccc caggtcatgg gcacgaacgg caacggcttg tcgtgcaggg
     7621 agaaaccgcc cgtggtcatc gcatcggtca gggccgggaa caccgcgcta cccggcacga
     7681 tggcgaccac ggcatcgata tcgggatagt ggctggccag cagcaacgcc aactccgcac
     7741 cgcgcgagcc gccgatgatc gcgacgcagc ggccatccac gcgcggatcc ttgcccgctt
     7801 ccacgatcgc ggcatgcacg ccatccagcg aaatgcgatc gagcgtttcc ggcgaattcg
     7861 gcgtgccgaa ataccccagc gcgagcaacg catacccctg gtcgaggaag cgctcgcgct
     7921 gcggcgtcca acgcgcgctg gcccaggcat tgccgccctc gccgccgccc agcccgacga
     7981 tcaacggtcg cttgccgccg tcgcgtgcga acagttcgac atcgacttgt ccgtgccgtg
     8041 gcggcagggt ctccgtatcg aagcgttcca ccacccacca catccaggca gccaccagca
     8101 gcgccagcgc caccacgatg ccaagtgtcc atttgacgac ccgcatgccc gtccctgttg
     8161 ccttgctcat tgcggcttgc tccattggaa aacgtcctcg atccgcacgc ccagcgcatc
     8221 ggcgatgcgg aaaccgagct cgagcgaagg cgcgtaacgg cccgcttcga gcgcgatgat
     8281 ggtttgccgg gtgacgctgc aggcatccgc aagcgcctgc tgggtcatct cgccgcgggc
     8341 gaagcgcaag cggcggatgt cgttgctgat ggtggtgccg ctcatcccgc ggcccgccga
     8401 tcccgccagt acagcagcgc cgcgaccagt tggtcgaacc acaatcccca gagcagggtg
     8461 agcatcaaca tctgggcgat gaagggatag ctgaattcgc gcaggcggtc ggtgtcgctg
     8521 aagcccagca gcaccgcgat gccgatgatg cagaacgcgg ttgcgccgcg gccccactgg
     8581 ctggcgatca gttcgatccg ggtgtcgcgc tcgtcggcca gcaccttgcc cgcccagcgt
     8641 tgcttgagca catgcgccag caccagccag gccacgaaca gggtgccgat cgactggccg
     8701 gccttgttgg tggccgggtt ttgcccgatc ggtacctgcg gcaggaacac gtctgcctgc
     8761 aggaggctgg cgccgatgat cgcgccgacg aaggccacgc cgatccaggc ctgccactcg
     8821 cccggcgcga ccgcggcttc ttcctcgctt tgcggcacgc ggtacagcgc atcgacgctg
     8881 aaccaggtcg ccgcgacgaa caagaccgtg ccgatccagc ccagcggcaa ctcgaggtcg
     8941 cggtactcgc cagtgaagaa caacgccagg ccgacaccgg ccgcgagcag ccacaggacc
     9001 agacgattgc gtgccgagag cgctgccatc gatgtcatct ccaccagacc aaatgtatgg
     9061 tgaaatttac atcgacgagt gttgatgtca agtatttttt acatcgggtt gttaacctgc
     9121 gcggcagtcg ccatctcggc gacatcgcac cgaccgggcc cgccatgaac gacctcacca
     9181 agccgcacgt cttcgacgcc accgccgcca ccttcgagga cacggtgatc cgtgcctcca
     9241 tggacacccc ggtgctggtg gatttctggg cgacctggtg cgggccgtgc aagacgctcg
     9301 gcccgctgct cgagaagctg gcagccgact accacggtgc cttcgtgctg gccaaggtgg
     9361 acgtggacgc cgagcaggaa ctggccgccg cgttccagat ccgctcggtg cccatggtga
     9421 tgctggtcaa gggcgggcaa ctggtcgacg gcttccccgg cgccttgccg gaaggccagt
     9481 tgcgcgactt cctgacccac cacggcatcg cgccggcgga acccgtgatc gaggaccagg
     9541 cacccgccga ggccgcgccg ccggtcgatc cgcacgcgga ggtgatgcgg ctgcgcaggg
     9601 ccatcgacgc cgaaccggac aaggccgaac tgaaactcga cctcgccctg gcgctgctgc
     9661 aaaccggtgc cgcggccgaa tcggaaaaat tgctcgacgc gctgccggcc aacctggcca
     9721 ccgacgaccg caccgtgaag gcgcgcgcgc gcctcgggtt cgcggccttg ctcaaggacg
     9781 caccaccggt cgaggtcctg cacgccgcga tcgccgcgga tcctgccgac ctgcgggcgc
     9841 gccatttcct gggcgtgcgt gaactggtgt ccggcaacgc ggaggccggc ctgcagcagt
     9901 tcatcgagat gctgcagcgc gaccgcgcct tcggcgacgg actggcgcgg aagtcgctga
     9961 tcgatgcatt ccgcgtggtc gaggatgagg acctggtcgg tcgctatcgc cgccggatgt
    10021 cgtcgctgct gctggtctga tccggcgagc aagaccatac cgttgcggat ggtaggctgc
    10081 cacacccttc cacgcagccg tcaccgatga acgccagcac cctgcgcctc ggcatgaacc
    10141 tgtggccgcc gttcctcttc agcggcatcc gcgttcgcga gatcagcccg gactggcggc
    10201 gcgtccgggt ggaattgcgg atgcgcccct ggaaccgcaa tgtcgtcggc acccacttcg
    10261 gcggcaacct gttcgcgatg acggacccgt tccggatgat cccggtgctg caatgcctgg
    10321 gccgcgacta cctggtctgg gaccaggcca gcagcatcga attcgtgaag ccagggcgcg
    10381 gcaccgtgca cgccctgttc gagctggacg acgccaccct ggatgacctg cgcacggccg
    10441 ccgcgggcgg cgacaaggtg ctgcgctggt tcgacaccga cgtgatcgac agccagggcg
    10501 acgtggtcgc gcgggtgcgc aagcagctgt acgtgcgtcg caaatccggc cgctgaggtt
    10561 ccatcgacgg ggctgtcctc gtatgctcgg gccgcacccg cccgggaacc ccccctttgt
    10621 ccggcaacgc ataccccgac gtcgacggct atcgcatcca ccgcgtgatc ggccacggtg
    10681 gcatgtccac cgtgtacctg gcggaccaga cctcgctgga ccgcaaggtc gcgatgaaga
    10741 tcatgctggc ggaggcgctg gccgatgaag tcagccgcgc gcgcttcgag aacgaagcac
    10801 gcaccatcgc ccggctgcaa caccccaata tcgtcggcat ccacgaggtc ggtcgcacca
    10861 ccgacggcct gccgttctat tcgatgccct tccttccgcg cggccacctg gcgcaacgca
    10921 acctgcgcgg cgaccagccc aaggtcgcgg cgatcgcgcg ccagttgctg caggcgctcg
    10981 actacgcgca cgtgcgcggc gtggtccacc gcgacgtgaa ggccgagaac gtgctgttcg
    11041 acgaggccga acggccgatg ctggcggatt tcggcattgc cctgcgccgc ggcagcaacc
    11101 cgcgcctgac caatgccggt ctcgcggtcg gcagcaccgc ctacatgccg cccgagcagg
    11161 cacgcggcca ggaggtcgac cgccgcgccg acctttacag catgggcgtg ctgacctggg
    11221 aaatgctggt cgggcgcctg ccgtacaacg ccggcgatgc gctgtcgatg gcgctcaagc
    11281 acgtgcagga tccgatccca cgcctgcccg cgccgttgaa gcactggcag gacttcatcg
    11341 accgggccat ggccaagcga ccggaagatc gctatgcgag cgcccaggac atgctgcagg
    11401 cgctggacca gttggaggcg cgcacgggca aggccttcgt tgccgtggac gtgccgaccg
    11461 ccggcgaggc ggagccgcaa gccagcccgg gcagccgctg gaaaccctgg gccgtggccg
    11521 ccgcggtcgt ggcggtggcc gtcatcgccg cgttccgcct ctatccggtc gaacgcgacg
    11581 tgatccacgc gccaaccacc agcgacacca ccggcccgac agccggcggc ctgccatcgc
    11641 cgcaggccga tgcgcgtacc ggcctgctca ccggcatggg cgaaggcgaa ggcactaccg
    11701 agggcctggc cgcctacctg gccaatgccg gccagcagat gcgcgacggc aacctgctgt
    11761 cgcctcccaa cgcgaatgca tgggacagca tggatgccgc ctggcgggtc aacgccaccc
    11821 atccgcagac ccagcagctg accgcgcaat tgttcgacgc gctcgccgaa gcctccgatc
    11881 gcgcgctgcg cgcgggcgac gtcgcgttct cgcgcaccgc gttcgagcgc gcccgccagc
    11941 tggacacccg ccgcggcggc gaaggcagcg ccatcgcgct gttgcgcaag cgcctggatg
    12001 ccgccctgga tacgcggctg caggccctgc ttggcaagcg cgatacccag ggcgcgcggc
    12061 agttgctggc ggagacccgc tggctcgacc tggatccgct gcgcacccgc gccctgaccg
    12121 cacgcatcga tgtcgccggc aaggcggcgc ccacggcaac gaccggaaac accgtcgtca
    12181 acgccacgcc tgcggcaccg cccccacccg tcgcgaccat cgcggtcagc cgcgccgact
    12241 acgcacgttt cgccaacggc accggtcgcg ccgccgccga ttgcggcaag ggggtgttcg
    12301 gccgcaaacg cagttggaac aacaccggca gcgatcgcag gccggtggtc tgcgtctcgg
    12361 cggcggacgc gcaagcgtac gcggcctggc tgggcagtca ggaccagcat cgctatcgcc
    12421 tgcccagtgc cggcgaggtg cgcgcgcaac cgaccacccc ggtgtccggc tggctgactc
    12481 tgtgcgcgaa ccgcgaatgc agccagcgca tggccagtgg aaaggcgcgc gcgctcgacg
    12541 ccggccgcgg cttcgatgat gtcggcatcc gcctggtgcg cgcggactga aacgccgcgc
    12601 cgggggcatg cgcgcctaga atgcgcgcat gtcgactccc cccacgctcg gccatcgcct
    12661 gcaacgcctg ttcctgaccg gcctgctcac cctgctcccg ctgtggctga cctgggtggt
    12721 ggtgaaattc ctgttcgtcc tcctgggcga tctcagcaag ccgctgatcg ggccaagcct
    12781 gcagggcatc gctgcgcgca gcccgcaggc gctgggctgg ctgggcgatc cctgggtgca
    12841 gaacgcgttc gcgctggtcg caacggtgct ggcgattctg ctggtcggct ggttggcccg
    12901 gcgcgtgatc ggccagcgat tgctcggctg gttcgaagcg gtggtggcgc ggatcccgct
    12961 ggccagcacg atctatggca gcgcgcgcaa actcctggac atcctgcaga ccaagccgga
    13021 cggcacccag cgggttgtgc tgatcgattt tccgcacgcg gagatgaaat cgatcggttt
    13081 tgtcacccgg gtgatccgcg agcatggcac cggccgcgag ctcgccgcgg tgtacgtgcc
    13141 gaccacgccc aacccgacct cgggctacct cgaaatcgtg ccggtcgaga aaatgacccc
    13201 gaccgactgg accgtcgacc aggcgatgag cttcatcatc agcggcggcg cggtcgcgcc
    13261 cgacaccatc ccgttcacgc caccgccgcg cgaagcctga cgacgaatcc atcgcgcgga
    13321 ttcggtagca gacagcgctt cgcaggcaac aaaaaacccg ccatcgtggc gggctcttgc
    13381 tggatgccgg cgatttcaca ccggacttcg aattggtcgg gacggccgga tttgaaccga
    13441 cgaccctctg ccccccaggc agatgcgcta ccaggctgcg ctacgccccg acatctcgat
    13501 cattgtaacc gctgggaccg cggtttagcg cctcaacaac tgcaggactt cttccagctc
    13561 catccgtacc tgcttggcga tctggtggct cagcgcggat tcctgcttcg cgccctcgcc
    13621 ctccaggcga agccgggcac caccaatggt gtagccctgc tcatacagca ggctgcggat
    13681 ctgccgcacc atcagcacgt cgtggcgctg gtagtagcgg cggttgccgc ggcgcttggc
    13741 gggctccagg ctggggaatt cggtttccca gtagcgcaac acgtgcggct tgacgtcgca
    13801 gagttcgctc acttcgccga tggtgaagta gcgcttcgcc gggatcggcg gcagttcgcg
    13861 gttgctgccc gggtcaagca tgcgccgccc ccatgtggct ggcgccgctg tacgcctcga
    13921 cccgctcctt gagcttctgg cccggtcgga aggtgaccac ggtgcgcgcg gagatcggga
    13981 tctgctggcc ggtcttgggg ttgcgacccg ggcgctggtt cttgcggcgc aggtcgaagt
    14041 tgccgaagcc ggacagcttg atctggcgac cctgttccag cgcttcgcgc agcgcgtcga
    14101 agaaggcgtc gacgaattcc ttcgcctcgc gcttgttcag gcccacgtcc tcatacagac
    14161 gctccgccat ttccgccttg gtcaacgcca ttgctgtccc cttcagctcc ggatgaccgc
    14221 gtcgtgttcg gacttcagtg cggcgaccac atcggccacc acggcatcca cgtcgcggtc
    14281 gatcagggtg cgtgattcat cctgcaaaat caaggccatg gcgagactct tgaatccgtt
    14341 ttccacgccc tttccagcgt agcggtcgaa cagccggagc tcgcgcagga cctcgccggc
    14401 ggcatgtttc gcggtggcgc gcatggcatc ccaggacacg cgttccggga cgacgaacgc
    14461 aaggtcccga cggacggacg gatacttcga caagccggtt gcccgcggca acgcacgtgc
    14521 ctgcagggca tcgagcgcca gctcgaagcc gtacacatcg gcatcgaggt ccagcgcctg
    14581 ctgcaggcgc ggatgcagtt gcccgatcca gccgatgcac gcgccgtcac gatagacgtc
    14641 cgcggaacgg cccggatgcg cgaacggcgg cgtcgaggcc cggtattcca gcgtcgcgcc
    14701 cgacaacgcc gccaagctgt cgaggtcgcc cttgatgtcg tggaaatcga gcttgcgggc
    14761 ggcctcgccc cattgctcgg cggcggcatc gccaccggcg accgcggcga tgcggcgcgt
    14821 ctcgatcggc gcatcgctgg acgccgcgaa ggtcttgccg atctcgaaca ggcgaatccg
    14881 cgactgctgg cgggcggcgt tgcgccccag cgcggcgacc agcccgggca gcaggcgcgt
    14941 gcgcatgacg ccaagttcgg cgctcagcgg attggccagg gccacgccgc catcggccag
    15001 ctgccacgtc gccagcaggt cggcatcgac gaaggcgtag ttgatcgcct ccaggtagtc
    15061 gcgcgcgacc agttggcggc gggcgtcatg ctcgctgctg cgggtttcgc tgggcgaaac
    15121 caggcgggtg gcgccgcccg gcaacgtggt cgggatcgcg tcgtagccgt ggatgcgcgc
    15181 gatttcctcg accaggtctt cttcgatggc gagatcgaag cggcgcgcag gcgcggtgac
    15241 acgccaaccg tcggtggtcg tttccaccga aaggccaagc gcggagagga tgcgcgccac
    15301 ctcggcatcg gcgacgtgcg tcccgagcac gcgcgcgagg cgcgcgcggc gcaactcgat
    15361 cggctgcggc cttggcaggt gctcgggcaa cacggcttcg atcacggggc cgggcgtgcc
    15421 acccgcgaca tccatgatca accgggtggc gcgctccacc gcgatgcgcg gcagttcggg
    15481 gtccacgccg cgctcgaagc ggtggccggc atcggtatgc aggccgagct tgcggctgcg
    15541 gccgatgatc gcggacggcg cgaaatgggc ggcctcgagg aagacgttgc gcgtcgcgga
    15601 cgagaccttg gtgtcctcgc cgcccatgac cccggccagg ccgaccgccc ggtctgcatc
    15661 ggtgatcacc aggaactgcg ggccgaccgt cgcatcgctg ccgttgagca gcgtcagcgt
    15721 ctcgccgtcg cgcgcggcgc gcacgccaat cggtccctgc agcaggtcac ggtcgtaggc
    15781 atgcatcggc tggccgagct cgagcatcag gtactgggtg acgtccacca ggaaactgac
    15841 cgggcgcacg ccgctgcggc gcagccgttc ggccatccac acgggagttg ctgcacgtgc
    15901 gtccacgccg tgcagcacgc gcccgacgaa gcgcggcgcg tcggcaccgg cgttcagttc
    15961 aaccggcagg accgcgtcgt gcgctgcggg aaccggcgcg gtgtcgagcg gctcgacgtc
    16021 gcaaccggtc gcggcggcca cgtcgaacgc gatgccgtgc acgccgaagc aatccgcgcg
    16081 gttcggggtc agcttcagct cgatgcgcgc atccggcagg ccgaggtagt cggccagcgg
    16141 cgcgcccacc ggcgcatcgg ctggcagttc tagcaggccg gaggcatcgg catcgatgcc
    16201 cagttccttc gccgagcaca gcatgccgtt cgactcgacg ccacgcagct tggcggcctt
    16261 gatcgtgagg tcgccgattt tcgtgccgat ggtggccaat ggcgccacga gtccaggccg
    16321 tgcattcggc gcgccacaca cgatctgcaa cacgccgagc gcgccagcat cgacctggca
    16381 gacctgcagc ttgtcggctt cgggatgctt cgcgcattcg acgatgcgcg cgacgaccac
    16441 gccgtccagc gcgtcgccga gcaccgtcat ctcttcgacc tcgaggccga tcgcggtcag
    16501 cgtggccgcg agttcgtcgc gcgtcgcgct ggtcttgaca tgctggcgca gccagttttc
    16561 ggagaacttc atcgcggcac ctcaggcgaa ctggcgcagg aagcgcacat cgttgtcgaa
    16621 gaacgaacgc aggtcgtcca cgccgtagcg cagcatcgcg aagcgctcca cgcccaggcc
    16681 gaaggcgaag ccggtgtact tctccggatc gatgccgacg ttgcgcagca cgttcggatg
    16741 gaccatcccg cagcccagca cttccagcca gcgcgtgctg ccatccgcct gctgccaggc
    16801 gatgtcgact tccgcggacg gctcggtgaa cgggaaatag ctcgggcgga agcgcatctc
    16861 gaaatcgcgc tcgaagaagg cgcgcacgaa ttccgccagc gtgcccttga ggtcggcgaa
    16921 gctcgcgtgc tcgtcgacca gcaggccctc gacctggtgg aacatcgggg tgtgggtctg
    16981 gtcgctgtcg ctgcggtaga ccttgcccgc ggcgatcatc cgcagcggcg gcccgctctg
    17041 cgacgacacg gcgtcctgca tgtagcgcac ctgcacgccg gaggtgtgcg tacgcagcag
    17101 gcggccatcc gggaagtaga acgtgtcgtg catcgcgcgc gccggatggt gcggcgggaa
    17161 gttcagcgcc tcgaaattgt ggaaatcgtc ctcgatctct ggaccatcgg cgagctcgta
    17221 accaaggcga ccgaagatct cggtgatccg ctccagcgtg cggctgaccg gatgcacgcc
    17281 gccgcgggcg gcatcgcgac cgggcaaggt gacatcgacg gtttcgccgg ccaggcgcgc
    17341 atccagcgcg gcatcgtcca gcgcggcctt gcgcgtggac agcgccgcgg agatcgcatc
    17401 gcgcgcgcgg ttgatcgcct cgcccgcggc cttgcgctgg tcgccgggca aggcaccgag
    17461 cgccttcagt tgcgcggtga tgctgccgct cttgccgagc aggcccacgc gcaaggcctc
    17521 cagtgcgtcg ggcgtggcgg ccgcggcgat gtcggccagc gcccgggtgg acaacgattc
    17581 gatgtcgctc atctggtttc cgagtcccat aaacacgaat ggggaaggac ttgcgccctt
    17641 ccccacgtga tggcgaccga ggccgccgct acaaccgatg tggagtgacg gctcaggccg
    17701 tcaaagcgcc cttggccttt tcggtcaggg cggtaaagcc cgccgcatcg tgcacggcga
    17761 tgtccgccag caccttgcga tccagggtga tgccggcctt cagcaggccg ttcatgaaac
    17821 ggctgtagct caggccattc gcccgcgagg ccgcgttgat gcgcgcgatc cacagggtgc
    17881 ggaaatgacg cttcttctgc ttgcggccga tgtaggcgta ctgcagggcc ttggtgacgg
    17941 cctgcttggc cacgcggaac accttgcggc gtgcgtggta gtagcccttg gcctgcttca
    18001 ggatcttctt gtggcgcgcg cgcgccgtta caccacgttt gactcgtgcc atgtcttatc
    18061 tcctcacaaa tacggcagca tgcggtccag gcggcccgcg tcctcggcac gaacatggtt
    18121 cgtctgccgc aggttgcgct tccgcttggt cgctttcttg gtgaggatgt ggctcttgtt
    18181 ggcgtggccg cacttgtact tgccggaagc ggtcttccgg aagcgcttgg ccgccgcccg
    18241 attggtcttg atcttgggca ttgggaatcc ttgcgaaatc tgctgactgg tcgaggcggc
    18301 gatttttgta ctttgaggct cgctatcgct cgcctcgaag cccaaaaatc acgctttcca
    18361 tcctgcctga gccggtgata agcctttgat tccgggggaa tcaaggcggg tcctgggtgc
    18421 cactgaacaa caaacccggc gaaccgggcc gcgcattatg gcgggccgat gccgggtttg
    18481 caatgtttgg ctgggaaatc agccagttac ccatctcccg ctggcgggag aaggcaaagc
    18541 gccttacttc ttcttcggcg cgatcatcat caccatctgc cggccttcca ggcgcgggcg
    18601 cgattcgatg acgatgtcct cgccgaggtc ggcctcgatc cgcgcggcca tctcgcggcc
    18661 cagttcctgg tggctcatct cgcggccacg gaagcggatg ttgaccttga tcttgtcgcc
    18721 ttcgatcagg aattcgcgca tcttgcgcag cttgatctgg tagtcgccct cgtccgtgac
    18781 cggacggaac ttcacttcct tgatctcgac ctgcttggtc ttcttcttgg cctcgttggc
    18841 cttcttctgc agttcgaact tgaacttgcc gaagtccatg atcttgcaga ccggcggatc
    18901 cgcgttgggc tggatctcga cgaggtcgag ctcctcttcc tcggccatgc gcagggcttc
    18961 gtcgcgcgaa agcacgccga tcatctcgcc gtcgctgccg atgacccgga cgcgcgggac
    19021 gcggatttcc tggttcctgc ggttctgctt cgtgtcggtg gtactgatgt tgcgatctcc
    19081 aatggagtgg catcgttgga tgccggatcg gccacgattc cggaatcgtg gccggctgtg
    19141 gacaggcgac tggggtcagc cggcctgttc ctggcgcaaa cgcgcggcga agtcgtcgac
    19201 cgacatgctg ccgaggtctt ccccaccccg cgtccgcacc gaaaccattc cgttttcctt
    19261 ttcgcggtcc ccgaccacga gcaggtacgg cacgcgctgc agcgtgtgct cgcgaatctt
    19321 atagccgatc ttttcgttgc gcaaatcgga aaccacccgg aagccttgat tcgcaaggct
    19381 tttccgggcg tcggcgacgg catcggcctg ggcgtcggtg atgttcatga ccaccgcctg
    19441 caccggcgcc agccaggccg ggaagtggcc ggcgtggtgc tcgatcagga tgccgatgaa
    19501 acgctccatc gatcccacga tggcccggtg cagcatgacc ggatggcgct tctggctggt
    19561 ttcgtcgaca tattcggcgc ccaggcggcc cggcatcatg aaatcgacct gcatggtgcc
    19621 gagctgccag gtccggccga tcgcatcctt caggtggtac tcgatcttcg ggccgtagaa
    19681 cgcgccctcg cccggcagtt cctgccattc caccccggag gcgcgcagcg cgctgcgcag
    19741 cgcgtcctcg gccttgtccc aggtggcatc atcaccgagg cgggattcgg gccgcagcgc
    19801 gatcttgatc tggatgtcct cgaagccgaa gtcggcatag accttcagcg cctgctcgtg
    19861 gaagtcgcgg acctcggact cgatctgcga ctcggtgcag aacacgtggc catcgtcctg
    19921 ggtgaacccg cgcacgcgca ggatcccgtg caacgcaccc gacggctcat tgcggtggca
    19981 gctgccgaac tcgccgtagc ggatcggcag gtcgcggtag ctgtgcaggc cctggttgaa
    20041 cacctgcacg tggccggggc aattcatcgg cttcaccgcg taggtccgct tctcggactc
    20101 ggtgaagaac atgttgtcct tgtagttgtc ccagtggccg gagcgcttcc acagctcgac
    20161 gtcgaggatc tgcgggcaac gcacctcgcc gtagccggta tcacgataga cgcggcgcat
    20221 gtactgctcg acgacctgcc agatgctcca gcccttcggg tgccagaaca ccaggcccgg
    20281 cgcctcttcc tgcaggtgga acaggtcctg ctgcttgccg atccgccgat gatcgcgctt
    20341 ctctgcttcc tcgatgcgct ggatgtaggc cttcagctgc ttctcgtccg cccaggcggt
    20401 gccgtagatg cgctgcagct gctcgttctt ggaatcgccg cgccagtacg cgccggagat
    20461 gcgggtgagc ttgaacgcct tgaggaagcg cgtgttcggc acgtgcggtc cacggcacat
    20521 gtccacgtat tcctggtgga agtacaggcc catcgcggtc acgtcggcgc tcatgtcgtc
    20581 gatcaggcgc agcttgtatt cctcgccgcg gcgcgcgaat tcctcgatga cctcgtcgcg
    20641 cggggtcatc ttcttcacca cgtcgtagtc ctgcgcgatc agttcgcgca tgcgctgctc
    20701 gatcgcggcc aggtcgtccg gggtgaacgg gcgctcggag tggatgtcgt agtagaaacc
    20761 ctcgtcgatg accgggccga tcaccatctt ggcgtccggg tacagctgct tcaccgcatg
    20821 gccgaccagg tgcgcgcagc tgtggcggat gatctccacg ccctcctggt ccttcggggt
    20881 gatgatgcgc aggccggcgt catggtcgat gacgtcgctg gcatcgacca ggcgtccgtc
    20941 gacttcgccg gccacggtgg ccttggccag gccggcgccg atcgactgcg cgacctgcaa
    21001 cacggaaacg ggggcatcga actggcgctg ggagccgtcg ggaagggtga tcgatatcat
    21061 gggcatgggg tccggaaaac gaaaaaggcg ccgaggcgcc tttcggttgc tcaccacgag
    21121 ggagcgcgat tgcgtcggca ggattcagcg gattaggcga tcgtagtgct catgtcgcac
    21181 gctcggccgg cgttgccgcg ggccacctgc tctctggtgt cgatgcgttg ccacgctgct
    21241 ggagcgtggt gggcggtaca gggttcgaac ctgtgacccc taccatgtca aggtagtgct
    21301 ctaccgctga gctaaccgcc cggtatcccc aggaccacgg gggcgcgaat tgtagcccgc
    21361 ggcaggcagg tggacaagca gccggcggaa ctttgattcc ccgtcatccc ggccaacgcc
    21421 gggatgacgg caagaacccc tacaggatcg cctcgcgcag cctgcggatc tggtcgcgca
    21481 cgcgggccgc ggcctcgaat tccaggtcgc gggcgtgctg gtgcatctgc tgctccagcg
    21541 ccttgagctt ggccgcctgc tgggacgggt tgagccgctg gtagtccagt tcgggctcgg
    21601 cgacgcgctt ggccttgccc cggcccttga gctcgccggg ttcgtagcgc gcgccttcca
    21661 tcacgtccat caccgcgcgc tgcacggatt gcggcgtaat cccgtgttcc aggttgtgcg
    21721 ccacctgcct ggcgcgccgg cggtcggttt cgtccagtgc cttctgcatc gagttggtga
    21781 tgcggtccgc gtacaggatc gccttgccgc gcaggttgcg tgcggcgcgg ccgatggtct
    21841 ggatcagcga gccggtgctg cgcaggaagc cctccttgtc ggcatcgagg atcgcgacca
    21901 gcgagacctc gggcatgtcc aggccttcgc gcagcaggtt gatgccgacc agcacgtcga
    21961 acaggcccag gcgcaggtcg cggatgatct cgacgcgctc caccgtgtcc acgtccgagt
    22021 gcaggtagcg caccttgacg tcatgttcgg cgaggtattc ggtgaggttc tccgccatcc
    22081 gcttggtcag cgtggtgatc agcacgcggt cgccccagga gacgcgctcg cggatctcgg
    22141 agagcacgtc gtcgacctgg gtcgccaccg ggcggatctc caccggcgga tcgaccagcc
    22201 cggtcgggcg caccaccagc tcgatgattt cgtcgccggc ctcgcgcagc tcatacggcc
    22261 ccggcgtcgc cgacacatac acgctgcgcg gtgcgcgggc ctcccattcc tcgaaacgca
    22321 acggccggtt gtcgagcgcc gacggcagcc ggaacccgaa atcgaccagc gtttccttgc
    22381 gcgcgcggtc gcccttgtac atcgcgccga tctgcggaat cgtcacgtgc gattcgtcca
    22441 ccaccagcaa tgcatcgggc ggcaggtaat cgaacagcgt cggcggcggc tcgcctggcg
    22501 cggcgccggt gaggtggcgc gaatagttct cgatgccgtt gcagtagccg acctcggcca
    22561 tcatctccag gtcgaactgg gtgcgctggc tgagccgctg cacttccacc agcgtgttcg
    22621 ccgcgtacag ctgctcctgg cgttcgcgca attcggcctt gatcgtttcc accgcggaga
    22681 tcgtgcgctc gcgcggcgtg gcgtagtgcg tcttcggata cacggtgaag cgcgggatct
    22741 tgcggaagat ctcgccggtc agcggatcga acatcgagag ctgctcgacc tcgccgtcga
    22801 acagttcgat gcgcagcgct tccaggtcgg attcggccgg gaatacgtcg atggtttcgc
    22861 cgcgcacgcg gaaggtgccg cgatcgagca cgatgtcgtt gcgggtgtac tgcagcgtgg
    22921 tcaggtggcg cagcagttca cgctggtcga tgcgctcgcc gcgcgacagg atcagccgca
    22981 tcgacaggta atccgccggc gcaccgaggc cgtagatcgc cgagacagat gccaccacga
    23041 tggtgtcccg gcgcgacagc agggccttgg tcgcggccag gcgcatctgc tcgatgtggt
    23101 cgttgatcga gctgtccttc tcgatgtagg tgtcgctgga cgggacgtag gcctcgggct
    23161 ggtagtagtc gtagtagctg acgaaatact ccaccgagtt gtgcgggaag aacgccttga
    23221 actcgccgta aagctgcgcg gccagggtct tgttgggtgc catcaccagg gtcggtttct
    23281 gcaccgcctg gatcatgttg gcgatggtgt aggtcttgcc ggagccggtg acgcccagca
    23341 acacctgctt ggcgatgccg ttctcgaatg cggagacaag cttctcgatg gcctgcggct
    23401 ggtcgcccgc gggctggtac ggcgagacca gccggaagcc gcccgaggcg gtggacaggt
    23461 cgaggtcgga aggctcggtc atgggggagt tcgcgaaggc caaccgggga gtttacgccg
    23521 cccgcgtgca cgacttgaga cgcaggttca gggttctcct accggggctg gcaccttcgc
    23581 ccgattatcc ggcgctggcc tccgctctag cctgcttcca acccatcggg agccctgcca
    23641 tgcgccgcaa tgccggtttc accctgatcg aactggtgac cacgatgtcc gtgatggcca
    23701 tccttgccgg gatcgccgcg ccgtcgtttg ctggcttcat cgaacgccag cgcgcctccg
    23761 ccgcgatgtc ctctttgctg acccacatgt cgctggcgcg ggtcgccgca atcacccgca
    23821 accggcgcgc cgtgctctgc cccagcgccg acggcgtgca ctgcgagctc ggcaccgact
    23881 ggagccaagg ctgggtgctg ttcatggacg aagacggcaa ccgcatgccc gatggcagcg
    23941 acgaaatcgt ccgcgtcgag ctggaaccca ccagccggca cctccgagtc gccagtacca
    24001 gcggtcggca acaactgcgc tacctccccg acggacgcag cgctggcacc aacctgacga
    24061 tctcgatctg caacaagcgc aatgaactgt tgggccaggt gatcgtgaac aacatgggac
    24121 gcccacgcag cgagcgcccc aaagtgccga ccgcctgtcc cgcctaagcg tcatccgaac
    24181 agcgcagcac ttcgtcggcg ttccgcggcg cgaaggcagc ctgcgtcacc caccgaacct
    24241 gcggcttgcc cgcttcggtg cggccgacta caatgcgctc cccgcccgaa tagctcagcc
    24301 ggttagagca cttgactgtt aatcaggggg tcgttg
//
LOCUS       DAOOCX010000027        24224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1757_length_24224_cov_8.485001, whole genome shotgun sequence.
ACCESSION   DAOOCX010000027 DAOOCX010000000
VERSION     DAOOCX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 24224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24224
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1757_length_24224_cov_8.485001"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..2209)
                     /locus_tag="PK399_04180"
     CDS             complement(113..2209)
                     /locus_tag="PK399_04180"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HPW12351.1"
                     /translation="MNTPKTLLLSLSIAAALAGCQKPAEAPAASTAAAETKTPAYTLD
                     ESKLPPVNRFLIGDLDTSKNACTDFGGYVNGKWLAANAIPADRTSWGAFEMLDERSTA
                     AQRQLAEQAAAMKDATGVEKIVGDLYATGMDAAKINAQGITPIKGHLDAIAALADKEA
                     IAEYLRTSAAKGENNLFGFGPNADFNDSTVNIAYATQGGLGLPDKGYYEDADKADKLA
                     AYQAHVAKVLELSGIAAADAVKQAADVVAFEKRLAKVSKSSEQMSRDVKIFYNPVSPA
                     DADKLAPNFPWTKFFESQGVATPKMFSLAMPEFHAEVSKMLADVPAAQWQSYLRFHTV
                     DGASPYLSDAFVQENYNFYNKTLRGQKELKERGKRVLDVIENQAGEALGQMYVKVAFT
                     PESKARMEALVKNLSEALKARIENLAWMSPETKKKAMEKWASFTPKIGYPDKWRDWAG
                     LATSRDSYIGNVLAANEFNYKWNLGKIGKPVDKTEWGMSPQTVNAYYNPQQNEIVFPA
                     AILQPPFFDPNATDEVNYGGIGAVIGHEMTHGYDDQGSRFGPTGNFEDWWTPADAKGF
                     SGLTDKLVAQFNGYEAAKGAMVNGKLTLGENIADLGGLATAYDAMKKATAGTPDPKTD
                     GMTRDQRFFANWATVWRRNFSPEELKVRLTTDSHAPANFRAIGAPSNMPAFAQAFGCK
                     VGDPMVRDGDKQVVIW"
     gene            complement(2298..3707)
                     /locus_tag="PK399_04185"
     CDS             complement(2298..3707)
                     /locus_tag="PK399_04185"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPW12352.1"
                     /translation="MSFFRNVSIALIAAFAFNGAIAAAASEPSVKLPDYQTITLDNGA
                     TVLLMPRKDVPLIAANIAVRGGALADAPGKEGTADILGEMLSKGAGSRSALQFAQTVD
                     GAGGSIGFGSSREAITANAQFLSKDSALMLSLLADALLRPTMDSAEFDKLRKRAIDGI
                     ANAKDSDPRQLIGLYSGGWLFRGHPYGRSTGGDETSLATITLADLQAFRQQQMGGDRL
                     VIAIAGDFDAKAVTAQLKQSFGGWAKATGTLPQVEAKARETGRRVLLVDKPGATQTYF
                     ALANVGSKRGDPAEAAQDLVQTAFGGRFTSMLNTELRVKSGLTYGARSGIERASQPGP
                     AVISSFTKTETTKAAIDLAIATLDRLHRDGLDAATIESAKRYVAGQYAPGLETAPQIA
                     AQLVDMTLYGESREAVDGYLGKIAAATPAQIAEARMVFPDSKDLAIVAIGDASKIRDW
                     MKAYGPLTEMKLTDPRFSP"
     gene            complement(3704..5086)
                     /locus_tag="PK399_04190"
     CDS             complement(3704..5086)
                     /locus_tag="PK399_04190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPW12353.1"
                     /translation="MRLLSLACAIALGLSSVAAQAATPQPAANVPGSEAIVSKTLANG
                     LKIVVWPDHDIPNANYYTFYKAGSRNEYPGITGLAHYFEHMMFNGTTKRAPGEFDKQM
                     EAAGGSNNASTSNDLTIYTDWFPSSALETIFDLEGDRMANLSFDPKVIESERGVVFSE
                     RRSSIDNDNFGTLMEQMQATAWTAHPYQFPTIGWPSDIEGWKIDDLKKFFKTYYAPNN
                     AVLVIAGDVDPAQVFALADKYLAPLPKQPAPAPITTVEPEQQGERRLVVERADAQSPI
                     VAYAFHSGLGAGSKEFPTLELLTTILSQGESSRLHQRLVEKEQAATQAGAFAEAGFDP
                     GLLWIYAMLPPGGDMAKVETLLDDELAKIARDGVSAAELEKARNLKSSAFWRGMATIN
                     GKARALGTYEVFNGDYRKLFDAPAAYDAVSAADVQALAKKILRIQNRTTGLLKPVAAA
                     PTAADKEAAK"
     gene            complement(5197..5619)
                     /locus_tag="PK399_04195"
     CDS             complement(5197..5619)
                     /locus_tag="PK399_04195"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPW12354.1"
                     /translation="MNHARPAGARPDCALLGFRLLVALLIGIHGWHRLHAGTSPIFGD
                     WLATQDIPLAHAIAWGVTISEIIGSACLAVGLCVRPFALLFMAIYAMGIVLVHAPEGW
                     FVVGAGRNGMEYSVLLIGSLALVALRIRTPLWHAFGSR"
     gene            complement(5616..6134)
                     /locus_tag="PK399_04200"
     CDS             complement(5616..6134)
                     /locus_tag="PK399_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPW12355.1"
                     /translation="MGATTRPLGTRDKPESSRVFFLRASTGLATGYPWWPLADALRLL
                     RIVTALLFMAHASMRFVLGTILQFGVFMESQGFPHGQAWVLAITAYELGAGSLLLFNR
                     GVRWAASGLAVIVATGIWLIHRHNGWFVGEHGTGGSEYSVALLAMLLVIAADDATRTS
                     RPIPSSREADPT"
     gene            complement(6139..9213)
                     /locus_tag="PK399_04205"
     CDS             complement(6139..9213)
                     /locus_tag="PK399_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW12356.1"
                     /translation="MPAARHANHCKHTTLCLALAIGLGIAGLASAQSNAAGAIHGRAS
                     QGDVVVIENAATGYRREITVGADGGYRAPQTPSGNYTVTVRRADGSTRVRERVAVSVG
                     TGTQVDFSADDTTTLEAVTVRAGSINPVDVSSVESTTILSAQQMASIPVARDATSVAL
                     LAPGTVRGDAAFGNLASFGGSSVAENAYYVNGFNITNAFNNLAFAQIPFEAIAEQQVK
                     TGGYGAEFGRSTGGVVNLITRRGTNDFSAGGALYWTPSSLSGKTPDIFLNNGDLLQDN
                     SRDEAEQSSVALWAGGALVRDRLFAYGLVQFARNHGDAWNDVNAGINANTRTRSPTWL
                     LKMDWNLAQDHLLELTSFSDVARTTQVVHTNDEGVLNRTGVLGTVYTEQGGTSHALKY
                     TGYLSDSFTLSALFGRGKFSRSIYGISANGTRQEWSGDINAPDTGCPVIVDQRNSTLL
                     GATPSIRGCDFIGGTLGRPDAGDTRRQFRVDVDWQLGDHQLRFGYDADRFTSVAGGAI
                     EGGQVWNYATIDPDGVPLNDDDLDFVVNVVFKNGATVDVDQRAFYIEDRWQVTPNFLA
                     YAGLRWDSFDNRNGNGESYASIDNQLGPRLGFAWDVKGDASLKVFGNAGRYALPLTAT
                     VAIRGASASIFSQQLFFYDSVDPATGAPVGATPFTPITYLNNEFGAQKDPRTIASENL
                     QPMYQDEFILGFQKQVDDHWSFGVRTVFRDLRRAIDDNCDERAIYDWAIANGFVDPDG
                     DPFQSPADSTVPKEIAFINPGFTFCHLYNPGSDGVFNIDIDGNGTLEKVTIDADTLGP
                     KAKRTYAAAEFFFEGHWDKLFLQGSYTWARSTGNTEGGIKSDIGQADTGTTQDFDYRE
                     LMLGADGPLPNDRKHTLKVFGNYELNDQWSIGGNLLVQSGRPINCFGVYGNDPLHYGA
                     SYFSCSDAPGTDADGDGVLDNGTALVPRGSAGRTPWQRQLDLNVAWKPAFAAGHLTLK
                     LDVFNVFNSRTVTSVVETAETGQGSSLYDTVYKIPTGFQAPRAVRVMLQYAF"
     gene            9375..10535
                     /locus_tag="PK399_04210"
     CDS             9375..10535
                     /locus_tag="PK399_04210"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPW12357.1"
                     /translation="MDRAEDTRWLPLPPRPWWTLWLSAFVAWTAYGLLYCYQVMSMAE
                     DMGKHYAWSDVLRINLIGMWGWVPLTVALFWLVSRVPIRRGSVARSCTILMTAAFAVV
                     VLRSVYIYTLDPWVGWYDTPPDFWSDVMPTSLNNNVLISWIVIGLAHALLFSDQARAQ
                     ERRAAELQGLLAQARLDAIAAQLNPHFLFNALNSIAELVHHDADRADRMLVGLSALLR
                     RSLDSSRLQWVSLREELELLAQYLEIEKVRLGARMQVQWNVAEECLDEAVPPQLLQPL
                     VENAVVHAIARRIRPGSVRIGAGLVGAALWLDVEDDGADGLPDVEGHGIGLANTRARL
                     QVLYGDEAVLSIEATANGGTRVGLRLPRRHAIPASTRDRPARDLRFDEVRVP"
     gene            10532..11290
                     /locus_tag="PK399_04215"
     CDS             10532..11290
                     /locus_tag="PK399_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPW12358.1"
                     /translation="MSQLRVMIADDEPLARLRLRRLLGGIAGLEVVAECASGIEVMQL
                     LPDCPCDLLFLDIQMPGADGFQALEHVVARPRAVVFVTAYAEHAVRAFDVQAVDYLLK
                     PISAERLLQAVERAQRALANDAPLAAPPAIPRRLALPIGRRMQMVELDELDCALAQAN
                     YVELKVGTRSFVLRDTISGFHARLDPQRFLRIHRSAIVRIDAIREVEPLASGRYRVNL
                     HGGQRLVSGRSYRDALRAALGLAPEASLPSRISA"
     gene            11350..12723
                     /locus_tag="PK399_04220"
     CDS             11350..12723
                     /locus_tag="PK399_04220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW12359.1"
                     /translation="MRVHFHGAAGEVTGSMHLVEAAGKRVLLDCGMRQGSAEMEAGNV
                     DPFPFDVASLDALVLSHAHIDHIGRVPLLVKRGFRGPIFAQQATADLMPIMLLDSASL
                     AESDAARFNRRRTHGEVEQQPLYTKDEVSDAMALVRALPYDARQPIVPGIEIAFRDAG
                     HILGSSIVELWADGRKLVFSGDLGPTGTPILRDPTPIRDADLVLMESTYGDRDHKDRA
                     ETIVELGGILEQAWRDGGNVLIPAFAVGRSQELLYWFAKYWDEWDMSRWQVFLDSPMA
                     GKVVQAYGRHHDLFDDEARRVWRDAPNPFKLPNLHITASTEESMAINRVQRGAIVIAG
                     SGMANGGRILHHLRNNLGRREAHVVFVGYQGAGTIGRRLVDGARWIRIHGRDIRVNAT
                     MHTVGGLSAHADQRGLLAWFGHFDPKPALALVHGEDKAREALAGEIGERHGIEVVLAR
                     PGMTLDA"
     gene            12723..13460
                     /locus_tag="PK399_04225"
     CDS             12723..13460
                     /locus_tag="PK399_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPW12360.1"
                     /translation="MFGFGRSATFLLLALLAEIAGTVGGFGSSVFFVPVANMYFDFQS
                     VLGITALFHLASNISKLGLFRGGIDRGLLLRLGVPSVAFAALGGLATTWFPTRWLEAV
                     LAVFLLGLSGWMLLRPRWRLAPTTRSAVVGGALSGGMAGLVGTGGAVRGMTLAAFDLR
                     KEVFIATSAAIDMAIDLSRAAVYARNGYIHAHDLAWVPLLAVVAITGTWVGKRILARM
                     PQVLFRRIALLLVFAIGLVSLFQAVRA"
     gene            13482..13901
                     /locus_tag="PK399_04230"
     CDS             13482..13901
                     /locus_tag="PK399_04230"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPW12361.1"
                     /translation="MSELKTRPTAASVAEFLAQQGDQRRADCEAVLRMMEAVTGERAE
                     MWGAAIVGFGRYAYRGNSKKAMEWPIIGFSPRKNDLTLYLMPGFEGRAELMARLGKHK
                     TGKSCLYLKKLADVDARVLRELIDCSVRGMDPQRVRR"
     gene            complement(13911..15083)
                     /locus_tag="PK399_04235"
     CDS             complement(13911..15083)
                     /locus_tag="PK399_04235"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HPW12362.1"
                     /translation="MRANRQPTLRSAGGHERSQVGMVELFFDLVFVFAITQLSHGLLE
                     QLTPLGALHTGVLFIGAWWLWIYTTWCTNWLNPAATPVRQLLFVLMLLALVMAAALPH
                     AFAERGLVFALAYVAQHLLRSAYMVHAFGAVSGHGRNFIRIFAWLAVSGTCWVLGGLS
                     DPQSRLAWWTAAIAIEFIGPFAYYRVPGMGASTTTDWDVDPHHMAERCGLFVIIALGE
                     SLLITGATFAGLAWETPQLLAFIAAFVGTVAMWWLCFDTGSGRAIHHFEHAADRGRVA
                     RLAYTYLHIPIVAGIILCAVADELVLVHPDHASNAGIATILGGPFLYLLGNGLFKWAT
                     NQRRLPPISHLVGMGLLLALSPFAFGHAFSALALGGLTSGTLVLVALWEWRALQQA"
     gene            complement(15086..15433)
                     /locus_tag="PK399_04240"
     CDS             complement(15086..15433)
                     /locus_tag="PK399_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12363.1"
                     /translation="MKPEIRIASWLMASVFLVMGGYRLWQALQGVPTANATLAFSAGE
                     FLLGIAIAAGWRLRAMALLGALLMLVDAVLSHRFWTLQGAEQGAQLLHFMKNIGFVGG
                     LFLLSTVVTGKRR"
     gene            15757..17088
                     /locus_tag="PK399_04245"
     CDS             15757..17088
                     /locus_tag="PK399_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW12364.1"
                     /translation="MTFESLGLAPALLRALADNDYNTPTPIQAQAIPLVLSGRDVLGG
                     AQTGTGKTAAFALPVLNRLSKETPPAGPRKPRALVLVPTRELAVQVADSFKTYGRHLK
                     LNVTTLFGGVGMQPQIEQLRRGVDVLVACPGRLIDHLDRGSVKLDAVEVLVLDEADRM
                     LDMGFLPAIKRVLNRLPKQRQTLLFSATFEAQIKALALEFMTNPEQVQVAAQNTIAQT
                     ITHTAHPVDGSRKRDLLIQILSQRHTDQVLVFGKTKHGCNRLAEQLEEAGLPSVAIHG
                     NKSQAARQKALKDFKSGKARILVATDVAARGLDIPDLPLVINHDLPMVAEDYVHRIGR
                     TGRNGAVGQALSLVSPEEGGLLRQIQRLLKVDLVMDVVPGFAPSKPIRLDTPIPKNGG
                     NGGQRPPGRPGAAPRPHARPAARAGHAGQQHRPSGQGAYAGKRNAPRGTRA"
     gene            complement(17355..18149)
                     /locus_tag="PK399_04250"
     CDS             complement(17355..18149)
                     /locus_tag="PK399_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HPW12365.1"
                     /translation="MDTTASVSPLRLLDDRATRLFVALAAFFCANAVLAEFIGVKIFA
                     LEDSLGIAPLEWNLFGQSGSLNFTAGTLLWPIVFIMTDTVNEYFGKRGVRMISWLAAG
                     LIVYSFVFAYLAIHLAPASWWVGAAKDQGVPDYQAAYAAIFGQGMWIIVGSLIAFLVG
                     QLIDVQVFHRIRSRTGERHAWLRATGSTAVSQLVDSFVVLYIAFVLGPQQWPTSLFLA
                     VGTLNYVYKMLAAIALLPLLYLLRAGIHRYLGRARADELREHAAEG"
     gene            complement(18206..19201)
                     /locus_tag="PK399_04255"
     CDS             complement(18206..19201)
                     /locus_tag="PK399_04255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HPW12366.1"
                     /translation="MKAVVCDAWGTPDTLQVKDLQLPEPAPGQVRVRVRAAAVNFPDA
                     LLVAGKYQFKPDFPFSPGAEFSGTVSALGDGVSSFAIGDQVVGTTAFGAYAEEVLADA
                     AQVIGLPNDTSDDELQLAGSFLLTYGTSYHALKDRAAAKPGESLLVLGAGGGVGLAAV
                     ELGKRMGLRVIAAASTEAKREAARSRGADEAIDYTTDDFRGQLKTLTNGRGVDIVYDP
                     VGGDIAETALRSVGWGGRYLVVGFAGGGIPKLPTNLLLLKGSALVGVFWGEFVRREPE
                     AHAANTRQLLAWLHDGTLRPLVSARYPLSGAAQALDALLGRDAIGKLVIVPSLAP"
     gene            complement(19221..20054)
                     /locus_tag="PK399_04260"
     CDS             complement(19221..20054)
                     /locus_tag="PK399_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPW12367.1"
                     /translation="MGNPKRDLQSLAADAEGRNPQLVNSVARAFSILRCFEQVRSHPE
                     HRYLGNQEIARRTRLPKATVSRLSQTLATLGYLEYSPTLEKYALGPAVLGLGHAYMAG
                     HDVIGIAQPLMQELADYTQAAVMLAVPEGMRMLLLEVCQGDAMFNMRLEKGARVPHGT
                     TALGRADLASRSRDAFDGYLAELERTSSPAGWAQIRAGIERARQDYETYGFVFSLGDW
                     NPDVFAVGVPMISADRTRRFAFNISGRVSVMTREKLVQDFGPRLVALRNRVYEATEGR
                     F"
     gene            complement(20054..21253)
                     /locus_tag="PK399_04265"
     CDS             complement(20054..21253)
                     /locus_tag="PK399_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPW12368.1"
                     /translation="MHRRLHAFLQERVWPMEAAHAAEDAANRAAGNPWQPSEVIEGLK
                     AEARAQGLWNLFLPNSTHAPEGLSNLDYAPLCELMGRMYWSPEVFNCSAPDTGNMEVL
                     ARYAAEPLQARWLQPLLDGRIRSAFLMTEPAVASSDATNLQCTIRRDGDDYVINGRKW
                     YASGAGDPRCAVYIVMGITDANAAPHQRHSMIVVPADTPGIRVNRAMDVYGYDDAPHG
                     HMEIDLVDVRVPAANMLLGEGRGFEIAQGRLGPGRIHHCMRAIGMGERALEAMCRRLA
                     SRHAFGRAIADQSIWRERIAESRCLLDQARLMVLNAARMMDTTGNKAARAEIAMIKVI
                     APQAAEKVIDWAVQAHGAMGVSQDSFLAKAWAHNRTLRLADGPDEVHRDTVAKLELKK
                     YATKDAA"
     gene            21248..22213
                     /locus_tag="PK399_04270"
     CDS             21248..22213
                     /locus_tag="PK399_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPW12369.1"
                     /translation="MQFNGAGTGREIHVFHYAEHWFRWRWDRMAHSVWVQVNDILRCV
                     AKSALPSACGVGADGLDYSKIQNYIPPYGTLSRMIEDETRPSLHVLVRREDVDASRLD
                     GRVVVVIDVLFATSTIVHALASGASRVYPAFDAEDARRAAVARPGALVAGEYLAKTLP
                     EHAPATPLALAARLETGRELVYFTTNGTRAIAACEGAAAVYAGALTNAAALAAHIAAT
                     HPRTPVLLVCAGSVGRLCLEDFIGAGHLVRALRDNGDYALTDAALAAAFAVGGADIDH
                     LLTASRVGRMLSRAGMGDEIAHAAACDSSPVVPVLRDGALVRAAA"
     gene            22210..23268
                     /locus_tag="PK399_04275"
     CDS             22210..23268
                     /locus_tag="PK399_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HPW12370.1"
                     /translation="MSAPSLFPDVEGNATRLQAWLQGQLEGFGGAMDIERLHGGRSNP
                     GWRLRTAHRDCVLRARPGPRANLLPSAHAIEREFRVQRALAGSAVPVAAMHALCEDEA
                     IIGAGFYVMDFVDGDILREQALPGLEPSQRAERYDAMNAGIAALHLLDVDALGLRDYG
                     RSGQYFARQIDRWTRQYVASRDGEIADMDKLIAWLPLHVPREEDEPVALVHGDFRIDN
                     MVFARGEPRLLAVLDWELSTLGHPLADFAYHVMSWHIAPTPLSGLGGLDLDALGIPDE
                     QAYVQAYERRTGMKVGGQWDFSLAYNLFRLAAILQGVGARARAGMATDPAALQFAAQV
                     PTLAALGWGFAQRAGSNA"
     gene            23312..>24224
                     /locus_tag="PK399_04280"
     CDS             23312..>24224
                     /locus_tag="PK399_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPW12371.1"
                     /translation="MAVTTTSRDGIAVITFDNPPVNGMGLAVRRGIADAIAAANADDA
                     IAGIVITGGGKVFSGGADIKEFNTPRAEQAPNLHDVIAAVEASPKPVCIAINGTALGG
                     GMELSLGANYRVAHAKAEVGLPEVKIGILPGAGGTQRLPRVVGLETALNMIVSGASVP
                     AANLARTALFDAVVDGDVVDAAVDLLKQKIAGGGPHPRVRDRKVEHDDAEAFIAWARM
                     GVAAASKDFPAPLRCVDAVAASLKPFDAGMAVEREGFTVLLNSPESKALRHAFFGERA
                     AGKIDDIGAEIPPRAIDKVGVIGAGTMG"
BASE COUNT         3789 a         7927 c         8428 g         4080 t
ORIGIN      
        1 acgacaaact aaccgggtcg gtcgtgtgcc tcttccctca gttgggagaa gaaccaaaaa
       61 aaggaggccc gggcaagccg ggcctcctcg cgctaccggt tgcaggcgcg cgtcaccaga
      121 tcacgacctg cttgtcgcca tcgcggacca tcggatcgcc gaccttgcag ccgaaggcct
      181 gggcgaaggc cggcatgttg gacggtgcgc caatcgcgcg gaagttggcc ggcgcgtgcg
      241 agtcggtggt gaggcgcacc ttcagctctt ccgggctgaa gttgcggcgc cacaccgttg
      301 cccagttggc gaagaagcgc tggtcgcgcg tcatgccgtc ggtcttgggg tcgggagtgc
      361 cggcggtcgc cttcttcatc gcatcgtagg cggtggccag gccgccgagg tcggcgatgt
      421 tctcgcccag ggtcagcttg ccgttgacca tcgcaccctt ggccgcctcg tagccgttga
      481 actgggcgac cagtttgtcg gtgaggccgc tgaagccctt ggcgtcggcc ggcgtccacc
      541 agtcctcgaa attgccggtg gggccgaaac ggctgccctg gtcgtcgtag ccgtgggtca
      601 tctcgtgccc gatgaccgcg ccgatgccac cgtaattgac ctcgtcggtc gcgttcgggt
      661 cgaagaacgg tggctgcagg atcgcggccg ggaacacgat ctcgttctgc tgcgggttgt
      721 agtaggcatt gaccgtctgc gggctcatgc cccattcggt cttgtcgacc ggcttgccga
      781 tcttgccgag gttccacttg tagttgaact cgttggcggc cagtacgttg ccgatgtagc
      841 tgtcgcggct ggtggcgagg ccagcccagt cacgccactt gtccggatag ccgatcttcg
      901 gggtgaagct ggcccacttc tccatcgcct ttttcttggt ttcggggctc atccacgcca
      961 ggttctcgat gcgggccttg agcgcctcgc tgaggttctt gaccagggct tccatccggg
     1021 ccttggattc cggcgtgaaa gcgaccttga cgtacatctg gccgagcgct tcgccggcct
     1081 ggttctcgat gacgtcgagc acccgcttgc cacgttcctt cagttccttc tggccgcgca
     1141 gggtcttgtt gtagaagttg tagttctcct gcacgaacgc gtccgacagg tacggcgatg
     1201 cgccgtcgac cgtgtggaag cgcaggtagc tctgccactg cgcggccggc acgtcggcca
     1261 gcatcttgct gacctcggcg tggaactccg gcatcgccag cgagaacatc ttcggcgtcg
     1321 cgacaccctg cgactcgaag aacttcgtcc acgggaaatt gggcgcgagc ttgtccgcat
     1381 cggccgggct gaccgggttg tagaaaatct tcacgtcgcg cgacatctgc tcgctcgact
     1441 tggacacctt ggccaggcgc ttctcgaagg ccaccacgtc ggcggcctgc ttgaccgcgt
     1501 cggcggcggc gatgcccgag agctcaagca ccttggccac gtgggcctgg taggcggcca
     1561 gcttgtccgc cttgtcggca tcctcgtaat agcccttgtc gggcagaccc aggccgcctt
     1621 gggtggcgta ggcgatattg accgtggaat cgttgaagtc tgcgttgggg ccgaagccga
     1681 agaggttgtt ctcgcccttg gccgcgctgg tgcgcaggta ctcggcaatc gcctccttgt
     1741 cggcgagcgc ggcgatcgcg tcgagatggc ccttgatcgg tgtaatcccc tgggcgttga
     1801 tcttcgcggc atccatgccg gtggcgtaca ggtcgccgac gatcttttcg acgccggtcg
     1861 cgtccttcat cgccgcggcc tgttcggcca gctggcgctg cgcggcggtc gagcgctcgt
     1921 cgagcatctc gaatgcgccc caggaggtgc ggtcagccgg gatcgcattg gcggcaagcc
     1981 acttgccatt gacgtagccg ccgaaatcgg tgcacgcgtt cttgctggta tcgaggtcgc
     2041 cgatcaggaa gcggttgacc ggcggcagct ttgattcgtc cagggtgtat gcgggcgtct
     2101 tggtttcggc agcagccgtg cttgcggcag gtgcctcggc cggcttctgg cagccagcga
     2161 gcgcggcggc gatggaaagg gacagcaaca aggtcttcgg ggtattcacg ggatacgact
     2221 ccgggcaatg cggggaaaca acgggagcta cgcgtggggt cgcgcagcga atctggcgag
     2281 tggtcagtgc ggtgaactca aggcgagaag cgcgggtcgg tcagcttcat ctcggtgagc
     2341 ggaccgtagg ccttcatcca gtcgcggatc ttcgacgcat cgccgatggc cacgatggcc
     2401 aggtccttgc tgtccgggaa caccatgcgc gcctcggcaa tctgcgccgg ggtggccgcg
     2461 gcgatcttgc cgaggtagcc gtcgacggcc tcgcgcgact cgccatacag ggtcatgtcg
     2521 accaattgcg cggcgatctg cggcgcggtt tccaggccgg gtgcgtattg cccggcgaca
     2581 tagcgcttgg ccgactcgat ggtggccgca tccagcccgt ccctgtgcag gcggtcgagg
     2641 gtggcgatgg ccaggtcgat cgcggccttg gtggtttcgg tcttggtgaa gctgctgatc
     2701 accgcagggc ctggctgtga ggcacgttcg atgcccgagc gggcaccgta ggtcagcccg
     2761 gacttcaccc gcagctcggt attgagcatc gaggtgaagc gtccgccgaa cgcggtctgc
     2821 accaggtcct gcgcggcttc ggcgggatcg ccacgcttgc tgccgacatt ggccagcgcg
     2881 aagtaggtct gggtcgcgcc cggcttgtcg accagcaaca cgcggcggcc ggtttcgcgc
     2941 gccttggctt cgacctgcgg caaggtgcca gtcgccttcg cccatccgcc gaaggactgc
     3001 ttcagctgcg cggtcaccgc cttggcatcg aagtcgccgg cgatggcgat gaccaggcga
     3061 tcgccgccca tctgctgctg gcggaaggcc tgcaggtcgg cgagggtgat cgtggccagg
     3121 ctggtctcgt cgccaccggt ggagcgcccg taagggtggc cgcggaacag ccaaccgccg
     3181 gaatagagcc cgatcagctg gcgcgggtcg gagtccttgg cattggcgat gccgtcgatc
     3241 gcgcgcttgc gcagcttgtc gaactcggcg ctgtccatcg tcggccgcag gagtgcgtcg
     3301 gcgagcagcg acagcatcag cgcgctgtcc ttcgacagga actgcgcgtt ggcagtgatc
     3361 gcttcgcgcg agctgccgaa gccgatgctg ccaccggcgc catcgacggt ctgggcgaac
     3421 tgcagcgcgc tgcggctgcc ggcgcccttc gacagcattt cgccaaggat gtcggcggtg
     3481 ccttccttgc cgggcgcgtc ggccagcgcg ccgccgcgca cggcgatgtt ggcggcgatc
     3541 agcggcacgt ccttgcgcgg catcagcagc acggtggcgc cgttgtcgag ggtgatagtc
     3601 tggtagtcgg gcagcttgac acttggttca gacgccgcgg cggcgattgc gccgttgaac
     3661 gcgaacgccg cgatcaacgc gatggaaacg ttgcggaaga agctcatttc gcggcctcct
     3721 tgtcggcggc agtgggcgcg gcggcgaccg gcttcagcaa gccggtggtg cggttctgga
     3781 tgcgcaggat cttcttcgcc agcgcctgca cgtcggcagc gctgaccgcg tcgtaggcgg
     3841 cgggtgcgtc gaacagcttg cggtagtcgc cgttgaagac ttcataggtg ccgagcgcgc
     3901 gcgccttgcc gttgatcgtg gccatgccgc gccagaaggc agaagacttg aggttgcggg
     3961 ctttctcgag ctcggcggcg ctcacgccgt cgcgcgcgat cttcgcaagc tcgtcgtcga
     4021 gcagggtctc caccttcgcc atgtcgccgc ctggcggcag catcgcgtag atccacagca
     4081 ggcccgggtc gaagccggcc tccgcgaagg cacctgcctg ggtggcggcc tgttccttct
     4141 cgaccaggcg ctggtgcagg cgcgaggact cgccctgcga gaggatcgtg gtcagcaact
     4201 cgagcgtcgg gaattccttg ctgccggcgc cgaggccact gtggaaggcg taggcgacga
     4261 tcggcgattg cgcgtcggcg cgttccacca ccagccggcg ttcgccctgc tgttcgggct
     4321 cgacggtggt gattggcgcc ggcgcgggct gcttcggcag cggtgccagg tacttgtcgg
     4381 cgagcgcgaa cacctgcgcg ggatcgacat cgccggcgat gaccagcact gcgttgttcg
     4441 gcgcgtaata ggtcttgaag aacttcttca ggtcgtcgat cttccagccc tcgatgtcgc
     4501 tgggccagcc gatggtcggg aactgatagg gatgcgcggt ccaggcggtg gcctgcatct
     4561 gctccatcag cgtgccgaaa ttgtcgttgt cgatgctgga gcgacgctcg ctgaagacca
     4621 cgccgcgttc gctctcgatc accttcggat cgaacgacag gttggccatg cggtcgccct
     4681 cgaggtcgaa gatcgtctcc agcgcgctgc tcgggaacca gtcggtatag atggtcaggt
     4741 cgttgctggt gctggcgttg ttgctgccgc cggccgcttc catctgcttg tcgaactcgc
     4801 ccggcgcacg cttcgtggtg ccgttgaaca tcatgtgctc gaagtagtgc gccaggccgg
     4861 tgatgcccgg gtattcgttg cggctaccgg ccttgtagaa ggtgtagtag ttggcgttgg
     4921 ggatgtcgtg gtcgggccag accacgatct tcaggccgtt cgcaagcgtc ttgctgacga
     4981 tggcctcgct gccggggacg ttggcggctg gttggggtgt cgctgcttgg gccgcaacgg
     5041 agctcaggcc aagcgcgatc gcgcaggcca gtgacaacag gcgcatgggt gttccctcgg
     5101 atgggccggg cgaatcccgc accatgcacg aaacaccctg tccccggcac ctgccggaag
     5161 tcgtggccgt ggccctgccg aatccgttcg attccgtcag cgcgacccga aggcgtgcca
     5221 gagcggcgtg cggatgcgca acgccaccag cgccaggctg ccgatcagca gcacgctgta
     5281 ctccatgccg ttgcggccgg cgccgaccac gaaccagcct tctggcgcgt gcaccagcac
     5341 gatgcccatc gcgtagatcg ccatgaacaa cagggcgaac gggcgcacgc acaggccgac
     5401 ggccaggcag gcggagccga tgatctcgct gatggtcacg ccccaggcga tcgcgtgggc
     5461 cagcgggatg tcctgcgtgg ccagccagtc cccgaagatc ggcgaggtgc cggcatgcag
     5521 gcgatgccag ccatggatcc cgatcagcag ggcgacgagc aggcggaagc cgagcagcgc
     5581 gcaatcgggt cgtgctccgg caggacgtgc gtggttcatg tcgggtcggc ctcgcgggag
     5641 gacgggatgg gacgggacgt gcgcgtcgcg tcatcggcgg cgatcaccaa cagcatcgcc
     5701 agcagggcca cgctgtattc gctgccgccg gtgccgtgct cgccgacgaa ccagccgttg
     5761 tggcgatgga tcagccagat gcccgtcgcc acgatcaccg ccaggccgct ggcggcccag
     5821 cgcacgcccc ggttgaacaa caagaggctt ccggcaccca gctcgtacgc ggtgatcgcc
     5881 aatacccagg cctgcccgtg cgggaagccc tgcgactcca tgaacacgcc gaactgcagg
     5941 atcgtgccca gcacgaaacg catcgaggca tgcgccatga acagcagggc agtgacgatg
     6001 cgcagcaggc gcagcgcatc ggccagcggc caccagggat agcccgtggc cagtcccgtg
     6061 gacgcgcgaa ggaaaaagac gcgggacgat tccggcttgt cccgcgttcc caggggtcgg
     6121 gtggtggcgc ccatgcgctc agaacgcgta ctgcagcatc acgcgcaccg cgcgtggcgc
     6181 ctggaagccg gtcgggatct tgtagacggt gtcgtacagc gagctgccct ggccggtctc
     6241 cgcggtttcc accacgctgg tcaccgtgcg cgagttgaac acgttgaaga catccagctt
     6301 gagggtcagg tgacctgcgg cgaacgccgg tttccaggcc acgttgagat ccagctggcg
     6361 ttgccacggg gtgcgtccgg cgctgccgcg cgggaccagg gcggtgccgt tgtcgagcac
     6421 gccatcgccg tccgcatcgg taccgggcgc atcgctgcag gagaagtacg aggcgccgta
     6481 gtgcagcggg tcgttgccgt acacgccgaa gcagttgatc ggccggccgg actgcaccag
     6541 caggttgccg ccgatcgacc actggtcgtt gagttcgtag ttgccgaaca ctttcagcgt
     6601 gtgcttgcgg tcgttcggca gcgggccgtc cgcgcccagc atcaactcgc ggtagtcgaa
     6661 gtcctgggtg gtgccggtgt cggcctggcc gatgtcggac ttgatgccgc cctcggtgtt
     6721 gccggtgctg cgcgcccagg tgtaggaacc ctgcaggaac agcttgtccc agtggccttc
     6781 gaagaagaac tccgcggccg cgtaggtgcg cttggccttc ggccccagcg tgtcggcgtc
     6841 gatggtgact ttctccagcg tcccgttgcc gtcgatgtcg atgttgaaca cgccgtcgct
     6901 gcccgggttg tagaggtggc agaaggtgaa gcccgggttg atgaaggcga tctccttcgg
     6961 caccgtgctg tccgccggcg actggaaggg atcgccgtcg gggtcgacga agccgttggc
     7021 gatcgcccag tcgtagatcg cgcgctcgtc gcagttgtcg tcgatcgcgc ggcgcaggtc
     7081 gcggaacacc gtgcgcacgc cgaacgacca gtggtcgtcc acctgcttct ggaagcccag
     7141 gatgaattcg tcctggtaca tcggctgcag gttttccgag gcgatggtgc gcggatcctt
     7201 ctgcgcaccg aactcgttgt tgaggtaggt gatcggggtg aacggggtcg cgccgacagg
     7261 cgcgccggtc gcgggatcca cgctgtcgta gaagaacaac tgctggctga agatcgaggc
     7321 gctggcgcca cgaatggcga ccgtcgcagt cagcggcaac gcgtaacgac cggcattgcc
     7381 gaacaccttc agcgaggcat cgcccttcac gtcccaggcg aagccaagcc ggggccccag
     7441 ctggttgtcg atcgacgcgt aggactcgcc gttgccgttg cggttgtcga agctgtccca
     7501 gcgcaggccg gcataggcca ggaagttcgg ggtgacctgc cagcggtcct cgatgtagaa
     7561 cgcgcgctgg tcgacgtcca cggtggcgcc gttcttgaac accacgttga ccacgaagtc
     7621 gaggtcgtcg tcgttcagcg gcacgccgtc gggatcgatg gtcgcgtagt tccagacctg
     7681 gccgccttcg atcgcgccgc cggcaacgga ggtgaagcga tcggcgtcgt agccgaagcg
     7741 cagctggtgg tcgcccaatt gccagtccac atcgacgcgg aactgcctgc gggtatcgcc
     7801 ggcatccggg cggcccaggg tgccgccgat gaaatcgcag ccgcggatcg acggcgtggc
     7861 gccaagcagc gtggagttgc gctggtcgac gatgaccggg cagccggtgt ccggcgcgtt
     7921 gatgtcgccc gaccattcct ggcgcgtgcc gttcgcggag atgccgtaga tgctgcggct
     7981 gaacttgccg cggccgaaca gcgcggacag ggtgaagctg tcgctgaggt agccggtgta
     8041 cttcagcgcg tggctggtac cgccctgctc ggtgtagacg gttccaagca cgcccgttct
     8101 gttgagcacg ccttcgtcgt tggtgtgcac gacctgggtg gtgcgggcca cgtcggagaa
     8161 gctggtcagc tccagcaagt ggtcctgcgc caggttccag tccatcttca gcaaccaggt
     8221 cggcgaccgc gtgcgcgtat tcgcattgat gccggcgttg acgtcgttcc aggcatcgcc
     8281 gtggttgcgc gcgaactgca ccaggccgta ggcgaacagg cggtcgcgga ccagcgcgcc
     8341 gccggcccac agcgcgacgc tggattgttc cgcctcgtcg cgtgagttgt cctgcagcag
     8401 gtcgccattg ttgaggaaga tgtccggcgt ctttccggac agcgaggagg gcgtccagta
     8461 gagcgcgccg ccagccgaga aatcgttggt gccgcgacgg gtgatgaggt tcaccacgcc
     8521 gccggtggag cggccgaatt cggcgccgta gccgccggtc ttgacttgct gctcggcgat
     8581 cgcctcgaac gggatctgcg cgaaggcgag gttgttgaac gcgttggtga tgttgaagcc
     8641 gttgacgtaa tacgcgttct ccgccaccga cgagccgcca aacgaggcca ggttgccgaa
     8701 ggcggcgtcg ccacgcaccg tgccgggtgc gagcagggcg acgctggtgg cgtcgcgcgc
     8761 caccgggatg ctggccatct gttgcgcgga caggatcgtg gtggattcca ccgacgacac
     8821 gtcgacagga ttgatgctgc ctgcgcgcac cgtcactgcc tccagcgtgg tggtgtcgtc
     8881 ggccgagaaa tcgacctggg tgccggtgcc cacgctgacc gcgacgcgct cgcggacccg
     8941 cgtgctgcca tcggcgcggc gcacggtcac cgtgtaatta cccgaaggcg tttgcggcgc
     9001 gcggtagcca ccatcggcgc cgacggtgat ctcgcggcgg tagccggtgg cggcattctc
     9061 gatcaccacc acgtcgccct ggctggcgcg gccgtggatg gcgccggcgg cattgctctg
     9121 cgcggatgcg agcccggcga tgcccaggcc aatggccagg gcgaggcaga gggtggtgtg
     9181 cttacagtgg ttggcgtgtc gtgcggctgg catgtggaac tccccggggt ttcttggttg
     9241 cggcagtggc cgtggggcga cgctcgcggt cgcgcggggt gtgcggcaag catccgggga
     9301 cgaaacgcac gcgtgcggca gccaacgaca cccggcgcgt tgcgggtggc cgtgcccgcg
     9361 ggtagcgtgg gggcatggat cgcgccgaag acacccgctg gttgccgttg ccgccccgac
     9421 cgtggtggac gctgtggctg tccgccttcg ttgcctggac cgcatacggc ctgctgtact
     9481 gctaccaggt gatgtcgatg gccgaggaca tgggcaagca ctacgcatgg agcgatgtgc
     9541 tgcgcatcaa cctgatcgga atgtggggct gggtaccgct gaccgtcgcg ctgttctggc
     9601 tggtgtcgcg cgtgccgatc cggcgcggca gcgtggcccg gtcctgcacc atcctgatga
     9661 cggctgcctt cgcggtggtg gtgctgcgct cggtctacat ctataccctc gatccatggg
     9721 tcggctggta cgacaccccg ccggatttct ggtccgatgt catgccgacc agcctcaaca
     9781 acaacgtgct gatcagctgg atcgtgatcg gcctggcgca tgcgctgctg ttctcggacc
     9841 aggcgcgcgc gcaggaacgt cgcgccgccg agctgcaagg cttgctggcg caggcgcggc
     9901 tcgatgcgat cgcggcgcag ctcaacccgc atttcctgtt caacgcgctc aactcgatcg
     9961 ccgaactggt ccaccacgat gccgatcgcg ccgaccgcat gctggtcggc ctgtcggcac
    10021 tgctgcggcg cagcctggac agctcgcggc tgcaatgggt cagcctgcgc gaggaactgg
    10081 aattgctggc gcaatacctg gagatcgaga aggtgcgact gggcgcacgc atgcaggtgc
    10141 agtggaacgt cgccgaggag tgcctggacg aggcggtgcc gccgcagctc ctgcagccgc
    10201 tggtggagaa cgcggtggtg cacgccatcg cgcggcggat ccgtccgggc agcgtgcgca
    10261 tcggtgcggg cctggtcggc gctgcgctgt ggctggacgt ggaggacgac ggtgccgacg
    10321 ggctgccgga tgtcgagggc cacggcatcg gcctggcgaa tacgcgcgcg cgcttgcagg
    10381 tgctgtacgg cgacgaggcg gtgctgtcga tcgaggcgac ggccaacggc ggcacccgcg
    10441 tgggcctgcg cctgccgcgt cggcacgcaa tccccgccag cacccgcgat cgcccagcac
    10501 gcgacctccg cttcgacgag gtgcgggtgc catgagccaa ctgcgggtga tgatcgccga
    10561 cgacgaaccg ctggcacgcc tgcgcctgcg tcgcttgctg ggcgggatcg ccggcctcga
    10621 ggtggtggcg gaatgcgcca gcggcatcga ggtcatgcag ctactgccgg actgtccctg
    10681 cgacctgctg ttcctcgata tccagatgcc gggcgcggac gggttccagg cactggagca
    10741 cgtggtcgcg cggccacgcg cggtggtgtt cgtcaccgcg tatgcggaac acgcggtgcg
    10801 cgcgttcgac gtgcaggcgg tggactacct gctcaagccc atttccgccg agcgcctgct
    10861 gcaggcggtg gaacgcgcgc aacgcgcgtt ggccaacgat gcgcccctcg ccgcgccacc
    10921 ggcgattcca cggcggctgg cgctgccgat cggccggcgc atgcagatgg tcgagctgga
    10981 cgagctggat tgcgcgctgg cgcaggccaa ctacgtcgag ctcaaggtcg gcacgcgcag
    11041 cttcgtcctg cgcgacacca tcagcggttt ccatgcacgc ctggacccgc agcgcttcct
    11101 gcgcatccat cgctccgcga tcgtgcgcat cgacgcgatc cgcgaggtcg aacctctcgc
    11161 ctccggtcgt tatcgggtga acctgcacgg tggccagcgc ctggtctccg ggcgcagcta
    11221 ccgcgatgcc ctgcgcgcgg cgctggggct ggcacccgag gcgtcgctgc catcgcggat
    11281 cagcgcgtga ccaggcgtcg ccatccggca ctgcggtaac ctgcacgccg gttttgacgg
    11341 agcggacgca tgcgggtgca tttccacggc gcggccggcg aggtcaccgg ttccatgcac
    11401 ctggtcgagg ccgccggaaa acgcgtgctg ctggattgcg ggatgcgcca gggcagcgcg
    11461 gaaatggagg ccggcaacgt cgatccgttc ccgttcgacg tggcgtcgct ggatgcgctg
    11521 gtgctcagcc atgcgcacat cgaccacatc ggtcgcgtgc cgttgctggt caagcgcggc
    11581 ttccgcggtc cgatcttcgc ccagcaggcc accgccgacc tgatgccgat catgctgctc
    11641 gattcggcat cgctggccga aagcgatgcc gcgcggttca accgcaggcg cacgcatggc
    11701 gaagtggaac agcagccgct ttacacgaag gacgaagtgt ccgatgcgat ggcattggtg
    11761 cgcgcattgc cgtacgacgc gcgccagccg atcgtgcccg gtatcgagat cgcgttccgc
    11821 gatgcagggc acatcctggg ctccagcatc gtcgagctgt gggcggatgg gcgcaaactg
    11881 gtgttctccg gcgacctcgg cccgacgggc acgccgatcc tgcgcgatcc gacgccgatc
    11941 cgggacgcgg atctggtgct gatggagtcc acctacggcg accgcgacca caaggatcgc
    12001 gcggagacga tcgtggagtt gggcgggatc ctggagcagg catggcgcga cggcggcaac
    12061 gtgctcatcc cggccttcgc ggtcgggcgt tcgcaggaac tgctgtactg gttcgcgaag
    12121 tactgggacg agtgggacat gtcgcgctgg caggtgttcc tggacagccc gatggcaggc
    12181 aaggtcgtgc aggcgtacgg gcgccaccac gatctgttcg acgacgaggc gcgcagggtc
    12241 tggcgcgatg cgccgaaccc gttcaagttg cccaacctgc acatcaccgc gtccaccgag
    12301 gaatcgatgg cgatcaaccg cgtccagcgc ggcgcgatcg tcatcgccgg cagcggcatg
    12361 gccaacggcg ggcgcatcct gcaccacctg cgcaacaacc tgggccgtcg cgaggcgcac
    12421 gtggtgttcg tgggctacca gggcgccggg acgatcggcc ggcggttggt ggacggggcg
    12481 agatggatcc gcatccacgg tcgcgatatc cgggtcaacg cgacgatgca caccgtcggc
    12541 ggcctgtccg cgcacgccga ccagcgcggc ctgctggcgt ggttcggtca tttcgatccg
    12601 aagccggcac tcgcgctggt gcacggcgag gacaaggcgc gcgaagcgct ggccggcgag
    12661 atcggcgaac gccacggcat cgaagtggtg ctggcgcgcc cgggcatgac cctcgacgcc
    12721 tgatgttcgg gttcgggcgc agcgcgacct tcctgctgct ggcgctgctg gcggaaatcg
    12781 ccgggactgt cggcggcttc ggctcgtcgg tgttcttcgt gccggtcgcc aacatgtatt
    12841 tcgatttcca gtcggtgctc ggcatcaccg cgctgttcca cctggccagc aacatcagca
    12901 agctcggcct gttccgcggc ggcattgatc gcgggctgct gctgcgcctc ggcgtgccat
    12961 cggtggcgtt cgccgcgctg ggtgggctgg ccacgacctg gttccccacc cgctggctgg
    13021 aggccgtgct ggcggtgttc ctgttgggcc tgagcggctg gatgctgctc aggccgcgct
    13081 ggcgcctggc gccgacgacg cgcagtgcgg tggtcggcgg tgcgttgtcc ggtggcatgg
    13141 ccgggctggt gggcacgggc ggcgcggtgc gcggcatgac actcgcggca ttcgacctgc
    13201 gcaaggaggt cttcatcgcg acctcggccg ccatcgacat ggcgatcgac ctgtcgcgcg
    13261 ccgcggtgta cgcacgcaac ggctacatcc atgcccacga cctggcctgg gtgcccttgc
    13321 tcgccgtggt cgcgatcacc ggcacctggg tcggcaagcg catcctggcg cggatgccgc
    13381 aggtgctgtt ccgccgcatc gcgctgctgc tggtgttcgc gatcgggctg gtgagcctgt
    13441 tccaggccgt tcgcgcataa cccgacacgg agacccatgc catgagcgaa ctgaagacac
    13501 gccccaccgc ggcatcggtc gccgaattcc tggcgcagca gggcgaccag cgccgcgccg
    13561 attgcgaagc ggtgctgcgg atgatggaag ccgtcaccgg cgagcgcgcg gaaatgtggg
    13621 gcgcggcgat cgtcggcttc ggccgctatg cgtacagagg caactcgaag aaggccatgg
    13681 aatggccgat catcgggttc tcgccgcgca agaacgacct gaccctctac ctcatgcccg
    13741 gcttcgaagg ccgtgccgaa ttgatggcca ggctcggcaa gcacaagacc ggcaagagtt
    13801 gcctgtacct gaagaagctc gccgatgtcg atgcgcgggt gcttcgggaa ctgatcgatt
    13861 gttcggtgcg gggaatggac ccgcagcgcg tgcgtcgttg aacgctgccg tcaggcttgc
    13921 tgcagcgccc gccattccca cagggccacc agcaccagag tgccgctggt caatccgccc
    13981 agggccagcg cgctgaatgc gtggccgaag gcgaacggcg acagagcgag cagcaacccc
    14041 atgcccacga gatgcgagat cggcggcaac cggcgttggt tggtggccca tttgaacaac
    14101 ccgttgccga gcaggtacag gaacgggccg cccaggatcg tcgcgatgcc cgcgttgctg
    14161 gcgtggtcgg gatgcaccag caccagctcg tccgcgaccg cgcacaggat gatgccggcg
    14221 acgatcggga tgtgcaggta ggtgtaggcg agccgcgcca cgcggccgcg gtccgcggca
    14281 tgttcgaagt ggtggatggc gcgcccggaa ccggtgtcga agcacagcca ccacatcgcc
    14341 accgtaccga cgaaggccgc gatgaatgcc agcagttgcg gcgtctccca ggcgaggccg
    14401 gcgaaggtcg cgccggtgat cagcaacgat tcgcccaggg caatgatcac gaacagtccg
    14461 cagcgttcgg ccatgtggtg cggatcgacg tcccagtcgg tcgtcgtcga ggcgcccatg
    14521 ccgggaacgc ggtagtaggc aaacgggccg atgaactcga tggcgatcgc cgccgtccac
    14581 caggccagcc gcgattgcgg atcggacaag ccgccgagta cccagcaggt gccggatacc
    14641 gccagccacg cgaagatgcg gatgaaattc cggccatgcc cgctgaccgc gccgaacgca
    14701 tgcaccatgt aggccgagcg cagcaggtgc tgggcgacgt aggccagcgc gaacaccagc
    14761 ccgcgctccg cgaacgcatg cggcagcgcg gccgccatca ccagtgccag caacatcagc
    14821 acgaacagca actggcgcac cggggtcgcg gccgggttga gccagttggt gcaccaggtg
    14881 gtatagatcc acagccacca cgcaccgatg aagagcaccc cggtgtgcag cgcccccagc
    14941 ggcgtcagtt gttccagcaa gccgtgcgag agctgggtga tcgcgaacac gaacaccagg
    15001 tcgaagaaca gctcgaccat gccgacctgg ctgcgttcgt ggccgcccgc gctgcgcagg
    15061 gttggctgac gattcgcccg catgctcagc ggcgtttgcc cgtgaccacc gtggacagca
    15121 ggaacaggcc gcccacgaag ccgatgtttt tcatgaaatg cagcagctgt gcgccctgtt
    15181 cggcgccctg cagggtccag aagcggtgcg agagcacggc atcgacgagc atcagcaggg
    15241 cgcccagcaa cgccatcgcg cgcagtcgcc aaccggcggc gatggcgatg ccgagcagga
    15301 attcgccggc actgaaggcg agggtggcat tggcggtcgg cacgccctgc agcgcttgcc
    15361 acaggcggta gccgcccatc acgaggaaga cactggccat cagccagctg gcgatgcgga
    15421 tctcgggctt catctcgatg ccggttgcga tgtgaaggcg cgagcgtcgc atggtcgcgc
    15481 gcgggcgtga aggggcggca ggtgaatccc gcattcatgc tggatcgacc gggtgggcga
    15541 tttcgcgtat catccgactc gttctgcgca cggctgatcg ttttcccggt cacgcctgcc
    15601 gcctcggcgg cctgcttctt ccacacgcct ttcgtcgcgc agacacggca ccggatcacc
    15661 ggcgtcgtgc catcgtcgac tcgcagcgcg gttccaggga aacgctcggg ttcgtccacg
    15721 ccattgcgtg ggcttttcac accggagcat taccaaatga cgtttgaatc cctcgggctt
    15781 gcgcccgcgt tgctgcgcgc gctcgccgac aacgactaca acaccccgac cccgatccag
    15841 gcccaggcga tcccgctggt gctgtccggc cgcgacgtgc tgggcggcgc ccagaccggc
    15901 accggcaaga ccgccgcgtt cgcgctgccg gtgctgaatc gcctgtccaa ggaaaccccg
    15961 ccggccggcc cgcgcaagcc gcgcgcgctg gtgctggtgc ccactcgtga gctggcggtg
    16021 caggtcgccg acagcttcaa gacctacggc cgtcacctga agctcaacgt gaccacgctg
    16081 ttcggtggcg tcggcatgca gccgcagatc gagcagctgc gccgtggcgt ggacgtgctg
    16141 gtggcctgcc cggggcgcct gatcgaccac ctggaccgcg gtagcgtgaa gctggatgcg
    16201 gtggaagtgc tggtcctgga cgaagccgac cgcatgctcg acatgggctt cctgccggcg
    16261 atcaagcgcg tgctcaaccg tttgccgaag cagcgccaga ccctgctgtt ctcggccacg
    16321 ttcgaggcgc agatcaaggc cttggcgctg gagttcatga ccaatccgga acaggtccag
    16381 gtggcggcgc agaacaccat cgcccagacc atcacccaca ccgcgcatcc ggtcgacggc
    16441 tcgcgcaagc gcgacctgct gatccagatc ctgtcgcagc gccacaccga ccaggtgctg
    16501 gtgttcggca agaccaagca cggttgcaac cgcctggccg agcagttgga agaggccggc
    16561 ctcccgtcgg tcgcgatcca cggcaacaag agccaggccg cgcgccagaa ggcgctcaag
    16621 gacttcaagt cgggcaaggc gcggatcctg gtcgccaccg acgtcgccgc gcgcggcctc
    16681 gatatccccg acctgccgct ggtgatcaac cacgacctgc cgatggtcgc cgaggactac
    16741 gtgcaccgca tcggccgcac tggccgcaac ggtgccgtgg gccaggccct gtcgctggtc
    16801 tcgccggaag aaggcggcct gctgcggcag atccagcgcc tgctgaaggt cgacctggtg
    16861 atggacgtgg tacccgggtt cgcgccgagc aagccgatcc gcctggatac gccgatcccg
    16921 aagaacggcg gcaatggcgg ccagcgtccg ccgggtcgtc cgggcgctgc cccgcgtccg
    16981 cacgcacgcc cggcggcacg cgccggccac gcgggccagc agcatcgccc gagcgggcag
    17041 ggtgcctatg ccggcaagcg caacgcgccg cgcggcacgc gcgcctgagc cacgcgacat
    17101 cgcgatagcg aagggccggc attgccggcc cttttttgtt cacgggcgcc aagccattgg
    17161 ccaaggcgta attgctactc ggctcctgcg aacatcctgt tcgagtcgcc gccgcgcgcc
    17221 cacttcgtgg cccagcccgg cacagggtgc cgggcgttcg cgaactcgca tcgcatgcga
    17281 tgcaagcaga gcgcgctcac ccatggcgcg ctttccgcaa tcacgccttg gccaacggct
    17341 tggcgtcaga gttctcagcc ttccgcagcg tgctcgcgca attcgtccgc acgtgcacgg
    17401 ccgaggtagc gatggatgcc ggcgcgcagc aggtacagca gcggaagcag tgcgatcgcc
    17461 gcgagcatct tgtagacgta gttgagcgtg ccgaccgcga ggaacagcga ggttggccac
    17521 tgctgtggac ccagcacgaa ggcgatgtac agcaccacga aactgtcgac gagctgcgac
    17581 accgcggtcg agccggtggc acgcagccac gcgtggcgct cgccggtgcg cgaacggatg
    17641 cggtggaaca cctgcacgtc gatcagctgg ccgaccagga aggcgatcag cgagccgacg
    17701 atgatccaca tgccctggcc gaagatcgcc gcataggcgg cctggtagtc gggcacgccc
    17761 tggtccttgg ccgcacctac ccaccacgaa gccggcgcca ggtggatggc gaggtaggcg
    17821 aacacgaagc tgtagacgat caggcccgcg gccagccagc tgatcatgcg gacgccgcgc
    17881 ttgccgaaat actcgttgac cgtgtcggtc atgatgaaga cgatcggcca cagcagcgtg
    17941 cccgcggtga agttcagcga gccgctctgg ccgaacaggt tccactccag cggcgcgatg
    18001 cccaggctgt cttccagggc gaagatcttg accccgatga attcggccag caccgcgttg
    18061 gcgcagaaga atgcagccag ggcgacgaac aggcgggtgg cgcgatcatc cagcaggcgc
    18121 agcggcgaaa cggaagcggt cgtgtccatg cgccagagtg tacggcgagt cggccggcgg
    18181 tcgtgtccga accgacgcgc gcggcttacg gcgccagcga cgggacgatg accagtttgc
    18241 cgatcgcgtc gcggccgagc agcgcatcca acgcctgcgc ggcaccggac aacggatagc
    18301 gcgccgagac cagcgggcgc aaggtgccgt cgtgcaacca cgccagcagt tggcgggtgt
    18361 tggcggcatg ggcctccggc tcgcggcgca cgaactcgcc ccagaacacg ccgaccagcg
    18421 cgctgccctt gagcagcagc aggttcgtcg gcagcttcgg gatgccgccg ccggcaaaac
    18481 ccaccaccag gtaacgcccg ccccagccga cgctgcgcag ggcggtttcg gcgatgtcgc
    18541 cgccgaccgg gtcgtagacg atgtccacgc cgcggccgtt ggtgagggtc ttcaactgtc
    18601 cacggaagtc gtccgtggtg tagtcgatgg cctcgtcggc gccgcgcgag cgcgccgctt
    18661 cacgcttggc ttcggtcgat gccgcggcga tcacccgcaa gcccatccgc ttgcccagtt
    18721 ccacggccgc caggcccacg ccgccaccgg caccgagcac cagcaaggat tcaccgggtt
    18781 tggcagccgc gcgatccttc aacgcgtggt acgacgtgcc atacgtcagc aggaaactgc
    18841 cggccagttg cagttcgtcg tcgctcgtat cgttgggcaa gccaatgacc tgcgcggcat
    18901 cggccaacac ctcttccgca tacgcaccga acgccgtggt gccgaccacc tggtcgccga
    18961 tcgcgaacga cgacacgcca tcgccaagtg cactgaccgt cccggaaaac tccgcgcccg
    19021 gcgagaacgg gaagtccggc ttgaactggt acttgccggc gaccagcaag gcatcgggga
    19081 aattcaccgc tgccgcgcgg acgcgcacgc ggacctgtcc cggcgctggt tcgggaagct
    19141 gcagatcctt cacttgcagg gtgtcgggcg tgccccaggc gtcgcagacg acggctttca
    19201 tcgatgcact ctccgttcga tcagaaacgg ccttcggtgg cctcgtacac gcggttgcgc
    19261 agggcgacca ggcgcgggcc gaagtcctgc acgagtttct cgcgggtcat taccgagacg
    19321 cgtccgctga tgttgaaggc gaaacggcgc gtgcgatccg ccgagatcat cggtacgccc
    19381 accgcaaaga cgtcggggtt ccagtcgccc agcgagaaca cgaagccgta ggtctcgtag
    19441 tcctggcggg cacgttcgat gccggcgcgg atctgcgccc acccggccgg gctcgaggtg
    19501 cgctcgagtt cggccaggta gccgtcgaac gcgtcgcgcg agcgcgaggc caggtcggcg
    19561 cggcccagcg cagtggtgcc gtgcggaacc cgcgccccct tctccaggcg catgttgaac
    19621 atcgcatcgc cctggcagac ttccagcagc agcatgcgca tgccctcggg caccgccagc
    19681 atcaccgcgg cctgcgtgta gtcggccagt tcctgcatca gcggttgggc aatgccgatg
    19741 acatcgtggc cggccatgta ggcatgaccc aagccgagca ccgccggccc cagcgcgtat
    19801 ttctccagcg tgggcgaata ctccaggtaa cccagcgtgg ccagcgtctg cgacagccgc
    19861 gataccgtgg ccttgggcag tcgcgtgcgc ctggcgatct cctggttgcc caggtagcgg
    19921 tgttccggat gggaacgcac ctgttcgaag cagcgcagga tcgagaacgc gcgcgccacc
    19981 gaattcacca actgcggatt tcggccctcg gcgtcggccg ccagcgattg caggtcgcgc
    20041 ttcgggttgc ccatcaggcg gcgtccttgg tcgcgtattt cttcaattcc agcttggcga
    20101 cggtgtcgcg atgcacttca tccggcccat cggcaagtcg cagggtacgg ttgtgcgccc
    20161 acgccttggc caggaagctg tcctggctca cgcccatcgc gccatgcgcc tgcaccgccc
    20221 aatcgatgac tttttccgct gcctgcgggg cgatcacctt gatcatcgcg atctcggcac
    20281 gcgccgcctt gttgcccgtc gtgtccatca tccgcgcggc attcagcacc atcagccgcg
    20341 cctggtcgag caggcaacgc gactcggcga tccgctcgcg ccagatcgac tggtcggcga
    20401 tcgcgcggcc gaacgcgtgg cgcgaggcca gccgccggca catcgcctcg agcgctcgtt
    20461 cgcccatgcc gatcgcacgc atgcagtggt ggatgcgccc gggtccgagc cggccctgcg
    20521 cgatctcgaa gccgcggcct tcgccgagca gcatgttggc ggcaggcacg cggacgtcga
    20581 ccaggtcgat ctccatgtgc ccgtgcggtg cgtcgtcgta gccgtacacg tccatcgcgc
    20641 ggttgacgcg gatgccgggg gtatcggcgg gcaccacgat catcgaatgg cgctggtgcg
    20701 gcgctgcgtt ggcatcggtg atgcccatga cgatgtagac cgcgcaacgc ggatcgccgg
    20761 caccggaggc gtaccacttg cggccgttga tcacgtagtc gtcgccgtcg cggcggatcg
    20821 tgcattgcag gttggtggcg tcggacgatg ccacggcggg ctcggtcatc aggaaggcgg
    20881 agcggatgcg gccgtcgagc aagggttgca gccagcgcgc ctgcaatggc tcggcggcgt
    20941 atctcgccag tacctccatg ttgccggtgt ccggcgcgct gcaattgaac acttcgggcg
    21001 accagtacat gcggcccatc aactcgcaca gtggcgcgta atccaggttg gacaggcctt
    21061 ccggcgcatg cgtggagttg ggcaggaaca ggttccacag gccctgcgcg cgcgcctctg
    21121 ccttcagccc ctcgatgacc tcgctgggtt gccaagggtt tccggccgcg cggttggcgg
    21181 cgtcctccgc ggcatgggcg gcctccatcg gccagacgcg ctcctgcagg aaggcgtgca
    21241 gtcggcgatg caattcaacg gcgcggggac tgggcgtgag atccatgtgt ttcactatgc
    21301 ggaacattgg ttccggtggc gatgggatcg tatggcgcat tcggtgtggg tgcaagtcaa
    21361 tgatattctt cgatgcgtag cgaaatccgc attgccttcg gcttgcggtg tcggggctga
    21421 cggtctagat tattccaaaa tacagaatta tattccacca tacggaacat tgagtcgcat
    21481 gatcgaagac gagactcgtc ccagcctgca tgtcctcgtt cgtcgcgagg acgtcgatgc
    21541 ctcgcgcctc gacgggcggg tggtggtggt gatcgatgtg ttgttcgcga cctcgaccat
    21601 cgtgcatgcg ctcgcgagtg gcgcgtcgcg ggtctacccc gcgttcgacg ccgaggatgc
    21661 gcggcgcgca gcggttgccc gccccggcgc actcgtggcc ggcgaatacc tggcgaagac
    21721 cctgccggaa cacgccccgg ccaccccgct ggcgctggcg gcgcgcctgg agacgggtcg
    21781 cgaattggtc tacttcacca ccaacggcac ccgcgcgatc gccgcgtgcg agggcgcggc
    21841 ggccgtctat gccggcgcgc tgaccaacgc cgccgcactt gcggcgcaca tcgccgccac
    21901 ccatccacgc acgccggtgc tgctggtctg cgccggttcg gtcgggcgtt tgtgcctgga
    21961 ggatttcatc ggcgccggac acctggtgcg ggcgttgcgc gacaacggcg actacgcgct
    22021 caccgacgcc gccctggcgg ccgcgttcgc cgttggtggc gcggacatcg accacctcct
    22081 cacggcatcg cgggtggggc ggatgttgtc gcgcgcaggg atgggcgacg aaatcgcgca
    22141 tgccgcggcc tgcgattcca gcccggtggt gccggtcctg cgcgatggcg cgctggtcag
    22201 ggcggccgca tgagcgcgcc ttcgctgttc cccgatgtcg aaggcaatgc aacgcgcctg
    22261 caggcctggt tgcagggcca actggaaggc ttcggtgggg cgatggacat cgagcgcctg
    22321 cacggtgggc gctcgaatcc cggctggcgc ctgcgcacgg cgcatcgcga ttgcgtgctg
    22381 cgtgcgcgtc cgggtccacg cgccaacttg ttgccgtccg cgcacgcgat cgagcgcgaa
    22441 ttccgcgtgc aacgcgcgct ggccggcagc gcggtgccgg tggccgcgat gcatgcactc
    22501 tgcgaggacg aagcgatcat cggcgccggc ttctacgtga tggacttcgt cgacggcgac
    22561 atcctgcgcg agcaggcgct gcccggactg gaaccctcgc agcgcgccga gcgctacgac
    22621 gcgatgaacg ccggcatcgc tgcgctgcac ctgctcgatg tcgatgcact gggcctgcgt
    22681 gactacggcc gcagcggcca gtacttcgcc cgccagatcg accgctggac gcgccagtac
    22741 gtggctagcc gcgacggcga gatcgccgac atggacaagc tgatcgcgtg gctgccgctg
    22801 cacgtgccgc gcgaagaaga cgaaccggtc gcgctggtgc acggcgattt ccgcatcgac
    22861 aacatggtgt tcgcacgcgg cgagccccgc ctgcttgccg tgctcgattg ggagctgtcc
    22921 acgctgggtc atccgttggc cgatttcgcc taccacgtga tgagttggca tatcgcgccc
    22981 acgccactgt ccgggttggg tgggctggac ctcgatgcgc tcggcatccc cgatgagcag
    23041 gcctatgtgc aggcctacga gcgccggacc ggcatgaagg tgggcggaca atgggacttc
    23101 agcctggcct acaacctgtt ccggttggcc gcgatcctgc agggcgtcgg cgcgcgcgcg
    23161 cgcgccggca tggccaccga tcccgccgcg ctgcagttcg ctgcgcaggt gccgaccctc
    23221 gcagcgctcg gctggggctt tgcgcaacgc gccggcagca acgcatgacg tttccccgca
    23281 ttcccttcca cgccaaggtg cagacgccga catggctgta accacgacct cgcgcgacgg
    23341 catcgccgtc atcaccttcg acaacccgcc ggtcaacggc atgggcctgg ccgtgcgccg
    23401 cggcatcgcc gatgcgatcg ccgccgccaa tgccgacgac gcgatcgccg gcatcgtgat
    23461 caccggcggc ggcaaggtgt tttccggtgg cgccgacatc aaggaattca acacgccgcg
    23521 cgcggagcag gcgccaaacc tgcacgacgt gatcgccgcg gtggaagcca gccccaagcc
    23581 ggtctgcatc gccatcaacg gcaccgcgct gggcggtggg atggagttgt cgctgggtgc
    23641 gaactaccgc gtcgcccatg cgaaggcgga agttggcctg cccgaagtga agatcggcat
    23701 cctgccgggt gcgggtggca cccagcggct gccacgcgtg gtcgggctgg agaccgcatt
    23761 gaacatgatc gtgtccggtg cctcggtgcc ggccgcgaat ctcgcccgca ccgccctgtt
    23821 cgatgcggtg gtcgatggcg atgtggtgga cgccgcggtt gacctgctca agcagaagat
    23881 cgccggcggt ggcccacatc cgcgcgtgcg tgatcgcaag gtcgagcatg acgatgccga
    23941 ggcgttcatc gcctgggcgc gcatgggcgt ggcggcggcc tcgaaagact tcccggcacc
    24001 gttgcgttgc gtggatgcgg tagcggcgtc gctgaagccg ttcgatgccg gcatggcggt
    24061 cgagcgcgaa ggcttcaccg tgctgctgaa ctcgccggaa tcgaaggcgc tgcgacatgc
    24121 gttcttcggc gaacgcgccg ccggcaagat cgacgacatc ggtgcggaaa taccgccacg
    24181 cgcgatcgac aaggtgggcg tgatcggcgc cggcaccatg ggcg
//
LOCUS       DAOOCX010000028        24159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1768_length_24159_cov_9.047917, whole genome shotgun sequence.
ACCESSION   DAOOCX010000028 DAOOCX010000000
VERSION     DAOOCX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 24159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24159
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1768_length_24159_cov_9.047917"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="leuB"
                     /locus_tag="PK399_04285"
     CDS             <1..957
                     /gene="leuB"
                     /locus_tag="PK399_04285"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPW12372.1"
                     /translation="AAIDATGTPLPADTLDAAQAADAILLGAVGGPKWSDPNAKVRPE
                     QGLLAIRKALGLFANLRPVKPHVAGLGASPIKPHLLQGVDLVVVRELTGGIYFGRKQR
                     NADSASDLCEYSVVEIERVVRHAAQLARSRSGRLTSVDKANVLETSRLWRDVTTRVVR
                     DEFPDVTLEHQLVDSMAMHLIAKPRAYDVIVTENMFGDILTDEASMLAGSLGLLPSAS
                     LGEGRVGIYEPIHGSAPDIAGLGVANPYACILSAAMLLRHSLGLEGEAQCVEQAVSRA
                     LDAGHLTADLAGEGQRAIGTRAATEAVIEQLELPCQVMELRD"
     gene            1035..1916
                     /locus_tag="PK399_04290"
     CDS             1035..1916
                     /locus_tag="PK399_04290"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPW12373.1"
                     /translation="MPSSPMRLPAAHRAFRRTLAFLLLLLAAGLAVASDPGDLHVATY
                     NLRLDVASDGANAWPHRRDAVKALVRRHGFDVFGTQEALPGQLADLDALTEYARVGVG
                     RDDGKQAGEHSAIFYRRDRFELLAHGDFWFSQTPEVPSKGWDARCCNRLASWARLHDK
                     ASGKALTVVSVHFDHEGEVARRESAALLLRWLAGRDSGDALVALGDFNSGPETEQIRR
                     MQAVMGDARLRSEAPPYGPVGTFNGFAMGQPPGPRIDYIFVGPRIRVLDYAVLTDADG
                     QRYPSDHFPVTARLRVE"
     gene            complement(1923..2567)
                     /gene="leuD"
                     /locus_tag="PK399_04295"
     CDS             complement(1923..2567)
                     /gene="leuD"
                     /locus_tag="PK399_04295"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPW12374.1"
                     /translation="MKAFTTHTGLVAPLDRANVDTDQIIPKQFLKSIRRSGFGPNLFD
                     TWRYLDVGEPGQDHGLRPLNPDFVLNAPRYAGASVLLARENFGCGSSREHAPWALEEY
                     GFRAVIAPSFADIFYNNSFKNGLLPIVLAAHDVDTLFAQCEATEGYALTVDLATQTVT
                     RPDGVSHSFEVDEFRTHCLLHGLDDIGLTLQHADAIRAFEARHRAAQPWVFARG"
     gene            complement(2578..4005)
                     /gene="leuC"
                     /locus_tag="PK399_04300"
     CDS             complement(2578..4005)
                     /gene="leuC"
                     /locus_tag="PK399_04300"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPW12375.1"
                     /translation="MAGKTLYDKLWEMHEVVRRDDGSSLLYIDRHILHEVTSPQAFEG
                     LRLAGRKPWRIDANIATPDHNVPTTKIERDGGLQAIADPISRLQVQTLDENCDDFGIL
                     EFRMDDVRQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLA
                     TQCLVAKKMQNMQVRVEGALPFGVTAKDVVLAIIGKIGTAGGNGHALEFAGTAIRALS
                     MEGRMTICNMAIEAGARVGMVAVDQKTIDYVRGRPFAPKGADWDAAVAAWADLVSDDD
                     AQFDTVIELRAEDIKPQVSWGTSPEMVLAVDQRVPDPDREPDPTKKDSITRALKYMGL
                     QANQPITTIRLDRVFIGSCTNSRIEDLRAAAEVARGRKVAASVKQALVVPGSGLVKAQ
                     AEAEGLHTIFLEAGFEWREPGCSMCLAMNPDKLGDGEHCASTSNRNFEGRQGSGGRTH
                     LVSPAMAAAAAVAGHFVDVRELMQP"
     gene            4160..5035
                     /locus_tag="PK399_04305"
     CDS             4160..5035
                     /locus_tag="PK399_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPW12376.1"
                     /translation="MDWASLNAFVAVADHGGFSAAAAQLHLTQPAVSKRIALLEASLG
                     ARLFDRLGRQVLLTEAGRLLLPRAKQMLAEADAARRAIDDLDQDIGGRLSLATSHHIG
                     LHRLPHLLRRFSALHPRVALDLGFMDSEQAYARVLQGSAELALTTLGPTQPPLRSTPL
                     WDDPLHVVVAPDHPLAMRTKVSLADLAAHPAVLPDAVTFTHRIVAEAFARRGLALQLR
                     MTTNYLETIKMLAAVGLAWTVLPQTMLDAQVRVLPVTGVKLTRQLGYTIHGGRTLSRA
                     AQAFIDLLKADASPG"
     gene            complement(5282..8458)
                     /locus_tag="PK399_04310"
     CDS             complement(5282..8458)
                     /locus_tag="PK399_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW12377.1"
                     /translation="MRFNLSEWALRNRPIVLFAILMLGLLGAWSYKHLGQSEDPPFTF
                     KAMVVRTIWPGATAEEVSRQITDRIEKKLMETGQYEFIRAYSRPGESQIIFIAKDSLG
                     SKDIPPLWYQVRKKVGDIKATLPRDAVGPFFNDEFGDTFGNIYALTGDGFDYAVLKDY
                     AERLELELQRVPDVGKVDLLGLQDEKIFIELSNTKLASLGIPLTDVQEALDQQNAVVP
                     ASYFETAGERVQLRVSGRFDSVQSIREFPIRSGDRTFRLGEIATVTRGFSDPPAPRMR
                     FMGEDAIGLAVSMRDGGDILKLGKSLETTFARLQKTLPTGMELRKVSDQPLAVTRSVD
                     EFIKVLTEAVAIVLLVSFLSLGLRTGTVVALSIPLVLAMTFAAMHYFGIGLHKISLGA
                     LVLALGLLVDDAIIAVEMMVVKIEQGYDRIKAAAFAWESTAFPMLTGTLVTAAGFLPI
                     ATAASSTGEYTRSIFQVVTIALLISWVAAVVFIPYLGYQLLEEQGAEAAPKGRIGRGF
                     RRLRDAFAAGARRIPRVGEALSDHITAKHHHEAAHDPYRTPFYQRFRRLVAWCVKYRK
                     TVIAVTVVVFIASLALFRFVPQQFFPSSTRLELMVDMELAEGASLKATAAQAKKLEAM
                     LRKQQGVESYVAYVGTGSPRFYLPLDQQLPAANFAQFVLLTHDIESREAVRQWLIDTA
                     APAFPELQVRVTRLENGPPVGYPVQFRVSGEHIDRVQAIARQVADKVRANPHVTNVNL
                     DWNEPSKVVRLRIDQERARALGVSSAAVAKFLSGSLSGTSVSTYREGNEAIEILLRGP
                     GEERERLGMLASLAVPTASGKAVPLSQIATLEDGFEDGIVWHRNRLPTVTVRADIYGK
                     ELPASVMAQIGPTLDPIREALPSGYLLQLGGTVEDSARGQNSVNAGMPLFVLVVTTLL
                     MLQLRSFSRVLLVWLTAPLGLIGVSLFLLVLRVPFGFVAMLGTIALFGMIMRNSVILV
                     DQIEQDIAHGSAPWDAISDATVRRFRPIVLTALAAVLAMIPLSRSVFFGPMAVAIMGG
                     LIVATALTLLFLPALYAAWFRVKPPQAA"
     gene            complement(8465..9541)
                     /locus_tag="PK399_04315"
     CDS             complement(8465..9541)
                     /locus_tag="PK399_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW12378.1"
                     /translation="MRAVLGMALLAALAACGGQAPVAETPRPVLVVHPAAGADTAPAA
                     YAGDIRARNESPLSFRVGGNLVRRLAEVGDRVRQGQLLAELDPGDAALQASAAQAEMI
                     RIGGDLERYRKLVAQKLVSQSTFDAQQAAYRAARAQYELTRNQSAYTRLHAPRDGVIA
                     TRSAEAGQTVAAGQAIYTLAGDNGREVAIALPESRIRDVSVGQQVMVELWSAPGQLLQ
                     GRVREISPAADPQARTYAARVALEGEAANAVELGQSARVYMQQSGERAALRVPLSAVQ
                     RDAEGKPASLWVVDPASRRVRLAPVRLGAYAEGTVPLLGGVDANAWIVAAGGHLLRDG
                     QLVAPVDRDNRPVLAPSRNATAGK"
     gene            9643..10305
                     /locus_tag="PK399_04320"
     CDS             9643..10305
                     /locus_tag="PK399_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017167389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW12379.1"
                     /translation="MSSRTAKTAADATPKPAGPGRPKDPGKRAAILDAAKRMFVAHGF
                     DGVSMDQIASEAGVSKLTVYSHFGDKESLFAEAVRAHCEAGMPTSLFEADPAMPLRER
                     LTSIGNAFFAMIMTPEAIAGHRILCSPQVATSAMPAAFWEAGPQRVQQAFTELLERRI
                     AAGDLEIDDTERAAAQFFTLLKGEAHAKAVFGYCCGRRQETPEEHVAAVVELFLRAYA
                     RR"
     gene            10438..11091
                     /locus_tag="PK399_04325"
     CDS             10438..11091
                     /locus_tag="PK399_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HPW12380.1"
                     /translation="MNIDFARARETMVEQQVRPWDVLDARVLDTIATLPREAFVPQAV
                     RSLAYADTALPLAHGETMMKPVLEGRTLQSLLPQSQESVLEIGTGSGYLTACLAHLSR
                     EVVSLERHPDLADAARARLSAQGIANAEVITADALAWTTDRSFDAICVTGAVDRIPAR
                     FTDWLKPGGRLFVVRGRSPAMEAVLVHRDVNGLRTESLFETDLPYLAGAAPAPAFAL"
     gene            11130..12485
                     /locus_tag="PK399_04330"
     CDS             11130..12485
                     /locus_tag="PK399_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HPW12381.1"
                     /translation="MSRRPLALALVLALLPVAAQADDLLQSYTNARNSDPQYAAAESN
                     RAIAAERPVQARAVLLPQVNGSVGYDLANRGDTDGDGQSDGTSRSASWGLNAQQSIFD
                     YGNYTALRAAKALDRAGGFDLDAAGHSLITRTSAAYFNVLVQLETLSAAEAAESALKK
                     QFDFASKRLEVGLAPITDVHEARAQYDGARANTILARNAVQDAYQALVEITGTPVANL
                     KGLPNDFKPALPEARDVDGWVATALSENPSLKAQAADLEAAEHNVATARSGHYPTLGL
                     RASYGKAIPGLYDNPSFFGNNDANTSVGLTLSIPIFSGFATQSNVRSSLAQRDIARDQ
                     LEQQKRALERNTRNAYQALVAGISEVEARRLALVSAQSAYDASQVGLEVGTRTVLDVL
                     NNQRTLFSAQQAYAQSKYNFLQSRLLLEQAAGTLEISDVEDVNRLLTTDESITRGLGN
                     P"
     gene            complement(12496..13797)
                     /gene="waaA"
                     /locus_tag="PK399_04335"
     CDS             complement(12496..13797)
                     /gene="waaA"
                     /locus_tag="PK399_04335"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HPW12382.1"
                     /translation="MRADFTERVLRGLYSAALYVLVPITLYHLIWRGFRQRAYFHRWS
                     ERYANYPERGPSAPLWLHAVSVGEVNAAAPLVDRLRQLRPDLRLVITTITPTGSERVR
                     ALWGDAVTHVYLPYDLPGAVGRFLDHFRPALALIVETELWPNLLFGCRDRGIPTHILN
                     ARLSARSLRGYRVLAPLIRRALRTVRLVAAQSRDDARRFLMLGAQPERVQVSGNLKFD
                     MAPVDAAAFAADFATHRGTAGPVWIAASTHEDEEAAVLDAHRLLRRSQPDALLLWAPR
                     HPERFRAVAERAQAAGFGVGTRSTDTWPGPDAEVFVVDTLGELVRFYACAAVAFVGGS
                     LQPVGGHNLLEPAATGTAILSGPHLHNFADIARRLREAEAMRVVDDAGALAHALQALF
                     ADEPARLRLAANASRLLEDGRGALARTVTLIEPALGAAGSG"
     gene            13842..14759
                     /gene="lpxL"
                     /locus_tag="PK399_04340"
     CDS             13842..14759
                     /gene="lpxL"
                     /locus_tag="PK399_04340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HPW12383.1"
                     /translation="MNDAVRMPPPLGPRHWPAWLGIGIAALLARVPWSLQRVLGRGFG
                     RLLMRVFGSRRRVAARNLALCFPELDADAQAALLRRSFEELGIGLFEFARAWWGSVEP
                     MRGKVAVEGLEHLEAARAGGRGVIIISGHFVTLELAARLMCDHAPLAGMYRPHDGAAM
                     EWAVLRGRLRYATAMFTREELRPALRHLKQGGLLWFAPDQDTRRGDSVFVPFFGRPAY
                     SLTSTHQLARLSGAAVLGFAHVRHDDGGYTLRLTPAFEDFPSSDATADTARVMAAIET
                     MIRAAPAQYLWIHRRFKRQPDQPDNALYR"
     gene            complement(14750..15631)
                     /locus_tag="PK399_04345"
     CDS             complement(14750..15631)
                     /locus_tag="PK399_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW12384.1"
                     /translation="MRRCWHGRAFAALGRWVAGRSPARAQALARRLVRLPWINAKRRR
                     IAATNIALCFPHLDDAAREALLADTLVSNTTGVLETLRGWFATPAQLAGQAQIVGIEH
                     LHAALAEGRGVVVVGAHYDGIELAMRHVADASGQPMPILVRRNNDPCIEAAIAEARCS
                     YAGATYDKKDIAGFAAAVRAGQGVFYVPDQNAARRTVFVPFLGVQAATLGAIGGVLRR
                     AGGRVLLMWARRDADGRQHIDLQPAWEGWPAVDDADTAARYMAWIGERLQAAPEQYLW
                     VHRRFKTRPPGEPPVYR"
     gene            complement(15628..16392)
                     /locus_tag="PK399_04350"
     CDS             complement(15628..16392)
                     /locus_tag="PK399_04350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW12385.1"
                     /translation="MGVRLSAVVTTLDNAATLEACLASLRFCDELVLLDSGSTDATLD
                     IARRHGARIASQPFAGYGPQKQAAIDLAEGEWILLLDADESLTDDGRATLLRELDAPR
                     ADGYRLPRREWLFWRWPHPATRPNWQLRLFRKARGRMNAVPVHAAPVVEGRVIDLDAP
                     FRHYGEPRLADRVEKVNRYSSGLVEHKRERRVRFIGLRLLVYPPLVFLKLYLGKRYVL
                     NGWAGYFAARTQAFYAFLKYAKLLEATRSPGPGPRA"
     gene            complement(16394..17689)
                     /locus_tag="PK399_04355"
     CDS             complement(16394..17689)
                     /locus_tag="PK399_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPW12386.1"
                     /translation="MNDATQAVHGWRWAPAWVLAFVALWPAPGYAEGVLALGALAGLL
                     RLVASRFRGGNALLSGPAWALTSVLFFAYWLPEAVSAIDAMDRGRALREALVDLRYLP
                     FLWIVAAAVADDAGRRRTFGGLAIIVGAWTLDALIEAATGASPLFSGIDMVKHAISGH
                     GMCTAEQVAAADRLAGVLGPCNLKLGIVLASLSPFALDAMRRRFGRIGWLVAAVGIGI
                     VVLLAGSRASWLTYGLVLLWTGWRTLGRRKLLMVFALGALALVAATLVVPQVRERIER
                     TTHVLTADSKGVDTALSGRSRIWSAAVCMAREHPVNGVGARGFRQAFAACDPQPGVIA
                     AWGEGAALHAHQLVLEILSETGGIGLLLWLAGAALAWRAWRYADPVARDRARPAMLAL
                     AVTVFPLNTHLASYSTFWGGVLLMLSALYAGSLLGREQD"
     gene            complement(17686..18798)
                     /locus_tag="PK399_04360"
     CDS             complement(17686..18798)
                     /locus_tag="PK399_04360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004426389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW12387.1"
                     /translation="MRRLTVVQLLPALDAGGVERSTLEIAEALVDAGHRAIVVSAGGR
                     LLAQLQAIGAEHLTLDIGRKSPLVLRHVRALRALLREAGADIVHARSRLPAWLGWWAL
                     RGTHGKRPRFITTAHGLNSPSRYSAIMARGERVVCVSRTVRDHLLAHYPATDPSRLVV
                     IPRGIDPAAFPRMPHPDRAARARIAGRHPQLEGEGPLLLLPGRGTRLKGHTDAIALLA
                     ALRGAGTDARLWMPGMVQAGREAYVRELVEMTRRASIEDAVAMTEPTADMPTAYAASD
                     LVLQLSRKPEAFGRTVIEALSIGRPVLGWDHGGVGELLRELQPEGAVLPFDATALREC
                     AQALLANPPVPPATMPYTLRAMQEATLAQYHALVQA"
     gene            complement(18814..19008)
                     /locus_tag="PK399_04365"
     CDS             complement(18814..19008)
                     /locus_tag="PK399_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HPW12388.1"
                     /translation="MTTPASSPANAQQRYEVRRADLPLSCPQPSMALWNSHPRVYLPI
                     EEEGGNSQCPYCGATYVLVD"
     gene            complement(19120..19833)
                     /locus_tag="PK399_04370"
     CDS             complement(19120..19833)
                     /locus_tag="PK399_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPW12389.1"
                     /translation="MIIERPSSARGEVDLGWLRSRHTFSFGHYHDPAWMGFGPLRVIN
                     DDTVAPGGGFSPHRHANMEIISVVLQGALAHRDSTGTVGMIEAGDVQWMSAGHGIEHS
                     EYNGSGSGAAPVHFLQIWIQPDRLNAQPAYAQQRFEPADRRGRWATLASPDGVDGSIA
                     IRQQATLRAAGLQPGDEVSASRDPTRLYWLHVATGEVAVGERVLKAGDALGFVQEADA
                     LVIRGIAAASDVLLFDLPA"
     gene            complement(19841..20431)
                     /locus_tag="PK399_04375"
     CDS             complement(19841..20431)
                     /locus_tag="PK399_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPW12390.1"
                     /translation="MFKKLLLTAALVAASANAFAAGVTYKLDPTHTNVLASWNHFGYS
                     NPYANFGDVEGTLVYDAADVSKSSVQVTLPLSGLNGFSDKFNAHLRSADIFDIATFPT
                     ATFKSTKVESAGNGKLKITGDLTIKGITKSVVLDATLNKASEHPMKKVPAIGFDATAT
                     IKRSDFGVGLFAPMVGDEVTLRITTEATAPKADAAK"
     gene            complement(20460..>20923)
                     /locus_tag="PK399_04380"
     CDS             complement(20460..>20923)
                     /locus_tag="PK399_04380"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HPW12391.1"
                     /translation="TEANTTPARIVFVKSDEAKAKLGPALSEGNFKKTMAAPCVAIVG
                     YDMRFFDKLPVLFPHVDAKPWFEHRDEANLAWVANRNSSLQTAYLILAARALGLDCGP
                     MTGFDNAKVDEAFFAGTSIRTSILVNLGHGDPASIFPRSPRLGFDEACQIL"
     assembly_gap    20924..21023
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21024..21205)
                     /locus_tag="PK399_04385"
     CDS             complement(<21024..21205)
                     /locus_tag="PK399_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPW12392.1"
                     /translation="MTQPLPDAALDQLFRTARTYNAFTGEVSDDTLQQLYGLLKFGPT
                     EANTTPARIVFVKSDEA"
     gene            21319..22269
                     /locus_tag="PK399_04390"
     CDS             21319..22269
                     /locus_tag="PK399_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HPW12393.1"
                     /translation="MTSTVALHDDRAGNTRQAMALARALDADAGECLLQPRAPWRWLA
                     PRVLPGATHGFGGEFAALIARPPAIAVGCGRQAALATRLLRARGSRAVQILDPRIDAR
                     HWDLVVAPEHDGLRADNVIQMLGSLHPVDDLWLAQARRDAAHLAALPSPQTVLLVGGP
                     TRHAALDDAGFAALLARLSAHAHHESGSFSVVASRRTPDAWRASLSNVSADIPGIRWR
                     DERDGPNPYAGLLAHANRIACTPDSVNMLSEAAATLAPVFVWSADIATGRLRAFLDAL
                     RSRGRVRDLDDALAPFAVDPLRETARVASEVRRRLALPIA"
     gene            complement(22255..>24159)
                     /gene="glnE"
                     /locus_tag="PK399_04395"
     CDS             complement(22255..>24159)
                     /gene="glnE"
                     /locus_tag="PK399_04395"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HPW12394.1"
                     /translation="ARKDMADDIKRGPGGIREIEFLVQALQLIRGGREPALRDRRLLP
                     AMRALVEAGHVDAATGAMLLEAYRFLRTLENRLQMLGDAQTHALPAGALLRARIADGL
                     GYAHWQALLEALAVQRERVSAEFDALLAARRAASGDSDLAAYWRGLPDAGDGAVLAAA
                     GFEAGEDLHRSLADFARVPAVRTLSDATRARLDRVLPALLQAAVASPHPDAVLRHLLP
                     LLHMLLRRASYLALLDEQPVALHRLVDALARSALLGERLAAHPLLLDELLDVRIGGEL
                     PGREAMRQACTAALGDGDADTEAALDALNEIRQALSFRIALALLDRRIEAQECARRLA
                     WLADAVVDCILKLALAGMHAAHGGIDGARFAVLGYGSLGGEELGFGSDLDLVFLYDAV
                     EGATSDGARPLDAARWFARLGQKIVALLETRTGAGRLFEIDVRLRPDGAKGLLVSSLA
                     SFSDYQRERAWTWEHQALVRARAVAGDVALIDAFEGVRGDVLSRGRPVDGLRDDVSAM
                     RRRMRAELDRSDAALFDLKQGDGGLVDLEFLLQFLLLRDSRTHARLRTPRASPAILDA
                     LHDAGLLDANTRAALQVAHATLLDAGLRCTLDRRPRRVALDERITLAREAIRDAVRAQ
                     GLQFEASGDR"
BASE COUNT         3866 a         8537 c         8043 g         3613 t
ORIGIN      
        1 gccgccatcg acgccaccgg caccccgctg ccggccgaca ccctggatgc tgcacaagcc
       61 gcggacgcaa tcctccttgg cgccgtgggt ggtccgaagt ggtcggaccc gaacgcgaag
      121 gtgcgtccgg aacagggcct gctggcgatc cgcaaggcac tcggcctgtt cgccaacctg
      181 cgcccggtca agccgcacgt ggccggcctg ggcgcctcgc cgatcaagcc gcacctgctg
      241 cagggcgtgg acctggtggt ggtgcgggaa ctgaccggcg gcatctactt cggccgcaag
      301 cagcgcaacg cggacagcgc cagcgacctg tgcgaataca gcgtcgtcga gatcgagcgc
      361 gtcgttcgcc atgccgcgca actcgcgcgc agccgcagcg gccggctgac ctcggtcgac
      421 aaggccaacg tgctggaaac ctcgcggctc tggcgcgacg tcaccacccg cgtggtccgc
      481 gacgaattcc ccgacgtgac gctggaacac cagcttgtgg attcgatggc gatgcacctg
      541 atcgccaagc cgcgcgccta cgacgtgatc gtcaccgaga acatgttcgg cgacatcctc
      601 accgacgagg cctcgatgct ggccggctcg ctgggcctgc tgccgagcgc atcgctgggc
      661 gaagggcgcg tcggcatcta cgaaccaatc catggttccg cacccgacat cgccgggctg
      721 ggtgtcgcga atccgtatgc gtgcatcctc agcgcggcga tgttgctgcg ccactcgctg
      781 ggcctggaag gcgaagcgca atgcgtcgag caggcggtgt cgcgcgcact ggatgccggc
      841 cacctcaccg ccgacctggc cggtgaaggc cagcgcgcca tcggcacccg cgccgccacc
      901 gaagcggtca tcgaacaact ggaactgccc tgccaggtga tggaactgcg cgactgaggc
      961 agacgcgcag agggaggtgc acgttggcac ccgtttgctg tccaatggtg gccctgccgc
     1021 ctccagacgc ttccatgcct agctccccca tgcgcctgcc tgctgcgcat cgagcatttc
     1081 ggcgcacgct tgcattcctc ctgctcttgc tggccgccgg cctggcggtg gccagcgatc
     1141 ccggcgacct gcacgtcgcc acctacaacc tgcggctgga cgtggccagc gacggcgcca
     1201 atgcctggcc acaccggcgc gacgcagtga aggcgctggt gcgccgccac ggcttcgacg
     1261 tgttcggcac ccaggaagcg ctgccggggc agcttgccga cctcgacgcg ctgacggagt
     1321 acgcccgcgt cggtgtcggc cgcgacgacg gcaagcaggc cggcgagcac tcggcgatct
     1381 tctaccgccg agaccgcttc gagctgctgg cgcatggtga cttctggttc agccagactc
     1441 cggaggtccc gtcgaaaggc tgggacgcac gctgctgcaa ccgactggca agctgggcgc
     1501 gcctgcacga caaggccagc ggcaaggccc tcaccgtggt gtcggtgcac ttcgaccacg
     1561 agggcgaagt ggcccgccgc gaatcggccg cactgctgct gcgctggctg gccgggcgag
     1621 actcgggcga tgcactggtc gcgctgggcg acttcaactc cggcccggag accgagcaga
     1681 tccgccgcat gcaggcggtg atgggcgatg cgcgcctgcg ctcggaagcg ccgccatacg
     1741 gccccgtggg caccttcaat gggttcgcga tggggcaacc tcccggcccg cgcatcgatt
     1801 acatcttcgt cggcccgcgc atccgcgtgc tcgactacgc ggtgctgacc gacgccgacg
     1861 gccagcgcta cccgtccgac cacttcccgg tcacggcgcg gctgcgcgtg gaatagccgc
     1921 gctcaaccgc gtgcgaacac ccacggttgc gccgcgcgat ggcgcgcctc gaaggcgcgg
     1981 atggcatcgg cgtgctgcag ggtcaggccg atatcgtcca ggccatgcag caggcaatgc
     2041 gtgcggaatt cgtcgacctc gaaggaatgc gagacgccat ccggccgggt cacggtctgc
     2101 gtggcgaggt ccacggtcag cgcatagcct tcggtcgcct cgcactgcgc gaacaaggta
     2161 tcgacgtcat gcgcggcgag cacgatcggc agcagcccgt tcttgaaact gttgttgtaa
     2221 aaaatatcgg cgaagctggg cgcgatcacc gcgcggaaac cgtattcctc cagcgcccac
     2281 ggcgcatgct cgcgcgagga gccgcagccg aagttctcgc gcgccagcaa cacgctggcg
     2341 ccggcatagc gcggggcatt gagcacgaaa tcggggttga gcggacgcag gccatggtcc
     2401 tgcccgggct cgccgacatc caggtaacgc cacgtgtcga acaggttcgg gccgaagccg
     2461 ctgcgcctga tcgacttcag gaattgcttc gggatgatct ggtcggtatc gacattcgcg
     2521 cgatccagcg gcgcgaccag tccggtgtgg gtggtgaagg ctttcatcga cgtgtcctca
     2581 aggctgcatc agttcgcgca catcgacgaa gtgcccggcc accgcagctg cagcggccat
     2641 cgccgggctg accaggtggg tgcgaccacc gctgccctgg cggccctcga aattgcggtt
     2701 ggacgtggat gcgcagtgct cgccatcgcc cagcttgtcg ggattcatcg ccaggcacat
     2761 cgagcaaccc ggctcgcgcc actcgaagcc ggcctccagg aaaatcgtgt gcagtccctc
     2821 ggcttccgcc tgcgccttga ccaggccgga gcccggcacc accagcgcct gcttcaccga
     2881 tgcggcgacc ttgcggcccc ttgcgacctc ggcggcggcg cgaaggtctt cgatccgcga
     2941 attcgtgcag gagccgatga acacgcggtc gagcctgatc gtggtgatcg gctggttggc
     3001 ctgcaggccc atgtacttca gcgcgcgagt gatcgagtcc tttttggtcg gatccggttc
     3061 gcgatcggga tcgggcacgc gttggtcaac cgccagcacc atctccggcg acgtccccca
     3121 gctgacctgc ggcttgatgt cctcggcgcg cagttcgatg acggtgtcga actgcgcatc
     3181 gtcgtcggag accaggtcgg cccacgccgc caccgccgcg tcccagtccg cccccttcgg
     3241 tgcgaacgga cgcccgcgca cgtagtcgat ggtcttctgg tccaccgcga ccatgccgac
     3301 gcgcgcgccg gcctcgatcg ccatgttgca gatcgtcatc cgcccttcca tcgacagcgc
     3361 gcggatcgcg gtgccggcga attccagcgc atggccattg ccaccggcag taccgatctt
     3421 gccgatgatc gccagcacca catccttggc ggtgacgccg aacggcaacg cgccctcgac
     3481 ccgcacctgc atgttctgca tcttcttggc gaccaggcat tgcgtggcca gcacatgctc
     3541 gacctccgac gttccgatgc cgtgcgccag cgcgccgaac gcgccatgcg tggacgtgtg
     3601 cgaatcgccg cagaccacgg tcatgcccgg cagcgtggcg ccctgctccg ggccgaccac
     3661 gtggacgatg ccctggcgca cgtcgtccat cctgaattcg aggatgccga agtcgtcgca
     3721 gttctcgtcc agcgtctgta cctgcagccg cgagatggga tccgcgatcg cctgcaggcc
     3781 accgtcgcgc tcgatcttcg tcgtcggcac gttgtggtcg ggcgtggcga tgttggcgtc
     3841 gatccgccac ggcttgcggc cggccaggcg caagccctcg aacgcctgcg gcgaggtcac
     3901 ctcgtggagg atgtggcggt cgatgtagag cagcgacgat ccatcatcgc ggcgcacgac
     3961 ttcgtgcatc tcccacaatt tgtcgtacag ggtcttgccg gccatgcgct gcctcggtca
     4021 ggtctggcgg caggcttgca tccaacgtcc acccgccggc tggaggctca tcctgccgac
     4081 ttgccatcga taactccaat ggctattttt catcctttcg attcccgaat ggaatcctaa
     4141 taaggcatcg ggggatcgcg tggactgggc cagcctcaat gccttcgtcg cggtcgccga
     4201 ccacggcggc ttctccgccg cggccgcgca actgcacctg acccagccag cggtgagcaa
     4261 gcgcatcgcg ctgctggagg cttcgctggg cgcgcgcctg ttcgaccgcc tcggccgcca
     4321 ggtgctgctc accgaagccg gccgcctgct gctgccacgc gcgaaacaga tgctcgccga
     4381 agccgacgcc gctcgccgcg cgatcgatga cctcgaccag gacatcggtg gccgcctcag
     4441 cctcgccacc agccaccaca tcggcctgca tcggcttccg cacctgctgc gccgtttcag
     4501 cgcgttgcac ccgcgggtag cgctggacct gggtttcatg gattccgaac aggcgtatgc
     4561 gcgggtcctg cagggcagcg ccgaactcgc cctgaccacg ctcggcccaa cgcagccacc
     4621 gttgcgttcc acgccactct gggacgaccc gctgcatgtg gtggtcgcgc ccgatcatcc
     4681 actggcgatg cgcacgaaag tgagccttgc cgatctcgcc gcgcatcccg cggtgttgcc
     4741 ggatgcggtg acgttcaccc accgcatcgt cgccgaggcc tttgcccggc gcggcctcgc
     4801 cttgcagctg cggatgacca ccaactacct ggagaccatc aagatgctgg cggccgtcgg
     4861 cctcgcctgg accgtgctgc cgcagaccat gctcgatgcg caggtgcgcg tgctgcccgt
     4921 gaccggcgtg aagctcacac gccaactcgg ctacacgatc catggcgggc gcaccctgtc
     4981 gcgcgcggcg caggcgttca tcgacttgct gaaggcggat gcctctcccg gataaacgat
     5041 caatccgaag cgcgtcggtg cggtgggtat cggcgcgcaa tcaaggagga ggcgtcggcc
     5101 caatcaactt ccaaacgact tgacgcttca agccgacgcc gggggacatg cgcgccgata
     5161 cccaccgcac cggcgcgcac gcgcagagct cccctcttcc ggcgccacgc gccaccctct
     5221 cccacgagga gagagggtta cctcaccggc aacccttgcg ccggcgcaac tcaggcaggg
     5281 gtcaagccgc ttgcggcggc ttcacccgga accatgccgc gtacaacgcc ggcaggaaca
     5341 gcagcgtcag cgcagtcgcc acgatcaggc cacccatgat cgccaccgcc atcggcccga
     5401 agaacaccga gcgcgacagc gggatcatcg ccagcactgc ggccagcgcg gtcagcacga
     5461 tcggccggaa gcggcgcacg gtggcgtcgc tgattgcatc ccaaggcgca ctgccatggg
     5521 cgatgtcctg ctcgatctga tccaccagga tcaccgagtt gcgcatgatc atgccgaaca
     5581 gcgcgatggt gccgagcatc gccacgaaac cgaacggcac ccgcagcacc agcaggaaca
     5641 ggctcacgcc gatcagtcca agcggcgcgg tcagccacac cagcagcacg cgcgagaagc
     5701 tgcgcagctg gagcatcagc agggtggtca ccaccagcac gaacagcggc atgcccgcgt
     5761 tcaccgagtt ctggccgcga gcggaatcct ccaccgtgcc gccgagctgc agcaggtacc
     5821 cggacggcaa tgcctcgcgg atcggatcca gcgtgggccc gatctgcgcc atcacgctgg
     5881 ccggcaactc cttgccatag atgtccgcgc gcacggtgac ggtcggcagg cggttgcgat
     5941 gccagacgat gccgtcctcg aagccgtctt ccagggtcgc gatctggctg agcggcaccg
     6001 ccttgccgct cgccgtcggc accgccagcg aggccagcat gccgaggcgt tcgcgttcct
     6061 cgcccgggcc gcgcagcagg atctcgatcg cctcgttgcc ttcgcgatag gtgctgaccg
     6121 aggtgccgga cagtgagccc gacaggaact tcgccaccgc cgccgagctg acgcccagcg
     6181 cgcgcgcgcg ctcctggtcg atccgcaggc gcacgacctt gctcggttcg ttccagtcca
     6241 ggttgacgtt ggtcacgtgc ggattggcgc gcaccttgtc ggccacctgg cgggcgatcg
     6301 cctgcacgcg gtcgatgtgc tcgccggaga cgcggaattg caccggatag ccgaccggcg
     6361 ggccgttctc caggcgggtg acgcgcacct gcaattccgg gaacgccggc gcggcggtat
     6421 cgatcagcca ctggcgtacc gcttcgcgcg attcgatgtc gtgggtgagc aggacgaact
     6481 gggcgaagtt cgccgccggc agttgctggt ccagcggcag gtagaaccgc ggcgaaccgg
     6541 tgcccacgta ggcgacgtag ctttccacgc cctgctgctt ccgcagcatc gcttccagct
     6601 tcttcgcctg cgccgcggtg gccttgagcg acgcgccttc ggccagttcc atgtcgacca
     6661 tgagttccag ccgcgtggac gacgggaaga actgctgcgg cacgaagcgg aacaacgcca
     6721 gcgaggcgat gaacaccacc accgtcaccg cgatcacggt cttgcggtac ttcacgcacc
     6781 acgcgaccag ccggcgaaag cgctggtaga acggcgtcct gtacggatcg tgcgcggctt
     6841 cgtggtggtg cttggcggtg atgtggtcgg acagcgcctc gcccacgcgc gggatgcgtc
     6901 gcgcaccggc agcgaacgca tcgcgcaggc gacggaatcc acggccaatg cgtcccttcg
     6961 gcgcggcttc ggccccctgt tcttccagca gctggtaacc caggtaggga atgaacacca
     7021 ccgccgcgac ccacgagatc agcagggcga tggtcaccac ctggaagatg gagcgcgtgt
     7081 attcgccggt gctcgacgcc gcggtggcga tcggcaggaa gcctgccgcg gtcaccaggg
     7141 tgccggtgag catcgggaac gcagtgcttt cccacgcaaa cgcagcggcc ttgatgcggt
     7201 cgtagccctg ctcgatcttc accaccatca tctcgaccgc gatgatcgcg tcgtccacca
     7261 gcaggcccag cgccagcacc agcgcgccga gcgagatctt gtgcaggccg atgccgaagt
     7321 aatgcatcgc ggcgaaggtc atcgccagca ccagcgggat cgacagcgcg accaccgtgc
     7381 cggtgcgcag gcccagcgac aggaagctga ccagcagcac gatcgccacg gcttctgtca
     7441 gcaccttgat gaattcgtcg accgagcgcg tcaccgccaa cggctggtcg gaaaccttgc
     7501 gcagctccat gccggtcggc agggttttct gcaggcgcgc gaaggtggtt tcaagcgact
     7561 tgccgagttt caggatgtcg ccgccgtcgc gcatcgaaac ggccaggccg atcgcgtcct
     7621 cgcccatgaa gcgcatgcgc ggcgcgggcg ggtcggagaa gccgcgggtg accgtggcga
     7681 tctcgcccag gcggaaggtg cggtcgccgc tgcggatcgg gaattcgcgg atcgactgca
     7741 ccgaatcgaa gcgtccgctg acgcgcagtt gcacgcgctc gccggcggtc tcgaaataac
     7801 tcgccggcac caccgcgttc tgctggtcca gcgcctcctg cacgtcggtc agcgggatac
     7861 cgagcgacgc gagcttggtg ttcgacaact cgatgaagat cttctcgtcc tgcaggccga
     7921 gcaggtcgac cttgccgaca tcgggcaccc gttgcagttc cagttccagc cgttcggcgt
     7981 aatccttcag caccgcgtag tcgaagccat cgccggtcaa cgcatagatg tttccgaagg
     8041 tgtcgccgaa ttcgtcgttg aagaacgggc cgaccgcatc gcgcggcagg gtcgccttga
     8101 tgtcgccgac cttcttgcgc acctggtacc agagcggcgg gatgtccttg ctgcccagcg
     8161 aatccttggc gatgaagatg atctgcgact cgcccggccg cgagtacgcg cggatgaact
     8221 cgtactggcc ggtctccatc agtttctttt cgatgcggtc ggtgatctgc cgcgagactt
     8281 cctcggcggt cgcgcccggc cagatcgtgc gcaccaccat cgccttgaag gtgaacggcg
     8341 gatcctcgga ctggcccagg tgcttgtatg accacgcgcc gagcaggccc agcatcagga
     8401 tcgcgaagag cacgatgggg cggttgcgca gcgcccattc ggagaggttg aagcgcatgg
     8461 cggcttattt cccggcagtc gcgttgcggg agggtgcaag caccgggcgg ttgtcgcgat
     8521 ccaccggcgc caccagttgc ccgtcgcgca gcagatggcc gcctgcggcc acgatccacg
     8581 cattcgcatc cacgccgccc agcaacggca ccgtgccttc ggcataggca ccgagccgga
     8641 ccggggcgag gcgcacgcgt cgcgaggccg ggtcgaccac ccacagcgat gccggcttgc
     8701 cctccgcgtc gcgttgcacc gccgacagcg gcacccgcag cgcggcgcgc tcgccggact
     8761 gctgcatgta aacgcgggcg ctctggccca actcgaccgc attcgcggct tcaccttcca
     8821 acgccacccg cgcagcgtag gtgcgggcct gcggatcggc agccggcgag atctcgcgca
     8881 cgcggccttg caacagctgg ccgggggcgc tccacaactc caccatcacc tgctggccca
     8941 cgctgacgtc gcggatgcgc gattccggca gcgcgatcgc gacctcacgc ccgttgtcgc
     9001 ctgccagcgt gtagatcgcc tgccccgcgg cgacggtctg cccggcttcg gcagagcgcg
     9061 tggcgatcac gccatcgcgc ggtgcatgca ggcgcgtgta ggccgactgg ttgcgggtca
     9121 gttcgtactg cgcgcgcgcg gcgcgatacg cggcctgttg ggcatcgaag gtcgactggc
     9181 tgaccagttt ctgcgccacc agcttgcggt agcgctccag gtcgccgccg atgcggatca
     9241 tttccgcctg cgcggcgctg gcctgcaggg cggcgtcgcc cggatcgagc tcggccagca
     9301 actgcccctg gcggacgcgg tcgccgacct cggcgaggcg tctcaccagg ttgccaccga
     9361 cccggaacga gagcgggctt tcattgcgtg cgcggatgtc gccggcatac gccgccggcg
     9421 cggtgtcggc acccgcggcg ggatgcacca ccagcaccgg gcgcggcgtt tccgccacgg
     9481 gcgcttgccc gccgcaggcg gcgagcgccg ccagcaatgc cattcccagc accgcacgca
     9541 tgttgcctgc gcccttcccc atgacccact ccgcggctcg tgcctttatt ggactggtcg
     9601 gtagagtata aatatcgaac cgaatagtct agtattccaa gcatgtcatc acgtaccgcg
     9661 aaaaccgccg cagacgccac ccccaagccc gccggtcccg gccggccgaa agaccccggc
     9721 aagcgtgccg ccatcctcga tgccgccaag cgcatgttcg tcgcgcatgg cttcgatggc
     9781 gtcagcatgg accagatcgc cagcgaggcc ggcgtctcca aactcaccgt ctacagccac
     9841 ttcggcgaca aggagagcct gttcgcggag gccgtgcgcg cgcactgcga ggccggcatg
     9901 cccaccagcc tgttcgaggc cgatcccgcg atgccgctgc gcgaacgcct gacctcgatc
     9961 ggcaatgcct tcttcgcgat gatcatgacc cccgaggcga tcgccgggca ccgcatcctt
    10021 tgctccccgc aggtcgccac aagcgccatg ccggcagcgt tctgggaagc cggcccgcag
    10081 cgcgtgcagc aggccttcac cgaattgctg gagcggcgca tcgccgctgg cgacctggag
    10141 atcgacgaca ccgagcgcgc cgcagcgcag ttcttcaccc tgctcaaggg cgaggcgcat
    10201 gcgaaggcgg tgttcggcta ttgctgcggc cgccgccagg agacgccgga ggagcacgtc
    10261 gccgcggtgg tggagctgtt cctgcgcgcg tatgcccgtc gttgaaccga cagcggctgc
    10321 gccgttggtg ccggtgactt ccggcactgc gtgccggtgt cctagcacag ccacactgcc
    10381 aaccatgaaa tgcgccgccg ttacaatggc cggctcgacg ccacggactt tccccgcatg
    10441 aacatcgact tcgcccgcgc ccgcgaaacc atggtggaac aacaggttcg cccgtgggac
    10501 gtgctggacg cccgggtcct cgacaccatc gccacgctgc cgcgcgaggc cttcgtgccg
    10561 caggccgtgc gcagcctggc ctatgccgac accgcgctgc cactggcgca cggcgagacc
    10621 atgatgaagc cggtgctgga aggccgcacc ctgcaatcgt tgctgccgca atcgcaggag
    10681 tcggtcctcg agatcggcac tggcagcggt tacctcactg cctgcctggc gcatctctcc
    10741 cgcgaagtcg tcagcctgga acggcacccc gacctggccg acgccgcccg cgcgcgcttg
    10801 tccgcgcagg gcatcgccaa tgccgaggtg atcaccgccg acgccttggc ctggaccacc
    10861 gaccgcagtt tcgatgcgat ctgcgtcacc ggggcggtgg accgcatccc ggcacgcttc
    10921 accgactggc tgaagccggg cggacggctg ttcgtggtgc gcggtcgctc gccggcgatg
    10981 gaagcggtgc tggtgcatcg cgacgtcaac ggactgcgca ccgagtcgtt gttcgagacc
    11041 gacctgccgt acttggccgg tgccgcaccg gcacccgcct tcgcactctg aagcccctct
    11101 cccaggacat cccgcaagga tctgccccta tgtcgcgtcg cccgcttgcc ctcgcccttg
    11161 tccttgccct gctgcccgtc gcggcgcagg cggacgacct gctgcagagc tacaccaatg
    11221 cacgcaacag cgacccgcaa tacgcggccg ccgaatccaa ccgcgccatc gccgccgagc
    11281 ggccggtgca ggcgcgggcg gtgctgctgc cgcaggtgaa cggctcggtc ggctacgacc
    11341 tggccaatcg cggcgacacc gatggcgacg gccagagcga tggcacctcg cgcagcgcat
    11401 cgtggggcct gaacgcgcag caatcgatct tcgattacgg caactacacc gcgctgcgcg
    11461 ccgcgaaggc actggatcgt gccggcggct tcgacctgga tgccgcgggc cacagcctga
    11521 tcacccgcac gtccgccgcc tatttcaacg tgctcgtgca actggaaacg ctgtcggccg
    11581 ccgaggccgc cgaatccgcg ctgaagaagc agttcgactt cgccagcaag cggctggaag
    11641 tgggcctggc gccgatcacc gacgtgcacg aagcgcgcgc gcagtacgac ggcgcccgcg
    11701 ccaacaccat cctggcccgc aatgcggtgc aggacgccta ccaggcgctg gtcgagatca
    11761 ccggcacgcc ggtggccaac ctcaagggac tgccgaacga cttcaagccc gcgctgccgg
    11821 aagcacgtga tgtcgatggc tgggtcgcca ccgccctgtc cgagaatcct tcgctgaagg
    11881 cgcaggccgc cgatttggaa gccgcggaac acaacgtcgc caccgcgcgt tcgggccact
    11941 atccgacgct tggcctgcgt gcctcgtacg gcaaggcgat ccccggcctg tacgacaacc
    12001 cgagcttctt cggcaacaac gacgccaaca ccagtgtcgg cctgaccctg agcatcccga
    12061 tcttctcggg cttcgccacc cagtccaacg tgcgcagctc gctggcccag cgcgacatcg
    12121 cccgcgacca gctggagcag cagaagcgtg cgctcgagcg caacacccgc aacgcctacc
    12181 aggcgctcgt cgccggcatc agcgaagtgg aagcgcgccg cctggcgctg gtctccgcgc
    12241 agagcgcgta cgacgcctcg caggtcggcc tggaagtcgg tacccgcacc gtgctcgacg
    12301 tgctgaacaa ccagcgcacc ctgttctcgg cgcagcaggc ctatgcccag tccaagtaca
    12361 acttcctgca gagccgcctg ctgctggaac aggcggccgg caccctggaa atctccgacg
    12421 tggaagacgt caaccgcctg ctgaccaccg acgagtcgat cacgcgcggc ctcggcaacc
    12481 cgtgagctga tcgcgtcaac ccgaaccggc cgcgcccagc gccggttcga tcagggtgac
    12541 ggtacgcgca agcgcaccgc gcccgtcttc cagcaatcgc gatgcgttgg ccgccagccg
    12601 caaccgtgcc ggctcgtccg cgaacagtgc ctgcaacgcg tgggccagtg cgcccgcatc
    12661 atccaccacc cgcatcgcct cggcctcgcg caggcgacgg gcgatgtcgg cgaagttgtg
    12721 caggtgcggc ccgctgagga tcgccgtgcc ggtcgccgcc ggctccagca ggttgtgccc
    12781 gccaaccggt tgcaggctgc cgccgacgaa ggcgacggcg gcgcaggcat agaagcgcac
    12841 cagctcaccc agcgtgtcga ccacgaagac ctcggcgtcc ggaccgggcc atgtgtcggt
    12901 gctgcgcgtg ccgaccccga agcccgcggc ctgcgcacgc tctgcgaccg cgcggaagcg
    12961 ctcgggatgc cgcggcgccc acaacagcaa tgcatccggt tgcgagcggc gcagcaggcg
    13021 atgcgcgtcc agtaccgcag cttcctcatc ctcatgggta ctggccgcga tccataccgg
    13081 gcccgcggtg ccgcgatgcg tggcgaagtc ggcggcgaag gccgcggcat cgaccggggc
    13141 catgtcgaac ttgaggttgc cgctgacctg cacccgttcc ggctgcgcgc caagcatcag
    13201 gaaacggcgg gcatcgtcgc gcgattgcgc cgccaccagg cgcacggtgc gcagcgcacg
    13261 gcggatcaag ggcgcgagca cgcgatagcc acgcagggaa cgcgccgaca gccgggcgtt
    13321 gaggatgtgg gtgggaatgc cgcggtcgcg gcaaccgaac agcaggttcg gccacagctc
    13381 ggtttccacg atcagcgcca gcgctgggcg gaaatggtcg aggaagcggc cgaccgcacc
    13441 cggcaggtcg tacggcagat agacgtgggt cacggcatcg ccccacaacg cgcgcacgcg
    13501 ttccgagccg gtcggcgtga tcgtggtgat caccagccgc aggtcgggcc gcaactggcg
    13561 caagcggtcc accagcggcg ccgcggcatt gacctcgccc accgataccg cgtgcagcca
    13621 caacggtgcg gacggaccgc gctccggata attggcgtag cgctcgctcc agcgatggaa
    13681 gtacgcgcgc tggcggaacc cgcgccagat caggtggtag agggtgatcg ggacgagcac
    13741 gtacagcgcg gccgaataga ggccgcgcaa gacgcgttcg gtgaagtcgg cccgcatcgc
    13801 gacaggatag cgaagccacg agcgccagta gaatcccccg gatgaacgac gccgtccgca
    13861 tgccgccgcc actcggccca cgccactggc cggcgtggct cggcatcggc atcgccgcct
    13921 tgctggcgcg cgtgccgtgg tcgttgcaac gcgtgctggg ccgcggcttc ggccgcctgc
    13981 tgatgcgcgt gttcggcagt cgccggcggg tggccgcgcg caacctcgcg ctgtgtttcc
    14041 cggaactgga tgccgatgcg caggcggcgt tgctgcgccg gagtttcgag gaactcggca
    14101 tcggcctgtt cgagttcgcg cgggcctggt ggggctcggt cgagccgatg cgcggcaagg
    14161 tggccgtcga gggcctggag catctcgaag ccgcgcgcgc cggcggtcgc ggggtgatca
    14221 tcatctccgg ccacttcgtg accctggaac tggcggcgcg cctgatgtgc gaccacgcgc
    14281 cgctggccgg catgtaccgg ccgcacgacg gcgcggcgat ggaatgggcg gtcctgcgcg
    14341 ggcgcctgcg ctacgccacc gcgatgttca cccgcgagga attgcgcccg gcgctgcgcc
    14401 acctcaagca gggcggcctg ttgtggttcg cgcccgacca ggacacccgc cgcggcgaca
    14461 gtgtgttcgt gccgttcttc ggccgtcccg cgtacagcct gacctcgacc caccagcttg
    14521 cgcgcctgtc cggcgcggcc gtgctcggct tcgcgcacgt gcgccacgac gatggcggtt
    14581 acacgctgcg attgacgccc gcgttcgagg atttcccgtc gtccgatgcg accgccgaca
    14641 ccgcgcgggt gatggccgcg atcgagacga tgatccgcgc cgcgccggcg caatacctgt
    14701 ggatccaccg ccgtttcaag cgccagcccg accagcccga caacgcgctc tatcgataga
    14761 ccggcggctc gcccggcggc cgcgtcttga accgccgatg cacccacagg tactgctcgg
    14821 gtgccgcctg caggcgctcg ccgatccacg ccatgtagcg cgccgcggta tcggcgtcgt
    14881 ccaccgcggg ccaaccctcc cacgcgggct ggaggtcgat gtgctggcgg ccgtcggcat
    14941 cgcgccgcgc ccacatcagc agcacgcgcc cgccagcgcg ccgcagcacg ccgccgatcg
    15001 cgcccagcgt ggccgcctgc acgccgagga agggcacgaa cacggtgcgc cgtgccgcgt
    15061 tctggtcggg cacgtagaac acgccctgcc ccgcgcgcac ggcagcggcg aagccggcga
    15121 tgtccttctt gtcgtaggtc gcgccggcat aggaacaacg ggcttccgcg atggcggcct
    15181 cgatgcaggg atcgttgttg cggcggacca ggatcggcat cggctggccg ctcgcatcgg
    15241 cgacatggcg catcgccagt tcgatgccgt cgtagtgcgc gccgaccacc accacgccgc
    15301 gtccctcggc cagcgccgca tgcaggtgtt cgatgccgac gatctgcgcc tgccccgcca
    15361 gttgcgcggg cgtggcgaac cagccacgca gggtttccag cacgccggtg gtattcgaga
    15421 ccagcgtatc cgccagcaat gcctctctcg cggcatcgtc caggtggggg aagcagaggg
    15481 cgatgttggt cgcggcgatg cgccggcgct tcgcattgat ccatggcagt cgcaccagcc
    15541 ggcgcgccaa cgcctgcgcc cgtgcgggcg atcgcccggc gacccagcga cccagcgccg
    15601 cgaatgcgcg gccatgccag cagcgcctca tgcgcgcggg ccagggccgg gactgcgcgt
    15661 cgcttcgagg agcttcgcgt acttgaggaa ggcgtagaac gcctgcgtgc gcgcggcgaa
    15721 atacccggcc cagccattga ggacgtagcg cttgcccagg tacagcttga ggaacaccag
    15781 tggcggatac accagcaggc gcaggccgat gaaacgcacg cgccgttcgc gtttgtgttc
    15841 gaccaggccg ctcgaataac gattgacctt ctccacgcga tcggcgagtc gcggctcacc
    15901 gtagtggcgg aagggtgcgt ccaggtcgat gacgcggccc tccaccaccg gggccgcatg
    15961 cacgggtacc gcgttcatcc gcccgcgtgc cttgcggaac agccgcaact gccagttggg
    16021 tcgcgtcgcc ggatgcggcc atcgccagaa cagccattcg cggcgcggca ggcggtagcc
    16081 gtccgcgcgc ggtgcgtcga gctcgcgcag gagcgtcgcg cggccgtcat cggtcagcga
    16141 ttcgtcggca tccagcagca ggatccactc gccttcggcc aggtcgatcg ccgcctgttt
    16201 ctgcgggcca tagccggcga acggttgcga tgcgatgcgc gcaccatgcc tgcgcgcgat
    16261 gtcgagcgtg gcatcggtcg aacccgaatc cagcaacacg agttcgtcgc agaaacgcag
    16321 gctggccagg cacgcctcca gcgtggctgc attgtccagc gtggtcacca ctgcggacag
    16381 gcgaacgccc atctcaatcc tgctcgcggc ccagcaggct gcctgcgtac agcgcggaca
    16441 gcatcagcag cacgccgccc cagaaggtcg agtacgaggc caggtgcgtg ttgagcggga
    16501 acaccgtgac cgccagcgcc agcatcgccg gacgcgcacg gtcgcgggcc acgggatcgg
    16561 cgtagcgcca cgcgcgccac gcgagcgccg cgcccgccag ccacaacaac aggccgatgc
    16621 cgccggtttc gctgaggatt tccaggacca attggtgcgc atgcagggcg gcaccttcgc
    16681 cccatgccgc gatcaccccc ggttgcggat cgcaggccgc gaacgcctgg cggaaaccgc
    16741 gggcgccgac gccgttgacc ggatgctccc gcgccatgca caccgccgcc gaccagatcc
    16801 gcgagcgccc ggacaacgcg gtgtccacgc ccttggaatc ggcggtgagc acatgggtgg
    16861 tgcgctcgat gcgctcgcgc acctgcggca ccaccagcgt ggccgcgacc agcgccagtg
    16921 cgcccagcgc aaagaccatc aacagcttcc tgcggcccag cgtgcgccag ccggtccaca
    16981 gcagcaccag gccataggtc agccacgatg cccgcgagcc cgccagcaat accacgatgc
    17041 cgatgcccac ggctgccacc agccagccga tcctgccgaa gcgccggcgc atcgcgtcca
    17101 gcgcgaacgg cgacaggctg gccaggacga tgccgagctt gagattgcag ggtcccagga
    17161 cgcccgccag gcgatccgcc gccgcaacct gctcggcggt gcacatgccg tgcccgctga
    17221 tcgcgtgctt gaccatgtcg atgccgctga acaacgggct ggcgccagtc gcggcctcga
    17281 tgagcgcatc cagcgtccac gcgccgacga tgatcgccag cccgccgaag gtgcgccggc
    17341 ggccggcatc gtccgccacc gcggcggcca cgatccacag gaacggcagg tagcgcaggt
    17401 cgaccagcgc ctcgcgcaat gcgcgtccgc gatccatcgc gtcgatggcg gagaccgctt
    17461 ccggtaacca ataggcgaag aacagcacgc tggtcagcgc ccaggccggc ccgctgagca
    17521 gcgcattgcc gccacggaat cggctcgcga ccagcctgag caatccggcc aacgcgccca
    17581 gcgcgagcac gccttccgcg tacccgggcg ccggccacaa ggcaacgaag gccagcaccc
    17641 acgccggcgc ccagcgccag ccgtgcaccg cctgggttgc gtcgttcatg cctgcaccag
    17701 tgcgtggtac tgcgccaagg tcgcctcctg catcgcccgc aacgtgtacg gcatcgtagc
    17761 cggtggcacc ggcggattcg cgagcagggc ctgcgcgcac tcccgcaacg ccgtggcatc
    17821 gaacggcagc accgcaccct ccggttgcag ctcgcgcagc agctcgccga cacccccgtg
    17881 atcccagccc agcaccggcc ggccgatgga cagcgcttcg atcacggtgc ggccgaaggc
    17941 ctccggcttg cgcgagagtt gcaggaccag gtcgctcgcc gcgtaggccg tgggcatgtc
    18001 cgctgtcggc tcggtcatcg ccacggcatc ctcgatgctg gctctgcggg tcatttccac
    18061 gagctcgcgg acgtacgcct cccgtcccgc ctgcaccatg ccaggcatcc acagccgcgc
    18121 gtccgtgccc gccccgcgca gcgcagcgag caacgcgatc gcgtctgtat ggcccttcag
    18181 gcgggtgccg cgaccgggca gcaagagcag cggaccctcg ccctcgagtt gcggatgcct
    18241 gcctgcgatg cgcgcgcgcg ccgcgcggtc cggatgcggc atgcggggga aggcagcggg
    18301 gtcgattccg cgcggaatca ccaccaggcg ggacggatcg gtggccggat agtgcgccag
    18361 cagatgatcg cgcaccgtcc gcgagacgca gaccacgcgc tcgccgcggg ccatgatcgc
    18421 gctgtagcgc gagggagaat tcaagccatg cgcggtggtg atgaaacgtg gacgcttgcc
    18481 gtgcgtaccg cgcagcgccc accaacccaa ccacgccggc aaccgcgacc gcgcatgcac
    18541 gatgtcggcg ccggcctcgc gcaagagcgc gcgcaacgcg cgtacgtggc gcagcaccag
    18601 cggcgacttg cggccgatgt cgagggtgag gtgctcggcg ccgatcgcct gcaactgcgc
    18661 cagcaggcgc ccgccggcgg acacgacgat ggcgcgatga ccggcatcga caagcgcttc
    18721 ggcgatctcg agggtggaac gttcgacgcc gccagcgtca agcgccggca gcaattgcac
    18781 cacggtcagg cggcgcatcg cgtcgcggct gcgtcagtcc accagcacgt aggtcgcgcc
    18841 gcagtacggg cactggctgt tgccgccttc ttcctcgatc ggcaggtaca cgcgcggatg
    18901 cgagttccac agcgccatgg acggctgcgg gcagctcagc ggcaagtcgg cgcggcgcac
    18961 ctcgtagcgt tgctgggcat tggcgggcga cgaggcggga gtggtcatgg cgatgtggcg
    19021 cattggcggg aaccgcgcca gtttactgca ggacttcctt tcgccgtcat ccgctgcttg
    19081 ccgtcatccg gcgttcgccg cgatgacgac cctgatcgct caggccggca ggtcgaacag
    19141 caggacgtcg ctggcggctg caatgccgcg aatgaccagc gcatcggctt cctgcacgaa
    19201 gccgagtgcg tcgccagcct tcaacacgcg ctcgccaacc gcgacttcac cggtcgccac
    19261 gtgcagccag tacaagcgcg tgggatcgcg cgatgcagac acctcatcgc ccggctgcag
    19321 gccggcggcg cgcagcgtgg cctgctggcg gatcgcgatc gaaccgtcca cgccgtccgg
    19381 agacgccagc gtcgcccagc gcccgcgacg gtccgcgggc tcgaagcgct gctgtgcgta
    19441 ggcgggttgc gcgttgaggc gatccggctg gatccagatc tgcaggaaat gcaccggcgc
    19501 ggcaccggaa ccggaaccgt tgtattcgct gtgttcgatg ccgtgcccgg cgctcatcca
    19561 ctgcacgtcg ccggcttcga tcatgcccac cgtgccggtg ctgtcgcggt gcgccaacgc
    19621 accctgcagc actacgctga tgatctccat gttggcgtgc cggtgcggcg agaaaccgcc
    19681 acccggggcg accgtgtcgt cgttgatcac gcgcaacgga ccgaagccca tccaggccgg
    19741 gtcgtggtaa tgaccgaagg agaacgtgtg ccggctccgc agccagccca ggtcgacctc
    19801 gccgcgcgcg ctggaggggc gttcgatgat catcgcggca tcacttggcg gcgtcggcct
    19861 tcggcgcagt ggcttcggtg gtgatgcgca gggtcacctc gtcgcccacc atcggcgcga
    19921 acaggcccac gccgaagtcg ctgcgcttga tcgtggcggt ggcgtcgaag ccgatcgccg
    19981 ggaccttctt catcggatgc tcggacgcct tgttgagggt cgcgtcgagg accaccgact
    20041 tggtgatgcc cttgatggtg aggtcgccgg tgatcttcag cttgccgttg ccggcggact
    20101 cgaccttggt gctcttgaag gtcgcggtcg ggaacgtggc gatgtcgaag atgtccgcgc
    20161 tgcgcaggtg cgcgttgaac ttgtcgctga agccgttcag gccggacagc ggcagggtca
    20221 cttgcacgct ggacttgctc acgtcggcgg cgtcgtacac cagggtgcct tcgacgtcgc
    20281 cgaagttcgc gtacgggttg gaatagccga agtggttcca gctggccagc acgttggtat
    20341 gggtgggatc gagcttgtag gtcacgcctg cagcgaaggc attggcgctg gcggcgacga
    20401 gggcagcggt gagcagcagt ttcttgaaca tgggagtctc cggaaaggtt cggtgcgaat
    20461 cagaggatct ggcaggcttc gtcgaagccg aggcgcggcg aacgcgggaa gatgctggcg
    20521 ggatcgccat gacccaggtt gaccaggatg ctggtgcgga tcgacgtacc ggcgaagaat
    20581 gcctcgtcga ccttggcgtt gtcgaagccg gtcatcggcc cgcagtcgag acccagcgcg
    20641 cgcgcggcga ggatcaggta ggcggtctgc aggctgctgt tgcggtttgc gacccacgcc
    20701 aggttggcct cgtcgcgatg ctcgaaccac ggcttcgcat cgacatgcgg gaacagcacc
    20761 ggcagcttgt cgaagaagcg catgtcgtaa ccgacgatgg ccacgcacgg cgccgccatc
    20821 gtcttcttga agttgccctc gctcagcgcc ggacccagct tggccttggc ctcgtccgac
    20881 ttcacgaaca cgatccgcgc cggggtggta ttggcctcgg tcgnnnnnnn nnnnnnnnnn
    20941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnnnnnnnnn nnnnnnnnnn nnngcctcgt ccgacttcac gaacacgatc cgcgccgggg
    21061 tggtattggc ctcggtcggg ccgaacttca gcagcccgta gagctgctgc agggtgtcgt
    21121 cggacacctc gccggtgaac gcgttgtagg tgcgcgcggt gcggaacaac tggtcgagcg
    21181 cggcatcggg caagggctgg gtcatgcgat cctcgtggcg gggtgcgaag gcgcacagtg
    21241 tagagttcca tgggtgaagc gaatgcaccg agatcgcaac ggatcgattc caccagcgca
    21301 acgatcccgg tcgcgcccat gacgtcgacg gtcgcgcttc acgatgaccg cgctggcaac
    21361 acccgccagg ctatggcgct ggcacgcgcg ctggacgccg atgccggcga atgcctcctg
    21421 cagccacgcg caccttggcg ctggctggca ccacgcgtcc tgcctggcgc cacgcatggc
    21481 ttcggcggcg agttcgccgc gctcatcgcg cggccacccg cgatcgccgt tggttgcggc
    21541 cggcaggccg cactggcgac gcggctgttg cgcgcgcgcg gcagccgcgc cgtgcagatc
    21601 ctcgatcccc gcatcgacgc acgacactgg gatctcgtgg tcgcaccgga acacgacggc
    21661 ctgcgcgcgg acaacgtcat ccagatgctg ggcagcctgc acccggtcga cgacttgtgg
    21721 ctggcgcagg cgcgacgtga tgcagcgcac ctcgccgcat tgccctcgcc gcagaccgtg
    21781 ttgctggtcg gtggcccaac ccgccacgcg gcgctggacg atgccggctt cgctgcactg
    21841 cttgcccggc tctcggcgca cgcacaccac gaaagcggca gcttcagcgt cgtggcatca
    21901 cgacgcacac cggatgcctg gcgcgcgagt ttgagcaatg tgtccgctga catacccggc
    21961 atccgctggc gcgacgaacg cgacggcccg aatccctacg ctggcctgct ggcgcatgcg
    22021 aaccgcatcg cgtgcacgcc ggactcggtg aacatgctca gcgaagccgc ggcgacgctg
    22081 gcaccggtgt tcgtgtggtc ggcggacatc gccaccggac gccttcgcgc cttcctcgat
    22141 gcgctccgca gccgtggccg cgtgcgcgat ctcgacgatg cattggcacc gtttgcggtc
    22201 gatccgctgc gcgagacggc gcgcgtggcg agtgaggtgc ggcgacggct ggcgctaccg
    22261 atcgcctgat gcttcgaact gcaatccctg cgcgcgcacc gcatcgcgga tcgcctcgcg
    22321 cgccagtgtg atgcgttcgt cgagggccac tcggcgcgga cgccgatcca gggtgcagcg
    22381 caggccggca tccagcagcg tggcatgcgc gacctgcagg gcggcgcggg tgttggcatc
    22441 gagcaatccg gcatcgtgca gcgcatcgag gatcgccggc gacgcacgtg gcgtgcgcaa
    22501 gcgcgcgtgc gttcggctgt cgcgcagcag caggaactgc agcaggaatt ccaggtcgac
    22561 cagccccccg tcgccctgct tgaggtcgaa cagcgcagcg tcgctgcggt ccagttccgc
    22621 gcgcatgcgc cggcgcatgg cagacacgtc gtcgcgcaac ccatcgaccg ggcgaccgcg
    22681 ggagagcacg tcgccgcgca cgccctcgaa ggcgtcgatc aatgcgacat cgccggcgac
    22741 cgcacgcgcg cgcaccagcg cctggtgttc ccacgtccat gcacgctcgc gctggtagtc
    22801 ggagaagctg gccaggctcg acaccagcag gcctttcgcg ccatcggggc ggaggcgcac
    22861 gtcgatctcg aacaggcgac cggcaccggt gcgggtttcc agcaaggcga cgatcttctg
    22921 gccgagccgg gcgaaccagc gcgcggcatc cagcggtcgc gcgccatccg atgtcgcgcc
    22981 ttcgactgcg tcgtacagga agaccaggtc gagatcggaa ccgaagccga gttcctcgcc
    23041 gcccaggctg ccgtagccga gcaccgcgaa gcgtgcgccg tcgatgccgc cgtgcgctgc
    23101 gtgcatgccg gccagcgcga gcttcaatat gcaatcgacc accgcgtccg ccagccaggc
    23161 gaggcgacgc gcgcactcct gcgcttcgat gcggcgatca agcagcgcca gcgcgatgcg
    23221 gaagctcaag gcctggcgga tctcgttgag tgcgtccagc gctgcttcgg tgtccgcatc
    23281 gccatcgccc agtgcggccg tgcaggcttg gcgcatcgcc tcgcgccccg gcagttcgcc
    23341 gccgatccgc acgtcgagca attcgtcgag cagcaaggga tgcgcggcca gccgttcgcc
    23401 gagcaaggcg ctgcgtgcca gggcgtcgac caaccgatgc agggccaccg gttgttcgtc
    23461 gagcagggcg aggtaactcg cgcggcgcag cagcatgtgc agcaagggca gcaagtggcg
    23521 cagcacggca tcgggatgcg gcgatgcgac cgccgcctgc agcagcgccg gcagcacgcg
    23581 atccaggcgc gcgcgggtgg catcggagag ggtgcgcacc gccggcacgc gggcgaagtc
    23641 ggccaggctg cgatgaaggt cctcgccggc ttcgaagcca gccgcggcca gcaccgcgcc
    23701 atcgcctgca tccggcaggc cgcgccaata cgccgcgagg tcgctgtcgc cgctggccgc
    23761 ccggcgtgcc gccagcaggg cgtcgaattc ggcggagacg cgctcgcgct gcaccgccaa
    23821 cgcctccagc aaggcctgcc agtgcgcata gcccaggccg tcggcgatgc gcgcgcgcaa
    23881 cagcgcaccc gcgggcaagg catgggtctg cgcgtcgccg agcatctgca ggcggttctc
    23941 cagcgtgcgc aggaagcggt aggcctccag caacatggcg ccggtcgcgg catccacgtg
    24001 ccccgcttca accagcgcgc gcatcgccgg cagcaggcgc cgatcgcgca gcgcgggctc
    24061 gcgcccgccg cggatcagct gcaacgcctg caccaggaac tcgatctcgc ggatgccgcc
    24121 cgggccgcgc ttgatgtcgt cggccatgtc cttgcgcgc
//
LOCUS       DAOOCX010000029        23123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1894_length_23123_cov_7.936666, whole genome shotgun sequence.
ACCESSION   DAOOCX010000029 DAOOCX010000000
VERSION     DAOOCX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 23123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23123
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1894_length_23123_cov_7.936666"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..614
                     /locus_tag="PK399_04400"
     CDS             195..614
                     /locus_tag="PK399_04400"
                     /inference="COORDINATES: protein motif:HMM:NF039797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HPW12395.1"
                     /translation="MKTLLVPLLALASLSACATNRMSDTDRLALYQAHAGEPVKQIRN
                     TNAMGWDRIDDQHIVLSMRPTESWLLTVSGPCLDWASGSPVLRLSSTGPYVMAKFDRI
                     VTDGSPASCRIEEIRPIDVKAMRAARDAGSAQASAGT"
     gene            complement(593..865)
                     /locus_tag="PK399_04405"
     CDS             complement(593..865)
                     /locus_tag="PK399_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HPW12396.1"
                     /translation="MARRVFCEVDKIETDGLDFAPWPGEAGKRVFAGIGKPAWQRWLA
                     HQTMLINENRLSPLNPQHRAFLEGEMLKFLFGGGADKPAGYVPADA"
     gene            complement(868..1929)
                     /gene="mutY"
                     /locus_tag="PK399_04410"
     CDS             complement(868..1929)
                     /gene="mutY"
                     /locus_tag="PK399_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPW12397.1"
                     /translation="MAAIETEFAARLLAWFDRHGRHDLPWQHPRTPYRVWLSEIMLQQ
                     TQVSVVIPYFQRFVGALPDLPSLARAPQDDVLALWSGLGYYARARNLHAAAKRCMDLH
                     DGDLPRDLDALIELPGIGRSTAGAILSQAWGDAVPILDGNVKRVLCRLFGIDGWPGLP
                     KVEKQLWTIAGTLLPDARLADYTQAQMDFGATLCTRSDPACAICPLQEACIALREGRA
                     GELPSPKPGKPLPERVAHMLLIEDDARRVLLHRRPPTGVWAALWSLPEHADLAAARAW
                     FDVHAHGDFDAAQAGDPIAHGFSHYRLHIHPHRIAGVRPRHAVGDNHDLRWATRAELA
                     GIGLPAPVRRLLQASNEKE"
     gene            complement(1929..3218)
                     /locus_tag="PK399_04415"
     CDS             complement(1929..3218)
                     /locus_tag="PK399_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12398.1"
                     /translation="MSETAPPRRVGKRLLRLAAILAAVLLVLLLVAGWLLQPQRAGGF
                     LLKQLGNSLGLEISASTIDYRLRGTPQLVLHDVVATRPGDAAALLRAERVFVSLPWRT
                     LRARGQDLTVQRVELDAPALDLPALQRWLATRPPSEETRIPALVDGLRIVRGRIDNDD
                     WRIEDLSIDVPTLHPEQLLRARVRGRYVAPPMAIPADLAIAIAHPRRLLAGAATGVAG
                     IGTLTIANADWQVPAHVFLAGPIRLGKDSALMKPARLGIAARYRSASTDLPFRLGLQG
                     PMAFNSATWRFVPVQLFLDGDGAIPDASARGSVSVGKRLVLHLDGRIAAWPEAWPTLP
                     APLAASTSPMPFALDYKGSIDFADIAALELRRDATRFDARFRLPAVLAWLDDIATGTP
                     LPPLRGSLSTPKLEISGAVLEGVEIELDDPGIPPQAD"
     gene            complement(3215..4378)
                     /gene="ftsY"
                     /locus_tag="PK399_04420"
     CDS             complement(3215..4378)
                     /gene="ftsY"
                     /locus_tag="PK399_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPW12399.1"
                     /translation="MVSWFRRNKPEAETPRRLSTEDLAAAFPDSHQSQPPAAAPPVAP
                     EAAAAPAPAPRPLPVPPPPAVEPAAVVPDVDALPAPAAAGKPGWRQRITSILTRDITE
                     LFGRNPKLDDDLLDEIETALLTADVGVTATTQLVESMRKRMKSREFADAQALLKALRG
                     ELVAMLAPVVRPLRIDASKKPFVILTVGINGAGKTTTIGKLARRYKDEGLGLMLAAGD
                     TFRAAAVAQLQAWGERNGVAVIAQGQNADAASVAFDALRAAKSRGVDVLIADTAGRLH
                     TQGGLMEELAKVRRVLGKLDPDAPHEVLMVIDGTTGQNAINQVREFSKKVQVTGLVVT
                     KLDGTAKGGVVFALAREFGIPIRYAGIGERPEDLRVFDAEAFVDALLPESLTG"
     gene            4473..5096
                     /gene="rsmD"
                     /locus_tag="PK399_04425"
     CDS             4473..5096
                     /gene="rsmD"
                     /locus_tag="PK399_04425"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPW12400.1"
                     /translation="MNGRRNPPRPASTGSVRIIGGRWRGTRLPVADSPGLRPSSDRVR
                     ETLFNWLQPVLPGARVLDLFAGTGVLGLEAVSRGAASATLVEADPRLARALQAEVARL
                     GATSEVAVHPGDALAWLGRQPAQGFDIAFVDPPFAAGLWEPVLAALPAVLAPEAWLYV
                     ESPSDHAPVPSPEWALHRENRTRDVRYALYRRVTLASIDTSSDPQTT"
     gene            5093..>5161
                     /locus_tag="PK399_04430"
     CDS             5093..>5161
                     /locus_tag="PK399_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010372369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HPW12401.1"
                     /translation="MSVATRTAVYPGTFDPITNGHVD"
     assembly_gap    5162..5261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5262..5459
                     /locus_tag="PK399_04435"
     CDS             <5262..5459
                     /locus_tag="PK399_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HPW12402.1"
                     /translation="EFQMASMNRHLIPEVETLFLTPAEQYGFISSSLVREISRLGGDV
                     SGFVPAAVAAALEAQWRKRSG"
     gene            5513..6001
                     /locus_tag="PK399_04440"
     CDS             5513..6001
                     /locus_tag="PK399_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12403.1"
                     /translation="MNTITRLLAIACLALSFAACKKEEAPKAEVAAPLSAPTTDDVTA
                     WRAYVNDVATRNMDGVTNSPFVYFLPGEKSEGFGGLYERLLEKLEQDLGRGILEGNML
                     VFASPAQDKTTEMVETAFKAVPPGSMKGVKVVFVGSPILGERVRTAVEPAGVKYIFVE
                     AK"
     gene            6054..6317
                     /locus_tag="PK399_04445"
     CDS             6054..6317
                     /locus_tag="PK399_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HPW12404.1"
                     /translation="MSLKINELCVNCDVCEPVCPNKAISMGPDIYVIDPALCTECVGH
                     YDEAQCVAVCPVECIDPDPDHPETNAQLLAKLAGLQLHGGKAA"
     gene            6314..8041
                     /gene="ggt"
                     /locus_tag="PK399_04450"
     CDS             6314..8041
                     /gene="ggt"
                     /locus_tag="PK399_04450"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPW12405.1"
                     /translation="MKRRFQILLSSILLLAPLLAFAQPSGHPAKPPGAAIASAHALAT
                     DAGFETLARGGNAFDAAVTVSAVLSVVEPISSGLGGGGFFLLHDAKTGKDVFVDARET
                     APAAATPQAYLDKAGELDRDRAENGPWSAGIPGLPAALVHVAQRHGRLPLAQSLAPAI
                     RIAREGFPVYARLARGYAERREVMERYPGTREVYLRNGQPPKEGDLFRQPELAATLER
                     LAAGGFDGFYRGTTAKRLLAGVKAAGGKWTAEELAGYRVKEREPLRFQYDGWEVVTAP
                     PPSSGGIALAEMLQILGPWDLAKMPPAERVHLVTEAMRRAYRDRTFYLGDPDFVKIPV
                     RTLMDPDYAAGLRATIHPAKATPSDLLSGAPTPLEDEETTHFSIIDADGNRAAVTQTV
                     NLLYGSGLVPAGTGVLLNNEMDDFALKPGTPNAFGVMGFDANAPAPGKRMLSSMTPTF
                     MSNADKVAVIGTPGGSRIITMVLLGILGYADGLDAPQVAALPRYHHQWMPDALGIERN
                     ALPADVIAALRAMGHVVVVPGEDNQGRRSSDAWGNMNTVLWDKRNNTLSGGSDPRSNV
                     GKARVVASP"
     gene            complement(8060..8767)
                     /locus_tag="PK399_04455"
     CDS             complement(8060..8767)
                     /locus_tag="PK399_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12406.1"
                     /translation="MSARILGPLLSCICALLVAQGVAAQARRGHFDDIGRILCAEGAE
                     RYLPGDYYFCAANKALQAGKSGKALALYEESAAWGDKRAMFNLGLLYLRGDDVPRNEA
                     LGLAWLALSAERAGNQLHREVLAGAWTAASPETRSAADALWNRMKPKYADRVALVRAK
                     QRYDHETALLRRELLRDPTIDKWIDGMKPARGGNVLDALDEAAQETILRPIKGLDGTV
                     NVGNPETVRDPSTPPTP"
     gene            8861..9751
                     /locus_tag="PK399_04460"
     CDS             8861..9751
                     /locus_tag="PK399_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW12407.1"
                     /translation="MKLWSILGNSQKLDGGAMFGNAPRALWERWATVDEANRIDLACR
                     ALLASPIEGKTVLFETGIGAFFPPALRERYGVVEPHHVLLDSLDEAGFRHEDIDVVVL
                     SHLHFDHAGGLLAAWREGESPRLLFPNATFVVGQAHWQRALQPHPRDRASFIPELPGL
                     LEASGRLELVDGDWSQALGRSVRFHHSDGHTPGLLLAEIVGPDLVQGEAHGGVVFCAD
                     LIPGRPWVHVPITMGYDRNAELLIDEKRAFLQDKLARDVHLFFTHDPGCALARVVRDE
                     KGRFGTTHEHAELRARAMAA"
     gene            9814..10200
                     /locus_tag="PK399_04465"
     CDS             9814..10200
                     /locus_tag="PK399_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HPW12408.1"
                     /translation="MPKITLLFASLHALLMLGLVLPIARHRHARRIGLGDGGDTLLAR
                     RIRVHGNFIEHVPFALLVLALLELCGLAPTWLWGFGGALLLGRILHALGLSRSGGYSF
                     GRFAGTTLTWLVFLAMALAGLWLAMR"
     gene            complement(10211..10843)
                     /gene="upp"
                     /locus_tag="PK399_04470"
     CDS             complement(10211..10843)
                     /gene="upp"
                     /locus_tag="PK399_04470"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPW12409.1"
                     /translation="MKIIEVRHPLVQHKLGKLRDVGTDPVLFRQLASEIAAMLAYEAT
                     REMPVETRNVPTWAGPLDVAHVRAEKITLVPILRAGLGFLPGVQAMLPAAAVSVIGLR
                     RNEVTHQPETYYERLADHMGERTAILIDPMLATGGSALAAIDMLKAAGCRQVKCLFLV
                     AAPEGLRALEAAHPDIEVYVASVDERLNEDAYILPGLGDAGDRLFGTPVD"
     gene            complement(10918..12411)
                     /locus_tag="PK399_04475"
     CDS             complement(10918..12411)
                     /locus_tag="PK399_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW12410.1"
                     /translation="MTLLDTLAPLRSHGQVRTHGLDDATVLDFAGRDASLAAAINAAA
                     SEYERVRTDFPALLDLDEDAQVREVQSGFVNFYAEDAANPYVALAARGPWIVTLKGSV
                     IYDTGGYGMLGLGHAPAAVIEAMAKPQAMANIMTPHVSQLRFARAMKAEIGQTRGGCP
                     YAAFLCLNSGSESVSLASRIVDANAKTMTDPGARHAGKTIKRLVVKGAFHGRTERPAL
                     YSDSSRKAYVQHLASYRDETSVLTVPAYDMDALRKLFADADANGWFIEAMFLEPVMGE
                     GDPGRSVPVPFYELARELTRAHGAMLLVDSIQAGLRATGYLSIVDYPGFENVAAPDLE
                     TYSKALNAAQYPLSVLAVTAEAAAIYKRGLYGNTMTANPRALDVACAVLAQLTPALRA
                     NIQQRGKQALAMLDALKAELEGRITKVQGTGLLFSCELAPGYKGYGAGSTEEWMREHG
                     IGVIHGGENSLRFTPHFAVTEDELKLVVATVKRALLEGPRQQVDAAA"
     gene            12551..12982
                     /locus_tag="PK399_04480"
     CDS             12551..12982
                     /locus_tag="PK399_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPW12411.1"
                     /translation="MRISKADELLLSLLREDARASTAEIARRLGLSRTTVQNRITRLE
                     QEGVIRGYTVRVEDELERSRIRAHILIMLRPKQMTTVVKALQAMHEVRELHSVSGGHD
                     LVAFAVAANVGEMDVLTDRIGAIDGVERTTTSIILSTKFER"
     gene            13026..14063
                     /gene="queA"
                     /locus_tag="PK399_04485"
     CDS             13026..14063
                     /gene="queA"
                     /locus_tag="PK399_04485"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPW12412.1"
                     /translation="MKKSDFHYELPEALIAQAPLAERSASRLLLVPPGDDALSDLGVR
                     DLPSLLQPGDLLVFNDTRVIPARLFGTKATGGRVEFLIERLLPGNEARAQLGFSKPPK
                     PGSRIALDAGGEVEVLGREDSFWHLRFHVGESLEQWLQHAGRLPLPPYIQREPGADDA
                     ERYQTVFAREIGAVAAPTAGLHFDDALLDALRERGVQFGHVTLHVGAGTFQPMRVEDI
                     REHRMHSEWLNVGAELVQQVRRTRAAGGRVIAVGTTVLRALESALVDGELQPFAGETN
                     IFIFPGYRIRSIDALLTNFHLPESTLLMLVSAFAGKARVFAAYEHAVREQYRFFSYGD
                     AMLLFPEAASQ"
     gene            14302..15426
                     /gene="tgt"
                     /locus_tag="PK399_04490"
     CDS             14302..15426
                     /gene="tgt"
                     /locus_tag="PK399_04490"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPW12413.1"
                     /translation="MSRMDFELLGTDGAARRGRITFPRGTIETPAFMPVGTYGTVKGV
                     LPDQVRDLGAQIILGNTFHLFLRPGLEVIEAHGGLHGFARWDGPILTDSGGFQVFSLA
                     NRRKITEAGVTFAAPTDGSKVFLGPEESMHIQKVLGSDIVMIFDECPPVTVDGAPVDK
                     RVVERSMELSLRWAERSKQAHAGNDAALFGIVQGGVHHDLRSRSAEGLQQIGFDGYAI
                     GGLAVGETEAERNAMLEHTAPQLPTDRPRYLMGVGRPEDLVEAVARGVDMFDCVMPTR
                     NARNGHFFTDAGVVRVRNSQYERDLRPIEEGCDCVACAGGYSRAYLRHLDRCNEMLGP
                     ILGTLHNLRHYQRLMARMRDAIEQGTFGAFRESFYAARRP"
     gene            15507..>15780
                     /gene="yajC"
                     /locus_tag="PK399_04495"
     CDS             15507..>15780
                     /gene="yajC"
                     /locus_tag="PK399_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPW12414.1"
                     /translation="MNPLDFLIPVAHAQAAGASAASPMGGLQTFLFPIILIVIMYFLM
                     IRPQMKRQKEHRAMLDKLAKGDEVITSGGIAGSVAAIGESFVTVEVA"
     assembly_gap    15781..15880
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15881..17584
                     /gene="secD"
                     /locus_tag="PK399_04500"
     CDS             <15881..17584
                     /gene="secD"
                     /locus_tag="PK399_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPW12415.1"
                     /translation="LKKQGVPFASSEIGKDGNLLVRTATDDAQTRAADVVREEVGSGY
                     VVALNQASTVPGWLRAIGARPMFLGLDLRGGVHFLMQVDREAALAKRFEATAEDMRVL
                     LRDNRIRYNSVEPAAGNTVVAVLSPGQDLSKVRPLLARDMPTYQIEATADRISVRIPQ
                     AELDKMLTDAVQQNIGTLSNRINELGVAEPIIQRQGADRIAVQLPGVQDTAQAKRILG
                     ATATLEYRGVVEGNAVEARESGNVPPEARIYNSKTIGPDGKPLPVLLNKRIITSGEQL
                     QNATSFFDSQSGTPAVSVRLNSAGGQRMYDYTKDHVGKSMAVVYIERVPEVKLVDGKE
                     VRTTRVNEQVISVATIQGVLNRDFQTTGLESMKEGADLALLLRAGALAAPMDFVDERT
                     IGPSLGKENIERGLTAVAFSFVFALLFFLVYYRMFGLITCIALLINLLMVFALMSVLG
                     ATMSLPGLAGIALTVGMSVDANVLINERIREELRLGLPPQKAIAEGYERASGTILDAN
                     VTAFLAGLAMFAFGSGPLKGFGMTTMLGIATSAYTAVSVSRGIATLIYGGRKKLKSVA
                     I"
     gene            17597..18559
                     /gene="secF"
                     /locus_tag="PK399_04505"
     CDS             17597..18559
                     /gene="secF"
                     /locus_tag="PK399_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPW12416.1"
                     /translation="MKPFNVFPYDSNIDFMRMRWVSLSVAFLLMVVALGAMGARGFNF
                     ALDFTGGIGVELRYEKKPDVDKVREQLASAGFHGAQVQNFGSGDDLLVRLQADGKQEA
                     GADKASSIGDAVAKAASLAGNPAEKRSSAVISAQVGKDLAIDGIYAVLFVLVGFLGYI
                     SLRFEKKFAVAAIITTLHDVLICVGWFALSGHEFDLNVLAGILSVMGFSINDTIVVFD
                     RVRENFRGMRADPVTILNKSVNQTLSRTVITSFVAFLSMLSLYIYGGDSLRGMAEAMI
                     IGIIIGTVSSIFVACPLLTMGMLKVSKQDLLPKSRDEEALARRP"
     gene            complement(18655..19473)
                     /locus_tag="PK399_04510"
     CDS             complement(18655..19473)
                     /locus_tag="PK399_04510"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPW12417.1"
                     /translation="MQKPVVNVMVKAARAAGGVLLRSMHKLDAINVVEKDRMDYASDV
                     DEQAEKTVIKELRKSYPDAAFLGEEGGAQGKGNAVFVIDPLDGTSNFLHGFPHWCVSI
                     ALVENGEPQHGVIFDPLRNELFTASRGSGAVLNDKRIRVSERKDLPGSLLITGFPPRE
                     RERAPAHLECVRELLRSAEDIRRTGSAALDLAYVACGRSDGYFEAGVKPWDIAAGVLL
                     VREAGGRVCDFRGAATGPMDSRGVHGRHLVAGNQKLSAELQKAIVQSGYAAVFK"
     gene            19581..>20122
                     /locus_tag="PK399_04515"
     CDS             19581..>20122
                     /locus_tag="PK399_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPW12418.1"
                     /translation="MSDPSNSAAGDPPAPAHPAASQLRIVLVGTQHPGNIGSAARAMK
                     TMGLSKLVLVAPEKAPDRDTHAMAAGADDLVEAAPVFATLAEAVADCRWVLGCTARSR
                     RIQLEQLHPRDAAARAVLAAAGGPVALVFGRERTGLDNEELQLCHAAVHIPSDPAFSS
                     LNLAAAVQVLSYELRCALLG"
     assembly_gap    20123..20222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20223..20498
                     /locus_tag="PK399_04520"
     CDS             <20223..20498
                     /locus_tag="PK399_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12419.1"
                     /translation="GEAGGGRTLPPGEDVASHAELEGLFGQLAEALDQIDFHKGRAPA
                     SAMRKLRRLYLRANLDSAEVRLLRGVLADTQRMAWLAGQGSSTGKIE"
     gene            20541..21500
                     /locus_tag="PK399_04525"
     CDS             20541..21500
                     /locus_tag="PK399_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HPW12420.1"
                     /translation="MRRLLPRLVGLLCVLLVLAPLAEAQVPAVAATPTAGGENDASVL
                     VLGRVSDDPKAHYDQLKPLLDYVVPRMASVGIRSGRILMAKDLQQMASYLRRGRVDWV
                     NETAGNAALLERRGVAKSFLVTEREGATRYHSVFFVRRDSPVRSLKDLAGRSVAFQNP
                     YSTSAYYLPAAQLLEQGMTLELLLSPMDKPAPDTVSYLFARTELNITTWVHKRLVDAG
                     VFSNLDWSNPQRMPPSFMQDFRIVGRSDDVPRALMLARHGMDPKVEARLREVLMEAST
                     DPDAGEVLRRFIDTSRFVPINDEDRRALDRLGKGVQRVRSEVE"
     gene            21497..>23123
                     /locus_tag="PK399_04530"
     CDS             21497..>23123
                     /locus_tag="PK399_04530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW12421.1"
                     /translation="MRRPGLSLQSRFYAAILALLAIVVLVMVLLWQRQQSSQHEVSEV
                     TRNAMHGLLSEQVRIDGEAEVRQLADALANPLYYFDLDAVGALARAALREADVDYVLV
                     YDAQGRILHDGSGDIAAYGQPMNDPLAKEVIAATGPHALTRGQLLDVSRPVMIGDERL
                     GGVRIGYSLVGMARDEAEAMDALRGRIDDVGRRSLLWLLLLSAALAVIAIVAAVVVQR
                     LLVQPIQVLGDAARQIESGNFTSPVPVSKRQDELGDLIRTFGRMRESVARHDRDIRRV
                     AYSDALTGLSNRLAFRESLDQRLRALQGAGRQLALVFADLDDFKRVNDTLGHEAGDEV
                     LLQVATRIDRAVQAIRGADAMVARFGGDEFVVLLQAADNEPEGDVRRMVTRLAETLVR
                     ELGEPMLLHGRQVFLGSSVGVALFPDDASSASLLMKNGDIAMYQAKVAGKNCYRFYSR
                     TMDQAVERRVRLEHDLRGAWSRGELSLAYQPIFRTRDRVLVGAEALLRWNHPEDGAIA
                     PSVFIEVAEQSGLIEAIGPEVLRSACAEAAGWPT"
BASE COUNT         3553 a         7704 c         7956 g         3610 t
ORIGIN      
        1 ccgacacggg gattttcaat cccctgctct accaactgag ctacccggcc agtgcagccg
       61 gtcgaacgag ccgcgcatga tacgtgcagc cgcggttccc ggcaacccga gcacgcgctg
      121 aacgggttcg ggcaggtaaa gcacaggtta tgcgccgcgg ggcgatgatc gcgtcagtcc
      181 accggagcat cgccatgaag acccttctgg ttcccctgct tgcccttgcg tcactgagcg
      241 cctgcgccac caaccgcatg agtgacaccg atcgcctggc gctttaccag gcgcatgccg
      301 gcgagcccgt caagcagatc cgcaacacca acgcgatggg ctgggaccgg atcgacgacc
      361 agcacatcgt actcagcatg cggcctaccg aaagctggct gctgaccgtg tccgggcctt
      421 gcctggattg ggccagcggg tcgccggtgc tgcgcctcag ttccaccggg ccgtacgtga
      481 tggccaagtt cgaccggatc gtcaccgatg gttcgcctgc cagttgccgg atcgaggaga
      541 tccgcccgat cgacgtcaag gcgatgcgtg ccgcgcgtga tgcggggagc gctcaggcgt
      601 ccgcaggcac gtaacccgcg ggcttgtcgg cgccgccgcc gaacaggaac ttcagcatct
      661 cgccttccag gaaggcgcga tgctggggat tcagcggcga cagccggttc tcgttgatca
      721 gcatggtctg gtgggcaagc caacgctgcc aggccggttt gccgatgccg gcgaagaccc
      781 gcttgccggc ttcgccgggc cacggggcga agtcgaggcc atcggtttcg atcttgtcca
      841 cttcgcagaa caccctgcgc gccatcgtca ttccttttcg ttggaagcct gcagcaatcg
      901 ccgcaccggg gcgggcaggc cgatcccggc caattccgcg cgcgttgccc agcgcaggtc
      961 gtgattatcg ccgaccgcgt gacgcggccg cacgcccgcg atgcgatgcg ggtggatgtg
     1021 cagccggtag tgactgaaac cgtgggcgat gggatcgccc gcctgcgcgg cgtcgaaatc
     1081 gccatgcgca tgtacgtcga accaggcgcg cgctgctgca aggtcggcgt gctccggcag
     1141 cgaccagagc gcggcccaca cgccggtcgg agggcggcgg tgcaacagca cgcgtcgtgc
     1201 atcgtcttcg atcaacagca tgtgtgcgac gcgttccggt agcggctttc cgggtttcgg
     1261 cgacggcagt tcgccggcgc gcccctcgcg caatgcgatg caagcctcct gcaggggaca
     1321 gatcgcacag gccggatccg accgcgtgca cagggtggcg ccgaagtcca tctgcgcctg
     1381 ggtgtaatcg gccagccggg catcgggcaa cagcgttccg gcgatggtcc acagctgctt
     1441 ttccaccttt ggcaggccgg gccagccgtc gatgccgaac agtcggcaca gcacgcgctt
     1501 gacgttgccg tccaggatcg gcacggcatc gccccaggcc tgcgacagga tcgcgccggc
     1561 ggtgctgcgg ccgatgccgg gcagctcgat caatgcgtcg aggtcgcgcg gcaggtcgcc
     1621 gtcgtgcaag tccatgcagc gcttcgccgc tgcatgcagg ttgcgtgcgc gggcgtagta
     1681 gccgagtccc gaccacagcg ctagcacatc atcctgcggc gcccgcgcca acgagggcag
     1741 gtcgggcagc gcgccgacga aacgctggaa atacgggatc accaccgaca cctgcgtctg
     1801 ctgcagcatg atttcgctca gccacacccg gtacggcgtg cgcggatgct gccagggcag
     1861 gtcatggcgg ccgtggcggt cgaaccaggc cagcaggcgc gccgcgaatt ccgtttcgat
     1921 ggcagccatc aatcggcctg gggcgggatg ccgggatcgt ccagctcgat ttccacgcct
     1981 tccagcacgg cacctgaaat ttccagtttc ggcgtggaca gcgaacctcg gagcggcggc
     2041 aagggcgtgc cggtggcgat gtcgtccagc cacgccagca ccgcgggcaa gcggaaccgt
     2101 gcgtcgaagc gggtggcgtc gcggcgcagt tcgagcgcgg cgatatcggc gaagtcgatg
     2161 ctgcccttgt agtcgagcgc aaacggcatc ggcgaggtcg aagccgccag cggcgcaggc
     2221 aatgtcggcc atgcctccgg ccacgcggcg atacgcccat ccaggtgcaa caccaatcgc
     2281 ttgccgaccg aaacgctgcc gcgcgcgctg gcgtcgggga ttgcgccatc gccatccagg
     2341 aacagctgca ccggcacgaa gcgccaggtc gcgctgttga acgccatcgg tccctgcagg
     2401 ccgaggcgga acggaaggtc ggtggaggcg gagcgatagc gtgcggcgat gcccagccgt
     2461 gccggcttca tcagcgcgga atccttgccc aaccgtatcg ggccggcgag gaagacatgc
     2521 gcgggcacct gccaatcggc gttcgcgatg gtcagcgtgc cgatgccggc gacgccggtc
     2581 gcagcgccag ccagcaagcg ccgcggatgc gcgatggcga tcgccagatc ggccgggatc
     2641 gccatcggcg gcgcgacgta gcggccgcgc acgcgggcgc gcaacaactg ctcgggatgc
     2701 agcgtaggca cgtcgatcga caagtcttcg atccgccagt cgtcgttgtc gatgcggccg
     2761 cggacgatgc gcaggccgtc gaccagtgcc gggatgcggg tttcctcgct gggtggacgc
     2821 gtcgccagcc agcgctgcag ggcaggcagg tcgagcgccg gcgcatccag ctccacccgt
     2881 tgcacggtca ggtcctggcc gcgcgcgcgc agcgtgcgcc acggcagcga gacgaacacg
     2941 cgctccgcgc gcagcagggc tgcggcatcg cccgggcgcg tcgccactac atcgtgcaac
     3001 accagttgcg gggtgccgcg caggcgatag tcgatggtgc tggcgctgat ctccaggccc
     3061 aggctgttgc cgagttgctt cagcaggaac ccgcccgcgc gctgcggttg cagtagccag
     3121 ccggccacta gcagcaggac cagcaggacc gcagcgagga tcgcggccag ccgcagcagg
     3181 cgcttgccga cgcggcgcgg cggcgcggtt tcgctcaccc ggtgagcgac tcgggcagca
     3241 gggcgtcgac gaaggcctcg gcgtcgaaca cccgcaggtc ctcggggcgc tcgccgatgc
     3301 cggcgtaacg gatcgggatg ccgaattcgc gcgcaagcgc gaacaccacg ccgcccttgg
     3361 cggtgccgtc cagcttggtc accaccagtc ccgtcacctg cactttcttg ctgaactcgc
     3421 ggacctggtt gatcgcgttc tggccggtgg tgccgtcgat caccatcagc acttcatgcg
     3481 gggcatccgg atccagcttg cccagcacgc ggcgcacctt ggccagctct tccatcagcc
     3541 cgccctgcgt gtgcaggcgg ccggcggtgt cggcaatcag cacgtccacg ccgcgcgact
     3601 tcgccgctcg cagcgcatcg aacgcgaccg aggccgcatc ggcattctgg ccctgcgcga
     3661 tgaccgccac gccgttgcgt tcgccccagg cctgcagttg cgccaccgcg gcggcgcgga
     3721 aggtgtcgcc ggcggccagc atcaggccca gcccctcgtc cttgtagcgg cgtgccagct
     3781 tgccgatggt ggtggtcttg cctgcaccgt tgatgccgac ggtgaggatc acgaagggct
     3841 tcttcgatgc gtcgatgcgc agcggccgga ccaccggcgc cagcatcgcc accagctcgc
     3901 cgcgcaaggc cttgagcagc gcctgtgcat cggcgaattc gcgcgacttc atccgcttgc
     3961 gcatcgactc gaccaactgg gtggtggcgg taacgccgac atcggcggtg agcagtgcgg
     4021 tttcgatctc gtcgaggaga tcgtcgtcca gcttcgggtt gcgcccgaac agctcggtga
     4081 tgtcgcgggt gaggatgctg gtgatgcgct ggcgccaacc gggcttgccg gcggcggccg
     4141 gggcgggcag tgcgtcgacg tccgggacga cggccgccgg ttcgacggcg gggggcgggg
     4201 gcaccggcaa gggtcgcggt gccggcgcag gcgccgcagc cgcttcaggc gccaccggcg
     4261 gtgccgcggc gggcggctgc gactggtggc tgtcggggaa ggcggcggcc aggtcctcgg
     4321 tggagaggcg gcgcggcgtt tcggcttcgg gtttgttgcg gcggaaccag ctgaccatgc
     4381 gggggatgcg tgcctgcggg gcgcctggca cggtgccgca ggcgatgagc gagaatggcg
     4441 cgcatgttac cacccgcccc tggcccggcc cgatgaacgg gcgccgcaac ccgccgcgcc
     4501 cggcatcgac cgggagcgtg cgcatcatcg gtggacgctg gcgcggcacc cgcctgccgg
     4561 tggccgacag ccccggcctg cgccccagct ccgatcgcgt gcgcgagacc ctgttcaact
     4621 ggctgcagcc ggtgctgccc ggcgccaggg tgctcgacct gttcgccggt accggggtgc
     4681 tgggcctgga agcggtgtcg cggggggcgg cctcggcgac cctggtcgaa gccgacccgc
     4741 ggcttgcgcg tgctctgcag gccgaggtcg cgcgtctggg tgcgaccagc gaggtcgcgg
     4801 tgcacccggg tgatgcgctg gcctggcttg gcaggcaacc ggcgcagggc ttcgatatcg
     4861 ccttcgtgga tccaccgttc gcagcgggtc tctgggagcc ggtgctcgcg gcattgccgg
     4921 cggtcttggc cccggaagca tggctgtacg tggagtcccc atccgaccat gccccggtgc
     4981 cttcgccgga atgggcactg catcgggaaa accgtacgcg cgacgtgcgc tacgccttgt
     5041 accggcgggt tacactcgcc agcatcgaca ccagcagcga tccccagacg acatgagcgt
     5101 ggccacgcgc accgcggtct atccgggcac cttcgatccg atcaccaacg ggcacgtgga
     5161 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaattccag atggcgagca
     5281 tgaatcgcca cctgatcccg gaggtggaga ccctgttcct cacccccgcg gagcaatacg
     5341 gcttcatttc gtcgtcgctg gtgcgcgaga tctcgcgcct tggcggcgac gtgtccggct
     5401 tcgtgccggc ggcggtcgcc gccgcgctcg aggcgcaatg gcgcaaacgg tcgggctgaa
     5461 cccgcgcgcc gccaccttcg tccaatcaga tcgccgtacc aggggaaaca ccatgaacac
     5521 catcacccgc ctgctcgcca tcgcctgcct cgccctgtcg ttcgccgcgt gcaagaagga
     5581 agaggcaccg aaggccgaag tcgccgcgcc gctgtccgca ccgaccaccg acgacgtgac
     5641 cgcatggcgc gcctacgtca acgatgtcgc cacccgcaac atggacggcg tcaccaacag
     5701 cccgttcgtg tacttcctgc ccggcgagaa gagcgaaggg ttcggcggcc tgtacgaacg
     5761 cctgctcgaa aagcttgagc aggacctggg tcgcgggatc ctggaaggca acatgctggt
     5821 gttcgcatcg ccggcgcagg acaagaccac cgagatggtc gagaccgcgt tcaaggcggt
     5881 gccgccgggc agcatgaagg gcgtgaaggt cgtcttcgtc ggcagcccga tcctgggcga
     5941 gcgcgtcagg acggcggtcg agccggccgg cgtgaagtac atcttcgtcg aagcgaagta
     6001 agcgcgggga ttcctccagc cctcctccgc acgcggggga gggagcggaa agcatgtcgc
     6061 tcaagatcaa cgaactctgc gtcaactgcg acgtctgcga gccggtctgc ccgaacaagg
     6121 cgatcagcat ggggccggac atctacgtga tcgatccggc cctgtgcacg gagtgcgtgg
     6181 gccactacga cgaagcccag tgcgtggcgg tctgcccggt cgagtgcatc gaccccgatc
     6241 ccgaccatcc cgagaccaac gcgcagttgc tggccaagct ggccgggctg caattgcatg
     6301 gaggcaaggc ggcatgaagc gtcggttcca gatcctgttg tcctcgatcc tgttgctggc
     6361 gccgttgctg gcgttcgccc agccatccgg ccatccggcg aagccgccgg gcgcggccat
     6421 cgcctccgcg cacgcgctgg ccaccgatgc cgggttcgag accctggcgc gcggcggcaa
     6481 tgccttcgac gccgcggtga ccgtgtccgc ggtgctctcg gtagtggagc cgatcagctc
     6541 cgggctgggt ggtggcggct tcttcctgct gcacgatgcg aagactggca aggacgtgtt
     6601 cgtggacgcg cgcgagaccg cgccggccgc ggccactccg caggcctacc tcgacaaggc
     6661 cggtgaactg gaccgtgacc gcgccgagaa cgggccgtgg tcggccggca tccccggcct
     6721 gccggcggcg ttggtgcacg tggcgcagcg ccatggtcgc ctgccgctcg cgcagtcgct
     6781 tgcgccggcg atccgcatcg ccagggaggg cttcccggtg tacgcgcgcc tcgcccgcgg
     6841 ctatgccgag cgccgcgagg tgatggaacg ctaccccggt acccgcgagg tctacctgcg
     6901 caacggccag ccgccgaaag agggcgacct gttccgccaa cccgagctgg cggccacgct
     6961 ggaacgcttg gcagccggcg gcttcgacgg gttctatcgc ggcaccaccg caaagcgctt
     7021 gctggccggg gtcaaggcgg ccggcggcaa atggacggcc gaggaactgg cgggttaccg
     7081 ggtgaaggaa cgcgagccgt tgcgtttcca gtacgacggt tgggaggttg tcaccgcgcc
     7141 gccgccgtcg tcgggcggga tcgcgctggc ggaaatgctg cagatcctgg ggccgtggga
     7201 cctggcgaag atgccgccgg cggagcgggt gcacctggtg accgaggcca tgcgccgcgc
     7261 ctaccgcgac cgcaccttct acctgggcga tcccgacttc gtgaagatcc cggtcaggac
     7321 gttgatggat ccggattacg ccgcgggcct gcgcgcgacc atccatcctg ccaaggccac
     7381 gcccagcgac ctgttgtcgg gtgcgccgac gccgctggag gatgaggaaa ccacccattt
     7441 ctcgatcatc gacgccgatg gcaaccgcgc cgcggtcacc cagacggtga acctgctgta
     7501 cggctctggg ctggtcccgg ccggcaccgg cgtgctgctc aacaacgaga tggacgactt
     7561 cgcgctgaag ccgggcacgc cgaatgcatt cggcgtgatg ggcttcgatg ccaatgcgcc
     7621 ggcaccgggc aagcgcatgc tgagctcgat gacgcccacg ttcatgtcga atgccgacaa
     7681 ggtcgcggtg atcggcacgc cgggcggcag ccggatcatc accatggtgc tgctgggcat
     7741 cctcggctat gccgatggcc tggacgcgcc gcaggtcgcg gccctgccgc gctaccacca
     7801 ccagtggatg ccggatgcgc tgggcatcga acgcaatgca ttgccggccg atgtgatcgc
     7861 cgcgctaagg gcgatgggcc acgtcgtcgt ggtgcccggc gaggacaacc aggggcgtcg
     7921 ctccagcgat gcctggggca acatgaatac cgtgctgtgg gacaagcgca acaacacgct
     7981 cagcggtggc agcgatcctc gctcgaacgt cggcaaggca cgggtggtgg cgtcgccctg
     8041 atcgcgatcc ggcacgcgct cacggcgtcg gcggtgttga cgggtcgcgg acggtttcgg
     8101 gattgcccac gttcaccgtg ccatccagcc ccttgatcgg gcgcaggatg gtttcctgcg
     8161 cagcttcgtc gagcgcgtcc agcacgttgc caccgcgcgc cggcttcatc ccgtcgatcc
     8221 acttgtcgat cgtcgggtcc cgcagcagtt cgcgccgcag cagcgcggtt tcgtggtcgt
     8281 agcgctgttt ggcgcggacc agggcgaccc ggtccgcgta cttcggtttc atccggttcc
     8341 acagcgcgtc ggcggccgag cgcgtctcag gagaggcggc cgtccatgca ccggcaagga
     8401 cttcgcgatg caactggttg cccgcgcgtt cggcggacaa tgcgagccag gccagcccca
     8461 gggcttcgtt tcgcggcacg tcatcgccgc gcaggtagag cagcccgagg ttgaacatcg
     8521 cgcgcttgtc gccccaggcg gcggactcct cgtacagcgc cagcgccttg ccagacttgc
     8581 ctgcctgcaa tgccttgttc gcggcgcaga aataatagtc gccgggcagg tagcgttccg
     8641 cgccctcggc gcagaggatc cgcccgatgt cgtcgaagtg cccccgtctc gcctgcgccg
     8701 cgacgccctg cgccaccagc aaagcgcaga tgcatgacaa tagagggccg aggatccgtg
     8761 cgctcatcgc tcctccggaa gaaggttgcc cacggcatcc cgtactactc ctgcgacaac
     8821 ggccttgcaa gccgtgccgc gccaaacagg gacgaacgcc atgaagctct ggtcgatcct
     8881 cggcaactcg caaaaactcg atggcggcgc gatgttcggc aacgccccgc gggcgctgtg
     8941 ggaacgctgg gctacggtgg acgaggccaa ccgcatcgac ctggcctgcc gcgcgctgct
     9001 ggccagcccg atcgaaggaa aaacggtgct gttcgagacc ggcatcggcg cgttcttccc
     9061 cccggcgctg cgcgagcgct acggcgtagt cgagccgcat cacgtgctgc tggattcgct
     9121 ggatgaagcc ggcttccgcc acgaggacat cgacgtggtg gtgctgtcgc acctgcattt
     9181 cgaccatgcc ggcggcctgc tggcggcgtg gcgcgaaggc gagtcgccgc gcctgctgtt
     9241 ccccaacgcg accttcgtgg tcggccaggc gcactggcag cgggcgctgc aaccgcatcc
     9301 gcgcgaccgc gccagcttca tcccggaatt gcccggcttg ctggaagcca gcggtcggct
     9361 ggaactggtc gatggcgact ggtcgcaagc gctcggcagg agcgtgcgct tccaccacag
     9421 cgacggccat acgcccggct tgctgctggc cgagatcgtc ggcccggacc tggtgcaggg
     9481 cgaggcgcac ggcggcgtgg tgttctgcgc cgacctgatc ccggggcgcc cgtgggtgca
     9541 tgtgccgatc acgatgggtt acgaccgcaa tgcggaactg ctgatcgacg agaagcgcgc
     9601 gttcctgcag gacaagctgg cccgcgacgt gcacctgttc ttcacccatg atcccggctg
     9661 cgcgctggcg cgggtggtgc gcgacgaaaa gggcaggttc ggcaccaccc atgagcacgc
     9721 ggaactgcgc gcccgcgcga tggcggcctg agcgctggca ttcgaccgca ctcgcgtgca
     9781 tggtgcctgc tcccgccagc ccgaatcgcg acgatgccca agatcaccct gctgttcgcc
     9841 agcctgcatg ccctgctgat gctgggcctg gtgctgccca tcgcgcgcca tcgccacgcg
     9901 cgcaggatcg gcctgggcga tggcggcgac acgctgctgg cgcgcaggat ccgcgtgcac
     9961 ggcaacttca tcgagcacgt gccgttcgcg ctgctggtgc tggccctgct ggagctgtgc
    10021 gggctcgcgc cgacatggct gtggggcttc ggcggcgcct tgctgctcgg ccgcatcctg
    10081 catgcgctcg gcctgtcgcg cagcggcggt tattcgttcg gccgcttcgc cggcaccacg
    10141 ttgacctggc tggtgttcct ggcgatggcg ctggccggcc tgtggctggc catgcgctga
    10201 gggcgcgcga tcaatcgacc ggcgtgccga acagccggtc gccggcatcg cccaggccgg
    10261 gcaggatgta ggcgtcttcg ttgaggcgct cgtcgaccga ggccacgtac acctcgatgt
    10321 cgggatgcgc ggcttccagt gcgcgcaggc cttccggcgc ggcgaccagg aacaggcatt
    10381 tcacctgccg gcagcccgcg gccttgagca tgtcgatcgc cgcaagggcg ctgccgccgg
    10441 tggccagcat cgggtcgatc aggatcgcgg tgcgctcgcc catgtggtcg gccagccgct
    10501 cgtaataggt ttccggctgg tgggtgactt cattgcggcg caggccgatc acgctgaccg
    10561 cggccgccgg cagcatcgcc tgcacgccgg gcaggaagcc caggcccgca cgcaggatcg
    10621 gcaccaacgt gattttttcg gcgcgcacat gcgccacgtc cagggggccg gcccaggtcg
    10681 gcacgttgcg ggtttccacc ggcatctcgc gggtggcctc gtaggccagc attgcggcga
    10741 tctcgctggc caactggcgg aacagcaccg ggtcggtacc gacatcgcgc aacttgccga
    10801 gtttgtgctg gaccagcggg tggcggactt cgatgatctt catgcgcgaa gcctagccca
    10861 caaacagacg ggacggccat ccggccgccc cgtcgcgtcg ccgatcgcgg gtgtgcgtca
    10921 ggccgcggcg tcgacctgct ggcgcgggcc ttccagcagg gcgcgcttga ccgtcgccac
    10981 caccagcttc agctcgtctt cggtcactgc gaaatgcggg gtgaagcgca gcgagttctc
    11041 gccgccgtgg atcacgccga tgccgtgctc gcgcatccat tcctcggtgg agcccgcgcc
    11101 gtagcccttg tagccggggg ccagttcgca ggagaacagc aagccggtgc cctgcacctt
    11161 ggtgatgcgg ccctcgagct cggccttcag tgcgtcgagc atggccaggg cctgcttgcc
    11221 gcgctgctgg atgttcgcgc gcagggcggg ggtgagctgc gcgagcaccg cgcaggccac
    11281 gtccagcgcg cgcgggttgg cggtcatggt gttgccgtac aggccgcgct tgtagatcgc
    11341 cgcggcttcg gcggtgacgg ccagcaccga cagcgggtac tgcgcggcgt tgagtgcctt
    11401 ggaataggtc tccaggtccg gcgcagccac gttttcgaag ccggggtagt cgacgatcga
    11461 gagatagccg gtggcgcgca ggccggcctg gatcgagtcc acgagcagca tcgcgccgtg
    11521 cgcgcgggtg agctcgcgcg ccagttcata gaacggcacg ggcacgctgc ggccgggatc
    11581 gccttcgccc atcaccggtt ccaggaacat cgcttcgatg aaccagccgt tggcgtcggc
    11641 atcggcgaac aacttgcgca gcgcgtccat gtcgtaggcc ggcacggtca gcacggatgt
    11701 ctcgtcgcgg tagctggcca ggtgctgcac gtaggccttg cggctggaat cggaatacaa
    11761 cgccggacgt tcggtgcggc catggaaggc gcccttcacc accagtcgct tgatcgtctt
    11821 gccggcgtgg cgcgcgcccg ggtcggtcat cgtcttggca ttcgcatcga cgatgcgcga
    11881 ggccagcgac accgattccg aaccggagtt caggcacagg aaggcggcgt acgggcaacc
    11941 gccgcgggtc tggccgatct ccgccttcat cgcccgggcg aagcgcagtt gcgaaacatg
    12001 cggggtcatg atgttggcca tcgcctgcgg cttggccatc gcctcgatca ccgcggccgg
    12061 agcgtggccc aggccgagca tgccgtagcc gccggtgtcg tagatcaccg agcccttcag
    12121 ggtcacgatc cacgggccgc gcgcggcaag cgccacgtaa ggattggcgg cgtcttcggc
    12181 gtagaaattg acgaaaccgg attgcacctc gcgcacctgc gcgtcctcgt ccaggtccag
    12241 cagcgccggg aaatcggtgc gcacgcgctc gtattcgctg gcggcggcat tgatcgccgc
    12301 ggccaggctg gcgtcgcggc cggcgaagtc gagcacggtg gcgtcgtcga ggccgtgggt
    12361 acggacctgg ccgtgcgagc gcagcggggc gagggtgtcg agcagggtca tggcgggttc
    12421 tccagcggat gcgcgtccgc ggccggacag cgtgcaaacc gccattatcg cgggatcggc
    12481 gccagtcgaa agggcggatt cgtccagttc gccggcaata tccggcggat cgacggtatg
    12541 atccggcgat atgcgcattt ccaaggccga cgaactcctg ctttccctgt tgcgcgagga
    12601 cgcccgcgcc tccaccgcgg agatcgcgcg gcggctgggc ctgtcgcgga ccacggtgca
    12661 gaaccggatc acccggctgg aacaggaggg cgtgatccgc ggctacacgg tgcgggtgga
    12721 ggacgaactg gagcgcagcc ggatccgcgc gcacatcctg atcatgctgc ggcccaagca
    12781 gatgaccacg gtggtgaagg cgctgcaggc gatgcacgaa gtgcgcgaac tgcattcggt
    12841 cagcggcggc catgacctgg tggcgttcgc agtggccgcc aatgtaggcg agatggacgt
    12901 gctgaccgac cgcatcggcg ccatcgatgg ggtcgagcgc accaccacct cgatcatcct
    12961 gtcgaccaag ttcgagcgct gagccggccc gctacaatgc gcgccccgcc ttgcccccgc
    13021 ttgccttgaa gaaatcggat ttccattacg agttgcccga ggcgctgatc gcgcaggcac
    13081 ctctggccga gcgttccgcc agccgcctgc tgctggtgcc gccgggcgat gacgcgctgt
    13141 ccgacctggg cgtgcgcgac ctgccatcgc tgctgcagcc cggcgacctg ctggtgttca
    13201 acgacacccg cgtcatcccg gcgcgattgt tcggcaccaa ggcgaccggc ggccgggtcg
    13261 agttcctcat cgagcgcctg ctgcccggca acgaggcgcg tgcgcagctg ggtttcagca
    13321 agccgccgaa gcccggcagc cggatcgccc tggacgcagg cggcgaggtc gaagtgctcg
    13381 ggcgcgagga cagcttctgg cacctgcgct tccatgtcgg cgagtcgctg gaacaatggt
    13441 tgcagcacgc cggtcgactg ccgttgccgc cctacatcca gcgcgagccg ggcgcggacg
    13501 atgccgagcg ctaccagacc gtgttcgccc gcgagatcgg cgcggtcgcc gcgcccaccg
    13561 ccggcctgca tttcgacgat gcattgctgg acgccctgcg cgaacgcggc gtgcagttcg
    13621 gccacgtcac cttgcatgtc ggtgccggca cgttccagcc gatgcgggtc gaagacatcc
    13681 gcgagcaccg catgcacagc gaatggttga acgtgggcgc ggagctggtg cagcaggtgc
    13741 ggcgtacgcg cgctgccggc gggcgcgtca tcgccgtagg cactacggtg ctgcgcgcgc
    13801 tcgagagtgc gctggtcgat ggcgaactgc agccgttcgc cggcgagacc aacatcttca
    13861 tcttccccgg ctaccgcatc cgcagcatcg atgcgctgct gaccaatttc cacttgccgg
    13921 aaagcacgct gctgatgctg gtgtccgcgt tcgccggcaa ggcgcgcgtg ttcgccgcct
    13981 acgaacatgc agtgcgcgag cagtaccgct tcttcagcta cggggatgcg atgctgttgt
    14041 tcccggaggc ggcatcgcaa tgagtaaggg cagattgccg aagcacgcgc ggcaggcggc
    14101 gggggcttct acccggctta tgcgaacgtc cgtgttcgat gcgccgccgc gcgcccgcgt
    14161 cgcggccctg cccggcacag ggtgccgggc gttcgcacgc ttgcatcgca tgcgatgcaa
    14221 gccaagcgtg ctcacccatg gcgcgctttc cgaagcccct tccgcccgcc gcccgtgccg
    14281 cgaacatgcg tggtgctgcg catgagccgc atggatttcg agctgctcgg caccgacggt
    14341 gccgcgcgac gcggccgcat cacgttcccg cgcgggacca tcgaaacgcc ggcgttcatg
    14401 ccggtcggca cctacggcac ggtcaagggt gtgctgcccg accaggtccg cgacctcggc
    14461 gcgcagatca tcctcggcaa cactttccac ctgttcctgc ggcccggcct ggaggtcatc
    14521 gaggcgcacg gcggcctgca cggctttgcc cgctgggacg gcccgatcct caccgattcc
    14581 ggcgggttcc aggtgttctc gcttgccaac cgccgcaaga tcaccgaggc cggcgtgacc
    14641 ttcgccgcgc cgaccgacgg tagcaaggtg ttcctcggtc ccgaggaatc catgcacatc
    14701 cagaaagtcc tgggcagcga catcgtgatg atcttcgacg aatgcccgcc agtgaccgtg
    14761 gatggcgcgc cggtcgacaa gcgcgtggtc gaacgctcga tggaactgtc gctgcgctgg
    14821 gcggaacgct cgaagcaggc gcatgcgggc aacgacgcgg cgctgttcgg gatcgtccag
    14881 ggcggtgtgc accacgacct gcgttcgcgc tcggccgaag gcctgcagca gatcggtttc
    14941 gacggttatg cgatcggcgg gctcgcggtg ggcgagaccg aagccgagcg caatgccatg
    15001 ctcgagcaca ccgcgccgca gctgcccacg gatcgccctc gctacctgat gggcgtcggc
    15061 cgccccgagg acctggtgga agcggtggcg cgcggggtgg acatgttcga ctgcgtgatg
    15121 ccgacccgga acgcccgcaa cgggcacttc ttcaccgatg ccggcgtcgt acgcgtgcgc
    15181 aactcgcagt atgagcgcga cctgcgcccg atcgaggagg gctgcgactg cgtcgcctgt
    15241 gccggaggct actcgcgtgc ctacctgcgc cacctggatc gctgcaacga gatgctgggc
    15301 ccgatcctcg gcaccctgca caacctacgc cactaccagc ggctcatggc gcggatgcgc
    15361 gacgcgatcg agcagggaac cttcggggcg ttccgggagt ccttctacgc ggcccgccgg
    15421 ccctgaaagg cctcgcggcg gcccacgtgg cataattccc ggctggcctt ggccaaccca
    15481 tccgcttcgc ctacacggat tcccgcatga acccgctcga tttcctgatt cccgttgccc
    15541 atgcccaagc ggccggcgct tccgccgcct cgcccatggg cgggctgcag accttcctgt
    15601 tcccgatcat cctgatcgtg atcatgtatt tcctgatgat ccgcccgcag atgaagcggc
    15661 agaaggagca ccgcgccatg ctggacaagc tggccaaggg cgacgaggtg atcaccagcg
    15721 gcggcatcgc cggcagcgtg gccgcgatcg gcgagagctt cgtgaccgtc gaggtcgcca
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctgaagaagc agggcgtgcc
    15901 gttcgcgtcc agcgagatcg gcaaggacgg caacctgctg gtgcgcaccg cgaccgatga
    15961 cgcgcagacc cgcgctgcgg acgtggtccg cgaagaagtg ggaagcggct acgtggtggc
    16021 gctcaaccag gcgtccacgg tgcctggctg gctgcgcgcg atcggcgccc ggccgatgtt
    16081 cctgggcctg gacctgcgcg gcggcgtgca cttcctgatg caggtcgacc gcgaggcggc
    16141 gctggccaag cgcttcgagg ccaccgccga ggacatgcgc gtcctgctac gcgacaaccg
    16201 catccgttac aactcggtcg agccggccgc aggcaatacc gtggtcgcgg tcctgagccc
    16261 cgggcaagac ctgagcaagg tgcgtccatt gctcgcgcgc gacatgccga cctaccagat
    16321 cgaagccacc gccgatcgca tcagcgtgcg catcccgcag gcagagctgg acaagatgct
    16381 gaccgatgcg gtccagcaga acatcggcac gctcagcaac cgcatcaacg agctcggcgt
    16441 ggccgagccg atcatccagc gccagggtgc cgaccgtatc gcagtgcaat tgccgggcgt
    16501 gcaggacacg gcgcaggcga agcggatcct gggcgccacc gcgacactgg aataccgcgg
    16561 cgtggtcgaa ggcaatgccg tcgaagcacg cgagtccggc aacgtccccc cggaagcgcg
    16621 catctacaac agcaagacca tcgggccgga tggcaagccg ctgccggtgc tcctcaacaa
    16681 gcggatcatc acctccggcg agcagctgca gaacgcgacc tcgttcttcg attcgcagag
    16741 cggcaccccg gcggtcagcg tgcgcctgaa cagcgctggt ggccagcgga tgtacgacta
    16801 caccaaggac cacgttggca agtcgatggc ggtggtctac atcgagcgcg tgccggaagt
    16861 gaagctcgtc gatggcaagg aagtgcgcac cacccgcgtc aacgagcagg tgatctcggt
    16921 cgccaccatc cagggcgtgc tcaatcgcga tttccagacc accggcctgg agagcatgaa
    16981 ggaaggcgcc gacctggccc tgctgctgcg cgccggcgcg ctggcggcgc cgatggactt
    17041 cgtcgacgaa cgcaccatcg gccccagcct gggcaaggag aacatcgagc gcggcctgac
    17101 cgcggtggcg ttctccttcg tcttcgccct gctgttcttc ctggtgtatt accggatgtt
    17161 cggcctgatc acctgcatcg cgctgctgat caacctgctg atggtgttcg cgctgatgtc
    17221 ggtgctgggg gcgaccatga gcctgcccgg actggccggc atcgcgctga ccgtgggcat
    17281 gtcggtggac gccaacgtgc tgatcaatga gcgcatccgc gaggaattgc gcttggggct
    17341 gcctccgcaa aaggcgatcg cggaaggcta cgagcgtgcg tccggcacca tcctggacgc
    17401 gaacgtcacc gcgttcctgg ccggcctggc gatgttcgcc ttcggctccg gtccgctgaa
    17461 gggcttcggc atgaccacca tgctcggcat cgccacctcc gcctacaccg cggtgtcggt
    17521 ctcgcgcggc atcgccacgc tgatctacgg cggccgcaag aagctcaagt ccgtggccat
    17581 ctgaggggcc cgaccaatga aaccattcaa cgtattcccc tacgacagca acatcgactt
    17641 catgcgcatg cgctgggtgt cgctcagcgt tgccttcctg ctgatggtgg tggcgttggg
    17701 cgcgatgggt gcccgcggct tcaacttcgc cctggacttc accggcggca tcggcgtgga
    17761 actgcgctat gaaaagaagc cggacgtgga caaggtgcgc gagcagcttg ccagcgccgg
    17821 cttccacggc gcgcaggtgc agaacttcgg ctccggcgac gacctgctgg tgcgcctgca
    17881 ggccgacggc aagcaggaag ccggcgccga caaggccagc agcatcggcg atgcggtggc
    17941 caaggcggcg tccctggccg gcaacccggc ggaaaagcgc agcagcgcgg tgatcagcgc
    18001 gcaagtcggc aaggacctgg cgatcgacgg catctacgcc gtgttgttcg tgctcgtcgg
    18061 gttcctgggg tacatctcgt tgcgcttcga gaagaagttc gcggtggcgg cgatcatcac
    18121 cacgctgcat gacgtcctga tctgcgtggg ctggttcgcc ctgagcgggc acgagttcga
    18181 cctcaacgtg ctggccggca tcctgtcggt gatgggcttc tcgatcaacg acaccatcgt
    18241 ggtgttcgac cgcgtccgcg agaacttccg cggcatgcgc gcggatccgg tgacgatcct
    18301 caacaagtcg gtcaaccaga ccctgtcgcg cacggtgatc acgtcgttcg tggcgttcct
    18361 gagcatgctc tcgctgtaca tctacggcgg cgactcgctg cgcgggatgg ccgaggcgat
    18421 gatcatcggc atcatcatcg gcacggtgtc gtcgatcttc gtggcatgcc cgctgctgac
    18481 catgggcatg ctgaaggtca gcaagcagga cctgttgccg aagtcgcgcg acgaagaagc
    18541 cctggcacgc cgcccgtaac cgccccggag gggcaggccc cgtgccggcc cgatgaggat
    18601 ccaaacgaaa aggccgcgca ttgcgcggcc ttttcgctgg cgcgaaccgg cggcttactt
    18661 gaacacggcc gcgtaaccgg actggacgat ggccttctgc aactccgcgc tgagcttctg
    18721 gttgccggcg accaggtggc ggccatgcac gccgcggctg tccatcggcc cggtggcggc
    18781 gccgcggaaa tcgcagacgc ggccgccggc ctcgcgcacc aggagcacgc cggcggcgat
    18841 gtcccagggc ttcaccccgg cttcgaaata gccgtcgctg cggccgcagg ccacgtaggc
    18901 caggtcgagc gcggcggagc cggtgcgacg gatgtcctcg gcgctgcgca gcagttcgcg
    18961 cacgcattcc aggtgggcgg gcgcgcgctc gcgctcgcgc ggggggaagc cggtgatcag
    19021 caacgaaccg ggcaggtcct tgcgctcgct gacgcggatg cgcttgtcgt tgagcaccgc
    19081 gccgctgccg cggctggcgg tgaacagctc gttgcgcaat gggtcgaaga tcaccccgtg
    19141 ctgcggctcg ccgttctcga ccagggcgat cgacacgcac cagtgcggga agccgtgcag
    19201 gaagttgctg gtgccgtcga gcgggtcgat cacgaacacc gcattgccct tgccctgcgc
    19261 gccgccttcc tcgcccagga acgcggcatc cgggtaggac ttgcgcagtt ccttgatgac
    19321 cgttttctcg gcctgctcgt cgacgtcgct ggcgtagtcc atgcggtctt tctcgaccac
    19381 gttgatggcg tcgagcttgt gcatgctgcg caggagcacc ccgccggccg cgcgggccgc
    19441 cttgaccatg acattgacca cgggtttctg catggtgaag cgcctcgaag gggaatgagg
    19501 aaggctctcg gggagcgggg caacggcgaa aaagagcgaa cccggcgcgc gggccggggc
    19561 gcgaagttta ccattgccac atgagtgatc catccaattc cgccgcgggc gacccgcccg
    19621 cgcccgcgca ccccgctgca tcgcagctgc gcatcgtcct ggtcggcacc cagcacccgg
    19681 gcaacatcgg ctcggcggca cgggcgatga agaccatggg cctgtcgaag ctggtgctgg
    19741 tggcgccgga gaaagccccg gaccgggaca cccacgcgat ggccgccggt gccgacgacc
    19801 tggtcgaggc cgcgccggtc ttcgccaccc tggccgaggc ggtcgccgat tgccgctggg
    19861 tgctgggttg caccgcccgc agccggcgca tccagctgga acaactgcac ccgcgcgatg
    19921 ccgcggcacg cgcggtgctg gcggccgcgg gcgggccggt ggcgctggtg ttcggccgtg
    19981 agcgcaccgg cttggacaac gaggaactgc agctctgcca cgccgcggtg cacatcccgt
    20041 cggatcctgc cttcagctcg ctcaacctgg ccgccgcggt gcaggtgctg agttatgaac
    20101 tgcgctgcgc gctgctgggc gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20221 nnggcgaagc cggcggcggg cgtaccctgc cgccgggcga ggacgtggcc agccatgccg
    20281 aactggaagg cctgttcggc cagctcgcgg aggcgctgga ccagatcgat ttccacaagg
    20341 gtcgcgcgcc ggcgtcggcc atgcgcaagc tgcgccggct gtacctgcgc gcgaacctgg
    20401 acagcgccga ggtgcgcctg ttgcgcggcg tgctggcgga tacccaacgc atggcatggc
    20461 tggccgggca gggttccagc acgggaaaaa tcgagtaggc tcggcaaggc aaacagggga
    20521 tccgccacgt gccgccaacg atgcgccgct tgctgccacg cctggtcggc ctgctttgcg
    20581 tgttgctcgt ccttgcgccg cttgccgaag cccaggtgcc cgccgtcgcg gccacgccga
    20641 ccgcgggcgg cgagaacgat gccagcgtgc tggtgctggg ccgggtcagc gacgacccca
    20701 aggcgcatta cgaccaactc aagccgctgc tcgactacgt ggtgccgcgg atggcgtcgg
    20761 tcggcatccg cagcggccgc atcctgatgg ccaaggacct gcagcagatg gccagctacc
    20821 tgcgccgtgg ccgggtcgac tgggtcaacg aaaccgccgg caacgccgcc ctgctcgagc
    20881 ggcgcggggt ggcgaaatcc ttcctggtca ccgagcgcga aggcgcgacc cggtaccaca
    20941 gcgtgttctt cgtccgccgc gacagcccgg tgcgctcgct gaaggacctg gccgggcgca
    21001 gcgtggcctt ccagaacccg tattcgacca gtgcgtatta cctcccggcc gcgcaattgc
    21061 tcgagcaggg gatgaccctg gaattgctgt tgtcgccgat ggacaaaccg gccccggata
    21121 cggtcagcta cctgttcgcg cggactgaac tcaacatcac gacatgggtg cacaagaggc
    21181 tggtcgatgc cggcgtcttc agcaacctgg actggtcgaa cccgcagcgg atgccgccat
    21241 cgttcatgca ggatttccgc atcgtcggcc gcagcgacga tgtcccgcgc gccctgatgc
    21301 tggcccggca cgggatggac ccgaaggtcg aggcgcgcct gcgcgaggtc ctgatggaag
    21361 cctcgaccga tccggacgcc ggcgaagtgc tgcgccgctt catcgacacc tcgcgcttcg
    21421 tgccgatcaa cgacgaggac cggcgcgcgc tggatcgtct cggcaagggc gtgcagcgcg
    21481 tgcgctcgga ggtcgagtga gacggccggg gctcagcctg cagtcgcgtt tctacgcggc
    21541 gatcctggcc ctgctggcga tcgttgtcct ggtgatggtg ctgctctggc agcgccagca
    21601 gagcagccag catgaagtca gcgaagtcac ccgcaacgcg atgcacggcc tgctgtccga
    21661 gcaggtgcgc atcgatggcg aagccgaagt ccgccagttg gccgatgcgc tggccaatcc
    21721 gctgtattac ttcgacctcg acgcggtggg cgcgctggcg cgcgcggcct tgcgcgaagc
    21781 cgacgtcgac tacgtgctgg tgtacgacgc gcagggccgg atcctgcacg acggctccgg
    21841 cgacatcgcc gcatacggcc agccgatgaa cgatccgttg gcgaaggagg tgatcgccgc
    21901 caccggtccg catgcgctga cgcgtgggca actgctcgat gtctcgcgcc cggtgatgat
    21961 cggcgacgaa cgcttggggg gcgtgcggat cggctactcg ctggtcggga tggcccgcga
    22021 cgaggcggag gcgatggacg cgctgcgcgg caggatcgac gatgtcggcc ggcgcagcct
    22081 gctctggctg ttgctgttga gcgcagcgct ggcggtgatc gcgatcgtgg cggcggtcgt
    22141 ggtgcagcgc ctgctggtgc agccgatcca ggtgctgggc gatgccgcgc gacagatcga
    22201 gtccggcaac ttcacctcgc cggtgcctgt cagcaagcgc caggacgaac tcggggacct
    22261 gatccgcacc ttcgggcgca tgcgcgagag cgtggcccgc cacgaccgcg acatccgccg
    22321 cgtggcctac agcgatgcgc tcaccggtct gtcgaaccgg ttggcgttcc gcgagtcgct
    22381 ggaccagcgc ctgcgcgcgc tgcagggggc ggggcgccag ctcgcgctgg tgttcgccga
    22441 cctggacgac ttcaagcggg tcaacgatac cctgggccat gaggccggcg acgaggtgct
    22501 gttgcaggtg gcgacccgga tcgaccgcgc ggtgcaggcg atccgcggcg ccgatgcgat
    22561 ggtggcgcgc ttcggtggcg atgaattcgt ggtcctgctg caggccgccg acaacgagcc
    22621 cgaaggcgac gtgcggagga tggtgacgcg gctggcggaa accctggtcc gcgaactcgg
    22681 cgaaccgatg ctgctgcacg ggcgccaagt gttcctgggc tcgtcggtcg gggtggcgtt
    22741 gttcccggac gatgccagca gcgcctcgct gctgatgaag aacggcgaca tcgcgatgta
    22801 ccaggcgaag gtggccggca agaactgcta tcgcttctac agcaggacca tggaccaggc
    22861 ggtggagcgt cgcgtgcggc tggagcacga cctgcgcggc gcctggagcc gtggcgaact
    22921 gagcctggcc taccagccga tcttccgcac ccgcgaccgc gtgctggtcg gtgccgaggc
    22981 cctgctgcgc tggaaccatc cggaggacgg ggcgatcgcg ccttcggtgt tcatcgaggt
    23041 cgccgagcag agcgggctga tcgaagcgat cggccccgag gtgctgcgca gtgcctgcgc
    23101 cgaggccgcc ggctggccca cgc
//
LOCUS       DAOOCX010000030        22636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1957_length_22636_cov_8.378105, whole genome shotgun sequence.
ACCESSION   DAOOCX010000030 DAOOCX010000000
VERSION     DAOOCX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 22636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22636
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_22636_cov_8.378105"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..245
                     /locus_tag="PK399_04535"
     CDS             33..245
                     /locus_tag="PK399_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPW12422.1"
                     /translation="MRVKQSEQGATPAALPQPPAQKGTGVGTGGVPRPRADASGIGPR
                     PVPKVGRNQPCPCGSRKPYKHCHGDG"
     gene            262..516
                     /locus_tag="PK399_04540"
     CDS             262..516
                     /locus_tag="PK399_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12423.1"
                     /translation="MSRAQPLLDDPVFLRSILGCTERMRQELNRCRSEAVAVADLLAI
                     AETSDWLDAHESPVRRRLDRAETRQRLAAHREAQAQAVSP"
     gene            complement(1031..1552)
                     /locus_tag="PK399_04545"
     CDS             complement(1031..1552)
                     /locus_tag="PK399_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HPW12424.1"
                     /translation="MKSGVESRVKADAHWAEARMRSSPTARAAAGAQAAGMSIRKESP
                     MKSLVLLVCLCPGLASAGTLYRCVGAGGQVSYQAKPCAAGQRMDRTIEFAAVPDSQPM
                     ATTSRGKRSSATGARSSRGSRRAGTSARPKPDPCLQAKAKREQRLAQLGLKRTLADLS
                     RLDEPVRAACRGF"
     gene            1586..1999
                     /locus_tag="PK399_04550"
     CDS             1586..1999
                     /locus_tag="PK399_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HPW12425.1"
                     /translation="MQIGQLATQAGVAIDTVRYYERQGLLPPPERRASGYRQYGQPDI
                     ARLRFIRRAKELGFSLQDIHDLLRLSSQADADRSEVRALAQQRLTDIERKLRELEAMR
                     ATLAHLVGQCSGQGSLEGCPIIDTLVAFDAAATNA"
     gene            1996..3750
                     /locus_tag="PK399_04555"
     CDS             1996..3750
                     /locus_tag="PK399_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HPW12426.1"
                     /translation="MNRMHDIDGTSSPDATRRRLLRGMAIGGAAAGLGLWGGPVWALA
                     TPNQAGVLAGTDFDLRIGATPVDITGRTARAITVNGSLPGPILRWREGDTVTLRVRNT
                     LAETSAIHWHGLLLPANMDGVPGLSFHGIEPGGDYLYRFKVRQHGTYWYHSHSSLQEQ
                     AGVYGAIIIDPIERAPFAYDRDYVVLLSDWTDMDPHRLFARLKKRSDYDNLYKRTAGD
                     FLRDVREQGLSNTLADRKAWGAMRMSPTDLADVNGNTYTYLANGRRADDDWTGLFAPG
                     ERVRLRFVNGAAMTFFDVRIPGLKLTVVAADGQDIRPVDVDEFRIAPAETFDVIVQPE
                     RDEAYVVFAQAMDRSGHARITLAPRMGMRAPSPALDQRALLTMDDMGHGSHAMAPGEM
                     ACGANMDHSTMSMPMPAPMQAHPASETGNPFVDMQTMSPAPRLDDPGIGLRDNGRRVL
                     RYADLRSLHAPEDDREPSREIELHLTGHMERFAWGFDGIKFSDAEPVRLKYGERVRFT
                     LVNDTMMEHPVHLHGMWSDLEDADGKFLVRKHTISIPPGTKRSFRVTADALGRWAFHC
                     HMLMHMEAGMMREVRVEE"
     gene            3750..4646
                     /locus_tag="PK399_04560"
     CDS             3750..4646
                     /locus_tag="PK399_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HPW12427.1"
                     /translation="MRRLSAIALLACVSPAFAQDHSQHASHARHAMPPPPAATTSDDH
                     AQHRDPAPGEDDGGDYRPPALTDADRAAAFPDLQGHDMAAHMDDDPVVWMVMADRLEW
                     QQGDALAWEGSAWLGHDRGRLWLRSEGERERGHATEANVEALWGKPVNAWWDLLAGVR
                     HDIADERRGHDGQDWLALGVQGLAPYKFEVQATAYLGERGQSMLQAEAEYELLLTNRL
                     ILQPRIEAIAHGRTDAMRGIGAGLGEVDAGLRLRYEVRREFAPYIGIDRAYRFGKTAD
                     FARAGGDAAHDTRWVAGVRFWF"
     gene            complement(4653..5348)
                     /locus_tag="PK399_04565"
     CDS             complement(4653..5348)
                     /locus_tag="PK399_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPW12428.1"
                     /translation="MGAVPVARIWLASVDSTAASAIAAGTGWLGEDEAARLRQMTATS
                     RQRSFLAGHWLARQVAADWLQAGTSRMALHRHGDGRPQLRVDGEPSTLSLSLSHSGDW
                     LAIALATVPVGIDVELPRRSRDLRALARFTFSREEVARLDGLEEADYSAAFHRLWTLK
                     EARGKRSGSGLLPGESRRVTSLPSDAGTAEATSWTLGDGALSIAVDPGTQVEIVGGEG
                     LRAPAYWRYRDDR"
     gene            complement(5350..5766)
                     /locus_tag="PK399_04570"
     CDS             complement(5350..5766)
                     /locus_tag="PK399_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12429.1"
                     /translation="MKRVLAVLFATVLALSMAQALARSTPLQAPQTVTLARADGAALD
                     GDATRAAILAGAASAEWTVQPDPRGRLLLRKGVRGKHVMVVEAVYAAGQVRFDYVDSI
                     NLNYRMRASGTWSIHPNYMVWRAQLEDAVRAAAART"
     gene            complement(5805..6245)
                     /locus_tag="PK399_04575"
     CDS             complement(5805..6245)
                     /locus_tag="PK399_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ATPase subunit"
                     /protein_id="HPW12430.1"
                     /translation="MRRLTLALATIATLAIAPTAAARDTRQQFDIATAIAAGKADGTL
                     DGSVQFHFKGARAPAVTDRLGAATTNRKTNAFGKSDARACQWVFLGALKALQEAAKER
                     GANAVVDIESNYKNQPFSSATQYECGAGGLMAGVALKGVYAKVK"
     gene            complement(6266..7522)
                     /locus_tag="PK399_04580"
     CDS             complement(6266..7522)
                     /locus_tag="PK399_04580"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HPW12431.1"
                     /translation="MNAASGQRRVVVTGRGVLGPLGHDWPSVQAWLKTGRNAVRHFPE
                     WGEYKGLNSRLGVPAQPFDLPDHYNRKATRSMGRVALLATRASELALVDAGLLGDPLL
                     KSGKVGISYGSSAGTPSSMSDFGRMLAEFNTQYINATTYIRMMAHTAPVNMGVFFGIT
                     GRIITTSSACTSGSQGIGYAYEAIKAGKQVAMLAGGSEELDVTATTVFDTLFATSSDN
                     DAPETTPRPFDASRNGLVIGEGGCTLVLEDLDHALARGATIHAEIVGYGTNSDGQHVT
                     QPNAATMAQAMRLALEDAGLPPDAIAYVNAHGTATEHGDIAETTATHAVFGERMPISS
                     LKSYTGHTLGACGALEAWVTLEMLREGWFHPTANLRNVDPRCGALDYILPGEAGGGRL
                     IDGEYAMSNNFAFGGINTSLVLRRWS"
     gene            complement(7519..8247)
                     /locus_tag="PK399_04585"
     CDS             complement(7519..8247)
                     /locus_tag="PK399_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HPW12432.1"
                     /translation="MTDRCILVTGASRGIGRAIALRLARDGFDIAVHCRSRRDLAEAV
                     VAEIAALGRAARVLEFDVADRAGCASAIEADIAAHGAYHGVICNAGIARDNAFPAMSG
                     EEWDTVIHTNLDGFYNVLQPCVMPMVRRRKPGRIVTLSSVSGIAGNRGQTNYSASKAG
                     IIGATKAMALELAKREITVNCVAPGLIETEMLPPEILDDAMKMIPLRRVGKPEDVAAA
                     VAYLMSDEAGYVTRQVISVNGGML"
     gene            complement(8244..8708)
                     /locus_tag="PK399_04590"
     CDS             complement(8244..8708)
                     /locus_tag="PK399_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HPW12433.1"
                     /translation="MEAHDAAVERLVPHRGGMLWIDRVLHCDADGVLAEATVHAHHLL
                     ADEDDPGLPAWLGIEYMAQGIAAWAGGRALARGDAVKPGFLLGTRRYQCHRPWLAFGL
                     RLRIECRRELMGDNGLGMFACRLLDGDDEIASANVSVFEPPNPDLYLDDPTP"
     gene            complement(8708..9889)
                     /locus_tag="PK399_04595"
     CDS             complement(8708..9889)
                     /locus_tag="PK399_04595"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HPW12434.1"
                     /translation="MTTFFLNELGIACALGRDVDQVATALFADDTPRGVAVTDAVTGA
                     ATSLGLVPGALPSLDDMPVELRGRNNQLLRLALAPLRNAVAEVVARHGADRVAVVLGS
                     STSGVGESEDAHRYFQAHGTWPEAFHYRQQEMGTPAMFVATELGLRGPAHVISTACSS
                     SAKAMASAARLLKAGMADAVITGGADSLCRFTVAGFSALGSVSVARCNPFSANRNGIN
                     IGEGAALFLMTREPGPVHLAGWGETSDAHHMSAPDPDGKGAERAIRDALARAGIAASD
                     IDYVNLHGTATPQNDAMEAGAVQRTLGVDVPCSSTKPLTGHALGAAGAIEAGLCWIAL
                     ARNPDRRLPPHWWDGEADPALAPIRLVRPGERADAPLRHALSQSFAFGGSNAALILGV
                     G"
     gene            complement(9886..10458)
                     /locus_tag="PK399_04600"
     CDS             complement(9886..10458)
                     /locus_tag="PK399_04600"
                     /inference="COORDINATES: protein motif:HMM:NF023089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HPW12435.1"
                     /translation="MQRLIATLLLSLWLAGCATPQASVAARQALAASMPVLRLPPSAL
                     PVPLALQQRLGFRHGDRRQTVDALVESDGANTRLVIHAQGQVALRLDWDGKELKQQRA
                     PFLPAALDGERVLSDLQLVFWPVDAIAPTLPAGWSLRMEGRRRVLRLGDAIIATVDYP
                     QPLQARLEQHALDYSLDITSSDMESAEAGP"
     gene            complement(10446..11684)
                     /locus_tag="PK399_04605"
     CDS             complement(10446..11684)
                     /locus_tag="PK399_04605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW12436.1"
                     /translation="MSPRIETADILIIGAGPAGSVAAALLRQQGREVLVLEKEQFPRF
                     SIGESLLPQSMAYIERAGMLRAVVEAGFQYKNGAAFAHGDKRTQFDFRDKSSPGWGTT
                     YQVQRADFDKVLADEAARMGASVRYRHEVLAVDVDGERPRIDVRAPDGEAYTVEANFL
                     LDASGFARILPRLLKLEKPSGFPVRGAIFTHVRDNIAPGNDAFDRNKILITVHPRHQD
                     VWYWTIPFSNGRCSLGVVAEQAFLAQYAGSESERLRAIVGEDPGLSAVLADADWDTPA
                     RQIVGYSANVESLWGRNYALLGNAGEFLDPVFSSGVTIAFTSANLAANCLARQADGET
                     VDWEAEYGKPLRDGVDAFRRFVDAWYAGGFQKIIFHPNQNPDIRRMICSILAGYAWDR
                     GNPYVAEARRLRVLEELCSG"
     gene            complement(11681..>11962)
                     /locus_tag="PK399_04610"
     CDS             complement(11681..>11962)
                     /locus_tag="PK399_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12437.1"
                     /translation="QLLLLGLGVDYGIFLLEHEGDGASWLAVCLGAASTLLAFGLLSL
                     SATPALHGFGLTLLFGISLVWLLSPCFRPHARPNASAAALAKAPIIETP"
     assembly_gap    11963..12062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12063..13921)
                     /locus_tag="PK399_04615"
     CDS             complement(<12063..13921)
                     /locus_tag="PK399_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPW12438.1"
                     /translation="MAGASGGRAWRWLAWAWLLAVLALGLQQVMFWRAPAIDTDIMAL
                     LPGATEDARLAAANLRLSEAVTGDVVVLLSARDWPTTRDAAHAFARTIGSGKGLRPAG
                     ENNADSMSTAIAAFAPHRQALLTPTQREWLRSATPDAIADMAMARLYSPVSGGIGRWQ
                     DDPLGLWPAWWQARAGQGMQVRDELVSVRGDDGRDWALLRFKSAQPAFRLDGDASLQT
                     VLDAAAGDARKAAGGDLEMLRGGVPLHAEAAASRAAFEVNTIGLGSLLAVLLLVWLAF
                     RSLRPIVLVGVSLLVGLAAAIAVTSWILGQVHLITLVFGATLAGVAEDYGIHYFACRQ
                     GRPEVPPRPMMRSLLPALALALLTSVLAYLALGIAPFPGLRQMAVFATTALTATFITA
                     VLWFPWLDTGTPRHSRFAARIAGSLAHWPRLRPNLGSGVLALAAVLFIGGGLQRLQAS
                     DDLRGLQTSPTALLQDEIAIGKLLGLPSPAQYFLVEGVDAQAVLRNEERLADALRRQV
                     AAGHLQGWRAVSDTLPSLQRQQADRDLVIPAEALARTRVAAMLGEPAPATTTSAHASL
                     QVEDGLRQPLAAPLRPLWLGAVDGRHASLVMLQGLTPAHLTAVATAANGIEGVR"
     gene            complement(13932..14549)
                     /locus_tag="PK399_04620"
     CDS             complement(13932..14549)
                     /locus_tag="PK399_04620"
                     /inference="COORDINATES: protein motif:HMM:NF039988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPW12439.1"
                     /translation="MNRLRTWLVLIVMLLAMAFPAHAADGDGVRSRLAKPAVLRGQFE
                     QQKQLQGFRNPLKSSGDFLLLRDRGIAWNTRKPFASSTRLTRKRLLATMPDGSTQVLI
                     DAGASPGMAAVNALLMALVAGDLDALATRFTLKETLRADGGWSLALQPRDAALKQAFT
                     RIVLDGDRYVRGVEIIEPAGDRTRIVFSDLRETPPATRQEAAQLD"
     gene            complement(14546..14974)
                     /locus_tag="PK399_04625"
     CDS             complement(14546..14974)
                     /locus_tag="PK399_04625"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005420298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPW12440.1"
                     /translation="MSIEPTVPLVAEIELSPAFHDLDPMDVVWHGHTLKYLELARCAL
                     LQGIDYDYPQMRASGYVWPVVDLRCKYVRSARYGQRLRVRAELTEWEMRMRIDYVIRD
                     AETGEVITRAHTLQVAVAIASGEMCYATPEVFRRRLGLAA"
     gene            complement(14964..16520)
                     /locus_tag="PK399_04630"
     CDS             complement(14964..16520)
                     /locus_tag="PK399_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HPW12441.1"
                     /translation="MPAIPAEPASIMVDGRHPLTIEDVVAVARGTAVARLSDAGDFLA
                     RIGRGADFVDRLIAEDGVVYGVSTGYGDSCTVAIPPALVHELPHHLYAYHGVGLGRFL
                     DADETRAVLLARLVSLAQGSSGVSVALLRQLQALLRHDILPRIPAEGSVGASGDLTPL
                     SYVAAVLCGEREVLHDGAVKPAGEALAAHGLQPLRLRPKEGLAIMNGTAVMTALACLA
                     WQRADYLGRLATRITAFNLVASAGNAHHFDEALFAVKPHPGQQRVAGRLRADLATARP
                     SRNEQRLQDRYSLRCAPHVIGVLQDALPWLRTNIENELNSANDNPIIDADAGMVLHGG
                     HFYGGHIAFAMDSLKNLVANLADLLDRQMALLVDARYNHGLPANLSAARGERAALNHG
                     LKALQISASAWTAEALKLTMPASVFSRSTECHNQDKVSMGTIAARDCLRVIELTEQVV
                     AALLVTARQGMALRRAHEGSAKLSDDAEAMFLDLEARIPLVEEDRALDRDLHGLLAAI
                     RDEAWRLYVD"
     gene            complement(16501..17442)
                     /locus_tag="PK399_04635"
     CDS             complement(16501..17442)
                     /locus_tag="PK399_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPW12442.1"
                     /translation="MSRGHWADLGETTFVGGTWFLYAVYRLLGRLPFRICLYPVVLFW
                     WLGSGPARRASLDYLRRLNAAEGVFRREPGMRQSLAHFFLFGETLLDKMLAIGGRYPR
                     ERIRFSGQQAMLDAAQAGQGGVIATAHMGCLELMQMAARWREGLRVTILVHTAHAQQF
                     NRILARLNPQATVRLLQVTDFSPAMAMALADRVAAGEFIAIAADRVPVHGDRTCRAPF
                     LGAEADFPAGPWLIASLLRCPVWGLSCLHDGDGYASRIELLAEQVTLPRADRQAGLND
                     CAARFSGWLAGCLRQSPYDWFNFYDFWMPHANARHSR"
     gene            complement(17439..18197)
                     /locus_tag="PK399_04640"
     CDS             complement(17439..18197)
                     /locus_tag="PK399_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW12443.1"
                     /translation="MTSTSRPWVVVPVYEHEHAIGHTVDQLLPHGVPILLVDDGSGAS
                     CAAVLRELADRHAGRVSLLRLDRNGGKGAAVMAGMRQAATLGASHILQIDADGQHDTH
                     DVPRFLAESAAHPDAVINGRPVYDESVPLGRLVGRYATHVWVWINTLSLDIADSMCGF
                     RVYPLAATLALLDRESVGTRMDFDIEIIVRLHWAGVPIRTVPTRVTYPMDGVSHFRLW
                     RDNARISAMHTRLFFGMLWRLPRLLVRRVRTVVR"
     gene            complement(18194..19852)
                     /locus_tag="PK399_04645"
     CDS             complement(18194..19852)
                     /locus_tag="PK399_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW12444.1"
                     /translation="MRIPLPRLCSVDATASVSPGIDHAQWRLRVGAWREALTAHAGND
                     VALACDDALEFSAALFGAWQAGVTPWLPGDTLPATLDNLRERGLIFAGDLPGGICAIR
                     SHGSIDARLGALDADTCTLVLFTSGSTGEPAAIRKSLRQLDAEVEALESVFGPLIDAD
                     AVIHGTVSHQHIYGLLFRILWPLSSARAFAPARIAYNEQLTTLGPGPVALVASPAHLK
                     RLPDTQDWQALASALRVVFSSGGPLPVDAALTVHRLWGQAAIEVFGSTETGGVAWRRG
                     DGSRAPWQPLPGVQWRVQDGLLDVRSPHLPSNDWWSTSDRAHDAGAGRFELLGRADRI
                     VKLEERRISLDAIQARLQDSDLLDDVRVLALPGHRILLAVAAVASMKGQALLDGEGRP
                     ALVERLRAWLAGHCDPIALPRRWRFLDALPADARGKTRERDLAALFRPLMPTPDWQLR
                     EHAKATATLLAGTELAAFEGHFPHIHVLPGVVLVDWAVRLGRDAFGVAGDMQCMEALK
                     FQQLVRPGTALTAELDWRPGQLGFRFTSARGVHASGRLRFGEAP"
     gene            complement(19857..20390)
                     /locus_tag="PK399_04650"
     CDS             complement(19857..20390)
                     /locus_tag="PK399_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12445.1"
                     /translation="MRVATALAWLATLLYPLAIWLGLARFEPRWLGLFLLLVASLRAL
                     ASREPVWWLAASGALVLVIATFVLNDGLPLKLYPVLVNVAMLLVFAASLWKPPTVIER
                     LARLRHPDLPPEGVRWVANVTRAWCVFFVANGGIALWTALAASDATWALYNGAIAYVL
                     IGAMLAGEWLLRPRHAG"
     gene            complement(20394..20660)
                     /locus_tag="PK399_04655"
     CDS             complement(20394..20660)
                     /locus_tag="PK399_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPW12446.1"
                     /translation="MDKDEIFQRIAGILHDTFGIERASITPQAMLAGDLDIDSIDAVD
                     LIVQLKPMLGGNLRPEAFKAVRSVQDVVDTVHTMLHAGNVATVK"
     gene            complement(20678..20893)
                     /locus_tag="PK399_04660"
     CDS             complement(20678..20893)
                     /locus_tag="PK399_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPW12447.1"
                     /translation="MSLEDVVADDIDTEAPLFNEGLGLDSIDALELGLALQKRYGVTL
                     AADSEETRRHFASVRSLAAFVAASRSQ"
     gene            complement(20934..21719)
                     /locus_tag="PK399_04665"
     CDS             complement(20934..21719)
                     /locus_tag="PK399_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW12448.1"
                     /translation="MADAFDRAWRTFGTALSFIAFGVGGLLLRVLVFPLVGLFSRDRA
                     TLERRARALIRASFAGHIRVMHGLGVMTFEIRGLERLQRRGLLVLANHPTLVDVVLLV
                     SRLPDADCVVKSRLANNPFTRGPVRATGYICNDNGAGLVDDCIASVRSGRNLLIFPEG
                     TRTPAGERLGPLQRGAANIAVRGRLDVTPVVIRCEPRTLGKGEKWYRVPPRRFHLCMD
                     VLPDLPVSPFLEDGVTEPLAARRLTAALGDIFTGELLRAGAGH"
     gene            complement(21712..22446)
                     /locus_tag="PK399_04670"
     CDS             complement(21712..22446)
                     /locus_tag="PK399_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HPW12449.1"
                     /translation="MEHAIDTDATFSIDAWSAFAAGLESHADWHAWARAPWLPTGEAM
                     PALAEMAPMQRRRVERLGRAALQVAWRCQHDDDRDLPLVFASRHGDLDRTHRMLAELA
                     RDEPLSPTQFGLSTHNAIAAQYSIARLLQGNYLAVSAVRATPEAAVTEALGLLADGAP
                     AVLVVVYDDAVPGDYDVFLDEPDALHAWAWRIVAPRDGLPRISLRAGEPAGSGVLPHS
                     LDVLRFFLAGDASLPGVEGGGWSRHG"
     gene            complement(22561..22636)
                     /locus_tag="PK399_04675"
     tRNA            complement(22561..22636)
                     /locus_tag="PK399_04675"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22600..22602),aa:Glu,seq:ttc)
BASE COUNT         3762 a         7753 c         7667 g         3354 t
ORIGIN      
        1 cgctgaccag ccacgttccg cacacccggt cgatgagggt caaacaatcg gagcagggcg
       61 cgacgcctgc ggctctcccg caaccaccag cgcaaaaagg aacgggagtc ggcacagggg
      121 gcgtgccgcg tccgcgagcg gacgcgagcg gcatcggccc gcgtcctgtg ccgaaggtcg
      181 gacgcaatca gccgtgtccg tgcggaagcc gcaagccgta caagcactgc cacggcgacg
      241 gttgaaggcc gggggtgtcg catgagccgc gcgcagccct tgctcgatga tccggtgttt
      301 ctccggtcga ttctcggttg cactgagcgc atgcgacagg agttgaatcg ttgccgttct
      361 gaggcggtcg cggttgcaga cttgctcgcg atcgcggaga cctcggattg gctcgatgcc
      421 catgaaagtc ctgtccgccg ccggcttgat cgcgccgaga ctcgccaacg cctggccgcg
      481 caccgcgagg cgcaagccca ggcggtgtcc ccatgaggcg gaggtgccca ctgtgggttt
      541 cgatcctgat cgcgagttct ggtgctttct cgtcgcggcg gcgtgttgta cggctctggt
      601 cttggaccat cggccatccg ctactgattg ccctccgcac ggcgtggttc tggacgcgca
      661 atccggtctt gttctttcgg gtgcgcctgc tggctaggcg catgcgggcg ttgcatgccg
      721 aagggctcgg attgctttcg gtttcgtccg gaatggtgct gtgagcgcgg tagcgggtgc
      781 agggcggagc cctgcgaaca agcgccatgg atggcgcgct cttgctctag cggttggacc
      841 cgcgttgacg gaacttccaa aggtggccga acccacctct gaaagcccgc ttttgctttg
      901 cccggaaggg caatccttgc cgacggctgg aaccgtcggc gacactcaaa gccgcacagg
      961 aggcggccac gacatgaaaa cacatctgag ccgacttcgc ataatgccta ttatgtaaac
     1021 gtagaagatg tcagaacccg cgacacgccg ccctgaccgg ctcatccagg cgactcaggt
     1081 ccgccaaggt ccgcttcagt cccaactgcg ccaagcgctg ttcacgcttt gccttcgcct
     1141 gcaggcatgg atccggcttg ggacgcgcac tggtgccagc acggcgtgaa ccacgcgaac
     1201 tgcgcgcgcc agttgcactg gagcgttttc cgcggctggt cgtcgccatc ggctgcgaat
     1261 ccgggaccgc cgcgaactcg atggtgcgat ccatgcgctg gccagccgca cagggtttgg
     1321 cctgatagct gacctggccg ccggcaccca cgcagcggta caaggtgccg gcggacgcaa
     1381 gccccggaca caggcacacg agcaatacga gggatttcat cggcgattcc ttgcggatgg
     1441 acatgccggc agcctgcgcg ccggcggccg cgcgggctgt cggggaactc cgcatccggg
     1501 cctcggccca gtgcgcatca gctttgactc tggactcgac tccagacttc atacttcgcg
     1561 tctaccacgc gtcgagcgca tccccatgca gattggacaa ctcgccacgc aggccggcgt
     1621 cgccatcgac accgtgcgct attacgagcg ccagggcctg ctgccgccac cggagcggcg
     1681 tgcgtccggg tatcggcaat acgggcagcc ggacatcgcg cgactgcgct tcatccggcg
     1741 ggcgaaggaa ctgggcttca gcctgcagga catccacgac ctgctgcgct tgagtagtca
     1801 ggccgatgcc gatcgcagcg aggtgcgtgc gctggcgcaa cagcgattga ccgacatcga
     1861 acgcaagctg cgcgaactcg aggcaatgcg cgccacgctg gcgcacctgg tcggccagtg
     1921 ctccgggcaa ggttcgctgg agggctgtcc gatcatcgac accctggtcg cgttcgatgc
     1981 ggccgcgacg aacgcatgaa ccgcatgcac gacatcgacg gcacctcctc gcccgacgcc
     2041 acgcgccgca ggctgttgcg cggcatggcc atcggcggtg cggcggcagg ccttggcctg
     2101 tggggaggcc ccgtctgggc attggccacg ccgaaccagg ccggcgtgct ggcgggcacc
     2161 gacttcgacc tgcgcatcgg ggcgacgccg gtcgacatca ccggtcgcac tgcgcgcgcg
     2221 atcaccgtca acggatccct gcccggcccg atcctgcgct ggcgcgaggg cgacacggtg
     2281 acgctgcgcg tgcgcaacac gctggccgaa accagcgcga tccattggca tggcctgttg
     2341 ctgccggcga acatggatgg cgtgccgggc ctgagtttcc acggcatcga accgggcggc
     2401 gactacctgt accgcttcaa ggtccggcaa cacggcacgt actggtatca cagccattcc
     2461 agcctgcagg aacaggccgg cgtgtacggc gcgatcatca tcgatccgat cgagcgcgca
     2521 ccgttcgcgt acgaccgcga ctacgtggtc ctgctgtccg actggaccga catggatccg
     2581 caccggctgt tcgcgcggct gaagaagcgc agcgactacg acaacctgta caagcggacc
     2641 gccggcgatt tcctgcgcga cgtgcgcgag cagggactgt cgaacactct cgccgaccgc
     2701 aaggcgtggg gcgcgatgcg gatgagcccg accgacctcg ccgacgtcaa cggcaatacc
     2761 tatacctacc tcgccaacgg ccgccgtgcc gacgacgact ggaccggcct gttcgcgccc
     2821 ggcgagcgcg tgcgcctgcg tttcgtcaac ggcgcggcga tgaccttctt cgatgtgcgc
     2881 atccccggcc tgaaactcac cgtcgtggcc gccgacggcc aggacatccg gccggtcgat
     2941 gtcgatgaat tccgcatcgc gccggcggag accttcgacg tgatcgtgca gccggagcgc
     3001 gacgaggcct acgtggtgtt cgcgcaggcg atggatcgca gcggccatgc gcgcatcacc
     3061 ctcgccccgc gcatgggcat gcgcgcgccc tcccccgccc tcgaccagcg cgccctgctg
     3121 accatggacg acatgggcca cggcagccat gcgatggcgc ccggcgaaat ggcctgcggc
     3181 gcgaacatgg accattcgac gatgtccatg ccgatgccgg caccgatgca ggcgcatccg
     3241 gccagcgaga ccggcaatcc cttcgtcgac atgcagacga tgtcgccggc gccgcgcctg
     3301 gacgatcccg gcatcggcct gcgcgacaac ggccgccgcg tcttgcgcta tgcggacctg
     3361 cgcagcctgc atgcgccgga ggacgatcgc gaaccttcgc gcgagatcga gctgcacttg
     3421 accggacaca tggagcgctt cgcctggggc ttcgacggca tcaagttttc cgatgccgag
     3481 ccggtgcgcc tgaagtacgg cgagcgcgtg cgcttcaccc tggtcaacga cacgatgatg
     3541 gaacacccgg tgcacctgca cgggatgtgg agcgacctcg aggatgccga cgggaagttc
     3601 ctggtgcgca agcacacgat cagcatccca ccggggacca aacggagctt ccgcgtcacc
     3661 gcggatgcac tcggacgatg ggcgttccac tgccacatgc tgatgcacat ggaagccggg
     3721 atgatgcgcg aagtgcgggt ggaggaatga tgcgccgcct gtccgccatc gccttgctgg
     3781 cgtgcgtgtc gcctgcattc gcccaggatc attcgcagca tgcatcgcat gcgcgacatg
     3841 cgatgccgcc gccgccagcg gccacgacgt cggatgacca tgcgcagcat cgcgaccccg
     3901 caccgggcga ggacgatggc ggcgactatc gtccccctgc cctcaccgat gccgaccgcg
     3961 ccgccgcatt ccccgacctg caaggccacg acatggccgc gcacatggac gacgatcccg
     4021 tcgtgtggat ggtgatggcg gatcgactgg agtggcaaca gggcgacgcg ctggcatggg
     4081 aaggcagcgc gtggctcggc cacgaccgtg gacgactgtg gctgcgcagc gaaggcgagc
     4141 gcgaacgggg ccacgcaacc gaagccaatg tcgaggccct gtggggcaag ccagtcaatg
     4201 catggtggga tttactcgcc ggcgtgcgac acgacatcgc tgatgaaaga cgcggccacg
     4261 atggccagga ctggctcgcg cttggggtgc agggcctcgc gccttacaag ttcgaagtgc
     4321 aggccaccgc ctatctcggc gaacgcgggc aatcgatgtt gcaggcggaa gccgaatacg
     4381 aactgctgct gaccaaccgg ttgatcctgc agccgcgcat cgaggccatc gcgcatggcc
     4441 gcaccgatgc catgcgcggg atcggtgcgg ggctgggcga agtggatgcc ggcctgcgcc
     4501 tgcgttacga agtgcgacgc gagttcgcgc cctacatcgg catcgatcgc gcgtatcgct
     4561 tcggcaagac cgcggacttc gcgcgggccg gcggtgatgc ggcgcatgac acacgctggg
     4621 tggcaggcgt gcgcttctgg ttttgacgcc gctcagcgat cgtcgcgata tcgccagtat
     4681 gcaggggcgc gcagcccttc cccgccgacg atctcgacct gcgtgcccgg gtcgaccgcg
     4741 atcgacagcg cgccatcacc cagtgtccaa ctggtggctt cggcggtgcc ggcatcgctg
     4801 ggcagcgacg tcacgcgtcg cgactcgcct ggcaacaggc cgctgccgct gcgtttgccg
     4861 cgcgcttcct tcagcgtcca cagccgatgg aaggctgcgc tgtagtcggc ctcttcgagg
     4921 ccatcgaggc gcgcgacttc ttcacgggag aacgtgaatc gcgcaagtgc acgcaggtcg
     4981 cgcgaacgcc gtggcaattc gacatcgatg ccgaccggca ccgtcgccaa cgcgatcgcc
     5041 agccaatccc cgctgtggct caacgacaac gagagtgtcg acggctcgcc atccacccgg
     5101 agttgcgggc gcccgtcccc atggcgatgc agggccatgc gcgacgtgcc ggcctgcagc
     5161 cagtccgctg cgacctggcg cgccagccaa tggcccgcca ggaaactgcg ctgcctcgac
     5221 gtcgccgtca tctgccgcag ccgggcggcc tcgtcctcgc ccagccaacc ggtgcctgcc
     5281 gcgatggccg acgcggcggt actgtccacc gatgccagcc agatccgcgc gaccggcacg
     5341 gcccccatgt caggtccggg ccgccgccgc gcgcaccgcg tcctcgagtt gggcgcgcca
     5401 gaccatgtag ttcggatgga tcgaccacgt gccgctggca cgcatgcggt agttgaggtt
     5461 gatgctgtcg acgtagtcga aacggacctg gccggcggcg tacaccgcct ccaccaccat
     5521 cacgtgcttg ccgcgcacac ccttgcgcag gagcaatcgc ccgcgcggat cgggctgcac
     5581 cgtccactcc gcggacgccg cgccggcgag gatcgcggcg cgggtggcat cgccatccag
     5641 cgccgcgcca tccgcgcgcg ccagcgtcac tgtctgcggc gcctgcaggg gcgtgctgcg
     5701 tgccagcgcc tgcgccatcg agagggcgag gacggtggcg aacaacacgg ccaggacacg
     5761 tttcatggtg gcgccttgcg gttgcaccgc gatcgtgggg atgctcattt gaccttggcg
     5821 tacacgccct tcagggccac gccggccatc agcccgcccg cgccgcactc gtactgggtc
     5881 gcgctgctga agggctggtt cttgtagttg ctctcgatgt cgaccaccgc attggcgccg
     5941 cgttccttcg ccgcttcctg cagcgccttc aatgcgccca ggaacaccca ctggcaggcc
     6001 cgtgcatcgc tcttgccgaa ggcattggtc ttgcggttgg tggtggccgc gcccaggcga
     6061 tcggtcactg ccggcgcgcg tgcgcccttg aagtggaatt gcacggagcc gtccagcgtg
     6121 ccgtcggcct tgccggcggc gatcgcggtg gcgatgtcga actgctggcg ggtgtcgcgc
     6181 gcagcggcgg tcggggcgat ggccagcgtc gcgatggtgg ccagggcaag ggtcagtcgg
     6241 cgcatgcgtg gattcctctt cggggtcagg accagcggcg caacaccagc gaggtgttga
     6301 tgccgccgaa ggcgaaattg ttgctcatgg cgtattcgcc gtcgatcagg cgaccaccgc
     6361 ccgcttcgcc ggggaggatg tagtcgagcg cgccgcagcg cggatcgacg ttgcgcaggt
     6421 tggccgtcgg atggaaccag ccctctcgca gcatctccag cgtcacccag gcctccagcg
     6481 cgccgcaggc gcccagcgtg tgcccggtgt aactcttcag cgaactgatg ggcatgcgct
     6541 cgccgaagac cgcatgggtc gcggtggttt cggcgatgtc gccgtgttcg gtcgcggtgc
     6601 cgtgcgcgtt gacgtacgca atcgcgtccg gcggcaggcc ggcatcctcc agcgccaggc
     6661 gcatcgcctg cgccatcgtc gcggcattcg gctgggtcac gtgctggcca tcgctgttgg
     6721 tgccatagcc gacgatctcc gcatggatcg tcgcgccgcg cgcaagcgcg tggtcgaggt
     6781 cttccagcac cagcgtgcaa ccaccctcgc cgatcaccag gccattgcgc gaggcatcga
     6841 acgggcgcgg cgtggtctcc ggcgcatcgt tgtcgctgct ggtcgcgaac agggtgtcga
     6901 acaccgtggt cgcggtgacg tcgagttctt ccgacccgcc ggcgagcatc gccacctgct
     6961 tgccggcctt gatcgcctcg taggcgtagc cgatgccctg gctgccggag gtacaagcgc
     7021 tcgaggtggt gatgatgcgc ccggtgatgc cgaagaacac gcccatgttc accggcgcgg
     7081 tatgcgccat catccggatg tacgtggtcg cgttgatgta ctgggtgttg aactcggcga
     7141 gcatgcggcc gaagtcgctc atcgacgacg gcgtgcccgc cgacgatccg taggaaatgc
     7201 cgaccttgcc gctcttgagc aacggatcgc cgagcaggcc ggcatcgacc agggcgagct
     7261 cgctggcgcg cgtcgccagc aaggcaaccc ggcccatcga gcgcgtcgcc ttgcggttgt
     7321 agtgatcggg caagtcgaac ggctgcgcgg gcacgccgag ccgcgaattc aggcccttgt
     7381 attcgcccca ttcggggaag tggcgcaccg cattgcgacc ggtcttcagc catgcctgca
     7441 cgctcggcca gtcgtggccc agcggcccca gcacgccacg accggtgacg acgacccggc
     7501 gctgtccgct tgccgcgttc acaacatgcc tccgttgacc gagatgacct ggcgggtgac
     7561 ataacccgcc tcgtcggaca tcaggtacgc caccgcagcg gcgacatcct cgggcttgcc
     7621 gacgcggcgc agcgggatca tcttcatcgc gtcatcgagg atctcgggcg gcagcatctc
     7681 ggtttcgatc aggccggggg cgacgcagtt gacggtgatt tcgcgcttgg ccagttccag
     7741 cgccatcgcc ttggtcgcgc cgatgatgcc ggccttggac gcactgtaat tggtctgccc
     7801 gcgattgccg gcaatgccgg acaccgagga cagggtcacg atgcggccgg gcttgcgccg
     7861 ccgcaccatc ggcatcacgc agggctgcag cacgttgtag aagccgtcca ggttggtgtg
     7921 gatgaccgtg tcccattctt cgccggacat ggccgggaag gcgttgtcgc gggcgatgcc
     7981 ggcattgcag atgacgccgt ggtacgcacc gtgcgcggcg atgtcggcct cgatggcgga
     8041 cgcacaaccg gcgcgatcgg caacatcgaa ttcgagcacg cgggcagcgc ggccgagcgc
     8101 tgcgatctcg gcaacgacgg cctccgccag gtcgcgccgg ctgcggcaat gcacggcgat
     8161 gtcgaagccg tcgcgggcga ggcgcagcgc gatcgcgcgg ccgatgccgc ggctggcgcc
     8221 ggtgacgagg atgcagcggt cggtcatggg gtcgggtcgt cgagatagag gtcgggattg
     8281 ggcggttcga acacggagac attcgcgctg gcgatttcgt cgtcgccatc cagcaggcgg
     8341 caggcgaaca tccccaggcc attgtcgccc atcagttcgc gccggcattc gatgcgcagg
     8401 cgcaggccga acgcgagcca cgggcgatgg cactggtagc ggcgcgtgcc cagcaggaag
     8461 ccgggcttga ccgcgtcgcc gcgggccagc gcacgaccgc cggcccacgc ggcgatgccc
     8521 tgcgccatgt actcgatgcc gagccacgcc ggcaggccgg ggtcgtcttc atcggccagc
     8581 aggtggtgcg cgtgcacggt ggcctcggca aggaccccgt cggcatcgca gtgcaggaca
     8641 cgatcgatcc agagcatgcc gccgcgatgc ggcaccaggc gttcgacggc ggcgtcatgc
     8701 gcttccatca gccgaccccc aggatcaggg cggcgttgct gccgccgaat gcgaaggact
     8761 ggctcagggc atggcgcaat ggcgcgtcgg cacgttcgcc cggacgcacc aggcgaatcg
     8821 gtgcgagtgc gggatcggcc tcgccgtccc accagtgcgg cggcaggcgt cgatccggat
     8881 tgcgtgccag tgcaatccag cacaacccgg cttcgatcgc gcccgccgcg cccagcgcat
     8941 ggccggtcag cggcttggtg gaactgcaag gcacgtcgac gcccagcgtg cgctgcaccg
     9001 cgcccgcttc catggcgtcg ttctgcggcg tggcggtgcc atgcaggttc acgtagtcga
     9061 tgtccgaggc ggcgatgccg gcgcgcgcca gcgcatcgcg gatcgcgcgt tcggcgccct
     9121 tcccgtccgg atccggcgcg gacatgtggt gggcatccga ggtttcgccc cagccggcca
     9181 ggtgcaccgg gccgggctcg cgggtcatca ggaacagcgc ggcgccctcg ccgatgttga
     9241 tcccgttcct gttcgccgag aacggattgc agcgcgccac cgacacggaa cccagtgcgc
     9301 tgaaccccgc caccgtgaac cggcacagcg aatccgcgcc gccggtgatg accgcatcgg
     9361 ccatgccggc cttcagcagc cgtgccgcgg aggccatcgc cttcgcactg gacgagcacg
     9421 cggtggagat cacatgggcg gggccgcgca ggccgagctc ggtggccacg aacatcgcgg
     9481 gcgtgcccat ctcctgctgc cggtaatgga acgcctccgg ccatgtgccg tgcgcctgga
     9541 agtaccggtg cgcgtcttcg ctttcgccga cgccggaggt gctggaaccc aacaccaccg
     9601 cgacgcggtc agcgccatgt cgcgccacga cctcggcaac ggcattgcgc aggggcgcca
     9661 acgcgaggcg caacaactgg ttgttgcggc cgcgtagttc gaccggcatg tcgtcgagcg
     9721 acggcaatgc gccgggcacc aggccgagcg aggtggcggc gccggtgacc gcatcggtca
     9781 ccgccacgcc acgcggtgtg tcatccgcga acagcgcggt cgcgacctgg tcgacatcgc
     9841 ggccgagcgc gcaggcgatg ccgagttcgt tgaggaagaa cgtcgtcacg gtccggcctc
     9901 tgcggactcc atatcactgg aggtgatgtc cagcgaatag tccaatgcgt gttgctccag
     9961 tcgcgcctgc aagggctgcg gataatcgac ggtggcgatg atggcgtcgc ccaggcgcag
    10021 cacgcggcgc cggccttcca tgcgcaagga ccagccggca ggcagcgtcg gcgcgatggc
    10081 gtcgaccggc cagaacacca gttgcaggtc ggacagcacg cgctcgccgt ccagtgccgc
    10141 cggcaggaat ggggcgcgtt gctgcttcaa ttccttgcca tcccaatcca gtcgcaatgc
    10201 gacctggccc tgcgcgtgga tgaccaggcg cgtattcgcg ccatcgctct cgaccaatgc
    10261 atccacggtc tgccggcggt cgccatggcg gaatcccagg cgctgctgca gggccagcgg
    10321 aacgggcaat gcggacggtg gcaaccgcag caccggcatc gacgccgcca gcgcctggcg
    10381 cgcggccacg ctcgcctgcg gcgtggcgca accggccagc cagagcgaca acagcaacgt
    10441 ggcgatcaac cgctgcacag ttcctccagc acgcgcagcc ggcgcgcttc ggcgacatag
    10501 ggattgccgc gatcccacgc ataacccgcc aggatcgagc agatcatccg gcggatgtcg
    10561 ggattctggt tcgggtggaa gatgatcttc tggaagccgc cggcatacca cgcgtccacg
    10621 aagcggcgga acgcatccac gccatcgcgc agcggcttgc catactcggc ttcccaatcg
    10681 accgtctcgc catctgcctg gcgtgccagg cagttggccg ccaggttggc cgaggtgaag
    10741 gcgatggtga cgccggacga gaacaccggg tcgaggaatt cgccggcatt gccgagcagc
    10801 gcgtagttcc ggccccacaa cgactcgaca ttcgcggagt aacccacgat ctgccgtgcc
    10861 ggcgtgtccc agtcggcatc cgcgagcacc gcggacagcc ccgggtcctc gccgacgatg
    10921 gcgcgcaggc gttcgctttc gctgcctgcg tactgcgcca ggaaggcctg ttcggcgacc
    10981 acgcccagcg agcagcgccc gttcgagaac gggatcgtcc agtaccagac atcctggtgc
    11041 cggggatgca cggtgatcag gatcttgttg cggtcgaagg cgtcgttgcc cggcgcgatg
    11101 ttgtcgcgca catgggtgaa gatcgcaccg cggacgggga atcccgatgg cttttcgagc
    11161 ttcagcaggc gcggcaggat ccgggcgaag ccgctggcgt cgagcaggaa gttggcctcg
    11221 acggtgtacg cctcgccatc cggtgcgcgc acgtcgatgc gcggacgctc gccgtcgaca
    11281 tcgaccgcca gcacctcatg ccggtaacgc acgctggcac ccatgcgcgc agcttcgtcg
    11341 gccaatacct tgtcgaaatc ggcgcgctgc acctggtagg tcgtgcccca gcccggcgag
    11401 gacttgtcgc ggaaatcgaa ctgggtgcgt ttgtcgccat gcgcgaacgc ggcgccgttc
    11461 ttgtactgga agccggcctc gacgaccgcg cgcagcatgc ccgccctctc gatgtaggcc
    11521 atgctctgcg gcaacaggct ctcgccgatc gagaagcgcg ggaactgctc cttctccagc
    11581 accagcacct ccctgccctg ctggcgcaac agcgccgcgg cgacgctgcc ggcggggccg
    11641 gcgccgatga tgaggatgtc ggcggtttcg atgcgtggac tcatggcgtc tcgatgatgg
    11701 gtgccttcgc aagcgcggct gcggatgcgt tcgggcgggc gtgcgggcgg aaacaggggg
    11761 acagcagcca gaccaggctg atcccgaaca acagggtcaa tccgaaaccg tgcaatgccg
    11821 gcgtggccga caacgacaac aggccgaacg cgagcagcgt gctggccgcg cccaggcaga
    11881 ccgcgagcca gctggcgcca tcgccttcgt gttcgagcag gaagatgccg tagtccacgc
    11941 ccaggccgag cagcagcaac tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nncgcacgcc ttcgattccg ttcgcagccg tcgccacggc ggtcaagtgc gcgggcgtga
    12121 gtccctgcag catcacgagg ctggcgtggc gaccatcgac ggcgcccagc cacaacggac
    12181 gcagtggcgc ggcgagcggt tggcgcagtc cgtcctcgac ctgcagcgac gcatgggccg
    12241 atgtcgtcgt tgcaggcgca ggctcgccga gcatcgccgc gacgcgggtg cgcgccaacg
    12301 cttcggcggg aatgaccagg tcgcgatctg cctgctgccg ctgcagcgac ggcaacgtat
    12361 ccgagaccgc tcgccacccc tgcaggtggc cggcagcgac ctgccgtcgc agggcatcgg
    12421 cgaggcgctc ctcgttgcgc agcaccgcct gcgcatccac accttcgacc aggaaatact
    12481 gcgccggact gggcaagccc agcaatttgc cgatcgcgat ttcatcctgc aacaacgccg
    12541 tgggcgaggt ctgcaagccg cgcaagtcat cgctggcctg caggcgctgc aggccgccgc
    12601 cgatgaacaa cacggccgcc aacgccagca cgccactgcc caggttcggc cgcaggcgcg
    12661 gccagtgcgc caggctgccg gcgatgcgtg cggcgaagcg gctgtgccgc ggtgtcccgg
    12721 tatccagcca cgggaaccac aacaccgcgg tgatgaaggt cgcagtcaac gccgtcgtcg
    12781 cgaacaccgc catctgtcgc aagccgggga atggcgcaat gccgagggcc aggtaggcca
    12841 gcacgctggt cagcaaggcg agcgccaatg ccggcaacag gctgcgcatc atcggtcgcg
    12901 gcggcacctc gggacggccc tgccggcagg cgaaataatg gatgccgtag tcctcggcca
    12961 ccccggccag ggtggcgccg aacaccaggg tgatcaggtg cacctggcca aggatccagc
    13021 tggtcaccgc gatcgccgcg gccaggccga ccagcagcga gacgcccacc agcacgatcg
    13081 gccgcaggct gcggaacgcc agccagacca gcagcagcac cgccagcagc gacccgaggc
    13141 cgatggtatt gacctcgaat gcggcgcgcg atgctgcggc ttccgcatgc aggggcacgc
    13201 cgccacgcag catctcgagg tcgccgcctg cggccttgcg cgcatcgcca gcggcggcgt
    13261 ccagcacagt ctgcagcgag gcgtcgccat ccagccggaa tgccggttgc gcgctcttga
    13321 agcgcaacaa ggcccagtcg cggccgtcat cgccacgcac ggacaccagc tcatcgcgca
    13381 cctgcatgcc ctgcccggcg cgtgcctgcc accaggccgg ccacagtccg agcggatcgt
    13441 cctgccagcg tccgatgccg ccgctgacgg gtgagtacag acgggccatc gccatgtcgg
    13501 cgatggcgtc gggcgtggcc gagcgcaacc actcgcgctg cgtcggcgtc agcagggcct
    13561 ggcgatgcgg tgcaaacgcc gcgatggccg tggacatcga atcggcattg ttctcgcctg
    13621 ccggtcgcaa ccccttgccg gagccgatcg tccttgcgaa ggcgtgggcg gcatctcgcg
    13681 tggtgggcca atcgcgcgcc gagagcagga ccaccacatc gccggtgacc gcctcggaca
    13741 accgcaggtt ggccgcggcc aaccgcgcat cctcggtggc accgggcaac aaggccatga
    13801 tgtcggtgtc gatcgccggt gcacgccaga acatgacctg ctgcagccca agcgcaagca
    13861 ccgcaagcag ccaggcccag gccagccagc gccaggcgcg gccacccgac gcgcctgcca
    13921 tggcaggccg atcagtcaag ctgcgccgcc tcttgccggg tcgcgggcgg cgtttcgcgc
    13981 aagtcgctga acacgatccg cgtgcggtcg cccgcgggtt cgatgatctc cacgccgcgc
    14041 acgtaacggt cgccgtccag cacgatgcgg gtgaacgcct gcttcaaggc ggcatcgcgc
    14101 ggttgcaggg cgagcgacca gccgccgtcc gcgcgcaggg tttccttgag ggtgaagcgc
    14161 gtggccagcg catccagatc gccggccacc aatgccatca gcaaggcgtt gaccgccgcc
    14221 atgccgggcg atgcaccggc atcgatcaag acctgggtgc tgccgtccgg catggtcgcg
    14281 agcaggcgct tgcgggtcag ccgggtcgag gatgcgaacg gcttgcgcgt gttccaggcg
    14341 atgccgcgat cgcgcagcaa caggaaatcg ccgctcgact tcagcgggtt gcggaagccc
    14401 tgcaactgct tctgctgctc gaactgcccg cgcaacaccg ccggcttggc caggcgcgaa
    14461 cgcacgccat cgccatcggc ggcatgcgcg gggaatgcca tggcgagcag catcacgatc
    14521 aacaccagcc aggtgcgcag cctgttcatg cggcgagccc caggcgacgc cggaacacct
    14581 cgggcgtggc gtagcacatc tcgccgctgg cgatcgccac cgcgacctgc aaggtatgcg
    14641 cgcgggtgat gacctcgccg gtctcggcat cgcggatcac gtagtcgatg cgcatgcgca
    14701 tctcccattc ggtgagttcg gcgcgcacgc gcaggcgttg gccgtaacgc gccgaacgga
    14761 cgtacttgca acgcaggtcg acgactggcc agacgtagcc ggacgcgcgc atctgcggat
    14821 agtcgtagtc gatgccctgc agcagcgcgc agcgcgccag ctccaggtac ttgagcgtgt
    14881 gcccgtgcca gaccacgtcc atcggatcga ggtcgtggaa ggccgggctg agctcgatct
    14941 cggcgaccag cggcacggtc ggctcaatcg acatacaggc gccacgcctc gtcgcggatc
    15001 gcggccagca ggccatgcag gtcgcggtcg agcgcgcggt cttcctccac cagcgggatg
    15061 cgcgcttcca ggtcgaggaa catcgcctcg gcatcgtcgg acagcttggc gctgccttcg
    15121 tgggcacgac gcagggccat gccctggcgc gcggtcacca gcaatgcggc cacgacctgt
    15181 tcggtcagtt cgatcacccg caggcagtcg cgcgcggcga tggtgcccat gctgaccttg
    15241 tcctggttgt ggcattcggt gctgcgcgag aacaccgagg ccggcatcgt cagcttgagc
    15301 gcttccgcgg tccaggccga cgcgctgatc tgcagcgcct tcaagccgtg gttcagcgcc
    15361 gcgcgttcgc cgcgtgccgc cgacaggttt gccggcaggc cgtggttgta gcgcgcgtcc
    15421 accagcaggg ccatctgccg atccagcagg tcggcgaggt tcgccaccag gttcttcagc
    15481 gaatccatcg cgaaggcgat gtggccgccg tagaaatggc cgccgtgcaa caccatcccg
    15541 gcgtccgcat cgatgatcgg gttgtcgttg gcgctgttga gctcgttctc gatgttggtg
    15601 cgcagccacg gcagcgcatc ctgcagcacg ccgatgacat gcggtgcgca gcgcagcgaa
    15661 tagcggtcct gcaggcgctg ttcgttgcgc gacggccgtg ccgtggcgag gtccgcgcga
    15721 aggcgcccgg cgacgcgctg ctggccagga tgcggcttca ctgcgaacag ggcttcatcg
    15781 aaatggtggg cattgccggc gctggcgacc aggttgaagg cggtgatgcg cgtggcaagg
    15841 cggccgaggt agtcggcgcg ttgccacgcc aggcacgcca gcgcggtcat caccgcggtg
    15901 ccgttcatga tcgccaggcc ttccttgggg cgcagccgca acggctgcaa gccgtgcgca
    15961 gccaatgctt cgcctgccgg cttgacggcg ccgtcgtgca gcacctcgcg ctcgccgcag
    16021 agcacggcag cgacgtagga cagcggcgtc aggtcgccac tcgcgccgac cgaaccttcg
    16081 gccggaatgc gcggcaggat gtcgtgccgc agcagcgcct gcagttgccg cagcagcgcc
    16141 acgctgacgc ccgacgagcc ctgcgccagc gacaccaggc gcgccagcag caccgcgcgg
    16201 gtttcgtcgg catcgaggaa ccgccccagg ccgacgccgt gataggcgta gaggtgatgc
    16261 ggaagctcat gcaccagcgc cggcgggatc gcgacggtgc aggaatcgcc gtagccggtg
    16321 ctgacgccgt agaccacgcc gtcctcggcg atcaaccggt caacgaagtc ggcaccacgg
    16381 ccgatgcggg cgaggaagtc gcccgcgtcg gacagccgcg caacggccgt gccgcgggcg
    16441 acggcgacga catcctcgat cgtcaacgga tggcgcccat cgaccatgat cgaagcgggt
    16501 tcagcgggaa tggcgggcat tggcgtgcgg catccagaaa tcgtagaagt tgaaccagtc
    16561 gtacggcgat tgtcgcaggc agccggccag ccaacctgaa aaccgggcgg cgcagtcgtt
    16621 caggcccgcc tggcgatccg cgcgcggcaa ggtcacctgt tccgccagca gctcgatgcg
    16681 cgacgcgtag ccgtcgccat cgtgcaggca ggacagtccc cacaccgggc aacgcaacag
    16741 cgacgcgatc aaccacgggc ccgcggggaa atcggcctct gcaccgagga atggcgcacg
    16801 gcaggtgcgg tcgccgtgca ccggcacgcg gtcggcggcg atggcgatga attcgccggc
    16861 ggcgacgcga tcagccagcg ccatcgccat cgccggggaa aaatcggtga cctgcagcag
    16921 gcgcacggtg gcttgcgggt tcagtcgcgc caggatgcgg ttgaactgtt gcgcatgcgc
    16981 ggtgtgcacc aggatggtca cgcgcaggcc ctcgcgccag cgcgccgcca tctgcatgag
    17041 ctccaggcag cccatgtgcg cggtggcgat gacgccgccc tgccccgcct gcgcggcgtc
    17101 gagcatggcc tgctggccgc tgaagcggat ccgctcgcgc gggtagcgac cgccgatcgc
    17161 cagcatcttg tcgagcaggg tttcgccgaa caggaagaaa tgcgcgaggc tctggcgcat
    17221 cccgggttca cgccggaaca cgccttccgc ggcattcagt cgacgcaggt aatccagcga
    17281 tgcacgccgc gccggaccac tgcccagcca ccagaacaag accaccgggt acaggcagat
    17341 ccggaacggc aagcgcccga gcagccgata caccgcgtac aggaaccagg tgccgccgac
    17401 gaaggtggtt tcgccgaggt cggcccaatg cccgcggctc atcgcaccac cgtgcgcacg
    17461 cggcgaacga gcaggcgcgg tagccgccac agcatcccga agaacaggcg cgtgtgcatc
    17521 gccgaaatgc gcgcgttgtc gcgccacagg cggaaatgcg agacgccgtc catcgggtag
    17581 gtcacccgcg tcggcacggt acggatcggc acaccggccc agtgcaggcg gacgatgatc
    17641 tcgatatcga aatccatgcg cgtgccaacc gattcgcgat ccagcaaggc gagtgtcgcg
    17701 gcaagcggat agacgcggaa accgcacatc gaatcggcga tgtcgagcga cagcgtgttg
    17761 atccacaccc agacgtgcgt cgcgtagcgg ccgacgagcc ggccgagcgg cacgctctcg
    17821 tcgtagaccg gccggccatt gatgacggca tcgggatgcg cggcggattc ggccaggaag
    17881 cgcggcacgt catgggtgtc gtgctggccg tcggcatcga tctgcaggat atggctcgca
    17941 cccagcgtcg ccgcctggcg catgccggcc atcaccgccg cgccctttcc gccattgcgg
    18001 tcgagtcgca gcagcgacac ccgtccggca tggcggtcgg cgagttcgcg caacaccgcg
    18061 gcgcaggatg cgccggatcc atcatcgacc agcaggatcg gcacgccgtg cggcaacagc
    18121 tggtcaacgg tgtggccgat ggcatgctcg tgctcgtaca ccggcaccac gacccacggc
    18181 ctggatgtcg acgtcatggc gcctccccga agcgcagccg gccgctggcg tgcacgccac
    18241 gtgcggaggt gaagcggaag ccgagctgtc ccggccgcca gtccagctcg gcggtcagcg
    18301 ccgtgcccgg acgcaccagt tgctggaact tcaaggcttc catgcactgc atgtcgccgg
    18361 ccacgccgaa ggcgtcgcgg ccgaggcgca ccgcccaatc caccagcacc acgcctggca
    18421 ggacgtggat atgcgggaaa tggccttcga acgccgcgag ctcggtgccg gccagcaagg
    18481 tcgcggtggc cttggcgtgc tcgcgcagct gccagtcggg cgtcggcatc aacggccgga
    18541 acagcgccgc aaggtcgcgc tcgcgggtct tgccgcgtgc atccgccggc agcgcatcca
    18601 ggaagcgcca gcgccgcggc aaggcaatgg gatcgcaatg gccggccagc catgcgcgca
    18661 ggcgttcgac gagtgccggg cgaccctcgc catcgagcaa cgcctgccct ttcatgctgg
    18721 cgaccgctgc aaccgccagc aggatgcgat ggccagggag tgcaagcacg cggacgtcat
    18781 ccagcaagtc gctgtcctga agccgcgcct ggatcgcgtc cagggaaata cggcgttctt
    18841 ccagtttgac gatgcggtcg gcgcggccaa gcagttcgaa acgaccagcg cctgcatcgt
    18901 gcgcacgatc gctggtcgac caccagtcgt tcgatggcag gtgcggcgaa cgcacgtcga
    18961 gcaagccgtc ctgcacgcgc cattgcacgc ccggcaaggg ctgccacggc gcgcggctgc
    19021 catccccgcg tcgccaggcg acgccgccgg tttcggtgct gccgaagact tcgatcgccg
    19081 cctgccccca caggcggtgc acggtcagcg ccgcatccac cggcaacggt ccgcccgacg
    19141 agaacaccac gcgcaacgcg gacgccagtg cctgccaatc ctgcgtgtcc ggcaaccgct
    19201 tgagatgcgc gggactggcg accagggcca ccggaccggg acccagcgtg gtcagctgtt
    19261 cgttgtaggc gatgcgtgcc ggcgcgaacg cgcgtgccga cgacagcggc cacaggatgc
    19321 ggaacagcag gccgtagatg tgctggtgcg agaccgtgcc gtggatcacc gcatctgcat
    19381 cgatcaacgg tccgaacacg gattcgaggg cctcgacttc cgcatccaac tggcgcagcg
    19441 acttgcggat cgccgcgggt tcgcccgtcg agcccgaggt gaacagcaca agcgtgcagg
    19501 tgtccgcatc gagtgcaccc agccttgcat cgatcgaccc gtgtgaccgg attgcgcaga
    19561 tgccacccgg caagtcgccc gcgaagatca atccgcgttc gcgcaagttg tccagcgtcg
    19621 ccggcaaggt atcgcccggc aaccacggcg tgacgccagc ctgccatgcg ccgaacaagg
    19681 cggcggaaaa ctcaagcgcg tcatcgcagg ccagggcaac gtcgttgcct gcatgcgcag
    19741 tgagtgcttc gcgccatgca ccgacgcgca ggcgccactg cgcgtggtcg atgccgggcg
    19801 ataccgatgc cgtggcatcg acactgcaga gtcgtggcaa gggaatgcgc atgcgatcaa
    19861 ccggcatggc gcggcctgag cagccactcg ccggccagca tcgcgccgat gagcacgtag
    19921 gcaatcgcgc cgttgtacag cgcccatgtg gcatcggacg ctgccagtgc cgtccaaagc
    19981 gcgatgccac cgttggccac gaagaacacg caccacgcac gcgtcacgtt cgcgacccag
    20041 cgcacgcctt ccggcggcag gtcgggatgg cgcaaacggg ccaggcgttc gatcacggtc
    20101 ggcggcttcc acaggctggc ggcgaacacc agcagcatcg ccacgttcac cagcaccggg
    20161 tagagcttga gcggcaggcc gtcattcagg acgaaggtgg cgatcaccag cacgagcgcg
    20221 ccggacgcgg cgagccacca gaccggttca cgcgaggcga gcgcgcggag cgatgccacc
    20281 agcagcagga acaggcccag ccagcggggt tcgaaacgcg ccagcccgag ccagatcgcc
    20341 agcggataca gcagggtcgc cagccacgcc agggccgtcg cgacgcgcac gggttacttg
    20401 accgtggcga cgttgcccgc atgcagcatc gtgtggacgg tatcgaccac gtcctgcacg
    20461 ctgcgcacgg ccttgaatgc ctccgggcgc aggttgccgc ccagcatcgg cttgagctgc
    20521 acgatcaggt cgaccgcatc gatgctgtcg atgtccaggt cgccggcgag catggcctgc
    20581 ggagtgatgc tcgcccgttc gatcccgaag gtgtcgtgga ggatgccggc gatgcgctgg
    20641 aagatctcgt ccttgtccat cgtgctgtcc gtgcctgtca ctgcgagcga ctggcggcga
    20701 cgaatgcggc caggctgcgc acgctggcga agtgccggcg ggtttcctcg gaatcggcgg
    20761 ccaaggtcac gccatagcgc ttctgcaggg ccagcccgag ctcgagcgcg tcgatcgaat
    20821 ccagccccag gccctcgttg aaaagcggcg cttcggtgtc gatgtcgtcg gcgacgacgt
    20881 cctccagcga catcgtggag acgatcagcg ccttgatgtc ctgttccagc gaattagtgt
    20941 ccagcgcctg cacgcagcag ttctcccgtg aagatgtccc cgagcgcagc cgtcaggcgg
    21001 cgtgccgcca atggctcggt gacgccgtct tcgagaaacg gtgagaccgg caggtctggc
    21061 aggacgtcca tgcacagatg gaagcgccgg ggcggcacac gataccattt ctcccccttc
    21121 cccagcgtgc gcggctcgca gcggatcacc accggcgtca cgtccaggcg cccgcgcacc
    21181 gcgatgttcg cggcaccgcg ttgcaagggt cccagccgtt cgccagccgg cgtgcgcgtg
    21241 ccttccggga agatcagcag gttgcgccct gaacgcaccg aggcgatgca gtcgtcgacc
    21301 aggccggcgc cgttgtcgtt gcagatgtag ccggtcgcgc gcaccggacc gcgggtgaac
    21361 ggattgttgg ccaggcgcga cttcaccacg caatccgcgt ccggcaggcg cgacaccagc
    21421 aggaccacgt cgaccagggt cggatgattg gccagcacca gcaggccccg gcgttgcagg
    21481 cgctccaggc cgcggatctc gaaggtcatc acgcccagcc catgcatgac ccggatgtgc
    21541 ccggcgaaac tggcgcggat caaagcgcgt gcgcggcgct ccagcgtcgc ccggtcgcgg
    21601 ctgaacaggc cgaccagcgg gaacaccagc acccgcaaca gcagaccgcc cacgccgaac
    21661 gcgatgaacg acaacgcggt gccgaaggtc cgccacgcgc ggtcgaatgc atcagccatg
    21721 ccggctccag ccgccaccct cgacgcccgg caaggacgca tcgccggcca ggaagaagcg
    21781 cagcacgtcc agcgaatgcg gcagcacgcc ggatcccgca ggttcgcccg cgcggagcga
    21841 aatacgcggc aagccatctc gcggcgcgac gatccgccag gcccaggcgt gcagcgcgtc
    21901 gggctcgtcg aggaacacgt cgtagtcgcc tggcaccgca tcgtcgtaca ccacgaccag
    21961 caccgcgggc gcgccatccg ccagcaagcc gagcgcctcg gtgaccgctg cctccggcgt
    22021 ggcacgcacc gccgataccg cgaggtagtt cccctgcagc aggcgcgcga tgctgtattg
    22081 cgcggcgatc gcgttgtgcg tcgacaggcc gaactgcgtc ggcgacaacg gttcgtcacg
    22141 cgcaagttcg gcgagcatgc ggtgcgtgcg atccaggtcg ccatgccggc tggcgaacac
    22201 cagcggcagg tcgcggtcgt cgtcatgctg gcaacgccac gcgacctgca acgcggcgcg
    22261 ccccaggcgt tcgacgcgac gccgctgcat cggcgccatc tccgccaacg ccggcatcgc
    22321 ctcgccggtc ggcagccagg gcgcgcgcgc ccatgcgtgc cagtctgcgt ggctttcgag
    22381 ccctgcggcg aacgccgacc aggcatcgat gctgaacgtg gcatccgtgt cgattgcgtg
    22441 ctccatgtgg cgtgttgccg cttgcgtggc gctgcatgca tcttccgcgc tggcgccgca
    22501 aagaaaaagc ccggcgtgag ccgggctttc gatgaagcac ctcgatttcg cggttcgtgg
    22561 tggcgtcccc acggggattc gaaccccggt cgctaccgtg aaagggtaat gtcctaggcc
    22621 tctagacgat ggggac
//
LOCUS       DAOOCX010000031        22519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_1979_length_22519_cov_9.015491, whole genome shotgun sequence.
ACCESSION   DAOOCX010000031 DAOOCX010000000
VERSION     DAOOCX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 22519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22519
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1979_length_22519_cov_9.015491"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..534
                     /locus_tag="PK399_04680"
     CDS             7..534
                     /locus_tag="PK399_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPW12450.1"
                     /translation="MTDPSQAPDAPPSSAQPKHRQLKLENFLPYRLSVLSNRTSNAIA
                     REYSERFDMTIPEWRVMAVLGRFSGLSANQVAQRTAMDKVAVSRAVAKLMDAGRIARE
                     FDDDDRRRSVLRLSEAGYAVYDQIVPLALGFEQHLLGDIPADERALLFRLLDRLDELE
                     LRAEHYNGGADTNPG"
     gene            complement(604..2136)
                     /locus_tag="PK399_04685"
     CDS             complement(604..2136)
                     /locus_tag="PK399_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HPW12451.1"
                     /translation="MSGAATTTTPANPPEFPQVLGHPRPLWMLFMSEFWERFAFYGIR
                     WAMVLYIVAQFHGGDASGEKPASELYGAYLALVYAGAIFGGYVADKLIGFQRSILLGA
                     VIMATGLFLLASPSEAMFKLGLATIIVGNGLFKPIISTMVGKLYAQGDDRRDSGFTIF
                     YMGINIGALLAPLITQYLAKKIFGIEDTPAYKIVFIAAGIGMLLSLVWFWFGRTQLKG
                     IGLPAAHQTGMRNVSLVLLFGVVIGIPMFYALLTIDANLLQAILMALFVIPAVMLFRE
                     GVVEGKVSRDKVMAMLTIFVFNVLFWMFFEQAGSSFNFLAEKIVDRDIGMVGMFQALA
                     GTNDFPVSWFQSVNSVAIIALAPVLAWLWVKMGRNNPIIPRKFGLGLIFNGLAFLLLM
                     VALSGMVDENGKIPFWTLFMVYVIQSVGELCLSPIGLSMTSKLAPARLGGLAMGCWFL
                     SIAIGNNLAGIFAGKVSGEGGMTVESAHAGYTFGFYALVGSGVLLFLIAPLIQKWMHG
                     VK"
     gene            complement(2275..3165)
                     /locus_tag="PK399_04690"
     CDS             complement(2275..3165)
                     /locus_tag="PK399_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HPW12452.1"
                     /translation="MSIDRNERPLEAGIHTDLEGRLTYGGYLRLDQLLSAQQPLSQPP
                     HHDEMLFIVQHQTSELWLKLLIHELTAATDHLRNDRVWQFGKVVARCKRVLDQLTAQW
                     SVLETLTPSEYMEFRDILGPSSGFQSLQYRMVEFLLGNKNAAMLKVFAHDVDGHAALQ
                     AALVAPSLYDEFLRYLGRWGHDIPWQRQERDFTQPHVMDPALVPVFERIYEDTGRYWR
                     EYALCEDLVDLETQFQLWRFRHMRTVMRIIGFKPGTGGSSGVAFLRKALELTFFPELF
                     EVRTAIGPTRDYGAPDLPTR"
     gene            3278..6550
                     /locus_tag="PK399_04695"
     CDS             3278..6550
                     /locus_tag="PK399_04695"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPW12453.1"
                     /translation="MKHLIQRGALMALALSASFAAQAQANLTGGTYSQDFDTLSNTAG
                     STTNSTLPAGWLLTETGGGARDNEQYAVDTGSGNTGDTFSYGAAGATDRAFGGLQSGT
                     LIPVFGACFTNGTGSALSSFDVAYTGEQWRLGTAARADRIDFQYSLNATSLTTGAWVD
                     VDALDFATPNTATTGAKDGNAAGNRSAIAATVSDISIASGATFCVRWNDFNASGADDG
                     LAVDDFSITVAGTPLPGLSINDVTQVEGNSGTTTYSFTVSLSAPAPVGGVSFDIATSS
                     DSATSGVDFVAKSLTGQTIPQGASTYTFDVLVNGDTALEINETFLVALSNATGANIAD
                     ASGTGTITNDDIVITSIGTIQGSGELSTYDGQLVMTEGVVTAKRGNGFFLQSFAASGD
                     AAIDGNAATSDGIFVFTSAAPSMVTVGDNARVTGTVDEFLGSAATPTTEIVSPSVLVL
                     GNGIPLPAPVEITAALAGADSLPDSLERLEGMLVRVAQGVVTAQTDGNINEASATSSD
                     SNSAFEFVVTGVSRPLREPGISILDPFPVPPAKQATIPYFDANQERIKAFPLVGTRLV
                     ADAGAPVSNLVGVLTYFGDSWELLYDVANPPVIGSATATAVTTPVADDITVAGFNLLR
                     FFDTTNDPAIGEPVLTPSAYANRLAKTGAAICDWVKAPDILGVVEVENIGTLTDLANY
                     INGNCASAPAYVPYLQEGNDVGGIDVGFLVSNRAVRAGVSRVAVGSVTQVGKTTLYGT
                     NPLGCTPGAPGCTSSLLNDRPSLVLRATVNFADGRMYPLTVIANHLRSLNGNDDPADN
                     RVRFKRAEQAMELGRLVNGMQVANPNEKIVLVGDFNAFPFNDGYVDSMGIITGNAAPE
                     PEVIEYRASPVATPLVLGDALTADPAQRYSYVFEGNAQTLDHAVVNAALVNDPAVAGL
                     SVEHARINADFRVFHYGQFGLPYTAQNPPLRVSDHDPVRLSIRVVRPASADLALAWST
                     RSMTSKGAFATLTLRNTGASPISGAQVRITLDIPADATAAVGPPSGWTCTRPADAFVG
                     DTEFLCTASTSVPVGSSQGFSVTVKPRRSFAASDVLRFEARATVLTDPISGDNSAVLV
                     LP"
     gene            6671..7744
                     /gene="pdhA"
                     /locus_tag="PK399_04700"
     CDS             6671..7744
                     /gene="pdhA"
                     /locus_tag="PK399_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016850583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HPW12454.1"
                     /translation="MTVAARFEIEYLQYLKPDGTLAGDLPPAVPDANALLPLFKRMLF
                     VRTFDSKAIALQRTGKLGTYAACLGHEATHVGIGASMQPDDVFAPSYREYGAQFERGV
                     KPRDVLMYWGGDERGNDFEIPRKDYPWCVPISTQCLMASGAALSFKLRGENRVAVATC
                     GDGGSSKTDFYAAVNSAGAFTLPLVLCVINNGWAISVPRKAQTGADTLAQKGLAGGLH
                     CLQVDGNDLVAVLEAMRRAHERARSGQGGSVIEFMTYRLHDHTTADDARRYRGEDEVK
                     AAWEKEPFLRLRKYLTDQGLWDESQEKAWIEECGKLVDVEINAYLETPVQPVEAMFDY
                     LYGDMPADVLAQREQAIALEKRA"
     gene            7741..8727
                     /locus_tag="PK399_04705"
     CDS             7741..8727
                     /locus_tag="PK399_04705"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HPW12455.1"
                     /translation="MSEQPIALIEAITQALAYELRNDPSVVVLGEDVGVNGGVFRATA
                     GLSATFGTERILDTPLDETTIAGLTVGMASQGMKPVAEAQFDGFMYPMVDFIVCHAAR
                     MRYRTRGRLTCPMVLRVPWGGGIRAPEHHSEANESIFTNVPGLRVVMPSSPQRAYGLL
                     LAAIRDPDPVIYMEPKRIYRQYKELVPDDGEALPLDVCYVLRDGSDVTLVTWGAQVKE
                     TLEAAEKLAADGISAEVIDVATLKPLDFATIAESIAKTGRCVIVHEAAKTAGFGAEIA
                     ARCAEECMFDLVAPVERVTGYDTHIPLFRLEMKYLPSTDKVIAAARRAMAHS"
     gene            8737..>9971
                     /locus_tag="PK399_04710"
     CDS             8737..>9971
                     /locus_tag="PK399_04710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPW12456.1"
                     /translation="MSKKFMLPDLGEGLPDATIVEWYVKVGDTIKLDDNLVSMETAKA
                     VVDVPSPVSGKVLQLAGAAGDIVVTGTMLAEFEVDASMPQRAEGQDTGHHHGPTHEEP
                     KPASAPEPAAKADAGTVVGAMQVGDAVVAQAAIAAGGVKAMPAVRATARKLGVDIGRV
                     AATGPDGTVTMDDVKRAAADGSAKPGAAPARSGAQAAVAQVAAPAVASRSTLSQSGKP
                     MRTQPPGAIASGQPEQLKGVRRNMARVMADAHAQVVPTTLVDDADLHAWSGRQDITAR
                     LVRAIVTACKTVPALNAWFDGANLTRTLHPHVDLGIAVDTDDGLFVPALRNADMLDGN
                     GVRAAIKRLRAQVEERSIPSSELSGYTISLSNFGMFAGRYATPVVVPPCVAIIGAGKL
                     SHDVVAVMGGIEVHRRMPIS"
     assembly_gap    9972..10071
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10072..10403
                     /locus_tag="PK399_04715"
     CDS             <10072..10403
                     /locus_tag="PK399_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW12457.1"
                     /translation="DAATDFWSQALGCTIADRDAGDGDAEYQMLGDTPGDLHIEVQKV
                     AHPSRVHLDIETDDVDAEADRLEALGAKKIAFVKRWWVMEAPTGQRFCIVRTKFPERG
                     VAWNDWD"
     gene            complement(10404..10697)
                     /locus_tag="PK399_04720"
     CDS             complement(10404..10697)
                     /locus_tag="PK399_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12458.1"
                     /translation="MPALALILFAPWFLILATLYWLYPRQPRNAARLRFDAIALLLAF
                     AGCLASLYWSFGFADRSHGHLWPQILATSVGYGVFLAVLTGAFYLRRRMLATG"
     gene            complement(10709..12031)
                     /locus_tag="PK399_04725"
     CDS             complement(10709..12031)
                     /locus_tag="PK399_04725"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPW12459.1"
                     /translation="MSHTRVLTGITTSGTPHLGNYVGAIRPAIAASHAPGVESFYFLA
                     DYHSLIKAQDPARTQRSTLEIAAAWLACGLDPDKVWFYRQSDIPEIPELTWLLTCVAG
                     KGILNRAHAYKAAVDRNRAEGEDDDAGVSAGLFMYPVLMAADILIFNAHKVPVGRDQV
                     QHIEMARDFGQRFNHIYGQDFFVLPEAAVDDNVATLPGLDGRKMSKSYDNTIPMFAPR
                     GELKKLVAGILTDSRAPGEPKDTEGSALFQLYQAFASTEDTAAFAQAFADGIGWGDAK
                     QTLFERIDAEVTPLREKYEALMARPGEIEAILRDGASRLRAEHATPTLRRLREAVGLR
                     DLSQVSTVDAKSTKKEASATVPTFKQYRDADGKFYFKLVQGERVLLQSTGFASPRDAG
                     QRIAAIKQGEIGDGASDFALGEGVGDEEVNAALAAFVAAQLEGEAGKA"
     gene            12154..12957
                     /locus_tag="PK399_04730"
     CDS             12154..12957
                     /locus_tag="PK399_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12460.1"
                     /translation="MFRRLLLALSLAASVACSTPVSAGDLVQLDVVDRDNGQVLPEYR
                     HRGQDWIAGVPGHRYSVRLTNTTGERVLVVLSVDGVNAVTGQTASPSQGGYVLEPWET
                     AEIAGWRKSLDDIAQFVFTDLPDSYAARTGRPANVGVVGVAVFRERQVQPAYAPPPAP
                     PIASGMARNESRKASAPAASADRAMASEAEAMPQRLGTGHGQREWAPVGQTEFVRASR
                     SPQQVSQLRYDDADALVALGILPHYRAPYAGSTPRAFPNGFVQDPPYRY"
     gene            complement(13035..13139)
                     /locus_tag="PK399_04735"
     CDS             complement(13035..13139)
                     /locus_tag="PK399_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HPW12461.1"
                     /translation="MLLCMLVLAGCNTIAGAGKDVQKVGEKVEEAATK"
     gene            complement(13330..13482)
                     /locus_tag="PK399_04740"
     CDS             complement(13330..13482)
                     /locus_tag="PK399_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010374875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HPW12462.1"
                     /translation="MKRLFALLLLAMFSMSTLTACNTMAGAGKDVQKVGEKVEDKAQD
                     CKDGKC"
     gene            complement(13629..14552)
                     /gene="rocF"
                     /locus_tag="PK399_04745"
     CDS             complement(13629..14552)
                     /gene="rocF"
                     /locus_tag="PK399_04745"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HPW12463.1"
                     /translation="MTRTTPPVSVFGVPTDIGASRRGASMGPEALRVAGLVEAIAARG
                     IDVRDIGDVQGPRNPTSPPVDGYRHLDEVVAWNRAVFDASTRELQAGRMPIMLGGDHC
                     LGIGAIAAVAAHCRATDKKLRVLWLDAHADFNTREITPSGNIHGMPVACLCGLGPDVL
                     TGMAGFTPAVTPDQIRQIGIRSVDPGEKRLVQQHGLDIYDMRYIDEVGMRRAIEEALE
                     GVDADTHLHVSFDVDMLDPSIAPGTGTRVPGGVNYREAQLIMEMVADTGCMGSLDLVE
                     VNPALDKRNATARLAVDLVESLFGKSTLMRD"
     gene            complement(14613..14688)
                     /locus_tag="PK399_04750"
     tRNA            complement(14613..14688)
                     /locus_tag="PK399_04750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14653..14655),aa:Thr,seq:tgt)
     gene            complement(14735..15439)
                     /locus_tag="PK399_04755"
     CDS             complement(14735..15439)
                     /locus_tag="PK399_04755"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPW12464.1"
                     /translation="MLLRATLVILFMLNLGAAAWWGLRPSVADARGAEANAAGAPGLR
                     LVNEPAVATAPTRAQPAPAGPAPVASMVPAATSVVAAPASAPVCLRFGPFADGTARDA
                     ARNALMAAGVSAVTRDSPARSARGWKVAMPPLASREDAVAMAERIKAAGISDLYVMNE
                     GADANSIALGRFGSEDAARRREAELRGKGFQAQAMPLGDTPSQAWLDARLPTATDPAS
                     LAAIAASRAIDCATLR"
     gene            complement(15449..16168)
                     /locus_tag="PK399_04760"
     CDS             complement(15449..16168)
                     /locus_tag="PK399_04760"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPW12465.1"
                     /translation="MRTWLFDLGNTRLKCAQLHADGSIDEPLAIAHDDGTAWLDALPS
                     GSVACIASVASDVRRAALFDALCARFTRLHRVRTERVLGPLRIAYDNPAHLGVDRFLS
                     MLGQCGEGASLVVGVGTALTLDLLDGGGLHRGGRIAPSPRTMREALHARAAQLPVEGG
                     IYAEFADDTGDALASGCEGAALALVERSLGAATALLGESPAVCLHGGGADLLRDRLPG
                     HRWQPDAVLRGLARWHALRIA"
     gene            complement(16165..17160)
                     /gene="birA"
                     /locus_tag="PK399_04765"
     CDS             complement(16165..17160)
                     /gene="birA"
                     /locus_tag="PK399_04765"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004136871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HPW12466.1"
                     /translation="MRAGDAQQERGLLQRLAAGPVSGDTLAREAGLTRAAVWKRIDAL
                     REAGIDIGASPGRGYALRQPLDLLDADAIRAGLPASVQGGLVDLEVAWSLDSTNSELL
                     RRDTPAHGCAVLLAERQTGGRGRRGRAWASPLAAHVYLSLSRRFEGGLARLGGLSLVA
                     GVAACDALHGLGFDGVRLKWPNDLVLVDGAGLRKLGGLLVEGGGETAGAARAVIGIGL
                     NMRMPARDGAGIDQPWAELSGLSARPPSRNVVVTALLAHLLPALDAFDAQGLSPFLSR
                     YAGFDALAGREVAIHDTTSAWHATALGIADDGALRVRDASGSERRVHAGDVSVRL"
     gene            complement(17164..17814)
                     /gene="plsY"
                     /locus_tag="PK399_04770"
     CDS             complement(17164..17814)
                     /gene="plsY"
                     /locus_tag="PK399_04770"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPW12467.1"
                     /translation="MPPILLTALLLLLAYLLGSVSGSLVLGRFRNIDIREHGSGNAGG
                     TNALRTLGWKFALGVAVIDVAKGALATWLVLRYAPVGTGLSVTAHGYLAAALAVVGHV
                     WPLWHGFRGGKGAATGVGGLLVLWPWIVPLLLLAWILVLVSTGYVGLSTVVAALCLPL
                     LAWGFDAGPERMWFCIGMGLFLAWTHRANLVRLWHGKESRFERARLLYRLYGRSRY"
     gene            complement(17843..19660)
                     /locus_tag="PK399_04775"
     CDS             complement(17843..19660)
                     /locus_tag="PK399_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPW12468.1"
                     /translation="MPVATADPPAVTAPALRRALIAGARRVIAARDDLNRINVFPVAD
                     GDTGSNLASTLGSVLQGALSRRSRHIGELLVGIGNDAIDGARGNSGAIMAQFLVGLAE
                     QARNAPVLDAATLALAVRRGADSARAALAQPVEGTILSVISAFADALDEAAKQPQGDP
                     KGGFARALQRARTALADTPKQMALLQKAGVVDAGARGFVDWLEGIAEYVDGGPRAVRL
                     HGHATAAANDAGPMPAHVHEDVDPAHRYCTECLVTGDGIDRVALQAALAGAGVDSLVV
                     AGSATRVRVHGHVGTPQRLFDACAGFGHVDAMKADDMVLQQRSTESPSLLAVITDSAS
                     DLPAVIAERYGIHVVPARISLDGRDYLDRLGLTAGEFYRRMAASKALPQSSQPPPGDF
                     RRVFDHVLAYQPGAVYVGVSRTLSGTLQSGETAARGRDKPVRCIDSGHVSAGESLLAW
                     RAGEMAAAGADGDAIERELQRLRPLTKTWAMARDISHAVRGGRIPRWAGPVVRWSGLT
                     PVAAVSDAGTLKVAGGLFAKQRAPEAFARYVARRTPASKGWRVVVGHSDALADGERLL
                     AALRERLPVVEAHLVEVGPAVGAHAGQGTLVVGLQPSPD"
     gene            19799..20083
                     /locus_tag="PK399_04780"
     CDS             19799..20083
                     /locus_tag="PK399_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12469.1"
                     /translation="MRLRPMLLCLALLAASGHAMATESRQSASDEACPDTTAASASER
                     TDDGQPAEEPTAPTRQTHKAKPAVSASPRATGGTRTSPPRFHSFLPGMFR"
     gene            20112..20867
                     /locus_tag="PK399_04785"
     CDS             20112..20867
                     /locus_tag="PK399_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HPW12470.1"
                     /translation="MIWPFSRRKATLPLPLLDDTLWRQAAAGLPWLRRIDADRGQRLR
                     QLVTEFLRDKAITPLQGLRLNEVQRLRLAMLCCLPLLEFGREGLHGWSQLLVYPQAFR
                     VQRNHMDAQGVLHEWDDELIGEAWERGPVILSWADVRADIATPDDGFCVVAHEIAHKL
                     DALDGAMDGTPPLPRDWQRQWASDFQRAYEGLCNAVDAGRETTIDPYAAESPEEFFAV
                     CTEHHFSDSATLRKAMPDVAAHLQRFYGPPLLA"
     gene            complement(20902..22305)
                     /locus_tag="PK399_04790"
     CDS             complement(20902..22305)
                     /locus_tag="PK399_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005989362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPW12471.1"
                     /translation="MRRLRIAQPRSLQSRQLWAASLGLIAFLALAGFALDRAFQSTAQ
                     SGMRERLHSYALAYANSDFARDGSVIPPYDPPDPRFRRPGSGLYAQILLPSGQWQSDS
                     AQGPRLPEGAMLDAGQRVFEGPLPLTRIDGGAGQVYRYGYGLLWTGSEDSRREFPYTV
                     YILEDTTALGRQVAAFRSALWRYLGGAGVVLLLLQMLVLRWSLRPLRDVIAELKRVQH
                     GQAAGMSEAHPRELEPLTDSINAFIEGERENLDRQRNTLADLAHSLKTPLAVLRTRLD
                     SGAGESELRDEVETQLRRMNDLVGYQLARAARSGHQLFAAPIEIEPQAEQIVTGLEKV
                     YAAKRVLCEFEIAAEARFHGETGDLQELLGNLLENAFKWARSKVLLTVAEGTPVPGRR
                     PGLSITVEDDGPGIADDRIAHVLQRGVRGDERVQGHGIGLAIVQDIVKAYRGELHVGR
                     SQELGGAKFEVVLPPGL"
     gene            complement(22382..>22519)
                     /locus_tag="PK399_04795"
     CDS             complement(22382..>22519)
                     /locus_tag="PK399_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPW12472.1"
                     /translation="FDRDSNVLEVFIGRLRKKLDPDGALKPIETVRGRGYRFAIARSG
                     D"
BASE COUNT         3741 a         7970 c         7394 g         3314 t
ORIGIN      
        1 cagcacatga ccgatcccag ccaggcacca gacgccccgc cctccagcgc ccagccgaag
       61 caccggcagc tgaagctcga gaacttcctg ccgtaccggc tcagcgtgct gtccaaccgc
      121 accagcaatg cgatcgcccg cgaatattcc gagcgcttcg acatgaccat cccggaatgg
      181 cgggtgatgg cggtactggg tcgcttctcc gggctgtccg ccaaccaggt cgcgcagcgc
      241 acggcgatgg acaaggtcgc ggtgagccgc gcggtggcga agctgatgga tgccggacgc
      301 atcgcccgcg agttcgacga cgacgaccgc cggcgctcgg tgctgcgcct gtctgaggcc
      361 ggttacgccg tctacgacca gatcgtgccg ctggcgctgg gcttcgagca gcacctgctg
      421 ggcgatatcc cggcggacga acgcgccctg ctgttccgcc tgctggaccg cctggatgaa
      481 ctggaactgc gcgccgaaca ctacaacggc ggtgccgata caaaccccgg atgaccgcaa
      541 gacgcaacgc aaacgaaaac ggcagccgag gctgccgttt tcgtttcgtg cgttggagga
      601 tgcttacttc accccgtgca tccacttctg gatcaacggc gcgatcagga acagcagcac
      661 gcccgaaccg accagcgcat agaagccaaa cgtatagccg gcgtgggccg actcgacggt
      721 catgccgccc tcgccgctca ccttgcccgc gaaaatgccg gccaggttgt tgccgatcgc
      781 gatcgagagg aaccagcaac ccatcgccag cccgcccagg cgcgccgggg cgagcttgct
      841 ggtcatcgac aggccgatcg gcgacaggca cagctcgccc acggactgga tgacatagac
      901 catgaacagg gtccagaacg ggatcttgcc gttctcgtcg accatgcccg acagcgcgac
      961 catcagcagc aggaaggcaa ggccattgaa gatcaggccc aagccgaact tgcgcgggat
     1021 gatcgggttg ttgcggccca tcttcaccca caaccatgcc agcacgggcg caagcgcgat
     1081 gatggccacc gagttcaccg actggaacca ggacacgggg aagtcgttgg tgcccgccag
     1141 cgcctggaac atcccgacca tcccgatgtc gcgatccacg atcttctcgg ccaggaagtt
     1201 gaacgagctc ccggcctgct cgaagaacat ccagaacagc acgttgaaca cgaagatggt
     1261 cagcatcgcc atcaccttgt cgcgcgacac cttgccttcg accacgccct cgcgaaagag
     1321 catgaccgcg ggaatgacga acagcgccat caggatcgcc tgcagcaggt tggcgtcgat
     1381 ggtcaggagg gcatagaaca tcgggatccc gatgaccacg ccgaacaaca ggaccaggct
     1441 gacgttgcgc atgccggtct ggtgcgctgc gggcaagcca atgcccttca gttgcgtgcg
     1501 cccgaaccag aaccagacca ggctcaacaa catgccgatg ccggccgcga tgaacacgat
     1561 cttgtaggcc ggcgtgtcct cgatcccgaa gatcttcttc gccaggtatt gggtgatcag
     1621 cggcgccagc aacgcaccga tgttgatgcc catgtagaag atggtgaagc ccgaatcccg
     1681 gcggtcatcg ccctgcgcgt aaagcttgcc caccatcgtc gagatgatcg gcttgaacag
     1741 gccattgccg acgatgatcg tggccaggcc gagcttgaac atcgcttcgc tgggcgacgc
     1801 caacaggaac aggccggtgg ccatgatcac cgcgccgagc aggatcgaac gctggaagcc
     1861 gatcagcttg tcagccacgt acccaccgaa gatcgcgccc gcatagacca gcgccaggta
     1921 cgcgccgtac agctcgctgg ccggcttctc gccggaggcg tcgcccccgt ggaactgggc
     1981 cacgatgtag agcaccatcg cccagcggat gccgtagaac gcgaagcgct cccagaattc
     2041 cgacatgaac aacatccaca aggggcgcgg atgcccgagt acctgcggga attccggcgg
     2101 attcgccggc gtggtcgtgg ttgcggctcc gctcatgcgg tctccccctg aagtcgttgt
     2161 tttgattgtg gcgtcacggt ccgcggcggg cgcggaaccg ggcgaggatg gccgaggagc
     2221 gcgggcaacg tcaaaccgcg cccattcagg acgcggcgtg caacgaccgc aggctcagcg
     2281 cgtcggcagg tccggcgcgc cgtagtcgcg ggtcgggccg atcgccgtgc ggacctcgaa
     2341 taattccggg aagaaggtca gctccagcgc cttgcgcagg aaggccaccc cactggaacc
     2401 gccggtgccc ggcttgaagc cgatgatgcg catgaccgtg cgcatgtgcc ggaaccgcca
     2461 tagctggaac tgggtttcca ggtccaccag gtcctcgcac agggcgtact cgcgccagta
     2521 gcggccggtg tcctcgtaga tccgctcgaa caccggcacc agggccggat ccatgacgtg
     2581 cggctgggtg aaatcgcgtt cctggcgttg ccaggggatg tcgtggcccc agcggcccag
     2641 gtagcgcagg aactcgtcgt agaggctcgg cgcgaccaag gcggcctgca gcgcggcgtg
     2701 gccatcgaca tcgtgggcga acaccttgag catcgccgca ttcttgttgc ccagcaggaa
     2761 ctcgaccatc cggtactgca gcgactggaa ccccgacgac ggccccagga tgtcgcggaa
     2821 ctccatgtat tccgaaggcg tcagcgtctc cagcaccgac cattgcgcgg tcagctggtc
     2881 gagcacccgc ttgcagcgcg cgaccacctt gccgaactgc cagacccggt cgttgcgcag
     2941 gtggtcggtc gccgcggtca gctcgtggat cagcagcttc agccacagct cgctggtctg
     3001 gtgctggacg atgaacagca tctcgtcgtg gtgcggcggc tgcgacaagg gctgctgcgc
     3061 ggacagcagc tggtccaggc gcaggtagcc accgtaggtc aggcggccct cgaggtcggt
     3121 gtggatgccg gcttccagcg ggcgctcgtt gcggtcgatg ctcatgggcc gattctcgca
     3181 tggcccccac cgatgccatg cagctgtcac ggaaatgcag caaaatcggc ccgtgcgaac
     3241 cgggggccgg ttcgcggcaa ccctggggaa ccccgcaatg aagcacctca tccagcgcgg
     3301 cgcgctgatg gccttggcct tgtcggcgtc gttcgccgca caggcacagg ccaacctgac
     3361 cggcggcacc tacagccagg acttcgacac attgtcgaac accgctggca gcaccacgaa
     3421 ttccacgctg ccagcgggct ggctgctcac cgaaaccggt ggcggtgcgc gcgacaacga
     3481 gcagtacgcc gtcgataccg gctccggcaa taccggcgat accttcagtt atggcgccgc
     3541 cggcgctacc gatcgcgcat tcggtggcct ccagagtggc accctcatcc cggtgttcgg
     3601 tgcctgcttc accaacggca ccggtagcgc cctgagcagc ttcgacgtcg cctacacggg
     3661 cgaacagtgg cggcttggca ccgcagcgcg cgcggaccgg atcgatttcc agtacagcct
     3721 gaatgcgacg tcgctgacca ccggcgcgtg ggtcgacgtc gacgcgctgg acttcgccac
     3781 gccgaacacc gccacgaccg gtgccaagga cggcaacgcc gcaggcaacc gcagcgccat
     3841 cgccgccacc gtcagcgaca tatcgatcgc cagtggtgca acgttctgcg tgcgctggaa
     3901 cgatttcaac gcctcgggcg cggatgacgg cctggccgtc gacgacttct ccatcaccgt
     3961 cgcaggcacc ccgctgccgg gcctgtcgat caacgatgtc acccaggtcg agggcaacag
     4021 cggtaccacc acctactcgt tcacggtcag cctcagcgcc ccggcaccgg tgggcggcgt
     4081 cagcttcgac atcgccacct ccagcgactc ggccacgtcc ggcgtcgact tcgtggccaa
     4141 gagcctgacc gggcagacga ttccgcaggg cgcgagtacc tataccttcg acgtcctcgt
     4201 gaacggcgac accgcgctcg agatcaacga gaccttcctg gtggcgttga gcaacgcgac
     4261 cggcgcgaac atcgccgacg ccagcggcac cggcacgatc accaacgacg acatcgtcat
     4321 cacctcgatc ggcacgatcc agggcagcgg cgagctgtcc acgtacgacg gccagttggt
     4381 gatgaccgaa ggcgtggtga ccgccaagcg cggcaacggc ttcttcctgc agtcgttcgc
     4441 cgccagcggc gatgccgcca tcgacggcaa cgcggccact tcggacggca tcttcgtgtt
     4501 cacctcggcc gcgccgagca tggtcacggt cggcgacaac gcccgcgtca ccggcacggt
     4561 cgacgaattc ctgggcagcg ccgcaacccc aacgaccgaa atcgtctcgc ccagcgtgct
     4621 cgtgctcggc aacggcatcc cgctccccgc accggtggag atcaccgcgg cgctggccgg
     4681 cgccgacagc ctgccggatt cgctggagcg cctggaaggc atgctcgtgc gcgtggccca
     4741 gggcgtggtc accgcgcaga ccgacggcaa catcaacgag gccagcgcca cctcgagcga
     4801 ttccaatagc gcgttcgagt tcgtggtcac cggcgtctcg cgtccgctgc gcgagcccgg
     4861 catcagcatt ctcgacccgt tcccggtccc gccggcgaag caggccacca ttccctactt
     4921 cgatgccaac caggaacgca tcaaggcgtt cccgctggtc ggcacccgcc tggtcgccga
     4981 cgccggcgcg ccggtcagca acctggtcgg cgtgctgacc tacttcggcg attcctggga
     5041 actgctctac gacgtcgcca atccgccggt catcggcagc gccaccgcca ccgcggtcac
     5101 cacgccggtg gccgacgaca tcaccgtcgc cggcttcaac ctgttgcgct tcttcgacac
     5161 caccaacgac cccgccatcg gcgagccggt gctgacgccc tccgcctacg ccaaccgcct
     5221 ggccaagacc ggcgcggcga tctgcgactg ggtcaaggcc ccggacatcc tgggcgtggt
     5281 ggaagtcgag aacatcggca cgctcaccga cctggccaac tacatcaacg gcaattgcgc
     5341 cagcgcgcct gcgtacgtgc cgtacctgca ggaaggcaat gacgtaggcg gcatcgacgt
     5401 cggcttcctg gtcagcaacc gcgcggttcg cgccggcgtc tcccgcgtgg ccgtgggttc
     5461 ggtcacccag gtcggcaaga ccaccctgta cggcaccaac ccgctgggct gcacgccggg
     5521 cgcgccgggc tgcacctcct ccctgctcaa cgatcgcccg tcgctggtgc tgcgcgccac
     5581 cgtcaacttc gccgatggac gcatgtaccc gctgaccgtg atcgccaacc acctgcgctc
     5641 gctgaacggc aacgacgatc cggccgacaa ccgcgtgcgc ttcaagcgcg ccgagcaggc
     5701 gatggaactg ggccggctgg tcaacggcat gcaggtcgcc aacccgaacg agaagatcgt
     5761 gctggtcggc gacttcaacg ccttcccgtt caacgacggc tacgtggatt cgatgggcat
     5821 catcaccggc aacgccgcgc ccgagccgga agtcatcgaa taccgcgcaa gcccggtcgc
     5881 cacgcccctg gtcctgggcg atgcactgac cgccgatccg gcacagcgct actcctacgt
     5941 gttcgaaggc aatgcacaga ccctcgacca cgcggtggtc aatgccgcgc tggtgaacga
     6001 tccggccgtc gccggcctga gcgtcgagca cgcgcggatc aatgcagact tccgggtgtt
     6061 ccactacggc cagttcggcc tgccgtacac cgcgcagaac ccgccgctgc gcgtgtccga
     6121 ccatgacccg gtgcgcctga gcatccgcgt ggtgcgcccg gcgagcgcag acctggcact
     6181 ggcctggtcg acccgttcga tgacctcgaa gggcgcgttc gcaacgctca cgctgcgcaa
     6241 caccggtgcg tcgccgatca gcggcgcgca ggtcaggatc acgctggaca tcccggcgga
     6301 cgcgaccgcg gccgtgggcc cgccctccgg ctggacctgc acccggccgg ccgacgcctt
     6361 cgtcggcgac accgagttcc tgtgcaccgc cagcacgtcg gtaccggtgg gttccagcca
     6421 gggcttcagc gtcaccgtga agccgcgccg cagcttcgcc gccagcgacg tcctgcgctt
     6481 cgaggcgcgc gcgaccgtgc tcaccgaccc gatctccggg gacaacagcg cggtgctcgt
     6541 gctgccctga gccggatcgc caggtgaacc caaggcgcag cttcggctgc gccttttttt
     6601 tgcactgcgc aacagccggt taaccggcga accgtatcat tcgcaaccaa tgcatcactg
     6661 gatggcgcgg atgaccgtgg cggcgcgttt cgagatcgaa tacctgcagt acctcaagcc
     6721 cgacggcacc ctggcgggcg acctgccgcc tgccgttccg gatgcgaacg cattgctgcc
     6781 gctgttcaag cgcatgctgt tcgtgcgcac cttcgacagc aaggcgatcg ccctgcagcg
     6841 caccggcaag ctgggcacct atgccgcctg cctgggccac gaggcgaccc atgtcggcat
     6901 cggcgcgtcg atgcagccgg acgacgtgtt cgcgcccagc tatcgcgaat acggcgcgca
     6961 gttcgagcgc ggggtgaagc cgcgcgacgt cctgatgtac tggggcggcg acgaacgcgg
     7021 caacgatttc gagatcccgc gcaaggacta tccgtggtgc gtgccgatct ccacgcagtg
     7081 cctgatggcc tccggcgcgg cgctgtcctt caagctgcgc ggcgagaatc gcgtcgccgt
     7141 ggcgacctgc ggcgatggcg gctcctcgaa gaccgatttc tacgcggcgg tgaattccgc
     7201 cggcgcgttc accctgccgc tggtgctgtg cgtgatcaac aacggctggg cgatcagcgt
     7261 gccgcgcaag gcgcagacgg gcgccgacac gctggcgcag aaaggcctgg ccggcggcct
     7321 gcattgcctg caggtggatg gcaacgacct ggtcgcggtg ctggaagcca tgcgccgcgc
     7381 acacgaacgc gcgcgcagcg gccagggcgg cagcgtcatc gaattcatga cctatcgcct
     7441 gcacgaccac accaccgccg acgacgcccg ccgctaccgc ggcgaggacg aagtgaaggc
     7501 cgcctgggag aaggaaccgt tcctgcgcct gcgcaagtac ctgaccgacc agggcctgtg
     7561 ggacgagtcg caggagaagg cctggatcga ggaatgcggc aagttggtcg atgtcgagat
     7621 caacgcgtac ctggaaaccc cggtgcaacc ggtcgaagcg atgttcgact atctctacgg
     7681 cgacatgccg gcggacgtgc tggcgcaacg cgagcaggcc attgcgttgg agaagcgcgc
     7741 atgagcgagc aaccgattgc actgatcgag gcgatcaccc aggcgctggc ctacgagctg
     7801 cgcaatgacc cgagcgtggt cgtgctgggc gaggacgtgg gcgtcaacgg cggcgtgttc
     7861 cgcgcgacgg ccgggctgtc cgcgaccttc ggcaccgagc gcatcctcga cacgccgctg
     7921 gacgaaacca ccatcgccgg cctgaccgtc ggcatggcct ctcagggcat gaaaccggtc
     7981 gccgaagcgc agttcgacgg cttcatgtat ccgatggtcg acttcatcgt ctgccacgcg
     8041 gcgcgcatgc gctatcgcac ccgcggccgc ctgacctgcc cgatggtgct gcgcgtgccg
     8101 tggggcggcg gcatccgcgc gccggagcac cacagcgaag ccaacgaatc gatcttcacc
     8161 aacgtgcccg gcctgcgcgt ggtcatgccc tccagtccgc agcgcgccta cggcctgctg
     8221 ctggcggcga tccgcgaccc ggacccggtg atctacatgg agcccaagcg catctaccgg
     8281 cagtacaagg aactggtgcc ggacgatggc gaggcgctcc cgctggacgt ctgctacgtg
     8341 ctccgcgacg gcagcgacgt gaccctggtg acctggggcg cgcaggtgaa ggaaaccctg
     8401 gaagccgcgg agaaactcgc cgccgacggc atcagcgccg aagtcatcga cgtcgccacg
     8461 ctgaagccgc tggacttcgc caccatcgcc gagtcgatcg ccaagaccgg ccgctgcgtg
     8521 atcgtccacg aggccgccaa gaccgccggt ttcggcgccg agatcgccgc gcgctgcgcc
     8581 gaggaatgca tgttcgacct ggtggccccg gtcgagcgcg tcaccggcta cgacacccac
     8641 atcccgttgt tccggctgga aatgaagtac ctgccgagca ccgacaaggt catcgccgcc
     8701 gccaggcgcg cgatggcgca ttcgtaagga ccccgcatgt cgaagaaatt catgctcccc
     8761 gacctcggcg aaggcctgcc ggacgcgacc atcgtcgagt ggtacgtcaa ggtcggcgac
     8821 accatcaagc tcgacgacaa cctggtgtcg atggagaccg cgaaggcggt ggtcgacgtg
     8881 ccctcgccgg tcagcggcaa ggtgctgcaa ctggccggcg cggcgggcga catcgtggtc
     8941 accggcacga tgctggccga attcgaagtc gacgcatcca tgccgcagcg cgcggaaggc
     9001 caggacaccg gccaccacca cggcccgacc cacgaggaac cgaagcccgc ttcagcgccc
     9061 gagcccgcgg cgaaggcgga tgcaggcacc gtcgtcggcg cgatgcaggt cggtgacgcc
     9121 gtggtggcgc aagccgcgat cgccgccggt ggcgtcaagg cgatgccggc ggtgcgcgcg
     9181 accgcacgca agctcggcgt cgacatcggc cgcgtcgccg cgaccggccc cgatggcacg
     9241 gtgacgatgg atgacgtgaa gcgcgcggcg gcggatgggt ctgccaagcc gggggccgca
     9301 cctgctcgca gcggcgcaca ggcggccgtc gcgcaggtcg ctgcgcctgc ggtggcgtcg
     9361 cggagcacgt tgtcgcaatc cggcaagccg atgcgcacgc agcctccggg ggcgatcgcg
     9421 agtggccagc ccgagcaact gaagggcgtg cgccgcaaca tggcgcgggt catggccgat
     9481 gcgcacgcgc aggtggtacc gaccacgctg gtcgacgacg ccgacctgca cgcgtggagc
     9541 ggccgccagg acatcaccgc acgcctcgtg cgcgcgatcg tgaccgcctg caagaccgtg
     9601 ccggccctca atgcctggtt cgacggcgcc aacctcaccc gcaccctgca tccgcatgtc
     9661 gacctcggca tcgcggtgga taccgacgac ggcctgttcg tgccggcact gcgcaatgcc
     9721 gacatgctcg acggcaacgg cgtgcgcgcc gcgatcaagc gcctgcgcgc gcaggtcgag
     9781 gaacgttcga tcccgtcctc ggaactctcg ggttacacga tcagcctgtc caacttcggc
     9841 atgttcgccg gccgctatgc gacgcccgtc gtggtgccgc cctgcgtggc gatcatcggc
     9901 gccggcaagc tctcgcacga cgtggtcgca gtgatgggcg gcatcgaggt gcatcggcgc
     9961 atgccgatct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcgatgccg
    10081 ccaccgactt ctggtcgcag gcactgggct gcacgatcgc cgaccgcgat gccggcgatg
    10141 gcgacgccga gtaccagatg ctcggcgaca cgccgggcga cctgcacatc gaagtgcaga
    10201 aggtcgcgca cccctcgcgc gtccacctgg acatcgaaac cgacgacgtg gacgcggaag
    10261 ccgatcgcct cgaagcgctc ggcgcgaaga agatcgcatt cgtgaagcgc tggtgggtga
    10321 tggaagcgcc gaccggccag cgtttctgca tcgtccgcac gaaattcccg gaacgcggcg
    10381 tcgcctggaa cgactgggac tgatcagccc gtcgccagca tccggcgacg caggtaaaac
    10441 gcgccggtga gcaccgccag gaacacgccg taacccaccg aagtcgccag gatctgcggc
    10501 cacaggtggc cgtggctgcg atcggcgaaa ccgaaactcc agtacaggct ggccaggcag
    10561 ccggcgaacg cgagcagcag cgcgatggcg tcgaagcgca gtcgcgcggc gttgcggggc
    10621 tggcgcggat acaaccagta cagcgtcgcc aggatcagga accacggcgc gaacaggatc
    10681 agcgccagcg cggggatgcc ggcgacgttc acgccttgcc cgcctcgcct tccagttgcg
    10741 cagcgacgaa cgccgccagc gcggcattca cttcctcgtc gcccacgccc tcgcccagcg
    10801 cgaaatcgct ggcaccgtcg ccgatctcgc cctgcttgat cgcggcgatg cgctggccgg
    10861 catcgcgcgg cgaggcgaaa ccggtgctct gcagcagcac ccgttcgccc tgcaccagct
    10921 tgaaatagaa cttgccgtcg gcgtcgcggt actgcttgaa cgtcggcacg gtggcgcttg
    10981 cttctttctt cgtcgacttc gcatcgaccg tgctcacctg cgacaggtcg cgcagcccca
    11041 ccgcttcgcg caggcgtcgc agcgtgggcg tggcatgctc cgcgcgcagg cgcgacgcgc
    11101 catcgcgcag gatcgcctcg atctcgccgg gcctggccat caacgcttcg tatttttcgc
    11161 gcagcggcgt gacttcggca tcgatgcgtt cgaacagcgt ctgcttggcg tcgccccagc
    11221 cgatgccatc ggcgaaggcc tgcgcgaatg cggcggtgtc ttcggtggat gcgaatgcct
    11281 ggtaaagctg gaacaacgcg gaaccctcgg tgtccttggg ctcgccgggc gcgcgcgagt
    11341 cggtgaggat gccggcgacc agcttcttca gttcgccgcg cggggcgaac atcgggatcg
    11401 tgttgtcgta gctcttgctc atcttgcgac cgtccaggcc gggcaaggtg gccacgttgt
    11461 cgtccaccgc cgcttccggc aggacgaaga agtcctgccc gtagatgtgg ttgaagcgct
    11521 ggccgaaatc gcgcgccatc tcgatgtgct gcacctggtc gcggccgacc ggcaccttgt
    11581 gcgcattgaa gatcaggatg tccgcggcca tcagcaccgg gtacatgaac aggccagcgc
    11641 tcacgccggc atcgtcgtcc tcgccctcgg cgcggttgcg gtccaccgcc gccttgtagg
    11701 catgggcgcg gttgaggatc cccttgccgg ccacgcaggt gagcagccag gtcagctcgg
    11761 ggatttccgg gatgtcggac tggcggtaga accagacctt gtcgggatcc agcccgcatg
    11821 caagccaggc cgcggcgatt tccagcgtgg agcgctgggt gcgcgccggg tcctgcgcct
    11881 tgatcaggct gtggtagtcg gcgaggaaat agaagctttc cacgcccggc gcatgcgacg
    11941 cggcgatcgc cgggcggatc gcgccgacgt agttgccaag gtgcggggtg ccggaggtgg
    12001 tgatgccggt aaggacgcgg gtgtgactca tgcgaggcgc gggccgaggc tgtgatccgg
    12061 ggccgcacag tgtacccgcg gccccgccaa ccccgttccg gaagcccggg cgttggcatc
    12121 cgggcagcgc acttccgcgc taggagaccc gcaatgttcc gtcgcctgct gctcgccctg
    12181 tccctcgccg cctccgtggc ctgctcgacc ccggtttcag ccggcgacct cgtccagttg
    12241 gacgtggtcg accgcgacaa cggccaggtc ctgcccgaat accgccaccg cgggcaggac
    12301 tggatcgccg gcgtgcccgg ccatcgctat tcggtgcggc tgaccaacac caccggcgag
    12361 cgcgtactgg tcgtgctctc ggtcgatggc gtcaatgccg tcaccgggca gaccgcctcg
    12421 ccctcgcagg gcggctacgt gctggaaccc tgggaaaccg ccgagatcgc cggctggcgc
    12481 aagtcgttgg acgacatcgc ccagttcgtc ttcaccgacc tgccggacag ctacgccgcg
    12541 cgcaccgggc gccccgcgaa cgtgggcgtg gtcggggtgg ccgtgttccg cgagcgccag
    12601 gtgcagccgg cctacgcacc gccgcccgcc ccaccgatcg ccagtggcat ggcccgcaac
    12661 gaatcccgca aggcgtcagc gcccgccgcc agcgccgacc gcgcgatggc atccgaagcg
    12721 gaggccatgc cgcaacgtct gggcaccggc catggccagc gcgaatgggc gccggtcggg
    12781 cagaccgagt tcgtgcgtgc gagccgcagc ccgcagcagg tcagccagct gcgctatgac
    12841 gatgccgatg cgctggtcgc gctgggcatc ctgccgcact accgggcgcc gtatgcgggc
    12901 tccacgccgc gcgcgtttcc gaacggcttc gtgcaggatc cgccgtaccg gtactgaact
    12961 catcgccgcg aaaacgacga aggccggcgt gcgccggcct tccgttttcc gcgatgcgac
    13021 gaggctgact tcgcctactt ggtcgcggct tcctcgacct tctcgccgac cttctgcacg
    13081 tccttgccgg cacctgcgat cgtgttgcag cccgccagca cgagcatgca cagcagcatc
    13141 gccacgccca acctgcccaa cttcatcatg accgcctctg cttcagggtt gtgaacgcgg
    13201 cgagcttatc cagtcgggtc gatgccttgc aaccgccatc catcacgcaa gaggcggacc
    13261 gggttgcccc ggcccgccac gcatccttgc ggtcttccct ccttcgtcag tgcgaaggaa
    13321 gtgcgtgact cagcacttgc cgtccttgca gtcctgcgcc ttgtcttcga ccttctcgcc
    13381 caccttctgc acgtccttgc cagcgccggc catggtgttg caggcggtca gggtggacat
    13441 cgagaacatc gcgagcagca gcagtgcgaa caaacgcttc atgtgtttct cctcatgggt
    13501 tggccgattt cctggcaggc ccgccatcgc cggcccacgg acgtgatgaa accgcatccg
    13561 gtagtgaagc cggcgtgcac tcgaggcagc ttggccaaac cgcggatgaa ccgcggtctc
    13621 ccatgcgatc aatcgcgcat cagcgtcgat tttccgaaca ggctttccac caggtccacg
    13681 gcgagccgcg ccgtcgcgtt gcgcttgtcc agcgcgggat tgacctcgac caggtcgagc
    13741 gagcccatgc agccggtatc ggccaccatc tccatgatca attgcgcctc gcggtagttc
    13801 accccaccgg gcacgcgggt accggtgcct ggcgcgatcg agggatcgag catgtcgacg
    13861 tcgaagctga catgcaggtg ggtgtcggca tccacgcctt ccagcgcttc ctcgatcgcg
    13921 cggcgcatgc cgacttcatc gatgtagcgc atgtcgtaga tgtccaggcc atgctgctgg
    13981 accaggcgct tctcgccggg atccaccgaa cggatgccga tctggcggat ctggtcggga
    14041 gtgaccgcag gcgtgaaacc tgccatcccg gtcagcacat cgggaccaag gccgcacagg
    14101 caggccaccg gcatgccgtg gatgttgccg gacggggtga tctcgcgggt gttgaaatcg
    14161 gcatgcgcat ccagccacaa cacgcgcagc ttcttgtcgg tcgcgcggca gtgcgcagcg
    14221 accgcggcga tcgcgccgat gcccaggcaa tgatcgccac cgagcatgat cggcatccgc
    14281 ccggcctgga gttcgcgcgt gctcgcgtcg aacacggcgc gattccaggc gacgacttcg
    14341 tcgaggtggc gataaccatc gaccggcggg ctggtcgggt tgcgcgggcc ctgcacgtcg
    14401 ccgatgtcgc ggacgtcgat gccgcgcgcg gcgatcgcct cgaccaggcc ggccacgcgc
    14461 aaggcctcgg ggcccatcga agcaccgcgg cgggaggcgc cgatgtcggt gggcacgccg
    14521 aacacggaaa cggggggagt ggtgcgggtc atcggaagtc cctcatctgc gctgcggtaa
    14581 ggacacgcaa cgcctgcgtt gccggcggtg gctggtgccg cttatccgaa tcgaacggat
    14641 gacctaccgc ttacaaggcg gttgctctac ctgctgagct aaagcggcgc tgggcccaat
    14701 tctagcccgg cgccgccggc gccccaacca cggctcagcg caaggtggcg caatcgatcg
    14761 cgcgcgacgc ggcgatcgcg gcgagcgacg ccgggtcggt ggcggtgggc aggcgggcgt
    14821 ccagccaggc ctgcgagggc gtgtcgccga gcggcatggc ctgcgcctgg aagcccttgc
    14881 cgcgcagttc tgcctcgcgc cggcgcgccg cgtcctcgct gccgaaacga cccagggcga
    14941 tgctgttggc gtccgcgcct tcgttcatca cgtacaggtc gctgatgccg gcggccttga
    15001 tccgttcggc catcgccacc gcgtcctcgc gcgaggccag cggcggcatc gccaccttcc
    15061 agccgcgcgc cgagcgggcc gggctgtcgc gggtcaccgc gctcacgccc gccgccatga
    15121 gcgcattgcg tgcggcatcg cgcgccgtgc cgtccgcgaa cggaccgaag cgcaggcaga
    15181 cgggcgcgct ggccggggcc gccacgaccg atgtcgcggc cggcaccatg gacgcgactg
    15241 gtgcgggtcc cgcgggtgct ggttgcgcac gcgttggcgc ggtcgcgacc gccggctcgt
    15301 tgaccaggcg caggccgggg gcaccagcgg cattcgcctc cgccccgcgc gcgtctgcca
    15361 cggagggacg cagcccccac caggcggcgg cgcccaggtt aagcatgaag aggatcacga
    15421 gggtcgcgcg cagcagcatc cgggcagttt atgcgatgcg cagcgcatgc caacgcgcca
    15481 gcccgcgcag caccgcgtcg ggttgccagc ggtggccggg caagcggtca cgcaagagat
    15541 ccgcaccgcc gccgtgcagg cacacggccg gagactcgcc caacaacgcg gtcgcggccc
    15601 ccaggctgcg ttcgaccagc gccagcgcag caccttcgca acccgaggcc aatgcgtcgc
    15661 cggtatcgtc ggcgaactcg gcgtagatgc cgccctcgac cggcaactgc gcagcgcgtg
    15721 cgtgcagggc ctcgcgcatg gtccgcggcg agggcgcgat gcgtccaccg cgatgcaggc
    15781 cgccgccatc gagcaggtcg agcgtcagcg ccgtgcccac gccgaccacc agcgacgcgc
    15841 cttcgccgca ctgcccgagc atcgaaagga aacgatccac gccgagatgc gccgggttgt
    15901 cataggcaat gcgcagcggc ccgaggacgc gctccgtgcg cacgcgatgc aggcgggtga
    15961 aacgcgcgca caatgcatcg aacaacgcgg cacggcggac atccgaggcg acgctggcga
    16021 tgcaggccac gctgccggag ggcagcgcat cgagccaggc ggtgccgtcg tcgtgggcga
    16081 tggcaagggg ctcgtcgatg ctgccatccg catgcagctg cgcgcacttg aggcgcgtgt
    16141 tgccgaggtc gaacagccat gtcctcacag gcgcacgctc acgtcgccgg catgcacgcg
    16201 acgttcgctg ccggatgcgt cgcgcacgcg cagcgcaccg tcgtcggcga tgccgagtgc
    16261 ggtcgcatgc catgcgctgg tggtgtcgtg gatcgcgacc tcgcgtccgg ccagcgcatc
    16321 gaagccggcg taacgcgaca ggaatggcga caggccctgc gcgtcgaatg cgtccagcgc
    16381 cggcagcaag tgcgccagca gcgcggtcac caccacattg cgcgacggcg gtcgcgccga
    16441 gagtcccgac agttcggccc agggctggtc gatgccggcg ccgtcgcgtg ccggcatccg
    16501 catgttcagt ccgatgccga tcaccgcgcg cgccgcgccc gccgtttcgc cgccgccctc
    16561 caccagcagc ccaccgagct tgcgcaggcc cgcgccatcg accagcacca ggtcgttagg
    16621 ccatttcagg cgtacgccat cgaagccgag accatgcagg gcgtcgcagg ccgccacgcc
    16681 agccaccagg ctcagcccgc ccagccgcgc caagccgccc tcgaaacgcc gcgacaacga
    16741 caggtagaca tgcgccgcca acggcgaggc ccatgcgcgc ccgcgccgcc cgcgaccacc
    16801 agtctggcgt tccgccagta ataccgcgca accgtgcgcc ggtgtgtcac gacgcagcaa
    16861 ttcgctgttg gtggaatcca gcgaccacgc aacttcgaga tcgaccaacc caccctgcac
    16921 cgaggccggc aaccccgcgc ggatcgcatc ggcatccagt aggtccagcg gttgtcgtag
    16981 cgcataaccg cgcccgggcg atgcgccgat atcgatgccg gcctcgcgca gggcatcgat
    17041 ccgcttccag accgcggcgc gggtcagccc ggcctcacgc gccagcgtgt cgccactcac
    17101 cggcccggcg gccagccgtt gcaacaagcc gcgctcctgc tgcgcatcgc cggcccgcat
    17161 cgttcagtac cggctgcggc cgtacaggcg atagagcagg cgcgcgcgct cgaagcgcga
    17221 ttccttgcca tgccacagcc gcaccaggtt ggcgcggtgc gtccatgcca ggaacaggcc
    17281 catcccgatg cagaaccaca ttcgctccgg gccggcatcg aatccccacg ccagcaacgg
    17341 caggcacagc gcggcgacca cggtcgacag gccgacgtag ccggtcgaca ccagcaccag
    17401 gatccaggcc aacagcagca ggggcacgat ccatggccac agcaccagca ggccgccgac
    17461 accggtggcc gcgcccttgc cgccgcggaa gccgtgccac aacggccaga catgcccgac
    17521 caccgccagc gcggcggcga ggtaaccatg tgccgtcacg ctcaacccgg tgccgacggg
    17581 cgcataacgc agcaccagcc aggtggccaa cgcccccttg gcgacgtcga tcacggccac
    17641 gcccagcgcg aatttccagc ccagcgtgcg cagcgcattg gtgccaccgg cattgccgct
    17701 gccgtgttcg cggatgtcga tgttgcggaa gcgcccgagc accaggctgc cggagaccga
    17761 tccgagcagg taggccagca acagcaggag cgcggtgagc aggatcggcg gcatggcgac
    17821 gatgataatc gcccgggcag cgtcagtcgg gtgacggctg cagccccacc acgagcgtgc
    17881 cctgcccggc gtgtgcgccg accgcgggac ccacttcgac caggtgcgct tccaccaccg
    17941 gcaggcgttc gcgcaatgcc gcgagcaggc gctcgccatc ggccagtgca tcgctgtggc
    18001 cgaccaccac gcgccagccc ttcgacgccg gcgtgcgcct ggccacgtaa cgcgcaaacg
    18061 cctccggggc gcgctgcttc gcgaacaagc cgccggccac cttcagcgtg cccgcatcgg
    18121 acaccgccgc aaccggcgtc agcccgctcc agcgcaccac cggtccggcc cagcgcggga
    18181 tgcggccgcc gcgcaccgca tgcgagatgt cgcgggccat cgcccaggtc ttcgtcagcg
    18241 gacggagcct ctgaagttcg cgttcgatcg cgtcgccatc ggctcccgcc gcggccatct
    18301 cgccggcccg ccatgcgagc agggactcgc cggcgctgac atggccgctg tcgatgcaac
    18361 gcaccggctt gtcgcgcccg cgcgcggcgg tttcgcccga ctgcagggtg ccggacaagg
    18421 ttcgcgagac gcccacgtag accgcgcccg gctggtaggc caggacgtgg tcgaacaccc
    18481 tgcggaaatc gcccggcggc ggctggctgc tctgcggcaa ggccttgctg gcggccatgc
    18541 gccggtagaa ctcacccgcg gtcaacccga gccggtccag gtaatcgcgc ccatccaggc
    18601 tgatccgcgc agggaccacg tggatgccgt atcgctcggc gatcacggcg ggcaagtcag
    18661 aggcgctgtc ggtgatgacc gccagcagcg agggcgattc cgtgctgcgc tgctgcagca
    18721 ccatgtcatc ggccttcatc gcatccacgt ggccaaaccc cgcgcaggca tcgaacaggc
    18781 gctggggcgt gcccacatgg ccgtgcacgc gcacccgggt ggcgctgccc gcgaccacca
    18841 gcgagtcgac accggcaccc gccagcgccg cctgcagggc gacgcggtcg atgccgtcgc
    18901 cggtcaccag gcactccgtg cagtagcgat gcgcgggatc cacgtcctcg tgcacgtggg
    18961 ccggcatcgg gcccgcgtcg ttggccgccg cggtcgcgtg gccgtgcagg cgcaccgcgc
    19021 gcggcccacc gtcgacgtat tcggcgatcc cctccagcca gtcgacgaat ccgcgcgcgc
    19081 cggcatcgac cacgcccgct ttctgcaaca gggccatctg cttgggggta tcggccagcg
    19141 ccgtccgcgc gcgctgcaag gcccgcgcga agccgccctt cggatcgccc tgcggctgct
    19201 tcgccgcctc atccagcgca tcggcgaacg cggagatcac actgaggatg gtgccctcga
    19261 ccggctgcgc caacgccgcg cgcgcactat ccgcgccacg ccgcaccgcc agggccaggg
    19321 tggcggcatc cagcacgggg gcgttgcgcg cctgttcggc cagcccaacc aggaactgcg
    19381 ccatgatcgc gccggaattg ccgcgcgcac cgtcgatcgc gtcgttgccg atcccgacca
    19441 gcaattcgcc gatgtggcgg ctgcgccggc tcagcgcgcc ctgcaggacg ctgcccaaag
    19501 tcgatgcgag gttgctgccg gtgtcgccgt cggcgaccgg gaagacgttg atgcgattga
    19561 ggtcgtcgcg agccgcgatc acccggcgcg cgccggcgat cagcgcgcgg cgcagggcgg
    19621 gtgcggtgac ggcaggcggg tcggcggtgg ccactggcat gcccgcagtc tgcggtcgat
    19681 ggcggcgcaa tgcctgctgc tttgcagcat cgcaaccttt cgtgcccggc gcgcatccac
    19741 gctatagtgg gaggtgggga catgccttcc gcactccctt ctttttccgg tgaccaccat
    19801 gcgcctgcgc ccgatgctgc tttgccttgc cctgctggct gccagcggcc acgccatggc
    19861 gactgagtcc aggcaatcgg cgagcgacga agcctgcccg gacacgaccg ccgccagtgc
    19921 cagcgaacgc accgacgatg gccagccggc cgaggaaccg accgcgccga cccggcagac
    19981 ccacaaggca aagcccgcgg tatcggcttc cccgcgcgcg acgggcggca cccgcaccag
    20041 cccgccgcgc ttccacagct tccttccggg catgttccgc tgaggacacc cgtgttgcct
    20101 gatccgttcg cgtgatctgg ccgttctcgc gccgcaaggc gacgctcccg ctcccccttc
    20161 tcgatgacac gctctggcgg caggcagccg ctggcctgcc atggctgcgc cgcatcgatg
    20221 ccgaccgcgg gcaacgcctg cgccagctcg tcaccgaatt ccttcgcgac aaagcgatca
    20281 ccccactgca ggggctgcgc ctgaacgagg tccagcgcct gcggctggcc atgctgtgct
    20341 gcctgccgct gctcgaattc ggccgcgaag gcctgcacgg gtggtcgcag ttgctggtgt
    20401 atccgcaagc attccgcgtg cagcgcaatc acatggatgc gcagggcgtg ctgcacgaat
    20461 gggacgacga actcatcgga gaagcgtggg aacgcggccc ggtgatcctg tcctgggccg
    20521 acgtgcgcgc cgacatcgcc acgcccgatg acggcttctg cgtggtggct cacgagatcg
    20581 cccacaagct cgacgccttg gacggcgcga tggacggcac cccgccgttg ccgcgcgact
    20641 ggcaacggca gtgggcatcg gatttccagc gtgcgtacga agggttgtgc aatgccgtcg
    20701 atgccggccg cgagaccacg atcgacccgt atgccgcgga atcgccggaa gagttcttcg
    20761 cggtatgcac cgaacatcac ttcagcgatt ccgcgaccct gcgcaaagcg atgccggacg
    20821 tggcggcgca tttgcagcgg ttctacggcc cgccattgct ggcttgacgg ctcccctctc
    20881 ccgcaagggg cgaggtgaaa atcacaaccc cggcggcaac accacctcga acttcgcacc
    20941 gccgagttcc tgcgaacggc ccacgtgcaa ctcgccgcga taggccttga cgatgtcctg
    21001 cacgatggcc agtccgatgc cgtggccctg cacgcgctca tcgccgcgca cgccgcgctg
    21061 gagcacgtgg gcgatgcgat cgtcggcgat gccggggccg tcatcctcga cggtgatcga
    21121 cagccccggg cggcgacccg gcaccggcgt gccctcggcc acggtcagca acaccttcga
    21181 tcgcgcccac ttgaacgcgt tctccaacag gttgccgagc agttcctgca ggtcaccggt
    21241 ttcgccgtgg aagcgtgcct cggcggcgat ctcgaactcg cagagcacgc gcttggcggc
    21301 gtagaccttt tccaggccgg tgacgatctg ttcggcctgc ggctcgatct cgatcggcgc
    21361 tgcgaacagc tggtggccgg agcgtgcggc acgcgccaat tggtaaccca ccaggtcgtt
    21421 catccgccgc agctgggttt cgacttcgtc gcggagttcg gattcgccgg caccgctgtc
    21481 caggcgcgtg cgcagcaccg ccagcggcgt cttcaggcta tgcgccaggt cggccagggt
    21541 gttgcgctgg cgatccaggt tctcgcgctc gccttcgatg aaggcgttga tgctgtccgt
    21601 caacggttcc agctcgcgag gatgcgcctc gctcatgccc gcggcctggc catgctgcac
    21661 acgcttgagt tcggcgatga cgtcgcgcag cgggcgcagg ctccagcgca gcacgagcat
    21721 ctgcaacagc aggaggacga cgccggcacc gcccaggtaa cgccacagcg cggagcggaa
    21781 ggccgccacc tgccgcccga gcgccgtcgt gtcttcgagg atgtagacgg tgtaggggaa
    21841 ttcgcggcgc gaatcctcgc tgccggtcca gagcaggcca tagccatagc ggtacacctg
    21901 gccggcgccg ccatcgatgc gggtcagcgg caacggtcct tcgaaaaccc gctggccggc
    21961 gtcgagcatt gccccttccg gcaaccgtgg gccctgcgcg gaatcggact gccattgtcc
    22021 cgacggcagc aggatttgcg catacagccc gctacctggc cgccggaagc gcgggtcggg
    22081 tgggtcgtac ggcgggatga cgctgccatc gcgtgcgaag tcgctgttgg cgtacgccag
    22141 cgcgtagctg tgcaggcgct cgcgcatgcc gctctgggcg gtgctctgga acgcacgatc
    22201 cagggcaaag ccggccaacg cgaggaaggc gatcagcccc aagctggccg cccacaactg
    22261 ccgcgattgc agggagcgcg gctgggcgat gcgcagccga cgcaatcgcg ttgcccggct
    22321 ggtgcccgcg ccttcctcgg ccggctcggg cgcgatcacc ccgtcaaatc ccgcgggaaa
    22381 ctcagtcgcc gctgcgcgcg atcgcgaagc ggtaaccgcg gccacgcacc gtttcgatcg
    22441 gcttgagggc gccgtccgga tccagcttct tgcgcaggcg gccgatgaag acctccagca
    22501 cgttggaatc gcggtcgaa
//
LOCUS       DAOOCX010000032        22297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2004_length_22297_cov_5.399784, whole genome shotgun sequence.
ACCESSION   DAOOCX010000032 DAOOCX010000000
VERSION     DAOOCX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 22297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22297
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2004_length_22297_cov_5.399784"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PK399_04800"
     CDS             complement(<1..1216)
                     /locus_tag="PK399_04800"
                     /inference="COORDINATES: protein motif:HMM:NF005566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPW12473.1"
                     /translation="MSSQRRIIIVGGSLGGLFAANLLHRAGWDVHVYERVAEELEGRG
                     AGIVTHPELIDTLHAAGVPEATDIGVTVSERVTLARDGSVAGRHALAQVLTAWGRMYQ
                     VLKAAFPADRYHNGHQLEALQQGADAVVARFTNGAAAEAELLVAADGIRSTVRQRLLP
                     QVRPVYAGYVAWRGLVDEAALSERTRRELCPFFAFGLPPREQMIAYPVAGPGHAVEPG
                     RRRFNFVWYRPADEASTLREMVTDATGKTWPEGIPPPLIRPEVLHGARQAAREVLAPQ
                     FAEVVCKTEQLFFQPIFDLESPSLAFGRVALLGDAAFVARPHCGMGVTKAAGDAMSLV
                     RALSADAEVPGALRAYEAERLRFGESIVTHARALGAYMQAQLLTADERAMAERYRSTE
                     AVMRETAVPPAMH"
     gene            complement(1253..1723)
                     /locus_tag="PK399_04805"
     CDS             complement(1253..1723)
                     /locus_tag="PK399_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW12474.1"
                     /translation="MPINKLAHFSVRTTSIEASRRFYTEVLGFREGYRPPFEFPGLWL
                     YSGGDEADYGVVHLIGSDRDDPDGLVRYLGDKDAASLHGSAAVDHLAFLATGLEDMRG
                     RLQRAGLPTRERTVPDLGLHQVFVEDPSGITIELNFPAAEAENAKPAGHPAAAC"
     gene            complement(1823..2527)
                     /locus_tag="PK399_04810"
     CDS             complement(1823..2527)
                     /locus_tag="PK399_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW12475.1"
                     /translation="MASRSVSNAHRDRDAAAVSSVAGGAPRRRLAPEDREHQILQAAI
                     GFFARHGFEASTRDLARELNITQPLLYRYFPTKEALVDRVYEEVFVRRWNHEWEEWLA
                     DRSVPLRTRLVRYLRDYAGFVLRNEWVRIFIYAGLSRGGINQRYLARLRERHFLLIAS
                     ELRHEFGIPEPAGAAEEDDEVELVWAMHSSVFYIGVRKWIYELPTPKNLDRLIDQRVD
                     AFLLGVPAVLQAARRR"
     gene            2650..3867
                     /locus_tag="PK399_04815"
     CDS             2650..3867
                     /locus_tag="PK399_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW12476.1"
                     /translation="MKSAKLASTLLPRAVSAVALASAAVLSHAQTIKIGLPVPLTGPY
                     GAEAKDQVRNAELAVKQFNDAGGLHGRKAELLVRDDKLNPGEAATRTLELIEKDGAQF
                     IVGGLSAATQLSINNVTRQRKVIYVSISQSDVINEPPDASPYTFHEALNPYMTTQAVG
                     RHVFKKGMKIAFLTADYAYGHEMTRGFTRVARDVGAEVVGEVKHPLGAQDYSTFLPRL
                     KALNPDVLVFNNFGADNRIAIKQAGDFGMKKAMKFVTPILTYTARVAGGAGVYEGVVG
                     GTQYYWELQDNTPSAKAFNAAFQAANGGRYPSDYGAMAYSAVKSLLAAVRTAGGTETD
                     KVAQALRSAPYDSYKGRQFYRACDQQSVQSVLLVESNAKDAAHPDDVFKLLHVEPASE
                     SLLRSCQELGFKG"
     gene            3878..4759
                     /locus_tag="PK399_04820"
     CDS             3878..4759
                     /locus_tag="PK399_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW12477.1"
                     /translation="MNLTVDLLALQLFTGVALGAIYVLLAVGLSLIFGMLTVVNFAHG
                     AFFMLGAYAGVWLADRTGSFWWALLLAPPLVAALGMLCERWLIRPLYGRGIDYPLLLT
                     FGLAYMMVEAVRMVFGKAALPFDTPEALQGATSLGFTDFPTYRLFVIAVVVALLLALW
                     LLIEKTPFGLIVRAGARDPVIVRVLGVDVSRVWLVVFGVGTGIAALAGVLSAPLQGVS
                     PEMGTSVLTVAFVVTVVGGMGSLMGAVVAGLLIGIVESMAVLFFPEAAKVSMFVIMAA
                     VLLLRPQGLFGRAGLLA"
     gene            4774..5766
                     /locus_tag="PK399_04825"
     CDS             4774..5766
                     /locus_tag="PK399_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPW12478.1"
                     /translation="MSSALKRTVPASAHKMDRWLQQVGLHRVAATVLIFACLPWVMPY
                     PSLAINILIYGLYAVGFNLLFGYTGLLSFGHAAFLGVGAYTAGILIAQHGASWWWAIP
                     AAVAMAALVALAMGLLAIRTRGIYFAMVTLALSQCVYFIVYQLPASGGENGLRGVNLA
                     EVSVAGLKLNLLDPMQKYYFVVVFVAAAMFVLSRILASPFGGVIEAVRENESRARACG
                     FDIERTKLLAFVLSAAFCGLAGALNAIHLSSVPIETLHYSTSGMVVMLCLLGGMGTYF
                     GPFVGAALFLGVQDLASAYTENWQLYVGALFVLFVLFFPKGVWGSFLERIRHGR"
     gene            5756..6508
                     /locus_tag="PK399_04830"
     CDS             5756..6508
                     /locus_tag="PK399_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW12479.1"
                     /translation="MAVDTIIETRGVSKRFGAFTALKDVSVKVPRGRLTAIIGPNGAG
                     KSTYFNVLSGAFAPSEGRVLFDGRDVTGMKPHDYAHTGIAKSFQITNLFPSFSVLENV
                     RIALQAHVSRYGLWRSRAALQGLQDQAMGLLLTVGLADCAKRLARELAHGQQRALEIA
                     VALAARPKLLLMDEPTAGMSPEETKVMMGLIRRLVATHTVVLVEHKMKMVMGISDHIV
                     VLHHGELLAEGRPEDIRRDEMVKMVYLGQGQH"
     gene            6532..7236
                     /locus_tag="PK399_04835"
     CDS             6532..7236
                     /locus_tag="PK399_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW12480.1"
                     /translation="MLQVKGLHAWYGTSHVLQGIDLEVAAGELVCLVGRNGAGKTTTV
                     KSVAGLLPKVQGSVVFEGREILGLPAHDRFGLGLAYVPEERRIVPGLTVRENIRLGLL
                     ASPLRRREADVIDELAQTFPRLKERLDQQAITMSGGEQQMLAIARAVASKPKFVLLDE
                     PSEGIMPLLVEEMFQYFTALKRSGVTILLIEQNVELALRIADRAYILDQGQVVYTGAA
                     DEMLADEAIQERYCAV"
     gene            complement(7252..8175)
                     /locus_tag="PK399_04840"
     CDS             complement(7252..8175)
                     /locus_tag="PK399_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPW12481.1"
                     /translation="MQGLKPGRSSDRLELFETFVRIVDAGSLSAAAAQLGTSQPTISR
                     RLKGLEEHLGLRLLQRSTHVMKLTEDGERCLALARGLLETWTALDADLRGAAAHPEGT
                     LRVVAPHAFGQDQLIGPVTDYLRQYPGVTVEWLLHDRRPDFIAEGVDCAIQVGVVDDP
                     NVVALKLGEVRRIAVAAPALLGRQVPSHPQDLASMPWLALRTFYRDEVALHSLVDETV
                     VAFPIRPRFSTDSLYALRSAAVRGLGACISSSWLVRSEIEAGHLVHLVPGWEAAPLPV
                     HLVYPHSRIYAPRLSRFIDAMRRLAPAAVSP"
     gene            8346..9512
                     /locus_tag="PK399_04845"
     CDS             8346..9512
                     /locus_tag="PK399_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW12482.1"
                     /translation="MLAVSAGFSVAALYYAQPMLGVVGATFDAGSQALGLVPTLTQLG
                     YAAGILLLAPLGDRFDRKRIILTKGFVLTLALVLSAIAPSLAWLLGASLVVGLSATVA
                     QDIVPAAAILAPETSRGKAVGTVMTGLLLGILLSRVVSGFVSEQLGWRSVFIVAGAGM
                     AVIVALLWRCLPNFKPTTTVRYGALMESMLALWRRHASLRRAAWSQGLLSVGFSAFWS
                     TLAILLHGEPFHLGSAAAGAFGLAGVAGALAAPLAGRMSDRRGPEVVTRWGASVAAVS
                     FAAMALTPLLSPTAQLWLIAASAIGFDFGVQAALVAHQTIVYGIEPAARSRLNAVLFA
                     GMFAGMAAGAGLGSALFAHWGWIGVVGLACASAMGASVIRMLPMHHLQLASQAR"
     gene            complement(9559..10449)
                     /locus_tag="PK399_04850"
     CDS             complement(9559..10449)
                     /locus_tag="PK399_04850"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPW12483.1"
                     /translation="MKGLQQLLAFAETAKHGSFAAAARELGTSSSTLAKAVSRLETSL
                     AVKLFHRTTRQVSLTTDGERLFHRCQRVLAEVEDLHVEAAGARMEPTGTLRIDMPIVF
                     GREVLLPLLGTLLDKHPGLQLDVRLQDGYADPVKEGLDLVVRVGDLRDSSLVARRFAS
                     QALVLCAAPAYLERHGTPRTLEQLSGHHGVMFRLPSTGKHRPWQLRSRGQPVTLHPPS
                     RVSVNDGDGIVQAVRAGLGIAQVPHYFVERLVATGEVVELLPSCRPAPMPISAVMPSG
                     RLVPPRVRAALALFDTLRER"
     gene            10598..10972
                     /locus_tag="PK399_04855"
     CDS             10598..10972
                     /locus_tag="PK399_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW12484.1"
                     /translation="MYVHDQTSPAPTALPGIAHATWAGIAENVGLSLWRQTVAPGAGT
                     PPHRHDCDEVVLCTAGYGELHIAGQVQRFGPDQTLTLPRGVEHQFFNVGALPLEVLGI
                     FAASPVGTFLPDGQAIDLPWRS"
     gene            11010..11414
                     /locus_tag="PK399_04860"
     CDS             11010..11414
                     /locus_tag="PK399_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12485.1"
                     /translation="MSNWLLRIATLYLAAGVTLGIAMAASHNHVEAPLHAHLNLLGFV
                     TLTLAALWYRAQPEASDTTLAKMHFVLHNLGLPVMSGGLFFLLRGEPAAEPVVAIGST
                     VVAAGIACFVVNLWRCTGHRKSTLDALATALR"
     gene            complement(11411..11914)
                     /locus_tag="PK399_04865"
     CDS             complement(11411..11914)
                     /locus_tag="PK399_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12486.1"
                     /translation="MTHAHEMTTEDRSLNLIARPRSKRWIALVAAIATAWSLSGCGGG
                     AYVDSAGNVTFDIGITVAGQFVNDTPVVSGGSLDLAVRAGQSVILDAGEPVVWTMYVG
                     GTAVGSGAQVYYAGADITGTTVSRTSVAVDTYAAYPLPASIPITLVATSTYDSAQVAT
                     VHLLITN"
     gene            complement(12502..14130)
                     /locus_tag="PK399_04870"
     CDS             complement(12502..14130)
                     /locus_tag="PK399_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HPW12487.1"
                     /translation="MNGLMMQQPLLISSLLKHAQTQHGDQEIVSRRVEGDLHRTTYRE
                     LAARARRMANALAALRVPVGSRVATLAWNGYRHMELYYAVSGSGSVLHTLNPRLHPDQ
                     VVWIADHAEDQVLFFDLTFLPLIEAVAPRVKTIKTFVAMTDRAHMPATSTVPNLLCYE
                     ELIADAPDAFAWPQLDENTASSLCYTSGTTGNPKGALYSHRSTVLHTYAAALPDALNC
                     SARDVILPVVPMFHVNAWGLPYVACMVGAKLVFPGPFLDGRSLHELFEGERVTMSAGV
                     PTVWQGLLAHVEANQLGFGTMRRTVIGGAACPPAMMRAFQERYDVQVLHAWGMTEMSP
                     IGTVCTLKPKHADLGADERMAVQSKQGRPVYGVELKIVGEDGRKLAHDGKASGELMVR
                     GPWIIQSYFKGEGGDPLIGGWFPTGDVSTIDADGYMQITDRSKDVIKSGGEWIGSIDL
                     ENIAMAHPGVAMAACIGAKHPKWDERPLLVVVRKSGSTVTRDELLAFYDGKIAKWWTP
                     DDVVFVDAIPLGATGKMQKNRLRELLADYPLPTA"
     gene            complement(14163..15494)
                     /locus_tag="PK399_04875"
     CDS             complement(14163..15494)
                     /locus_tag="PK399_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW12488.1"
                     /translation="MDLHSIEKTPAQDGYLFSRGQAWFAFGMTLALMVFDYVDRQVIV
                     SLFPYLKAEWNLSDKQLGALVSVVSVTVALGALPVALFADRVSRVKSIVAMATVWSLA
                     TISCMWTRGYGQLLAARAVVGLGEAGYGSVGAALIASHFPARMRGALMAGFFASASVG
                     SVLGVMLGGMIAERWGWQAAFGVVGVPGLLVALAYLKVRDYRTVSLEPALDAKRRSIG
                     EAVRHIVRVLARTRTMLWVCLGGAAQLIVVSALWSWLPSFLNRVHGVAPAQAGVKAAL
                     VVLAGALGSVVWGTLVDRVGVRHPRRKLVTMAVLCVLSMVLLVTAFGTSLAPPAQFAL
                     IAIGGFVATCTVGPVSAIVIDVIHPGVRATGASVLALFQNLFGLAAGPFVAGLLSDAW
                     GLVPALTVIPLCALLAAGACLMAARSYESDKARAAEPLAPEPATAMRSALA"
     gene            complement(15506..16477)
                     /locus_tag="PK399_04880"
     CDS             complement(15506..16477)
                     /locus_tag="PK399_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HPW12489.1"
                     /translation="MTRAIRLHQTGGPEVLQLETVDVGDPGPGQARVRHSYIAVNFID
                     IYFRTGRYPLPLPNGLGSDAVGVVEAVGAGVTDIQVGDRVGYLLGPQGAYAEVRVMPA
                     DVLIPLPEGISDRTASTLMMKGLTAQYLFRQVYPLQGGETILYHAAAGGVGLVACQWA
                     RALGVTMIGTVSTDEKAEIARAHGCTHTIVTSREDIAKRVRELTDGKGVPVVYDSVGK
                     DTLMASLDSLQPRGTLVSNGTTSGPVVIDTTLLAVKGSLWVTRPAMIHYATPRSHMLA
                     MADELFGHVLAGRIRGEPNQQFALADAAEAHRALESRRTTGATVLVP"
     gene            complement(16548..17144)
                     /locus_tag="PK399_04885"
     CDS             complement(16548..17144)
                     /locus_tag="PK399_04885"
                     /inference="COORDINATES: protein motif:HMM:NF018525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPW12490.1"
                     /translation="MSNEPCILIVPGLRDAVAEHWQTLLEMRLRAARRQVAVVQPMGR
                     ADLDCAARVAAIEQQAQAVQGPLVVVAHSAGCIMVAHWAQQTRCVVAGALLAAPPDFD
                     APLPAGYPTLAALQAGGWLPVPRCRLPFRSIVAASRNDELGRFERIAALAGAWGSRLD
                     DLGRVGHLNPASGFGTWPQANDYIDELCAGDAAHRRAA"
     gene            complement(17149..18207)
                     /locus_tag="PK399_04890"
     CDS             complement(17149..18207)
                     /locus_tag="PK399_04890"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPW12491.1"
                     /translation="MNFLDGSLFPENQDKLVITAAPYGPEWMPSDFPEDIPVTMEEQV
                     QKAVDCYNAGATVLHLHVRELDGRGSKRLSKFNELIAGVRAAVPDMIIQVGGSISFAP
                     ESDGAAAKWLSDDTRHMLAELDPKPDQVTVTVNTTQMNVLEHMDEADIAGTTLATPEG
                     RRAYSEMIVPSGPAWVEEHIRRLSAAGIQSAFQFYNINSFETVERMIRRGVYKGPLVM
                     NWVAISGGMDAPNVYNLANFLRAIPDGAMLTVESSMRNVLPINMMGLAMGLHVRCGIE
                     DNLWTQDRKAKMGTVRQIEQLVRLSREFGREVATGKEAREICQIGVFYDSVEETLAHN
                     GFAPNARPGLQGYLRKAA"
     gene            complement(18244..19152)
                     /locus_tag="PK399_04895"
     CDS             complement(18244..19152)
                     /locus_tag="PK399_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HPW12492.1"
                     /translation="MTTPRPESPAIVRAASLPDSIRVQPLTCAIGAELSGVHLGVASR
                     DAGLVAEIRALLLKHRVLFFRDQDITRAEHVAFARHFGDLEDHPVAGSDPEHPGLVRI
                     YKSPEQPNDRYENAWHSDATWRERPPFGCVLRCVECPPVGGDTMWANMVLAHDRLPES
                     VKAQIADLRARHSIEATFGAAMPIERRLALKAQFPDAEHPVVRTHPETGEKVLFVNAF
                     TTHFTNFHTPERVRVGQDYTQGSSQLLQYLISQAQIPEYQVRWRWQPNSVAIWDNRST
                     QHYAVMDYPLCHRKMERAGIVGDKPF"
     gene            complement(19264..22101)
                     /locus_tag="PK399_04900"
     CDS             complement(19264..22101)
                     /locus_tag="PK399_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPW12493.1"
                     /translation="MRWDFGGSDLRQNPAASAAGRSDANPRTIICRAVGHRRTALRPT
                     PPSLARYVAAAAPPALVGPTDDLLLRVTAPRVPRHHVARPRLKADGEPFDQPIVLVQA
                     PAGFGKTSLLAQWRREHLARGAVVAWLTAQTHDDAHRLVLGVTLAVRTAAGRPTFGHT
                     LFEGAAPGGLEGVTSWVAEVAQSALDIVLFIDEADRLPPSARDALAYLLRNAPPNLRV
                     VVAARADCHLQLDDLTDYGHCAVVSPDVLRFRLDETIALVQARFGARVDRDAIARLHD
                     LTEGWPLGLQLALSLMAAGSDPQAEVARLARQDSGLRDRFVSLLLTNLDPADAAFLTR
                     IAMLDDLHPSLCSAVVQADDAPERLARLARDTPVFVAGDASDWLRMHSLAREELRRRF
                     HNLPEAEQAELHRRAADWLGHHGLLEAAARHALAAGEADLAYELAERSLYESLMTRGR
                     QGAVLAWLARLPPTEVDRRPRLLLAAAWTLALSERHDEARRLVERLLAQPGVHDALRC
                     ECALILGGAAVFADEPDRFAQLHDPWSEHPPLRDALLLQVHANRSAFRALLEGEPALA
                     RLRLQQAPRNDLDPAAGYIGRWGELVTALTYLWEGQVLLAEKLLRPTLAGAEGDLGRR
                     SPFACMLASLLAAALWERNEPDEAAAVLANRLDVLERHGLPETVLLGFRTVARIAGAE
                     GAENRALELLAALEAVGVSRRLPRLRLASLVDQVRLHAHRFRADTCRGLCDRIDVLMA
                     DPTLPQGRLWQRQAAVLRDVAHAQAAIAARDWRGAIGPLKRGEALARELRLGRLKIEL
                     TGLLALALDRCGERSVGLLREAADLARAHGLQRVFADAHPTLGEWARQALAGSSLPDA
                     MATPVPVPAPARPAAAPRSTPSMALTPKEREVLELAARNLSNKEIGLAMEVGEETVKW
                     HMKNLFAKLDAGTRKQVVSRARILGLLAAEP"
     gene            complement(22151..>22297)
                     /locus_tag="PK399_04905"
     CDS             complement(22151..>22297)
                     /locus_tag="PK399_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPW12494.1"
                     /translation="AIQHLAGMKDSKVIVAINKDAEAPIFSVADYGLEADLFDAVPAL
                     TSAV"
BASE COUNT         3341 a         7633 c         7761 g         3562 t
ORIGIN      
        1 agtgcatcgc cggagggact gcagtctcgc gcatcaccgc ttcggtgctg cggtagcgtt
       61 cggccatggc gcgctcgtcg gcggtcagta gttgcgcctg catgtacgcg cccagcgcac
      121 gagcatgcgt gacgatcgat tcgccgaacc gcagccgttc ggcttcgtat gcccgcaacg
      181 cgccaggcac ctcggcatcg gccgagagcg ctcggacgag gctcatggcg tcgccagccg
      241 ccttcgtcac gcccatgccg cagtgcggcc gggcgacgaa cgccgcgtcg ccgagcagcg
      301 ccacacgtcc gaaggcaagg ctcgggcttt ccaggtcgaa gatcggctgg aagaacaact
      361 gctcggtctt gcagaccact tcggcgaact gcggggcaag cacctcgcgc gctgcctgcc
      421 gcgcaccgtg gagcacttcg ggccggatca gcggcggcgg gatgccttcc ggccaggtct
      481 tcccggtcgc gtcggtgacc atctcgcgca gggtgcttgc ctcgtccgcc ggccggtacc
      541 agacgaaatt gaaacggcgc cttcccggct cgaccgcgtg accgggaccc gcgaccgggt
      601 aggcgatcat ctgctcgcgt ggcggaagcc cgaaggcgaa gaagggacac agctctcgcc
      661 tcgtgcgttc gctcagtgcg gcctcgtcga ccaacccgcg ccaggcgacg tagcctgcgt
      721 agaccggcct gacctgcggc agcaggcgct ggcgcaccgt cgagcggatg ccgtcggccg
      781 cgaccagcaa ctcggcttcg gccgctgcgc cgttcgtgaa gcgggccacc actgcgtccg
      841 ccccttgctg cagcgcttcg agctgatggc cgttgtggta gcgatcagcc ggaaatgcag
      901 ccttgagaac ctggtacatc cgaccccacg cggtcagcac ctgcgcaagg gcatgacgcc
      961 cggcgaccga gccgtcgcgc gccagcgtga cgcgctcgct caccgtcacg ccgatgtcgg
     1021 tggcttccgg cacgccggcg gcgtgcagcg tgtcgatcag ctccgggtgc gtcacgatgc
     1081 ccgcgccacg accctcgagt tcttcggcca cgcgttcgta cacatgcacg tcccacccgg
     1141 cccggtgcag caggttggcg gcgaacagac cgccgagcga accgccgacg atgatgatcc
     1201 tgcgctgcga tgacatgttc gtctccttcg gctggtcgtg tcgtgtcgcg gctcagcacg
     1261 cggctgccgg gtggccggcg ggcttcgcgt tctcggcttc ggccgcgggg aagttcagtt
     1321 cgatggtgat tccggacggg tcctcgacga acacctggtg cagcccgagg tccggaaccg
     1381 tgcgttctcg cgtcggaagt cccgcccgtt gcaggcgacc ccgcatgtcc tccaggccgg
     1441 tggcgaggaa cgccaggtgg tcgaccgcgg cgctgccgtg caacgacgca gcgtccttgt
     1501 cgccgaggta gcggaccagc ccgtcggggt cgtcgcggtc gctgccgatc aggtgcacca
     1561 cgccgtagtc cgcctcgtcg ccgccgctgt acagccacag ccccgggaac tcgaacggcg
     1621 gccggtagcc ctcccggaaa ccaagcacct cggtgtagaa gcggcgcgag gcctcgatcg
     1681 aggtggtcct gaccgagaag tgggccagct tgttgatcgg catgagcgcc tccatgtggt
     1741 ttacaaatga tcgactgatc aatagtagtc gacttgcaac cagcccgcaa ggcctttctt
     1801 gccggtcctt gccggctgtg gttcagcgcc ggcgggcggc ctgcaggacg gccggcacgc
     1861 cgagcaggaa ggcgtcgacg cgctggtcga tcaggcggtc caggttcttt ggcgtgggca
     1921 gttcgtagat ccacttgcgc acgccgatgt agaagacgct ggagtgcatc gcccagacca
     1981 gctcgacctc gtcgtcttcc tcggctgcgc cggccggctc ggggatgccg aactcgtgcc
     2041 tcaactcgct cgcgatcagc aggaaatggc gctcgcgcaa ccgtgccagg tacctctggt
     2101 tgatgccgcc ccggctcagg ccggcataga tgaagatgcg cacccactcg ttgcgaagca
     2161 cgaagccggc gtagtcgcgc aggtagcgca ccagccgcgt ccgcaggggc acgctgcggt
     2221 cggcgagcca ttcctcccac tcgtggttcc agcgtcgcac gaagacttcc tcgtagaccc
     2281 ggtcgaccag ggcctccttg gttgggaagt agcggtacag cagcggctgc gtgatgttca
     2341 gctcgcgtgc caggtcgcgc gtgctggcct cgaagccgtg gcgcgcgaag aagccgatgg
     2401 cggcctgcag gatctgatgc tcccggtctt cgggggcgag gcgccggcgg ggcgcgccac
     2461 ctgcgacaga tgagacagct gcggcatcgc ggtcgcgatg ggcgttggag accgacctgg
     2521 aggccatggc gttaccgggt ttacccgact ctatttatca attgacaaat aggcctgggt
     2581 gcatcaaagt gatcgaacga taaacagcat cttagccccg tacggcgcgc cgcgccactg
     2641 gagaccgcca tgaaatctgc gaagctcgct tcgacgctcc tcccccgagc cgtgtctgcc
     2701 gtcgcgctgg cctccgcggc cgtgctgtcg cacgcgcaga ccatcaagat cggcttgccg
     2761 gtcccgctga ccggccccta tggtgccgaa gccaaggacc aggtccgcaa tgccgaactg
     2821 gccgtgaagc agttcaacga cgccggcggg ctgcacggcc gcaaggctga acttctggtg
     2881 cgcgacgaca agctcaaccc cggcgaggcc gcgacgcgca cgctcgagct gatcgagaag
     2941 gacggtgcgc agttcatcgt cggcggcctg tcggccgcga ctcagttgtc gatcaacaac
     3001 gtgacccgcc agcgcaaggt gatctacgtc tcgatcagcc agtcggacgt catcaacgag
     3061 ccgcccgacg ccagtccgta caccttccac gaggcgctga atccatacat gacgacacag
     3121 gccgtcggcc gtcacgtgtt caagaagggc atgaagatcg cgttcctgac tgccgactac
     3181 gcctacggcc acgagatgac ccgcggcttc acgcgcgtgg cccgggacgt aggagcggag
     3241 gtcgtcggcg aggtgaagca cccgctgggc gcgcaggact actcgacgtt cctgccgcgc
     3301 ttgaaggcgc tgaatcccga cgtgctggtg ttcaacaact tcggcgccga caaccggatc
     3361 gcgatcaagc aggccgggga cttcggcatg aagaaggcga tgaagttcgt cacgccgatc
     3421 ctcacttaca cggcccgcgt cgccggtggc gcgggcgtct acgaaggcgt ggttggcggc
     3481 acgcagtact actgggaact gcaggacaac acgccttcgg ccaaggcttt caacgccgcg
     3541 ttccaggccg ccaacggcgg gcgctacccg tccgactacg gtgcgatggc gtactcggcg
     3601 gtgaagtcgc tgttggcggc agtcaggacc gccggcggca ccgagaccga caaggtggcc
     3661 caggccctgc ggtcggcgcc ctacgacagc tacaagggcc gccagttcta ccgcgcttgc
     3721 gaccagcagt cggtgcagtc ggtgctgctc gtcgagtcga acgccaagga tgcagcccat
     3781 cccgacgacg tgttcaagct gctgcacgtg gagccggcca gcgaatccct gcttcgctcc
     3841 tgccaggaac tgggcttcaa gggctgaggg cgccaccatg aacctcactg tcgacctgct
     3901 ggcactgcaa cttttcactg gcgttgcact gggcgccatc tatgtgctcc tggccgtcgg
     3961 gctgtcgctg atcttcggca tgctgacggt cgtcaatttc gcccatggcg ccttcttcat
     4021 gctgggcgcc tatgccggcg tgtggctggc cgaccgcacg ggcagcttct ggtgggcctt
     4081 gctgctggcg ccgcccctcg tcgcggcgct cggcatgctc tgcgagcggt ggctgatccg
     4141 tccgctctac ggccgcggca tcgactaccc gctgctgctg accttcggtc tggcatacat
     4201 gatggtcgag gccgtgcgca tggtgttcgg caaggcggcg ctgccgttcg atacgccgga
     4261 ggcgctgcaa ggcgcgacga gtctggggtt caccgacttc ccgacctatc gcctcttcgt
     4321 gatcgccgtg gtggtggcgc tgctgctggc gctgtggctg ctgatcgaga agaccccgtt
     4381 cggcctgatc gtgcgggccg gcgcgcgcga tccggtgatc gtgcgcgtgc tcggcgtgga
     4441 cgtctcgcgt gtgtggctcg tcgtcttcgg cgtcggcacc ggcatcgcgg cgctggccgg
     4501 cgtgctctcg gctccgctcc agggcgtgtc gcccgagatg ggcacctccg tgctgaccgt
     4561 cgcattcgtg gtcacggtgg tcggcggcat ggggtcgctg atgggcgcgg tcgtggcggg
     4621 cctgctcatc ggcatcgtcg agagcatggc ggtgctgttc ttcccggagg ccgccaaggt
     4681 ctcgatgttc gtgatcatgg ccgccgtgct gctgctgcgc ccgcaagggc tgttcggccg
     4741 cgccggcctg ttggcctgaa ggagtcgatc gtgatgagtt ccgccctcaa gcgcacggtg
     4801 ccggcgtcgg cgcacaagat ggatcgctgg ctgcagcagg tgggtctgca ccgggtggcc
     4861 gcgaccgtgc tgatcttcgc ctgcctcccc tgggtgatgc cgtacccgtc gctggcgatc
     4921 aacatcctga tctacggcct ctacgctgtc ggcttcaacc tgctcttcgg gtacaccggc
     4981 ctgctgtcgt tcggccatgc cgcgttcctg ggcgtcggcg cctacaccgc cgggatcctg
     5041 atcgcccagc atggcgcgtc ctggtggtgg gccattccgg cggccgtcgc gatggccgca
     5101 ctggtggccc tggcgatggg gctgctggcc atccggacgc gaggaatcta cttcgcgatg
     5161 gtcacgctgg cgctgtcgca gtgcgtgtat ttcatcgtct accaattgcc ggcatcgggt
     5221 ggcgagaacg gcctgcgcgg cgtcaacctg gccgaagtgt cggtcgccgg tctgaaactg
     5281 aacctgctgg atccgatgca gaagtactac ttcgtcgtcg tcttcgtcgc ggcggcgatg
     5341 ttcgtgctgt cgcggatcct ggcctcgccc ttcggcggcg tgatcgaggc ggtgcgcgaa
     5401 aacgaatcgc gcgcgcgggc ctgcggcttc gacatcgagc gcaccaagct gctggccttc
     5461 gtgctctcgg cggccttctg cggcctggcc ggggcgctca acgccatcca cctgtcgagc
     5521 gtgccgatcg agacgctgca ctactccacc tcgggcatgg tcgtgatgct ctgcctgctg
     5581 ggcggcatgg gcacctactt cggcccgttc gtcggcgccg cactgttcct gggggtccag
     5641 gacctggcct cggcgtacac cgagaactgg cagctgtacg tcggagccct cttcgtgctc
     5701 ttcgtgctct tcttccccaa gggtgtttgg ggcagcttcc tcgaaaggat tcgccatggc
     5761 cgttgacacc atcatcgaga cacgcggcgt cagcaagcgc ttcggtgcct tcaccgcgct
     5821 caaggatgtc agcgtgaagg tgccccgggg caggctcacc gccatcatcg gcccgaacgg
     5881 cgccggcaag agcacctact tcaacgtgct gtccggcgcc ttcgcaccca gcgaaggccg
     5941 ggtgctgttc gatggccgcg acgtgacggg aatgaagccg catgactatg cccacaccgg
     6001 catcgcgaag agcttccaga tcacgaacct gttccccagc ttctcggtcc tggagaacgt
     6061 ccgcatcgca ctgcaggccc acgtttcgcg ctacggcctg tggcgcagtc gggccgcgct
     6121 gcaaggtctg caggaccagg cgatggggct gctgctgacg gtggggctgg cggactgcgc
     6181 gaagcgcctc gcgcgggagc tggcccatgg gcagcagcgc gcactcgaga tcgcggtcgc
     6241 actggccgcg cgcccgaagc tgctgttgat ggacgaaccg accgcaggca tgagccccga
     6301 ggagacgaaa gtcatgatgg ggctcatacg ccgcctcgtg gccacccaca ccgtcgtgct
     6361 ggtcgagcac aagatgaaga tggtgatggg catcagcgac cacatcgtgg tgctgcacca
     6421 cggcgaactg ctggccgaag gccgccccga agacatccga cgcgacgaga tggtcaagat
     6481 ggtctacctg ggccagggcc agcactgatg caagcactga aggagcctgc aatgctgcaa
     6541 gtcaagggtc tgcacgcttg gtacgggacc agccacgtgc tgcagggcat cgacctggag
     6601 gtcgcggccg gggaactggt gtgcctggtc ggccgcaacg gggccggcaa gacgacgacg
     6661 gtgaagagcg tcgcaggtct gctgccgaag gtgcagggct cggtcgtgtt cgagggccgg
     6721 gagattctgg gattgccggc ccatgaccgc ttcggcctcg ggctggccta cgtgcccgag
     6781 gagcggcgca tcgtcccggg cttgaccgtg cgcgagaaca tccggctcgg cttgctggcc
     6841 tcgccgctgc gccggcgcga agcggacgtc atcgacgagc tggcgcagac gtttccgagg
     6901 ttgaaggaac gcctggacca gcaggccatc acgatgtcgg gcggcgagca gcagatgctg
     6961 gccatcgcgc gcgcggtcgc ttccaagccg aagttcgtgc tgctcgacga gccgtcggaa
     7021 gggatcatgc cgctgctggt ggaagagatg ttccagtact tcacggcgct caagcgcagt
     7081 ggcgtcacga tcctgctgat cgagcagaac gtcgagctcg cattgcgcat cgccgatcgg
     7141 gcctacatcc tcgaccaggg ccaggtcgtc tacaccggtg cggccgacga gatgctggcg
     7201 gacgaggcga tccaggagcg ctactgcgcc gtgtaagccg cccgtgggcg ctcaggggct
     7261 gaccgcggcc ggcgccaagc gccgcatcgc gtcgatgaac cggctcaagc gcggggcata
     7321 gatccggctg tgcgggtaga cgaggtggac cggcaacggt gcggcttccc atccgggcac
     7381 cagatgcacc aggtgtcccg cctcgatctc gctccgcacc agccagctcg acgagatgca
     7441 cgcgcccaga cctctgacgg ctgcgctgcg tagcgcgtac aggctgtcgg tgctgaagcg
     7501 gggccggatg gggaaggcga ccaccgtctc gtccacgagc gaatgcagcg cgacctcatc
     7561 gcggtagaaa gtccgcagcg ccagccaagg catcgacgcc agatcttgcg gatgcgatgg
     7621 gacctgcctt cccagcaagg ccggcgcggc aaccgcaatg cgccgcactt cgccgagctt
     7681 gagcgcgacg acgttcggat catcgaccac gccgacctgg atggcgcagt cgaccccttc
     7741 cgcgatgaaa tcgggcctgc ggtcgtggag cagccattcg acggtgacgc cagggtactg
     7801 gcgcaggtag tcggtgacgg ggccgatcag ttgatcctgg ccgaaggcgt gaggagccac
     7861 cacacgcagc gtgccctcgg gatgcgcggc cgcgcctcgc aggtcggcgt ccagggcggt
     7921 ccaggtctcc agcaggcccc gggccagggc aaggcagcgc tcgccgtcct ccgtgagctt
     7981 catcacgtgg gtcgagcgct gcagcaggcg caggcccagg tgttcctcca ggcccttcaa
     8041 gcgccggctg atcgtcggct ggctggtgcc caactgggcg gcagcggccg agaggctgcc
     8101 cgcgtcgacg atgcgaacga aggtctcgaa cagttccagg cggtcggagc tgcggcccgg
     8161 cttcagtcct tgcatacgga aattgtatat cgcttctgcg gcgcaggcgg ctaccacgct
     8221 tgcgaggagg cccctacagt gacggcaacg taccaaccgg gaacccgctc atgtcgaata
     8281 ttcactcagt gcatacaaac ggcacgcatc catcgccttc aagggtcgcc gcccggacgc
     8341 ttccgatgct ggcggtgagc gccggcttca gcgtcgcggc gctctactac gcccagccca
     8401 tgctcggcgt agtgggcgcg actttcgatg ccggctcgca ggccttgggg ctggtgccta
     8461 cgctcaccca actgggctat gccgcgggca tcctgctgct ggcgccgctg ggcgaccgct
     8521 tcgatcgcaa gcgcatcatc ttgaccaagg gcttcgtgtt gacgctggcg ctcgtgctga
     8581 gcgccatcgc gccgtcgctg gcctggttgt tgggcgccag cctggtcgtc gggctgtcgg
     8641 ccacggtcgc ccaggacatc gtgcccgccg cagcgatact tgcgccggag accagccgtg
     8701 gcaaggccgt cggaacggtc atgacgggcc tgctgctggg catcctgctg tcacgcgtgg
     8761 tgagcgggtt cgtctcggag caactgggct ggcgatcggt cttcatcgtc gctggcgcgg
     8821 gcatggcggt catcgtggcc ctcctttggc gctgcttgcc gaacttcaag ccgaccacca
     8881 ccgtgcgcta cggcgcactg atggagtcga tgctggcgct gtggcgacgg catgcgtccc
     8941 tgcgccgcgc cgcttggtcg caaggcctct tgtccgtcgg gttcagtgcg ttctggtcca
     9001 cgttggcgat cctgttgcac ggcgagccgt tccatctggg cagcgctgcc gccggcgcgt
     9061 tcggactggc gggcgtggct ggcgcgctgg cagccccgtt ggccgggcgc atgtctgacc
     9121 gcagaggtcc ggaagtcgtc acgcgatggg gtgcttctgt cgccgccgtg tcgttcgctg
     9181 caatggcact gacgccgctc ctgtctccca cggcacagct gtggctgatc gccgcgtcgg
     9241 ccatcggttt cgacttcggc gtgcaagctg cgctcgtggc ccatcagacg atcgtctatg
     9301 gcatcgagcc cgccgcccgc agtcgactga atgccgtgct gttcgctggc atgtttgcgg
     9361 gcatggccgc cggcgctggg ctcggcagcg cgttgttcgc ccattggggc tggatcggcg
     9421 ttgtcggact tgcctgcgct tcggccatgg gcgcgagcgt cattcggatg ctgccgatgc
     9481 atcaccttca actggcgtcg caggcccggt aggccccgct gcatgaggca agcggcacgt
     9541 cgcgaggcgg cggggcattc agcgctctcg caaggtgtcg aacagcgcga gcgccgcgcg
     9601 cacccgcggg ggcacgaggc gcccggacgg catgaccgcg ctgatcggca tcggcgccgg
     9661 gcggcacgac ggcagcagtt ccaccacctc accggtcgcg accagccgct cgacgaagta
     9721 gtgcggcacc tgggcgatgc ccaggcccgc acgcacggcc tgcacgatgc cgtcgccatc
     9781 gttgaccgag acccgcgaag gcggatgcag cgtcaccggc tgcccgcgcg agcgcagctg
     9841 ccagggccgg tgcttgccgg tgctcggcag gcggaacatc acgccgtggt ggcccgacaa
     9901 ctgctcgagg gtgcgcggcg tgccgtgccg ctcgagatag gccggcgcgg cgcacagcac
     9961 cagcgcctga ctggcgaagc gccgcgcgac gaggctggaa tcccgcaggt cgccgacccg
    10021 cacgacgagg tcgagcccct ccttgaccgg gtcggcgtaa ccatcttgca gccgcacgtc
    10081 gagctgcagg cccgggtgct tgtccagaag cgtgccgagc agcggcagca gcacctcgcg
    10141 gccgaagacg atcggcatgt cgatgcgcag cgtgccggtc ggctccatgc gtgcgccagc
    10201 cgcctcgacg tgcaggtcct cgacctcggc gagcacccgc tggcagcggt ggaacagccg
    10261 ctcgccgtcg gtcgtcaggc tcacctgccg cgtcgtgcga tggaacagct tgaccgccag
    10321 cgacgtctcc aggcgcgaca ccgccttggc gagcgtcgat gacgaggtgc cgagctcgcg
    10381 cgcggcggcg gcgaagctgc cgtgcttggc ggtctcggca aatgcgagca gctgctgcag
    10441 tcccttcatc gcggcgccat ccattacgga agaagtgtcg gcaatgttag tccgttgacg
    10501 cctcttccgc cgcagcctcg cggtcggaat actgcgaagc gtccgaggcg ccgcggacgg
    10561 ttcatcaccc atcctccccc agcacattgg agcagccatg tacgtccacg accagacctc
    10621 ccccgccccg accgcgctcc ccggcatcgc ccacgccacc tgggccggca ttgccgagaa
    10681 cgtcggcctc agcctgtggc gccagaccgt tgcgcccggc gccggcacgc cgccgcatcg
    10741 ccacgactgc gacgaggtcg tgctgtgcac cgccggctac ggcgaactgc acatcgccgg
    10801 ccaggtccag cgcttcggac ccgaccagac cctgacgctg ccccgtggcg tcgagcacca
    10861 gttcttcaac gtcggtgcct tgccgctgga ggtgctcggc atctttgcgg cctcgccggt
    10921 gggcacgttc ctgcccgacg gccaggcgat cgacctgccg tggcgctcct gagcgccgca
    10981 tctcttcaac ccctggactg gagacaccca tgagcaactg gctgcttcgc atcgcgacgc
    11041 tctacctcgc cgccggcgtc acgctcggca tcgccatggc cgcgagccac aaccacgtcg
    11101 aggcaccgct gcatgcgcac ctcaacctgc tcggcttcgt gacgctcacg ctcgcggcgc
    11161 tctggtaccg cgcccagccc gaggcttcgg acacgacgct cgcgaagatg cacttcgtgc
    11221 tgcacaacct cggcctgccg gtgatgagcg gcggcctgtt cttcctgctg cgcggggagc
    11281 cggctgccga gccggtcgtc gcgatcggct cgacggtcgt ggcagcggga atcgcctgct
    11341 tcgtcgtcaa cctgtggcga tgcacgggac accgcaagtc cacgctcgat gccctcgcga
    11401 ccgcgctgcg ctagttcgtg atcagcagat ggaccgtcgc cacctgggcc gaatcgtagg
    11461 tggacgtcgc caccagagtg atcgggatcg acgcgggaag cgggtacgca gcatacgtgt
    11521 cgaccgcgac actggtccgg ctcacggtcg tgcctgtgat gtcggcgccc gcgtagtaca
    11581 cctgggcgcc gctgccaacg gcagtccctc cgacgtacat cgtccagacg acgggctcgc
    11641 ccgcatccag gatcacggac tggccggcac gcaccgcgag atccagcgat ccgcccgaca
    11701 cgaccggcgt gtcgttgacg aactggcctg cgacggtgat gccgatgtcg aaggtgacat
    11761 tgcctgccga atcgacgtag gcgccacccc cacagccact caacgaccag gccgtcgcaa
    11821 tggccgcgac cagcgcgatc catcgcttgc tgcgcgggcg ggcgatcaaa ttcaaggagc
    11881 gatcttcagt cgtcatctcg tgggcgtggg tcatggacgg cctcggctac gccgttgggg
    11941 gacgcagcgt tttcaggtgg accttcaacg cgttcgagtc cacatccgat gacgaagggg
    12001 ccggccctta catggcctca catttcgagc ccgtccgtgc cgctgctgcc gatgcgcacc
    12061 tgcagcttcg gccccccgca ctcgaggacc aaggggcagc gccatccgga atcgcgcgtc
    12121 cggagcgcgc cttgatccat gaggcccggt gcgtccacca gcttccgggc tcggactcag
    12181 ccgtcgccag ccacagatcg gggtcaccgt gggcctgccc cctggccagt ggctccgctt
    12241 gttccgcaat aggccgtgtt gcggcagccg atcagcgtgc ggcccgtccc cttcgttcgg
    12301 cgctggtgag ttccacgcgc atcacgatcc gcgaaccgga cgccactgga cccactggtc
    12361 gtcgcggctg cagtcggcaa agcggtcgat caagggctga tcgacccgca ccgcacgggt
    12421 catgccgaag tcgcggctga caggcccggg cacggtgtcg acgcggtcgt cagaagacgt
    12481 caggtgtcct ctcgcgtggg gtcaggcggt gggcagcggg tagtcggcca gcagctcgcg
    12541 cagccggttc ttctgcatct tgccggtcgc tcccagcggg atcgcgtcga cgaagaccac
    12601 gtcgtcgggc gtccaccact tggcgatctt gccgtcgtag aacgcgagca gttcgtcgcg
    12661 cgtgacggtg ctgccggact tcctgaccac gaccagcaac ggccgctcgt cccacttcgg
    12721 atgcttcgcc ccgatgcagg cggccatcgc cacgcccggg tgggccatcg cgatgttctc
    12781 gaggtcgatc gagccgatcc actcgccgcc ggacttgatg acgtccttgc tgcggtcggt
    12841 gatctgcatg tagccgtcgg cgtcgatggt ggagacgtca ccggtgggga accacccgcc
    12901 gatcagcgga tctccgcctt cgcccttgaa gtaactctgg atgatccagg gcccacgcac
    12961 catcagttcg ccggacgcct tgccgtcgtg cgccagcttg cggccatcct cgcccacgat
    13021 cttcagctcg acgccgtaca ccgggcggcc ctgcttggac tgcaccgcca tccgctcgtc
    13081 ggcgcccagg tccgcatgct tcggcttgag cgtgcacacg gtgccgatgg ggctcatctc
    13141 cgtcatcccc caggcgtgca gcacctgcac gtcgtagcgc tcctggaagg cgcgcatcat
    13201 cgcgggcggg caggcggcgc cgccgatcac ggtgcggcgc atcgtgccga agccgagctg
    13261 gttcgcttcc acgtgcgcga gcaacccttg ccagacggtc ggcacgccgg ccgacatcgt
    13321 gacgcgctcg ccctcgaaca gttcgtgcag gctcctgccg tcgaggaacg ggccgggaaa
    13381 aacgagcttg gcgccgacca tgcaagcgac gtacggcaag ccccaggcgt tgacgtggaa
    13441 catcggcacg acgggcagga tcacgtcgcg cgcgctgcag ttcagcgcgt cgggcagcgc
    13501 ggcagcatag gtgtgcagca ccgtcgaccg atggctgtag agcgcaccct tcgggttgcc
    13561 ggtggtgccc gacgtgtagc acagcgagga cgcggtgttc tcgtcgagct gcggccaggc
    13621 aaacgcgtcc ggcgcgtcgg cgatcagttc ctcgtagcac agcaggttcg gtaccgtgct
    13681 ggtggcgggc atgtgcgcgc ggtcggtcat cgcaacgaag gtcttgatcg tcttcacgcg
    13741 cggggcgacg gcctcgatca gcggcaggaa ggtcaggtcg aagaacagca cctgatcctc
    13801 ggcatggtcg gcgatccaca ccacctggtc gggatgcagg cgggggttca gggtgtgcag
    13861 caccgaaccg gagccgctga ccgcgtagta cagctccatg tggcggtagc cgttccatgc
    13921 cagcgtcgcg acgcgcgagc cgacaggaac ccgcagcgcc gccagcgcgt tggccatgcg
    13981 gcgggcccgt gcggccagct cgcggtaggt ggtgcggtgc aggtcgcctt ccacgcggcg
    14041 cgagacgatt tcctggtcgc cgtgctgcgt ctgcgcatgc ttgagcaggg aggaaatgag
    14101 cagcggctgc tgcatcatga gtccgttcat ggtccttgtg tccttcaaga gggatcgtcg
    14161 gatcacgcga gcgcgctgcg catcgcggtg gcaggttcag gcgcgagcgg ctcggccgcg
    14221 cgcgccttgt cgctctcgta gctgcgcgcg gccatcaggc aggcgcccgc ggcgagcagc
    14281 gcgcacagcg gaatcacggt gagcgccggt accagccccc aggcgtccga cagcaggccg
    14341 gccacgaagg gccccgccgc gaggccgaac aggttctgga agagggccag cacggacgct
    14401 ccggtcgccc gcacaccggg gtggatgacg tcgatgacga tcgccgacac cggtccgacc
    14461 gtgcaggtgg ccacgaagcc gccgatggcg atcagcgcga actgggccgg cggcgcaagc
    14521 gacgtgccga aggccgtgac gagcaggacc atcgacagca cgcacagcac cgccatcgtg
    14581 acgagcttgc ggcgcggatg acggacgccg acgcgatcga ccagcgtgcc ccagaccacg
    14641 gagcccaggg ctccggccag gaccacgagc gcggccttca cgcccgcctg ggccggcgcg
    14701 acgccgtgca cgcggttcag gaagctgggc agccacgacc acagcgccga cacgacgatc
    14761 agctgcgccg cgccaccgag gcagacccac agcatcgtgc gcgtccgggc cagcacccgg
    14821 acgatgtggc gcaccgcctc gccgatcgaa cggcgcttgg catccagcgc cggttcgagc
    14881 gacacggtgc ggtagtcgcg aaccttcagg taggccagcg ccaccaggag gccgggcacg
    14941 ccgacgacgc cgaacgccgc ctgccagccc cagcgctcgg cgatcatgcc gccgagcatc
    15001 acgcccagca ccgagccgac cgaggcggac gcgaagaatc cggccatcag cgcgccgcgc
    15061 atgcgcgccg ggaagtggct ggcgatcagc gccgccccca ccgagccgta gccggcctcg
    15121 cccaggccga cgacggcgcg cgccgccagc aactggccgt agccgcgtgt ccacatgcac
    15181 gagatcgtgg ccaggctcca cacggtggcc atcgcgacga tgctcttcac gcggctgacg
    15241 cgatcggcga acagcgccac cggcaaggcg ccgagggcga cggtcaccga caccaccgac
    15301 accagcgcgc cgagctgctt gtccgacagg ttccattccg ccttgaggta ggggaacagc
    15361 gagacgatca cctggcgatc gacgtagtcg aagaccatca gcgcgagcgt catgccgaag
    15421 gcaaaccagg cctggccgcg gctgaagaga tagccgtcct gggccggagt cttttcgatg
    15481 ctgtgcaggt ccatggtggc ttccttcagg gaacgagcac ggtggcgccg gtggtccggc
    15541 gcgactcgag tgcgcggtgg gcctcggcgg cgtcggccag cgcgaactgc tggttcggct
    15601 cgccgcggat gcgtccggcc aggacatggc cgaacagctc gtcggccatg gccagcatgt
    15661 gggagcgggg cgtcgcgtaa tggatcatgg ccgggcgcgt gacccagagc gaacccttga
    15721 ccgcgagcaa ggtggtgtcg atcaccaccg gcccggacgt ggtgccgttg ctcaccagcg
    15781 tgccgcgcgg ctgaaggctg tcgagcgagg ccatcagcgt gtccttcccc accgagtcat
    15841 agaccacggg aacgcccttg ccgtcggtca gttcgcgcac ccgcttggcg atgtcttcgc
    15901 ggctggtgac gatggtgtgc gtgcagccgt gggcgcgcgc gatctcggcc ttctcgtcgg
    15961 tgctgacggt cccgatcatc gtgaccccca gcgcccgggc ccactggcag gcgaccagcc
    16021 ccacgccgcc ggcggcggcg tggtagagga tcgtctcgcc gccctggagc ggatagacct
    16081 gccggaacag gtactgcgcc gtcagcccct tcatcatcag cgtcgacgcg gtgcggtcgc
    16141 tgatgccttc aggcagcggg atcagcacgt cggccggcat cacgcgcacc tcggcatagg
    16201 cgccctgcgg gccgagcagg tagccgacac ggtcgcccac ctggatgtcc gtgacgccgg
    16261 cgcccaccgc ttccaccacg cccacggcgt ccgagcccag gccgttcggc agcggcagcg
    16321 gatagcggcc ggtgcggaag tagatgtcga tgaagttgac ggcgatgtag ctgtggcgca
    16381 cacgcgcctg gccgggcccg gggtcgccga catcgacggt ctcgagctgg agcacttcgg
    16441 gtccaccggt ctggtgtagg cgtatcgcac gggtcatggc gggtctcctg ccgaggcgcg
    16501 tggctgcgct cggcgatgtc tgtggggggg cgcggatgcc gcggttgtca ggcggcgcgc
    16561 ctgtgggccg cgtcgccggc gcaaagctcg tcgatgtagt cgttcgcctg cggccaggtg
    16621 ccgaagcccg aggccgggtt gaggtggccg acacgaccga ggtcgtcgag ccggctgccc
    16681 caggcgccgg ccagggcggc gatgcgctcg aagcgcccga gttcgtcgtt gcggctggcg
    16741 gcgacgatgc tgcggaacgg cagccggcag cgcggcaccg gcagccatcc gccggcctgc
    16801 agcgcggcca gcgtcggata gccggccggc agcggcgcgt cgaaatccgg cggcgcggcg
    16861 agcagggcac cggccaccac gcagcgtgtc tgctgcgccc agtgcgccac catgatgcag
    16921 ccggcgctgt gcgcgacgac gacgagtggc ccctgcacgg cctgggcctg ctgctcgatg
    16981 gcggcgacgc gggccgcgca gtcgaggtcc gcgcggccca tcggctgcac cacggcgacc
    17041 tggcggcgcg ccgcgcgcag ccgcatctcg agcagcgtct gccagtgctc ggcgacggcg
    17101 tcgcgcagcc ccggcacgat caggatgcag ggttcgttcg acatggcgtc aggccgcctt
    17161 gcgcaggtaa ccctgcaggc cgggccgtgc gttgggggcg aagccgttgt gcgccagcgt
    17221 ctcctcgacg ctgtcgtaga agacgccgat ctggcagatc tcgcgcgctt ccttgccggt
    17281 ggcgacctcg cggccgaact cgcgcgacag gcgcaccagc tgctcgatct gccgcacggt
    17341 gcccatcttg gccttgcggt cctgcgtcca gaggttgtcc tcgatgccgc agcgcacgtg
    17401 caggcccatg gccaggccca tcatgttgat cggcagcacg ttgcgcatgc tgctctcgac
    17461 ggtcagcatc gcgccgtccg ggatcgcgcg caggaagttg gcgaggttgt agacgttggg
    17521 cgcgtccatg ccgccgctga tcgcgaccca gttcatcacc agcgggccct tgtagacgcc
    17581 gcggcggatc atgcgctcga cggtctcgaa actgttgatg ttgtagaact ggaaggcgct
    17641 ctggatgccg gccgcgctca gccgccggat gtgctcctcg acccaggccg ggcccgacgg
    17701 cacgatcatc tcgctgtagg cgcgccggcc ttcgggcgtg gcgagcgtgg tgccggcgat
    17761 gtcggcctcg tccatgtgtt ccaggacgtt catctgcgtg gtgttgacgg tgacggtgac
    17821 ctgatccggc ttcgggtcca gctcggcgag catgtgccgc gtgtcgtcgg acagccactt
    17881 cgctgcggcg ccatcggact ccggcgcgaa ggagatcgag ccgccgacct ggatgatcat
    17941 gtccggcacc gcggcgcgca caccggcgat cagctcgttg aacttggaca gccgcttgct
    18001 gccccggccg tcgagctcgc gcacgtgcag gtgcagcacg gtggcgccgg cgttgtagca
    18061 gtccaccgcc ttctgcacct gctcctccat cgtcaccgga atgtcttccg gaaagtccga
    18121 gggcatccat tcggggccgt agggcgcggc ggtgatcacc agcttgtcct ggttttcggg
    18181 gaagagcgaa ccgtcgagga agttcatggc gggctttcgt gtcgtgtgac tcggctggtg
    18241 gattcagaac ggcttgtcgc cgacgatgcc ggcgcgttcc atctttcggt ggcagagcgg
    18301 gtagtccatc accgcgtagt gctgggtgct gcggttgtcc cagatggcga cgctgttcgg
    18361 ctgccagcgc cagcgcacct ggtactcggg gatctgcgcc tggctgatca ggtactgcag
    18421 gagctgcgag ctgccctgcg tgtagtcctg cccgacgcgc acgcgctccg gggtgtggaa
    18481 gttcgtgaag tgggtcgtga aggcgttgac gaacaggacc ttctcgcccg tctcgggatg
    18541 cgtgcgcacc accgggtgct cggcatccgg aaactgcgcc ttcagcgcga ggcgccgctc
    18601 gatcggcatc gcggcgccga aggtggcctc gatgctgtgc cgggcgcgca ggtcggcgat
    18661 ctgcgccttc accgactccg gcaggcggtc gtgggcgagg accatgttgg cccacatcgt
    18721 gtcgccgccg accggcgggc actcgacgca acgcagcacg cagccgaagg gcgggcgctc
    18781 ccgccatgtg gcgtccgagt gccaggcgtt ctcgtaccgg tcgttcggct gctcgggtga
    18841 cttgtagatg cgcacgaggc ccgggtgctc cgggtcgctg ccggccaccg ggtggtcttc
    18901 cagatcgccg aagtggcggg cgaaggcgac atgctcggcg cgggtgatgt cctggtcgcg
    18961 gaagaacagc acccggtgct tcagcaacag cgcgcggatc tcggcgacaa ggccggcatc
    19021 gcgtgacgcg acgccgaggt gcacgccgct cagctcggcg ccgatcgcgc aggtcagggg
    19081 ctggacccgg atcgagtcgg gcagcgaagc cgctcgaaca atggcgggtg attcggggcg
    19141 gggggtcgtc atgcgcttgt ctccttcgac gctgctcggc ggcagccatc ttcggaagca
    19201 agagtaggcc gtccgcccct tggccgcccc cccgtcgcag aggggggcac ggtcttcact
    19261 gtcctaaggt tcggcggcca gcaagcccag gatgcgcgca cgcgacacca cctgcttgcg
    19321 cgttccggcg tcgagcttcg cgaacaggtt cttcatgtgc cacttcaccg tctcttcgcc
    19381 gacctccatg gccaggccga tctccttgtt ggacaggttg cgcgccgcca gctccagcac
    19441 ctcgcgctcc ttcggcgtca gcgccatgct gggcgtcgag cggggcgccg cggcggggcg
    19501 agccggcgcc ggcaccggca ccggggtcgc catcgcatcc ggcaggctcg agcccgccag
    19561 cgcctgccgc gcccattcgc ccagggtcgg atgcgcatcg gcgaacacgc gctgcaggcc
    19621 atgggcccgt gccaggtcgg ccgcttcccg cagcaggccg acggaccgct cgccgcagcg
    19681 gtcgagtgcc agcgcgagca ggccggtcag ctcgatcttc agtcggccca gcctgagctc
    19741 ccgggcgagc gcctcgccgc gcttcaacgg tccgatcgcg ccgcgccagt cgcgcgcggc
    19801 gatcgccgcc tgggcgtggg cgacgtcccg aagtaccgcg gcctggcgtt gccagaggcg
    19861 cccctggggc agggtcggat cggccatcag cacgtcgatc cggtcgcaca ggccacgaca
    19921 ggtgtcggca cggaagcggt gcgcatgcag ccgcacctgg tcgacgaggc tggccaggcg
    19981 caggcgcggc aggcggcgcg aaacgccgac cgcctccagc gcagccagga gttcgagcgc
    20041 gcggttttcg gcgccttcgg ccccggcgat gcgcgccacc gtgcggaagc ccagcagcac
    20101 cgtctcgggc aggccatggc gctccagcac atcgagccgg ttcgcgagca ccgccgcggc
    20161 ctcgtccggc tcgttgcgtt cccacagtgc cgccgcgagc aggctggcca gcatgcaggc
    20221 gaacgggctg cgccggccga ggtcgccttc ggcgcccgcc aaggtcggcc gaagcagctt
    20281 ctcggccagg aggacctggc cctcccagag gtaggtgagc gccgtcacga gttcgcccca
    20341 gcggccgatg tagccggcgg ccggatcgag atcgttgcgc ggtgcctgtt gcagccgcaa
    20401 ccgcgccagc gccggttcgc cttcgagcag ggcccggaac gccgagcggt tggcatggac
    20461 ctgcagcagc agcgcatcgc gcaatggcgg atgttcgctc cacggatcat gcagttgggc
    20521 gaaacggtcc ggttcgtcgg cgaacacggc cgcaccgccg aggatcagcg cgcattcgca
    20581 gcgcagcgcg tcgtgcaccc cgggctgcgc gagcaggcgc tcgaccagcc gacgggcctc
    20641 gtcgtggcgc tcgctgagcg cgagcgtcca cgcggccgcc agcagcaggc gcggtctgcg
    20701 gtccacttcc gtgggcggca gccgcgccag ccatgcgagc accgcaccct ggcgaccgcg
    20761 cgtcatcaag gattcgtaga ggctgcgctc ggccagctcg taggccaggt cggcctcgcc
    20821 ggccgcgagc gcatggcgcg cggccgcctc gagcaggcca tggtggccca gccagtcggc
    20881 cgcacgacgg tgcagctccg cctgttcggc ctccggcagg ttgtgaaagc gcctgcgcaa
    20941 ttcctcgcgc gccagggaat gcatgcgcag ccagtcgctc gcatcgccgg ccacgaacac
    21001 cggcgtgtcg cgcgccaggc gcgccaggcg ctcgggtgca tcgtcggcct gcacgaccgc
    21061 gctgcacagc gacgggtgga ggtcgtcgag catcgcgatg cgggtgagga aggccgcgtc
    21121 ggccgggtcg aggttcgtca gcaacaggct cacgaagcgg tcgcgcagcc cgctgtcctg
    21181 gcgagccagg cgcgcgacct ccgcctgcgg gtcgctgccg gcggccatca gcgacagcgc
    21241 cagttgcagg cccagcggcc atccttcggt caggtcgtgc aggcgtgcga tggcgtcccg
    21301 atcgacacgc gcaccgaagc gcgcctgcac cagcgcgatc gtctcgtcga gccggaagcg
    21361 cagcacgtcg ggcgagacga cggcacagtg gccgtagtcg gtcaggtcgt cgagctgcag
    21421 atggcagtcg gcccgggcag cgaccacgac gcgcaggttg ggcggcgcgt tgcgcagcag
    21481 gtaggccagc gcgtcgcggg cggacggcgg cagacggtcg gcctcgtcga tgaacaggac
    21541 gatgtccagg gcgctctgcg ccacctcggc cacccatgac gtcacgcctt cgaggccgcc
    21601 cggggccgct ccctcgaaca gcgtgtgccc gaaggtgggt cggccggcgg ccgtgcgcac
    21661 ggcgagcgtc accccgagca cgagccggtg cgcatcgtcg tgggtctgcg ccgtcagcca
    21721 ggcgacgacc gcaccgcgtg cgaggtgctc tcgccgccac tgggcgagca gcgaggtctt
    21781 gccgaagccg gcgggcgcct gcacgaggac gatgggctga tcaaacggtt cgccgtcggc
    21841 cttcaggcgc ggacgtgcga cgtgatggcg cggcacgcgc ggcgcggtga cccgtagcag
    21901 gaggtcgtcg gtgggtccga cgagagcggg cggtgcggca gcggccacgt atcgggcgag
    21961 gctcggaggg gttggacgaa gtgcggtgcg tcgatggccc acggcacggc aaatgatagt
    22021 gcgtgggttc gcgtcggacc gcccggcagc gctggctgcc gggttttgcc ggagatcgga
    22081 acccccgaaa tcccaacgca ccaggccccg atcacgtcca ggtcatgagg cggccgggcg
    22141 cctcgcttcg tcaaaccgcg ctggtgagtg cgggcaccgc gtcgaacagg tccgcttcca
    22201 gtccgtaatc cgccaccgag aagatgggcg cttcggcgtc cttgttgatc gccacgatca
    22261 ccttggagtc cttcatgccc gccaggtgct ggatcgc
//
LOCUS       DAOOCX010000033        21194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2170_length_21194_cov_7.875822, whole genome shotgun sequence.
ACCESSION   DAOOCX010000033 DAOOCX010000000
VERSION     DAOOCX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 21194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21194
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2170_length_21194_cov_7.875822"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PK399_04910"
     CDS             <1..477
                     /locus_tag="PK399_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW12495.1"
                     /translation="SALPLVTRHPNLVVTRTFSKAYALAGLRVGFAVADEGLIAVMER
                     VRESFNVNALGLVAAEAALGDVDHLNASIAGNSAQREALADALRARGMKVAPSQTNFL
                     LVEFGDHSSRIEARLVGRGVVLRPMGGYGLGECLRITVGTELENQRLLAALDEVMK"
     gene            474..1793
                     /gene="aroA"
                     /locus_tag="PK399_04915"
     CDS             474..1793
                     /gene="aroA"
                     /locus_tag="PK399_04915"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPW12496.1"
                     /translation="MSGGSDALDWIASAGSPLRGTLDIPGDKSVSHRSVMFAALADGV
                     STIEGFLEGEDTRATAAIFARLGVRIETPSPSRRIVHGVGIDGLRAADGVLDCGNAGT
                     GMRLLAGLLAAQRFDSVLVGDASLSKRPMRRVTAPLAKMGARIDTEAGGLPPLRIHGG
                     QALVGIDHTLEVASAQVKSALLLAGLYADGETIVREPHPTRDYTERMLSAFGVDIEFS
                     PGFARLRGGQRLRATDIAVPADFSSAAFFLVAAGIVPGSELRLKQVGLNPRRTGLLQA
                     LRMMGADIVEDNGGVHGGEPVADLVVRYAPLRGIEVPETLVPDMIDEFPALFIAAAHA
                     SGPTVVRGAAELRVKESDRLAAMANGLRALGLQVEETPDGATIHPGTLQGGVVDSLGD
                     HRIAMAFAVGAQRALGEVRIGDVANVATSFPGFDALARGVGCSLSDA"
     gene            complement(1873..2436)
                     /locus_tag="PK399_04920"
     CDS             complement(1873..2436)
                     /locus_tag="PK399_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW12497.1"
                     /translation="MTNVQTNYDPLLKQEDPQALQPGPDTARKTILLLGLLAITGATW
                     WTLQDRNDVVDATPAIASTEPLPAPIDEAPLAPLPTTAARPIAKSPTPGPTRDASLIA
                     SSQVLPKYPASALRAGETGTVLVLASIDRNGVPIEVSLDDRSGNREFDRSALQAVRQW
                     RFQPALRDGKPVATSVRVPVEFALERG"
     gene            complement(2589..3221)
                     /locus_tag="PK399_04925"
     CDS             complement(2589..3221)
                     /locus_tag="PK399_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW12498.1"
                     /translation="MVDPAPRKPLLPPRAVKVLAIAFGAGLLLFLLLWLDQRNDTEFF
                     KGGGPETAEGNADILPAPLPADVANEDGNASGLRLPETAQGKPLPATERPRIIDEPPP
                     PPPPPAAPVAPSAPVARGSDTPVPISQPAPRYPQEALRRNAGGTVRVRVTVATDGSVD
                     RLDVAESSGNRHLDRAAMEAVRRWRFQPAVRDGQPVVAEVVVPIVFNPGG"
     gene            3312..4592
                     /gene="serS"
                     /locus_tag="PK399_04930"
     CDS             3312..4592
                     /gene="serS"
                     /locus_tag="PK399_04930"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPW12499.1"
                     /translation="MLDPALLRANPAELAERLRTTRGFELDVGHLESLETRRKQLQVR
                     TQELQNLRNTRSKQIGMLKAKGEDVSVVMAEVAAFGDELKASEIELDELRGQIEAIAL
                     GIPNLPDASVPLGGDESQNVEQKTWGTPRTFDFEVKDHVELGARNGWLDGDTAAKLSG
                     ARFTVLRGQLARMHRALAQFMLDLHTGAHGYEETNVPVIVNADAMRGTGQLPKFEEDL
                     FSTQLGEHKRYLIPTSEVPLTNIVRDEILEADRLPLRMTAHSMCFRSEAGSAGRDTRG
                     FIRQHQFEKVELVSISKPEESDAEHERMTRCAEVVLETLGLPYRKVLLCTGDMGFGAT
                     KTYDLEVWLPSQATYREISSCSNCGDFQARRMQARWRNPATGKPEPVHTLNGSGVAVG
                     RALIAVMENYQNADGSIDVPGALRPYMGGVERIA"
     gene            complement(4686..5267)
                     /locus_tag="PK399_04935"
     CDS             complement(4686..5267)
                     /locus_tag="PK399_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPW12500.1"
                     /translation="MKLLHLDSSILGQHSASRELGAAIVARWQRDVRGLEVTYRDLAA
                     HPLPHLGAGSLAQADADEAATAAAVMDEFLSADVIVIGAPMYNFGIPSQLKAWIDRIA
                     VAGKTFRYTAEGPQGLAGGKQVIVAATYGGKHPVESGRNFVEPYLRQVFAFLGITDVE
                     FVSAEGLSISPEQRVTSISAAHARIDVALPLAA"
     gene            5382..6371
                     /locus_tag="PK399_04940"
     CDS             5382..6371
                     /locus_tag="PK399_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPW12501.1"
                     /translation="MQDLNDLYYFAAVVDHGGFAAAERALGIPKSRLSRRISALETEL
                     GVRLLQRSTRRFAVTDVGTSVHRHAQSMLAEAQAAREAVDRLSAEPRGTIRVSVPVSM
                     AQQQFPKLLPDFMALYPKVRVQLIVGNRRVDVINEGVDVALRVRAKLDDDGSLVMRSF
                     GQIQELLVASPKYLDRAGRPMAPEQLAEHVTLSISEDEARQRWELHGPDGEVRRIDLQ
                     PRITGFDFPMMQAMAEGGIGITLLPETVCADAVRRGDLEVVLPDWRLPQGIAHAVFAS
                     RRGLLPAVRVFIDFLAERLPKLLEAARLECSDPACLKAKAKAKAGSKPVQKPL"
     gene            6392..6481
                     /locus_tag="PK399_04945"
     tRNA            6392..6481
                     /locus_tag="PK399_04945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6426..6428,aa:Ser,seq:tga)
     assembly_gap    6482..6581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6691..7344
                     /locus_tag="PK399_04950"
     CDS             6691..7344
                     /locus_tag="PK399_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW12502.1"
                     /translation="MSIKVFIVDDHSLVRTGFRLILGREVDIEVLGEAESGEDALPQI
                     RKLKPDVVLCDLNLPGISGLEVTERIVRGDYGSKVIVVSVLEDGPMPRRLLEAGASGY
                     LGKACDAAELLRAVRDVARGKKYLASAIAQGIALSGMSRDADSPFDLLTPREMEVALL
                     LNQGMRQEAIAKRLSLSAKTINTHKSRLFDKLGIRDSIALARLVSQYGLVDPAHAVH"
     gene            complement(7369..10509)
                     /gene="rne"
                     /locus_tag="PK399_04955"
     CDS             complement(7369..10509)
                     /gene="rne"
                     /locus_tag="PK399_04955"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: protein motif:HMM:NF008074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HPW12503.1"
                     /translation="MKRMLINATQAEELRVAIVDGQNLYDIDIEQPSKENKKSNIYKG
                     RITRLEPSLEAAFVEYGAERHGFLPMKEISRDYFQAGVDPNKGTIRELLREGQEVVVQ
                     VDKDERGNKGAALTTFISLAGRYMVLMPNSPTAGGVSRRIEGDDRAELKKAMDALAIP
                     DDMGVIIRTAGVGRDAEELQWDLDYLLSIWRAIAEAALSKPSPFLIYQESRLIVRALR
                     DYMRSDVGEILVDTPEMYAEALDFVEQVMPHNKRKLKHYTDDTPLFNRFQIESQIESA
                     YERTVRLPSGGALVIDQTEALTAIDVNSARATKGGDIEETAFNTNLEAAEEVARQLRL
                     RDLGGLVVIDFIDMSSNKHQRDVENKLQNALKYDRARVQIGRISRFGLLEMSRQRLRN
                     SLGEASQIVCPRCEGHGRMRSIESLSLSIIRVAEEHAMKDNTGQVLVQVPVEIANFLL
                     NEKRSALREIEARHDAPIVIVADEQLHTPHYEVTRIRENEMGEETAKPSYQRGTPRRL
                     ATIALSKAQLNIPPAPAVTNVKPAQPAPLREPRPMPVEPVQPAPAPVAQVAAPQPVQA
                     SGGVIGWLRGLFTGAPAAAPEPVKAESQGGRREDRGNRGRNDRDGRGSRDSRRDERGN
                     RDEGRRNAQGEPRGNKPQGEQKQAQPQQQQKPRNDRQQQPKQDKQDKQAQPQQDKPQK
                     QPQQQKQQGDKQPQQPRQDRQQAPKPVVAEADVEIVSTTSAVAVPATGAAATMAAAQD
                     EAGLATAAGIESQDEGNGPAASDDANGEAGGKRRRGRRGGRRRRRGAGETGGVADDGL
                     LDQDGGADEALGVDGGQPEFDFDDIAPAAPQQPVVTEESRKVPSPAVAAVAAIAAAPV
                     VDAVVIPVAAAEPVTQPETAVVPEAAVVATTAPAAVQADTEAVIVESATFDNAASVAT
                     PAITTREPLVDPVVDAVPPVDDTTATAAVEVEATAIEPTQVEIAMPEMEAPIAVEQPP
                     VEQVAVAPVRIEASTAEPVTETPADAPASATLASADDEAARRAEQLRGLFQTARHDAV
                     TTPQAADDATEANRGA"
     gene            10970..11941
                     /locus_tag="PK399_04960"
     CDS             10970..11941
                     /locus_tag="PK399_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017174489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPW12504.1"
                     /translation="MTDPTPQAPPTTGARTVRVAEDRDGQRIDNFLLGYLKGAPRSLV
                     YKLLRSGQVRVNGGRIKAERRLEAGDEVRIPPIKLPDPSEKGTPPQGFMDALEAAIVF
                     EDARLLALNKPSGVASHGGSGISFGAIETLRALRPRGTLELVHRLDRDTSGLLVVAKK
                     RSALSELQALLREDHGAGIDKRYLALLGGRMPDGTMSVDAPLHVGLRQGGERHVQVNF
                     RGKPSMSHFRLLERCGGQSYCEVRIETGRTHQIRVHARHIGHPVAGDDKYGDETANRK
                     LREQAGLKRLFLHAASLEFALDGGKQPYRLTAPLAPELVAVLDRLHG"
     gene            11934..12536
                     /locus_tag="PK399_04965"
     CDS             11934..12536
                     /locus_tag="PK399_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12505.1"
                     /translation="MADTAPSLRPEMTDAEVGVLRECLSKAASLVEFGSGGSTLLAVR
                     SPSLRRIWSVESDPAWILRLRGHPEVAQAEHDGRLRFIHADIGRTGDYGMPLDETTQV
                     RWPDYYQAVWADPATTAADLVLVDGRFRVACALEALARCQPHTVLLFHDFWNRTPYHP
                     VLAFTDWLGSCDSLALLRRKASIDATHFEAVRHAHRLNPQ"
     gene            complement(12593..12934)
                     /locus_tag="PK399_04970"
     CDS             complement(12593..12934)
                     /locus_tag="PK399_04970"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HPW12506.1"
                     /translation="MADLIPLARAHCLPRHGHDHRLTDASIRELLPQLPGWELTEQGH
                     ALSRTFTFPDYYRTMSFVNALAHVANAENHHPDLSVHYDRCVVRWSTHDVGGLSENDF
                     ICAAKADALAG"
     gene            13022..13648
                     /locus_tag="PK399_04975"
     CDS             13022..13648
                     /locus_tag="PK399_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfuA family Fe-S biogenesis protein"
                     /protein_id="HPW12507.1"
                     /translation="MILISEAAQAHFRKLIERESAPGLGVRLSARDPGTPRADVRLEF
                     AGTEDLSGDEWAIDCEGFTLWLDAPSVPFLEGAEIDYTTMATGGQLQIRAPKIKGVAP
                     GEAASIVERVRWTIEQDINPQLAQHRGHVSLQEVTADGVVLLRFGGGCHGCGMADVTL
                     KQGIEKTLMEKVPGVTAVRDATDHDTGHAPFIPRGAGSESGTSPLGAA"
     gene            13664..15391
                     /locus_tag="PK399_04980"
     CDS             13664..15391
                     /locus_tag="PK399_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12508.1"
                     /translation="MTTAADVIAALNQRRQRKSLRPEAPGQFPPGWQDWFDLHPRVDV
                     ATSSAAVDGLVDAAAAKPLRAIPRSPRRLNRWQAFGVLWQQNWEPDLREDRGLRIGVR
                     VLDMVLHLLLAGALLWLMYLGYLALAHKLDEEDEAVQVEFIGRGNVAEGGGALANAGA
                     DSAPAASAPARRAATPLPASGRPDAAIAQVPMPAQPPMPVEAETPTQVQAVVPPLPAE
                     AAQPLQVSESPRPPEPTAFQLPPVRERSVQVPEVQLRELQPREQVDEVATLRPQPVRT
                     LQPSERQAQLRMPELRNQPQSLDVPAPQRMQPIAARATPDVATRQVQVPALRGEVRDI
                     PTPPGGAPTPAAQPGRGTASTANAAANGAGGERGNAASAGKAEAGSGQGSKAATQGGR
                     GVTTAGTGAGPGNKPAPGGWPGAAKTDDWGASNRNVAGTGNGNARDGDGTGKPGLYNS
                     DGSLRLPDEWTQQSGINVDRSGTWLKRPGLEYRGTRFDKYWIPQGNLLEEWVRRGIKK
                     IAIPIPGTNTRLECVVSMLQLGGGCFPVNPDINAQPAVARPPPDIPFKPQLQEDNGSV
                     KPQDPAPGG"
     gene            complement(15463..15548)
                     /locus_tag="PK399_04985"
     tRNA            complement(15463..15548)
                     /locus_tag="PK399_04985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15513..15515),aa:Leu,seq:caa)
     gene            complement(15579..16184)
                     /locus_tag="PK399_04990"
     CDS             complement(15579..16184)
                     /locus_tag="PK399_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014645425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HPW12509.1"
                     /translation="MTSSCSELQFAPVEFHGRDKFIATVDAAVASGDKHAVTSALRSA
                     LCSLIRDPDVHLPDCVHEPIVDHYARRELYRSAEHGYSVVAMTWGPGQGTPLHDHSGL
                     WCVEGVWHGELEITQYELLEVIGERARFRAAGGMIAGPGSAGSLIPPHEYHTIRNTSA
                     DAVAVSLHIYQAEMACCSKFGHREGEWYARETTELATDKAA"
     gene            complement(16238..17353)
                     /locus_tag="PK399_04995"
     CDS             complement(16238..17353)
                     /locus_tag="PK399_04995"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HPW12510.1"
                     /translation="MTEFIPPGTRWFELPSPFPIKRGGALQGARIAYETWGTLDEARS
                     NAILIVTGLSPDAHAAANAGNPAPGWWEWMVGAGKPIDTDRWFVVCVNSLGSCKGSTG
                     PASINPATGETYRLDFPELSIEDGADAAVAVVHGLGIERLACVVGNSMGGMTALALLQ
                     RHPGLARSHINISGAARALPFSIAIRSLQREAIRLDPLWNNGRYSDDAYPESGMRMAR
                     KLGVITYRSALEWDGRFGRVRLDSDRRDDDDPFGLEFEVESYLEGHARRFVRSFDPNC
                     YLYLSRSMDWFDLGEGCAGTTAQGLAGIRGLERALAIGVQTDILFPLQQQEEIADGLR
                     ASGSEATFLPLPSPQGHDAFLVDSERFGPAVRDFLGG"
     gene            complement(17514..18527)
                     /locus_tag="PK399_05000"
     CDS             complement(17514..18527)
                     /locus_tag="PK399_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011258847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HPW12511.1"
                     /translation="MVMPVFVAIAALLCLCTVAVLLRPLWRDARGVALGIGMLMLAST
                     GLLYMLVGTPRALDGTAQETPKTLGEAIAQLEAELQRDPRQAEGWRLLGQAYQREGKA
                     VQARDAYAKAVALLPDEPDLLAEAAQARALAHPRRLFDAQAVDLLTRAIAADASHQRA
                     RWFLGIAQRQAGDNAAAAATWEPLLAQVDAATAASLRKEIDAARADAGLPALAAAPKP
                     SAADASNALAVQVTLDPDFAARVRLRGDATVFVIARAPDGPPMPVAVEKRSVSELPFT
                     ATLDDGDSPMPTLKLSQMKDVVLIARLSQSGIANRQDGDIESKPVRIALPSNTPIELV
                     IGQ"
     gene            complement(18521..18943)
                     /locus_tag="PK399_05005"
     CDS             complement(18521..18943)
                     /locus_tag="PK399_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HPW12512.1"
                     /translation="MLLWLATLPAFAQANQQADAAPLQFRDAAEETRFHALATELRCV
                     MCQNQSLADSNALIALELRREVLDLMRAGKSDGQVKEHLVQRYGEFVLYKPRVEGRTW
                     LLWLGPALVLLAGAVLVVVLVRRKSAQPLPRNDEPQEW"
     gene            complement(18961..19506)
                     /locus_tag="PK399_05010"
     CDS             complement(18961..19506)
                     /locus_tag="PK399_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007964622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HPW12513.1"
                     /translation="MKTSRWLPLALFVALAALLAAGVWMSRKPDRDVLPSPLIGKPAP
                     EFALPVLHDPQHTVRLADLRGQPFLLNVWGSWCVECRVEHPVLTRFAETKRLRVVGYN
                     WKDEPADALRWLEQFGNPFWLVLADVEGRAAIDWGIYGAPETFLVDGAGIIRWKHVGP
                     LTDAVIADELMPALQQVEAAR"
     gene            complement(19503..>21194)
                     /locus_tag="PK399_05015"
     CDS             complement(19503..>21194)
                     /locus_tag="PK399_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HPW12514.1"
                     /translation="AWGNHEGSMLMWCLVACIYGFAAASRAPKDGRDESWLDARAAAV
                     QGVIATLSFAYLIFASNPFARLDPAPLRGGDLNPLLQDPALAIHPPLLYMGYVGMSVV
                     YSYAIAGLWSRAEPKAWAKAARPWALAAWSFLTIGITLGAYWAYYELGWGGWWFWDPV
                     ENASFMPWLAGTALLHSQAVTEKRGSFAGWTLLLAISAFSLSLLGTFLVRSGVLTSVH
                     AFAADPSRGLFVLVFLGIVIGGSLVLYALRAPDNDANAKPFAAGSRETLLLLNNLLLT
                     TACAMVLLGTLYPLLADALELGKISVGPPYFAMMFLLLMAPLVLLLPFGPLTRWQRED
                     MAMPLRMLLPWAALALVLGVATFFLAPQGGWKTAAGIVGATWVAAGTLRFLWSRWTMP
                     GARLTREMWGMTLAHLGIAVFLIGALLDDGLAQQREVAVKPGGSVTLGQHCFVLKGVQ
                     HLDGPNYSAERGTVEVLRHCSDPVATLHPEKRSYRGGQVMSEVALDRGFTRDLYVALG
                     EPLGEGAWALRVHVKPFVRWIWAGALLMALGGLVTATDKRFRARWDDGKPQGVRA"
BASE COUNT         3294 a         7085 c         7386 g         3329 t
ORIGIN      
        1 tccgcgctgc cgctggtgac gcgccacccg aacctggtgg ttacccgtac cttcagcaag
       61 gcgtatgcgc tggcgggcct gcgggtcggt ttcgcggtgg ccgacgaggg cctgatcgcg
      121 gtgatggaac gcgtgcgcga aagcttcaac gtcaatgcac tcggcctggt cgccgcggag
      181 gcagcactgg gtgatgtcga tcatctcaac gcgtcgatcg ccggcaacag cgcacagcgc
      241 gaggcgctcg ccgatgcatt gcgcgcgcgc gggatgaagg tggcgccgtc gcagaccaat
      301 ttcctgctgg tcgaattcgg cgaccacagc tcgcgcatag aggcgcgcct ggtcgggcgc
      361 ggcgtggtgc tgcggccgat gggcggctac gggcttggcg aatgcctgcg catcacggtg
      421 ggcaccgagc tcgagaacca gcgcctgctc gccgcgctgg atgaggtgat gaagtgagcg
      481 gtggcagcga cgccctcgac tggatagcgt ctgccggtag cccgctgcgc ggcacgttgg
      541 atatccccgg cgacaagtcg gtctcgcatc gctcggtgat gttcgccgcg ctggccgatg
      601 gcgtctcgac catcgagggt ttcctggaag gcgaggacac gcgcgccacc gcggcgatct
      661 tcgcgcgact cggcgtgcgc atcgaaaccc cgtcgccgtc gcggcgaatc gtgcatggcg
      721 tcggtatcga cgggctgcgg gccgccgatg gcgtgctgga ttgcggcaat gccggcaccg
      781 gcatgcgcct gctcgccggc ctgctggcgg cgcagcgctt cgacagcgtg ctggttggcg
      841 atgcctcgtt gtccaagcga ccgatgcggc gggtgaccgc ccccttggcc aagatgggtg
      901 cgcgcatcga caccgaagcg ggcggactgc ctccattgcg gatccatggc ggccaggcgc
      961 tggtcggcat cgaccacacg ctggaggtcg ccagtgcgca ggtgaaatcc gcgctgttgc
     1021 tggcgggcct gtatgccgat ggcgagacca tcgtgcgcga gccgcacccg acccgcgact
     1081 acaccgagcg catgctgtcc gcgttcggcg tcgacatcga gttctcgccc gggttcgcgc
     1141 gcttgcgcgg cgggcaacgc ctgcgcgcga ccgacatcgc ggtgccggcg gatttttcgt
     1201 cggccgcgtt tttcctggtg gccgccggca tcgtgcccgg ctcggaattg cgcttgaagc
     1261 aggtggggct gaacccacgc cgcaccggcc tgctgcaggc gttgcggatg atgggtgccg
     1321 acatcgtcga ggacaacggc ggcgtgcatg gcggcgaacc ggttgctgac ctcgtcgtcc
     1381 gatacgcgcc gctgcgcggc atcgaggtgc cggagacatt ggtgccggac atgatcgatg
     1441 aattcccggc gttgttcatc gctgccgcgc atgccagcgg tccgaccgtg gtgcgaggcg
     1501 ccgccgaatt gcgggtcaag gaatcggatc gcctggccgc gatggccaac gggctgcgtg
     1561 ccctgggcct ccaggtcgag gaaacgccgg atggcgcgac gatccacccc ggcacgttgc
     1621 agggcggggt ggtcgacagc cttggcgacc accgcatcgc gatggcgttc gcggtgggtg
     1681 cgcagcgtgc gcttggcgag gtgcggatcg gcgacgttgc caacgtggcg acctcgttcc
     1741 cgggcttcga tgcgctggcg cgtggcgtgg gctgctcgtt gagcgacgcc tgatcggcga
     1801 cgaccgcgct ggatcgaaat gaaacggccc gcacccggcg ggccgtttcg tcaagcggat
     1861 cgcacctcgg gatcagccgc gctcaagcgc gaactcgacc gggactcgca cgctggtggc
     1921 gaccggcttg ccatcgcgca acgcgggctg gaaacgccac tggcgcacgg cctgcagggc
     1981 ggagcgatcg aactcccgat tgccgctgcg atcgtccagg ctgacctcga tcggcacccc
     2041 attgcggtcg atgctggcca gcaccagcac ggtgccggtc tcgcctgcgc gcagcgccga
     2101 tgccgggtac ttcggcagca cctggctgct ggcgatcagc gaggcatcac gcgtcgggcc
     2161 gggggtcggc gacttcgcga tcggccttgc agcggtggtt ggcagcggcg ccaggggcgc
     2221 ttcgtcgatc ggggccggca ggggttcggt cgaggcaatc gcaggcgtgg catcgaccac
     2281 gtcgttgcga tcctggaggg tccaccacgt tgcgccggtg atggcgagca ggcccagcag
     2341 caggatcgtc ttgcgcgccg tgtcgggacc tggctgcaac gcctgcgggt cctcctgttt
     2401 cagcagcgga tcgtagttgg tctggacatt cgtcatgtcg atctcctcgt cgtgcggaag
     2461 gcacgcgacc gagtgtcctg atcgactcgt tagacgggga tcaatccgcg caggattacg
     2521 tgcttgggct ccgccctgga ttcagcgatg tcgtcatcgc gtccacgttt cgttgtgcgt
     2581 gggtgacgtc agccgcccgg attgaacacg atcggaacca cgacctcggc gacgacaggc
     2641 tggccatcgc gcaccgcggg ctggaagcgc cagcggcgca ccgcttccat cgcggcgcga
     2701 tccaggtggc gattgccgct gctttccgcg acgtccaggc gatcgacgct gccgtcggtg
     2761 gccacggtga ccctcacgcg tacggtgcca ccggcgttcc ggcgcaacgc ctcctgcgga
     2821 taacgcggcg cgggctggct gatcggcacc ggggtatcgc tgccccgggc gaccggcgcg
     2881 gagggagcga cgggtgcggc aggtggcggt ggcggggggg gcggttcatc gatgatgcgg
     2941 gggcgctcag tcgcaggcag cggcttgccc tgcgcggtct cgggcaggcg caggccgctg
     3001 gcgttgccgt cctcgttggc cacatcggcc ggcaagggtg ccggcaggat gtcggcgttg
     3061 ccttcggcgg tctcgggccc gccgcccttg aagaattcgg tgtcgttgcg ctggtccagc
     3121 cacagcagca ggaacagcaa caggccagcg ccgaacgcaa tggcgaggac cttgacggcc
     3181 cggggcggca gcagcggctt gcgcggtgcg ggatcgacca tgccgcattc tggcacagcg
     3241 catcgtcgtg ccagcgccaa cgcgcgccgg ctcgggcgat aatgagcatt cgtccacgga
     3301 accccctcgc gatgctcgat cccgccctgt tgcgcgccaa ccccgccgaa ctcgccgaac
     3361 gcctgcgcac cacgcgcggc ttcgaactgg acgtcggcca cctcgagtcg ctggaaacgc
     3421 gtcgcaaaca gctgcaggtg cgcacccagg aactgcagaa cctgcgcaat acccgcagca
     3481 agcagatcgg catgctcaag gcaaagggcg aggacgtctc cgtggtgatg gccgaagtcg
     3541 ccgcgttcgg cgatgaactg aaggcctcgg aaatcgaact cgacgagttg cgtggccaga
     3601 tcgaggccat cgcgctgggc atcccgaacc tgccggatgc ttcggtgccg cttggcggcg
     3661 acgagagcca gaatgtcgag cagaagacct ggggcacgcc caggaccttc gatttcgaag
     3721 tgaaggacca cgtcgagctc ggcgcgcgca acggctggct ggatggcgat accgccgcca
     3781 agctgagcgg cgcgcgcttc accgtgctgc gtggccagct ggcacggatg catcgcgcac
     3841 tcgcccagtt catgctcgac ctgcacaccg gcgcgcacgg ttacgaggaa accaacgtgc
     3901 cggtcatcgt caacgccgat gcgatgcgcg gcaccggcca gttgccgaag ttcgaggaag
     3961 acctgttctc cacccagctc ggcgaacaca agcgctacct gatccccacg tcggaagtgc
     4021 cgctgaccaa catcgtccgg gacgagatcc tggaagcgga tcgcctgcca ctgcggatga
     4081 ccgcgcactc gatgtgcttc cgctccgagg ccggcagcgc cggccgcgac acccgcggtt
     4141 tcatccgcca gcatcagttc gagaaggtgg agctggtcag catcagcaaa cccgaagagt
     4201 cggatgccga gcacgagcgc atgacccgct gcgccgaagt ggtgctggaa acactcggcc
     4261 tgccgtatcg caaggtgctg ctgtgcaccg gcgacatggg cttcggcgcg accaagacct
     4321 acgacctgga agtctggctg ccatcgcagg ccacgtatcg cgagatttcg tcctgttcca
     4381 actgcggcga tttccaggcg cggcgcatgc aggcgcgctg gcgcaacccg gccaccggca
     4441 aacccgaacc ggtgcatacc ttgaacggtt ccggcgtggc cgtgggccgc gcgttgatcg
     4501 cggtgatgga gaattaccag aacgccgatg gcagcatcga cgtgccgggg gcgctacgtc
     4561 cgtacatggg cggcgtcgaa cgcatcgcct gatcgttccc gcaatctcga aaaaaaaaac
     4621 gggcccgcag tgcgggcccg ttgcgttccg gggaggggag ggaggaacgc gaaagcggtg
     4681 ttcgatcagg ctgcaagcgg cagcgcgaca tcgatgcggg catgtgcggc cgagatcgac
     4741 gtcacccgct gctccggcga gatggacaac ccctcggcgc tgacgaattc gacatcggtg
     4801 atgccgagga aggcgaacac ctggcgcagg taaggttcga cgaagttgcg cccgctttcg
     4861 accgggtgct tgccgccgta ggtcgcggcc acgatcacct gcttgccgcc ggccaggccc
     4921 tgcgggccct cggcggtgta gcggaaggtc ttgccggcca ccgcgatgcg atcgatccag
     4981 gccttcaact ggctgggaat gccgaagttg tacatcggcg cgccgatcac gatcacgtcg
     5041 gcggacagga attcgtccat caccgccgcc gcggttgcgg cctcgtcggc atcggcctgt
     5101 gcgagcgaac cagcgcccag gtgcggcagc ggatgggcgg cgaggtcgcg gtaggtgacc
     5161 tcgaggccac gcacgtcgcg ctgccagcgg gcgacgatcg cggcgcccag ttcgcgactg
     5221 gcggaatgtt ggccaaggat gctggagtcg agatgcagga gtttcatgct gatgccccgt
     5281 cgggtgcggc agcgccgcgt tgggatgcat cctcctctgt tgcatattcg ggataaagac
     5341 tgttcaatat catgatacgt tccatattcg gaattatcgt catgcaggat ctcaacgatc
     5401 tctattactt cgccgcggtc gtggaccacg gtggattcgc cgccgcggaa cgcgcgctcg
     5461 gcatccccaa gtcgcggctg agccggcgca tcagtgcgct ggagaccgag cttggcgtgc
     5521 gcctgttgca gcgctcgacg cgacggttcg cggtgacgga cgtggggacc agcgtgcatc
     5581 gccatgcgca gtcgatgctg gccgaggcgc aggcggcgcg cgaagccgtg gatcgcctga
     5641 gcgcggagcc gcgcggcacg atccgggtca gcgtaccggt gtcgatggcg cagcagcagt
     5701 tcccgaagct gctgccggac ttcatggcgc tgtacccgaa ggtgcgcgtg cagttgatcg
     5761 tcggcaatcg ccgcgtcgac gtgatcaacg agggtgtcga tgtcgccctg cgcgtgcgcg
     5821 cgaaactcga tgacgacggc agcctggtga tgcgcagctt cggccagatc caggagttgc
     5881 tggtcgccag cccgaagtac ctggaccgcg ccggccgtcc gatggcgccg gagcaactcg
     5941 ccgagcacgt caccctcagc atcagcgagg acgaagcccg ccagcgttgg gaactgcacg
     6001 gccccgatgg cgaagtgcgc cgcatcgacc tgcaaccgcg gatcaccggc ttcgatttcc
     6061 cgatgatgca ggcgatggcc gagggcggta tcggcattac gctgctgccg gaaaccgtgt
     6121 gcgcggatgc ggtgcggcgc ggcgacctgg aagtggtgct gccggattgg cgcctgccgc
     6181 agggcatcgc ccatgccgtg ttcgcctccc gccgcggcct gctgccggcg gtgcgggtgt
     6241 tcatcgactt cctggccgag cgactgccga aactgctgga ggcagcacgc ctcgagtgca
     6301 gcgaccccgc ctgcctcaag gcaaaggcaa aggccaaggc aggttcgaag ccggtccaga
     6361 agccgctctg acgtgcgaaa atccgcatca cggaagtgtg gccgagtggt ttaaggcagc
     6421 ggtcttgaaa accgccgatg ggtaaaacca tccgtgagtt cgaatctcac cgcttccgcc
     6481 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngacgcattc gcataggggt
     6601 aggacttttc ctactcgcca gcgcggcata cgctgctggg atgggacggg tgggttcggt
     6661 agcgtgtccc catcgaatcg gggggctgcc atgagcatca aggttttcat cgtcgacgac
     6721 cattcgctgg tgcgcacggg cttccgcctg atcctgggcc gtgaagtcga catcgaagtg
     6781 ctcggcgagg ccgaaagcgg cgaggacgca ctgccgcaaa tccgcaagct caagcccgac
     6841 gtggtcctct gcgacctcaa cctgccgggc atcagcgggc tggaggtgac cgagcgcatc
     6901 gtgcgcggcg actacggcag caaggtgatc gtggtgtcgg tgctggagga cggaccgatg
     6961 ccgcgccgct tgctggaggc cggtgcgtcc ggttacctcg gcaaggcctg cgatgcggcc
     7021 gagttgttgc gtgcggttcg cgacgtggca cgaggtaaga aatacctggc cagcgccatc
     7081 gcccagggca tcgcgttgtc ggggatgagt cgcgatgccg actcgccctt cgacctgctg
     7141 accccgcgcg agatggaggt cgccctgctg ctcaaccagg gcatgcgcca ggaagccatt
     7201 gccaagcgcc tcagcctgag cgccaagacg atcaacaccc acaagtcccg gctcttcgac
     7261 aagctcggca tccgcgacag catcgcgctg gcgcggttgg tcagccagta cggcttggtc
     7321 gatcccgcgc acgccgtcca ctgacggcgc gcgcagggcc aagcggcgtc aggcgccgcg
     7381 gttggcctcg gtggcgtcgt ccgccgcctg cggagtggtc acggcgtcgt ggcgagccgt
     7441 ctggaacagg ccacgcaatt gctcggcgcg gcgcgccgcc tcatcgtctg ccgaagcgag
     7501 ggtcgcggac gcgggtgcat cggccggggt ttcggtcacc ggctccgcgg tcgaagcctc
     7561 gatacgcacc ggcgcgacgg cgacctgctc gacagggggc tgctcgacag cgatgggcgc
     7621 ttccatctcc ggcatcgcga tctcgacctg cgtcggttcg atcgcagtcg cctcgacttc
     7681 caccgccgcg gtcgcggtgg tgtcgtcgac cggcggaacg gcgtcgacca ccgggtccac
     7741 gaggggctcg cgcgtcgtga tggccggggt tgccacgctg gcggcgttgt cgaaggtggc
     7801 gctctcgacg atcaccgctt ccgtgtccgc ctgcaccgct gcgggcgccg tggtggcaac
     7861 cacggcagct tcgggcacga ctgctgtttc gggctgggta accggctcgg cggcagcaac
     7921 gggaatgacg actgcgtcga cgaccggtgc ggcagcgatc gcggccaccg cggcgacggc
     7981 gggcgagggc accttgcggc tttcttccgt cacgacgggt tgctgcggcg cggccggcgc
     8041 gatgtcgtcg aagtcgaact cgggctggcc gccatcgacg cccaaggcct cgtcggcgcc
     8101 accgtcctgg tccagcaagc cgtcatcggc cacgccaccg gtttcgccgg cgccacgacg
     8161 acgacgacga ccaccgcggc ggccgcggcg gcgcttgccg ccggcttcgc cattggcgtc
     8221 atcggatgcg gccggtccat tgccttcgtc ctgcgattcg atgccggcgg ccgtggccag
     8281 accggcttcg tcctgcgctg ccgccatcgt tgccgccgca ccggtcgccg ggacagcgac
     8341 cgccgaggtg gtcgacacga tctcgacatc ggcctcggcg accacgggct tcggagcctg
     8401 ttgcctgtcc tgccggggct gctgcggctg cttgtcgccc tgctgcttct gctgctgggg
     8461 ctgcttttgc ggcttgtcct gctgcggctg ggcctgcttg tcctgcttgt cctgcttggg
     8521 ctgctgttgc ctgtcgttgc gcggcttctg ttgctgctgc ggctgggcct gcttctgctc
     8581 gccctgcggc ttgttgccgc gcggctcgcc ctgcgcattg cgacggcctt cgtcgcgatt
     8641 gccacgctcg tcgcgacgcg aatcgcggct gccacggcca tcgcggtcgt tgcggccgcg
     8701 gttgccgcga tcttcgcgac ggccgccctg cgactcggcc ttgaccggtt ccggcgccgc
     8761 ggcgggtgcc ccggtgaaca ggccgcgcaa ccagccgatc acgccgccac tggcctggac
     8821 cggctgcggc gcggccacct gggcgaccgg cgcgggcgcc ggctgcacgg gttccaccgg
     8881 catcggccgc ggctcgcgca gcggtgccgg ctgggcaggc ttgacgttgg tcacggccgg
     8941 tgccggcggg atgttcagct gtgccttgct cagggcgatg gtggcgagcc tgcgcggggt
     9001 gccgcgctgg taactgggct tggcggtttc ctcgcccatt tcgttttcgc ggatgcgcgt
     9061 gacttcgtag tgcggggtgt gcaattgctc gtcggcgacg atcacgatcg gcgcgtcgtg
     9121 gcgtgcctcg atctcgcgca gggcgctgcg cttctcgttg agcaggaagt tggcgatttc
     9181 caccggcacc tgcaccagta cctggccggt gttgtccttc atcgcatgct cttcggcgac
     9241 gcggatgatc gacagcgaca gcgactcgat gctgcgcatg cggccatggc cctcgcagcg
     9301 cgggcagacg atctggctgg cttcgcccaa gctgttgcgc aggcgctggc ggctcatctc
     9361 gagcaggccg aagcggctga tgcggccgat ctgcacgcgt gcgcggtcgt acttgagcgc
     9421 gttctgcagc ttgttctcga cgtcgcgctg gtgcttgttg gacgacatgt cgatgaagtc
     9481 gatcaccacc aggccgccca ggtcgcgcag gcgcagctgg cgcgccactt cctcggccgc
     9541 ctccaggttg gtgttgaacg cggtttcctc gatgtcgccg cccttggtgg cgcgcgccga
     9601 gttcacgtcg atcgcggtca gggcctcggt ctggtcgatc accagcgcgc cgccggaggg
     9661 caggcgcacg gtgcgctcgt aagccgactc gatctgcgac tcgatctgga agcggttgaa
     9721 cagcggagtg tcgtcggtgt agtgcttgag cttgcgcttg ttgtgcggca tcacctgttc
     9781 gacgaagtcc agcgcctcgg cgtacatctc cggggtgtcg accaggatct cgcccacgtc
     9841 ggagcgcatg tagtcgcgca gggcgcgcac gatcaggcgc gactcctggt agatcaggaa
     9901 cggcgacggt ttgctcagcg cggcctcggc gatcgcccgc cagatgctca gcaggtagtc
     9961 gaggtcccac tgcagttctt cggcatcgcg acccacgccg gcggtgcgga tgatcacgcc
    10021 catgtcgtcg gggatcgcca gcgcgtccat cgccttcttc agctcggcgc ggtcgtcgcc
    10081 ctcgatccgg cgcgagacgc cgccggcggt cggcgaattc ggcatcagca ccatgtagcg
    10141 gccggccagc gagatgaacg tggtcagggc agcgcccttg ttgccgcgct cgtccttgtc
    10201 gacctggacc acgacttcct ggccctcgcg cagcagctcg cggatcgtgc ccttgttcgg
    10261 gtcgacgccg gcctggaagt agtcgcggga gatttccttc atcggcagga agccgtggcg
    10321 ttccgcgccg tattcgacga aggccgcctc gagggacggc tcaagccggg tgatccggcc
    10381 cttgtagatg ttggacttct tgttttcctt ggacggctgt tcgatgtcga tgtcgtacag
    10441 gttctggccg tccacgatgg ctacccgcag ttcttccgcc tgcgtggcat tgatcagcat
    10501 tcgcttcatg gtgggttcct agcgcgcgtc tgcgcgcgga acgccgcggg cgtttcacct
    10561 ctggataccg caagcgccgc ggctgcgtga gtgcgcgccg cgtcgctcca agtgttccag
    10621 cgctgcaaca ccacggcatg ccgcgggagc gcttgtttcc tggttcgtgt tgcagggccg
    10681 gcgacagccg ccggagggcg ggtcgcgcgg gacattgtgt tcctggtccg cacgggcgca
    10741 tgcgcccggc aatggcgggg cttgccgcgc agccgttaac atggccgtcc cggggacggt
    10801 ggcaacgaca ctgcagcggg ctcgcgggga aggccagctt tcggccggcg gcttgtggcc
    10861 gggtccactt ccctgcgaaa tcaaaccctt acatcgcccc ccgagtgtat cagatcaagc
    10921 gcgacagcag caggcccaag acgggcaggc ccacaggagc atgcccccga tgaccgaccc
    10981 gacgccccag gccccgccga ccaccggcgc gcgcaccgtg cgcgtggcgg aagaccgcga
    11041 cggccagcgc atcgacaatt tcctgttggg ctacctgaag ggagcgccgc gcagcctggt
    11101 ctacaagctg ttgcgctccg gccaggtgcg ggtgaacggc gggcggatca aggccgagcg
    11161 gcgcctggaa gcgggtgacg aggtgcggat cccgccgatc aagctgcccg accccagcga
    11221 gaagggcacg ccgccgcagg gcttcatgga cgcgctggag gccgcgatcg tgttcgagga
    11281 cgcccgcctg ctggccctga acaagccgtc cggtgtcgcc agccacggtg gcagcgggat
    11341 ttccttcggt gccatcgaga ccctgcgggc gctgcggccg cggggaacgc tcgagctggt
    11401 ccaccggctg gatcgcgaca cgtccggcct gctggtcgtg gccaagaagc gcagtgccct
    11461 cagcgaactg caggccctgt tgcgtgaaga ccatggcgcc ggcatcgaca agcgttacct
    11521 ggccctgctg ggcggtcgga tgccggacgg gacgatgtcg gtggatgcgc ccctgcatgt
    11581 cggcctgcgc cagggcggcg agcgccacgt gcaggtgaat ttccgcggca agccgtcgat
    11641 gagccatttt cgcctgttgg agcgttgcgg cggacaaagc tattgcgagg tgcgcatcga
    11701 gaccggccgc acccaccaga tccgcgtgca tgccaggcac atcggccatc cggtggctgg
    11761 cgacgacaag tacggcgacg aaaccgccaa ccgcaagctg cgcgagcagg ccggactgaa
    11821 gcgcctgttc ctgcatgccg ccagcctgga attcgcgctg gacggcggca agcagccata
    11881 tcggctgacc gcgccgctgg caccggagct ggtggcggtg ctggatcgcc tgcatggctg
    11941 acaccgcacc gtcgctgcgg ccggagatga ccgatgcgga ggtcggcgtg ctccgcgagt
    12001 gcctgtccaa ggcggccagc ctggtcgagt tcggcagcgg cggcagcacc ctgttggccg
    12061 tgcgtagccc gtcgctgcgg cggatctggt cggtggagag cgacccggcc tggatcctca
    12121 ggctgcgcgg acaccccgag gtggcgcagg ccgaacatga cgggcgcctg cgcttcatcc
    12181 atgccgacat cggccgcacc ggcgactacg ggatgccgct ggacgaaacc acgcaggtgc
    12241 gctggcccga ctactaccag gcggtctggg cagacccggc gaccaccgcg gccgacctgg
    12301 tcctggtcga tggccgtttc cgcgtcgcct gtgcgctgga agcgctggcg cgatgccagc
    12361 cgcacaccgt cctgctgttc cacgatttct ggaaccgcac cccctaccac ccggtgctgg
    12421 cgttcaccga ctggttgggc agctgcgatt ccctggcgct cctgcggcgc aaggcgagca
    12481 tcgatgccac gcacttcgag gcggtgcggc atgcgcaccg gctgaatccg caatgagagg
    12541 cccgtcggtc agcggacgct gagccacgac ggcaacgact gtcggcaggg actcagccgg
    12601 cgagggcgtc ggccttggcg gcacagatga agtcgttctc ggacaggcca cccacatcgt
    12661 gggtggacca gcgcaccacg cagcggtcgt agtgcaccga gagatcggga tgatggttct
    12721 cggcattggc cacgtgcgca agcgcgttga cgaaggacat cgtccggtaa tagtcgggga
    12781 aggtgaaggt gcggctcagc gcgtggccct gttcggtcag ttcccagccg ggcagctgcg
    12841 gcagcagttc gcggatgctg gcgtcggtca gccggtggtc gtggccgtgg cgtggcaggc
    12901 agtgcgcccg tgcgagcggg atcaggtcgg ccatggcgat gtcctttggg aatgaaccgg
    12961 gaatggcggc acgccggtgc gcgagggatg cgcatcgcgg ctgcatcgtt agaataccgg
    13021 catgatcctg atttccgaag ccgcgcaggc gcacttccgc aagctcatcg aacgcgaatc
    13081 cgcgcctggc ctgggggtgc gcctgtccgc gcgcgacccg ggtacgccgc gcgcggacgt
    13141 gcggctcgag ttcgccggca ctgaagacct cagcggcgac gaatgggcga tcgattgcga
    13201 gggcttcacc ctctggctgg atgcgcccag cgtgccgttc ctggagggtg ccgagatcga
    13261 ctacaccacc atggccaccg gcggccagct gcagatccgc gccccgaaga tcaagggcgt
    13321 ggcaccgggc gaagcggcgt cgatcgtcga gcgcgtgcgc tggaccatcg agcaggacat
    13381 caatccgcaa ctggcccagc accgcggcca tgtgagcctg caggaagtca ccgcggacgg
    13441 cgtggtgctg ttgcgcttcg gcggcggttg ccacggctgc ggcatggccg acgtgaccct
    13501 gaagcagggc atcgagaaga ccctgatgga gaaggtgccg ggcgtgaccg ccgtgcgcga
    13561 cgccaccgac cacgataccg gccacgcgcc gttcatcccg cgcggcgcgg gatccgaatc
    13621 cggcacgtcg ccgctcggcg cggcctgacc ggatcggccg cgcgtgacga cagccgctga
    13681 cgtcatcgcc gcgctcaacc agcgccgcca acgcaaatcg ttgcgcccgg aagcacccgg
    13741 gcagttcccg cccggttggc aggactggtt cgacctgcat ccgcgggtgg acgtggcgac
    13801 gtccagcgcg gcggtcgacg gcctggtcga tgccgctgcg gccaagccgt tgcgtgccat
    13861 cccgcgcagc ccgcgccgct tgaaccgctg gcaggcgttc ggcgtgctgt ggcagcagaa
    13921 ctgggaaccc gacctgcgcg aggaccgcgg cctgcgcatc ggcgtgcgcg tgctggacat
    13981 ggtcctgcac ctgctgctgg ccggcgcgct gttgtggctg atgtacctgg gctacctggc
    14041 gctggcgcac aagctggacg aggaagacga agcggtgcag gtcgagttca tcggccgcgg
    14101 caacgtcgcc gaagggggcg gtgcactggc gaatgccggt gcggattccg ccccggccgc
    14161 atccgccccg gcccgacgcg cggccacgcc gttgcccgcg agcggtcgcc ccgatgccgc
    14221 gatcgcccag gtgccgatgc ccgcgcagcc gccgatgccg gtcgaggcgg aaacaccgac
    14281 ccaggtgcaa gccgtggtcc cgccactgcc ggcggaggcg gcacaaccct tgcaggtcag
    14341 cgagtcgccc aggccgcccg agccaacggc gttccagttg ccgcccgtgc gcgagcgcag
    14401 cgtgcaggtg cccgaggtgc agttgcgcga gctgcagcca cgcgagcagg tggacgaggt
    14461 ggcgaccctg cggccgcaac ccgtgcgtac cctgcagccg agcgaacgcc aggcgcagtt
    14521 gcgcatgccc gaattgcgca accagccgca atcgctggat gtccccgccc cgcaacgcat
    14581 gcagcccatt gcggcgcggg cgacacccga tgtcgccacc cgccaggtgc aggtgccggc
    14641 cctgcgtggc gaagtccgcg acattccgac gccgcccggc ggcgcaccga cgcccgcggc
    14701 ccagcccggg cgcggcacgg catccactgc caatgccgct gccaacgggg ccggtggaga
    14761 acgtggcaac gccgcctccg ccggcaaggc ggaggcaggc agcgggcagg gcagcaaggc
    14821 ggcgacgcag ggcggacgcg gcgtaacgac tgccggaacg ggcgcgggtc ccggcaacaa
    14881 accggcaccc ggtggctggc cgggcgcggc caagaccgac gactggggtg cgtccaaccg
    14941 caacgtcgcg gggacgggca acggcaatgc ccgggatggc gacggcaccg gcaagcccgg
    15001 tctctacaac agcgacggca gcctgcgctt gccggacgag tggacgcagc agagcggcat
    15061 caacgtcgat cgctccggca cgtggctgaa gcgcccgggg ctggaatacc gcggcacccg
    15121 gttcgacaag tactggatcc cgcagggcaa tctgctggag gaatgggtgc ggcgcggcat
    15181 caagaagatc gccattccga tcccgggcac caatactcga ctggaatgcg tggtgtccat
    15241 gctgcaactc ggcggcggtt gcttcccggt gaatcccgac atcaacgcgc aacccgccgt
    15301 tgctcgcccg ccgccggaca ttccgttcaa gccgcagctc caggaagaca acggcagcgt
    15361 gaagccgcag gacccggccc ccggcggttg accgcacgct tggttcacga cccggccgac
    15421 gcctgaaaac agaaaagcca cgcattgcgt ggcttttcat cttggtaccg gtgagaggac
    15481 tcgaacctcc acgacatcgc tgccggcgga ttttgaatcc gctgcgtcta ccattccgcc
    15541 acaccggcac gggcgcgaag tatagcccag tgcgtggctc aggcggcctt gtcggtggcg
    15601 agttcggtgg tctcgcgcgc gtaccactcg ccttcgcgat ggccgaactt ggaacagcag
    15661 gccatctccg cctggtagat atgcagcgat accgccaccg cgtccgcgct ggtgttgcgg
    15721 atggtgtggt actcgtgcgg tgggatcagg ctgccggcgc tgcccgggcc cgcgatcatg
    15781 ccgcccgcgg cacggaagcg cgcgcgctcg ccgatgacct cgagcaattc gtactgggtg
    15841 atttccagct cgccgtgcca cacgccttcc acgcaccaca gtccggagtg gtcgtgcagc
    15901 ggcgtgccct ggcccggtcc ccaggtcatc gcgaccacgc tgtagccatg ttcggcgctg
    15961 cgatacagct cgcgtcgcgc gtaatggtcg acgatcggct catgcacgca gtcgggcagg
    16021 tggacgtcgg ggtcgcggat caggctgcac agggcgctgc gcagcgcgga ggtcaccgca
    16081 tgcttgtcgc cggacgcgac cgcggcatcg accgtcgcga tgaacttgtc gcggccgtgg
    16141 aattcgaccg gagcgaattg gagttcggaa caggacgagg tcatggccga agtctactaa
    16201 aggttatgtc gtcatgcccg ggtggcatga ccggcggtca gccgcccagg aagtcgcgga
    16261 cggccggacc gaagcgctcg ctgtcgacca ggaaggcgtc atggccctgc ggcgagggca
    16321 gggggaggaa cgtggcctcg ctgccgctgg cgcgcaggcc atcggcaatc tcttcctgct
    16381 gctgcagcgg gaacaggatg tcggtctgca cgccgatcgc cagcgcgcgt tccagcccgc
    16441 ggatcccggc cagtccctgc gccgtggtgc cggcgcagcc ttcgcccagg tcgaaccagt
    16501 ccatcgagcg gctcaggtag aggtagcagt tcgggtcgaa gctgcgcacg aacctgcgcg
    16561 catgaccttc caggtagctt tcgacctcga actccaggcc gaacgggtca tcgtcgtcgc
    16621 ggcgatcgga gtccagccgc accctgccga aacggccgtc ccactccagt gcggagcgat
    16681 aggtgatcac ccccagcttg cgcgccatgc gcatgccgga ctcgggatag gcatcgtcgc
    16741 tgtatcggcc gttgttccac agcggatcca ggcggattgc ctcacgctgc agcgagcgga
    16801 tcgcgatcga gaacggcagc gcacgcgccg cgcccgagat gttgatgtgc gagcgggcga
    16861 ggccgggatg ccgctgcagc agcgccagcg cggtcatccc gcccatcgaa ttgccgacca
    16921 cgcaggccag ccgctcgatc cccaatccgt gcaccacggc gaccgcggcg tcggcgccgt
    16981 cctcgatcga cagttccggg aaatcgaggc ggtaggtctc gccggtagcc gggttgatcg
    17041 aggccggccc ggtcgagccc ttgcaactgc caagcgaatt cacgcagacc acgaaccagc
    17101 ggtccgtgtc gatcggcttg cccgcgccca ccatccattc ccaccagccc ggcgcgggat
    17161 tgcccgcgtt ggccgctgca tgcgcgtccg gcgacagccc ggtgacgatc aggatcgcat
    17221 tgctgcgtgc ctcgtcgagc gtgccccagg tttcataggc gatgcgtgca ccctgcaagg
    17281 cacccccgcg cttgatcggg aacggcgagg gcagctcgaa ccagcgggtg ccgggaggaa
    17341 tgaattcggt catgggtgaa gtttagctgg cgcttgtctc cctctcccgc ttgcgggaga
    17401 gggttggggt gagggcggcg cctttcgcgc acctctccac cctcatctgc cctgcgggca
    17461 tcctcagccg cccagccgcg gcttcgatcc cgccagcggg cgaagaaaat cggctattgc
    17521 ccgatcacca attcgatcgg cgtgttgctg ggcaacgcga tccgcaccgg cttcgattcg
    17581 atgtcgccgt cctgcctgtt ggcgatgccg ctttgcgaca gccgcgcgat cagcacgacg
    17641 tccttcatct gcgacagttt gagcgtgggc atcgggctgt cgccatcgtc gagggtggct
    17701 gtgaacggca gctcggacac gctgcgtttc tccaccgcga ccggcatcgg cgggccgtcc
    17761 ggcgcacgcg cgatcacgaa cacggtggca tcgccgcgca ggcgcacgcg tgccgcgaag
    17821 tcgggatcca gcgtgacctg gacggcgagt gcgttgcttg cgtcggctgc cgatggcttg
    17881 ggcgcagccg cgagcgcagg caggcctgca tcggcacggg cggcatcgat ttccttgcgc
    17941 aggcttgcgg cggtcgcggc atcgacctgc gccagcagcg gttcccaggt ggcggcggcc
    18001 gcggcgttgt cgccggcctg ccgctgggcg atccccagga accagcgcgc gcgctggtgg
    18061 ctggcgtcgg ctgcgatggc acgcgtgagc aggtcgaccg cctgcgcatc gaacaatcgc
    18121 cggggatgcg ccagcgcacg cgcttgcgcg gcttcggcca gcaggtccgg ttcgtcgggc
    18181 agcaacgcca cggcctttgc atacgcatca cgcgcctgca cggccttgcc ttcgcgctgg
    18241 taggcctggc ccagcagccg ccagccttcg gcctggcgcg gatctcgttg cagttcggcc
    18301 tcgagctggg cgatcgcctc gcccagggtc ttcggggttt cctgcgccgt accatccagc
    18361 gcgcgcggcg tgcccaccag catgtacaaa aggccggtcg aggccagcat cagcatgccg
    18421 atgcccaggg cgacgccgcg tgcgtcgcgc cacaacgggc gcagcagcac ggcgacggtg
    18481 cacaggcaga gcagggcggc gatcgcgacg aagacgggca tcaccattcc tgcggttcgt
    18541 cgttacgggg cagcggttgc gcggacttgc ggcggaccag caccacgacc agcaccgcgc
    18601 ccgccaacag gaccagggcc ggccccagcc acaacagcca ggtgcggccc tcgacgcgcg
    18661 gcttgtacag cacgaactcg ccgtagcgct gcaccaggtg ctccttgacc tgcccgtcgc
    18721 tcttgcccgc acgcatcagg tccagcacct cgcggcgcag ctccagtgcg atcagtgcat
    18781 tggaatcggc cagcgactgg ttctggcaca tcacgcagcg cagttcggtg gccaacgcgt
    18841 ggaagcgcgt ttcctcggcg gcgtcgcgga attgcagcgg cgcggcatcg gcttgctggt
    18901 tggcctgcgc gaatgcgggc agcgtggcca accacagcag catggcgagg atcaaccgca
    18961 tcagcgcgca gcctcgacct gttgcagcgc cggcatcaac tcatcggcga tcacggcgtc
    19021 ggtgagcggg cccacgtgct tccagcggat gatgccggcg ccatcgacca ggaaggtttc
    19081 cggcgcgccg tagatgcccc agtcgatcgc cgcgcggcct tccacatcgg ccagcaccaa
    19141 ccagaacggg ttgccgaact gctccagcca gcgcagcgcg tcggcgggtt cgtccttcca
    19201 gttgtagccg acgacgcgca ggcgcttggt ctcggcgaag cgggtcagca ccgggtgttc
    19261 gacccggcat tccacgcacc agctgcccca gacgttgagc aggaacggtt ggccgcgcag
    19321 gtcggccagc cgcaccgtgt gttgcgggtc gtgcagcacc ggcaaggcga actcgggtgc
    19381 cggtttgccg atcagcggcg agggcaacac gtcacgatcc ggcttgcggc tcatccacac
    19441 gccggcggcc agcagcgcgg ccagcgcgac gaagagggcg agcggcagcc agcgcgaggt
    19501 tttcatgcac ggactccctg cggtttgccg tcatcccagc gcgccctgaa acgcttgtcg
    19561 gtcgcggtca ccaatccgcc gagtgccatc agcagcgctc cggcccagat ccagcgcacg
    19621 aacggcttca cgtgcacgcg cagggcccag gcaccttcgc ccaggggttc gcccagcgcg
    19681 acatagagat cgcgggtgaa tccgcggtcg agggcgactt cgctcatcac ctggccgccg
    19741 cgatagctgc gcttttccgg gtgcagggtg gcgacggggt ccgagcaatg ccgcagcacc
    19801 tcgacggtcc cgcgctcagc gctgtagttc ggcccgtcca ggtgctgcac gcccttcagg
    19861 acgaagcagt gctggcccaa cgtcaccgag ccgcccggct tcaccgcgac ctcgcgctgc
    19921 tgcgccaggc cgtcatccag cagcgcgccg atcaggaaca ccgcgatgcc caagtgcgcc
    19981 agggtcatgc cccacatttc ccgggtcagg cgtgcacccg gcatggtcca gcgcgaccag
    20041 aggaaacgca gggtgccggc cgcgacccaa gtcgcgccaa cgatgcccgc cgcggtcttc
    20101 cagccaccct gcggggccag gaagaacgtg gccacgccca gcaccagcgc cagtgccgcc
    20161 catggcaaca gcatgcgcag cggcatcgcc atgtcctcgc gctgccagcg ggtcagcgga
    20221 ccgaacggca gcagcaatac cagcggcgcc atcagcagca ggaacatcat cgcgaagtag
    20281 ggcgggccga ccgagatctt gccgagctcc agcgcatcgg ccagcagcgg atacagcgtg
    20341 ccgagcagga ccatcgcgca ggcggtggtc agcagcaggt tgttgagcag cagcagggtc
    20401 tcgcgcgaac cggcggcgaa tggcttggcg ttggcgtcgt tgtccggtgc gcgcaacgca
    20461 tacagcacga gcgagccgcc gatgacgatg cccaggaaaa ccagcacgaa cagcccgcgc
    20521 gacggatccg cggcgaacgc atgcacgctg gtgagtacgc cggaacggac caggaaggtg
    20581 ccgagcagcg acagcgagaa cgccgagatc gccagcagca gggtccagcc ggcgaagctg
    20641 ccgcgcttct cggtgaccgc ctgcgaatgc agcaacgcgg tgccggccag ccacggcatg
    20701 aagctggcgt tctcgaccgg atcccagaac caccagccgc cccagcccag ttcgtaatag
    20761 gcccaatagg cgccaagcgt gatgccgatg gtgaggaacg accacgccgc cagcgcccaa
    20821 ggccgcgcgg ccttcgccca ggctttcggt tcggcgcgcg accacaggcc cgcgatcgcg
    20881 taggaataca caaccgacat gccgacatag cccatgtaga gaagcggcgg atggatcgcg
    20941 agcgccgggt cctgcagcag cgggttgaga tcgccgccgc gcaagggcgc gggatcgagc
    21001 cgcgcgaacg ggttcgacgc aaagatcaga taggcgaaag acaaggtcgc gatcacgccc
    21061 tgcaccgccg ccgcgcgggc gtcgagccag gactcgtcgc gtccatcctt gggcgcgcgc
    21121 gatgcggcgg cgaaaccgta gatgcacgcc accagacacc acatcagcat cgagccttcg
    21181 tggtttcccc aggc
//
LOCUS       DAOOCX010000034        21173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2175_length_21173_cov_7.882233, whole genome shotgun sequence.
ACCESSION   DAOOCX010000034 DAOOCX010000000
VERSION     DAOOCX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 21173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21173
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2175_length_21173_cov_7.882233"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="PK399_05020"
     CDS             <1..179
                     /locus_tag="PK399_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HPW12515.1"
                     /translation="SYHMMGVPTAMFTPLFVIARTSGWSAHVIEQRIDGKIIRPSANY
                     TGPEDREYVALDKR"
     gene            199..681
                     /locus_tag="PK399_05025"
     CDS             199..681
                     /locus_tag="PK399_05025"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HPW12516.1"
                     /translation="MDWLIPDGLFAEHALVAVVAALATALVVLVHYEGVQWLAKRYSG
                     RAVARATDRRAMLKIIFALLGLHIVEIWCFGLAYWGLLHVPDVGFVHGEHGSDNLFDA
                     IYLSATTYTTLGIGDLAPVGAVRLVSGMEALTGFLMITWSASFTYLEMSRLWTDEQRR
                     "
     gene            complement(778..1239)
                     /locus_tag="PK399_05030"
     CDS             complement(778..1239)
                     /locus_tag="PK399_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004154513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HPW12517.1"
                     /translation="MKLAWILWLSSNVLPQPAADSLCLSTTVYLEARDQPVRGQRAVA
                     EVALRRRDSGLWGQNVCEVVTARKQFAPTIVSPQTRLSNGEAWAEAVTIALESEHNWA
                     LPKGQRREIVPGASHFMAHAIASPSWRNAYQVATIGDHTFLQVQKLKSRRG"
     gene            complement(1519..3540)
                     /locus_tag="PK399_05035"
     CDS             complement(1519..3540)
                     /locus_tag="PK399_05035"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HPW12518.1"
                     /translation="MTNPSYPHLFQPLDLGFTRLRNRVLMGSMHTGLEDRAADFPKLA
                     AYFAERAAGGTGLIVTGGFAPNLVGWLKPFASRMSMPWHVARHRQVTSAVHAHDGKIC
                     MQLLHAGRYAYHPLSVAPSKLKAPINPFTPRALSARGIERHIRDFATAASLAREAGYD
                     GVEVMGSEGYLLNEFTSARTNRRTDAWGGDAAKRMRFAVEIVRQVREACGPDFILVYR
                     LSMLDLVEGGNGWDDIVAQAKAVEAAGATIINTGIGWHEARVPTIATSVPRAAFAGVT
                     AKLKPHVGVPLVATNRINMPDVAEAILASGGADMVSMARPLLADPEWVNKARTGRSNA
                     INTCIACNQACLDHVFEQKKASCLVNPRACAETELNYTKTALPKSIAVIGAGPAGLAC
                     ATVASERGHRVTVFDAADRIGGQFNLAKTIPGKEEFHETLRYFGQRIEDTGVTLRLST
                     RVDADGLAGFDEVVVATGVTPRSVDIEGHDHPKVVSYLDVLERRVIPGAKVAIIGAGG
                     IGFDVAEFLVETGPSATTDPARWMAEWGVDANFETAGGLVTAHPEPPARKVWLLQRSA
                     GKPGARLGKTTGWIHRATLKAKGVKMLGGVEYLGVDDAGLHVRVDGSEQTLPVDHVVI
                     CAGQESKRGLFDALQAKGQRSHLIGGANVAAELDAKRAIAEGSKLAASL"
     gene            3896..4906
                     /locus_tag="PK399_05040"
                     /pseudo
     CDS             3896..4906
                     /locus_tag="PK399_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017171142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
     gene            5108..5617
                     /locus_tag="PK399_05045"
     CDS             5108..5617
                     /locus_tag="PK399_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12519.1"
                     /translation="MLSIQRVSSILLLLAVPAGVAHAAGEACHGDALSATPVRPTLIA
                     PVAIELAPATTQLGGATGVLSQSFDEALSVDNVLLRIRLDGCRAMAKANIPSGVAKPG
                     DPAAYKPRTEFDNAPWRFDMTQNGKRMTADEFDAWMKARGVRVVGRKDAPAAEVAAPP
                     AAPADKPKK"
     gene            5664..>6258
                     /locus_tag="PK399_05050"
     CDS             5664..>6258
                     /locus_tag="PK399_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW12520.1"
                     /translation="MGLARVLSKRGLCSRTQAAEWIRSGRVRVDGRVVRDPEFPTVAG
                     KHRIAVDGRGLDEVPGARLHLMLNKPRGLVTTLRDEQGRDTVYRCFDGAGLGFIAPVG
                     RLDKASEGLLLFTNDPEWAARITSPDAGPDKTYHVQADCIPDAAMLATMQAGIDVEGE
                     WLRAKSVVVLRAGEKNAWLEVVLDEGRNRQIRRLLAAF"
     assembly_gap    6259..6358
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6359..7399)
                     /gene="kbl"
                     /locus_tag="PK399_05055"
     CDS             complement(<6359..7399)
                     /gene="kbl"
                     /locus_tag="PK399_05055"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPW12521.1"
                     /translation="MSLTQPALTQRYADTLDEIRAAGLFKSERIITSPQSAEIELADG
                     RTVLNFCANNYLGLADHPDIIAAAKDALDSHGFGMASVRFICGTQDLHKQLERTIAGF
                     FGTEDTILYAACFDANGGLFEPLLDEHDAIISDALNHASIIDGVRLCKARRYRYANCD
                     MADLEAQLKQAKADGARTIMITTDGVFSMDGFIAPLDEITTLAAKYGALVHIDECHAT
                     GFLGATGRGSAEVKGVMEKIDIFTGTLGKAMGGALGGFTTAKKEVIELLRQRSRPYLF
                     SNSLPPHVVAAGIKAFEMLSSAGELRERLRENTAHFRTRMVDAGFDIRPGVHPISPVM
                     LYDAPLAQRFAER"
     gene            complement(7408..8430)
                     /gene="tdh"
                     /locus_tag="PK399_05060"
     CDS             complement(7408..8430)
                     /gene="tdh"
                     /locus_tag="PK399_05060"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010378413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HPW12522.1"
                     /translation="MKALVKRETGKGIWMEQVPVPVPGPNEVLIKLEKTAICGTDLHI
                     YLWDEWSQRTIQPGLVIGHEFVGRIVDLGPGVTGYQLGQRVSAEGHIVCGHCRNCRAG
                     KQHLCPNTVGIGVNRNGAFAEYIVMPASNLWPIPDQIPSELAAFFDPYGNAAHCALEF
                     DVVGEDVLITGAGPIGVIAAGICKHIGARNVVVTDVNDFRLKLAADMGATRVVNVANT
                     SLKDVMADLHMEGFDVGLEMSGNPRAFNDMLDCMYHGGKIAMLGIMPKGAGADWDKII
                     FKGLTIHGMYGRKMYETWYKMTQLVLSGFPLGKVLTHQLPIDDFQKGFDLMEGGKSGK
                     VVLSWN"
     gene            8584..10734
                     /locus_tag="PK399_05065"
     CDS             8584..10734
                     /locus_tag="PK399_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPW12523.1"
                     /translation="MRHRTLPVVVALILAGGAAHADEGMWMPSQLPQLAKQLKAAGFK
                     GNPADLADLTKPPMSAVVSIGGCTASFVSPQGLVVTNHHCALGAIQLNSTAERNLIQD
                     GFIAASMADEPSAGPAARIWVTVGFDPITDRILADARGKTGRAYYEAVETAQKAAVAA
                     CEQDAGHRCSVASMYYGREYYLIKQLELRDVRLVYAPPRAIGNYGDEIDNFMWPRHTG
                     DFTFYRAYVGKDGKPAAYSKDNVPYAPPAHLTVSTAPVKAGDFAMVAGYPGTTFRHRT
                     ASEFANQIDWLLPERVDMVGGLIRTIETATAGDKAKEVLYASAVAGNKNTLKRAQGEL
                     DGLRRSDAVRVRREDETAMLAWLAKQPDAATLQADIRAMQAQLDAGQATRERDFLLPF
                     LRPGLLGSAITLRRLAMERGKPDAQRESGFQQRDEVLIEGGLKQLQRRYDPEVEKATL
                     RYALKRYYALPASQRLNEFDAAFGANETIASAKLDSLYAQTKLGVEADRLALMKASPD
                     AVLASTDPLMQLAAALQPALLRLEEEGKQRSGELMRLRPAYMQALIGYRESQGRAVYP
                     DANSTLRVSYGKVSPLSPRDGLDYRPLTTVAGIVEKNTGVEPFDAPKPLLDAIAAGDF
                     GSTIDPTLKTQTVDFMTNLDTTGGNSGSPVLDANGKLIGLNFDSNWEAVSASWMFDPR
                     YKRAIHVDMRYLRWLLAKVYPAPHLLKEMNLPAE"
     gene            10784..>12912
                     /locus_tag="PK399_05070"
     CDS             10784..>12912
                     /locus_tag="PK399_05070"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPW12524.1"
                     /translation="MQKTMLAAAIAALMMGTASTANADEGMWVPQQLPEIAGPLKKAG
                     LKLDPKQLADLTGDPMGAVVSLGGCTASFVSPQGLVVTNHHCAYGAIQLNSTAERNLI
                     DLGFNAPTMADELSGGPNARIYVLDEITDVTAQVKAAIAAAPNALARTKAVDDLEKQL
                     VGACEADAGSRCRLYSFMGGNTYRLFKNIEIKDVRLAYAPPRGVGNYGGEVDNWMWPR
                     HTGDFSFYRAYVGKDGKPAAYSKDNVPFKPKHWLKIADQPLREGDFVMVAGYPGSTSR
                     YALAADFDATSTWTYPTTSRRFKELVAMVLAAGEANKDIEVKYANTVRGWENTLKNYD
                     GQMEGFARMGAAGIKREREQAVLAWLKSRGAEGAQALAAHDTLVALGDAARKTRERDG
                     VIGNVGGALGGTALTLYRLAVEREKPDAQRESGFQQRDLPGIEGGVKQMDRRYVASMD
                     RQLQRYWLMQYIALPADQRIAAIDAWIGGNDAKAVDAALDRINASKLGSLDERMKWLS
                     ADRKAFEASTDPAIQYAVAMLPTSLKLEEERKLRAGEAVIPRATYLQAVADYNKAQGK
                     AVYPDANSSLRITFGNVVSYTKPGASKPEDAFTTLEQVAAKATGKEPFDAPQALLDAI
                     KAKRYAGMADAKLKTVPVNFLSNLDITGGNSGSPVLDAHGKLVGLAFDGNWESVASNW
                     VFDPTVTRMISVDQRYMRWVMQEVMPAP"
     assembly_gap    12913..13012
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13013..13274)
                     /locus_tag="PK399_05075"
     CDS             complement(<13013..13274)
                     /locus_tag="PK399_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020684108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HPW12525.1"
                     /translation="MQAKGEFEVKRIPQDELDIGQGATVGHSRFDKRFHGPLEATSVV
                     HMLALMSPVQGSGAYVAMERIEGTLDGRRGSFYMQHNGIMDRG"
     gene            13382..14032
                     /locus_tag="PK399_05080"
     CDS             13382..14032
                     /locus_tag="PK399_05080"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPW12526.1"
                     /translation="MKILLARHGETPWNAEGRYQGQEDIPLSDVGIGQATALGERLRD
                     LPIQRAIASPLSRARRTAELALGAERAGQLMFDGGLKEIAHGEWEGLLASEIDERDGE
                     RLRAWRDAPETVQMPGEGGESLQQVLDRAWPAFARACAGLGADDTLLVVAHDAVNRVL
                     LCRILGIPLSKLWGFRQAPTTINLLEGASVDALEVVRLNDCSHHTALFGEAKHRAL"
     gene            complement(14036..14893)
                     /locus_tag="PK399_05085"
     CDS             complement(14036..14893)
                     /locus_tag="PK399_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPW12527.1"
                     /translation="MSQPAPILGSMRFGSWGADLSPGAVASLLEAALDAGIDTLDLAD
                     IYGDHATNPLIGDALASRPGLRQRLNLLAKVGIVRSSSPGNPRGVQHYDLSVRHLRSA
                     LDTSLRDLQTDHVDVLMLHRFDPLMEPAAIADWVREEQVAGRVGAFGVSNFGAHALAL
                     FDGKLAIVGNQVELSLANSNVIDDGTLDATRARGADVQAWSPLGGGGLLQPPAALGDV
                     LRSLGGEFGLDTASLLLRWVASVPGTRVVIGSTDAARIRAAADACATPLPKDAWYALW
                     EAARGFPVP"
     gene            14937..16205
                     /gene="folC"
                     /locus_tag="PK399_05090"
     CDS             14937..16205
                     /gene="folC"
                     /locus_tag="PK399_05090"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HPW12528.1"
                     /translation="MRTRPLSDWLAYIEQQHPKSIAMGLERVREVATRMGLGKPAKRV
                     ITVGGTNGKGSTVAFIEAIARAHGWKTGAYTSPHLLRYNERVRIDGADVEDDVLAEGF
                     EAVEAARGETPLTYFEYGTLCALWLFARAKLDLVVLEVGLGGRLDAVNIVDADVAVIT
                     TVDLDHQAWLGDNIESIGFEKAGIARPFKPLVLGDDDPPASVLRHAYAIGAQSWRIAN
                     DFFAEPLDATTWRWREVGHAMTLPRPALDAPVQLRNAACAVAALRALPGRIERQAYVE
                     GIANAQVVGRLQRFRRDGVDVVVDVGHNPQAARALAMWLRAQPRRRTIAVYAALADKD
                     AAGVVSALAAQVDAWQLAGLADAGPRGQDAAALRQRLAGTEAATAEAHETPVAALEAA
                     LQAAGADGRVLVFGSFHVTAVALRWLNDVA"
     gene            16267..17277
                     /locus_tag="PK399_05095"
     CDS             16267..17277
                     /locus_tag="PK399_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPW12529.1"
                     /translation="MDTSLKQRLVGAVVLVALAVIFLPMLVKGPAPESGVSDVALDVP
                     AEPATGDGMVTRDLPLVAPAGAPQGGATGMPSSMPDAAPAPSAQDGAFPATAAGDHAV
                     SFGSYATAADADKVIAALKAAELPGYRETVTLNGRQAERVRIGPFADLAVAESARLRA
                     AQVNPNVGAKVIALDANPAASPLPASAPSTAVPASPPAPAKSEPLPPAAAKPVVPATT
                     PAAKPAVVADAKPATPVAKPPATTPPAARPEPASKPADTSKTGFAVQVGAFADAAAAT
                     ALRDKLRSAGFNAFTDNVSTDGGTRTRVRVGPAMNRAEADALKAQVKAKAGIDGIVRP
                     HP"
     gene            17311..18780
                     /gene="purF"
                     /locus_tag="PK399_05100"
     CDS             17311..18780
                     /gene="purF"
                     /locus_tag="PK399_05100"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPW12530.1"
                     /translation="MCGIVGIVGHSEVAAQLYDGLTVLQHRGQDAAGIATADGGKLRV
                     HKGNGLVRDVFDEPAMALLSGRVGVAHCRYPTAGSEGSDEAQPFYVNSPFGIALAHNG
                     NLINTDALRREVFEQDRRNVNTESDSEVLLNVFAHELDLQKALTPGAVFRALEGVNRR
                     AKGGYAVVCAVLGLGLVAFRDPHGIRPLVLGKRVAAGGDEYIVASESVALDILGFERL
                     RDVEPGEGIVITARGELFAQQCAQPQEHAPCIFEYVYFARPDSMIENISVHKARMRMG
                     VTLGEKILRERPDHDIDVVIPIPDTSRDSALEIANVLGVKYREGFIKNRYVGRTFIMP
                     GQGERAKSVKRKLNPIPLEFRNRVVLLVDDSIVRGTTSKQIVQMARDAGAKKVYLASA
                     APPVRYPNIYGIDMPSSEELVAHGRSEKDIEQLLGCDWLIYQELSDLEAAVAGPKFPG
                     RRFDSSCFSGEYVTGIEAGYFERLQQLRSDDAKKTRRAS"
     gene            18883..19686
                     /locus_tag="PK399_05105"
     CDS             18883..19686
                     /locus_tag="PK399_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017115654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPW12531.1"
                     /translation="MTSLFDAARACLDAASPDKKVACTFATAAAFARGESIIDADAPP
                     PEAIRMPGRPERPLLVHPRDLPRRGFGSVEGRAAFIHAVAHIEFNAIDLAWDAVYRFR
                     GMPAAYYADWVSVANDEARHFAMLRARLKELGHDYGDFDAHNGLWEMAEKTAGSGLAR
                     MALVPRVLEARGLDVTPGMIVKLRSLGDDVTADILDIILREEVAHVAAGSRWYRWHCG
                     REGVEPRGRFRELLREYATGVLHKPFNTQARLEAGFDLEELESLLAEAG"
     gene            19748..20188
                     /locus_tag="PK399_05110"
     CDS             19748..20188
                     /locus_tag="PK399_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12532.1"
                     /translation="MKFALASLSLLLVACQPSPPPAVAPAAPAPPAAEAPSVKRAPGQ
                     ENVDPLQVFRAFGTEPFWGVNVEGTRLTYTTPDDQAGVVMEGTRKTLADGVEISGQHD
                     GKPFVLTVGSGTCSDGMSDNTYSLVATFRFGEIDYTGCGEAAKL"
     gene            complement(20207..20929)
                     /locus_tag="PK399_05115"
     CDS             complement(20207..20929)
                     /locus_tag="PK399_05115"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPW12533.1"
                     /translation="MTTLFIADLHLDPERPAITALFGRFIEEEAADADALYILGDLFE
                     AWVGDDDPSDAGAFVADKLHALAASGVPVHFIRGNRDFLLGAGYAARCGMSLLDEPSV
                     IDLYGTPTVILHGDLLCTDDPAYQQFRAQTRDPRWQAQFLSQPLEARLAFAAQARAAS
                     KARYGELVSAGTAEIVGDVAKNAVDALFATSGVGRMIHGHTHRPAIHDEGNGTTRIVL
                     GDWYEQGSVLRVDPDGVELASL"
     gene            21110..>21173
                     /locus_tag="PK399_05120"
     CDS             21110..>21173
                     /locus_tag="PK399_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPW12534.1"
                     /translation="MTVRTRFAPSPTGYLHIGGAR"
BASE COUNT         3463 a         6923 c         7189 g         3398 t
ORIGIN      
        1 tcagctacca catgatgggc gtgcccaccg cgatgttcac cccgctgttc gtgattgcgc
       61 gcaccagcgg ctggagcgcg cacgtgatcg agcagcgcat cgacggcaag atcatccgcc
      121 ccagcgcgaa ctacaccggt cccgaggatc gcgaatacgt ggcgctcgac aagcgttgag
      181 catcacgcgg agcgcggcgt ggattggctg atccccgacg gattgttcgc ggagcatgcg
      241 ttggtcgccg tggtggccgc gctggccacg gcactggtgg tgctcgtcca ttacgaaggc
      301 gtgcagtggc tggcgaagcg ctattcgggt cgcgccgtcg cgcgggcaac cgatcgccgc
      361 gcgatgttga agatcatctt cgcgctgctg ggcctgcaca tcgtcgagat ctggtgcttc
      421 ggcctggcct actgggggct gctgcacgtg ccggacgtcg gcttcgtgca cggcgagcac
      481 ggctcggaca atctgttcga tgccatctac ctgtcggcga cgacctacac gacactcggc
      541 atcggcgacc tggcgccggt cggcgcggtg cgtctggtgt ccggcatgga agcgctcacc
      601 gggttcctga tgattacctg gtccgcgtcg ttcacctatc tcgagatgtc gcggctgtgg
      661 acggatgagc agcgtcgctg agcgacgcgc tgggaccatg aagaagccgc gcactgcgcg
      721 gcttcttcat gggtggacag ggtggcctgg cctgccgttg caggccgctg gatcagctca
      781 accgcgacgc gacttgagct tctgcacctg caggaaggtg tggtcgccga tggtcgccac
      841 ctggtaggca ttccgccaac tcgggctggc gatcgcgtgc gccatgaagt ggctggcacc
      901 gggcacgatc tcgcggcgtt ggcccttggg tagcgcccag ttgtgttcgg attccagcgc
      961 gatggtgacg gcttcggccc aggcttcgcc attcgacagc cgtgtctggg gcgagacgat
     1021 ggtgggcgcg aactgcttgc gcgcagtcac cacctcgcag acgttctgcc cccacaagcc
     1081 actgtcgcgg cggcgcaagg ccacctcggc aacggcgcgt tggccgcgta ccggctggtc
     1141 gcgcgcctcc aggtagacgg tggtggacag gcacaacgaa tctgcggcgg gctgcggcag
     1201 cacgttgctg gatagccaga ggatccaggc cagtttcatg tcgaactcct tgctccgttg
     1261 cggcgcaggc gcttgcatcg gcggcaaccg atgggaacgc atgcgtcatg gcgtactggg
     1321 gagggcggca gctctatggc ggcctttgcc cgggcccgcc gcattcgtgc ggggggcggg
     1381 ttgcctgcct atgcgcgaag gctcgcgtcg gggcagggcg gaaagtgggc gcaccttagg
     1441 ggcgcttgcc ccaacgccag ctgaacagcg atttcggaag ccactgattc gattggattt
     1501 tttattcgga gaactgccct acaggcttgc tgcgagcttg ctcccctcgg cgatcgcacg
     1561 cttcgcatcg agctcggccg caacattcgc accgccgatc aggtggctgc gttgtccctt
     1621 ggcctgcagc gcatcgaaga gtccgcgctt cgattcctgg cccgcacaga tgaccacgtg
     1681 atcgaccggc aacgtctgct cgctgccatc gacgcgcacg tgcaggcctg cgtcgtcgac
     1741 ccccaggtat tcgaccccac ccagcatctt cacgcccttc gccttcagcg tggcacggtg
     1801 gatccagccg gtggtcttgc ccagccgcgc accgggcttt cccgccgagc gttgcagcaa
     1861 ccagaccttg cgcgccggcg gttccgggtg cgccgtcacc aggccgccgg ctgtctcgaa
     1921 gttggcatcc acgccccatt cggccatcca gcgggcgggg tccgtcgtgg ccgacgggcc
     1981 ggtttcgacc aggaactccg cgacgtcgaa gccgatgccg ccggcaccga tgatcgcgac
     2041 cttggcaccg gggatgacgc ggcgttccag cacgtcgagg tagctaacga ccttgggatg
     2101 gtcatggcct tcgatgtcga ccgagcgcgg ggtgaccccg gtggcgacca cgacctcgtc
     2161 gaaaccggcc aggccatccg catcgacccg ggtggacagt ctcagggtga cgccggtgtc
     2221 ctcgatgcgc tggccgaagt agcgcagggt ctcgtggaac tcttccttgc ccgggatcgt
     2281 cttggcgagg ttgaactggc caccgatccg atccgcggca tcgaacacgg tgacgcgatg
     2341 gccgcgctcc gaggcgacgg tggcgcaggc caggccggct ggaccggcgc cgatcacggc
     2401 gatggacttc ggcaaggccg tcttcgtgta gttcagttcg gtttccgcgc aggcgcgtgg
     2461 gttgaccagg caactggctt tcttttgttc gaacacatgg tccaggcagg cctggttgca
     2521 ggcgatgcag gtattgatgg cattgctgcg gccggtgcgg gccttgttca cccattccgg
     2581 gtcggccagc aacggtcgcg ccatcgacac catgtcggcg ccgccggatg ccaggatcgc
     2641 ctcggcgaca tcgggcatgt tgatccggtt ggtcgcgacc agcggcacgc cgacgtgcgg
     2701 cttcaacttc gcggtgaccc cggcgaacgc cgcgcgcggc accgaggtgg cgatggtcgg
     2761 tacgcgcgcc tcatgccagc cgatgccggt attgatgatg gtggcgccgg cggcttccac
     2821 cgccttggcc tgggcgacga tgtcgtccca gccgttgccg ccttcgacca ggtcgagcat
     2881 cgacaggcga tagaccagga tgaagtccgg cccgcaggcc tcgcgcacct ggcgcacgat
     2941 ctccaccgcg aagcgcatcc gcttggcggc atcgccaccc caggcatcgg tgcgccggtt
     3001 ggtgcgcgcc gaggtgaact cgttcagcag gtagccctcc gagcccatca cctcgacgcc
     3061 gtcatagccg gcctcgcggg ccaggctcgc ggcggtggcg aagtcgcgga tgtggcgttc
     3121 gatgccgcgc gccgagaggg cgcgtggggt gaacgggttg atcggtgcct tcagcttcga
     3181 gggcgccacc gacagcgggt ggtaggcata acgccccgca tgcagcagtt gcatgcagat
     3241 cttgccgtcg tgtgcatgca ccgccgaggt gacctgcctg tgccgggcga cgtgccacgg
     3301 catcgacatg cggctcgcga agggcttcag ccagcccacc aggttcggcg cgaatccgcc
     3361 ggtcacgatc aggccggttc cccccgcggc acgttcggcg aaataggccg ccagcttcgg
     3421 gaagtcagcc gcccggtctt caaggccggt atgcatcgaa cccatcaata cccggttccg
     3481 caggcgggtg aaacccaggt cgaggggttg gaacaggtgc gggtaggaag gattggtcat
     3541 ggcacaagca tactcatgcg tacggtcatg tgcatggcct tgtcgggtgg cggcgccggg
     3601 tcgatgccgc atgatcatgc cgggcaggat gcaaccgtgc catacgtcac gatccgacgt
     3661 gctagatttg cgtgtcggcc gtgccagtcg ggaggggtgc cggcagcatc tgcaggcatg
     3721 tatcaggtgc gggtgaaccg gcctccgttt tgcatcgtga caactgttat gctagtgtta
     3781 acacggcttt cacgtggccg ccattactgt acgcgggatg gcgtgcggac tcagccgtct
     3841 gaagcacatc acaatgtggc gtcacaagtt tctattactg atcaaggagc tgaacatgaa
     3901 taagaaactc ctctgcgctg ccctgctggg tagtctgggt ctggcgcagg ctgcgtccgc
     3961 ccaggagttt gatgatcgct ggtatgtctc cggctcgacc ggcgtcaatt tccaggacaa
     4021 ggatcgcgga accgaagact cgatgttcgt gaccatgggc ttcggcaagt tcctcaaccc
     4081 gaacctgtcg ctggaagcgg aactgaacta tcagaacccc gacaagacgg tcaactccag
     4141 cctgtggtgg agccagtacg gcgcctccgt ggatgcgcgt taccacttcc gcaatgcgga
     4201 ctcgaagtgg tggccctacc tgcgcggcgg tatcggctgg cagcgccacg aggaagaata
     4261 cgacgccttc ccgaacccga attccccggg ccagcatgaa gactccaacc tcgccctgaa
     4321 cctgggcgca ggcctgcagt tcgatttcgg ccgcgtcgat ctgcgtaccg aagtcggtac
     4381 ccgcgtcgat ttcgatgacg tgcaggcccg catcggctcg caggaagaca tgttcaccga
     4441 cctgctcgca tcggttggcc tgaccgtggc cctcgggccg gagccggttg ccccggttgc
     4501 cccggcaccg gtcgccgaag ccagttgcgc ggacatggat gacgacggtg acggcgtcaa
     4561 caactgcaac gacaagtgcc cgggttcgca ggctggccag accatcggtc cggatggctg
     4621 cccggtgccg gtctcgatcg acctgaaggg cgtgaacttc gacttcgaca agtcgaccct
     4681 gcgtccggat gccgtcgcga tcctcaacga ggccgtcgaa atcctgaagc gctatcccga
     4741 cctgcgcgtc gaagtcgccg gtcacaccga cctgtgcggc accgacgtgt acaaccagtc
     4801 gctgtcggag cgtcgcgcca aggccgtgta tgactacctg accggcaatg gtgtcgacgg
     4861 cggccgcctg gccggcccga tcggctacgg cgagagccgt ccgctggagc agaccgcgca
     4921 gacgctgccg ggttgcaaga gcgagaccaa ccgccgcacc gagctgaacg cccagtaatc
     4981 ctggtcgcca gcgatgcaat acgcgaagcc cggcctagtg ccgggcttcg tctttctggc
     5041 aacgcaggat ccgattgcgg cattcctgcg ccagcaccta cactgctcgc tcagtcgggg
     5101 agtcatcatg ctgtcgatcc aacgtgtgtc gtccatcctg ttgctgctgg ccgttcctgc
     5161 cggcgtcgcg catgctgcgg gcgaggcctg ccatggcgat gcgttgtcgg cgacgcccgt
     5221 gcgaccgacg ttgatcgcgc ccgtcgcgat cgagctggcg cctgccacga cccagctggg
     5281 gggggcgacc ggcgtgctgt cgcagtcgtt cgacgaagcc ttgtccgtcg acaacgtgct
     5341 gctgcggatc cgcctggatg gttgccgcgc gatggcgaag gcgaatatcc cgtcgggcgt
     5401 ggccaagccg ggtgatccgg cggcttacaa gccgcgtacg gagttcgaca acgcgccctg
     5461 gcgtttcgac atgacccaga acggcaagcg catgactgcc gatgaattcg acgcctggat
     5521 gaaggcacgc ggcgtgcgcg tggtcggccg caaggatgcg cctgccgccg aggtcgccgc
     5581 accaccggct gcgcctgcag acaagccgaa gaagtaaccc cgcggctggt cggagtcacg
     5641 acgtggccga tgccgcgcgc aggatgggat tggcccgcgt gctctccaag cgcggcctgt
     5701 gctcgcgcac gcaagccgcc gaatggatcc gcagcggccg agtccgggtc gacggtcgcg
     5761 tagtgcggga tcccgaattc ccgacggtcg ccggcaagca ccggatcgca gtggatggac
     5821 gcggcctcga cgaggtcccg ggcgcgcgtc tccatctcat gctcaacaag ccccgcggtc
     5881 tggtcacgac cctgcgcgac gaacagggcc gcgataccgt ctatcgttgc ttcgatggcg
     5941 ctggcttggg cttcatcgcg cccgtgggcc gcctcgacaa ggccagcgaa gggctgctcc
     6001 tgttcaccaa cgatcccgag tgggctgcgc gcatcacgtc gcccgacgcc gggcccgaca
     6061 agacctacca cgtgcaggcg gactgcattc cggatgcggc gatgctcgcg acgatgcagg
     6121 caggcatcga tgtcgaaggc gagtggttgc gggcgaagtc ggtggtcgtg ctgcgcgccg
     6181 gcgaaaagaa cgcctggctg gaggtcgtgc tggacgaagg ccgcaaccgg cagatcaggc
     6241 ggctgctcgc ggcattcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     6361 gttcggcgaa acgctgcgcc agcggcgcgt cgtacagcat caccgggctg atcgggtgca
     6421 cgccggggcg gatgtcgaag ccggcatcga ccatgcgcgt gcggaaatgg gcagtgttct
     6481 cgcgcagtcg ttcgcgcaat tcaccggcag acgacagcat ctcgaacgcc ttgatgcccg
     6541 cggcgaccac gtgcggcggc aacgagttgg agaacaggta ggggcgcgaa cgctggcgca
     6601 gcagttcgat gacttccttc ttcgcggtgg tgaacccgcc gagcgcaccg cccatcgcct
     6661 tgcccagcgt accggtgaag atatcgatct tttccatcac gcctttcacc tcggccgaac
     6721 cgcggccggt ggcgccgagg aacccggtgg cgtggcattc gtcgatgtgc accagcgcgc
     6781 cgtacttcgc ggcgagcgtg gtgatctcgt ccagcggcgc gatgaagccg tccatcgaga
     6841 acacgccgtc ggtggtgatc atgatcgtgc gcgcaccgtc ggccttcgcc tgcttgagct
     6901 gcgcttccag gtcggccatg tcgcagttgg cgtagcggta gcgcctggcc ttgcacaggc
     6961 gcacgccatc gatgatcgag gcgtggttga gcgcatcgct gatgatcgcg tcgtgctcgt
     7021 cgagcagcgg ttcgaacagg ccgccattgg catcgaagca ggcggcgtag aggatggtgt
     7081 cctcggtgcc gaagaagccg gcgatggtcc gctcgagttg cttgtgcagg tcctgggtac
     7141 cgcagatgaa gcgcacgctg gccatgccga agccgtgcga gtcgagtgcg tccttggccg
     7201 ccgcgatgat gtccgggtgg tcggccaggc ccaagtagtt gttggcgcag aagttcagca
     7261 ccgtgcggcc atcggcgagc tcgatctcgg ccgactgcgg actggtgatg atgcgttcgg
     7321 acttgaacag gccagcggcg cggatctcgt cgagggtatc ggcgtagcgc tgggtcaggg
     7381 caggctgggt cagggacatg tcggctctca gttccagctc aacacgacct tgcccgactt
     7441 gccgccttcc atcaggtcga agcccttctg gaaatcgtcg atcggcaact ggtgggtcag
     7501 caccttgccg agcgggaagc cgctcagcac cagctgggtc atcttgtacc aggtctcgta
     7561 catcttccgg ccgtacatgc cgtggatggt gaggcccttg aagatgatct tgtcccagtc
     7621 ggcgccggcg cccttgggca tgatcccgag catcgcgatc ttgccgccgt ggtacatgca
     7681 gtcgagcatg tcgttgaagg cgcgcgggtt gccgctcatt tccaggccca cgtcgaagcc
     7741 ctccatgtgc aggtcggcca tcacgtcctt gagcgaagtg ttggcgacgt tgaccacgcg
     7801 cgtcgcgccc atgtcggcgg ccagcttcag gcggaaatcg ttgacgtcgg tgaccaccac
     7861 gttgcgcgca ccgatgtgct tgcagatgcc ggcggcgatc acgccgatcg ggccggcgcc
     7921 ggtgatcagc acgtcttcgc cgaccacgtc gaactccagc gcgcaatgcg cggcattgcc
     7981 gtagggatcg aagaacgccg cgagttcgct cgggatctgg tccgggatag gccacaggtt
     8041 cgacgcgggc atcacgatgt attcggcgaa cgcgccattg cggttcacgc cgatgccgac
     8101 cgtgttcggg cacaggtgct gcttgccggc gcggcaattg cggcaatgcc cgcagacgat
     8161 gtggccctcg gcgctgacgc gctggccaag ctggtagccg gtcacgcccg ggcccaggtc
     8221 gacgatgcgg ccgacgaact cgtggccgat gaccaggcca ggctggatgg tgcgctggct
     8281 ccactcgtcc cacaggtaga tgtgcaggtc ggtgccgcag atcgcggtct tctcgagctt
     8341 gatcagcacc tcgttggggc cgggcaccgg caccggcacc tgttccatcc agatgccctt
     8401 gccggtctcg cgcttgacca gcgccttcat cgtttgcgtc tgcgacatgg cggatgcgcc
     8461 ttgcgggatc gaggcgcgca ttctaccgcc gctggcctgt gcggcaggtc atgaccgcag
     8521 gcacacgcca gcaccacgct tccagtggtg taatcggcat ttgaccgctc tgcaggagtt
     8581 tccatgcgcc accgcacgtt gcccgtcgtc gttgccctca tcctcgccgg tggtgcggcc
     8641 catgccgacg agggcatgtg gatgccgtcg cagttgccgc aactggcgaa gcagttgaag
     8701 gcagcgggct tcaagggcaa tccggccgac ctggccgacc tgaccaagcc cccgatgagt
     8761 gcggtcgtgt ccattggcgg ctgcacggcg agcttcgtgt cgccgcaggg cctggtggtg
     8821 accaaccacc attgcgcgct gggtgcgatc cagctgaatt ccaccgcgga acgcaacctg
     8881 atccaggatg gtttcatcgc cgcgagcatg gccgatgaac cttcggccgg gccggcggcg
     8941 cggatctggg tcacggtcgg cttcgacccg atcaccgacc gcatcctggc cgatgccagg
     9001 ggcaagaccg gccgtgcgta ctacgaggcg gtcgagaccg cacagaaggc ggccgtcgcg
     9061 gcttgcgagc aggacgccgg ccatcgctgc agcgtggcca gcatgtacta cggccgcgag
     9121 tactacctga tcaagcagct cgaactgagg gacgtgcgcc tggtgtatgc gccgccccgc
     9181 gcgatcggca actacggcga cgagatcgac aacttcatgt ggccgcgcca caccggcgac
     9241 ttcaccttct accgtgctta cgtgggcaag gacggcaagc cggcggcgta cagcaaggac
     9301 aacgtgccgt atgcgccacc cgcgcatctc accgtgtcca ctgcgccggt gaaggcgggc
     9361 gacttcgcga tggtcgcggg ttatcccggc acgacgttcc gtcaccgcac ggcgtccgaa
     9421 ttcgccaacc agatcgactg gctgttgccg gaacgggtgg acatggtcgg cggcctgatc
     9481 aggaccatcg aaaccgcgac tgcgggcgac aaggccaagg aagtgctgta tgcctccgcc
     9541 gtggccggca acaagaacac gctcaagcgt gcgcagggcg agctcgacgg cttgcgccgc
     9601 agcgatgcgg tgcgcgtgcg ccgcgaggac gaaaccgcga tgctggcctg gttggcgaag
     9661 cagccggatg ccgcgacgtt gcaggccgac atccgcgcga tgcaggcgca gctggacgcg
     9721 gggcaggcca cgcgcgagcg cgacttcctg ctgccgttcc tgcgccctgg cctgctcggt
     9781 tccgcgatca ccctgcgccg gctggcgatg gagcgcggca agccggacgc gcagcgcgag
     9841 tccggtttcc agcagcgcga cgaggtgctg atcgaaggcg gcctcaagca gttgcagcgt
     9901 cgctacgatc cggaggtcga gaaggccacg ctgcgttacg cgctgaagcg ctactacgcg
     9961 ctgccggcgt cgcagcgcct caatgagttc gatgcggcgt tcggtgccaa tgaaacgatc
    10021 gccagcgcga agctcgacag cttgtacgcg cagacgaagc tcggcgtcga ggcggatcgc
    10081 ctggcgttga tgaaagcctc gcccgacgcg gtgctggcat cgaccgatcc gctgatgcaa
    10141 ctggccgcgg cgctgcagcc ggcgctgctg cggctggagg aagagggcaa gcagcgcagc
    10201 ggcgaactga tgcgtctgcg gcctgcctac atgcaggcct tgatcggtta tcgcgaatcg
    10261 caaggcaggg cggtgtatcc ggatgcgaat tccaccttgc gcgtcagcta cggcaaggtc
    10321 agcccgctga gcccgcggga cggcctggac tatcgcccgc tgaccacggt cgccggcatc
    10381 gtcgaaaaga acaccggcgt ggaaccgttc gatgcgccga agccgttgct cgacgcgatt
    10441 gccgcaggcg acttcggcag caccatcgac cccacgctga agacccagac ggtcgatttc
    10501 atgaccaacc tggataccac cggcggcaat tccggctcgc cggtgctgga tgcgaacggc
    10561 aagctgatcg gcctgaactt cgacagcaat tgggaagccg tcagcgccag ctggatgttc
    10621 gacccgcgct acaagcgcgc gatccacgtc gacatgcgtt acctgcgctg gttgctggcc
    10681 aaggtgtatc cggcgccgca cctgctgaag gaaatgaacc tgccggcgga gtgatccccg
    10741 gtagcgaatg acgtaccacg cacacgaacg acaggacagg acgatgcaga agacgatgct
    10801 tgccgcggcg atcgccgcac tgatgatggg aacggcgtcc accgcgaatg ccgacgaagg
    10861 catgtgggtg ccgcagcagt tgccggagat cgccggcccg ctgaagaaag ccggcctgaa
    10921 actggacccg aagcagttgg ccgacctcac cggcgacccg atgggcgcag tggtgtcgct
    10981 gggcggctgc acggccagct tcgtctcgcc acagggcctg gtggtcacca accaccactg
    11041 cgcgtacggt gcgatccagt tgaactccac cgccgagcgc aacctgatcg acctcggctt
    11101 caacgcgccg accatggccg atgagctctc cggcggcccg aacgcgcgca tctacgtgct
    11161 cgacgaaatc accgacgtca ccgcgcaggt caaggccgcg atcgccgccg cgccgaatgc
    11221 gctggcgcgc accaaggccg tcgatgacct cgagaagcaa ctggtcggcg cttgcgaagc
    11281 cgacgccggc tcgcgctgcc gcctgtacag cttcatgggg ggaaatacct atcggctgtt
    11341 caagaacatc gagatcaagg acgtgcgcct ggcctacgcg ccgccgcgcg gcgtgggcaa
    11401 ttacggcggc gaggtcgaca actggatgtg gccgcgccac accggcgact tcagcttcta
    11461 ccgcgcctac gtgggcaagg acggcaagcc ggccgcgtac agcaaggaca acgtgccgtt
    11521 caagccgaag cactggctga agatcgccga ccagccgctg cgcgagggcg acttcgtgat
    11581 ggtcgccggt tacccgggca gcaccagtcg ctatgcgttg gccgccgatt tcgacgccac
    11641 ctcgacctgg acctatccga ccaccagcag gcgcttcaag gaactggtgg cgatggtgct
    11701 ggccgccggc gaggccaaca aggacatcga ggtcaagtac gccaacaccg tgcgcggctg
    11761 ggagaacacg ctcaagaact acgacggcca gatggaaggc ttcgcccgca tgggcgccgc
    11821 cggcatcaag cgcgagcgcg aacaggcggt gctggcttgg ctgaagtcgc gtggcgccga
    11881 aggcgcgcag gcgctggccg cgcacgacac cctggtcgca ctgggcgatg cagcgcgcaa
    11941 gacccgcgag cgcgacggcg tgatcggcaa cgtcggcggc gccttgggcg gcaccgcgct
    12001 gaccctgtat cgcctcgcgg tggaacgcga gaagcccgac gcccagcgcg agtccggttt
    12061 ccagcagcgc gacctgcccg gcatcgaggg cggcgtgaag cagatggatc gtcgctacgt
    12121 ggcgtcgatg gatcgccagc tgcagcgtta ctggctgatg cagtacatcg ccctgccggc
    12181 cgaccagcgc atcgccgcga tcgacgcgtg gatcggcggc aacgatgcga aggcggtgga
    12241 tgccgcgctg gaccggatca acgccagcaa gctgggcagc ctggacgagc ggatgaaatg
    12301 gctgtcggcc gaccgcaagg ccttcgaggc cagcaccgat ccggcgatcc agtacgcggt
    12361 ggcgatgttg ccgacctcgc tgaagctgga ggaggagcgc aagctgcgcg cgggcgaggc
    12421 ggtcatcccg cgcgccacct acctgcaggc cgtggccgac tacaacaagg cgcagggcaa
    12481 ggcggtgtat ccggatgcga attcgtcgct gcggatcacc ttcggcaacg tggtgagcta
    12541 taccaagccg ggagccagca agccggagga cgcgttcacc acgctggagc aggtggcggc
    12601 gaaggcgacg ggcaaggaac cgttcgacgc cccgcaggcg ctacttgatg cgatcaaggc
    12661 gaagcgttac gccggcatgg cggatgcgaa gctcaagacc gtcccggtca acttcctgtc
    12721 caacctcgac atcaccggcg gcaattccgg ttcgccggtg ctggatgcgc acggcaagct
    12781 ggtcggcctg gccttcgacg gcaactggga atcggtggcc tcgaactggg tgttcgatcc
    12841 cacggtcacg cgcatgatct cggtcgacca gcgctacatg cgctgggtca tgcaggaagt
    12901 gatgccggcg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgccgcga
    13021 tccatgatgc cgttgtgctg catgtagaag ctgccgcgac ggccgtccag cgtgccctcg
    13081 atgcgctcca tcgccacgta ggcgccggaa ccctgcaccg gcgacatcag tgccagcatg
    13141 tggaccacgc tggtggcctc cagcgggccg tggaagcgct tgtcgaagcg cgaatggccg
    13201 accgtggcgc cctggccgat gtccagttcg tcctgcggga tgcgtttgac ctcgaactcg
    13261 cccttggctt gcatgcgggg gatcctggga agcgggtgga gagcctaacg cctgcgccgt
    13321 aaaggcgcgt gcaacctgct aggctgttgc gacgcaacat agatgaacag gggccgctgc
    13381 catgaagatc ctgcttgcac gtcatggcga gacgccgtgg aacgccgaag gccgctacca
    13441 ggggcaagaa gatattcccc tgtcggacgt cggcatcggc caggccaccg ccctcggtga
    13501 gcgcctgcgc gatctgccca tccagcgtgc catcgcctcg ccgttgtcgc gcgcgcggcg
    13561 cactgcggaa ctggccttgg gcgcggagcg cgccggccag ctgatgttcg acggaggcct
    13621 gaaggaaatc gcccacggcg agtgggaagg cctgctggcc agcgagatcg acgagcgcga
    13681 cggcgaacgc ctgcgcgcct ggcgcgatgc gccggagacc gtgcagatgc cgggcgaggg
    13741 tggcgagtcg ctgcagcagg tgctggaccg tgcctggcct gcattcgcgc gtgcctgcgc
    13801 cggcctgggt gcggacgaca ccctgctggt ggtcgcccat gacgcggtca accgcgtgct
    13861 gttgtgccgg atcctcggca tcccgctgtc caagctatgg ggcttccgcc aggcgccgac
    13921 cacgatcaac ctgctggaag gcgcgtcggt cgatgcgctg gaagtggttc gcctcaacga
    13981 ctgcagccac cacaccgcgc tgttcggcga ggcgaagcac cgcgccctgt aaggttcagg
    14041 gcaccgggaa tccgcgcgcc gcttcccaca gcgcgtacca cgcatccttc ggcaacggcg
    14101 tcgcacaggc atccgccgcg gcacggatgc gcgcggcatc ggtgctgccg atgacgaccc
    14161 gcgtgcccgg cacgctggcc acccagcgca gcaacaacga cgcggtatcc agcccgaatt
    14221 cgccgcccag cgaccgcaac acatcgccca atgccgcggg cggttgcagc aggccgccgc
    14281 cacccagtgg cgaccatgcc tgcacgtcgg cgccgcgcgc gcgggtggca tcgagcgtgc
    14341 catcgtcgat cacgttgctg ttggccagcg acagttcgac ctggttgccg acgatggcca
    14401 gcttgccgtc gaacagggcc agcgcatgcg cgccgaagtt cgacacgccg aacgcgccga
    14461 cacgtcccgc cacctgttcc tcgcgtaccc agtcggcgat cgcggccggc tccatcagcg
    14521 gatcgaagcg atgcagcatc agcacatcca cgtgatcggt ctgcaggtcg cgcaggctgg
    14581 tgtccagcgc cgagcgcagg tggcgtaccg acaggtcgta gtgctggacg ccgcgcgggt
    14641 tgcccggact gctgctgcgg acgatgccga ccttggccag caggttgagc cgctgccgca
    14701 agccgggacg cgacgccaac gcgtcgccga tcagcggatt ggtcgcgtgg tcgccgtaga
    14761 tgtcggccag gtcgagggtg tcgatgccgg catccagcgc agcttcgagc agactcgcga
    14821 cggcgcctgg cgacaggtcc gcgccccaac tgccgaaacg catggagccg aggatcggcg
    14881 cgggctggct catgtcgggg tgtcgctcgg cggggacgag gccggataat agcggcatgc
    14941 gcacgcgtcc cctgagcgac tggctcgcct acatcgagca acaacatccc aagtccatcg
    15001 cgatgggcct ggagcgcgtg cgcgaggtgg ccactcgcat gggcctgggc aagccggcca
    15061 agcgcgtgat caccgtcggt ggcacgaatg gcaagggctc gaccgtcgcc ttcatcgagg
    15121 cgatcgcacg cgcgcatggc tggaagaccg gcgcctacac ctcgccgcac ctgctgcgct
    15181 acaacgagcg cgtgcgcatc gatggcgcgg atgtcgagga cgacgtgctg gcggaaggtt
    15241 tcgaggccgt cgaggctgcg cgcggcgaaa cgccgctgac ctatttcgaa tacggcacgc
    15301 tgtgcgcgtt gtggctgttc gcacgcgcaa agctcgacct ggtcgtgctg gaagtgggac
    15361 tgggtggccg cctggatgcg gtgaacatcg tcgatgccga cgtggcggtg atcaccaccg
    15421 tcgacctgga ccaccaggcg tggctgggcg acaacatcga gtcgatcggg ttcgagaagg
    15481 ccggcatcgc ccggccgttc aagccgctgg tgctgggcga cgatgatccg ccggccagcg
    15541 tgttgcggca tgcctacgcg atcggcgcgc aatcgtggcg gatcgccaac gatttcttcg
    15601 ccgaaccgct cgatgccacg acctggcgct ggcgcgaggt cgggcatgcg atgaccctgc
    15661 cgcggccggc gctggatgcg ccggtgcagt tgcgcaatgc ggcctgcgcg gtggcggcct
    15721 tgcgcgcctt gcccggccgc atcgagcggc aggcgtacgt ggagggcatc gccaacgcgc
    15781 aggtggtcgg tcgcctgcag cgctttcgtc gcgacggcgt ggacgtcgtg gtggacgtgg
    15841 gccacaaccc gcaggcggcg cgcgcgctgg cgatgtggct gcgcgcacag ccacggcgtc
    15901 gcaccatcgc ggtgtatgcg gcgctggccg acaaggacgc cgccggcgtg gtctctgccc
    15961 tggccgcgca ggtcgatgcc tggcaactgg ccggattggc cgatgccggg ccgcgcggcc
    16021 aggatgccgc tgccctgcgg cagcgcctgg ccggcacgga agccgccacg gcggaagcgc
    16081 acgaaacgcc cgttgccgcg cttgaagccg cgctgcaggc ggccggcgcg gacgggcgcg
    16141 ttctggtgtt cgggtccttc cacgtcaccg cggtggcgtt gcgctggctg aacgacgtgg
    16201 cctgagccgg gtgacgggcc ggtataattc cggctccccc gccgtcgtgg tcgtcccggt
    16261 atgccgatgg atacctcgtt gaagcagcgc ctggttggcg ccgtcgtcct ggtcgcgctg
    16321 gcggtgatct tcctgccgat gctggtcaag ggtcccgcgc ccgagagtgg cgtgtccgat
    16381 gtcgcgctgg acgtgcccgc cgaacctgcc accggcgatg gcatggtcac ccgcgacctg
    16441 ccgctggtcg cgcccgcagg tgcgccgcaa ggcggtgcga ccgggatgcc ctcgtcgatg
    16501 ccggatgccg cgccggcgcc cagcgcgcag gacggcgcat tcccggccac agccgccggt
    16561 gaccacgcgg tcagcttcgg cagctatgcc actgccgcgg atgccgacaa ggtgatcgcc
    16621 gcgctgaagg ccgccgaact gccgggctac cgcgaaaccg tgacgctcaa cggccgccag
    16681 gccgagcgcg tgcgcatcgg tccgttcgcc gacctggccg tggccgagtc cgcgcgcctg
    16741 cgtgcggcgc aagtgaatcc gaacgtcggc gccaaggtca ttgcgctgga tgccaatccg
    16801 gccgcgtcgc cgttgcctgc cagcgctcca tcgaccgcag tgcctgcatc gcctccggca
    16861 ccggcgaaat ccgaaccgct gccgcccgcc gccgccaagc ctgtcgtgcc ggctactacg
    16921 cctgcggcga agcccgctgt cgtggccgat gccaagccgg caacgcccgt ggcgaagccg
    16981 ccggcgacca cgccgccggc cgccaggccg gagccggcca gcaagcccgc cgacaccagc
    17041 aagaccggct ttgcggtgca ggtcggcgcg ttcgccgatg ccgctgcggc caccgccctg
    17101 cgcgacaagc tgcgcagcgc cggcttcaac gccttcaccg acaacgtcag caccgacggc
    17161 ggcacccgca cccgcgtacg cgtggggccg gcgatgaatc gtgccgaggc cgacgcgctg
    17221 aaggcgcagg tcaaggccaa ggccggcatc gacggcatcg tccggccgca tccgtaacac
    17281 cccaatcgtt cgagccagga aacaacccca atgtgcggca tcgtcggaat cgtcggccac
    17341 agtgaagtcg cggcccagtt gtacgacggg ctcaccgtgc tgcagcaccg tggccaggac
    17401 gccgccggca tcgccactgc cgatggcggc aagctgcgcg tgcacaaggg caatggcctg
    17461 gtgcgcgatg tattcgacga gccggcgatg gcgctgctgt ccgggcgtgt cggcgtggcc
    17521 cattgccgct acccgaccgc aggcagcgaa ggcagcgacg aggcgcagcc gttctacgtg
    17581 aactcgccgt tcggcatcgc gctggcccac aacggcaacc tgatcaacac cgatgcattg
    17641 cgccgcgagg tgttcgagca ggatcgccgc aacgtcaata ccgagtccga ttccgaagtg
    17701 ctgctcaacg tgttcgcgca cgaactcgac ttgcagaagg cgctcacgcc gggcgcggtg
    17761 ttccgcgcgc tggaaggcgt gaaccgccgc gccaagggcg gctatgcggt ggtctgcgcg
    17821 gtgctgggcc tgggcctggt ggcattccgc gatccgcatg gcatccgccc gctggtgctg
    17881 ggcaagcgcg tggcggcagg cggcgacgaa tacatcgtgg cctcggaatc ggtggcgctg
    17941 gacatcctcg gcttcgagcg gttgcgcgat gtcgaaccgg gcgagggcat cgtgatcacc
    18001 gcgcgcggcg agctgttcgc gcagcagtgc gcgcagccgc aggagcacgc gccctgcatc
    18061 ttcgagtacg tgtacttcgc gcggccggac tcgatgatcg agaacatctc ggtgcacaag
    18121 gcgcgcatgc gcatgggcgt gaccctgggc gagaagatcc tgcgcgagcg ccccgaccac
    18181 gacatcgacg tggtgatccc gatccccgac accagccgcg attccgcgct ggaaatcgcc
    18241 aacgtgctgg gcgtgaagta ccgcgagggc ttcatcaaga accgctacgt cggccgcacc
    18301 ttcatcatgc cgggacaggg cgagcgggcg aagtcggtga agcgcaagct caacccgatc
    18361 ccgctggaat tccgcaaccg cgtggtgctg ctggtcgacg attcgatcgt gcgcggcacc
    18421 accagcaagc agatcgtgca gatggcgcgc gatgccggtg ccaagaaggt gtacctggcg
    18481 tccgcggcgc cgccggtgcg ctatccgaac atctacggca tcgacatgcc gtcgagcgag
    18541 gaactggtcg cgcacggtcg cagcgagaag gacatcgagc agttgctggg ctgcgactgg
    18601 ctgatctatc aggaactctc cgacctcgaa gccgcggtgg ccggaccgaa attccccggt
    18661 cgccgcttcg acagttcctg cttcagcggc gagtacgtca ccgggatcga agccggttat
    18721 ttcgagcgcc tgcagcagtt gcgctcggat gacgcgaaga agacgcgaag ggcgtcgtaa
    18781 tttcgcgatg atgtatgcaa gcggggtccg atgggcctcg caaccaagcg agtatcgggg
    18841 agcgacttgc gaggcccatc ggatcccgct cgcgatgaca gcatgaccag cctcttcgac
    18901 gccgcccgcg cctgcctcga tgccgcatcg ccggacaaga aggtcgcgtg cacgttcgcc
    18961 acggccgcgg cgttcgcgcg cggcgaatcg atcatcgacg ccgatgcgcc gccccccgaa
    19021 gcgatccgca tgcccggccg cccggagcgt ccgctgctgg tgcatccgcg cgacctgccg
    19081 cgccgcggct tcggcagcgt cgagggccgc gccgcgttca tccatgccgt tgcccacatc
    19141 gagttcaacg ccatcgacct ggcctgggat gcggtgtatc ggttccgcgg gatgccggcg
    19201 gcgtattacg ccgactgggt ctcggtggcg aacgatgagg cccgccactt cgcgatgttg
    19261 cgcgcacgcc tgaaggaact cggccacgac tacggcgact tcgacgcgca caacggcctg
    19321 tgggaaatgg ccgagaaaac cgcaggttcc gggctggcgc ggatggcgct ggtgccgcgc
    19381 gtgctggaag cgcgcgggct ggatgtcacc ccggggatga tcgtgaagtt gcgctcgctg
    19441 ggcgatgacg tcactgccga tattctcgac atcatcctgc gagaggaggt cgcgcatgtc
    19501 gcggccggtt cgcgctggta tcgctggcac tgcgggcgcg aaggcgtcga accacgcggc
    19561 aggttccgcg agctgctgcg cgaatacgcc accggcgtgc tgcacaagcc gttcaatacg
    19621 caggcgcggc tggaagccgg cttcgacctc gaagaactgg aaagcctgtt ggccgaagcc
    19681 ggctgagttt catcgcggcc gaagatggca tgcggcacac tccgcgccat cagtccggag
    19741 cattgccatg aaattcgccc tcgcgtcgct atcgctgctg ctggtcgcct gccaaccgtc
    19801 gccaccgccc gccgtcgcgc cggctgcgcc tgcaccacct gctgccgaag cgccatcggt
    19861 caagcgcgcg ccaggacagg aaaacgtgga cccgctgcag gtcttccgcg cgttcggcac
    19921 cgagccgttc tggggcgtca atgtcgaagg cacgcgactg acctacacca cgccagacga
    19981 ccaggccggc gtggtgatgg aaggcacgcg caagaccctg gccgatgggg tcgagatctc
    20041 cggccagcac gacggcaagc ccttcgtgct caccgtcggc agcgggacct gcagcgatgg
    20101 catgtccgac aacacctaca gcctggtggc gacgttccgc ttcggcgaga tcgattacac
    20161 gggttgcggc gaagcggcga aattgtgagg tagcggcgtc ggtgtcctac aacgaagcga
    20221 gttcgacgcc gtccgggtcg acccgcagca ccgagccctg ttcgtaccag tcgccgagca
    20281 cgatgcgggt agtgccgttg ccttcgtcgt ggatcgccgg gcgatgggtg tggccgtgga
    20341 tcatccggcc cacgccagac gtggcgaaca gcgcatcgac ggcgttcttc gccacgtcgc
    20401 cgacgatctc ggcggtgccg gcactgacca gttcgccgta acgcgccttg ctggccgcgc
    20461 gcgcctgcgc ggcgaacgcc agccgcgcct ccagcggctg cgacaggaat tgcgcctgcc
    20521 agcgcgggtc gcgggtctgc gcgcggaact gctggtaggc ggggtcatcg gtgcacagca
    20581 ggtcgccgtg gaggatcacg gtgggcgtgc cgtacaggtc gatgacgctc ggttcgtcga
    20641 gcagcgacat cccgcagcgc gccgcgtagc cagcaccgag caggaagtcg cgattgccgc
    20701 ggatgaagtg caccggcacg ccggacgcgg ccagtgcgtg caatttgtcg gcgacgaatg
    20761 cgccggcatc ggagggatcg tcgtcaccga cccaggcttc gaacaggtcg ccgaggatgt
    20821 acagcgcgtc cgcgtccgcc gcttcttcct cgatgaatcg gccgaacaac gcggtgatcg
    20881 ccgggcgttc cgggtccagg tgcaggtcgg cgatgaacag cgtggtcatg cgggcattgt
    20941 agggattcgc tccctccctt tcgcacagcc aaggggaagg ttgggaaggg gttgctttcc
    21001 tcgtggacaa cgggcgcccc ccctcccagc ctccccctct ccgctgcgcg aaaaagggga
    21061 ggggcggatt gccggctaaa atgcgcccat tccgttcgat gccgcgccca tgaccgtccg
    21121 cacccgcttc gcccccagtc ccaccggcta cctgcacatc ggcggcgcgc gca
//
LOCUS       DAOOCX010000035        21030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2191_length_21030_cov_8.849106, whole genome shotgun sequence.
ACCESSION   DAOOCX010000035 DAOOCX010000000
VERSION     DAOOCX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 21030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21030
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_21030_cov_8.849106"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PK399_05125"
     CDS             <1..239
                     /locus_tag="PK399_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017160292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW12535.1"
                     /translation="MKGVIMAFWYLTSTFGSLWVLLTNVAVRNETVTAHIATTGYSEN
                     AFLMFFFAVFAFVAALAFAAYARTYPMQDNYRAA"
     gene            243..863
                     /locus_tag="PK399_05130"
     CDS             243..863
                     /locus_tag="PK399_05130"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPW12536.1"
                     /translation="MQLSLTIVIVAITVLVSWRAFNDRALMERMILWPPAVERRKQYD
                     RLLGYGFVHADWMHLAFNMITLWSFGSAVEREFSAMITPVGYVLFYLSAIVVSILPTY
                     LAHRNDPHYRSLGASGGVSAVLFAFILFDPWSTLIIFPIPVPIPAFLFAILYVAYSIW
                     MDRRGRDNVNHSAHLWGAAYGVLFTVLLEPRILGHFTQSLLGSATP"
     gene            876..1148
                     /locus_tag="PK399_05135"
     CDS             876..1148
                     /locus_tag="PK399_05135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW12537.1"
                     /translation="MTAIAHDPARQRFTTEADGQAAYLEYERADGLMTITHTIVPPDI
                     GGRGIAGDLVRTALEYARAEGVKVTPRCSYADGWMRKHPEYDALRA"
     gene            1180..1896
                     /locus_tag="PK399_05140"
     CDS             1180..1896
                     /locus_tag="PK399_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016849880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW12538.1"
                     /translation="MRLNKYIADTGFCSRREADRLVGEGRVTVNGIRAGIGAEVGEGD
                     KVQVDGNPLVARTAAKGQRRHVYIALNKPVGITSTTESGVKDNIVDFVDHAQRIFPVG
                     RLDKDSEGLILLTSNGDIVNRILRAENKLEKEYLVAVSRPVTMEFLRAMARGGVPIHG
                     EATLPCRTGKLGSQGFRIVLTQGLNRQIRLMAAHFGYRVKQLLRVRIGNVKLGHLKPG
                     QWRNLTDAELRGLLPDQVDW"
     gene            2142..2351
                     /locus_tag="PK399_05145"
     CDS             2142..2351
                     /locus_tag="PK399_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPW12539.1"
                     /translation="MSDRQTGTVKWFNDAKGFGFITPESGPDVFVHFRSIQGTGFKSL
                     QEGQKVTFKVVQGQKGLQADEVQAS"
     gene            2503..3330
                     /locus_tag="PK399_05150"
     CDS             2503..3330
                     /locus_tag="PK399_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12540.1"
                     /translation="MIRRTPTLVLASACALVLLSGCGREGNAPADVAAGPEAGQPAAA
                     AAPMALQDVIETRPEYIVGISYPQGIGKHPILARALQAYAESARADLMQAVAGLQGRK
                     PTAPYDLSLQFTGLVDTPRIVAAAADGSSYTGGAHGNPLVQRFVWLPQRNEMLGSQQL
                     IVDAAGWKVVSDYCREQLMTGLSQQLDDDGLEGEERSQALRTRSRMIDDGTAPTAGNF
                     ARFEPVMNADGSIRAVRFVFPPAQVGPYSDGTRSVDVPAQVLVPLVAPDYKPLFRAG"
     gene            3458..>7623
                     /locus_tag="PK399_05155"
     CDS             3458..>7623
                     /locus_tag="PK399_05155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW12541.1"
                     /translation="MPARPPPASVLDAFHPAVADWFARRFPAPTDAQQQAWPAIASGR
                     DTLVAAPTGSGKTLTAFLAAIDALVREALAHGLPDTTLVLYVSPLKALSNDIHLNLEA
                     PLAGIREALAAQALPDVDIRTAVRTGDTPQAERAALRKRPPHILVTTPESLYVLLGSE
                     SGRAMLGGVRTVIVDEIHALLPGKRGSHLALSLERLDALVGGRATRVGLSATQKPIED
                     AARFLVGTARVDATGRPDCRIVDIGHDKPRDLALELPLSPLGAVASGEQWEQVHDRLQ
                     ALAAEHRTTLVFVNTRRMAERAARHLSERLGKEHVAAHHGSMSKELRLDAEQRLKRGE
                     LKILVATASLELGIDIGDVDLVCQLGSPRSIAAFLQRVGRASHHVGGVPKGRLFATTR
                     DDLMECAALLDGVRRGELDRMPMPEAPLDVLAQVIVAEVASRDWDEDALFAMLVRAWP
                     YRALTREAFDGVLRMLAEGYASRFGTRGAWVHRDGVNRQLRARRGARQVAIQSGGTIP
                     DVGDYSVLLEPEGIGIGTVNEDFAIESLAGDIFQLGNASYRILRIEPGRLRVEDAQGA
                     PPSIPFWLGEAPGRTDVLSQAVSRLREELQAKLAEGEGIAQRWLVDDVGLSQEAARQL
                     LDYCVEQVAGFGLMPTQRQLAMERFFDEAGSTHLVIHAPFGSRINKAWGLALRKRFCR
                     SFNFELQAAATENAIVLSLSTSHSFPLLDVASFLHSNTARDILIQALLDAPLFPTRFR
                     WNASTALALPRFSGGKKVAPQLQRMKSEDLMATVFPDQVACLENIVGDRQVPDHPLVQ
                     QALTDCLCEAMDAEGWLEVLRGLESGRIDMVARDLTAPSVFAAEALNAAPYAFLDDAP
                     LEERRTQAVQSRRFGDGASVRDLGLLDASAIAGVREDAWPDVRNPDEMHEALMQLGAI
                     SAVEATASQWTPLLEALAAGRRATCLQVGDGLWTAAERLPQLQAVHPAAAPTPCIDVP
                     PGAQVPTGREEALRELVRGRLSASGPVRAAEIAAVLQVEANDIECALAALEVEGTAMR
                     GSFDPTLVGEQWCERHLLARIHRNTLGRLRREIEPVSLADAMRFLCDWQHLSARSRLR
                     GPDALASVLAQLEGWECAASSWEADVLPARLADYGIDWLDAQCSAGRIAWARLRMAAG
                     ADTGKGGRAPVRATPLVLVPRRELPRWAAIAGTTASGDGISSRAQLVVEALRAHGASF
                     FDELLDACRLLHVELEEALAELVARGIVHCDSYAGLRALLLPQGKRPNPGGRRMRRGA
                     ISGIRDAGRWSIARSAAGPPDADAVEYVAQVLLRRYGVVAWRLLEREAGWLPPWRELA
                     RVYRRMEARGEIRGGRFVAGIAGEQFALPEAVAGLRETRSRPKDGELIVVAASDPLNL
                     VGTLLPGDKLP"
     assembly_gap    7624..7723
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7724..7965
                     /locus_tag="PK399_05160"
     CDS             <7724..7965
                     /locus_tag="PK399_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009590870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12542.1"
                     /translation="ASDPLNLVGTLLPGDKLPRQSGARLVLRDGVPVAHKIGTTVEML
                     AALSPVDADAARQRLLRDPRLLGHVQVTTQAPVDG"
     gene            complement(7968..8609)
                     /gene="pdeM"
                     /locus_tag="PK399_05165"
     CDS             complement(7968..8609)
                     /gene="pdeM"
                     /locus_tag="PK399_05165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HPW12543.1"
                     /translation="MPESLDIVLAGEPLRLLADRALHWPARRRLFIADLHLGKADTFR
                     AAGIALPRGGTAFDLGRISCLVDATDADSLWVLGDMLHGRTDLSSWRTAWEDFRNRHP
                     RLSIAVVDGNHDRALQQAGLDVERLGDAVHDGPFVLRHAPARDPRGHVLCGHLHPVLK
                     LPGQARTPAFWLQPGCTILPAFSAFTGGHPLRLARGETAVLCNGAALVRVGER"
     gene            complement(8602..11076)
                     /locus_tag="PK399_05170"
     CDS             complement(8602..11076)
                     /locus_tag="PK399_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HPW12544.1"
                     /translation="MKATVKPRATFDAPLAAWFARQGWTPAPFQRAAWRHYLAGRDGL
                     LVTPTGSGKTLAALGGPLLQALRDPPPPKRKRGEIVAARTRVLWITPLRALANDTTRA
                     LREPIEALGLPWTVAMRTGDASARDKRLARNGQSDVLVITPESLALLLSYPETAGQLR
                     GLRAVIVDEWHELLGSKRGVLLQLCLARLRALSPAMRNWGLSATLGNLGEARDALLPH
                     APDAPIIDAAKPRSLTLETLLPEAGERFAWAGHLGLSQLQRVVARLQGVRSTLLFANT
                     RSQAELWHKALQSVWLEDPTMLALHHGSIDPKLRQAAEQGLRDGSVRCVVATSSLDLG
                     VDFPAVDQVIQIGSPKGMARLLQRAGRARHRPGEAGHILCVPTHALELVEYAAARKAL
                     RAGRVEARPPPRLSLDVLAQHCVTLALGGGFDADALFAEVRATHAFTELDAGTWQAVL
                     DFIVQGGKALAHYPDYHRVVRDEDGMYRVVDRKLAFRHRLSIGTITSDGHVSVQFAKG
                     GRLGSVEESFIGRLRPRDRFQFAGKTLELVQLKDMTAYVRLAKRNDGIVPRWQGSRMP
                     LSSMLAEAVQATLAGPRDTPELRAVSPMLDTQARLSALPTPDTLLAEQLATREGWHLF
                     LYPFAGRAVHEGLAALLALRWGRLHPNTFAFAVNDYGLAITAQARHLLDDDALRALLQ
                     EGALFDDLQASLNLAELARRQFRDIARVSGLLSPALPGRAPRSMRQLQASSGLLFDVL
                     SRFDADHLLLEQARREVFEAQLEVQALRATLADCARRTLSMQHPDSLTPLSFPLWAEA
                     MRGSLSTEDWSTRMRRAAEALERTHA"
     gene            complement(11073..12674)
                     /locus_tag="PK399_05175"
     CDS             complement(11073..12674)
                     /locus_tag="PK399_05175"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HPW12545.1"
                     /translation="MRRFTELFTRLDRSTGTGDKRAALVDYFRDAPPEDAAWALWLLS
                     GEKIGGAKARIAGSAELRAWVAEESGTPAWLVDDSYDAVGDLAETLALLLDDPHEIAD
                     DAPLHAWIEHRVLPVANADAEIRRAMVVQAWRTLPFAQRFVFNKLLTGALRVGVSRGL
                     VQQAIAEASGVDIALIAQRMLGAWSPTPEFLQRLTSREAQPGDAASPYPFFLASPLEA
                     EAKTLGAIDDWLLEWKWDGIRLQLIRRSGDIALWSRGEERLDGRFPEIEAALATLPRD
                     AVVDGELLAWRDGEQPMPFTALQTRIQRRKPGPKTLADTPVRVLAYDLLELDGEDLRA
                     RLLAERRALLEELLEAHGDPRIAVSPRIEAASWRDAAALREGSRARGVEGLMLKRLAA
                     PYQHGRKRGDWWKWKIDPLTIDAVLIYAQAGHGRRSTLYTDYTFAVWDGDALVPVAKA
                     YSGLDDKDILKLDSWIRGHTTERFGPVRAVKPHHVFELGFEAVNRSARHKSGIAVRFP
                     RILRWRQDKPMQDADRLDTLQALAQ"
     gene            complement(12674..13663)
                     /locus_tag="PK399_05180"
     CDS             complement(12674..13663)
                     /locus_tag="PK399_05180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HPW12546.1"
                     /translation="MPDLVVLRPEGLYCPAGDFHIDPWRPVPRAVITHGHGDHARVGM
                     GEYHAAAPGLPILQWRLGEQVYYPHEYGEVFTLGNARVSLHSAGHVLGSAQVRIEVDD
                     EAWVASGDYKRQHDPTCAPFEVVPCDVFITEATFGLPIYRWPDTGEVARDIVAWRDEC
                     AARGETAILYCYALGKAQRLLAELAAHTDRPAFLHGAIDTGTAIYREAGIPMLETRRI
                     ADEPRGSDFAGELVLAPPSAAGSPWIRRFRHAQQGFASGWMRIRGNRRRRNYDRGFVV
                     SDHADWPALLQTVRETGARRIIATHGATDALVRALNEHGIETGVFKTDFGGED"
     gene            13767..15029
                     /locus_tag="PK399_05185"
     CDS             13767..15029
                     /locus_tag="PK399_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HPW12547.1"
                     /translation="METIKKLAILADAAKYDASCASSGANRRNSSKTGGIGSTEGMGI
                     CHSYTPDGRCVSLLKILLTNFCVFDCAYCVNRVSSNVPRARFATDEVVRLTLDFYKRN
                     YIEGLFLSSGIIRSGDYTMEQLVEVARSLREDHRFAGYIHLKTIPDASPELLAAAGRY
                     ADRLSINVELPTEAGLKQLAPEKSQAGIRSAMGQLRWRIEEAKEARKPANAGFDRPTR
                     KARPPRFAPAGQSTQMIVGADGANDRDVLQASDRLYGDYRMRRVYYSAFSPIPDASKA
                     LPLIAPPLQREHRLYQADWLLRFYEFKVEEIAPPSANGMLDLDIDPKLAWALRHPQAF
                     PVDLNTAPKEALLRVPGLGVRNVKRVLMARRHTRLRLADIARLRAPMTKLMPFIATSD
                     HHPRQRLDDPAALRAQLAPKPHQADLFG"
     gene            15043..16464
                     /locus_tag="PK399_05190"
     CDS             15043..16464
                     /locus_tag="PK399_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660951.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HPW12548.1"
                     /translation="MWSAAVDPAWSVDAFREAARAALRAGIAPEGLEWLADGQRGLLA
                     LPDVLDAPVLHASPRVLRSFLDIAETVLCHRDPQRHALLYRLLWRIAHGEPHVLATPT
                     DADTRRVMQLAQEVRRDAHKMKAFVRFREVPGERDNFIAWFEPSHHILHRVAPFFARR
                     FAGMRWAILTPEGSALWDGEALSFGPGGAPDDAPAFDAGENLWRTYYAHIFNPARLNP
                     RMMRQEMPQRYWKHLPEAHLLPSLIQGAAVRVREMAEREHAAPKRRIPAPTVVPPTDD
                     TRRDESLASLRQAAQACRACPLWQPATQTVFGEGPEDARVLVLGEQPGDREDLSGRPF
                     VGPAGKLLDRALAELGIDRSQLYLTNAVKHFRFERRGKQRLHRNPEASHVQACRSWLA
                     GEIERVRPQMIVCLGGTAALAVFGAGFRLTAQRGTWQTLHDGTRAFATVHPAWILRQP
                     ADRQAGAYEAWLDDLRLLLATAG"
     gene            16564..17604
                     /locus_tag="PK399_05195"
     CDS             16564..17604
                     /locus_tag="PK399_05195"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW12549.1"
                     /translation="MEFRSILLLALALCAAIPAEAQVAKRQPIIDMHLHVLAPDAQGP
                     PPLGMCTPIAEMPAWDPGAGPYVDTIMAIFKKPSCKDPVWSPDTREEMRARTLAELEK
                     HDVIGVLSGDSDKDIADWRKASPERIIPALGDSIRMPVVDAATVARVKAMKASGDLVV
                     LSEIGPQYMGMAPDDPRLEPFWALAEELDLPVGIHVGTGPPGTPYLAPPLKDYRARLH
                     SALVIEEVLARHPKLRVYLMHAGYPMIDDLLAVLYTHPQVYVDVGVIVYTQPRPAFYR
                     YLQAIVEAGFAKRVMFGSDNMVWPETIGRSIEVIQQAPFLSEAQKRDIFYNNAARFLR
                     LDAATIARHHAL"
     gene            complement(17662..19830)
                     /locus_tag="PK399_05200"
     CDS             complement(17662..19830)
                     /locus_tag="PK399_05200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPW12550.1"
                     /translation="MKHPISCALALAIATALAGCATTAPATNTSEATMPATYSGPFAA
                     PSTLDLHYPQFDAIKDSDFAPAFDAGMAQQLREIQAIANNPEAPSFQNTIVAMEKSGQ
                     VLDRATNVFFNLIGTDKNDAREKLESDYAPKFSAHRDAIALDPKLFARIKTLHDARAT
                     LGLDAIDLRLLEKRYTDFVRSGAALDDAQKARIREINTDLSKLGTQFNQNVLAEVNDS
                     AIVVDSRDELKGMGEEQIAAAAEAAKARGLEGKYVIALLNTTGQPAEAQLENRALRER
                     LHKASVARGSRGNQWDNTGIVSQVLKLRAERATLLGYDTYANYVLADETAANQDNANR
                     LLRQLAPAAVNNARREGADLQAMIDAEQEAKRQPTFQLAPWDWSYYSEKVRAQKYSFD
                     ESQLKPYFEMKRVLEDGVFFAATKLYGITFKERTDLPKYHPDTWIYDVYDKDGSLLSI
                     FIWDPYARSSKRGGAWMNTYVAQSDLTGDKPVVANHLNIPKPSAGKPTLMTWDEVTTA
                     FHEFGHALHGFFQDVRYPYYSMNVPRDFVEYPSQVNEMWADWPSVLGNYAKHYQTGEP
                     MPKALLDKVIAAAKFNQGFATTEYLEAAMLDQRWHQLPAAQIPAASGVMAFEAQALKA
                     DGFDYAPVPPRYRTPYFSHIMGGYAAGYYAYIWSEVLGANTEQWIRNNGGLTLQNGDR
                     LRKFVLAPGGEIEAGKLFPNFSGYEAKIEPLLEKRGLTAK"
     gene            19972..20400
                     /locus_tag="PK399_05205"
     CDS             19972..20400
                     /locus_tag="PK399_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW12551.1"
                     /translation="MAQVTSVMTASPSCCREDTPLRDVAQLMIDHDCGMIPVVDADGR
                     PVGAVTDRDIAVRIVARGKDASSSRAGDAMTSPVTTIDSATSLHDATCVMEAEKIRRV
                     IVVDADGKLAGVAAIADLALAGKNEATAAVVREISEPGRA"
     gene            20478..20963
                     /locus_tag="PK399_05210"
     CDS             20478..20963
                     /locus_tag="PK399_05210"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPW12552.1"
                     /translation="MATTAYDFDATALDGTPQPLSDWRGKVLLIVNVASKCGFTPQYA
                     GLEKLWRDYGDKGLVVLGFPCDQFGHQEPGNAEEIRNFCSLTYDVSFPMFAKIDVNGA
                     HAHPLWTWLKDEKGGLLGFDGIKWNFTKFLVGRDGKVIKRYAPTDKPESLARDIEKAL
                     G"
BASE COUNT         3233 a         7107 c         7283 g         3307 t
ORIGIN      
        1 cgatgaaggg cgtgatcatg gcgttctggt acctcaccag taccttcggc agcctgtggg
       61 tgctgctcac caacgtcgcc gtgcgcaacg aaacggtgac cgcgcacatc gccaccaccg
      121 ggtacagcga gaacgcgttc ctgatgttct tcttcgcggt cttcgccttc gtcgccgcgc
      181 ttgcattcgc cgcgtatgcg cggacgtacc cgatgcaaga caattaccgg gcggcctgag
      241 ccatgcagtt gtcgctgacc atcgtcatcg tcgccatcac cgtgctggtg tcctggcgcg
      301 cattcaacga ccgcgcgctg atggagcgga tgatcctgtg gccgccggcg gtggagcggc
      361 gcaagcagta cgaccgcctg ctgggctacg gcttcgtgca tgccgactgg atgcacctcg
      421 ccttcaacat gatcaccctg tggtcgttcg gcagcgcggt ggagcgcgag ttctcggcga
      481 tgatcacgcc ggtcggctac gtcctgttct acctgtccgc gatcgtggtc tcgatcctcc
      541 cgacctacct tgcccatcga aacgatccgc actaccgcag cctgggcgcc tccggcggcg
      601 tgtcggcggt gctgttcgcc ttcatcctgt tcgacccgtg gtcgaccctg atcatcttcc
      661 cgatcccggt accgatcccc gccttcctgt tcgcgatcct ctatgtcgcc tacagcatct
      721 ggatggatcg ccgcggccgc gacaacgtca accacagtgc ccacctctgg ggcgccgcct
      781 acggcgtgct gttcaccgtg ctgctggaac cgcggattct cgggcacttc acgcagtccc
      841 tgctgggatc ggccactcct tgaacggaaa cagccatgac tgccattgcc catgatcccg
      901 cgcgccagcg cttcaccacc gaggcagatg gccaggccgc ctacctcgaa tacgaacggg
      961 cggatggcct gatgaccatc acccacacca tcgtgccgcc ggacatcggg gggcgtggca
     1021 tcgccggcga cctggtgcgt accgcgcttg agtacgccag ggccgaaggc gtgaaggtca
     1081 cgccgcgctg ctcctacgcg gatggctgga tgcgcaagca ccccgaatac gacgcactgc
     1141 gcgcctgagc cgcgggtgcg gcgggtagaa tccccgccca tgcgcctcaa caaatacatc
     1201 gccgacaccg gtttctgctc ccgccgcgag gccgaccgcc tggttggcga aggccgggtc
     1261 acggtcaatg ggatccgcgc cgggatcggc gccgaggtgg gcgagggcga caaggtccag
     1321 gtcgacggca acccgctggt ggcgcgcacc gccgccaagg gccagcgccg gcacgtttat
     1381 atcgcgctga acaagccggt cgggatcacc agcaccaccg aatccggggt caaggacaac
     1441 atcgtcgatt tcgtcgacca cgcgcagcgg atcttcccgg tcggccggct ggacaaggat
     1501 tccgaagggc tgatcctgtt gaccagcaat ggcgacatcg tcaaccggat cctgcgcgcg
     1561 gagaacaagc tggaaaagga atacctggtc gcggtcagcc ggccggtcac catggaattc
     1621 ctgcgggcga tggcgcgggg cggggtgccg atccacggcg aggccacgtt gccctgcagg
     1681 accggcaagc tgggcagcca gggcttccgg atcgtcctga cccagggcct gaaccggcag
     1741 atccgcctga tggcggcgca tttcggctac cgggtgaagc agctgctgcg ggtgcgcatc
     1801 ggcaacgtga agctgggcca cctcaagccc ggccaatggc gcaacctgac cgacgccgaa
     1861 ttgcgcgggc tgctgcccga ccaggtcgac tggtagccgc ttgccggggg cgcccgggca
     1921 cccgcaaaaa aaatcccggg ccattcacat ttgtccccga aaacaggtta tggtgcgccc
     1981 actgtttcaa gcgggatcac ggtacatcgt gacccgatgc ggtagatccg agtgatccgc
     2041 tacatcgtgt gcgttacccc ttgctcgaca gtcgcggccc agccttccaa cgaatccggc
     2101 gggtcgtgtc accatttcaa actgttctag gagtagcaaa catgtcggat cgtcagactg
     2161 gtaccgtcaa gtggttcaac gacgccaagg gcttcggctt catcaccccg gaaagcggcc
     2221 cggacgtctt cgtccacttc cgctcgatcc agggcaccgg cttcaagtcg ctgcaggaag
     2281 gccagaaggt gaccttcaag gtcgtgcagg gccagaaggg cctgcaggcc gacgaagtgc
     2341 aggcctcgta agagacccgc gcatcgcgct caaaaaaccc ggcttcggcc gggttttttt
     2401 tatgcatgct gtttccgtat cacgctggcg acgtccacat caccgcgcat gaggcatcat
     2461 ggcgcccgcc cggtgacctc cctcgattcg tccagaccgc ccatgatccg ccgcacgccg
     2521 actctcgtac tcgcctccgc ctgtgccctt gtcctgctct ccgggtgtgg acgcgagggc
     2581 aacgcgcccg ccgacgttgc cgcgggtccg gaggcgggcc aacccgcggc cgcggccgcg
     2641 ccgatggccc tgcaggacgt gatcgaaacc aggccggagt acatcgtcgg catcagttat
     2701 ccgcagggga tcggcaagca tccgatcctc gcccgggccc tgcaggcgta cgccgagtcc
     2761 gcccgcgccg acctgatgca ggccgtcgcg gggctgcagg gccgcaagcc gacggcaccg
     2821 tacgatctca gcctgcagtt caccggcctg gtggacacgc ctcggatcgt cgcggcggcg
     2881 gcggacggca gcagctacac cggcggcgcg cacggcaacc cgctggtgca gcgcttcgtc
     2941 tggttgcccc agcgcaacga gatgctgggc tcgcaacagc tgatcgtcga tgcggcgggc
     3001 tggaaggtgg tgtccgacta ttgccgcgag cagctcatga ccgggctctc gcagcaactc
     3061 gatgacgacg gactggaagg cgaggagcgg tcgcaggcgc tgcgcacccg cagcaggatg
     3121 atcgatgacg ggaccgcgcc gacggcaggc aacttcgccc gcttcgagcc ggtgatgaat
     3181 gcggacggca gcatccgcgc cgtgcgcttc gtgttcccgc ccgcgcaggt cggcccttat
     3241 tccgatggca cccgtagcgt ggacgtcccg gcgcaggtgc tggtgccgct ggtggcgccg
     3301 gactacaagc ccttgttccg cgccggctga cccggtctca ttggctgaga cggcacccgc
     3361 gcaggctgcg cgggtgaacg cgcagatccg cttcgatttc agcccgacgg cccgcaccgt
     3421 gcgtggcggc catggcgatg gggcggataa tcctggcatg cccgcgcgcc cccccccagc
     3481 ctcggtgctc gacgccttcc atccggccgt cgccgactgg ttcgcgcgcc gcttccccgc
     3541 gcccaccgac gcccaacagc aggcctggcc ggcgatcgca tccggccgcg acaccctggt
     3601 ggcggcgccg accggctccg gcaagacgct cacggccttc ctcgccgcga tcgacgcgct
     3661 ggtgcgcgaa gcgctggcgc acgggctgcc ggacaccacc ctggtgctgt acgtctcgcc
     3721 gctgaaggcg ctgtcgaacg acatccatct gaacctggaa gcgccgctgg ccggcatccg
     3781 cgaggcactg gccgcgcagg ccttgcccga cgtcgacatc cgcaccgcgg tgcgcaccgg
     3841 cgacaccccg caggccgaac gcgccgcatt gcgcaagcgg ccgccgcaca tcctggtgac
     3901 cacgccggaa tcgttgtacg tgctgctggg ctccgagtcc ggccgggcga tgctgggtgg
     3961 cgtgcgcacg gtgatcgtcg atgaaatcca tgcgctgctg ccgggcaagc gcggcagcca
     4021 cctggccttg tcgctggaac gactggatgc gctggtcggt gggcgggcga cgcgcgtggg
     4081 cctgtcggcc acgcagaagc cgatcgagga cgcggcgcgt ttcctggtgg ggaccgcgcg
     4141 ggtggacgcg accggccgac ccgattgccg catcgtcgac atcggccacg acaagccgcg
     4201 cgacctcgcg ctcgaactgc cgttgtcgcc cctgggcgcg gtcgccagcg gcgagcagtg
     4261 ggagcaggtg cacgaccgcc tgcaggcact ggcggcggaa caccggacca cgctcgtctt
     4321 cgtcaacacc cggcgcatgg ccgaacgcgc ggcgcgccac ctgagcgagc ggctgggcaa
     4381 ggagcacgtg gccgcgcacc acgggtcgat gtcgaaggag ctgcggctgg atgccgagca
     4441 gcgcctgaag cgcggcgaat tgaagatcct ggtggccacc gcgtcgctgg aactcggcat
     4501 cgacatcggc gatgtcgacc tggtctgcca gctcggcagc ccgcgcagca tcgccgcgtt
     4561 cctgcagcgc gtgggccgcg ccagccacca cgtcggcggc gtgccgaaag ggcgcctgtt
     4621 cgcgaccacc cgcgacgacc tgatggaatg cgccgcgctg ctggatggcg tgcgtcgcgg
     4681 cgagctggat cgcatgccga tgccggaagc gccgctggac gtgctggcgc aggtgatcgt
     4741 ggctgaagtg gccagtcgcg attgggacga agatgcgttg ttcgcgatgc tggttcgcgc
     4801 atggccctac cgcgcgctca cccgcgaggc attcgatggc gtgctcagga tgctggccga
     4861 aggctatgcc agccgcttcg gcacccgcgg cgcgtgggtg catcgcgacg gcgtgaaccg
     4921 gcagttgcgt gcgcgccgcg gcgcgcgcca ggtggcgatc cagagcggcg gcacgattcc
     4981 cgatgtcggc gattactcgg tgctgctgga gccggagggc atcggtatcg gcacggtcaa
     5041 cgaagacttc gcgatcgaga gcctggccgg cgacatcttc cagttgggca atgcgagcta
     5101 ccgcatcctc cgtatcgagc cggggcgcct gcgggtggag gatgcgcagg gcgcgccgcc
     5161 gtcgatcccg ttctggctgg gcgaggcgcc gggccgcacc gacgtgctgt cgcaggcggt
     5221 ctcgcgcctg cgcgaggaac tgcaggcgaa actcgcggaa ggcgagggca tcgcccagcg
     5281 ctggctggtg gacgatgtcg gtctttcgca ggaagcggcg cggcagttgc tggactactg
     5341 cgtcgagcag gtcgccggct tcgggctgat gccgacgcag cgccagctgg cgatggagcg
     5401 cttcttcgac gaggccggtt ccacccacct ggtgatccac gcgcccttcg gcagccgcat
     5461 caacaaggcc tggggcctgg ccctgcgcaa gcgcttctgc cgcagcttca acttcgagtt
     5521 gcaggcggcc gccacggaaa acgcgatcgt gctgtcgctg tcgaccagcc acagcttccc
     5581 gttgctcgac gtcgcctcgt tcctgcattc gaataccgcc cgcgacatcc tgatccaggc
     5641 gctgctggat gcgccgctgt tcccgacgcg cttccgctgg aacgcgagca ccgcgctggc
     5701 gctgccgcgc ttcagtggcg gcaagaaggt ggcgccgcaa ttgcagcgga tgaagtccga
     5761 agacctgatg gccacggtgt tcccggacca ggtcgcctgc ctggagaaca tcgtcggcga
     5821 ccgccaggtg cccgaccatc cgctggtcca gcaggcgctc accgattgcc tgtgcgaggc
     5881 gatggatgcc gaaggctggc tggaggtgct gcgcgggctg gagagcggcc gcatcgacat
     5941 ggtcgcccgt gacctcaccg cgcccagcgt gttcgcggca gaggcgctga atgccgcgcc
     6001 gtatgcgttc ctcgacgatg cgccgctgga ggaacgccgc acccaggcgg tgcaatcgag
     6061 acgcttcggc gatggtgcgt cggtgcgcga cctcggcctg ctcgacgcca gcgcgatcgc
     6121 cggcgtgcgc gaggacgcct ggcccgacgt gcgcaacccc gacgagatgc acgaggccct
     6181 gatgcaactc ggcgcgatca gcgccgtcga agccaccgca tcgcagtgga cgccgttgct
     6241 ggaggcgttg gcggccggtc gccgcgcgac ctgcttgcag gtcggcgatg ggctatggac
     6301 cgccgccgag cgcctgccgc aattgcaggc ggtgcatccg gccgccgcgc ccacgccctg
     6361 catcgatgtg ccgcccggtg cgcaggtccc cacgggccgc gaagaggcgt tgcgtgagct
     6421 cgtgcgcggt cgcctgagtg ccagtggccc ggtgcgggcg gcggagatcg ccgccgtgct
     6481 tcaggtcgag gcgaacgaca tcgaatgcgc gctggccgcg ctggaagtgg aaggcaccgc
     6541 gatgcgcggc agcttcgatc ccaccctggt tggcgagcag tggtgcgagc gccacctgct
     6601 ggcgcgcatc catcgcaata cgctgggccg gttacggcgc gagatcgaac cggtgtcgct
     6661 ggccgatgcg atgcgcttcc tgtgcgactg gcagcacctg tccgcgcgca gccgcctgcg
     6721 cgggccggat gcgctggcca gcgtgctggc gcaactggaa ggctgggagt gcgcggccag
     6781 cagttgggag gcggacgtgt tgcctgcgcg gctggccgat tacgggatcg actggctgga
     6841 cgcacagtgc agcgccgggc gcatcgcatg ggcgcggctg cgcatggccg cgggcgcgga
     6901 caccggcaag ggcggtcgcg cgccggtgcg ggccacgccg ctggtgctgg tgccgcgtcg
     6961 cgagttgccg cgctgggcgg cgattgccgg caccaccgcc agcggcgacg ggatctcctc
     7021 gcgcgcacag ttggtggtgg aggccttgcg cgcgcatggc gcgtcgttct tcgacgaatt
     7081 gctggatgcg tgccgcctgc tgcacgtcga gctggaagag gcgctggccg agctggtggc
     7141 gcgcggcatc gtccattgcg acagttacgc cggcctgcgc gcattgttgc tgccgcaggg
     7201 caagcggccc aatcccggtg gccggcgcat gcgtcgcggc gcgatttccg ggatccgcga
     7261 tgccggccgc tggagcatcg cgcgcagcgc ggcgggtccg cctgatgccg acgccgtcga
     7321 gtacgtcgcg caggtgctgt tgcgtcgcta cggcgtggtg gcgtggcggc tgctcgagcg
     7381 cgaagccggc tggctgccgc cgtggcggga actcgcgcgg gtgtaccggc gcatggaagc
     7441 gcgcggcgag atacgcggcg ggcgcttcgt ggccgggatc gccggcgagc agttcgcctt
     7501 gccggaagcg gtggcgggcc tgcgcgaaac ccgcagtcgg ccgaaggacg gcgaactcat
     7561 cgtggtcgcc gccagcgatc cgctaaacct ggtcggcacg ctgctgccgg gcgacaagtt
     7621 gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgccag cgatccgcta
     7741 aacctggtcg gcacgctgct gccgggcgac aagttgccgc gccagtcggg tgcccggttg
     7801 gtcctccgcg atggcgtgcc ggtggcgcac aagatcggca ccacggtcga gatgctggca
     7861 gcactttcgc cggtcgatgc cgatgcggca cgccaacgcc tgctgcgcga tccgcgcctg
     7921 ctcgggcatg tgcaggtgac gacgcaggcg ccggtcgacg gctgacctca gcgctcgccc
     7981 acgcgcacca gcgccgcgcc gttgcagagc acggcggtct ctccgcgggc gagccgcaac
     8041 ggatggccac cagtgaaggc cgagaacgcc ggcaggatcg tgcagccggg ttgcaaccag
     8101 aacgcgggcg tgcgcgcctg cccaggcagc ttcagcaccg gatgcaggtg cccgcacagc
     8161 acatggccgc gcgggtcgcg cgcgggggca tggcgcagca cgaacggccc atcgtgcacg
     8221 gcatcgccca gtcgttcgac gtccagtccc gcttgctgca gcgcgcggtc gtggttgccg
     8281 tcgaccactg cgatcgacag gcgcggatgc cggttgcgga agtcctccca tgcggtgcgc
     8341 cacgacgaca ggtcagtgcg cccgtgcagc atatcgccga gcacccacag cgaatcggca
     8401 tcggtggcgt cgaccaagca cgagatgcgg ccgaggtcga acgcggtgcc gccacgcggc
     8461 agggcgatgc cggccgcacg gaacgtatcg gctttgccga ggtgcaggtc cgcgatgaac
     8521 aggcgtcgcc gcgctggcca atgcagcgcg cggtcggcga gcaagcgcag cggttcgccg
     8581 gcaagcacga tgtccagcga ctcaggcatg cgtgcgttcc agtgcttcgg cggcgcgacg
     8641 catccgcgtg ctccagtcct cggtgctcag gctgccacgc atcgcctccg cccacagcgg
     8701 gaacgacagc ggggtcagcg agtcggggtg ttgcatcgac agcgtgcgtc tcgcgcaatc
     8761 ggccagcgtc gcgcgcagtg cctgcacctc gagttgcgct tcgaagacct cgcgacgcgc
     8821 ctgttccagc agcaggtggt cggcatcgaa gcgcgagagc acgtcgaaca gcagcccgct
     8881 ggacgcctgc aactggcgca tcgagcgcgg cgcgcgaccg ggcagggcgg gcgagagcag
     8941 gccggacacg cgcgcgatgt cgcggaactg ccggcgcgcc agttcggcca ggttgaggct
     9001 ggcctgcagg tcgtcgaaca acgcgccctc ctgcagcagc gcgcgcaggg cgtcgtcatc
     9061 cagcaggtgg cgcgcctgcg cggtgatcgc cagtccgtag tcgttgaccg cgaaggcgaa
     9121 ggtgttcgga tgcaggcggc cccagcgcag cgccagcagg gcggccaggc cttcgtgcac
     9181 ggcgcgcccg gcgaacggat acaggaacag gtgccagccc tcgcgcgtgg ccagttgctc
     9241 ggccagcagg gtgtccggcg tgggcagcgc ggacagtcgc gcctgggtgt cgagcatggg
     9301 cgacaccgcg cgcagttcgg gcgtgtcgcg cgggccggcg agggtggcct gcaccgcttc
     9361 ggccagcatg ctcgacagcg gcatgcggct gccctgccag cgcggcacga tgccgtcgtt
     9421 gcgcttggcc aggcgcacgt aggcggtcat gtccttcagc tggaccagct ccagggtctt
     9481 gccggcgaac tggaagcgat cgcgcgggcg caggcggccg atgaagctct cctcgaccga
     9541 gccgaggcgc ccgcccttgg cgaactgcac cgacacatgg ccgtcgctgg tgatggtgcc
     9601 gatcgacagg cgatggcgga atgccagctt gcggtcgacg acgcgataca tgccgtcctc
     9661 gtcgcggacc acgcggtggt agtcggggta atgcgccagc gccttgccgc cctgcacgat
     9721 gaagtcgagc acggcttgcc aggtgccggc gtccagctcg gtaaaggcat gcgtggcgcg
     9781 gacttcggca aacaacgcat cggcgtcgaa accaccgccc agggccaggg tcacgcaatg
     9841 ctgcgcgagc acgtcgagcg agagccgcgg cgggggccgc gcttcgaccc ggccggcccg
     9901 cagcgccttg cgcgcggcgg cgtattcgac cagctccagc gcatgggtcg gcacgcacag
     9961 gatgtggccg gcttcgcccg ggcgatggcg cgcacgtccg gcccgctgca acagccgcgc
    10021 catgcccttg gggctgccga tctggatgac ctggtccacc gccgggaagt ccacgccgag
    10081 gtcgaggctg gaggtcgcga ccacgcagcg cacgctgccg tcgcgcaggc cctgttcggc
    10141 ggcctggcgg agcttcgggt cgatggagcc gtggtgcagc gccagcatcg tcggatcctc
    10201 cagccacacc gactgcagcg ccttgtgcca caactccgcc tgcgagcgcg tattggcgaa
    10261 caacaaagtg ctgcgcacgc cctgcaggcg cgccaccacg cgttgcaact gcgagaggcc
    10321 gagatggccg gcccacgcga accgctcgcc ggcctccggc agcagggttt ccagggtcag
    10381 cgaccgcggc ttcgcggcgt cgatgatcgg tgcgtccggc gcatgcggca gcagggcgtc
    10441 gcgcgcttcc ccgaggttgc cgagcgtcgc cgacaagccc cagttgcgca tcgcgggaga
    10501 cagtgcccgc agacgcgcca ggcacagttg cagtaggacc ccgcgcttgc tgccgagcaa
    10561 ctcgtgccac tcgtcaacga tgacggcgcg caagccgcgc agctggcccg cggtctccgg
    10621 atacgacagc aacagcgcca aggattccgg ggtgatcacc agcacgtcgg attggccgtt
    10681 gcgcgccagt cgcttgtcgc gggcactggc atcgccggtg cgcatcgcga cggtccacgg
    10741 cagcccgagc gcttcgatcg gttcgcggag cgcacgcgtg gtgtcgttgg ccagcgcacg
    10801 caacggtgtg atccacagca cgcgggtgcg cgcggccacg atttcgccgc gcttgcgttt
    10861 cggcggaggc ggatcgcgca aggcctgcag cagcgggccg ccgagggccg ccagggtctt
    10921 gccgctgccg gtcggcgtga ccagcaatcc atcgcgaccg gccaggtagt gccgccacgc
    10981 ggcgcgctgg aacggcgcgg gcgtccagcc ctgtcttgcg aaccacgccg ccagcggcgc
    11041 atcgaatgtc gcgcgtggtt ttaccgtcgc cttcattgcg ccagtgcctg caaggtgtcg
    11101 aggcggtcgg cgtcctgcat cggcttgtcc tggcgccagc gcaatatccg cgggaagcgc
    11161 accgcgatcc ccgacttgtg gcgtgcggag cgattcaccg cctcgaagcc gagctcgaac
    11221 acatgatgcg gcttcaccgc acgcaccggg ccgaaacgct cggtggtgtg gccgcggatc
    11281 cagctgtcga gcttgaggat gtccttgtcg tccaggcccg aataggcctt ggccaccggc
    11341 accagcgcgt cgccgtccca gaccgcgaag gtgtagtcgg tgtacagcgt gctgcgccga
    11401 ccgtggccgg cctgcgcgta gatcagcacc gcatcgatgg tcagcgggtc gatcttccat
    11461 ttccaccagt cgccgcgctt gcggccgtgc tggtatggcg cggccaggcg cttgagcatc
    11521 aggccctcga cgccgcgtgc gcggctgcct tcgcgcagcg cggcggcatc ccgccatgac
    11581 gccgcctcga tgcgcggcga caccgcgatc cgcgggtcgc catgggcctc aagcaattcc
    11641 tccagcaatg cgcgccgctc ggcgagcagg cgcgcacgca ggtcttcgcc atccagttcc
    11701 agcaggtcgt aggcgagcac gcgcaccggc gtgtcggcca gcgtcttcgg cccgggcttg
    11761 cgccgctgga tgcgggtctg cagcgcggtg aacggcatgg gctgctcgcc gtcccgccag
    11821 gcgagcagtt cgccgtcgac cacggcatcg cgcggcagcg tggcgagtgc ggcttcgatt
    11881 tcggggaagc gtccgtcgag gcgctcctcg ccgcgcgacc acaacgcgat gtcgccgctg
    11941 cggcggatca gctgcaggcg gatgccatcc catttccatt ccagcagcca gtcatcgatc
    12001 gcacccagcg ttttggcctc ggcttccaat ggcgaggcca ggaagaacgg atagggtgat
    12061 gccgcatcgc cgggttgcgc ctcgcgcgag gtcaggcgct gcaggaactc cggcgtgggc
    12121 gaccacgcgc cgagcatgcg ctgcgcgatc agggcgatgt ccacgcccga cgcttccgca
    12181 atggcttgct gcacaagccc gcgggacacg ccgacccgta atgcgccggt cagcagtttg
    12241 ttgaacacga agcgctgcgc aaacggcagc gtgcgccaag cctggaccac cattgcccgg
    12301 cgaatctccg catcggcatt ggcgaccggc agcacgcggt gctcgatcca cgcgtgcaac
    12361 ggtgcgtcat cggcaatttc gtgcggatca tcgagcagca aggccagggt ttccgcgagg
    12421 tcgccgaccg cgtcgtagct gtcgtccacc agccaggcgg gcgtgccgct ttcctccgcg
    12481 acccaggcgc gcaactccgc gctgccggcg atccgcgcct tcgccccgcc gatcttttcg
    12541 ccggagagta accacaaggc ccacgcggcg tcttccggcg gcgcatcgcg gaagtaatcg
    12601 accagggcgg cgcgcttgtc gccggtgccg gtgctgcggt ccagacgggt gaacaactcg
    12661 gtgaagcggc gcatcagtct tcgccaccga aatcggtctt gaacacgccg gtctcgatgc
    12721 catgttcgtt cagcgcacgc accagcgcgt cggtcgcgcc gtgggtggcg atgatgcggc
    12781 gcgcgccggt ctcgcgcacg gtctgcaata gcgccggcca gtcggcgtgg tcggagacca
    12841 cgaagccacg gtcgtagttg cgccggcggc ggttgccgcg gatccgcatc cagccggagg
    12901 cgaagccctg ctgcgcatgg cggaagcggc ggatccatgg gctgcccgca gccgagggcg
    12961 gggcgagcac cagttcgccg gcgaagtcgc tgccgcgcgg ctcgtcggcg atgcggcgcg
    13021 tctccagcat cggaatgccg gcctcgcgat agatcgcggt gccggtatcg atggcgccat
    13081 gcaggaaggc ggggcggtcg gtgtgcgcgg ccagttcggc cagcaagcgc tgggccttgc
    13141 cgagggcgta gcagtacagg atcgcggttt cgccgcgcgc cgcgcactcg tcgcgccagg
    13201 ccacgatgtc gcgggccacc tcgccggtat ccggccagcg gtagatcggc aggccgaacg
    13261 tggcctcggt gatgaagacg tcgcagggca cgacctcgaa cggcgcgcag gtcgggtcgt
    13321 gctggcgctt gtaatcgccc gaggccaccc aggcctcgtc atcgacctcg atccgcacct
    13381 gcgcgctgcc cagcacgtgg ccagcagaat gcagcgagac ccgcgcattg ccgagggtga
    13441 atacctcgcc gtattcgtgc gggtaataga cctgttcgcc aagccgccat tgcaggatcg
    13501 gtagccccgg cgcggccgcg tggtactcgc ccatgccgac ccgcgcatgg tcgccgtggc
    13561 catgggtgat gaccgcgcgc ggcaccggcc gccacggatc gatatggaaa tcgcccgccg
    13621 ggcaatacag gccttcgggg cgcaggacga cgaggtctgg catcgggcaa agatgggggc
    13681 ggacgtgtgc aggccaagcg atgaatgatt cagcttgctg accacccatc gcagggattg
    13741 cgacccgatt ccggcagact ttcgcggtgg aaaccatcaa gaaactcgcc atcctggccg
    13801 acgccgccaa gtacgacgcc tcctgcgcgt cgagtggcgc gaacaggcgc aattccagca
    13861 agaccggcgg catcggcagc accgaaggga tgggcatctg ccacagctac acgccggatg
    13921 gccggtgcgt gtcgctgctc aagatcctgc tgacgaattt ctgcgtgttc gactgcgcct
    13981 attgcgtcaa ccgcgtgtcc agcaacgtgc cgcgtgcgcg cttcgccacc gacgaagtgg
    14041 tgcgcctcac cctcgacttc tacaagcgca actacatcga aggcctgttc ctctccagcg
    14101 gcatcatccg cagcggcgac tacaccatgg agcaactggt cgaggtcgcg cgcagcctgc
    14161 gcgaggacca ccgtttcgcc ggctacatcc acctcaagac gattcccgat gcgtcgcccg
    14221 agctgctggc cgcggcggga cgctatgcgg atcggctttc gatcaacgtc gaactgccca
    14281 ccgaggctgg cctgaagcag ttggcgccgg agaaatcgca ggccggcatc cgcagcgcga
    14341 tgggacagtt gcgctggcgc atcgaggaag cgaaggaggc gcgcaagccg gccaacgccg
    14401 gcttcgacag gcccacgcgc aaggccaggc ctccgcgatt cgcgccagcc gggcagagca
    14461 cgcagatgat cgtgggcgcg gacggtgcca acgaccgcga tgtcctgcag gcgtccgacc
    14521 gcctgtacgg cgactaccgc atgcgccgcg tgtattactc cgccttcagc ccgatcccgg
    14581 atgcgtcgaa ggcgttgccg ctcatcgcgc cgccgctgca gcgcgagcat cggctctacc
    14641 aggccgactg gttgctgcgc ttctacgaat tcaaggtcga ggaaatcgcg ccgccctcgg
    14701 ccaacgggat gctggacctc gacatcgatc ccaagctggc ctgggccttg cgccatccgc
    14761 aggcgttccc ggtcgacctc aataccgcgc cgaaggaagc gttgctgcgc gtcccggggc
    14821 tgggtgtgcg caacgtcaag cgcgtgctga tggcgcgccg gcatacacga ctgcgcttgg
    14881 ccgacatcgc ccgcttgcgc gcgccgatga ccaagctgat gccgttcatc gccaccagcg
    14941 accatcaccc gcggcaacgg ctggacgatc ccgccgcctt gcgggcgcaa ctcgcgccga
    15001 agccgcacca ggccgatctg ttcggatgag gcagtgccga tgatgtggtc ggcggcggtg
    15061 gatcccgcgt ggtcggtcga tgcgtttcgc gaggccgcgc gcgccgcgtt gcgcgccggc
    15121 atcgcacccg agggtcttga gtggcttgcc gatggccagc gtggcctgct ggcgctgccc
    15181 gacgtgctcg acgcgcccgt gttgcacgcg tcgccgcgcg tgctgcgttc gttcctcgac
    15241 atcgcggaga ccgtgctctg ccatcgcgat ccgcagcggc acgcgttgtt gtatcgcttg
    15301 ttgtggcgca tcgcccacgg cgagccgcat gtgctggcaa cgccgacgga tgccgacacc
    15361 cggcgggtga tgcaactggc gcaggaggtc cgccgcgacg cgcacaagat gaaggccttc
    15421 gtgcgcttcc gcgaagtgcc gggggagcgc gacaacttca tcgcctggtt cgagccatcg
    15481 catcacatcc tgcaccgggt cgcgccgttc ttcgcacgcc ggttcgccgg catgcgctgg
    15541 gcgatcctca ccccggaggg cagcgcgctg tgggatggcg aagccctgag cttcggtccg
    15601 ggcggtgcgc ccgacgatgc gcccgcgttc gatgccggcg agaacctgtg gcgcacctac
    15661 tacgcgcata tcttcaatcc ggcgcgcttg aacccgcgga tgatgcggca ggagatgccg
    15721 cagcgttact ggaagcatct gccggaagcg cacctgctgc cgtcgctgat ccagggcgcc
    15781 gcggtgcgcg tgcgcgagat ggcggagcgt gaacatgccg caccgaagcg ccgtattccg
    15841 gcgccaaccg tcgtgccgcc gaccgacgac acacgtcgcg acgagtcgct cgcgtcgctg
    15901 cgccaagcgg cacaggcatg ccgggcctgt cccctgtggc aaccggccac gcagaccgtg
    15961 ttcggcgaag gcccggagga cgcacgcgtc ctggtgctcg gcgaacaacc cggcgaccgg
    16021 gaagacctgt ccggccggcc gttcgtgggg ccggcgggca agctgctgga tcgcgcgctg
    16081 gccgaactcg gtatcgatcg ttcgcagttg tacctgacca atgcggtgaa gcatttccgc
    16141 ttcgagcggc gcggcaagca gcggctgcat cgcaaccccg aggcctcgca tgtgcaggcc
    16201 tgccgcagct ggctggccgg cgagatcgaa cgtgtccgcc cgcagatgat cgtctgcctg
    16261 ggtggcaccg ccgcgctggc ggtcttcggg gccgggtttc ggctgacggc gcaacgcggc
    16321 acctggcaga ccctgcacga cggcacccgc gccttcgcca ccgtgcatcc ggcgtggatc
    16381 ctgcgacagc ccgccgaccg gcaagcgggt gcctacgaag cgtggctgga cgacttgcgc
    16441 ctgctgctgg ccaccgccgg ctgatcgccg cgctctcgat ccgcgacgcg gatcacaaaa
    16501 gcaagtggca tgccaccggt ggttttggcg ttgaacgggt gaccgccatc cacagggaat
    16561 gcaatggaat tccgttcgat cctgctgctt gcactggcgc tgtgcgccgc gatcccggcc
    16621 gaggcgcaag tcgcgaagcg acagcccatc atcgacatgc acctgcatgt cctggcgccg
    16681 gatgcgcagg ggcctccacc gctgggcatg tgcacgccga tcgcggagat gccggcctgg
    16741 gatccgggcg cggggcccta cgtcgacacg atcatggcga tcttcaagaa gccgtcctgc
    16801 aaggacccgg tgtggtcgcc ggacacgcgc gaggagatgc gcgcgcgcac gctggccgag
    16861 ctggagaagc acgacgtcat cggcgtgttg agcggcgaca gcgacaagga catcgcggat
    16921 tggcgcaagg cgtcgccgga gcggatcatc ccggcgctgg gcgacagcat ccgcatgccc
    16981 gtggtcgatg ccgccacggt ggcgcgggtc aaggcgatga aggccagcgg cgacctggtc
    17041 gtgctcagcg agatcggccc gcagtacatg ggcatggcac ccgacgaccc gcgcctggag
    17101 ccgttctggg cgctggccga ggaactcgac ctgccggtcg gcatccacgt cggtaccgga
    17161 ccgcccggta ccccgtacct cgcgccgccg ctcaaggact atcgcgcgcg cctgcacagc
    17221 gcgctggtga tcgaagaggt gctggccagg cacccgaagc tgcgggtgta cctgatgcat
    17281 gccggctacc cgatgatcga cgacctgctg gcggtgctct acacgcaccc gcaggtctac
    17341 gtggacgtgg gcgtgatcgt ctacacgcag ccgcgaccgg cgttctatcg atatctgcag
    17401 gcgatcgtgg aggccggttt cgcgaagcgg gtgatgttcg gatcggacaa catggtctgg
    17461 ccggagacca tcggccgttc gatcgaggtg atccagcagg cgccgttcct cagcgaagcg
    17521 cagaagcgcg acatcttcta caacaacgcc gcgcgcttcc tgcgcctgga cgcggcgacc
    17581 atcgcgcggc accacgcact ctgatcgctc aaacgacaac gccgccttcc ggcggcgttg
    17641 tcgcatcgcg agtggcgtcg cttacttcgc cgtgaggcca cgcttctcca gcagcggctc
    17701 gatcttcgct tcgtagccgg agaagttcgg gaacagcttg ccggcttcga tctcgccgcc
    17761 aggcgccagc acgaacttgc gcaggcggtc gccattctgc aatgtcaggc cgccgttgtt
    17821 gcggatccac tgctcggtgt tcgcgcccag cacttccgac cagatgtagg cgtagtagcc
    17881 cgccgcgtag ccgcccatga tgtggctgaa gtagggcgtc ctgtaacgcg gcggcaccgg
    17941 cgcgtaatcg aagccgtcgg ccttgagtgc ctgcgcctcg aatgccatca cgccggacgc
    18001 ggccgggatc tgcgcggccg gcaactggtg ccagcgctgg tcgagcatcg cggcttccag
    18061 gtactcggtg gtggcgaagc cctggttgaa cttggccgcg gcgatcacct tgtccagcag
    18121 cgccttcggc atcggctcac cggtctggta gtgcttggcg tagttgccga gcacgctcgg
    18181 ccagtcggcc cacatctcgt tgacctgcga cgggtactcg acgaagtcgc gcggcacgtt
    18241 catcgagtaa tacggatagc gcacgtcctg gaagaagcca tgcagcgcgt ggccgaactc
    18301 gtggaacgcg gtggtgactt catcccaggt catcagcgtc ggcttgccgg cggacggctt
    18361 ggggatgttg aggtggttgg ccacgacggg cttgtcgccg gtcaggtcgg actgcgccac
    18421 gtaggtgttc atccaggcac cgccgcgctt ggaggaacgc gcatacgggt cccagatgaa
    18481 gatcgacagc aggctgccgt ccttgtcgta gacgtcgtag atccaggtgt ccgggtggta
    18541 cttcggcagg tcggtacgtt ccttgaaggt gatgccgtag agcttggtgg cggcgaagaa
    18601 cacgccatcc tccagcacgc gcttcatctc gaagtagggc ttgagctggg attcgtcgaa
    18661 gctgtacttc tgcgcgcgca ccttctcgct gtagtacgac cagtcccaag gtgccagctg
    18721 gaacgtcggc tggcgcttgg cctcctgctc ggcatcgatc atcgcctgca ggtcggcgcc
    18781 ctcgcggcgc gcgttgttca ccgcggccgg cgccagctgg cgcagcaggc gattcgcgtt
    18841 gtcctggttc gccgcggtct cgtcggccag cacgtagttg gcgtaggtgt cgtagcccag
    18901 cagggtggcc cgctcggcgc gcagcttcag cacctgcgag acgatcccgg tgttgtccca
    18961 ctggttgccg cggctgccgc gcgccaccga ggccttgtgc aggcgttcgc gcagcgcgcg
    19021 gttctcgagt tgcgcctccg ccggctggcc ggtggtgttg agcagggcga tcacgtactt
    19081 gccctccagt ccgcgggcct tggcggcctc cgcagcggcg gcgatctgct cttcgcccat
    19141 gcccttcagc tcgtcgcggc tgtcgaccac gatggcggaa tcgttcactt cggccagcac
    19201 gttctggttg aactgggtgc ccagcttgga caggtcggtg ttgatctcgc ggatgcgcgc
    19261 cttctgcgcg tcgtccagcg cagcgccgct gcgcacgaag tcggtgtagc gcttttccag
    19321 caggcgcagg tcgatggcat ccaggcccag cgtggcgcgc gcgtcatgca gcgtcttgat
    19381 gcgggcgaac agcttcggat ccagcgcgat ggcgtcgcgg tgggcggaga acttcggcgc
    19441 gtagtcggac tccagcttct cgcgggcgtc gttcttgtcg gtgccgatca ggttgaagaa
    19501 cacgttggtg gcgcgatcca gcacctgccc gctcttttcc atggcgacga tggtgttctg
    19561 gaagctcggc gcctccgggt tgttggcgat ggcctggatc tcgcgcagtt gctgcgccat
    19621 cccggcgtcg aaggcggggg cgaagtcgct gtccttgatc gcgtcgaatt gcgggtagtg
    19681 caggtcgagc gtgctgggtg cggcgaacgg gccgctgtag gttgccggca tcgtggcctc
    19741 cgaggtgttg gtcgcaggcg cggtggtggc gcagccggcc agtgcggtgg cgatcgcgag
    19801 cgcaagcgcg cagctgatgg ggtgcttcat tcgggcctcg tcaggcaggt tggcgaccgc
    19861 aaagcgtagc gcaggcgcat gggcagggcg cgtgacgaat ggcaggggca ttcacgccgc
    19921 ggcgaccgcg aggttccgat agtcgcgact tcccccgaat ggagatccgc catggcccag
    19981 gtcaccagcg tcatgaccgc cagcccttcc tgctgccgcg aggacacgcc actgcgagac
    20041 gtggcgcaac tgatgatcga ccatgactgc gggatgatcc cggtggtgga cgcagatggc
    20101 aggccggtgg gcgccgtcac cgaccgcgac atcgccgtcc gcatcgtcgc caggggcaag
    20161 gatgcgtcca gcagccgcgc cggcgatgcg atgaccagcc ccgtcaccac catcgacagc
    20221 gccaccagcc tgcacgatgc cacctgcgtg atggaggccg agaagatccg ccgggtcatc
    20281 gtggtcgatg ccgatggcaa gctggccgga gtggccgcca tcgcggacct ggccctggcc
    20341 ggcaagaacg aggcgacggc tgccgtcgtc agggaaattt cggagcccgg gagagcctga
    20401 gcgacgatcc atccatggga taggcgacga ccgcgggagc gcgcacaatc tgcgctcccc
    20461 cgtcgctgca ggttgccatg gccaccaccg cctacgattt cgacgccacc gccctggatg
    20521 gcacgccgca accgctttcc gactggcgcg gcaaggtcct gctgatcgtc aacgtggcca
    20581 gcaagtgcgg cttcaccccg caatacgccg ggctggagaa gctctggcgc gactatggcg
    20641 acaaggggct ggtggtgctg ggcttccctt gcgaccagtt cggtcaccag gagccgggca
    20701 acgccgagga aatccgcaat ttctgctcgt tgacctacga cgtcagcttc ccgatgttcg
    20761 ccaagatcga cgtcaacggg gcccacgcgc acccgctgtg gacctggctg aaggacgaga
    20821 agggcggcct gcttggtttc gatggcatca agtggaactt caccaagttc ctggtcggcc
    20881 gcgacggcaa ggtgatcaag cgctacgcgc cgaccgacaa gccggaatcg ctggcccgcg
    20941 acatcgagaa agcgttggga tgaccacgtc gccgccaggc gatgcctacc ggctgacctt
    21001 caagcaccag ccgccgttgc tgatagcgtg
//
LOCUS       DAOOCX010000036        20832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2224_length_20832_cov_8.406074, whole genome shotgun sequence.
ACCESSION   DAOOCX010000036 DAOOCX010000000
VERSION     DAOOCX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 20832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20832
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2224_length_20832_cov_8.406074"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..2656
                     /gene="alaS"
                     /locus_tag="PK399_05215"
     CDS             17..2656
                     /gene="alaS"
                     /locus_tag="PK399_05215"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPW12553.1"
                     /translation="MKTTSDIRRDFLEFFASKGHTIVPSAPLVPGNDPTLMFTNSGMV
                     QFKDVFLGAEKRSYVRAADVQRCLRAGGKHNDLDQVGYTARHHTFFEMLGNWSFGDYF
                     KKEAIAWSWELLTQVWGLPGERLLVTVYHNDDESYDIWNKDIGLPPERIVRIGDNKGA
                     PFASDNFWQMADTGPCGPCTEIFYDHGEHIAGGPPGSPDEDGDRYIEIWNNVFMQFDR
                     QPDGTLVPLPAPCVDTGMGLERLAAILQHVHTNYEIDLFQALIRHAAELTGTADLENK
                     SLRVIADHIRACAFLIVDGVLPSNEGRGYVLRRIIRRALRHGWMLGVREPFFHKLVAT
                     LDALMGDAYPVLREGRATAERALKAEEERFAETLDSGMRIFNDVAARSDGTIPGIDAF
                     RLYDTYGFPLDLTQDMARERGMSVDVAGFDAAMTQQKETARAAGKFGGGTQLPADLVA
                     QLTPTKFLGYDALTAGGLEVLALLKDGRPVESIAAGDDAIVILDASPFYAESGGQVGD
                     TGELDEQGVRFVVSDTLKLAGQFHGHVGKLSAGALKRGDRVSGTVDGGRRGATILNHS
                     ATHLLHAALRDVLGTHVQQKGSLVAPDRLRFDFSHFQPVTAAELAEIERRVNAEVRAN
                     HEGEVRHMAMQEALDFGAMALFGEKYGEHVRVLRFGPTSTELCGGTHVGRTGDIGLFK
                     IVSEGGVSAGVRRIEAVTGQGALDHVAEEERRLDEAAALLGGTSGDVADKLRALLDRQ
                     KKLERELEAIKAKAASGATSDLGSQARDVDGIKVLAARLEGFDAKALRDAMDRLKQQL
                     GDAVIVLAGTDGGKAALVAGVSGAATGKVKAGDLLGDVARQVNGKGGGRPDMAQGGGD
                     DGPALVAALDRVPAWVGERLAAH"
     gene            2772..2966
                     /gene="csrA"
                     /locus_tag="PK399_05220"
     CDS             2772..2966
                     /gene="csrA"
                     /locus_tag="PK399_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HPW12554.1"
                     /translation="MLILTRRVGETLMIGDAITVTVLGVKGNQVRIGITAPKDVAVHR
                     EEIYQRIQGDDGNKDGEAKG"
     gene            3024..3116
                     /locus_tag="PK399_05225"
     tRNA            3024..3116
                     /locus_tag="PK399_05225"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3058..3060,aa:Ser,seq:gct)
     gene            3204..3280
                     /locus_tag="PK399_05230"
     tRNA            3204..3280
                     /locus_tag="PK399_05230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3238..3240,aa:Arg,seq:acg)
     gene            3410..4459
                     /locus_tag="PK399_05235"
     CDS             3410..4459
                     /locus_tag="PK399_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW12555.1"
                     /translation="MQETPRRIKKKTRATSMDIAHRAGVSQATVSRVLRGSPLVNAET
                     RKRVEEAVRELNYKVDRHASSLRTQRAGTLALLLFEDPTPDDSHINPFFLSMLGSITR
                     ACAKHGHDLLVSFQQLSDDWHADYEDSMKADGLILLGYGDYIAYESKLAHLVAQGTHF
                     VRWGAVLQGQPGISIGCDNAAGGALVGRHLIERGRRKVAFLGDASTHFPEFFARYCGC
                     DGALREAGLRMNPALQVDADSSEQSGYDAARTLIGRGHAFDAIFAASDLIAIGAIRAL
                     SEHGLRVPEDVAIVGFDGTPMARFSNPPLTTVVQDTSGAGELLVASLLRLVRDEPAES
                     VTLMPTLEVRASSGG"
     gene            complement(4463..6211)
                     /locus_tag="PK399_05240"
     CDS             complement(4463..6211)
                     /locus_tag="PK399_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPW12556.1"
                     /translation="MTRSLLAAAIALLVGGCASTSPQDAASNRAPVKDYYGTLEPFAA
                     NAVYFVVTDRFVNGDTSNDHRDQGGGRANPKTRTFDIPLPPCNGVAGNIGYLGGDFKG
                     IADNLDYIRGMGFTSVWITPVVDNPDEAFTGGSPPGCGSILTDQGKSGYHGYWGTNFF
                     KLDEHLPSAHLDFAGLASAVHAKDMTLVLDIVGNHGSPGWTMPKPQPKFGQVFDQAGR
                     LVADHQNLPPQALDPAHNPLHRFYNTTGPVDAAKGSIFDGNLAQLADFDAGNPAVLDY
                     MVDAYGHWIDQGADAFRIDTISWMPPSFWDAFTRRIRAKHPGFFMFGEAFDYDAAKIA
                     THTLPGNGEVSVLDFPMKQAMDEVFGRKGAGYERLAPSLFLTDGPYANPYELATFYDN
                     HDMPRMDASDEGFIDAHNWLFTARGIPVVYYGSEMGFMRGRAEHAGNRNYFGNEGIAA
                     ARRSPIHAALARIANVRAATPALQRGVQVDIELQGNRAAFYRVLQHGDVRQVALVLLN
                     KGDAAQRFDISSLLQPGQWRSALDGGTLDVRAGGTLSTDVAAHGVQVFVLDAAVTMPA
                     LQAALDRAMTGARRPR"
     gene            complement(6232..7725)
                     /locus_tag="PK399_05245"
     CDS             complement(6232..7725)
                     /locus_tag="PK399_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW12557.1"
                     /translation="MHATGIANKPPLSFWQIWNMCFGFLGIQFGFALQNANVSRIFQT
                     LGASMDDIPILWIAAPLTGLLVQPIIGYMSDSTWTPLGRRRPYFLVGAVLASLALFAF
                     PNVPALWIAAGMLWVLDASINVSMEPFRAFVGDQLPAAQRPSGYAMQSFFIGVGSVVA
                     SMLPFLLEKVGVANTAAEGQVPDTVRYAFYAGGVVLLGAIAWTVLRTREYPPEELAAW
                     DEAPALERRLGSPGTVRAVAIAAIVVGAVIATVVQSRGLDKALYILAGLFAGFGIAFL
                     LRTLLSSTDNAFTRIIDDMRDMPSTMARLAVVQFFSWFALFAMWIYTTAAVTEVHFGS
                     TDASSAAYNEGANWVGVLFAAYNGFAALAAIAIPWMVRALGLRMSHLVNSVLGGLGLL
                     SMLWIRDPQWLLLSMVGVGFAWASILSLPYALLSDSVPAAKMGIYMGIFNFFIVIPQL
                     VAASLLGFLLKTWLGGHPINALAIGGTSMLIAGLCVLLVRAPATRTA"
     gene            7924..9537
                     /locus_tag="PK399_05250"
     CDS             7924..9537
                     /locus_tag="PK399_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase family protein"
                     /protein_id="HPW12558.1"
                     /translation="MTDQQWWRGAVIYQIYPRSFLDTDGDGVGDLPGIVARLDHVASL
                     GVDAIWISPFFKSPMQDFGYDISDYCDVDPLFGTLDDFDRLLREAHARGLKVMIDQVP
                     SHCSIEHPWFVESRQDRTNPKADWFVWADAKPDGSPPNNWLSIFGGVAWTWEPRRRQY
                     YLHNFLSSQPQLNFHNPQAYAAQLDTLRFWLDRGVDGFRLDSINFPFHDAQLRDNPAK
                     PLELRQGRGFSPDNPYAFQYHTYNNTRPETLGFLEDVRRLMDGYADVAALGEISSDDS
                     LATMTDYVQPHRLHMGYSFELLTDDGSPAHIRATVEALESQMQGGWPCWAISNHDVQR
                     AVTRWGTGQDDDAHARQLLALVCALRGSVCLYQGEELGLPEAEVPYEALRDPYGKAFW
                     PSFKGRDGCRTPMPWAAHAHAGFSDATPWLPVPAAHIARNVEAQAADAGSVLNAARAF
                     LGWRKSQPALVEGTIRFLDAPEQLLAFVREHGDQRLLVAFNLSRQAIAWTPPAASLVL
                     LPAPGVDAAGLREGVLHFPPRGAAFASLA"
     gene            9555..12281
                     /locus_tag="PK399_05255"
     CDS             9555..12281
                     /locus_tag="PK399_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3372 domain-containing protein"
                     /protein_id="HPW12559.1"
                     /translation="MRTTFALLLAVAGNAQAGTADCDGDAARVLHAAAHAATDARAYW
                     LDGTRIRWPAQPADARYRLVASSSAGLRVERGQAVAGADRSYLLQASHDLPDAVAARF
                     RFVGEGATLRLPDEAKAGLRDLLRGQAMLVREDAQGTVVDATYLQLPGVLDALYAEAA
                     ADRAPLGATPGDGATWFRLWAPTARDVSLCLYRDGASAAIELVALARDDRTGQWLDGI
                     PHDLRGGYYTYLVDVFVPGIGIVRNRVTDPYSASLTTDSRRSYIVDLDDPALQPAGWE
                     DAPRPPALASNTDMAIYELHVRDFSIGDASVPTSHRGKYLAFTDAGSNGMRHLRALGD
                     AGITDIHLLPVFDIATIPEAGCATPTIPKAAADSDAQQAAVMAVASKDCFNWGYDPFH
                     FNAPEGSYASDAADGAVRIREFRAMVQALHAAGLRVGMDVVYNHTTASGQSPRSVLDR
                     IVPGYYQRLDANGKVETSTCCDNTATEHAMMARLMRDSVASWARDYRIDSFRFDLMGH
                     QSRDAMVEVQRAADAAAGRHIPLVGEGWNFGEVANGARFPQAAQGMLNGSGIASFSDR
                     ARDALRGGGCCDGGIDLFAQQGLLNGLAYAPNASARGRATRRDLLHAADLARAGLAGT
                     LRDYRTTFADGRRASLSALDYAGMEAGYASQPGEVVNYVENHDNPTLWDIHALKLPQA
                     TSASERARVQLLGAAFVAFSQGIAYFHAGMDVLRSKSLDRNSFDSGDWFNRLDWSYTN
                     NGFGAGLPPEQDNGKDWALLRPVLRNPNAKPSPVDIAWMRDAFRDLLRIRASTPLLRL
                     GSADDVQRRLAFRNVGPKQDPLVVVGHLDGDGMPGAYREVLYLLNVSPETRTLALPEE
                     VGKAYALHPVQAADGAADARAKQARYAAKDGTFTVPGRTAVVFVVETKKDRQ"
     gene            12278..14314
                     /locus_tag="PK399_05260"
     CDS             12278..14314
                     /locus_tag="PK399_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HPW12560.1"
                     /translation="MKALKFGILLALALPGFVHAEQVAQATSPDGRIVVQLDLNSEGR
                     LAYRVLRDGKPVIADSRLGFILRNDRQLLRNLQLDTQSSSSFDETWEQPWGERRFVRN
                     HYNELRADFAEKDRDHRRFSMVFRVFDDGVGFRYDVPKQPGLAEAKIVQELTEFAVAR
                     PSTAWWIPAYQWNREEYLYNRTPLSQVGVAQTPITIRADDGLHVTLHEAALVDYAGMN
                     LAKGGGQVLHASLTPGSGPAPVVRATPFQTPWRTIQIGERAADLVESSLVLNLNEPNR
                     LGDVGWFKPHKYVGVWWSLHLDKETWATGPKHGATTENTKRYIDFAAANGFRGVLVEG
                     WNVGWDGDWFANGWDFDFRKPTPDYDLRGLAAYAKAKGVHLIGHHETGCAVSHYERQM
                     DEAFALFDTIGIDAVKTGYVCDAGQIERQDVAKGPVLREWHEGQWMSNHHLRVVQDAA
                     RHHVAINAHEPIKDTGLRRTYPNWISREGARGQEFNAWGDPPNTPEHEVTLVYTRMLA
                     GPMDYTPGVVSLTGKNGQEIGSTLARQLALYVALYSPIQMAADLPEHYAQHPDAFRFI
                     KDVAVDWDESHLIAGEVGEYAAIARKQRGDAAWFIGAINDRHARTVPLRLDFLDAGKR
                     YRAEIYRDGEGADWQGDARFRFVREERTVRRGDVLDAWLAGGGGIAVRLVPLAR"
     gene            14621..17392
                     /locus_tag="PK399_05265"
     CDS             14621..17392
                     /locus_tag="PK399_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015463361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW12561.1"
                     /translation="MVQLKRNLLSVALATATMMLAAQAHAQSAPAADQAGQEAAAKKK
                     DDAKNLDTVVVTGIRGGIERAIDIKKESTSIVEAISAEDIGKLPDSSIAESISRLPGI
                     AAQRVAGRAQVISVRGLSPDFATTLLNGREMVSTGDNRSVEFDQYPSELMAGVTLYKT
                     PDAALVGQGLSGTIDMRTVRPLDYDNAVFAVSGRLTRSSLGSAANADANGNRFNVSYI
                     GQTADRKVGFSIGYSHSDTPIQENQVGLYEPWQAIGDNWRPGVPAGTYYSDGIKALRR
                     TGYTKRDAVMGTLEFRPSDAWISTLDVFHSEAKQEDTANQFEVHIGDYNGGYGRLNVL
                     NPRIVNGTFVGGTAQNVYPLVRGMYNNRKDEINAFGWRNEFFVGRAKLVADVSWSKSD
                     RDELNLENNLQLAPAPQLDSVVLDFGGGGFPQLDPGRDYSNPATLFLRNTIYGSGYGK
                     TPKVADELKGFKVYASLPAPEAAANFFSDIDIGLNYADREKQKWQPEGNINVGAQGDT
                     TIGSEFQYGLVDLGFAGVGLIPSWDVPGVVAKYMTFNPTDDTAAYLIPKAWSVSETIT
                     TGYIKGNIDTTWGSVGVRGNIGVQVQHTDQSSASRVWDSSRPAGSEIRPYELGDKYTD
                     VLPSMNLAFSFENDQTLRVALARQVARARIDQMRASLEFGVNTATGEPGAGGGNPLLQ
                     PWRANAFDISYEKYFGTKAYVAAAYFYKDLRSYIFTQGRDGYDFTDLLVGYVPPPGSA
                     PVKNIGRMTAPFNGKGGMLQGLELSASLPLDMLWEPMRGFGIVASASFTDSSIEILAP
                     ENASSVGNGPIALPGLSKRVYNLTAYYERNGFEFRVNNRRRSDFIGEIGNFAGERSLR
                     YVVGENITDAQVSYSFGEGSSLKGLSLLLQASNLGNETYQTYAGSKDRPLENIEWGRT
                     LLLGASYKF"
     assembly_gap    17431..17530
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17531..18132)
                     /locus_tag="PK399_05270"
     CDS             complement(<17531..18132)
                     /locus_tag="PK399_05270"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPW12562.1"
                     /translation="MFDTARHSELVDAVFGLAPVIPVLAIDRIEDALPLCRALADNGL
                     PVLEITLRTACALDAIALVARELPHACVGAGTVLSATDLANVADAGARFAISPGATDT
                     LFDAAKASRTPLIPGIATASELMRGMEHGWRRFKFFPAESSGGIGALKGFGGPLPMAR
                     FCPTGGIDAAKAPAYLALANVACVGGSWMVPADAVKAGDWA"
     gene            complement(18138..19151)
                     /gene="glk"
                     /locus_tag="PK399_05275"
     CDS             complement(18138..19151)
                     /gene="glk"
                     /locus_tag="PK399_05275"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HPW12563.1"
                     /translation="MTANTLALVGDIGGTNARFALTDLSSPTIELLESRALPNADFAS
                     MQHAIEHYLSGASAKPARAALAVACPVGNDEIRLTNRAWSFSRSELQRTLGLHELRML
                     NDFGAVAWAIPALQREHLVTLHGDPAAPLRGPISVLGPGTGLGVALIVGSREHGWHAV
                     ETEGGHVGFSPVGDEERAISAWLTAQHGRTSTERLLCGKGLSEIDVVLRGASAVLPGP
                     SLLPGETSLLKPALREPAEIVAAALEGHDQAARRTLARFCSVLGSVAGDCALIHGART
                     VVIAGGIVPRFIPFLRASAFRERFLAKGRMAALLESVPIHVITHPHPGLLGAATALRA
                     KEF"
     assembly_gap    19183..19282
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19283..>20832)
                     /gene="edd"
                     /locus_tag="PK399_05280"
     CDS             complement(<19283..>20832)
                     /gene="edd"
                     /locus_tag="PK399_05280"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HPW12564.1"
                     /translation="RNAGGSAQVAGGVPAMCDGVTQGRTGMELSLFSRDAIAMATAIS
                     LSHDMFDAVLCLGVCDKIVPGLLIGALSYGHLPVIFVPAGPMPSGIPNKQKAEVRQRY
                     ALGQATRDELLEAEAASYHSPGTCTFYGTANSNQMLMEVMGLHMPGSAFVPPNTPLRD
                     ALTVAATEQALRITALGNDYRPIGRTIDEKAIVNAMVGLAATGGSTNHALHLVAMARA
                     AGLLIDWTDLDELSRATPLLARVYPNGSADVNHFEAAGGLGLVINELLDAGLLHGDIA
                     CVHGGDLRQQATQPWLDDLSLRWRTAPATSLDENVVRGVAAPFDSEGGLRCLQGNLGR
                     AVVKISAVAPELRRIEAPARIFESQDALLAAFKVGALDRNEGFAGDFVAVVRGQGPRA
                     NGMPELHKLTPTLAALQDRGQRVALLTDGRMSGASGKVLAAIHVTPEAADAGPLAKLR
                     DGDLVRIDGDAGTMEALVDAGAWAAREAVRPALAGNHVGHGRELFAMMRKQVGSAEQG
                     ACALFLDE"
BASE COUNT         3475 a         6841 c         7054 g         3262 t
ORIGIN      
        1 cctgtaaaat ggccggatga aaacgacttc cgatatccgc cgggacttcc tcgagttctt
       61 cgcctccaag ggccacacca tcgtgccgtc cgcgccgctg gtgccgggca acgacccgac
      121 cctgatgttc accaactcgg gcatggtcca gttcaaggac gtgttcctcg gtgccgagaa
      181 gcgcagctac gtacgcgcgg ccgacgtgca gcgctgcctg cgcgcgggcg gcaagcacaa
      241 cgacctcgac caggtgggct acaccgcgcg ccatcacacc ttcttcgaga tgctcgggaa
      301 ctggtcgttc ggcgattact tcaagaagga ggccatcgcc tggtcctggg agctgctgac
      361 ccaggtctgg gggctgcctg gcgagcgcct gctggtcacg gtgtaccaca acgacgacga
      421 gtcctacgac atctggaaca aggacatcgg actgccgcct gagcgcatcg tgcgcatcgg
      481 cgacaacaag ggtgcgccgt tcgcctccga caatttctgg cagatggccg acaccggtcc
      541 ctgcggtccg tgcaccgaga tcttctacga ccatggcgag cacatcgccg gtggcccgcc
      601 cggttcgccg gacgaggatg gggatcgcta catcgagatc tggaacaacg tgttcatgca
      661 gttcgaccgc cagccggacg gcaccctggt gccgctgccg gcgccttgcg tcgacaccgg
      721 catgggcctg gagcgcctgg ccgcgatcct gcagcacgtg cacaccaact acgagatcga
      781 cctgttccag gcgctgatcc ggcatgccgc cgaactgacc ggcaccgccg acctcgaaaa
      841 caagtcgttg cgggtgatcg ccgaccacat ccgtgcctgc gccttcctga tcgtggatgg
      901 cgtgctgcca tcgaatgaag gccgcggcta cgtgttgcgc cggatcatcc gccgcgcgtt
      961 gcgccatggc tggatgctgg gcgtgcgcga gccgttcttc cacaagttgg tggccacgct
     1021 ggatgcgctg atgggcgatg cctacccggt gctgcgcgag ggccgcgcca ccgccgagcg
     1081 tgcactcaag gccgaggaag aacgcttcgc ggaaacgctc gattcgggca tgcgcatctt
     1141 caatgacgtg gcggcgcgaa gcgacggcac catcccgggc atcgatgcct tccgcctgta
     1201 cgacacctac ggcttcccgc tcgacctgac ccaggacatg gcccgcgaac gtgggatgtc
     1261 ggtggacgtc gccggcttcg atgccgcgat gacccagcag aaggaaaccg cgcgtgccgc
     1321 cggcaagttc ggtggcggca cccagttgcc ggcggatctg gtcgcgcagc tgacaccgac
     1381 gaagttcctc ggctacgacg cgttgaccgc gggcgggctg gaagtgctgg cgctgttgaa
     1441 ggacggtcgt cccgtcgagt ccatcgccgc cggcgacgat gccatcgtca tcctcgacgc
     1501 aagcccgttc tatgccgaat cgggtggcca ggtcggcgat accggcgaac tcgatgagca
     1561 gggcgtgcga ttcgtcgtca gcgacaccct gaagctcgcc ggccagttcc atggccatgt
     1621 cggcaagctg tccgccggcg cactcaagcg cggcgaccgg gtgtcgggta cggtcgatgg
     1681 tgggcgccgc ggcgcgacga tcctcaacca cagcgccacc catctcctgc atgccgcact
     1741 gcgcgacgtg ctgggcaccc acgtgcagca gaagggctcg ctggtcgcac cggatcgcct
     1801 gcgcttcgac ttctcgcact tccagccggt gactgcggcg gagcttgccg agatcgagcg
     1861 ccgggtgaac gccgaagtgc gcgcaaacca cgagggcgaa gtgcgccaca tggcgatgca
     1921 ggaagcgctg gacttcggcg cgatggcgct gttcggcgag aagtacggcg agcacgtgcg
     1981 cgtgttgcgc ttcggcccga cctcgaccga actgtgcggt ggcacccatg ttggccgcac
     2041 cggcgacatc ggcctgttca agatcgtgtc cgaaggtggc gtgtccgccg gcgtgcgccg
     2101 catcgaagcg gtgaccgggc agggcgcact ggatcatgtg gccgaggaag agcgtcgcct
     2161 cgacgaggcc gccgccttgc tgggtggcac cagcggcgac gtggccgaca agctgcgcgc
     2221 attgcttgac cgccagaaga agctggagcg cgaactggag gcgatcaagg ccaaggcggc
     2281 ctcgggcgcg acgagcgatc tcggcagcca ggctcgcgac gtggacggga tcaaggtgct
     2341 cgcggcgcgc ctggaaggct tcgatgccaa ggcattgcgc gatgcaatgg atcggctcaa
     2401 gcagcaactg ggcgatgcgg tcatcgtgct ggccggcacc gacggtggca aggcggcgct
     2461 ggtggcgggc gtgagcggag ccgccaccgg caaggtcaag gccggcgacc tgctgggcga
     2521 cgtggcccgc caggtcaacg gaaaaggggg tggccgcccc gacatggccc agggcggtgg
     2581 cgacgacggc ccggcgctgg tggccgcgct cgatcgcgtg ccggcgtggg ttggcgagcg
     2641 cctcgcggcg cattgaatgc agcgaccgtg atggcggtcg cttgtgcaag ctgaacgatg
     2701 ccccgtatga tcgggcggat tggatgcatc tggccgcacg tttccgtggc ggctgcgaca
     2761 ggggagttgc gatgctgatc ttgaccaggc gggtaggcga aaccttgatg atcggcgatg
     2821 ccatcaccgt cacggtgctt ggcgtcaagg gcaaccaggt tcgcatcggc atcaccgcgc
     2881 cgaaggatgt cgccgtgcat cgcgaggaga tctaccagcg catccagggc gatgacggca
     2941 acaaggacgg cgaggcgaaa ggctgaggtt tgccggatcg cggtacaacc tgtattcttc
     3001 gccgctcgcg atttcgccgc accggagact tgcccgagtg gccgaagggg ctcccctgct
     3061 aagggagtat ggggtcaaaa gctccatcga gggttcgaat ccctcagtct ccgccagtcc
     3121 ggtgaaaccg cgaaacaagc gtcatcgaag tgatttttcg ttgacgtgga atcatgcagc
     3181 aacgcataat tccgcttctg catgcgcccg tagctcagtt ggatagagca ccaggctacg
     3241 aacttggggg tcgggagttc gaatctctcc gggcgcacca ccattgaaga aagccggcct
     3301 ttggccggct tttttttcgc ccgtcgcgtc gtcctgccgc gagtggagcg ggacgcgcgc
     3361 tgcgcccgcc gcggcggccg cgcgcgcggg tgattgctac actgcagcga tgcaggaaac
     3421 gccgcgacgc atcaagaaaa agacccgcgc cacttcgatg gacatcgccc atcgcgccgg
     3481 ggtgtcgcag gccaccgtct cgcgcgtgtt gcgcggcagc ccgctggtca acgccgagac
     3541 ccgcaagcgg gtcgaggaag cggtccgcga gctgaactac aaggtcgatc gccacgcatc
     3601 gagcctgcgc acccagcgcg caggcaccct ggccctgctg ctgttcgagg acccgacgcc
     3661 cgacgactcg cacatcaatc cgttcttcct gtcgatgctc ggctcgatca cccgcgcctg
     3721 cgccaagcac gggcatgacc tgctggtctc gttccagcag ctgtcggacg actggcatgc
     3781 cgactacgaa gacagcatga aggccgatgg cctgatcctg ctgggctatg gcgactacat
     3841 cgcctacgag agcaagctgg cgcacctggt cgcgcaaggc acgcatttcg tccgctgggg
     3901 cgcggtgctg caggggcagc cggggatctc gatcggctgc gacaatgccg ccggtggcgc
     3961 gctggtcggg cgccacctca tcgagcgggg acgtcgcaag gtcgcgttcc tcggcgacgc
     4021 ctcgacccat ttcccggagt tcttcgcccg ttactgcggc tgcgatggcg cgttgcgcga
     4081 ggccggcctg cgcatgaacc cggccttgca ggtcgatgcc gacagttccg agcaatccgg
     4141 ctacgacgca gcgcgtacgt tgatcggtcg cggccatgcc ttcgatgcga tcttcgccgc
     4201 cagcgacctg atcgcgatcg gcgcaatccg ggcgctcagc gaacacggcc tccgggtgcc
     4261 tgaagacgtg gccatcgtcg gcttcgacgg cacgccgatg gcgcgcttct ccaatccgcc
     4321 gttgaccacg gtggtgcagg acacctcggg ggccggcgag ttgctggtcg ccagcctgct
     4381 gagactggtg cgcgacgagc ccgcggagag cgtgaccctg atgccgacgc tggaagtccg
     4441 cgcctccagc ggcggctgac cctcagcgcg gtcggcgcgc gccggtcatc gcgcggtcca
     4501 gcgcggcctg cagcgcaggc atcgtgacgg ccgcatccag cacgaacacc tgcacgccat
     4561 gcgccgcgac gtcggtcgac aacgtgccgc ccgcacggac atcgagcgtg ccgccgtcga
     4621 gcgcggagcg ccattgtcct ggctgcagca gcgaagagat gtcgaagcgc tgcgcggcat
     4681 cgcccttgtt gagcagcacc agtgcgacct gccggacgtc gccatgctgc agcacgcgat
     4741 agaacgccgc gcgattgccc tgcagctcga tgtcgacctg cacgccgcgc tgcaatgcgg
     4801 gtgtggccgc acgtacgttg gcgatgcgcg caagcgccgc gtggatcggg ctgcgccgcg
     4861 ccgcggcgat gccttcgttg ccgaagtagt tgcgattgcc ggcatgctcg gcgcggccgc
     4921 gcatgaagcc catctcggag ccgtaataga ccaccgggat gccgcgtgcg gtgaacagcc
     4981 agttgtgcgc gtcgatgaag ccttcgtcgc tcgcatccat ccgcggcatg tcgtggttgt
     5041 cgtagaacgt cgccagctcg taggggttgg cgtacggccc atcggtgagg aacaacgatg
     5101 gcgcaagacg ctcgtaaccc gcgcccttgc gcccgaacac ctcgtccatc gcctgcttca
     5161 tcgggaagtc gagcacgctg acttcgccgt tgccgggcag ggtatgcgtc gcgatcttcg
     5221 ccgcgtcgta gtcgaaggct tcgccgaaca tgaagaagcc gggatgcttc gcgcggatgc
     5281 gtcgggtgaa tgcatcccag aacgacggcg gcatccagct gatggtgtcg atgcggaagg
     5341 cgtccgcccc ctggtcgatc cagtggccgt aggcatcgac catgtagtcg agcaccgcgg
     5401 ggttccccgc atcgaaatcc gcgagttgcg ccagattgcc gtcgaagatc gatcccttcg
     5461 cggcgtcgac cgggccggtg gtgttgtaga agcgatgcag cgggttgtgc gcgggatcca
     5521 gcgcttgcgg cggcaggttc tggtggtcgg cgacgagcct gccggcctgg tcgaacacct
     5581 ggccgaactt cggctgtggc ttcggcatcg tccagcccgg cgagccgtgg ttgccgacga
     5641 tgtccaggac caatgtcatg tccttggcat gcaccgcact ggcaagtccg gcgaagtcca
     5701 ggtgggcgct cggcaagtgt tcgtcgagct tgaagaaatt cgtgccccag tagccgtggt
     5761 agccgctctt gccctggtcg gtgaggatgc tgccgcaacc cggcggcgag ccaccggtga
     5821 aggcttcgtc cggattgtcg acgaccggcg tgatccacac cgaggtgaag cccatcccgc
     5881 gaatgtagtc caggttgtcg gcgatgccct tgaagtcgcc gcccaggtag ccgatgttgc
     5941 cggccacgcc attgcacggc ggcagcggga tgtcgaaggt gcgcgtcttg ggattggcgc
     6001 gtccgccgcc ctggtcccga tgatcgttcg acgtgtcgcc gttgacgaag cgatcggtga
     6061 ccacgaagta caccgcattc gcggcgaacg gttccagcgt gccgtagtaa tcctttacgg
     6121 gtgcgcggtt cgatgccgca tcttgtggcg acgtcgatgc gcagccgccg acgagcaggg
     6181 cgatggccgc ggcgagcagg gagcgtgtca ttggcatgtc ggtcggcctc ctcaggcggt
     6241 gcgggtggcg ggggcgcgca ccagcaacac gcacaggccg gcgatgagca tgctggtgcc
     6301 gccgatcgcc agcgcattga tcggatggcc gcccaaccag gtcttcagca ggaagccgag
     6361 caggctggcc gcgaccaact gcgggatcac gatgaagaaa ttgaagatgc ccatgtagat
     6421 gcccatcttc gcggccggca cgctgtcgga gagcaacgca tatggaagcg acaggatcga
     6481 cgcccacgcg aaacccacgc cgaccatcga cagcaacaac cactgcggat cgcggatcca
     6541 cagcatcgac aacaggccca gtccgccgag cacggagttg accaggtggc tcatgcgcag
     6601 gccgagcgcg cgcaccatcc acgggatcgc gatggccgcg agcgcggcga agccgttgta
     6661 ggcggcgaac agcacgccca cccagttcgc gccttcgttg tacgcggccg agctggcgtc
     6721 ggtgctgccg aaatggacct cggtcactgc cgcggtggtg tagatccaca tcgcgaacag
     6781 cgcgaaccag gagaagaact gcacgacggc gagccgtgcc atcgtcgaag gcatgtcgcg
     6841 catgtcgtcg atgatgcggg tgaacgcatt gtcggtggag gacagcagcg tgcgcagcag
     6901 gaaggcgatg ccgaagccgg cgaacaggcc ggccaggatg tacagggcct tgtcgagccc
     6961 gcgcgactgg accaccgtgg cgatgaccgc gccgaccacg atcgcggcga tggccaccgc
     7021 acgcaccgtg ccggggctgc cgaggcggcg ttcgagcgct ggcgcttcgt cccacgccgc
     7081 gagttcctcc ggcggatatt cgcgggtgcg cagcacggtc caggcgatcg cgcccagcag
     7141 caccacgccg ccggcgtaga acgcgtagcg cacggtgtcc ggcacctgtc cctcggccgc
     7201 ggtgttggca acgcccacct tctccagcag gaacggcagc atgctggcga ccaccgatcc
     7261 cacgccgatg aagaagctct gcatcgcgta gccggacggc cgttgcgcgg ccggcaactg
     7321 gtcgccgacg aaggcgcgga acggttccat cgaaacgttg atggaggcat ccaggaccca
     7381 cagcatgccc gcggcgatcc acagcgcagg cacgttgggg aaggcgaaca gcgccagcga
     7441 ggcaagcacc gcgccgacca ggaaatacgg gcgacgacgc ccgagcggcg tccatgtgct
     7501 atcggacatg tagccgatga tcggttgcac cagcaggccg gtgagcggcg ccgcgatcca
     7561 caggatgggg atgtcgtcca tgctcgcgcc gagcgtctgg aagatgcggc tcacattggc
     7621 gttctgcagc gcgaatccaa actggatccc caggaagccg aagcacatgt tccagatctg
     7681 ccagaacgac agtggcggct tgttggcgat gccggttgcg tgcatgcgtg tccccgtgtg
     7741 cgtgcggccg aaggtgcgcg gagtgtgctg cagcacatcg gtcgagtcgt gatgcagcgc
     7801 cgcatattcg gcatgtttca tggggtcatc gcgcatgtaa acgtattcaa gccatcgtcg
     7861 cgcgggcgac acgcgttgca cgcggcatga tccgcggcag tcgcaggcgg ggtggacgtg
     7921 gggatgacgg atcagcaatg gtggcgcggc gcggtgatct accagatcta cccgcgcagc
     7981 ttcctcgaca ccgatggcga cggcgtgggc gacctgccgg gcatcgtcgc gcgcctcgac
     8041 cacgtggcgt cgctgggcgt cgatgcgatc tggatctcgc cgttcttcaa gtcgccgatg
     8101 caggatttcg gctacgacat ctccgactac tgcgacgtcg atccgttgtt cggcaccctg
     8161 gacgacttcg accgcctgct gcgcgaagcc cacgcgcgcg gcctgaaggt gatgatcgac
     8221 caggtcccca gccattgctc catcgagcat ccttggttcg tcgaaagccg gcaggaccgc
     8281 acgaatccga aggccgactg gttcgtctgg gccgatgcca agccggatgg cagcccgccc
     8341 aacaactggc tgtcgatctt cggcggcgtg gcctggacct gggagccgcg ccggcgccag
     8401 tactacctgc acaatttcct gagctcgcag ccgcagctga atttccacaa cccgcaggcg
     8461 tacgcggcgc agctggacac cctgcgtttc tggctggatc gcggtgtcga cggcttccgc
     8521 ctggattcga tcaacttccc gttccacgac gcgcaactgc gcgacaaccc ggccaagccg
     8581 ctggaactgc ggcagggacg cggcttcagc cccgacaatc cgtatgcgtt ccagtaccac
     8641 acctacaaca acacgcgacc ggagaccctc gggttcctgg aagacgtacg ccgcctgatg
     8701 gacggctatg ccgatgtcgc cgcgctcggc gaaatctcct cggacgactc cctcgccacg
     8761 atgaccgact acgtgcagcc gcatcggttg cacatgggct acagcttcga gctgctgacc
     8821 gatgacggtt cgcccgcgca catccgcgcg acggtcgaag cgctcgagtc gcaaatgcag
     8881 ggcggctggc cgtgctgggc gatctccaac cacgatgtgc agcgcgcggt cacccgctgg
     8941 ggcacgggcc aggacgacga tgcgcatgcg cgccagttgc tggcgctggt ctgcgcgttg
     9001 cgtggatcgg tctgcctgta ccagggcgag gaactcggct tgccggaagc cgaggttccc
     9061 tacgaagcgt tgcgggatcc gtacggcaag gcgttctggc ccagcttcaa gggccgcgac
     9121 ggttgccgca cgccgatgcc gtgggccgcg cacgcacatg ccggcttcag tgatgccacg
     9181 ccgtggttgc cggtgccggc tgcgcacatc gcgcgcaacg tggaagcgca ggcggccgat
     9241 gccggctcgg tgttgaacgc cgcgcgtgcc ttcctgggct ggcgcaagtc gcaacccgca
     9301 ctggtcgagg gcacgatccg tttccttgat gcgccggagc aactgctcgc gttcgtgcgc
     9361 gagcatggcg accagcgcct gctggtggcc ttcaacctgt cgcggcaggc catcgcatgg
     9421 acaccgcccg ccgccagcct ggtcttgttg ccagcgcccg gcgtggatgc ggccggcctg
     9481 cgcgagggcg tgctccactt cccgccgcgc ggcgcggcgt tcgccagcct ggcgtgagca
     9541 aaccgatgcg cggcatgcga acgaccttcg cattgctgct tgccgtcgcc ggcaacgcgc
     9601 aggccggcac tgcggactgc gatggcgacg ccgcgcgcgt gctgcacgcg gcggcgcacg
     9661 cggccaccga cgcacgcgcg tactggctgg acggcacccg tatccgttgg ccagcccagc
     9721 cagccgacgc gcgttaccgg ctggtggcgt cgtcttcggc cggcctgcgg gtcgagcgcg
     9781 ggcaggcggt cgccggcgcg gatcgcagtt acctgttgca ggcctcgcac gacctgcccg
     9841 atgcggtcgc cgcgcgcttc cgcttcgtcg gcgagggcgc gaccttgcga ctgccggatg
     9901 aggcgaaggc ggggctgcgc gatctcctgc gcggccaggc gatgttggtg cgcgaggatg
     9961 cgcaaggcac ggtggtcgat gccacctacc tgcagctccc cggcgtgctg gacgcgttgt
    10021 acgccgaggc ggccgccgat cgcgcgccgc tgggcgccac ccccggcgac ggggcgacat
    10081 ggttccgcct gtgggcgccc accgcgcgcg acgtgtcgct gtgcctgtat cgcgatggcg
    10141 catcggccgc gatcgaactg gtggcgctgg cgcgcgacga ccgcaccggc caatggctcg
    10201 acggcatccc gcatgacctt cgtggcgggt attacaccta cctggtcgac gtgttcgtgc
    10261 ccggcatcgg catcgtgcgc aatcgcgtca ccgatccgta ttcggccagc ctcaccacgg
    10321 actccaggcg cagctatatc gtcgacctcg acgatcccgc gttgcagccc gcgggttggg
    10381 aagacgcgcc gcgcccgccg gcgctggcgt ccaacaccga catggcgatc tacgaactgc
    10441 acgtgcgcga tttctcgatc ggcgatgcga gcgtgccgac gtcgcatcgc gggaaatacc
    10501 tcgccttcac cgatgccggc agcaacggca tgcgccacct gcgcgcgctg ggcgacgccg
    10561 gcatcaccga catccacctg ttgccggtgt tcgacatcgc cacgatcccg gaagccggct
    10621 gcgccacgcc gacgatcccg aaagcggccg cggacagcga cgcgcaacag gccgcggtga
    10681 tggcggtggc atcgaaggac tgcttcaact ggggctacga cccgttccac ttcaacgcgc
    10741 cggaaggcag ttacgccagc gatgcggccg acggcgcggt gcgcatccgc gagtttcgcg
    10801 cgatggtgca ggccttgcac gcggccggcc tgcgcgtggg catggacgtg gtctacaacc
    10861 acaccaccgc atccggccag tcgccgcgct cggtgctgga ccggatcgtc cccgggtact
    10921 accagcggct ggatgccaac ggcaaggtcg agacctcgac ctgctgcgac aacaccgcca
    10981 ccgaacacgc gatgatggcg cggctgatgc gcgattcggt cgcgtcgtgg gcgcgtgact
    11041 accgcatcga ttcgttccgc ttcgacctga tgggccacca gtcccgtgac gccatggtcg
    11101 aggtgcagcg cgccgcggat gcggccgccg gtcgccacat cccgctggtg ggcgagggct
    11161 ggaacttcgg cgaggtcgcc aacggcgcgc gcttcccgca ggccgcacag ggcatgttga
    11221 acggcagcgg gatcgccagc ttcagcgatc gcgcccgtga tgccttgcgc ggtggcggct
    11281 gctgcgacgg cgggatcgac ctgttcgcgc aacagggtct gctcaacggt cttgcctacg
    11341 cgccgaatgc cagtgcacgc ggcagggcca cgcgccgcga cctgctgcat gccgcggatc
    11401 ttgcgcgcgc cggcctggcc ggcaccttgc gcgactaccg caccacgttc gcggatggcc
    11461 gccgcgcatc gctgtccgcg ctggactacg ccggcatgga agccggctat gccagccagc
    11521 ccggcgaggt ggtgaactac gtcgagaacc acgacaaccc cacgctgtgg gatatccatg
    11581 cattgaagct gccgcaggcg accagtgcat ccgaacgcgc gcgggtgcaa ctgctgggcg
    11641 cggccttcgt cgccttcagc cagggcatcg cgtatttcca cgccggcatg gacgtgttgc
    11701 gcagcaagtc gctggatcgc aacagcttcg attccggcga ctggttcaat cggctcgact
    11761 ggagttacac caacaacggt ttcggtgctg gattgccgcc agagcaggac aacggcaagg
    11821 actgggccct gcttcggccg gtgctgcgca acccgaatgc gaaaccgtcg ccggttgaca
    11881 tcgcctggat gcgcgatgcc ttccgcgacc tgctgcgcat ccgcgccagc acgccactgc
    11941 tgcgcctggg cagcgcggac gacgtgcagc gcaggctcgc gttccgcaat gtcgggccga
    12001 aacaggatcc gctggtggtg gtgggtcatc tggatggcga cggcatgccc ggcgcctatc
    12061 gggaggtgct gtacctgctc aacgtgtcgc cggagacgcg cacgctggcg ctgcccgaag
    12121 aagtgggcaa ggcctatgca ttgcacccgg tgcaagccgc ggatggcgcg gcagacgcgc
    12181 gcgcgaagca ggcacgttat gcagcgaagg atggaacgtt cacggtgccg ggccgcacgg
    12241 cggtggtgtt cgtggtcgaa acgaaaaagg acaggcaatg aaggcactga aattcggaat
    12301 cctgctggcc ctggccttgc ccggcttcgt gcatgcggaa caggtggcgc aggcgacatc
    12361 gccggatggc cgcatcgtgg tgcagctcga cctcaacagc gagggccggt tggcctatcg
    12421 cgtgctgcgc gacggcaagc cggtgatcgc cgattcgcgg ctcggcttca tcctgcgcaa
    12481 cgaccgccag ctgctgcgca acctgcagtt ggacacgcag tcgtccagca gtttcgacga
    12541 gacctgggaa caaccctggg gcgagcgacg cttcgtgcgc aaccactaca acgaactgcg
    12601 tgccgacttc gccgagaagg atcgcgacca ccggcgattc agcatggtgt tccgggtgtt
    12661 cgacgacggt gtcggcttcc gctacgacgt gccgaagcag cccgggctcg cggaagcgaa
    12721 gatcgtgcag gagctgaccg aattcgcggt cgcccggccg tcgacggcgt ggtggatccc
    12781 cgcgtatcaa tggaaccgcg aggaatacct ctacaaccgc acgccgctgt cgcaggtcgg
    12841 cgtggcgcag acgcctatca ccattcgtgc ggatgatggc ctgcacgtga ccctgcacga
    12901 ggcggcgctg gtcgactacg ccggcatgaa cctggcgaag ggcggggggc aggtgctgca
    12961 cgcgtcgctg acgccgggta gcgggccagc gccggtcgtg cgggcgacgc cgttccagac
    13021 gccgtggcgg acgatccaga tcggcgagcg cgcggccgac ctggtcgaat ccagcctggt
    13081 cctcaacctc aacgagccga acaggctcgg cgatgtcggc tggttcaagc cgcacaagta
    13141 cgtgggggtg tggtggtcgt tgcacctgga caaggaaacg tgggcgacgg gacccaagca
    13201 cggggcgacc acggagaaca cgaagcgcta catcgacttc gccgccgcca atggcttccg
    13261 cggcgtgctg gtggaaggct ggaacgtggg ttgggacggc gactggttcg ccaacggctg
    13321 ggacttcgat ttccgcaagc cgacacccga ctacgacctg cgcggactcg ctgcctatgc
    13381 gaaggcgaag ggcgtgcacc tgatcggcca ccacgagacc ggctgcgcgg tcagtcatta
    13441 cgagcggcag atggacgagg cgttcgcgct gttcgacacg attggcatcg atgcggtgaa
    13501 gaccggctat gtctgcgatg ccgggcagat cgaacgacag gacgtggcga agggcccggt
    13561 gctgcgcgaa tggcatgagg gacagtggat gagcaaccac cacctgcgcg tggtgcagga
    13621 tgcggcccgg catcatgtcg cgatcaacgc gcatgagccg atcaaggaca ccggcctgcg
    13681 tcgcacgtat cccaactgga tttcccgcga aggtgcgcgc gggcaggagt tcaatgcgtg
    13741 gggcgatccg cccaacacgc ccgagcacga agtcaccctg gtgtacacgc gcatgctggc
    13801 tggcccgatg gactacacgc cgggcgtggt cagcctgacc ggcaagaacg ggcaggagat
    13861 cggcagcacg ctggcccggc aactggcctt gtacgtggcg ctgtacagcc cgatccagat
    13921 ggccgccgac ctgccggagc actacgcgca gcatcccgat gcgttccgct tcatcaagga
    13981 cgtggcggtg gactgggacg agagccattt gatcgcgggc gaagtcggcg agtacgccgc
    14041 catcgcgcgc aagcaacgcg gcgacgcggc gtggttcatc ggtgcgatca acgaccggca
    14101 tgcgcgcacc gtgccgttga gactcgattt cctcgacgcc ggaaaacgct atcgcgccga
    14161 gatctaccgc gatggcgaag gcgccgactg gcagggcgat gcgcgtttcc gtttcgtccg
    14221 cgaggaacgc actgtgaggc gcggcgacgt gctggatgca tggctcgcgg gcggtggtgg
    14281 catcgccgtg cgactggtcc cgctcgcgcg ctgatccgca acgcttccag cgttgctgtt
    14341 ccatggcgct gggtattgtt gcgctgccac aaatcggcat cgattgatgc cgattgcgct
    14401 tcccgtgcct gttcgatgca gcgttcctgc atgctgagag caagcgctgg cgcgggtttc
    14461 atggattttt tcgttgcatc gccatgaata cgtatgcagg ttggcgtcgc cgcggatcga
    14521 cgcgactacc atcgcgccgt tcgtgtagtg ccgtcccggc ctgcgcgatg atcggatggc
    14581 gagggaccat ccgacacctc attcattcct ggaggggaag atggtgcagt tgaagcgaaa
    14641 cctgttgagc gtggcattgg ccacggccac gatgatgctg gcggcgcagg cacatgcgca
    14701 gtccgcgcct gccgcggacc aggccggaca agaagcggcg gcgaagaaga aggacgatgc
    14761 aaagaacctc gacaccgtcg tggtgaccgg catccgcggc ggcatcgagc gcgcgatcga
    14821 catcaagaag gaatcgacct cgatcgtcga ggcgatttcc gccgaagaca tcggcaagct
    14881 gccggattcg agcatcgccg agtccatctc gcggctgccg ggcatcgccg cgcagcgcgt
    14941 cgccggtcgc gcgcaggtga tcagcgtgcg cggcctgtcg ccggacttcg ccaccacctt
    15001 gctcaatggc cgcgagatgg tgagcacggg cgacaaccgc agcgtggaat tcgaccagta
    15061 cccgtccgaa ctgatggcag gcgtcaccct ctacaagacg ccggacgccg cgctggtggg
    15121 ccagggcctg tccggcacca tcgacatgcg caccgtgcgc ccgctggact acgacaacgc
    15181 cgtgttcgcg gtcagcggtc gcctgacccg cagttcgctg ggatccgcgg ccaacgccga
    15241 cgcgaacggc aaccgcttca acgtgagtta catcggccag accgcggatc gcaaggtcgg
    15301 cttctcgatc ggctattcgc attccgacac gccgatccag gaaaaccagg tcggcctgta
    15361 cgaaccgtgg caggcgatcg gggacaactg gcgtccgggt gttccggccg gcacgtatta
    15421 ctcggatggc atcaaggcac tgcgccggac cggctacacc aagcgcgacg cggtgatggg
    15481 cacgctcgaa ttccgtccgt ccgacgcatg gatcagcacg ctcgacgttt tccactccga
    15541 ggccaagcag gaagacaccg ccaaccagtt cgaagtgcac atcggcgatt acaacggggg
    15601 ttatggtcgc ctgaacgtgc tgaatccgcg gatcgtcaac ggcaccttcg tcggcggcac
    15661 cgcgcagaac gtgtatccgc tggtccgcgg catgtacaac aaccggaagg acgagatcaa
    15721 cgcgttcggc tggcgcaacg agttcttcgt cggccgcgcc aagctggtcg ccgacgtgag
    15781 ctggtccaag tccgatcgcg acgagttgaa cctggaaaac aacctgcagc tcgcgcccgc
    15841 gccccaactg gactcggtgg tgctcgactt cggcggcggt ggtttcccgc agctggatcc
    15901 gggccgggac tattccaacc cggccacgct gttcctgcgc aataccatct atggttccgg
    15961 ctatggcaag accccgaagg tcgcggatga gctcaagggc ttcaaggtct atgcctccct
    16021 cccggcaccg gaagccgcgg ccaacttctt ctccgatatc gacatcgggc tcaactacgc
    16081 cgatcgcgaa aagcagaagt ggcagccgga aggcaacatc aacgtgggtg cccagggcga
    16141 caccacgatc ggttcggaat tccagtacgg cctggtggac ctcgggtttg ccggcgtcgg
    16201 cctgatcccg tcgtgggacg tgccgggcgt ggtcgccaag tacatgacct tcaatcccac
    16261 cgacgatacc gccgcctacc tgatcccgaa ggcctggtcg gtctccgaga cgatcaccac
    16321 cggctacatc aagggcaaca tcgacaccac ctggggttcg gtgggcgtgc gcggcaacat
    16381 cggcgtgcag gtccagcaca ccgaccagtc ctccgcgtcg cgcgtgtggg acagctcgcg
    16441 cccggcgggc agcgagatcc gcccgtacga gcttggcgac aagtacaccg acgtgctgcc
    16501 tagcatgaac ctggcgttct cgttcgagaa cgaccagacc ctgcgtgttg cgctggcgcg
    16561 ccaggtggcg cgtgcgcgca tcgaccagat gcgtgcgtcg ctggagttcg gcgtcaacac
    16621 cgccaccggc gagccgggtg cgggcggcgg caatccgctg ctgcagccgt ggcgcgcgaa
    16681 cgcgttcgac atctcctacg agaagtactt cggcaccaag gcctacgtgg ccgccgcgta
    16741 cttctacaag gacctgcgca gctacatctt cacccagggc cgcgatggct acgacttcac
    16801 cgacctgctg gtcggttacg tcccgccgcc gggctcggca ccggtcaaga acatcggccg
    16861 gatgaccgcg ccgttcaacg gcaagggcgg catgctgcaa ggcctcgagt tgtccgcgtc
    16921 gctcccgctc gacatgctgt gggagccgat gcgcggcttc ggcatcgtgg ccagcgccag
    16981 cttcaccgac agcagcatcg agatcctggc cccggagaac gcgagcagcg tcggcaacgg
    17041 ccccattgcg ttgcctggcc tgtccaagcg ggtctacaac ctgaccgcgt actacgagcg
    17101 caacggtttc gagttccgcg tcaacaaccg ccgccgttcg gacttcatcg gcgagatcgg
    17161 caacttcgcg ggtgagcgtt cgctgcgcta cgtggtgggc gagaacatca ccgatgcgca
    17221 ggtcagctac agcttcggcg aaggcagcag cctgaagggc ctgagcctgc tgttgcaggc
    17281 gagcaacctg ggcaacgaga cctaccagac gtacgccggc agcaaggacc gtccgctcga
    17341 gaacatcgag tggggccgca cgctcctgct gggtgccagc tacaagttct gattgccctc
    17401 cctcccgggt ggcaaccggc ccccgcctcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn gcccagtcgc cggccttcac tgcatccgcc ggcaccatcc acgaaccgcc
    17581 gacgcaggcc acgttcgcca aggccaggta ggcgggtgcc ttggccgcgt cgatgccgcc
    17641 ggtcgggcag aagcgcgcca tcggcagcgg tccgccgaaa cctttcaacg cgccgatgcc
    17701 gccactcgat tccgccggga agaacttgaa gcgccgccag ccgtgctcca tcccgcgcat
    17761 cagttcgctg gcggtggcga tgccggggat cagcggggtg cggctggctt tcgccgcatc
    17821 gaacagcgta tcggtggcgc ccggcgagat cgcgaagcgc gcgccggcgt cggcgacatt
    17881 cgccaggtcg gtggcggaca acaccgtgcc ggcaccgacg caggcatgcg gcagttcgcg
    17941 cgcgaccagg gcgatcgcat cgagtgcgca ggcggtgcgc agggtgatct ccagcaccgg
    18001 caggccgttg tcggcgagtg cgcggcacag cggcaacgcg tcctcgatgc ggtcgatggc
    18061 gagcaccggg atcaccgggg ccaggccgaa cacggcatcg accagttcgc tatggcgggc
    18121 ggtatcgaac atcgggatca gaactccttg gcgcgcaggg cggtggccgc acccagcagg
    18181 ccgggatggg ggtgggtgat gacgtggatc ggcaccgatt ccagcagcgc ggccatccgg
    18241 cccttggcca ggaagcgctc gcggaacgcg ctggcgcgca ggaacgggat gaagcgcggg
    18301 acgatgccgc cggcgatcac caccgtgcgg gcaccgtgga tcagcgcgca gtcgccggcc
    18361 acgctgccga gcaccgagca gaagcgggcc agcgtgcgcc gagcggcctg gtcatgcccc
    18421 tccagcgcgg cggcgacgat ctccgcaggt tcgcgcagtg ccggcttgag cagggaagtt
    18481 tcacccggaa gcagggacgg tcccggcagc accgcgctgg cgccgcgcag gaccacgtcg
    18541 atttcggaca atcccttgcc gcacagcagg cgttcggtcg acgtgcggcc gtgctgtgcg
    18601 gtgagccagg cggagatcgc gcgttcctcg tcgccgaccg gggagaaccc gacatggcca
    18661 ccctcggttt cgaccgcatg ccagccatgc tcgcgtgagc cgacgatcaa ggcgacgccc
    18721 aggccagtgc ccgggccgag cacgctgatc ggcccgcgca gcggcgcagc gggatcgccg
    18781 tgcaaggtca cgaggtgctc gcgctgcagc gccgggatcg cccaggcaac ggcgccgaaa
    18841 tcgttgagca tgcgcagctc gtgcaagccc agcgtgcgtt gcaattcgct gcggctgaag
    18901 gaccaggcgc ggttggtcag gcggatctcg tcgttgccga ccgggcaggc gaccgcgagc
    18961 gcggcgcggg ccggcttcgc gctggcgccg gacaggtagt gctcgatcgc gtgctgcatg
    19021 ctcgcgaaat ccgcgttcgg cagcgccctg gattcgagca gttcgatggt cggcgacgac
    19081 aggtcggtca atgcgaagcg tgcattggta ccgccgatgt cgccgaccag cgcgagcgtg
    19141 ttggcggtca tgccggcatc ccgtcgtctt cgtccaggac gtnnnnnnnn nnnnnnnnnn
    19201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19261 nnnnnnnnnn nnnnnnnnnn nnttcatcca ggaacagcgc gcacgcgccc tgttcggccg
    19321 agccgacctg cttgcgcatc atcgcgaaca gctcgcggcc gtggccgaca tggttgccgg
    19381 ccagcgcggg gcgcacggcc tcgcgcgcgg cccaggcgcc ggcatcgacc agcgcctcca
    19441 tcgtgccggc atcaccgtcg atgcggacca ggtcgccgtc gcgcagcttc gccagcggac
    19501 cggcatcggc ggcttccggg gtgacgtgga tcgccgccaa taccttgccc gacgcgccgg
    19561 acatgcggcc gtcggtgagc agggcaaccc gctggccacg atcctgcagg gcggccagcg
    19621 tgggcgtgag cttgtgcagt tcgggcatcc cgttcgcgcg cggtccctgg ccgcgcacga
    19681 ccgccacgaa gtcaccggcg aatccttcat tccgatcgag tgcaccgacc ttgaacgcgg
    19741 ccaacagcgc gtcctgcgat tcgaagatgc gcgccggcgc ttcgatccgg cgcagttccg
    19801 gtgcgactgc ggagatcttc acgaccgcgc ggcccaggtt gccctgcagg cagcgcaggc
    19861 cgccttcgct gtcgaacggc gcggccacgc cgcgcaccac gttctcgtcc agcgacgtgg
    19921 ccggcgcagt gcgccagcgc aggctgagat cgtccagcca cggttgcgtc gcctgctggc
    19981 gcaggtcgcc gccatgcacg caggcgatgt cgccgtgcag caggccggca tccagcagct
    20041 cgttgatgac caggcccagc ccaccagccg cctcgaaatg gttcacgtcg gcggagccgt
    20101 tcggatacac gcgcgccagc agcggcgtgg cccgcgagag ttcgtccagg tcggtccagt
    20161 cgatgagcag gccggccgcg cgcgccatcg cgacgaggtg cagcgcatgg ttggtggaac
    20221 caccggtggc tgccagcccg accatcgcgt tgacgatcgc cttctcgtcg atggtgcggc
    20281 cgatcgggcg atagtcgttg cccagcgcgg tgatgcgcaa cgcctgctcg gtggcggcca
    20341 cggtcagcgc gtcgcgcagc ggcgtattcg gcggcacgaa ggcgctgccc ggcatgtgca
    20401 ggcccatcac ttccatcagc atctggttgg aattggcagt gccgtagaag gtgcaggtgc
    20461 ccggcgagtg gtaggaggcc gcctcggctt ccagcaattc gtcgcgcgtg gcctggccca
    20521 gcgcgtaccg ctggcgcacc tcggccttct gcttgttggg gatgccggac ggcatcggcc
    20581 cggcgggcac gaagatcacc gggaggtggc cgtagctcag cgccccgatc agcaggccgg
    20641 gcacgatctt gtcgcacacg cccaggcaca gcaccgcgtc gaacatgtcg tgcgacagcg
    20701 agatcgcggt ggccatggca atggcatcgc gcgagaacag cgacagctcc atgccggtgc
    20761 ggccctgggt cacgccgtcg cacatcgccg gcacgccgcc ggcgacctgt gcgctgccac
    20821 cggcattgcg cg
//
LOCUS       DAOOCX010000037        20546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2273_length_20546_cov_8.484701, whole genome shotgun sequence.
ACCESSION   DAOOCX010000037 DAOOCX010000000
VERSION     DAOOCX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 20546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20546
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2273_length_20546_cov_8.484701"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PK399_05285"
     CDS             complement(<1..318)
                     /locus_tag="PK399_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW12565.1"
                     /translation="MHRDPHWIDGKPVPPANGQWLDVFDPATRKVASQVADGDARDVD
                     AAVQAAQAAFPAWSSLPNSVRARWMERLADALEARNDEFARAEAIDAGKPFALARDIE
                     IPRA"
     gene            complement(376..1161)
                     /locus_tag="PK399_05290"
     CDS             complement(376..1161)
                     /locus_tag="PK399_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW12566.1"
                     /translation="MDLNLSGKHALVCGGSEGIGRAAAIELAALGADVTLLARRDDAL
                     RDAVAALPARATQCHGHLVADVSQATALADAVAALIAKKPVHILVNNTGGPPGGPAHA
                     AAIDAYLDAFNKHLVANHTLLQAVLPGMRGARWGRIVNVISTSVYEPIPNLGVSNTIR
                     GAVASWAKTLSRELGGDGITVNNVLPGYTRTQRLDQILADRSTATGKSRDDIANAMLA
                     SVPVGRFAEAGEIGGVIAFLCTHAAAYVNGQSLAVDGGRMQSI"
     gene            complement(1175..1795)
                     /locus_tag="PK399_05295"
     CDS             complement(1175..1795)
                     /locus_tag="PK399_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HPW12567.1"
                     /translation="MYQPRAFVGTDPAQLDALFAADPFVTLLTNDEAGAPFASHVPVL
                     YHRDGDRIVVEGHWAKPNPQARHAGDALLIVHGPHAYVSPGWYPDKHEAGRVPTWNYA
                     VAHLRGRLETFDDAPSLTDLVARLSRHFEASIGSDWEFDATDARQHALLRGIVGFRFM
                     PDRIDIKLKLSQNHPMANRQAVIDALSSQQADDARAVAALMRTTLP"
     gene            complement(1801..2109)
                     /locus_tag="PK399_05300"
     CDS             complement(1801..2109)
                     /locus_tag="PK399_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12568.1"
                     /translation="MNDATRDNWWLASLGNTLVWARLREREAGTAEVFDSDGNTLAYD
                     SLDTARAALMDAEFVEYDGLDEYDALERGFSLHEVSPPQARDDEALRGRMIQQLGRRA
                     "
     gene            complement(2106..2423)
                     /locus_tag="PK399_05305"
     CDS             complement(2106..2423)
                     /locus_tag="PK399_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12569.1"
                     /translation="MDADFVLFLALTAFAIAIAGATAFVIFWPLALVHVRDRHPQITA
                     RLGEGAFLKPKALWWLLRGDYYAAGDRNLSGLATPSRISLLVILGGIAMGGVLWIIGQ
                     AMA"
     gene            complement(2428..3828)
                     /gene="asnS"
                     /locus_tag="PK399_05310"
     CDS             complement(2428..3828)
                     /gene="asnS"
                     /locus_tag="PK399_05310"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPW12570.1"
                     /translation="MTTMSVAQALAGRVPEGGQVTVRGWVRTRRDSKAGLSFVNVSDG
                     SCFAPIQVVAPDTLPNYESEVKHLTTSCAVICTGTLVKSQGQGQSYEIQASSIEVTGW
                     VEDPLTYPMQPKQHSLEYLREFAHLRPRTNLFGAVTRIRHCLSQAVHRFFHERQFYWI
                     STPIITTSDAEGAGQMFRVSTLDMANLPRDGKGNVDFSRDFFGKETFLTVSGQLNVEA
                     YCLALSKVYTFGPTFRAENSNTTRHLAEFWMIEPEIAFADLNDDANLAEDFLKYLFRA
                     VLDERGDDLAFIAERVDKTAITRLESFINAPFERIDYSDAITLLQQSGKKFDFPVEWG
                     LDLQTEHERWLTEEHVGRPVVVMNYPEQIKAFYMRLNDDGRTVAAMDVLAPGIGEIIG
                     GSQREERLDILDARMGQFGLDPAHYGWYRDFRRYGTVPHAGFGLGFERLVVYVCGLAN
                     IRDAIAYPRAPGTAEF"
     gene            3893..4234
                     /locus_tag="PK399_05315"
     CDS             3893..4234
                     /locus_tag="PK399_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HPW12571.1"
                     /translation="MAITLTESARARIRGYLDADPAALGLRFGVSRTGCSGWGYKVDM
                     ARSLAPGDTVFDADGIGIHVDADSLPLVDGTVIDFIKQGLNEQFVFRNPNVAGECGCG
                     ESFTTDADKAA"
     gene            4389..4814
                     /gene="rpsF"
                     /locus_tag="PK399_05320"
     CDS             4389..4814
                     /gene="rpsF"
                     /locus_tag="PK399_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPW12572.1"
                     /translation="MRHYEIVFLVHPDQSEQVPAMIERYKASIEAGNGTIHRLEDWGR
                     RQLAYPIQNLVKAHYVLMNVEAEQPVLDELVSGFRFNDAVLRHLVMRRDDAVTEQSLI
                     MKNKDEKGDKPERGDRRRRDDESDSAVGTGDTESAAEAA"
     gene            4827..5057
                     /gene="rpsR"
                     /locus_tag="PK399_05325"
     CDS             4827..5057
                     /gene="rpsR"
                     /locus_tag="PK399_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPW12573.1"
                     /translation="MSKFFRRRKFCKFTAEGVTEIDYKDLNTLRQYLTETGKIVPSRI
                     TGTKSKYQRQLQSAVKRARFLALIPYTDNHNV"
     gene            5262..5717
                     /gene="rplI"
                     /locus_tag="PK399_05330"
     CDS             5262..5717
                     /gene="rplI"
                     /locus_tag="PK399_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006376492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPW12574.1"
                     /translation="MQLILLQKVVNLGNLGDKVSVKPGYGRNFLVPQGKAVPATAANL
                     AEFEAKRADYEAKAQANLDGATARQAKLAEASVTISANASTEGKLYGSVGPREIAEAA
                     TKQLGVTVGKSEVVMGEGPIRHTGEFEVMVHLHADVESSLKVIVVPDAA"
     gene            5954..7366
                     /locus_tag="PK399_05335"
     CDS             5954..7366
                     /locus_tag="PK399_05335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPW12575.1"
                     /translation="MSARPGSRPDASFHTRNDAMIEHLRVPPQSVEAEQAVLGGLMLD
                     PNAFDRVADQLVEDDFYRRDHQLIYRAIRELVEKNQPCDAVTVGDWFDSQGKAEQVAG
                     GAYLVELAASTPSAANIKAYADIVRDKAIQRKLIDVGTGIVNDGFQPEGRDSSELLAK
                     AEQEVFAIAEGHGRGRQDFTPVRSALIEAFDVLQKRAENGGSVTGLPTGYTEFDEMTA
                     GLQPTDLLILAARPAMGKTTFALNIAEYGALKSKKAVAVFSMEMSASQLALRLISSLG
                     RVNATRLRTGQLEDEDWSRVTGAIAQMNGVKIFIDDTPALSPDVLRAKSRRLKREHDL
                     GLIVIDYLQLMAVPGNSENRATEISEISRSLKGLAKELNVPVIALSQLNRSLETRTDK
                     RPVMADLRESGAIEQDADVIVFIYRDEYYNKENSPDKGLAEIIIGKQRNGPTGSFKLK
                     FFGEYTRFDNLAHDMVGSFE"
     gene            7509..7634
                     /locus_tag="PK399_05340"
     CDS             7509..7634
                     /locus_tag="PK399_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12576.1"
                     /translation="MLRKQNLLTHGTLEARQPAQGFLKHARWPQKVKGQVRSMVE"
     gene            7639..8778
                     /gene="alr"
                     /locus_tag="PK399_05345"
     CDS             7639..8778
                     /gene="alr"
                     /locus_tag="PK399_05345"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPW12577.1"
                     /translation="MSEIASQRPSRIVVDLDAIGHNLQAIRGHVGVPVMGIVKANAYG
                     HGLVPVARHLQARGVDQLGVAFVEEGVALRRAGISIPILVLGGIFGPQVAQFLEHDLE
                     ITVSSLDKLRQVEATARAMGRKATIHLKIDTGMERIGVHSYSAGPFIEAAAASTWCIL
                     KGIYSHLACADDPASPMTALQLERFNQACAHFERIGAPMPLRHLANSGGVLHFPETWL
                     DIVRPGIALYGVLPDPASQPTIPLRPALSLVSRVVFFKVVKAGNPVSYGATWASAVDT
                     RVVTVPVGYGDGYPRALSSRGQVLLHGQPHPLVGRVCMDQFMVDIGPEGSAYNEDEVV
                     LIGRQGEAAISTEALAQRAGTIPYEILTGLNERIPREYVGGASSR"
     gene            8830..10332
                     /locus_tag="PK399_05350"
     CDS             8830..10332
                     /locus_tag="PK399_05350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPW12578.1"
                     /translation="MATSKPRAERPGEGQGRRLKREPMSRVDTAWLRMERPTNPMMIT
                     GVMMFAEPMTRAQLKRVIQQRFLAYARFRQKPVDGATGAQWVEDADFDLDWHVRLSGL
                     PGKNNPAAEKKALERFVSQLASSPLDPTKPLWQFHLIERYQGGSAIVARIHHCYADGI
                     ALVQVLLSLTDTSRESSQASRLDKAWLKQDAAPVARRVGAVDKYMKLGGKVLEQGMAM
                     YRDPTLAGLIAKEGGELARELVTALALPDDPPSLLRGRLGVSKRVAWAEPLDLEEVKA
                     VGRACDCTVNDVLMAAAAGALRGYMLDRGEHLEGMTLRATVPVNLRPLEHAKKLGNHF
                     GLVFLELPVGEDNPLRRLERVAECMNQLKSSRQAIVAYGLLAALGLAPPQLQEVALEM
                     FSRKASAVATNVPGPQQPLYLAGCMLRDIMFWVPQTGSIGLGLSILSYRGQVHFGLVT
                     DARLIPDPDAVVRRFGAEFEKLLYLSLMGGWEHPLDADGATALLEEPTPA"
     gene            10435..11112
                     /locus_tag="PK399_05355"
     CDS             10435..11112
                     /locus_tag="PK399_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW12579.1"
                     /translation="MNTHTLKVAALAATLAAFTSGCATYTGQTNDPNDPNRTRNGALI
                     GAAIGAAAGLLSGGNAVERRQRAMVGVGVGALAGGAVGAYQDRQEAELRRATAGTGVD
                     VTRDGDVIKLNLPDGVTFDFARYDIKPQFYPALNTIANTLREYNQTIVEVSGHTDSVG
                     SDAANQALSERRANAVSGYLIGQGLQRERFEVVGMGERYPIASNDTDQGRALNRRVEI
                     RLLPLQG"
     gene            11224..11578
                     /gene="ssrA"
                     /locus_tag="PK399_05360"
     tmRNA           11224..11578
                     /gene="ssrA"
                     /locus_tag="PK399_05360"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            11662..13884
                     /locus_tag="PK399_05365"
     CDS             11662..13884
                     /locus_tag="PK399_05365"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HPW12580.1"
                     /translation="MATLHPSPDSDALPALRRMRYGYPFAILVLVVSVLFVLVAWDHA
                     RQRELRLASQQFRGIAGQQASLVQMHLNNIEVTLRGGASLFAAVDQPTPEQWRGYVDG
                     LRVPSRFPYVLGLGYAVYVDGHGLTRLQEAWREQGRGLLEIRPRGHRERYGPILFLEP
                     HSPGNRAALGFDMYAEATRRAAMRAAMESGRSQLSGRVVLVQDAAQPEYSLVLYMPVY
                     AGLEATESPQLRRDSMRGWVYAPFRIGAMLRAALPPAQTRTRLRVVDTTDGGHVVMHQ
                     DAGIETTRHAFTHSLTMQFYGRRWRFDFFSGPTASAAPQLAALDKLLFAGLAASLLLF
                     AMTWMLASTEARARRIAADMTQSYRRSEQRFRNAMQYSAIGKALLDSNGAIVESNPAF
                     GRIFGRSDSTLRGHSLNEFLDEGATQPLVTSQMQAFDESGGVIRLTRTLRRGDGEVRH
                     VHLTFAPVPNEPGIDVARLVQVEDVSERVRAEAAVQALNRTLEARVAARTRELSEANR
                     ELESFAYSVSHDLRAPLRGIEGFSRLLGERHAHGLDATGRDYLERVRRGTARMGELID
                     ALLKLARIGRAEPEVAEVDLSALAAEVAAGLADADPGRNIDLRIEPGMRTRGDRTLLQ
                     NLLENLLGNAWKFTRGRDDARVEFCREPGGDDEAWFVIRDNGAGFDPDYANKLFKPFQ
                     RLHSQDEFPGHGIGLASVKRIVERHGGEIQAEGSPGQGATFRFTLGTSTEVRMDDGTR
                     "
     gene            complement(13998..14492)
                     /gene="smpB"
                     /locus_tag="PK399_05370"
     CDS             complement(13998..14492)
                     /gene="smpB"
                     /locus_tag="PK399_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPW12581.1"
                     /translation="MNKKTGKDKPNGGGTIALNKRARHEYHFERTFEAGLSLQGWELK
                     SIRAGRANLTDGYAVVLQGELFLVGAQITPLIQASTHVVANDRRSRKLLLHRHEIDEL
                     IGKVQRDGYTLIPTALYWKGNKVKMELALARGKQSHDKREASKERDWNREKQRVMRQH
                     NKDA"
     gene            14550..14981
                     /locus_tag="PK399_05375"
     CDS             14550..14981
                     /locus_tag="PK399_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HPW12582.1"
                     /translation="MPVILRSALVEHSAARMFALVNDVAAYSRRFDWCREAQVLEQGD
                     DRLVARLDLGLGGFHTWFTTENTLSPPHHIDMALRDGPFRRLDGRWQFHALDESACKV
                     SLRLEFEPQNRLLGPALSLGMQGLADRMVDDFVREADKGGA"
     gene            14981..15253
                     /locus_tag="PK399_05380"
     CDS             14981..15253
                     /locus_tag="PK399_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HPW12583.1"
                     /translation="MHIDVLRAWPHRHESRAVEVGEGATVATVLAASGWGMDAEFTAV
                     AVFGVAASSDTVLHEGDRVELLRPLQLDPKQARRLRAELRPLKSAR"
     gene            complement(15264..15656)
                     /gene="bamE"
                     /locus_tag="PK399_05385"
     CDS             complement(15264..15656)
                     /gene="bamE"
                     /locus_tag="PK399_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HPW12584.1"
                     /translation="MRKLLAIVLVVLFASGCGVLYRQPIYQGNLLDKTAVDQLQQGMD
                     KRQVMTLLGTPSVQDPFHHDRWDYVASRRTGRSGPTEVKNMTLWFENGSLSKWEGEYF
                     PEEDSELAKTATKSFGPNLQKDKKKGGR"
     gene            15798..16199
                     /gene="fur"
                     /locus_tag="PK399_05390"
     CDS             15798..16199
                     /gene="fur"
                     /locus_tag="PK399_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HPW12585.1"
                     /translation="MESQDLRKAGLKVTHPRMRILELLEQTKPRHMTAEDIYRQLLGD
                     GEEIGLATIYRVLTQFESAGLVLKHNFEGGHAVYELDRGEHHDHMVDVDTGKVVEFHS
                     DEIEELQRQIAAAHGYEIEDHALVLYVRKKR"
     gene            complement(16260..17918)
                     /gene="recN"
                     /locus_tag="PK399_05395"
     CDS             complement(16260..17918)
                     /gene="recN"
                     /locus_tag="PK399_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPW12586.1"
                     /translation="MLSRLSIRDFAVVAHAELAFGRGLTVVSGETGAGKSLLVDALGF
                     LGGARADAGAVRHGAQRAELAAEFALDDCPAAMAWLRDAELDDEDACQLRRTLRADGG
                     SRAWINGRPVPLAQLAELAGLLVEIHGQHEQQALLSRAHQTRLLDAHARHPGLLQAVR
                     STALQWKGLQEERSALLARGDVGERQSWLRHQLQELDAETLAPEALAELDASHRRQAH
                     AASLIAACDHALEQLGGDHAVAAQLHHLRGSLQREMTHEPRLGDVDALLDSAAIQLDE
                     AFAMLERIRDDLDLDPDALDALERRLARVQDLARKHRVAPDGLAAHRESLARELDTLA
                     DADERLQRIERDITQAHAAWTQAAAELTASRRKAGDALAQSVATLIAELGMQGGQFEV
                     LLELDDSDTPDPQGAERADFLVSANAGQPPRPLRKVASGGELSRISLAIEVATLGTDA
                     VATMVFDEVDSGIGGAVAAAVGAKLRALGAPRQVLCVTHQPQVAAAGHSHYRVSKAAA
                     EGMTQSSVVVLDAEGRIDEIARMLGGTQVSDEARAAARKLLDGF"
     gene            17996..19057
                     /gene="hrcA"
                     /locus_tag="PK399_05400"
     CDS             17996..19057
                     /gene="hrcA"
                     /locus_tag="PK399_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPW12587.1"
                     /translation="MSRRPSDIASDPRARQLLRTLVARHVRDGEPVGSKTLARHAGLD
                     VSPATIRNILATMEEAGLLVAPHSSAGRIPTAQGYRLFVDSLLQVKPLGDVELQRLRS
                     ELPVGAGTQALLGNTSELLSAMTRFAGMVSVPKREGFAFRQIEFVALDPQRVLAIVVF
                     ADGEVQNRVVQVRRAFDPAELEQTANYLNAHFAGQPLAQIRAALLRDLRDARSEMERL
                     LASSLDLAEQAFAPGREDAPFHGMLVAGQTRLMGLQDLNDLDRLRALFEAFAEKRELL
                     QLLERTAKAPGMRVFIGEETGIAPLEGMSLVTAPYGRDGRVLGVLGVIGPSRMAYERV
                     IPVVEATAAILGAALDAPS"
     gene            19163..19657
                     /gene="grpE"
                     /locus_tag="PK399_05405"
     CDS             19163..19657
                     /gene="grpE"
                     /locus_tag="PK399_05405"
                     /inference="COORDINATES: protein motif:HMM:NF010745.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPW12588.1"
                     /translation="MQDPIQDVNDAAGAMPQDDELEALRGELEQLKAQSLLERADLEN
                     QRKRIAREIDSARKFANERLLGDLLPVFDSLDAGLTAAGSESSPLRDGLEMTYRQLLK
                     VAGENGLSVLDPTGEAFNPEHHQAISQMQAAGVAADHVAQVFQKGYLLNGRLLRPALV
                     VVAA"
     gene            19769..>20546
                     /locus_tag="PK399_05410"
     CDS             19769..>20546
                     /locus_tag="PK399_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004085852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPW12589.1"
                     /translation="MGKIIGIDLGTTNSCVAIMEGGKAKVIENSEGDRTTPSVVAWSK
                     DNEVLVGASAKRQAVTNPKNTFYAVKRLIGRKFTDAEVQKDLGLVPYAITAHDNGDAW
                     VSLVDGKKLAPQEVSAKVLEKMKKTAEAYLGEPVTEAVITVPAYFNDSQRQATKDAGR
                     IAGLDVKRIINEPTAAALAYGMDKGEKTDRKIAVYDLGGGTFDVSIIEIANVDGEKQF
                     EVLATNGDTFLGGEDFDKRVIDYLVEEFQKEQGIDLRKDPL"
BASE COUNT         3416 a         6785 c         7007 g         3338 t
ORIGIN      
        1 cgcgcgcggg atctcgatat cgcgggcgag ggcgaatggc ttgccggcgt cgatggcctc
       61 ggcacgcgcg aattcgtcgt tgcgcgcttc gagcgcgtcc gccagccgtt ccatccagcg
      121 cgcacggacg ctgttcggaa gcgacgacca tgcggggaat gcggcctgtg cggcctgcac
      181 ggcggcgtct acatcgcggg catcgccatc ggcgacctgc gaggcgacct tgcgcgtggc
      241 ggggtcgaac acatccagcc actgcccgtt ggcgggcggg acgggcttgc catcgatcca
      301 gtgcgggtcg cgatgcatgc ggctagttta gagcggttta gagcgaatgc tcctcgacgc
      361 cggagcggcg cttgctcaga tcgactgcat ccgtccgcca tcgaccgcga gcgattgccc
      421 gttgacgtag gccgcagcgt gcgtgcacag gaaggcaatc acgccaccga tctcgccggc
      481 ctcggcgaaa cgcccgaccg ggacgctggc cagcatggcg ttggcgatgt cgtcccgcga
      541 cttgccggtc gcagtgctgc ggtcggccag gatctggtcc aaccgctggg tgcgggtata
      601 gccgggcagg acgttgttga cggtgatgcc gtcgccgccg agttcgcgcg acagggtctt
      661 ggcccagctc gccaccgcgc cgcgtatggt gttggagacg ccgaggttcg ggatcggctc
      721 gtacaccgac gtggagatga cgttgacgat gcggccccag cgcgcgccgc gcatgcccgg
      781 caggaccgcc tgcagcaacg tgtggttggc gaccaagtgc ttgttgaagg catcgaggta
      841 ggcgtcgatg gcggccgcat gcgcggggcc gcccggcgga ccaccggtgt tgttgaccag
      901 gatgtggacg ggcttcttcg cgatgagcgc agcgacggca tcggccagcg ccgtcgcctg
      961 cgacacgtcg gcaacgagat ggccatggca ttgtgtcgcc ctggccggca aggcggctac
     1021 ggcatcgcgc agcgcgtcgt cgcgacgggc gagcagggtg acatcggcgc cgagcgcggc
     1081 cagctcgatc gccgcggcgc ggccgatgcc ttcggaaccg ccgcagacca gggcgtgctt
     1141 gccggacagg ttcaggtcca tcgtatgggc ttcctcaggg cagggtggtg cgcatcagcg
     1201 cggcgacggc gcgggcgtcg tcagcttgtt gcgacgacag cgcgtcgatc accgcctgcc
     1261 ggttcgccat gggatggttc tggctcaact tcaacttgat gtcgatccga tccggcatga
     1321 agcggaagcc gacgatgccg cgcagcaagg catgctgccg cgcatcggtg gcatcgaatt
     1381 cccagtcgct gccgatgctg gcctcgaaat gccggctcag gcgcgcgacc aggtcggtga
     1441 gcgagggcgc gtcgtcgaac gtctccaggc ggccgcgcag gtgcgccacg gcgtagttcc
     1501 aggtcggcac gcggcctgct tcgtgcttgt ccggatacca gcccggcgag acgtaggcat
     1561 gcggaccgtg cacgatcagg agggcatcgc cggcatgccg ggcctgcggg ttcggtttcg
     1621 cccagtggcc ttcgacaacg atccgatcgc cgtcgcggtg gtacagcacc ggcacgtgcg
     1681 aggcgaacgg cgcaccggcc tcgtcgttgg tgagcagggt gacgaacgga tccgcggcga
     1741 acagggcgtc gagctgcgcc gggtcggtgc cgacgaaggc gcgcggctgg tacatcgcgc
     1801 tcaggcgcgg cggccgagtt gctggatcat ccgtccgcgc aaggcctcgt cgtcgcgtgc
     1861 ctgcggtggc gacacctcat gcagcgagaa gccgcgctcc agcgcgtcgt attcgtccag
     1921 gccgtcgtat tcgacgaact cggcatccat cagggccgcg cgtgcggtat ccaggctgtc
     1981 gtaggccagc gtgttgccgt cgctgtcgaa gacctctgcg gtgccggcct cgcgttcgcg
     2041 caggcgcgcc cagacgagcg tattgcccag cgaggccagc caccagttgt cgcgagttgc
     2101 gtcgttcatg ccatcgcctg cccgatgatc cacagcacgc cgcccatcgc gatgccgccc
     2161 aggatcacca gcagcgagat ccgcgacggc gtggccagcc cgctcaggtt gcggtcgccc
     2221 gccgcgtaat agtcgccgcg cagcagccac cacagcgcct tcggcttgag gaaggcgcct
     2281 tcgccgagcc gtgcggtgat ctgcggatgg cggtcgcgga cgtgcaccag cgcgagcggc
     2341 cagaagatca cgaacgcggt ggccccggcg atcgcgatgg cgaaggcggt cagcgccagg
     2401 aacaggacga aatcggcgtc catcggatca gaactccgcc gtgcccggcg cgcgcgggta
     2461 ggcgatcgca tcgcggatgt tggccagccc gcagacatag accaccaggc gctcgaagcc
     2521 caggccgaag ccggcgtgcg gcacggtgcc gtagcgacgg aaatcgcggt accagccgta
     2581 atgcgccgga tccaggccga actggcccat gcgcgcgtca aggatgtcca ggcgctcttc
     2641 gcgctggctg ccgccgatga tctcgccgat gccgggcgcc agcacgtcca tcgcggcgac
     2701 ggtgcggccg tcgtcgttca ggcgcatgta aaaggccttg atctgctccg ggtaattcat
     2761 gaccacgacc gggcgtccca cgtgttcctc ggtcagccag cgctcgtgct cggtctgcag
     2821 gtccaggccc cactcgaccg ggaagtcgaa cttcttgccg gactgctgca gcagggtgat
     2881 cgcatcgctg tagtcgatgc gctcgaacgg tgcattgatg aagctttcca ggcgggtgat
     2941 ggcggtcttg tcgacgcgct cggcgatgaa agcgaggtcg tcgccgcgct cgtccagcac
     3001 cgcgcggaac aggtacttga ggaagtcctc ggccaggttg gcgtcgtcgt tgaggtcggc
     3061 gaaggcgatt tccggctcga tcatccagaa ctcggccagg tggcgcgtgg tgttgctgtt
     3121 ctcggcgcgg aaggtcgggc cgaaggtgta gaccttgctc agcgccaggc agtaggcctc
     3181 cacgttgagc tggccgctca cggtcaggaa ggtttccttg ccgaagaagt cgcgactgaa
     3241 gtccacgttg cccttgccgt cgcgcggcag gttggccatg tccagcgtcg acacgcggaa
     3301 catctggccg gcgccctcgg cgtccgaggt ggtgatgatc ggcgtgctga tccagtagaa
     3361 ctggcgctcg tggaagaagc ggtgcacggc ctgcgacagg caatggcgga tgcgggtgac
     3421 cgcgccgaac aggttggtgc gcggacgcag gtgggcgaat tcgcgcaggt attccagcga
     3481 gtgctgcttg ggctgcatcg gataggtgag gggatcctcg acccagccgg tgacctcgat
     3541 gctggaggcc tggatctcgt agctctggcc ctggccctga gacttcacca aggtgccggt
     3601 gcagatgacc gcacagctgg tggtgaggtg cttcacctcg gactcgtagt tgggcagggt
     3661 gtccggggcc acgacctgga taggagcgaa gcaggagccg tcactgacgt tgacgaagct
     3721 caggccggcc ttggaatcgc ggcgggtgcg cacccagccg cgcacggtca cctggccacc
     3781 ttccggcacg cgcccggcga gggcctgggc cacggacatc gtcgtcatct tgaaatcctg
     3841 cgttgcggca tcgaaatagg tcgggcagtt taccggagct acaatccccc gcatggccat
     3901 cacccttacc gaatccgccc gcgcccgcat ccgcggctac ctcgacgccg acccggccgc
     3961 gctcggcctg cgtttcgggg tgtcccgcac aggttgttcc ggctggggct acaaggtcga
     4021 catggcgcgc agcctcgcgc ccggggacac cgtattcgac gccgacggca tcggtatcca
     4081 cgtcgacgcg gacagcctgc cgctggtgga cgggaccgtc atcgatttca tcaagcaggg
     4141 cctgaacgag cagttcgtgt tccgtaatcc caacgtggcc ggcgaatgcg gctgcgggga
     4201 atcgttcacg acggatgccg acaaggccgc ctgagccgcc ccggcgcgca ggatttgcgc
     4261 gcaagtcctt gaatctgcca tactttgcgg ctcccgttcg cgggagctct tgcccgaacg
     4321 cgccgtgatc acggtggccg agggcttcgg ccggcatccc gccgcccgat atccacaagg
     4381 aaacgaccat gcgtcattac gaaatcgtgt tcctggtcca cccggaccag agcgagcagg
     4441 tgcccgccat gatcgagcgc tacaaggcct cgatcgaagc cggcaacggc accatccacc
     4501 gcctggaaga ctggggccgc cgccagctgg cgtacccgat ccagaacctg gtgaaggccc
     4561 actacgtgct gatgaacgtg gaagccgagc agcccgtcct cgacgagctg gtctccggtt
     4621 tccgcttcaa cgacgcggtg ctgcgccacc tggtcatgcg tcgcgacgac gcggtgaccg
     4681 agcagtccct gatcatgaag aacaaggacg agaagggcga caagccggag cgcggcgacc
     4741 gccgccgccg tgacgacgag agcgacagtg ccgtcggcac tggcgacacc gaatccgccg
     4801 ccgaagccgc ctaaggaacc gcccacatgt ccaagttctt ccgtcgccgc aagttctgca
     4861 agttcactgc cgaaggcgtg accgagatcg actacaagga tctcaacacc ctgcgccagt
     4921 acctgaccga gaccggcaag atcgtgccga gccgcatcac cggcaccaag tcgaagtacc
     4981 agcgccagtt gcagtcggcc gtcaagcgcg cgcgtttcct ggcgctgatc ccctacaccg
     5041 acaaccacaa cgtctgattg cttcgccagc tcggtctggc gtcgttgccg ggccggttcg
     5101 caaatcctca ttggcttatg ccaactgcgg tttgctcacc ggcccgcgcc tagccagccc
     5161 atcgctggct gtgcaatcga cggtcttcgc ccacaccccg tccattcgga cagcaaccgt
     5221 tgcggcgcga tccatgccgc taacgaatac ggagcactga aatgcaactg atcctcctgc
     5281 agaaagtcgt gaacctcggc aaccttggcg acaaggtcag cgtcaagccg ggctacggcc
     5341 gcaacttcct ggtgccgcag ggcaaggccg tgccggcgac cgccgccaac ctggccgagt
     5401 tcgaagccaa gcgcgccgat tacgaagcca aggcccaggc caacctggac ggcgcgaccg
     5461 cgcgccaggc caagctggcc gaggccagcg tgaccatcag cgccaatgcg tcgaccgaag
     5521 gcaagctgta cggctcggtc ggcccgcgcg aaatcgccga ggccgccacc aagcagttgg
     5581 gcgtgaccgt cggcaagtcg gaagtggtga tgggcgaagg cccgatccgc cacaccggcg
     5641 agttcgaagt gatggtgcac ctgcatgccg acgtcgagtc gagcctgaag gtcatcgtgg
     5701 tgcccgacgc cgcgtaatcc cgttccatcg ggcgatgtcg agacgggtgc cgcaaggcgc
     5761 ccgttttcgt tgggcgtgcc gcccgtcgca cggtcgcggg atctgcgatg cacggctggt
     5821 atcccggcaa ggttatccag aactatccac aggcttatct gcaaggcatc ggccgggctg
     5881 cgactacgat gtcggccttg ccaatgcacc accagccgat gcgcatgcac gcatcgcagg
     5941 gagcaacatc tcgatgagcg cacgaccggg ctcccgtccc gacgccagtt tccatacccg
     6001 caacgacgcc atgatcgagc acctgcgggt gccgccgcag tcggtcgaag ccgaacaggc
     6061 ggtgctgggc ggtttgatgc tggatccgaa cgccttcgat cgggtggccg accaactcgt
     6121 cgaggacgat ttctaccgcc gcgaccacca gttgatctat cgcgcgatcc gcgaactggt
     6181 ggagaagaac cagccttgcg atgcggtgac ggtgggcgac tggttcgact cgcagggcaa
     6241 ggccgagcag gtcgccggtg gcgcctacct ggtcgagctc gccgccagca cgcccagcgc
     6301 ggccaacatc aaggcctacg ccgatatcgt ccgcgacaag gcaatccagc gaaagctgat
     6361 cgatgtcggt accggcatcg tcaacgacgg gttccagccg gagggccgcg attcctccga
     6421 attgctggcc aaggcggaac aggaagtctt cgccattgcc gagggccacg gccgcggccg
     6481 ccaggacttc accccggtgc gcagcgcgct gatcgaggcc ttcgacgtgc tgcagaagcg
     6541 cgccgagaac ggcggctcgg tgaccggcct tcccaccggc tacaccgagt tcgacgagat
     6601 gaccgcgggc ctgcagccca ccgacctgtt gatcctggcc gcgcgtccgg cgatgggcaa
     6661 gaccacgttc gcgctgaaca tcgccgagta cggtgcgttg aagtcgaaga aggcggtggc
     6721 ggtgttctcg atggaaatgt cggcctcgca gctggcgttg cgcctgattt cctcgctggg
     6781 ccgggtcaat gccacgcgcc tgcgcaccgg ccagctggag gacgaggact ggagccgtgt
     6841 caccggcgcg atcgcgcaaa tgaacggggt caagatcttc atcgacgaca ccccggcgct
     6901 gtcgcccgac gtgctccgcg cgaagtcgcg acggctcaag cgcgagcacg acctcggcct
     6961 gatcgtgatc gactacctgc aactgatggc ggtgcccggc aacagcgaga accgcgccac
     7021 cgagatctcc gagatcagcc gctcgctcaa gggcctggcg aaggaactca acgtcccggt
     7081 gatcgcgctg tcgcagctca accgctcgct ggaaacgcgc accgacaagc gcccggtgat
     7141 ggccgacctg cgcgagtccg gcgcgatcga gcaggacgcg gacgtgatcg tcttcatcta
     7201 ccgcgacgag tactacaaca aggaaaattc gccagacaag ggcctggccg agatcatcat
     7261 cggcaagcag cgaaacggcc cgaccggttc gttcaagctg aagttcttcg gcgagtacac
     7321 ccgcttcgac aacttggcgc acgacatggt ggggagcttc gagtaattac ggtcatgcat
     7381 cccggggcgg aaccctcgcg ccggctagcg tgcttcgccg cttagcatcc agctccgatg
     7441 cgcggccgcg cgcccgctcc gcggcccggc ccgccacagc gtggcaggcg ttcgctggtt
     7501 cgcgcagcat gctgcgcaag cagaacctgc tcacccatgg cacgctcgaa gcacgccagc
     7561 cggcgcaagg gttcctcaag cacgctcgct ggccacagaa ggtcaagggc caagtccgat
     7621 ctatggtcga atagcggcat gagcgaaatc gccagccagc gcccgagccg gatcgtcgtc
     7681 gacctcgatg caatcggcca caacctgcag gccatccgcg gccacgtggg cgtgccggtg
     7741 atgggcatcg tcaaggcgaa tgcctacggc catggcctgg tgccggtggc ccgccacctg
     7801 caggcgcgcg gcgtcgacca gttgggcgtc gcgttcgtcg aagagggtgt cgcgctgcgc
     7861 cgtgccggca tcagcatccc gatcctggtg ctcggcggca tcttcgggcc gcaggtcgcg
     7921 cagttcctgg agcatgacct cgagatcacc gtgtcctcgc tcgacaagct gcgccaggtc
     7981 gaggcgacgg cgcgggcgat gggtcgcaag gcgaccatcc acctgaagat cgataccggg
     8041 atggagcgga tcggcgtgca cagctattcg gctgggccgt tcatcgaggc cgcggcggcg
     8101 tcaacctggt gcatcctcaa gggcatctac tcccacctgg cctgcgcgga tgatccggcg
     8161 tcgccgatga cggcgctgca actggagcgc ttcaaccagg catgcgcgca tttcgagcgg
     8221 atcggcgcgc cgatgccgct gcgccacctg gcgaattccg gcggcgtgct gcatttcccg
     8281 gagacctggc tggacatcgt ccgccccggc atcgccctgt acggcgtgct gccggatccg
     8341 gcatcgcagc ccacgattcc gctgcggccg gcgctgtcgc tggtctcgcg ggtggtgttc
     8401 ttcaaggtgg tcaaggccgg caacccggtc agctacggtg ccacctgggc atccgccgtc
     8461 gatacgcgcg tggtcaccgt gccggtcggg tatggcgacg gctatccgcg cgcgctgtcg
     8521 tcgcggggac aggtcttgct gcacggacag ccgcatcccc tggtcggtcg cgtgtgcatg
     8581 gaccagttca tggtcgacat cgggcccgag gggtcggcct acaacgagga cgaggtcgtg
     8641 ttgattggca ggcagggcga ggccgcgatc agcaccgaag cattggcgca acgcgccggc
     8701 acgatcccct acgaaatcct caccggactc aacgagcgca tcccgcgcga atacgtcggc
     8761 ggcgcctcat cgcgttgacc gtctccacac ggcgtgattc actcggccga tgatcctggg
     8821 gaggaccgga tggctacgtc gaaaccgcgc gcggagcgcc ctggcgaagg gcagggcagg
     8881 cgattgaagc gcgagccgat gtcgcgggtc gataccgcct ggctgcggat ggagcggccg
     8941 acgaacccga tgatgatcac tggcgtgatg atgttcgccg agccgatgac ccgggcgcag
     9001 ctgaaacggg tgatccagca gcgcttcctc gcctatgcgc ggttccggca gaagccggtg
     9061 gatggcgcga ccggcgcgca atgggtggaa gacgcggact tcgacctcga ctggcatgtg
     9121 cgcctttccg gcttgccggg caagaacaat cccgccgccg aaaagaaggc attggagcgt
     9181 ttcgtcagcc agttggcgtc cagcccgctg gatcccacca agccgctctg gcagttccac
     9241 ctgatcgagc gctaccaggg cggctccgcg atcgtcgccc gcatccacca ttgctatgcg
     9301 gacggcatcg ccctggtgca ggtcctgctg tcgttgaccg ataccagccg cgagtcctcg
     9361 caggccagcc gcctcgacaa agcgtggctg aagcaggatg ccgcaccggt ggcgcgtcgc
     9421 gtcggcgcgg tcgacaaata catgaagctg ggcggcaagg tgctggagca gggcatggcg
     9481 atgtatcgcg atcccaccct ggccgggctg atcgcgaagg aaggcggtga gctggcgcgc
     9541 gaactggtga ccgcactggc gttgccggac gatccgcctt cgttgctgcg cgggcgcctg
     9601 ggggtgagca agcgggtggc ctgggcggag ccgctggacc tggaggaagt gaaggccgtt
     9661 ggccgcgcct gcgattgcac cgtcaatgac gtcctcatgg cggccgcggc cggggcgctg
     9721 cgcggctaca tgctggatcg cggggaacac ctggaaggca tgaccctgcg tgccaccgtg
     9781 ccggtcaacc tgcggcccct ggaacacgcg aagaagctgg gcaaccactt cggcctggtg
     9841 ttcctggaat tgcccgtcgg cgaggacaat ccattgcgtc ggctggagcg ggttgccgag
     9901 tgcatgaacc agttgaagag ttcaaggcaa gccattgttg catatggact tttggctgcg
     9961 cttggcttgg cacccccgca gttgcaggaa gtcgcgctgg agatgttcag ccgcaaggcc
    10021 agtgcggtgg cgaccaatgt cccgggtccg caacagccgc tgtaccttgc tgggtgcatg
    10081 ctgcgcgaca tcatgttctg ggtgccgcag accggctcga tcgggctggg gctgtcgatc
    10141 ctgagttacc gcggacaggt gcattttggc ctggtgaccg atgcgcgcct gatcccggac
    10201 ccggatgcgg tcgtccggcg cttcggcgcg gagttcgaga agctcctgta cctgtcgctg
    10261 atggggggct gggagcatcc gctggatgcg gacggcgcca ccgcgttgct ggaagaacca
    10321 acgccggctt aacggcgctg tccgtgttca cctgccggct acgttcgccg gcctagcctg
    10381 tggccgcatt caggatttgc ctggacgaca acgcacccac ggagctcgac gcgcatgaat
    10441 acccataccc tcaaagttgc tgcactcgcc gccaccttgg ccgccttcac gtccggctgc
    10501 gccacctaca ccggccagac caacgatccg aacgatccca accgcacgcg caacggtgcc
    10561 ctgatcggtg cggccatcgg cgcggcggcc ggcctgctga gcggtggcaa cgcggtcgag
    10621 cgtcgccagc gcgcgatggt gggcgtgggc gtcggtgcct tggccggtgg tgcagtcggt
    10681 gcctaccagg atcgccagga agccgagctg cgtcgcgcca ctgcgggcac cggcgtggac
    10741 gtgacccgcg atggcgacgt catcaagctg aacctgccgg atggcgtcac cttcgacttc
    10801 gccaggtacg acatcaagcc gcagttctat ccggcgctga ataccatcgc gaacaccctg
    10861 cgcgaataca accagactat cgtggaagtg agtggccaca ccgatagcgt gggatccgat
    10921 gccgccaacc aggcgctgtc cgagcgccgt gcgaatgcgg tatccggata tctcatcggc
    10981 cagggcctcc agcgcgagcg cttcgaagtg gtgggcatgg gcgagcgcta tccgatcgcc
    11041 agcaacgaca ccgaccaggg ccgcgccctg aaccgccgcg tcgagatccg cctgctgccg
    11101 ctgcagggct gatcacacgg tcgaaccgga cgatgaatgg gaatggggcg cgagcttgtg
    11161 aacgagcttg tgaaagcgac tcgcgcccct atactgatct agtcggcgac aacgacgtta
    11221 cccgggggtg cactggcttc gacgggggca gcgaaatcgc acggcgcatg ccgagggggt
    11281 agctttcctc gttaatccag ctgcaaaaac ccagacgcca acgacgacgt ctacgctctc
    11341 gccgcttaag gcgtaagccc cgaacccact tgtgcccgtg ctcgtggatg tagggtcatt
    11401 atcacggaac cggccctggc ggcgacccgc caaccagtgg cttaactcta gcgggtgtgt
    11461 cctgcggcgt gcttcgcaca ccgtgttgtc gcagggcgag accaaacggt gagctaagca
    11521 tgtagtgccg ggcatggagt tccttcggac ggcggttcga ttccgcccac ctccaccatc
    11581 tacaccacat cgggccgcgc aagcggcccg atgtgttttc tgcgtcgacg agccttgcgg
    11641 acgacccgtt atgctggggc gatggcaacg ctgcatcctt ccccagattc cgatgcgctg
    11701 ccggcgctgc gccgcatgcg ctacggatac ccgttcgcga tcctcgtgct ggtcgtgtcg
    11761 gtgctgttcg tgctggtggc ctgggaccat gcgcgccagc gcgagttgcg gctggcgtcg
    11821 cagcagttcc gcggcatcgc ggggcagcag gcgtcgctgg tgcagatgca cctcaacaac
    11881 atcgaggtca ccttgcgtgg cggcgcatcg ctgttcgcgg cggtcgacca gcccacgccc
    11941 gagcaatggc gcggttacgt ggacggtttg cgggtaccgt cgcgcttccc gtacgtgctg
    12001 ggcttgggtt acgccgtgta cgtggatggc cacggattga cccgcctgca agaggcttgg
    12061 cgcgagcaag gccgtggcct cctggagatc cgcccacgcg gccatcgcga gcgctacggg
    12121 ccgatcctct tcctggagcc gcactccccg gggaaccgcg ccgcacttgg cttcgacatg
    12181 tatgcggagg caacccggcg cgccgcgatg cgcgcggcca tggagagcgg gcgatcgcaa
    12241 ttgagcggtc gcgtggtgct ggtacaggac gcggcgcagc cggagtattc gctggtgctg
    12301 tacatgcccg tgtatgccgg gttggaggcg acggagtcgc cgcagctgcg gcgggactcg
    12361 atgcgcggct gggtgtacgc gccattccgc atcggcgcga tgctgcgggc tgcgctgcca
    12421 ccggcgcaga cgcggacgcg gttgcgcgtg gtggatacca ccgatggcgg gcacgtggtg
    12481 atgcaccagg atgccggcat cgagacaacc cgacacgcct tcacccacag cctgacgatg
    12541 cagttctacg ggcgtcgatg gcgcttcgat ttcttctcgg gcccgaccgc aagcgccgcg
    12601 ccgcaactgg cggccctcga caagctgctc ttcgccggcc ttgccgcgtc cctgttgctg
    12661 ttcgcgatga cctggatgct ggcctcgacc gaggcgcggg caaggcgcat cgccgccgac
    12721 atgacccaga gttaccgccg cagcgaacag cgtttccgca atgccatgca gtattcggcg
    12781 atcggcaagg ccttgctgga ttcgaatggt gcgatcgtcg agagcaaccc tgcgttcggc
    12841 cggatcttcg gacgcagcga cagcaccttg cgggggcact cgctcaacga attcctggac
    12901 gaaggcgcta cccagccact ggtgacctcg cagatgcagg ccttcgacga gtcgggcggg
    12961 gtaatccgcc tgacccgcac cctgcgccgc ggtgacggcg aggtgcgcca cgtgcacctg
    13021 accttcgcgc cggtgccgaa cgagccgggc atcgacgtcg cccgcctggt gcaggtcgag
    13081 gacgtcagcg aacgcgtgcg cgcggaggcc gcggtccagg cgctcaaccg gacactggag
    13141 gcgcgcgtcg ctgcgcgcac ccgcgagctc agcgaggcca accgcgagct ggaatcgttc
    13201 gcctacagcg tgtcccacga cctgcgcgcg ccgttgcgcg ggatcgaggg attcagccgg
    13261 ctgctcggcg agcgccatgc gcatgggttg gatgccaccg ggcgcgacta cctggagcgc
    13321 gtgcgccgcg gcactgcgcg gatgggggag ttgatcgacg cgctgctgaa gctggcgcgg
    13381 atcggccgtg ccgaacccga ggtggcggag gtcgacctca gtgcgttggc cgcggaggtc
    13441 gcggcgggcc tggccgatgc cgatccgggg cgcaacatcg acctgcgcat cgaacccggc
    13501 atgcgcacgc gcggcgaccg cacgttgctg cagaacctgc tcgaaaacct gctgggcaat
    13561 gcctggaaat tcacccgcgg gcgcgacgac gcgcgcgtcg agttctgccg ggagcccggc
    13621 ggcgacgacg aggcatggtt cgtgatccgc gacaacggcg ctggcttcga ccccgactac
    13681 gccaacaagc tgttcaagcc cttccagcgc ttgcacagcc aggacgagtt cccgggtcac
    13741 ggcatcgggt tggcctcggt gaagcgcatc gtcgagcgcc acggcggcga gatccaggcg
    13801 gaaggcagcc ccgggcaggg cgcgacgttc cggttcactc ttgggacttc gacggaagtc
    13861 cgtatggacg atggaacccg ctagcccccg agtcgggggc gattcgcgca gaaggcgcga
    13921 atgggggttg gaagcataca gtggggccca tgattcgcca gcgaatcatg ggggcgtttc
    13981 cggcgatgcc gaaaccgtca cgcgtccttg ttgtgctggc gcatcacccg ttgcttctcg
    14041 cggttccagt cgcgctcctt gctggcctcg cgcttgtcat ggctctgctt gccgcgcgcc
    14101 agagcgagtt ccatcttcac cttgttgccc ttccagtaca gggcggtggg gatcagggtg
    14161 taaccgtcgc gctgtacttt cccgatgagt tcatcgatct cgtggcgatg cagcagcaac
    14221 ttgcgcgagc gccggtcgtt ggcgaccacg tgggtggacg cctggatcag cggcgtgatc
    14281 tgcgcgccga ccaggaacag ttcgccctgc agcaccaccg catagccatc ggtcaggttc
    14341 gcgcggcctg cgcggatcga cttcaattcc cagccctgta gcgacagccc ggcctcgaac
    14401 gtccgttcga agtggtattc gtggcgcgcg cgcttgttga gcgcgatggt gccgccgccg
    14461 tttggcttat ccttgcccgt cttcttgttc atcccgccat tctcgccgat ccgcccgcgt
    14521 cgcggcgtgt cggccatgct ggagtcgcga tgccggtcat cctgcgcagt gccctggtcg
    14581 aacattccgc cgcgcgcatg ttcgcgctgg tcaacgacgt ggcggcctat tcgcggcgat
    14641 tcgactggtg tcgcgaggcg caggtgctgg agcagggcga tgatcgcctg gtggcgcgcc
    14701 tggacctggg gttgggggga ttccatacct ggttcaccac cgagaacacc ttgtccccgc
    14761 cgcaccacat cgacatggcc ttgcgcgacg gcccgttccg ccggctcgat ggccgctggc
    14821 agttccacgc cctcgacgag tccgcctgca aggtcagcct gcgcctggaa ttcgagccgc
    14881 agaaccgatt gctggggccg gcgttgtcgc tgggcatgca gggcctggcc gaccgcatgg
    14941 tcgacgactt cgtgcgcgag gccgacaagg gcggggcctg atgcacatcg acgtgctgcg
    15001 cgcgtggccg catcgccatg aatcacgggc agtcgaggtt ggcgaaggcg cgactgtggc
    15061 caccgtactg gcggcaagcg gctgggggat ggacgcagag ttcaccgcgg ttgcggtgtt
    15121 cggcgttgct gcaagcagcg acaccgtgct gcacgaaggc gatcgcgtgg agctgctgcg
    15181 cccgctacag ctggacccaa agcaggcgcg gcgcctgcgg gccgaactca ggccgctcaa
    15241 gtcggcccgt tgaacacctg gctttagcgg ccgcccttct tcttgtcttt ctgcaggttc
    15301 gggccaaacg acttggtcgc tgtcttggcc agttccgaat cttcttcggg gaagtattcg
    15361 ccttcccatt tcgacaggct gccgttctcg aaccagagcg tcatgttctt gacctcggtc
    15421 gggccactgc gtccggtccg acggctggcc acgtaatccc agcgatcatg gtggaacgga
    15481 tcctggaccg acggcgtgcc cagcagtgtc atcacctggc gcttgtccat gccctgctgc
    15541 aactggtcca cggcggtctt gtcgagcagg ttgccctggt agatcggctg ccggtacagc
    15601 acgccgcaac cgctggcgaa caggaccacg aggacgatag caagcagctt gcgcatggag
    15661 gattggggcg agtggcggaa acgccgatga tacactccaa gcccgctgcg acggcgttcg
    15721 gggcctcacc gcccgacggc tgcagggcga tgcattcacc cgccgttggc gatcgaagaa
    15781 cccatgccgg agggccgatg gaatcgcagg acttacgcaa ggcaggcctc aaggtcaccc
    15841 atccgcgcat gcggatccta gaactgctgg aacagaccaa gccgcggcac atgaccgccg
    15901 aggacatcta ccggcaactg ctgggggatg gcgaggaaat cggcctggcc acgatctatc
    15961 gcgtgctgac gcagttcgaa tcggcgggcc tggtgctcaa gcacaatttc gaaggcggcc
    16021 atgccgtcta cgaactcgat cgtggcgagc accatgacca catggtcgac gtggacaccg
    16081 gcaaggtcgt ggagttccac agcgacgaga tcgaggagct gcagcgccag atcgccgcgg
    16141 cgcacggtta cgagatcgag gatcacgcgc tggtgttgta cgtccgcaag aagcgttgac
    16201 cgggcgtctt ccgcatctgc gctgtccctg gtgcggcgtg actgtcaggg ccggggtcgc
    16261 tagaacccgt ccagcagctt cctcgccgcg gcgcgcgctt cgtcgctgac ttgggtgccg
    16321 cccagcatcc gcgcgatttc gtcgatgcga ccttcggcat cgagcacgac gaccgacgat
    16381 tgggtcatgc cctccgccgc ggccttgctg acccggtagt gcgaatgccc ggccgccgca
    16441 acctgtggct ggtgggtgac gcacagcacc tgtcgcggcg caccgagcgc acgcagcttg
    16501 gcaccgaccg ccgcagcgac ggcgccaccg atgcccgaat ccacttcgtc gaacaccatc
    16561 gtcgccaccg cgtcggtgcc cagtgtggcg acttcgatcg ccagcgaaat ccgcgacagc
    16621 tcgccgccgg aagcgacctt gcgcaagggg cgcggcggct ggccggcgtt ggccgacacc
    16681 aggaagtccg cgcgctcggc accctgcgga tccggcgtgt cgctgtcatc gagttcgagc
    16741 agcacctcga actggccacc ctgcatgcca agctcggcga tcagtgtcgc cacggactgg
    16801 gcaagcgcat cgcctgcctt gcgccgcgag gctgtgagct ctgcggcggc ctgggtccat
    16861 gcggcatgcg cctgggtgat gtcgcgctcg atccgctgca ggcgttcgtc ggcatcggcg
    16921 agcgtgtcga gttcgcgcgc cagcgactcc cgatgcgccg ccagtccgtc gggcgcgaca
    16981 cggtgcttgc gtgccaggtc ctgcacgcgg gccaggcgcc gctccagcgc atccagcgca
    17041 tccggatcca ggtcgaggtc gtcgcggatg cgttccagca tcgcgaaggc ttcgtccagc
    17101 tggatcgcgg ccgaatccag caaggcatcg acgtcgccaa gccggggttc gtgcgtcatc
    17161 tcgcgctgca ggctgccgcg caggtgatgc aattgcgcgg cgacggcatg gtcgccaccc
    17221 agttgctcca gcgcgtggtc gcaggccgcg atcagcgacg ccgcgtgggc ctgccggcgg
    17281 tggctggcat ccagttccgc cagggcttcg ggcgccaggg tttcggcgtc gagctcctgc
    17341 aactggtggc gcaaccagga ctgccgctcg ccgacgtccc cacgggcgag cagggcgctg
    17401 cgttcttcct gcaacccctt ccattgcagg gcggtgctgc gcacggcctg cagcaagccc
    17461 ggatggcgcg catgcgcatc gagcaggcgg gtctggtgcg cgcgcgacag cagggcctgc
    17521 tgctcgtgct ggccgtggat ctcgaccagc aatcccgcca gttccgccaa ctgcgccaac
    17581 ggcacaggtc ggccattgat ccacgcgcgc gatccaccat ccgcacgcag ggtgcgacgc
    17641 agctggcagg cgtcttcgtc gtcgagttcg gcgtcgcgca gccaggccat ggccgcgggg
    17701 cagtcgtcca gggcgaattc ggctgccagt tcggcgcgtt gcgcgccatg gcgcaccgcg
    17761 ccggcgtcag ctcgtgcgcc accgaggaag cccagcgcat cgaccagcag ggacttgccg
    17821 gcgccggttt cgcccgagac gacggtcagg ccgcgcccga aggccagctc cgcatgggcg
    17881 accacggcga aatcgcgaat ggacagtcgg ctcagcatcg gcgcgcattg tgcctgcccg
    17941 cgggggcgtt ccggtagcgc ttgcatgccg gcggcgtggc gcatacaacc gtcgcgtgag
    18001 ccgccgtccc tccgacatcg ccagcgatcc ccgtgcgcgc cagttgctgc gcacgctggt
    18061 ggcccgtcat gtccgcgacg gcgagccggt cggttcgaag accctggccc ggcacgccgg
    18121 gctggatgtc agcccggcca cgatccgcaa catcctggcc acgatggagg aggccggcct
    18181 gctggtcgca ccgcacagtt ccgccgggcg catccccacc gcccaggggt accggctgtt
    18241 cgtcgattcg ctgctgcagg tcaagccgct gggcgatgtc gaactgcagc gcctgcgcag
    18301 cgagctgccg gtcggggccg gtacccaggc cttgctgggc aatacctcgg agttgctgtc
    18361 ggcgatgacc cgcttcgccg gcatggtcag cgtgccgaag cgggagggat tcgcgttccg
    18421 gcagatcgag ttcgtggcgc tggatccgca gcgggtgttg gcgatcgtgg tgtttgccga
    18481 tggcgaggtg cagaaccggg tggtgcaggt gcgccgcgcc ttcgatcccg ccgagctgga
    18541 gcagaccgcg aactacctca atgcgcattt tgccgggcag ccgctggcgc agatccgcgc
    18601 cgcgttgctg cgcgacctgc gggatgcgcg cagcgagatg gagcgcttgc tggcgtcgtc
    18661 gctcgacctc gccgaacagg ccttcgcgcc gggacgcgag gacgcgccgt tccacggcat
    18721 gctggtggcc gggcagaccc ggctgatggg cctgcaggac ctcaacgacc tcgatcgctt
    18781 gcgcgccctg ttcgaggcct tcgccgagaa gcgcgaactg ctgcaattgc tcgagcgcac
    18841 cgccaaggcg cccgggatgc gcgtgttcat cggcgaggaa accggcatcg ccccgctgga
    18901 gggcatgtcg ctggtgacgg cgccttacgg ccgagatgga cgcgtccttg gcgtgctcgg
    18961 cgtgatcggg cccagccgga tggcctacga gcgggtgatc ccggtggtcg aagccaccgc
    19021 ggccatcctc ggggccgcgc ttgacgcacc gtcctgaccc gttcgggcaa ggtacagcgg
    19081 gatcgcgcct tgaatccgtt ttgaccaagc cccatcacca tgccgggcgc ggcggatggc
    19141 cgcatcaagc acgggaacca acatgcagga tccaatccag gacgtgaacg acgcggccgg
    19201 cgccatgccg caggacgatg aactcgaggc gctgcgcggc gaactcgagc aactgaaggc
    19261 gcagtcgctg ctggaacgcg ccgacctcga aaaccagcgc aagcggatcg cccgcgagat
    19321 cgacagtgcc cgcaagttcg ccaacgagcg cctgctcggc gacctgttgc cggtcttcga
    19381 cagcctggac gccggcttga ccgcggccgg cagcgagtcc agcccgctcc gggacggcct
    19441 ggagatgacc tacaggcaat tgctgaaggt cgccggcgag aacggcctga gcgtgctgga
    19501 cccgaccggc gaggcgttca atcccgagca ccaccaggcg atcagccaga tgcaggccgc
    19561 cggcgtggcg gcggaccacg tggcccaggt gttccagaag ggctacctgc tcaacggtcg
    19621 cctgctgcgc ccggcgctgg tcgtggttgc ggcctgaggc aaaggctgga tcgcatcacg
    19681 cgggcctctt gaagccgccc ggcacaaccc cacatcacgt tcatcggcgc acggcgcccc
    19741 ccgacacacg cattgcagag gaaccaacat gggcaagatc atcggcatcg acctgggtac
    19801 caccaactcc tgcgtcgcca tcatggaagg cggcaaggcc aaggtcatcg agaacagcga
    19861 gggcgatcgc accaccccgt cggtggtcgc atggtcgaag gacaatgaag tgctggtggg
    19921 cgcgtcggcc aagcgccagg ccgtgaccaa cccgaagaac accttctacg cggtgaagcg
    19981 cctgatcggc cgcaagttca ccgatgccga ggtgcagaag gacctgggcc tggtgcccta
    20041 cgcgatcacg gcgcacgaca atggcgacgc ttgggtgtcg ctggtcgacg gcaagaagct
    20101 ggcgccgcag gaagtctccg cgaaggtgct ggagaagatg aagaagaccg ccgaggccta
    20161 cctgggcgag ccggtcaccg aagcggtgat caccgtgccg gcctacttca acgacagcca
    20221 gcgccaggcg accaaggacg ccggccgcat cgccggcctg gacgtcaagc gcatcatcaa
    20281 cgagccgacc gcggcggcgc tggcctacgg catggacaag ggcgagaaga ccgaccgcaa
    20341 gatcgcggtg tacgacctcg gcggcggcac cttcgacgtg tcgatcatcg agatcgccaa
    20401 tgtcgacggc gagaagcagt tcgaggtgct ggcgaccaat ggcgatacct tcctcggcgg
    20461 cgaggacttc gacaagcgcg tcatcgacta tctcgtcgag gaattccaga aggagcaggg
    20521 catcgacctg cgcaaggatc cgctgg
//
LOCUS       DAOOCX010000038        19750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2422_length_19750_cov_8.414572, whole genome shotgun sequence.
ACCESSION   DAOOCX010000038 DAOOCX010000000
VERSION     DAOOCX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 19750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19750
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2422_length_19750_cov_8.414572"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PK399_05415"
     CDS             <1..643
                     /locus_tag="PK399_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW12590.1"
                     /translation="NHPEDGAIAPSVFIEVAEQSGLIEAIGPEVLRSACAEAAGWPTP
                     PGAGEPPFVSVNVSPRQLRSGDLVEQVTGALVESGMAPERLHVELTETAVISDEANAT
                     ALLARLREVGVRVWLDDFGTGYSSLSHLRRVQVDGLKIDRSFITDLLRDPDDLALTSA
                     IIAMAHSMGITVVAEGVERQGQFDLLRERDCDLAQGYWLGHPMSALEFARLLD"
     gene            complement(674..1315)
                     /locus_tag="PK399_05420"
     CDS             complement(674..1315)
                     /locus_tag="PK399_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HPW12591.1"
                     /translation="MPSTPEPAAGTDRHDAGTRAILDALLDGDPTQSLDFDDLLDGLG
                     RRAFGMLLFVAALPAFIPIPIGGALSGPLIMLVAAQLLVGMAHPWLPGFIARRGPKRQ
                     ALARFERMVDPWLARLERIVRPRMTAVLDHPLATIFTGLLLLLLGLLLALPIPLTNYV
                     FGGMILVYALALLERDGALMLAAWGLGIVALVTTGALSGGLITALQGWIDTTF"
     gene            complement(1299..2609)
                     /locus_tag="PK399_05425"
     CDS             complement(1299..2609)
                     /locus_tag="PK399_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPW12592.1"
                     /translation="MVELLVVLFLILLNAFFALSEMSVVTSRKARLKQRAEDSAGARK
                     ALELAEHPERFLSTVQVGITTIGILTGMFGGDAIGAAIGVWIAKHLPAVGKYAGTIGT
                     VLAVGLITYLSIVLGELLPKRLALLAPERLAGIVALPMHWLSRAASPAVWLLSATVSA
                     LLRLLRVNRTDSSQVTEEEIHMLVSEGHEQGVIDLDERNMLNRVMRLGDRTADSLMTP
                     RTRIAWLDAAAPLAENLATMQEHEFSRYPVYRGDDSDVAGVLEVKTLLDELGKDQPAL
                     FQDLRAPVFVSESTHAMKLLEILREEQLSMALVVDEYGDIQGIVTITDIMDAVLGRLQ
                     AGEADGDEALVVERQDGSLLVDGGLHIDELRELTGERLADADEHGYNTTAGLTIAYFG
                     RIPHVGEYFQLGQWRIEVVDLDGPRIDKLLLQRMPDALADAVDA"
     gene            2816..3442
                     /locus_tag="PK399_05430"
     CDS             2816..3442
                     /locus_tag="PK399_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPW12593.1"
                     /translation="MFSLQTIFGSGKQFYLLLDEAASAAYDSAKALHVMMKATDRQPA
                     LDAFKLARQRERTASDKIGQALVDSFITPIEREDIEALGSALYKIPKQIEKFADRYAL
                     ATMHLEQIDFAPRAAMLEQAAGVVVEMVHELERLNIDSMTALNDKLRSIENEADRLML
                     ELYREIYSGRLDSLQMFLLKEFFEILEKAIDRCREAGVVAYQIVLKNS"
     gene            3455..4576
                     /locus_tag="PK399_05435"
     CDS             3455..4576
                     /locus_tag="PK399_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPW12594.1"
                     /translation="MLTLVLVVIVVALVFEFINGFHDTANSIATVVATKVLTPGQAIM
                     LAASTNLIGAFMGTAVAKTIASGLVDTEVVTVTSQVLICTLLGAIIWNLITWWRGLPS
                     SSSHALIGGLCGAAMAASQNNWASLIWSKAGDGDWASNKGLLWKVVVPMVTSPLAGFL
                     LGVGIITLLYLIIASLMKAGRPLSKLAKPRWVNAFFGKAQLVSAAYMGFAHGRQDAQK
                     TMGIIALSIFGAQAAGTLDNLPAWLSFLHPDGGKDDIDSWVIATCALVMAAGTAAGGW
                     RIIKTLGHKMVKLQTIDGFAAETASATILVTAAHFGMPVSTTHSISTAIMGVGFAKNP
                     RKLKIGVIERIVWAWILTIPAAGGIAYGLWRLIDALGWA"
     gene            complement(4652..5062)
                     /locus_tag="PK399_05440"
     CDS             complement(4652..5062)
                     /locus_tag="PK399_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12595.1"
                     /translation="MDNFEQIRAHLVSAGYKLTLQDPFLACVELSLAQGRRHQAIFLS
                     ELQDDDGRNYLRVSTAVAPITGIDARRALAFNWENRVGYLAIGELDGVPYLQLCENRP
                     YDCVDAAEIDRLVLEIGGTGDEMERKMSANGDLL"
     gene            5102..5464
                     /locus_tag="PK399_05445"
     CDS             5102..5464
                     /locus_tag="PK399_05445"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPW12596.1"
                     /translation="MPDKPARSPAAVDVPAADRILAAIRAVPRGSVAGYGHIAQRAGL
                     PGRARLVARILGQNEDPGLPWHRILRSDGRIAFPAGSPGFEEQARRLRTEGIEVRNGR
                     VRMPESDESLDAAMWRPR"
     gene            5522..6202
                     /locus_tag="PK399_05450"
     CDS             5522..6202
                     /locus_tag="PK399_05450"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPW12597.1"
                     /translation="MPFNLPRITKALLIANIAGFALQWLLGDARMQVFELWPLGDSLY
                     DPSGSAPSFMPWQLLTYGFLHDPSNIFHLAFNMLALVMFGAPLEYTWGERRFLTYYLV
                     CVFGAGLCQLLVGSIAVSQGELAYRTVGASGGVFGLLLAYGMLFPNQRVMLLFPPIPM
                     KARTLVILYGVFELSLGLTGLQPGVAHFAHLGGMLFGWLLIRYWRGQPPFGKRKPPDR
                     RPPLRVVR"
     gene            complement(6239..8251)
                     /locus_tag="PK399_05455"
     CDS             complement(6239..8251)
                     /locus_tag="PK399_05455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HPW12598.1"
                     /translation="MPTPRPLAFALLVALVAGSAAPDSLAAKKPAAKKKAPAVAVACS
                     DFHAEANKAWLASNPIPPSGAISALGQLTARAQQQQRELLDASMQSPQGNVQKLLGDF
                     WASGLDEAAVERDGANPVAPLLKRIDGIRKTGDVAPAIAALHQVGIPALFNFTADVDL
                     RALDRHLGYFSQGGLGLPDPAYYSRTDADTQALVARYADYIRKILVLTGIPAKDLERE
                     TALVLDLEKRIAASARPLVELRDPKTNFAPVATAGLGKQYRNLQLDAFLRAQGVSDDA
                     VSIANPAMFAQLDALVGSLKPAQWKTYLRWRVGDAMAPYLAKPWRDASFAFRGKVLLG
                     QAEPAPRWQQTLDAINLAAGPMLGREYAARYLPDATRQQALAIATQVRDALQRNVDNS
                     TWLGEAAKQEARKKIGKLGIEIGAPKRDLDYSVQPMGRGSFGSNMLIASTWRHREEMK
                     RIGRGNAERRWDVLPQQPALAYDLAHNRLIVTAAMLQAPVFDPAMPLGGQYGAFGALV
                     GHELSHAVDGRGRMVDADGNVRDWWTGAEAAAWDTTAQRVVSLYDTLVYPGLPALKVN
                     GKLTRDENVADLAGLELARAALVAAQPTGGVAADKAFYSAWAQVWAQQVTADEAQRRS
                     LQDVRAPGQWRTNAPIMQQGAFGSAFACKVGTPMQPKPEARISVFH"
     gene            complement(8337..10115)
                     /locus_tag="PK399_05460"
     CDS             complement(8337..10115)
                     /locus_tag="PK399_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HPW12599.1"
                     /translation="MNPSFSDAPRHADFLGHPKGVYVCFFTEMWERFSFYGMKALLLL
                     YLTKYHLFSDTGGLNLLGAYGGLVYCLPVLGGLLADRWLGMRKAVVFGGVLLVLGHLG
                     MAVEGEQARKVGDTIVRDDAALLVFYGSLALIIMGVGFLKPNISVIVGKLYAEDDPRR
                     DSGFSLFYAGINLGAMLASLVCGVLGETYGWKYGFGAAGIGMLAGLAMFLWGQKYLHG
                     MAEPPSPERLRERVAGMPREWAIYLATLLGLLPIAGLMWATGNDAFTLSIGVDLTLAQ
                     LLMLAVLAVVLAWFTRFVLRDCTPRERGQMLTLMALIFIALLFYTLYEQTYGSWVVFT
                     DRLLTKDFFPSLVLAEGLPFPYFVICTLLLPAAFMLAMRWQSRSPGSRLPTLVFLAAA
                     AVTLLMVVGMVVLQPQTAGSLTFISGIFILSFAPLFSWLWSWLDRRGRNPGKPLKSVL
                     GLLFGALSFLPLYLAAHGAGDAGTFASVWWLVLAYAVLQVGEICLYPVGLSAVTQLSV
                     PRVASLMMGTWFLATAFSELLAAKLGTLASIEDPGAIAEHSSAWTSAATTYGDYFWSL
                     ALGGLACTVLAALALPWLNRGMHGVK"
     gene            10322..12262
                     /locus_tag="PK399_05465"
     CDS             10322..12262
                     /locus_tag="PK399_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HPW12600.1"
                     /translation="MANSGSGTPQLTLRAVILAVVLAMILSAANAYLGLFAGLTIATA
                     IPAAVVSMGVLRLLGGGTILENNIVQTGASAGSSIAAGVIFTIPALIILGYWQDFKYS
                     WVLAIAGLGGLLGVLFSVPLRRSMIVEDPLPFPEGKAAAEVLKAGENPGPGLKILGIS
                     ASIGALVKLAAESGFRMIPDNAAGAGFFGKYLGYMGTGLSPALLGVGYIVGLNIGIVV
                     VSGSVFAYNLAIPFYHAFLLNGDPEVAAKVVGKTAAEAAAIIRGDRIRYLGVGTMLVG
                     GIWTLFSLRNSLLSGVKSGIAAARKGADANLPETERDLPMKWMLIALLLFVLPLLGLY
                     QAIVQNWGVSIPMTIIMIVAGFLFVSVSAYLAGLVGSSNNPVSGITIATILFASVVLM
                     LLLGSDSKIGAVAAIMIGAVVCCAAAVGGDNLQDLKAGYIVGATPWKQQLMLGIGAFS
                     CALIMAPVLNLLAQAYGIGDPTRPGSLQAPQATLMASVAQGLFGGKLPWNMIIAGGVI
                     GAIVIAIDGWLKSRNSTFRVPVLAAAIGIYLPLELMVPIFLGGLLAHFVEKRHGIVGT
                     HDEALRDRVHRPGVLFAAGLITGEALMGIAIAIPIVMTERADVLALPEQFHFGQFVGL
                     VVLAIVGWLIYRTGAKANPNAA"
     gene            12335..14413
                     /locus_tag="PK399_05470"
     CDS             12335..14413
                     /locus_tag="PK399_05470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017169463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPW12601.1"
                     /translation="MKIRNALLPLALALSLPAVADATRGFEVRDMAYLDRHSSPTLSP
                     DGRVLVFAKRVVDKGTNKSSTSLWTRNLLTRDLRPPSRLTPEGWNVNSPSFSADGKTV
                     YFMSGKSGSMQLYSMPLAGGEPTRLTDYALDVDAYKLSPDGTQVALAFAVFPDCKADL
                     DCTKKKLDDVDALKTSGKVFDRMFIRHWDTWNDGRLNRVFAAKLGGAGMLKSATLLSG
                     DIAGDIPSKPFGDLSDFAWSADGKRVAMSVRQANREEPWSTNFDLWLVNADGSGARNL
                     TAKNPAWDAGPVFSADGKTLYYRAMARPGFEADRFALMAMDLASGQSKEIASKWDASA
                     DGITLSADGKWIYTTAGELGRHPLFRVSLANGEVESVVKDGSVSSFDLAGQTLAISRN
                     SMKSGDVIYTTSADGKAPLRAITPSAGEMLPDVAWGEYEQFNFIGWNNETVHGYVVKP
                     WNYQPGKKYPVAFLIHGGPQGSFGDGWSYRWNPQTYAGQGYAVVMIDFHGSTGYGQKF
                     TDAISGHWGDRPLEDLQKGWAAAQEKYAFLDGEKACALGASYGGFMVNWIQGNWYDKA
                     GNSPWKCLVSHDGVFENNAMGYATEELWFSEWENGGTPFDNPKGYQQFNPANHVANFK
                     VPMLVIHGQLDYRIPVEQGIALFSALQRKGVESKFLYFPDENHWVLKPQNSVQWHDTV
                     NGWLKQYIGQ"
     gene            14416..15669
                     /locus_tag="PK399_05475"
     CDS             14416..15669
                     /locus_tag="PK399_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPW12602.1"
                     /translation="MEPVAGTALITNEAAVLGLLAVILGFVFWGASRERGFWKKFYAV
                     VPALLPCYLLPAIANSTGLIDGAGSGLYPMARDYLLPASLVLFCVSMDIGGIARLGPK
                     ALVMFLTGTVGVMLGALVSFTVMGALHPETVAGETWRGMTTLTGSWIGGGANQAAMKE
                     VFAVDATLFGQFAAVDVIVGYVWMAALLFMASRAEAWDRWLKADTSAIETLKQRMATY
                     QAQHARIASLTDLVLIFAVAIGATGLAHFLAEPLVAWIKSMPLEWRLQDYSLDSRFFW
                     IVVIATAIGVVASLTRARELEGAGASKIGTVFLYVLIATIGMQMDLKALADKPFLLLL
                     GLIWISVHAGLMLLVARLIRAPLFFLAVGSQANIGAAASAPVVASAFHPALAPVGVLL
                     AVLGYVLGTYCAYLTGLMLQALAGG"
     gene            complement(15671..16672)
                     /gene="corA"
                     /locus_tag="PK399_05480"
     CDS             complement(15671..16672)
                     /gene="corA"
                     /locus_tag="PK399_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPW12603.1"
                     /translation="MNHQHISDQPACVVNCAAYDRAGRRRDIHLDAISDVLAVDDGSF
                     VWVGLYEPEDALLDKLQEEFGLHDLAIEDAQNAHQRPKLEGYGSTLFIAAHTAQRVEG
                     HVRFGETHLFVGPRFLVTVRHGASLTFASVRQRLEREPEMLAQGPTAALHAVLDFIVD
                     NYQPIVSDFEQELDALEHEVFAESYRRGTIRRLYELRKELTQMRMAVAPMQDILSQLM
                     RNPSLAIPDEVKPYFRDVLDHAARVGDTIDTLREMVTAAMNVNLSLVTVAQGEIVKRL
                     AGWAGLLAAPTLIASWYGMNFEHMPELHGRYSYWILIGGVALFCTGLYRYLRKVHWL"
     gene            complement(16714..17946)
                     /locus_tag="PK399_05485"
     CDS             complement(16714..17946)
                     /locus_tag="PK399_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HPW12604.1"
                     /translation="MRITDALRVATALLLACALGLAHAQSVEPSGSASPVLAPASDAP
                     RIGVVTMGPGDVFWERFGHDAIVVDDGAPAGPTSYNFGFFDLAEDGFIGRFVRGEMEY
                     MLVALPLEDDLRYYREVGRGARLQWLDLDPAQARSLAAALAENAKPENARYRYDYYTD
                     NCASRVRDAIDRALGGQLRRQLDVRSSGDTYRTESVRLASPAAWMRVGFDLGLGPFAD
                     RPLTRWQQAFLPRRLADDLREATRADGRPLVAEEIELLPQRQAAEPVGRAPRLWPWLL
                     AGVLAGTAVLVLAGWRPRLLAGFAGAFWATCGLLGLVLALGWAFTAHHALWANRNLLL
                     LNPLCLALIPGAWALLRGRMPSSRFRTVLIVLAAMAALACLPLWLQAQPQRNGHWIAL
                     LLPIHAALARAWARRPHA"
     gene            complement(17933..18811)
                     /locus_tag="PK399_05490"
     CDS             complement(17933..18811)
                     /locus_tag="PK399_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HPW12605.1"
                     /translation="MSEDDSRQDADSAERSGDKPRRSWLDRISAALSGEPTTREDLVE
                     LLRDVQADGLIAADTLRMMEGAIAVADLTVGDVMVSRAQMVALPADGKFLDLMKQVVE
                     SGHSRFPVHGEDKDEILGILLAKDLLRGVVADNGPGTIRELLRPAVLIPESKKLNVLL
                     REFRQSRNHMAIVIDEYGGVAGLVTIEDVLEQIVGDIDDEHDEAEDDSRLIAAQDDGQ
                     FMVDALTPIDDFNARFGADFDDDEYDTIGGLVTDAVGHLPEVGEELALGRFGFRVARA
                     DARRLLSLHVTVHADH"
     gene            18990..>19750
                     /locus_tag="PK399_05495"
     CDS             18990..>19750
                     /locus_tag="PK399_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12606.1"
                     /translation="MATDDGLLIKPEPRKPWVVVAVAAGVLAVLAGGYVASGLSGKPK
                     PAATATAASPAPVTANPDQLPAIIIAVLPLAADAGATAAPADGDASQHLVDALKGGSE
                     RGSAFNDGVSAQLAGALSQFDGVAVTSPESSFQQRDADAPPATIGKMLGATHLLRGTA
                     SRDGETLRIDAALVSVADGSTVWFDRYQRPYAELFKVQDEMAAAIGAALKVKRLPAPQ
                     GGQDERPPGGSLQAYEALLAGDAALERADADGVAAA"
BASE COUNT         3149 a         6505 c         6863 g         3233 t
ORIGIN      
        1 gaaccatccg gaggacgggg cgatcgcgcc ttcggtgttc atcgaggtcg ccgaacagag
       61 cggcctgatc gaggcgatcg gccccgaggt gctgcgcagc gcctgcgccg aggccgcggg
      121 ctggccgacc ccgcccggtg ccggcgagcc gccgttcgtg tcggtcaatg tctcgccgag
      181 gcagctgcgc agcggcgacc tggtggagca ggtcaccggc gccctggtcg aaagcggcat
      241 ggccccggaa cgcctgcacg tggaactgac cgagaccgcc gtgatcagcg acgaggccaa
      301 tgccaccgcc ctgctggcgc gcctgcgcga ggttggcgtg cgcgtgtggc tggacgattt
      361 cggcaccggc tattccagcc tcagccactt gcgccgcgtg caggtcgacg gcctgaagat
      421 cgaccgcagc ttcatcaccg acctgctgcg cgacccggat gacctggccc tgaccagcgc
      481 gatcatcgcg atggcgcatt cgatgggaat caccgtggtc gcggagggcg tcgagcgcca
      541 aggccagttc gacctgctgc gcgagcgcga ctgcgacctg gcgcagggct actggctggg
      601 gcatccgatg agcgcgctgg aattcgcccg cttgctggac tgacccgttg cggcaaggct
      661 gcgggcgcac ccctcagaac gtcgtgtcga tccagccctg caaggcggtg atcagcccgc
      721 ccgagagcgc gccggtggtc accagcgcca cgatgccgag gccccacgcc gccagcatca
      781 acgcgccgtc gcgttcgagc agggccagcg cgtacaccag gatcatcccg ccgaacacgt
      841 agttggtcag cgggatcggc agcgccagca gcaggccgag caagagcaac agcaggccgg
      901 tgaagatggt cgccagcggg tggtccagca cggcggtcat gcgcggtcgg acgatgcgtt
      961 ccagtcgcgc cagccaggga tcgaccatgc gctcgaagcg cgccagcgcc tgccgcttgg
     1021 ggccgcgccg ggcgatgaag ccgggcagcc acggatgggc catgccgacc agcagctgcg
     1081 cggcgaccag catgatcagc gggccgctga gcgcgccgcc gatcgggatc gggatgaagg
     1141 cgggcagtgc cgccacgaac agcagcatgc cgaaggcgcg ccggcccagg ccatccagca
     1201 ggtcgtcgaa gtccagcgat tgcgtcggat cgccgtcgag cagggcgtcg aggatcgcgc
     1261 gcgtgccggc gtcgtgccgg tcggtgcctg ccgcgggttc aggcgtcgac ggcatcggcc
     1321 aaggcgtcgg gcatgcgttg cagcaacagc ttgtcgatgc gcgggccgtc caggtcgacg
     1381 acctcgatcc gccactggcc cagctggaaa tattcgccga cgtgcgggat ccgcccgaag
     1441 taggcgatgg tcaggccggc ggtggtgttg tagccatgct cgtctgcatc ggccaggcgt
     1501 tcgccggtga gttcgcgcag ctcgtcgata tgcaggccgc catcgaccag cagcgaacca
     1561 tcctggcgct cgaccaccag cgcctcgtcg ccgtcggctt cgccggcctg caggcggccg
     1621 agcacggcgt ccatgatgtc ggtgatggtg acgatgccct ggatgtcgcc gtattcgtcg
     1681 acgaccagcg ccatcgacag ctgttcctcg cgcaggatct ccagcagttt catcgcatgg
     1741 gtcgattccg acacgaagac cggggcgcgc aggtcctgga acaacgccgg ctggtccttg
     1801 cccagttcgt ccagcagggt cttcacttcc agcacgccgg ccacgtcgct gtcgtcgccg
     1861 cggtagaccg ggtagcgcga gaattcgtgc tcctgcatgg tcgccaggtt ctcggcgagc
     1921 ggcgcggcgg cgtccagcca ggcgatgcgg gtgcgcggcg tcatcaggct gtcggcggtg
     1981 cggtcgccca ggcgcatcac ccggttgagc atgttgcgtt cgtccaggtc gatcacgccc
     2041 tgctcgtggc cttcgctcac cagcatgtgg atctcttcct cggtgacctg cgagctgtcg
     2101 gtgcggttca cccgcaacag gcgcaacagg gcgctcacgg tggcgctcag cagccacacc
     2161 gccgggctgg ccgcacgcga cagccagtgc atcggcaggg cgacgatccc ggccagccgc
     2221 tcgggcgcca gcagggccag ccgcttcggc agcagttcgc ccagcacgat gctgaggtag
     2281 gtgatcaggc cgaccgcgag cacggtgccg atggtgccgg cgtacttgcc gacggcgggg
     2341 aggtgcttgg cgatccagac gccgatcgcc gcgccgatgg cgtcgccgcc gaacatgccg
     2401 gtgaggatgc cgatggtggt gatgccgacc tgcacggtcg acaggaagcg ctccgggtgc
     2461 tcggccagtt ccagcgcctt gcgggcgccc gcgctgtcct cggcgcgctg cttcagccgc
     2521 gccttgcgcg aggtgaccac cgacatctcc gacagcgcga agaaggcatt cagcaggatc
     2581 aggaacagga cgaccagcaa ctcaaccatg gtcgtggccc ggcttgcggc tggcaccggc
     2641 gcatgccggc cacgtggccc ctgggcgggc acgggaggtc atcggcggcg ggcgtggggg
     2701 agtgtcgtcc atgggatgcg ccggggcgcg tcggcatcat aacagggcac cccgtgcgca
     2761 cgactgtcac cgagcggtca taatcccgcc cattcgcccc agcgcaggct ccaccatgtt
     2821 ctccctgcag acgattttcg gttccggcaa gcagttctac ctcctgctgg acgaagcggc
     2881 ctcggccgcc tacgacagcg ccaaggcgct gcacgtgatg atgaaggcca ccgaccgcca
     2941 gcccgcgctt gacgccttca agctggcccg ccagcgcgag cgcacggcct ccgacaagat
     3001 cggccaggcg ctggtcgaca gcttcatcac cccgatcgag cgcgaggaca tcgaggcgct
     3061 gggctcggcg ctgtacaaga tccccaagca gatcgagaag ttcgccgatc gctacgcgct
     3121 ggccaccatg cacctcgagc agatcgactt cgcgccgcgc gcggcgatgc tggagcaggc
     3181 ggcgggcgtg gtggtggaga tggtccacga actcgagcgc ctgaacatcg acagcatgac
     3241 cgcgctcaac gacaagttgc gctcgatcga gaacgaagcc gaccggctga tgctggagct
     3301 gtaccgggaa atctattcgg gccggctgga cagcctgcag atgttcctgc tcaaggaatt
     3361 cttcgagatc ctggagaaag ccatcgaccg ttgccgcgag gccggcgtgg tcgcgtacca
     3421 gatcgtgctc aagaacagct gacgggccgc cacgatgttg accctcgtgc tggtggtgat
     3481 cgtggtcgcc ttggtgttcg aattcatcaa cggcttccac gacaccgcca actccatcgc
     3541 caccgtggtc gccaccaagg tgctgacccc gggccaggcg atcatgctcg ccgccagcac
     3601 caacctgatc ggcgccttca tgggcaccgc cgtcgccaag accatcgcct ccggactggt
     3661 cgacaccgag gtcgtgacgg tgacctcgca ggtgctgatc tgcaccttgc tcggggcgat
     3721 catctggaac ctgatcacct ggtggcgcgg actgccgtcc tcgtcctcgc atgcgctgat
     3781 cggcgggctc tgcggtgccg cgatggcggc gtcgcagaac aactgggcgt cgctgatctg
     3841 gtcgaaggcc ggcgatggcg actgggccag caacaagggc ctgctgtgga aggtcgtggt
     3901 gccgatggtg acctcgccgc tggccggctt cctgctgggc gtgggcatca tcaccctgct
     3961 gtacctgatc atcgcctcgc tgatgaaggc gggacggccg ttgtcgaagt tggcgaagcc
     4021 gcgttgggtc aatgctttct tcggcaaggc gcagctggtc tcggcggcgt acatgggttt
     4081 cgcccacggc cgccaggacg cgcagaagac gatgggcatc atcgccctgt cgatcttcgg
     4141 tgcgcaggcg gccggcacgc tcgacaacct gccggcatgg ttgtcgttcc tgcacccgga
     4201 cggcggcaag gacgacatcg acagctgggt gatcgcgacc tgcgcgctgg tgatggcggc
     4261 cggtaccgcg gccggtggct ggcggatcat caagaccctg ggccacaaga tggtgaagct
     4321 gcagaccatc gatggcttcg ccgcggaaac cgcctcggcc acgatcctgg tgactgcggc
     4381 ccacttcggc atgccggtgt cgaccaccca cagcatttcc accgcgatca tgggcgtcgg
     4441 cttcgccaag aatccgcgca agctcaagat cggggtgatc gagcggatcg tgtgggcatg
     4501 gatcctcacc atcccggccg cgggcggcat cgcctacggg ctctggcggc tgatcgacgc
     4561 actgggctgg gcctgatacg gcacgggtta gtggaacgaa aaagccggcc agtggccggc
     4621 ttttttcatg cgggatgcac ggggatgcgg gtcagagcag gtcgccattc gccgacatct
     4681 tgcgttccat ctcgtcgccg gtgccgccga tctccagcac caggcggtcg atctcggccg
     4741 catccacgca gtcgtacggg cggttctcgc acaattgcag gtagggcacg ccatcgagtt
     4801 cgccgatggc caggtagccc acgcggtttt cccagttgaa cgccagtgcg cggcgggcgt
     4861 cgatgccggt gatcggcgcg accgcggtgc tgacgcgcag gtagttgcgg ccgtcgtcgt
     4921 cctgcagttc ggacaggaaa atggcctggt ggcggcggcc ctgcgcaagc gacagctcga
     4981 cacaggccag gaagggatcc tgcagggtca gcttgtagcc ggcggaaacg aggtgggcgc
     5041 ggatctgttc gaagttgtcc atcacgagtc ggtatcgggg gatgtggcta tgctaacccg
     5101 gatgccggac aagcctgctc gaagccccgc cgcagtggac gtgcccgcgg cggatcggat
     5161 cctggccgcg atccgcgccg tcccgcgcgg gtccgtcgcg ggttatggcc atatcgcgca
     5221 acgggccggc ctgccggggc gggcgcggct ggtggcccgg atcctgggcc agaacgagga
     5281 tcctggcttg ccctggcacc ggatcctgcg ctcggacggg cgcatcgcgt tccctgcagg
     5341 ttcgccgggt ttcgaggaac aggcgcggcg cttgcgcacg gaaggcattg aggtacgcaa
     5401 cggccgggtg cgcatgcccg aatcggacga atccctggat gcggcgatgt ggcgaccgcg
     5461 ttgagggccg ggatggtcgg ccgcgtatga tgcattgcaa cgaaaccgga ttgccgcacg
     5521 gatgccgttc aacctgccac gcatcaccaa ggccctgctg atcgccaaca tcgcgggctt
     5581 cgccctgcag tggctgcttg gcgatgcgcg gatgcaggtg ttcgagctgt ggccgctcgg
     5641 cgacagcctg tacgaccctt caggttccgc gccatcgttc atgccgtggc aattgctcac
     5701 ctacggcttc ctgcacgacc cgtcgaacat cttccacctg gcgttcaaca tgctggcgct
     5761 ggtgatgttc ggtgcgccgc tggaatacac ctggggcgaa cgccgcttcc tgacgtatta
     5821 cctggtctgc gtgttcggcg ccggcctgtg ccagttgctg gtcggttcga tcgcagtctc
     5881 gcagggtgag cttgcctacc gcacggttgg cgcgtcggga ggcgtgttcg gcctgctgct
     5941 ggcctacggg atgctgttcc ccaaccagcg ggtgatgctg ctgttcccgc cgatcccgat
     6001 gaaggcgcgc acgctggtca tcctgtacgg cgtgttcgaa ctctcgctgg ggctgaccgg
     6061 gctgcaaccg ggcgtggcgc acttcgccca tctcggcggc atgctgttcg gttggttgct
     6121 gatccgctac tggcgcgggc agccgccgtt cggcaaacgc aaaccgcctg accggcgacc
     6181 gccattgcgc gtggtgcgct gagattccgg atgcctgcct gggcaggcat cccgcgcatc
     6241 aatggaagac gctgatgcgc gcttccggct ttggctgcat cggcgtaccg accttgcagg
     6301 cgaaggcgct gccaaacgcg ccctgctgca tgatcggcgc attggtccgc cactggccgg
     6361 gcgcgcgtac gtcctgcagc gagcgacgct gcgcttcgtc cgcggtgacc tgctgcgccc
     6421 agacctgtgc ccaggcggag tagaacgcct tgtccgcggc gacgccaccc gtcggttggg
     6481 cggcaaccag tgccgcgcgc gccagttcca ggccggccag gtcggccacg ttctcgtcgc
     6541 gggtcagctt gccgttgacc ttgagcgccg gcagtcccgg atacaccaga gtgtcgtaca
     6601 ggctcaccac gcgctgtgcg gtggtatccc atgccgctgc ttcggcaccg gtccaccagt
     6661 cgcgcacgtt gccgtcggca tcgaccatgc ggccgcggcc atccacggcg tggctgagtt
     6721 cgtggccgac cagcgcgccg aatgcgccgt actggccgcc gaggggcatc gccgggtcga
     6781 acaccggtgc ctgcagcatc gccgcggtca cgatcaagcg gttgtgggcc aggtcgtacg
     6841 ccagcgcggg ctgctgcggc agcacgtccc agcgacgttc ggcgttgccg cgaccgatcc
     6901 gcttcatttc ctcgcgatgc cgccaggtcg aggcgatcag catgttgctg ccgaagctgc
     6961 cgcgccccat cggttgcacg ctgtagtcga ggtcgcgctt cggcgcgccg atctcgatgc
     7021 cgagcttgcc gatcttcttg cgcgcttcct gcttggcggc ttcgcccagc caggtgctgt
     7081 tgtcgacatt gcgctgcaat gcgtcgcgca cctgggtggc gatggccagc gcctgctggc
     7141 gggtggcatc aggcaggtaa cgcgcggcgt attcgcggcc cagcatcggg cctgcggcca
     7201 agttgatcgc atccagcgtc tgctgccagc gcggtgccgg ttcggcttgt ccgagcagga
     7261 ccttgccgcg gaaggcgaag gacgcatcgc gccacggctt ggcgaggtac ggcgccatcg
     7321 cgtcgcccac gcgccagcga aggtaggtct tccattgcgc gggcttgagg ctgccgacga
     7381 gcgcgtcgag ttgggcgaac atcgccgggt tggcgatcga caccgcatcg tcgctcacgc
     7441 cctgcgccct caggaaggca tcgagctgca ggttgcggta ctgcttgccg agtccggcgg
     7501 tggcgaccgg cgcgaaattg gtcttcgggt cgcgcagctc gaccagcggg cgcgcgctgg
     7561 cggcgatgcg cttctcgagg tccagcacca gggcggtttc gcgttccagg tccttggcgg
     7621 ggatgccggt gaggaccagg atcttgcgga tgtaatcggc gtagcgcgcg accagcgcct
     7681 gggtgtcggc atcggtgcgc gagtagtacg ccgggtcggg caggccgagg ccgccctggc
     7741 tgaagtagcc gagatggcga tcgagcgcgc gcaggtcgac atctgcggtg aagttgaaca
     7801 gcgcggggat gccgacctgg tgcagcgcgg cgatcgccgg ggccacgtcg ccggtcttgc
     7861 ggatgccgtc gatgcgcttc agcagcggcg cgaccgggtt ggcgccgtcg cgctccaccg
     7921 cggcttcgtc caggccgctg gcccagaaat cgccgaggag cttctgcacg ttgccctgcg
     7981 gcgactgcat cgaggcatcc agcaattcgc gctgctgctg ttgcgcgcgc gcggtgagct
     8041 ggccgagtgc ggagatcgcg ccggacggcg gtatcgggtt gctcgccagc caggccttgt
     8101 tggcctccgc atggaagtcg gagcacgcca ccgccaccgc aggcgccttc ttcttcgcgg
     8161 ccggtttctt cgcggcgagg ctgtcgggcg cggcactgcc ggcaacgagg gcgacgagca
     8221 gggcgaaggc aagcgggcgc ggggtcggca tgcggcatat ctcggatcga acgggtggcc
     8281 gagtgtagca gcggccccgt aagcggctgc tgacgatgga cgctatccgc agcgcactac
     8341 ttgacgccgt gcattccacg attgagccat ggcagggcga gcgcagccag cacggtgcag
     8401 gcgagtccac ccagcgccag gctccagaaa taatcgccgt aggtcgttgc cgcactggtc
     8461 catgccgagg agtgttcggc gatcgcgccg ggatcctcga tcgatgccag cgtgcccagt
     8521 ttggcggcga gcaattccga gaacgcggtg gccaggaacc aggtgcccat catcaggctg
     8581 gcgacgcgcg gcaccgacaa ctgcgtgact gcggacaggc cgaccggata caggcagatc
     8641 tcacccacct gcagtaccgc atatgccagc accagccacc aaacactggc gaacgtgccg
     8701 gcatcgcctg cgccgtgcgc ggcgagatac aggggcagga aggacagcgc gccgaacagc
     8761 aggcccagca cgctcttgag tggtttgccg ggattgcggc cacgccgatc cagccagctc
     8821 cacagccacg agaacagcgg cgcgaacgag aggatgaaga tgccggaaat gaaggtaagc
     8881 gagccagccg tctgcggttg cagcaccacc atcccgacca ccatcaacag ggtcaccgcg
     8941 gcggcggcga gaaacaccag ggtgggcagg cgcgagccgg ggctgcgtga ctgccagcgc
     9001 atggccagca tgaacgccgc cggtagcagc aaggtgcaga tgacgaagta cgggaacggc
     9061 aagccctccg ccaggaccag ggacgggaag aaatccttgg tcagcaggcg atcggtgaat
     9121 accacccagg aaccgtaggt ctgctcgtac agcgtgtaga acagcagcgc gatgaagatc
     9181 aacgccatca aggtgagcat ctgcccacgt tcgcgcggcg tgcagtcgcg caggacgaag
     9241 cgcgtgaacc acgcgagcac caccgccagc acggccagca tcagcaattg cgccagggtg
     9301 agatcgacgc cgatcgacag ggtgaaggca tcgttgccgg tcgcccacat caggcccgcg
     9361 atcggcaaca agccgagcag ggtggcgaga tagatcgccc attcgcgcgg catcccggcg
     9421 acacgttcgc gcaggcgttc gggcgatggc ggttccgcca tgccgtgcag gtatttctgc
     9481 ccccacagga acatcgccag tccagccagc atgccgatgc cggcggcacc gaagccgtac
     9541 ttccagccat aggtttcgcc gaggacgccg cagaccagtg atgcgagcat cgcgcccagg
     9601 ttgatgccgg cataaaacag cgagaaaccg gaatcgcggc gcggatcgtc ttccgcatac
     9661 agcttgccga cgatcaccga gatattgggt ttgaggaaac ccacgcccat gatgatcagc
     9721 gccagcgagc cgtagaacac cagcagtgcg gcatcgtcgc ggacgatggt gtcgccgacc
     9781 ttgcgcgcct gctcgccttc caccgccatg cccaggtggc ccagtaccag cagcacgcca
     9841 ccgaacacca cggccttgcg catgcccagc caacggtccg ccagcaggcc gccgagcacg
     9901 ggaaggcaat acaccaggcc gccataggca cccagcaggt tcaggccgcc ggtgtcgctg
     9961 aacaggtggt acttcgtgag gtagagcagc agcaacgcct tcatcccgta gaaggagaag
    10021 cgctcccaca tctcggtgaa gaagcagacg tagacgccct tgggatggcc gaggaagtcg
    10081 gcgtgccgcg gcgcatcgct gaagctggga ttcatgggcg tacttcgcgc ttgggctcgc
    10141 ggaagtatag gcagtgatgc gccggcgagg cctgccgcaa ccaagcgagt gccggtgagt
    10201 gcgcccgcgc cggcgggcgc gttgcggccc gccgatgcgg cgttaccgga caggtgcgga
    10261 ctggacttga acagcgccag ccgctaggtt tcgtcgattg catgccttgg gagaggggtc
    10321 gatggcgaat tcaggttcgg gcacaccgca gttgacgttg cgtgcggtga tccttgcggt
    10381 ggtgctggcg atgatcctgt ccgccgcgaa tgcgtacctg gggctgttcg ccggcctgac
    10441 catcgccacc gcgatcccgg cggccgtggt gtccatgggt gtgctgcgcc tgctcggcgg
    10501 cggcaccatc ctggaaaaca acatcgtcca gaccggcgcg tccgcgggtt cctcgatcgc
    10561 ggcaggcgtg atcttcacga tccccgcgct gatcatcctg ggctactggc aggatttcaa
    10621 atattcctgg gtgctggcga tcgccggcct gggcggcctg ctgggcgtgc tgttctcggt
    10681 gccgctgcgg cgctcgatga tcgtcgagga cccgctgccg ttcccggaag gcaaggccgc
    10741 cgcggaagtg ctgaaggccg gcgagaaccc cggcccgggc ctgaagatcc tcggcatctc
    10801 ggcgtcgatc ggcgcactgg tcaagctggc cgcggaaagc ggtttccgca tgatcccgga
    10861 caacgcggcc ggtgccggtt tcttcggcaa gtacctgggt tacatgggca ccggcctgtc
    10921 gcctgcgctg ctcggcgtgg gctacatcgt cggcctcaac atcgggatcg tggtcgtgtc
    10981 cggcagcgtc ttcgcctaca acctggcgat cccgttctat cacgcgttcc tgctgaacgg
    11041 cgatccggaa gtcgcggcca aggtggtcgg caagaccgcg gccgaagcgg ccgcgatcat
    11101 ccgcggcgac cgcatccgct acctcggtgt gggcaccatg ctggtcggcg gcatctggac
    11161 gctgttctcg ctgcgcaatt cgctgctgtc gggcgtcaag agcggcatcg cggcggcgcg
    11221 caagggtgcc gatgcgaacc tgcccgaaac cgagcgcgac ctgccgatga agtggatgct
    11281 catcgcgctg ctgctgttcg tgctgccgct gctgggcctg taccaggcca tcgtgcagaa
    11341 ctggggtgtg agcatcccga tgacgatcat catgatcgtg gccggcttcc tgttcgtgtc
    11401 cgtctcggcg tacctcgccg gcttggtcgg ttcgtcgaac aatccggtgt cgggcatcac
    11461 catcgccacc atcctgttcg cctcggtggt attgatgctg ctgctcggca gcgactccaa
    11521 gatcggtgcg gtcgcggcga tcatgatcgg cgcggtggtg tgctgcgcgg cagcggtcgg
    11581 cggtgacaac ctgcaggacc tcaaggccgg ctacatcgtc ggcgccacgc cgtggaagca
    11641 gcagttgatg ctcggcatcg gcgcgttctc ctgcgcactg atcatggcgc cggtgctgaa
    11701 cctgctcgcc caggcttatg gcatcggcga cccgacccgc ccaggttcgc tgcaggcgcc
    11761 gcaagccacg ctcatggcct ccgtggcgca agggctgttc gggggcaagc tgccctggaa
    11821 catgatcatc gccggcggcg tgatcggtgc gatcgtcatc gccatcgacg gttggctgaa
    11881 gtcgcgcaac tcgaccttcc gcgtgccggt gctggccgcg gccatcggca tctacctgcc
    11941 gctggaactg atggtgccga tcttcctcgg cggcctgctg gcgcatttcg tcgagaagcg
    12001 ccacggcatc gtcggcaccc atgacgaagc cctgcgcgac cgcgtgcatc gccccggcgt
    12061 gctgttcgcc gccggcctga tcaccggcga ggcgctgatg ggcatcgcca tcgcgatccc
    12121 gatcgtgatg accgagcgcg ctgacgtgct cgcgctcccg gagcagttcc acttcgggca
    12181 attcgtgggc ctggtcgtgc tggccatcgt cggctggctg atctaccgca ccggcgccaa
    12241 ggcgaacccg aacgccgcct gatccatgag cgcccgcgca tcgtcgcggg cgattcttgt
    12301 tcgcagtacg cacttcaaga cacggactac cgccatgaag attcgcaacg ccctcctgcc
    12361 actcgccctt gcgctctccc tgcccgccgt cgccgatgcg acgcgcggct tcgaagtgcg
    12421 cgacatggcc tacctcgacc ggcactcttc gccgacgctg tcgccggatg ggcgcgtgct
    12481 ggtgttcgcc aagcgcgtgg tcgacaaggg caccaacaag tccagcacct cgctgtggac
    12541 gcgcaacctg ctgacccgcg acctgcgccc gccgtcgcgg ctcacgccgg aaggctggaa
    12601 cgtcaattcg ccgtcgttct ccgccgatgg caagaccgtg tacttcatga gcggcaagtc
    12661 ggggtcgatg cagctgtact cgatgccgtt ggccgggggc gagccgacgc ggctcaccga
    12721 ctacgcgctg gatgtcgatg cctacaagct gtcgcccgat ggcacgcagg tcgcgctggc
    12781 gttcgcggtg ttcccggact gcaaggcaga cctcgactgc acgaagaaga agctcgacga
    12841 cgtggacgcg ctgaagacca gcggcaaggt gttcgaccgc atgttcatcc gccactggga
    12901 tacctggaac gacggccgcc tcaaccgcgt gttcgcggcg aagctcggcg gcgctggcat
    12961 gctgaaatcc gccacgttgc tgagcggcga catcgccggc gacattccgt ccaagccgtt
    13021 cggtgacctg tccgatttcg cctggtcggc ggatggcaag cgggtggcga tgagcgtgcg
    13081 ccaggccaac cgcgaggagc cgtggagcac caacttcgac ctctggctgg tcaatgcgga
    13141 cggcagcggt gcacgcaacc tcaccgcgaa gaaccccgcc tgggatgccg gtccggtgtt
    13201 cagcgccgac ggcaagacgc tttactaccg cgcgatggcg cgccctggct ttgaggccga
    13261 tcgcttcgcg ctgatggcga tggaccttgc ctccggtcag tcgaaggaaa tcgcatcgaa
    13321 gtgggatgcc tccgccgatg gcatcaccct gtccgccgat ggcaagtgga tctacaccac
    13381 ggccggcgag ctgggccggc acccgctgtt ccgggtttcg ctggccaatg gcgaagtgga
    13441 gagcgtggtc aaggacggca gcgtgtcctc gttcgacctg gccgggcaga cgctggcgat
    13501 ctcgcgcaat tcgatgaaga gcggtgacgt gatctacacg accagcgccg acggcaaggc
    13561 tcccctgcgc gcgatcaccc cgagcgccgg cgagatgctg ccggatgtgg cctggggcga
    13621 atacgagcag ttcaacttca tcggctggaa caacgagacc gtgcacggtt acgtggtcaa
    13681 gccgtggaac taccagccgg gcaagaagta tccggtcgcg ttcctgatcc atggcggccc
    13741 gcagggcagc ttcggcgatg gctggagcta tcgatggaac ccgcagacgt acgcagggca
    13801 aggctatgcg gtggtgatga tcgatttcca cggcagcacc ggctacgggc agaaattcac
    13861 cgacgcgatc agcggccact ggggcgaccg tccgctggag gatctgcaga aggggtgggc
    13921 cgcggcgcag gagaagtacg cgttcctgga tggcgagaag gcctgcgcac tcggcgcgag
    13981 ctacggcggc ttcatggtca actggatcca gggcaactgg tacgacaagg ccggcaactc
    14041 gccgtggaag tgcctggtca gccacgatgg cgtgttcgag aacaacgcga tgggctacgc
    14101 caccgaggaa ctctggttca gcgagtggga aaacggcggc acgccattcg acaatccgaa
    14161 gggctaccag cagttcaacc cggccaacca cgtcgccaat ttcaaggtgc cgatgctggt
    14221 gatccatggc cagctggatt accgcatccc ggtcgagcag ggcatcgccc tgttcagcgc
    14281 actgcagcgc aagggcgtgg aatcgaagtt cctctatttc ccggacgaga accactgggt
    14341 gctgaagccg cagaacagcg tgcagtggca tgacacggtc aacggctggt tgaagcagta
    14401 catcggccag tgagcatgga gcctgtcgcg ggtaccgcgc tgatcaccaa cgaagcagcg
    14461 gtgctgggcc tgctggccgt catcctcggc ttcgtgttct ggggagcctc gcgcgaacgc
    14521 ggtttctgga agaagttcta cgcggtggtg cccgcgctgc tgccctgcta cctgctgccc
    14581 gcgatcgcca acagcaccgg cctgatcgac ggtgcgggat ccggcctgta cccgatggcg
    14641 cgcgactacc tgctgccggc atcgctggtg ttgttctgcg tgtcgatgga catcggtggc
    14701 atcgccaggt tggggccaaa ggcgttggtg atgttcctga ccggcacggt cggggtgatg
    14761 ctgggtgcat tggtgtcgtt caccgtgatg ggcgccctgc atcctgagac ggtcgccggt
    14821 gagacctggc gcgggatgac cacgctgacc ggaagctgga tcggcggcgg cgccaaccag
    14881 gcggcgatga aggaggtctt cgccgtcgat gccaccctgt tcggccagtt cgccgcggtg
    14941 gacgtgatcg tcggttacgt gtggatggcc gccctgctgt tcatggcctc acgcgcggag
    15001 gcgtgggatc gctggctgaa ggcggacacg tccgccatcg agacgctcaa gcagcggatg
    15061 gcgacctacc aggcgcagca tgcacgcatc gcctcgctga ccgacctggt gctgatcttc
    15121 gcggtggcga tcggtgccac gggcctggcg catttcctgg ccgagccgct ggtggcgtgg
    15181 atcaagtcga tgccgctcga gtggcgcctg caggattaca gcctggattc gcgcttcttc
    15241 tggatcgtgg tgatcgccac cgcgatcggc gtggtggcca gcctgacccg tgcgcgcgaa
    15301 ctcgagggtg ctggcgcgtc gaagatcggc acggtgttcc tgtacgtgct gatcgccacc
    15361 atcggcatgc agatggacct gaaggcactt gccgacaagc cgttcctgtt gctgctcggg
    15421 ttgatctgga tctcggtgca cgccggcctg atgctgctgg tcgcgcggct gatccgcgcg
    15481 cccttgttct tcctggcggt cggatcgcag gccaacatcg gcgcggcggc cagtgcgccg
    15541 gtggtcgcca gtgcgttcca tcccgcgctt gcgccggtgg gcgtgctgct tgcggtgctc
    15601 ggctacgtcc tgggcaccta ctgtgcctac ctgaccggat tgatgctgca ggcgctggcc
    15661 gggggctgac tcacagccag tgcaccttgc gcaggtagcg gtacaggccg gtgcagaaca
    15721 gggccacgcc gccgatcagg atccagtagc tgtagcgtcc gtgcaattcc ggcatgtgct
    15781 cgaagttcat gccgtaccag ctggcgatca gcgtgggcgc ggccagcagg ccggcccagc
    15841 cggccaggcg cttgacgatc tcgccctgcg ccacggtgac cagcgacagg ttcacgttca
    15901 tcgccgcggt gaccatctcg cgcagggtgt cgatggtgtc gccgacgcgc gcggcatggt
    15961 cgagcacgtc gcggaaatag ggcttcacct cgtcggggat ggccaggctc gggttgcgca
    16021 tcagctgcga gaggatgtcc tgcatcggcg ccaccgccat gcgcatctgg gtcagttcct
    16081 tgcgcagttc gtacagccgg cgaatggtgc cgcggcgata gctttccgcg aagacctcgt
    16141 gctccagcgc atccagttcc tgctcgaagt cggagacgat cggctggtag ttgtcgacga
    16201 tgaaatccag caccgcgtgc agcgccgcgg tcgggccctg cgccagcatc tccggctcgc
    16261 gttccagccg ctggcgcacc gaggcgaagg tcagcgacgc gccatggcgc acggtgacga
    16321 ggaagcgcgg gcccacgaac aggtgggtct cgccgaagcg cacgtgtccc tccacgcgct
    16381 gcgcggtgtg cgcagcgatg aacagcgtgc tgccatagcc ttccagcttg ggtcgctggt
    16441 gcgcgttctg ggcatcctcg atggccaggt cgtgcagccc gaattcctcc tgcagcttgt
    16501 cgagcagcgc gtcttctggc tcgtacaggc cgacccagac gaagctgcca tcgtccaccg
    16561 ccagcacgtc gctgatcgca tccaggtgga tgtcgcggcg ccggcccgcg cggtcgtagg
    16621 cggcgcagtt gacgacgcag gcgggctggt cgctgatgtg ctggtggttc atcgcggcat
    16681 cctgcatggc ggcagccccg ccatcaagcg tggtcacgcg tgcggccgcc gcgcccacgc
    16741 ccgcgcgagc gccgcatgga tcggcagcag cagggcgatc cagtggccgt tgcgttgcgg
    16801 ctgcgcttgc agccacaacg ggaggcaggc cagtgccgcc atggccgcga ggacgatgag
    16861 caccgtgcgg aaacgcgacg agggcatgcg accacgcagc aatgcccagg cgccgggtat
    16921 cagcgcgagg cacagcgggt tgagcaacag gaggttgcgg ttggcccaca gggcgtggtg
    16981 cgcggtgaag gcccagccca gcgccagcac caggcccaac agcccgcagg tcgcccagaa
    17041 agcgcccgcg aatcccgcaa gcaggcgtgg acgccagcca gcgagcacca gcactgccgt
    17101 gcctgccagg acgccggcca gcagccaggg ccacaaccgg ggtgcacggc cgaccggttc
    17161 cgcggcctgt cgttgcggca gcagttcgat ctcctccgcg accagtgggc gaccatcggc
    17221 gcgtgtcgct tcgcggaggt cgtcggctaa ccgacgcggc aggaaggcct gctgccagcg
    17281 ggtcagcggc cggtccgcga acggccccag gcccagatcg aagccgaccc gcatccacgc
    17341 ggccggcgag gccagccgca ccgattcggt gcgataggta tcgccgctgg aacgcacgtc
    17401 cagctgtcgc cgcagttgcc cgccgagcgc acggtcgatc gcgtcgcgca cccggctggc
    17461 gcagttgtcg gtgtagtagt cgtaacggta gcgggcgttc tcgggtttcg cgttctccgc
    17521 cagcgcggca gcgagcgaac gtgcctgcgc gggatccagg tccagccact gcaggcgcgc
    17581 gccgcggccg acctcgcggt agtagcgcag gtcgtcctcc agcggcagcg cgaccagcat
    17641 gtattccatc tcgccgcgca cgaagcggcc gatgaagccg tcctcggcca ggtcgaagaa
    17701 cccgaagttg tagctggtcg gccctgcggg tgcgccgtcg tcgaccacga tcgcgtcatg
    17761 gccgaaacgt tcccagaaga cgtcgcccgg acccatcgtc acaacgccga tgcggggcgc
    17821 gtccgacgct ggcgcgagca ccggcgaagc ggatccggag ggctcgacgg attgcgcgtg
    17881 cgccagtccc agcgcgcacg cgagcaacag cgcggtggcg acgcgcagcg cgtcagtgat
    17941 ccgcatgcac cgtcacgtgc aggctcagca agcgtcgtgc gtccgcacgc gcgacgcgga
    18001 agccgaagcg gcccaaggcc agttcctccc cgacctcggg caggtggccg acggcatcgg
    18061 tgaccaggcc gccgatggtg tcgtattcgt cgtcgtcgaa gtcggcgccg aaacgcgcgt
    18121 tgaagtcgtc gatcggggtc agcgcatcca ccatgaactg gccgtcgtcc tgcgccgcga
    18181 tcagccgcga atcgtcctcg gcctcgtcgt gctcgtcgtc gatgtcgccg acgatctgct
    18241 ccagcacgtc ctcgatggtc accaggccgg cgacgccgcc gtattcgtcg atgacgatgg
    18301 ccatgtggtt gcgcgactgg cggaactcgc gcagcagcac gttgagcttc ttggattccg
    18361 ggatcagcac cgccgggcgc agcagttcgc ggatggtgcc cggcccgttg tcggcgacca
    18421 cgccgcgcag caggtccttg gccagcagga tgcccaggat ctcgtccttg tcctcgccat
    18481 gcaccgggaa gcgcgagtgg ccggattcga ccacctgttt catcaggtcc aggaacttgc
    18541 cgtcggccgg cagcgcgacc atctgcgcgc gcgacaccat gacgtcgccg acggtgaggt
    18601 cggccaccgc gatcgcgccc tccatcatcc gcagggtgtc ggcggcgatc aggccgtcgg
    18661 cctgcacgtc gcgcagcaac tcgaccaggt cctcgcgcgt ggtgggctcg cccgacaggg
    18721 cggcactgat gcgatccagc caggaacggc gtggtttgtc gccgctgcgt tcagcggaat
    18781 cggcgtcttg ccgactgtcg tcctcggaca tggtgcgcgg ggttgcctgt gggtgtaccc
    18841 ggcgagtcta gcgaatcggg gcggcatggc ggtgaatcgg gcgcgccggg gcggcggcat
    18901 aggctgcccg tccggttgcg tggccatggg ccattgcgga cactggcggc ggggagccgg
    18961 catcgcgccg gccgtgcacg gggtgaagct tggcaacaga cgacggcctc ctgatcaagc
    19021 cggaaccgcg caaaccctgg gtcgtcgtgg ccgttgccgc cggcgtgctg gcggtgctgg
    19081 caggcgggta cgtcgcctcc ggactgtccg gcaagccgaa acccgccgcc accgcgacgg
    19141 ccgcatcgcc ggcgccggtc accgccaacc ccgaccaact gcctgcgatc atcatcgcgg
    19201 tgctgccgtt ggccgccgac gcgggagcga ccgcggcacc cgccgacggg gacgcctccc
    19261 agcacctggt cgatgcgctc aagggcggca gcgagcgcgg gtcggccttc aacgacggcg
    19321 tgtccgcgca gctggccggt gccttgtcgc aattcgacgg ggtggccgtg accagcccgg
    19381 agtcgtcctt ccagcagcgc gatgcggatg cgccgccggc gacgatcggc aagatgctgg
    19441 gtgctaccca cctgctgcgg ggcacggcca gccgcgacgg cgagacgctg cggatcgatg
    19501 ccgcgctggt cagcgtcgcg gacggcagca cggtgtggtt cgaccgctac cagcgtccgt
    19561 acgccgagtt gttcaaggtg caggacgaga tggctgcggc gatcggcgca gcgttgaagg
    19621 tcaagcgctt gccggcgccg cagggcgggc aggacgagcg gccgccgggc gggagcctgc
    19681 aggcgtacga agcgctgctg gcgggcgacg cggcgctcga gcgcgccgat gcggacggcg
    19741 tggcggcggc
//
LOCUS       DAOOCX010000039        19643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2442_length_19643_cov_7.217225, whole genome shotgun sequence.
ACCESSION   DAOOCX010000039 DAOOCX010000000
VERSION     DAOOCX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 19643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19643
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2442_length_19643_cov_7.217225"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PK399_05500"
     CDS             complement(<1..870)
                     /locus_tag="PK399_05500"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPW12607.1"
                     /translation="MSKQVQDAYIVAATRTPVGKAPKGVFRNTRPDDMLAHVLKSVVA
                     QAPGIDLNRIDDAIIGCAMPEGEQGMNVARIGLLLAGLPNTIAAQTINRFCSSGLQAV
                     AMAADQIRLGNADLMLAGGTESMSMVPMMGNKIAMAPSVFDNDHVAIAYGMGITAEKV
                     AEEWKVSREDQDAFAVASHQKALAAQAAGEFRDEISAYEVISRQPDLAGNAVRLKKLL
                     IEHDEGPRADTSLEGLAKLRTVFRNQQFGGTVTAGTASQMSDGAAGVLLASEQAIKDY
                     GLTPLARFVSFSVA"
     gene            complement(894..3266)
                     /locus_tag="PK399_05505"
     CDS             complement(894..3266)
                     /locus_tag="PK399_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HPW12608.1"
                     /translation="MSQKLHVRRAAVLGAGVMGAQIAAHLVNAGVDTVLFDLPAKEGD
                     PNGVVTKAIAGLAKLSPAPLASKSLGEAITAANYETGLEQLRDCDLVIEAIAERMDWK
                     QDLYRKIAPFIPAHTVLASNTSGLGINTLAEVLPEELRHRFCGVHFFNPPRYMHLAEL
                     IPAKSTDPEVLEGLDTFLTSTLGKGVVYARDTPNFIGNRIGVFSILAAIHHTAAFKLG
                     FDEVDAISGPLVGRPKSATYRTSDVVGLDTMAHVIKTMADTLPDDPWHPYFKSPAWLQ
                     ALIGKGALGQKTGAGIFRKAGKDILVVDVEKQDYRPADRAAAPEVVEILKTRNPAEKF
                     QKLRESQHPQAQFLWATFRDLFHYSAYHLADIAETARDVDLAIRWGYGWSLGPFETWQ
                     AAGWKQVAQWIADDIVAGKAMSSAPLPDWVFDGREGVHAAEGSFSPAKNAKLPRSTLP
                     VYKRQRFPDPLLGEVFAPGETVYENDGIRLWTDGDDIAVASFKTKMHTVSDAVLDGLQ
                     EAIGIAERTFKGMVIWQPKEPFSAGADLSGALAALQAGKLAEFEAMVANFQATSQRIK
                     YSLVPVVAAVRGLALGGGCEFQMHSARSVAHLESYIGLVEAGVGLLPAGGGLKEIAVR
                     ASQAAGPGGDVFAELKKTFETVAMAKVSASAVEAKELGLVRANDKVVFNAYELLHIAR
                     QEAAALAETGYRPPLPARRIQVAGDVGVATFKMMLVNMLEGRFISPYDYEIAVRIATV
                     LCGGEIDRGSLVDEDWLLKLERQHFVELAQQEKTQARIAHMLKTGKPLRN"
     gene            complement(3303..3929)
                     /locus_tag="PK399_05510"
     CDS             complement(3303..3929)
                     /locus_tag="PK399_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017118749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW12609.1"
                     /translation="MTLPHFSTKDRILYAAEELFAQHGFASTSLRQVTSRADVNIAAV
                     NYHFGSKENLVNEVFRRRMDDMSRERLAALQRAVEAHPGELEPILAAFVEPALAMAQD
                     RHGGGAFIRVIARAYAESNDSLRKFLSDQYGHVLREFAKALAGCVPGLGKEALYWRLD
                     FLSGALTYAMADFGLIKRPAGVSEATHRQRAAKALIRFAAAGFQADAS"
     gene            4142..4567
                     /gene="ndk"
                     /locus_tag="PK399_05515"
     CDS             4142..4567
                     /gene="ndk"
                     /locus_tag="PK399_05515"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPW12610.1"
                     /translation="MALERTLSIIKPDAVAKNVIGDIYSRFEKAGLKVVAAKMKHLSK
                     QEAEGFYAVHRERPFFSALVNFMISGPVMIQALEGDNAVLKHRDLMGATNPKDAAPGT
                     IRADFADSIDANAVHGSDSLENAAIEIAYFFPSTDVYTR"
     gene            4575..5804
                     /gene="rlmN"
                     /locus_tag="PK399_05520"
     CDS             4575..5804
                     /gene="rlmN"
                     /locus_tag="PK399_05520"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPW12611.1"
                     /translation="MSDTRTPAIDIALLGDATGVRAPAVAAPAAAASRTNIFDFDRAS
                     LERFFEEELGEKKFRAQQVMKWIHHRYVTDFGDMTDLGKVLRAKLEARAVVHAPQVVL
                     DKASEDGTHKWLLGMDAKNAIETVYIPDKGRGTLCVSSQVGCALNCQFCSTATQGFNR
                     NLSTAEIIGQVWVAARHLGNVPHQQRKLTNVVMMGMGEPLANFENVVRAMSIMRDDLG
                     YGLANKRVTLSTAGMVPMIDKLGEVSDVSLAVSLHAPFDDLRSELVPLNKKYPVADLM
                     DACVRYALRKRGTSVTFEYTLMKDVNDQPEHARGLVKLLREFDRRVQMKDAAKVNLIP
                     FNPFPGTRFARPTDEAIRAFQKQLNNAGMIAPVRRTRGDDIDAACGQLKGQVMDRTRR
                     SAEHRKRLVAAGVSDAA"
     gene            5794..6606
                     /locus_tag="PK399_05525"
     CDS             5794..6606
                     /locus_tag="PK399_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW12612.1"
                     /translation="MRRELPVLVVAACLLASGCSKLERLTIIRPSAERGEYTKTSPTY
                     DVSGANARSRTTDPTQLLMSASDLYLKGDLAQAESLAQRALKAEPRSGDAHTLLGAIA
                     NARGQAAAAGVHYAKAVEIAPKTGIYANNYGTWLCANDQAMASLAWFDVAIADPAYPT
                     RAAALANAGSCARQAGQLERAEASWRQALAFDPGNVAALSGMATLQFSRNQYLDARAF
                     TERWLAAAPDDVDGLGLAIQVEQKLGDNAAASRYLSRLQAIPSGSGPAPRTQ"
     gene            6603..7421
                     /locus_tag="PK399_05530"
     CDS             6603..7421
                     /locus_tag="PK399_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012445040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HPW12613.1"
                     /translation="MTSKLAHPDDTHQTLAGCGERLRLAREASGLSVDDVAARLHMPA
                     RVVKSLEAEDWSRLGAPVFVRGQVRSYSRLLGLTTAPMMAALDVGPVEPSTLVSRTHT
                     PKAQWWAEQIGRRLVYIILTLSLAVPAWVATRQHLSGASAGTTPLDAPTDPSARLPGA
                     SEPASPRTVVASMAPVATPAMQAAPVGDIVLRTSAESWLDVVAPDGASIEKGLLPAGS
                     ERRYAATQVARLTIGNASAAQLEHRGRTVDLAPFARANVARFAVSSDGSLVAAD"
     gene            7497..8141
                     /locus_tag="PK399_05535"
     CDS             7497..8141
                     /locus_tag="PK399_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW12614.1"
                     /translation="MAIDDLLNEHEQSERVRGWIRSNAIGLIGGVGLGLGAIFGWQWW
                     QAQRTQAQMGVNARYAQAIDAYEAGTLPADKGRDTIAALGKGNPTLGTLAALQLAKVQ
                     AEAGKRDDAIATLRNLRDVDADLRPIVRQRLARLLLDASKPKEALPLLDDDKDPSMLE
                     VRGDARFALGERALAQKDYLKALALVDVGAPEHRLLTLKLIEAGGTPPHTEDNT"
     gene            8141..9322
                     /gene="bamB"
                     /locus_tag="PK399_05540"
     CDS             8141..9322
                     /gene="bamB"
                     /locus_tag="PK399_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HPW12615.1"
                     /translation="MIHASHRSLRIVAATALAVAVLSGCSTIKNVFDGRGKDKSTEPT
                     ALVQISPSVSVSKLWSASLGKGEDKLGIGQHPTIVDGRVYAAAIEGGVSAFDLRTGQA
                     LWRYASELPLSGGPGAGEGLVVVGSLEGDVIALDAATGAEKWKSKVSNEVIAAPAVGG
                     GLVFVHSNDGRVTALDAATGERRWFYSADIPALTVRGTGGMTVGPGILFVGNDNGTLV
                     ALSTTDGSPVWRTPVAEPEGRSELDRMADVDGEAVLENTTLYATSHKNHTVAIDGPSG
                     QVMWDRGNGGARGLGVSNSAVVVTDPTGNVFGLDKNTGGALWQQSGLAYRNVSAPAIQ
                     GDFAVVGDLEGVLHWLRLNDGAFAARSSLGGAITGKPVVSDGILVVQTTEGQLAAFAL
                     Q"
     gene            9447..10847
                     /gene="der"
                     /locus_tag="PK399_05545"
     CDS             9447..10847
                     /gene="der"
                     /locus_tag="PK399_05545"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPW12616.1"
                     /translation="MLPLVALVGRPNVGKSTLFNALTRTRDALVHDEPGVTRDRNYGV
                     CRLVEGRPFVLVDTGGIAGEDEGLAGATARQSRAAAEEADLILFIVDGREGASALDDD
                     ILRWLRKLSKPTLLIVNKADGIDLQSALSEFARYGLGDALPTSSAHRTGIDEVLARVM
                     ERLPQEGSTETLDTDPTRVRIAFVGRPNVGKSTLVNRILGEERMIASEVPGTTRDSIA
                     VDLEREGRKYRLVDTAGLRRRAKVEEAVEKFSVFKTLQAIEQCQVAVLMLDASEGVTD
                     QDATVLGAVLDAGKALVIAINKWDGRTDYERQQAETLVARKLSFVEWAENVRISALHG
                     SGLRELFRAIHRAHASATRVFSTADVTRAIEAAYEANPPPVVRGHVPKMRFAHPGAES
                     PPTFIVHGTRLKSLSNTYQRYLENFFRKRFKLVGTPIRFLFREGENPYKDKKNVLTDR
                     QVAKKQRLIRRVKRAK"
     gene            10853..11428
                     /locus_tag="PK399_05550"
     CDS             10853..11428
                     /locus_tag="PK399_05550"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009602295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPW12617.1"
                     /translation="MIPRGDLTLGILAGGRASRLGGRDKAWLERGGTAQVTRIARRFD
                     GECATVLVSANRDLPRYAAHGLATVVDRIPGIGPLGGLEALASACATPWLLTVPVDVV
                     DTNDCLLRTLASAGGEGAVAEDDDGLQPLVALYRLAPLRDAVDAAITANALSIQAMQA
                     RIRLPRVRFSGFRFGNLNTPEDLRLAGYADD"
     gene            11421..12635
                     /locus_tag="PK399_05555"
     CDS             11421..12635
                     /locus_tag="PK399_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPW12618.1"
                     /translation="MTDFPVSILFDEARDILRRVAAAHRLPVEQVALARAHGRVLAHD
                     IVAPIALQPFDNSAMDGYACRHADLREDDAVRLTLVGEQFAGRALGLQVGAGECVRIT
                     TGAPMPAGADTVVMREDTQVDADAVTMPAGIRPGANVRKAGEDARPGDPVLRAGTRLN
                     AVHVSLAASLGLERLPVSARPTVAVFTTGDELVEPGLPLQPGEIYNSNRELLMGLLRA
                     EGLEPVAWPTLPDDPLRMASMLQDAASSFDVVITCGAVSAGEKDHIPAMLRIHGAVHF
                     WKVRMKPGMPLLAGRLANAQFLGLPGNPVSVLATFLTLGRVLIDGMQGRAGQPPRFAR
                     LQVGMEKAHPRREFVRGTLQVDETGTQWVIPNAATGSHRLRASADADALLVLAEGAQS
                     LARGDVVEVLTY"
     gene            12653..13786
                     /gene="moeB"
                     /locus_tag="PK399_05560"
     CDS             12653..13786
                     /gene="moeB"
                     /locus_tag="PK399_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HPW12619.1"
                     /translation="MQIRILSPNEAHARQRAGAVLVDVRAAHERALGMAVDASGIVRE
                     ALQADPTAHLPDPGMEILLICQRGARSLQVAEILAARGYRNLSSVEGGTDAWDAAGLP
                     MQRADVDADFSERYSRHLRLPDVGLQGQQALEAARVLLVGAGGLGSPAAYYLAAAGIG
                     TLRIADDDVVDRSNLQRQILHTDARIGMPKVESATLALSALNPRTRIEAVRERITSDN
                     VERLLDGIDVVVDGADNFPARYLLNDACVKLGKPLVYGAVHRFEGQASVFDAGRHRGS
                     APCYRCLFPEPPPPESAPNCAEAGVLGVLPGVIGLLQATEAIKLLLGIGDPLRGRLLQ
                     FDALAMRFRETRLLPDPQCPVCAPGCAFPGYVDYAAFCAGGEA"
     gene            13786..14859
                     /locus_tag="PK399_05565"
     CDS             13786..14859
                     /locus_tag="PK399_05565"
                     /inference="COORDINATES: protein motif:HMM:NF021541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2272 domain-containing protein"
                     /protein_id="HPW12620.1"
                     /translation="MVVETKRAGGLSRRWRWLAAVALLLSSAWAMAADQCPALRDRQG
                     DTEVATRIAAIACDEHLRWNRPFIDADGRLASLAVYEAESRGLQDGGSPWRRVAFYWQ
                     STGLLSPMAAKSGATDCNYAINSSYPGLGCRGFIVDNPWSAAFITWVMQRAGVPGFVS
                     SASHFDYVRAARTNPARSPYVFLDPMAAAPAVGDLLCYVRTNRVYGHRGLASAIDGGT
                     TGLAMHCDAVVAVNPGGDGKAYAIGGNVQQAVTMRQFNLNASGQLWGVPQRTEGDVEC
                     SPDTQSACNFNRQDWAVLLKLKSQAELALIGPVQPPTYMPNQVVPQACCVNCVVGSGV
                     PRCPAPGQPPLQPQPQGQLQGSE"
     gene            complement(14863..15069)
                     /locus_tag="PK399_05570"
     CDS             complement(14863..15069)
                     /locus_tag="PK399_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12621.1"
                     /translation="MAMIHGECPKCGKRVLHVNIENVVGVADGTSNARCLSYSCIHCH
                     VVLGVQVDHRAKPRSRAKPAATTP"
     gene            complement(15110..15412)
                     /locus_tag="PK399_05575"
     CDS             complement(15110..15412)
                     /locus_tag="PK399_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HPW12622.1"
                     /translation="MTAPVDNTALEAATFRRLRDHLMVERPDVQNIDLMILAGFCRNC
                     LADWYREAAEASGQPMTRDEAREAVYGMPFADFKARHQAEATPEQLAAFEARTRSS"
     gene            15467..16330
                     /gene="folD"
                     /locus_tag="PK399_05580"
     CDS             15467..16330
                     /gene="folD"
                     /locus_tag="PK399_05580"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPW12623.1"
                     /translation="MPAKILDGKRIADDLLLTLKAQVDARVAAGKSRPGLAVVLVGQD
                     PASQSYVRNKRRAAEKVGIEALDFDLPAGTTEAELLALVDRLNADPKVHGILIQLPLP
                     GIRDATALIERIHPDKDVDGFHPENVGHLALRQFGLRPCTPRGITTLLAYTDKPVRGQ
                     SATIVGVSNHVGRPMALELLIAGCTVTSCHKFTPLDVLKRHVGEADILVVAVGRPGLI
                     PGDWIKPGAVVIDVGINRLDDGRLVGDVGFAEAATRASWITPVPGGVGPMTVATLMQN
                     TLEAAELADSR"
     gene            16515..17975
                     /gene="guaB"
                     /locus_tag="PK399_05585"
     CDS             16515..17975
                     /gene="guaB"
                     /locus_tag="PK399_05585"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002805758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPW12624.1"
                     /translation="MLRIQAEALTYDDVSLVPAHSTILPKDVTLTTRLTKGLSLRLPI
                     VSAAMDTVSESRLAIALAQLGGISIIHKNMSAEAQAAQVARVKNFEAGVIRDPFTVGP
                     DATIGEVLRLTRARNISGVPVVDESGQLVGIVTGRDMRFEKKLDDPVHHIMTKKDRLV
                     TVKEGASDDEVIGLLHKHRIEKVLVVSDDFALRGLITVKDIQKKSDNPNAAYDSRERL
                     LVGAAVGVGGDTEQRIEALVAAGVDVVIVDTAHGHSQGVIDRVAWAKKRFPSLQVVGG
                     NIVTGDAALALMDAGADAVKVGVGPGSICTTRIVAGVGVPQVTAVSMVAEALQDRIPL
                     IADGGIRYSGDIGKAIVAGASSVMIGGLFAGTEESPGETELFQGRSYKSYRGMGSLGA
                     MEKGSKDRYFQDASDADKLVPEGIEGRVPYRGPLSGVVHQLVGGLRATMGYVGCATIE
                     EMRTKPSFVQVTGAGQRESHVHDVQITKEPPNYRAS"
     gene            18139..>19643
                     /gene="guaA"
                     /locus_tag="PK399_05590"
     CDS             18139..>19643
                     /gene="guaA"
                     /locus_tag="PK399_05590"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPW12625.1"
                     /translation="MTNIHNDKILILDFGAQYTQLIARRIREIGVYCEIWAWDHDPAE
                     IAAFGAKGIILSGGPESTTEAGSPRAPQQVFESGVPLLGICYGMQTMAMQQGGMVEGG
                     HHREFGYAEVEVTTPSALLDGLQDKPGLPARLDVWMSHGDRVTALPSGFNVIARTAGV
                     PIIAMADEARRWYGVQFHPEVTHTSSGELMLRRFVVDICGCRTLWTAANIIEDQIARV
                     RAQVGDDEVVLGLSGGVDSSVVAALLHRAIGDRLTCVFVDTGLLRWNEGDQVMAMFAQ
                     NMGVKVLRVNAADRYFDRLRGIEDPEAKRKVIGNLFVEIFDEESQKLANAKWLAQGTI
                     YPDVIESAGSKTGKAHVIKSHHNVGGLPEHMKLGLVEPLRELFKDEVRRLGIALGLPR
                     DMVYRHPFPGPGLGVRILGEVKREYAELLAQADHIFIDELRKAGLYDRTSQAFAVFLP
                     VKSVGVVGDARAYEWVIALRAVETIDFMTAHWAHLPYEFLGTVSNRIINELR"
BASE COUNT         3153 a         6607 c         6704 g         3179 t
ORIGIN      
        1 ggccaccgag aaactgacga agcgggcgag cggggtcagg ccgtagtcct tgatcgcctg
       61 ttccgacgcc agcaagacgc cggctgcgcc atcgctcatc tgcgacgccg tacccgcagt
      121 caccgtgccg ccgaactgct ggttgcggaa cacggtgcgc agcttggcca gcccctccag
      181 cgaggtgtcc gcgcggggcc cctcgtcgtg ttcgatcaac agcttcttga ggcgcaccgc
      241 gttgccggcc aggtcgggct ggcgcgagat cacttcgtag gcactaatct cgtcgcggaa
      301 ctcgccagcc gcctgcgcgg ccaacgcctt ctggtgcgac gccacggcga acgcgtcctg
      361 gtcttcgcgc gagaccttcc attcctccgc gaccttctcg gcggtgatgc ccatgccgta
      421 ggcgatcgcc acgtggtcgt tgtcgaacac gctgggcgcc atcgcgatct tgttgcccat
      481 cattggcacc atcgacatcg attcggtgcc gccggccaac atcaggtcgg cattgcccag
      541 tcggatctgg tcggcggcca ttgccaccgc ctgcaggccg gacgaacaga agcgattgat
      601 ggtctgcgcg gcgatggtat tcggcaggcc ggccagcagc aggccgatgc gcgccacgtt
      661 catgccttgc tcgccctccg gcatcgcgca gccgatgatg gcgtcgtcga tccggttgag
      721 gtcgatgccc ggcgcctgcg ccaccaccga tttcagcacg tgggccagca tgtcgtcggg
      781 acgggtgttg cggaacacgc ccttgggtgc cttgccgacc ggggtgcggg tggcggcgac
      841 gatgtaggcg tcttgtactt gcttgctcat ttgtttgcgt cctttgtcgt cgctcagtta
      901 cgcagcggct tgccggtctt cagcatgtgg gcgatgcggg cctgggtttt ttcctgctgg
      961 gccagttcca cgaagtgctg gcgctcgagc ttgagcagcc agtcctcgtc gaccagcgat
     1021 ccgcggtcga tctcgccgcc gcacagcacg gtggcgatgc gcacggcgat ctcgtagtcg
     1081 tacgggctga tgaagcggcc ctccagcatg ttcaccagca tcatcttgaa ggtggcgacg
     1141 ccgacgtcgc cggccacctg gatgcgccgc gccggcagtg gcggacggta gccggtctcc
     1201 gccagcgccg cagcttcctg cctggcgatg tgcagcagtt cgtacgcgtt gaacacgacc
     1261 ttgtcgttcg cgcgcaccag gcccagttcc ttcgcttcca ccgcggaagc cgacaccttg
     1321 gccatcgcca cggtctcgaa ggtcttcttc agttcggcga acacgtcgcc gcccgggccc
     1381 gccgcctgcg aggcgcgtac cgcgatttcc ttgaggccgc caccggccgg cagcaagccg
     1441 acgccggcct cgaccaggcc gatgtagctc tccaggtgcg ccacgctgcg ggcgctgtgc
     1501 atctggaact cgcagccacc gcccagcgcc aggccgcgca ccgccgccac caccggcacc
     1561 agcgagtact tgatgcgctg gctggtggcc tggaagttgg caaccatcgc ctcgaattcg
     1621 gccagcttgc cggcctggag cgcggccagc gcaccggaca ggtcagcacc ggcagagaac
     1681 ggctccttcg gttgccagat gaccatgccc ttgaacgtgc gttcggcaat gccgatcgcc
     1741 tcctgcaggc cgtccagcac ggcgtccgac accgtgtgca tcttggtctt gaagctggcc
     1801 accgcgatgt cgtcgccgtc ggtccacagg cggatgccgt cgttctcgta caccgtctcg
     1861 ccgggtgcga acacctcgcc cagcagcggg tcggggaagc gctggcgctt gtagacgggc
     1921 agggtggagc gcgggagctt ggcgttcttc gccgggctga agctcccctc ggcggcgtgc
     1981 acgccctcgc gaccgtcgaa tacccaatcc ggcagcggtg cgctgctcat cgccttgccg
     2041 gccacgatgt cgtcggcgat ccattgcgcg acctgcttcc agccggcggc ctgccaggtc
     2101 tcgaacgggc ccagcgacca gccgtaaccc cagcggatcg ccaggtccac gtcgcgcgcg
     2161 gtttcggcga tgtcggccag gtggtaggcg ctgtaatgga acaggtcgcg gaatgtcgcc
     2221 cacaggaact gcgcctgcgg atgctggctc tcgcgcagct tctggaattt ctcggcagga
     2281 ttgcgggtct tgaggatctc gaccacttcc ggcgcggcgg cgcggtcggc cggacggtaa
     2341 tcctgcttct cgacatcgac caccaggatg tccttgccgg ccttgcggaa gatgccggcg
     2401 ccggtcttct ggcccagcgc gcccttgccg atcagcgcct gcagccacgc cggcgatttg
     2461 aaatacgggt gccacggatc gtcgggcagg gtatcggcca tggtcttgat gacgtgggcc
     2521 atggtgtcca ggccgaccac gtccgaggtg cggtaggtcg ccgacttggg ccgccccacc
     2581 agcggtccgc tgattgcgtc tacttcgtcg aagccgagct tgaacgccgc ggtgtggtgg
     2641 atcgcggcca ggatggagaa caccccgatc cggttgccga tgaagttggg ggtgtcccgc
     2701 gcgtagacca cgcccttgcc gagcgtgctg gtcaggaagg tatcgaggcc ttccaggacc
     2761 tcgggatcgg tggatttggc cgggatcagt tcggccaggt gcatgtagcg gggcgggttg
     2821 aagaagtgca cgccgcagaa gcgatgccga agttcttcgg gaagaacttc agccaacgtg
     2881 ttgattccca agcctgaagt gttgctggcg agtaccgtat gggcgggaat gaacggggcg
     2941 atcttcctgt acaggtcctg cttccagtcc atccgctcgg cgatggcttc gatcaccaag
     3001 tcgcagtccc gcagttgctc aagccccgtc tcgtagttgg cggcggtgat ggcctccccc
     3061 agcgacttgc tcgccagcgg cgcggggctg agtttggcca ggccggcaat cgccttggtg
     3121 accaccccat tggggtcgcc ttccttggct ggcaggtcga agaggacggt gtccacgccg
     3181 gcattgacca ggtgcgcagc gatctgcgca cccatgacgc cggcaccgag cacggcggca
     3241 cgacgtacgt gcagtttctg agacatgtgg ggtatctcct tgataatgct aatggaaaag
     3301 tgttagctgg cgtcggcctg gaagccggct gcggcgaaac ggatgagcgc cttggcggcg
     3361 cgctggcgat gggtggcttc ggacacgccc gcgggtcgct tgatcaggcc gaagtcggcc
     3421 atcgcatagg tcagcgcgcc ggacaggaag tccagccgcc agtacagggc ttccttgcca
     3481 agccccggca cgcacccggc cagggccttg gcgaactcgc gcaggacgtg gccgtactgg
     3541 tcggacagga acttgcgcag gctgtcgttg ctttcggcgt aggcgcgggc gatcacccgg
     3601 atgaacgcgc caccgccgtg gcggtcctgg gccatcgcca gcgcgggctc gacgaaggcg
     3661 gccaggatcg gctccaactc cccgggatgc gcctccaccg cgcgctgcag ggcggccagg
     3721 cgctcgcggc tcatgtcgtc catgcgtcgg cggaacacct cgttgaccag gttttccttg
     3781 ctgccgaagt ggtaattgac cgccgcgatg ttgacatcgg cgcggctcgt gacctgtcgt
     3841 agagacgtcg atgcaaagcc atgttgcgcg aacagttcct ccgctgcgta caggatccgg
     3901 tccttggtcg agaaatgcgg cagggtcatc ggtcacctcg tcgaatcaaa cgcttgtttg
     3961 atgctacggc cggtgcgcca cggccgtcaa tcgccgggca tcggaaagat gcggactgtc
     4021 ggtgaaatcg gctagaattc gccgtagcca tatcggccaa acccgcgcca gtacgcgggt
     4081 ttttctgtta tcttcgggct tgcgacctgt cggtttgcgg cccgcctcac tggagaatcc
     4141 catggcgctg gagcgcaccc tttccatcat caagcccgat gccgtcgcca agaacgtcat
     4201 cggcgatatc tattcccgct tcgagaaggc cggcctgaag gtcgtcgcgg ccaagatgaa
     4261 gcacctgtcc aagcaggaag cggaaggctt ctacgccgtg caccgcgagc gcccgttctt
     4321 cagcgcgctg gtcaacttca tgatctccgg cccggtgatg atccaggcgc tcgagggcga
     4381 caacgccgtg ctcaagcacc gcgacctgat gggcgccacc aatccgaagg atgccgcgcc
     4441 gggcaccatc cgcgccgact tcgccgacag catcgacgcg aacgcggtgc acggttcgga
     4501 ttcgctggag aacgcggcga tcgagatcgc ctatttcttc ccgtcgaccg acgtctacac
     4561 gcgctgatcc ggcgatgagc gatacgcgca ccccggccat cgacatcgcc ttgctgggtg
     4621 atgccacggg cgtgcgcgcc ccggcggtgg cggctccggc cgccgccgcg tcgaggacca
     4681 atatcttcga tttcgaccgc gcctcgctcg agcgcttctt cgaggaagag ctgggcgaga
     4741 agaaattccg tgcgcagcag gtgatgaagt ggatccatca ccgctacgtg accgacttcg
     4801 gcgacatgac cgatctcggc aaggtgctcc gcgccaagct cgaagcgcgc gccgtcgtgc
     4861 atgcgccgca ggtggtgctc gacaaggcgt ccgaagacgg cacccacaag tggctgctgg
     4921 gcatggacgc gaagaacgcg atcgagaccg tgtacatccc ggacaagggc cgcggaacgc
     4981 tgtgcgtgtc gtcgcaggtg ggttgcgcac tcaactgcca gttctgctcg accgcgaccc
     5041 agggcttcaa ccgcaatctt tcgacggccg agatcatcgg ccaggtctgg gtggccgcgc
     5101 gccacctggg caacgtgccg caccagcagc gcaagctcac caacgtggtg atgatgggca
     5161 tgggcgagcc cctcgccaat ttcgagaacg tggtgcgcgc gatgagcatc atgcgcgacg
     5221 acctcggcta cggcctggcg aacaagcggg tcaccctgtc gaccgccggc atggtgccga
     5281 tgatcgacaa gctgggcgag gtcagcgacg tgtcgctggc ggtgtcgctg catgcgccgt
     5341 tcgatgacct gcgcagcgaa ctggtgccgt tgaacaagaa gtacccggtc gccgacctga
     5401 tggatgcctg cgtgcgctat gcgctgcgca agcgcggcac ctcggtcacg ttcgaataca
     5461 cgttgatgaa ggacgtcaac gaccagcccg agcacgcgcg cggtctggtc aagctgctgc
     5521 gcgagttcga tcgccgcgtg cagatgaagg acgccgccaa ggtcaacctg attccgttca
     5581 acccgttccc cggcacgcgt ttcgcgcgac cgacggacga ggcgatccgc gcattccaga
     5641 aacagctcaa caacgcgggc atgatcgcgc cggtacgccg cacccgtggt gacgacatcg
     5701 atgccgcctg cggccagttg aaggggcagg tgatggatcg caccaggcgt tccgccgaac
     5761 accgcaagcg gcttgtcgcg gccggggttt ccgatgcggc gtgaacttcc ggtgctcgtg
     5821 gtagctgcct gcctgctggc ctccggctgc agcaagctgg agcggctgac catcatccgc
     5881 ccgtcggcgg agcgcggcga atacaccaag acctcgccca cctacgacgt cagtggcgcg
     5941 aatgcccgtt cgcgcaccac cgatcccacc cagttgctga tgtcggccag cgatctctac
     6001 ctgaaaggcg atcttgcaca ggccgaatcg ttggcgcagc gtgcgctcaa ggcagaaccg
     6061 cggtctggag atgcccatac cctgctcggc gcgatcgcga atgcccgcgg ccaggcagcc
     6121 gctgccggcg tccattacgc caaggcggtg gagatcgccc ccaagaccgg gatctacgcc
     6181 aacaactacg gaacctggtt gtgcgccaac gaccaggcga tggcgtcgct ggcctggttc
     6241 gatgtcgcca tcgccgatcc tgcctatccc acccgcgccg cggcactggc caatgccggc
     6301 agttgtgccc gccaggccgg tcaactcgaa cgcgcagagg cgagctggcg gcaggcgttg
     6361 gcgttcgacc ctggcaacgt ggcggccttg tccggcatgg caacgctgca gttctcgcgc
     6421 aaccaatacc tcgatgcacg tgcgttcacc gaacgctggc tggccgccgc acccgatgat
     6481 gtggatggcc tgggcctggc gatccaggtg gaacagaaac ttggcgacaa tgcggccgct
     6541 tcgcgctatc tttcgcgttt gcaggcgatc ccctccggtt ccggcccggc cccgcgcacg
     6601 caatgacctc gaaactcgcc catcccgacg acacccacca gacccttgcc gggtgcgggg
     6661 agcgtttgcg cctggcgcgc gaggcgtcgg ggctgagcgt ggacgacgtg gcggcgcgcc
     6721 tgcacatgcc ggcacgcgtg gtgaagtcgc tcgaggcgga agactggtcg cgcctgggtg
     6781 ccccggtgtt cgtgcgtggg caagtccgca gttactcccg cctgctggga ttgaccacgg
     6841 caccgatgat ggccgcgttg gacgtcggcc ccgttgaacc gtccacgctg gtcagccgca
     6901 cgcacacgcc gaaggcgcag tggtgggcgg agcagatcgg gcgccggctg gtgtacatca
     6961 tcctgaccct gtcactggcg gtgccggcct gggtggcgac ccgccagcac ctgtcgggtg
     7021 ccagcgcagg cacgaccccg ctggacgccc cgaccgatcc gtctgcacgc ttgcctggcg
     7081 cgtccgagcc cgccagccct cgcaccgtgg tcgcctcgat ggcgccggtg gccacgcccg
     7141 ccatgcaagc cgctccggtg ggcgacatcg tgctgcgcac cagcgcagaa agctggctgg
     7201 acgtggtcgc accagacggt gcgtccatcg agaaaggcct cctgcctgca ggcagcgagc
     7261 gccgttatgc cgcgactcag gtcgcacgcc tgaccatcgg caacgcgtcc gcggcgcagc
     7321 ttgaacaccg cggccgcacc gtggacctgg caccgttcgc ccgcgcgaac gttgcacgct
     7381 ttgcggtatc ctccgacggt tcgctcgtgg cagccgattg accgtccggt cgatcctcgc
     7441 gggcactccc caatcagtga aaatgtcccg cccgagcatg gcgggcgaga gaaagcatgg
     7501 cgatcgacga cctgctcaac gagcatgaac agagtgagcg tgtccgcggc tggatccgca
     7561 gcaatgccat cggcctgatc ggcggcgtcg gcctcggcct gggcgccatc ttcggctggc
     7621 agtggtggca ggcccagcgc acgcaggcgc agatgggcgt gaatgcgcgc tacgcgcaag
     7681 cgatcgacgc ctatgaggcg ggcacgctgc ctgcggacaa aggccgcgac acgattgccg
     7741 cccttggcaa gggcaacccg acgcttggca cgctcgcggc cctgcaactg gccaaggtgc
     7801 aggccgaggc gggcaagcgc gacgacgcca tcgccaccct gcgcaacctg cgcgatgtcg
     7861 acgccgacct caggccgatc gtgcgccagc gcctggcgcg gttgctgctc gatgccagca
     7921 agcccaagga ggcgttgccg ttgctcgatg acgacaagga tccctccatg cttgaggtgc
     7981 gcggcgacgc ccggttcgcg ctgggcgaac gcgcgctcgc ccagaaggac tacctgaagg
     8041 cgctcgcgct ggtcgacgtg ggtgccccgg agcaccgcct gttgaccctg aagctgatcg
     8101 aagcgggcgg cacgccgccg cataccgagg acaacacctg atgatccacg cgtcgcatcg
     8161 ttccctgcgc atcgtcgccg ccactgcgct tgcggtggcc gtgctctcgg gttgctccac
     8221 catcaagaac gtcttcgacg gccgcggcaa ggacaagtcc accgagccga cggcgctggt
     8281 gcagatcagc ccgagcgtca gcgtcagcaa gctgtggagc gcctcgctgg gcaagggcga
     8341 agacaagctc gggattggcc agcatcccac gatcgtcgac ggacgcgtgt atgcggcggc
     8401 gatcgaaggc ggcgtgtccg ccttcgacct gcgcaccggc caggcgctgt ggcgctacgc
     8461 gtcggaactg ccgttgagcg gcggtccggg ggcaggcgaa ggcctggtcg tggtgggtag
     8521 cctggaggga gacgtgatcg cgctggacgc cgcgacgggt gccgagaagt ggaagtcgaa
     8581 ggtctccaac gaagtcatcg ccgcgcccgc ggtgggcggc gggctggtgt tcgtgcattc
     8641 caatgatggc cgggtgactg cgctcgatgc tgccaccggt gaacgccggt ggttctacag
     8701 cgccgatatc ccggcgctga ccgtgcgcgg caccggtggc atgaccgtgg ggccgggcat
     8761 cctcttcgtc ggcaacgaca acggcacgct ggtggcgctg tcgaccaccg atggctcccc
     8821 ggtctggcgc acgccggtgg ccgaaccgga aggccgcagc gagctggacc gcatggccga
     8881 cgtggacggc gaggccgtgc tggagaacac caccctgtac gccaccagcc acaagaacca
     8941 caccgtggcg atcgacggcc ccagcggcca ggtcatgtgg gatcgcggca atggcggtgc
     9001 gcgcggcttg ggcgtgagca attcggcggt cgtcgtcacc gatccgaccg gcaacgtctt
     9061 cggcctggac aagaacaccg gcggcgcgct ctggcagcag tcgggcctgg cctaccgcaa
     9121 tgtcagcgcg cctgcgatcc agggcgactt cgccgtggtc ggcgacctgg agggcgtgct
     9181 ccactggctg cgcctgaacg atggcgcctt cgccgcgcgc tccagcctgg gcggcgcgat
     9241 caccggcaag ccggtggtgt ccgacggcat cctggtggtg cagaccaccg agggccagct
     9301 cgccgcattc gcgctgcaat aagcggcctg gcctgccttc gggctggcat gctcccgcgg
     9361 ccccggctac cctgccgggg ccgtcgtgtt tttcgaacga ccggttttac gtgaccgcga
     9421 tccgcgccct gcgcaaaggt tgatccatgc ttcccctcgt cgccctggtc gggcgcccca
     9481 atgtcggcaa gtccacgctg ttcaatgcgt tgacgcgcac ccgcgacgcg ctggtgcacg
     9541 acgaaccggg cgtgacccgc gaccgcaact acggcgtttg ccgcctggtc gaaggtcgcc
     9601 cgttcgtact ggtcgatacc ggcggcatcg ccggcgagga cgaaggcctg gccggtgcga
     9661 ctgcgcgcca gtcgcgtgcc gcggcggaag aggccgacct gatcctgttc atcgtcgacg
     9721 gccgcgaagg cgcctcggca ttggacgacg acatcctgcg ctggctgcgc aagctgtcca
     9781 agcccacctt gctgatcgtc aacaaggcgg atggcatcga cctgcagtcg gcgctctctg
     9841 aattcgcccg ctacggactg ggcgatgcgt tgccgacgtc gtcggcgcac cgcaccggca
     9901 tcgacgaggt gctggccagg gtgatggagc gcctcccgca ggaaggcagc acggagaccc
     9961 tggacaccga cccgacccgc gtgcgcatcg ccttcgtcgg ccgtcccaac gtgggcaagt
    10021 cgacgctggt gaaccgcatc ctgggcgagg aacggatgat cgcctccgaa gtcccgggca
    10081 ccacccgcga ctcgatcgcg gtggacctgg agcgcgaggg ccgcaaatac cggctggtcg
    10141 acaccgccgg cctgcgtcgc cgggccaagg tcgaggaagc ggtcgagaag ttctcggtat
    10201 tcaagaccct gcaggcgatc gagcagtgcc aggtcgcggt gctgatgctg gatgccagcg
    10261 aaggcgtcac cgaccaggac gcgaccgtgc tcggtgcggt gctcgacgca ggcaaggcgc
    10321 tggtgatcgc catcaacaag tgggatggcc gcaccgacta cgagcgccag caggccgaga
    10381 cgctggtggc gcgcaagctg agcttcgtgg aatgggccga gaacgtgcgc atctcggcgc
    10441 tgcacggctc cggcctgcgc gaactgttcc gcgcgatcca ccgtgcgcat gcctccgcga
    10501 ccagggtgtt cagcaccgcc gacgtcaccc gcgcgatcga agccgcctac gaggccaacc
    10561 cgccgccggt ggtgcgcggg cacgtgccga agatgcggtt cgcccatccg ggcgcggaaa
    10621 gcccgccgac gttcatcgtc cacggcaccc gcctgaagtc gttgtctaac acctaccagc
    10681 gctacctcga gaacttcttc cgcaagcgct tcaagctggt cggcacgccg atccgcttcc
    10741 tgttccggga aggcgagaac ccgtacaagg acaagaagaa cgtcctgacc gatcgccagg
    10801 tcgccaagaa gcaacgcctg atccgccgcg tgaagcgcgc caagtaaccc cgatgatccc
    10861 gcgcggcgac ctcacgctgg gtatcctggc cggcggtcgg gcaagccgcc ttggcgggcg
    10921 cgacaaggcc tggctggagc gcggcggcac ggcccaggtg acgcgcatcg cgcgacgttt
    10981 cgatggcgaa tgcgccacgg tcctggtcag cgcgaatcgc gacctgccgc gttacgccgc
    11041 gcatggcctc gccaccgtcg tggatcggat ccccggcatc ggcccgctgg gcggcctcga
    11101 ggcactggca tcggcctgcg cgaccccatg gctgctgacg gttccggtgg atgtcgtgga
    11161 caccaacgac tgcctgttgc gcacgctcgc aagcgcaggc ggcgaggggg ccgttgccga
    11221 ggacgacgac ggcctgcagc cgctggttgc gctgtatcgc cttgcgcccc tgcgcgacgc
    11281 ggtggatgcc gcgatcacgg cgaacgcgct gtcgatccag gcgatgcagg caaggatccg
    11341 gttgccccgc gtgcgttttt ccggcttccg cttcggcaac ctcaacaccc cggaggacct
    11401 gcgcctggca ggctatgcgg atgactgact tccctgtttc gatcctgttc gacgaagccc
    11461 gcgacatcct gcgccgggtc gccgccgcgc atcgcctgcc ggtcgaacag gtggccctgg
    11521 cgcgcgccca cggccgcgtg ctggcgcacg acatcgtggc gccgatcgcg ctgcagccgt
    11581 tcgacaacag cgcgatggat ggctatgcct gccgccacgc cgacttgcgc gaggacgatg
    11641 cggtgcgact caccctggtg ggcgagcaat tcgccggccg cgcgctgggc ctgcaggtcg
    11701 gcgcgggcga gtgcgtccgc atcaccaccg gtgcgccgat gccggcgggc gcggataccg
    11761 tggtgatgcg cgaggacacg caggtcgatg cggatgcagt gaccatgcct gccggcattc
    11821 gccctggcgc gaacgtgcgc aaggccggcg aggacgcgag gcccggcgac ccggtgctgc
    11881 gggcggggac ccggctcaat gccgtgcacg tctcgttggc ggcgtcgctg ggcctggagc
    11941 ggctgccggt ctcggccagg cccacggtcg cggtgttcac caccggcgac gaactggtcg
    12001 aacccggcct gccgctgcaa cccggcgaga tctacaacag caatcgcgaa ttgctgatgg
    12061 gcctgctgcg cgcggaaggg ctggaacccg tcgcctggcc gacgcttccg gacgatcctc
    12121 tgcggatggc gtcgatgctc caggacgcag cttcgagctt cgatgtggtg atcacttgcg
    12181 gtgcggtgtc cgcaggggag aaggaccaca ttccggccat gttgcggata cacggcgccg
    12241 tgcatttctg gaaggtgcgg atgaagccgg ggatgccgct gctggccggc cgattggcga
    12301 acgcgcagtt cctgggcttg ccgggcaatc cggtctcggt gctggcgacc tttctcactc
    12361 tgggtcgcgt gctgatcgac ggcatgcagg gcagggcagg tcaaccacct cgcttcgcac
    12421 ggttgcaggt cggaatggaa aaagcgcatc cacgccgcga gttcgtgcgc gggacgctgc
    12481 aggtcgacga gacggggacg caatgggtca tccccaacgc ggcaaccggc tcgcaccggt
    12541 tgcgcgcgtc ggcggacgcg gacgccttgc tggtgctggc ggaaggcgcg caatcgctgg
    12601 ccaggggcga tgtcgtcgaa gtgctcacct attgatcgac gataatggcc gcatgcagat
    12661 ccgcatcctg agtccgaacg aggcacacgc gcgccagcgt gccggcgccg tgctggtgga
    12721 cgtgcgcgcg gcgcatgaac gcgcgttggg gatggccgtc gatgcgtccg gcatcgtccg
    12781 cgaagcgttg caggccgacc ccacggcgca tctcccggat cccggcatgg agatcctgct
    12841 gatctgccag cgcggcgctc gctcgctgca ggtcgcagag atcctcgccg cgcgcggcta
    12901 tcgcaacctg tcctccgtcg aaggcggcac cgatgcctgg gacgccgccg gtctgccgat
    12961 gcagcgcgcc gacgtggatg ccgatttcag cgagcgctat tcgcggcacc tgcgcctgcc
    13021 ggatgtcggc ctgcaaggcc agcaggcgct cgaggccgcg cgcgtgctgc tggtcggcgc
    13081 gggtgggctg ggctcgccgg cggcgtatta cctggccgcg gccggcatcg gcacgctgcg
    13141 catcgccgat gacgacgtgg tcgatcgcag caacctgcaa cggcagatcc tgcacaccga
    13201 cgcacgcatc ggcatgccga aggtcgaatc cgccacgctt gcactgtcgg cattgaaccc
    13261 gcgcacgcgc atcgaggccg tgcgcgaacg catcacttcg gacaacgtcg aacgcctgct
    13321 cgatggcatc gacgtggtgg tggatggcgc cgacaatttc cccgcgcgct acctgctcaa
    13381 cgatgcctgc gtgaagctcg gcaagccgct ggtgtatggc gcggtgcatc gcttcgaggg
    13441 ccaggccagc gtgttcgatg ccggtcgcca tcgcggaagt gcgccttgct atcgatgcct
    13501 gttcccggaa ccaccgccgc ccgagtccgc gccgaactgc gccgaagcgg gcgtgctcgg
    13561 cgtgctgccg ggcgtgatcg gcctgctgca ggccaccgag gcgatcaagt tgctgctggg
    13621 catcggcgac ccgttgcgcg ggcgcctgct gcagttcgat gcgctggcga tgcggttccg
    13681 cgaaacccgg ttgctgcccg acccgcaatg tccggtctgc gcgcccgggt gcgctttccc
    13741 tggttatgtc gattacgcgg cgttctgcgc cggaggcgag gcttgatggt ggtggagacg
    13801 aagcgcgcag gcgggttgtc gcgccggtgg cggtggctgg cagcggtggc gctgttgttg
    13861 tcctcggcat gggcgatggc cgccgaccaa tgccccgcat tgcgcgatcg acagggcgat
    13921 accgaggtcg cgacccgcat cgccgcgatc gcctgcgacg aacacctgcg ctggaaccgc
    13981 cccttcatcg atgcggacgg ccggctggcc agcctcgcgg tgtacgaggc cgaaagccgc
    14041 ggcctgcagg atggcggctc gccgtggcgc cgggtcgcgt tctactggca gtccaccggc
    14101 ctgctgtcgc cgatggccgc caagtcgggc gccaccgact gcaactacgc gatcaacagc
    14161 agctaccccg gcctgggctg ccgcggcttc atcgtcgaca acccgtggtc ggcggccttc
    14221 atcacctggg tgatgcagcg cgccggcgtg cccggcttcg tcagttcggc cagccatttc
    14281 gactatgtcc gcgcggcgcg caccaatccc gcgcgcagtc cgtacgtgtt cctggatccg
    14341 atggccgccg cccccgcggt cggtgacttg ctgtgctacg tgcgcaccaa tcgcgtgtat
    14401 ggccatcgcg ggctcgccag cgccatcgac ggcggcacta cgggactcgc catgcattgc
    14461 gatgcggtgg tcgcggtcaa tcccggcggc gacggcaagg cctatgccat cggcggcaat
    14521 gtgcagcagg cggtcacgat gcgccagttc aacctcaatg ccagcgggca gttgtggggc
    14581 gtgccgcagc gcaccgaggg cgatgtcgag tgttcgccgg acacgcagtc cgcgtgcaat
    14641 ttcaaccgcc aggactgggc ggtgctgctc aagctcaagt cgcaggcgga actggccctg
    14701 atcggcccgg tgcagccgcc gacctacatg cccaaccagg tggtgccgca ggcatgttgc
    14761 gtgaattgcg tggtgggttc gggcgtgccg cgttgcccgg cacccggcca gccgcccctg
    14821 cagccgcagc cgcaagggca gctgcagggc tctgaatgac cctcaggggg tggtcgccgc
    14881 cggcttggcg cgcgaccgcg gcttggcgcg gtggtcgacc tgcacgccca ggacgacgtg
    14941 gcagtggatg cagctgtagc tgaggcagcg cgcgttgctg gtgccatccg ccacgccgac
    15001 gacgttctcg atattgacgt gcaagacgcg cttgccgcac ttgggacatt cgccgtggat
    15061 catcgccatg tggccccctg tagggtctcg ctgccggccg acggcggtct caggaagagc
    15121 gcgtgcgcgc ctcgaaggcg gccagctgct ccggcgtggc ttcggcctgg tgccgcgcct
    15181 tgaagtcagc gaagggcata ccgtacaccg cttcccgcgc ctcgtcgcgg gtcatcggct
    15241 ggccgctcgc ctcggccgcc tcgcggtacc agtcggccag gcaattccgg cagaacccgg
    15301 ccaggatcat caggtcgatg ttctgcacgt cggggcgctc gaccatcagg tggtcgcgca
    15361 ggcggcggaa cgtggcggct tcgagtgcgg tgttgtcgac gggtgcggtc atcggcgtgc
    15421 ctgggtgggc gataatgcgg gcccgactgt agcgcaacgc caccgcatgc ccgccaagat
    15481 cctcgacgga aagcgcatcg ccgacgacct cctgctcacc ctcaaggcgc aggtcgacgc
    15541 gcgcgtggcc gccggcaagt cgcggcccgg gctggcggtg gtgctggtcg gccaggatcc
    15601 ggccagccag tcctacgtgc gcaacaagcg ccgggcggcg gagaaagtgg gcatcgaggc
    15661 gctggacttc gacctgccgg cgggcaccac cgaagccgag ctcctcgcgc tggtcgatcg
    15721 cctcaatgcc gaccccaaag tgcacggcat cctgatccag ttgccgttgc ccggcatccg
    15781 cgacgccacc gcgttgatcg aacgcatcca tcccgacaag gacgtggacg gcttccatcc
    15841 ggagaacgtc ggccacctgg ccctgcgcca gttcggcctg cgtccatgca ccccgcgcgg
    15901 catcaccacg ctgctggcct acaccgacaa gccggtgcgt gggcagagcg cgaccatcgt
    15961 cggcgtgtcc aaccacgtcg gccggccgat ggcactggag ctgctgatcg caggctgcac
    16021 ggtcaccagc tgccacaagt tcaccccgct ggacgtgctg aagcgccacg tcggcgaagc
    16081 cgacatcctg gtggtcgcgg tcggccgccc cgggctgatc ccgggcgact ggatcaagcc
    16141 gggcgcggtg gtgatcgatg tgggcatcaa ccgcctggac gatgggcgcc tggtaggcga
    16201 cgtcggtttc gccgaggccg cgacccgcgc cagctggatc accccggtgc ccggcggcgt
    16261 ggggccgatg accgtggcca cgctcatgca gaacaccctg gaagcggcgg agctggctga
    16321 ttcgcgctga agttcacctc tccccttgtg ggagaggtcg aagcggcaag gccgcttcgg
    16381 gagagggggc ttttgccgat aggagcaacc ccctcccagc ctccccctgc ttcgcaaggg
    16441 gaggagacaa gcgcttcgca ggggaaggag caaaccggct agaatggcgc gcttcacaag
    16501 ccaccggacc ccgcatgctc cgcatccagg ctgaagcgct cacctacgac gacgtctcgc
    16561 tcgtccccgc gcactcgacg atcctgccca aggacgtcac cctgaccacc cgcctgacca
    16621 aaggcctctc gctgcgcttg ccgatcgtgt ccgcggccat ggacaccgtc agcgaatcgc
    16681 gcctcgcgat cgccctggcc caactgggcg gcatcagcat catccacaag aacatgagcg
    16741 ccgaggcgca ggctgcgcag gtcgcgcggg tcaagaattt cgaggcgggc gtgatccgcg
    16801 atccgttcac cgtaggcccc gacgccacca tcggcgaagt cctgcgcctg acccgcgccc
    16861 gcaacatctc cggcgtgccg gtcgtggacg agtcgggaca actggttggc atcgtcaccg
    16921 gccgcgacat gcgcttcgag aagaagctgg acgatccggt ccaccacatc atgaccaaga
    16981 aggatcgcct ggtcacggtc aaggaaggcg cgtcggacga cgaagtgatc ggcctgctgc
    17041 acaagcaccg catcgagaag gtgctggtgg tgagcgacga cttcgccctg cgcggcctga
    17101 tcacggtcaa ggacatccag aagaagtcgg acaacccgaa cgccgcctac gacagccgcg
    17161 aacgcctgct ggtcggcgcc gcggtcggcg tgggcggcga taccgagcag cgcatcgagg
    17221 ccctggtcgc cgccggcgtg gacgtcgtga tcgtggatac cgcgcatggc cattcgcagg
    17281 gcgtgatcga ccgcgtggcg tgggcgaaga agcgcttccc gtcgttgcag gtggtcggag
    17341 gcaacatcgt caccggcgat gccgcgctgg ccttgatgga tgccggcgcg gacgcggtga
    17401 aggtcggcgt gggcccgggt tcgatctgca cgacgcgaat cgtggccggc gttggcgtgc
    17461 cgcaggtcac cgcggtgtcg atggtcgccg aggcgctgca ggaccgcatc ccgctgatcg
    17521 ccgatggcgg catccgctat tccggcgaca tcggcaaggc gatcgtcgcc ggcgcgtcca
    17581 gcgtgatgat cggtggcctg ttcgctggca ccgaggaaag cccgggcgag accgaactgt
    17641 tccagggccg cagctacaag agctaccgcg gcatgggttc gctgggcgcg atggagaagg
    17701 gcagcaagga tcgctatttc caggacgcaa gcgacgccga caagctggtg ccggaaggca
    17761 tcgagggtcg cgtgccgtat cgcggcccgc tgagcggcgt ggtccatcaa ctggtcggcg
    17821 ggctgcgcgc cacgatgggg tatgtcggtt gcgccaccat cgaggagatg cgcaccaagc
    17881 cgtccttcgt gcaggtcacc ggcgccggcc agcgcgagag ccacgtgcac gacgtgcaga
    17941 tcaccaagga accgccgaac taccgcgcgt cctgatgcca tccacgaagg ggtgaggcgg
    18001 tgcgttcgcg aaccactggt tcgcgcatcg ccgagcgccc ggccatggat ggccgggcag
    18061 gcgcatatcg aagccgggta gtggggccat ggacggcaac gaaaattttt gccgaagcca
    18121 acgcacggac ccaagatgat gaccaacatc cacaacgaca agatcctcat cctggatttc
    18181 ggcgcgcagt acacccagct gatcgcccgc cggatccgcg agatcggcgt gtactgcgag
    18241 atctgggcct gggatcacga tcccgcggag atcgccgcgt tcggcgcgaa gggcatcatc
    18301 ctctctggcg ggccggaatc gaccaccgaa gcgggcagcc cgcgcgcacc gcagcaggtc
    18361 ttcgagtcgg gcgtgccctt gctcggcatc tgctacggca tgcagaccat ggccatgcag
    18421 cagggcggca tggtcgaggg cggccaccac cgcgagttcg gctacgccga ggtcgaagtc
    18481 accaccccga gcgcgctgct cgacggattg caggacaagc cggggctgcc cgcgcggctc
    18541 gacgtctgga tgagccacgg cgaccgcgtc actgcgctgc cgagcggatt caacgtgatc
    18601 gcgcgcaccg ccggcgtgcc gatcatcgcg atggccgacg aggcccgtcg ctggtacggc
    18661 gtgcagttcc atcccgaagt cacccacacc agttccggcg aactgatgtt gcgccgcttc
    18721 gtggtggaca tctgcggctg ccgcacactg tggactgccg ccaacatcat cgaagaccag
    18781 atcgcccgcg tgcgcgcgca ggtcggcgac gacgaggtcg tcctggggct gtccggtggc
    18841 gtcgattcgt ctgtcgttgc cgcgctgctg catcgcgcga tcggcgacag gctgacctgc
    18901 gtgttcgtcg acaccggcct gctgcgctgg aacgaaggcg accaggtgat ggcgatgttc
    18961 gcccagaaca tgggcgtcaa ggtcctgcgc gtgaacgccg ccgaccgata tttcgacagg
    19021 ctgcggggca tcgaggatcc ggaagcgaag cgcaaggtca tcggcaacct gttcgtcgag
    19081 atcttcgacg aggaatcgca gaagctcgcc aacgcgaagt ggctggcgca gggcacgatc
    19141 tacccggacg tgatcgagtc ggccggcagc aagaccggca aggcgcacgt gatcaagagc
    19201 caccacaatg tcggcggcct gccggaacac atgaagctcg gcctggtcga gccgctgcgg
    19261 gaactgttca aggacgaggt gcgccggctc gggattgccc tcggcctgcc gcgcgacatg
    19321 gtctaccggc atccgttccc gggccccggc ctgggcgtgc gcatcctggg cgaagtgaag
    19381 cgcgaatacg ccgaactgct ggcgcaggcc gaccacatct tcatcgacga actgcgcaag
    19441 gccggcctgt acgaccgcac cagccaggcg ttcgcggtgt tcctgccggt gaagtcggtt
    19501 ggcgtggtcg gcgacgcccg cgcctacgaa tgggtgatcg cgttgcgcgc ggtcgagacc
    19561 atcgatttca tgaccgcgca ctgggcgcac ctgccgtacg agttcctggg gaccgtgtcc
    19621 aaccggatca tcaacgaact gcg
//
LOCUS       DAOOCX010000040        19117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2543_length_19117_cov_8.666142, whole genome shotgun sequence.
ACCESSION   DAOOCX010000040 DAOOCX010000000
VERSION     DAOOCX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 19117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19117
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2543_length_19117_cov_8.666142"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PK399_05595"
     CDS             complement(<1..398)
                     /locus_tag="PK399_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005915394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPW12626.1"
                     /translation="MAPKKTSYPKQDIKVLLLEGISPNAVETFRAAGYSNIVTHAKSL
                     PEDELRQAIADAHFIGIRSRTQLTAGVLADARRLLAVGCFCIGTNQVDLEAAELAGVP
                     VFNAPYSNTRSVAELVLAEAIMLLRGIPQKS"
     gene            611..1993
                     /locus_tag="PK399_05600"
     CDS             611..1993
                     /locus_tag="PK399_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPW12627.1"
                     /translation="MTDPRIADLQASVPGLRLKTDPADLEHYGRDWTRRWTPAPLAIA
                     LAGNVEEVQSIVRWAKANAVAIVPSGGRTGLSGGAVAANGELVVSLERMNQVLGFDAI
                     DRTLTVQAGIPLQLAQEAARSHGLQYPVDFAARGSCSIGGNIATNAGGIRVVRYGNTR
                     EWIAGLKLVTGDGELLDVGRSLVKNSSGYDLRHLAIASEGTLGIVVEATLKLTDPPPP
                     TNVMLLALPSFDALMQVFDAFRARLPLQAFEFLTDRALHHVLAHGAQAPFAETHPYYV
                     VTEFASDEGSETEALAAFEQCMEAGLVSDGVISQSDAQAAQLWRLREGITESLAKHVP
                     YKNDISVRLSAMPAFLAETQALLGEAYPHFDVVWFGHIGDGNLHINVLKPDDMAQTDF
                     VADCDRVTKLLAERLHAHGGSISAEHGIGLVKKPYLSSTRSATEIATMRGIKAVLDPA
                     GIMNPGKLFD"
     gene            complement(1994..2581)
                     /locus_tag="PK399_05605"
     CDS             complement(1994..2581)
                     /locus_tag="PK399_05605"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPW12628.1"
                     /translation="MNSTLASVTLAGHGIRLAPLAREHIAPLQDAVDDGDIGALNYVN
                     VPGREGIPAWVEHALAMRDAGRELPFAIVSDERVVGSTRFYDIDMSVPTLAIGYTFHA
                     ASVWRSHVNTANKRLMLGHAFDTLGAQSVFFHTSHLNLRSQAAIERLGAQRDGILRAH
                     KRHKDGSLRNTYTYSITAPEWPAIRERLNARLAAR"
     gene            complement(2713..3279)
                     /gene="yeiP"
                     /locus_tag="PK399_05610"
     CDS             complement(2713..3279)
                     /gene="yeiP"
                     /locus_tag="PK399_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009603592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HPW12629.1"
                     /translation="MKAYDIKKGNVVEHNGSVYQIRDIERSSPQGRGGNVKFRFTMYS
                     VPGGTKLDISVGGDDELKDVELSRRQVTYSYKDGDAFVFLDDEDYTPYTLDAEVVGDA
                     AGYIVDDLTGCYVQIIDDLPVAIQLPASVAIEVVETPPELKGGTATKRPKPARLSTGI
                     EIMVPEYIGNGEKVWVNTATGEFAGRAD"
     gene            complement(3290..3580)
                     /locus_tag="PK399_05615"
     CDS             complement(3290..3580)
                     /locus_tag="PK399_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12630.1"
                     /translation="MTDYGSRAERWVAQKWPAIRARGMWRFVLLKGVAFWGGLMFAFL
                     AIMMLVQFGAAHPRFPLLLAVALPLCAIGGLCWGLLTWYFNERIYRALQTSR"
     gene            complement(3577..4347)
                     /locus_tag="PK399_05620"
     CDS             complement(3577..4347)
                     /locus_tag="PK399_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW12631.1"
                     /translation="MQLSNARAVITGGVSGLGFAVARHLVAHGGKVALFDVNDDKGAA
                     AVAELGEANARYFRTDVTDEAGVIANVDAARDFLGGLNVAINCAGILGAGRVLGRDGA
                     MKLSQFQATVMVNLVGSFNVAKACAERMQGNEAGVDGERGVIVNTASVAAYEGQIGQA
                     AYSASKGGVVGMTLPMARELARFGIRVMTVAPGIFWTPMVDGMPDDVQQSLAASIPFP
                     SRLGKPEEFADLVGYILGNTYLNGETIRLDGATRLAPK"
     gene            complement(4597..5100)
                     /locus_tag="PK399_05625"
     CDS             complement(4597..5100)
                     /locus_tag="PK399_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW12632.1"
                     /translation="MALHAGTSIRPITADDDAAMAAIIRTVMPEFGATGCGFAISDPE
                     VDWMSRAYAEPRHAYFVVEREGRILGGAGVAPLAGDSGETCELRKMYFLPEARGIGAG
                     AAMMARCLQAARDAGFHQCYLETLTGMDAAMRLYERSGFRRIDGPMGATGHGGCNTFY
                     LLDLASG"
     gene            complement(5103..7628)
                     /locus_tag="PK399_05630"
     CDS             complement(5103..7628)
                     /locus_tag="PK399_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW12633.1"
                     /translation="MASTSTDALAQALRAAAAGPGISPALHALLLRAGDALDGAQAAI
                     EAGNLRYRALFDAVPDPVSVLARDGTVLDLNKAGMRAYNRPREDIIGQPIEVLNPDLP
                     RDHLVPVLETLDRGETYVIEVTNMRADGTRFPVEVHSANLLYDGQDAMVAVARDLSAR
                     HDAEVRYNSLIEGIDKAITLQDRQGRVHYMNAAASRIYGIGGDESIHVEPDWNDWLVV
                     DEDGRFLPFEAHPASRALATGQAVSSQVLGLFRRSTQRLLWLSVTAVPQFAPGADQAD
                     MVLTLSSDITELQRDKALFDRVQELAQIGGWQWDRASGALYLTREASRILGAAQALDA
                     MEQVLACLREDDRERLRRTLESICDGTGFELELQGAQADGSPFWVRMIGEPDPHDPGS
                     DRLSGTLQDITERKHAEHALRLQAQTDALTGLMNRDAILEQITLRMRNPGRSGLCLLY
                     IDLDRFKIVNDVLGHNAGDTLLVDAAQRIAGAVGSEGLIARFGGDEFLVACRADDDHE
                     RPQRLAKQIQQAFGEPFRLGKDEFNVTTSIGIARAPDDGETPNQLIQSADVAMYDSKR
                     RQRNGFQSFSPELASRQHERLQTETQLRRALDKGEFHLVYQPQVDLRTGRILYAEALV
                     RWRNPQLGEMRPDRFIELAETTGDIIRIGQWVLQEACAQMRRWREQDLPIQRVAVNVS
                     YRQFLAEDFGQGVQRVLQDAGLPGHALELELTERVLVDDAADSLEVFDRLRRLGVQLS
                     IDDFGEGYSALNYLRRLPIHALKLSQTFIKGVPGKPSDVAICQAVAGIASSLGLGLVA
                     EGIETESQRDFLIRLGVKVGQGFLFAPGLLPGEFAKRVIAQQD"
     gene            complement(7667..8566)
                     /locus_tag="PK399_05635"
     CDS             complement(7667..8566)
                     /locus_tag="PK399_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPW12634.1"
                     /translation="MTFPAEVRIVEVGPRDGLQNESVDIGTTAKIELIDRLSRTGLRS
                     IEATAFVSPKWVPQMADAAEVLAGITRAPGISYPVLVPNEQGYERARAAGAAEVAVFT
                     AASEAFNLRNTNATVAQSLERFRPVLQRANADGVRVRGYVSTVLGCPYQGEVPLADVV
                     RVARDLHAMGCYEVSLGDTIGVGTPAKARAMLKAVAAEVPMAALAIHFHDTYGQALAN
                     VLACLEEGVAVVDSAVSGAGGCPYAKGASGNVASEDVVYMLHGLGIRTGIDLPLLAET
                     GRWLAGLLGRDTGSKVGKALGAG"
     gene            complement(8568..9203)
                     /locus_tag="PK399_05640"
     CDS             complement(8568..9203)
                     /locus_tag="PK399_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12635.1"
                     /translation="MQRTARLALLLLSLCLGGCSTTVFESLPTGTTTDCDPAWPGRWE
                     PVGTADDPMKPQGVVEIAPDCRSATTKGETKPIRLTLIDTRAGQYLQLHNDSGKPDCV
                     GPGNTRCGAALLRYERDGDTMRLYDADHAWIAAAIKAKKIDGYSMRPDTKGLKTKEPT
                     YNNFIAGNPDRIAKLLRKYPALFVREPLMVLRRVPVDAASAQPTPPATDGN"
     gene            complement(9206..11200)
                     /locus_tag="PK399_05645"
     CDS             complement(9206..11200)
                     /locus_tag="PK399_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HPW12636.1"
                     /translation="MFDTILIANRGEIACRVIRTARRLGIRTVAVFSDADADAQHVRL
                     ADEAHHIGGARPQDSYLRGDAIIEVAKQTGAQAIHPGYGFLSENADFADAVLAAGIAF
                     IGPSGASMRKMGSKAGAKDLMAAAGVPVVPGYTGEDQSPDLLQREADKIGYPLMIKAA
                     HGGGGKGMRIVRTRDEFLANLESCQREARNAFGRDRVLLERYIEHPRHIEIQVFGDSH
                     GGAIHLNERECSAQRRYQKVLEESPSVFLTPELRARMGAAAVQAAHAIDYANAGTVEF
                     IVGADGGFYFMEINTRLQVEHPVTEEVTGLDLVEWQLRIAAGEQLPLAQEQIRQHGHA
                     IEVRLYAEDPDAGFLPGSGTLQTLRLPAASRHVRIDSGVVEGDTVTIFYDPMIAKLIV
                     WDEDRPRALARLREALAACDITGPKSNIAFLERLVRHPAVVEGSIDTGYLDRHLDEFL
                     SPAQTDRTLLIAAATTRLLAQEAQARAAAAGSHDAGSPWAIADGWRLGHGGSRRLAFV
                     DGAARIELVARGHGGDYTIEVDGGSHAVSGARFDGDALSARIDGGMRRFHAMQEGDDV
                     LVHDGRQRLRLHAEAVYRRAAGDAGSAEHRIRAPMPGRVVVVQKQPGDPVAAGDVVLV
                     IEAMKMELALKAPRDGVMAELRAQAGDFVDGDAVLATLEQ"
     gene            complement(11200..11985)
                     /locus_tag="PK399_05650"
     CDS             complement(11200..11985)
                     /locus_tag="PK399_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPW12637.1"
                     /translation="MSDPLQLHRDGPVARLRMTRAEVHNAFDAALIAGLTAALDEVAA
                     DAAIRVLVLEAEGASFSAGADLHWMRGMAAASETENRTDSLALARLMRTLDELPKPTI
                     AAVQGSAFGGGVGLVACCDIAIAARGAKFGLTESRLGLLPAVISPYVIAAIGARNARR
                     YFATAEIFDTDEALRIGLVHDVVNPTALDTAVQRQIDLLLKAGPIASANAKKLVRDVC
                     AHADGARHDADNASLIARLRVSPEGQEGLSAFLDKRKPHWVQD"
     gene            12111..12359
                     /locus_tag="PK399_05655"
     CDS             12111..12359
                     /locus_tag="PK399_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HPW12638.1"
                     /translation="MTLTELARRTPAIVDAVEDRTPNDPIARRLRELGFVAGEEVVVV
                     AAGPLGAEPLLVQVGFTRFALRRVEAARVRLRSNGAGA"
     gene            12356..14215
                     /locus_tag="PK399_05660"
     CDS             12356..14215
                     /locus_tag="PK399_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HPW12639.1"
                     /translation="MNAAVAAPRIALVGNPNCGKTALFNLLTGSRQKVANYAGVTVER
                     KEGRLHAPSGKHYAVLDLPGAYSLNAASLDEAVTRDLIRGFYPGEAAPDLLVCVVDAT
                     NLRLHLRFVLELRALGKPMLVALNMADAAKKRGIDIDVAALQRELGVPVVETVAVRRG
                     GADRLLATLDAVVAHPHAVADALPEGTDVHAETRRLLAATVRMPERTAKIDDALDRWL
                     LHPVFGLSTLAVVMFLIFQAVYAWATPVMDLIEAGTTALGAWAGTMLPAGPLNSLVVE
                     GIIAGLGGVIVFLPQILVLFAFILALEESGYLPRAAFLLDRMMAGAGLSGRSFIPLLS
                     SFACAIPGIMATRSIQDPRDRLATILVAPLMTCSARLPVYALLIGAFIPSREVWAGIN
                     LQGLVLFALYMAGIFSALAVSWVMKKWRRDKGEHPLMLELPSYRLPNPRDVLIGLWER
                     ALIFLRRVGGIIFALTVLLWVLLSFPAAPVDATMPAIDYSFAGRIGHAMTAVFAPLGF
                     NWQICIALIPGLAAREVAVSSLATVYALSAVDDEAAAQALTPLITNGWSLATGLSLLV
                     WFIYAPQCLATWAAIKRETGSWKTMAMAAGYLFALAYLASFATYHVAVALGGG"
     gene            14219..14452
                     /locus_tag="PK399_05665"
     CDS             14219..14452
                     /locus_tag="PK399_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12640.1"
                     /translation="MASGLYAQYAVIVLVVMASVLIVMRSQFPAATRKLRIAMALPLL
                     REGTPAWMKELGRRIAPKPRGGVNACGGCDNCD"
     gene            complement(14493..14849)
                     /locus_tag="PK399_05670"
     CDS             complement(14493..14849)
                     /locus_tag="PK399_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12641.1"
                     /translation="MSAVRPGTTGATRTCPHCKATILESSSVCPACRGHLKFDAPASD
                     ATQRFVPLKVEGTVSAPDDGAFEYSMVVVIRNERGEEIGRHVAGVGAVFPGESRSFEV
                     SVEAVTASPGKRRLRH"
     gene            complement(14846..15313)
                     /locus_tag="PK399_05675"
                     /pseudo
     CDS             complement(14846..15313)
                     /locus_tag="PK399_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007967362.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
     gene            complement(15492..17042)
                     /locus_tag="PK399_05680"
     CDS             complement(15492..17042)
                     /locus_tag="PK399_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPW12642.1"
                     /translation="MSPFNILGLDRKADLAQVKRAYAKLLRTHRPDEDPTGFQRVHEA
                     YQACLEQLRWREQGWEDEDEDDDGIAVEPDADDGVADPAAGNASVGMLLAPLDVATIP
                     REPGDPASDGIDLRVSDFFDAEAFAHDLGTRLRSETPQAVETWLQAHDDLYALDRKRA
                     LQASVVDMLETLDAQAAARHFDTVTRFFGMDTVSGTDSWLHHRLDAIQQRFGDAADFE
                     RVLRTHAGPDATWADQGIARELLQPFSWWRRLCLIACPGLPGRTGALLQALQAADPVS
                     ASTRLDPPSRRFWERATDRRALRWQRLSVMAVRLALWASAIGIAVSMLDEKTGFDAES
                     PTRWATLFAWPYGLWLAYAVVVLGLIRFRDYNHSRMQWDWVLLMTAGGLACGVVAFAA
                     GANGTLAFIMTTVLWIGARKDGERGSSASQGASLAAGATGYGLALLVLHQLVGDSIEI
                     RYLACIAAVYAFGSQAIHDVLLARKRGIALVRARTQTGALWRIFQVQGALLAVLLVLV
                     LMRGKTGA"
     gene            complement(17046..18743)
                     /locus_tag="PK399_05685"
     CDS             complement(17046..18743)
                     /locus_tag="PK399_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HPW12643.1"
                     /translation="MIVGIDLGTTHSLIGCFEGNAPRLFPNALGDLLTPSVVSIGEDG
                     GILVGQSARDRLITHPQASVAAFKRWMGSNRSTRLGKREFRPEELSALVLRSLLADAE
                     AALGEKVREAVISVPAYFGDAQRKATRAAGELAGIKVERLVNEPTAAALAYGLQERID
                     GSTFIVLDLGGGTFDVSILEIFDGVMQVHASAGDNHLGGEDFLDVMADACCRELGINR
                     RALPPNELALLLSRLEVAKKQLSSATEAVVNVASGGTAREWRIDDVGFQRLAEPLLQR
                     VRAPIERALRDAKLSPGDLSEVVMVGGASRMPVFTKLVSRMLGRLPLRHVHPDQAIAL
                     GASVAAGMKARDQALEEVVMTDVCPYTLGIATAREDEHGRVSSGHFSPIIERNATVPV
                     SRSEQFCAMHDGQREIKLEIFQGESPRVANNVLLGTLDIRLAPKMQKNQAAIEVRFTY
                     DINGVLQVESTTLGTGEKRELILQDNPGVLDEAEIRERLASLSALKMHPREDQANLAT
                     IARIERLYEERLDQRSLLQDWLARFTGVLESQDRARIERERGELSRALDQIEARGFD"
     gene            complement(18767..>19117)
                     /locus_tag="PK399_05690"
     CDS             complement(18767..>19117)
                     /locus_tag="PK399_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPW12644.1"
                     /translation="NYAMCGRAYGARFLWMWPNARISVMGGEQAASVLATVKRDGIEL
                     KGGRWSAEEEDAFKAPIREQYETQGNPYYASARLWDDGIIDPADTRRVLGLGLSASLN
                     APIEDRTRFGVFRM"
BASE COUNT         2837 a         6452 c         6638 g         3190 t
ORIGIN      
        1 gacttctgcg ggatcccgcg caacagcatg atcgcctccg ccaggaccag ctcggccacg
       61 ctgcgggtgt tggagtaggg cgcgttgaac accgggacgc cggccagttc cgcagcctcc
      121 aggtccacct ggttggtgcc gatgcagaag cagccgaccg ccagcaggcg cctggcatcg
      181 gccagcacgc cggcggtcag ctgggtgcgc gaccggatgc cgatgaagtg ggcgtcggcg
      241 atcgcctggc gcagctcgtc ctccggcagc gacttggcgt gggtgacgat attgctgtag
      301 ccggccgccc ggaacgtctc caccgcattg gggctgatgc cctcgagcag cagcaccttg
      361 atgtcctgct tggggtagga ggttttcttc ggggccatgg cacggcaaca ggcgagggga
      421 gccgcactat gccagatgca aggcggcttt tgccgcactg cgtcatgcca tttcggcgcg
      481 cgtccatgcc gtgtccacgc gccgggaaag ttgcggctga aaccgcactg ccatgcgcgc
      541 cgcgggcgct ggacgcatcg gtgcgcggct ggcacgctgc gggctccctc gcgtcgccat
      601 cgcccatccc atgaccgacc cccgcatcgc cgacctgcaa gcgtcggtgc ccggcctgcg
      661 cctgaagacc gaccccgccg acctcgagca ctacggccgc gactggaccc ggcgctggac
      721 gccggcaccg ctcgcgatcg ccttggcggg caatgtcgag gaggtgcagt cgatcgtgcg
      781 ctgggcgaag gcgaatgcgg tcgccatcgt cccgtccggc ggccgcacgg gattgtccgg
      841 tggcgctgtg gcggcgaacg gcgaactggt ggtcagcctg gagcgaatga accaggtgct
      901 cgggttcgat gcgatcgacc gcacgctgac ggtgcaggcc ggcatcccgc tgcaactggc
      961 gcaggaggcg gcacgcagcc atggcctgca gtacccggtg gatttcgccg cacgcggctc
     1021 ctgcagcatc ggcggcaaca tcgcgaccaa tgccggcggc atccgcgtgg tccgctacgg
     1081 aaacacccgc gaatggatcg ccgggctgaa gctggtcacc ggcgatggcg agctgctgga
     1141 cgtcggccgc agcctggtca agaactccag cggctacgac ctgcgccacc tagcgatcgc
     1201 gtccgagggc acgctgggca tcgtggtcga ggccacgctg aaactcaccg atccgccccc
     1261 accgaccaac gtgatgctgc tggcgctgcc gtcgttcgat gcgctgatgc aggtgttcga
     1321 tgcgttccgc gcgcggctgc cgctgcaggc attcgaattc ctcaccgacc gcgcgctgca
     1381 ccatgtgctg gcgcacggcg cgcaggcccc atttgcggaa acccatccgt attacgtggt
     1441 gaccgaattc gccagcgacg aaggcagcga aaccgaagcc ctcgctgcgt tcgagcagtg
     1501 catggaggcc ggcctggtca gcgacggcgt gatcagccag agcgacgcac aggccgcgca
     1561 gctgtggcgc ttgcgcgaag gcatcaccga aagcctggcg aagcatgtgc cgtacaagaa
     1621 cgacatatcg gtgcgcctgt ctgcgatgcc ggccttcctc gccgagacgc aggcgttgct
     1681 gggcgaggcc tatccgcact tcgacgtggt ctggttcggc cacatcggcg acggcaacct
     1741 gcacatcaac gtgctcaagc cggatgacat ggcgcagacg gacttcgtcg ccgattgcga
     1801 tcgcgtcacc aagttgctgg ccgaacgcct gcacgcgcat ggcggcagca tctccgccga
     1861 gcacggcatc ggcctggtca agaagcccta tctgtcgagc acgcgcagcg ccacggagat
     1921 cgcgacgatg cgcgggatca aggcggtgct ggatccggcc gggatcatga atccgggcaa
     1981 gttgttcgac tgatcagcgg gcggcaagac gcgcgttgag gcgctcgcgg atcgccggcc
     2041 attccggcgc ggtgatcgaa tacgtgtagg tgttgcgcag cgagccgtcc ttgtgtcgct
     2101 tgtgcgcgcg caggatgccg tcgcgttgcg cgcccagccg ttcgatcgct gcctgcgagc
     2161 gcaggttgag atggctggtg tggaagaaca ccgactgcgc gccaagcgta tcgaaggcgt
     2221 ggccgagcat cagccgcttg ttggcggtgt tgacgtggct acgccacacc gacgcggcgt
     2281 ggaaggtgta gccgatcgcc agcgtgggca cgctcatgtc gatgtcgtag aagcgcgtgc
     2341 tgccgaccac gcgctcatcg ctgacgatgg cgaacggcag ctcgcggccg gcgtcgcgca
     2401 tcgccagggc atgctcgacc cacgccggga tgccctcccg gcccggcacg ttgacgtagt
     2461 tgagcgcacc gatgtcgcca tcgtccaccg cgtcctgcag cggcgcgatg tgctcccgcg
     2521 ccagcggcgc caggcggatg ccgtggccgg cgagggtgac ggaggcaagg gtggaattca
     2581 tgtgctttgg ctcgtcattc ccgcaacggc gggcatcgtt tcgcagccgc acggctgtgt
     2641 atccagcgtc tgacttgttt ggatgcaacc gctggatccc cgccttcgcg gggatgacag
     2701 cagcggcctt gctcagtccg cgcgtccggc gaactcgccg gtcgccgtgt tgacccacac
     2761 cttctcaccg ttgccgatgt actccggcac catgatctcg atgccggtgc tcaggcgcgc
     2821 gggcttcggg cgcttggtcg cggtgccgcc cttgagttcc ggcggcgtct cgaccacttc
     2881 gatcgccacg ctggccggca actggatggc caccggcagg tcgtcgatga tctgcacgta
     2941 gcagccggtc aggtcgtcga cgatgtagcc ggcggcgtcg ccgaccacct cggcatccag
     3001 cgtgtagggc gtgtagtcct cgtcgtccag gaacacgaac gcgtcgccgt ccttgtacga
     3061 ataggtgacc tgccggcgcg agagctcgac gtccttcaac tcgtcgtcgc cgcccacgct
     3121 gatgtccagc ttggtgccgc ccggcacgct gtacatggtg aagcggaact tgacgttgcc
     3181 gccgcggccc tgcgggctgc tgcgctcgat gtcgcggatc tggtagacgc tgccgttgtg
     3241 ttcgacgacg ttacccttct tgatgtcgta agccttcatg ggatgtcttc tatcgggagg
     3301 tttggagggc gcggtagatg cgctcgttga aataccaggt gagcaggccc cagcacaggc
     3361 cgccgatcgc gcacagcggc agggcgacgg cgagcagcag cgggaacctg ggatgcgcag
     3421 cgccgaactg gaccagcatc atgatggcga ggaaggcgaa catcagcccg ccccagaacg
     3481 ccacgccttt cagcagcacg aaacgccaca tgccgcgcgc gcggatcgcc ggccatttct
     3541 gcgcgaccca gcgctccgcg cgcgaaccgt aatcggtcac ttcggcgcca ggcgggtggc
     3601 gccgtccagg cggatggtct cgccgttgag gtaggtgttg ccgaggatgt acccgaccag
     3661 gtcggcgaat tcttccggct tgcccagccg cgacgggaac gggatcgagg ccgccagcga
     3721 ctgctgcacg tcgtccggca tgccatcgac catcggcgtc cagaagatgc cgggtgcgac
     3781 cgtcatcacg cggatgccga aacgggccag ttcgcgcgcc atcggcaggg tcatgccgac
     3841 cacgccaccc ttggatgccg aatacgcggc ctggccgatc tggccttcgt acgccgcaac
     3901 cgacgcggtg ttgacgatca cgccacgctc gccgtcgaca cccgcttcgt tgccctgcat
     3961 gcgttcggcg caggccttgg ccacgttgaa gctgccgacc aggttgacca tcaccgtggc
     4021 ctggaactgc gacagcttca tcgcgccatc gcggcccagc acgcggccgg caccgaggat
     4081 gccggcgcag ttgatcgcga cgttcaggcc acccaggaag tccctggcgg cgtcgacgtt
     4141 ggcaatgacg ccggcttcgt cggtgacgtc ggtgcggaag tagcgcgcgt tcgcttcgcc
     4201 cagttcggcc actgcggccg cgcccttgtc gtcgttgacg tcgaacagcg cgaccttgcc
     4261 gccgtgcgcg accaggtgcc gtgccaccgc gaagccgagg ccggagacgc cgccggtgat
     4321 gacggcacgg gcgttggaga gttgcatggg gagttcctgc gggcggaaac ccgcgatttt
     4381 accgaaacag cggggagcga cgggatgggc gccgggctgc gaagcgtgcg ccatgggtga
     4441 gggcgcgccg gatcgcgcgg catcgccgcg cggcgtgcgc gaacgcccgg cacgctgtgc
     4501 cgggcagggt cggcttgccg agcgcacgcc ggcgatatcg aacaggccgt tcgcatcgag
     4561 gcgcgagcag cccccaagcg cccatccggt cgcgggctat cccgaagcca ggtccagcag
     4621 atagaacgta ttgcaaccac catggccggt cgcacccatc ggcccgtcga tccggcggaa
     4681 accgctgcgt tcgtacagcc gcatcgccgc atccatgccg gtcagggttt ccaggtagca
     4741 ctggtggaat cccgcatccc gcgcggcctg caggcagcgc gccatcatcg ccgcgccggc
     4801 gccgatcccg cgcgcctccg ggaggaaata catcttgcgc agctcgcagg tctcgccgct
     4861 atcgccggcc agcggcgcca ccccggcacc gccgaggatg cggccctcgc gttcgaccac
     4921 gaagtacgca tgccgcggtt cggcgtaggc acgcgacatc cagtccacct ccgggtcgct
     4981 gatcgcgaag ccgcaaccgg tcgcgccgaa ttccggcatc accgtgcgga tgatcgcggc
     5041 catcgcggcg tcgtcatccg ctgtgatcgg gcgtatcgag gtgcctgcgt ggagcgccat
     5101 tttcagtcct gttgcgcgat gacgcgcttg gcgaattcgc ccggcaacaa cccgggcgcg
     5161 aacaggaacc cctggccaac cttgacccca agacggatca ggaagtcgcg ctgggattcg
     5221 gtttcgatgc cctcggcgac caggcccagg cccaggctgc tggcgatgcc ggcgacggcc
     5281 tggcagatcg ccacgtcgga cggtttgccc ggcacgccct tgatgaaggt ctggctgagc
     5341 ttgagcgcat ggatcggcag gcgacgcagg tagttgagtg cgctgtagcc ctcgccgaaa
     5401 tcgtcgatcg acaactgcac gccgagccgg cgcaggcggt cgaacacctc gagcgaatcc
     5461 gccgcgtcat cgaccagcac gcgctcggtc agttccagtt ccagcgcatg cccgggcagg
     5521 ccagcgtcct gcagcacgcg ttgcacgccc tggccgaagt cctcggccag gaattgccgg
     5581 taggacacgt tcaccgcgac ccgctggatc ggcaggtcct gttcgcgcca gcggcgcatc
     5641 tgcgcacagg cttcctgcag cacccattgg ccgatgcgga tgatgtcgcc ggtggtctcc
     5701 gccagctcga tgaagcggtc cgggcgcatt tccccgagtt gcgggttgcg ccagcgcacc
     5761 agcgcctctg catagaggat gcggccggtg cgcaggtcga cctgcggctg gtagaccagg
     5821 tggaactcgc ccttgtctag cgcgcggcgc agctgggtct cggtctgcag ccgctcgtgc
     5881 tgccggctgg ccagctccgg cgagaacgac tggaagccgt tgcgctgccg gcgcttgctg
     5941 tcgtacatcg ccacgtcggc gctctggatc agctggttcg gggtctcgcc atcgtccggc
     6001 gcgcgggcga tgccgatgct ggtggtgacg ttgaactcgt ccttgcccaa ccggaacggt
     6061 tcgccgaagg cctgctggat ctgcttcgcc aaccgttgcg ggcgttcgtg gtcgtcgtcg
     6121 gcgcggcagg ccaccaggaa ctcgtcgccg ccgaaacgcg cgatcaggcc ctcgctaccg
     6181 accgcgccgg cgatgcgttg cgcggcatcg accagcaggg tgtcgccggc gttgtggcca
     6241 agcacgtcgt tgacgatctt gaagcgatcc aggtcgatgt acagcaggca caggccggac
     6301 cgccccgggt tgcgcatgcg cagggtgatc tgttccagga tcgcgtcgcg gttcatcagg
     6361 ccggtgaggg catcggtctg cgcctgcagg cgcaacgcat gttcggcgtg cttgcgctcg
     6421 gtgatgtcct gcagggtccc gctgaggcgg tcgctgccgg gatcgtgcgg gtcgggttcg
     6481 ccgatcatgc ggacccagaa cggactgccg tcggcctgcg cgccctgcag ttccagctcg
     6541 aagccggtgc cgtcgcagat cgattccagg gtgcggcgca ggcgctcgcg gtcgtcctcg
     6601 cgcaggcagg ccagcacctg ctccatggcg tcgagggcct gtgccgcgcc caggatccgg
     6661 ctggcctcgc gggtcaggta caaggcgccg ctggcgcggt cccattgcca gccaccgatc
     6721 tgcgccagtt cctgcacgcg gtcgaacagc gccttgtcgc gttgcaactc ggtgatgtcg
     6781 ctgctcaggg tgagcaccat gtccgcctgg tcggcacccg gcgcgaactg cggtaccgcc
     6841 gtcaccgaca accacagcag gcgctgcgtg ctgcgccgga acaggccgag cacctggctg
     6901 ctgaccgcct gcccggtggc cagcgcccgc gaggccggat gcgcctcgaa cggaaggaag
     6961 cgaccgtcct catcgaccac cagccagtcg ttccagtccg gctcgacgtg gatggactcg
     7021 tcgccgccga tgccgtagat ccggctggcg gcggcgttca tgtaatgcac gcggccctgc
     7081 cggtcctgca gggtgatcgc cttgtcgatg ccctcgatca ggctgttgta gcggacctcg
     7141 gcgtcgtggc gcgcgctgag gtcgcgggcc accgccacca tcgcgtcctg gccgtcatag
     7201 agcaggttgg ccgaatgcac ctccaccggg aagcgggtgc cgtcggcgcg catgttggtg
     7261 acctcgatga cgtaggtctc gccgcggtcg agcgtctcca gcaccggcac caggtggtcg
     7321 cgcggcaggt ccgggttcag cacttcgatc ggctggccga tgatgtcctc gcgcggccgg
     7381 ttgtaggcgc gcatgccggc cttgttgagg tcgagcacgg tgccgtcgcg ggcgagcacg
     7441 ctgaccgggt ccggcaccgc atcgaacagg gcgcggtagc gcaggttgcc ggcctcgatt
     7501 gccgcctgcg cgccgtccag cgcgtcgccc gcgcgcagga gcagggcatg cagcgcgggc
     7561 gagatgcctg ggcctgcggc ggcagcgcgc agggcctgcg cgagtgcatc ggtgctggtc
     7621 gatgccatgt ccgatggggc gggaagcggc ggcacggacg gcgcgttcat ccggcgccca
     7681 gggccttgcc gaccttgctg ccggtgtcgc gtcccagcag gccggccagc cagcggccgg
     7741 tctcggccag cagcgggagg tcgatgccgg tgcggatgcc taaaccgtgg agcatgtaga
     7801 ccacgtcctc gctggccacg ttgccgctgg cgcccttggc gtaggggcag ccgccggcgc
     7861 cggacaccgc gctgtccacc accgccacgc cttcctccag gcaggccagc acgttggcca
     7921 gcgcctgtcc gtaggtgtcg tggaaatgga tcgccagcgc cgccatcggc acctcggcgg
     7981 cgaccgcttt cagcatcgcg cgcgccttcg ccggcgtgcc gacgccgatg gtgtcgccca
     8041 gcgagacctc gtagcagccc atcgcgtgca ggtcgcgggc gacgcgcacc acgtcggcca
     8101 gcggcacctc gccctggtag gggcagccga gcacggtgga gacgtagccg cgcacgcgca
     8161 cgccatccgc gttcgcgcgt tgcaggaccg ggcggaagcg ctccagcgac tgcgccacgg
     8221 tggcattggt gttcctgagg ttgaaggcct cggaggccgc ggtgaacacc gcgacttcgg
     8281 ccgcgccggc cgcgcgcgcg cgttcgtagc cctgttcgtt cggcaccagc accggatagg
     8341 aaatccccgg cgcgcgggtg atgccggcca gtacttcggc ggcatcggcc atctgcggca
     8401 cccatttcgg gctgacgaaa gcggtggcct cgatgctgcg caggccggtg cgcgacagcc
     8461 ggtcgatcag ttcgatcttg gccgtcgtgc cgatatcgac cgactcgttc tgcaggccat
     8521 cgcgcggacc gacctcgacg atgcggacct cggcaggaaa cgtcatgtca gttgccatcg
     8581 gttgcgggtg gcgtgggctg ggccgaggcc gcatcgacag ggacgcgccg cagcaccatc
     8641 agcggctcgc gcacgaacaa tgcggggtat ttgcgcagca atttcgcgat gcggtcggga
     8701 ttgcctgcga tgaaattgtt gtacgtcggc tccttcgtct tcaggccctt ggtgtcgggt
     8761 cgcatgctgt acccgtcgat cttcttcgcc ttgatggcgg cggcgatcca cgcgtggtcg
     8821 gcgtcgtaca ggcgcatcgt gtcgccatcg cgttcgtagc gcagcagcgc tgcgccgcag
     8881 cgcgtgttgc ccgggccgac gcagtccggc ttgccggagt cgttgtgcag ctgcaggtac
     8941 tggccagcgc gggtgtcgat caaggtcagg cggatcggct tggtttcgcc cttggtggtc
     9001 gcgctccggc agtcgggcgc gatctccacc acgccttgcg gtttcatcgg gtcgtcggcg
     9061 gtgccgaccg gttcccagcg gccgggccag gccggatcgc agtcggtggt ggtgccggtg
     9121 ggcagggact cgaagacagt cgtcgaacag ccgcccaagc acagcgacag gagcagcagg
     9181 gccaggcgcg cggttcgttg catcgtcatt gctccagcgt ggccagcacg gcatcgccgt
     9241 cgacgaagtc gccggcctgc gcgcgcaact cggccatcac gccgtcgcgc ggggccttga
     9301 gcgccagttc catcttcatc gcctcgatca ccagcaccac gtcccccgcg gcgaccgggt
     9361 cgcccggctg cttctgcacc accaccacgc ggcccggcat cggtgcgcgg atgcggtgtt
     9421 cggcgctgcc cgcatcgcca gccgcgcgac gatacaccgc ctcggcgtgc aggcgcaggc
     9481 gttgccggcc atcgtggacc agcacgtcgt cgccttcctg catcgcatgg aaccggcgca
     9541 tcccgccgtc gatgcgtgcg ctcagggcgt cgccgtcgaa acgggcgccg gacaccgcgt
     9601 gcgatccgcc atcgacttcg atcgtgtagt cgccgccatg gccccgcgcg accagttcga
     9661 tccgcgccgc gccatcgacg aatgccagtc gccggctgcc gccgtggccc agccgccagc
     9721 catcggcgat ggcccatggc gaacccgcgt cgtggctgcc tgcggcggcg gctcgcgcct
     9781 gcgcttcctg cgccagcaag cgggtggtgg ccgcggcgat cagcagggtg cggtcggtct
     9841 gcgcaggcga caggaattcg tcgaggtggc gatccaggta gccggtgtcg atgctgccct
     9901 cgaccaccgc gggatgccgc accagccgct cgaggaacgc gatgttggac ttggggccgg
     9961 tgatgtcgca ggccgccagc gcctcgcgca agcgggccag cgcgcgcggg cgatcctcgt
    10021 cccagacgat cagcttggcg atcatcgggt cgtagaagat cgtgacggtg tcgccttcga
    10081 ccacgccgga gtcgatccgc acatggcgcg aggcggccgg cagccgcaac gtctgcaacg
    10141 tgccggagcc gggcaggaag ccggcatccg gatcctccgc gtacaaccgc acttcgatgg
    10201 cgtggccgtg ctgccggatc tgttcctgcg ccagcggcag ttgttcgccg gcggcgatgc
    10261 gcaactgcca ttccaccaaa tccagcccgg tgacttcctc ggtgaccgga tgctcgacct
    10321 gcaggcgggt gttgatttcc atgaagtaga aaccgccatc ggcaccgacg atgaattcca
    10381 ccgtgccggc attcgcgtaa tcgatcgcgt gcgcggcctg cacggcggcg gcacccatgc
    10441 gtgcacgcag ttccggggtg aggaagaccg aaggcgattc ctccagcacc ttctggtagc
    10501 gacgctgcgc cgagcattcg cgctcgttga ggtggatcgc cccgccgtgc gaatcgccga
    10561 acacctggat ctcgatgtgc cgcggatgct cgatgtagcg ctccagcagc acgcggtcgc
    10621 gaccgaaggc gttgcgcgcc tcgcgctggc agctttccag gttcgccagg aactcgtcgc
    10681 gcgtgcggac gatgcgcatg cccttgccgc cgccgccgtg cgcggccttg atcatcagcg
    10741 ggtagccgat cttgtcggcc tcgcgctgga gcaggtcggg cgactggtct tcgccggtgt
    10801 aacccggtac gaccggcacg ccggccgcgg ccatcaggtc cttggccccc gccttgctgc
    10861 ccatcttgcg catcgacgcg ccggacgggc cgatgaaggc gatcccggcg gcgagcactg
    10921 cgtcggcgaa gtcggcgttc tcggacagga acccgtaacc gggatggatc gcctgcgcac
    10981 cggtctgctt cgcgacctcg atgatcgcgt cgccgcgcag gtagctgtcc tgcgggcgcg
    11041 cgccgccgat gtggtgcgct tcgtcggcca ggcgcacgtg ctgtgcatcg gcatcggcat
    11101 cggagaacac cgcgacggtg cggatgccga gcctgcgcgc ggtgcggatc acccggcagg
    11161 cgatctcgcc ccgattggcg atgaggatgg tgtcgaacat cagtcttgca cccagtgcgg
    11221 cttgcgcttg tcgaggaagg cggacaggcc ttcctggccc tccggcgata cccgcaggcg
    11281 ggcgatcagg ctggcattgt cggcgtcgtg ccttgcgcca tcggcatgcg cgcagacatc
    11341 gcggaccagc ttcttcgcat tggcgctggc gatcgggccg gctttcagca acagatcgat
    11401 ttgccgctgc acggcggtgt ccagcgcggt cgggttgacc acgtcgtgca ccaagccgat
    11461 gcgcagggct tcatccgtgt cgaagatttc cgccgtcgcg aaatagcgcc gtgcattgcg
    11521 tgcgccgatg gcggcgatga catacggcga gatcaccgcc ggcagcaacc cgagcctgct
    11581 ttcggtcagg ccgaatttcg cgccacgcgc ggcgatggcg atgtcgcagc aggccaccag
    11641 gcccacgccg ccaccgaagg cggagccctg cacggcggcg atggtcggct tcggcaattc
    11701 gtccagcgtg cgcatcaggc gggcgagggc gagcgaatcg gtgcggttct cggtctcgct
    11761 ggcggcggcc atgccgcgca tccagtggag gtcggcgccg gcggagaacg atgcgccttc
    11821 ggcctccagc accagcaccc ggatcgcggc gtcggcggcg acctcatcca gcgctgccgt
    11881 gaggccggcg atcagcgcgg catcgaaggc gttgtggacc tcggcgcggg tcatccgcag
    11941 gcgggcgacg gggccgtcgc ggtgcagttg caaggggtcg ctcatgcggg tccggcaggc
    12001 ctggcgcggg tgaatgcgca tgataaccgg cggcgcgcac cagtccgccg ggcagggcac
    12061 gatgctacaa tgcgaacaat tcccatttgg cttaagcgcg ccggcgaccg atgaccctga
    12121 ccgaactcgc caggcgcacg ccggccatcg tcgacgccgt cgaagatcgc acgccgaacg
    12181 acccgatcgc gcgtcgtttg cgcgagctgg gtttcgtcgc cggcgaggag gtggtggtgg
    12241 tcgcggccgg tccgctcggc gcggaaccgt tgctggtgca ggtcggcttc accaggttcg
    12301 ccctgcgccg cgtcgaggcc gcgcgggtgc gcctgcgcag caatggggca ggcgcatgaa
    12361 cgccgctgtc gcagcgccac gcatcgccct cgtcggcaat ccgaactgcg gcaagaccgc
    12421 gttgttcaac ctgctcaccg gcagccggca gaaggtcgcc aactacgccg gcgtgaccgt
    12481 ggagcgcaag gaagggcgtt tgcatgcgcc gtccggcaag cactacgccg tgctcgattt
    12541 gccgggtgcc tacagcctga atgccgccag cctggacgag gcggtgacgc gcgacctcat
    12601 tcgcggcttc tatcccggcg aagccgcacc ggacctgctg gtctgcgtgg tcgacgccac
    12661 caacctgcgc ctgcacctgc gcttcgtgct ggagctgcgc gcgctgggca agcccatgct
    12721 ggtcgcgctg aacatggccg atgcggcgaa gaaacgcggc atcgacatcg acgtggccgc
    12781 gttgcagcgc gaactgggcg tgccggtggt cgaaaccgtg gccgtgcgtc gcggcggcgc
    12841 cgaccgcctg ctcgccacgc tcgatgcggt ggtcgcgcat ccgcatgccg tcgcggatgc
    12901 gttgccggaa ggcaccgacg tgcacgccga aacccgccgg ctgctggcgg cgacggtgcg
    12961 catgccggag cgcacggcga agatcgacga cgcgctcgac cgctggctgc tgcatccggt
    13021 gttcggtttg tcgacgctcg cggtggtgat gttcctgatc ttccaggcgg tctatgcctg
    13081 ggccacccca gtgatggatc tgatcgaagc cggcaccacg gcgttgggcg catgggccgg
    13141 gacgatgctg ccggcaggac cgctcaacag cctggtggtg gaaggcatca tcgccgggtt
    13201 gggcggggtg atcgtgttcc tgccgcagat cctggtgctg ttcgccttca tcctcgcctt
    13261 ggaggaatcc ggctacctgc cgcgcgcggc gttcctgctg gatcggatga tggccggcgc
    13321 cggcctgtcc ggacgcagct tcatcccgct gctttcgagt ttcgcctgcg cgatccccgg
    13381 gatcatggcc acccgcagca tccaggatcc gcgcgaccgc ctggccacga tcctggtcgc
    13441 gccgctgatg acctgctcgg cgcgcctgcc ggtgtatgcc ctgttgatcg gcgccttcat
    13501 cccgtcgcgc gaggtctggg ccggcatcaa cctgcagggc ttggtgctgt tcgcgctcta
    13561 catggccggg atcttcagcg cgctggcggt gtcgtgggtg atgaagaagt ggcgccgcga
    13621 caaaggcgag cacccgctga tgctggaact gccctcgtac cgcctgccga atccgcgcga
    13681 cgtcctcatc ggcctgtggg aacgcgcgct gatcttcctc aggcgcgtgg gcgggatcat
    13741 cttcgcgctg accgtgctgc tgtgggtcct gctgagcttc ccggccgcgc cggtggacgc
    13801 cacgatgccg gcgatcgact acagcttcgc cggccgcatc ggccatgcga tgaccgcggt
    13861 gttcgcgccg ctgggcttca actggcagat ctgcatcgcg ctgatcccgg ggctggcggc
    13921 gcgcgaagtc gcggtgtcct cattggccac ggtctacgcg ctgtccgctg tcgacgacga
    13981 ggccgccgcg caggccttga ccccgctgat caccaatggc tggtcgctgg ccaccggtct
    14041 ctcgctgctg gtgtggttca tctacgcgcc gcaatgcctg gccacgtggg cggcgatcaa
    14101 gcgcgagacc gggtcttgga agaccatggc gatggccgcc ggttacctgt tcgcgctggc
    14161 gtacctggcc tcgttcgcga cctaccacgt cgcggtggcg ctgggcggag gttgagcgat
    14221 ggcatcgggc ctgtacgcgc aatacgcggt gatcgtgctg gtcgtgatgg cgagcgtgct
    14281 gatcgtgatg cgctcgcagt tcccggcggc cacccgcaag ctgcgcatcg ccatggcgtt
    14341 gccgttgctg cgcgaaggca cgcctgcctg gatgaaagaa cttggtcggc gcatcgcgcc
    14401 gaagccgcgt ggtggggtga acgcctgcgg cggttgcgac aactgcgact agcggcggct
    14461 gccggcgtgc gccgggcgaa tgccaatgga tcctagtgcc tcaggcggcg cttgccgggc
    14521 gaggcggtca cggcttccac gctgacttcg aagctgcgcg attcgccggg gaagaccgcg
    14581 cccacgccgg ccacgtggcg gccgatttcc tcgccgcgtt cattgcggat gacgacgacc
    14641 atggaatatt cgaatgcgcc gtcatccggc gcactcaccg tgccttcgac cttcagcggc
    14701 acgaagcgct gggtggcgtc gctggccggg gcatcgaatt tcagatgccc gcggcacgcc
    14761 gggcagaccg acgagctttc caggatcgtg gccttgcaat gggggcaggt gcgggtcgca
    14821 cctgtcgtgc ccgggcgaac ggcgctcatg cggcgccggc gctctccgtc ttctcggtct
    14881 tggcagtcga cttgccgtcc ttgtcccaac cgcattcgac gtggccgcgg atgcgcgagc
    14941 ccgccgcgac agtcagggtg ccggccttga ggtcgccgtt gatcacgccg ctctgctgca
    15001 gttcgacctg ctgggcggaa tggatgttgc cgtcgagttc gccggccagg gtgaccttgt
    15061 tcgcggccac gccgccggag accttggcgc cgggttcgat ggtgaggttg ccctggacct
    15121 tgacgtcgcc cttgaacttg ccggcgatgc ggacatggcc gctgccttcg atcttgcctt
    15181 cgatggtgag gtcggcggcg atcaccgatt cgcgggccgc cgcgctgtcg actgcgcgga
    15241 ccggcgccgg tgcggcaggt gcggcgaacg gctcgggcgc gggagccggg ctcggcgtgg
    15301 gagccggcga agccaggtcg aagcgggacg gttcattggc cttggcggcc ggggtgggat
    15361 ctttccagat ggccatcgaa caggactcct cgggggcggg tcatcgaggc tagcaccgat
    15421 ttgcggcgat ttcctgcatg aatacgggag gtagatgctg caagggattc accggattgc
    15481 ggttttgtgg atcaggcgcc ggttttgccg cgcatcagga ccagcaccag cagcacggcc
    15541 agcaatgcac cctggacctg gaatatccgc cacagcgcgc cggtctgcgt gcgcgcccga
    15601 accagcgcga tgccgcgctt gcgggcaagc aggacatcgt ggatggcctg cgatccgaac
    15661 gcatataccg cggcgatgca ggcgagatag cggatttcga tgctgtcgcc aacaagttga
    15721 tggaggacga gcagggcaag cccgtaaccg gtcgcgcccg cggcgaggga cgcgccctgg
    15781 ctggcgctgc tgccacgttc gccatccttg cgcgcgccga tccagagcac cgtcgtcatg
    15841 atgaaagcga gggtgccatt ggcgccggcc gcgaatgcca ccacgccgca cgccaagcca
    15901 ccagccgtca tcaacaggac ccagtcccat tgcatgcgcg aatggttgta gtcgcggaaa
    15961 cgaatgaggc ccagcacgac gaccgcatag gcgagccaga ggccatacgg ccaggcgaac
    16021 aaggtcgccc aacgggtcgg ggactcggca tcgaacccgg tcttttcgtc gagcatgctg
    16081 acggcgatgc cgatggcgga tgcccagagg gccaggcgta cggccatcac cgacaggcgc
    16141 tgccaacgca gggcgcggcg atccgttgcg cgttcccaga atcgccgtga cggcgggtcg
    16201 agacgcgtcg aggccgacac ggggtccgcc gcctgcagcg cctgcaggag tgcgccggtc
    16261 cgccccggca gtcccgggca cgcgatcagg cagagtcgac gccaccacga aaacggctgg
    16321 agcagttccc gggcgatgcc ctggtcggcc caggtcgcat ccggaccggc atgcgtgcgc
    16381 aacacgcgct cgaaatccgc tgcatcgccg aagcgctgct ggatggcgtc gagtcggtgg
    16441 tgcagccagc tgtcggtgcc ggagaccgtg tccatcccga agaatcgggt gacggtgtcg
    16501 aaatggcgcg cggcggcctg cgcatcgagc gtctccagca tatcgaccac ggacgcctgc
    16561 agggcacgct ttcgatccag ggcatacagg tcatcgtgcg cctgcagcca ggtttccacc
    16621 gcctgcggcg tttcgctgcg cagccgcgtg cccaggtcgt gggcaaacgc ttctgcatcg
    16681 aagaagtccg acacgcgcag gtcgatgccg tcgcttgcgg gatcaccggg ttcgcgcggg
    16741 atcgtggcga cgtccaaggg cgcaagcagc atgccgacgg aggcgttgcc tgcggcggga
    16801 tcggccacgc catcatcggc atccggttcc acggcgatgc cgtcgtcatc ctcgtcttcg
    16861 tcctcccatc cctgctcgcg ccaacgcagt tgctccaggc aggcctggta ggcctcgtgc
    16921 acgcgctgga agccggtggg gtcctcgtcc gggcggtgcg tgcgcagcag cttcgcgtat
    16981 gcgcgcttga cctgggcgag gtcggccttg cgatccaggc cgaggatgtt gaaaggcgac
    17041 atcggtcagt cgaagccccg cgcctcgatc tggtcgagcg cgcgcgacag ttcgccgcgt
    17101 tcgcgttcga tgcgggcgcg atcctgcgat tcgagcacgc cggtgaagcg tgccagccag
    17161 tcctgcagca ggctccgctg gtccaggcgt tcctcgtaca ggcgctcgat gcgggcgatg
    17221 gtggccaggt tggcctggtc ctcgcgcggg tgcatcttga gcgccgacag cgatgcaagg
    17281 cgttcgcgga tctcggcctc gtcgagtacg ccggggttgt cctgcaggat cagctcgcgc
    17341 ttctcgccgg tgcccagcgt ggtcgattcc acctgcagca cgccattgat gtcgtaggtg
    17401 aagcgaacct cgatcgccgc ctggttcttc tgcatcttcg gtgccaggcg gatgtccagg
    17461 gtgcccagca gcacgttgtt ggccacccgc gggctttcgc cctggaagat ctccagcttg
    17521 atctcgcgct ggccatcgtg catcgcgcag aactgctccg agcgactgac cggcaccgtg
    17581 gcattgcgct cgatgatcgg cgagaaatgc ccgctgctga cccgcccgtg ttcgtcctcg
    17641 cgcgcggtcg cgatgcccaa cgtgtacggg cacacgtcgg tcatgaccac ttcttccagc
    17701 gcctggtcgc gcgccttcat gcccgccgcc acgctggcgc ccagggcgat ggcctggtcc
    17761 ggatgcacat ggcgcagcgg cagcctgcca agcatgcgcg agacgagctt ggtgaagacc
    17821 ggcatccgcg aggcgccgcc gaccatcacc acttcgctga gatcgcccgg cgacagcttc
    17881 gcatcgcgca atgcgcgttc gatcggtgcg cgcacgcgtt gcagcaacgg ttctgcaaga
    17941 cgctggaagc cgacgtcatc gatccgccac tcgcgcgcgg tcccgcccga cgccacgttc
    18001 accaccgcct cggttgccga cgagagctgc ttcttggcca cttccagtcg cgacagcagc
    18061 agggcgagct cgtttggcgg caaggcgcgc cggttgatgc cgagctccct gcaacaggcg
    18121 tcggccatca cgtccaggaa atcctcgccg ccgaggtggt tgtcgcccgc actggcgtgc
    18181 acctgcatga ccccgtcgaa gatctcgagg atcgagacgt cgaacgtgcc accgccgagg
    18241 tcgagcacga tgaaggtcga cccatcgatg cgttcctgca acccgtaggc gagtgccgcc
    18301 gcggtgggtt cgttgaccag ccgctcgacc ttgatgccgg ccagctcgcc ggcggcacgc
    18361 gtggccttgc gttgcgcgtc gccaaaatag gccgggacgc tgatcaccgc ctcgcggacc
    18421 ttttcgccaa gggcggcctc ggcatccgcc aacagggagc gcaggaccag cgccgacaat
    18481 tcctcgggac ggaattcgcg cttgcccagt cgggtgctgc ggttggatcc catccagcgc
    18541 ttgaacgcgg ccacgctggc ctgcggatgc gtgatgagcc ggtcgcgcgc ggattggccc
    18601 accaggatgc cgccgtcttc gccgatgctc accaccgatg gtgtcagcag gtcgccgagc
    18661 gcgttcggga acaggcgggg tgcgttgcct tcgaaacagc cgatgaggga gtgggtggtg
    18721 cccaggtcga tgccgacgat catccttgaa tccgattccc tggtggttac atgcggaaca
    18781 cgccgaagcg ggtgcgatcc tcgatgggtg cattgagcga tgccgacagc cccagtccca
    18841 gcacgcggcg ggtgtctgcg ggatcgatga tgccgtcatc ccagagccgc gcgctggcgt
    18901 agtaggggtt gccctgggtt tcgtactgct cgcggatcgg tgccttgaat gcgtcttctt
    18961 cctcggcgct ccaacgtcca cccttgagtt cgatgccgtc gcgcttcacg gtcgccagca
    19021 cgctggccgc ctgttcgccg cccatcacgc tgatgcgcgc gttcggccac atccacagga
    19081 agcgcgcgcc gtaggcgcgg ccgcacatgg cgtagtt
//
LOCUS       DAOOCX010000041        18757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2595_length_18757_cov_9.812854, whole genome shotgun sequence.
ACCESSION   DAOOCX010000041 DAOOCX010000000
VERSION     DAOOCX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 18757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18757
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2595_length_18757_cov_9.812854"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK399_05695"
     CDS             <1..186
                     /locus_tag="PK399_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12645.1"
                     /translation="LQHEADDQDHGCAGHGAGHLGLSIAVLCRSAGRSNWPVAADNPV
                     TRSFYTASVDIRRSREV"
     gene            complement(195..>1373)
                     /locus_tag="PK399_05700"
     CDS             complement(195..>1373)
                     /locus_tag="PK399_05700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPW12646.1"
                     /translation="DYKRVERELREVNETLEQRVEQRTREAEAAQQSKTRFLAAISHD
                     VLQPLHAARLFTSAMHEGGDAEEQARLASRVDASLRAAEELLDGLLDVSRLDAGVLQP
                     QWSDFDATELLRELQAQYAPTAAQRGLALRIHAPEVLPVRSDRRLLRRALQNFIGNAL
                     RYAQSGGVLLAARRRGDGVSLEVWDTGPGIPANHLEQIFEEFHRYDQPGERGQRGLGL
                     GLSICQRISAVLNHPLHVRSSVGRGSVFGIRVPRASAPLRHDTSPRMAIESHDAALHG
                     LRVLCVDNDPEILAGMRALLGRWGVEVSCASTVDDALQLAGDGPEVLLVDYHLHDRLD
                     GLDTLDALRAACGDVPGALLTADGSDVLKRNARLRGYRVLTKPIKPASLRAFLASVRS
                     "
     assembly_gap    1374..1473
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1474..3470)
                     /locus_tag="PK399_05705"
     CDS             complement(<1474..3470)
                     /locus_tag="PK399_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPW12647.1"
                     /translation="MMPGWLLLLVALAYVGLLFTVAWLGERHPGLAQGPRLRPAVYAL
                     ALAVYCSSWTFYGAVGTAARAGLGFLPIYLGPILLLLFGWRLLERLVLASGKHSIVSI
                     SDFLSSRYGRARGLAAVVAVIAVIAAIPYFALQFKAVGMSVGVLSGQPPAAQWFVDPA
                     LYVALMLAVFAILFGTRRIDATEHHRGMVLAIALESLVKLVAFVAIGLFALWHLPGTD
                     DFTARVAAGLHAFTAPQLPAGFLAQTLLGFLAIICLPRQFHVAVVECQDPADLQPARW
                     MFMGYLALVSAMVLPIALAGQAMPGADAMSADSHVLTLPLAHGHEWLALMAYIGGLSA
                     ATGMVIVASVALSTMVSNDLVVPLLLRRGLLHHGADIEQRVLLIRRIAIIVLALLAFA
                     YHRHALGQQVLAQHGLLAFAAVAQFAPALIGGLYWRGASRTGAYAGLLTGALLWAWCL
                     LLPALLQTDGAPPAWMLDGPFGQRWLRPESLFGLSGWDPLTHGVFWSLLGNIAMFVFM
                     SMRHRPLLQDQLAAADYLDPYAHRPMLAPGGWVGKVRGSELLTLAERILGEQHARRAF
                     QEYASQQQHRWEPEQPADRALVQFTERLLASALGAASARSTLTSVLRGSGMELGEVVS
                     LLDETSHALRFNREILTTTLENIPHGVSVVDADMRLVAWNRR"
     gene            complement(3565..4983)
                     /gene="cls"
                     /locus_tag="PK399_05710"
     CDS             complement(3565..4983)
                     /gene="cls"
                     /locus_tag="PK399_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPW12648.1"
                     /translation="MGIDLHPATWILIADWAIRLAALFWIPARSAPAAARSWLLLVGF
                     VPLLGLPLYLAFGHPWLSAVRRERQARASNVIAERQQPLAPLRWTPHDDSVADDTARL
                     CERTGDFMPMTGNEVRLLADYDASLQALLGDIATARSEVHLLYYLMFDDAVGRAVADA
                     LRAASKRGVRCRLLLDAVGGKRGLRGFTTSLRDAGVDVQAMLAGGLAWRRSARMDLRN
                     HRKLAVIDRCIGHTGSQNLADAEFIRGVPNREVVARVRGPVVAQLGALFASDWFIETG
                     EILSVPEQPPAAEGHTPAQLLPSGPAYPFANARNAVIAMAQRARHRLVLATPYFVPDD
                     ATLDALQIAALSGIDVQLILSRSNNQRLTAWAQDSYIDGLLAAGVKVALHRPHFLHAK
                     HLSVDDEIALVGSINLDIRSFALNAEVGLLCYDKAVVAQLRAIEADCLADADILTAPQ
                     WRQRPAWKRSLSGIARLADSFL"
     gene            complement(5084..6304)
                     /locus_tag="PK399_05715"
     CDS             complement(5084..6304)
                     /locus_tag="PK399_05715"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPW12649.1"
                     /translation="MQTSQFELLHAEGSTTPIKGWVRGVPLEDQAHEQLRNIASIPFV
                     GPWVAVMPDVHLGKGATVGSVIPTRGAIIPAAVGVDIGCGMAAVRTTLRASDLPDDLG
                     PLRNSIERSVPVGNGRGGEHWKLPDSIETRVAQSGLVERLDVIKQKHRKIRTDKLDRQ
                     IGTLGGGNHFIELCLDESDAVWVMLHSGSRGTGNLIGTYFIERAREELAKRVLGFHLP
                     DKDLAFFMEGEPLFDDYVEAVSWAQDYARENREAMMARVLHEMRHRLPKFQLEKMAVN
                     CHHNYVQKETHGDVDLLVTRKGAVSARAGELGIIPGSMGAKSFIVRGLGNADSFHSCS
                     HGAGRVMSRTKARQQITLAQHREATAHVECRKDAGVIDESPAAYKDIDAVMAAQSDLV
                     EIVHTLRQVLCIKG"
     gene            complement(6367..7488)
                     /locus_tag="PK399_05720"
     CDS             complement(6367..7488)
                     /locus_tag="PK399_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005420258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPW12650.1"
                     /translation="MFWTKRVVIGDGERAVLYRDRQFERVLAPGVHRMFDPLKRIEVR
                     TFNIAAPEYAGHDVDALIARLGERLAATFVLADIGADEIGLVSKNGKLEDVLAPGSRR
                     LYWRGLVKVEVERVSLADGLAVRGDVANRLRQLGTLGKLAVVAEVPAESAGLVFIDGK
                     LVQVLEAGSHAFWNFRKNVAVDVIELRVQSLEVSGQELLTRDKVSLRVNLAASIRITD
                     PVAARTRVAKFGDYVYRELQFGLRKAIAAKTLDELLGDKASLDADIFGYVRGRVGELG
                     IEVLGVGVKDVILPGEMKDILNGVVQAEKLAQANVIRRREEANATRSLLNTARLIEES
                     PVLMRLKELEALEKVTEKIGNLTVFGGLDGVMKQLVSLK"
     gene            complement(7887..7954)
                     /locus_tag="PK399_05725"
     tRNA            complement(7887..7954)
                     /locus_tag="PK399_05725"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7921..7923),aa:Asn,seq:gtt)
     gene            complement(7960..8027)
                     /locus_tag="PK399_05730"
     tRNA            complement(7960..8027)
                     /locus_tag="PK399_05730"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(8419..8553)
                     /locus_tag="PK399_05735"
     CDS             complement(8419..8553)
                     /locus_tag="PK399_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12651.1"
                     /translation="MTSVFHIAALLPSPKRDAGLRACERRDALQLRGKRNYFGTTHID
                     "
     assembly_gap    8788..8887
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9015..9179)
                     /gene="rpmG"
                     /locus_tag="PK399_05740"
     CDS             complement(9015..9179)
                     /gene="rpmG"
                     /locus_tag="PK399_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPW12652.1"
                     /translation="MATKRDKIRLISSAGTGHFYTTDKNKKNTPAKMEVSKYDPVVRK
                     HVMYKEGKIK"
     gene            complement(9193..9429)
                     /gene="rpmB"
                     /locus_tag="PK399_05745"
     CDS             complement(9193..9429)
                     /gene="rpmB"
                     /locus_tag="PK399_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPW12653.1"
                     /translation="MSKVCQVTGKRTISGNNVSHSNRKTRRRFMPNLHERRFWVASEN
                     RWVKLRVSAAALRTIDKNGIDAILADLRKQGEKV"
     gene            complement(9781..11526)
                     /locus_tag="PK399_05750"
     CDS             complement(9781..11526)
                     /locus_tag="PK399_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HPW12654.1"
                     /translation="MKSFVLACCLLLPIAAHAATPPASSPTDKTFEAIYKAEWDWRTQ
                     QAPSWDEDSDNSARTPSTRLADVGPAAQAKRLAYLDGVLRQLDGIDASTLSPANQVNF
                     AVYRPQLEHMAAELRARDYEMPFNADSSFWSDLGFMARADLRDAAAYRAYAARLRDVP
                     RYFDQQTANMRAGLARGFSVPRAVLVGRDGSIAMVAELKDIESSNFYDPYRKMPSTIP
                     PAEQETLRAEGRAAIRDAVVPAFAKLLVFFRSEYMPKARTTLAAEAMPEGEAWYREQI
                     RKYTTLDLSADQIHAIGLKEVASIRAEMDGIIQQVGFKGSFADFLAFLRSDPRFYAKT
                     PQELLDRAAWISKRVDGQIGKLIGTLPRGRFTIVPVPPDIAPFWTAGRGGADTYWVNT
                     YNLPSRPLYNLPALTLHESSPGHALQGSLVREQGDVPDFRKDYISAYGEGWGLYSEWL
                     GKEMGIYETPYEDFGRLTYAMWRACRLVIDTGVHHKGWTREQALAYLRDNTALSEHEV
                     TTEVDRYISWPGQALSYKLGELTIMRLRREAEAALGPKFDVRAFHDAVLAQGSVPLPV
                     LESQIRAYIAEAKAK"
     assembly_gap    11773..11872
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11873..12162)
                     /locus_tag="PK399_05755"
     CDS             complement(<11873..12162)
                     /locus_tag="PK399_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HPW12655.1"
                     /translation="MNSAIPWLEWTGIHMSPWKLIGLVGALMFGGRWLVQFIASKRQG
                     KPVIPRLFWYMSILGSLMTLAYFIFGKNDAVGILQNLFPSFTACYSLYLDIK"
     gene            complement(12183..12929)
                     /locus_tag="PK399_05760"
     CDS             complement(12183..12929)
                     /locus_tag="PK399_05760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW12656.1"
                     /translation="MSASIASGNPQLSVVVPVHNEEDNVTPLVGEIVAALRGVISFEI
                     VYVDDTSRDATLQRLRDLQATTPELRVIRHLSNAGQSTAVRNGVKAARAPWIATLDGD
                     GQNDPADIPKLLAQRDAAAADIKLFAGWRVNRQDSGSKRWASKWANAIRARMLRDDTP
                     DTGCGIKLFEREAFLDLPYFDHMHRYLPALMQRAGWKTVSVPVNHRHRTAGVSKYNNL
                     NRALVGIRDLRGVAWLIVRSRRTAVEELPR"
     gene            complement(12943..13881)
                     /locus_tag="PK399_05765"
     CDS             complement(12943..13881)
                     /locus_tag="PK399_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPW12657.1"
                     /translation="MTAGNQPAKSAAKKRGLGRGLEALLGPKAAAEAPPLQAMPGDTL
                     RTLPVDAMVPGKYQPRRSMDDAKLEELAASIRSQGVIQPIVVREHVGADGKGGRTYEI
                     IAGERRWRASQRAGLAEVPVVVRVVDDRTVVAMALIENIQREDLNPLEEAQALQRLID
                     EFDLTHAAAAEAVGRSRAAVSNLLRLLELPAEIRALVQAGSIEMGHARALLPLAAPMA
                     ISLAKQAAEHGWSVREVEHRVQQLASGKVTAPPKKAAPKARPQADIVTLERELSESLG
                     SRVEVVPGRGKKGKLVIHYADFDVLDGVLDRLRTKP"
     gene            complement(13878..14672)
                     /locus_tag="PK399_05770"
     CDS             complement(13878..14672)
                     /locus_tag="PK399_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW12658.1"
                     /translation="MARIIAIANQKGGVGKTTTAVNLAAALARTPKRVLLVDLDPQGN
                     ATMGSGIEKRELETSITNVMLGEAQAREAIVRCQDGYDLLPGNIDLTAAEIQLMDMPG
                     REQKLKQALDTVRDGYDFVIVDCPPSLSLLTLNALTASDSVLVPMQCEYYALEGLSAL
                     IDTIDALKGRLNPALQIEGVLRTMFDVRNNLANAVSAELTNHFGDLVFRTIIPRNVRL
                     AEAPSHGQSIIGYDKASRGGVAYLGLAGELLRRQRERTQASKEKAA"
     gene            complement(14724..15371)
                     /gene="rsmG"
                     /locus_tag="PK399_05775"
     CDS             complement(14724..15371)
                     /gene="rsmG"
                     /locus_tag="PK399_05775"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPW12659.1"
                     /translation="MSDDMSALRPELEAGLAALSLDLALASPLLAYVALLARWNATYN
                     LTAVRDPREMIAKHLLDSLAMQPFVRGLGTLADLGTGPGLPGIPLAIATPSLQVTLVE
                     SNGKKARFLREAIRQLKLENASVAESRIEAFKPGATFDAITARALATLPLILALGGHL
                     LGTDGRLLAMKGVLPSEEIAALPAGWRLAAAHPLRVPGLVGERHLVEVVRAGSEA"
     gene            15558..15812
                     /locus_tag="PK399_05780"
     CDS             15558..15812
                     /locus_tag="PK399_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011409801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HPW12660.1"
                     /translation="MNFLIYLIVGGIAGWLASIMMKRDGSQGIILNVIVGIIGGFLGG
                     ILLPMVGLSFGGTIGFLITAFIGAVVLLLIVNLFTRGRAR"
     gene            complement(15892..16665)
                     /gene="xth"
                     /locus_tag="PK399_05785"
     CDS             complement(15892..16665)
                     /gene="xth"
                     /locus_tag="PK399_05785"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPW12661.1"
                     /translation="MKIASWNVNSLNVRLPHLQQWLQEFAPDVVGLQETKLEDHKFPE
                     DALLAAGYRSAFVGQKTYNGVAILARHPIGDVQLGIPGFDDAQARAIAATVNGVRIVN
                     LYVVNGQDIGTDKYAYKLRWLDAVHAWVADELQRHPKLVVLGDFNIAPDDRDVHDPMV
                     WNDDHILTSTAERGALRGLLELGLHDAFRLHHDDAGIFSWWDYRQAAFRRDLGLRIDL
                     TLVSDALKADAIQAGIDRTPRTWERASDHTPAWVQLAAG"
     gene            complement(16673..18073)
                     /locus_tag="PK399_05790"
     CDS             complement(16673..18073)
                     /locus_tag="PK399_05790"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HPW12662.1"
                     /translation="MDTPISDLAPTLQRLREAWQANKPDYTQRHADLQRLRDVLKRKL
                     DAMATTIAADFGHRPRAESVVADGMTVLNDIDHLIRHLRRWMKPTRVGVGWRFLPARA
                     QVRPVPVGVVGVIAPWNYPVNLSLIPLATAIAAGNHVYLKPSEHTPKTSQFLRELLAE
                     VFPPDRVVVATGGADIGAAFAGLPFDHLVFTGSTAVGRKVMAAAAPNLTPLTLELGGK
                     SPALIADDYPIERAAARIATGKWFNGGQTCIAPDYVLVPKGKRDALVKALRAEVLARY
                     GEHFENVGDYARIINDGQYARLKGYLDDAKQRGIEKVTLAGHPDPAQRLMPPTLLLDP
                     GDDATVMQEEIFGPLLPIKTYRTLDEAIAYINAHDRPLALYPFSDDKATVEKILHHTL
                     AGGVSVNDTLFHFAITDLPFGGVGPSGMGAYHGRAGFDAMSKQLPVLWQARRTGGDLL
                     KPPYSKAKWLLDLIVR"
     gene            18122..18667
                     /locus_tag="PK399_05795"
     CDS             18122..18667
                     /locus_tag="PK399_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPW12663.1"
                     /translation="MTSNTPVLRPYRDILPTVGQRAYVDPAATVIGDVVLGDDVSIWP
                     GTIVRGDVNFIRIGARTNVQDGSVIHVSHDGPHAKLGGFATVIGEDVTIGHKAIIHAC
                     RIEDAVLVGMGAVVLDGAVVKKHGFVGAGTLIAPGKVVGEGELWLGNPGRKVRMLSDA
                     EIEALYYSAQHYVRLKDHYLA"
BASE COUNT         2941 a         6163 c         6238 g         3115 t
ORIGIN      
        1 ctacaacatg aagcggatga tcaagatcat gggtgcgcag ggcatggtgc gggccatctc
       61 ggcctgagca tcgcggttct ttgccgcagc gccggtcgct caaattggcc cgtcgccgcc
      121 gacaacccag taacgaggag tttttacaca gcctcggtcg atatccgtcg ttcgcgtgag
      181 gtctgaaagt agttttaaga ccgcaccgac gccaaaaacg cccgcaaaga cgcaggcttg
      241 atcggcttcg tcagcacgcg atacccccgc aatcgtgcat tgcgcttcaa cacatcgctg
      301 ccatccgcag taagcaacgc gcccggcaca tcgccgcacg cggcacgcaa cgcatccaat
      361 gtgtcgagtc cgtccaggcg gtcgtgcagg tggtagtcca ccagcaggac ttcgggtcca
      421 tcgccggcga gctgcagcgc atcgtcgacg gtggaggcgc aactcacctc cacgccccag
      481 cgaccgagca gggcgcgcat gccggccagg atttccgggt cgttgtccac gcacagcacg
      541 cgcaggccgt gcagggcggc atcgtggctt tcgatggcca tgcgtggcga cgtgtcatgg
      601 cgcagcggcg cgctggcgcg cggcacgcgg atgccgaaca cgctgccgcg gcccacgctg
      661 gaacgcacgt gcagcggatg gttgagcacg gcggagatgc gctggcagat cgacaatccc
      721 aggcccagtc cgcgctggcc gcgctcaccg ggttggtcgt agcgatggaa ctcctcgaag
      781 atctgctcga ggtggttggc ggggatgccg gggccggtgt cccacacttc cagtgacaca
      841 ccatcgccac gccgccgcgc cgccagcagc acgccgccgc tttgcgcgta acgcagggcg
      901 ttgccgatga aattctgcag cgcgcgacgc aacaggcgac ggtcgctgcg caccggtagg
      961 acctcgggcg cgtggatgcg caacgccagc ccgcgttgcg cggcggtggg cgcgtattgc
     1021 gcctgcaatt cgcgcaggag ttcggtggcg tcgaagtcgc tccattgcgg ctgcagcacg
     1081 ccggcatcga gccgcgagac atccagcaag ccgtccagca gttcctcggc ggcgcgcagc
     1141 gacgcgtcca cgcgcgaggc gagccgcgcc tgttcctcgg catcgccgcc ttcgtgcatc
     1201 gccgaggtga acaggcgcgc cgcatgcagc ggctgcagca cgtcatggct gatcgcggcc
     1261 aggaagcgcg tcttgctctg ctgcgcggcc tcggcctcgc gcgtgcgctg ttcgacgcgt
     1321 tgctccagcg tctcgttgac ctcgcgcagc tcgcgctcca cgcgcttgta gtcnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggcggt tccacgcgac caggcgcatg
     1501 tcggcatcga ccacgctgac gccgtgcggg atgttctcca gcgtggtggt caggatctcg
     1561 cggttgaagc gcagcgcgtg gctggtttca tcgagcaggc tgaccacttc gcccagctcc
     1621 atgccggagc cgcgcaacac cgaggtcagc gtgctgcgcg cggaggcagc gcccagggcc
     1681 gatgccagca agcgctcggt gaattgcacc agcgcgcgat ccgcgggttg ttcgggctcc
     1741 caacgatgct gttgctgcga ggcgtattcc tggaacgcgc ggcgtgcgtg ctgctcgccg
     1801 aggatgcgtt cggccagggt cagcagttcg ctgccgcgca ccttgccgac ccaaccaccc
     1861 ggcgccagca tcgggcgatg cgcgtagggg tccaggtaat cggcggcggc cagctggtct
     1921 tgcagcagcg ggcgatggcg catcgacatg aacacgaaca tcgcgatgtt gcccagcagc
     1981 gaccagaaca cgccatgcgt cagcggatcc cagccgctca gtccgaacag cgattccggg
     2041 cgcagccagc gttgcccgaa cgggccgtcc agcatccacg caggcggcgc accgtcggtc
     2101 tgcagcaggg cgggaagcag caggcaccat gcccacaaca gcgcgcccgt gagcagaccc
     2161 gcatacgcgc cggtgcggct cgcgccacgc cagtacaggc cgccgatcag ggccggcgcg
     2221 aattgcgcca ccgcggcgaa ggcgagcagg ccgtgctgcg ccagcacctg ctggcccagc
     2281 gcatggcggt gataggcgaa ggcgagcaac gcgagcacga tgatcgcgat gcggcggatc
     2341 agcagcacgc gttgttcgat gtcggcgccg tggtgcaaca ggccgcgacg cagcagcagc
     2401 ggcacgacga ggtcgttgct caccatcgtc gacagggcca cgctggccac gatcaccatg
     2461 ccggtcgccg ccgacaggcc gccgatgtag gccatcagcg ccagccattc gtggccatgc
     2521 gcaagcggga gcgtcaacac gtggctgtcg gccgacatcg cgtccgcgcc cggcatcgcc
     2581 tggccggcca gcgcgatcgg caacaccatg gcactgacca gcgcgagata gcccatgaac
     2641 atccagcgcg cgggttgcag gtcggccgga tcctggcact cgaccacggc cacgtggaat
     2701 tgccggggca ggcagatgat ggccaggaag cccagcaagg tctgggccag gaatcccgct
     2761 ggcagttgcg gcgccgtgaa ggcgtgcagg cccgccgcca cccgtgcggt gaagtcgtcg
     2821 gtgccgggca ggtgccacag cgcgaacagg ccgatcgcga cgaacgcgac cagcttgacc
     2881 agcgattcca gcgcgatcgc cagcaccatg ccgcgatggt gctcggtggc atcgatgcgc
     2941 cgcgtgccga acaggatcgc gaacaccgcc agcatcagcg ccacgtacag cgcgggatcg
     3001 acgaaccact gcgcggccgg tggctggccg ctgagcacgc ccacgctcat gcccacggcc
     3061 ttgaactgca gtgcgaaata cgggatcgcc gcgatcaccg cgatcaccgc gaccacggcc
     3121 gccagtccgc gcgcgcggcc atagcgcgac gacaggaaat cggagatcga cacgatgctg
     3181 tgcttgcccg atgcaagcac cagccgttcc agcaggcgcc agccgaacag cagcaacagg
     3241 atcggtccca ggtagatcgg caggaagccc aggcccgccc gcgcggccgt gcccaccgcg
     3301 ccatagaacg tccacgagga gcagtacacc gcgagcgcca gcgcatacac cgccggccgc
     3361 aggcgcgggc cttgcgcaag tcccggatgg cgttcgccca gccaggccac ggtgaacagc
     3421 aggcccacgt aggccagtgc caccagcagc aggagccagc cgggcatcat cgcgtgcgtg
     3481 cgtccttgtt cggggagccc gcagtgtaag caagcgacgc gggcgcgacc cgctgcgatg
     3541 cacgatgccg ccgcatcgcc atcgctacag gaacgagtcg gccagccgcg cgatgccgga
     3601 caagctgcgc ttccatgccg gtcgttgccg ccactgcggc gcggtcagga tgtcggcatc
     3661 ggccaggcaa tcggcttcga tggcgcgcag ctgcgcgacc acggccttgt cgtaacagag
     3721 caggccgact tccgcattga gcgcgaacga gcggatgtcg aggttgatcg acccgaccaa
     3781 ggcgatctcg tcatccacgc tcaagtgctt ggcatggagg aaatgcgggc gatgcagggc
     3841 gaccttgacg ccggccgcga gcaggccgtc gatgtaggaa tcctgcgccc acgcggtgag
     3901 ccgctggttg ttgctgcgcg agaggatcag ctgcacgtcg atgccggaca gcgcggcgat
     3961 ctgcaacgcg tccagggtgg cgtcgtcggg tacgaagtag ggcgtggcca gcaccagccg
     4021 atgccgggcg cgctgcgcca tcgcgatcac cgcgttgcgc gcgttggcga acggatacgc
     4081 cgggccgctg ggcagcagct gcgccggcgt gtggccttcg gcagccggag gttgctcggg
     4141 caccgacagg atctcgccgg tttcgatgaa ccagtcgctt gcgaacagcg caccgagttg
     4201 cgcgaccacc gggccgcgca cgcgcgccac cacttcgcgg tttggcacgc cgcggatgaa
     4261 ttcggcgtcg gccaggttct gcgatccggt atggccgatg cagcggtcga tcaccgccag
     4321 cttgcgatgg ttgcgcaggt ccatgcgtgc actgcgccgc catgccaggc caccggccag
     4381 catcgcctgc acatccacgc cagcgtcgcg cagcgaggtc gtgaagccac gcaggccacg
     4441 cttgccgccg accgcgtcca gcaacaaccg gcaacgaacg ccgcgtttcg atgctgcacg
     4501 tagcgcatcg gcgacggcac gaccgaccgc atcgtcgaac atcaggtagt agagcaggtg
     4561 gacttcgctg cgggccgtgg cgatatcgcc cagcaaggcc tgcagcgagg cgtcgtagtc
     4621 ggcgagcagg cgcacctcgt tgccggtcat cggcatgaag tcgccggtgc gttcgcacaa
     4681 gcgcgcggtg tcgtcggcca cgctgtcgtc gtgtggtgtc cagcgcagcg gcgcgagcgg
     4741 ctgctggcgt tccgcgatga cgttcgatgc gcgcgcctgg cgttcgcgac gcacggccga
     4801 tagccacgga tggccgaagg cgaggtacag cggcaagccc agcagcggca cgaagccgac
     4861 cagcaacaac cagctgcgtg cggccgccgg cgcgctgcgt gccgggatcc agaacagcgc
     4921 agccaggcga atcgcccagt cggcgatcag gatccacgtg gccgggtgca ggtcgatgcc
     4981 catgctcgga ttgcaccatg cacgcgcccg gcgtgcgagc ctcgcgcgca aaaagaaacc
     5041 cgccttgcgg cgggtttcgt ggccatccat ggccggtgtt gcttcagcct ttgatgcaca
     5101 gcacctggcg caaggtgtgc acgatctcca ccaggtcgga ctgcgccgcc atcaccgcgt
     5161 cgatgtcctt gtacgcggcc ggcgactcgt cgatcacgcc ggcgtccttg cggcactcga
     5221 cgtgggcggt cgcctcccgg tgctgcgcca gggtgatctg ctggcgtgcc ttggtgcggc
     5281 tcatcacgcg gccggcgcca tggctgcagc tgtggaagct gtccgcgttg ccgaggccgc
     5341 gcacgatgaa gctcttcgcg cccatgctgc ccgggatgat cccgagctcg cctgcgcgcg
     5401 cgctcaccgc gcccttgcgg gtgaccagca ggtccacgtc gccgtgcgtc tccttctgca
     5461 cgtagttgtg gtggcagttc accgccatct tttccagctg gaacttcggc aggcgatgac
     5521 gcatctcgtg cagcacccgc gccatcatcg cctcgcggtt ctcgcgcgcg tagtcctgcg
     5581 cccacgacac cgcttccacg tagtcgtcga acagcggctc gccttccatg aagaacgcga
     5641 ggtccttgtc cggcagatgg aagcccagca cgcgctttgc caactcctcg cgcgcacgtt
     5701 cgatgaagta cgtgccgatc aggttgccgg tgccgcgcga gccgctgtgc aacatcaccc
     5761 acacggcatc gctctcgtcg aggcagagct cgatgaagtg gttgccgccg cccagcgtgc
     5821 cgatctgccg atcgagcttg tcggtgcgga tcttgcggtg cttctgcttg atcacgtcca
     5881 ggcgctcgac cagccccgat tgcgcgacgc gggtctcgat gctgtccggc aacttccagt
     5941 gctcgccgcc gcggccgttg ccgaccggca cgctgcgctc gatggagttg cgcagcgggc
     6001 cgaggtcgtc gggcaggtcg gaagcgcgca gggtggtgcg caccgcggcc atgccgcagc
     6061 cgatgtcgac accgaccgcg gccgggatga tcgcgccacg ggtcgggatc accgaaccca
     6121 cggtcgcgcc cttgcccagg tgcacgtcgg gcatcaccgc gacccacggc ccgacgaacg
     6181 ggatcgacgc gatgttgcgc agttgttcgt gcgcctggtc ttccagcggc acgccgcgca
     6241 cccatccctt gatcggcgtg gtactgccct cggcgtgcag caattcgaat tgcgaggttt
     6301 gcatgttcat gtcttcagtc ctttgaaaga aacccgcgtg acggatccgt ccgccacgcg
     6361 gggttgttac ttgaggctca ccaactgctt catgacgccg tccaggccgc cgaacacggt
     6421 caggttgccg atcttctcgg tgaccttctc cagcgcctcc agctccttga ggcgcatcag
     6481 caccgggctc tcctcgatca ggcgggcggt gttgagcagg gaacgcgtcg cgttcgcctc
     6541 ctcgcgacga cggatcacgt tggcctgcgc caacttttcc gcctgcacca cgccgttgag
     6601 gatgtccttc atttcgcccg gcaggatcac gtccttcacg cccacgccga gcacctcgat
     6661 gccgagctcg ccgacgcgac cgcgcacgta accgaagatg tcggcgtcga gcgaggcctt
     6721 gtcgcccagc aactcgtcca gcgtcttcgc cgcgatcgcc ttgcgcaggc cgaactgcag
     6781 ctcgcggtac acgtagtcgc cgaacttcgc gaccctggtg cgcgcggcca ccgggtcggt
     6841 gatgcggatg ctcgcggcca ggttcacgcg caggctgacc ttgtcgcggg tcagcaactc
     6901 ctggcccgag acctccagcg actgcacgcg caactcgatc acgtcgaccg ccacgttctt
     6961 gcggaaattc cagaacgcat ggctgcccgc ctccagcacc tgcaccagct tgccgtcgat
     7021 gaacaccagt ccggcggact ccgccggtac ttcggcgacg accgcgagct tgcccagcgt
     7081 gccgagctgg cgcaggcggt tggcgacatc gccgcgcacc gccaagccat cggccagcga
     7141 cacgcgctcc acctcgacct tgaccaggcc acgccagtac agccgacgcg aacccggtgc
     7201 cagcacgtcc tccagcttgc cgttcttcga caccagcccg atctcgtcgg caccgatgtc
     7261 ggcgagcacg aacgtggcgg caagccgctc gccgaggcgc gcgatcagcg catccacgtc
     7321 gtggccggcg tattccggtg cggcgatgtt gaaggtgcgc acctcgatgc gcttgagcgg
     7381 atcgaacatg cgatgcacgc ccggcgccag cacgcgctcg aactggcgat cgcggtagag
     7441 cacggcacgc tcgccgtcgc cgatcacgac cctcttggtc cagaacatgg gaactttcct
     7501 tgttggtcct ggtggtccga tccgatggat gcgcgatgga tcggttctcg cgtttttcac
     7561 gttctggctt ctgtgggagg ggcttcagcc ccgagctctc tggatcgagg ctgaagccgc
     7621 tcctgcgaac agcggaaaag gtggaaacgc ccctgacgcc gatgccgcgg agcctgtgcg
     7681 ctgtccgtgc ggaggcgatg caatcctcgg ttccgcaatc catcgcgaac ccgattcgtg
     7741 caggcccgcg cgcgtcgagg gcggcggccc ggcgatacca ccgctgcgtg tccgttatcg
     7801 ttgcgatgcg ctgcaacgtt tcggaacacg cacggggcgt ttcctggtgt gggcttgcgc
     7861 ccggattgcc agatttacag tctggtttgg agcgggaatc gaacccgcaa cagtcggatt
     7921 aaccgtccga tgctctaacc gattgagcta tccatgggag acgtaccgga ttcgaaccgg
     7981 tgacctgcag tcatgcctgc tgctctagcc aactgagcta acgccatttc gtggaacatc
     8041 gtctcccgat ccccggcata cgccacggca gtgccgcgcg caccggacgg gcatgcaagc
     8101 ccgtaccgct gtggagcgtt cgtcgatgtt ccgcattggc gcctccgaca cgacatcgaa
     8161 agcgccaatc cgtgtgcgat cgcatccgct ttccgcccgt gccgaagcat ccaggcacgg
     8221 cgcatccttg cgccttcgcg gtgtttccct gcctcgggcc agcaatgccc ggatgcttcg
     8281 ggagcgcgat gcaatcgcgt tggatcgtgt gattcctttg tgcgagcgcc aaaacgaaaa
     8341 cgcccccggg tgctggaacc cgagggcgtt cgcgtggcct cgggagatcg gggtgaccga
     8401 tctccgcgat gcgaaagatc agtcgatgtg ggtggtgccg aaataattcc gcttgccgcg
     8461 aagctgcagt gcatcgcgcc gttcgcacgc gcgcaggcct gcatcgcgct tgggcgatgg
     8521 cagcagtgca gcgatgtgga agacggaggt catgggaatt cgatggtccg gaatgtggtg
     8581 cggaaatttc gccgagggcg aaggtggcgc acgttacgcg cgctcttttc gtttggcaac
     8641 aaaattttta catcaccgac gagcggtgga attcctgcgg tcgtgcggcc ggaaaccgcg
     8701 gaaaccgggg tcagagtcga ctttcgctgg tcgatcgttc ttatcgcgag gcgatcattc
     8761 gaaagtcgac tctgaccccg gtttcccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnngat cgttcttatc gcgaggcgat cattcgaaag tcgactctga ccccggtttc
     8941 ccgatcgtcg cgcattgcga acacaaaaaa gcccgcattt ctgcgggctt tttgattcca
     9001 tccgaccgga aggcttactt gatcttgcct tccttgtaca tcacatgctt gcggacgacg
     9061 ggatcgtact tgctgacctc catcttcgcc ggcgtgttct tcttgttctt gtcggtggtg
     9121 tagaagtggc cggtgccggc cgaggaaatg aggcggatct tgtcgcgctt ggtagccatg
     9181 attcagttct cctcagacct tctcgccctg cttgcgcagg tcggccagga tggcatcgat
     9241 gccgttcttg tcgatggtgc gcagggccgc agccgaaacg cggagcttga cccaacggtt
     9301 ctccgaggcg acccagaagc ggcgctcgtg gaggttgggc atgaaacgac ggcgggtctt
     9361 gcggttggag tgcgagacgt tgttgccgga aatcgtgcgc ttgccggtta cttggcagac
     9421 tttggacact gtgcacctcg atcttgatgg gttgtcgccc gtagcccggg aaacggcggc
     9481 cccgctgcgt tcccgagggg ttcgcggcac acgaaacggg tagtttccac gctgccaacg
     9541 gcgggccgga ttcgtgttcc gggcccgccc gtcacctgcc agaaggcggt gccggacaca
     9601 gcgagccgcg cattatgcgg acaaaatcct ttgtgggcaa gcacttggct gcagaggagc
     9661 gcctggcgtg ctggaaacgg ggtcagagtg aattttgcgc cgacgctgat cggtgaatcc
     9721 acggcggatg gccgcaaaac ttcactctga ccccattggg gcgcctggct cccccggcgc
     9781 ctacttcgcc ttcgcctcgg cgatataggc ccggatctgt gactccagca ccggcagcgg
     9841 caccgagccc tgcgccagca cggcgtcgtg gaaggcgcga acgtcgaact tcgggccgag
     9901 cgcggcttcg gcctcgcggc gcaggcgcat gatcgtgagt tcgccgagct tgtagctcag
     9961 cgcctggccg ggccagctga tgtaacggtc gacctcggtg gtgacctcgt gctcggacag
    10021 cgcggtgttg tcgcgcaggt aggccagcgc ctgctcgcgg gtccagccct tgtggtgcac
    10081 gccggtgtcg atgaccaggc ggcaggcacg ccacatcgca taggtgaggc gcccgaaatc
    10141 ctcgtacggg gtttcataga tgcccatttc cttgccaagc cattccgaat acaggcccca
    10201 gccctcgcca taggcgctga tgtagtcctt gcggaagtcg ggcacgtcgc cctgttcgcg
    10261 caccagcgag ccctgcaggg cgtggcccgg cgaggactcg tgcagggtca gtgccggcag
    10321 gttgtagagc ggacgcgagg gcaggttgta ggtgttgacc cagtaggtgt ccgcaccgcc
    10381 gcgaccggcg gtccagaacg gcgcgatgtc cggcggcacc ggcacgatgg tgaagcggcc
    10441 gcgcggcagg gtgccgatga gcttgccgat ctgcccatcc acccgcttgg aaatccacgc
    10501 ggcgcgatcc agcaactcct gcggggtctt cgcgtagaac cggggatcgc tgcgcaggaa
    10561 ggcgaggaag tccgcgaagc tgcccttgaa cccgacctgc tggatgatgc cgtccatctc
    10621 cgcgcggatc gacgcgactt ccttcaggcc gatcgcgtgg atctggtcgg cggacagatc
    10681 cagcgtggtg tatttgcgga tctgttcgcg ataccaggct tcaccctccg gcattgcttc
    10741 ggccgcgagc gtcgtgcgcg ccttcggcat gtattcgctg cggaaaaaca cgagcaattt
    10801 cgcgaacgcg ggcaccacgg catcacggat cgcggcgcgg ccttctgcgc gcagggtttc
    10861 ctgctcggcg ggcgggatcg tgctcggcat cttgcgatac gggtcataga agttggagga
    10921 ctcgatgtcc ttcaactccg ccaccatcgc gatggagcca tcgcggccga ccagcaccgc
    10981 gcgcggcacg ctgaagccgc gtgccaggcc ggcgcgcatg ttcgcggtct gttggtcgaa
    11041 gtaacgtggc acgtcgcgca ggcgcgcggc ataagcgcgg taggctgcgg catcgcgcag
    11101 gtcggcgcgg gccatgaagc cgaggtcgga ccagaacgag ctgtcggcgt tgaacggcat
    11161 ctcgtagtcg cgcgcgcgca actccgccgc catgtgctcg agttgcgggc ggtagaccgc
    11221 gaagttcacc tggttcgcag gggagagcgt gctggcgtcg atgccgtcga gttgcctgag
    11281 cacgccatcg aggtaggcaa gccgcttcgc ctgcgccgcc gggccgacat cggcgagcct
    11341 ggtcgagggc gtgcgcgcgc tgttgtcgct gtcttcgtcc caactcggcg cctgctgcgt
    11401 gcgccagtcc cactcggcct tgtagatggc ttcgaacgtc ttgtcggtcg ggctcgatgc
    11461 gggcggcgtg gcggcgtgcg cggcgatcgg aagcaacagg cagcaggcga ggacgaagga
    11521 tttcacgcgg cggcatccgg catcgaggga aaaggggtca gggaaaaggg gccagagtga
    11581 attttgcgcc agtcaagtct ggcaaatccg atcaggctgg tcgcaaaact tcactctggc
    11641 cccttttcct gcatcactct gaccgtttct cctgccaggc gcctcaatgc tccgcgcggt
    11701 cccgatgcca gccgcgatgc ttgatgtcca ggtacaggct gtagcaggcg gtgaaactgg
    11761 ggaacaggtt ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgcttgat
    11881 gtccaggtac aggctgtagc aggcggtgaa actggggaac aggttctgca ggatgccgac
    11941 cgcgtcgttc ttgccgaaga tgaaatacgc cagcgtcatc aggctgccaa ggatgctcat
    12001 gtaccagaac aggcgcggga tcaccggctt gccctggcgc ttgctggcga tgaactggac
    12061 cagccagcgg ccgccgaaca tcagcgcgcc gaccagcccg atcagcttcc acgggctcat
    12121 gtggataccg gtccattcca gccacggaat ggcggaattc atcacgtcgt gcgcgaccgg
    12181 tgtcaccgcg gcagttcttc caccgcggtg cggcggctgc gcacgatcag ccaggccacg
    12241 ccgcgcaggt cgcggatccc gaccagggcg cggttgaggt tgttgtactt ggacacgccg
    12301 gcggtgcgat ggcggtggtt gaccggcacg ctcaccgtct tccagccggc acgctgcatc
    12361 agcgcgggca ggtagcgatg catgtggtcg aagtagggca ggtcgaggaa ggcctcgcgc
    12421 tcgaacagct tgatcccgca gccggtgtcg ggcgtgtcgt cgcgcaacat gcgcgcgcgg
    12481 atcgcgttcg cccacttcga cgcccagcgc ttgctgccgc tgtcctggcg attcacccgc
    12541 cagccggcga acagcttgat gtcggcggcc gcggcatcgc gttgcgcaag cagtttcggg
    12601 atgtcggcgg ggtcgttctg gccgtcgccg tccaacgtgg cgatccaggg cgcgcgcgcg
    12661 gccttgacgc cgttgcgcac cgcggtgctc tggccggcgt tgctgaggtg gcggatgacg
    12721 cgcagttccg gcgtggtcgc ctgcaggtcg cgcaggcgtt gcagcgtcgc gtcgcgcgag
    12781 gtgtcgtcga cgtagacgat ttcgaagctg atcacgccac gcaacgcagc gacgatctcg
    12841 ccgaccagcg gcgtgacgtt gtcttcctcg ttgtgcaccg gcacgacgac cgacagctgc
    12901 gggttcccac tggcgatgct cgcgctcatg cgtggcgtgc cctcaaggct tggtgcggag
    12961 gcggtccagc acgccgtcca gtacgtcgaa atcggcgtaa tggatgacca gcttgccctt
    13021 cttgccgcgc ccgggcacga cttccacgcg gctgcccagg ctttccgaca gttcgcgctc
    13081 cagggtgacg atgtcggcct gcggcctcgc cttcggcgcg gccttcttcg gcggcgcggt
    13141 gaccttgccg gatgccagct gctgcacgcg gtgttcgact tcgcgcaccg accagccgtg
    13201 ttcggcggcc tgcttcgcca gcgagatcgc catcggcgcg gccagcggca acaacgcgcg
    13261 cgcatggccc atctcgatgg aaccggcctg caccagcgca cggatctcgg ccggcaattc
    13321 cagcaggcgc agcaggttgc tcactgcggc gcgcgaacgt cccaccgctt ccgcggcggc
    13381 ggcgtgggtc aggtcgaatt cgtcgatcag gcgctgcagt gcctgcgctt cctccagcgg
    13441 gttgaggtct tcgcgctgga tgttctcgat cagcgccatc gccaccacgg tgcggtcgtc
    13501 gaccacgcgc accaccaccg gcacttcggc caggccggca cgttgcgacg cacgccagcg
    13561 gcgttcgccg gcgatgatct cgtaggtgcg accgcccttg ccgtcggcgc cgacatgctc
    13621 gcgcaccacg atcggctgga tcacgccctg cgagcggatc gacgcggcca gttcctccag
    13681 cttcgcgtcg tccatcgagc gccgcggctg gtatttgccg ggcaccatcg catcgaccgg
    13741 caaggtgcgc agcgtgtcgc cgggcatcgc ctgcaacggc ggcgcctcgg cggcggcctt
    13801 ggggccgagc aacgcttcca gtccgcgccc gaggccgcgc ttcttcgccg cgctcttcgc
    13861 gggttggttg ccggccgtca tgccgccttc tccttggatg cctgggtgcg ctcgcgctgc
    13921 cggcgcaaga gttcgccggc caggccgaga taggccacgc cgccgcggct ggccttgtcg
    13981 taaccgatga tgctctggcc gtggctgggc gcctcggcca ggcgcacgtt gcgcgggatg
    14041 atggtgcgga acaccaggtc gccgaaatgg ttggtgagct cggcggacac cgcgttggcc
    14101 aggttgttgc gcacgtcgaa catcgtgcgc aacacgcctt cgatctgcag cgcggggttg
    14161 agccgaccct tcagcgcatc gatggtgtcg atgagggcgc tcaggccttc cagcgcgtag
    14221 tactcgcact gcatcggcac cagcacggaa tccgatgcgg tgagcgcatt gagcgtgagc
    14281 agcgacagcg acggcgggca gtcgacgatc acgaagtcgt agccatcgcg caccgtgtcc
    14341 agcgcctgct tgagcttctg ctcgcggccg ggcatgtcca tcaactggat ctcggcggcg
    14401 gtcaggtcga tgttgccggg cagcaggtcg tagccgtcct ggcaacgcac gatcgcctcg
    14461 cgcgcctgcg cctcgcccag catcacgttg gtgatggagg tctcgagctc gcgtttctcg
    14521 atgccgctgc ccatggtcgc gttgccctgc ggatccaggt cgaccagcag cacgcgcttg
    14581 ggcgtgcgcg ccagcgcggc ggcaaggttc acggcggtcg tggtcttgcc gaccccgccc
    14641 ttctggttgg cgatggcgat gatgcgggcc atgcggcgtg cgtgtccttc ggggagccag
    14701 cgcaggagac gccgggcggt cgattatgcc tcagagcccg cccgcacgac ttcgaccagg
    14761 tggcgttcgc cgaccagccc gggtacccgc agtggatgcg ccgcggccag ccgccagccc
    14821 gcgggcagcg cggcgatttc ctcgctgggc aacacgccct tcatcgccag caggcggccg
    14881 tcggtgccga gcaagtggcc gcccagcgcc aggattagcg gcagggtggc cagcgcgcgc
    14941 gcggtgatgg cgtcgaaggt ggcaccgggc ttgaacgcct cgatccgcga ttccgccacg
    15001 ctggcgtttt ccagcttgag ctggcggatc gcctcacgca ggaaccgcgc cttcttgccg
    15061 ttgctctcga ccagggtcac ctgcagcgag ggcgtggcga tcgccagcgg gatcccgggc
    15121 aggccgggac cggtacccag gtcggccagc gtgcccagcc cgcgcacgaa cggctgcatc
    15181 gccagcgagt ccagcaggtg cttggcgatc atctcgcgcg ggtcgcgcac cgcggtgagg
    15241 ttgtaggtcg cgttccatcg cgcgagcaac gcgacatacg caagcagcgg actggccagc
    15301 gcaaggtcca gcgacagcgc ggcaaggccg gcttccagtt cggggcgcaa cgcggacatg
    15361 tcatcgctca tgggcgaagt atgaaggcca gctggcggcg gtgcatcgcc cgcggaatcg
    15421 cctgcgatcg cagacatccc cgtcacatcg gcgcgcggag actacgccca ccccgagcac
    15481 cccacgtcga ggggcgccgt gaagtccaac gaactttccg cgtcataccc gcatcttttt
    15541 tccaggagcc caacaccatg aacttcctca tctacctcat cgtcggcggc atcgccggtt
    15601 ggctggccag catcatgatg aagcgcgacg gctcgcaggg catcatcctc aacgtcatcg
    15661 tcggcatcat cggcggcttc ctgggcggca tcctgctgcc gatggtcggc ctgtccttcg
    15721 gcggcacgat cggcttcctg atcaccgcct tcatcggcgc ggtcgtgctg ctgctgatcg
    15781 tcaacctgtt cacccgcggt cgcgcgcgct aagcgtcgat ccgcacacgg atgtgcaagg
    15841 aaagcccggc attgccgggc tttttctttg ctacggatca atggcggcgg atcaacccgc
    15901 ggccagttgc acccacgccg gcgtgtggtc gctggcgcgt tcccaggtgc gtggcgtgcg
    15961 gtcgatgccg gcttggatcg cgtcggcctt caaggcatcg gacaccaggg tgaggtcgat
    16021 gcgcagcccc aggtcgcggc ggaacgccgc ctgccggtaa tcccaccagc tgaagatgcc
    16081 ggcatcgtcg tggtgcaggc ggaatgcatc gtgcaggccc agctcgagca ggccgcgcag
    16141 cgcgccgcgc tcggcggtgg aggtgaggat gtggtcgtcg ttccagacca tcgggtcgtg
    16201 cacgtcgcgg tcgtcgggcg cgatgttgaa atcgcccagt accaccagtt tcggatgccg
    16261 ctgcagctca tcggcgaccc atgcgtgcac cgcgtcgagc cagcgcagct tgtaggcgta
    16321 cttgtcggtg ccgatgtcct ggccgttgac cacgtacagg ttgacgatgc gcacgccatt
    16381 caccgtcgcc gcgatcgcgc gcgcctgcgc gtcgtcgaag ccggggatgc ccagttgcac
    16441 gtcgccgatc gggtgccgcg ccaggatcgc cacgccgttg taggtcttct ggccgacgaa
    16501 ggcgctgcga tagcccgccg ccagcaacgc atcctcgggg aacttgtggt cctcgagctt
    16561 ggtttcctgc aggcccacca cgtccggcgc gaactcctgc agccactgct gcaggtgcgg
    16621 caggcgcaca ttgagcgaat tgacgttcca gctggcgatc ttcatccgga cctcaacgca
    16681 cgatcaaatc aagcaaccac ttcgccttcg aatacggtgg tttgagcagg tcgccgccgg
    16741 tgcggcgcgc ctgccacagc accggcaatt gcttgctcat cgcatcgaag ccggcgcggc
    16801 cgtggtaggc gcccatgccg ctggggccca cgccgccgaa cggcaggtcg gtgatcgcga
    16861 aatggaacag ggtgtcgttg accgagaccc cgccggccag ggtgtggtgc aggatttttt
    16921 ccaccgtcgc cttgtcgtcg ctgaagggat acagcgccag cgggcggtcg tgcgcattga
    16981 tgtaggcgat cgcctcgtcg agcgtgcggt aggtcttgat cggcagcagc gggccgaaga
    17041 tttcctcctg catcaccgtg gcgtcgtcgc ccgggtccag cagcagggtg ggcggcatga
    17101 gccgttgcgc gggatcggga tgcccggcca gggtgacctt ctcgatgccg cgctgcttgg
    17161 catcgtccag gtagcccttg agccgcgcgt actggccgtc gttgatgatc cgcgcgtagt
    17221 cgccgacgtt ctcgaaatgc tcgccgtagc gcgccagcac ctccgcgcgc agcgccttca
    17281 ccagcgcgtc gcgcttgccc ttcggcacca gcacgtagtc gggggcgatg caggtctgcc
    17341 cgccgttgaa ccacttgccg gtggcgatgc gcgcggcggc gcgttcgatc gggtagtcgt
    17401 cggcgatcag cgccggcgac ttgccgccca gctccagggt gagcggcgtc aggttcggcg
    17461 ccgccgcggc catcaccttg cggccgaccg cggtggagcc ggtgaacacc aggtggtcga
    17521 acggcaggcc ggcgaaggcc gcgccgatgt cggcaccgcc ggtcgccacg acgacgcggt
    17581 cgggcgggaa cacctcggcc aacagttcgc gcaggaactg gctggtcttc ggggtgtgtt
    17641 cggacggctt caggtagacg tggttgccgg cggcgatcgc cgtggccagc ggaatcagcg
    17701 agaggttgac cgggtagttc cacggcgcga tcaccccgac cacgccgacc ggcaccgggc
    17761 gcacctgcgc gcgcgccggc aggaagcgcc agcccacgcc cacgcgcgtc ggcttcatcc
    17821 agcggcgcag gtgcctgatc aggtggtcga tgtcgttcag cacggtcatg ccgtcggcga
    17881 ccacgctttc cgcgcgcggg cgatggccga agtcggcggc gatggtggtg gccatcgcgt
    17941 cgagcttgcg cttcaggaca tcgcgcaggc gttgcaggtc ggcgtggcgc tgcgtgtaat
    18001 ccggcttgtt cgcctgccag gcttcgcgca ggcgttgcag ggtgggagcg aggtctgaaa
    18061 tcggagtgtc catgcgccga agtgtagacc ggcgccgcgg gcatggccgc tagaatgggc
    18121 catgacctcg aacacgcccg tgctgcgccc gtatcgcgac atcctgccca ccgtgggcca
    18181 gcgcgcgtat gtcgatcccg ccgcgaccgt gatcggcgac gtggtgctgg gcgacgacgt
    18241 ctcgatctgg cccggcacga tcgtccgcgg cgacgtcaac ttcatccgca tcggcgcgcg
    18301 caccaacgtg caggacggca gcgtgatcca cgtcagccac gatggcccgc acgccaagct
    18361 gggcggcttc gccaccgtga tcggcgagga cgtgaccatc ggccacaagg cgatcatcca
    18421 cgcctgccgg atcgaggacg cggtgctggt cgggatgggc gccgtcgtgc tggacggggc
    18481 ggtggtgaag aagcacggct tcgtcggcgc ggggacgttg atcgcgcccg gcaaggtcgt
    18541 cggcgagggc gaactctggc tgggcaaccc gggcaggaag gtccgcatgc tgtccgacgc
    18601 cgagatcgag gcgttgtact acagcgcgca gcactacgtg cgcctgaagg accattacct
    18661 ggcgtgaacc cgtccggtgt gccggcggcc gggttccccc gcgccggcca tccgactaga
    18721 gtcgtcacca gggcgagaca ccccgcagcg aaggaca
//
LOCUS       DAOOCX010000042        18441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2658_length_18441_cov_7.659632, whole genome shotgun sequence.
ACCESSION   DAOOCX010000042 DAOOCX010000000
VERSION     DAOOCX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 18441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18441
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2658_length_18441_cov_7.659632"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK399_05800"
     CDS             <1..263
                     /locus_tag="PK399_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPW12664.1"
                     /translation="LRAAWAKAVADRTGGRFSTEASVAKLTASEAAMFITHQALQIHG
                     GMGYSKEMPLERYFRDAKITEIYEGTSEIQRLVIARHETGLR"
     gene            340..5289
                     /locus_tag="PK399_05805"
     CDS             340..5289
                     /locus_tag="PK399_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HPW12665.1"
                     /translation="MSKKQATANPATPANHLDPILAALAGRVAKATLAEARAFATAFY
                     QRMDAEEFAQHGAEGWAELAADMLEFVRARKRGKASIQLFNPSLKAEGWESPHTVVQV
                     ANDDMPFLVDSVTMALAEMGIGVHVLGHPVIRIRRDKAGKLLAIGEGERESLMHLEID
                     RQSQAAMATIKARLAQALEDVRAIVADWQPMQDKMRAVADELGTRRMPLPDEGKREAQ
                     EFLRWAANDHFTFLGYREYTVRKQGKDEVLVADEDSGMGLLRGKDSGAPRLLKSLAAH
                     GMPQSGAVDALILTKTNARATVHRPGYMDYIGVLGFDAKGKPVSEQRFLGLYTSSAYN
                     RRPWDIPVVRQRHEYVMQQSGLDPNSHSGKALRHILETLPREELFQSSEEELFRTGMG
                     ILGLQERVRSKLFLRRDRYGRYFSALAYIPRDRMSTQVRHKIEAMLKRALDGEQVDTN
                     VKIGESPLAQLHIIVRPKGSEQVDVDLPALEAKLASIVRDWRDELREQLVKRHGEEAG
                     LKLADCYGRALPAGYIEEVSPGIAATDVEHLAALAGPDDLRLSLYRSRRKDGGLRFKL
                     YRQLDDIPLSDALPMMENMGLRVISEHPYKVDAASGLAYIQDFEVESLQGELDIDAID
                     EDFEDAFARVWRGDAENDGFNRLILTAGLSWRQVAMLRGYCKYLLQVGVPFSQSYMEA
                     TLARYPLLARLLVELFEARFDPRTGSESDAEIKRGMSRFGAQLKSLSADDEAAMTALK
                     SVVEARAKGRDAQVDAARNALLGLMDRVSSLDEDRILRSFIGVIDATLRTSYYIDYKG
                     GLRKDGGPADYVSYKLDSSKVPDLPKPRPYREIWVCGPRVEGIHLRFGPVARGGLRWS
                     DRREDFRTEVLGLVKAQMVKNTVIVPVGSKGGFYCKQSPDASVNRDAWFAEGVACYKR
                     FINGLLDITDNLAADGKVLPPAGVVRHDGDDPYLVVAADKGTATFSDTANGIAQAHGF
                     WLDDAFASGGSVGYDHKGMGITARGAWESVKRHFRALGRDCQQEDFTCVGIGDMSGDV
                     FGNGMLLSKHIRLVAAFDHRHIFIDPNPDAAKSFKERARLFKLPRSSWEDYDKALIGK
                     GGGVFPRSAKSIPLSAEAKAALGIESDATAMSPNELMNAILRAPVDLLWNGGIGTYVK
                     SSRENNADVGDRANNGLRVDGNQLRCRMVGEGGNLGLTQLGRIEAALHGVLLNTDFID
                     NSAGVDTSDHEVNIKILLNAEVQKKKLKLADRNTLLASMTDEVAQLVLNDNYRQNQAI
                     SLMERMSVSRLGSKQHFIRTLESQGLLDRQIEFLPSDAELAERKARGQGLTRPELSVL
                     LSYSKLVAFQQLLDSDVPEDAYLSKELERYFPQPLQAKYAKTMQQHRLKREIIATAVT
                     NSTINRMGATFLLRMQEDSGRSPGEVAKAFTITRETLDARELWAQVDALDGKVAESVQ
                     IDALQVIWEIQRSFTRWLLSRPGAIPDITSAVARFHDGFRDIRAGEGILPASQRPAYE
                     ASRKAWRDGGVPTALADQLAALPYLEASPNIIELARERKLRAVEVAKVYFRLGDALRE
                     PWLQAQIDALKVDGRWHAVARGVLRDELSTQMRTLTDQALAMPGKDAEAKVAAWMARD
                     DQTLRFTLAMLNELAAQKSLDYPTASVAVQRLAQLASRG"
     gene            5420..6187
                     /locus_tag="PK399_05810"
     CDS             5420..6187
                     /locus_tag="PK399_05810"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HPW12666.1"
                     /translation="MSTLRLALLASPADEAQAALAALTTLHPQHAPQDADVIVALGGD
                     GFMLQTLHRHGALGKPVFGMKLGTVGFLMNQYRPEELRERIASAEPAVLHPLEMLAQT
                     ESGTTVGSLAYNEVSLLRQTRQAAHVSIELNGDVRLDELICDGVMLATPAGSTAYNFS
                     ANGPILPLGANVMALTPIAPFRPRRWRGAVLKSGTEVRFRVLDPHKRPVSATADSHEV
                     RDVIEVLVREQRERSVTLLFDPEHNLEERIMIEQFTV"
     gene            6314..7225
                     /locus_tag="PK399_05815"
     CDS             6314..7225
                     /locus_tag="PK399_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006450285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HPW12667.1"
                     /translation="MPEPVAILTVAITARALFHMEDSHGLFEAEGVKAYAEFQRERED
                     DVLEPGIAFPLVRKLLALNDGTPADAPRVEVILLSRNSSDTGLRVFNSIQHYGLDIRR
                     ATFTSGAPVWPYIVPFGAQLFLSANPDSVRSALEAGVAAATILPARAPASQHDQLRIA
                     FDGDAVIFGDESERVSREQGVEAFGRHERERAREPLSGGPFRGFLSALHALQAAFPRG
                     ESSPIRTALVTARSAPAHERVIRTLREWEVRLDEALFLGGRGKGPFLQAFGADIFFDD
                     SPHNIDSARLHVAAGHVPHGVANEPAR"
     gene            complement(7233..7775)
                     /locus_tag="PK399_05820"
     CDS             complement(7233..7775)
                     /locus_tag="PK399_05820"
                     /inference="COORDINATES: protein motif:HMM:NF022607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HPW12668.1"
                     /translation="MKKKTKWLLAIAGLILFALLAFVVAGPWLAINGIRNVVASGNYG
                     ELWRFVDFDQLRENVRPQIQARIARGIIERAGAGSTTDAIAGVTDLISKPAIDAMVSP
                     LGVATLLRGSALVRQVSGQKDADGKARAADPLKDAKTGYVSPSLFTATVQNAEGQPVV
                     FQFRRSGLAWKLTGIDLPED"
     gene            complement(7858..9039)
                     /locus_tag="PK399_05825"
     CDS             complement(7858..9039)
                     /locus_tag="PK399_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4785 domain-containing protein"
                     /protein_id="HPW12669.1"
                     /translation="MRTSIHRVAALAASATLFVAGIAHAAQPLLPAGASDQVPTRLSV
                     LPAPTGAIERKPVAFSWALDPDAELSDTAPFLAESREYWMTVEGSELARGVPLQMTAP
                     GALIRVSPARGSLGLRAGDFSVSMAGRKVGIARAADAETLRKAGMDASTGTQVLRLGD
                     GSGAGRHVLRAANARGRYVVHVFEPASDRVLFARADRNHALAGTPMRVSIGATDAGRK
                     APVASQALLVAPDGSSQSVAVHDAVGGGQEAVFTVPATASQAKGLWELQVFGDVGDVP
                     RDARTAFAVAQPTARFSGIFGVDAANLRVSLPVEAASPGRYEARGTLYATASDGSLRP
                     VSQAHAAAWMDAGDGMLVLQFARAHVPAGYGAPFEVRQLELNDQGRMAPIESRTRGAR
                     F"
     gene            complement(9051..9923)
                     /locus_tag="PK399_05830"
     CDS             complement(9051..9923)
                     /locus_tag="PK399_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12670.1"
                     /translation="MRKHAFGAVLSCAAVLTASPAIAADVTGVAFVHGTGSQTNAYND
                     YWQPTMVNNVRNGLANPANYVVINCDFNQYMWDSRAAGCLAGQLTTFINAKGITRLIV
                     ITHSNGGNVVRWIMSNPTYDSRYPNIINKIIRVNALAPSSAGTPLADATINGNVFETS
                     LGWLLGYKSDAVRMQQVSWMASYNANNLYGTSGRPALPKLFRSVVGTDVDSAIWDADS
                     YCAGYVENVGLETTQNWLNSCSDGFLNCSSQSAAGSVWFQDKTRMAGSEPLSHNQSRR
                     ACFGLDTILRNDMK"
     gene            complement(10066..11511)
                     /gene="sbcB"
                     /locus_tag="PK399_05835"
     CDS             complement(10066..11511)
                     /gene="sbcB"
                     /locus_tag="PK399_05835"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010366550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HPW12671.1"
                     /translation="MTASFLFYDLETFGADPRRTRIAQFAAIRTDAALDQVDEPISFF
                     VQPANDLLPSPIATMITGIAPQDALRDGVNEAEACARIVEEMARPETCSLGYNSLRFD
                     DEFVRHGLYRNFFDPYEREWRGGNSRWDLLDALRLMHALRPDGIAWRPREDGGGTSFK
                     LEHLAEDNGLRIGDAHEALSDVRATIGLARLFRQAQPRLWDYALRLRDKRFAGSLLDV
                     VAMTPVLHVSQRFPASRLCAAPVIPLARHPQIDSQVVVFDLAEDPDALLDLDAEAIAE
                     RLYIRQADLPEGERRVPLKTVHLNKSPALVAWDHLRDADFARLGIDRDLVQARASRIR
                     DTGPALAEKVRRVFARDRPAEDIDVDAALYGGFIGDGDKRLLAQVRGTPPHLLAKAQY
                     AFRDSRLPELLFRYRARNWPDSLLPAERSRWDDYRRLRLQADNGLSETTFGDFNAEIA
                     QLRIAHAGDGRRLALLDRLQAWGDGIAQTLE"
     gene            complement(11628..12980)
                     /locus_tag="PK399_05840"
     CDS             complement(11628..12980)
                     /locus_tag="PK399_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006450280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW12672.1"
                     /translation="MTADQREITIVGGGLAGALLAILLAQRGWSVDVYERRGDPRVHG
                     YAGGRSINLALAERGLHALRQAGADAAVMKQAVMMRGRYVHPLHGEPGLQRYGRDDSE
                     VIWSINRGELNIVLLDIAESHGARLHFEHGLASVDFVDKVAVFKGDGHETRRPFRALV
                     GADGAGSSLRAEINKVLPLGERTEWLDHGYKELEIPPAKDGGFRIEPNALHIWPRGHY
                     MCIALPNDERTFTVTLFMPHAGAHPSFETVRDADEAQAFFERDFPDTLPLIPELRHDY
                     EANPVGILGTLYLDHWHLHGHAVLIGDAAHAMVPFHGQGMNCAFEDCVALADHLDANA
                     SLSDAFAAFEAQRKPDAEAIQQMALENYIEMRDKVDDASFLLQRQVELALQDRHPGRF
                     VPHYAMVSFMRIPYSVALHRSEVQRVILEDATRGLASLHEVDWDAADAAVLERLDPIA
                     "
     gene            complement(12970..14244)
                     /gene="kynU"
                     /locus_tag="PK399_05845"
     CDS             complement(12970..14244)
                     /gene="kynU"
                     /locus_tag="PK399_05845"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HPW12673.1"
                     /translation="MTEIFSDAYAASRDAFDPLGDFRGEFHLPLHDGKPQAYFVGNSL
                     GLQPKGARAHVEEVLDKWATQAVEGHFTGQAQWMPYHELVRDPLARVVGAQPREVVAM
                     NSLTANLHLMLVTFYRPTRERPVLLMEAGAFPSDRYALESQLRFHGFDPAEALVEVVP
                     DEADGTFSMAAIERAIAEHGSRLACIVWPGVQYRTGQAFDLEEIARLGHAAGAIVGFD
                     LAHAVGNLPLQLHDSGADFAVWCHYKYMNSGPGAVAGCFVHERHARTNRPRFAGWWGN
                     DPGVRFRMGPEFNPTPGADGWQLSNPPILGLAPLRASLDQYDRATMPALRTKSESLTG
                     YLEQLIDAGLRDVLQVVTPREAARRGCQLSIRVIGGRERGRDLFEFLASRGVLGDWRE
                     PDVIRISPVPLYNTHADVLRFANTVREWRDDR"
     gene            complement(14241..>15149)
                     /locus_tag="PK399_05850"
     CDS             complement(14241..>15149)
                     /locus_tag="PK399_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW12674.1"
                     /translation="IYKDGKFFREIWPKTWDPQIRIDDYAGFGVQVQVLSTVPVMFSE
                     WAKPHQALELHKSLNEHMARTCRDYPRHYAGIGTVPLQSPQLAIRELERCMDELGLQG
                     VQIGSHLNGPGDAHWNLDAPELFDFFQAASDLGAAILVHPWDMMGTDTMPKYWLPWLV
                     GMPAEQSRAACCLVFGGVLERLPRLRVCMAHGGGSFPYTIGRIEHGFNMRPDLVATDN
                     FRNPREYLKRLYFDSWVADDAALRYLLEACGIDRVMLGTDYPFPLGEQVPGEGIERLA
                     LDEAQRARIYHGTALEWLGLPASRFA"
     assembly_gap    15150..15249
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15250..15794)
                     /locus_tag="PK399_05855"
     CDS             complement(<15250..15794)
                     /locus_tag="PK399_05855"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HPW12675.1"
                     /translation="MLPNPINLQGWIDEHRHLLKPPVGNKCIVDADYIVMIVGGPNAR
                     TDYHYEDGPEWFYQLEGEMVLRIQEDGSARDIPIRAGEMFYLPPHVPHSPQRMPGSIG
                     LVIERKRLAHEDDALMWFCIACNHTLYEEFFHLVDIEQDFFRVFERFYRDDALRTCKA
                     CGTLNPRPTRYEMDAQSQADIT"
     gene            complement(15796..16236)
                     /locus_tag="PK399_05860"
     CDS             complement(15796..16236)
                     /locus_tag="PK399_05860"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPW12676.1"
                     /translation="MSGPDQHRSIHADAAPKAVGSYPHARRVGNTLYLSGIGPRNPVD
                     NTIPGNDYFADGRLRKYDIEAQARAVFANVRAVLDASGATWGDLVDVTVYLTDMARDF
                     KTYNALWAEHFPDAATAPCRTTLGITALPTPIAIELKCIAVLKD"
     gene            complement(16236..16898)
                     /gene="can"
                     /locus_tag="PK399_05865"
     CDS             complement(16236..16898)
                     /gene="can"
                     /locus_tag="PK399_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008576307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HPW12677.1"
                     /translation="MNRLDDLLRNNQAWAERVSREDPGFFERLSKQQAPKYLWIGCSD
                     SRVPANQVVDLAPGEVFVHRNIANVVVHTDLNCLSVIQFAVDVLKVEHILVVGHYGCG
                     GVHAALTNARVGLADNWIRHVGDVSTKHAQLLLDAGDEPLQHDRLCELNALEQVANVC
                     QTTIVRDAWVRGQTLSVHGWVYTLRDGRVHDLGLNVAASDQLEPQYAAALAAISVDKE
                     DR"
     gene            complement(16895..18196)
                     /locus_tag="PK399_05870"
     CDS             complement(16895..18196)
                     /locus_tag="PK399_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12678.1"
                     /translation="MNKRAVSIALLSLALAANAVSAGGDGTKNPVDDFFKAFNAQPDT
                     FAEYRFLTSPAVQQDAQTRNMASQMLATHLSFLGRPMDALRAFPYRGNSAPESDLPLP
                     SDWRALPASEWISAQAGDYRVVMLNEAHHAPHTRVLTLALLQPLREQGYTHLAVEALV
                     NDGTDPMPSGYPVRKTGIYTRDPVFAELLREALRLGYRLVPYETPNKPGERQQDRETG
                     QAQAIADLLAREPSAKVLVHAGYAHIGEAQQGLPDDARPMAMELARLTGLPVLTIDQT
                     STRSYETADIDTVGQRLARQFALSAPSVLLSRDGAGPWSYRPGLNDVSVLLPAAPAGQ
                     AQRPDWLSLGGRRGVVAIDLTPCAGHLPCLAEARHADEGDEAIPADQFLMLATSEAST
                     PLYLAPGKYRLRLAGADGGTLLDRPLQVPSPDANLPFAKTP"
     gene            complement(18193..>18441)
                     /locus_tag="PK399_05875"
     CDS             complement(18193..>18441)
                     /locus_tag="PK399_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW12679.1"
                     /translation="ALALANASDYGLSASVFTNDLSRAHRMAAQLRVGMVWINTWLMR
                     DLRTPFGGSGASGMGREGGLEAMRFFTEPRNVGIALPP"
BASE COUNT         3002 a         6026 c         6336 g         2977 t
ORIGIN      
        1 ccctgcgcgc tgcatgggcg aaggccgtgg ccgacaggac cggtggccgc ttcagtacgg
       61 aagcatcggt ggccaagctc accgcctccg aggcagcgat gttcatcacc caccaggccc
      121 tgcagatcca cggtggcatg ggctattcga aggaaatgcc gctggaacgg tatttccgcg
      181 acgccaagat taccgagatc tacgagggca ccagcgagat ccagcgcctg gtgatcgccc
      241 gccacgaaac cggcctgcgc tgacgcccgg ccttccatcc cgacatcgat gttccggccg
      301 cgccttgcgc ggccttctgc ctttggagcg atcgcgacca tgagcaagaa acaagccacc
      361 gccaacccgg cgacacccgc caaccacctc gatccgatcc tggctgcgct ggccgggcgg
      421 gtggcgaagg cgacgttggc cgaagcgcgt gccttcgcca ctgccttcta ccagcggatg
      481 gatgccgagg agttcgccca gcacggtgcc gaaggctggg ccgaactggc cgcggacatg
      541 ctggaattcg tgcgcgcgcg caagcgcggc aaggccagca tccagttgtt caacccgagc
      601 ctgaaggccg aaggctggga gtcgccgcac acggtggtgc aggtcgccaa cgacgacatg
      661 ccgttcctgg tcgattcggt gaccatggcg ctggccgaaa tgggcatcgg cgtgcacgtg
      721 ctgggccatc cggtgatccg catccgccgc gacaaggccg gcaagctgct ggcgatcggc
      781 gagggcgagc gcgaatcgct gatgcacctg gagatcgatc gccagtcgca ggccgcgatg
      841 gccacgatca aggcgcgcct ggcgcaggcg ctggaggacg tgcgtgcgat cgtcgccgac
      901 tggcagccga tgcaggacaa gatgcgcgcg gtcgccgacg aactcggtac gcgacgcatg
      961 ccgttgccgg acgaaggcaa gcgtgaggcg caggaattcc tgcgctgggc cgccaacgac
     1021 catttcacct tcctcggcta ccgcgaatac acggtgcgca agcagggcaa ggacgaggtg
     1081 ctggtggccg atgaagacag cggcatgggc ctgctgcgcg gcaaggacag cggcgcgccg
     1141 cgcctgctga aatcgctggc cgcccacggc atgccgcaga gcggcgccgt cgatgcgctg
     1201 atcctgacca agaccaatgc gcgcgcgacc gtgcatcgcc cgggctacat ggattacatc
     1261 ggcgtgctcg gcttcgacgc gaagggcaag ccggtcagcg aacaacgttt cctcggcctg
     1321 tacacctcca gcgcctacaa ccgtcgcccc tgggacatcc cggtcgtgcg ccagcgccac
     1381 gaatacgtga tgcagcagtc cggcctggat ccgaacagcc atagcggcaa ggcgctgcgc
     1441 cacatcctgg aaaccctgcc gcgcgaagag ctgttccagt ccagcgagga agaactgttc
     1501 cgtaccggca tgggcatcct gggcctgcag gagcgcgtgc gcagcaagct gttcctgcgc
     1561 cgcgatcgct acggccgcta tttctccgcg ctggcctaca tcccgcgcga ccggatgagc
     1621 acccaggtgc gccacaagat cgaggcgatg ctcaagcgcg cgctcgatgg cgagcaggtc
     1681 gacaccaacg tcaagatcgg cgagtcgccg ctggcgcagt tgcacatcat cgtgcgtccg
     1741 aagggcagcg agcaggtcga tgtcgacctg cccgcgctgg aagcgaagct ggcctcgatc
     1801 gtgcgcgact ggcgcgacga actgcgcgag caactggtca agcggcatgg cgaggaagcc
     1861 ggcctgaagc tggccgactg ctacggtcgc gccttgccgg cgggctacat cgaggaagtc
     1921 agtcccggca tcgccgccac cgacgtggaa cacctggcgg cgctggccgg accggacgac
     1981 ctgcgcctgt cgctgtatcg ctcccgccgc aaggacggcg gcctgcgctt caagctgtat
     2041 cgccagctgg acgacatccc gttgtcggat gcgttgccga tgatggagaa catgggcctg
     2101 agggtgatct ccgagcatcc gtacaaggtc gatgccgcca gcggcctggc ctacatccag
     2161 gacttcgagg tcgaatcgct gcagggcgag ctggacatcg atgcgatcga cgaggacttc
     2221 gaggatgcgt tcgcgcgcgt gtggcgcggc gacgccgaga acgacggctt caaccgcctc
     2281 atcctcaccg ccggcctgtc gtggcggcag gtggcgatgc tgcgcggcta ctgcaagtac
     2341 ctgctgcagg tcggcgtgcc gttctcgcag agctacatgg aagccacgct ggcgcgctac
     2401 ccgctgctgg cgcgcctgct ggtggaattg ttcgaggcgc gcttcgaccc gcgcaccggc
     2461 agcgagagcg atgccgagat caaacgcggc atgagccggt tcggtgcgca gctgaaatcg
     2521 ctgtccgccg atgacgaggc cgcgatgacc gcgctgaagt cggtggtcga ggcccgcgcc
     2581 aagggccgcg atgcgcaggt ggacgccgcg cgcaatgcgc tgctgggcct gatggatcgc
     2641 gtgtccagcc tcgacgaaga ccgcatcctg cgcagcttca tcggggtcat cgacgcgacc
     2701 ctgcgcacta gctattacat cgactacaag ggcggcctgc gcaaggacgg cggcccggcc
     2761 gactacgtca gctacaagct ggattcgtcg aaggtcccgg acctgccgaa gccgcgcccg
     2821 taccgcgaga tctgggtctg cggcccgcgc gtggagggca tccacctgcg cttcggcccg
     2881 gtcgcgcgtg gcggcctgcg ctggtccgac cgccgcgagg atttccgcac cgaagtgctg
     2941 ggcctggtca aggcacagat ggtgaagaac acggtgatcg tgccggtcgg cagcaagggc
     3001 ggcttctact gcaagcagtc gccggatgcc tcggtgaacc gcgatgcgtg gttcgccgag
     3061 ggcgtggcct gctacaagcg cttcatcaac ggcttgctgg atatcaccga caacctggcc
     3121 gccgatggca aggtgctgcc gccggccggc gtggtccgcc acgacggtga cgatccctac
     3181 ctggtggtcg ccgccgacaa gggtaccgcc acgttctccg ataccgccaa tggcatcgcc
     3241 caggcgcacg gcttctggct ggacgatgca ttcgcgtcgg gtggttccgt cggttacgac
     3301 cacaagggca tgggcatcac cgcgcgcggc gcctgggaat cggtcaagcg ccacttccgt
     3361 gccttgggcc gcgactgcca gcaggaagac ttcacctgcg tgggcatcgg cgacatgtcg
     3421 ggcgacgtgt tcggcaacgg catgctgctg agcaagcaca tccgcctggt cgccgcgttc
     3481 gaccatcgcc acatcttcat cgacccgaac cccgatgccg cgaagagctt caaggaacgc
     3541 gcgcgcctgt tcaagctgcc gcgctcgagc tgggaagact acgacaaggc gctgatcggc
     3601 aagggcggcg gcgtgttccc gcgctccgcc aagtcgatcc cgttgtcggc cgaggccaag
     3661 gccgcgctcg gcatcgagtc cgacgccacg gcgatgagcc cgaacgaact gatgaacgcg
     3721 atcctgcgcg cgccggtgga cctgctgtgg aacggcggca tcggcaccta cgtgaagtcc
     3781 agccgcgaga acaacgccga cgtcggcgac cgcgccaaca acggcctgcg cgtcgatggc
     3841 aaccagctgc gctgcaggat ggtgggcgag ggcggcaacc tggggctcac ccaactgggc
     3901 cgcatcgagg ccgccttgca tggcgtgctg ctcaataccg acttcatcga caactccgcc
     3961 ggcgtggaca catcggacca cgaggtgaac atcaagatcc tgctcaacgc cgaggtgcag
     4021 aagaagaagc tgaagctggc tgatcgcaac accctgctgg cgtcgatgac cgacgaggtc
     4081 gcgcaactgg tgctcaacga caactaccgc cagaaccagg cgatcagcct gatggagcgg
     4141 atgagcgtgt cgcggctggg ttccaagcag cacttcatcc gcaccctgga atcgcagggc
     4201 ctgctggacc ggcagatcga gttcctgccg agcgatgccg aactggccga gcgcaaggcg
     4261 cgtggccagg gcctgacccg gccggaactg tcggtgctgc tgtcgtattc caagctggtc
     4321 gcgttccagc agttgctgga ctcagacgtg cccgaggacg cgtacctgtc caaggaactg
     4381 gagcgttact tcccgcagcc gctgcaggcc aagtacgcca agaccatgca gcagcatcgg
     4441 ctgaagcgcg agatcatcgc caccgcggtc accaattcca cgatcaaccg gatgggcgcc
     4501 accttcctgc tgcgcatgca ggaagacagc ggccgctcgc cgggcgaagt ggccaaggcc
     4561 tttaccatca cccgcgaaac cctggatgcg cgcgaactgt gggcgcaggt cgacgcgctg
     4621 gacggcaagg tcgccgagtc ggtgcagatc gacgccctgc aggtgatctg ggaaatccag
     4681 cgcagcttca cccgttggct gctgtcgcgc cccggtgcga tccccgacat caccagcgcg
     4741 gtcgcgcggt tccacgacgg tttccgcgac atccgcgcgg gcgagggcat cctgccggcg
     4801 tcgcagcgcc cggcgtacga ggccagccgc aaggcctggc gcgatggcgg cgtgccgacg
     4861 gcgcttgccg accagctggc ggcgctgccc tacctggaag ccagtccgaa catcatcgag
     4921 ctggcgcgcg agcgcaagct gcgcgcggtg gaagtggcca aggtgtactt ccgcctgggc
     4981 gatgccctgc gcgagccgtg gctgcaggcc cagatcgacg cattgaaggt cgatggccgc
     5041 tggcatgcgg tggcacgtgg cgtcttgcgc gacgaattgt ccacgcagat gcgcacgctg
     5101 accgaccagg cgctggcgat gccgggcaag gatgccgagg ccaaggtcgc cgcgtggatg
     5161 gcccgcgacg accagacctt gcgcttcacc ctggccatgc tcaacgaact ggccgcgcag
     5221 aagtcgctgg actatccgac ggcatcggtc gcggtgcagc gcctggcgca gttggccagt
     5281 cgcggttgat gctgtttcct tctcccgctg gcgggagaag gtggcccgca gggccggatg
     5341 cgggtggttg cgtgcatcca ccctcacccc aaccctctcc cgccagcggg agagggagca
     5401 agcgcgttaa gctcgaccga tgagcacgct tcgactcgcc ttgcttgcca gccccgccga
     5461 cgaggcgcag gccgcgctgg cggccttgac cacgttgcac ccgcaacacg cgccgcagga
     5521 tgcggacgtc atcgtcgccc tgggcggcga tggcttcatg ctgcagacgc tgcatcggca
     5581 cggtgcgctg ggcaagccgg tgttcggcat gaagctgggc acggtcgggt tcctgatgaa
     5641 ccagtaccgg cccgaggaac tgcgcgagcg catcgccagc gcggaacccg cggtgctgca
     5701 tccgcttgag atgctcgctc aaactgagtc cggcacgacg gtgggatcgc tggcctacaa
     5761 cgaagtctcg ctgttgcgac agacccggca ggccgcgcat gtctcgatcg aactcaacgg
     5821 cgacgttcgc ctggacgagc tgatctgcga tggggtgatg ctggccacgc cggccggcag
     5881 caccgcgtac aacttttccg ccaatgggcc gatcctgcca ctcggcgcga acgtgatggc
     5941 gctgaccccg atcgcgccgt tccgcccgcg tcgctggcgc ggcgcggtac tgaagtccgg
     6001 caccgaagtg cgatttcgcg tgctggatcc gcacaaacgc ccggtcagcg ccaccgccga
     6061 ttcgcacgag gtccgcgacg tcatcgaggt gctggtgcgg gaacagcggg agcgcagcgt
     6121 gaccctgctg ttcgaccccg agcacaacct cgaggaacgg atcatgatcg agcagttcac
     6181 ggtctgacct tccgcaggtg tagcccggat agggccacag gccgcatccg ggacgcgatc
     6241 catcccgggt gcgcttggcg acatgggcct gcccatcgca gtccgcgaga cgaccatcgg
     6301 ccatactgcc ggcatgcctg aacccgtcgc catcctgacc gtcgcgatca ccgcgcgggc
     6361 gctgttccac atggaggaca gccatggcct gttcgaggcc gagggcgtca aggcctacgc
     6421 cgaattccag cgcgagcgcg aggacgacgt gctggaaccg ggcatcgcgt tcccgctggt
     6481 gcgcaagctg ctggcgctga acgacgggac gcctgccgat gcgccccgag tcgaggtcat
     6541 cctgctgtcg cgcaattcgt cggacaccgg cctgcgcgtg ttcaattcga tccagcacta
     6601 cgggctcgac atccgtcgcg ccaccttcac ttcgggcgcg ccggtgtggc cctatatcgt
     6661 gccgttcggc gcgcagttgt tcctgtcggc caacccggac tcggtgcgca gtgcgctgga
     6721 ggccggcgtg gccgcggcca cgatcctgcc ggcgcgagcg ccggcatccc agcacgacca
     6781 gttgcgcatc gccttcgacg gcgatgcggt gatcttcggc gacgagagcg agcgggtctc
     6841 gcgcgaacag ggcgtggagg cgtttggacg ccacgagcgc gaacgcgcgc gcgagccgtt
     6901 gtcgggcgga cccttccgcg gcttcctgtc ggcactgcac gccctgcagg cggcatttcc
     6961 gcgtggcgag tcgtcgccga tccgcaccgc gctggtcacc gcgcgttcgg ccccggcgca
     7021 cgaacgggtg atccgcaccc tgcgcgaatg ggaggtgcgc ctggacgagg cgttgttcct
     7081 gggagggcgc ggcaagggcc cgttcctgca ggccttcggc gccgacatct tcttcgacga
     7141 ctcgccgcac aacatcgatt ccgcgcgact gcatgtcgcc gccggccatg tgccgcatgg
     7201 cgtggccaac gagcccgcgc ggtaggggtc ggtcaatcct cgggcaggtc gatcccggtg
     7261 agcttccagg ccaggccgct gcggcggaat tgaaatacca ccggctggcc ctcggcgttc
     7321 tggaccgtgg cggtgaacag cgacggcgag acgtaaccgg tcttcgcatc cttcaacgga
     7381 tcggccgcgc gcgccttgcc atcggcatcc ttctggccgc tgacctgcct gaccagggcg
     7441 ctgccgcgca acagcgtggc gacgccgagc ggggagacca tcgcgtcgat cgccggcttc
     7501 gagatcaggt cggtcacccc cgcgatggca tccgtggtgc tgcccgcgcc ggcgcgttcg
     7561 atgatgcccc gggcgatgcg ggcctggatc tgcgggcgca cgttttcacg caactggtcg
     7621 aaatcgacga agcgccacag ctcgccatag tttccactgg ccaccacgtt gcggatcccg
     7681 ttgatcgcca gccatggacc tgcgaccacg aaggccagca gggcgaacag gatcaggccc
     7741 gcaatggcga gcaaccactt ggtcttcttc ttcatcggcg gatccgctgc ggtctgcctg
     7801 catcctgcgc aaacgacgac ggccgggcaa gcccggccgt cgcgtgcggt acagcgatca
     7861 gaagcgcgcg ccgcgcgtgc gcgactcgat cggggccatc ctgccctggt cgttgagttc
     7921 cagctggcgc acttcgaacg gcgcaccgta accggccggc acatgcgcac gggcgaactg
     7981 caggaccagc atgccgtcgc cggcgtccat ccacgcagcc gcgtgcgcct gcgagaccgg
     8041 ccgcaggctg ccgtcgcttg ccgtggcgta cagcgtgccc cgcgcctcgt agcgtcccgg
     8101 ggacgcggct tccaccggca acgacacccg caggttcgcg gcatccaccc cgaagatgcc
     8161 gctgaagcgc gcggtgggct gcgcgaccgc gaaggccgtg cgggcatcgc gcggcacgtc
     8221 accgacatcg ccgaacacct gcaactccca cagccccttg gcctggctgg cggtcgcggg
     8281 cacggtgaag acagcttcct ggccgccgcc cacggcatcg tgcaccgcca ccgattggct
     8341 gctgccatcg ggcgcgacca gcagggcctg cgaggccacg ggggccttgc gtcccgcgtc
     8401 ggtggcgccg atggacacgc gcatcggcgt gccggcgagc gcatggttgc ggtcggcgcg
     8461 ggcgaacagc acgcggtcgc tggcgggctc gaacacatgc acgacgtagc ggccgcgcgc
     8521 gttcgcggcg cgcagcacat gccggcccgc gccgctgcca tcgcccaggc ggaggacctg
     8581 ggtgccggtg ctggcgtcca tgccggcctt gcgcagggtc tcggcgtcgg cggcccgggc
     8641 gatgccgacc ttgcgtcccg ccatgctgac gctgaagtcg ccggcgcgca ggccaaggct
     8701 gcccctggcc gggctcacgc ggatcaatgc gcccggcgcg gtcatctgca agggcacgcc
     8761 gcgcgccagt tcgctgccct cgaccgtcat ccagtactcg cggctttcgg cgaggaacgg
     8821 ggcggtgtcc gacagttcgg catcggggtc gagcgcccac gagaacgcga ccggcttgcg
     8881 ttcgatggcg ccggtcggcg cgggcagcac ggaaagtcgg gtaggcacct ggtcggacgc
     8941 gccggcaggc agcagcggtt gtgcggcgtg tgcgatgccg gcaacgaaga gggtggcgga
     9001 ggcggccaac gccgcgacac ggtggatcga tgtgcgcatg gtcgtggccc tcacttcatg
     9061 tcgttgcgga ggatggtgtc caggccgaag caggcgcgcc ggctctggtt gtggctcagc
     9121 ggctcgctgc cggccatccg cgtcttgtcc tggaaccaca cgctgccggc ggccgactgg
     9181 ctgctgcagt tgaggaagcc atcggaacag ctgttgagcc agttctgggt ggtttccagg
     9241 cccacgttct ccacgtagcc ggcgcagtag ctgtcggcat cccagatcgc ggaatcgacg
     9301 tcggtgccga ccacgctgcg gaacagtttc ggcaacgccg gccgaccgct ggtgccgtag
     9361 aggttgttgg cgttgtagct cgccatccag ctgacctgct gcatgcgcac ggcgtcggac
     9421 ttgtagccga gcagccagcc cagcgaggtt tcgaagacgt tgccgttgat cgtggcgtcg
     9481 gccaacgggg tgccggcgga cgagggcgcg agcgcgttga cccggatgat cttgttgatg
     9541 atgttggggt agcggctgtc gtaggtgggg ttggacatga tccagcggac cacgttgccg
     9601 ccgttggaat gggtgatcac gatcagccgg gtgattccct tggcgttgat gaaggtggtc
     9661 agttgcccgg ccaggcagcc ggcggcgcgg ctgtcccaca tgtactggtt gaagtcgcag
     9721 ttgatgacca cgtagttggc cgggttggcc agcccgttgc ggacgttgtt gaccatcgtc
     9781 ggctgccagt aatcgttgta ggcattggtc tggctgccgg tgccgtggac gaaggccacg
     9841 ccggtgacgt ccgccgcgat ggcgggtgaa gcggttagaa cggcggcgca ggacaacacc
     9901 gcgccgaatg cgtgcttgcg catggactct ccccagtcag tagccgcgtc acccgcggcg
     9961 ggtgcgatgc atgcgccccc acgcctgcat cgcggtcgag catacggatc agtatgggat
    10021 tgtcatgacg cactgcgaca gcggtcacgt tttggcagga gtggcctatt ccagcgtctg
    10081 cgcgatgccg tcgccccagg cctgaaggcg atcgagcaag gcgagcctgc gtccgtcacc
    10141 tgcgtgggcg atgcgcaatt gcgcgatctc ggcgttgaag tccccgaagg ttgtctccga
    10201 cagcccgttg tcggcctgca ggcgcaggcg acggtagtcg tcccagcgcg agcgctcggc
    10261 aggcagcagg ctgtcgggcc agttgcgcgc gcgatagcgg aacagcaact ccggcaggcg
    10321 actgtcgcgg aaggcgtact gcgccttggc cagcaaatgc ggcggggtgc cgcgcacctg
    10381 tgccagcagg cgtttgtcgc cgtcgccgat gaagccgccg tacagcgcgg catccacgtc
    10441 gatgtcctcg gcgggtcggt cacgcgcgaa cacgcgacgc actttttcgg caagcgccgg
    10501 gccggtgtcc cggatgcgcg acgcacgcgc ctgcacgagg tcccgatcga tccccagccg
    10561 cgcgaaatcg gcatcacgca ggtggtccca ggcgaccagc gccggcgatt tgttgaggtg
    10621 cacggtcttc agcggcacgc gtcgttcgcc ttccggcagg tccgcctggc ggatgtacag
    10681 gcgttccgcg atggcctcgg catcgaggtc gagcagggca tccggatcct ccgccaggtc
    10741 gaacacgacc acctgcgaat cgatctgcgg atgccgcgcc agcgggatca ccggcgcggc
    10801 gcacaggcgc gatgccggga agcgctgcga cacgtgcagt accggcgtca tcgccaccac
    10861 gtcgagcagt gatccggcga agcgcttgtc gcgcaggcgc agggcgtagt cccacaaccg
    10921 cggctgcgcc tgacggaaca gccgggccag gccgatggtg gcgcgcacgt cgctcaaggc
    10981 ctcgtgggcg tcgccgatgc gcaggccgtt gtcctcggcc aggtgctcca gcttgaagct
    11041 ggtgccgccg ccatcctcgc gcggccgcca ggcgatgccg tccgggcgca acgcgtgcat
    11101 cagccgaagc gcgtcgagca ggtcccagcg cgagttgccg ccgcgccatt cgcgttcgta
    11161 gggatcgaag aagttccggt acaggccgtg gcggacgaat tcgtcgtcga agcgcagcga
    11221 gttgtagccc aggctgcagg tttcggggcg cgccatttcc tcgacgatgc gggcgcaggc
    11281 ttcggcttcg ttgacgccat cgcgcaaggc gtcctgcggg gcgatgccgg tgatcatcgt
    11341 cgcgatgggc gagggcagaa ggtcgttcgc cggctgcacg aagaacgaga tcggttcgtc
    11401 aacctggtcg agcgcggcat cggtgcggat cgcggcgaac tgggcgatgc gggtccggcg
    11461 cgggtcggcg ccgaaggtct cgaggtcgta gaacaggaag ctggcggtca tggctggatt
    11521 gtaagagcgg tggctcgcat gcgcggacgc ctaccctcgt gggttcccta cgccgttcga
    11581 aatgcggctg cgcgaatccc tcgatggcct cgtcggcgcg caaccgctca ggcgatcggg
    11641 tccaggcgct cgagcaccgc ggcatccgcc gcgtcccaat cgacctcgtg cagcgacgcc
    11701 aggccacgcg tcgcgtcttc aaggatgacg cgctgcacct cgctgcgatg cagggcgacc
    11761 gaatacggga tgcgcatgaa gctgaccatc gcgtaatgcg gcacgaagcg accgggatgg
    11821 cggtcctgca gcgcgagttc gacctggcgt tgcagcagga acgaggcatc gtccaccttg
    11881 tcgcgcatct cgatgtagtt ctccagcgcc atctgctgga tcgcttcggc atccggcttg
    11941 cgctgcgcct cgaacgcggc gaacgcgtcg gacagcgagg cgttggcgtc gaggtgatcg
    12001 gccagtgcca cgcaatcctc gaacgcgcaa ttcatcccct ggccgtggaa cggcaccatc
    12061 gcgtgcgcgg cgtcgccgat caggaccgcg tggccgtgca ggtgccagtg gtcgaggtaa
    12121 agcgtgccca ggatcccgac cggattcgct tcgtaatcgt ggcgcagttc cgggatcaat
    12181 ggcagcgtat cggggaagtc gcgttcgaag aatgcctgcg cctcgtccgc gtcgcgcacg
    12241 gtctcgaaac tcgggtgcgc gccggcatgc ggcatgaaca gggtgacggt gaaggtgcgc
    12301 tcgtcgttgg gcagggcgat gcacatgtag tggccgcgtg gccagatatg cagggcgttg
    12361 ggctcgatgc ggaagccgcc atccttcgct ggcgggattt cgagctcctt gtagccgtgg
    12421 tccagccatt ccgtgcgctc acccagcggc aagaccttgt tgatttcggc gcgcaggctg
    12481 gagccggcgc cgtccgcacc gaccagggcg cggaaggggc gtcgggtttc atggccgtca
    12541 cccttgaaca ccgcgacctt gtcgacgaaa tccaccgacg ccaggccgtg ctcgaaatgc
    12601 aggcgcgcac catgcgattc ggcgatgtcg agcaggacga tgttgagctc gccgcggttg
    12661 atcgaccaga tcacttcgga atcgtcgcga ccgtagcgtt gcagcccggg ttcgccgtgc
    12721 agcggatgca cgtaacggcc gcgcatcatc accgcctgct tcatcaccgc ggcgtccgcg
    12781 ccagcctggc gcagtgcatg caggccgcgc tcggccaggg caaggttgat cgaacggccc
    12841 ccggcgtagc catgcacgcg cggatcgccg cggcgttcgt acacgtccac cgaccagccg
    12901 cgctgggcca gcaggatggc gagcaacgcg cccgccagcc cgccgccgac gatggtgatc
    12961 tcgcgctggt cagcggtcat cgcgccactc ccgtaccgtg ttggcgaagc gcaggacatc
    13021 cgcatgggtg ttgtacagcg gcactggcga gatccggatc acgtccggtt cgcgccaatc
    13081 gccgagcacg ccgcgcgatg ccaggaactc gaacaagtcg cgtccgcgtt cgcgtccacc
    13141 gatgacgcga atcgacaact ggcaaccgcg tcgcgcagct tcgcgcgggg tgacgacctg
    13201 gagcacgtcg cgcaagccgg catcgatcag ttgttccagg taaccggtca gcgattccga
    13261 tttcgtccgc agcgccggca tcgtggcgcg gtcgtactgg tcgagcgacg cacgcaacgg
    13321 cgccaggccg aggatcggcg ggttcgagag ctgccagccg tccgcaccgg gcgtgggatt
    13381 gaactctggt cccatcctga aacgcacgcc cgggtcgttg ccccaccagc cggcgaagcg
    13441 cgggcggttg gtgcgcgcat ggcgttcatg gacgaagcat ccagcgaccg cgcccgggcc
    13501 ggaattcatg tacttgtaat ggcaccagac cgcaaaatcg gcgccgctgt cgtgcagctg
    13561 cagcggcagg ttgcctactg catgggccag atcaaagccg acgatggcac cggccgcatg
    13621 cccgagccgc gcgatctcct ccaggtcgaa ggcctggccg gtgcggtact gcacgcccgg
    13681 ccagacgatg catgccaggc gcgatccgtg ctcggcgatg gcgcgttcga tcgcggccat
    13741 cgagaaggtg ccatcggcct cgtcgggtac gacctcgacc agcgcttccg ccggatcgaa
    13801 gccgtggaac cgcagttgcg attccagcgc gtaacgatcc gacgggaatg cgccggcttc
    13861 catcagcagt accgggcgct cgcgggtcgg ccggtagaag gtgaccagca tcaggtgcag
    13921 gttggcggtc agcgaattca tcgccaccac ttcccggggc tgcgcgccga ccacgcgcgc
    13981 cagcgggtcg cgcaccagct cgtggtacgg catccactgc gcctggccgg tgaagtggcc
    14041 ttccacggcc tgcgtcgccc atttgtcgag cacttcctcg acatgcgcgc gcgcgccctt
    14101 cggctgcagg cccagcgagt tgccgacgaa gtaggcctgc ggtttgccgt catgcagcgg
    14161 caggtggaat tcgccgcgga agtcgcctag tgggtcgaat gcatcgcgcg atgccgcgta
    14221 tgcgtcggag aaaatctcgg tcatgcgaac ctcgaggccg gcagcccgag ccattccagc
    14281 gcggtgccgt ggtagatgcg ggcgcgttgt gcctcgtcca gggccaggcg ttcgatgcct
    14341 tcgcccggta cctgttcgcc gagggggaac ggataatcag tgcccagcat cacccggtcg
    14401 atcccgcagg cctcgagcag gtagcgcagg gcggcatcgt cggcgaccca ggaatcgaag
    14461 tacaggcgct tcaggtactc gcgcggatta cggaaattgt ctgtcgccac gaggtccggg
    14521 cgcatgttga aaccgtgctc gatgcggccg atggtgtacg ggaagctgcc gccgccatgc
    14581 gccatgcaca cgcgaagtct cggcaggcgc tccagcacgc cgccgaacac caggcagcag
    14641 gccgcgcgcg actgctccgc cggcatcccg accagccacg gcagccagta tttcggcatc
    14701 gtgtcggtgc ccatcatgtc ccatgggtgc accaggatcg ccgcacccag gtcgctggcg
    14761 gcctggaaga agtcgaacaa ctccggcgcg tcgaggttcc agtgcgcgtc gcccgggccg
    14821 ttgaggtgcg aaccgatctg cacgccctgc aggccgagtt cgtccatgca gcgttccagt
    14881 tcgcgaatgg cgagctgcgg gctctgcagc ggcaccgtgc cgatcccggc gtagtgacgc
    14941 ggatagtcgc gacaggtccg tgccatgtgc tcgttgagcg acttgtgcag ttccagtgcc
    15001 tggtgcggct tggcccattc gctgaacatc accggcaccg tgctcagcac ctgcacctgc
    15061 acgccgaaac cggcgtaatc gtcgatgcgg atctgcggat cccaggtctt cggccagatc
    15121 tcgcggaaga acttgccgtc cttgtagatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnng tgatgtccgc ctgcgactgc gcatccatct cgtagcgcgt gggtcgcgga
    15301 ttgagcgtgc cgcatgcctt gcaggtgcgc agcgcgtcgt cgcgatagaa ccgctcgaac
    15361 acccggaaga aatcctgctc gatgtcgacc aggtggaaga actcctcgta cagcgtgtgg
    15421 ttgcaggcga tgcagaacca catcagcgca tcgtcctcgt gcgccagccg cttgcgctcg
    15481 atcacgaggc cgatgctgcc cggcatgcgc tgcggcgaat gcgggacatg cggcggcagg
    15541 tagaacatct cgccggcacg gatcgggatg tcgcgggcgc ttccatcttc ctggatgcgc
    15601 aggaccatct cgccctcgag ttggtagaac cactcgggac cgtcctcgta gtgatagtcg
    15661 gtgcgcgcat tcggcccgcc gacgatcatc acgatgtagt cggcgtcgac gatgcacttg
    15721 ttgccgaccg gaggtttcag caggtggcgg tgttcgtcga tccagccttg caggttgatc
    15781 gggttcggca gcatgtcagt ccttcagcac cgcgatgcat ttgagttcga tcgcgatcgg
    15841 cgtgggcagt gcggtgatgc ccagcgtggt gcggcagggc gccgtcgcgg catcggggaa
    15901 gtgttccgcc cagagtgcgt tgtaggtctt gaagtcgcgg gccatgtcgg tgaggtagac
    15961 ggtgacgtcg accaggtcgc cccatgtcgc gccactggcg tccagcacgg cgcgcacgtt
    16021 ggcgaacacg gcgcgtgcct gtgcctcgat gtcgtatttg cgcaggcggc cgtcggcgaa
    16081 gtagtcgttg ccgggaatcg tattgtcgac cgggttgcgc gggccgatcc cggacaggta
    16141 cagcgtgttg ccgacccgtc gcgcatgcgg atagcttcca acggctttcg gcgcggcatc
    16201 ggcgtggatc gaacgatgct ggtccgggcc cgacatcagc ggtcttcctt gtccacgctg
    16261 atcgcggcga gcgcagcggc gtattgcggc tccaactggt cggacgcggc cacgttgaga
    16321 ccaaggtcgt gcacgcggcc atcgcgcagc gtgtacaccc aaccatgcac cgacagcgtt
    16381 tgtccccgca cccaggcatc gcggacgatg gtggtctggc agacgttggc aacctgttcc
    16441 agcgcattca attcgcacaa gcggtcgtgc tgcaggggtt cgtcgccggc atccagcagc
    16501 aactgcgcat gcttggtgga gacatcgccg acgtggcgga tccagttgtc ggccaagccc
    16561 acccgcgcat tggtcaaggc cgcatgcacg ccgccgcagc cgtagtggcc aaccaccagg
    16621 atgtgctcga ccttgagcac gtccaccgcg aactggatca ccgacaggca gttgaggtcg
    16681 gtgtgcacga cgacgttggc gatgttgcga tggacgaaca cttcgccggg cgcgaggtcg
    16741 accacctggt tcgccggcac ccgcgaatcc gagcagccga tccacaggta tttcggtgcc
    16801 tgctgcttcg acaggcgttc gaagaagccg ggatcctcgc gcgagacacg ctcggcccag
    16861 gcttggttgt tgcgcaggag atcgtcgagt cggttcatgg ggtctttgcg aaaggtaggt
    16921 tggcgtcggg ggagggaact tgcagggggc gatcgagcag cgtgccgcca tccgcgccag
    16981 ccaggcgcag gcggtacttt ccgggggcga gatagagcgg ggtggaagcc tctgaagtgg
    17041 cgagcatcag gaactggtcg gccgggatgg cctcgtcgcc ttcatcggca tggcgcgcct
    17101 ccgcaaggca gggcaggtgt ccggcacacg gcgtcaggtc gatggcgacc accccgcgcc
    17161 tgccaccgag cgacagccaa tcggggcgtt gtgcctgccc cgcgggggcg gccggcaaca
    17221 gcacggaaac atcattgaga ccggggcggt agctccaggg gccagcgccg tcgcgcgaca
    17281 gcagcacgct cggcgcgctc aacgcgaact gcctggccaa gcgctggccg accgtgtcga
    17341 tgtccgcagt ttcgtaggag cgcgtcgagg tctggtcgat ggtcaacact ggcagcccgg
    17401 tcagccgtgc cagttccatc gccatcggcc tcgcgtcatc gggaaggcct tgttgcgcct
    17461 cgccgatgtg cgcatagccg gcatgcacca gcaccttcgc gctgggttcc ctcgccaaca
    17521 agtcggcgat tgcctgggcc tggccggtct cgcggtcctg ctgtcgttcg cctggcttgt
    17581 tgggtgtctc gtagggaacc agccgatagc cgaggcggag cgcctcgcgc agcagctcgg
    17641 cgaacaccgg gtcgcgggta tagatgccgg tcttgcgcac cggatagccg ctcggcattg
    17701 gatcggtacc gtcgttgacc agtgcttcga cggcgaggtg ggtgtaaccc tgttcgcgca
    17761 ggggttgcag gagggccagt gtcagtacgc gcgtgtgcgg cgcatggtgc gcttcgttga
    17821 gcatgaccac gcggtagtcg ccggcctgtg cagagatcca ctcgctggcc ggcagggccc
    17881 tccagtcgga tggcaggggc aggtcgcttt ccggcgcgct gttgccgcgg taggggaagg
    17941 cacgcagggc atccatcggc cggccgagga agctcaggtg ggtcgccagc atctgcgatg
    18001 ccatgttgcg tgtctgcgcg tcctgctgga cggcagggct ggttaggaac cgatattcgg
    18061 cgaaggtatc gggttgtgcg ttgaaggcct tgaagaaatc gtcgaccgga ttcttcgttc
    18121 catccccgcc ggccgatact gcgttggcag ccagtgcgag ggataggagg gcgatggaaa
    18181 ccgcgcgctt gttcatggcg gcagcgcgat gccgacgttg cgtggttcgg tgaagaagcg
    18241 catcgcctcg agcccgcctt cgcggcccat gcctgatgcg ccgctgccgc cgaacggcgt
    18301 gcgcaggtcg cgcatcagcc aggtgttgat ccagaccatg cccacgcgca attgcgcggc
    18361 catgcgatgg gcgcgcgaga ggtcgttggt gaatacggat gccgacagcc cgtagtcgct
    18421 ggcattggcc agcgcgaggg c
//
LOCUS       DAOOCX010000043        17938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2782_length_17938_cov_7.863166, whole genome shotgun sequence.
ACCESSION   DAOOCX010000043 DAOOCX010000000
VERSION     DAOOCX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 17938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17938
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2782_length_17938_cov_7.863166"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="PK399_05880"
     CDS             <1..178
                     /locus_tag="PK399_05880"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302579.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPW12680.1"
                     /translation="DQPHRLNTSSRRTVYGLSPFEGTTFHSRIAATWVNGVLGWDGER
                     LVGTPQGQRLQFDR"
     gene            262..1041
                     /locus_tag="PK399_05885"
     CDS             262..1041
                     /locus_tag="PK399_05885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW12681.1"
                     /translation="MIGGDGAIPMRATRLPATASQGAMVIGNTHPAATVDYAGRTLRV
                     TPYGTFVFGIGRDATGEVVLRIKQPATAWIEHRVAITPRDWPVENINGVPPATVDPPP
                     AIAERIEREQARVVAARIRDDARTGFAQAFAWPVEGRISGRFGNQRVYNGTPKSPHSG
                     MDIAAPTGTPVKAPADGVVTFADPGLYLTGGTLVLDHGHGISSNFLHLSRIDAKVGDV
                     VKQGDVIAAVGATGRATGPHLHWGMNWFDVRIDPLLVLERK"
     gene            complement(1172..2095)
                     /gene="folE2"
                     /locus_tag="PK399_05890"
     CDS             complement(1172..2095)
                     /gene="folE2"
                     /locus_tag="PK399_05890"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPW12682.1"
                     /translation="MHNTTRHASLPDVAFDTAALARPLDWVGMDAMALPIRLPGEGSQ
                     ALHVPASVDVAVDLRDGAARGIHMSRLYLQLQQSLANEALTAPALRKLLETCIATQQG
                     LSTRARLRLRYDHLLLRPALASANAGWKNYPVTLEAELRDGHLQLSLAFSVDYSSTCP
                     ASAALSRQVNAARFEADFTGVRALSGETVRDWLASERGLAATPHAQRSRAEIRVELRP
                     AFDEAPLRALVDVVEAALGTPVQTAVKREDEQAFAELNAANLMFCEDAARRVAAALAD
                     DARVAAWQVRVAHFESLHPHDAVASVSGRNA"
     gene            complement(2157..2843)
                     /locus_tag="PK399_05895"
     CDS             complement(2157..2843)
                     /locus_tag="PK399_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HPW12683.1"
                     /translation="MSETDALRGFLGKWEAQWPEWKVAEAFVPVSQRERAQAWFALRE
                     ELAEAAWGEGDARPGDAKLGWWAEELDGWSRGLRRHPLGLTLQKLPAPWANLAACLPA
                     LRASRERPQGLEPAMFALEPYAEALAGVAQHLFDSATPAPARNVVVSLLAERVLRHPG
                     AATPLGDLDVRGWARGLLAQWPAPAQGSRPGRIHAAIVRGRLQRFAGGKPVPMSRPGV
                     LFTAWRAARG"
     gene            complement(2840..3517)
                     /gene="gph"
                     /locus_tag="PK399_05900"
     CDS             complement(2840..3517)
                     /gene="gph"
                     /locus_tag="PK399_05900"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534888.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HPW12684.1"
                     /translation="MRAGMFPRLVLFDLDGTLLDSAPDMVATVNRMRAARGQGPMPLA
                     ELRPHVSKGSRAMSAAAFPELGGEVSGEMIREFLDTYEQELGRSSVLFDGVAELLDAI
                     EADGARWGIITNKPEYLARQVMPQLGWGTRSAILLGGDTLAERKPHPLPLLHAAGQLD
                     AAIDDCVYIGDDQRDIEAARAAGMRSVVALWGYRDEGERPDGWGADALADTPVDLLAP
                     TFWARWR"
     gene            complement(3514..4239)
                     /gene="ubiG"
                     /locus_tag="PK399_05905"
     CDS             complement(3514..4239)
                     /gene="ubiG"
                     /locus_tag="PK399_05905"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HPW12685.1"
                     /translation="MTASARNFSQAELDKFDELAQRWWDPDGPQKALHALNPARLGYV
                     ADRTPLRDAAVLDVGCGGGLLSEALARAGARVTAIDLAPNLLKVARLHGLESGIKVDY
                     RELPVEALAEEMLAEGRPASFDAITCMEMLEHVPEPGSVIRACATLLKPGGRLFLSTL
                     NRTPAAFALAIVGAEYVARLLPKGTHQYRDFIKPSELAKWLREAGLELEDVSGLMYEP
                     WRNSARVIARTDINYLAYARKPG"
     gene            complement(4236..5576)
                     /locus_tag="PK399_05910"
     CDS             complement(4236..5576)
                     /locus_tag="PK399_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HPW12686.1"
                     /translation="MTDPVPQACDLIIEAGWVVPVVPHGVVLTDHAVVVSGGAIVAVL
                     PIAEARTRFEAKESVSRPDAALIPGLVNAHTHNPMTLLRGVADDLPLKVWLQEHIWPI
                     EGAVIGPGFVADGVTLAIAEMLRGGTTCCNENYFFPDVQAATYKRHGFRARVGLPVID
                     FPTAWASSDDEYFDRAGEVHDQWRDDALIRTAFAPHAPYTVNDANFERVRMLADQLDV
                     PVHLHLHETAQEVQQSIEQHGQRPIARLDRLGLVNDRLIAVHMTQLTEAEIHLCAERG
                     VSVVHCPESNLKLASGFCPACALERAGVTLAIGTDGCASNNDLDMVGETRTAALLAKA
                     VASDAAGFDAHAALRAATLGGAKAIGFDHLVGSLEPGKRADIACVDFSALETQPLHNI
                     VSQLIYATGRHQVSDVWIEGDQKLRRRVLVDMDLDGIIANARQWRDRIATVRPA"
     gene            complement(5581..6147)
                     /gene="efp"
                     /locus_tag="PK399_05915"
     CDS             complement(5581..6147)
                     /gene="efp"
                     /locus_tag="PK399_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPW12687.1"
                     /translation="MASLGMNDVKTGQKILINNDPCVITETEYVKPGKGQAFTRIKYR
                     SIKSGRVVELTMKATDNVEQADVVDTDMQFLYSDGEYWHFMNQESFEQVQSDKAGMGG
                     AEKWLKGEEECVVTLWNGTPIAVQPPNFVELKITETDPGVKGDTAGTGGKPATLETGA
                     VVRVPLFVGQDEKIKVNTRTGEYESRVK"
     gene            6240..7301
                     /gene="epmB"
                     /locus_tag="PK399_05920"
     CDS             6240..7301
                     /gene="epmB"
                     /locus_tag="PK399_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HPW12688.1"
                     /translation="MITAAPVALQPTATSPPPALRWQQAWRDAVRDPRELLAMLGLGH
                     LAAQLSDDAARQFPLRVPRGFVARMRHGDPGDPLLRQVLPVLEEDRIVPGFGLDAVGD
                     GAARRGGGVIHKYNGRALLVATGSCAVHCRYCFRRHYPYAEDTAAAAGWREAVETIRA
                     DDSLHEVILSGGDPLSLADHKLAELTDALRGIPHIRRLRLHTRLPIVLPERVDADLLA
                     WLRGLPWPVAVVVHANHAHEFDGAVDKAMAALRDAGATLLNQAVLLRGVNDAVDTLAA
                     LCERGHAAGVLPYYLHQLDRVEGAAHFEVDDAQALALHAALAARLPGYLVPKLVREVA
                     GAPGKTPLQAVDAGLPVVG"
     gene            7341..9422
                     /locus_tag="PK399_05925"
     CDS             7341..9422
                     /locus_tag="PK399_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW12689.1"
                     /translation="MAIGKDTALRLMLVDDQVNEAEAIVSSLRNAGIAVRPLRAESLD
                     ELAQILSQQPVDMVLAEYASTQMPFAEVAAVVMGCGRDVPLLAVLDGITDEILDSVQA
                     QGAQTVALRGRPQQFLKNVRTEWHDLDARRSQRHLEAQMRETERRCDMLIDSSREPIA
                     YIHEGMHIRANQAYLEMFGYETFEDIEGMSLLDFIAPKDVEEFKALLKRLSKGEPPPP
                     RHELTARDIDGTDFPAIMEFTAAQYEGEACQQVIFRRQATEQDPELARQVEELKQRDP
                     ATGLLNRPTFLGLLDGAVAEAAGQGAAHGLLLIEPDHYAQLLDVIGLDAADDLMAAMG
                     QRLDSVANDAITARFGEHQLALLVRGSDYRATSDLAERVREAFAGHVLEVGDNTLNAT
                     VSIGGVQIGEKIASTSQVLAKATQGVVSAVGVGGNRVQLFDPGAVDRAEQERIAAWVA
                     RIQGALDSEGFRLDFQPVIALQGDGSETYEMFLRLHGSDGVPVEASTYLPIAEEHDLL
                     RPIDRWAVSRAVALVGERLRAGKRTSLLLSVSQEALLDSSMPAFIADQLAAAGVPGEQ
                     LILAVPEAKVFTNLHAAQEFAGALAGFGSRMALEHFGAGLNSLQTLQHFEPALLKIDP
                     QFMDDLSKSADSQARVREIAQQAGQRGIRTVAERVQDASSMTLLFSAGVDYVQGDFLA
                     PAGPDMTYDFS"
     gene            complement(9503..10399)
                     /gene="htpX"
                     /locus_tag="PK399_05930"
     CDS             complement(9503..10399)
                     /gene="htpX"
                     /locus_tag="PK399_05930"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HPW12690.1"
                     /translation="MFKRGFLFLATNLAVLALVSIVMSILGVNPRQFGGLLIMAALFG
                     FGGSIISLLVSKWMAKRATGAHVIETPRNEAEQWLLGTVRRQAQAAGIGMPEVAIYDA
                     PEINAFATGANRNNALVAVSTGLLRAMSRDEAEAVLAHEVSHVANGDMVTMALLQGVL
                     NTFVIVLSRVIGRAIDGYLGNRDGYGIGYYLGVFVLDMVFGLFASMIAMWFSRHREFR
                     ADAGGAALAGKQKMIAALERLSQTYGHSSLPKQVAAFGISGVVGNGLKRLLMSHPPLE
                     ERISALRNAQPDAGAVRQGVVA"
     gene            10491..11396
                     /gene="gluQRS"
                     /locus_tag="PK399_05935"
     CDS             10491..11396
                     /gene="gluQRS"
                     /locus_tag="PK399_05935"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HPW12691.1"
                     /translation="MPLPAYRGRFAPSPTGPLHAGSLLAALGSWLLARQAGGEWLLRI
                     EDVDPPREIAGAADGQRAALATFGLHHDGPVIHQSKRGGIYQEALDRLLAKGLAFECH
                     CSRSDLVASGGIHRHCVAAARRPDPAIRLRVPDECEIGFDDLVHGRVVQRVDVDAGDF
                     VLRRADGPWAYQLAVVVDDAAQGITEVVRGADLLDSTPRQILLQRALDLPTPGYAHLP
                     LLLDTDGRKLSKSCAAMPVDPDDPIPALHACWLALGQGALPATIDSTERFLAAAIEAF
                     RIDRVPRDALPASAAAHNTDATSRR"
     gene            11436..12176
                     /gene="phbB"
                     /locus_tag="PK399_05940"
     CDS             11436..12176
                     /gene="phbB"
                     /locus_tag="PK399_05940"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HPW12692.1"
                     /translation="MTSRVALVTGGTGGIGTAIVKRLADMGHKVATNYRNEEKARAWQ
                     EQMKAAGYDIAIVKGDVTSPQEAEAMVREVEATLGPVDILVNNAGITRDGTFHKMKAD
                     QWGDVINTNLNSVFNVTRPVIEGMRERKWGRVIQISSINGLKGQYGQANYAAAKAGMH
                     GFTISLARENARNGVTVNTISPGYIATDMVMAVPEEVRAKIVADIPTGRLGTPEEIAY
                     GVGFLADEQAGWITGSNLDINGGHHMGW"
     gene            12382..12822
                     /locus_tag="PK399_05945"
     CDS             12382..12822
                     /locus_tag="PK399_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPW12693.1"
                     /translation="MSNVDPNRRPSNAGRYAFLLILGLVLGVIATVMTMRALNERKDH
                     FPDALMHVQQWHMGQLKADMDANRCNATDILPHLQALRVTANDIDAAFPDLRDDTRFT
                     AASSAMRAATDKAIANPPLTCDGLGATMKSIGETCKACHQDFRG"
     gene            13055..13528
                     /locus_tag="PK399_05950"
     CDS             13055..13528
                     /locus_tag="PK399_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HPW12694.1"
                     /translation="MTIRTPHIRTLGAAILGLALAAGTGCASTGSSGYSGGPSYGGNY
                     GNTCQNCGTVTRITVGGESGKAGAVIGGIIGAVAGHEISAQTGGSKGNQNISAVAGAA
                     AGAAAGNAIQKNRSDGYTVHVRMDDGRTTAVTQDNVSGFREGSRVRISDGRAYLQ"
     gene            13639..14139
                     /locus_tag="PK399_05955"
     CDS             13639..14139
                     /locus_tag="PK399_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HPW12695.1"
                     /translation="MEPTTLYYVLAALLVVVGIAGTILPALPGLPLVFAGMLLAAWAG
                     NFQQVGVPMLVVLGLLTVFSLAVDFWATALGAKRVGASRLAIIGAMVGTFGGLFLGPL
                     GLLMGPFAGAIGGELIHRRSLLKQDLGHATRIGVGTWFGILFGTVLKLALAFTMLGLF
                     ALAWWL"
     gene            14244..15509
                     /locus_tag="PK399_05960"
     CDS             14244..15509
                     /locus_tag="PK399_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPW12696.1"
                     /translation="MAWTIAITALVTLLLVTLAMNFKTSEKTLERKIEHRYAVSDPQF
                     RREMGVLLGPGIIQGNRVTDLENGDEIFPAMLEAIRGATTTITFETYIYWSGDIGAKF
                     ADALAERARAGVKVKVMVDWVGSIKMEDDLLQRMQDAGVEIHKYRPLKWYNLGRLNNR
                     THRKLLVIDGRIGFTGGVGIADQWEGHAQDTDHWRDLHFRIEGPVVAQVQSAFNDNWI
                     KTTGVVLNGADYFPPLATVGEMDAHMFVASPAGGSESMHLMYLMAIAAAERSIDLEAA
                     YFVPDPLMIQALQAARHRGVRVRVIVPGKHIDSDTVRLASKAHWGELLLAGVEIHEYQ
                     PTMMHNKLLIVDGMMTSVGSTNFDIRSFRLNDEASLNVYDAAFAQRMTEVFEGDLKPT
                     VRYDYRMWKNRSLKEKLFEKFILPFKSQL"
     gene            15519..16547
                     /locus_tag="PK399_05965"
     CDS             15519..16547
                     /locus_tag="PK399_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPW12697.1"
                     /translation="MPILDPDKLPTASSLGERFMQALEQVQDKLLHLVASAPLLLVAL
                     LIVLFASWLGGFASRHLRVVKRFSSSNPYMEGLLRSTVRIVITLAGVVVALDLLNATS
                     LVGAVLGSAGVIGLVLGFGFKDIAENYIAGVLLSLRKPFSPGDTVRIDSFEGKVVALT
                     ARATILMTVDGNHLQLPNSVVFKSVLLNYSRNPKRRFDFETVVDNRASWHAAMDAGLA
                     AIGSVEGVLAEPPPSTLIRTLSNDGATLQFAGWIDQRHNDLAKTRSEAMRRVRRELRE
                     AGFVPPEHVQKVILQRGEADFPQAQENGHARDTSVDRALDAQVGNARRLEDGGDLLRP
                     PPPGSPPT"
     gene            complement(16549..16944)
                     /locus_tag="PK399_05970"
     CDS             complement(16549..16944)
                     /locus_tag="PK399_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12698.1"
                     /translation="MSNTRVVNGPGFKVCYEDEPGYLRAYVFDGTDSVDISIAMWRML
                     AAEGGAVGATRMLILEDLVSTVGLDDLAKVIDAIEAAGMGRFRSAFVELRDDIQGSEL
                     GEIMLQERGITIRIFANENEARHWLLYGG"
     gene            complement(16941..>17938)
                     /locus_tag="PK399_05975"
     CDS             complement(16941..>17938)
                     /locus_tag="PK399_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HPW12699.1"
                     /translation="MGAGWCPPGMLGIGIGGTAEKAMLLAKESLMEHIDIQDLIERGP
                     SNRVEELRIELYEKVNALGIGAQGLGGLTTVLDVKIKDYPTHAANLPVAMIPNCAATR
                     HAHFTLDGTGPVALEPPSLEDWPTLTYNPQNARRVDLDRITREDVLTFKPGEVLLLNG
                     KMLTGRDAAHKRIVEMLDKGEPLPVDLAGRFIYYVGPVDPVRDEVVGPAGPTTATRMD
                     KFTEQVLAQTGLLGMIGKAERGPVALEAIRKHQSVYLMAVGGSAYLVSKAIKAARVVG
                     FADLGMEAIYEFEVKDMPVTVAVDSQGTSVHQTGPKEWQARIAKGMIGGIPVVVA"
BASE COUNT         2886 a         6288 c         5926 g         2838 t
ORIGIN      
        1 cgaccagccg caccggctca acacgtcctc gcgtcgcacg gtgtacggct tgtcgccttt
       61 cgaaggcacc acgttccact cgcggatcgc cgccacctgg gtcaatggcg tgctgggctg
      121 ggacggcgag cgcctggtcg ggacgccgca gggccagcgt ttgcagttcg accgttgatg
      181 cgccttcccc tggcgttcct gcttgccctg gcgctgaccg gcatcgccag cgcgcaatcg
      241 gtgccgacgg aagccgggcc gatgatcggc ggcgacggcg ccattccgat gcgcgccacg
      301 cgcttgccgg caaccgcatc gcaaggcgcg atggtcatcg gcaacacgca tcccgccgcg
      361 accgtcgact acgcgggacg cacgctgcgg gtcacgccct acggcacctt cgtgttcggc
      421 atcggccgcg acgccaccgg cgaggtcgtg ctgcggatca agcagccggc gaccgcctgg
      481 atcgagcatc gcgtcgccat caccccacgc gactggccgg tcgagaacat caacggcgtg
      541 ccgcccgcca cggtggatcc gccacccgcg atcgccgagc gcatcgaacg cgagcaggcg
      601 cgcgtggtcg cagcgcgtat ccgcgacgat gcccgcaccg ggttcgcgca agcgttcgcc
      661 tggcccgtgg agggtcggat cagcggccgc ttcggcaacc agcgcgtgta caacggcacg
      721 ccgaaatcgc cgcattcggg catggacatc gccgcgccga ccggcacccc ggtgaaggca
      781 ccggccgatg gcgtggtcac cttcgcggat ccgggcctgt acctcaccgg cggcaccctg
      841 gtgctcgacc acggccacgg catcagcagc aatttcctgc acctgtcgcg gatcgacgca
      901 aaggtcggcg acgtggtgaa gcagggcgac gtcatcgccg cggtaggcgc caccggacgc
      961 gcgaccggcc cgcacctgca ctggggcatg aactggttcg atgtgcgcat cgacccattg
     1021 ctggtgcttg aaagaaagta gcgttcatct cctccccttg cgaagcagga ggagattggg
     1081 aaagggtgcc ttcatcgcac atcccgacca gcaacacccc accccagctc tcccctgctt
     1141 cgcacgggag ggagcaaaag ccgtcagtcc gtcaggcgtt ccgcccgctc acgctggcca
     1201 ctgcatcatg cggatgcagg ctctcgaagt gcgcgacgcg cacctgccag gcggccacgc
     1261 gcgcatcatc cgccagcgcg gcagccacgc gacgcgcggc atcctcgcag aacatcaggt
     1321 tggcggcatt gagttcggcg aaggcctgtt cgtcctcgcg cttgaccgcg gtctgcaccg
     1381 gggtgccgag ggcggcttcc accacatcca cgagcgctcg caaaggcgct tcgtcgaagg
     1441 caggccgcaa ctcgacgcgg atctcggcgc ggctgcgctg cgcatggggg gtggcggcca
     1501 ggccacgttc cgaggcgagc cagtcgcgca ccgtttcgcc cgaaagtgca cgaacgccgg
     1561 tgaaatccgc ctcgaaacgt gccgcgttga cctgccggga cagcgccgcg gacgccgggc
     1621 aggtacttga atagtcgacc gagaaggcca gcgacagctg caggtgcccg tcacgcaatt
     1681 cggcctccag cgtcaccggg tagttcttcc agccggcgtt ggcgctagcc agcgccgggc
     1741 gcaacagcag gtggtcgtag cgcaggcgga ggcgcgcacg ggtcgacagt ccctgctggg
     1801 tggcgataca ggtttccagc agcttgcgca gcgcgggcgc agtcagcgct tcgttggcca
     1861 gcgactgctg gagctggagg tacagccgcg acatgtggat gccgcgcgcg gcgccgtcgc
     1921 gcaggtcgac cgcgacgtcc acgcttgccg gcacatgcag ggcctggctg ccctcgcccg
     1981 gcaggcggat cggcagcgcc atcgcgtcca tgccgaccca gtccagcggg cgcgcgagcg
     2041 cggcggtgtc gaaggcgacg tcgggcagcg aggcatggcg ggtcgtgttg tgcatcgccg
     2101 cattgtaccg gcgcggcggt gagcgcctgc gcaacgctgc gtgccgcggg gcaggctcag
     2161 ccgcgggcgg cgcgccaggc ggtgaacagc acgccgggcc ggctcatcgg taccggtttg
     2221 ccgccggcga atcgctgcag gcggccgcgc acgatggcgg cgtggatacg gcccgggcgg
     2281 ctgccctgcg ccggcgcggg ccactgcgcc agcaggccgc gggcccagcc gcgcacgtcg
     2341 aggtcgccaa gtggcgtcgc cgcgccgggg tgacgcaaca cgcgttcggc caggagcgag
     2401 accaccacgt tgcgcgccgg tgccggcgtg gcgctgtcga acaggtgctg ggccacgccg
     2461 gccagcgcct ccgcgtacgg ttccagcgcg aacatcgcgg gttccaggcc ctggggacgc
     2521 tcgcggctgg cgcgcagggc cggcaggcag gccgccaggt tggcccacgg cgcgggcagt
     2581 ttctgcaagg tcaggcccag cgggtgtcgg cgtagcccgc gtgaccagcc atcgagctct
     2641 tctgcccacc agcccagctt ggcatcgccg ggacgcgcgt cgccctcgcc ccaggcggct
     2701 tccgcaagtt cctcgcgcag ggcgaaccag gcctgcgccc gctcgcgctg cgacacgggc
     2761 acgaacgcct cagccacctt ccattcgggc cactgcgctt cccatttgcc caggaagccc
     2821 cggagtgcgt cggtttcgct catcgccatc gcgcccagaa cgtgggggcc agcaagtcga
     2881 ccggcgtatc cgccagcgcg tcggcgcccc agccgtccgg gcgttccccc tcgtcccgat
     2941 acccccacaa cgctaccacc gaacgcatgc cggccgcccg cgcggcctcg atgtcgcgct
     3001 ggtcgtcgcc gatgtagacg cagtcgtcga tggccgcgtc cagctgtccc gccgcgtgca
     3061 gcaggggcaa tggatgcggc ttgcgctccg ccagggtgtc gccaccgagc aggatcgcgc
     3121 tgcgcgtccc ccaccccagt tgcggcatca cctgccgggc caggtattcg ggcttgttgg
     3181 tgatgatgcc ccagcgcgcg ccatcggctt cgatggcatc cagcagttcg gccacgccat
     3241 cgaacagcac gctgctgcgg ccaagctcct gctcataggt gtccaggaat tcgcggatca
     3301 tctcgccgga cacctcgcca cccagctccg ggaaggccgc ggcgctcatc gcccgcgagc
     3361 ccttcgatac atgcgggcgc agttcggcca gcggcatcgg tccctgcccg cgcgccgcgc
     3421 gcatgcgatt gacggtggcg accatgtcgg gcgcgctgtc gagcagggtg ccgtcgaggt
     3481 cgaacagcac caggcgcggg aacatgcccg ccctcatccg ggcttgcgcg cgtaggcgag
     3541 gtaattgatg tcggtgcgtg cgatgacgcg agcactgttg cgccatggct cgtacatcag
     3601 gccgctcacg tcctccagct cgaggcctgc ctcgcgcagc cacttcgcga gttccgacgg
     3661 cttgatgaaa tcgcggtact ggtgtgtgcc cttgggcagc agccgcgcca cgtactcggc
     3721 cccgacgatg gccaacgcga acgccgccgg cgtgcgattg agggtggaga ggaacaggcg
     3781 gccgcccggc ttcaacagcg tggcgcaggc ccggatcacc gaccccggct ccggtacgtg
     3841 ttccagcatt tccatgcagg tgatcgcgtc gaacgatgcc ggcctgccct cggcaagcat
     3901 ctcctcggcc agggcttcga ccggcagctc gcggtagtcg accttgatgc cggactcgag
     3961 gccgtgcagg cgcgcgacct tgagcaggtt cggcgcaagg tcgatggcgg tgaccctcgc
     4021 gccggcccgt gccagcgcct cgctgagcaa gccgccgccg caaccgacat cgagtaccgc
     4081 tgcatcgcgc aacggcgtgc ggtcggccac gtaacccagg cgcgccggat tcagcgcatg
     4141 caaggccttc tgcgggccgt ccggatccca ccagcgttgc gccagttcgt cgaacttgtc
     4201 gagttcggcc tgcgagaaat tccgggcgga tgcggtcatg ccggcctcac tgtggcgatg
     4261 cggtcgcgcc attggcgcgc attggcgatg atcccgtcca ggtccatgtc gaccagcacg
     4321 cgccggcgca gtttctggtc gccctcgatc cagacatcgc tgacctggtg gcggccggtg
     4381 gcgtagatga gctgcgagac gatgttgtgc agcggctggg tttccagcgc cgagaaatcc
     4441 acgcaggcga tgtccgcacg cttgccgggc tccaggctgc cgaccagatg gtcgaaaccg
     4501 atcgccttcg caccgcccag cgtggccgcg cgcagcgcgg catgtgcgtc gaagcccgct
     4561 gcatcgctgg ctaccgcctt cgccagcagc gcggcggtgc gggtctcgcc caccatgtcg
     4621 aggtcattgt tggaagcgca accatcggtg ccgatcgcca gggtcacgcc cgcacgttcc
     4681 agggcgcagg cggggcagaa accggaggcc agcttgaggt tggactccgg gcaatgcacc
     4741 acgctgacgc cgcgctcggc gcagaggtgg atctcggcct cggtgagctg ggtcatgtgc
     4801 acggcaatca ggcggtcgtt gaccaggccc agccggtcca gccgcgcgat cggccgctgg
     4861 ccgtgctgtt cgatggactg ctgcacttcc tgcgcggttt cgtgcaggtg caggtgcacc
     4921 ggcacatcca actggtcggc gagcatccgc acgcgctcga agttggcgtc gttcacggtg
     4981 tacggcgcat gcggggcgaa ggcggtgcgg atcagcgcgt cgtcgcgcca ctggtcgtgc
     5041 acttcgccgg cacggtcgaa gtattcgtcg tcgctgctcg cccaggcggt cgggaaatcg
     5101 atcaccggca ggcccacccg cgcacggaag ccgtggcgct tgtaggtcgc ggcctgcacg
     5161 tcggggaaga agtagttctc gttgcagcag gtggtgccgc cgcggagcat ctcggcgatc
     5221 gccagggtga cgccatcggc gacgaagccg ggaccgatca ccgcgccctc gatcggccag
     5281 atgtgttcct gcagccagac cttcagcggg aggtcgtcgg cgacgccgcg cagcagggtc
     5341 atcgggttgt gggtgtgcgc gttgaccagg ccggggatca atgcggcgtc cgggcgcgac
     5401 acgctctctt tcgcctcgaa gcgcgtgcgc gcctcggcga tcggcagcac cgcgacgatg
     5461 gcgccgccag acacgacgac ggcgtgatcc gtcaggacca cgccgtgcgg caccaccggc
     5521 accacccacc cggcttcgat gatgagatcg caggcctgcg ggacgggatc ggtcatcggc
     5581 ttacttgacc cgcgactcgt actcgccggt cctggtgttg accttgatct tctcgtcctg
     5641 gccgacgaac aacggcacgc gcaccaccgc gccggtttcc agggtggccg gcttgccgcc
     5701 ggtgccggcg gtgtcgccct tcacgccggg atcggtctcg gtgatcttga gttcgacgaa
     5761 gttcggcggc tgcaccgcga tcggggtgcc gttccacagc gtcaccacgc actcttcctc
     5821 gcccttcagc cacttctccg cgccgcccat gccggccttg tcggactgca cctgctcgaa
     5881 cgattcctgg ttcatgaagt gccagtactc gccatcggaa tacaggaact gcatgtcggt
     5941 atcgaccacg tccgcctgct cgacgttgtc ggtcgccttc atggtcagct cgaccacgcg
     6001 gccggacttg atgctgcggt acttgatgcg ggtgaaggcc tggcccttgc ccggcttgac
     6061 gtattcggtc tcggtgatga cgcagggatc gttgttgatc aggatcttct gaccggtctt
     6121 gacgtcgttc atgcccaggg aggccatggg tgctccttcg cgccacccag ccgggcagct
     6181 aaaatgtggg atcagggatt ccggcgtggc cggaacgtgt cgttggatcg cagacccaca
     6241 tgataaccgc tgcccccgtg gccttgcagc ccaccgccac atcgccgccc cccgccctgc
     6301 gctggcagca ggcgtggcgc gatgccgtgc gtgacccccg cgagttgctg gcgatgctcg
     6361 ggcttggcca cctcgccgcg cagctctccg acgacgcagc ccgccagttc ccgctgcgcg
     6421 tgccgcgcgg cttcgttgcc cgcatgcgcc acggcgaccc gggcgatccg ttgctgcgac
     6481 aggtgctgcc ggtgctggag gaagaccgca tcgtccccgg ctttggcctg gacgcggtcg
     6541 gcgatggcgc ggcccgacgc ggtggcgggg tgatccacaa gtacaacggg cgggcgctgc
     6601 tggtcgccac cggcagctgc gccgtgcatt gccggtactg cttccgccgc cattacccgt
     6661 atgcagagga caccgccgcg gcagccggct ggcgcgaggc cgtggagacg atccgcgcgg
     6721 acgattcgct gcatgaggtc atcctgtccg gcggcgatcc gttgtcgttg gccgaccaca
     6781 agctggcgga actgaccgac gccctgcgcg gcatccccca catccgccgc ctgcgcctgc
     6841 atacccgcct gccgatcgtg ctgccggagc gggtggatgc cgacctgctg gcctggctgc
     6901 gcggcctgcc gtggccggtc gcagtggtgg tgcatgccaa ccatgcccat gaattcgatg
     6961 gcgccgtgga caaggcgatg gccgccctgc gggatgcagg cgccaccctg ctcaaccagg
     7021 ccgtgctgct gcgtggcgtc aacgacgcgg tggacacgct tgccgccctg tgcgaacgcg
     7081 gccacgcagc cggcgtgctc ccctattacc tgcaccagct ggaccgggtc gagggtgccg
     7141 cccatttcga ggtggacgat gcgcaggcgc tggccctcca cgccgcgctg gcggcccgcc
     7201 tgcccggtta cctggtcccg aaactcgtgc gtgaggtcgc cggcgcaccc ggcaagaccc
     7261 cgctgcaggc cgtggacgcc ggccttcccg ttgtcggatg atgtagcctt gtccctcaac
     7321 gcacggattg cagcccccgc atggcgatcg gcaaggacac cgctctacga ttgatgctcg
     7381 tcgatgacca ggtcaacgag gccgaggcca tcgtcagcag cctgcgcaac gccggcatcg
     7441 ccgtgcgccc gttgcgcgcg gaaagcctgg acgaactggc gcagatcctg tcccagcaac
     7501 cggtcgacat ggtgctggcc gaatacgcgt ccacgcagat gccgttcgcc gaagtggccg
     7561 ccgtggtgat gggctgcggc cgcgacgtac cgctgctggc ggtgctggac gggatcaccg
     7621 acgaaatcct ggacagcgtg caggcgcagg gcgcgcagac ggtggccctg cgcggccgcc
     7681 cgcagcagtt cctgaagaac gtccgcaccg aatggcacga cctcgacgcg cgtcgctcgc
     7741 agcgccacct cgaggcgcag atgcgcgaga ccgagcgccg ctgcgacatg ctgatcgact
     7801 cctcgcgcga gccgatcgcc tacatccacg aaggcatgca catccgcgcc aaccaggcct
     7861 atctggagat gttcggctac gagaccttcg aggacatcga gggaatgtcg ctgctggact
     7921 tcatcgcgcc caaggacgtg gaggagttca aggccctgct caagcggctg tccaagggcg
     7981 agccgccgcc gccgcggcac gagctcaccg cacgcgacat cgatggcacc gacttcccgg
     8041 cgatcatgga attcaccgcc gcccagtacg agggcgaggc ctgccagcag gtgatcttcc
     8101 gccgccaggc cacggaacag gatcccgaac tggcccgcca ggtggaggag ctcaagcagc
     8161 gcgacccggc caccggcctg ctcaaccggc cgaccttcct gggcctgctg gacggcgcgg
     8221 tcgccgaggc agcgggccaa ggcgccgcgc atggcctgct gctgatcgag cccgaccatt
     8281 acgcccagtt gctcgacgtg atcgggctgg atgccgccga cgacctgatg gcggcgatgg
     8341 gacaacgcct ggacagcgtc gccaacgacg ccatcaccgc ccgcttcggc gagcaccagc
     8401 tcgccctgct ggtgcgcggc agcgactacc gggccaccag cgacctggcc gaacgcgtgc
     8461 gcgaggcctt cgccggccac gtgctggagg tgggcgacaa caccctcaat gcgacggtca
     8521 gcatcggcgg cgtgcagatc ggcgagaaga tcgccagcac cagccaggtg ctggccaagg
     8581 ccacccaggg cgtggtctcc gcggtcggcg tcggcggcaa ccgggtgcaa ttgttcgatc
     8641 ccggcgcggt cgatcgcgcc gagcaggaac gcatcgcggc ctgggtcgcg cgcatccagg
     8701 gcgcattgga cagcgaaggc ttccgcctgg acttccagcc ggtcatcgcc ctgcagggcg
     8761 atggcagcga aacgtacgag atgttcctgc gcctgcacgg cagcgacggc gtcccggtcg
     8821 aggccagcac ctacctgccg atcgccgagg aacacgacct gctgcgcccg atcgaccgct
     8881 gggcggtgtc ccgcgcggtc gccctggtcg gcgaacgcct gcgcgccggc aagcgcacca
     8941 gcctgctgct gtcggtctcg caggaagcat tgctggattc cagcatgccg gccttcatcg
     9001 ccgaccagtt ggccgcagcc ggcgtgcccg gcgagcagct gatcctggcg gtgccggaag
     9061 cgaaggtgtt caccaacctg cacgcggcgc aggaattcgc cggcgcgctg gcgggcttcg
     9121 gcagccggat ggcgctggag cacttcggtg ccgggctcaa ctcgctgcag accctgcagc
     9181 atttcgagcc tgcgctactc aagatcgatc cgcagttcat ggacgacctg tccaagagtg
     9241 ccgactcgca ggcgcgcgtt cgcgaaatcg cccaacaggc gggacaacgc ggcatccgca
     9301 ccgtggcgga gcgcgtgcag gatgcgtcga gcatgaccct gctcttctcg gccggcgtgg
     9361 attatgtgca gggcgacttc ctggcccctg ccgggccgga catgacctac gacttcagct
     9421 gacccgtcct gcgcgcgccc aagaaaaagc ccgcctcccg gcgggctttt tcattctcgg
     9481 tgacgctgta accgggatcc ggtcaggcca ccacgccctg gcgcaccgca ccggcatccg
     9541 gctgcgcgtt gcgcagggcg ctgatgcgct cctccagcgg cggatggctc atcagcaggc
     9601 gcttcaggcc gttgcccaca acgccgctga tgccgaacgc cgcgacctgc ttgggcaggc
     9661 tgctatgccc ataggtctgc gacaagcgct ccagcgcggc gatcatcttc tgcttgccgg
     9721 ccagcgcggc accgccagca tcggcgcgga actcgcggtg gcgcgagaac cacatcgcga
     9781 tcatcgaggc gaacaggccg aacaccatgt ccagcacgaa cacgcccagg taatagccga
     9841 tgccgtatcc atcgcgattg cccaggtagc catcgatcgc gcgaccgatc acccgcgaca
     9901 gcacgatcac gaaggtgttg agcacaccct gcagcagcgc catcgtcacc atgtcgccgt
     9961 tggcgacgtg gctgacctcg tgggcgagca ccgcttcggc ctcgtcgcga ctcatcgccc
    10021 gcagcagccc ggtggacacc gccaccagcg cgttgttgcg gttggcaccg gtggcgaagg
    10081 cattgatttc cggggcgtcg tagatcgcga cctcgggcat gccgatgccg gcagcctggg
    10141 cctggcggcg caccgtaccc aacagccatt gttcggcctc gtttcgcggg gtttcgatga
    10201 catgcgcgcc ggtcgcgcgc ttagccatcc atttggacac cagcagcgag atgatcgagc
    10261 cgccgaagcc gaacagcgcc gccatgatca gcaggccgcc aaactgccgt gggttcaccc
    10321 cgaggatcga catcacgata ctgaccaggg ccagcaccgc caggttggtt gcaagaaaga
    10381 ggaatccgcg cttgaacatc gatctgccat cctgccgcag ggggagcgcg gcttggtccg
    10441 gaagtgtggc cacgcgcctg taaattcaat ctgaagacga ccgacccacg atgcccctgc
    10501 ccgcctaccg cggccgtttc gcgccctctc ccaccggtcc gctgcatgcg ggctcgctgc
    10561 tggccgccct gggcagctgg ctgctggcgc ggcaggccgg cggtgaatgg ctcctgcgga
    10621 tcgaggatgt ggatccaccc cgcgaaatcg ccggcgcggc cgacggacag cgcgccgcac
    10681 tggccacctt cgggttgcac cacgacggcc cggtcatcca tcaaagcaag cgcgggggga
    10741 tttaccagga ggcactcgat cggttgctgg cgaaggggct cgccttcgaa tgccactgca
    10801 gccgcagcga cctggtcgcg agcggcggta tccaccgcca ctgcgtggcc gctgcacgac
    10861 ggccggaccc ggcgatccgc ctgcgcgtcc cggacgagtg cgagatcggc ttcgatgacc
    10921 tggtgcatgg ccgcgtggtg caacgcgtcg atgtcgatgc cggcgacttc gtgctgcgcc
    10981 gcgccgatgg cccgtgggcc taccagctcg ccgtggtcgt ggacgacgcc gcgcagggca
    11041 tcaccgaagt ggtccgtggc gcagacctgc tggactccac cccgcgccag atcctgctgc
    11101 agcgcgcgtt ggacttgccg acgccgggtt acgcgcacct gccgctgttg ctcgacaccg
    11161 acggcagaaa gttgtcgaag tcctgcgcgg cgatgccggt cgatcccgac gatccgatac
    11221 cggccttgca cgcgtgctgg ttggcgctcg ggcaaggcgc gctgccggcg acgatcgact
    11281 cgaccgaacg attcctggcg gcggcgatcg aggcgttccg catcgatcgg gtgccgcgcg
    11341 acgcgttgcc cgcatccgcc gcagcgcaca acaccgatgc aacatcgcgc cgctagaatc
    11401 cctcttcaca tctgccactt cccaggagtt cccccatgac ttcccgcgtc gcactcgtca
    11461 ccggtggcac cggcggcatc ggcaccgcca tcgtcaagcg gttggccgac atgggccaca
    11521 aggtcgccac caactaccgc aacgaggaga aagcacgcgc ctggcaggag cagatgaagg
    11581 cggccggcta cgacatcgcc atcgtcaagg gcgacgtgac ctcgccacag gaagcggaag
    11641 cgatggtccg cgaagtcgag gccacgctcg gcccggtcga catcctcgtc aacaacgccg
    11701 gcatcacccg cgacggcacc ttccacaaga tgaaggccga ccaatggggc gacgtgatca
    11761 ataccaacct caattcggtg ttcaacgtca cccgtccggt gatcgagggg atgcgcgagc
    11821 gcaagtgggg ccgggtcatc cagatcagtt cgatcaatgg cctgaagggc cagtacggcc
    11881 aggccaacta cgccgcggcc aaggccggca tgcacggctt caccatctcg ctggcgcgcg
    11941 agaacgcccg caatggcgtc accgtcaaca ccatttcgcc gggctacatc gccaccgaca
    12001 tggtgatggc ggtgccggaa gaagtgcgcg cgaagatcgt cgccgacatt cccaccggtc
    12061 gcttgggcac gccggaagag atcgcgtatg gcgtgggctt cctcgccgac gagcaggcgg
    12121 gctggatcac cggctccaac ctcgacatca acggcggcca ccacatgggt tggtgaggcg
    12181 aatcttgggc atggcccaag atcccccgaa gttcgctacg cgaacttcgg gccccgccac
    12241 gtacatcgca cacccccatt cgagcccatg gcgcgaatcg cccccttggc tcaggggctg
    12301 ggcactgctg gtgaccgttg catcgcggat atgccaccat cgccctgatc cactcccagc
    12361 cagccatgcc acggagcccg catgtccaac gtcgatccga accgccgtcc ctcgaatgcg
    12421 ggccgttatg ccttcctgct gatcctcggc ctggtgctgg gcgtgatcgc cacggtgatg
    12481 acgatgcgcg cattgaacga acgcaaggat cacttccctg atgcgctgat gcacgtgcag
    12541 caatggcaca tgggacagtt gaaggccgac atggacgcga accgctgcaa cgcgaccgac
    12601 atcctcccgc acctgcaggc gctgcgggtc accgcgaacg acatcgacgc cgcgttcccg
    12661 gacctgcgcg acgacacccg cttcactgcg gcaagttcgg cgatgcgcgc cgccaccgac
    12721 aaggcgatcg ccaatccgcc gctgacctgc gacggcctgg gcgcgacgat gaaatcgatc
    12781 ggcgaaacct gcaaggcctg ccaccaggat ttccgcggct gagcgctgcg cacaggcatt
    12841 cgacaaggcc gcctccgggc ggccttgttg tttcagagcg cccggtccgc gttgacggcg
    12901 cctccacctc ggtgaatcac ccgtaagccg gaacctcccg aaacatcgaa tgtcgggttc
    12961 cgttcatgcg cgcagtcaca ggatcgcccc gtcgtccaag tcggcgaccc atcgttccac
    13021 gcaaatcatt cgctccatcg gaaggagact ccacatgacg atccgcactc cacacatccg
    13081 caccttgggc gcggccatcc tcggcctggc cttggccgca ggcacgggct gcgccagcac
    13141 cggttcgtcc ggctattccg gcggaccgag ctacggcggc aactacggca acacctgcca
    13201 gaactgcggc accgtcaccc gcatcaccgt gggcggcgag tcaggcaagg ccggcgcagt
    13261 gatcggcggc atcatcggtg ccgttgcagg ccacgagatt tccgcgcaga ccggcggcag
    13321 caagggcaac cagaacatct cggccgtcgc aggcgccgct gcgggcgccg ctgcaggcaa
    13381 tgccatccag aagaatcgca gcgatggtta caccgtgcat gtccgcatgg acgacggccg
    13441 caccaccgcc gtgacccagg acaacgtgtc cggcttccgc gaaggttcgc gggtccgcat
    13501 ttcggatggt cgcgcttacc tgcagtaacc ccccacagcg cccgtcacgc cacacgcgaa
    13561 cggcccgcag tgcgggccgt tcgcgtgcct gaccacgcat tcctgcagaa tcgtccccgc
    13621 gccttcaccg gataccccat ggaaccgacc accctgtact acgtgcttgc cgccctgctg
    13681 gtcgtggtgg gcatcgcggg gaccatcctt ccggccctgc ccggcctgcc gttggtattc
    13741 gcggggatgc tgctggccgc ctgggccggc aatttccagc aggtaggcgt tccgatgctg
    13801 gtggtgcttg gcctgttgac ggtgttctcg ctggcggtgg atttctgggc gaccgcgctc
    13861 ggcgccaagc gcgtcggcgc gagccggttg gcgatcatcg gagcgatggt cggcaccttt
    13921 ggcgggctgt tcctggggcc actcggcctg ctgatgggcc cgttcgcagg cgcgatcggt
    13981 ggtgagctga tccatcggcg cagcctgctg aagcaggatc tcgggcatgc caccaggatc
    14041 ggcgtcggta cctggttcgg catcctgttc ggcaccgtgc tcaagctggc gctggccttc
    14101 accatgctgg gcctgttcgc gctggcctgg tggctgtagc cacagggctt tcacgccacg
    14161 ctgaacgcgt tcaggccaag ctgcgcgccc actggatccc gcaccggatc gccgcgatgc
    14221 cacgacgctg gagccgcaaa cgcatggcct ggacgattgc cattaccgcc ctcgtgaccc
    14281 tgctattggt caccctggcg atgaacttca agacatcgga aaaaaccctg gaacgcaaga
    14341 tcgagcatcg ctacgcggtg tccgacccgc agttccggcg cgagatgggc gtcctgctgg
    14401 gccccggcat catccagggc aaccgggtca ccgacctcga gaacggcgac gagatcttcc
    14461 cggcgatgct ggaagcgatc cgcggcgcca ccaccacgat cacgttcgag acctacatct
    14521 actggtccgg cgacatcggc gcgaaattcg ccgatgcact cgccgagcgc gcacgtgccg
    14581 gcgtcaaggt caaggtgatg gtcgactggg tcggcagcat caagatggag gacgacctgc
    14641 tgcagcgcat gcaggacgcc ggcgtggaga tccacaagta ccggccgctg aagtggtaca
    14701 acctcggccg cctcaacaac cgcacccacc gcaagctgct ggtgatcgac ggccgcatcg
    14761 gtttcaccgg tggcgtgggc atcgccgacc agtgggaagg ccacgcgcag gacaccgacc
    14821 actggcgcga cctgcacttc cgcatcgagg gcccggtggt ggcgcaggtg cagtcggcgt
    14881 tcaacgacaa ctggatcaag accaccggcg tggtgctcaa cggcgccgat tatttcccgc
    14941 cgctggcgac cgtgggcgag atggacgcac acatgttcgt ggcctcgccc gctggcggca
    15001 gcgagagcat gcacctgatg tacctgatgg cgatcgccgc cgccgagcgc agcatcgacc
    15061 tggaggccgc ctacttcgtg cccgacccgc tgatgatcca ggccctgcag gccgcgcgtc
    15121 accgcggcgt tcgggtgcgg gtgatcgtgc ccggcaagca catcgattcc gacaccgtgc
    15181 ggctggcgtc gaaggcgcac tggggcgagc tgttgctggc cggggttgag atccacgaat
    15241 accagcccac gatgatgcac aacaagctgc tgatcgtgga cgggatgatg acatcggtgg
    15301 ggtcgaccaa cttcgacatc cgctcgttcc ggctcaacga cgaagccagc ctcaacgtgt
    15361 acgacgccgc gttcgcccag cgcatgaccg aggtgttcga gggcgacctc aagccgaccg
    15421 tgcgctacga ctaccggatg tggaaaaatc gctcgctgaa ggaaaagctg ttcgagaagt
    15481 tcatccttcc gttcaagtcc caactctgac gcgagccgat gccgatcctg gaccccgaca
    15541 agctccccac cgcaagcagc ctcggcgaac gcttcatgca ggcgctggaa caggtccagg
    15601 acaagttgct gcacctggtg gccagcgccc cgctgctgct ggtcgccctg ctgatcgtac
    15661 tgttcgccag ctggctgggc ggcttcgcca gccggcacct gcgggtggtg aaacgcttca
    15721 gcagcagcaa cccgtacatg gagggcctgc tgcgcagcac ggtgcggatc gtcatcacct
    15781 tggccggcgt ggtggtcgcg ctggacctgc tcaacgcgac gtccctggtc ggtgcggtgc
    15841 tgggttcggc cggcgtgatc gggctggtac tcggcttcgg cttcaaggac atcgcggaaa
    15901 actacatcgc cggcgtgctc ctcagcctgc gcaagccgtt ttctccgggt gacaccgtgc
    15961 ggatcgacag cttcgagggc aaggtggtgg cgctgacggc gcgtgccacc atcctgatga
    16021 cggtggatgg caatcacctg caattgccca atagcgtggt gttcaagtcg gtactgctca
    16081 actacagccg caatccgaag cgccgcttcg acttcgagac cgtggtcgac aaccgtgcct
    16141 cctggcacgc ggcgatggac gcggggcttg ccgcgatcgg ctcggtggag ggcgtgctcg
    16201 ccgagccacc cccgtcaacg ctgatccgaa ccctttccaa cgatggcgcg acattgcagt
    16261 tcgccggctg gatcgaccag cgccacaacg acctggccaa gacccgcagc gaagcgatgc
    16321 gacgggtccg ccgcgagctg cgcgaagccg gcttcgtgcc gccggaacac gtgcagaagg
    16381 tgatcctgca acgtggcgag gccgacttcc cccaggccca ggagaacggg catgcacggg
    16441 atacctcggt cgatcgcgcc ctcgacgccc aggtcggcaa tgcgcggcgg ctggaggacg
    16501 gcggcgacct gctgcgaccg cccccgccgg gttcgccacc gacctgaatc agccgccgta
    16561 caacagccag tgccgtgctt cgttctcgtt ggcgaagatg cggatggtga tcccgcgctc
    16621 ctgcagcatg atctcgccaa gttcgctgcc ctggatgtcg tcgcgcagtt ccacgaaggc
    16681 actgcggaag cggcccatgc cggcggcctc gatggcgtcg atcaccttgg caaggtcgtc
    16741 cagtccgacc gtcgacacca ggtcctcgag gatcagcatc cgcgtcgcac cgaccgcacc
    16801 gccctcggcg gcgagcatcc gccacatcgc gatcgagata tccaccgagt cggtgccgtc
    16861 gaacacgtag gcgcgcaggt agcccggctc gtcctcgtag cagaccttga accccggccc
    16921 gttgacgacg cgggtattgc tcatgcgacc accaccggga tccccccgat catgcccttg
    16981 gcgatccgcg cctgccattc cttcgggccc gtctggtgca ccgaggtgcc ttgcgaatcg
    17041 accgcgacgg tgaccggcat gtccttgacc tcgaattcgt agatcgcctc catgcccagg
    17101 tcggcgaagc cgaccacgcg cgcggccttg atcgccttcg acaccaggta ggccgagccg
    17161 ccgaccgcca tcaggtacac cgattggtgc ttcctgatcg cctccagcgc gaccgggccg
    17221 cgctcggcct tgccgatcat gcccagcagg ccggtctgcg ccagcacctg ttcggtgaac
    17281 ttgtccatgc gggtggcggt ggtcgggccc gcgggaccca ccacttcgtc gcgcaccgga
    17341 tcgaccgggc cgacgtagta gatgaagcgt cctgccaggt cgaccggtag cggctcgccc
    17401 ttgtcgagca tctcgacgat gcgcttgtgg gcggcatcgc ggccggtgag cattttcccg
    17461 ttgagcagga gcacctcgcc gggcttgaac gtcagcacgt cctcgcgggt gatgcggtcc
    17521 aggtccacgc gccgtgcatt ctgcgggttg taggtcagcg tcggccaatc ctccagcgac
    17581 ggcggctcca gcgcgaccgg gccggtcccg tcgagggtga aatgcgcgtg ccgggtggcc
    17641 gcgcaattcg ggatcatcgc caccggcagg ttcgcggcgt gggtcgggta atccttgatc
    17701 ttgacgtcca gcaccgtggt caggccgccc agcccctgcg cgccgatacc gagcgcgttg
    17761 accttttcgt acagctcgat gcgcagctcc tcgaccctgt tcgacgggcc acgctcgatc
    17821 aggtcctgga tgtcgatgtg ctccatcagc gattccttgg ccagcagcat cgccttctcc
    17881 gcggtgccgc cgatgccgat gccgagcatg cccggcgggc accagccggc gcccatcg
//
LOCUS       DAOOCX010000044        17382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_2916_length_17382_cov_8.922722, whole genome shotgun sequence.
ACCESSION   DAOOCX010000044 DAOOCX010000000
VERSION     DAOOCX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 17382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17382
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2916_length_17382_cov_8.922722"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1379
                     /locus_tag="PK399_05980"
     CDS             174..1379
                     /locus_tag="PK399_05980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW12700.1"
                     /translation="MDLKSGYPFWAVKNGLMHAFPPLQEDLRCDVAIIGGGITGALVA
                     DELAAHGHDVAVVDQRDIGWGSTSASTALLQYEIDTHMVDLAKRYGEDNAVLAYRACA
                     DAIDLVAAKAGDIRDVDFARMRSLYYASKRRHRRDLEDEFALRKRHGFEVEWLDGGRV
                     KADYGFDAPGAILSAKAARIDPYRMAYRLLARLQKRGGAVHDRTPIARIEVDDRGATL
                     TSNDGATIKAKHLVMAAGYASQQFLRKKVAKNRSSYAFITDPIDAGTLGALKDTMVWE
                     SARPYLYMRTTGDGRLLVGGDDDSVDIPARRDAQVDRKARKLADKVAELFPDVPLEPA
                     FAWAGTFAETDDGLPWIGVSPDHGRRLLFAMAYGGNGITYSMLGAGLIRALVERRKHP
                     LQGIFGFER"
     gene            1452..3131
                     /locus_tag="PK399_05985"
     CDS             1452..3131
                     /locus_tag="PK399_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HPW12701.1"
                     /translation="MTEATPTGVARTAQIVRFLFKYRNAGVFSGLEVDAAGLDTGEHA
                     DEGRPEAFVDDLEAMGPTFIKIGQALSTRPDMVPPAYLVALERMQDDVAPAPFADIRA
                     VIEEELGVKLGKLFESFEETPIGGASLAQVHRARLRDGRAVAVKVQRPGISDGIHADL
                     DALAALARQVDRHTEVGRRTRFADWVHEFRRSLLAELDYRTEAGNLARFGAHFERYPR
                     LFVPQPVWDLSARRVLTMELVEGAKATDIGGLRRTEQDMAPLAEAVMRGYLDQAFVHG
                     EIHADPHPGNLLITPDGRVALLDLGMVAHVPPRQRERLLKLLFAAVDGRGEEVANEAI
                     AMSIQLQDFDEERYVREVGQMVARYAATSGNRSQSEGRLVLDLTLLGSQCGLRTPPEL
                     SLLGKTLLNLEAVSKALDPDMDVKSVVEQHLEHVMRERLKKSFSPARLASEAMEVQAL
                     VREAPRRLSDILGLLAENRLQVRMSGLDDSQLMENVQKIANRISTGIIVAALILASAL
                     LMRIDTGPRLLGYPALALVLFVVGAVLGLAIVASALLNDRKAKPQETHGPR"
     gene            3189..3905
                     /gene="pyrH"
                     /locus_tag="PK399_05990"
     CDS             3189..3905
                     /gene="pyrH"
                     /locus_tag="PK399_05990"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010366166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPW12702.1"
                     /translation="MSQLAYRRVLLKLSGEALMGDEDYGIDPKILNRLAREVIEVRDA
                     GVEIALVVGGGNIFRGAGLAAGGMDRVTGDQMGMLATVINALAMQDALEKLGAKCRVM
                     SALKINDVCEDYIRRRAMRHLEKGRIAIFAAGTGNPFFTTDSGAALRAIEIGADLLLK
                     ATKVDGVYDKDPNRHADAVKLDDLRYDDVIARNLQVMDTAAFALCRDAGLPLRIFDMA
                     QPGVLLRILRGEKIGTLVHA"
     gene            3990..4547
                     /gene="frr"
                     /locus_tag="PK399_05995"
     CDS             3990..4547
                     /gene="frr"
                     /locus_tag="PK399_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPW12703.1"
                     /translation="MLNEIKKDAQARMGKSIESLRHALVKVRTGRANAGLVDSIKVSY
                     YGSEMPLAQVASVAVTDARSITITPWEKQMVGPVEKAILASDLGLTPNTAGTVIRLNI
                     PALTEERRRDLAKVVHGEGEDAKVAIRNIRRDANHQVKELLKDKQITEDDASRTEAEI
                     QKITDGAIKDVDEVIKGKEAELMSV"
     gene            4675..5433
                     /gene="uppS"
                     /locus_tag="PK399_06000"
     CDS             4675..5433
                     /gene="uppS"
                     /locus_tag="PK399_06000"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPW12704.1"
                     /translation="MSGANGTGPIPQHLAIIMDGNGRWAQQRHRPRIIGHRAGARAVK
                     RSVEFCLERGVGALTLFAFSSENWNRPTEEVGGLMKLFLGALEREVDELHRLGARLRF
                     IGERSRFDGPILARMQAAEALTAGNTRLQLNIAASYGGRQDIAQAARALAEEVAAGRL
                     RPEQIDEDALSARMALADLPAPDLFIRTGGELRISNFLLWQLAYTELWFTDTLWPDVD
                     AATLQRALDHYAGRERRFGLTSAQVAGHATGGIE"
     gene            5430..6281
                     /locus_tag="PK399_06005"
     CDS             5430..6281
                     /locus_tag="PK399_06005"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011258695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPW12705.1"
                     /translation="MTKTRVLAALIMAPLAIGGVLLLPTPWLVAIAAVLFLLALWEWL
                     RLADVEDTLARTILLACNLALMVALVWGSRSAHGGSFALFQLVIVIGVGWWLLALLWL
                     KHYHFASDHDSHARAFKLLAGTLAVVPAWCALGVIHASEPNGHYWLLLALMLVWAADT
                     GAYFTGRNFGGKWFGGRKLAPRISPNKTIEGLLGGIALAMLVAMVGALLIGVDQAHLP
                     AVAGVAAVTVAFSVVGDLYESLLKRHVDIKDSGHLIPGHGGVLDRVDSVLAALPVFAL
                     GKVWFGF"
     gene            6278..7462
                     /gene="dxr"
                     /locus_tag="PK399_06010"
     CDS             6278..7462
                     /gene="dxr"
                     /locus_tag="PK399_06010"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPW12706.1"
                     /translation="MSGSSMQRVAILGATGSIGASALDVIARHPDRMRASLLAAGTRV
                     DELLALCRVHRPEHAVIADAGAYAALREGLRDAGLDTQAHAGAGALDALVAGDACDSV
                     VAAIVGAAGLPSTLAAARAGKRLLLANKESLVLAGELLMQAASANGAVIVPIDSEHNA
                     IFQCLAGNPGEMPARITLTASGGPFRGWTREALATVTPAQAVAHPKWSMGPKISVDSA
                     SLMNKGLEVIEAHHLFGMSGERIRVLVHPQSLVHSFVDFADGSTLAQLGLPDMRTALA
                     VGLGWPRRIASGVPALDLLAEGGLLEFAPPDLDAFPCLALAFRALQAGDAAPAVLNAA
                     NEEAVSAFLQGRIGFLSIPDVVAHALDAMPATPADSIDALLDADAWARAQACAFIARK
                     AA"
     gene            7487..8845
                     /gene="rseP"
                     /locus_tag="PK399_06015"
     CDS             7487..8845
                     /gene="rseP"
                     /locus_tag="PK399_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPW12707.1"
                     /translation="MSTFLGSLWWMLVALGVLVTFHEFGHFWVARRCGVKVLRFSIGF
                     GRPLWTRHGKDGTEYVVAALPLGGYVRMLDEREDHVPAHQLDQAFNRKPVLQRMAIVA
                     AGPAANLLLAFALLWTMLVIGRPDYAPVIGKATGIAAQAGLRTGDRILAVGERETPTW
                     SEAGMALTVAVLDRQPVQVRVQGGDGEESTRTLALDRLPASIDELRALEATGLIARHQ
                     LWPAIVGSVRADTPAWGVLAEGDRITAIDATPIASFDDIPPAVAALGTRGGPGMVEIE
                     RDGERLALELTPVRLVVGPGSSAWALGVAPAERRMPATDAVLRVGPLDAIPAAVRETG
                     FQMRQLVEMVGRAFAGKVSVQNTVAGPLTIAQAANDFASRGAAWFLNFLALLSVSLAL
                     INLLPIPVLDGGHLLYYLIELARGRPLGERAMAAGQYLGLALVAGLMGLAFYNDILHL
                     VS"
     gene            8913..>11248
                     /gene="bamA"
                     /locus_tag="PK399_06020"
     CDS             8913..>11248
                     /gene="bamA"
                     /locus_tag="PK399_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPW12708.1"
                     /translation="MTRPTSRRLLALALATALSMPAWAQTAFAPFSVSDIRIDGLQRI
                     GAGTVFTYLPVERGDTLDQAKAAEALRALYKTGFFEDVRLDHQGSILVITVVERPAIN
                     KLTLVGNKDLKTEDLLKGLKDIGLAEGETFNQLNLDRVTQELNRQYNNRGKYSVSISP
                     NVEKLDRNRVNLTLNIKEGKAAKIRHINLIGNESFPEEDITKGWESHTSNWLSWYKRD
                     DQYSREKLSGDIEKLNAWYLDRGYVDFSLDSTQVAISGDRKDMYLTAGMTEGEVYSVS
                     TVTVSGETVLPKEEIETIVAFIRPGSTFSRGLLELATDAISARLSNIGYAFARINPVP
                     QIDREKRTVGIDFQVQPGPRVNVRRIVFKGNTRTADQVLRREMRQFEGTWYSQAAIDR
                     SKVRLDRLGYFEEVNVENEPVAGTDDQVDVVYTVKETTSGSIAAGVGYSQLSGVNLSL
                     QLSENNFLGTGNRVSMSVTRSAYQKRYDFSFMNPYFTDEGLSLGYNLWWREFDYSSFN
                     VASYSTNSGAAQVFLGLPLSESDSVSLMFGIDSNEILAFPGSTPPPLVDYISAVGNQT
                     FHSWRAQVGWGRDTRNNYFTPVVGTTQNLSAEIALPGSTVEYFKVAYDFAKYWPLSQA
                     LVLKTGVNLGYGDSYGKDFTRNLCYTAPTYTDTNNDGIPDTVVPGPAPTDPCLPTSPD
                     FDKTVTASGLPFFENFYAGGISSSGKVRGFTDNTLGPFYQTASGFRQPLGGSFLVAGS
                     VEAIFPKLFDSPAARISAFVDFGNVYNGFDNFDAGELRA"
     assembly_gap    11249..11348
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11349..12044
                     /gene="lpxD"
                     /locus_tag="PK399_06025"
     CDS             <11349..12044
                     /gene="lpxD"
                     /locus_tag="PK399_06025"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007965614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPW12709.1"
                     /translation="VVSPGAHIGPFTSIGARTTIGAGVVVGPGCVIGDDCEIGEGSEL
                     QARVTLVTRVRLGKRVRILPGAVLGAAGFGLAMEHGRWLNIPQLGGVVVGDDCEIGAN
                     STIDRGALGDTVLEEDVRLDNQIQVGHNVRIGAHTAMAGCSAVAGSARIGRYCLVGGG
                     AGVLGHLEVCDRVVITAMSLVTHSLREPGEYSSGTPLMDNRSWRKSAARFKQLDRMAR
                     ANPRAGAGDKDQE"
     gene            12041..12508
                     /gene="fabZ"
                     /locus_tag="PK399_06030"
     CDS             12041..12508
                     /gene="fabZ"
                     /locus_tag="PK399_06030"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPW12710.1"
                     /translation="MTETPFRVTMPVNTPEIERLLPHRYPFLLVDRVIEFEKDKRVLA
                     YKNVTCNEPFFTGHFPGRPVMPGVLVVEALAQAGGLLTQLSRDPAEIGKDTFYLVKVD
                     NARFSRMVVPGDRLELEVELKRRIRNMAQYVGIARVDGEQAACAEILCAAARE"
     gene            12505..13299
                     /gene="lpxA"
                     /locus_tag="PK399_06035"
     CDS             12505..13299
                     /gene="lpxA"
                     /locus_tag="PK399_06035"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPW12711.1"
                     /translation="MSNATVLVHPTAAIDPDARLGAGVHVGAYAVIGAGVEIGDGCRI
                     GPHCTIEGPTRIGRDNVFHAHAAIGGEPQDKKYRGERVELVIGDGNVIREFVTINRGT
                     GDGGGITRIGDRNWILAYCHVAHDCIIGNDCVFSNNATLAGHVTVGNHVILSGFAGVH
                     QFCRIGDHAFIGMGAFVNGDVPPFLMVAQEKYARPRGINAEGLKRRGFDAGRIASIKR
                     AYRALYMGDAKLDEAKSELGEIAASGSDDVRAMLDFIERGERPLLR"
     gene            13418..14560
                     /gene="lpxB"
                     /locus_tag="PK399_06040"
     CDS             13418..14560
                     /gene="lpxB"
                     /locus_tag="PK399_06040"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPW12712.1"
                     /translation="MSLRIALCAGETSGDQLGAGLIEALRAHFPDAEFAGIGGDAMRA
                     AGMDTWFDASELAVMGLAEVLAHLPRLLKLRRTFRQRVLDWQPDVFIGIDAPDFNLGV
                     ERWLKQRGVRTVHDVSPSVWAWRESRAAKIAASADRVLCLFPMEPPIYARHGVDAVFI
                     GHPLADAIPLQPDRAAARHALGESRDGPILALLPGSRLGEIQRMLPTFLEAARLLHPD
                     VPGLRLLIPAANAQCRAAIDAIVGDAPAMRVLDGNAQRAMIASDVVLLASGTAALEAM
                     LCKRPMVIGHRISGLTYAIVKAFGLLKSAHVSLPNVLAGETLVPELLQDDCTPANLQA
                     ALLRWFCDADAVEALQPRFLAIHETLRRDASARAADAVAGLVGTPA"
     gene            14557..15171
                     /locus_tag="PK399_06045"
     CDS             14557..15171
                     /locus_tag="PK399_06045"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPW12713.1"
                     /translation="MIQRIAGIDEAGRGPLAGPVVVAAVVFPPGRTPVNGLDDSKALT
                     ARKREALYPRIIERALAWKIVFIAPAEIDRRNIYHATLHGMREALLGVAHMADVARID
                     GNRLPPDLPCPAEAWIGGDARDRTIMAASILAKVARDQAMRELHLLYPHYGFDQHKGY
                     PSPSHLQALREHGPCPHHRRSFAPVRDCGGTPMQGDLLAQTAPC"
     gene            15255..>17382
                     /gene="dnaE"
                     /locus_tag="PK399_06050"
     CDS             15255..>17382
                     /gene="dnaE"
                     /locus_tag="PK399_06050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPW12714.1"
                     /translation="MPARFVPLHLHSEYSLVDSTIRIPELVAACAARGLPAVAITDQN
                     NLFALVKFYKEAEKAGIKPIAGADVLVAEGTAAPSRLTLLCRDNDGYLSLSQILTRAW
                     MEGHRVDGVVARPAWLAENAGLFAIAGPQSEPGQLFASGRHDLAEQALADLQAKFGDR
                     LHLELLRTGRAGEDAFNAFALDVSARRSIPLLASNDVRFLDADGFDAHEARVCIASGR
                     VLDDTRRPHDYSPQQYLKSADEMAALFIADAPDALDNTVALAQRCNLGLRLGTYYLPA
                     FPVPSDETLDSWIRSQSREGLVKRLEKAPLATGKTRADYEARLELELDVIVKMGFPGY
                     FLIVADFINWAKQHGIPVGPGRGSGAGSLVAWALGITDLDPLPYDLLFERFLNPERVS
                     MPDFDIDFCMDRRDEVIDYVARKYGRDRVSQIITYGTMAAKAVLRDAGRVLGFPYGFV
                     DGLAKLIPLQPTDPMTLEDAIGIGKRAQKEPDRVVTEFRDRYHAEDDVRDLVDLALQL
                     EDLTRNAGKHAGGVVIAPSPLQDFCPLFAEHDGHGLGRNPVTQFDKDDVESIGLVKFD
                     FLGLRTLTIIDWAVKAINVRRAAAGESAFDIMSLPLDDKATYELFARGDSVAVFQFES
                     RGMRELLKRAKPDTFEDIIALAALFRPGPLGSGMDKQWCDRKHGIEDVSYPHPALEQV
                     LAPTYGVIVYQEQVMQIAQVLAGYSLG"
BASE COUNT         2788 a         6010 c         5814 g         2670 t
ORIGIN      
        1 ggtcgtcgag gactacgccg cggaagtggc gaaggccatg caggtctgat cgacctcgcc
       61 gccgcgtcat cgaagaaccc gccgcaaggc gggttttttg ttgcgtggcg cgtgacggac
      121 gcatcacgcc accgccacca gaatcgtcgc atcgctggca acggaacgac acgatggacc
      181 tcaagagcgg ttacccgttc tgggcagtga agaacggcct gatgcatgcg ttcccgccgc
      241 tgcaggagga cctccgctgc gacgtggcga tcatcggcgg cggcatcacc ggcgcgctgg
      301 tcgcggatga gctcgccgcg cacgggcacg acgtcgccgt ggtcgaccag cgcgacatcg
      361 gctggggcag cacgtcggcg agcaccgcgt tgttgcagta cgaaatcgac acccacatgg
      421 tcgacctggc gaagcgctat ggcgaggaca acgcggtcct cgcctaccgg gcctgcgccg
      481 atgcgatcga cctggtcgcg gccaaggccg gcgacatccg cgacgtggat ttcgcccgca
      541 tgcgcagcct gtactacgcc agcaagcgtc gccatcgccg cgacctcgaa gacgaattcg
      601 cgctgcgcaa gcgccacggc ttcgaggtcg aatggctgga cggcgggcgc gtgaaggcgg
      661 actacggatt cgacgcgccc ggcgccatcc tgagtgccaa ggcggcgcgc atcgatccct
      721 atcgaatggc gtaccggctg ttggcccgat tgcagaagcg cggcggcgcc gtgcacgacc
      781 gcacgccgat cgcgcggatc gaagtggatg atcgcggcgc gacgctgacc agcaacgacg
      841 gcgcgacgat caaggcgaaa cacctggtca tggcggcggg ctatgccagc cagcagttcc
      901 tgcgcaagaa ggtggcgaag aaccgcagca gctacgcgtt catcaccgac cccatcgacg
      961 cgggcaccct cggtgcgctc aaggacacga tggtctggga atccgcacgc ccctacctgt
     1021 acatgcgaac caccggcgac ggccgcctgc tggtcggcgg cgacgacgac agcgtcgaca
     1081 tccccgccag gcgcgacgca caggtcgatc gcaaggcgcg caagctcgcg gacaaagtcg
     1141 ccgagttgtt ccccgacgtt cccctcgaac ccgcattcgc ctgggccggc accttcgccg
     1201 agaccgacga cggcctgccg tggatcggcg tgtcgccgga ccacgggcgg cgcctgctgt
     1261 tcgcgatggc ctacggcggc aacggcatca cttactcgat gctcggggcc gggctgatcc
     1321 gtgcgctggt ggagcggcgc aagcatccgc tgcagggcat cttcgggttc gagcgctgag
     1381 cggcgcgtgc gcgccatgct cacgtgctgc agcccaggct gcgcatcctc tttcgcattc
     1441 ccggtgacga catgaccgaa gccactccca ctggggtcgc caggaccgcg cagatcgtgc
     1501 gattcctgtt caagtaccgc aacgccgggg tgttctccgg gctggaggtc gatgccgccg
     1561 ggctcgacac cggggagcac gcggacgagg gacggcccga ggccttcgtc gatgatctcg
     1621 aagcgatggg gccgaccttc atcaagatcg ggcaggccct gtcgacccgc ccggacatgg
     1681 tgccgccggc ctacctggtc gcactggagc gcatgcagga tgacgtcgcg cccgcgccgt
     1741 tcgccgacat ccgggcggtg atcgaggaag agctgggagt caaactgggc aagctcttcg
     1801 agagtttcga ggaaaccccg atcggcggcg cctcgctggc ccaggtccac cgcgcccgcc
     1861 tgcgcgacgg acgcgcggtc gcggtgaagg tgcaacggcc cggcatcagc gacggcatcc
     1921 acgccgacct cgacgcgctg gccgcgctgg cgcgccaggt cgaccggcac accgaggtgg
     1981 gccggcgcac ccggttcgcc gactgggtgc acgaattccg ccgcagcctg ctggcggaac
     2041 tcgattaccg caccgaggcc ggcaacctgg cccgcttcgg cgcgcacttc gaacgctacc
     2101 cgcgcctgtt cgtgccgcag ccggtctggg acctgtcggc gcgccgggtg ctgaccatgg
     2161 aactggtgga gggcgccaag gccaccgaca tcggcggctt gcgccgcacc gagcaggaca
     2221 tggcgccgct ggcagaggcg gtcatgcgcg gctacctgga ccaggcgttc gtgcatggcg
     2281 agatccatgc cgacccgcat ccgggaaacc tgctgatcac cccggatggc cgcgtcgccc
     2341 tgctcgacct gggcatggtc gcccacgtgc cgccacgcca gcgcgagcgc ctgctcaagc
     2401 tgctgttcgc cgcagtcgac ggacgcggcg aggaagtggc caacgaagcc atcgcgatga
     2461 gcatccagtt gcaggacttc gatgaggaac gctacgtgcg agaggtcggg cagatggtcg
     2521 cgcgctacgc ggccacctcc ggcaaccgat cgcagtcgga agggcggctg gtgctcgacc
     2581 tgaccctgct cggcagccag tgcggcctgc gcacgccgcc ggaactcagc ctgctgggca
     2641 agaccctgct caacctggag gccgtgagca aggcgctcga cccggacatg gacgtcaagt
     2701 cggtggtcga gcagcacctc gagcacgtga tgcgcgagcg cctgaagaag tcgttctcgc
     2761 ccgcgcgcct ggccagcgag gcgatggagg tgcaggccct ggtgcgggag gcaccgcgcc
     2821 ggttgtccga catcctgggc ctgctcgcgg agaaccgcct gcaggtgcgg atgagcgggc
     2881 tggacgactc gcaactgatg gaaaacgtgc agaagatcgc caaccgcatc agcaccggga
     2941 tcatcgtggc cgccctgatc ctggcctcgg cgctgttgat gcggatcgat accggaccgc
     3001 gcctgctggg ctatcccgcg ctggcgctgg tgctgttcgt ggtcggcgcg gtgctcggcc
     3061 tggcgatcgt cgccagcgcc ctgctgaacg accgcaaggc caagccgcag gagacccacg
     3121 gcccgcgctg agggacgcat cggctagaat tccgcggctt tgcccgtccc gccgcccagg
     3181 agccccgcat gtcgcaactc gcctatcgcc gcgtcctgtt gaagctctcc ggcgaagccc
     3241 tgatgggcga cgaggactac gggatcgatc ccaagatcct caaccgcctc gcccgcgagg
     3301 tgatcgaggt gcgcgacgcc ggcgtggaga tcgcgctggt ggtcggtggc ggcaacatct
     3361 tccgcggcgc cggcttggcc gccggcggga tggaccgggt caccggcgac cagatgggca
     3421 tgctggccac ggtcatcaac gcgctggcga tgcaggacgc gctggagaaa ctcggtgcca
     3481 agtgccgggt gatgagcgcg ctgaagatca acgacgtctg cgaggactac atccgtcgcc
     3541 gcgccatgcg tcatctcgag aagggccgga tcgcgatctt cgccgccggt accggcaatc
     3601 cgttcttcac caccgattcc ggcgcggcgc tgcgcgcgat cgagatcggc gccgacctgc
     3661 tgctgaaggc gaccaaggtc gatggcgtgt acgacaagga cccgaaccgg cacgccgatg
     3721 cggtgaagct cgacgacctg cgctacgacg atgtcatcgc ccgcaacctg caggtgatgg
     3781 acaccgccgc gttcgccctg tgccgcgatg ccgggctgcc gctgcgcatc ttcgacatgg
     3841 cccagcccgg cgtgctgctg cggatcctgc gcggcgagaa gatcggcacc ctggtccacg
     3901 cctgacgcgg gccgcggcga tcggcaggga ctcgcccgca tcggctcgct tataatcccg
     3961 cggttgccac acgaccacgg agcaccgcga tgctgaacga gatcaagaaa gacgcccagg
     4021 cccgcatggg caagagcatc gaatcgctgc gccatgccct ggtcaaggtc cgcaccggcc
     4081 gcgccaatgc cggcctggtc gacagcatca aggtcagcta ctacggctcc gagatgccgc
     4141 tggcgcaggt cgccagcgtg gcggtgaccg atgcgcgctc gatcaccatc accccatggg
     4201 aaaagcagat ggtcggcccg gtggaaaagg cgatcctggc ctcggacctg gggctgaccc
     4261 cgaacaccgc cggcaccgtg atccgcctca acatcccggc cctgaccgag gagcgtcgca
     4321 gggacctggc caaggtcgtg catggcgaag gcgaggacgc gaaggtcgcg atccgcaaca
     4381 tccgccgcga tgccaaccac caggtcaagg aactgctgaa ggacaagcag atcaccgagg
     4441 acgacgccag ccgcaccgag gccgagatcc agaagatcac cgacggcgcg atcaaggacg
     4501 tcgatgaggt gatcaagggc aaggaagcgg aactgatgtc cgtctgatcg gcctgctgcg
     4561 cttggccatg cggcgtcatc gcggtgcccg gcggcagtca cgtactgtct gtacgctcct
     4621 gccgccgggc atcgctcttc cttgcttggc ctgcgctcgc gacggccgat tggcatgagc
     4681 ggggcaaacg gcacggggcc catcccgcag cacctcgcca tcatcatgga tggcaatggc
     4741 cgctgggcgc agcagcgcca ccgcccgcgg atcatcggcc accgcgccgg cgcgcgtgcg
     4801 gtcaagcgca gcgtggagtt ctgcctggaa cgcggggtcg gcgcgctgac cctgttcgcc
     4861 ttttccagcg agaactggaa ccggccgacc gaggaagtcg gcggcctgat gaaactcttc
     4921 ctgggcgcgc tggaacgcga ggtcgatgaa ttgcatcgcc tcggtgcgcg cctgcgcttc
     4981 atcggcgagc gcagccgctt cgacgggccg atcctggcgc gcatgcaggc ggcggaagca
     5041 ctgaccgccg gcaacacccg cctgcaactg aacatcgccg ccagctacgg cggcaggcag
     5101 gacatcgccc aggccgcacg cgccctggcc gaggaggtcg ccgccggacg cctgcggccc
     5161 gagcagatcg acgaggacgc gctgtccgcg cgcatggcgc tggccgacct gcccgcgccc
     5221 gacctgttca tccgcaccgg tggcgaattg cggatcagca acttcctgct gtggcagctc
     5281 gcctacaccg agctctggtt caccgatacg ttgtggcccg acgtggatgc ggccacactg
     5341 cagcgcgcgc tggaccacta cgccggccgc gaacgccgct tcggcctgac cagtgcacag
     5401 gtcgcgggcc acgccaccgg gggaatcgag tgaccaagac gcgcgtgctg gccgcactga
     5461 tcatggcccc gctggcgatc ggcggcgtgc tgctcctgcc caccccttgg ctggtggcga
     5521 ttgccgcggt gctgttcctg ctcgcactgt gggaatggct acgactggcg gatgtcgaag
     5581 acaccctggc ccggaccatc ctgctggctt gcaatctcgc cctgatggtc gcgctggtct
     5641 ggggctcgcg ctcggcccac ggcgggtcgt tcgcactgtt ccagttggtg atcgtgatcg
     5701 gcgtcggctg gtggctgctt gccctgctgt ggctgaagca ctaccacttc gcctccgacc
     5761 acgactcgca cgcgcgtgcc ttcaagctgc tcgccggcac gctggcggtg gtgccggcct
     5821 ggtgcgcatt gggtgtgatc cacgccagcg aaccgaatgg ccattactgg ctgctgctgg
     5881 ccctgatgct ggtctgggcg gcagataccg gcgcctattt caccggccgc aacttcggcg
     5941 gcaagtggtt cggtggccgc aagctggcgc cgcggatcag cccgaacaag accatcgagg
     6001 gcctgctcgg cggcatcgcg ctggcgatgc tggtggcgat ggtcggcgcg ctgctgatcg
     6061 gcgtcgacca ggcgcacctg ccggccgtcg ccggcgtggc ggcggtgacc gtggcgttct
     6121 cggtggtcgg cgacctctac gagagcctgc tcaagcggca cgtggacatc aaggattccg
     6181 gccacctgat ccccggccac ggcggcgtgc tggaccgcgt ggacagcgtt ctcgccgcat
     6241 tgccggtgtt tgccctgggc aaggtctggt tcggtttctg agcgggtcga gcatgcaacg
     6301 cgttgccatc ctcggcgcca ccggttcgat cggcgcctcc gcgctggacg tgatcgcgcg
     6361 ccatccggat cgcatgcgcg ccagcctgct cgccgccggt acgcgcgtgg acgaattgct
     6421 ggcgttgtgc cgcgtccatc gtcccgagca tgcggtgatc gccgatgcgg gcgcgtatgc
     6481 cgccttgcgc gagggactgc gcgatgccgg cctggacacc caggcgcacg ccggcgccgg
     6541 cgcgctcgat gcgctggtcg ccggcgatgc ctgcgacagc gtggtcgcgg ccatcgtcgg
     6601 cgccgcgggc ctgccctcga cgctggctgc ggcgcgcgcc ggcaagcgcc tgctgctggc
     6661 caacaaggaa tcgctggtgc tggccggcga gctgctgatg caggcggcca gtgcgaacgg
     6721 cgcggtgatc gtgccgatcg acagcgaaca caacgcgatc ttccaatgcc tggccggcaa
     6781 tcccggcgag atgcccgcgc ggatcaccct caccgcctcc ggcgggccgt tccgcggctg
     6841 gacgcgcgag gcattggcca cggtcacgcc tgcgcaggcg gtggcgcatc ctaagtggtc
     6901 gatgggcccg aagatctcgg tggactcggc atcgctgatg aacaagggcc tggaagtcat
     6961 cgaggcgcac cacctgttcg gcatgtccgg cgagcgcatc cgcgtgctgg tgcatcccca
     7021 gtcgctggtg cattcgttcg tggacttcgc cgatggcagc accctggcgc aactgggctt
     7081 gccggacatg cgcaccgcct tggcagtcgg cctgggctgg ccacgtcgga tcgcctccgg
     7141 cgtgcccgcg ctggacctgc tggcggaagg cggcctgctg gagttcgccc cgcccgacct
     7201 ggacgccttc ccgtgcctcg ccctcgcatt ccgcgcattg caggcaggcg atgccgcgcc
     7261 ggcggtgttg aacgccgcca acgaagaggc tgtttcagcg tttcttcagg ggcggatcgg
     7321 tttcctgtcc atccccgacg tcgttgccca cgccctggac gccatgcccg cgacgccagc
     7381 cgattcgatt gatgcgttgc tggacgccga tgcctgggcg cgcgcgcagg cctgcgcctt
     7441 catcgcccgc aaggccgcgt gagtccatgc gcatgagcct gcgccgatga gcaccttcct
     7501 cgggtccctg tggtggatgc tggtcgcgct gggcgtactg gtgaccttcc acgaattcgg
     7561 ccatttctgg gtcgcccgcc gctgcggcgt caaggtgctg cgtttctcga tcggcttcgg
     7621 ccggccactg tggactcgcc acggcaagga cggcaccgaa tacgtggtcg ccgcgttgcc
     7681 gctgggcggc tacgtgcgca tgctcgacga gcgcgaggac cacgtcccgg cgcatcagct
     7741 ggaccaggcc ttcaaccgca agcccgtgct gcagcggatg gcgattgtcg ccgcgggccc
     7801 ggccgccaac ctgctgctcg cgttcgcact gctgtggacg atgctggtga tcgggcggcc
     7861 ggactacgcg ccggtgatcg gcaaggcgac cgggatcgcc gcgcaagcgg gattgcgcac
     7921 gggcgatcgt atcctcgccg tgggcgaacg cgagacgccg acctggagcg aggccgggat
     7981 ggcgctgact gtcgccgtcc tcgaccgcca acccgtgcaa gtgcgcgtac agggcggcga
     8041 tggcgaagag tcgacgcgga cgctggcgct ggatcgcctg cccgccagca tcgatgagct
     8101 gcgcgccctc gaggccaccg ggctcatcgc ccggcaccag ctctggcccg ccatcgtcgg
     8161 cagcgtccgc gcggataccc cggcctgggg cgtgctggcg gagggcgacc ggatcaccgc
     8221 gatcgacgcc accccgatcg ccagcttcga cgacatcccg cctgccgtgg ccgcattggg
     8281 cacccgcggc ggtcccggca tggtcgagat tgaacgggat ggcgagcgct tggcactcga
     8341 actgaccccg gtccggctgg tcgtcggacc cggatcatcc gcgtgggcac tcggggtggc
     8401 accggcggag cggcgaatgc cagcgacgga tgccgtgctg cgggtgggac cgcttgatgc
     8461 gatccccgcc gccgtgcgcg aaaccggctt ccagatgcgg caactggtcg agatggtcgg
     8521 gcgtgccttc gccggcaagg tctcggtgca gaacaccgtg gccggcccgc tgacgatcgc
     8581 ccaggcggcc aacgacttcg ccagccgcgg cgccgcctgg ttcctgaact tcctggccct
     8641 gttgtcggtc agccttgcct tgatcaacct gctgccgatc cccgtcttgg acgggggcca
     8701 cctgctgtat taccttatcg agttggccag aggccgcccc ttgggcgaac gcgccatggc
     8761 cgccggccag tacctcgggc tcgcgctggt agcgggcctg atgggcctgg cgttctacaa
     8821 tgacatcctg cacctggtgt catgacgccc cgcttccact tccctgcccg cgccgctggc
     8881 gccgtgcagc cccccgcaac cggatcctcc gaatgacccg acccacgtcc cgccgcctgc
     8941 ttgcccttgc cctcgccacg gcgctctcga tgccggcctg ggcacagacg gcgttcgcac
     9001 cgttctcggt cagcgacatc cgcatcgacg gcctgcagcg catcggcgcg ggcaccgtgt
     9061 tcacctacct gccggtcgag cgcggcgaca ccctcgacca ggccaaggcg gccgaggcat
     9121 tgcgtgcgct gtacaagacc ggcttcttcg aggacgtgcg cctggaccac cagggcagca
     9181 tcctggtgat caccgtggtc gagcgcccgg ccatcaacaa gctgaccctg gtcggcaaca
     9241 aggacctgaa gaccgaggac ctgctgaagg gcctgaagga catcggcctg gccgagggcg
     9301 agaccttcaa ccagctcaac ctggaccggg tcacccagga actcaaccgc cagtacaaca
     9361 accgcggcaa gtacagcgtc tcgatcagcc ccaacgtgga gaagctcgat cgcaaccgcg
     9421 tcaacctgac cttgaacatc aaggaaggca aggccgcgaa gatccgccac atcaacctga
     9481 tcggcaacga gtccttcccc gaggaggaca tcaccaaggg ctgggaatcg cacaccagca
     9541 actggctgtc ctggtacaag cgcgacgacc agtattcgcg cgagaagctg tccggcgaca
     9601 tcgaaaagct caatgcctgg tacctcgacc gtggctacgt cgatttcagc ctggattcga
     9661 cccaggtggc gatcagcggc gaccgcaagg acatgtacct gaccgccggc atgaccgagg
     9721 gcgaggtgta cagcgtgtcc acggtgaccg tctccggcga gaccgtgctg ccgaaggagg
     9781 agatcgagac catcgtcgcc ttcatccgcc ccggcagcac cttctcccgc ggcttgctgg
     9841 agctggcgac cgatgcgatc tccgcgcgcc tgtccaacat cggctacgcc ttcgccagga
     9901 tcaacccggt gccgcagatc gaccgcgaga agcgcaccgt cggcatcgac ttccaggtcc
     9961 agccaggccc ccgcgtcaac gtgcgccgga tcgtgttcaa gggcaatacc cgcacggccg
    10021 accaggtgct gcgccgcgag atgcgccagt tcgaaggcac ctggtattcg caggccgcga
    10081 tcgaccgcag caaggtccgc ctcgaccgcc ttggctactt cgaggaggtc aacgtcgaga
    10141 acgagccggt cgccggcacc gacgaccagg tcgacgtggt ctatacggtc aaggagacca
    10201 cctccggcag catcgccgcc ggcgtgggct actcgcagct gtccggcgtg aacctgtcgt
    10261 tgcagttgtc cgagaacaac ttcctcggca ccggcaaccg ggtgtcgatg tcggtcaccc
    10321 gcagcgctta ccagaagcgc tacgacttct ccttcatgaa cccgtacttc accgacgaag
    10381 gcctgtcgct gggctacaac ctgtggtggc gcgagttcga ctactccagc ttcaacgtcg
    10441 cgtcctattc caccaacagt ggtgccgcgc aggtgttcct gggccttccg ctgagcgaga
    10501 gcgattcggt ctcgctgatg ttcggcatcg actccaacga gatcctggcg ttcccggggt
    10561 ccacgccgcc gccgctggtc gactacatca gcgccgtggg caaccagacc ttccactcgt
    10621 ggcgcgccca ggtcggctgg ggccgcgata cccgcaacaa ctacttcacg ccggtggtcg
    10681 gcaccaccca gaacctgtcg gccgagatcg cgttgccggg ttcgaccgtg gagtacttca
    10741 aggtcgcata cgacttcgcc aagtactggc cgctcagcca ggcgctggtg ctgaagaccg
    10801 gcgtgaacct gggctacggc gactcctacg gcaaggactt cacccgcaac ctctgctaca
    10861 ccgcgccgac ctacaccgac accaacaacg acggcatccc ggataccgtg gtcccgggcc
    10921 cggcgccgac cgacccctgc ctcccgacgt cgccggactt cgacaagacg gtcaccgcca
    10981 gcggcctgcc gttcttcgag aacttctacg ccggcggcat ctcctccagc ggcaaggtgc
    11041 gcggcttcac cgacaacacg ctgggtccgt tctaccagac cgcatccggc ttccgccagc
    11101 cgctgggtgg atcgttcctg gtcgcgggtt cggtcgaggc gatcttcccg aagctgttcg
    11161 acagcccggc ggcacgcatc tcggcgttcg tggatttcgg caacgtgtac aacgggttcg
    11221 acaacttcga tgccggcgaa ctgcgcgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnngt ggtctcgcca ggcgcgcaca tcggcccgtt caccagcatc ggcgcgcgca
    11401 cgaccatcgg cgccggcgtg gtcgtcggtc ctggctgcgt gatcggcgac gactgcgaga
    11461 tcggcgaagg cagcgagttg caggcacggg tgacgctggt gacgcgcgtg cggctgggca
    11521 agcgcgtgcg gatcctgccc ggcgcggtgc tgggcgccgc cggcttcggc ctggcgatgg
    11581 aacacggccg ctggctcaac atcccgcaac tgggtggcgt ggtggtcggc gacgactgcg
    11641 aaatcggcgc caacagtacc atcgaccgcg gcgcgctggg cgacaccgtg ctcgaggaag
    11701 acgtgcgcct ggacaaccag atccaggtcg gccacaacgt gcgcatcggc gcgcacaccg
    11761 cgatggccgg ctgttccgcg gtcgccggca gcgcgcgtat tggccgctac tgcctggtcg
    11821 gcggcggtgc cggcgtcctc ggccacctgg aggtctgcga ccgcgtggtc atcaccgcca
    11881 tgtcgctggt gacccattcg ctccgcgagc ccggcgagta ctcctcgggc accccattga
    11941 tggacaatcg cagctggcgc aagagcgcgg cgcgcttcaa gcaactcgac cgcatggccc
    12001 gcgcgaaccc gcgcgccggc gcaggcgaca aggaccagga atgaccgaga ctcccttccg
    12061 cgtgaccatg ccggtgaaca ccccggagat cgaacgcctg cttccgcacc gctatccgtt
    12121 cctgctggtc gaccgggtga tcgagttcga gaaggacaag cgcgtgctgg cctacaagaa
    12181 cgtgacctgc aacgagccgt tcttcaccgg gcacttcccg ggccgtccgg tgatgccggg
    12241 ggtgctggtg gtggaggcgc tggcccaggc cggcggcctg ctcacccagc tctcgcgcga
    12301 tccggcggag atcggcaagg acaccttcta cctggtcaag gtggacaacg cgcgcttcag
    12361 ccgcatggtg gtgccgggcg accgcctgga actcgaagtc gaattgaagc ggcgcatccg
    12421 caacatggcg cagtacgtcg gcatcgcccg cgtcgatggc gagcaggccg cctgtgccga
    12481 gatcctctgc gctgccgccc gcgaatgagc aacgcgaccg tcctggtcca tcccaccgcc
    12541 gccatcgatc cggatgcgcg gctgggtgcg ggcgtgcacg tcggcgcgta tgcggtgatc
    12601 ggtgccggcg tggagatcgg cgatggctgc cgcatcgggc cgcactgcac gatcgaggga
    12661 ccgacccgga tcggacgcga caacgtcttc cacgcgcatg ccgcgatcgg cggcgaaccg
    12721 caggacaaga agtaccgagg cgaacgggtc gaactggtga tcggcgacgg caacgtgatc
    12781 cgcgaattcg tcaccatcaa ccggggcacc ggcgatggtg gcggcatcac ccgcatcggc
    12841 gaccgcaact ggatcctggc gtattgccac gtggcccacg actgcatcat cggcaacgac
    12901 tgcgtgttct ccaacaacgc cacgctggcc ggacacgtga cggtgggcaa ccacgtgatc
    12961 ctcagcgggt tcgccggcgt gcaccagttc tgccggatcg gcgaccacgc gttcatcggc
    13021 atgggtgcgt tcgtcaacgg cgacgtgcca ccgttcctga tggtggcgca ggaaaaatac
    13081 gcgcggccac gcggcatcaa tgcggaaggc ctgaagcgac gcggtttcga tgcaggccgc
    13141 atcgccagca tcaagcgcgc ctatcgcgcg ctgtacatgg gcgacgcgaa gctggacgaa
    13201 gccaagtccg agctcggcga gatcgcggct agcggcagcg acgatgtgcg cgcgatgctg
    13261 gacttcatcg aacgcggcga gcggccgctg ctgcggtgac ccactcaggt agcgtcgtgc
    13321 cgggtgccgg ggtgttttcc aacaaagcga gtatcgggga gcgacttggg aaataccccg
    13381 gcacccggca cgacgcggat cgaatccgag gaaaagcatg agcctccgta tcgccctctg
    13441 cgccggcgag acctccggcg accagctcgg cgccggattg atcgaggcgc tacgcgcgca
    13501 tttccccgat gcggaattcg ccggcatcgg cggcgatgcg atgcgcgcgg ccggcatgga
    13561 cacctggttc gatgcgagcg aactggcggt gatgggcctg gccgaagtgc tggcccacct
    13621 gccccgtctc ctgaagttgc gcaggacgtt tcgccagcgc gtgctggact ggcagcccga
    13681 cgtcttcatc ggcatcgacg cgcccgactt caacctcggc gtcgaacgct ggctcaagca
    13741 acgcggcgta cgcaccgtgc acgacgtgag cccgtcggtg tgggcctggc gcgaatcgcg
    13801 cgcggcgaag atcgccgcca gcgcagatcg cgtgctctgc ctgttcccga tggagccgcc
    13861 gatctacgcg cgccacgggg tcgatgccgt gttcatcggc catccgctgg ccgacgcgat
    13921 cccgctgcag ccggatcgcg ctgccgcgcg ccacgcgctc ggcgaatcgc gggacgggcc
    13981 gatcctcgcg ttgctgcctg gcagccggct cggcgaaatc caacggatgc tgccgacgtt
    14041 cctggaggcg gcacgcctcc tgcatccgga tgtgcccggc ctgcgcctgc tgatccccgc
    14101 ggcgaatgcg caatgtcgtg ccgcgatcga cgcgatcgtt ggcgatgcgc cggcgatgcg
    14161 cgtgctcgat ggcaatgcac aacgcgcgat gatcgccagc gatgtcgtct tgctggcatc
    14221 ggggaccgct gcactggaag cgatgctgtg caagcggccg atggtgatcg gtcatcgcat
    14281 ctccggcctg acctacgcca tcgtcaaggc gttcggcctg ctgaagtcgg cgcatgtcag
    14341 cctgcccaac gtgctggccg gcgaaacgct ggtgcccgag ttgctgcagg acgactgcac
    14401 gcccgcgaac ttgcaggccg cgctgctgcg ctggttctgc gatgccgatg cggtggaggc
    14461 gttgcaaccg aggttcctcg cgatccacga aacccttcgc cgcgacgcct ccgcgcgcgc
    14521 cgccgacgcg gtcgccggat tggtggggac gcccgcatga tccagcgcat cgccgggatc
    14581 gacgaagccg ggcgcggccc cttggccggg ccggtggtgg tggccgcggt ggtgttcccg
    14641 ccgggccgca cgccggtgaa cggactggac gattccaagg cgttgaccgc gcgcaagcgc
    14701 gaggcgctct acccccgcat catcgagcgc gcgctcgcgt ggaagatcgt gttcatcgcg
    14761 cctgccgaga tcgaccgacg caacatctat cacgccaccc tgcacggcat gcgcgaggcg
    14821 ctgctgggcg tggcacacat ggccgacgtg gcccgcatcg acggcaaccg cctgccgccc
    14881 gacctgccct gcccggccga ggcctggatc ggcggcgatg cacgcgaccg cacgatcatg
    14941 gcagcctcta tcctggccaa ggtcgcccgc gaccaagcca tgcgcgagct gcacctgctg
    15001 tatccgcact acggcttcga ccagcacaag ggctatccca gcccgtcgca cctgcaggca
    15061 ctgcgggagc atggcccgtg cccgcaccat cggcgcagtt tcgccccggt gcgcgactgc
    15121 ggcggcaccc cgatgcaagg ggatttgctg gcccagaccg caccgtgctg agctcgcgcg
    15181 ggcctgcctg cgtgtcaagc cttgccgggg tcccggggcc ggcctacgct cgcctttgag
    15241 cctgcggatc cgcgatgcca gcccgtttcg ttcccctgca cctgcacagc gagtactcgc
    15301 tggtcgattc gacgatccgc atcccggagc tcgtcgcggc ctgcgctgcg cgcggcctgc
    15361 cggcggtggc gatcaccgac cagaacaacc tgttcgcgct ggtcaagttc tacaaggagg
    15421 cggaaaaggc cgggatcaag ccgatcgccg gcgccgacgt gctggtcgcc gagggcaccg
    15481 ccgcgccctc gcgcctgacc ctgctgtgcc gcgacaacga cggctacctc agcctctcgc
    15541 aaatcctcac gcgcgcgtgg atggagggcc atcgcgtcga tggcgtggtc gcacgcccgg
    15601 cgtggcttgc ggagaacgcc ggcctgttcg caatcgccgg gccgcagtcg gagcccggcc
    15661 agctgttcgc atccggtcgc cacgacctgg ccgaacaggc actggccgac ctgcaggcga
    15721 aattcggcga tcgcctgcac ctggaattgc tgcgcaccgg tcgcgccggc gaggacgcct
    15781 tcaacgcgtt cgcgctggat gtgtcggcgc gccgaagcat cccgctgctg gccagcaacg
    15841 acgtgcgatt cctcgacgcc gacggcttcg atgcgcacga ggcgcgcgtg tgcatcgcca
    15901 gcggccgggt gctggacgac acccgccggc cgcatgacta cagcccacag caatacctca
    15961 agtccgccga cgagatggcg gcgctgttca tcgccgatgc accggacgcg ctggacaaca
    16021 ccgtggcgct ggcgcagcgc tgcaacctgg gactgcggct gggcacctat tacctgccgg
    16081 cgttcccggt gcccagcgac gaaaccctgg acagctggat ccgcagccag tcgcgcgagg
    16141 ggctggtcaa acgcctcgag aaagcgccac tggcgaccgg caagacccgc gcggactacg
    16201 aggcccggct cgagctggag ctggacgtca tcgtcaagat gggcttcccc ggctacttcc
    16261 tgatcgtcgc cgacttcatc aactgggcga agcagcatgg catcccggtc ggccccggcc
    16321 gcggttccgg tgccggctcg ctggtggcgt gggcgctcgg catcaccgac ctcgacccgc
    16381 tgccctacga cctgctgttc gagcgcttcc tcaatcccga acgcgtgtcg atgccggact
    16441 tcgacatcga cttctgcatg gaccggcgcg acgaagtgat cgactacgtc gcgcggaagt
    16501 acggccgcga ccgcgtcagc cagatcatca cctacggcac gatggcggcg aaggcggtgc
    16561 tgcgcgatgc cggccgcgtg ctcggtttcc cgtacggctt cgtcgatggc ctggccaagt
    16621 tgattccgct gcagcccacc gacccgatga cgctggaaga cgcgatcggc atcggcaagc
    16681 gcgcgcagaa ggaaccggac cgcgtcgtca ccgagttccg cgaccgctac cacgccgagg
    16741 acgacgtccg cgacctggtc gacctggccc tgcaactgga agacctgacc cgcaacgccg
    16801 gcaagcacgc cggcggcgtg gtgatcgcgc ccagcccgct gcaggatttc tgcccgctgt
    16861 tcgcggaaca cgacggccac ggcctgggcc gcaacccggt gacccagttc gacaaggacg
    16921 acgtcgaatc gatcggcctg gtgaagttcg acttcctcgg cctgcgcacg ctgacgatca
    16981 tcgactgggc ggtgaaggca atcaacgtgc gccgcgccgc cgcgggcgaa agcgcattcg
    17041 acatcatgtc gctgccgctg gacgacaagg ccacgtacga actgttcgcg cgcggcgatt
    17101 cggtagcggt gttccagttc gaatcgcgcg gcatgcgcga actgctcaag cgcgccaagc
    17161 ccgacacctt cgaggacatc atcgcgctgg ccgcactgtt ccgccccggc ccgctgggca
    17221 gcgggatgga caagcaatgg tgcgaccgca agcacgggat cgaggatgtc agctatccgc
    17281 accccgcgct ggagcaggtg ctggcgccga cctacggcgt gatcgtgtac caggaacagg
    17341 tgatgcagat cgcccaggtg ctggccggct attcgctggg cg
//
LOCUS       DAOOCX010000045        16104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3260_length_16104_cov_9.636364, whole genome shotgun sequence.
ACCESSION   DAOOCX010000045 DAOOCX010000000
VERSION     DAOOCX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 16104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16104
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3260_length_16104_cov_9.636364"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PK399_06055"
     CDS             <1..392
                     /locus_tag="PK399_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPW12715.1"
                     /translation="LEGALKLKEISYIHAEAYPAGELKHGPLALVDDDMPVVVIAPND
                     ALLEKVKSNIQEVRARGGEMFVFADADSHFGESEQVHVIRTPRHVGVLSPIVHTIPVQ
                     LLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            complement(453..677)
                     /locus_tag="PK399_06060"
     CDS             complement(453..677)
                     /locus_tag="PK399_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12716.1"
                     /translation="MTMVICRECKAQISTLAAACLHCGATKRKTANTTSVAIAFLGIT
                     LLANTWMLWRLYDIESRMAQGVVAQGHGSP"
     gene            883..1956
                     /locus_tag="PK399_06065"
     CDS             883..1956
                     /locus_tag="PK399_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW12717.1"
                     /translation="MLRALCLSLFLFACTGSAAARAAPAGDAGVQAAMALPDDLRRML
                     PADVVNRRIDEAIRAQALIDFMIADEGLALRYQEQPTLGIAGSFAQRRVNCLSFTMMF
                     VAMARASGLKAFAQASDNALSMRMLDGTLFRATHVNAGVLIDGEHHSVDVGWRGVAAG
                     RKPRRIPDARMVALLHNNHAVEHLLQGEAAAAAEAIAAALALDAGSATLWSNAGVIHA
                     RAGRRDEAERAYLRAISLQRDHIGALANLVGFHRAGGDAAQLAFYEKRLQRAQALDPF
                     SQFLIGQARAEAGVPAEAIVHYRRAIRMLPDEPEFHRSLADAYQALGDPVAATRARDR
                     ASRLEAKQEAERGIRDADLPGPG"
     gene            complement(2048..3931)
                     /gene="btuB"
                     /locus_tag="PK399_06070"
     CDS             complement(2048..3931)
                     /gene="btuB"
                     /locus_tag="PK399_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor"
                     /protein_id="HPW12718.1"
                     /translation="MQSCNTQLRSSALALALSLPSLAFAQSDEAKDLDNLVVTATRTA
                     TTADATLAAVEVIDRRQLDASSARSLPELLRGRAGITIVNQGGMGKLSTLFLRGTESD
                     HTLFLVDGIRVGNSTSGLTSLQDIPLVQIERIEIVRGPRSSLYGADAIGGVIQVFTRR
                     GAGGEGVHGRGRIAAGSHGLREASAGIDLRGARGGVGVDLAHQETDGINACTGFYNPL
                     TFAGAGCFIVPGTHLDRDGYRNDSATMRADFAPGEAWQLDARASRAEGHNDYDGDYQD
                     NSDIVQQVVGGSVRWSPSDAVAVKLTAGRNTDDSDNFSGSTFANRYNTTRDSATLQGD
                     FTVSPDHVVTAGFDWLRDRANVVDPWSPFAASRGNRAAFAQYQGSFGAHDLQLSARRD
                     DNDQFGGHGTGGIAWGMGFGEAWRVTASHATAFKAPSFNELYYPGYSNPLLKPEASRS
                     TELSLRHERASWSVQANAYQTRVDDLIAYDSRLFAPNNVEQARIRGIELVATAQLAGW
                     ELRGQVGTLDARNLSSGPNQGKRLARRPQRSARLDLDREVGAWGFGISGIAEAARWDD
                     VANTLRVGGYGTVDARVSWRFAADWTLQANLVNAFDRDYATSAWYRQPGREFALSLRW
                     QPN"
     regulatory      complement(4052..4303)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4449..4832)
                     /locus_tag="PK399_06075"
     CDS             complement(4449..4832)
                     /locus_tag="PK399_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HPW12719.1"
                     /translation="MSTTAPKLRNIGPKSAAWLRQVGLRSLQDVAAIGAVDAFMKVRR
                     AGFKPSLNLLYSLEGALQDCHWQEVPEARRTELVQAAEAAIALLPPPRGRPAATPVTT
                     TQYEDTSVPALALFDDDRGGPNVSE"
     gene            complement(4829..5317)
                     /locus_tag="PK399_06080"
     CDS             complement(4829..5317)
                     /locus_tag="PK399_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPW12720.1"
                     /translation="MSFSTIPLTGSKPDQYAQLLEQARALLHGERDRIANAANLSALV
                     YHALPGLNWVGFYFYDGHELVVGPFQGLPACVRIPLDKGVCGAAARTGETQRIEDVHA
                     FPGHIACDSASNSELVVPLVGANGELIGVFDLDSPLHARFSVEDQQGLEAIARAFLES
                     LQ"
     gene            5383..6120
                     /locus_tag="PK399_06085"
     CDS             5383..6120
                     /locus_tag="PK399_06085"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HPW12721.1"
                     /translation="MDLSTLPIDGGVRQRGEQVTRLETFVDAAFAFAMTLLVVSFDAM
                     PESFAQLYDALRRLPAFLAGFAILAMFWQAHHRFSRRFGLEDTTIVLASMLLVAATLF
                     YVYPLRMVMSAAMGFFTGGWAPSEFKVQSFLEFRLIFVIYGVGFAILAAIIAWMNVHA
                     LRKAAVLQLDAAERLAAESEVALNLVLIACAVLSVAVALVIPPSVGWMQALPGSVYAL
                     LGIAMPVLSIRYGRRIQALAAARDAAP"
     gene            6117..6830
                     /locus_tag="PK399_06090"
     CDS             6117..6830
                     /locus_tag="PK399_06090"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HPW12722.1"
                     /translation="MIAALERGDKATRVEAFVDAAFAFALTLLVIAGDRIPGSVEELL
                     QALKSLPAYALGFLLMLKFWTAHVAWSRRYGLDDATTRRLSLLLVFLVLVFVYPMKMV
                     FGALCSALTFGWLPANFVITSLREVPVLFIVFGIAFGSLGMVMWLLYAHAWRRRDEIG
                     LSARERIDTQVKLWTWALVPVVALLSIASALLIPARPGIGWWLGFPGFLYFSLNIATP
                     LLDAWSRRLQARMPIEGGA"
     gene            6830..7507
                     /gene="bioD"
                     /locus_tag="PK399_06095"
     CDS             6830..7507
                     /gene="bioD"
                     /locus_tag="PK399_06095"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HPW12723.1"
                     /translation="MFSVYISGTDTGIGKTLASSALLHALRGHGLRAIGMKPVASGCD
                     CIDGEWKNADALALQAAGQPGIAYADINPFALEHPLAPELAARDAGIEVSLPPILAAH
                     ARLAAQADALIVEGVGGWAAPLSASLMQADLVRALRVPVLLVVGLRLGCINHALLSAR
                     AIAADGVHLAGWIASHVDPEMERVDDNIAMLRARMPAPCWGVLPHVPAADPARLASLL
                     RIPMLAE"
     gene            complement(7702..10884)
                     /locus_tag="PK399_06100"
     CDS             complement(7702..10884)
                     /locus_tag="PK399_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW12724.1"
                     /translation="MKFTDLFIRKPVLALVVSLFILLFGLRAFSELNVRQYPEMRNAV
                     VNISTTYFGADADLIQGFITTPLEREIASAEGIDYLTSTSAAGISTIQAYIQLDKDPS
                     EALTQISAKVNKMRGQLPAESEAPVIDLQQGQQIAAMYVSLASETLDNNQITDYITRV
                     IQPKLVTIDGVQRADILGAGTFAMRIWLKPDRMTAYKVTASDVSNALSSNNVLAAVGS
                     TKGQMVAIDMTARTDLRNAEEFRKLVVREQDGAIVRLGDIADVVLGSESYGTSVRING
                     EAATFMGIFVAPDANSLDVIKDVRKVWDGEIVPQLPQGIKATIPYDSTEAIQDAINEV
                     ISTIIEALIIVIVVIFLFLGSMRSVLIPAVTIPLSLIGALFLMLLMGFTINLLTLLAM
                     VLAIGIVVDDAIIVLENIHRHIEEGMTPFDASIRGARELAWPVVAMTTTLIAVYLPIG
                     FQGGLTGVLFTEFAFTLAGAVLLSGVIALTLTPMMSSRILIAHDPDHPKGKLELWLDS
                     KFETLRHGYQRKLHGALDTKAVIAVFGAIVLVSCGFLYTSAPKEQAPLEDEGFIFSVA
                     SADPTATLDYVERYTDEIARMVKGVPEVQDYFLFNGGFGGSGGGASPMAMAGFVLKPW
                     SERTRSTNQVLQQDLQPKMSQVTGLNVFALVPPSLPSAGGGAGGGEFIIGGVGDLSQL
                     ADVADQILAKAMASKRFIFLDKDLKIDKPQIQVNVDRDKAATLGIDMRTLAADMAAML
                     SGGYSNRFAMQNRSYLVIPQVQRSDRLNASALDNYYTRTRSGELIPLSTIVSLSETTQ
                     PQSLKRFQQLNAVALTFAPRPGVSKGEALAILDQAAKDVLPQGYSVDYAGESRQFKQE
                     GQAMLLTLALALIVIFLVLSAQFESFRDALIMLLTVPMAICGALLALNVLALVANVLS
                     FSGIEAFPGMSINIYTQVGLVTLVGVISKHGILIVEFANKLQEQGMSKREAIEEATGI
                     RLRPVLMTTAALVFAMVPLLIAKGPGAASRFSMGVVIVSGMTIGTLFTLFVLPAFYLY
                     LARDHAHDADHQKAAEGEAASLPPPEPQHG"
     gene            complement(10987..12177)
                     /locus_tag="PK399_06105"
     CDS             complement(10987..12177)
                     /locus_tag="PK399_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW12725.1"
                     /translation="MSKAAAKRKPNTTLRWVLMLVGVAVVFGGVFAIKAFFAKQTNNF
                     FDNMPQPAVAVSSYVAKTQRWTDNGEAVGTFVAINGTDITTEAGGVIRAIEFEPGQRV
                     SAGTVLFRLNTANEEATLNALETSAKLANVQAERWQKLGKDKLVSLDDVQQRVAAAAS
                     ARAQVEAQRALIAQKTIRAPFTGVLGLRKVNLGQYVAPGAAMVSLQQLDPIYLDFSLP
                     EQQIGKMIEGTAVRASVDALPGQAFEGRVTAVEPLVDASTRNFKAQATFANPDGALRP
                     GSFAKVTFALGGVRDVLVIPQTAVSFNPYGNAVFVISKTARKPGEKDMQGKELTGDKF
                     IVTQRFIKTGATQGDLIVVTEGLKAGEQIVTSGLLKLRNDAEVTINDAIQPSADASPT
                     PENR"
     gene            12440..14629
                     /locus_tag="PK399_06110"
     CDS             12440..14629
                     /locus_tag="PK399_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPW12726.1"
                     /translation="MAVISNVATAAPPAQPDPDCVGVVLGGGGARGIAHIGVLKVLER
                     ERIPVCAVTGTSMGAIVGGLYATGYDAEELEKIVGAIDWADMFVDDPPRQELPMERKD
                     EDFRHLLDLEIGYKDGRLGFPVGLVRGQKLMLLLRRLTLSTWRSEDFDALPIPFRAVS
                     ADIVTGRKVVFDDGDLALAIRASMSVPGAFAPVKVGDQLLVDGGMAENVPIDEMRAMG
                     VSRMIVVDVGSKLLGEKDLTNPAVILDQMITALMTEKTERSLATLGAGDVLVRPELGD
                     ITSGQFNRTAEAVAVGERAAEAMLPQLRRHAVSAERYAALRARQHRRDFDPGLVAFLD
                     VEPGHTPSATRHVAWATEGLAGKPFDVDKVEHAVGRAYGDGRFEQIGYRLVERDGQAG
                     LEVIPTQKPWTAFGRIGLQLDDNFNGRNNYLLSAELTFNDANRSGGKWRNLLQLGRVT
                     GLRSEFVQPFGRAGAFYLKPSLEIRADSLPLSVDGEQVAEYRLVRRGLDIEAGYSPSP
                     EWRIGIAAIGGRDRARRIVGDSSTFAGGSETFAGVRVNATWDSLDSVSFPTKGLRATA
                     ELVSLHPWGTTILDGEVARASLDWAYSRDRYHLLLGMRLASALQDDGFFQAQTFLGGF
                     LNLSGFGERSLVGNQAALARAVVYRRVGDTTRIFAMPMFVGGSLEAGNAWDRRDDVDF
                     GSLLVGGSLFTGFDTPLGPIFLGYGRNQAGADSWYLTFGSLLRQEPK"
     gene            complement(14745..15134)
                     /locus_tag="PK399_06115"
     CDS             complement(14745..15134)
                     /locus_tag="PK399_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HPW12727.1"
                     /translation="MYQFNDQFTKATSQFADVAANANRLAIDNAQKAFGLQMAAFEEN
                     TSAAFAFWNELIEVRDVEGFKSVMPKGVQIARTATERSLGTAQEVFANTVKTNEAIGA
                     LAKGQFEKATAQATAEVEKVTKAASKK"
     gene            complement(15355..15846)
                     /locus_tag="PK399_06120"
                     /pseudo
     CDS             complement(15355..15846)
                     /locus_tag="PK399_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017167453.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            15978..16054
                     /locus_tag="PK399_06125"
     tRNA            15978..16054
                     /locus_tag="PK399_06125"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16012..16014,aa:Met,seq:cat)
BASE COUNT         2443 a         5311 c         5529 g         2821 t
ORIGIN      
        1 cgctggaagg cgcgctcaag ctcaaggaaa tctcctacat ccacgccgag gcctatccgg
       61 ccggcgagct caagcacggt ccgctggcgc tggtcgatga cgacatgccg gtggtggtga
      121 tcgcgccgaa cgatgcgctg ctggagaagg tgaaatccaa catccaggaa gtgcgcgcgc
      181 gcggcggcga aatgttcgtg ttcgccgacg ccgacagcca cttcggcgag tccgagcagg
      241 tgcacgtgat ccgcacgccg cggcatgtcg gcgtgctgtc gccgatcgtg cacaccatcc
      301 cggtgcagtt gctggcgtac cacaccgcat tggcccgcgg caccgacgtc gacaaaccgc
      361 gcaacctggc caagtcggtc acggtcgagt aaccgccgcg cgggccacgt gcctgcgtgg
      421 cccgcggtat cagggagccg cggcgtcccg tttcacgggc tgccgtgccc ctgcgcaacc
      481 acgccttgcg ccatccgcga ctcgatgtcg taaagccgcc acagcatcca cgtgttcgcg
      541 agcagggtga tgccaaggaa ggcgatcgcc acggacgtgg tgttcgccgt cttgcgcttc
      601 gttgccccgc aatgcaggca ggccgcggcc agcgtgctga tttgcgcctt gcattccctg
      661 cagatcacca tcgtcattgc gtcatccccg gttgcgtcga tccatgccgc cttgcggcag
      721 cgccctgtgc atcgccatcg ccccatgcac ggaccgccct gcggtccgtg gcctaaacct
      781 cgcacaggtc attagggcca ttcctctaat cggtgcgccc tcgggggcgc atactggccc
      841 cgctcccggc agttgcgccg gggcatgccg ggaccgagga cgatgctgcg ggcgctctgc
      901 ttgtcgctgt tcctgttcgc ctgcacgggc agtgcagcgg cgcgcgcggc gcctgccggc
      961 gacgcagggg tccaagcggc aatggcgttg ccggacgacc tgcgccgcat gctgccggcc
     1021 gacgtggtca accgccggat cgacgaggcg atccgcgcgc aggcactgat cgacttcatg
     1081 atcgccgacg agggcctggc cttgcgctac caggaacagc cgacgctcgg catcgccggg
     1141 agcttcgccc agcgccgggt caactgcctg tcgttcacga tgatgttcgt cgcgatggcg
     1201 cgtgcgtccg gcctcaaggc attcgcgcag gcatccgaca atgcgttgtc gatgcgcatg
     1261 ctggacggca cgttgttccg cgccacccat gtcaacgccg gcgtgctgat cgatggtgag
     1321 caccattcgg tcgatgtcgg ctggcgcggc gtggccgcag ggcgcaagcc gcgacgcatc
     1381 ccggatgcgc ggatggtcgc cctgctgcac aacaaccacg cggtcgagca cctgttgcag
     1441 ggcgaggccg ctgccgccgc cgaggcgatc gccgcggcgt tggcgctgga tgccggcagc
     1501 gcgacgctgt ggagcaatgc gggggtgatc catgcgcgcg ccggtcgtcg cgacgaggcg
     1561 gaacgcgctt atcttcgcgc catcagcctg cagcgcgacc atatcggggc gctggccaac
     1621 ctggtcgggt tccatcgcgc cggcggcgat gcggcgcagc tcgccttcta cgaaaagcgc
     1681 ttgcagcgcg cgcaggcgct cgatccgttc tcgcagttcc tgatcgggca agcgcgcgcc
     1741 gaagccggtg tcccggccga agcgatcgtc cactaccgtc gcgcgatccg catgttgccc
     1801 gacgaaccag agttccatcg cagcctggcg gatgcctacc aggcgttggg cgatccggtg
     1861 gcggcgacgc gcgcacgcga tcgcgcgtcg cgcctggagg cgaagcagga ggcggagcgg
     1921 ggcatccgcg atgccgatct tcctggcccg ggttgaacga cgcagtccac gccgcacaca
     1981 caagaagggc ccggcatcct tgccgagccc catgcgtcct tgcgcaaccg ctccttgatg
     2041 cgaagcctca gttaggctgc cagcggaggc tcagcgcgaa ttcgcggccg ggctggcgat
     2101 accacgcact ggtcgcgtag tcgcgatcga aggcattgac caggttggcc tgcagcgtcc
     2161 agtcggcggc gaagcgccag gacacgcgcg catccacagt gccgtaacca ccgacgcgca
     2221 aggtattggc gacatcgtcc cagcgcgccg cctcggcgat gccgctgatg ccgaagcccc
     2281 acgcgccgac ctcgcgatcc aggtccagcc gagcgctgcg ttgcggacgc cgcgccaggc
     2341 gcttgccctg gttgggaccg ctgctgaggt tgcgcgcgtc gagcgtgccg acctgcccgc
     2401 gcagttccca gccggccagt tgcgcggtcg ccaccagttc gatcccgcga atgcgcgcct
     2461 gctcaacgtt gttgggtgcg aacaggcggc tgtcgtaggc gatcaggtca tcgacccggg
     2521 tctggtaagc attggcctgc accgaccagc tggcgcgctc gtgacgcagc gacagctcgg
     2581 tgctgcgcga cgcctcgggc ttcagtagcg ggttgctgta gccggggtag tacaactcgt
     2641 tgaagctcgg cgccttgaac gcggtggcat ggctggcagt cacccgccag gcttcgccga
     2701 agcccatgcc ccaggcgatg ccgccggtgc catggccgcc gaactggtcg ttgtcgtcgc
     2761 gccgcgcgct gagttggagg tcgtgcgcgc cgaacgaacc ctggtactgc gcgaacgccg
     2821 cgcgattgcc gcgcgatgcc gcgaacggcg accacggatc gaccacgttg gcgcgatcgc
     2881 gcaaccagtc gaaaccggcg gtcacgacgt ggtcgggcga aacggtgaag tcgccctgca
     2941 gggtcgcgct gtcgcgggtg gtgttgtagc ggttggcgaa cgtgctgccg ctgaaattgt
     3001 cggagtcgtc ggtgttgcgg cccgcggtca acttcactgc aacggcgtcg gatggcgacc
     3061 agcgcacgct gccgcccacg acctgctgga cgatgtcgga attgtcctgg taatcgccgt
     3121 cgtagtcgtt gtggccctcg gcgcgggacg cgcgcgcgtc gagttgccag gcctcgcccg
     3181 gtgcgaagtc ggcgcgcatg gtggcgctgt cgttgcggta gccatcgcgg tccaggtgcg
     3241 tgccgggaac gatgaagcaa cccgcgcctg cgaaggtcag cgggttgtag aagccggtgc
     3301 aggcattgat gccgtcggtt tcctggtgcg ccaggtccac gcccacgcca ccgcgcgctc
     3361 cgcgcaggtc gatgccggcc gaggcctcgc gcaggccgtg gctgccggcc gcgatgcggc
     3421 cgcgcccgtg cacgccttcg ccgcccgcac cgcggcgggt gaacacctgg atcaccccgc
     3481 cgatcgcgtc cgcgccgtac agcgaggaac gcggaccgcg cacgatctcg atgcgctcga
     3541 tctgcaccag cgggatgtcc tgcagcgagg tcaggccgga ggtcgaattg cccacgcgga
     3601 tgccgtcgac caggaacagg gtgtggtcgg actcggtgcc acgcaggaac agcgtgctga
     3661 gcttgcccat gccaccctgg ttgacgatgg tgatgccggc gcgtccgcgc agcagttccg
     3721 gcagggagcg tgcgctgctg gcatccagtt gcctgcggtc gatcacttcg accgccgcca
     3781 gcgtggcatc ggcggtggtc gccgtgcgag tggcggtgac caccaggttg tcgaggtcct
     3841 tcgcctcgtc gctctgcgcg aatgcgagcg atggcaagga caaggcgagc gcgagtgcgc
     3901 tcgaacggag ctgggtattg caggactgca tgacgattct ccggcgcgca cgcccgcgcg
     3961 cccaacaggg aggggcggtg gcggcacgac ggacacggga gacgggcgaa tggacaacgc
     4021 atgcgcgacg tcgcgcaatg cccgccgcat cgcaaccaac agacttcagg gccggtctcc
     4081 gggctggcga gcggaacgac acgcccatcg agggcctgca ttcccgcctg gcgccttccc
     4141 atgccgcatc gtatcgatgc gggcacagtg gccgatgcca gggttatctc gcttaccgtt
     4201 gcgggggcag cgccggactg gatgcgcagg gcgcgtcgtc accggcttcc cgtttcaatc
     4261 ctcgggcggt tgccgtcgga tcaccttgaa gcgcgcgcag tgtacgcagc ggggcgggac
     4321 gcggcaaagg atatttagcc ttgaggcatg accgttcgtg gtgaacctgt cgaaccatgg
     4381 acgggcagcg cagcctgccg atggatcgac aggctcgcca ggaacggcgc cacggcttcc
     4441 tgcgacggtt actccgagac gttcggcccg ccgcggtcgt catcgaacag cgcaagcgcg
     4501 ggcacgctgg tgtcttcgta ctgggtcgtg gtgaccggcg tggcggcggg gcgtccgcgc
     4561 ggtggcggca gcagcgcgat ggcggcttcg gcggcctgca ccagttccgt acggcgcgct
     4621 tccggaacct cctgccagtg gcagtcctgc agggcgcctt ccagcgagta cagcaggttg
     4681 aggctgggct tgaacccggc ccggcgcacc ttcatgaagg cgtcgaccgc gccgatcgcg
     4741 gccacgtcct gcagcgaacg caggccgacc tggcgcagcc aggccgcgga tttcgggccg
     4801 atgttgcgca gcttgggggc ggttgtgctc actgcagcga ctccaggaat gcccgggcga
     4861 ttgcctccag gccttgctgg tcttcaacgg aaaaacgcgc gtgcagcgga ctgtccaggt
     4921 cgaacacgcc gatcaattcg ccattcgcgc cgaccagcgg caccaccagt tcggaattgc
     4981 ttgccgagtc gcaggcgata tggccgggga acgcatgcac gtcctcgatc cgttgggtct
     5041 cgccggtgcg cgcggccgcg ccgcacacgc ccttgtccag cgggatgcgc acgcaggccg
     5101 gcaggccctg gaatggaccg acgaccagtt catggccatc gtagaaatag aagccgaccc
     5161 agttcagccc cggcagcgcg tggtagacca gcgcggacag gttggcggcg ttggcgatgc
     5221 ggtcgcgttc gccatgcagc agggcgcgcg cctgttcgag caattgcgcg tactggtccg
     5281 gcttgctgcc ggtgagcggg atggtcgaga aactcatggc cgcagtgtag caaccgggtc
     5341 gatgcggatt accatcgccg cggaacccca ggggacgatg cgatggacct gtccacactg
     5401 ccgatcgatg gaggcgtgcg ccagcgcggc gaacaggtca cccggctgga aaccttcgtc
     5461 gatgccgcgt tcgcattcgc gatgaccctg ctggtggtct cgttcgatgc catgccggaa
     5521 agcttcgccc agctctatga cgccttgcgg cggttgccgg cattcctcgc cggcttcgcg
     5581 atcctggcga tgttctggca ggcgcaccat cgcttcagcc gtcgcttcgg gctggaggac
     5641 accaccatcg tgctcgccag catgctgctg gtcgccgcca cgctgttcta cgtgtatccg
     5701 ttgcggatgg tgatgtcggc ggccatgggg ttcttcaccg gcggctgggc gcccagcgaa
     5761 ttcaaggtgc agagcttcct ggagttccgc ctgatcttcg tgatctacgg cgtcggcttc
     5821 gcgatcctgg cggcgatcat cgcgtggatg aacgtgcatg cgctgcgcaa ggcggctgtg
     5881 ttgcagctcg atgccgccga gcgcctggcc gcggaatccg aggtggcatt gaacctggtg
     5941 ctgatcgcct gcgccgtgct ctctgtcgcg gtggcgctgg tgattccccc cagcgtgggg
     6001 tggatgcagg cgcttcccgg gtccgtgtac gcgttgctgg gcatcgcgat gccggtgctg
     6061 agcatccgct atggcaggcg catccaggca ttggccgcgg cgcgggacgc cgcgccgtga
     6121 tcgctgcgct ggagcgcggc gacaaggcca cccgggtgga ggctttcgtc gatgccgcct
     6181 tcgcattcgc gttgaccctg ctggtgatcg ccggcgaccg catccccggc agcgtggagg
     6241 aattgctgca ggcgctgaag agcctgcctg cgtacgcgct cggcttcctg ctcatgctca
     6301 agttctggac cgcgcacgtc gcctggagcc ggcgctacgg gctggacgat gcgaccacgc
     6361 gccggctcag cctgctgctg gtgttcctgg tgctggtgtt cgtgtatccg atgaagatgg
     6421 tgttcggtgc cctgtgttcg gcgctgacct tcggctggtt gccggccaac ttcgtgatca
     6481 ccagcctgcg cgaggtgccc gtgttgttca tcgtgttcgg catcgcgttc ggatcgcttg
     6541 gcatggtgat gtggctgctg tacgcgcacg cctggcgcag gcgcgacgag atcggcctca
     6601 gcgcccgcga acgcatcgac acgcaggtca agctgtggac atgggcgctg gtcccagtgg
     6661 tggcgttgtt gtcgatcgcc tccgcgctgc tgattccggc ccggcccggc atcggctggt
     6721 ggctgggctt tcccggcttc ctctatttca gcctcaatat cgccacgcca ttgctggatg
     6781 cgtggtcgcg gcgcctgcag gcgcgcatgc cgatcgaagg gggcgcgtga tgttctcggt
     6841 ctatatcagc ggcaccgata ccgggatcgg caagacactg gcgagcagtg cgctgctgca
     6901 tgcgctgcgc ggccacggcc tgcgtgcgat cggcatgaag ccggtggcca gcggttgcga
     6961 ttgcatcgac ggcgagtgga aaaacgccga tgccctggca ctgcaggcgg caggccagcc
     7021 cggcatcgcc tacgcggaca tcaatccatt cgcgctggag catccgctcg cccccgaact
     7081 ggccgcgcgc gatgccggca tcgaggtgtc cttgccgccg atcctcgccg cgcatgctcg
     7141 gctcgcggca caggccgacg ccttgatcgt cgaaggcgtg ggtggttggg ccgcgccgtt
     7201 gtcggcatcg ctgatgcagg cggacctggt gcgtgcgttg cgcgtgccgg tgctgctggt
     7261 ggttggcctg cggttgggct gcatcaacca tgcgctgctc agcgcacgtg cgattgccgc
     7321 cgatggcgtg cacctggcgg gctggatcgc ctcgcacgtg gatcccgaga tggagcgcgt
     7381 ggacgacaac atcgcgatgc tgcgcgcgcg catgccggcg ccatgctggg gcgtgttgcc
     7441 gcatgtgccg gctgcggatc ccgcgcggct tgcgtcgctg ttgcgaattc cgatgctggc
     7501 ggaatgactc gcagcagcct tgccttggcg gggtagcgtt cgccgcttag gctccgcatc
     7561 cggctccgat gcgcggccgc gcgccatcca tggcgcgctc gaacgctacc ccgccaagac
     7621 aagactgccc acgcagcgtt gcggctgaaa acgaaaaacg cccggttttc caggcgtttt
     7681 cgttgcagcg ataccgcacg atcaaccgtg ctgcggttcc ggcggcggca aggatgccgc
     7741 ctcgccttcg gccgccttct ggtggtccgc atcgtgcgca tggtcgcggg ccaggtacag
     7801 gtagaacgcc ggcagcacga acagggtgaa cagcgtgccg atggtcatgc ccgacacgat
     7861 caccacgccc atcgagaagc gcgaggccgc gccagggccc ttggcgatca gcagcggcac
     7921 catcgcgaac accagcgccg cggtggtcat cagcaccggg cgcaggcgga tgccggtggc
     7981 ttcctcgatc gcctcgcgct tggacatgcc ctgttcctgc agcttgttgg cgaactccac
     8041 gatcaggatg ccgtgcttgg aaatcacgcc gaccagcgtc accaggccga cctgggtgta
     8101 gatgttgatg ctcatccccg ggaacgcctc gatgccgctg aagctcaaga cgttggccac
     8161 cagcgccagc acgttgagcg ccagcagcgc gccgcagatc gccatcggca cggtgagcag
     8221 catgatcagc gcatcgcgga agctctcgaa ctgcgcggag agcaccagga agatcacgat
     8281 caacgcaagc gccagggtca gcagcatcgc ctggccttcc tgcttgaact ggcgcgattc
     8341 gccggcgtag tccaccgaat agccctgcgg cagcacgtcc ttggcggcct ggtcgaggat
     8401 cgccagcgcc tcgcccttgc tcacgccggg gcgcggcgcg aaggtgaggg cgaccgcgtt
     8461 gagctgctgg aagcgcttca acgattgcgg ctgggtggtt tcgctcaggg agacgatggt
     8521 ggacaacggg atcagttcgc cgctgcgggt gcgcgtgtag tagttgtcca gcgcgctggc
     8581 gttgaggcgg tcgctgcgct gcacctgcgg gatcaccagg tacgagcggt tctgcatcgc
     8641 gaagcggttg gagtagccgc cggacagcat cgccgccatg tccgcggcca gcgtgcgcat
     8701 gtcgatgccc agcgtggctg ccttgtcgcg atccacgttg acctggatct gcggcttgtc
     8761 gatcttcagg tccttgtcca ggaagatgaa gcgcttgctg gccatcgcct tcgccaggat
     8821 ctggtcggcc acatcggcca gctggctcag gtcgcccacg ccgccgatga tgaactcacc
     8881 gccgccggca ccgccgcccg cgctgggcag cgatggcggg accagcgcga acacgttgag
     8941 gccggtgacc tgcgacatct tcggctgcag gtcctgctgc aggacctggt tggtcgagcg
     9001 cgtgcgctcg ctccacggct tcagcacgaa gccggccatc gccatcgggc tggcaccacc
     9061 accgctgccg ccgaagccgc cgttgaacag gaagtagtcc tgcacctcgg gcacgccctt
     9121 caccatccgg gcgatctcgt cggtgtagcg ctccacgtag tcgagcgtcg ccgtgggatc
     9181 cgcgctggcc accgagaaga tgaagccttc gtcttccagc ggcgcctgtt ccttcggcgc
     9241 gctggtgtag aggaagccgc agctcaccag cacgatggcg ccgaacaccg cgatcaccgc
     9301 cttggtgtcc agtgcgccgt gcagcttgcg ctggtagccg tggcgcagcg tctcgaactt
     9361 gctgtccagc cacagttcca gcttgccctt gggatggtcc ggatcgtgcg cgatcaggat
     9421 ccgcgaactc atcatcggcg tgagggtcag tgcgatcacg ccggacagca gcacggcgcc
     9481 ggccagggtg aacgcgaact cggtgaacag cacgccggtg aggccgccct ggaagccgat
     9541 cggcaggtac accgcgatca gcgtggtggt catcgccacc accggccacg ccagttcgcg
     9601 cgcgccgcgg atcgaggcgt cgaacggcgt catgccttcc tcgatatgcc ggtggatgtt
     9661 ctccagcacg atgatcgcgt cgtctacgac gatcccgatc gcaagcacca tcgccagcaa
     9721 agtgagcagg ttgatggtga agcccatcag cagcatcagg aacagcgcgc cgatcagcga
     9781 cagcgggatc gtcaccgccg ggatcagcac actgcgcatc gaccccagga acaggaagat
     9841 caccacgatc acgatgatca gcgcctcgat gatcgtgctg atgacctcgt tgatcgcgtc
     9901 ctggatcgcc tcggtgctgt cgtaggggat ggtcgccttg atgccctgcg gcagctgcgg
     9961 gacgatttcg ccatcccaca ccttgcgcac gtccttgatg acgtccagcg agttggcgtc
    10021 gggggcgacg aagataccca tgaaggtcgc ggcttcgccg ttgatgcgca ccgaggtgcc
    10081 gtagctctcg gagcccagca ccacgtcggc gatgtcgccc aggcgcacga ttgcgccgtc
    10141 ctgttcgcgc accaccagct tgcggaattc ctcggcgttg cgcaggtcgg tgcgcgcggt
    10201 catgtcgatc gcgaccatct ggcccttggt ggatccgacc gcggccagca cgttgttcga
    10261 tgacagcgcg ttggacacgt cgctggcggt gaccttgtac gcggtcatgc ggtccggctt
    10321 cagccagatg cgcatggcga aggtgccggc gccgaggatg tccgcgcgct gaaccccgtc
    10381 gatggtgacc agcttcggct ggatgacgcg ggtgatgtag tcggtgatct ggttgttgtc
    10441 cagggtctcg ctggccagcg agacgtacat cgccgcgatc tgctggccct gctgcaggtc
    10501 gatcaccggt gcttccgact cggccggcaa ctggccgcgc atcttgttga ccttggccga
    10561 gatctgggtg agcgcctcgc tcggatcctt gtccagctgg atgtaggcct ggatcgtgct
    10621 gatgccggcc gcgctggtcg aggtcaggta gtcgatgccc tccgcgctgg cgatctcgcg
    10681 ttccagcggt gtggtgatga agccctggat caggtcggcg tcggcaccga agtacgtggt
    10741 gctgatgttg accaccgcat tgcgcatctc cgggtactgg cgcacgttca gttcgctgaa
    10801 ggcgcgcagg ccgaacagca ggatgaacag gctgacgacc agcgccagca ccggtttccg
    10861 gatgaagaga tcggtgaatt tcatgctttt gttctcccgc tcggccgcgt gcggcgaggg
    10921 tcggtgggac gcccggcgct ccaccgggcg ttcgacgatt caggcgctgc ggtcaaccct
    10981 ggcgcatcac cggttttccg gcgtcgggct tgcgtccgcg gacggctgga tggcgtcgtt
    11041 gatggtgact tcggcatcgt tgcgcagctt cagcaggccg ctggtgacga tctgctcgcc
    11101 ggccttcagg ccctcggtga ccacgatcag gtcgccctgg gtcgcgccgg tcttgatgaa
    11161 gcgctgggtg acgatgaact tgtcgccggt gagctccttg ccctgcatgt ccttctcgcc
    11221 cggcttgcgc gcggtcttgc tgatcacgaa caccgcattg ccgtacgggt tgaagctcac
    11281 cgcggtttgc ggaatcacca gcacatcgcg cacaccgccc agggcgaacg tgaccttggc
    11341 gaaactgccg gggcgcagcg cgccgtccgg gttcgcgaag gtcgcctgcg ccttgaagtt
    11401 gcgcgtgctg gcatcgacca gcggctcgac cgcggtcacc cggccctcga acgcctggcc
    11461 cggcagcgca tccacgctgg cgcgcaccgc ggtgccttcg atcatcttgc cgatctgctg
    11521 ctccggcagg ctgaagtcca ggtagatcgg atccagttgc tgcaggctga ccatcgccgc
    11581 gcccggcgcc acgtactggc ccaggttgac cttgcgcagg cccagcacgc cggtgaacgg
    11641 cgcgcggatg gtcttctgcg cgatcagcgc gcgctgcgcc tcgacctgcg cgcgtgcgct
    11701 cgctgccgcc gcgacgcgct gctgcacgtc gtccagcgag accagcttgt ccttgcccag
    11761 cttctgccag cgctcggcct gcacgttggc cagcttggcg gaggtttcca gcgcgttgag
    11821 cgtggcttcc tcgttggcgg tgttcaggcg gaacagcacg gtgccggcgg agacgcgctg
    11881 tcccggttcg aactcgatcg cgcggatcac gccacccgcc tcggtggtga tgtcggtgcc
    11941 gttgatcgcg acgaaggtgc ccaccgcctc gccgttgtcg gtccagcgct gggtcttggc
    12001 gacgtaggaa ctcaccgcca ctgccggctg cggcatgttg tcgaagaagt tgttggtctg
    12061 cttggcgaag aacgccttga tcgcgaacac gccgccgaac accacggcga cgccgaccag
    12121 catcaggacc cagcgaaggg tggtgttggg cttgcgcttg gcggcggctt tcgacatggg
    12181 gaggcctcgg atcttggcga tattgaactg ttcagtgcaa tattgtgccc gtgaatgcga
    12241 tcaggacgca atcgtgactg acggcacggt tgtacctgca cgtgcatcag cgacgcgtgg
    12301 cggccgcgca tttcgacatc gatcggccga ttaaagtacg cacgcaaacg gggacaggag
    12361 caacacgatg gggacacagt ggtgtcgcac atggcgagac gcgggcaacc ggtgggcgct
    12421 gctcatcgcg ctggccggca tggccgtgat atcgaacgtc gctaccgccg cgccgcccgc
    12481 acaacctgat ccggactgcg tgggcgtggt gctgggcgga ggcggtgcgc gcggcatcgc
    12541 ccatatcggc gtgctgaaag tgctggagcg cgagcgcatc ccggtctgcg cagtcaccgg
    12601 caccagcatg ggcgccatcg ttggcggcct ttacgccacc ggctacgacg cggaggagct
    12661 ggagaagatc gtcggcgcca tcgactgggc tgacatgttc gtcgacgacc cgccgcggca
    12721 ggaactgccg atggagcgca aggacgagga tttccgccat ctgctcgacc tcgagatcgg
    12781 ctacaaggac gggcgcctgg gcttcccggt cggcttggtg cgcgggcaga agctcatgct
    12841 gctgttgcgc cgcctgaccc tgtcgacctg gcgttcggag gatttcgacg cgctgccgat
    12901 tccgttccgc gcggtctccg ccgacatcgt caccggcagg aaagtggtct tcgatgacgg
    12961 cgacctggcc ctggcgatcc gcgccagcat gtcggtgccg ggtgcgttcg cgccggtcaa
    13021 ggtcggcgac cagttgctgg tcgatggcgg catggccgag aacgtgccga tcgacgagat
    13081 gcgcgcgatg ggcgtgtcgc ggatgatcgt ggtcgacgtc ggctcgaagc tgctcggcga
    13141 gaaggacctg accaatcccg cggtcatcct cgaccagatg atcaccgcgc tgatgaccga
    13201 gaagaccgag cgcagcctgg ccacgctcgg tgccggcgac gtgctggtcc gccccgagct
    13261 gggcgacatc acgtccggcc agttcaatcg cacggcggag gccgtggcgg tcggcgaacg
    13321 cgccgccgag gcgatgctgc cgcagctgcg tcgtcatgcg gtatcggcgg aacgctacgc
    13381 cgccctgcgc gcgcgccagc atcgccgcga cttcgatccc ggccttgtcg ccttcctcga
    13441 cgtggagccc gggcatacgc cctcggcgac gcggcacgtg gcctgggcca ccgaagggct
    13501 ggccggcaag cccttcgacg tggacaaggt ggaacacgcc gtcggccgcg cctatggcga
    13561 tggccgcttc gagcagatcg gctaccgctt ggtcgaacgg gacggacagg ccggcctgga
    13621 agtcatcccc acgcagaagc cgtggacggc gttcggccgc atcggcctgc aactggacga
    13681 caacttcaac ggccgcaaca actacctgct gtcggcggaa ctcaccttca acgatgccaa
    13741 ccgcagcggc ggcaagtggc gcaacctgct gcagctcggg cgggtgaccg ggttgcgctc
    13801 ggaattcgtg cagccgttcg gccgggccgg cgcgttctac ctgaagcctt cgctggagat
    13861 ccgcgctgat tctctgccac tgtcggtgga tggcgagcag gtggcggagt accggctggt
    13921 gcgccgcggg ctggacatcg aggcgggcta ttcgccatcg ccagaatggc ggatcgggat
    13981 agcggcaatc ggcggccggg atcgtgcgcg acgcatcgtg ggtgactcca gcaccttcgc
    14041 cggtggatcc gaaaccttcg ccggcgtgcg cgtcaacgcg acctgggatt cactggacag
    14101 cgtcagcttc cccaccaagg gcctgcgcgc cactgccgaa ctcgtatccc tgcatccgtg
    14161 gggcaccacc attctggatg gcgaggtcgc gcgggcatcg ctggactggg cctattcccg
    14221 cgatcgctac cacctgctgc tgggcatgcg cctggccagc gcgctgcagg atgacggctt
    14281 cttccaggcg cagaccttcc tgggcggctt cctcaacctc tcgggcttcg gcgaacgctc
    14341 gctggtgggg aaccaggcgg cgctggcacg cgccgtggtc tatcgccgcg tgggcgacac
    14401 cacgcgcatc ttcgccatgc cgatgttcgt cggcggcagc ctggaggccg gcaacgcctg
    14461 ggatcgccgc gacgacgtgg atttcggttc gctgctggtc ggcggcagcc tgttcaccgg
    14521 cttcgacacc ccgctgggcc cgatcttcct gggctatgga cgcaaccagg ccggtgccga
    14581 ttcctggtac ctcaccttcg gctcgctgct gcggcaggag ccgaagtgat ccccctcggc
    14641 aggcatccgc cgaggcaata aaaaacccgg cagacgggct gccgggtggg gggtcgcagg
    14701 gcccgaggga gggagcgtgc cctgcgatgt cgcgcgtgat gcgattactt cttcgaagcg
    14761 gccttggtga ccttctcgac ttcggcggtc gcctgcgcgg tggccttctc gaactggccc
    14821 ttggccagcg cgccgatggc ttcgttggtc ttcacggtgt tggcgaacac ttcctgcgcg
    14881 gtgcccaggc tgcgctcggt ggcggtgcgg gcgatctgga cgcccttcgg catcaccgac
    14941 ttgaagcctt cgacgtcacg gacttcgatg agctcgttcc agaacgcgaa ggcggcgctg
    15001 gtgttctctt cgaacgcagc catctgcagg ccgaacgcct tctgggcgtt gtcgatggcg
    15061 aggcggttgg cgttggcggc aacgtcggcg aactggctgg tcgccttggt gaactggtcg
    15121 ttgaactgat acatggcgga ttcctggctg gtcacgccgg cagggattgc cggtttgtgc
    15181 ggtgcaccat atcgccgatc atgttgcggt gcaacatcag cccgacgaac ggcagctcgt
    15241 cgttcaggat cgagccaatc gcccgctgcc catgtgtcag cccgtaccgc cgcggcgatt
    15301 accccacaag gcgggccgcc ccggcgtcgc gccggcgtca tcgaccggat gcggcacggc
    15361 gtcgaggccc gctaccgcgc gcgcgaacgg ggcgaggtgg ctgagcgcac gcgcatacac
    15421 gctgcgcttg aaggtcacca cgtgcgccat cgggtaccag aaatcgaccc agcgccagct
    15481 gtcgaattcc ggtttgtcgg tcaggtccag cttcaggtcg tcgtccctgc cgcggaaccg
    15541 gagcaggaac cacacctgtt tctggccgat gcagaccagc cgatcctgcc ggcgtaccgc
    15601 ccgcgccggc aaccggtagc gcagccagcc gggcgtgacc ccgagtacgt cgacgtgttc
    15661 cggcagcagg ccggtttcct cgcgcagttc gcgatacatc gcctcgagcg gggtctcgtc
    15721 ggtattcatc ccgccctgcg ggaattgcca gccgtcccgg tgcacgcgac gtgcccagaa
    15781 cagccgtcca tcctcgtgca tcagcacgat gcccacgttg gggcgaaacc cgtccggatc
    15841 gatcacgatg cggactcccg aaatgccggg gcaatgccct gatgcttgga ctctgccagc
    15901 gtggcaggcg gggggcaagc catccggtca ggattgacgg gaccgggccc ggcatgaata
    15961 attccgggtt ctgcgccggc tatgtagctc agccggttag agcacagcac tcataatgct
    16021 ggggtcggtg gttcgagtcc acccatagcc accacggcgc agcacgcaac cccgcttccg
    16081 gcggggtttt tcgtttctct tccc
//
LOCUS       DAOOCX010000046        15935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3305_length_15935_cov_8.764169, whole genome shotgun sequence.
ACCESSION   DAOOCX010000046 DAOOCX010000000
VERSION     DAOOCX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15935
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3305_length_15935_cov_8.764169"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /gene="glnA"
                     /locus_tag="PK399_06130"
     CDS             complement(<1..1135)
                     /gene="glnA"
                     /locus_tag="PK399_06130"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPW12728.1"
                     /translation="MSLENVEKLIKDHKVEFVDLRFTDVRGVQHHVTFPKSIVDASLF
                     EDGKMFDGSSISGWKGINESDMVLLPDASTAIIDPFTADPTLILTCDILDPATMQAYA
                     RDPRGVAKRAEAYLKSSGIADTAFFGPEPEFFIFDSVRFANEMGHTFFHVDSEEAAWN
                     SGREYEGGNTGYRPGVKGGYFPVAPLDSLHDIRAEMCKELEAIGIEVEVHHHEVANAG
                     QCEIGTKFNTLVQKADELLSMKYIIKNVAHRNGKTATFMPKPIVGDNGSGMHVHMSLA
                     KDGKNLFSGDGYGGLSQMALWYIGGIFKHARAINAFANSTTNSYKRLVPGYEAPVMLA
                     YSARNRSASCRIPFVTNPKARRIEIRFPDPMNSGYLTFAALMMA"
     gene            1345..2199
                     /locus_tag="PK399_06135"
     CDS             1345..2199
                     /locus_tag="PK399_06135"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPW12729.1"
                     /translation="MNDLLAALLLGIIEGITEFLPISSTGHLLIAERWLGHRSDLFNV
                     AIQAGAILAVVLIYRRRLLGLLAAFAGRDMTAPYDPLGIDISPKLAREYGFKLLVAFG
                     VTAVGGLAVKALGWELPDTVRPIAWALIIGAVWMVIAEQLAARRAARDGERTAISWTT
                     AILVGVAQVVAGVFPGTSRSAATIFVALLAGTTSRSAATEFAFLVGIPTMFAATGYEL
                     LSMARHGGLHGEDWSALGVAFVASAITAFVAVKWLLRYIETHRFTAFAVYRLLLGIAL
                     LLWVPSGG"
     gene            2257..3087
                     /locus_tag="PK399_06140"
     CDS             2257..3087
                     /locus_tag="PK399_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META and DUF4377 domain-containing protein"
                     /protein_id="HPW12730.1"
                     /translation="MRISSLALLLPIALAACTGTMSTPGPAAPAAPEPPAPPAAPAAP
                     EIAAELPRFHWRLQEATTADGTRIEALFARADKPVQLDFDKGRLAVSNTCNRMGGSYA
                     IAGGTLTVGRMASTMMACADAKLMALDGEVGKRLEGALSLALVAGETPQLTLGNTVGD
                     RLVFAGTPTAATRYGGPGERMFLEVAAQTKPCSHPLIPDMQCLQVRELAYDDHGLKVG
                     TPGAFGNFYDSIDGYTHEPGVRNVLRVQRYTRRDVPADASKYAYVLDMVVESDASATP
                     "
     gene            complement(3091..3699)
                     /locus_tag="PK399_06145"
     CDS             complement(3091..3699)
                     /locus_tag="PK399_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HPW12731.1"
                     /translation="MKVHSDSFEHGKPIPSEFAMGARDGANGFGGNRNPHLAWREVPD
                     GTQSFALLCIDTDAPTDGALVANASIDIPVAHPRGDFVHWTVIDIPADRNEIAAGSCS
                     DGITPRGKPAGSDEGGRRGLNDYTGWFAGDAAMGGDYFGYDGPYPPPHDARVHRYFFR
                     LFALDVATLDLPAAFTAGDVFRAMHGHVLAEASTYGTYSLKR"
     gene            complement(3883..>4723)
                     /locus_tag="PK399_06150"
     CDS             complement(3883..>4723)
                     /locus_tag="PK399_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPW12732.1"
                     /translation="GIRLHGVYSTLPARAPRGLALLLHGWEGSAESGYMRLTCARLLE
                     RGFDVFRLNFRDHGDTHHLNEGLFHSNRLDEVLQAALAVSRRWQRPGQPMVVAGYSLG
                     GNFALRVALHAPRMGLQLARVAAVCPALDPERTTIAMERGLPVYERYFLKKWTRSLRR
                     KRELFPERHAIAEAQLRSRRLRELTAWLVARHTGFADVDAYFDGYRISRDRMRGLQVP
                     ADILTSADDPVIPVEDFHDWQLPADATVEIAAHGGHCAFLRGASLRGYAEDWVAERLS
                     VVL"
     assembly_gap    4724..4823
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4824..5484)
                     /locus_tag="PK399_06155"
     CDS             complement(<4824..5484)
                     /locus_tag="PK399_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW12733.1"
                     /translation="MPATSPAVEFFLRALRYAYRLPFLLWHVFVHLPVLLVLIAIGDD
                     GRRPIGHAAIRLWARMLLRVFGMRVQGMGTPLPGGTLFVANHISWVDIVALHSQHMMG
                     FIAKSEIRGWPVVGWITSHAETIYLQRGNANSLGDVMAEMSARLRSGRAVAAFPEGGT
                     RDGRELGAFHARIFAAAVEADAPVQPVALCYGEGCEAQPIVAFARGESFMANFFRLLG
                     EP"
     gene            complement(5535..5909)
                     /locus_tag="PK399_06160"
     CDS             complement(5535..5909)
                     /locus_tag="PK399_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HPW12734.1"
                     /translation="MEALGIRFTELGDGFLRGTMPVDARTHQPYGLLHGGASVALAET
                     LGSSAGMLAARGDAVVGLEINANHLRAVRSGLVTGTARPLHVGRSTQVWEIRIEDAAG
                     KPVCISRITLAVLPKPPASGQA"
     gene            complement(5992..6630)
                     /locus_tag="PK399_06165"
     CDS             complement(5992..6630)
                     /locus_tag="PK399_06165"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HPW12735.1"
                     /translation="MSITLNFELNDRDLAHFQEAAERSRKAAEGKSEQEIVDCAVGLL
                     ADAQKVHIPDFIKERLLRLDDMIAMVRDTGWALPDDDKQRVLSALTYFCDPNDVIPDH
                     VEVLGFLDDAVMIELSMRELKHELDAYDDFCDYRANEASRRGVEPATLGRTDWLDGRR
                     DELIERMHQRRERDIGGGYGRSSGYGGSGGYGAPGGSKSYTRSWRPGGFKFG"
     gene            complement(6745..8673)
                     /locus_tag="PK399_06170"
     CDS             complement(6745..8673)
                     /locus_tag="PK399_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HPW12736.1"
                     /translation="MLAGACALLLAGCATLSVEQRDEAAGIARAARSSQVDCTRADAC
                     ALASPLRALGRQALAASTPAAPRHVALILDDAPDALLVRIHLIRSAQRSIDLQTYIFD
                     EDDSAQLVLDELQAAAFRGVKVRVLVDQLAALRQVDTLAGLASMHANFELRLYNPVLD
                     RARLALPMYAVAAACCWRQLNSRMHNKLLLVDGEVGITGGRNYQDDYYDWGAGYNFRD
                     RDLLVAGPVAGEMATNFEAFWSSPRSVPAEQLGDVARLLQKQGAPPPPHAPYRKPERV
                     RALLAMVDDDAAIRARFVDRAMPVQGVQFIADLPVKHWQGRPAEADAAAEAASSGLRT
                     LIAGAHDEVLLQTPYLVLSKPAQHLFRELRRQDDPPRVRISTNSLASTDAFIAYAISH
                     KYKRRYLRDFGFEIHEFKPFPADAPFEPGALGADLPGFTDTAASPALPASAGARPTLR
                     ERLLAKRGLRKDPDSEAGRFRSIGSGGMPVRLKQAGMRMGLHAKSMVVDGRVGVVGTH
                     NFDPRGDTYNTESAVVIEDPAFAARLAASIQRDMAPGNAWTIGRRDSSPILPGVEYTL
                     ARISERLPIFDLWPIKYATSYEFVPGPECPTPPSPFDPRFRACHTPVGNSPEVDIGLK
                     WLGVRVFTAFGSGLSPIL"
     gene            complement(8769..9242)
                     /locus_tag="PK399_06175"
     CDS             complement(8769..9242)
                     /locus_tag="PK399_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPW12737.1"
                     /translation="MRELILLRHAHAESATAGQDDIARPLSATGLAEARAAGDWLREH
                     GLRPDRVLCSTATRTRDTLAALGDIGGAEVHEDASIYDASPGTLVALADANRDAERLL
                     LVGHNPGLEQLAALVHSGQTGDYRGMPPGGIAVLRLPMDVNIEPGIATLTQFWWP"
     gene            complement(9270..9899)
                     /locus_tag="PK399_06180"
     CDS             complement(9270..9899)
                     /locus_tag="PK399_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPW12738.1"
                     /translation="MKTWLVACSKGGVGKTTIATQLAANAALSGLRTVLVDADPQGSA
                     TRWCERRAGLASAVLPLDGTRKAWRKRVPDDAQRLIVDAPAGAMADDLDEVLETADAV
                     IVPVQPSALDIEASVRFLESLGRHPRVRKGKLRVGLVGNRLKPNTNASQQAMDVLRSW
                     PFPLVAQLRDSQAYVVLTGLGKSLFDYHSAQVRDHQADWQPLLHWLKKD"
     gene            complement(9965..>10402)
                     /locus_tag="PK399_06185"
     CDS             complement(9965..>10402)
                     /locus_tag="PK399_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPW12739.1"
                     /translation="GSGMVGAEQGARIQSANNRQAVATEAGLPQLEDPPTLQVSVLED
                     LIGGNVGEARALLEDFLGSTDEDLAVLEQLRGAGDLHGLTRQAHKVKGAARLVGALEL
                     AEAAAALEGAGRGGDWASVLPLAVDVHTAAARLRLDVAQRYPA"
     assembly_gap    10403..10502
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10503..13038)
                     /locus_tag="PK399_06190"
     CDS             complement(<10503..13038)
                     /locus_tag="PK399_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW12740.1"
                     /translation="MRFDLSRFGLRHQLLGLFGLFLITCALVLSIDEIGQHYTQRSLA
                     AMKDDVLAGMGRIRRLTDAYAQDVVDTTFRTRNHLLDWDEGVAVVTAARNSAAQEWHA
                     LQALPLEGEDKALLDQALQARVRADQAAETLQRILQAKDIRALGQFADRELYPAVDPL
                     VERLDRISEHGQLRAEALVQAELTRSKWVGRIRIVLSLLCFLLVALFGRRILRNGYRG
                     VESLHGLARRMAQHDYTAEPHYTPSGELGEVMDSFLRMRNHVRRIETQLTDQLASNDR
                     VREALERREHFQRLLLEAAQTAIFAVDEEGVFSQVNPFGEQLLGWPPGSLLGRESMDA
                     ILDPEALQSLARSLSEAYGQRIAPDWTALRALAQHREPPREFVLRHQRGRTLPVLLAL
                     SAMRDDTGAMVGLLAVATDLTVLKRLERALRDSETRARDANHAKSAFLAAMSHEIRTP
                     MIGVTGMIEVLSHTDLDIEQRRSLNVIQASAEALLRIIGDILDFSKIEAGRMEIEPVP
                     TSLPDLLRSVVANYAGSASSKGLALSCQVDPRIAPAHYADPLRIRQVVGNFVSNAIKF
                     TEHGGVTVAVEPGRHDPADGALGSDALVIRVTDTGIGISEQAQARLFQPFSQAEVDTT
                     RRFGGTGLGLAISRRIGELMGGRVDMESTPGAGTTMRLALTLQRAPPDELPDLLLPGK
                     PVAGFTPRRLPTVSEAEAERSLVLLVDDHATNRQVIQRQLALAGYASESAEDGVEGLE
                     RWRSGRYALLLSDVHMPRMDGYQLARAIREEAARRGLPPTPIVALTASALKGEAERCL
                     AAGMDDYLAKPVGIATLGACLQRWLPHTAGSGMVGAEQGARIQSANN"
     gene            complement(13081..14277)
                     /locus_tag="PK399_06195"
     CDS             complement(13081..14277)
                     /locus_tag="PK399_06195"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HPW12741.1"
                     /translation="MSGSELQVMVVEDHGFQRRIALRLLSELGIERVLEGADGLHALE
                     VLRKQATPPDVVLVDLDMPGMDGIECIGHIAQERLARAVVVVSALDPALLNTVQTMAR
                     AYGLRVLGSVEKPLTRDKLEATLNRFEDNAGEEQDDGEADFSLQALADALERGEIVPW
                     FQPQVEFGNGKVIGVEALARWERPDGSLVRPNLFVPMLEREGLANKLTDRMLDAACRW
                     KQGWDQHGHRLKLSVNVSAASLADPSAADRYQEIVESYGVEPEDVILEITESSVMSDA
                     ARGLGLLARLRLKGFGLSIDDFGTGYSSLAQLSQIPFTELKIDQGFVFGAHAQPRKRA
                     VVEASLDLARKLGLTAVAEGVEDMEDWQMLAELGCDIAQGYLIGRPVPGSELQAAIAR
                     WRRPAH"
     gene            complement(14374..>15935)
                     /locus_tag="PK399_06200"
     CDS             complement(14374..>15935)
                     /locus_tag="PK399_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPW12742.1"
                     /translation="IPEVVAHAASLHIDVVPEVDIPGHATAAISAYPQLGVSGNPIAV
                     SNEWGVNVNLFNADDATMRFLEDVMGQLVNLFPGRYVHIGGDEAVKDQWKASPKMQAR
                     IRELGVKDEAGLQAWMVARLERYLSANDRRLIGWDEILDGDLPESAAVMSWRGTEGGI
                     AAAKRGHDVVMAPWDKLYLDYLQTMSPTEEPGRPTQVTLETVYAFEPVPAVLDATQAK
                     HILGVQANVWTEHMRSFARVQHAVFPRIAALAESAWSSREGKDYAGFLQRLPLLLPRW
                     DARGIAYAKTPFQPLVDVAQADARGKATVTLSQPLGYAIRYTTDGSTPTARSPLYAQP
                     LSLALPATLQAGAFFDGVALAPPVARELSAASLLSRSDEELAMCTGQLMLRLEDDGPR
                     DGARAIYNVDIFNPCWSWKQAPLAGIGAVEVRAGRIPYFFQLAHDEPARTFKPAKTAH
                     GELQLHAGCDGPLLATVPLPETTDADGFVTLRAPLPDAPASTDLCLWFTGDTRPAMWV
                     LDRATLVPATP"
BASE COUNT         2381 a         5508 c         5378 g         2468 t
ORIGIN      
        1 cggccatcat cagcgcggcg aaggtgaggt agccggagtt catcggatcc gggaaacgga
       61 tctcgatgcg acgcgccttc gggttggtga cgaagggaat gcggcagctc gctgagcggt
      121 tgcgcgccga ataggccaac atcaccggcg cttcgtagcc cggcaccagg cgcttgtagc
      181 tgttggtggt ggaattggcg aaggcgttga tggcgcgggc gtgcttgaag atgccgccga
      241 tgtaccacag cgccatctgc gacaggccac cgtagccgtc gccggagaac aggttcttgc
      301 cgtccttggc cagcgacatg tgcacgtgca tgccgctgcc gttgtcgccg acgatcggct
      361 tcggcatgaa ggtcgcggtc ttgccgttgc ggtgggcgac gttcttgatg atgtacttca
      421 tcgacagcag ttcgtcggcc ttctgcacca gggtgttgaa cttggtgccg atctcgcact
      481 ggccggcgtt cgccacttcg tggtggtgca cttccacttc gatgccgatg gcttcgagtt
      541 ccttgcacat ctcggcgcgg atgtcgtgca gcgaatccag cggcgcgacc gggaagtagc
      601 cgcccttcac gcccggacgg tagccggtgt tgccgccttc gtattcgcgg ccggagttcc
      661 atgccgcttc ttccgagtcg acgtggaaga aggtgtggcc catctcgttg gcgaaacgga
      721 cgctgtcgaa gatgaagaat tccggctccg ggccgaagaa cgcggtgtcg gcgatgccgc
      781 tggacttcag gtaggcctcg gcgcgcttgg ccacgccgcg cgggtcacgc gcataggcct
      841 gcatggtcgc cgggtccagg atgtcgcagg tcaggatcag cgtcgggtcg gcggtgaacg
      901 gatcgatgat cgcggtcgac gcgtccggca acaggaccat gtcggattcg ttgatgccct
      961 tccagccgct gatcgaggag ccgtcgaaca tcttgccgtc ctcgaacagg ctggcgtcga
     1021 cgatcgactt cgggaaggtc acgtggtgct ggacgccacg cacgtcggtg aaacgcaggt
     1081 cgacgaattc gaccttgtgg tccttgatca gcttttcgac attctcgagc gacacggcag
     1141 gctccgatac aggcggttgg gataaccggg atgtgggttg acatcgcaag cgccgtgcca
     1201 actttcaacc cattgatttt caatggattg ctgcaatgca gccaacgcgt tagcactgct
     1261 tcggttcaat tttcgctatc gatgcacttc tttggtgcga atgcgcctgc gttatcctgc
     1321 gccaccaccg actgcacgcc acccatgaac gacctgctcg ccgccctgct gctcggcatc
     1381 atcgaaggca tcaccgaatt cctgccgatc tccagcaccg gccacctgct gatcgccgaa
     1441 cgctggctgg gccatcggag cgacctgttc aacgtggcca tccaggccgg cgcgatcctg
     1501 gccgtggtgc tgatctaccg ccgccgcctg ctcggcctgc tggccgcgtt cgctggccgc
     1561 gacatgaccg cgccctacga cccgctcggc atcgacatca gtccgaagct ggcgcgcgaa
     1621 tacggcttca agcttctggt cgcattcggc gtgaccgccg tcggtggcct ggcggtcaag
     1681 gcgttgggct gggagttgcc ggacacggtg cggccgatcg catgggcgct gatcatcggg
     1741 gcggtgtgga tggtgatcgc cgaacaactg gcggccaggc gcgccgcgcg ggacggcgaa
     1801 cgcacggcga tttcctggac gaccgcgatc ctggtcggcg tggcccaggt cgtggccggc
     1861 gtgttcccgg ggacctcgcg cagcgctgcg acgatcttcg tcgccttgct ggccggcacc
     1921 accagccggt cggcggcgac cgagttcgcc ttcctggtcg gcatcccgac gatgttcgcg
     1981 gcgaccggct acgaactgct gtcgatggcc cgccacggcg gcctgcacgg cgaggactgg
     2041 agcgcgctgg gcgtcgcctt cgtggcctcc gcgatcaccg ccttcgtcgc ggtgaagtgg
     2101 ctgctacgct acatcgagac ccaccgcttc accgccttcg cggtgtatcg cctgctcctc
     2161 ggcatcgccc tgctgctgtg ggtgccgagc gggggataag ccgcacgggt ggatgacgcg
     2221 acgtcgcctc gtcccgtccc tccacggagc ctgtccatgc gtatctcgtc gctcgccctg
     2281 ctgttgccga ttgccctcgc cgcctgcacc ggcacgatgt cgaccccggg acctgcagca
     2341 cccgctgcgc ccgagccgcc ggcgccaccc gctgcgccgg cagcacccga aatcgcggcc
     2401 gagttgccgc gcttccactg gcgcctgcag gaggccacga ccgcggacgg cacccgcatc
     2461 gaagccctgt tcgcgcgcgc cgacaagccc gtgcagctcg acttcgacaa gggccggctg
     2521 gcggtctcga acacctgcaa ccgcatgggc ggcagttacg ccatagccgg cggcacgctc
     2581 accgtcgggc ggatggcttc gaccatgatg gcctgcgccg acgcgaagct gatggcgctg
     2641 gatggcgaag tcggcaagcg cctggaaggc gcgttatcgc tggccctggt cgcgggcgag
     2701 acgccgcaat tgaccttggg gaataccgtg ggcgaccgcc tggtgttcgc cggcacgccc
     2761 accgcggcca cgcgctacgg aggccccggt gagcgcatgt tcctggaggt cgcggcgcag
     2821 accaagcctt gcagccatcc gctgatcccg gacatgcagt gcctgcaggt ccgcgaactc
     2881 gcttacgacg accatggcct gaaggtcggt acccccggcg cgttcgggaa tttctacgac
     2941 agcatcgacg gctacaccca cgaacctggc gtgcgcaacg tgctgcgggt gcagcgctac
     3001 acccgcaggg acgtcccggc cgatgcgtcg aaatacgcct atgtgctgga catggtggtg
     3061 gagtcggacg ccagcgcgac accgtgaccg tcacctcttg aggctgtacg tcccgtaggt
     3121 actcgcctcg gccagcacgt ggccatgcat ggcgcggaac acgtcgccgg cggtgaatgc
     3181 cgccggcagg tccagcgtgg cgacgtccag cgcgaacagg cggaagaagt aacgatgcac
     3241 gcgcgcgtcg tgcggcggcg ggtacggccc gtcatagccg aagtagtcgc cacccatcgc
     3301 cgcatcgccg gcgaaccagc cggtgtagtc gttgaggccg cgacggccgc cctcatcgct
     3361 gccggccggc ttgccgcgcg gggtgatgcc atcgctgcag ctgcctgcgg cgatctcgtt
     3421 cctgtcggcg ggaatgtcga tgacggtcca atgaacgaaa tcgccgcgcg ggtgcgcgac
     3481 cgggatgtcg atgctcgcgt tggcgaccaa tgcgccatcg gtgggcgcgt cggtatcgat
     3541 gcacagcagg gcgaacgatt gcgtgccgtc cggtacctcg cgccatgcga ggtgcggatt
     3601 gcggttgccg ccgaagccgt tggcgccgtc gcgcgcgccc atcgcgaatt cggacgggat
     3661 cggcttgccg tgttcgaagc tgtcgctgtg gactttcata tgggttctcc ggcgggcgga
     3721 gttttgattc tatgacgcgt catgaagaag gcggaaaagc ggaaaagggg ccagagtaaa
     3781 gttttgcggg aaggcctgtt cggcatgcca ttccactaca ccgcaaaatt tactctggcc
     3841 ccttttccgc tggccccttt tccaccagct gcagcgagca actcagagca cgaccgacaa
     3901 gcgctccgcc acccaatcct cggcgtagcc gcgcagactg gcgccacgca ggaaggcaca
     3961 gtggccgcca tgcgcggcga tctccaccgt cgcgtccgcg ggcagctgcc agtcgtggaa
     4021 atcctcgacc gggatcaccg gatcgtcggc cgaggtcagg atgtcggccg gcacctgcaa
     4081 tccgcgcatg cggtcccggc tgatccggta gccatcgaaa tacgcatcga cgtcggcgaa
     4141 cccggtgtgc cgcgccacca gccacgcagt cagttcgcgc aggcgacggc tgcgcagctg
     4201 cgcttcggcg atcgcatggc gctccgggaa cagctcgcgc ttgcggcgca acgagcgcgt
     4261 ccacttcttc aggaagtagc gctcgtagac cggcaggccg cgctccatgg cgatggtcgt
     4321 gcgttcggga tccaacgccg ggcagaccgc cgcgacgcgt gccagctgca agcccatgcg
     4381 cggcgcatgc agggcaacgc gcaaggcgaa attgccgccc agcgaatagc cggcgacgac
     4441 catcggttgc cccggccgct gccagcgccg ggacaccgcc agcgcggcct gcagcacttc
     4501 gtccaggcgg ttggaatgga acaggccctc gttgaggtgg tgggtatcgc cgtggtcgcg
     4561 gaaattcagc cggaacacgt cgaacccgcg ctcgagcagg cgcgcgcagg tcagccgcat
     4621 gtagcccgat tccgcgctgc cctcccagcc atgcagcagc agcgcgaggc cgcgcggcgc
     4681 gcgcgccggc agggtgctgt acacgccgtg caagcggatg ccgnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnncgggttc gccgagcaag cggaagaagt tggccatgaa
     4861 gctctcgccg cgggcgaacg caacgatcgg ctgcgcctcg caaccctcgc cgtagcagag
     4921 cgccaccggc tgcaccggtg catccgcctc gaccgcggcg gcgaagatgc gcgcgtggaa
     4981 ggcacccagt tcgcggccgt cgcgggtacc gccttccggg aacgcggcca ccgcgcggcc
     5041 gctgcgcagg cgcgcgctca tctcggccat cacgtcaccg agcgaattgg cgttgccgcg
     5101 ctgcaggtag atggtctccg catgcgaggt gatccagccg accaccggcc agcctcggat
     5161 ctcggacttg gcgatgaacc ccatcatgtg ctgcgaatgc agggcgacga tgtcgaccca
     5221 gctgatgtgg ttggccacga acagggtccc cccgggcagc ggcgtgccca tcccctgcac
     5281 gcgcatcccg aacacccgga gcagcatgcg cgcccacagg cggatcgccg cgtggccgat
     5341 cgggcggcgg ccgtcgtcgc cgatcgcgat cagcaccagc agcaccggca ggtgcacgaa
     5401 cacatgccag agcaggaatg gcaggcgata ggcgtagcgc agggcgcgca ggaaaaactc
     5461 gacggcgggg gacgtcgcgg gcatgggccg gaaccttagc gggcaaaggc tggagcagtc
     5521 agcctagaca ggcctcacgc ctggccgctg gcgggcggct tcggcaacac ggccaaggtg
     5581 atccggctga tgcagaccgg cttgccggcc gcgtcttcga tgcggatttc ccacacctgg
     5641 gtgctgcggc cgacatgcag cggccgcgcg gtgccggtga cgaggccgct gcgcaccgca
     5701 cgcaggtggt tcgcgttgat ctccaacccg accaccgcgt cgccgcgggc ggccagcatg
     5761 ccggcgctgc tgcccagggt ctcggccagc gccaccgagg cgccgccgtg caacagcccg
     5821 tacggctgat gggtgcgggc atccaccggc atggtcccgc gcaggaaccc gtcacccagc
     5881 tcggtgaagc ggatcccgag cgcctccatc agggtgccgg tggaacgcgc gttcagcgcg
     5941 tcgaggctgg catcctctgg ccagctgaat gccgagatgg ccaagcgcgg ctcagccgaa
     6001 cttgaaacca ccggggcgcc agctgcgcgt atacgacttg ctgccgcccg gcgcaccgta
     6061 accgccggag ccgccgtagc cgctgctgcg gccgtagccg ccgccgatgt cgcgttcgcg
     6121 gcgctggtgc atgcgctcga tcagttcgtc gcgacggccg tccagccagt cggtgcggcc
     6181 cagcgtggcc ggctccacgc cacggcgact ggcttcgttc gcgcggtagt cgcagaagtc
     6241 gtcgtaggcg tccagctcgt gcttcagttc gcgcatcgac agttcgatca tcaccgcgtc
     6301 gtcgaggaag ccgagcacct cgacatggtc cgggatcacg tcattcggat cgcagaagta
     6361 ggtcagcgcg gacagcacgc gctgcttgtc gtcgtcgggc agcgcccagc cagtgtcgcg
     6421 caccatcgcg atcatgtcgt ccaggcgcag caggcgctcc ttgatgaagt ccgggatgtg
     6481 caccttctgc gcatcggcca gcaggcccac cgcgcaatcc acgatctcct gctcggactt
     6541 gccctccgcc gccttgcggg agcgttccgc cgcttcctgg aaatgggcga ggtcgcggtc
     6601 attgagctcg aagttcaggg tgatcgacat ggcggcatcc ggcgcgcgta aagagaccgg
     6661 cagactaggg cccgcgccgc agcgcgtcaa tcgcaggcgc ccccgtcaag aggccggtct
     6721 tacgcccgcg aacgcgcgcc acgctcacaa gatcggcgac aagcccgagc cgaacgcggt
     6781 gaacacgcgc acccccagcc atttcaggcc gatgtccact tccggcgagt tgccgaccgg
     6841 ggtgtggcag gcgcggaagc gggggtcgaa cggcgacggt ggcgtggggc actccgggcc
     6901 ggggacgaat tcgtagctgg tcgcgtactt gatcggccac aggtcgaaga tcggcaggcg
     6961 ctcggaaatc cgcgccaggg tgtactcgac ccccgggagg atcggcgacg aatcccggcg
     7021 gccgatcgtc cacgcgttgc ccggcgccat gtcgcgctgg atgcttgccg ccagtcgcgc
     7081 ggcgaaggcg ggatcctcga tcaccaccgc gctctcggtg ttgtaggtgt caccgcgcgg
     7141 atcgaagttg tgggtaccga ccacgccgac acggccgtcc accaccatcg acttggcatg
     7201 caggcccatg cgcatgccgg cctgcttcaa ccgcaccggc atgccgcccg atccgatcga
     7261 gcggaagcgc ccggcctcgc tgtccggatc cttgcgcagg ccgcgcttcg ccagcaggcg
     7321 ctcgcgcagg gttggcctcg ccccggccga cgccggcaag gccggtgatg ccgcggtgtc
     7381 cgtgaagccg gggaggtccg cgccaagtgc ccccggttcg aacggggcat ccgcggggaa
     7441 cggcttgaac tcgtggatct cgaagccgaa gtcgcgcagg tagcggcgct tgtacttgtg
     7501 cgagatcgca taggcgatga aggcgtcggt cgaggccagg ctgttcgtgg agatgcgcac
     7561 gcgcggcgga tcgtcctgcc ggcgcaactc gcggaacagg tgctgggcag gcttggacag
     7621 caccaggtag ggcgtctgca gcagcacctc gtcgtgggcc ccggcgatca gcgtgcgcaa
     7681 gccgctgctg gcggcctcgg cagcggcgtc ggcctccgcg gggcgaccct gccagtgctt
     7741 gaccggcagg tcggcgatga actgcacgcc ctgcaccggc attgcccggt cgacgaaacg
     7801 cgcgcgaatc gcagcgtcgt catcgaccat cgccagtagc gcgcgcacgc gctccggctt
     7861 gcggtacggc gcatgcggtg gcggcggcgc gccctgcttc tgcagcaggc gggcgacgtc
     7921 gcccagctgc tcggccggca cgctgcgcgg ggacgaccag aacgcctcga aattcgtggc
     7981 catctcgccg gccaccgggc ctgcgaccag caagtcgcgg tcgcggaagt tgtagccggc
     8041 cccccagtcg taatagtcgt cctggtagtt gcgcccgccg gtgataccga cctcgccgtc
     8101 gaccagcaac agcttgttgt gcatgcggct gttgagctgg cgccagcagc aggccgcagc
     8161 caccgcgtac atcggcagcg ccaggcgcgc gcggtcgagc accgggttgt agaggcgcaa
     8221 ctcgaaattg gcgtgcatcg acgccagccc ggccagggtg tccacctggc gcagcgccgc
     8281 cagctggtcg accagcaccc gcaccttcac gccccggaac gcggcggcct gcagttcgtc
     8341 cagcaccagt tgggcggagt cgtcctcgtc gaagatatag gtctgcaggt cgatgctgcg
     8401 ttgcgcgctg cggatcaggt ggatgcgcac cagcagcgca tccggcgcgt cgtccaggat
     8461 cagggcgaca tggcgcgggg cggccggggt ggacgcggcg agcgcctgcc ggccgagcgc
     8521 gcgcagcggc gacgccagcg cgcaggcatc cgcccgcgtg caatcgacct ggctgctgcg
     8581 ggcggcgcgg gcgatgccgg cggcttcgtc gcgctgctcg acggagaggg tggcgcagcc
     8641 ggccagcaac agcgcacacg cgcccgccag cacccgcctg aaaaggggcc agagtgaagt
     8701 tttgcggcga gccttgcggg tgttgccgat gtcgggcagc gcagaattca ctctggcccc
     8761 ttttcaggtc atggccacca gaactgggtc agggtggcga tgccgggttc gatgttcacg
     8821 tccatcggca gccgcaacac cgcgatcccc cccggcggca tgccgcggta gtcgccggtc
     8881 tggccgctgt gcaccagcgc cgccagttgc tccaggccgg ggttgtggcc caccagcagc
     8941 aagcgttcgg cgtcgcggtt ggcatcggcc agtgccacca gggtgcccgg gctggcgtcg
     9001 tagatggacg cgtcctcatg cacttcggca ccgccgatgt cgcccagcgc agccagggtg
     9061 tcgcgggtgc gcgtggcggt ggagcacagc acgcggtcgg gtcgcagccc gtgttcgcgc
     9121 agccagtcgc cggcggcgcg cgcctccgcc aggccggtcg cggacagcgg gcgggcgatg
     9181 tcgtcctggc ccgcggtggc ggattcggca tgggcatggc ggagcaggat cagttcacgc
     9241 atggacactc cttggtcgcc ctcgcgggct cagtctttct tcagccagtg cagcagcggt
     9301 tgccagtcgg cctggtggtc gcggacctgc gccgagtggt agtcgaacag gctcttgccc
     9361 agcccggtca gcaccacgta ggcctggctg tcgcgcagtt gcgcgaccag cgggaacggc
     9421 cagctgcgca acacgtccat cgcctgctgc gaggcattgg tattcggttt gaggcggttg
     9481 cccaccagtc ccacccgcaa cttgcccttg cgcacgcgcg ggtgccggcc cagcgattcc
     9541 aggaagcgca cgctggcctc gatgtcgagc gccgagggct gcactggcac gatcaccgca
     9601 tcggcggtct ccagcacctc gtccaggtcg tcggccatcg cgccggccgg cgcgtcgacg
     9661 atcagccgct gggcatcgtc cgggacgcgc ttgcgccagg ccttgcgggt gccgtcgagc
     9721 ggcagcaccg cgctggccag gccggcgcgg cgctcgcacc agcgggtcgc cgagccctgc
     9781 gggtcggcat ccaccagcac cgtgcgcagg cccgacaacg cggcattcgc cgccagctgg
     9841 gtggcgatcg tggtcttgcc gacgccaccc ttcgaacagg ccaccagcca tgtcttcatc
     9901 gcgctgctcc gttgcgggac ggccccagcg tacacgcggc ggatggcccc tgcatgcgat
     9961 gtgttcaggc cggatagcgc tgcgccacgt ccagccgcag gcgcgcggcg gcggtatgca
    10021 cgtccaccgc cagcggcagc acgctggccc agtcgccacc gcggccggcg ccttccagcg
    10081 ccgccgcggc ctcggcaagc tccagcgcac cgaccagccg cgccgcgccc ttcaccttgt
    10141 gcgcctggcg ggtcaggccg tgcaggtcac cggcgccgcg cagttgctcc agcaccgcca
    10201 ggtcttcgtc ggtactgccc aggaaatcct ccagcagcgc gcgggcctcg ccgacgttgc
    10261 cgccgatgag atcctccagc accgacacct gcagggtcgg cggatcttcc agctgcggca
    10321 gcccggcctc ggtcgcgact gcctgccggt tgttggcgct ctggatacgc gcgccctgtt
    10381 cggcgccgac catcccgctg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnggttgttg gcgctctgga tacgcgcgcc ctgttcggcg ccgaccatcc cgctgccggc
    10561 ggtatgcggc agccagcgct gcaggcaggc acccagggtg gcaatgccga ccggcttggc
    10621 caggtagtcg tccatccccg cggccaggca ccgctcggcc tcgcctttca gcgccgaggc
    10681 ggtgagcgcc acgatcgggg tcggcggcaa cccgcgccgg gccgcttcct cgcggatcgc
    10741 gcgggccagc tggtaaccgt ccatccgcgg catgtgcacg tcgctcagca gcagcgcgta
    10801 acgaccgctg cgccagcgct ccaggccttc gacgccgtcc tccgcggatt ccgacgcata
    10861 gccggccagc gccagctggc gctggatcac ctggcggttg gtggcatggt cgtcgaccag
    10921 cagcaccagg ctgcgctcgg cttcggcctc gctgaccgtg ggcaggcggc gcggggtgaa
    10981 gccggccacc ggcttgcccg gcaacagcag gtcgggcaat tcgtccggcg gtgcgcgttg
    11041 cagggtgagc gccaggcgca tggtggtgcc cgccccgggg gtggattcca tgtccacccg
    11101 gccgcccatc agttcgccga tgcgccggct gatcgccagg cccaggccgg taccgccgaa
    11161 acgccgggtg gtatcgactt ccgcctgcga gaacggctgg aacagccgcg cctgcgcctg
    11221 ctcgctgatg ccgatgccgg tgtcggtcac ccggatcacc agtgcgtcgc tgcccagcgc
    11281 gccgtcggcg ggatcatgcc ggcccggctc caccgccacg gtgacgccgc cgtgttcggt
    11341 gaacttgatc gcgttggaca cgaagttgcc gaccacctgg cggatccgca gcggatccgc
    11401 gtagtgcgcc ggcgcgatcc gcgggtcgac ctggcaggac agcgccaggc ccttgctcga
    11461 cgccgacccg gcatagttgg ccaccacgct gcgcaacagg tccggcagcg aggtcggcac
    11521 cggctcgatc tccatccgcc cggcctcgat cttcgagaag tcgaggatgt cgccgatgat
    11581 ccgcagcaac gcctcggccg aggcctggat cacgttgagc gagcgccgct gctcgatgtc
    11641 gaggtcggtg tgcgaaagca cctcgatcat cccggtgacg ccgatcatcg gcgtgcggat
    11701 ctcgtggctc atcgccgcca ggaaggcgct cttggcgtgg ttggcgtcgc gcgcgcgggt
    11761 ctcgctgtcg cgcagcgcgc gttccagccg cttcagcacg gtcaggtcgg tcgccaccgc
    11821 cagcaggccg accatcgcgc cggtgtcgtc gcgcatcgcc gacagcgcca gcagcaccgg
    11881 cagggtgcgc ccgcgctggt ggcgcagcac gaattcgcgc ggcggctcgc gatgctgggc
    11941 gagcgcgcgc aacgcggtcc agtctggcgc gatgcgctgc ccgtaggcct cgctcagcga
    12001 gcgcgccagc gactgcaagg cctccgggtc gaggatcgcg tccatcgact cgcgcccgag
    12061 cagcgagccc ggcggccagc ccagcaactg ctcgccgaag ggattcacct gcgagaacac
    12121 gccctcctcg tcgaccgcga agatcgcggt ctgcgccgcc tccagcagca gccgctggaa
    12181 atgctcgcgg cgctccagcg cctcgcgcac gcggtcgttg ctggccagct ggtcggtcag
    12241 ctgggtctcg atgcggcgca cgtggttgcg catgcgcagg aaactgtcca tcacctcgcc
    12301 cagttcgccg ctgggcgtgt agtggggctc cgcggtgtag tcgtgctggg ccatccgtcg
    12361 tgccagcccg tgcaggcttt ccacgccgcg atagccgttg cgcaggatcc gccggccgaa
    12421 cagcgccacc agcaggaagc acagcagcga caacacaatg cggatgcgcc cgacccactt
    12481 gctgcgggtg agttccgcct gcaccagcgc ctcggcgcgc agttgcccgt gctcgctgat
    12541 gcggtcgagg cgttccacca gcggatcgac cgcgggatac agctcgcggt ccgcgaactg
    12601 gccgagcgcg cggatgtctt ttgcctgcag gatgcgctgc agggtttctg cggcctggtc
    12661 ggcgcgcacc cgcgcctgca aggcctgatc cagcaacgcc ttgtcctcgc cttccagcgg
    12721 cagcgcctgc aacgcgtgcc attcctgcgc ggcactgttc ctggccgccg tcaccaccgc
    12781 cacgccttcg tcccagtcga gcaggtggtt gcgggtgcgg aaggtggtgt ccaccacgtc
    12841 ctgcgcgtag gcatcggtga ggcggcggat gcggcccatg cccgccagca cgtcgtcctt
    12901 catcgcagcc agcgagcgct gggtgtagtg ctggccgatc tcgtcgatcg aaaggaccag
    12961 cgcgcaggtg atcaggaaca ggccgaacag gcccagcaac tggtggcgaa ggccgaagcg
    13021 cgacaggtca aaccgcatgc gggagatcca cgacggcggg cggaggcctg ccgcacatgc
    13081 tcagtgcgcg ggccggcgcc agcgcgcgat cgcggcctgc agttcgctgc ccggcaccgg
    13141 gcgcccgatc aggtagccct gggcgatgtc gcagccgagt tcggccagca tctgccagtc
    13201 ctccatgtcc tccacgccct cggccaccgc ggtcaggccg agcttgcggg ccaggtccag
    13261 gctggcttcg accaccgcgc gcttgcgcgg ctgcgcatgc gcgccgaaca cgaagccctg
    13321 gtcgatcttg agttcggtga acggaatctg cgagagctgc gccagcgaag aatagccggt
    13381 gccgaaatcg tcgatcgaca ggccgaagcc cttcaggcgc agacgcgcca gcaggcccag
    13441 cccgcgcgcg gcatccgaca tcaccgagct ctcggtgatc tccaggatca cgtcttccgg
    13501 ctccacgccg tagctctcga cgatctcctg gtagcgatcg gccgccgacg gatccgccag
    13561 cgaggccgcg gagacgttca ccgacagctt cagccggtgg ccgtgctggt cccagccctg
    13621 tttccagcgg cacgcggcat ccagcatgcg gtcggtcagc ttgttggcca ggccttcgcg
    13681 ctccagcatc ggcacgaaca ggttcggccg caccaggctg ccgtcggggc gctcccagcg
    13741 cgccagcgct tcgacgccga tcaccttgcc gttgccgaat tccacctgcg gctggaacca
    13801 cggcacgatc tcgccgcgct ccagcgcgtc ggccagtgcc tgcaggctga agtcggcctc
    13861 gccgtcgtcc tgttcttcgc cggcgttgtc ttcgaaacgg ttgagcgtgg cttccagctt
    13921 gtcgcgggtc agcggcttct ccacgctgcc cagcacgcgc aggccatagg cacgggccat
    13981 ggtctgcacg gtattgagca acgccgggtc gagcgcactg accaccacca ccgcgcgcgc
    14041 cagccgttcc tgggcgatgt ggccgatgca ctcgatgccg tccatgcccg gcatgtccag
    14101 gtccaccagc accacgtccg gcggcgtcgc ctgcttgcgc agcacctcga gcgcatgcag
    14161 gccatcggca ccctcgagca cgcgctcgat gccgagctcg gacagcaggc gcagggcgat
    14221 ccggcgctgg aagccgtgat cctccaccac catgacctgc agttccgatc cgctcatgcc
    14281 atctccccga tgcaggcgtc gaacgccaag cctaccgcag ccgctggcgg cttgcgcgcg
    14341 cagccgccgt gccgcccgcg aatgacgcgc gggtcagggc gtggccggca ccagggtcgc
    14401 gcggtcgagt acccacatcg ccgggcgagt atcgccggtg aaccacaggc acaggtcggt
    14461 cgatgccggc gcatcgggca acggcgcgcg cagggtcacg aaaccgtcgg catcggtggt
    14521 ttccggcagc ggcacggtgg ccagcaacgg gccgtcgcag ccggcatgca gttgcagttc
    14581 gccatgcgcg gtctttgccg gcttgaacgt gcgcgccggc tcgtcgtggg ccagctggaa
    14641 gaaatacggg atgcgcccgg cccgcacctc gaccgcgccg atcccggcca gcggcgcctg
    14701 cttccacgac cagcacggat tgaagatgtc gacgttgtag atcgcccgcg cgccgtcgcg
    14761 cgggccgtcg tcctccagcc gcagcatcag ctggccggtg cacatcgcca gttcctcgtc
    14821 gctgcgactc agcagcgatg cggcactgag ttcgcgcgcg accggtggtg ccaaggccac
    14881 gccatcgaag aacgcacccg cctgcagcgt cgccggcaac gccagcgaga gcggctgcgc
    14941 atacagcggc gagcgcgcgg tcggcgtgct gccatcggtg gtgtagcgga ttgcatagcc
    15001 cagcggctgc gacagcgtga ccgtggcctt gccccgcgca tccgcctgcg cgacgtcgac
    15061 cagcggctgg aacggcgtct tcgcataggc gatgccgcgc gcgtcccagc gcggcagcag
    15121 cagcggcagc cgctgcagga agccggcata gtccttgccc tcgcgcgacg accacgcact
    15181 ttccgccagc gcggcgatgc gcgggaacac cgcgtgctgc acgcgggcga agctgcgcat
    15241 gtgctcggtc cacacgttcg cctgcacgcc aaggatgtgt ttcgcctggg tcgcatccag
    15301 caccgccggc accggctcga acgcatacac cgtttccagc gtgacctggg tgggacgccc
    15361 cggttcctcg gtcggcgaca tcgtctgcag gtagtccagg tacagcttgt cccacggcgc
    15421 catcaccacg tcgtggccac gcttcgccgc ggcgatgccg ccctcggtgc cgcgccacga
    15481 catcaccgcc gccgattccg gcaagtcgcc gtccaggatt tcgtcccagc cgatcaggcg
    15541 gcggtcgttg gcggacaggt aacgttccag ccgcgcgacc atccacgcct gcaggccggc
    15601 ctcgtccttg accccgagct cgcggatcct cgcctgcatc ttcggcgagg ccttccactg
    15661 gtccttgacg gcctcgtcgc cgccgatatg cacatagcgg ccggggaaca ggttcaccag
    15721 ctggcccatc acgtcctcga ggaaccgcat ggtggcgtca tcagcattga acaggttgac
    15781 gttgacgccc cattcgttgg acaccgcgat cggatttcct gacacgccga gctgcggata
    15841 cgcggagatc gccgcggtgg cgtgaccggg aatgtcgacc tcgggcacca cgtcgatgtg
    15901 cagggatgcc gcgtgcgcga ccacttcggg gatct
//
LOCUS       DAOOCX010000047        15932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3306_length_15932_cov_8.115387, whole genome shotgun sequence.
ACCESSION   DAOOCX010000047 DAOOCX010000000
VERSION     DAOOCX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15932
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3306_length_15932_cov_8.115387"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /gene="serA"
                     /locus_tag="PK399_06205"
     CDS             <1..899
                     /gene="serA"
                     /locus_tag="PK399_06205"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005915394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPW12743.1"
                     /translation="ELVLAEAIMLLRGIPQKSAQCHRGGWSKSAAGSREARGKTLGIV
                     GYGHIGTQVGVLAEGLGMHVIFHDIETKLSLGNARASRDLDELLAASDVVTLHVPETP
                     ATQWMIGAAQLAKMKPGAHLINAARGTVVDIDALAAALQSGHIGGAAVDVFPEEPKGN
                     DDPLVSPLLGIDNVILTPHVGGSTLEAQDNIGIEVAAKLVRYSDNGSTLSAVNFPEVT
                     LPEHAGSHRLLHIHRNVPGVLSRINELFGQRGINIDGQFLRTDANVGYVVIDVAADDA
                     QASELRAALSAIPGTLRTRVLY"
     gene            complement(980..1528)
                     /locus_tag="PK399_06210"
     CDS             complement(980..1528)
                     /locus_tag="PK399_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPW12744.1"
                     /translation="MAEFDDLVLASALAEYAARWPEEGTTVDVFKELLGDAANPFVRD
                     RLAGHFTASCWLVDRSGARTLLTHHRKLGLWLQLGGHADGERDLRIAALREAEEESGL
                     RGLRIEPAIFDLDRHWIPEHKGVAAHWHYDVRYVVHAGEDEAYTVSDESHDLAWRRIA
                     ELAAEAGTDPSVQRMARKWMAR"
     gene            1681..3261
                     /locus_tag="PK399_06215"
     CDS             1681..3261
                     /locus_tag="PK399_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPW12745.1"
                     /translation="MLFQRIKPLDKILETAQKNALVRQLGALQLTLLGVGAIIGTGIF
                     VLTAAAAQKAGPGMMWSFVIAGLVCAVAALCYSEIASMVPVSGSAYTYTYAVMGEILA
                     WMVGWALILEYAVAASAVSVGWSGYFMGLVKSATGFELPALLSAGPAWSFAGGIPSVD
                     FSHGVFNLPAVVIALAVTWLLMIGTTESARVNAILVAIKILALSAFVALTVPVINGAH
                     FEPLAPNGWFGNDGGSGLGIVGAAASIFFAYVGFDAVSTAAEETKNPQRNVPIGLIGS
                     LALCTVFYLLVAAGAVGAIGAQPTALGVQPGSPEFVAQCQALLAQGQEPLVCSNEALA
                     HVLRVIGHERAGDMVGLAANLALPSVILMMLYGQTRIFFVMARDGLLPQSLAQVHPKW
                     KTPYKVTLITGIFVAIAAALFPVGQLADISNSGTLFAFFMVAIAVLILRVKDPTRHRP
                     FRTPFVWVVAPLAIFGTLFLFFNLPFEAKIVLPIWGGIGLLFYFMYGYRKSHLARGNL
                     EVHETDSDIPPPPVPPIQ"
     gene            3361..3993
                     /locus_tag="PK399_06220"
     CDS             3361..3993
                     /locus_tag="PK399_06220"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HPW12746.1"
                     /translation="MQTLPYDAQHLRHLAGEIITNVRELAHAGWTPATSSNFSRRLDD
                     RHAAITVSGRDKGRLTEADIMVVDFDGNPVGNDKRPSAETLLHTQLYRRFPEIGCVLH
                     THSRTQTVASRLYAPAGRVRFEGYELQKAFFGNTTHEGAMDVPVFPNTQDMHQLAGWV
                     ETALDAQPMWGYLIDGHGLYAWGRDMAEARRHLEAFEFLLGCELELRKLQ"
     gene            3990..4547
                     /locus_tag="PK399_06225"
     CDS             3990..4547
                     /locus_tag="PK399_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone dioxygenase"
                     /protein_id="HPW12747.1"
                     /translation="MSRLRIFDDHAPGAPLLATSDRAGMGAELAKIGVHLEQWEPAHA
                     VQPGDAPEAIMAAYRADIDRLVEAHGFRSVDVVSIAPDNPNREAMRGKFLDEHFHKED
                     EVRFFVAGSGLFTLHVGGKVYEVLCEAGDLIAVPDSMTHWFDMGPEPSFVAIRFFTEP
                     DGWVGHFTGTDIAQKFPRYEIGQAI"
     gene            4597..5292
                     /gene="mtnC"
                     /locus_tag="PK399_06230"
     CDS             4597..5292
                     /gene="mtnC"
                     /locus_tag="PK399_06230"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HPW12748.1"
                     /translation="MKTTILTDIEGTTSSISFVRDVLFPYARDALPDFVREHGHDPRV
                     RHWLDVVAVENGGLCQDAMIAETLQGWIDQDRKHTALKALQGLIWEAGYREGDFTAPL
                     YPDVAPALRAWHAAGHGLAVYSSGSVPAQKLLFSHTDAGDLVPLFRDFFDTEIGHKRD
                     ADSYRLIADRLDRSPGDILFLSDVVAELDAAREAGMRTVLLDRIEDYPQPRITDDATH
                     GHARAEAFSQVTP"
     gene            complement(5296..6648)
                     /locus_tag="PK399_06235"
     CDS             complement(5296..6648)
                     /locus_tag="PK399_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HPW12749.1"
                     /translation="MHSLLLATMLALGAPVEVSTPACVPVQVEAPLPLPRSDRSGPGC
                     IAYQRTTRAGHGDALRVLVFGDPQVKSARDVDYFRRDIVAPLIGTHDASLGITLGDLV
                     DDQPALLPSVKAVTAELGVPWLHAPGNHDVDPDATSDADSLGAFHRAIGPDTFARQTA
                     LANLAVLDDVIAMPGRKPAYIGGLREDQFAFLERWLPTLAKDRLLVLAMHIHLFDEAG
                     KDSFRDADRERLFALLKPFPHVLVLSAHSHTQRHVFHDAATGWHGANPLHEYNVGTAC
                     GAYWSGAADRQGIPAATMPDGTPNGYAVLTVKQGGDYALAWHNARDPDDRQVGLHAPK
                     ALRRGAYPAWGVFANVYMGMDDTRVEFRVDGGEWMPMKKVLQPDPALLAENALDDASD
                     VLRGFDRSPEAEASTHLWRGALPTKLAAGEHVVEVRAFDRWRGETRAQTTYRLIDAGP
                     "
     gene            6750..8555
                     /locus_tag="PK399_06240"
     CDS             6750..8555
                     /locus_tag="PK399_06240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW12750.1"
                     /translation="MQAWLLAAVMILLASAWPAGAVEPLRLAGERAIPLNQVIAYRHD
                     VHASDGATEAFAHARAGRFSPLPGGSPNFGFQAGAYWFYLPLVNTQRDEPRWLLVQQY
                     ALSDQLDLYLRYPDGRVEHHASGDHVPFNSRYIRYRHPNFRLDLPHGQQVELLVRVQS
                     QSSMQVPLVLYTPRAFAEVMRDAQFSTGLYYGIVLALLFYNLVLWLMLRDSGYFWYLL
                     HTGAFGLVLFTLNGYGFEYFWPNSAWLADASVPLSICLALVGMQLFTRSFLELQERWP
                     RGNLVSLMVTGFFVVLGIASFWLPYSTATPLASRAVLAGVVWIMVATVVMLRRGYKPA
                     RLLLLAWSLFLAGTTAFTLLAFGLVPPNFWTLNGVQIGSALEMLLLSLALGSRYAGLR
                     IENIRIVQENNERLERSVIDRTRQLRTAMAQLGEANVQLREYSQRDPLTGVYNRRHFR
                     EALSQALELRQASDQPVALLLLDLDHFKKINDTHGHLVGDDCLNVAARCIEDVVVPRG
                     GIVARFGGEEFVAVLPGLDAQQALQLAEAVRLRIHQTPVDSDGRSIHLSASIGVHTAT
                     GERATPEDVIRLADEALYRAKDDGRNCVRHSVSLV"
     gene            complement(8703..9320)
                     /gene="hisIE"
                     /locus_tag="PK399_06245"
     CDS             complement(8703..9320)
                     /gene="hisIE"
                     /locus_tag="PK399_06245"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HPW12751.1"
                     /translation="MTRTIDFDFDIEALAWDKQGGLLPAIVQEADTLRVLMLGYMGRE
                     ALQATLDSGKVTFFSRSKQRLWMKGETSGDVLDLVHIETDCDADTLLVQARPHGPTCH
                     LQRASCFPNAPADTLAALDGLVATRERDRPAGSYTTRLFEGGVRRIAQKVGEEGVETA
                     LAGVVQDDDALLGEAADLLFHLIVLLRARGLSLADAKRVLESRRG"
     gene            complement(9317..10087)
                     /gene="hisF"
                     /locus_tag="PK399_06250"
     CDS             complement(9317..10087)
                     /gene="hisF"
                     /locus_tag="PK399_06250"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPW12752.1"
                     /translation="MLSRRIIPCLDVREGRVVKGVKFRDHVDMGDIVELAMRYRDQGA
                     DELVFYDITASPQGRSVDRGWVERVARVIDIPFCVAGGIRRVDDARAILLAGADKVSV
                     NTPALERPALISELAEAFGVQCVVVGIDSVLDADGEWRVRSHTGDPDAMRAPGKRTLD
                     WVVEAQALGAGELVLNCMGSDGVRGGYDIDQLRAVRARCGVPLVASGGAGEPAHFTEV
                     FRQADVDAALAASIFHSGAVAIPALKQALRVQGIEVRL"
     gene            complement(10081..10815)
                     /gene="hisA"
                     /locus_tag="PK399_06255"
     CDS             complement(10081..10815)
                     /gene="hisA"
                     /locus_tag="PK399_06255"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPW12753.1"
                     /translation="MSFIVYPAIDVREGRVVRLRQGDFAEETRYGEDPLAFAQRYADA
                     GAAWLHLVDLDAAKAGGYTLQPLLRGIAATTSLQVQTGGGVRTRDDVAAILDAGASRV
                     VIGSLAVRDSASVLAWLGEFGSERITVALDTRRDADGIWRLPVHGWTETAAETLDDLL
                     RRYADGGLRHLLCTDIDRDGMLTGPNIALYREIRAAHPGLQLQASGGVRDVADVVAAR
                     GAGCAGAVLGKALLEGRLSLPGALQC"
     gene            complement(10812..11414)
                     /gene="hisH"
                     /locus_tag="PK399_06260"
     CDS             complement(10812..11414)
                     /gene="hisH"
                     /locus_tag="PK399_06260"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPW12754.1"
                     /translation="MTTLALVDAGGANLGSVRYALERLGVEARIVRDGDGLCDAGRII
                     LPGVGAAAPAMALLRERGLVEPLRAATVPLLGICLGMQLLFESSDEGDVACLGLLPGR
                     VRKLRGGPGLRVPHMGWNGLEHVRDSALLDGVADGASTYFVHGYAAPVTADCVAACTH
                     GERFAAMVQRGNVAGAQFHPERSATVGAQLLSNFLQWNGA"
     gene            complement(11411..>11880)
                     /locus_tag="PK399_06265"
     CDS             complement(11411..>11880)
                     /locus_tag="PK399_06265"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008571443.1"
                     /note="catalyzes the formation of
                     3-(imidazol-4-yl)-2-oxopropyl phosphate from
                     D-ethythro-1-(imidazol-4-yl)glycerol 3-phosphate and
                     histidinol from histidinol phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase"
                     /protein_id="HPW12755.1"
                     /translation="FDHMLEQIGKHGGFALDVRTDGDLHIDEHHTVEDTGLAIGQALK
                     EALGDKRGIGRYGFTLPMDETLASAALDFSGRPYFVFEGEFKRERVGDLPTELVPHFF
                     RSVCDASGLNLNLKVQGDNDHHKVEASFKALARALRQAIKREGAELPSTKGTL"
     assembly_gap    11881..11980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11981..12645)
                     /gene="hisB"
                     /locus_tag="PK399_06270"
     CDS             complement(<11981..12645)
                     /gene="hisB"
                     /locus_tag="PK399_06270"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPW12756.1"
                     /translation="MSGTPILFVDRDGTLIEEPEDFQIDAYPKLRFVRDVIPAMLKLR
                     DAGYEFVIVSNQDGLGTEAYPRESFQGPHDLMMQVFESQGIRFREVLIDESFPHDNKP
                     TRKPGLGMMVGYLQDRGIDWSRSGMVGDRPTDIEFADNLKIRGFQLKTAQFGGEWDWA
                     GIAHELADAPRRAVVQRNTKETKIRVEVDLDRVAEPRVHTGLPFFDHMLEQIGKHGGF
                     ALDV"
     gene            complement(12642..13733)
                     /gene="hisC"
                     /locus_tag="PK399_06275"
     CDS             complement(12642..13733)
                     /gene="hisC"
                     /locus_tag="PK399_06275"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPW12757.1"
                     /translation="MNATVNALLRADLRDFGGYKSARSDLVDGNVWLNANESAWANPS
                     DADATLRRYPDPQPRALRSRLAALYDVEPAQLLVGRGSDEAIDLLVRAFCAPGQGAIA
                     IAPPVFGMYAVCARLHGARVIDVPLVDGEQGLRVDLQALGDAALANGASLVFLCTPGN
                     PSGEALPLIEIASLAVRLRGKALVVVDEAYAEYSDVPSATALFPAHENIAVLRTLSKA
                     HALAAARVGCVLGAPALIEALRRCQAPYPMPQPVTEAALKAMAPQALASTAARVAETR
                     EWRERLAGGLRRLPQVLRVYPSSGNFLLVRFIDADAAFAQLLEAGVVVRDMRAMPQLG
                     DALRITVGMPDECRRVLAALDADAVEVAA"
     gene            complement(13730..15028)
                     /gene="hisD"
                     /locus_tag="PK399_06280"
     CDS             complement(13730..15028)
                     /gene="hisD"
                     /locus_tag="PK399_06280"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPW12758.1"
                     /translation="MKRIDWNALDEREREAVLRRPVQAVSDAVRDRVEAIFEDVAARG
                     DDALRDYTAKFDGVTLADFEVGTDEFAAARAAVPADVQSAIAEAASRIEAFHRAGVAA
                     PYELETAPGLLCQRVQRPIARVGLYVPAGSAPLPSTALMLAIPAQLAGCREIILCTPP
                     RKDGSADPAVLLAAQQAPGVRVFKLGGAQAIAAMALGTGSMPRCDKLFGPGNSWVDEA
                     KRQALQRPGGIAIDMPAGPSEVLVIADAGADPAFVASDLLSQAEHGPDSQVLLVSDND
                     ALLDAVAVEVELQLARLPRAAIATQALAASRLVRVGDIAQAIEVSDAYAPEHLILAVR
                     EPRDWLDRVQNAGSIFLGDWTPEALGDYCSGSNHVLPTGGAARAWSGLSVASFQKAIT
                     VQDASRAGIAAAGPCAATLAHAEGLQAHARAVELRLERAA"
     gene            complement(15025..15915)
                     /gene="hisG"
                     /locus_tag="PK399_06285"
     CDS             complement(15025..15915)
                     /gene="hisG"
                     /locus_tag="PK399_06285"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPW12759.1"
                     /translation="MRDRLRIAIQKSGRLSEPARALLASSGLSWRESRDKLFCYGESL
                     PVDLLLVRDDDIPGLIADGVCDLGIVGRNVLAEQAGERAEAGKPEVFREWRALGFGGC
                     KLSLAVPDDWDWQGPQQLAGKRIATTYPNQLRAWLRTQGVDAEAVVLNGSVEIAPRLG
                     QADAVCDLVSSGATLAANQLKPVLDIMRSEAVLAGPAQPFGDVRAELADLLLRRMDGV
                     LRIRDSKLLMFQAPRDAVDQLLQLLPDAEPPTVCAVDGADTLALQALCHGAMTWQRLE
                     DLKRAGARGLMVLPVERMLA"
BASE COUNT         2530 a         5515 c         5262 g         2525 t
ORIGIN      
        1 ccgagctggt cctggcggag gcgatcatgc tgttgcgcgg gatcccgcag aagtcggcgc
       61 agtgccatcg cggcggctgg agcaaatccg ccgcggggag ccgcgaggcg cgcggcaaga
      121 ccctgggcat cgtcggctac ggccacatcg gcacccaggt cggcgtgctc gccgaaggcc
      181 tgggcatgca tgtgatcttc cacgacatcg agaccaagct ctcgctgggc aacgcgcgcg
      241 ccagccgcga cctggacgag ctgctggcgg cgtccgacgt ggtcaccttg cacgtgccgg
      301 agacgcccgc gacgcagtgg atgatcggtg ccgcgcagtt ggcgaagatg aagcccggcg
      361 cgcacctgat caacgcggcg cgcggcaccg tggtcgacat cgacgcgctt gcggcggccc
      421 tgcaatccgg ccatatcggc ggcgcagcgg tcgatgtgtt cccggaggaa ccgaagggca
      481 acgacgatcc gctggtctcg ccgctgctcg gcatcgacaa cgtgatcctg accccgcacg
      541 tcggcggaag cacgctggaa gcgcaggaca acatcggcat cgaggtggcg gccaagctgg
      601 tccgctacag cgacaacggc agcaccctga gtgcggtgaa cttcccggaa gtcaccctgc
      661 ccgaacatgc cggcagccat cgcctgctgc acatccaccg caacgtgccc ggcgtgctgt
      721 cgcggatcaa cgaactgttc ggccagcgcg gcatcaacat cgacggccag ttcctgcgta
      781 ccgacgccaa cgtgggttac gtggtgatcg atgtggccgc cgacgacgcg caggccagcg
      841 agttgcgcgc ggcgctgtcg gcgatcccgg ggaccttgcg gacgcgcgtg ctgtattgat
      901 ccgcttgctg ttgaagttgc agtccatcgt gggagcggct gtcgtttagc cacgccgggc
      961 ttccgtggga ggggcttcgt cagcgcgcca tccacttgcg cgccatccgc tgcacggacg
     1021 ggtcggtgcc ggcttcggcc gccagctctg cgatccgacg ccaggccagg tcgtgggact
     1081 cgtcgctgac ggtgtaggcc tcgtcctcgc cggcgtggac gacgtagcgc acgtcgtagt
     1141 gccagtgcgc ggcaacgccc ttgtgctcgg ggatccagtg gcggtccagg tcgaagatcg
     1201 cgggctcgat gcgcaggccg cgcaggccgg actcctcttc cgcctccctc agggcggcga
     1261 tgcgcaggtc acgctcgcca tcggcatggc cgccgagttg cagccacagc cccagcttgc
     1321 ggtggtgggt cagcagggtg cgcgcgccgc tgcgatcgac cagccagcag gacgcggtga
     1381 agtgcccggc gaggcggtcg cgaacgaagg gattggcggc atcgcccagc aactccttga
     1441 agacatcgac ggttgtgcct tcctccggcc accttgccgc gtattcagcc agcgcggatg
     1501 caaggacaag atcatcgaat tcggccatgt tgcgtcgcag gagcccgtgg gagcgggcga
     1561 ttatcgccgc aggatgaccc gcggcgcttg tgcgcggcgc atgtgaccgt ttaaggtcgc
     1621 gggcttgcgg cctgccctcg gcaggttcgt ctcatgaact agaacccggg ggatcacctg
     1681 atgctgttcc agcgaatcaa gccgctcgac aagattcttg aaactgccca gaagaacgcg
     1741 cttgtccgcc aattgggggc gctgcaactg accttgctgg gggtcggcgc catcatcggc
     1801 accggcatct tcgtgctcac cgcggctgca gcgcagaaag ccggccccgg catgatgtgg
     1861 agcttcgtga tcgcgggctt ggtctgcgcg gtagcggcac tgtgctattc cgaaatcgct
     1921 tccatggtgc cggtatccgg ctccgcctac acatacacct atgctgtgat gggcgagatc
     1981 ttggcgtgga tggtgggctg ggcgctcatc cttgaatacg ccgtcgctgc gtccgcggtg
     2041 tcggtcggct ggtccggcta tttcatggga ctggtaaaaa gcgcaaccgg cttcgagttg
     2101 cctgcattgc tgtcagcagg gccagcctgg tcgttcgcag gtggcatccc cagcgtcgac
     2161 ttcagccatg gcgtcttcaa cctgccggcc gtggtgatcg cgctcgccgt gacctggttg
     2221 ctgatgatcg gcaccaccga aagcgcgcga gtcaatgcca tcctggtggc aatcaagatc
     2281 ctcgcgctgt cagccttcgt cgcactgact gttccggtga tcaacggggc gcactttgaa
     2341 ccgctcgccc ccaacggatg gttcggcaac gacgggggat ccggactcgg gatcgtgggc
     2401 gcggccgcgt cgatcttctt cgcgtacgtg ggctttgatg ccgtgtccac cgccgctgaa
     2461 gaaaccaaga atcctcagcg caacgtcccg atcggactga ttggcagtct cgcgctctgc
     2521 accgtcttct acctgttggt cgccgccggt gcggtcggtg cgatcggcgc gcaacccacg
     2581 gcgcttggcg tccagccggg ctcgccggaa ttcgtcgccc agtgccaggc gctgctggcc
     2641 caaggccagg aacctctggt ctgctccaat gaagcgcttg cgcatgtcct gcgcgtgatt
     2701 ggccatgagc gtgccggcga catggtcggc ctggccgcca acttggcgtt gccgtccgtg
     2761 atcctcatga tgctgtacgg tcagacccgc atcttcttcg tgatggcgcg tgatgggctg
     2821 ctgccacaga gcctggccca agtgcacccg aagtggaaga caccctacaa ggtcaccctg
     2881 atcaccggca tcttcgtcgc gatcgcggcg gccctgttcc cggtcggcca gctcgccgac
     2941 atctcgaact ccggcaccct cttcgcgttc ttcatggtcg cgatcgcggt tctcattctc
     3001 cgcgtcaagg acccgaccag gcatcgtccg ttccgcacgc ccttcgtctg ggtggtcgcg
     3061 cccttggcga tcttcggtac cctgttcctg ttcttcaacc tgccgttcga agcgaagatc
     3121 gtgctgccga tctggggcgg catcggcctc ctgttctact tcatgtacgg ctatcgcaag
     3181 agccacctcg cccgtggcaa cctggaagtg catgaaacgg acagcgacat cccgccgccg
     3241 ccggttccgc cgatccagta acgagccttc tcgacaactg acgaaaaccc ggctccggcc
     3301 gggtttttgc ttgcccgcga ttcgacgatg cgtggacacc gattcgctac acttccgcgc
     3361 atgcaaaccc ttccctacga cgcccagcac ctgcgccacc tggccggcga gatcatcacc
     3421 aacgtgcgtg agctcgcgca tgccggctgg acgcccgcga ccagcagcaa tttctcgcgt
     3481 cgcctggatg atcgccatgc cgcgatcacc gtttcaggcc gcgacaaggg acgcctgacc
     3541 gaagccgaca tcatggtcgt ggacttcgac ggcaatccgg tcggcaacga caagcgtccg
     3601 tcggcggaga ccctgctgca cacccagctg taccggcggt tcccggaaat cggctgcgtg
     3661 ctgcacacgc actcgcggac gcagacggtg gcatcgcgcc tgtacgcgcc cgccggccgc
     3721 gtccgcttcg agggctacga gttgcagaag gcgttcttcg gcaataccac ccacgaaggc
     3781 gcgatggacg tcccggtatt cccgaacacc caggacatgc accaactcgc gggctgggtc
     3841 gagaccgcgc tcgacgcgca gccgatgtgg ggctacctga tcgacggcca tggcctgtac
     3901 gcctggggcc gcgacatggc cgaggcacgc cgccacctgg aagccttcga attcctgctg
     3961 ggctgcgaac tggaactgag gaaactgcaa tgagccgcct gcgcatcttc gacgaccatg
     4021 cacccggcgc accgctgctg gccacgtccg accgcgctgg gatgggcgcc gaactggcga
     4081 agatcggcgt ccatttggag cagtgggaac cggcgcatgc cgtgcagccc ggcgacgcac
     4141 ccgaggcgat catggcggcc taccgggccg acatcgaccg cctggtcgaa gcccacggct
     4201 tcaggtcggt cgacgtggtc agcatcgccc cggacaaccc caatcgggag gcgatgcgcg
     4261 gcaagttcct cgacgagcac ttccacaagg aagacgaagt gcgtttcttc gtggcgggct
     4321 ccggcctgtt caccctgcac gtcggcggca aggtctacga ggtgctgtgc gaggccggcg
     4381 acctgatcgc ggtcccggac agcatgaccc actggttcga catgggcccg gagccgagct
     4441 tcgtcgcgat ccgcttcttc accgagccgg atggctgggt cggccacttc accggcaccg
     4501 acatcgcgca gaaattcccg cgctacgaaa tcgggcaggc gatttgagcc aacctcgccc
     4561 caaccccccg ccgcgaggcg ggccagggag agaatcatga agacgaccat cctcaccgac
     4621 atcgaaggca cgaccagttc gatttccttc gtccgcgacg tgctgttccc gtatgcacgc
     4681 gacgccctgc cggatttcgt gcgcgaacat ggccacgacc ccagggtgcg ccactggctg
     4741 gacgtcgtcg ccgtggaaaa cggcggcctg tgccaggacg cgatgatcgc cgagaccctg
     4801 cagggctgga tcgaccagga ccgcaagcac actgcgctga aggcgctgca gggcctgatc
     4861 tgggaagccg gctatcgcga gggcgacttc accgcgccgc tgtatcccga cgtggccccg
     4921 gccctgcgtg cctggcacgc ggccggtcac gggctggcgg tgtattccag cggttccgtg
     4981 ccggcgcaga agctgctgtt ctcgcatacc gacgccggcg acctggtgcc gctgttccgc
     5041 gacttcttcg ataccgagat cggccacaag cgcgatgccg acagttaccg gctgatcgcc
     5101 gaccggctgg accgcagccc gggcgacatc ctgttcctgt ccgatgtcgt cgccgaactc
     5161 gatgccgcgc gcgaggcggg aatgcgcacc gtgctgctgg atcgcatcga agactatccg
     5221 cagccgcgca tcacggacga cgcaacgcac gggcacgcgc gcgccgaagc cttcagccag
     5281 gtcacgccgt aagcgtcacg gcccggcgtc gatcaagcga tacgtggtct gcgcgcgggt
     5341 ttcaccgcgc cagcgatcga acgcgcggac ctcgaccacg tgttcgccgg ccgcgagttt
     5401 cgtcggcagc gcaccgcgcc agagatgggt cgatgcctcc gcttcgggcg aacgatcgaa
     5461 gccacgcagc acgtcgctgg cgtcgtccag cgcattctcg gcgagcagcg cgggatccgg
     5521 ctgcaacacc ttcttcatcg gcatccactc gccgccgtcc acgcggaatt cgacgcgggt
     5581 gtcgtccatg cccatgtaca cgttggcgaa cacgccccag gccggatagg ccccacgacg
     5641 cagggccttg ggcgcatgca ggccgacctg gcggtcgtcc gggtcgcgcg cgttgtgcca
     5701 ggccagtgcg tagtcgccgc cctgcttcac cgtcaagacc gcgtagccat tcggggtgcc
     5761 gtcgggcatc gtcgcggcgg gaatgccctg ccgatcggcg gcaccggacc agtaggcacc
     5821 gcaggcggtg ccgacgttgt attcgtgcaa gggattcgcg ccatgccagc cggtggcggc
     5881 gtcgtggaac acgtggcgct gggtgtggct gtgcgcgctc agcaccagca cgtgcgggaa
     5941 cggcttgagc agggcgaaca ggcgctcgcg gtcggcatcg cggaagctgt ccttgccggc
     6001 ctcgtcgaac aggtggatgt gcatcgccag caccagcagg cgatccttcg ccagcgtcgg
     6061 cagccagcgc tcgaggaagg cgaactggtc ctcgcgcagg ccaccgatgt aggccggctt
     6121 ccggcccggc atggcgatca cgtcgtccag caccgcgagg ttggccagcg ccgtctgccg
     6181 ggcgaaggtg tccggcccga tcgcgcgatg gaaagcaccc agcgaatcgg cgtcgctggt
     6241 ggcgtccgga tcgacgtcat ggttgccggg cgcatgcagc cacggcacgc cgagttcggc
     6301 ggtgaccgcc ttgacggacg gcagcagcgc tggctggtca tcgaccaggt cgcccagggt
     6361 gatgccgagc gatgcatcgt gggtgccgat gagcggtgcg acgatgtcgc ggcggaagta
     6421 gtcgacatcg cgcgcggact tcacctgcgg atcgccgaag accagcacgc gcagcgcatc
     6481 accatggccc gcgcgcgtcg tgcgttggta ggcgatgcaa cccgggccgc tgcggtccga
     6541 acgcggcaac ggcagcggcg cttcgacctg taccggcacg caggcaggag tggaaacctc
     6601 gacgggtgcg ccgagggcga gcatggtggc cagcaggagg gagtgcatgc cgcattatcg
     6661 cctgccgcaa ggatccggcg gctggactag aatcgccgca cggggaccga tcgaacgggg
     6721 gtcgtttgcg cgcaaccacg ggcagcagga tgcaggcatg gctgttggcg gcagtgatga
     6781 tcctgctggc ctcggcctgg ccggcgggtg ccgtggaacc gctgcggctg gccggagagc
     6841 gcgcgatccc gctcaaccag gtgatcgcct accggcatga cgtgcacgcc agcgatggtg
     6901 ccaccgaggc ctttgcccat gcccgtgccg ggcggttctc gccgctgccc ggcggcagcc
     6961 cgaacttcgg gttccaggcc ggtgcgtact ggttctacct gccgctggtg aacacgcagc
     7021 gggacgaacc gcgctggctg ctggtgcagc agtacgccct gagcgaccag ctcgacctgt
     7081 acctgcgcta ccccgacggg cgcgtcgagc accacgccag cggcgaccac gtgccgttca
     7141 acagtcgcta catccgctat cgccatccca acttccgcct ggacctgccg catggccagc
     7201 aggtggaact gctggtgcgc gtgcagagcc agagttcgat gcaggtgccg ctggtgctgt
     7261 acacgccacg cgccttcgcc gaggtcatgc gcgacgcgca gttctccacc ggcctgtatt
     7321 acgggatcgt gctcgccctg ctgttctaca acctggtgtt gtggctgatg ctgcgcgact
     7381 ccggctactt ctggtacctg ctgcacaccg gtgccttcgg actggtgctg ttcaccctga
     7441 acggctacgg cttcgaatat ttctggccga attcggcatg gcttgcggat gcctcggtgc
     7501 cgctgtcgat ctgcctggcg ctggtcggca tgcagctgtt cacccgcagc ttcctggaac
     7561 tgcaggaacg ctggccacgg ggcaacctgg tcagcctgat ggtcaccggc ttcttcgtgg
     7621 tgctgggcat cgcctcgttc tggctgccct acagcacggc gacgccgctg gcctcgcggg
     7681 ccgtgctggc gggcgtggtc tggatcatgg tcgcgacggt ggtgatgctg cgccgcgggt
     7741 acaagccggc gcgcctgctg ctgctggcgt ggtcgctgtt cctggccggc accaccgcgt
     7801 tcaccctgct tgccttcggc ctggtaccgc cgaatttctg gacgctcaat ggcgtgcaga
     7861 tcggctcggc gctggagatg ttgttgctct cgctcgccct gggatcgcgc tatgccggcc
     7921 tgcgcatcga gaacatccgg atcgtgcagg aaaacaacga gcgactggaa cgcagcgtca
     7981 tcgaccgcac gcgtcagctg cgcacggcga tggcgcaact cggcgaggcc aacgtgcagt
     8041 tgcgcgaata cagccagcgc gacccgctga ccggcgtcta caaccgccgg cacttccgcg
     8101 aggcgctgtc gcaggcgctg gagctgcgcc aggccagcga ccagccggtc gccctgctgc
     8161 tgctcgacct ggaccatttc aagaagatca acgacaccca tggccacctg gtcggcgacg
     8221 actgcctgaa cgtggcggcg cgctgcatcg aggacgtcgt ggtgccgcgc ggcgggatcg
     8281 tggcccgctt cggcggcgag gaattcgtcg cggtgctgcc cggactggat gcgcagcagg
     8341 cgctgcagct cgccgaggcg gtgcgcctgc gcatccacca gacgccggtc gacagcgacg
     8401 gccgcagcat ccacctcagc gccagcatcg gcgtgcatac cgcgaccggg gaacgggcga
     8461 cgcctgaaga tgtgatccga ctcgccgacg aagccctgta ccgggccaag gacgacggcc
     8521 gcaactgcgt gcggcattcg gtcagcctgg tctgacgctc atttgcagcc agccggtccg
     8581 tggtgagtcc gtttgtcgtt ggtgatgcgc ccgtcgaacc atgagcgggc aaacgggagc
     8641 atgcgttgtg gttcgagaag cgcaccacca acggatgcgc ggccgacaag caagcctggc
     8701 cctcagccgc gccgcgattc cagcacgcgc ttcgcatcgg ccagcgacaa cccgcgcgcg
     8761 cgcaacagca cgatcaggtg gaacagcagg tcggcggctt cgccgagcaa cgcgtcgtcg
     8821 tcctgcacca ccccagccag cgcggtctcc acgccctcct cgcccacctt ctgcgcgatg
     8881 cggcgcacac cgccctcgaa caggcgcgtg gtgtaactgc ctgcgggacg atcgcgctcg
     8941 cgggtggcga ccaatccatc gagtgccgcc agcgtgtccg cgggcgcgtt cgggaaacaa
     9001 ctggcgcgtt gcaggtggca ggtcgggccg tgcgggcgcg cctgcaccag cagggtatcc
     9061 gcgtcgcagt cggtttcgat gtggacgaga tccagcacgt cgccggaggt ttcgcccttc
     9121 atccacaggc gctgcttgct gcggctgaag aaggtgacct tgccgctgtc gagcgtggcc
     9181 tgcagtgcct cgcgacccat gtagccgagc atcagcacgc gcagggtgtc cgcttcctgc
     9241 acgatcgccg gcaacaatcc gccctgcttg tcccaggcca aggcctcgat gtcgaagtcg
     9301 aagtcgatgg ttcgcgtcac aaccgcacct cgatgccctg cacgcgcagg gcctgcttga
     9361 gcgcggggat cgccaccgcg ccggaatgga agatgctcgc cgccagcgcg gcatcgacat
     9421 cggcctgccg gaacacctcg gtgaaatgcg cgggctcgcc ggcaccaccg gacgcgacca
     9481 gcggcacccc gcaacgggca cgcaccgcgc gcagctggtc gatgtcgtag ccgccgcgca
     9541 cgccgtcgct gcccatgcaa ttgagcacga gttcgccggc acccagcgct tgcgcctcga
     9601 ccacccagtc cagcgtgcgc ttgcccggcg cgcgcatcgc atccggatcg ccggtgtggc
     9661 tgcgcacgcg ccattcgccg tcggcgtcga gtaccgaatc gatgccgacc accacgcatt
     9721 gcacgccgaa ggcttcggcc agttcggaga tcagcgcggg gcgttcaagt gccggcgtgt
     9781 tgaccgacac cttgtcggca ccggccagca ggatcgcgcg tgcgtcgtcc acgcggcgga
     9841 tgccgccggc aacgcaaaag gggatgtcga tgacccgcgc cacccgctcc acccagccac
     9901 gatccacgct gcgcccctgc gggctggcgg tgatgtcgta gaacaccagc tcgtcggcac
     9961 cctggtcgcg gtagcgcatc gccagttcga cgatgtcgcc catgtcgacg tggtcgcgga
    10021 atttcacgcc cttgaccacg cggccctcgc gcacgtccag gcagggaatg atccgccggc
    10081 tcagcattgc agcgcccctg gcagcgacag ccgcccttcc agcaaggcct tgccgaggac
    10141 cgcaccggca caaccggccc cgcgtgcggc gaccacgtcg gccacgtcgc gcacgccgcc
    10201 gctcgcttgc agttgcaggc cgggatgcgc ggcacggatc tcccgataca gcgcgatgtt
    10261 cggacccgtc agcatgccgt cgcggtcgat gtcggtgcac agcaggtggc gcaagccgcc
    10321 atcggcgtaa cgccgcagca agtcgtcgag ggtttccgcg gcggtctcgg tccagccatg
    10381 caccggcagg cgccagatgc cgtcggcatc gcggcgcgtg tcgagggcga cggtgatccg
    10441 ctcgctgccg aattcgccga gccacgcgag gacgctggcg gaatcgcgaa ccgccagcga
    10501 cccgatcacc acgcgcgacg ccccggcatc gaggatcgcg gccacgtcgt cacgcgtgcg
    10561 cacgccaccg ccggtctgca cctgcaagct cgtggtcgcg gcgatgccgc gcagcagggg
    10621 ctgcaaggtg tagccgccgg ccttggcggc atccaggtcg accaggtgca gccacgccgc
    10681 gccggcatcg gcgtaccgtt gcgcgaaggc cagcgggtcc tcgccatagc gggtttcctc
    10741 ggcgaaatcg ccttgccgca ggcgcaccac gcggccctcg cgcacgtcga tggcgggata
    10801 gacgatgaaa ctcatgcgcc gttccattgc aggaagttgg acaacaattg cgcaccgacc
    10861 gtggccgacc gctcggggtg gaattgcgca ccggccacgt tgccacgctg caccatcgcc
    10921 gcgaagcgct cgccatgcgt gcaggcggcg acgcaatccg cggtgaccgg cgcagcatag
    10981 ccatgcacga agtaggtact cgcgccatcc gcaacgccgt cgagcaaggc cgagtcgcgc
    11041 acgtgctcca ggccgttcca gcccatgtgc ggcacccgca agccaggacc gccgcgcagc
    11101 ttgcgcacgc gccccggcaa caggccaagg catgcgacgt cgccctcgtc ggacgactca
    11161 aaaaggagtt gcatgcccag gcagatgccc agcaacggca ccgtggccgc gcgcaacggc
    11221 tcgaccagtc cgcgctcgcg cagcagggcc atcgccggcg ccgctgcgcc aacccccggc
    11281 aggatgatgc gcccggcatc gcacaggcca tcgccatcac ggacgatgcg cgcctcaacg
    11341 ccaagtcgtt ccagcgcgta acgcaccgag ccgaggttgg caccgccggc atcgaccagg
    11401 gccagcgtgg tcacagggtg cccttggtcg atggcaattc agcgccttcg cgcttgatcg
    11461 cctggcgcag cgcgcgcgcc agcgccttga aggaggcctc gaccttgtgg tggtcgttgt
    11521 caccctgcac cttcaggttg aggttcagtc ccgatgcatc gcagaccgag cggaagaagt
    11581 gcgggaccag ttcggtcggc aggtcgccca cgcgctcacg cttgaactcg ccctcgaaca
    11641 cgaaatacgg ccgtccgctg aaatccagcg cggcactggc gagggtttca tccatcggca
    11701 gggtgaagcc gtagcgcccg atgccgcgct tgtcgcccag tgcttccttc agcgcctgcc
    11761 cgatcgcaag cccggtgtct tcgaccgtgt ggtgctcgtc gatgtgcagg tcgccgtcgg
    11821 tgcggacgtc cagcgcgaag ccgccgtgct tgccgatctg ttccagcatg tggtcgaaga
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acgtccagcg cgaagccgcc
    12001 gtgcttgccg atctgttcca gcatgtggtc gaagaacggc aggccagtgt ggacgcgcgg
    12061 ctcggcgacg cgatccaggt cgacctcgac gcggatcttc gtttccttgg tgttgcgctg
    12121 gaccaccgcg cggcgcggcg catccgcgag ttcgtgggcg atgccggccc agtcccactc
    12181 gccgccgaat tgcgcggtct tcagctggaa gccgcggatc ttgaggttgt ccgcgaactc
    12241 gatgtcggtg ggacggtcgc cgaccatccc ggaacgcgac cagtcgatgc cgcgatcctg
    12301 caggtacccc accatcatgc ccaggcccgg cttgcgggtc ggcttgttgt cgtgcgggaa
    12361 actctcgtcg atcagcactt cgcggaaacg gatgccctgg ctctcgaaca cctgcatcat
    12421 caggtcgtgc ggaccctgga agctttcgcg cggataggct tcggtgccca gtccgtcctg
    12481 gttgctgacg atgacgaact catagcccgc atcgcgcagt ttcagcatcg ccgggatcac
    12541 gtcccggacg aagcgcagct tcggataggc gtcgatctgg aaatcctccg gctcctcgat
    12601 cagggtgccg tcgcgatcga cgaacaggat cggcgtcccg ctcatgccgc cacctccacc
    12661 gcatccgcat ccagcgccgc cagcacgcgc cggcattcgt ccggcatgcc gacggtgatg
    12721 cgcagcgcgt cgccgagttg cggcatcgcc cgcatgtcgc gcaccaccac gccggcctcc
    12781 agcaattgcg cgaacgcggc atcggcatcg atgaaacgga ccagcaggaa attccccgac
    12841 gagggataca cgcgcagcac ctgcggcagc cggcgcaggc cgcccgccag ccgttcgcgc
    12901 cactcgcggg tttccgcgac gcgcgcggcg gtactggcca gcgcctgtgg cgccatggcc
    12961 ttgagcgcgg cctcggtcac cggttgcggc atcggatacg gcgcctggca gcgacgcaag
    13021 gcctcgatca gtgctggcgc ccccagcacg caacccacgc gcgccgccgc cagcgcatgc
    13081 gctttcgaca gcgtgcgcag cacggcgatg ttctcgtgcg cggggaacag cgccgtcgcc
    13141 gagggcacgt cggaatactc ggcatacgcc tcgtccacca ccaccagcgc cttgccgcgt
    13201 agccgcaccg cgagcgacgc gatctcgatc agcggcaagg cttcgccgga cggattgccc
    13261 ggcgtgcaca ggaagacgag gctcgcgccg ttggccagtg cggcatcgcc gagcgcctgc
    13321 aggtccaccc gcaggccctg ctcgccatcg accagcggca cgtcgatgac gcgcgcgcca
    13381 tgcaggcgtg cgcagaccgc atacatgccg aagaccggcg gcgcgatcgc gatggcgccc
    13441 tgccccggcg cgcagaacgc gcggaccagc aggtcgatgg cctcgtcgct gccacgaccg
    13501 accagcaatt gcgcgggctc gacgtcgtac agcgcggcca gccgcgagcg cagggcgcgt
    13561 ggctgcggat ccggatagcg gcgcagggtg gcgtcggcat cggaaggatt ggcccaggcc
    13621 gattcgttgg cattgagcca gacattgcca tcgaccaggt cgctgcgtgc ggacttgtaa
    13681 ccgccgaaat cgcgcaggtc cgcgcgcagg agcgcattca cggtcgcgtt catgccgcac
    13741 gctccaggcg cagttcgacc gcgcgcgcat gcgcctgcag gccttccgca tgcgccagcg
    13801 tcgccgcgca ggggccggca gcggcaatgc cggcgcggct ggcatcctgc acggtgatcg
    13861 ccttctggaa actggccacc gacaacccgc tccacgcacg cgcggcaccg ccggtcggca
    13921 gcacgtggtt gctgccgctg cagtagtcgc ccagcgcttc cggcgtccag tcgcccagga
    13981 aaatgctgcc ggcgttttgc acgcgatcca gccagtcgcg cggttcacgc acggccagga
    14041 tcaggtgctc gggtgcatat gcatcgctga cctcgatcgc ctgggcgatg tcgcccaccc
    14101 gcaccaaccg cgacgccgcc agggcctgcg tggcgattgc ggcacgcggt agccgggcaa
    14161 gctgcaactc gacttcgacg gccacggcat cgagcaacgc gtcgttgtcg gagacgagca
    14221 gcacctgcga atccgggcca tgttcggcct gcgacaacag gtcggacgcg acgaacgcgg
    14281 gatcggcgcc ggcatcggcg atcaccagga cttccgacgg accggcgggc atgtcgatgg
    14341 cgatcccgcc cggccgctgc aacgcctgcc gcttggcttc gtcgacccaa ctgttgccgg
    14401 gcccgaacaa tttgtcgcaa cggggcatgc tgccggtacc gagcgccatg gcggcgatcg
    14461 cctgcgcgcc acccagcttg aagacgcgta cgcccggcgc ctgttgcgcc gcgagcagta
    14521 ccgcgggatc ggcgctgccg tccttgcgcg ggggcgtgca caggatgatc tcgcgacagc
    14581 cagccagctg cgccgggatg gccagcatca gcgcggtcga tggcagtggc gcgctgcctg
    14641 cgggcacata caaaccgact cgcgcgatcg gccgctgcac gcgttggcac aacaaccccg
    14701 gcgcggtctc gagttcgtac ggtgcggcga cgccggcacg gtggaacgcc tcgatgcgcg
    14761 atgccgcttc ggcaatcgcc gattgcacgt cggctggcac cgcggcacgc gccgcggcga
    14821 attcatcggt accgacttcg aagtctgcca atgtcacacc gtcaaatttc gccgtgtagt
    14881 cgcgcaacgc atcatcgcca cgcgcggcga catcctcgaa gatcgcctcc acgcggtcgc
    14941 gtaccgcatc cgacaccgcc tgcaccgggc ggcgcagcac ggcttcgcgc tcgcgctcgt
    15001 ccagcgcgtt ccagtcgatc cgcttcatgc cagcatccgc tccaccggca gcaccatcag
    15061 cccgcgcgca cccgcacgct tgaggtcttc caggcgctgc caggtcatcg cgccgtggca
    15121 gagcgcctgc aacgcgagcg tgtcagcgcc gtcgaccgcg cagaccgtgg gcggttcggc
    15181 atcgggcagc aattgcagca gttggtccac ggcatcacgg ggggcctgga acatcagcag
    15241 cttgctgtcg cggatgcgca gcacgccgtc catgcgccgc agcagcaggt cggccagttc
    15301 cgcgcgcacg tcgccgaacg gttgcgccgg accggccagc acggcttcgc tgcgcatgat
    15361 gtcgagcacc ggcttgagct ggttggcggc cagggtggcg ccgctggaca ccaggtcgca
    15421 gaccgcatcg gcctggccaa ggcgcggcgc gatttccacc gagccattga gtaccaccgc
    15481 ctcggcatcg accccctgcg tgcgcagcca cgcgcgcaac tggttcgggt aggtggtcgc
    15541 gatgcgcttg cccgcgagtt gctgcgggcc ctgccagtcc caatcgtccg gcaccgccag
    15601 cgacagcttg cacccgccga agccgagcgc gcgccactcg cggaacacct cgggcttgcc
    15661 ggcttcggcg cgttcgccgg cctgctccgc cagcacgttg cggccgacga tgccgaggtc
    15721 gcagacgcca tccgcgatca ggcccgggat gtcgtcgtcg cgaaccagca gcaggtcgac
    15781 cggcagcgat tcgccgtagc agaacagctt gtcgcggctc tcgcgccagc tcagccccga
    15841 cgaggccagc agggcgcgcg ccggttcgct gaggcggccg gatttctgga tggcgatgcg
    15901 cagtcggtcg cgcaccgcgc tgttggcggt gg
//
LOCUS       DAOOCX010000048        15857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3322_length_15857_cov_5.861790, whole genome shotgun sequence.
ACCESSION   DAOOCX010000048 DAOOCX010000000
VERSION     DAOOCX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15857
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3322_length_15857_cov_5.861790"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PK399_06290"
     CDS             complement(<1..964)
                     /locus_tag="PK399_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPW12760.1"
                     /translation="MDVSTPSRRPRSAESCPWQADGRFAADLPLEDYPSTMLLRLAQA
                     IQQQVSATYARAHGLSVAEWRMLARLNSEGSVQLATLCRALAMDKAYAGRLLRSLGAQ
                     SLVTVEADPAHGRRLMVAITPAGRALARRILPQARASQEQLLQVLDPQERAALYASLK
                     KLQAALDDTAPQPASHATEQPAQQLAARLFEVLRGQPHAHGGEEILDVPGVEFVAVGL
                     ADAVDQPHLQRDDRHGRQRLRQQFQRAVGDGGVEDGLVELLLADVGRAHAHAHASHAR
                     RGHGRRLDTAARRRHRAGHGRGGDGFGVFGELPRPARIQAQGAAQ"
     gene            complement(1041..1475)
                     /locus_tag="PK399_06295"
     CDS             complement(1041..1475)
                     /locus_tag="PK399_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPW12761.1"
                     /translation="MTEAPALGDPTLDADHAKLADLVDRLAAAKGADVLASLDALQAH
                     ASEHFALEDQELRQMRDGNASCHLDEHAAVLKSMDEVRGIVAAGPDSTESAHLVARLT
                     AELRRWLPEHVTAMDAGVAHTRTRSRLGGAPVVLTRRSSPPG"
     gene            complement(1541..2320)
                     /locus_tag="PK399_06300"
     CDS             complement(1541..2320)
                     /locus_tag="PK399_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW12762.1"
                     /translation="MDMPTGNKSTVNFDFHGRTALVTGAGRGVGEEIARLFHASGARV
                     AVSDIDGQAARQLADRLDDSGVSAMALTLDVRSKRAFEDARDRIASAWGRTDIVVNNA
                     GYAKRTPVQDITTEEFDDIVAINMRSVFLSCQVFADHMRSNGWGRIVNITSLAGQNGG
                     TVASAHYAAAKAGATMLSKYFAQQLAGTGVTVNAIAPGPISTAKGRLSQEQIERIEKL
                     VPIGRFGEASEFAAAVALLASDQGGFFVGATLDMNGGLFMR"
     gene            complement(2321..3250)
                     /locus_tag="PK399_06305"
     CDS             complement(2321..3250)
                     /locus_tag="PK399_06305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HPW12763.1"
                     /translation="MKARVRSVRWEAQDILGFRLEPLPGAHLAPFTAGAHVDVALRPG
                     LTRSYSLLNDPREHGAYEIAVQLDPATRGGSRHIHEEWRAGQLVEVSEPRNNFPLEEN
                     SAHTVLVAGGIGITPMLSMIARLQRLGSSWELHYAVRTRARAAFLDQLAAFAQVHVTI
                     DDEPSTPRLDLQRLLGSLAPQAHAYCCGPAGMLAAYREHGAHLGKRLHFEYFSADTQP
                     ACQGGYRLELQRSGKTLEVKAGETMLDALLNAGVDVGFACTEGVCGSCRVPVLHGVPD
                     HRDVFLTQDERDANDAVMVCCSGARTPSLTLDL"
     gene            complement(3247..4509)
                     /locus_tag="PK399_06310"
     CDS             complement(3247..4509)
                     /locus_tag="PK399_06310"
                     /inference="COORDINATES: protein motif:HMM:NF039910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HPW12764.1"
                     /translation="MGEFMRQYWIPAALSSELQADGDPMRLMLLGEKLVAFRDSQGCV
                     GIFDHRCPHRCASLYLGRNEESGIRCVYHGWKFDVRGNCVDMPNVPARQEFKDKVHAK
                     AYRTTERNGLVWVYMGRQQESPPPLPNIEANLIPGNEIWCLQRECNWLQALEGDIDTS
                     HVGFLHVGMLKPEDLDDDHPMRPTVINRAPEYEVRNTDWGTMYGGFRTNDDGQMSWRV
                     AHFMYPFWTQTPNARFSSRAIARAWVPMDDHHSMLFDISGGVDEGNPAYVSKRRNGQP
                     LYEKFEYEPNTNDWHGRWRCRDRAHNDWGLDRQSQRDGTQYTGIGNITIQDQAVTESM
                     GPITDHDWEHLAPTDQMIARTRRRLLLAARAYRDSGELPPGASDPDTFMGARAGSFLF
                     APDAPLEQAYQAQLEKAVRWTAQPEERA"
     gene            complement(4766..5116)
                     /locus_tag="PK399_06315"
     CDS             complement(4766..5116)
                     /locus_tag="PK399_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12765.1"
                     /translation="MPRSLAQNIKQVRDKIGGVRAALADIEYLCSGTLHMRMKVCGKP
                     GCRCAQDPQARHGPYYEWGHMIGGKLVHRMVTPAQAELLQHAIDNHRRAKQLMREWEE
                     QTERLIDLEVPRQP"
     gene            5161..6876
                     /locus_tag="PK399_06320"
     CDS             5161..6876
                     /locus_tag="PK399_06320"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HPW12766.1"
                     /translation="MPSSQAPTSTKEAAGFTLHTERLGPLPLINHFIERIGLHDTLSR
                     HIPSDARCSITHASALGVLLRSIIVEREPIYRQHETVHGFADGMFGISAQEMEHLSDD
                     RLGRALDRLFDADRTALLTELVLAVGQRFGVRFEEFHNDSTSISFCGGYRRARGRQIR
                     ARTAPAITFGHSKAHRPDLKQLLFILTMSADGNVPVAFRCTDGNTSDSVTHVQTWETL
                     RAVAGRSDFLYVADSKLCSRENMDHIDRAGGRFVTVMPRSRLEDAQFREWLQTHTPEW
                     SLVWDRPNPRYSDGPRDCWYVHRAPLPSMEAWSIVWVWSTLLTLRQHARRLRNIAAAI
                     EEFERLRQRLASAKTRLRGAPEIDLQIKLIVDRHHVGRYLKVRRVVREEHVFKQTRRG
                     RPGPDTAYRKITKRRFDVQWSTDEQAIAYDRRSDGMYPLMTNDRKLSAAQVLQAHKGQ
                     PMIEKRFEQIKTVHQIAPVFLKDEGRIEALFTLYAIALLVQALIERELRQAMAHEAIE
                     ELPIYPEQRQCAHPTTEQVLRLFSLAERHQLRQHGRTVQAFNLTLTDLQRKVLALLGV
                     PASTF"
     gene            7054..8316
                     /locus_tag="PK399_06325"
     CDS             7054..8316
                     /locus_tag="PK399_06325"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPW12767.1"
                     /translation="MNHAQSPPDAQPGAAQAAFQLNLRHLRGLTAVHEHGSISAAAGA
                     VGLSQPALTQGILKLEKLMGEVLFERRPDGIVPTAAGELVVDRARACLAHLATGTRQV
                     GGTAFEPDRRLSMTQLRAFVALVRAGSISAAAADLGLSQPAVHRAVRELEEALGRKLV
                     ERRGRGVHVNFAGRRFARSCRLALAELQAAFSELGRDPHNPTIALGTTPLARGFLVPE
                     AMALMVAERFPAGFRVHEGSWGELVEALRDGVIDIIVGEIPDDAAPDLATHPLYEEAP
                     VVVAGRQHPLATRRSCTPQMLASYPWIIAPENSPLRAEWERLFADRRPEAPVECGSIM
                     IIGRLLTASDLLTLAMPDQVALQVRTGLLSCIGAPLAGSRPTVGATMRKSWRLTRAQQ
                     RFLELLTVASAGIGADASRQSLIEPYWT"
     gene            8454..9746
                     /locus_tag="PK399_06330"
     CDS             8454..9746
                     /locus_tag="PK399_06330"
                     /inference="COORDINATES: protein motif:HMM:NF039910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HPW12768.1"
                     /translation="MLNSQDNETLVRVGPGTAMGQLMRLYWIPFLASADVERDGQPHR
                     VRLLGEDLVAFRDSEGQVGLVDQACPHRGAPMVFARNEEGGIRCIYHGWKFDVTGQCQ
                     ETPAEPPGSAMTSRMTIKSYPVRERNGVLWTYMGPKRETPPPLPELEWNMVPESHSVV
                     TFRVQECNWLQALEGEVDSAHAAILHGRRNGSTIAQWRQGQDLSPKFEVQPHDAGISI
                     AARRKDGTEHNYVRVNQFLMPFWTLVPPQTQYPELSGHAWVPIDDHHTLAIMFSYTPD
                     QPFYEKSRKLFKDGYKGRETGHHSHGAYEARPVTVPYHRYWSRFNRGNAYNYDYQTGM
                     PGLWLEDAACQSGVATICDRSQEHLGSTDAGIVRVRRLLLESAKKLAEGAEPVSAAKP
                     SSFMMRAISVTIPAGSDWMELGRDYMKAELGKGFGYTP"
     gene            9743..11098
                     /locus_tag="PK399_06335"
     CDS             9743..11098
                     /locus_tag="PK399_06335"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPW12769.1"
                     /translation="MSGSPMQAGGQDMLENLEADFPEHKRRASGAPHALDGIRVVDFS
                     HFIAGPLATMILADMGAEVIKVEAPVRGDDVRRYPPMHPQLDQGAPFVWSNRNKKSIA
                     LDLKSPQGLALARDLVCRADVLVENFSSGVMRRLGLDHETCLALNPRLVYCSVAAYSR
                     EGAFADRFGFDPIAQAESGFVSMNGYPDREGVRALSPVMDISTAMMVGNAVLGALLAR
                     ERLGRGQRVEVSLFDTAVLMTGYATMQSLFTGKEPQRSGNTAPDTCPSGAFRASDRTF
                     YINSGNDRIFQRLMGQVLERPDVAADADYATGPLRIRHRERLFAFLDEAFARHDWAHW
                     QARMRAAGVPYGLVRTVGEAIRSPEARDHELVTRIPHPVLGWVPNVRLPIRYAATPMA
                     DPVAAPAVGQHTQEVLRDALGLDERQIAELERAGAFGPAAAAAAAAVPGPMDRDPVPA
                     S"
     gene            11154..12140
                     /locus_tag="PK399_06340"
     CDS             11154..12140
                     /locus_tag="PK399_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HPW12770.1"
                     /translation="MTLHTRRSALRMLAAGGAAASVGLPAFSQQGEAFPSRVIRIVVP
                     YPAGGTTDQLARAIAQPMREVLGQTVIVENKPGAGGTIGTDYVAKQPADGYTLVFGNS
                     GPSATAGLMRKLPYDPQKDFRPISGVMRVPMILAVPADSPHKSVAEFVAWARSRGDKV
                     NYGSTGVGGGSHLYSEYFNELAGTRFQHIPYTGAPPLLTAFAGGQVQMAFVTGLDGAA
                     MVQAGKVRYLAVASPKRTPVLPDLPAIAEAVPGFQAVGWFGVLAPAGVPDPVAQKLSS
                     TIAAAVRRPEVSKFLTDRNVEPWGSTQEELGRTIEGEIAQWGPLVKKFNITV"
     gene            12154..13350
                     /locus_tag="PK399_06345"
     CDS             12154..13350
                     /locus_tag="PK399_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12771.1"
                     /translation="MAQRTLKGRVAIVGIGETHYYKHGQSPDAEFKLALQAVLRACAD
                     AGLDPRHIDGFASYGDDRSEATRLASALGLPRLRSATMQWGGGGGGCCAAVANGAAAI
                     ATGQADCVVVFRSLNQGQYGRFGQSAGSGTISGERAYLVPYGVISPPQRFAMRIQRYM
                     HEHGIRQEALRAIALASYHHAQANPRAVMHGKPLDAEKYDASRWIAEPLHLFDCCMEN
                     DGAAAIVMVDAERAADFAHKPVYLLGAASGSSHRFATHAHNSPSYASANFETVAEDLY
                     RMAGLGPADVGVVQSYENFTGGVVMALAEHRFFSPQEANDFLTLENLIAPSGRLPLNT
                     SGGNLAECYMHGFELVLEAVRQVRGTSTAQAARSDVALVIGGPMAAPVSNLLLGSRDA
                     LGETPR"
     gene            13347..13793
                     /locus_tag="PK399_06350"
     CDS             13347..13793
                     /locus_tag="PK399_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HPW12772.1"
                     /translation="MTMPSSYLPPGLPIPVPEPDGVSAPYWNGLREGVLRVQRCAHCQ
                     AWQFGPEWICHACLQFDPAWVEVEPSGLIFSWERIWHPAHPALKDHGAYLAVVVELPH
                     AGRVRMVGNLLGDPLQQVRIGAEVQGVFEHHPEASQPYTLLQWRCR"
     gene            13790..15529
                     /locus_tag="PK399_06355"
     CDS             13790..15529
                     /locus_tag="PK399_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW12773.1"
                     /translation="MTGSSSTDERQADIDLVVVGAGAGGMVAALVAALEGLRVVLCEG
                     SQQVGGTTATSAGTLWIPGNRHGAEAGHADSVQAAASYLDALIGPDDGRQLRQAFLES
                     GDAAIAYLEERTDVAFVSAGQHPDYLALPGAAIAGRALAPRPFDGRLLRGAFARIRPP
                     MPEFLLLGGMMVGKADIQALVRRWQSWPAFVASAGLVARYLVDRIRYPRGTRLVMGNA
                     LVARLFYSLQRAGVDVRFGWRLRELTVEGGKVRGATFAVDGQERRLDSRAGVVLATGG
                     VGHGEALRAELAPAGMSLPSLACDAVNGEGITAARSAGAGFERYAAGDFFWQPVSRVP
                     RPGGPGLFPHLFLDRAKPGLVAVDAQGRRFADEAGSYHHFVEAMVRHLQRTGGDAAWL
                     VCDAAFVHKYGLGVILPGTRRLDRWIRQGYVCVADRLPALAQRAGIDPAGLLATVQRT
                     NDYAATGRDPDFHKGDAPVDRFNGDPAHRPNPCLGEIGQPPFVALRILPADAASSSGL
                     PIDADGRVLDRAGRVVVGLYACGNDAASVMRGTYPGPGTTLGPAFVFAWRAALFAARR
                     AASARAHPIEEKP"
     gene            15529..>15857
                     /locus_tag="PK399_06360"
     CDS             15529..>15857
                     /locus_tag="PK399_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPW12774.1"
                     /translation="MEPNASPARRAGPLADLRVVEFAGIGPGPFACMLLSDMGADVVT
                     IDRPGARASDPRNVTQRGRTVVQADLKDAACIEQVLQLLQAADVLVEGFRPGVMERLG
                     LGPEAVAR"
BASE COUNT         2418 a         5463 c         5465 g         2511 t
ORIGIN      
        1 actgcgcagc accctgggcc tggatgcggg ccgggcgcgg cagttcaccg aagacgccga
       61 atccgtcgcc gccgcgccca tggccggccc ggtgccggcg ccgggcggca gtgtcgagcc
      121 tccgcccgtg cccacggcgg gcgtggctgg cgtgggcgtg ggcgtgcgca cgcccaacgt
      181 cagcgagcag gagctcgacc agaccatcct caacgccgcc atcaccaacg gcgcgctgga
      241 actgctgccg gagacgctgt cgaccatggc gatcatcccg ctgcagatgc ggctggtcta
      301 ccgcatcggc caggcctacg gctacgaact cgactccggg cacgtcaagg atttcctcgc
      361 caccgtgggc gtggggttga cctcgcagta cctcgaacag gcgggccgca agctgctggg
      421 cgggctgctc ggtcgcgtgg ctggcgggct ggggcgcggt atcgtccagc gcggcctgca
      481 gcttcttcag cgatgcgtac agcgcggcgc gctcctgcgg atccagcacc tgcagcaact
      541 gctcctggct tgcgcgcgcc tgcggcagga tgcggcgcgc cagcgcgcgg ccggcggggg
      601 tgatcgccac catcagccgg cggccgtggg ccggatcggc ttcgaccgtg accagcgact
      661 gtgcgccgag ggagcgcagc agccgccccg cgtacgcctt gtccatcgcc agcgcgcggc
      721 acagcgtggc cagttgcacg gagccttccg aattcagccg tgccagcatg cgccactcgg
      781 ccaccgacag gccgtgcgca cgtgcgtagg tggcgctgac ctgctgctgg atcgcctggg
      841 cgagccgcag cagcatggtg ctgggatagt cctccagcgg caggtcggca gcgaagcgac
      901 cgtcggcctg ccaggggcag gactcggcgg agcggggccg gcgggacggt gtggacacgt
      961 ccacactgtg atccgggccg ggcggcgacc ggagagagtt ttccctgcgg tcctaggtat
     1021 gcgtggtgag gtggcgccga tcagcccgga ggcgaagacc ggcgagtgag caccaccggc
     1081 gccccaccca ggcgactgcg ggtgcgggta tgcgcgacgc ctgcatccat ggccgtcacg
     1141 tgctcgggca gccagcggcg cagctcggcc gtcagccgcg ccaccagatg ggcggactcg
     1201 gtcgagtccg gaccggccgc gacgatgccg cgcacctcgt ccatcgactt gagcacggcc
     1261 gcgtgctcgt ccaggtggca gctggcgttg ccgtcacgca tctgccgcaa ttcctggtcc
     1321 tccagcgcga agtgttcgct ggcgtgcgcc tgaagcgcgt cgaggctggc gagcacgtct
     1381 gcgcctttgg cagcggcgag gcggtcgacg aggtcggcca gcttcgcgtg gtcagcatcg
     1441 agtgtgggat ctccgagagc gggtgcttct gtcatggctg ggcggcgtcg agctgcaatt
     1501 caggaatcgg gagtaaggcg ggaacgatag ccggcttttc tcagcgcatg aagagcccgc
     1561 cgttcatgtc cagagtcgcc ccgacgaaga acccgccctg gtcggacgcc agtagcgcca
     1621 ccgcggcggc gaactcggag gcttctccga atcggccgat cggcaccagc ttctcgatgc
     1681 gctcgatctg ttcctgcgac agccgcccct tggccgtgga gatcgggccc ggcgcgatgg
     1741 cgttgacggt gacgccggtg cccgcgagct gctgtgcgaa gtacttcgac agcatggtcg
     1801 cccccgcctt ggctgccgcg tagtgcgccg acgcgaccgt gccgccgttc tgcccggcca
     1861 gcgaagtgat gttgacgatg cggccccagc cgttggagcg catgtgatcg gcaaagacct
     1921 ggcagctgag gaagacgctg cgcatgttga ttgccacgat gtcgtcgaac tcctcggtcg
     1981 tgatgtcctg caccggcgtg cgcttggcgt aaccggcatt gttgaccacg atgtcggtac
     2041 ggccccaggc gctggcgatg cggtcccgcg catcctcgaa cgcccgtttg ctgcgcacgt
     2101 cgagtgtcag ggccatcgca gagacgccgg aatcgtccag gcgatccgcc agctgccgcg
     2161 cggcttgccc gtcgatgtcg ctcaccgcga cgcgcgcgcc ggaggcgtgg aacaggcggg
     2221 cgatctcctc gcccacgccg cggccggcgc cggtgaccag tgccgtgcgg ccgtggaagt
     2281 cgaaattcac ggtgctcttg ttgccggtcg gcatgtccat ctagaggtcc agggtaaggg
     2341 acggggtgcg tgccccggaa cagcacacca tcacggcgtc gttcgcatcg cgttcgtcct
     2401 gggtgaggaa gacgtcgcgg tggtccggca cgccatggag cacaggcacg cggcagctgc
     2461 cgcagacgcc ttcggtgcag gcgaagccca cgtcgacacc ggcgttgagc aacgcgtcca
     2521 gcatggtctc gccggccttg acctcgaggg tcttgccgct gcgctggagt tccagccggt
     2581 agccgccctg gcatgcaggc tgcgtgtccg ccgagaaata ctcgaagtgc aggcgcttgc
     2641 ccaggtgggc gccatgctcg cggtacgcgg ccagcatgcc ggcgggcccg caacagtagg
     2701 catgcgcctg cggcgccagc gagccgagca ggcgctgcag gtcgagccgc ggcgtgctcg
     2761 gctcatcgtc gatggtcaca tgtacctgcg cgaatgccgc cagctggtcg aggaaagctg
     2821 cacgggcccg cgtgcgcacg gcgtaatgca gctcccagga agatcccagg cgttgcagtc
     2881 tcgcgatcat cgacagcatg ggcgtgatgc cgatgccgcc ggcgaccagc acagtgtgag
     2941 ctgagttctc ctccagcgga aagttgttgc gcggttccga gacttccacc agctgcccgg
     3001 cacgccattc ctcgtggatg tggcgcgagc cgccgcgggt ggcggggtcc agttgcacgg
     3061 cgatctcgta ggcgccgtgc tccctggggt cgttcagcag ggaatagctg cgggtgaggc
     3121 caggacgcag cgccacgtcg acgtgggcac cggcggtgaa gggtgccagg tgcgccccgg
     3181 gcagtggctc cagccggaag cccaggatgt cctgtgcctc ccagcggacc gatcgaaccc
     3241 tcgccttcat gcccgctcct cgggctgcgc cgtccagcgc accgcctttt ccagttgcgc
     3301 ctggtaagcc tgttccagcg gcgcgtcggg ggcgaacagg aaggagccgg cgcgtgcgcc
     3361 catgaaggtg tccgggtcgc tggcgcccgg cggcagttcg ccgctgtcgc gataagcacg
     3421 ggccgcgagc agcaggcgac ggcgggtgcg ggcgatcatc tggtcggtcg gcgcgagatg
     3481 ttcccagtca tggtccgtga tgggccccat gctctcggtc accgcctggt cctggatggt
     3541 gatgttgccg atgccggtgt attgcgtccc gtcgcgctgc gactggcgat ccaggcccca
     3601 gtcgttgtgc gcccggtcac ggcagcgcca gcggccatgc cagtcgttgg tgttcggctc
     3661 gtactcgaac ttctcgtaca gcggctgccc gttcctgcgc ttggacacgt aggcagggtt
     3721 accttcgtcg accccgcccg agatgtcgaa cagcatgctg tggtggtcgt ccatcggcac
     3781 ccaggcgcgc gcgatggcgc ggctggagaa gcgtgcgttg ggcgtctggg tccagaacgg
     3841 gtacatgaag tgggcgacgc gccaggacat ctggccgtcg tcgttcgtgc ggaatccccc
     3901 gtacatcgtg ccccagtcgg tgttgcgcac ctcatactcg ggagcgcggt tgatgacggt
     3961 ggggcgcatc ggatggtcgt cgtcgaggtc ctcgggcttg agcatcccca cgtgcaggaa
     4021 acccacgtga gaggtgtcga tgtcgccctc cagcgcctgc agccagttgc actcccgctg
     4081 caggcaccag atctcgttgc cggggatcag gttggcctcg atgttgggca agggcggggg
     4141 cgattcctgc tgccggccca tgtacaccca caccaggccg ttgcgttcgg tcgtgcgata
     4201 ggccttggca tgcaccttgt ccttgaattc ctggcgcgcc ggcacgttgg gcatgtccac
     4261 gcagttccct cgcacgtcga acttccagcc gtggtagacg cagcggatgc cgctttcctc
     4321 gttgcggccc aggtagagcg aggcgcagcg atgcgggcag cggtggtcga agatgccgac
     4381 gcatccctgc gagtcgcgaa aggcgacgag cttctcgccc agcagcatca gccgcatggg
     4441 atcgccgtcc gcctgcaact cggaggagag cgccgcgggt atccagtact gccgcatgaa
     4501 ctctcccatg acggttccgg gacccacccg cgtcaggtcg gtgctgtctt gcgctttcat
     4561 gaatgccctt gccgcggtgc atgccgcgat ggatggtgag gggcccggag acttccgggc
     4621 cagtgcactg atcgtaggag cagacgcggc gccgcgtgat tcgaagcgcg ccggcgacat
     4681 tcaataaccg gaaggatcgg cataccggct tactttccgc aggtcgccag acgcatttgc
     4741 gcgtaatttc ccggtcgcgg gaaggtcaag gttggcgcgg tacttccaag tcgatgagac
     4801 gctcggtctg ctcctcccac tcacgcataa gctgcttggc acggcggtgg ttgtcgatcg
     4861 cgtgttgtag aagctcggcc tgcgctggcg tgaccatgcg atgcacgagc ttgccgccga
     4921 tcatgtgccc ccattcgtag tacgggccat gacgggcctg cggatcctgg gcgcaacggc
     4981 acccgggctt gccgcacacc ttcatgcgca tgtgcaacgt gcccgagcac aggtactcga
     5041 tgtcggccag ggccgcccgg acgccgccga tcttgtctcg gacctgcttg atgttttgtg
     5101 cgagtgaacg cggcatgagt gcttgacttt tgcgattctg tcgttaatag tagcgccaga
     5161 atgccaagct cgcaagcccc gacttccacc aaggaggcgg caggcttcac tcttcacacc
     5221 gagcgcctgg gcccgctgcc cctgatcaac cacttcatcg agcgcatcgg cctgcacgac
     5281 accctgtcgc gccacatccc ctcggacgct cgctgcagca tcacccatgc cagcgcgctg
     5341 ggcgtgctgc tgcgatcgat catcgtggag cgcgaaccga tctaccgcca gcacgagacc
     5401 gtgcacggct tcgccgatgg catgttcggc atcagtgccc aggagatgga gcacctgagc
     5461 gacgaccgcc tcgggcgcgc cttggaccgg ctgttcgatg ccgatcgcac ggcgttgctg
     5521 actgagctcg tgttggccgt tgggcagcgc ttcggggtgc gtttcgagga gttccacaac
     5581 gactccacat cgatcagctt ctgcggcggc taccgccgcg cccgaggccg ccagattcgc
     5641 gctcgcaccg cgcccgcgat caccttcggg cattcgaagg cgcacaggcc ggacctcaag
     5701 caactgctgt tcattctgac catgagcgcc gacggcaacg tgccggtggc gtttcgctgc
     5761 accgacggca acaccagcga ctcggtcacc cacgtgcaga cctgggagac gctcagggcg
     5821 gtggccggca ggagcgactt cctgtacgtg gccgacagca aactgtgttc gcgcgagaac
     5881 atggatcaca tcgaccgcgc agggggccgc ttcgtcaccg tgatgccacg cagccggctt
     5941 gaggatgcgc agtttcgcga atggctgcaa acccacacgc ccgagtggtc cctggtgtgg
     6001 gatcggccca acccgcgcta cagcgacggg ccgcgcgact gctggtacgt ccatcgggcg
     6061 ccgctaccct ccatggaagc ctggtccatc gtgtgggtct ggagcacgct gctgacgctg
     6121 cgccagcatg cccggcggct acgcaacatc gccgctgcca tcgaagagtt cgagcggttg
     6181 cgccagcgcc tggcctccgc caagacccgg ctgcgcggcg cccccgagat cgacctgcag
     6241 atcaagctca tcgtggacag gcaccatgtg ggtcgctacc tcaaggttcg ccgcgtcgtt
     6301 cgcgaagagc acgtcttcaa gcaaacccgc cgtggccgcc cgggccccga cacggcctac
     6361 cgcaagatca ccaagcgacg cttcgacgtg caatggagca ccgacgagca ggccatcgcc
     6421 tacgaccggc gaagcgacgg catgtacccg ttgatgacga acgaccgcaa gctcagcgcc
     6481 gcacaggtcc tgcaggccca caaaggccag cccatgatcg agaagcgctt cgagcagatc
     6541 aagaccgtac accagatcgc cccggtgttc ctcaaggacg agggtcgcat cgaggcactg
     6601 ttcaccctgt acgcgatcgc actgctggtg caggccctca tcgagcgcga gctgcgccag
     6661 gccatggcgc acgaagccat cgaggaactg cccatctacc ccgagcagcg ccagtgcgcg
     6721 catcccacca ccgagcaggt gctgcgcctg ttcagcctgg ccgagcgcca tcaactgcgt
     6781 caacacggcc gcaccgttca ggcattcaac ctcacactca ccgacctgca gcgcaaagtg
     6841 ctcgcgctgc tcggcgtccc cgcaagcacg ttctaggcac ctcgaaacgc agcccggaaa
     6901 ttacgcgcaa atgcgtctgc tgacccgcgg aaagtaagta ccggcgcctc agcgccaagg
     6961 gtggcggcac cggcggtcaa ttcaacaagc ggaagccccg acgagggtgc gctttatagt
     7021 ggtgcctggc cagcttcctc ttccttggcg accatgaacc acgcgcagtc tccgccggac
     7081 gcgcagcccg gcgcggcaca ggcggccttc cagctgaacc tgcgccactt gcgcggcctg
     7141 acggccgtgc acgagcacgg cagcatcagt gcggccgctg gcgcggtggg gctcagccag
     7201 ccggcgctga cgcagggcat cctgaagctg gagaagctga tgggagaggt gctgttcgag
     7261 cggcgcccgg acgggatcgt gcccacggcg gccggggaac tggtggtgga ccgggcgcgg
     7321 gcctgcctgg cccacctggc aacgggcacc cgccaggtgg gcggcacggc gttcgaaccg
     7381 gatcgccggc tcagcatgac gcaactgcgc gccttcgtgg cgctggtgcg ggccggcagc
     7441 atctccgctg cagccgcaga ccttgggctg tctcagccgg cagtgcaccg ggccgtgcgc
     7501 gaactcgagg aggcgctggg acgcaagctg gtggagcgcc gcggccgcgg agtgcacgtc
     7561 aactttgccg gtcggcgttt tgcccgcagt tgcaggctcg cgctggccga actccaggcg
     7621 gcgttctccg agctcgggcg cgacccgcac aaccccacca tcgctctcgg cacgaccccc
     7681 ctggcacgtg gcttcctcgt gcccgaggcc atggcgctga tggtggcgga acggtttccc
     7741 gcggggttcc gcgtgcacga aggcagctgg ggcgaactgg tggaggcgct cagggacggt
     7801 gtgatcgaca tcatcgtcgg cgagatcccc gacgacgcag cgccggacct ggccacgcac
     7861 ccgctttacg aggaagcccc ggtggtggtg gcggggcggc agcatccgct cgcgacgcgg
     7921 cgcagttgca cgccgcagat gctggcttcc tacccctgga tcatcgcgcc ggagaactcg
     7981 ccgctgcgtg ccgaatggga gcgcctcttc gccgatcgcc gccccgaggc gccggtggag
     8041 tgcggctcga tcatgatcat cggccgcctg ctgaccgcca gcgacctgct gacgctcgcc
     8101 atgccggacc aggtggcgct gcaggtgcgc accggcctgc tcagctgcat cggcgcaccg
     8161 ctggcgggaa gcaggccgac ggtcggcgcc acgatgcgca agagctggcg cctcacgcgg
     8221 gcccagcagc ggttcctcga actgctgacg gtggcttccg ccggcattgg cgccgacgcg
     8281 agccggcagt cgctcatcga gccctactgg acctgaaggc cgctgccacc actgtggggc
     8341 cagctcaatt ccggtttctc aataccgcgg cggcctttcg aatcacgcgc cggccggcac
     8401 tgcctagaat cgggcgaatc gatgttcggt tcaaccactc accctaaaag gccatgctga
     8461 attcccaaga caacgagacg ctcgttcgcg tcggccccgg taccgcgatg ggccagctca
     8521 tgcggctgta ctggatcccc ttcctcgcgt ccgccgatgt cgagcgcgac ggccagccgc
     8581 atcgggtgcg cctgctgggc gaggacctgg tcgcgttccg cgacagcgag ggccaggtcg
     8641 gcctggtgga ccaggcctgc ccgcaccgcg gcgcgcccat ggtgttcgcc cgcaacgagg
     8701 agggcggcat ccgctgcatc taccacggct ggaagttcga cgtgacgggg cagtgccagg
     8761 agaccccggc ggaacccccg ggcagtgcca tgaccagccg catgaccatc aagtcctatc
     8821 ccgtgcgcga gcgcaacggc gtgctgtgga cctacatggg cccgaagcgc gagacgccgc
     8881 cgccgctgcc tgagctcgaa tggaacatgg tgcccgagtc gcactccgtc gtcaccttcc
     8941 gggtgcagga gtgcaactgg ctgcaggcac tggaaggtga agtcgactcc gcgcatgccg
     9001 ccatcctgca tggccgccgc aacggcagca cgatcgcgca gtggcggcag ggccaggacc
     9061 tgtcgcccaa gttcgaggtc cagccgcacg atgccggcat cagcatcgcg gcgcggcgca
     9121 aggacggcac cgagcacaac tacgtgcggg tcaaccagtt cctgatgccg ttctggacct
     9181 tggtgccgcc gcagacgcag taccccgagc tgagcggcca tgcctgggtg cccatcgacg
     9241 accaccacac cctggcaatc atgttctcgt acacgccgga ccagcccttc tacgagaagt
     9301 cgcgcaagct gttcaaggac ggctacaagg gccgcgagac cggccaccat tcgcatggcg
     9361 cctatgaggc caggccggtg acggtgcctt atcaccggta ctggagccgg ttcaaccggg
     9421 gcaacgccta caactacgac taccagaccg gcatgcccgg cctgtggctg gaggatgccg
     9481 cgtgccagtc gggcgtggcg accatctgcg accgttcgca ggagcacctg ggctccaccg
     9541 atgcaggcat cgtgcgcgtg cgccggctgc tcctggagag cgcgaagaag ctggcggagg
     9601 gcgccgagcc ggtctccgcc gcgaagccgt cttccttcat gatgcgggcc atctccgtca
     9661 ccatccccgc cggttccgac tggatggaac tgggacgcga ctacatgaag gcggagctcg
     9721 gcaagggctt cgggtacacg ccgtgagcgg ttctccgatg caggcaggcg gccaggacat
     9781 gttggagaac ctcgaagccg actttccgga gcacaagcga cgggcctccg gcgcacccca
     9841 tgcgctggac ggcatccggg tggtggactt ctcgcacttc atcgcggggc cgctggcgac
     9901 catgatcctg gccgacatgg gcgccgaggt gatcaaggtc gaggcgccgg tccgcggcga
     9961 cgacgtgcgc cgctatccgc ccatgcaccc gcaactggac cagggcgcac ccttcgtctg
    10021 gagcaaccgc aacaagaaga gcatcgcgct ggacctgaag tccccgcagg ggctggcgct
    10081 ggcgcgcgac ctcgtctgcc gcgccgacgt gctggtcgag aacttctcct cgggcgtgat
    10141 gcgtcgcctg gggctggacc acgagacctg ccttgccctg aacccgcgcc tggtctactg
    10201 ctcggtggcc gcctacagcc gcgaaggcgc cttcgccgac cgcttcggct tcgatcccat
    10261 cgcgcaggcc gaaagcggct tcgtttccat gaacggttat cccgaccgcg aaggcgtgcg
    10321 ggcgctctcg ccggtgatgg acatcagcac cgccatgatg gtgggcaacg ccgtgctcgg
    10381 cgcgttgctg gcccgggagc gcctgggccg cggccagcgg gtggaggtgt ccctgttcga
    10441 cacggccgtc ctgatgacgg gctatgcgac catgcagtcg ctgttcaccg gcaaggagcc
    10501 gcaacgcagc ggcaacaccg cccccgacac ctgcccctcg ggcgccttcc gcgccagcga
    10561 ccgcaccttc tacatcaaca gcggcaacga cagaatcttc cagcggctca tggggcaggt
    10621 gctggaacgt ccggacgtgg ccgccgatgc ggattacgcc acgggtcccc tgcgcatccg
    10681 gcatcgggag cgcctgttcg ccttcctcga cgaagcgttc gcgcggcacg actgggcgca
    10741 ctggcaggct cgcatgcgcg ccgccggcgt gccctacgga ttggtgcgca cggtgggcga
    10801 ggcgatccgc tcgcccgagg cgcgcgacca cgaactcgtc acccgcatcc cccatccggt
    10861 gctcggctgg gttccgaacg tgcgcttgcc gatccgctac gccgccacgc ccatggccga
    10921 tccggtggcg gccccggcgg tggggcagca tacgcaggag gtcctgcggg acgctcttgg
    10981 cctggacgaa cggcagatcg ccgaactgga gcgcgccggt gccttcggtc ctgcagccgc
    11041 agccgcagcc gcagccgtgc cgggcccgat ggaccgcgac ccggtgcccg catcttgatt
    11101 tcaaccccgc gcgaccggca gcagcggcgc gcattcacaa ccaggagaca accatgaccc
    11161 tgcacacccg tcgcagcgca ctcaggatgc tggccgccgg cggcgccgcc gcttccgtgg
    11221 ggcttcccgc cttcagccag cagggggagg cgtttccctc ccgggtgatc cgcatcgtcg
    11281 tgccgtaccc ggccggcggt accaccgacc agctcgcacg ggcgatcgcc caaccgatgc
    11341 gcgaggtgct gggccagacc gtgatcgtcg aaaacaagcc tggagccggc ggcaccatcg
    11401 gtaccgacta cgtcgccaag cagcccgcgg acggctacac gctggtgttc ggcaacagcg
    11461 gcccgagcgc caccgccggc ctgatgcgca agctgcccta cgacccccag aaggacttcc
    11521 ggccgatctc cggcgtgatg cgggtcccga tgatcctcgc cgtgcccgcc gattcgcccc
    11581 acaagtccgt ggccgaattc gtcgcgtggg cgcggtcgcg gggcgacaag gtgaattacg
    11641 gctccaccgg cgtcggcggc ggttcccacc tctacagcga gtacttcaac gagctggccg
    11701 gcacccgctt ccagcacatc ccttataccg gcgccccgcc gctgctgacc gctttcgccg
    11761 ggggccaggt gcagatggcg ttcgtgacgg ggctggatgg cgctgccatg gtgcaggccg
    11821 gcaaagtccg ctacctggcg gtggcgtcgc ccaagcggac gccagtgctg ccggacctgc
    11881 ctgccatcgc cgaggccgtg cccggattcc aggccgtggg gtggttcggc gtgctggccc
    11941 cggctggcgt gcccgacccc gtcgcacaga agctttccag caccatcgcc gccgcggtgc
    12001 gccgccccga ggtcagcaag ttcttaaccg accgcaacgt ggagccgtgg ggcagcacgc
    12061 aggaagagct gggccgcacg atcgaaggcg agatcgccca gtgggggccg ctcgtgaaga
    12121 agttcaacat cactgtctga tgcggtccca acgatggccc agcgcacact gaagggccgt
    12181 gtcgcgatcg tcggcatcgg ggagacgcac tactacaagc acggccagtc tcccgatgcc
    12241 gagttcaagc tggcgctgca ggccgtgctg cgcgcctgcg ccgacgcggg cctggatcca
    12301 cgccacatcg acggcttcgc ctcgtacggc gacgaccgca gcgaagccac ccgcctggcc
    12361 tccgcgctgg ggctgccgcg cctgcgctcc gccaccatgc agtggggcgg cggcggtggc
    12421 ggctgctgcg ccgcggtcgc caatggcgcg gccgccatcg ccaccggcca agccgactgc
    12481 gtggtggtgt ttcgttcgct caaccagggg cagtacggcc gcttcggcca gagcgccggc
    12541 agcggcacga tctcgggcga gcgcgcctac ctggtgccct acggcgtgat ctcgccgccg
    12601 cagcgcttcg ccatgcggat ccagcgctac atgcatgagc acggcatccg gcaggaggcg
    12661 ctgcgtgcca tcgcactggc gtcctatcac catgcgcagg cgaacccgcg ggccgtgatg
    12721 catggcaagc cgctggacgc ggaaaagtac gatgcctcgc gctggatcgc cgagccgctg
    12781 cacctgttcg actgctgcat ggagaacgac ggcgccgcgg ccatcgtgat ggtggatgcc
    12841 gagcgcgccg ccgatttcgc gcacaagccg gtgtacctgc tcggagccgc ctccgggtcg
    12901 agccatcgct tcgccacgca cgcccacaat tcgccgtcct atgcctcggc gaacttcgaa
    12961 acggtggccg aggatctcta ccggatggcc ggcctcggcc ccgccgacgt cggcgtggtc
    13021 cagagctacg agaacttcac cgggggcgtt gtcatggccc tggccgaaca ccgcttcttc
    13081 agcccgcagg aggcgaacga cttcctcacc ttggagaacc tgatcgcacc gagcgggcgg
    13141 ctgccgctga acaccagcgg cggcaacctc gccgagtgct acatgcatgg cttcgagctg
    13201 gtgctggagg cggtacggca ggtgcgcggc acctccacgg cgcaggcggc gcgcagcgac
    13261 gtggcgctgg tgatcggggg acccatggcg gcgccggtga gcaacctgtt gctgggctcg
    13321 cgcgatgcac tgggagagac accgcgatga ccatgccttc ttcctacctt ccgccgggcc
    13381 tgcccattcc ggttcccgag ccggatggcg tctcggcgcc gtactggaac ggcctgcgcg
    13441 aaggcgtgct gcgcgtgcag cgctgcgcac actgccaggc ctggcagttc gggcctgaat
    13501 ggatctgcca cgcctgcctg caattcgacc cagcctgggt cgaggtggaa ccgtccggcc
    13561 tgatcttcag ctgggagcgc atctggcatc cggcgcatcc ggccctcaag gatcacggtg
    13621 cctacctggc cgtcgtggtg gagcttccgc acgcgggccg cgtccgcatg gtgggcaacc
    13681 tgctgggcga tccgctgcag caagtgcgca tcggggccga agttcagggc gtgttcgagc
    13741 atcacccgga ggcttcgcag ccgtacacgc tgctgcagtg gcgctgccgg tgacggggtc
    13801 gtcttcgacg gatgaaaggc aagccgatat cgacctggtc gtggtcggtg ccggggcggg
    13861 cggcatggtg gccgcgctgg tggcggcgct cgaaggcctg cgcgtcgtgc tgtgcgaggg
    13921 ctcacaacaa gtcggaggca cgacggccac ttccgccggc acgctctgga tcccgggcaa
    13981 ccggcacggg gcggaggcgg gccatgcaga ctcggtccag gcggcggcca gctacctgga
    14041 cgcgctgatc gggccggacg atgggcgcca gctgcggcag gcgttcctgg aatccggcga
    14101 cgcggccatt gcctacctcg aggagcgcac cgacgtcgcc ttcgtcagtg cgggacagca
    14161 tccggactac ctggctttgc ccggtgcggc gatcgctggc cgggcgctgg cgccgcgccc
    14221 cttcgacgga cggctgctgc gtggcgcatt cgcgcggatc cggccgccga tgccggagtt
    14281 cctgctgctc ggcgggatga tggtgggcaa ggccgacatc caggcactgg tgcggcgctg
    14341 gcaatcctgg cccgcgttcg ttgccagtgc cgggctggtg gcccgctacc ttgtcgaccg
    14401 catccgctat ccccgcggca cgcgcctggt gatgggcaat gcgctggtcg cccgcctgtt
    14461 ctacagcctg cagcgcgccg gcgtcgacgt tcgcttcggc tggcgcctgc gggaactgac
    14521 cgtcgaaggc ggcaaggtgc ggggagccac ctttgccgtc gatggccagg agcggcgcct
    14581 cgacagccgg gcgggcgtgg tcctggccac cggtggcgtt ggtcacggcg aggccctgcg
    14641 cgccgaactg gctcctgccg gaatgtcctt gccttcgctg gcttgcgatg cagtcaacgg
    14701 cgaaggcatc accgcggcgc gatccgccgg cgccgggttc gagcgctacg ccgccggcga
    14761 cttcttctgg cagccggtgt cgcgcgtgcc acgcccaggt gggccagggc tttttcccca
    14821 tcttttcctg gaccgcgcca agccgggcct ggttgccgtg gacgcccagg gacgcaggtt
    14881 cgccgacgaa gcgggttcgt accaccactt cgtggaggcg atggtgcgcc acctgcagcg
    14941 caccggcggc gatgcggcct ggctggtctg cgatgcggcc ttcgtgcaca agtacggact
    15001 gggcgtgatc ctgccgggga cgcgccggct ggatcgctgg atccgccaag gctatgtctg
    15061 cgtggccgac cgcctgccgg cgctggcgca gcgcgccggc atcgatcccg cggggctgct
    15121 cgccaccgtg cagcggacca acgactatgc tgcaactggc cgtgacccgg acttccacaa
    15181 gggcgatgcg ccggtcgacc ggttcaacgg cgacccggcc caccgaccga acccttgcct
    15241 gggcgaaatc ggccaaccgc ccttcgtcgc gttgcgcatc ctgccggccg acgcggcttc
    15301 gagttcgggg ctgccgatcg atgccgatgg ccgggtgctg gaccgcgctg gccgtgtcgt
    15361 cgtaggactc tatgcctgcg gcaacgatgc cgcgtccgtg atgcggggca cctatccggg
    15421 cccgggaacg acactggggc cggccttcgt ctttgcctgg cgggccgcgc tgtttgcggc
    15481 gcgtcgtgct gcatcggcgc gtgcgcatcc gatcgaggag aaaccgtgat ggaaccgaat
    15541 gcttcccccg cgcgccgcgc gggcccgctc gcggacttgc gggtcgtcga gttcgcgggc
    15601 atcggaccgg ggcccttcgc ctgcatgctg ctgtcggaca tgggcgccga cgtggtgacg
    15661 atcgaccgcc cgggggcacg cgccagtgat ccgcgcaacg tcacgcagcg gggacgcacg
    15721 gtggtgcagg ccgacctcaa ggacgccgcc tgcatcgagc aggtgctgca gctgctgcag
    15781 gcggccgacg tcctggtgga aggcttccgc ccgggcgtca tggagcgcct ggggctgggg
    15841 ccggaggcgg tcgcgcg
//
LOCUS       DAOOCX010000049        15623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3401_length_15623_cov_9.022610, whole genome shotgun sequence.
ACCESSION   DAOOCX010000049 DAOOCX010000000
VERSION     DAOOCX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15623
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3401_length_15623_cov_9.022610"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..737)
                     /locus_tag="PK399_06365"
     CDS             complement(117..737)
                     /locus_tag="PK399_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12775.1"
                     /translation="MRKAVGNVSTLAVSILLSCLASAGASEQVKVVPEGGLAKDWILQ
                     PGHALAAPGYPATFADRGDDVCVAIGYRVQPDGTTSDYVMLGGWNSSRREKEPANGYW
                     DAYSQAGAAAVSQWRFAPKPGSDMAAVDTVAVMTFRGESSTRTTDELRGKCAVPDLVA
                     RMHVTRRYENGRLEFWKSTNMRGNYQARASVREATQAAGAWAASQR"
     gene            complement(820..1398)
                     /locus_tag="PK399_06370"
     CDS             complement(820..1398)
                     /locus_tag="PK399_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12776.1"
                     /translation="MKKAVLIALALAAGSGAQAQDRVRVVNEGGIRGDWTLAPGTQLS
                     APGYPGAFAKTGDDVCVAVGYRIQPDGSTSDFSLLKTWSSRSGEGEAVEGYWDAFSQA
                     AVAALQQWKFAPRPEVGKPTSVDTVATMTFTGLQSVDAATLRGKCKIEDLAAFLEQVK
                     VDMAKRSDLNRHQIENSYRQQTRSDMVRTPRN"
     gene            complement(1424..2047)
                     /locus_tag="PK399_06375"
     CDS             complement(1424..2047)
                     /locus_tag="PK399_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12777.1"
                     /translation="MSWTRFLLPLPLLLAGAMASATSADGEEARVANEGGIGTWWRLA
                     DGVAIAAPGYPEASLAQRVDACLSVGYTIQPDGSTSDHVIVKRWSSAATPEAVAWNQF
                     GQAASSALSQWRFTPRDNVAPRPTFTVATFTFVSGDAAPSGLAEHCKVADVRDALLAA
                     RQDAYERGDINQKWLDRLYRETMRQEILNNAANLCSRSQSTATSCVF"
     gene            complement(2119..3612)
                     /gene="pyk"
                     /locus_tag="PK399_06380"
     CDS             complement(2119..3612)
                     /gene="pyk"
                     /locus_tag="PK399_06380"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPW12778.1"
                     /translation="MTARKTEQAAVPHQRRTRIVATLGPATDPPEMLEAVLRAGVDVV
                     RLNFSHGDPSAQVARGEAVRAMALKIGREIGILADLPGPKIRVETFAEGKVALKAGNR
                     FDLVASLDAPPGDATQVGVSYLELPGDVVAGDVLLLDDGVVQLQVTAVEGERIVTTVL
                     NDCFLSNRKGLNKQGGGLSLGAITEKDRELIAIAAGMNVDFIAVSFCRNADDMNEARR
                     VAKAHGSSAALVAKIERAEAIENLTEIVDASDVVMVARGDLGVEIGDAELPGLQKKII
                     REAVAREKVVITATQMLQSMVDSPIPTRAEVLDVANAVIDGTDAVMLSQESAAGSWPV
                     KAVEAMARICVGAEKQFVADTDFEAAQRGLERADQAIAMATMFLSEHIGVRGVVAMTE
                     SGGTARFLSRFRSSVPIFAFSRHGPARRKMALMRDVFPMDYDSRGQTPREAARGSIRL
                     LVDAGLLQPGDRVVFTSGEHMETRGATNTLRLLEIGEDGRAAGLGEL"
     gene            complement(3698..5950)
                     /locus_tag="PK399_06385"
     CDS             complement(3698..5950)
                     /locus_tag="PK399_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW12779.1"
                     /translation="MRIRRRAALALAVALAAGPGLAQSPAKVDAAGLWDAFLAKATPD
                     DVNTALDALAAVDYGLDSVDAGKCRDKAADLARAQVLAPVSIAVQRAALLCAEATGDH
                     PAAERATATIAALARQAFADADRGAWPRPVRIVSPADAYALFASAGLEFRYEMYPQLR
                     PAPYFPLVIAAASPTTGVERVVRFDYVDVLQAIDRKDPAHGTPRLRMAYSESFVDSAA
                     KREDIAAVDLLAVQEAIAEDAPAKRVDALRAAVQAGGLHAATTWLAVCVNTPSRGCGD
                     GLVDALLPLAEARHAYPMMLLANAYLEGVGVPRDQASAEAMLDAADRLWEQRGASVAF
                     AEVQSLLHPGQPLAPFLRKRLDAAHAAGHPAARVVAVAMDMGREDGKYVLSPADEALL
                     ADPAHNGTGQGLFKLAAWHEDRDKAVSDAYLKRAAEAGNASALRIVSLRLREAEGNRP
                     AAPTTIAWLEKAANGGDPVAMRYLAWRALSDGGPRRAEDWLMHAAAQGDVDALFFLAS
                     LWAGGYKDMSGDAARAKDLYESLALVDGQGPRARRMLASMAIEGNGMSKDPARAKAWL
                     LQDAEAGDVESQAELGGRLLGSSLGASDEAAGRKWLERAIASGSIDAMNEYGLWLHNA
                     GKDAADRARGVQLARQAVEKDDAGALNNLAWMLCTSTHDDVRKPADGMTYVARLEKMP
                     DLTPGTVDTLAACYAAVGQYPRAVELQQQVIADMRKRPAPALANIKEMEARLALYRAG
                     KPYIEVPAKR"
     gene            complement(5950..7980)
                     /locus_tag="PK399_06390"
     CDS             complement(5950..7980)
                     /locus_tag="PK399_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPW12780.1"
                     /translation="MLRWMAMVLAFGLAMGSARADEFASGGFSFRTGEVPAFVEDRPL
                     PSEWPAAAPGAQDEQWRFWRYDRQVDHRPGADVAYTDYVYEPRSQGNIADAGRFDLSF
                     NPDYQQLTLHRVELRRNGRWESRLKPKDISIARRERQFEQDIADGLVAALIVIEDVRA
                     GDVVRISWSVTGSNPILAGQFSEQARLAFSHPILDLRLRAYYAPGTQIASHLENGAPA
                     PTVSTSADATVVEVRAAAVDRLQDEGDYPVWYQPYPLVQFAPRRSWADVVAWALPLYP
                     DQSGVALPADLEQRIAQWKKIGPASERLQAALRAVQDEVRYFGVETGTSSHRPRMPNE
                     VWTRRFGDCKDKTWLLVTVLQRLGIRAMPALVNTDRGRGTQGYAPSADVFDHVIVRAE
                     LDDRAVYVDPTMRLQGGAPERGDLSGYGFVLPVAAGQAALAEVLAPVDALEGVKTHEH
                     YAVDGDGLRLEVTSEFAGMSADQTRRALDEERIEDRGRRYTEYYGKRYGAIQSLQPPV
                     VEDDRARNVIRVRETYRLGAPWQREGDTRSLDLEAEALSAVSALPDRTDRRGPLYFAR
                     PGRYVQEVSVEAPAGWVPRFAREDERIDSKAFGYKRTLEPTETGARLRHELDVRQREV
                     EVAQVQAHVSDLRKLREGSSSRLRYRAPASAEAADREARLRSLLQDAMEGKQ"
     gene            complement(8005..9186)
                     /locus_tag="PK399_06395"
     CDS             complement(8005..9186)
                     /locus_tag="PK399_06395"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012445624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPW12781.1"
                     /translation="MSIVRMTDLDLTGKRVLIRQDLNVPVADGRVTSDQRITASIPTL
                     RLALEKGAAVMVTSHLGRPKEGTWSEADSLAPVAARLSELLGFEVPLVRDYLDGVDVQ
                     PGQLVLLENCRMNIGEGKDDEALSKRYAALCDVFVMDAFGTAHRAQASTHGAIRFAKQ
                     AAGGPLLMAELDALDKALASPARPLLAIVAGSKVSTKLELLSSLVGKVDQLIVGGGIA
                     NTFIAALGHGVGKSLVEMDLIDTAKQIMADAKARGADIPLPLDVVVAPAFAADAPATI
                     KRIDEVGADDMILDIGPRTAQAYAALIAKAGTVVWNGPVGVFEFDAFGHGTEAVARAI
                     AASPAFSIAGGGDTLAAVDKYAIENDVSYISTGGGAFLEFLEGKTLPAVAALEARGVV
                     A"
     gene            complement(9251..10609)
                     /locus_tag="PK399_06400"
     CDS             complement(9251..10609)
                     /locus_tag="PK399_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HPW12782.1"
                     /translation="MKKLAWWSLLPLVAFAATRDDYASQWPLALQREDSGAYRVVLDR
                     EVYRQLQSPRLRDLVVVNAQGTPVATAVFPADAPLAQSGTTVALPWFPLPLDAGASRQ
                     DIAAISEIATDGSLRRVEMRAPGDATAATGDGGFVVDASQLRVPLAALRIAWSDAAAF
                     DRGYRVSASDDLRQWREVDADARLVQLQNNGRRIVEDRIALPAVQARYLRLLPGSTQA
                     SALIVTGVIAEPVGQIAEPALQWEALQGKRVDTPQGATFEYTLDGRFPIEAADVVSTG
                     NSTRGWTLERRDDPQSAWQPAAPPWMAYRLDSAGTSSRSPPQPLHVGSRDHHWRLRAR
                     DAFDGAPPLLRLGYRPEVVVFLAEGAPPFALLAGSARASRQEAPLPQLVDALRAERGR
                     DWQPATASLGKRELLAGDAALQPLEPPTDWKRWLLWGVLVVGAALVAGFAVSLLRGKP
                     AG"
     gene            complement(10606..13326)
                     /locus_tag="PK399_06405"
     CDS             complement(10606..13326)
                     /locus_tag="PK399_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HPW12783.1"
                     /translation="MEGLIALLVLAVLAVPVLLVVALASISGLKQRVGALEQQLAALQ
                     RRPVAPAASAAAPEPTLAELVRQPAPPPSHPAPQVAPPPVQDAPVAPPARAAEPPAPQ
                     VGEPMASMPSPASVPRPIPRPQPAKPATPGIATIAMRAVQRWFTVGNVPVKIGMLVLL
                     AGVASLLKYGADQGWFSMPPELKLAGVAAAALAALVFAWRKRASNRVFALSLQGGAIG
                     VLLLVVFAAFKNFGMLPAGAAFALSIVLVASAGVLAVLQDARALAVFALLAGFLAPIW
                     LSTGSGNHVALFSYYAVLNAAIVGIAWHKAWRILNLLGFVFTFGIGIAWGALDYSQAK
                     YASTQPFLVLFFAMYLLIPILFARRRPVGRRDLIDGCLVFGMPLVAFSLQAGLLDGKR
                     MALAFCALGLGALYAGLSWWLRRRKGFDVLAQSHALLAVGFATLAVPLALSAQATACV
                     FALEGAALCWLGLRQQRLLPQLAGAGLQLAAAVAFTIGLTAARFSPAAEASAPIANAA
                     FMGALLVAIAGFASAWAYRQARNDGIAGLYYGWGLAWWVGNGLNEIVRFLGHRDEPDA
                     LLAFAALSGWLAGEVHRRRPAGALASTGLAALASAVPLALWQSGVHEQPFAGNGAWAW
                     VVYALLGIRSLQCLRDGEHRLASPAQFTWWLVWPLVASLLLGWLPSRFDGMGDGWRAA
                     LIASPWLAVAALALYRWRWLSMPLGEAFDGWRGRLLAVLFAVLGLWWLVSLFDAGAAS
                     PLPWLPVLNPMELVQLAVLVLALRWQLPAGGRLSSSRIVLLSGLAFAWITSVVLHAVH
                     HWGGVAWNDALLSSSLAQTSLTVVWSVLGVIGWVLGSRRGQRMLWLAGAVLMGVVLAK
                     LVLVDRQHLGNLLGIGSFIAYGLLCTVVGYLAPAPPRSADADLDAKETAA"
     gene            complement(13421..15100)
                     /locus_tag="PK399_06410"
     CDS             complement(13421..15100)
                     /locus_tag="PK399_06410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPW12784.1"
                     /translation="MTPDAPIAGWSLRRELTRMLVLTALLPALLFGMALLWSQWQRDQ
                     DDLKLRLGSNAQLSANAIDDFLQGQLSGVELMADGSMRSGRTLDEELGKLLRAYPAML
                     RALATDREGNIVAARSKNGRPAALAGERVADREWFLAVRAGGRPHVSDADRARLYGSE
                     PLVAVSAPMRRDGAFDGVLQASIPIEQYIRVRSDNLRRRGFELLLLDRQDRVVHASKA
                     LRWQFLDATGTVGALIRRGAARPGQPGQMRTRGGLLRNGEDAYVHAVVMRTGWVVAII
                     APEQRMLAPALPRVGLLFGLLAVTTLGVLFALWRLRRLLAASMGRLLASLHGYALGGS
                     LDPAQLTRMPEELQPLAGGIGDLAARMNAAFGELQQVLDQREHIIEERTESLRLAVSD
                     LDRLSRTDALTGSLNYRGFQEAGERLWKEARATGAELSVLALDIDHFKKYNDLYGHAE
                     GDGALRRFAGAVSSALLHADDLLARPGGEEFVVFLPASTQEQAMRVGQRVCERVRSAD
                     IVHAASPEGRMTVSIGVASVQPGDDDVEQMLRRADAALYRAKAAGRNRVSD"
     gene            complement(15197..>15623)
                     /locus_tag="PK399_06415"
     CDS             complement(15197..>15623)
                     /locus_tag="PK399_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPW12785.1"
                     /translation="NKDWRGGRGILENIIPSSTGAAKAVGLVLPQLKGKLTGMSFRVP
                     TSDVSVVDLTVELENPATYAEICAEMKAQSEGALKGILGYTDEKVVATDFRGDARTSI
                     FDAEAGIALDDTFVKLVSWYDNEWGYSNKCLEMVKVVAA"
BASE COUNT         2511 a         5650 c         5149 g         2313 t
ORIGIN      
        1 cgggcggttt cggcgagttg ttcgatgctc atgggcggcg tccggcggcg tttgtgagcc
       61 cacgattata gccgccaccg ggcgcccccg aaggggacgc ccgtggtttc cgcgactcag
      121 cgctgggagg cagcccacgc tcctgcagct tgcgtggcct cgcggacgct tgcccgcgcc
      181 tgatagttcc ccctcatgtt ggtgctcttc cagaactcca ggcgcccgtt ctcgtagcga
      241 cgagtgacgt gcatgcgcgc gaccaggtcc ggcaccgcgc actttccacg caattcatca
      301 gtggtgcggg tggacgattc gccacggaac gtcatcaccg cgaccgtgtc caccgcggcc
      361 atgtcgctgc ccggcttcgg tgcgaagcgc cactgcgaga ccgccgcggc gcccgcctgc
      421 gagtaggcat cccagtagcc gttggcgggc tccttttcgc gcctgctgct gttccagccg
      481 cccagcatca cgtagtcgga ggtggtgccg tccggctgca cgcgataccc gatcgcgacg
      541 caaacatcgt caccacgatc ggcgaaggtg gccgggtaac caggcgcggc cagtgcatgg
      601 ccaggctgga ggatccagtc cttggccagg ccgccttccg gcaccacctt gacctgctcc
      661 gacgcgcctg ccgaggcaag gcacgacagc aggatcgata ccgccaaggt gctcacattt
      721 cccactgcct tcctcatcgc ccgactcctt ccgttggttg tcgataccgg ttccgatcat
      781 gtctcgcccc gcgccccgac cgggacgcag gaccttgcgt cagttgcgcg gcgtacggac
      841 catgtcgctg cgcgtctgct ggcggtagct gttttcgatc tggtgccggt tcaggtcgct
      901 gcgcttggcc atgtccacct tcacctgctc caggaaggcg gccaggtctt cgatcttgca
      961 tttgccacgc agggtggccg catcgacgga ctgcaggccg gtaaaggtca tcgtcgccac
     1021 cgtgtccacc gaggtgggct tgccgacctc gggccgcggg gcgaacttcc attgctggag
     1081 ggccgcgacg gcggcctgcg aaaacgcgtc ccagtagccc tcgaccgcct cgccttcgcc
     1141 gctgcggctg gaccaggtct tcaacagcga gaagtcgctg gtgctgccat ccggctggat
     1201 cctgtagccc acggccacgc acacgtcatc gcccgtcttc gcgaacgcac ccggatagcc
     1261 gggcgcggac agctgggtgc ccggcgcgag cgtccagtcg ccgcggatgc cgccttcgtt
     1321 gacgacccgc accctgtcct gcgcttgcgc gccggagcca gccgcaagtg ccagcgcgat
     1381 caaaacagcc ttcttcatcg acatatcgtt cgttcctcgt tgactagaac acgcaactcg
     1441 tagccgtgct ctgactgcga ctgcacaggt ttgcggcatt gttcaggatc tcctggcgca
     1501 tcgtttcacg atacaggcga tccagccatt tctgattgat atcgcctcgt tcatacgcgt
     1561 cctggcgcgc ggcgagcagg gcgtcgcgca cgtcggcgac cttgcaatgt tcagccagcc
     1621 cggacggcgc ggcatcgcca ctgacaaacg tgaatgtcgc cacggtgaag gtcggccttg
     1681 gcgcgacgtt gtcgcgagga gtgaagcgcc attgcgagag tgcgctggat gcggcttggc
     1741 cgaactggtt ccacgccacc gcctcgggtg tcgccgcact gctccagcgc ttcacgatga
     1801 cgtggtcgga cgtactgcca tcgggctgga tcgtatagcc caccgagagg caggcatcga
     1861 cgcgttgggc cagcgacgct tccggatacc cgggcgccgc gatcgcgacc ccgtccgcca
     1921 atcgccacca cgtgccgatg ccgccttcat tggcgacacg cgcttcctcg ccatcggccg
     1981 atgtcgccga tgccatcgca ccggcgagca gcaagggaag cggcagcagg aagcgagtcc
     2041 aggacatcgc gcaacctcgg gcatcgaccg gaacctcaat gaacacccga ccggatcagg
     2101 ctgtcaagcg agccccgttc agagttcgcc caatcccgcc gcgcgtccgt cctcgccgat
     2161 ttccagcagg cgcagcgtat tcgtggcgcc gcgggtttcc atgtgctcgc cgctggtgaa
     2221 caccacgcgg tcgccgggct gcagcaggcc ggcgtcgacc agcaggcgga tgctgccgcg
     2281 cgcggcctcg cgcggagtct gcccgcgact gtcgtagtcc atcgggaaca cgtcgcgcat
     2341 cagtgccatc ttgcggcgcg ccggcccgtg gcgggagaac gcgaagatcg gcaccgacga
     2401 gcggaagcgc gagaggaagc gcgcggtgcc gccggactcg gtcatcgcca ccacgccgcg
     2461 cacgccgatg tgttcggaca ggaacatggt cgccatcgcg atggcctggt cggcccgctc
     2521 caggccgcgc tgcgcggcct cgaaatcggt gtcggcaacg aactgcttct cggcacccac
     2581 gcagatccgc gccatcgcct ccactgcctt caccggccag ctgccagcgg cactttcctg
     2641 cgagagcatc accgcgtcgg tgccgtcgat cacggcattc gcgacgtcga ggacttcggc
     2701 gcgggtcggg atcgggctgt cgaccatcga ctgcagcatc tgcgtcgcgg tgatcacgac
     2761 cttctcgcgc gcgaccgcct cgcggatgat cttcttctgc aggcccggca attcggcatc
     2821 gccgatctcc acgccgaggt cgccacgcgc gaccatgacc acgtcgctgg cgtcgacgat
     2881 ctcggtgagg ttctcgatcg cctcggcacg ctcgatcttg gcgaccagtg cggcactgct
     2941 gccgtgcgcc ttcgccaccc ggcgcgcttc attcatgtcg tccgcattgc ggcagaacga
     3001 caccgcgatg aagtccacgt tcatgcccgc ggcgatcgcg atcagctcgc ggtccttctc
     3061 ggtgatcgcg cccagcgaca gaccgccgcc ctgcttgttg agccccttgc gattcgacag
     3121 gaagcaatcg ttgagcacgg tggtgacgat gcgctcgccc tccaccgcgg tgacttgcag
     3181 ttgcacgacg ccgtcgtcga gcaagagcac gtcgccggcg accacgtcgc ccggcaactc
     3241 gaggtagctc acgccgacct gggtcgcatc ccccggcggt gcatcgaggc tggcgaccag
     3301 gtcgaagcga ttgcccgcct tcagcgcgac cttgccctcg gcgaaggtct cgacgcggat
     3361 cttcgggccc ggcaggtcgg cgaggatgcc gatctcgcgg ccgatcttca gcgccatcgc
     3421 acgcacggct tccccacgcg ccacctgcgc cgagggatcg ccgtgggaga agttcaaccg
     3481 caccacgtcc acgcccgcgc gcaacacggc ttccagcatc tcgggcggat ccgtggccgg
     3541 ccccagggtg gcgacgatgc gggtgcggcg ctggtgcggg acagcggcct gttcggtctt
     3601 gcgtgcggtc atgtgtgcgg gctcctgtgg cggcgaacgc tagcacagca ccgcgaccgg
     3661 cgatggctcg ccggacaatg ccgatggcgt cctcggctca gcgcttcgcg ggtacttcga
     3721 tatacggctt gcccgcacga tagagcgcca gccgcgcttc catttccttg atgttggcga
     3781 gggccggggc cggacgcttg cgcatgtccg cgatcacctg ctgctgcagt tccaccgcgc
     3841 gcggatactg ccccaccgct gcgtaacagg cggccagcgt atcgaccgtg ccgggggtga
     3901 ggtcgggcat cttttccagc ctggcgacat acgtcatgcc atcggccggc ttgcgcacat
     3961 cgtcatgggt ggaggtgcac agcatccatg cgaggttgtt cagggcaccg gcatcgtcct
     4021 tttccaccgc ctgcctggcc aactgcacgc cgcgcgcgcg gtctgccgca tccttgcctg
     4081 cgttgtgcag ccacagcccg tactcgttca tcgcatcgat cgaaccggat gcgatcgccc
     4141 gctccaacca cttgcggccg gcggcctcgt cgctggcacc caggctggag cccagcagtc
     4201 gcccgcccag ctcggcctgc gactccacgt cgcccgcttc ggcatcctgc aacaaccatg
     4261 ccttggcacg cgcaggatcc ttcgacatgc cgttgccttc gatcgccatc gaggccagca
     4321 tccggcgcgc gcgtggtccc tggccgtcga cgagggccag cgattcgtac aggtccttcg
     4381 cccgcgccgc atcgccactc atgtccttgt agccgcctgc ccacaggctg gcgaggaaga
     4441 acagcgcatc cacatcgccc tgcgcggcgg cgtgcatcaa ccagtcctcc gcacggcgcg
     4501 ggccgccatc cgacagcgca cgccaggcca ggtagcgcat ggccaccgga tcgccgccgt
     4561 tcgcggcttt ctccagccag gcgatggtgg tgggcgctgc gggacgattg ccctcggcct
     4621 cgcgcaggcg cagggacacg atgcgcaacg cggacgcgtt gccggcctcc gcggctcgct
     4681 tcaggtaagc gtccgacacg gccttgtcgc gatcctcatg ccaggcagcg agcttgaaca
     4741 ggccctggcc ggtgccgttg tgggcgggat ccgcgagcag cgcctcatcg gccggggaca
     4801 ggacgtactt gccatcctcg cgtcccatgt ccattgcgac cgcgacgacc ctggccgccg
     4861 ggtggccggc cgcgtgcgcg gcatcgaggc gcttgcgcag gaacggcgcc agcggttgcc
     4921 cgggatgcag caacgactgc acttcggcaa aggcgaccga ggccccgcgc tgttcccaca
     4981 ggcgatcggc ggcgtcgagc atggcctctg ccgaggcctg gtcgcgcggc acgccgacgc
     5041 cctccaggta ggcgttggcg agcagcatca tcgggtaggc atggcgggct tctgcgagcg
     5101 gcagcagcgc atccaccagg ccgtcgccac agcccctcga tggtgtgttg acgcacaccg
     5161 ccagccaggt ggtggccgca tgcaggcccc cggcctgcac cgcggcgcgc agggcatcca
     5221 cgcgcttggc cggtgcgtct tcggcgatcg cttcctgcac cgccagcagg tcgaccgcgg
     5281 cgatgtcctc gcgcttggcc gcgctgtcca cgaacgactc ggagtaggcc atccgcaaac
     5341 gcggggtgcc atgggcggga tccttgcggt cgatcgcctg caacacgtcg acatagtcga
     5401 accgcacgac gcgctccaca ccggtggtcg gcgatgccgc ggcgatcacc agcgggaaat
     5461 acggcgcggg acgcagttgc gggtacatct cgtagcggaa ttccaacccg gccgaggcga
     5521 acagcgcata ggcatccgcg ggcgacacga tgcgcacggg acgcggccat gcgccacgat
     5581 ccgcgtcggc gaatgcctgc cgggccaggg ccgcgatggt ggcggtggcg cgttcggccg
     5641 cggggtggtc gccggtcgcc tcggcgcaca gcagggcagc gcgctgcacc gcgatcgaga
     5701 ccggcgccag cacctgcgca cgcgccaggt cggccgcctt gtcgcgacac ttgccggcat
     5761 cgaccgaatc cagcccgtaa tccactgcag ccaatgcatc cagtgcggta ttgacgtcat
     5821 ccggcgtggc cttggccagg aacgcgtccc acaggccggc ggcatcgacc ttcgccgggg
     5881 actgggcaag gcctgggccc gcggccagcg caacagcgag ggccagggcg gcccggcggc
     5941 gaatcctcat cattgttttc cttccatggc gtcctgcagc agcgagcgca ggcgcgcctc
     6001 gcggtcggcg gcttcggcgc tggccggcgc gcggtagcgc aagcgcgaag aactgccctc
     6061 gcgcaacttg cgcaggtcgc tgacgtgggc ctgcacctgc gcgacctcga cctcgcgctg
     6121 gcggacgtcc agttcatggc gcaggcgcgc gccggtttcg gtgggctcga gcgtgcgctt
     6181 gtagccgaac gccttcgagt cgatgcgctc gtcctcgcgg gcgaagcgcg gcacccagcc
     6241 ggccggcgct tccacgctga cttcctgcac gtaacgcccc ggacgcgcga agtacagcgg
     6301 accgcgacga tcggtgcggt cgggcaaggc actgaccgcg gacagcgctt ccgcctccag
     6361 gtccaggctg cgtgtgtcgc cctcccgttg ccagggtgca ccgagccgat aggtttcgcg
     6421 gacgcggatc acgttgcgcg cgcggtcgtc ctcgaccaca ggcggctgca gcgactggat
     6481 cgcgccgtag cgcttgccgt agtactcggt gtaacggcga ccgcgatcct cgatgcgttc
     6541 ctcgtcgagc gcgcgccggg tctggtccgc actcatgccg gcgaactcgc tggtgacctc
     6601 caggcgcagg ccatcgccat cgaccgcgta gtgctcgtgg gtcttcaccc cttccaacgc
     6661 atcgacgggc gccagcacct cggcgagtgc cgcttggccg gcagccaccg gcagcacgaa
     6721 cccgtagccg gacaggtcgc cacgctccgg cgcaccgccc tgcaggcgca tggtcggatc
     6781 gacgtacacg gcacggtcat cgagttcagc gcgcacgatc acgtggtcga agacgtcggc
     6841 cgaaggcgca tagccctggg tgccgcggcc gcggtcggta ttcaccagcg ccggcatggc
     6901 gcggatgccg aggcgctgca gcaccgtcac cagcagccac gtcttgtcct tgcaatcgcc
     6961 gaagcgccgc gtccacacct cgttcggcat gcgcgggcga tgcgaactgg taccggtctc
     7021 cacgccgaag tagcggacct cgtcctgtac cgcgcgcagc gccgcctgca ggcgttcgct
     7081 ggcgggtccg atcttcttcc actgcgcgat ccgctgctcc aggtcggccg gcaacgcgac
     7141 cccggactgg tccggataca gcggcaaggc ccacgccacc acgtccgccc agctgcggcg
     7201 cggcgcgaac tggaccaggg gatacggctg gtaccagacc gggtagtcgc cctcgtcctg
     7261 caggcgatcg accgcggccg cacggacttc gaccacggtc gcatcggccg acgtcgagac
     7321 ggtgggcgcg ggcgctccgt tttccaggtg cgatgcgatc tgcgtgcccg gcgcatagta
     7381 ggcgcgcagg cgcaggtcga ggatcgggtg cgagaacgcc agccgggcct gttcggagaa
     7441 ctggcccgcc aggatcgggt tgctgccggt cacgctccag ctgatccgaa ccacgtcgcc
     7501 ggcacgcacg tcctcgatca cgatcagcgc cgccaccagg ccatcggcga tgtcctgctc
     7561 gaattggcgc tcgcggcggg cgatcgagat gtccttcggc ttgagccgcg attcccaacg
     7621 gccattgcgg cgcaattcga cccgatgcag ggtcagttgc tggtagtccg ggttgaatga
     7681 caggtcgaaa cggccggcgt cggcgatgtt gccctgcgag cgcggttcat agacgtagtc
     7741 ggtgtaggcg acatcggcgc cgggacggtg gtcgacctgg cggtcgtagc gccagaaccg
     7801 ccactgctcg tcttgcgcgc ctggtgcggc agccggccat tcggacggca acgggcggtc
     7861 ctcgacgaac gccggcacct ccccggtgcg gaacgagaac ccaccgctcg cgaactcatc
     7921 ggcccgggcc gaccccatgg ccagcccgaa ggccagcacc atcgccatcc atcgcaacat
     7981 gcgcaactcc atttgactgc cgtcctacgc taccaccccg cgcgcctcca gcgccgcgac
     8041 cgccggcaag gttttgcctt cgaggaattc gaggaacgca ccgccgccgg tggaaatgta
     8101 cgagacgtca ttctcgatcg cgtacttgtc gaccgcggcc agggtgtcgc caccgccggc
     8161 gatcgagaat gccggcgagg ccgcgatcgc gcgcgccacc gcttcggtgc cgtggccgaa
     8221 ggcgtcgaac tcgaacacgc cgaccgggcc gttccagacc acggtgccgg ccttcgcgat
     8281 gagtgccgcg taggcctgcg cggtccgtgg gccgatgtcg aggatcatgt cgtcggcgcc
     8341 gacttcgtcg atgcgcttga tcgtggccgg cgcatccgcg gcgaatgcag gcgcgaccac
     8401 cacgtcgagc ggcaacggga tgtccgcgcc gcgcgccttg gcatcggcca tgatctgctt
     8461 cgccgtatcg atcaggtcca tctccaccag cgacttgccg acgccatggc cgagcgcggc
     8521 gatgaaggtg ttggcgatgc cgccgccaac gatcaactgg tccaccttgc cgaccagcga
     8581 ggacagcagt tccagcttgg tcgaaacttt cgatccagcg acgatagcga gcagcggtcg
     8641 cgccgggctg gccagcgcct tgtcgagtgc atcgagctcc gccatcagca acggtccgcc
     8701 cgcggcctgc ttcgcgaagc ggatcgcgcc gtgcgtcgag gcctgcgcgc ggtgcgcggt
     8761 gccgaaggca tccatgacga acacgtcgca gagtgcggcg taccgcttcg acaaggcctc
     8821 gtcgtccttg ccctcgccga tgttcatccg gcagttttcc agcagcacca gctggccggg
     8881 ctgcacgtcc acgccatcca ggtaatcgcg caccagcggc acctcgaaac cgagcagttc
     8941 cgacagccgc gccgcgaccg gcgccagcga atcggcctcg ctccacgtgc cttccttcgg
     9001 acggccgaga tgcgaggtga ccatcaccgc cgcgcctttc tccagcgcca gcctgagggt
     9061 cgggatggac gcggtgatgc gctggtcgga ggtcaccctg ccatcggcaa cgggcacgtt
     9121 gaggtcctgg cggatcagca cgcgcttgcc ggtcaggtcg aggtcggtca tgcggacgat
     9181 ggacatggca ttcctgcggg ggacgaaggc ctccattgtc ccggccgcgc gcggtcgccg
     9241 caaacggcgt tcaacccgcc ggcttgcccc gcagcaggct gacggcgaag ccggccacca
     9301 gcgcggctcc gaccaccagc acgccccaca gcagccagcg cttccagtcg gtgggtggct
     9361 caagcggctg caacgcggca tcgccagcga gcaactcgcg cttgccgagg ctggcggtgg
     9421 cgggctgcca gtcgcgaccg cgctcggcac gcagcgcatc caccagctgc ggcaagggcg
     9481 cctcctggcg agatgcccgt gcgctgccgg cgagcaacgc gaacggcgga gcgccttcgg
     9541 cgaggaacac cacgacttcc ggccggtagc ccaggcgcaa caacggcggc gcgccatcga
     9601 aggcatcgcg cgcgcgcagg cgccaatggt gatcgcgcga gcccacgtgc agcggctgcg
     9661 gcggcgaccg gctggaggtg cccgcgctgt ccaggcgata cgccatccac ggcggcgccg
     9721 caggttgcca tgcggactgc gggtcgtcgc gccgctccag ggtccagccg cgcgtgctgt
     9781 tgccggtcga caccacgtcc gctgcttcga tgggaaaacg accgtcgagc gtgtactcga
     9841 aggtcgcgcc ttgcggcgtg tcgacgcgct tgccctgcaa tgcctcccac tgcaacgccg
     9901 gttcggcgat ttgcccgacg ggctccgcga tgacgccggt gacgatcaat gcactggcct
     9961 gcgtcgaacc cggcaacagg cgcaaatacc gcgcctgcac cgccggcaac gcgatgcgat
    10021 cctcgacgat gcgccggccg ttgttctgca gctgcacgag gcgcgcgtcg gcatcgacct
    10081 cgcgccactg gcgcaggtcg tcggaggcgc tgacgcgata gccgcgatcg aacgcggcgg
    10141 catccgacca cgcgatgcgc aacgccgcca atggaacgcg cagctggctg gcatcgacca
    10201 cgaaaccgcc atcgcccgtc gcggccgtcg catcgccggg tgcacgcatt tcgacccggc
    10261 gcaggctgcc gtcggtggcg atctcgctga tcgccgcgat gtcctggcgg gatgctccgg
    10321 cgtcgagcgg caacgggaac cacggcaagg cgaccgtcgt gccggattgc gccagtggtg
    10381 catcggcggg aaacaccgcg gtcgccaccg gcgtcccctg cgcattgacc acgaccaggt
    10441 cccgcagccg gggcgattgc agctggcggt aaacctcgcg atccagcacg acgcgatacg
    10501 caccgctgtc ctcgcgttgc agggcaagcg gccactggct ggcgtagtcg tcgcgtgtgg
    10561 cggcgaacgc caccaacggc agcaacgacc accatgcgag cttcttcatg ccgcggtctc
    10621 ctttgcgtcg aggtcggcgt cggcactgcg cggcggggcc ggcgcgaggt agccaaccac
    10681 cgtgcacaac aggccgtagg cgatgaacga accgatgccg agcaggttgc ccaggtgctg
    10741 ccgatcgacc agcaccagct tggccagcac cacgcccatc agcaccgcgc cggccagcca
    10801 caacatgcgc tggccacgac gcgaacccag cacccagcca atcacgccca gcacgctcca
    10861 caccacggtc aggctggtct gcgccaggct ggacgagagc aatgcatcgt tccaggccac
    10921 cccgccccag tgatgcacgg catgcagcac caccgaggtg atccaggcga aggccaaccc
    10981 ggacagcagc acgatgcgcg acgacgacaa gcgaccgccc gccggcagct gccagcgcag
    11041 cgccagcacc agcacggcga gttgcacgag ctccatcggg ttcagcacgg gcaaccacgg
    11101 cagcggcgat gctgcgccgg catcgaacag cgagaccagc caccacaggc ccagcacggc
    11161 gaacaacacg gccagcaggc gaccacgcca gccatcgaag gcctcgccga gcggcatcga
    11221 caaccagcgc cagcgataca acgccagcgc cgccaccgcg agccagggcg atgcgatcaa
    11281 cgcagcccgc cagccgtcgc ccatgccgtc gaagcggctc ggcaaccagc ccagcagcag
    11341 gctcgccacc agcggccata ccagccacca ggtgaattgc gccggcgatg ccaaccggtg
    11401 ttcgccatcg cgcaggcatt gcaggctgcg gatgccgagc agcgcataga cgacccatgc
    11461 ccacgcgcca ttgccggcga agggctgctc atggacaccg ctctgccaca gcgcgagcgg
    11521 cacggccgac gccagcgctg ccagtccggt cgatgcaagt gcaccggcag gacggcggcg
    11581 atgcacctcc cccgccagcc aaccgctgag cgcggcgaag gcgagcagcg cgtcgggctc
    11641 atcgcgatgg cccaggaaac gcacgatttc attgaggcca ttgccgaccc accaagccag
    11701 tccccatccg tagtagaggc cggcgatgcc gtcgttcctg gcctggcgat acgcccacgc
    11761 gctggcgaaa cccgcgatcg caaccagcag ggcgcccatg aacgcggcat tggcgatcgg
    11821 cgccgaggcc tccgcggccg gcgaaaaacg cgccgcggtc aacccgatgg tgaacgccac
    11881 cgcagcagcc agctgcaggc ccgcaccggc gagctgtggc aacaaccgct gctggcgcaa
    11941 tccgagccag cacagtgccg cgccttccag cgcgaacacg caggcggtcg cctgcgccga
    12001 caacgccagc ggcaccgcca gcgtggcgaa gccgaccgcg agcaaggcat gcgactgcgc
    12061 cagcacgtca aagcccttgc gccggcgcaa ccaccacgac agcccggcat acaacgcgcc
    12121 gaggccgagc gcgcagaacg ccagcgccat gcgctttccg tccagcaagc cggcctgcaa
    12181 ggagaacgcg accagcggca tgccgaacac caggcagcca tcgatcaggt cgcgccggcc
    12241 gaccgggcga cgccgcgcga acaggatcgg gatcagcagg tacatcgcga agaacaggac
    12301 caggaacggc tgggtgctgg cgtacttcgc ctgcgaataa tccagtgcgc cccaggcgat
    12361 gccgatgccg aaggtgaaca cgaaacccag caggttgagg atccgccagg ccttgtgcca
    12421 ggcgatgccg acgatcgccg cgttcaatac cgcgtagtac gagaacagcg ccacatggtt
    12481 gccgctgccg gtggacagcc agatcggcgc caggaagccg gccagcaagg cgaataccgc
    12541 cagtgccctg gcgtcctgca gcaccgccag cacgcctgcg cttgcgacca ggacgatgct
    12601 caacgcgaac gccgcgcccg cgggcagcat cccgaaattc ttgaacgcgg cgaacaccac
    12661 cagcagcagc acgccgatcg cgccgccctg caggctcagc gcgaacacgc ggttgctcgc
    12721 gcgcttgcgc caggcgaaca ccagtgcggc gagcgcggcg gcagccaccc cggccagctt
    12781 caactccggc ggcatcgaga accagccctg gtcggcaccg tatttcagca acgacgccac
    12841 gcccgcgagc aggaccagca tgccgatctt caccggcacg ttgcccacgg tgaaccagcg
    12901 ttgcaccgcg cgcatggcga tcgtcgcgat gcccggcgtg gcgggcttcg cgggctgcgg
    12961 gcgcgggatc ggacgcggca cggacgcggg actcggcatc gacgccatcg gctcgccgac
    13021 ctgcggcgcg ggtggctcgg cggcgcgcgc cggtggcgcg acgggcgcgt cctgcaccgg
    13081 cggcggtgct acctgcggtg cgggatgcga aggtggtggc gccggctggc gcaccagctc
    13141 agccagcgtc ggctccggag ccgctgccga agccgccggc gccaccgggc gtcgctgcag
    13201 cgcggcaagt tgctgttcca gcgcgccgac gcgttgcttc aagccggaga tcgacgccag
    13261 cgcgaccacc agcagcacag gcaccgcaag caccgccaac accaacagcg cgatcaagcc
    13321 ttccatcccc actcctcgtg ccgccgtgcc ggcagcctcg gcgatgcccc cggcttttgc
    13381 aacatgacgc ggcgatgcgc aggcctgcgc acccgcatct tcaatcgctg acccggttgc
    13441 ggcccgcggc cttggcgcga tacagcgcgg catcggcgcg acgcagcatc tgttcgacat
    13501 cgtcgtcgcc gggctgcacg ctggccacgc cgatgctgac cgtcatccgt ccttccggcg
    13561 atgccgcatg gacgatatcg gcgctgcgca cccgttcgca cacgcgctgg ccgacacgca
    13621 tcgcttgttc ctgcgtactg gcgggaagga acaccacgaa ttcctcgcca cccggtcggg
    13681 ccagcaggtc gtccgcatgc agcagggcac tgctcaccgc gccggcgaac cggcgcagcg
    13741 cgccatcgcc ctcggcatgc ccgtagagat cgttgtattt cttgaagtga tcgatatcga
    13801 gggcgagcac cgacagttcc gcgcccgtcg cgcgcgcctc cttccacaag cgctcgccgg
    13861 cctcctggaa accgcgataa ttcaggctgc cggtcagggc atcggtgcgg ctgagccggt
    13921 cgaggtcgct gaccgccagg cgcagcgatt cggtgcgctc ctcgatgatg tgctcgcgct
    13981 ggtccagtac ctgctgcaac tcgccgaagg cggcgttcat gcgcgcggcg aggtcgccga
    14041 tcccgcctgc caacggctgc aactcctccg gcatccgggt gagttgcgcg ggatccaggc
    14101 tgccacccag cgcataaccg tgcaggctgg ccagcagccg acccatgctg gcggccagca
    14161 accggcgcag gcgccacagt gcgaacagca cccccagcgt ggtcaccgcg agcagcccga
    14221 acagcagccc cacgcgtggc agcgccggcg ccagcatgcg ctgctccggg gcgatgatcg
    14281 cgaccaccca gccggtgcgc atgaccactg catgcacgta ggcgtcctcg ccgttccgca
    14341 acaagccacc gcgtgtgcgc atctgcccgg gctggccagg ccgtgccgca ccccgacgga
    14401 tcagcgcacc caccgtgccg gtcgcatcga ggaattgcca gcgcagcgcc ttgctcgcat
    14461 ggacgacacg atcctgtcgg tcgagcagca acagctcgaa cccgcgccgc cgcaggttgt
    14521 ccgaccgcac acggatgtac tgctcgatgg ggatggaggc ctgcagcacg ccatcgaacg
    14581 cgccgtcgcg ccgcatcggc gcggaaaccg cgaccaacgg ctcgctgccg tagagccgcg
    14641 cccggtcggc atccgagacg tgcggacgcc ctcccgcgcg gacggcgagg aaccattcgc
    14701 ggtcggccac ccgttcgccg gcaagcgcgg caggccgtcc gttcttcgag cgggccgcga
    14761 cgatgttgcc ttcgcggtcg gtcgccagcg cgcgcagcat cgccgggtac gcacgcaaga
    14821 gcttgccgag ttcctcgtcg agcgttcgtc cggaccgcat cgacccgtcg gccatcaact
    14881 cgacgccgga gagctggccc tgcaggaaat cgtcgatcgc attggcgctg agttgcgcgt
    14941 tggagcccag cctgagcttg aggtcgtcct ggtcgcgttg ccactggctc catagcaggg
    15001 ccatgccgaa cagcagcgcc ggcagcagcg cggtcagcac cagcatgcgg gtgagttcgc
    15061 ggcgcaggct ccatcccgcg attggcgcgt ccggcgtcat gtccacacgc gaaccgccgg
    15121 catgcaggcc gcacaagcag cgaagccccg caggtgcggg gcttcgggct ggcggtcgcg
    15181 gtccgccatc gcgcgcttac gcggcgacga ccttgaccat ttcgaggcac ttgttgctgt
    15241 agccccattc gttgtcgtac cagctgacca gcttgacgaa ggtatcgtcc agcgcgatgc
    15301 cggcctcggc gtcgaagatg ctggtgcgcg catcgccgcg gaaatcggtg gcgaccacct
    15361 tctcgtcggt gtagcccagg atgcccttca gcgcgccttc ggactgcgcc ttcatctccg
    15421 cgcagatctc ggcataggtc gccgggttct cgagttcaac ggtgaggtcg accaccgaga
    15481 cgtcgctggt cggcacgcgg aacgacatgc cggtgagctt gcccttcagc tgcggcagca
    15541 ccaggcccac ggccttggcg gcaccggtgg acgacgggat gatgttctcc aggatgccgc
    15601 ggccgccgcg ccagtccttg ttg
//
LOCUS       DAOOCX010000050        15599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3408_length_15599_cov_9.167589, whole genome shotgun sequence.
ACCESSION   DAOOCX010000050 DAOOCX010000000
VERSION     DAOOCX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15599
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3408_length_15599_cov_9.167589"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK399_06420"
     CDS             <1..332
                     /locus_tag="PK399_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPW12786.1"
                     /translation="WTSSYAVWTAGWATLLLALCHQLFDVRGWRPFGRSMGINAITAY
                     AGSWVMACVMERFGVFGAIYRGVLQPTIGPLLGDKAASLVFAIAFVALWWLLMWAMAK
                     RGIRISV"
     gene            complement(329..1576)
                     /locus_tag="PK399_06425"
     CDS             complement(329..1576)
                     /locus_tag="PK399_06425"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HPW12787.1"
                     /translation="MTTTPLLHDAKVYLVGGAVRDALLGLPAGDHDHVVVGASVQAML
                     DAGFRQVGRDFPVFLHPHTGEEYALARTERKSARGHAGFVVHADPSVTLEDDLRRRDF
                     TLNAIARDADGVLVDPFGGVHDIEARVLRHVGEAFVEDPLRVLRAARFMARFAPLGFA
                     VAPETLALMRQMTASGELADLVPERVWQELAKALRSASPAAFLRTLRDADALRAVLPE
                     VDALYGVPQRAEYHPEVDTGTHVELVCDMAARLAPGDDLVGYAALCHDLGKALTPADT
                     LPAHVMHEQRGVAPVIAMSERLKVPAEHRDVAVLGCREHLNVHRLDELKPATVHEVIA
                     RCDGFRKPKRIDQLATVCEADKRGRTGLSEAPYPQADRLRRLHAAAMAVRSDHIAAQG
                     VTGPAFGEALRKARIAAITEARG"
     gene            complement(1638..4577)
                     /locus_tag="PK399_06430"
     CDS             complement(1638..4577)
                     /locus_tag="PK399_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW12788.1"
                     /translation="MATSAHFRGGRDTGGAGSGTGPGSDFVSALFASDRLDHIEAVAA
                     QEAQSRFGLRHLRIETARDPALDADLQGATPISRLAARTGRLRSQAGQALTFALGDLP
                     RAMLLSVDRPADGRDFDASAAAWRGFLALLTARVATALELQDQRQASERLEKTARLQT
                     ALYTVANLASAGLDMQDTLRRIHDVVGALMYARNFLIALYDGTRDSLRFIYYADEQDL
                     RSVDPEQEYPAARIGDSLTLGVIHTGRAAMGPSRQLRRAFGFDEDAEGYGPSSADWLG
                     VPMVADGVVRGAVVVQSYDQPDRFSQEDQALLEFVAHHILIALTRRQAHDELERRVEE
                     RTLALTEQIRERQRGETLQAALYRIADLASSGLGMEEMLRRIHLVVGELMYARNFYIA
                     LYDGERDSVRFIYFADAKDVNIYETDREVPAADLRDSLTLGLIRHGKPVRGPARDVAE
                     TLHISGIGLGTPALDFLGVPISGDGGARGAVVVQTYEPGVHYSAEDSTLLAYVAQHIM
                     TALERKKLQAELERRVEDRTRELASAVGELREQVKVREQAEHRLIHEALHDALTGLPN
                     RTCFHAALEQALARLHRDDGRRFAVLFLDLDRFKVINDSVGHLFGDMMLKEAGTRLAA
                     CVRGNDMVARLGGDEFAVLMEDIASPEDACHTARRVIEALAEPIHVQGKELFTSASIG
                     ITLGERRYRKAEELLRDADVAMYRAKAHGRQRFEIFDERLHQEALQLLELESDLRHAI
                     QRKEFEPHFQPIVDLRDGGVLGYESLLRWNHPRRGLVPPGEFLKAAEDSGSIEQIDWQ
                     MLANTCRDIRDLPLDGRYVTLNVSPRRFRSPTLAQQLLDQLQAHGIEPACIRIEVTED
                     ALLENPDQVFTTLGTLHQAGVLAALDDFGTGYSSLSYLHRFPLHALKIDRSFVRDLEP
                     MREDGSTAVVRAVLALAGTLGLEVIAEGIETEQQRTCLLELGCTRGQGFLFSHARPAT
                     EWRAAAA"
     gene            complement(4658..6742)
                     /locus_tag="PK399_06435"
     CDS             complement(4658..6742)
                     /locus_tag="PK399_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPW12789.1"
                     /translation="MHKRASFLLAALACGTSLLPAIAHAQAASPPPRSANPPIRVAPP
                     SMAVREAFEAAARGTLDDVLLDSYARHPLGGWLEYTALRKRMDTLPVPRGNAFLARHR
                     GEPVARAFRGDWLAALAARSEWQALLANWESGIDDPGLRCLRLQALQATGRADAAWTT
                     EAQALWRGTGKSLPSSCDAPFAQLATQGALDDGLRWERFDKAAAEGQSGVMRAIARGM
                     TADAALQANAYAAYIDKASADVGTWPKTARSRLVASWGLARLAKASPDQAEALLPGVA
                     QALGFNADERARVLYPIALWTVASYGPDSARRLAAVPEAAYDASLHEWRVREALARSD
                     WRSALAAIGKMDDAQRNDSRWTYLAARTSELTGDAAGAKALYRQAANKTDFHGFLAAD
                     KLDQAYALCPWQPVVAQAAKQAIARDPSIIRALQLFALDRKGWAQREWDDALSRYSDE
                     QRRIAVEVAQDNGWFDRGVFGLVNVGGKRFPEEQRLYLLRFPLHHDATIRRESAKNRL
                     DPAWVAAEIRAESVFDPNARSSADARGLMQVLPATGAGVAAKIGLAWTGGDSLYDADT
                     NIAIGTAYLRQLMDKYGGKPYQVIAGYNAGPAPLGRWMSQRPTMDPDLWIETISYKET
                     REYVARVLSFSTLYDWRLNGDAVRLTDRMQGLPNGQRKGFACATPDAPKPAQTPAPAA
                     PTKPTLKDRRRH"
     gene            6792..7595
                     /locus_tag="PK399_06440"
     CDS             6792..7595
                     /locus_tag="PK399_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPW12790.1"
                     /translation="MAALLIYLLLGACAGLLAGLLGIGGGLVLVGALAWLLPMQGVPA
                     DAAMHVALASSLASIVLTAAASARAHHRRGSVLWPTVAWMVPGVLLGGWLGSRFAIAL
                     DDGVLRWCVAGYCFLIAAQMLWSKTPADSGMVVAPRGPGYTLAGSGIGIVSAIVGIGG
                     GSLTVPLLVWRGVAPVRAVGTSSACGIFIGIGSALGYALQAPTGAVALPGAFGYVYLP
                     AALGVALASVFAAPYGTRLAHALSGLALKRVFAVFLLLVGGAFAYSALT"
     gene            7616..8599
                     /locus_tag="PK399_06445"
     CDS             7616..8599
                     /locus_tag="PK399_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPW12791.1"
                     /translation="MRSVLHATFGEPADVLTAGDSPMPEPGPGQARVRMILSPIHNHD
                     VWTVRGQYGYKPALPAIAGSEAMGVVDALGEGVDNVTIGQRVTTAGARGAWAEYFTVP
                     ANSLVPVPDNIDDATAAQLVAMPLSALMLLETLGVVEGDWIVQNAANGAVGKALAMLA
                     RARGIHVLNLVRREEGVAEMAAQGIGNTVCTMHEGWKATARELMGGARPKAAVDSVGG
                     PASGVLASLLGENGLLVSFGSMTGESMQIPSGELIFKQITVKGFWGTPASKALSSQDR
                     RRLMGELLQRAASGELQLPVAATYGFDAIGDAIRDSLVPGRASKGKILLRP"
     gene            complement(8609..9346)
                     /locus_tag="PK399_06450"
     CDS             complement(8609..9346)
                     /locus_tag="PK399_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005419292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPW12792.1"
                     /translation="MLSANIQAGSSTRGYQDYVTRSWSHVLPAGNKRGALDTIAELAG
                     RHDIVGLQESDPGSLRSGFTNQTHYLAQRGGFAYWSHQPNRRMGGVASSANGLLSKLE
                     PKRVEEHSLPGRVKGRGVLIGTFGNGDDALTVAVAHLSLGSGSRKSQIDFIAELLGDH
                     PHAVLMGDFNCDPDQPEMQGLYRNTRLQPPADCVPTFPSWRPQRAIDHVLVTPALAIA
                     TMQAMPAAHSDHLALSVELDVPEHALG"
     gene            complement(9387..10265)
                     /locus_tag="PK399_06455"
     CDS             complement(9387..10265)
                     /locus_tag="PK399_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HPW12793.1"
                     /translation="MTASRYALLLLALLPLAACKNDAPAPVTETPTTETATNVKQASA
                     EDPARKAAAEAALKAAEEAAAKAPPLVAGVDYADIPNGQPFETTDGRIEVVEVFGYVC
                     PFCAAVQPTVRAMKEKFPADVHMVYVPAAFGGPWDNYAKGYYAAEAMGLVDKTHDAMF
                     RAIHIDKTLKGERGMDTPEEIAAFYSAYGADPKQFASSMQSFAVATKINRGKQFILGA
                     FSNGDSPSTPAFIVNGKYRVKGKSVDDVFRVLNQLIVAERAKNASSAPTAAPAATDAA
                     APAAPAEAPAAAAPQA"
     gene            complement(10286..10984)
                     /locus_tag="PK399_06460"
     CDS             complement(10286..10984)
                     /locus_tag="PK399_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HPW12794.1"
                     /translation="MRVLLLSLALLLSAFALPAQAQQQLPGLTEGSEYRYIEDGKPYR
                     AVPGKIEVAEVFGYWCPHCAHFAPMLEQWKATMPANAQLVLVPAMFDPNDAYARLFFI
                     AEKAKAVDATHARLFRAIHETEDLPRQATIPQLTAWYLRQPGVNAKAFAAAQADEATL
                     QAKAANAREFQMRSDIPGTPSLVIAGKYLVLGNSFESMLDNARRVILAASPAAKAKPT
                     PTKPKAAPAAKPRT"
     gene            complement(11066..11836)
                     /locus_tag="PK399_06465"
     CDS             complement(11066..11836)
                     /locus_tag="PK399_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPW12795.1"
                     /translation="MRLARVHGLVGVACLVLAAAAYAQSTAVAVPDNAPVQAAPLDDK
                     PATWGDAKAGQSKAGACAACHGVDGNAMQQNAPRIAGMPERYIAQQLALFKHGERTTG
                     LAGLMAPYAAPLSNQDMRDLGAWFATQKAGSGVADDTVIAAGPSKGMKFYQVGERLYR
                     GGDAARGIPACMACHGPAGAGNPGPAYPALHGQETGYVVRRLEEYRAGTTTYKDAAHF
                     NLMASVAKPLSDEEIRSLGSYVQGLHARADATQAPASH"
     gene            12017..12628
                     /gene="yihA"
                     /locus_tag="PK399_06470"
     CDS             12017..12628
                     /gene="yihA"
                     /locus_tag="PK399_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPW12796.1"
                     /translation="MAAPNPFNRADYLLAAHTHRQLPPDGGREVAFAGRSNAGKSSAL
                     NALCGRNALARVSKTPGRTQQLVYFGLPPHESKYLVDLPGYGYAKVPQDLQAHWQAFL
                     DGYFGSRDALAGLVVVMDIRHPLKDYDRSMIGYAMRRGLPAHALLTKADKLSRGAAMN
                     TAQAVRKDLSSSYGDSVSVQVFSGETKQGVDELRSVVAGWLEI"
     gene            complement(12796..14637)
                     /locus_tag="PK399_06475"
     CDS             complement(12796..14637)
                     /locus_tag="PK399_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPW12797.1"
                     /translation="MRTILCTLALLLGMSTPARAIDIERIEPTNWWVGMRHNRVELLV
                     HGTGIATSTPRLSRAGVAIVDVQKTDNPNYLFVTVEIAGDAIPGGIDIEFVHAGKVVA
                     THPWRLDAREPGSARRRGFDARDAIYLVTPDRFANGDPSNDSVTGMLEAANRANPNGR
                     HGGDIAGMRQHIDYIAGMGFTQLWPTPFLENDQPQYSYHGYAITDLYKTDPRMGRNED
                     VRALSREARAKGVGLIMDIVLNHIGSKHWWMTDLPAKDWINHGGTFAPTNHRRTTIQD
                     PHAAPADREAFTDGWFVDAMPDLNQRNPHLARYLIQNTLWWIEYAGLSGIREDTFGYA
                     DTQFLGDWAKAVLAEYPDFAMVGEEWSINPVVVAHWQRGKQNTSGHVPHMPSMMDFPI
                     HIALRSALAQPEGWDSGWVNLYEMLANDFVYPDADALVVFAENHDTSRLFAHLDGDLA
                     TWKLGMAYLATVRGTPQFYYGSEVLLRGPRERNDGQLRADMPGGWSGDPADAFTGNGL
                     SAAQRDAQAYTRALFDWRKRTPLLHSGKLTHFAPQDGVYVYFRHDGAKSVMVALNKNA
                     GATPLLLERFDAFLRGRGARDAWTGKAVALGTTLALPGKSATLLEIE"
     gene            14807..>15599
                     /locus_tag="PK399_06480"
     CDS             14807..>15599
                     /locus_tag="PK399_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HPW12798.1"
                     /translation="MSGPSNANDTPITDYRQLVEVMASGEKPIEAWRIGTEHEKFGFR
                     LDDLRPPTFDGERGIEALLKGLTRFGWTPYEESVDGQPARVIALTKGMASVTLEPAGQ
                     LELSGAPVETIHDTCKEVGSHLREVRAVADELQLGFLGMGFQPKWRRDEMPWMPKGRY
                     RIMRAYMPKVGNLGLDMMTRTCTVQVNLDYASEADMVKKFRVSLALQPVATALFADSP
                     FTEGKPNGQLSYRSHIWTDTDPDRTGMLDFVFEDGFGYERYVDYLL"
BASE COUNT         2225 a         5191 c         5609 g         2574 t
ORIGIN      
        1 tgtggacgtc gtcctacgcg gtgtggacgg cgggttgggc gaccctgctg ctggcgctgt
       61 gccatcagct gttcgatgtg cgtggctggc gaccgttcgg ccgcagcatg ggcatcaacg
      121 cgatcaccgc gtacgcgggc tcgtgggtca tggcctgcgt gatggagcgc ttcggcgtgt
      181 tcggcgcgat ctatcgcggt gtactgcaac ccaccatcgg cccgttgctg ggcgacaagg
      241 ccgcatcgct ggtgttcgcg atcgccttcg tggcgttgtg gtggctgttg atgtgggcga
      301 tggcgaagcg cgggatccgg atcagcgtct agccgcgcgc ttcggtgatc gccgcgatgc
      361 gtgccttgcg cagggcctcg ccgaaggccg gaccggtgac gccctgtgcg gcgatgtggt
      421 cgctgcggac cgccattgcg gcggcgtgca ggcgacgcaa gcgatcggct tgcggatacg
      481 gagcctccga caggccggtg cgtccgcgct tgtcggcttc gcagacggtc gcgagctggt
      541 cgatccgctt cggcttgcgg aaaccgtcgc aacgcgcgat cacctcgtgc acggtcgccg
      601 gcttgagttc gtccaggcga tggacgttga ggtgctcgcg gcagcccagc acggccacgt
      661 cgcgatgttc ggccggcacc ttcaggcgtt cgctcatcgc gatgaccggc gcgacgccgc
      721 gctgttcgtg catcacgtgc gcgggcaagg tatcggcggg ggtgagtgcc ttgcccaggt
      781 cgtggcagag cgcggcgtag ccgaccaggt catcgcccgg cgccagccta gcggccatgt
      841 cgcagaccag ttcgacgtgc gtgccggtgt cgacttccgg atggtactcc gcgcgctgcg
      901 gcacgccgta cagcgcatcc acttccggca gcaccgcgcg caacgcgtcg gcatcgcgca
      961 gggtgcgcag gaacgcggcg ggcgacgcgc tgcgcagggc cttcgccagt tcctgccaga
     1021 cccgttcggg caccaggtcg gccagttcgc cactggccgt catctggcgc atcagcgcca
     1081 gcgtctccgg cgcgacggcg aagccgagcg gcgcgaagcg ggccatgaag cgcgccgcgc
     1141 gcaacacgcg aaggggatcc tcgacgaaag cctcgccgac atgccgcagc acgcgcgctt
     1201 cgatatcgtg cacgcccccg aacggatcga cgagcacgcc atcggcatcg cgcgcgatcg
     1261 cgttgagggt gaagtcgcgg cgacgcaggt cgtcttccag cgtcaccgat gggtcggcat
     1321 gcaccacgaa gcccgcatgc ccgcgcgcgg atttgcgctc ggtgcgcgcc agcgcgtatt
     1381 cctcgccggt gtgcggatgc aggaagacgg ggaagtcgcg gccgacctgc cggaaaccgg
     1441 catccagcat ggcctgcacg ctggcgccga ccaccacgtg gtcatggtcg cccgcaggca
     1501 ggccgagcag ggcgtcgcgc accgcgccgc cgaccaggta gaccttcgcg tcgtggagga
     1561 gcggtgtcgt cgtcatccgc cgatgatgcc tgatccgctg cgcatgcctg cagccgcgac
     1621 atgcaggcgt gctgcgttca ggcggccgcg gcgcgccact ccgtcgccgg tcgcgcatgc
     1681 gagaacagga agccctggcc acgcgtgcag cccaactcaa gcaggcaggt gcgctgctgt
     1741 tcggtctcga tgccttcggc gatcacttcc aggcccaggg tcccggccag tgccagcacc
     1801 gcgcgcacca ccgcggtgct gccgtcctcg cgcatcggtt cgaggtcgcg cacgaacgag
     1861 cggtcgatct tcaatgcatg cagcgggaag cgatggaggt agctcaacga ggaatagccg
     1921 gtgccgaaat cgtccagcgc cgccagcacg ccggcctggt gcagcgtgcc cagggtggtg
     1981 aagacctggt cggggttctc cagcagcgcg tcctcggtga cctcgatgcg gatgcatgcg
     2041 ggttcgatgc catgcgcctg caactggtcg agcagttgct gggcgagggt cggcgagcgg
     2101 aaccgccgcg gcgatacgtt gagggtcacg tagcgcccat ccagcggcag gtcgcggatg
     2161 tcgcggcagg tgttggccag catctgccag tcgatctgct cgatgctgcc gctgtcctcc
     2221 gccgccttca ggaactcgcc cggcggcacc agcccgcggc gcgggtggtt ccagcgcagc
     2281 agcgattcat agccgagcac gccgccgtcg cgcaggtcca cgatcggctg gaaatgcggt
     2341 tcgaattcct tgcgctggat cgcatgccgc aggtcgcttt ccagctccag caactgcagc
     2401 gcctcctggt gcaggcgctc gtcgaagatc tcgaagcgct ggcgtccgtg cgccttggcg
     2461 cgatacatcg ccacgtcggc gtcgcgcagc agttcctcgg ccttgcgata gcggcgttcg
     2521 cccagggtga tgccgatgct ggccgacgtg aacagctcct tgccctgcac gtggatcggt
     2581 tcggccaatg cctcgatcac ccggcgcgcg gtgtggcagg cgtcctcggg gctggcgata
     2641 tcctccatga gcacggcgaa ctcgtcgccg cccagccggg ccaccatgtc gttgccgcgt
     2701 acgcaggcgg ccaggcgcgt gccggcttcc ttcagcatca tgtcgccgaa caggtggccg
     2761 acgctgtcgt tgatcacctt gaagcggtcg aggtcgagga acagcacggc gaagcgccgg
     2821 ccgtcgtcgc gatgcagccg cgccagcgcc tgctccagcg cggcgtggaa gcaggtgcgg
     2881 ttcggcagcc cggtcaatgc atcgtgcagg gcttcgtgga tcagccggtg ctcggcctgt
     2941 tcgcgcacct tgacctgttc gcgcagttcg ccgaccgcgc tggcgagttc gcgggtgcga
     3001 tcttccaccc ggcgctcgag ttcggcctgc agcttcttgc gttcgagcgc ggtcatgatg
     3061 tgctgcgcca cgtaggccag cagggtgctg tcctccgcgc tgtagtgcac gccgggctcg
     3121 taggtctgca ccaccaccgc gccacgcgcg ccgccgtcgc cgctgatcgg cacgccgagg
     3181 aaatccagcg cgggcgtgcc caggccgatg ccgctgatgt gcagggtctc cgccacgtcg
     3241 cgcgcggggc cacggaccgg cttgccgtgg cggatcaggc ccagggtgag gctgtcgcgc
     3301 aggtcggcgg ccggcacctc gcggtcggtt tcatagatgt tgacgtcctt ggcgtcggcg
     3361 aaatagatga agcgcacgct gtcgcgttcg ccgtcgtaca gggcgatgta gaaattgcgc
     3421 gcgtacatga gctcacccac caccaggtgg atgcgccgca gcatctcttc catgccaagg
     3481 ccgctgctgg cgaggtcggc gatgcggtac agcgccgcct gcagggtctc gccacgctgg
     3541 cgctcgcgga tctgttcggt gagcgcgagg gtgcgctcct cgacgcggcg ttcgagttcg
     3601 tcgtgcgcct ggcgccgggt cagcgcgatc aggatgtggt gggcgacgaa ctcgaggagg
     3661 gcctggtctt cctggctgaa gcgatcgggc tggtcgtagc tctgcaccac caccgcacca
     3721 cgaaccacgc catcggcgac catcggcacg ccgagccagt cggcgctgct gggtccataa
     3781 ccttccgcgt cctcgtcgaa gccgaatgcg cggcgcaatt gccgcgatgg ccccatcgcc
     3841 gcacgcccgg tgtggatcac gcccagggtc aggctgtcgc cgatccgcgc ggccgggtat
     3901 tcctgctccg ggtcgacgct gcgcaggtcc tgttcgtcgg cgtagtagat gaagcgcagg
     3961 ctgtcgcgcg tgccgtcgta cagcgcgatc aggaaattgc gcgcatacat cagggcgccg
     4021 accacgtcat ggatgcgccg cagggtgtcc tgcatgtcca gtcccgcgga cgcgaggttg
     4081 gccacggtgt acagcgcggt ctgcaggcgc gcggtctttt ccaggcgctc cgaggcctgc
     4141 cgctggtcct gcaattccag cgcggtggcg acgcgcgcgg tgagcagggc gaggaagccg
     4201 cgccacgctg ccgccgaggc atcgaagtcg cggccatcgg ccgggcgatc caccgacagc
     4261 agcatcgcgc gcggcaaatc gccaagcgcg aacgtcaatg cctggccggc ttgcgaacgc
     4321 aggcgtccgg tcctggccgc cagccgcgag atgggcgtgg cgccctgcag gtcggcatcg
     4381 agcgcgggat cgcgtgcggt ctcgatccgc aggtggcgca ggccgaagcg tgattgcgct
     4441 tcctgcgcag caacggcctc gatgtggtcg aggcgatcgc tggcgaacag cgccgatacg
     4501 aaatcactgc cgggtcccgt tccggagccg gcgccgcccg tatcgcgccc tcccctgaaa
     4561 tgggcgctcg tggccatacg ccaccgaagg tacgcctatc cccgcgcgca cggcaagcgc
     4621 ggcgtgcgcc aagcggacga gcggcgctgc gccggtttca gtggcggcgc cgatccttga
     4681 gcgtgggctt cgtcggcgcc gccggcgcgg gtgtttgcgc gggttttggc gcgtcgggcg
     4741 tggcgcaggc gaagcccttg cgctggccgt tcggcaatcc ctgcatgcgg tcggtcaggc
     4801 gcacggcatc gccgttgagc cgccagtcgt agagcgtgct gaagctcagg acgcgggcca
     4861 cgtattcgcg ggtttccttg tagctgatgg tctcgatcca gaggtccgga tccatcgtcg
     4921 ggcgctgcga catccagcgc ccaagcggcg ccgggccggc gttgtagccg gcgatcacct
     4981 ggtagggctt gccaccgtat ttgtccatca gctggcgcag gtaggcggtg ccgatggcga
     5041 tgttggtatc ggcgtcgtac aggctgtcgc cgccggtcca tgccaggccg atcttcgccg
     5101 cgacaccggc accggtggcc ggcaagacct gcatcagccc gcgcgcatcc gcggacgagc
     5161 gcgcgttcgg atcgaacacg gattcggcgc ggatctcggc ggcgacccag gccggatcga
     5221 ggcggttctt cgcggactcg cggcggatgg tggcgtcgtg gtgcagcggg aagcgcaaca
     5281 ggtacaggcg ctgttcctcg ggaaagcgct tgccgcccac gttgaccagg ccgaacacgc
     5341 cgcggtcgaa ccagccgttg tcctgcgcca cttctaccgc gatccggcgc tgctcgtcgg
     5401 agtagcgcga cagcgcatcg tcccactcgc gctgcgccca gcccttgcgg tccagcgcga
     5461 acagctgcaa tgcgcggatg atcgacggat cgcgcgcgat ggcctgcttc gcggcctgcg
     5521 ccaccaccgg ctgccacggg catagcgcat acgcctggtc gagcttgtcg gcggccagga
     5581 agccgtggaa atcggtcttg ttcgccgcct gccgatacag cgccttcgca cccgcggcat
     5641 cgccggtcag ttcgctggtg cgcgcggcga ggtaggtcca gcgcgaatcg ttgcgctgcg
     5701 catcgtccat cttgccgatc gcggccagcg cgctgcgcca gtcgctgcgc gccagcgctt
     5761 cgcgcacgcg ccattcgtgc aggctcgcgt cgtaggcggc ttcgggcacc gccgcgagcc
     5821 ggcgtgcgga gtccggcccg tacgaggcca ccgtccacag tgcgatcgga tacaggacgc
     5881 gggcgcgttc atcggcattg aaaccgaggg cctgcgccac gccgggcagc agcgcttccg
     5941 cttggtcggg cgatgccttc gccaaccgtg ccaaccccca cgaggcgacc aggcggctgc
     6001 gcgcggtctt cggccatgtg ccgacatcgg ccgaggcctt gtcgatgtag gccgcatacg
     6061 cattcgcctg cagggccgcg tcggccgtca tgccgcgtgc aatggcacgc atgaccccgg
     6121 actggccttc ggccgcggcc ttgtcgaagc gctcccagcg cagcccatcg tcgagcgcgc
     6181 cctgggttgc cagctgcgcg aacggcgcat cgcaggacga tggcaacgac ttgccggtgc
     6241 cacgccacag ggcctgcgcc tccgtggtcc aggcggcatc cgcacggccc gtcgcctgca
     6301 gcgcctgcag gcgcaggcaa cgcaggccgg gatcgtcgat gccggactcc cagttcgcca
     6361 gcaaggcctg ccattcgctg cgcgcggcga gtgccgcgag ccagtcgcca cggaatgcgc
     6421 gtgccaccgg ctcgccgcga tggcgggcca ggaaggcatt gccgcgcggc accggcaagg
     6481 tgtccatgcg cttgcgcagc gcggtgtatt ccagccagcc gcccagcgga tgccgcgcat
     6541 acgaatcgag caggacgtcg tcgagggtcc cgcgtgcagc ggcctcgaag gcctcgcgca
     6601 cggccatgct gggcggggcg acccggatcg gcggattggc gctgcgcggc ggtggcgagg
     6661 cggcttgggc gtgggcgatg gcgggcagca ggctggtgcc gcacgccagc gcggccagca
     6721 ggaacgaggc gcgcttgtgc atcgccggac tatacccgag catcgatgaa tcacttttca
     6781 ggtggcatcg catggccgcg ctgttgatct atctgttgct cggcgcgtgc gcgggcctgc
     6841 tcgcgggcct gctcggcatc ggcggcgggc tggtgctggt gggtgcgctt gcgtggttgc
     6901 tgccgatgca gggcgtgccc gccgacgcgg cgatgcacgt ggccttggcg agctcattgg
     6961 ccagcatcgt gctcaccgct gccgcttcgg cgcgtgcgca tcaccgacgc ggcagcgtgc
     7021 tgtggccgac ggtggcgtgg atggtgccgg gcgtgctgct gggtggctgg ctgggcagtc
     7081 gcttcgccat cgcactggat gacggggtgt tgcgctggtg cgtggccggc tactgcttcc
     7141 tgatcgcggc gcagatgctg tggtcgaaga cgcccgcgga cagcggcatg gtcgtcgcac
     7201 cccgcggccc gggctacacc ttggcaggaa gcggcatcgg catcgtctcg gcgatcgtcg
     7261 gcatcggcgg cggcagcctg accgtgccat tgctggtgtg gcgcggcgtg gcgccggtgc
     7321 gcgcggtcgg cacgtcgtcc gcctgcggga tcttcatcgg catcggcagc gcgctggggt
     7381 atgccttgca ggcaccgacg ggtgccgtcg ccttgccggg cgcattcggt tacgtgtacc
     7441 tgcccgcggc gctgggcgtg gcgctggcct cggtgttcgc cgcgccgtac ggcacccggc
     7501 tggcgcacgc gctgagcggg ctggcgctga agcgggtgtt cgcggtgttc ctgctgctgg
     7561 tcggcggggc cttcgcgtac agtgcgctga cctgaaccat acagtggagt acgtgatgcg
     7621 cagtgtcctg catgcgacct tcggcgaacc ggccgacgtc ctgaccgccg gcgacagccc
     7681 gatgccggaa cccggtcccg gacaggcgcg ggtgcgcatg atcctgtcgc cgatccacaa
     7741 ccacgacgtc tggaccgtgc gcgggcagta cggctacaag cccgcgttgc cggcgatcgc
     7801 cggcagcgag gcgatgggcg tggtcgatgc actgggcgag ggcgtcgaca acgtgaccat
     7861 cggccagcgc gtgaccacgg ccggcgcgcg tggcgcgtgg gcggaatatt tcaccgtccc
     7921 ggcgaattcg ctggtgccgg tcccggacaa catcgacgat gcgaccgcgg cgcagctggt
     7981 ggcgatgccg ctgagcgcgc tgatgctgct ggaaacgctg ggcgtcgtgg aaggcgactg
     8041 gatcgtgcag aacgccgcca acggcgcggt cggcaaggcg ctggcgatgc tggcgcgtgc
     8101 acgcggcatc cacgtgctga acctggtgcg ccgcgaggaa ggcgtggccg aaatggcggc
     8161 gcagggcatc ggcaataccg tctgcaccat gcacgagggc tggaaggcga ccgcgcgcga
     8221 gctgatgggc ggggcgcgcc cgaaggctgc ggtggattcg gtcggcggcc cggccagcgg
     8281 cgtgctggcc agcttgctgg gcgaaaacgg cctgctggtg tcgttcggta gcatgaccgg
     8341 cgagtcgatg cagatcccct cgggcgaact gatcttcaag cagatcacgg tcaagggctt
     8401 ctggggcacg ccggccagca aggcgctgtc gtcgcaggat cgccgccggt tgatgggcga
     8461 gctgctgcag cgcgcggcca gcggggaatt gcagctgccg gtcgcggcga cctatggctt
     8521 cgacgccatc ggcgacgcga tccgcgattc gctggtgccg ggccgggcga gcaagggcaa
     8581 gatcctgctg cgtccctgag cgccacgatc agccgagggc gtgctccggc acgtccagct
     8641 ccaccgacaa cgccaggtgg tcggaatgcg ccgccggcat cgcctgcatg gtggcgatgg
     8701 ccagggccgg cgtgaccagc acgtggtcga tcgcccgctg cggccgccac gaggggaagg
     8761 tcggcacgca atccgccggt ggctgcaggc gggtgttgcg gtagaggccc tgcatttccg
     8821 gctggtccgg gtcgcagttg aagtcgccca tcagcaccgc gtgcgggtgg tcgcccagca
     8881 actcggcgat gaagtcgatc tgcgacttgc gcgatccgct gcccagcgac agatgcgcta
     8941 cggccacggt cagcgcgtca tcgccattgc cgaaggtgcc gatcaacacg ccgcgaccct
     9001 tgacccggcc gggcagggaa tgctcttcga cccgtttcgg ttcgagcttg ctgagtagtc
     9061 cgttcgcact cgaggcgaca ccgcccatcc gccggttcgg ttggtggctc cagtaggcga
     9121 agccaccgcg ttgcgccagg tagtgggtct ggttggtgaa gccggaacgc aggctgcccg
     9181 gatccgactc ctgcaggccg acgatgtcgt ggcggcccgc aagttcggca atggtgtcca
     9241 gcgcgccgcg cttgttgccg gcgggcaaca cgtgcgacca gctgcgggtc acgtagtcct
     9301 ggtagccgcg ggtgcttgaa cccgcctgga tgttggcgct cagcaggcgc agccggcgcg
     9361 tctccatgcg gcgggtgatg ccgggatcag gcttgcggcg cagcggcggc aggtgcctcg
     9421 gccggcgccg cgggtgctgc ggcatcagtt gccgccggtg ccgccgtggg cgcggaagat
     9481 gcgttcttcg cgcgctcggc cacgatcaac tggttgagca cgcggaacac atcgtcgacc
     9541 gacttgccct tgacccggta cttgccgttg acgatgaagg ccggggtgct gggcgagtcg
     9601 ccgttgctga aggcgccgag gatgaactgc ttgccgcggt tgatcttggt ggccaccgcg
     9661 aagctctgca tgctgctggc gaactgcttg ggatcggcgc catacgctga gtagaacgcg
     9721 gcgatctctt ccggcgtgtc catcccgcgc tcgcccttca gggtcttgtc gatgtggatt
     9781 gcgcggaaca tcgcgtcatg ggtcttgtcg accaggccca tcgcctcggc ggcgtagtac
     9841 cccttggcgt agttgtccca cgggccaccg aacgcggccg gcacatagac catgtgcacg
     9901 tccgccggga atttttcctt catcgcgcgc acggtgggct gcacggctgc gcagaacggg
     9961 cagacgtagc cgaacacctc gaccacctcg atgcgcccat cggtggtctc gaacggctgg
    10021 ccattcggga tatcggcgta atccacgccc gcgaccagcg gcggcgcctt cgcggcggct
    10081 tcctcggcgg ccttgagcgc ggcttccgcg gccgccttcc gagcggggtc ctcggcgctg
    10141 gcctgcttca cgttggtggc ggtttcggtg gtcggggttt cggtgaccgg ggccggtgcg
    10201 tcgttcttgc aggcggccag cgggagcagg gcgaggagca gcagggcgta acgcgatgcg
    10261 gtcatggcgg ttccggtcga tgggttcagg tgcggggctt ggcagcaggc gccgctttcg
    10321 gcttggtcgg cgtgggcttg gctttcgcgg ccggagacgc cgccaggatc acgcggcggg
    10381 cgttgtcgag catgctctcg aagctgttgc ccaggaccag gtacttgccg gcgatgacca
    10441 gcgagggcgt ccccgggatg tcgctgcgca tctggaactc gcgcgcgttg gcggccttcg
    10501 cctgcagggt cgcttcatcg gcctgggccg ccgcgaacgc cttcgcgttc acgcccggct
    10561 ggcgcaagta ccaagcggtc agttgcggga tggtggcttg ccgcggcagg tcttcggttt
    10621 cgtggatcgc gcggaacagg cgggcatggg tcgcgtcgac cgccttggcc ttttcggcga
    10681 tgaagaacag gcgcgcatag gcgtcgttcg ggtcgaacat ggccggcacc agcaccaact
    10741 gagcattcgc cggcatggtc gccttccact gctccagcat cggcgcgaaa tgcgcgcagt
    10801 gcgggcacca gtagccgaac acctcggcga cctcgatctt gccggggacg gcgcggtacg
    10861 gcttgccgtc ctcgatgtag cggtattcgc tgccctcggt cagcccgggc aactgctgct
    10921 gcgcttgggc gggcagggcg aaggcggaca gcagcagggc caaggacaac agcaggacgc
    10981 gcatcgactc gactccggcg atcaggtggg ccgggcagct tgcgcaggcc cggcggaacg
    11041 catggtgaag ccgcgccgcc gtggatcagt ggctggcggg cgcctgggtc gcgtcagcgc
    11101 gggcatgcag gccctgcacg tagctgccca gcgagcggat ttcctcgtcg ctgagcggct
    11161 tggcgacgct ggccatcagg ttgaagtgcg cggcgtcctt gtaggtcgtg gtaccggcgc
    11221 ggtactcctc gaggcgccgc accacgtagc cggtttcctg cccgtgcagc gccgggtagg
    11281 ccggtccggg attgccggcg ccggcggggc cgtggcaggc catgcaggcg gggatgccgc
    11341 gggcggcgtc gccaccccgg tagaggcgtt cgcccacctg gtagaacttc atgcccttgc
    11401 tcgggccggc cgcgatgacg gtgtcgtcgg cgacgccgga gccggctttc tgggtggcga
    11461 accacgcgcc gaggtcgcgc atgtcctggt tggacagcgg cgcggcatac ggcgccatca
    11521 gtccggccag gccggtggtg cgttcgccat gcttgaacag cgcgagttgc tgggcgatgt
    11581 agcgttccgg catgcccgcg atccgcggcg cgttctgctg catcgcgttg ccgtccacgc
    11641 cgtggcaggc ggcgcaggca ccggccttgg actggccggc cttggcatcg ccccaggtcg
    11701 cgggcttgtc atccagcgga gcggcctgga ccggggcgtt gtcgggaacc gcaacggcgg
    11761 tgctctgggc atatgcggcc gcagccagca ccaggcaggc aacgccgaca agaccatgga
    11821 cgcgggcaag acgcatgcgg aactccaata ccaatgcggg tgcggccgaa accgctgaac
    11881 cggcgaatta tcgtcgggcg gcgcatgacg gtcaaaccgc gcgcggtggc ccgggccgtc
    11941 gatcgccggg cccctggtcc cctccgcgac ggcccgcgtg gcgggctgcg acgccggggc
    12001 gtgcgatcct atgcgcatgg ctgcccccaa tccgttcaac cgcgccgact acctgctggc
    12061 tgcgcacacc caccgccaac tgccgcctga cggtggccgc gaggtcgcct tcgccggccg
    12121 ttccaacgcc ggcaagtcgt ccgcgctgaa cgcgctgtgc ggccgcaacg cgctggcgcg
    12181 ggtctccaag acgccgggcc gcacccagca actggtgtat ttcggcctgc cgccgcacga
    12241 gtccaaatac ctggtcgacc tgccgggtta cggctacgcc aaggtcccgc aggacctgca
    12301 ggcgcactgg caggcgttcc tcgacggcta tttcggcagc cgggatgcgc ttgccggcct
    12361 ggtggtggtg atggacatcc gccatccgct gaaggactac gaccgctcga tgatcggtta
    12421 cgcgatgcgc cgcggcctgc ccgcgcatgc cttgctgacc aaggccgaca agctctcgcg
    12481 cggggcggcg atgaataccg cgcaggcggt gcgcaaggat ctgtcgtcaa gttatggcga
    12541 cagcgtcagc gtccaggtct tctccggcga gaccaagcag ggcgtggacg aattgcgttc
    12601 ggtcgtggcc ggctggctgg agatctaggg gcggatcctc gagggtgccg gcgcctaccg
    12661 ccggctgatc gctacgcggc tcgcatgccg ctgcgccccg gccatccggc ttgcatgccg
    12721 gatggcgttc gcgggctcgc ggccctgccg ctcgaaaccc ccgctcaccc cgacggtttc
    12781 gccgtcgtga tgtggctact cgatctcgag cagcgtggcc gacttgcccg gcaacgcgag
    12841 cgtcgtgccc agtgcgaccg ccttgcccgt ccatgcatcg cgcgcgccgc gcccgcgcag
    12901 gaaggcgtcg aagcgctcca gcagcaacgg cgtcgcgccc gcattcttgt tcagcgccac
    12961 catcaccgat ttggcgccat cgtggcggaa gtacacgtac acgccgtcct gcggggcgaa
    13021 gtgggtgagc ttgccggagt gcagcagcgg ggtgcgtttg cgccagtcga acagcgcacg
    13081 ggtgtaggcc tgcgcgtcgc gctgcgcggc gctcaatccg ttgccggtga aggcgtccgc
    13141 gggatcgccg gaccagccgc cgggcatgtc ggcgcgcaac tggccgtcgt tgcgttccct
    13201 tgggccacgc agcagcactt cggagccgta gtagaactgc ggcgtgccgc gcaccgtggc
    13261 caggtacgcc atgccgagct tccatgtcgc caggtcgccg tcgaggtggg cgaacaggcg
    13321 cgaggtgtcg tggttctcgg cgaacaccac cagcgcatcg gcatccggat agacgaaatc
    13381 gttggccagc atctcgtaga ggttcaccca gccgctgtcc cagccttcgg gctgggcaag
    13441 cgcgctgcgc agggcgatgt ggatggggaa gtccatcatg ctcggcatgt gcgggacatg
    13501 gccgctggtg ttctgcttgc cgcgctgcca atgcgcgacg acgaccgggt tgatgctcca
    13561 ttcctcgccg accatcgcga agtccgggta ctcggccagc accgccttcg cccaatcgcc
    13621 caggaactgc gtgtccgcgt agccgaaggt gtcctcgcgg atgccggaca gcccggcgta
    13681 ctcgatccac cacaaggtgt tctggatgag atagcgcgcc aggtgcgggt tgcgctggtt
    13741 gaggtcgggc atggcgtcga cgaaccagcc gtcggtgaag gcctcgcggt ccgcgggcgc
    13801 cgcatgcgga tcctggatcg tggtgcggcg gtggttggtg ggggcgaacg tgccgccgtg
    13861 gttgatccag tccttcgccg gcaggtcggt catccaccag tgcttcgaac cgatgtggtt
    13921 gagcacgatg tccatgatca ggccgacgcc cttcgccctc gcctcgcgcg acagcgcacg
    13981 cacgtcctcg ttgcgaccca tgcgcggatc cgtcttgtag agatcggtga tcgcgtagcc
    14041 gtggtagctg tattgcggct ggtcgttttc caggaagggc gtcggccaca gctgggtgaa
    14101 gcccatcccg gcgatgtagt cgatgtgctg gcgcatgccg gcgatgtcgc cgccgtggcg
    14161 gccgttggga ttcgcgcggt tggccgcttc aagcatgccg gtgacgctgt cgttcgatgg
    14221 atcgccattg gcgaaccggt ccggcgtcac caggtagatc gcatcgcgcg catcgaagcc
    14281 gcggcgtcgc gcggaacccg gttcgcgcgc gtcgagccgc cagggatgcg tcgccacgac
    14341 cttgccggcg tggacgaatt cgatgtcgat gccgcccggt atcgcatcgc cggcgatctc
    14401 gacggtgacg aacaggtagt tcgggttgtc ggtcttctgc acgtcgacga tggccacgcc
    14461 cgcacgcgac agtcgcggcg tcgatgtcgc gatacccgtg ccatgcacca gcaactcgac
    14521 ccggttgtgg cgcatgccca cccaccagtt ggtcggttcg atgcgttcga tgtcgatggc
    14581 acgcgccggc gtcgacatgc cgagcagcaa cgcaagcgtg cagaggatcg tgcgcaccag
    14641 gcccctccca aggcagcgat gtgccaggga ggctacaagc gcgacccggc atggacaacg
    14701 catgccgccg ggaatacgta tgcagccgca acactgaaag ccgcagggcg gactgcccgg
    14761 cgtcatcgtc gcgggctatg ctgcgcgtcc acgggggaag ccacgcatgt ccggacccag
    14821 caacgccaac gacaccccga tcaccgacta tcggcaactg gtcgaggtca tggcctccgg
    14881 cgagaagccg atcgaggctt ggcggatcgg taccgagcac gagaagttcg gcttccgcct
    14941 cgacgacctg cggccgccga ccttcgatgg cgagcgcggc atcgaggcgc tgctgaaggg
    15001 gctgacccgc ttcggctgga cgccttacga ggaaagcgtg gacgggcagc cggcgcgcgt
    15061 gatcgcgctg accaagggca tggcctcggt gacgctggaa cccgccggcc aactggagct
    15121 ctccggtgcg ccggtcgaaa ccatccacga cacctgcaag gaagtcggca gccacctgcg
    15181 cgaagtgcgc gcggtcgccg acgaattgca gctcggcttc ctcggcatgg gcttccagcc
    15241 gaagtggcgg cgcgacgaga tgccttggat gccgaagggc cgctaccgga tcatgcgcgc
    15301 gtacatgccc aaggtcggca acctgggcct ggacatgatg acccgcacct gcacggtgca
    15361 ggtcaacctc gattacgcct ctgaggcgga catggtgaag aagttccggg tgtcgctggc
    15421 gctgcagccg gtcgccaccg cgttgttcgc cgattcgccc ttcaccgagg gcaagccgaa
    15481 cggccagctc agctatcgct cgcacatctg gaccgacacc gatcccgatc gcacgggcat
    15541 gctggacttc gtgttcgagg acggcttcgg ctacgagcgc tacgtcgatt acctgctcg
//
LOCUS       DAOOCX010000051        15508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3437_length_15508_cov_7.377726, whole genome shotgun sequence.
ACCESSION   DAOOCX010000051 DAOOCX010000000
VERSION     DAOOCX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15508
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3437_length_15508_cov_7.377726"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..846
                     /locus_tag="PK399_06485"
     CDS             88..846
                     /locus_tag="PK399_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPW12799.1"
                     /translation="MPLIETTIHGDIHELRLARPPVNALNPELCAALTQALGDAVAAG
                     AQGIVLAGGPKVFSAGLDVPYLLSLGADQEALLRSWTAFFGAARALAACPVPVVAALA
                     GHSPAGGCVLALCCDYRIMAEGPYRIGLNETQVGLVAPEGIQSLLARMVGPHRAERLL
                     VAGDMVDSSEALRIGLVDELTGIDDVATRARVWLETLLQLPRAPMLRTRAIARAAVID
                     CLRDERIELSKFVSGWMEPDTQAALRAMLARIGK"
     gene            complement(850..1272)
                     /gene="arsC"
                     /locus_tag="PK399_06490"
     CDS             complement(850..1272)
                     /gene="arsC"
                     /locus_tag="PK399_06490"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504567.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HPW12800.1"
                     /translation="MHVELWHNPACGTSRNVLALIRHVGIEPVIVDYLRDPPGIPRLR
                     QAIQEAGLGVRDAIRSKQPEYLALGLDDPGLSDDALLAAMVATPVLINRPFVFTPLGV
                     RLCRPESAMVLDLLPPCDKPFAKEDGDVVIDAAGRRVR"
     gene            complement(1299..2144)
                     /locus_tag="PK399_06495"
     CDS             complement(1299..2144)
                     /locus_tag="PK399_06495"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HPW12801.1"
                     /translation="MAGWTTLVQAETLAVALGRPDLVVLDCRFSLLDPNAGEQAYSRS
                     HLPGAAYAHLERDLSEMGPHGDGRHPWPQAEVLVSKLRRWGIDARSQVIAYDDGDGAY
                     AARLWFLLRMLGHEKVAVLDGGWARWTALGLPVSSSPHQGLPSRYEADFDRSRLVDAR
                     QVEAHLFDGGILVDARAPARFRGEEEPIDRVAGHVPGAVNRPFATNLVDGRFKSPMQL
                     GDEFRALLDGRDPSRVVVMCGSGVTACHHLLAMERAGLKGARLYTGSWSGWIQDPSRP
                     VAKGS"
     gene            complement(2144..2958)
                     /locus_tag="PK399_06500"
                     /pseudo
     CDS             complement(2144..2958)
                     /locus_tag="PK399_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
     gene            complement(3056..3784)
                     /locus_tag="PK399_06505"
     CDS             complement(3056..3784)
                     /locus_tag="PK399_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HPW12802.1"
                     /translation="MKLHVLAASLAALTLTAGIATAQTKPAAPATPAAKPAAPAAAPV
                     VDKTHASYAFGWSLGQDLQNSGEPVDIATVIRGLQDAYGKKTPAYTEQQLGAAYSGFQ
                     QRVQQKMEAQFRKALDDNKAQSANFITQYKAQEGVITLPSGIMYRIAKPGTGAKATAN
                     SQVQIGFRSFLAAVGVPLSGVQTPPAFKVSDAPIPALKETLPLMQQGAVWEVVVPPGK
                     GAGDQNQQFAQQAIAIQVELGSVK"
     gene            3999..4781
                     /locus_tag="PK399_06510"
     CDS             3999..4781
                     /locus_tag="PK399_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009600779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPW12803.1"
                     /translation="MSDSPLLIHDADGIRTITIHRPDKLNALNAATLDALDGAFADAA
                     ADGAIHVVVLTGSGPKAFVAGADIAEMNTLTAVQGRDFSLRGTRMMRRIERMPKPVLA
                     MVNGFALGGGLELAMCCHLRIAADSARVGQPEINLGLIPGFGGTQRLLRLCGRAATLE
                     LCLTGAPINAERALQLGIVNRVVPAADLEAETMKLAAQLAASAPLALRGMLDCVNVGG
                     ECGIEEGLEYEAAQFGLVFSTEDMREGTSAFLGKRKPVFAGK"
     gene            4888..5253
                     /locus_tag="PK399_06515"
     CDS             4888..5253
                     /locus_tag="PK399_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12804.1"
                     /translation="MTCPVCGCTHADPAHAIAQALQADDLDAALQLGLLDAAPCAGCD
                     AACTARLVEVRDARRFALAARERHRARDARLQRRKAEREAARRPKAASDAAQAPALPS
                     AAADALARALAKARERTPR"
     gene            5250..5948
                     /gene="nth"
                     /locus_tag="PK399_06520"
     CDS             5250..5948
                     /gene="nth"
                     /locus_tag="PK399_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPW12805.1"
                     /translation="MTATTQRAARGSKLRPAEIVDLFARLRELDPAPTTELDYTTPFE
                     LLVAVVLSAQATDVGVNKATRKLYPIANTPATILALGEDALKGYINTIGLFNAKAANV
                     IALCRLLLERHDGEVPRTRDALEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRVSNR
                     TGLAPGKDVRTVEDKLVRLVPVEFKRDAHHWLILHGRYTCKARKPDCAHCVIHDLCRW
                     PEKTAPASGDAAWR"
     gene            6054..7283
                     /locus_tag="PK399_06525"
     CDS             6054..7283
                     /locus_tag="PK399_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OprO/OprP family phosphate-selective porin"
                     /protein_id="HPW12806.1"
                     /translation="MKLSRRILSVALLAAIAAPAAHAEVPIDVIAGSEITFEGLLQAD
                     ANWFNSDVANLNGDAQDGRDFDGELRRAELVLKGKGPGNLEWVIGYDAKADKWLDTNL
                     KYKLGGNANHYLQFGQFKQPNSLEELSSTKNNDFISKATITNTWAVARRVGAAYSVGD
                     ANWSITGAYFDDELKRGLAAGAGYGVRGTWAPINATGSILHLGLSYVNYDAELPAVGA
                     NNLVDTQRWRARPQADLSGTRLIDTGNLTLADKVATTGVEAFWVHGPLKLQGEYMRST
                     TSRGGAFSDFTGTGAYVSAVWNVTGETWGYKAGVPTTPLPNEPGRGMLQLALRYDTMD
                     LNDGSLNTSGATPVVTGVLGGEMDAWTVGANWYLRSNFKLAANYVKVSTSKYNSTAKA
                     IVNDDPSIVEFRAQFFW"
     gene            7468..8487
                     /gene="pstS"
                     /locus_tag="PK399_06530"
     CDS             7468..8487
                     /gene="pstS"
                     /locus_tag="PK399_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HPW12807.1"
                     /translation="MIKSIKLRVAALALATTFAAGAMAADVTGAGASFVYPIMSKWSA
                     DYAKATGKKVNYQSIGSGGGIAQIKAGTVDFGSSDAPLKPEELEKFGLAQFPSVIGGV
                     VPVLKVPGVAAGAMKLDGELLADIFLGKVSKWNDPRIAALNGGIKLPDLKITVVHRSD
                     GSGTTFNFVNYLSKVNAEWKSSVGEGTAVKWPVGIGGKGNEGVAAYVKQINGSIGYVE
                     LSYALQQKLAYSRLKNAAGNYVNPSDDSFSAAAANARWADAKDFYLVMTNAPGEASWP
                     ITATNFILMYKTPKNAAGAKNAKDFFKWVYANGDAQAKSLDYVPLPDALVQQIDAYWA
                     AEMKY"
     gene            8650..9759
                     /gene="pstS"
                     /locus_tag="PK399_06535"
     CDS             8650..9759
                     /gene="pstS"
                     /locus_tag="PK399_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HPW12808.1"
                     /translation="MNRQLPRIAALAICLLVGLAACKNESAPSATGATGDAASGAASN
                     AATPAGDAVAAEITGAGASFVYPLISKWSADYAKATGNKVNYQSIGSGGGIAQIKAGT
                     VDFGSSDKPLAPEELAKDGLVQFPSTIGGVVPVVNLPGIEAGKLRLTGTLLADIYLGK
                     VTMWNDAAIAAANPGVALPADKIHVVRRSDGSGTTYNFTNYLAKVSPAWKAAVGEGTT
                     VNWTGSSVGGKGNEGVASYVKQLKGAIGYVELSYALQNRMAHAAMQNAAGAWVQPNAE
                     SFAAAAASADWQNSQDFNLVITDAPGAEAWPITATNFILMRKQPRDAADAKAALEFFR
                     WAYANGTAQAQSLDYVPLPATLVQQVEAYWATQLK"
     gene            9793..10755
                     /gene="pstC"
                     /locus_tag="PK399_06540"
     CDS             9793..10755
                     /gene="pstC"
                     /locus_tag="PK399_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPW12809.1"
                     /translation="MNAIALPVSERDIADTRHDTLFRWLLGATAVFVLVTLAGAALSM
                     LWGGRSVLASEGLDFFFSADWNPVENKYGALVPIYGTVVTAIIAMLIAVPVSFGIAFF
                     LTEVAPRWMRTPVGTAIELLAGVPSIIYGMWGFFVLMPVMREHLIPWLDSHVGTLPVI
                     GKLFQGPPIGAGMLTSGIVLSIMVIPFISSVMREVFLTVPSRLKESAYALGATKWEVS
                     WDIVLPYTRSAVIGGVFLGLGRALGETMAVAFVIGNSVNFTASLLEPSTTIAALIAND
                     FGEATEEYRAALLLLGFVLFIVTFVVLAIARLMLANLSRKEGNR"
     assembly_gap    10927..11026
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11027..11794
                     /gene="pstA"
                     /locus_tag="PK399_06545"
     CDS             <11027..11794
                     /gene="pstA"
                     /locus_tag="PK399_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPW12810.1"
                     /translation="FLGWILWTLLSKGLAHLSLSLFTQDQPPPLEAGGLRNAIVGSLM
                     MCGMGVAIGTPLGIAAGTWLAEVGNHRKLGTVVRFVNDILLSAPSIVLGLFVYAAFVM
                     QTGGNFSAIGGALALAFIVLPVVVRTTDEMLRLVPVQMREAALSLGVPQWKVTMQVLY
                     RSALPGIVTGVLLALARISGETAPLLFTAFGNEFFSLDVTGAMSAVPLVMYKFAGSGY
                     ENWEHLAWAGALLVTLFVLAVSLVARTLLLRNKISND"
     gene            11829..12644
                     /gene="pstB"
                     /locus_tag="PK399_06550"
     CDS             11829..12644
                     /gene="pstB"
                     /locus_tag="PK399_06550"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007966219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPW12811.1"
                     /translation="MNDARLAVAASVRDAILPTDAPVKIAARDLNFWYDRFHAVKHIN
                     LEVPEKRVTALIGPSGCGKSTLLRVFNRIYALYPKLEAKGEVLLDGENILDPKYPMNR
                     LRSKVGMVFQKPVPFPMTIYENVAYGIRHHEKLNKADLDARVELALRQAALWDEVKDK
                     LKESGLGLSGGQQQRLCIARAVALKPDVLLLDEPTSALDPISTSRIEQLVEELKRDFT
                     IMIVTHNMQQAARVSDYTAFMYLGDLVEHGATEQIFSNPVKQQTEDYITGRFG"
     gene            12644..13339
                     /gene="phoU"
                     /locus_tag="PK399_06555"
     CDS             12644..13339
                     /gene="phoU"
                     /locus_tag="PK399_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPW12812.1"
                     /translation="MNNHIVKSYDDEQRVLLDELIRMGHMSVAQLEAALDVVERRDDK
                     AAERVIGNDDAIDALEQQVNHDVIRLIRRGPLAGDLRVILAALRVASDIERIGDLAAN
                     IAKRSMALNLAPPLPHTRGLDALGRMAARQVREVLEAYVQRDGEAALRVRASDAELDT
                     LYTGLFRELLTYMMEDARAITPCTHLLFMAKNLERIGDHATNIAENVWFLIHGEELMP
                     PRDKRDESSTTVV"
     gene            13451..13906
                     /locus_tag="PK399_06560"
     CDS             13451..13906
                     /locus_tag="PK399_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12813.1"
                     /translation="MSQSRKPLVLLVGAAVAAGSLFSMDTLAHGYMQDAKPAAAKAGE
                     GKCGEGKCGEGKCGGQAAGKDAHEGGCGMAKMDTDKDGRLSRAEFDAAHKGKEEKFAS
                     HDLDGDGFISQAEMDKHHADMKAKGDGKATAEGKCGEGKCGEGKCGGNP"
     gene            13911..14735
                     /locus_tag="PK399_06565"
     CDS             13911..14735
                     /locus_tag="PK399_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HPW12814.1"
                     /translation="MADALPLRGAGLGLRRALLDELIEAPVGAFDFLECAPDNWIGVG
                     GVLGEKLQALSSRHPLSCHGLSLSLGGPDPLDRQFLHKTREFLDRHAIALYSEHLSYC
                     SAGGHLYDLLPLPFTDEAVHHVAARIAQVQDVLGRRIAIENVSYYAAPYQALGEIDFI
                     DAVLREADCDLLLDVNNVFVNAVNHGYDARAFIDAMPGERIASLHVAGHYDEADDLKV
                     DTHGTAVKADVWDLLAHAYRAHGLRPTLLERDFNFPPLGTLLDEVQRIRDLQAQAA"
     gene            14732..15484
                     /locus_tag="PK399_06570"
     CDS             14732..15484
                     /locus_tag="PK399_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HPW12815.1"
                     /translation="MNELRQQLDALAAHVRDPDAHPGPPGIEARRLKIYSDLVYNNLD
                     SLLAGNFPVIRKTLGDADWQTLVRGFLSRHHSHTPLFTELGREFIAFLESEAGTDPAR
                     PWLAELAHYEWAELGLQLSEAVLPPHDTNGDLLDGVPMLSPLAWPLAYRWPVHRIGPG
                     FQPIEPPHEPTLVLLRREPDGRVHFSMLSPLLFRLLERVGAERGETGRALLERLAIEA
                     DQSDVDAFIGQAVPMLLRLRDEGVLLGTCRSS"
BASE COUNT         2604 a         5295 c         5132 g         2377 t
ORIGIN      
        1 tgaggagaag gagggcgaac agccatgcgt gcttggtcat gcgcctatga taccggcacc
       61 tcccgatcgc cccatcccgg aacccgcatg ccactgatcg aaaccaccat ccacggtgat
      121 atccacgaac tccgcctggc gcgccccccg gtcaatgcgt tgaatcccga gctgtgcgcg
      181 gcactcacgc aagcgctggg ggatgccgtg gccgcgggtg cgcaaggcat cgtgctggcc
      241 ggtgggccga aggtcttttc cgccggcctg gacgtgccgt acctgctgtc gctcggcgcc
      301 gaccaggagg ccctgttgcg gtcatggacg gcgttcttcg gcgccgcgcg cgcactggcc
      361 gcctgcccgg tgccggtggt ggccgcgctg gccgggcact cgcccgccgg gggctgcgtg
      421 ctggcgctgt gctgcgacta ccggatcatg gccgagggcc cttaccggat cggcttgaac
      481 gaaacccagg tggggttggt ggcgccggaa ggcatccagt cgctgctggc gcggatggtc
      541 ggtccgcatc gcgcggaacg cctgctggtg gccggggaca tggtggattc cagcgaagcc
      601 ctgcgcatcg gcctggtcga cgaactgacc ggcatcgacg acgtcgccac ccgcgcgcgg
      661 gtctggctgg aaacactgct gcaattgccg cgcgcgccga tgctgcggac ccgcgcgatc
      721 gcccgcgccg cggtcatcga ctgcctgcgc gacgaacgca tcgagctgtc gaagttcgtg
      781 tccggctgga tggaacccga cacccaggcc gcgttgcgcg ccatgctggc gcggatcggc
      841 aagtaaccgc tagcggacgc ggcggcccgc ggcgtcgatc accacgtcgc catcctcctt
      901 ggcgaagggt ttgtcgcagg gcggcaagag gtcgagcacc atcgccgact ccggccggca
      961 caggcgcacg ccgagcgggg tgaacacgaa cgggcggttg atcaacaccg gagtggccac
     1021 cattgccgcg agcagggcgt catcggacag gccgggatcg tccagtccaa gcgccaggta
     1081 ttccggctgc ttgctgcgga tcgcatcgcg gacgcccagg ccggcctcct ggatggcctg
     1141 gcgcaggcgg gggatgcctg gcggatcgcg caggtagtcg acgatgaccg gctcgatccc
     1201 gacgtggcgg atcagcgcga gcacattgcg cgaggtgccg caggcggggt tgtgccagag
     1261 ttcgacgtgc atcgcggcaa gccccggggc accggggctc acgacccctt ggcgacagga
     1321 cgcgagggat cctggatcca gccgctccac gacccggtgt agaggcgcgc gcccttcagg
     1381 ccggcgcgtt ccatcgccag caggtggtgg caggccgtca cgccggaacc gcacatcacc
     1441 accacccgcg acggatcgcg gccgtcgagc agtgcgcgga actcgtcgcc cagttgcatc
     1501 ggcgacttga agcggccatc gacgagattg gtcgcgaagg gccggttgac cgccccgggc
     1561 acgtggccgg cgacgcggtc gatcggctct tcctcgccac ggaagcgcgc cggtgcgcgc
     1621 gcgtcgacca agatgccgcc atcgaacagg tgcgcttcga cctgtcgcgc atcgaccagg
     1681 cgcgaacggt cgaagtccgc ttcgtaacgc gagggaaggc cctggtgcgg cgagctggaa
     1741 accggcaggc ccagcgcggt ccagcgcgcc caaccgccgt ccagcacggc gaccttttca
     1801 tggccgagca tgcgcagcag gaaccacagg cgagcggcgt aggcgccgtc gccatcgtcg
     1861 taggcaatga cctgcgagcg cgcatcgatg ccccagcggc gcagcttgga caccagcacc
     1921 tcggcctgcg gccagggatg gcggccgtcg ccgtgcggtc ccatctcgga gaggtcgcgc
     1981 tccaggtgcg cgtacgcggc ccccggcagg tgcgagcgcg aataggcctg ctcgcccgca
     2041 ttcggatcga gcaaggaaaa gcggcagtcc agcaccacca ggtcggggcg gccgagcgcc
     2101 accgccagtg tttcggcctg caccagggtg gtccatccgg ccatcaggtt ctcccggtgg
     2161 gcgcgggcgc caggcgctcg cggaggttga acaggatcga ggcggtcacg ccccagatcc
     2221 gttgcggttg gtagcggtat tggaacacgt ggcgcgcccg cccgccgaaa tgcaggggaa
     2281 tccgttccag ttgacgggga tccagcagca actccagcgg cacttcgaag acctccgcga
     2341 cctcggaggg atcgggcctg gccacgaagt ccggtgccac cagcaccagc gccggctgca
     2401 cgcggaaccc ggtgatcgta agcagcgggt ccaggtaacc cagcggccgc gcctgggagg
     2461 cacgcaggcc gacttcttcg gcagcctcgc gcagggccgc gtccagtggc gttgcatcgc
     2521 ccgactccat gcgccccccg gggaaactga cctggccgcc atggtggcgc agcgcgtcgt
     2581 tgcgccgcgt gagcaacacg ctggcacctt catcccgcgg aatcacgccg accagcaccg
     2641 cggccgcctg gggctcgccc ggcgacaaca gcccctcgag ttcgaacaga ttccacggct
     2701 cgcccaccgg gggtgacgcc agtggatgca gggcgccacg cagccgcgcc gcgagcgcct
     2761 cggggttcat gacgcctcgc gcgccagtgc ccggcgcggc aggacgtcgt ccatcatccg
     2821 caagcggaca tcgtcgccca tcatcggcca cgcggcgatc tcgttgccgg tgcgatggca
     2881 gcccacgcac aggccttcgt cgtcgatcgt gcagacgccg atgcaggggc tgaggacggc
     2941 gcggtactgg aacgtcatgc ggtgtggacc ctggcgtggc ctgggagagc ggccatccct
     3001 tgaaacgcga tgcgccggca ggaccggcgc atcgggtgga gcatgctgca atcgattact
     3061 tgacgctgcc cagttcgacc tggatggcga tcgcctgctg cgcaaactgc tggttctggt
     3121 cgccggcacc cttgcccggc ggcaccacca cttcccacac ggcgccctgc tgcatcagcg
     3181 gcagggtttc cttcagcgcc gggatcgggg catcggagac cttgaacgcc ggcggcgtct
     3241 gcacgccgct gagcggcacg ccgaccgcgg ccaggaagct gcggaagccg atctgcacct
     3301 ggctgttggc ggtcgccttg gcgccggtac cgggcttggc gatgcggtac atgatgccgc
     3361 tgggcagggt gatcacccct tcctgcgcct tgtactgggt gatgaagttg gcggactgcg
     3421 ccttgttgtc gtccagcgcc ttgcggaact gcgcttccat cttctgctgc acgcgctgct
     3481 ggaagccgga ataggccgcg cccagttgct gttcggtgta ggcgggggtc ttcttgccgt
     3541 aggcgtcctg caggccgcgg atcacggtgg cgatgtccac cggctcgccg gagttctgca
     3601 ggtcctggcc cagcgaccag ccgaaggcat agctggcgtg agtcttgtcg accaccggcg
     3661 ccgccgcggg agcggccggc ttggcggcgg gggtggccgg agcggccggc ttggtctgag
     3721 cggtggcgat gccggcggtc agggtcaggg ccgccaggct ggcagcaagc acatgcaact
     3781 tcatccacaa cctcgtcgtt tgtaaggcac cgccgaacgg cgggaccggg ctccggcatg
     3841 tcgccggaag gagtgtctgg cgccggtcga accgctccgg catgacacgc taggataccg
     3901 gccatggcac ttaaccgcca ctgaggccag ccactccgac acccgcgcgt ggcggaagtt
     3961 ccggcccgcc ctttcccatt catgtccaga gggaccgcat gtccgattcc ccgttgctga
     4021 tccacgatgc cgacggcatc cgcacgatca ccatccaccg ccccgacaag ctcaatgccc
     4081 tcaacgccgc caccctggat gcgctcgatg gcgctttcgc cgacgctgct gccgatggcg
     4141 cgatccacgt ggtggtgctg accggcagtg ggcccaaggc cttcgtcgcc ggtgccgata
     4201 tcgccgagat gaacaccctc accgcggtgc agggccgcga tttctcgctg cgcggcacgc
     4261 ggatgatgcg gcggatcgag cgcatgccga agccggtgct cgccatggtc aacggcttcg
     4321 cccttggcgg cgggctggaa ctggcgatgt gctgccacct gcgcatcgcc gcggacagcg
     4381 ccagggtcgg gcaaccggaa atcaacctgg gcctgatccc gggcttcggc ggtacccagc
     4441 gcctgctgcg cctgtgcggg cgtgcggcca cgctggagct gtgcctgacc ggcgcgccga
     4501 tcaatgccga acgcgcgctg cagctcggca tcgtcaaccg ggtggtgccg gcagcggatc
     4561 tggaagccga gacgatgaag ctggccgcgc agctggcggc atcggcgccg ctggccctgc
     4621 gcggcatgct ggactgcgtg aacgtgggcg gcgaatgcgg gatcgaggaa ggcctggagt
     4681 acgaggcggc gcaatttggc ctggtgttct cgaccgagga catgcgggag ggcacctccg
     4741 cgttcctggg caagcgcaag ccggtgttcg cggggaaatg accgccctgt cgtcctgatc
     4801 gcagcgaagg acctgcttca tcgacaagca gacccctcgc tgcgctcggg atggcaaagg
     4861 atttcgtcca tggcctccga taccctcatg acctgtcctg tttgcggttg cacgcatgcg
     4921 gatccggcgc acgccatcgc acaggcgctg caggcggatg acctcgatgc cgcgctgcaa
     4981 ctcggcttgc tggatgccgc tccctgcgcc ggctgcgatg ccgcctgcac ggcacgcctg
     5041 gtcgaggtgc gcgatgcgcg ccgcttcgcg ctggccgcgc gcgaacgcca tcgcgcacgt
     5101 gatgcacgcc tgcagcggcg caaggccgaa cgcgaggccg cgcgtcgccc caaggcagcc
     5161 agcgacgcgg cccaggcgcc cgcgttgccg tcggccgccg cggatgcgct ggcgcgtgcg
     5221 ctggcgaagg cgcgggaacg gacaccgcga tgacggcgac cacgcaacgc gcggcacgcg
     5281 gcagcaagct gcgcccggcc gaaatcgtgg acctgttcgc gcgcctgcgc gaactcgatc
     5341 ccgcgccgac caccgaactc gactacacca cgccattcga attgctcgtt gcggtcgtac
     5401 tgtccgcgca ggcgaccgat gtcggcgtca acaaggccac gcgcaagctg taccccatcg
     5461 ccaatacgcc ggcgacgatc ctggcgctcg gcgaggacgc gctgaagggt tacatcaaca
     5521 ccatcggcct gttcaatgcg aaggccgcga acgtcatcgc gctgtgccgc ctgctgctgg
     5581 aacgccacga tggcgaagtc ccgcgcacgc gcgacgcgct cgaggcgctg ccgggcgtgg
     5641 gacgcaagac cgccaacgtg gtcctcaaca ccgcgttcgg cgagcccacc atcgcggtgg
     5701 acacgcacat cttccgcgtg tccaaccgca ccggcctggc ccccggcaag gatgtgcgca
     5761 cggtcgagga caagctggtc cggctggtcc ccgtcgaatt caagcgcgac gcccaccact
     5821 ggctgatcct gcacggacgc tacacctgca aggcacgcaa gccggattgc gcgcactgcg
     5881 tgatccacga cctctgccga tggcccgaga aaaccgcgcc agccagcggc gatgccgcat
     5941 ggcggtgaca tgtcgataaa aagacaggcc tgtcacaata acgaaagatt cacgccctag
     6001 gctgcgcgca atcggcaccc gctgccgctt cccaacctga ccagggcatc cccatgaaac
     6061 tctcccgccg catcctgtcc gtggccctgc tggccgccat cgccgcgccc gccgcccacg
     6121 cggaggtgcc gatcgacgtc atcgccggct ccgaaatcac cttcgagggc ctgctgcagg
     6181 ccgatgccaa ctggttcaac agcgacgtcg ccaacctcaa cggcgacgcc caggacgggc
     6241 gcgatttcga cggcgaactg cgccgcgccg agctggtgct gaagggcaag ggcccgggca
     6301 acctcgagtg ggtgatcggc tacgacgcca aggccgacaa gtggctggac accaacctga
     6361 agtacaagct cggcggcaac gccaaccact acctgcaatt cggccagttc aagcagccga
     6421 acagcctgga agaactgagc tcgaccaaga acaacgactt catctcgaag gcgaccatca
     6481 ccaacacctg ggcggtggcc cgccgcgtgg gcgccgccta cagcgtgggc gacgccaact
     6541 ggagcatcac cggcgcgtat ttcgacgacg agctgaagcg cggcctcgcg gctggcgcgg
     6601 gctatggcgt gcgcggcacc tgggcaccga tcaacgccac cggcagcatc ctgcacctgg
     6661 gcctgtcgta cgtgaactac gacgccgaac tgccggccgt tggcgccaac aacctggtcg
     6721 acacccagcg ctggcgtgca cgcccgcagg ccgacctgtc cggcacgcgg ctgatcgata
     6781 ccggcaacct gaccctggcc gacaaggtcg cgacgaccgg cgtcgaggcg ttctgggtgc
     6841 atggcccgct caagctgcag ggcgaataca tgcgcagcac caccagccgc ggcggcgcgt
     6901 tctccgactt caccggcacc ggcgcctacg tcagcgcggt gtggaacgtc accggcgaga
     6961 cctggggcta caaggccggc gtgccgacca ccccgctgcc caacgaaccg ggacgcggca
     7021 tgctccagct ggccctgcgc tacgacacca tggacctcaa cgacggcagc ttgaacacca
     7081 gcggcgccac cccggtcgtc accggcgtac tgggcggcga aatggatgcc tggaccgtgg
     7141 gcgccaactg gtacctgcgc tccaacttca agctggccgc gaactatgtg aaggtcagca
     7201 ccagcaagta caacagcacg gccaaggcga tcgtcaacga cgatcccagc atcgtcgagt
     7261 tccgcgcgca gttcttctgg taaggcctgc aggcgatcca tcgagaaggg cgtggcgaga
     7321 gccgcgccct tttcctttgt cgtcgcgacg acgctgtcat cgggctgtca cgaaaccacc
     7381 atcggcctgt catccggccg ttatgaaatg cgcgcagccg ggcaatgccc gccctttccc
     7441 attcgcgcac cactcctgga gcatcacgtg atcaagtcca tcaagttacg cgtcgccgcg
     7501 ctcgcgctgg cgaccacgtt cgccgccggc gccatggccg cagacgtcac cggcgccggc
     7561 gcctcgttcg tctatccgat catgtcgaaa tggtccgccg actacgcgaa ggcgaccggc
     7621 aagaaggtca actaccagtc gatcggctcc ggcggcggca tcgcccagat caaggcaggc
     7681 acggtggact tcggctcctc cgatgccccg ctgaagccgg aagaactgga aaagttcggc
     7741 ctggcgcagt tcccgtcggt gatcggcggc gtggtgccgg tgctcaaggt gccgggcgtg
     7801 gccgcgggcg cgatgaagct ggacggcgaa ctgctggccg acatcttcct gggcaaggtc
     7861 agcaagtgga acgacccgcg catcgccgcc ctgaatggcg ggatcaagct gcccgacctg
     7921 aagatcaccg tggtgcaccg ttcggacggc tccggcacca ccttcaactt cgtcaactac
     7981 ctgtcgaagg tcaatgccga atggaagtcc agcgtgggcg aaggcaccgc ggtgaagtgg
     8041 ccggtcggca tcggcggcaa gggcaacgag ggcgtggccg cctacgtcaa gcagatcaac
     8101 ggctcgatcg gctatgtcga gctgtcctac gcactgcagc agaagctggc gtacagccgg
     8161 ctgaagaacg ccgccggcaa ctacgtgaac ccgagcgacg acagcttctc cgctgccgcc
     8221 gcgaacgccc ggtgggccga tgccaaggac ttctacctgg tcatgaccaa tgccccgggc
     8281 gaggccagct ggccgatcac cgccaccaat ttcatcctga tgtacaagac cccgaagaac
     8341 gcggctggcg cgaagaacgc caaggacttc ttcaagtggg tgtatgccaa cggcgatgcc
     8401 caggccaagt cgctcgacta cgtgccgctg ccggacgcgc tggtgcagca gatcgacgcc
     8461 tattgggcgg cagagatgaa gtactgatcg atccgcgcaa ctccaacgcg cgaatcaacg
     8521 ggcgggcctt cgggcccgcc tttttcgtgg tcgatgcccg cgccgggacg aaaacacggg
     8581 cgctgtcatc gacgtgtcac aaaaacatca tttcatgtcg cgatccgaac gagcatcgaa
     8641 gagacccgca tgaaccgtca actgccccgc atcgccgccc tcgccatctg tctgctggtc
     8701 ggccttgccg cctgcaagaa cgagtctgcg cccagcgcca ccggcgccac cggcgatgcc
     8761 gcgtcgggcg cggcatcgaa cgcggccacc cccgcgggcg atgcggtcgc cgccgagatc
     8821 accggcgccg gcgcgtcgtt cgtgtatccg ctgatctcca agtggtccgc cgactacgcc
     8881 aaggccaccg gcaacaaggt caactaccag tcgatcggtt ccggcggcgg catcgcccag
     8941 atcaaggccg gcaccgtgga cttcggttcc tccgacaagc cgctggcgcc ggaagaactc
     9001 gcgaaggacg gcctggtgca gttcccgtcg acgatcggcg gcgtggtgcc ggtggtgaac
     9061 ctgcccggca tcgaggccgg caagctgcgc ctcaccggca cgctgctcgc ggacatctac
     9121 ctgggcaagg tcacgatgtg gaacgacgcc gcgatcgccg ccgccaaccc cggcgtggcg
     9181 ctgccggcgg acaagatcca cgtggtgcgc cgctccgacg gctcgggtac cacctacaac
     9241 ttcaccaact acctggccaa ggtcagcccg gcgtggaagg ccgcggtcgg cgaaggcacc
     9301 acggtcaact ggaccggcag cagcgtgggc ggaaagggca acgaaggcgt ggcctcctac
     9361 gtcaagcagc tgaagggcgc gatcggctac gtcgagctgt cgtacgcgct gcagaacagg
     9421 atggcgcatg cggcgatgca gaacgccgcg ggcgcatggg tgcagccgaa tgccgaatcg
     9481 tttgccgctg ccgccgccag cgccgactgg cagaactcgc aggacttcaa cctggtgatc
     9541 accgacgccc ccggcgccga ggcctggccg atcaccgcca ccaacttcat cctgatgcgc
     9601 aagcagccgc gcgatgccgc ggacgccaag gccgcgctgg aattcttccg ctgggcctat
     9661 gccaacggca ccgcccaggc gcagtcgctg gactacgtgc cgctgcccgc aacgctggtg
     9721 cagcaggtcg aggcgtactg ggcgacgcaa ctgaaataag cgcgtcccgt catcgtccac
     9781 ccgagaacac gcatgaacgc catcgccctc cccgtgtcgg aacgcgacat cgccgacacg
     9841 cgccatgaca ccttgttccg ctggttgctg ggtgcgaccg cggtgttcgt gctggtcacg
     9901 ctggcaggtg ccgcactgtc gatgttgtgg ggcggccgca gtgtgctggc cagcgaaggc
     9961 ctggacttct tcttctccgc ggactggaac ccggtcgaga acaagtacgg ggccctggtg
    10021 ccgatctacg gcaccgtggt caccgcgatc atcgccatgc tgatcgcggt gccggtgagc
    10081 ttcggcatcg cgttcttcct gaccgaggtc gcgccgcgct ggatgcgcac gccggtgggc
    10141 acggccatcg aactgctggc cggcgtcccc tcgatcatct acggcatgtg gggcttcttc
    10201 gtgctgatgc cggtgatgcg cgagcacctg atcccgtggc tcgacagcca cgtcggcacc
    10261 ctgcccgtca tcggcaagct cttccagggg ccgccgatcg gcgccggcat gctgacgtcc
    10321 ggcatcgtgc tttcgatcat ggtgatcccg ttcatctcct cggtgatgcg cgaggtgttc
    10381 ctgaccgtgc catcgcgcct gaaggaatcg gcctacgcac tgggcgcgac caagtgggaa
    10441 gtgagctggg acatcgtcct gccgtacacg cgctcggcgg tgatcggcgg cgtgttcctg
    10501 ggcctgggcc gcgcccttgg cgaaacgatg gcggtcgcct tcgtgatcgg caacagcgtc
    10561 aacttcaccg cgtcgttgct ggaaccgtcg accaccatcg cggcgctgat cgccaacgat
    10621 ttcggcgaag ccaccgagga atatcgcgcg gcgctgctgc tgctcggctt cgtgttgttc
    10681 atcgtcacct tcgtggtgct ggcgatcgcc cgcctgatgc tggccaacct gtcgcgcaag
    10741 gagggcaacc ggtgaacgcg atcgtcatga cccctcgcga catcgccgcg gccaggctgt
    10801 atcgccgtcg ccgcctggtc aacatcctcg ccatcggcct ggcgtgtgcc gccgccgtgt
    10861 tcggcctggc attcctcggc tggatcctgt ggacgctgtt gtccaagggc ctggcccacc
    10921 tgagccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttcc tcggctggat
    11041 cctgtggacg ctgttgtcca agggcctggc ccacctgagc ctgtcgctgt tcacccagga
    11101 ccagccgcca ccgctcgaag cgggcggcct gcgcaatgcc atcgtcggca gcctgatgat
    11161 gtgcggcatg ggcgtggcga tcggcacgcc gctcggcatc gccgccggca cctggctggc
    11221 ggaagtcggc aaccatcgca agctcggcac ggtggtgcgc ttcgtcaacg acatcctgct
    11281 gtcggcgccg tcgatcgtgc tgggcctgtt cgtgtatgcg gccttcgtga tgcagaccgg
    11341 cggcaacttc tccgccatcg gcggtgcgct ggcactggcc ttcatcgtgc tgccggtggt
    11401 ggtgcgcacc accgacgaga tgctgcggct ggttccggtg cagatgcgcg aagcggcgtt
    11461 gtcgctgggc gtgccgcagt ggaaggtcac gatgcaggtg ctgtaccgca gcgcgctgcc
    11521 cggcatcgtc actggcgtcc tgctcgccct tgcgcgcatt tccggcgaga ccgccccgct
    11581 gctgttcact gccttcggca acgagttctt cagcctggac gtcaccggcg cgatgagcgc
    11641 ggtgccgctg gtcatgtaca agttcgccgg ttccggctac gagaactggg aacacctggc
    11701 ctgggccggc gcgctgctgg tgaccctgtt cgtgctcgcg gtgagcctgg tcgcgcgcac
    11761 cctcctgctg cgcaacaaga tttcgaatga ctgacgcgcc aatgacccat ctcacgcgga
    11821 tcgatgccat gaacgatgca cgccttgccg tcgccgcctc cgtgcgcgat gccatcctgc
    11881 ctaccgacgc gccggtgaag atcgccgcgc gcgacctgaa cttctggtac gaccggttcc
    11941 acgcggtcaa gcacatcaac ctggaggtcc cggagaagcg cgtgaccgcg ctgatcggcc
    12001 cgtccggctg cggcaagtcg acgctgctgc gcgtgttcaa ccgcatctat gcgctgtacc
    12061 cgaagctgga agcgaagggc gaggtgctgc tggacggcga gaacatcctc gacccgaagt
    12121 acccgatgaa ccgcctgcgc agcaaggtcg gcatggtgtt ccagaagccg gtgccgttcc
    12181 cgatgacgat ctacgagaac gtggcctacg gcatccgcca ccacgagaag ctcaacaagg
    12241 ccgacctcga cgcgcgcgtg gaactggcgc tgcggcaggc cgcgctgtgg gacgaggtca
    12301 aggacaagct gaaggaaagc ggcctgggcc tgtccggcgg ccagcagcaa cgcctgtgca
    12361 tcgcccgcgc ggtggcgttg aagcccgacg tgctgttgct ggacgaaccg acctcggcgc
    12421 tcgacccgat ctccaccagc cgcatcgagc aactggtcga ggaactcaag cgcgacttca
    12481 ccatcatgat cgtgacccac aacatgcagc aggccgcgcg cgtgtccgac tacaccgcct
    12541 tcatgtacct gggcgacctg gtcgagcacg gagcgaccga gcagatcttc tccaacccgg
    12601 tcaagcaaca gaccgaggac tacatcaccg ggaggttcgg ctgatgaaca accatatcgt
    12661 caagagctac gacgacgagc agcgcgtgct gctggacgag ctgatccgca tgggccacat
    12721 gtcggtggcg caactggagg ccgcgctgga cgtggtcgag cgccgtgacg acaaggccgc
    12781 ggaacgggtg atcggcaacg atgacgcgat cgacgcactg gaacagcagg tcaaccacga
    12841 cgtgatccgg ctgatccgcc gcggcccact ggccggcgac ctgcgggtga tcctggcggc
    12901 gctgcgcgtg gcgtcggaca tcgaacgcat cggcgacctg gcggccaata tcgccaagcg
    12961 ttcgatggcg ctgaacctgg ccccgccgct gccgcacacg cgcgggctcg acgcactcgg
    13021 tcgcatggcc gcgcgccagg tgcgcgaggt gctggaggcc tacgtgcagc gcgacggcga
    13081 ggctgccctg cgggtccgcg ccagcgacgc cgaactggac acgctgtaca ccggcctgtt
    13141 ccgcgaactg ctgacctaca tgatggaaga cgcgcgcgcg atcacccctt gcactcacct
    13201 gctgttcatg gcgaagaacc tggagcggat cggcgaccac gccaccaaca tcgccgagaa
    13261 cgtctggttc ctgatccacg gcgaggaatt gatgccgccg cgcgacaagc gcgacgaaag
    13321 cagcaccacc gtggtctgaa ggccacatcc accgggatga atgcgccgcg gccggcatga
    13381 tgctggccgc cgcgcccagc cggcggtagc atcggtgctc ccgtaccgcg cgtcgcgcaa
    13441 ggagccaccc atgtcgcaat cccgcaaacc cctggtgctg ctggtcggtg ccgccgtcgc
    13501 cgccggttcg ctgttctcga tggacaccct ggcgcatggt tacatgcagg atgccaagcc
    13561 cgctgccgcg aaggccggcg aaggcaagtg cggggaaggc aaatgcggcg agggcaagtg
    13621 cggtggccag gctgccggca aggacgccca cgaaggcggt tgcggcatgg cgaagatgga
    13681 caccgacaag gacggccggc tgtcgcgcgc cgaattcgat gccgcgcaca agggcaagga
    13741 agagaagttc gccagccatg acctggacgg cgacggcttc atcagccagg ccgagatgga
    13801 caagcaccac gccgacatga aggccaaggg tgacggcaag gccacggccg aaggcaagtg
    13861 tggcgaaggc aaatgcggcg agggcaagtg cggcggcaat ccctgatcgc atggcggatg
    13921 cactgccgtt gcgcggtgcc ggcctcggcc tgcgccgggc gctgctggac gaactgatcg
    13981 aggcgcccgt gggcgccttc gacttcctgg aatgcgcgcc ggataactgg atcggcgtgg
    14041 gtggcgtcct cggcgagaag ctgcaggcgc tgtcgtcgcg gcatccgctg agttgccacg
    14101 gcttgtcgct gtcgctgggc ggaccggatc cgctcgacag gcagttcctg cacaagaccc
    14161 gcgagttcct cgatcgccac gcgatcgcgc tgtacagcga acacctgagt tactgcagcg
    14221 cgggcggcca tctctacgac ctgctgccgc tcccgttcac cgacgaagcc gtgcatcacg
    14281 tcgctgcccg gatcgcccag gtgcaggatg tactgggccg gcgcatcgcc atcgagaacg
    14341 tcagctacta cgccgcaccg taccaggcgc tgggcgagat cgacttcatc gatgccgtcc
    14401 tgcgcgaggc cgattgcgac ctgctgctgg acgtcaacaa cgtcttcgtc aacgccgtca
    14461 accacggcta cgacgcgcgc gccttcatcg acgcgatgcc cggcgagcgc atcgccagcc
    14521 tccatgtcgc cgggcattac gacgaagccg acgacctgaa ggtcgacacc cacggcaccg
    14581 cggtgaaggc ggacgtatgg gacctgctgg cgcatgcgta tcgcgcgcac ggcctgcgcc
    14641 ctaccctgct ggaacgcgat ttcaatttcc cgccgctggg cacgctgctg gacgaggtgc
    14701 agcgcatccg agacctccag gcgcaggccg catgaacgaa ctgcgtcagc aactggacgc
    14761 actcgccgcg catgtgcgcg acccggacgc gcatcccggc ccgcccggca tcgaagcgcg
    14821 gcggctgaag atctacagcg acctggtcta caacaacctg gatagcctgc tggccggcaa
    14881 tttcccggtc atccgcaaga ctttgggcga tgccgactgg caaacgctgg tgcgcggttt
    14941 cctgtcgagg caccacagcc atacaccgct gttcaccgaa ctcggccgcg aattcattgc
    15001 gttcctggaa tccgaagccg gcaccgaccc ggcacgcccc tggctagccg aactcgcgca
    15061 ctacgaatgg gccgaactcg gcctgcaact cagcgaagcc gtgcttcccc cgcacgacac
    15121 gaacggcgac ctgctcgacg gcgtgcccat gctgtcgccg ctggcatggc cgcttgcgta
    15181 ccggtggccc gtgcaccgga tcgggccggg cttccaaccc atcgagccgc cgcacgaacc
    15241 cacgctggtg ctcctacgcc gcgagcccga cggtcgcgtg catttctcga tgctgtcgcc
    15301 gttgctgttc cgcttgctgg agcgggtcgg tgccgagcgg ggcgagacgg gtcgcgcgtt
    15361 gctggaacga cttgcgattg aagccgatca gtccgatgtc gatgcgttca tcgggcaggc
    15421 agtgccgatg ttgctgcggc tgcgcgatga aggtgtgttg ttgggtacgt gtcgatcttc
    15481 gtaattcggg catggtggct gcttccgg
//
LOCUS       DAOOCX010000052        15258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3511_length_15258_cov_9.579162, whole genome shotgun sequence.
ACCESSION   DAOOCX010000052 DAOOCX010000000
VERSION     DAOOCX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15258
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3511_length_15258_cov_9.579162"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PK399_06575"
     CDS             complement(<1..601)
                     /locus_tag="PK399_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW12816.1"
                     /translation="MRILLVEDEAPLRETLAARLKREGYAVDAAQDGEEGLYMGREVP
                     FDVGIIDLGLPKMSGMELIKALRDEGKKFPVLILTARSSWQDKVDGLKQGADDYLVKP
                     FHVEELLARINALVRRAAGWSKPTLECGPVMLDLAAQTVSVNGGNVDLTSYEYKVLEY
                     LMMHAGELVSKADLTEHIYQQDFDRDSNVLEVFIGRLRKK"
     gene            complement(669..1094)
                     /locus_tag="PK399_06580"
     CDS             complement(669..1094)
                     /locus_tag="PK399_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12817.1"
                     /translation="MSPPAMYRVPKPLSKLLCVVFLAGLPAASAWAQQAPPPPPRDRG
                     DARGNDDNRRGSLSDAVRRVERDTRGEVLSAERVQYDGRDMHRVKVVDDQGRVRVFMQ
                     DPQRRDGGAPPRSGGNNDGGGNNDRGQAPRRPPARDDDD"
     gene            complement(1214..2125)
                     /locus_tag="PK399_06585"
     CDS             complement(1214..2125)
                     /locus_tag="PK399_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HPW12818.1"
                     /translation="MITRDIDAFLAFARDAAPDFGPATARAAFLVAPDGFALAEQSAQ
                     DNAYMADAAAFDAQRASMQHRNLHRAISTVLPTVCFAGDPATPDALFPNNVFGTAAGR
                     CIIGRMRHPVRQREAIRSDIRGFFAEMLGRDEVDLSVQPHPCELTGALVIDRASGLGF
                     CGLSERCDQAGARLMHDAFGLRATLLFDLAPGEYHTNVVLAVLAGRAAVVCPRGFADP
                     AVPAAIAGLYAPHAVVCDDAEHAAFVGNCIALSADTVWMSANAGAALSATHRQALADA
                     GFRVETVVLDAIEAAGGSLRCCIGEVY"
     gene            complement(2122..3123)
                     /gene="dusA"
                     /locus_tag="PK399_06590"
     CDS             complement(2122..3123)
                     /gene="dusA"
                     /locus_tag="PK399_06590"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HPW12819.1"
                     /translation="MTAAPAIPLHQLRLSVAPMMDWTDAHCRAFHRVLAPHARLYSEM
                     VHANAVIHGDRARLLAMDPCEHPVALQLGGSEPALLAQAARIGVDAGFDEINLNCGCP
                     SDRVQAGRFGACLMREPALVADSVAAMIAAVPGVPVTVKCRLGVDDDHEWARFLAFID
                     TIAAAGCVTFVVHARNAWLQGLSPKENREVPPLRYDWAYRLKQERPTLQVVVNGGIAD
                     AAEATAHLGHVDGAMLGRAAYHTPYLLHELDVAWFGGAVVSRSELLRAYRPYVQAQLA
                     RGVLLKHIVRHVLGLFAGQPGGRAFRQVLSEGAHKPGTDWKLVEQALALTERRERVA"
     gene            3216..5825
                     /gene="acnD"
                     /locus_tag="PK399_06595"
     CDS             3216..5825
                     /gene="acnD"
                     /locus_tag="PK399_06595"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014089552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HPW12820.1"
                     /translation="MNVDFRKPLPGTPLDWYDARAAVDALRPGAYAALSYTARVHAEN
                     IVRKADPAMRDAYLLQLIERRRDLDFPWFPARVVCHDILGQTALVDLAGLRDAIAEQG
                     GDPAQVNPVVPVQLIVDHSLAVECGGYDPQAFEKNRAIEDRRNADRFHFIDWTKQAFE
                     NVDVIPPGNGIMHQINLEKMSPVIYTQDGVAFPDTCVGTDSHTPHVDALGVIAIGVGG
                     LEAENVMLGRASWMRTPEIVAVELTGKPQAGITATDVVLALTEFLRKQKVVGAYLEFR
                     GEGAAALTIGDRATISNMAPEYGATAAMFFIDGNTLDYLRLTGREEQQVQLVELYAKA
                     AGLWADDLAAAQYERTLHFDLSSVVRNMAGPSNPHKRLPTSDLAERGIAGNLEGALKQ
                     EAQGLMPDGAVIIAAITSCTNTSNPRNVIAAALLARNANARGLTRKPWVKTSLAPGSK
                     AVQLYLEDAGLLSELEQLGFGIVAFACTTCNGMSGALDPSIQQEIIERDLYATAVLSG
                     NRNFDGRIHPYAKQAFLASPPLVVAYAIAGTVRFDIEQDVLGLGHDGKPVTLKDIWPS
                     DAEIDAVVKASVKPEQFRKVYGPMFNVRVEHGSPVSPLYDWRPKSTYIRRPPYWEGAL
                     AGARTLRGMRPLAVLPDNITTDHLSPSNAILLDSAAGEYLARMDVPEEDFNSYATHRG
                     DHLTAQRATFANPKLINEMAVVDGMVQQGSLARVEPDGKVMRMWEAIETYMDRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLVGMGVLPLEFRNGDTRH
                     TYAIDGTETFDVVGDRTPGATLTLVIHRMNGERIEVPVTCRLDSDEDVEVYEAGGVLQ
                     RFAQDFLAANKAA"
     gene            5827..7041
                     /gene="prpF"
                     /locus_tag="PK399_06600"
     CDS             5827..7041
                     /gene="prpF"
                     /locus_tag="PK399_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HPW12821.1"
                     /translation="MTTSHSPRHAPQLRVPATYMRGGTSKGVFFTLDDLPDFARAPGK
                     PRDALLLRVIGSPDPYGKQTDGMGGATSSTSKVVIIAKSAVPGHDVDYLYGQVPIEGD
                     FIDWSGNCGNLSSAVGPFAIANGLVDADRVPRTGIATVRIWQANIGKTIVAHVPMTEG
                     EVQETGDFELDGVTFPAAEIALEFLDPADEGEDGGAMFPTGNLTDELDVPGVGAFRAT
                     LINAGVPMVFVDAAALGFDGTELQPAINADKAMLARLEAIRAVGAVRMGLIHDVSEAA
                     ARPLTPKIAFVAPPKDYVSSSGKGIDATGIDLNARAISMGKLHHAMMGTASVAIATAA
                     AVPGTVVNTAAGGGSRGAVRFGHPSGTMRVGADVAQVDGQWVATKAVMSRSARVLMEG
                     VVRVPRGQVAAD"
     gene            complement(7038..7940)
                     /locus_tag="PK399_06605"
     CDS             complement(7038..7940)
                     /locus_tag="PK399_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW12822.1"
                     /translation="MSTCEHRIDLAGRVAIVTGAGGGLGRQHALLLARRGARVVVNDL
                     GDAAQAVAAEIVAAGGQARALQCSVTDADAVQAMVDAVLAEWGRVDILVNNAGILRDK
                     SFAKMPLDDFRAVVDVHLMGAANCTKAVWDAMRAQAYGRIVMTTSSSGLYGNFGQANY
                     AAAKMALVGLMQTLALEGAKHGIKVNCLAPTAYTRMMEGILPQDQLDMLDPALVSPAV
                     LALVADGAPTRAILCAGAGGYELAHITLTEGIHLGTTPTAEALLERWGELAERDGEMV
                     PGTGFDQARHELRKAGFAFDEAAG"
     gene            complement(7937..8347)
                     /locus_tag="PK399_06610"
     CDS             complement(7937..8347)
                     /locus_tag="PK399_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HPW12823.1"
                     /translation="MTDLQEPSLIVHRGGCHCGRVRFEVDAPANPDVLDCNCSICRMT
                     GFLHVIVPASRFRLLSGADDLAEYTFNTGAAKHRFCRHCGIKGFYVPRSHPDGFSVNA
                     RCLDDIDLAALNIIPFDDRDRDAAMAAIAHLERP"
     gene            complement(8382..8828)
                     /locus_tag="PK399_06615"
     CDS             complement(8382..8828)
                     /locus_tag="PK399_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HPW12824.1"
                     /translation="MSRRSPPLLLCLLLLVGCDAAAPRAPATGRRLPAIDPGAVDSAL
                     EFRGERPCVDCAGIDSWLRLQSGAGLQRYQLVERYRGEGRDLRFEDEGDWTARGDLLR
                     LRSAQGGERVYLRQDDGSLQARGTDGALLPALADDVLLPTSFEGDR"
     gene            complement(8862..9590)
                     /locus_tag="PK399_06620"
     CDS             complement(8862..9590)
                     /locus_tag="PK399_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPW12825.1"
                     /translation="MDTQRNGIGLVGFDADDTLWRSQDYFDDAQAQFERIVAGYVDLD
                     DVAGRLYAVEKRNLALFGYGVKGMVLSMVEAAVEITGERISAGDLHRIVGLGKALLGH
                     PVELLDGVREAVQAIAATHPVVLITKGDLFHQEAKVRDSGMADLFRRIEIVSEKDPAT
                     YARLFEEFGIAPGRFLMVGNSLRSDIAPVIELGGWGVHVPYHTTWAHEADARLLHGGE
                     RMRTIAAIADLPQAVQELAAIAAG"
     gene            complement(9729..10097)
                     /locus_tag="PK399_06625"
     CDS             complement(9729..10097)
                     /locus_tag="PK399_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HPW12826.1"
                     /translation="MQKTNTRNRNTLAILSFALASALAAPAFAQSAPMTTQDPAAAQS
                     APQQATEAAPQAAPAQKTWAELDANGNGSLSASEAAPIESLSKVFAKADKDGNGELTG
                     DEYKAWLAANGGGKPKAGQG"
     gene            complement(10321..11106)
                     /locus_tag="PK399_06630"
     CDS             complement(10321..11106)
                     /locus_tag="PK399_06630"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPW12827.1"
                     /translation="MPRRPALVLLVLCAAIGAADARTVYRCVRDATVSLSTAPEPGSK
                     CEAKTLEDNAAMLPNLWGELGVFSGTLYEWKAADGAVVYTTRRMPGATPYLKFTVETP
                     RGSPAHEGLGQLGKPRLDAHATTFRKTAKARKVDDAFLRAIAHAESGFDDDAVSPKGA
                     QGVMQLMPDVQRDYGVRDPFDPAQSIDAAARHLRALLGRYKGDYALAAAAYNAGIGTV
                     AKYRGVPPYAETIAYVAKVQALHARYRAAMKLPPLDPPLRAAQ"
     gene            complement(11158..11697)
                     /locus_tag="PK399_06635"
     CDS             complement(11158..11697)
                     /locus_tag="PK399_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1453 domain-containing protein"
                     /protein_id="HPW12828.1"
                     /translation="MATPAMTYAVLAPVVLLVLYRRLRRHIGRQPLQPWRIGLRLAGV
                     CIAALALLLIGVAGALGGSMAGVGVLAGLAGGAMLGSYGLRLTAFETNAGGRFYTPNP
                     WLGIGLSALMLGRLAYRFVAVRGASQVALMGDSTALGILHRSPLTLAIAGLLVGYFLR
                     YSVGLWRATRAPVAQATAP"
     gene            complement(11774..12247)
                     /locus_tag="PK399_06640"
     CDS             complement(11774..12247)
                     /locus_tag="PK399_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPW12829.1"
                     /translation="MRANDIPLDDRDRAILRELQADGRLTNLELAQRINLSPSACLRR
                     VKLLEESGLIARYVMLLDEKLAGLPGTAFVLVTLDQQGRAALDAFESAINRHPEVTEC
                     CLLAGTADYMVRVAYADSVDFERIHTEILTQLPGVVRVQSTLALRTVKKTTALPV"
     gene            12401..13519
                     /gene="ald"
                     /locus_tag="PK399_06645"
     CDS             12401..13519
                     /gene="ald"
                     /locus_tag="PK399_06645"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPW12830.1"
                     /translation="MRVGVPKEIKNHEYRVGLVPSSVLELVHHGHEVLVQKGAGLGAG
                     LTDEDYIAAGAKIIETADEIFASADMILKVKEPQADERKKLRPGQILFTYLHLAPDAP
                     QTKDLIDSGAVCIAYETVTAANGSLPLLTPMSEVAGRLAPQVGAHALEKAQGGRGVLL
                     GGVPGVPAAEVVILGGGVSGTHAATIAVGMGAQVTVVDRSADALKRLASQFGTSISTV
                     FSTRSAIEELVRRADLLIGTVLVPGAAAPKLVTRDMLKTMKPGSVIVDVAIDQGGCVE
                     TSHATTHADPTYVVDGVVHYCVANMPGAVARTSTFALNSVTLPFTLALADKGWRKALE
                     QDPHLREGLNVCEGKLTCEPVAEAHGLAYTSAESVLGL"
     gene            13628..14152
                     /locus_tag="PK399_06650"
     CDS             13628..14152
                     /locus_tag="PK399_06650"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPW12831.1"
                     /translation="MTHTHWTRLIRDIPDFPKPGIVFKDIMPVLADAAAFDDAIEALA
                     APWLGVRIDAVLAMEARGFLFGAPLARALGAGIVPLRKPGKLPGRVFTQRYALEYGED
                     ALQVQVDAVPAGARVLLADDVLATGGTLLAAHGLAKQLRAEVAGASMLIELGFLNGRA
                     RWDGDAPLHAALVV"
     gene            complement(14163..>15258)
                     /locus_tag="PK399_06655"
     CDS             complement(14163..>15258)
                     /locus_tag="PK399_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HPW12832.1"
                     /translation="GLEHPRWLHVLPNGDVLVAESNAPAGKSGVDGIKGKVMGVVMKR
                     AGAGVPSAERISVLRDADGDGIAEVRQPFLTGLHSPFGMALVGDTLYIANADALVSVP
                     YRTGQLRIDAVPLKVVDLPGKGDELNHHWTKSLLASRDGARLYVGVGSNSNVAENGMA
                     VETNRAAVLEVDPATRRVRVFASGLRNPVGLALEPQGGALWAVVNERDELGNDLVPDY
                     LAEVREGGFYGWPYSYYGQHLDPRVEPQAPALVARAIKPDYALGSHVAPLGLAFTPGG
                     AWSGGQGGAVIGLHGSWNRKPQVGYKVVFVPFAGGRPVGKMQDLLTGFLDAGGNARGR
                     PVGIAVQADGGILVADDVGNTVWRLAAARD"
BASE COUNT         2539 a         5278 c         5115 g         2326 t
ORIGIN      
        1 gcttcttgcg caggcggccg atgaagacct ccagcacgtt ggaatcgcgg tcgaagtcct
       61 gctggtagat gtgctcggtg aggtcggcct tcgagaccag ttcgccggca tgcatcatca
      121 ggtactcgag caccttgtat tcgtagctgg tcaggtcgac gttgccgccg ttgacgctca
      181 cggtctgcgc cgccaggtcc agcatcaccg gcccgcattc cagggtcggc ttgctccagc
      241 cagcagcgcg gcgcacgagc gcattgatgc gggccagcag ttcctcgaca tggaagggct
      301 tgaccaggta gtcgtccgcg ccctgcttga ggccatcgac cttgtcctgc cagctggaac
      361 gcgcggtgag gatgagaacg gggaatttct tgccttcgtc gcgcagggcc ttgatcagtt
      421 ccatgcccga catcttcggc aggccgaggt cgatgatgcc gacatcgaac ggcacttcgc
      481 gccccatgta caggccttct tcgccgtctt gcgcggcatc caccgcatag ccttcgcgct
      541 tcaggcgcgc agccagggtc tcgcgcagcg gggcttcgtc ttcgacgagc aggattcgca
      601 tgggaactcc tcggaaatgg ggatcgggtt gcccgatact tcaggctgaa cggattcagc
      661 ttgcgcgatc agtcgtcatc atcgcgtgca ggcggccggc gcggcgcctg cccacggtca
      721 ttgttgccac caccatcgtt gttgccgccg ctgcgcggag gcgcaccacc gtcgcggcgc
      781 tggggatcct gcatgaacac gcgcacgcga ccctggtcgt ccaccacctt gacccggtgc
      841 atgtcgcggc cgtcgtactg cacgcgctcc gccgacagca cttcgccgcg ggtgtcgcgc
      901 tctacccggc gaaccgcatc cgacaggctg ccacgacggt tgtcgtcgtt gccgcgggca
      961 tcgccacgat cccggggcgg cggtggcggt gcctgctgcg cccatgcgga agccgcaggc
     1021 agccctgcca ggaagaccac gcacaacagc ttcgacaggg gcttgggaac tcgatacatg
     1081 gcggggggcg acatcgggat agcgggaacg gctgccattc tggaacctgg cggtgaatcc
     1141 gccctgaagt ttcctgaaca ggataccccg ccattcaggc acatgtgcgt ggcgtgatgc
     1201 cgtgaccgcg cgctcaatac acctcgccga tgcagcaacg cagggatccg cccgcagcct
     1261 cgatcgcatc cagaaccacg gtttcgaccc ggaaaccggc atcggccaac gcttgccggt
     1321 gggtggccga cagcgccgcc ccggcattcg cgctcatcca gaccgtgtct gccgacagcg
     1381 cgatgcagtt gccgacgaag gccgcgtgtt ccgcgtcatc gcagaccacc gcgtgcggtg
     1441 cgtacaggcc cgcgatcgcg gccggcaccg ccggatcggc gaatccgcgc gggcagacca
     1501 ccgccgcgcg accggccagg accgccagca ccacgttggt gtggtactcg ccgggtgcaa
     1561 gatcgaacag cagggtcgcg cgcaacccga acgcatcgtg catcagccgg gcaccagcct
     1621 ggtcacagcg ctcggacagg ccgcagaagc ccaagccgct ggcgcggtcg atcaccaggg
     1681 ccccggtcag ttcgcaggga tgcggctgca cggagaggtc gacttcatcg cgccccagca
     1741 tctcggcgaa gaacccgcgg atatcgctgc ggatcgcttc acgctggcgc accggatggc
     1801 gcatgcgtcc gatgatgcag cgccccgcgg cggtgccgaa cacgttgttg gggaacagcg
     1861 cgtccggcgt ggccggatcg ccggcgaagc agacggtcgg cagcaccgtg gatatcgcgc
     1921 gatgaaggtt gcgatgctgc atcgacgcgc gttgtgcgtc gaacgccgcg gcatccgcca
     1981 tgtaggcgtt gtcctgcgca gactgctcgg cgagggcgaa accgtcaggc gcgaccagga
     2041 aggcggcgcg cgcggtggcg ggaccgaaat ccggcgcagc atcacgggcg aaggccagga
     2101 atgcgtcgat gtcgcgcgtg atcatgcaac ccgttccctg cgttcggtga gcgccagcgc
     2161 ctgttcgacc agcttccagt cggtacccgg cttgtgcgcg ccttcgctca acacctggcg
     2221 gaacgcgcga ccacccggtt gtccggcgaa caggccgagg acgtggcgga cgatgtgctt
     2281 gagcaacacc ccgcgcgcca gctgcgcctg gacatagggt cggtaggcgc gcagcagctc
     2341 gctgcgcgac accaccgcgc cgccgaacca ggcgacatcg agttcgtgca ggagataggg
     2401 cgtgtggtag gcggcgcggc ccagcatcgc cccatcgaca tgacccaggt gcgcggtcgc
     2461 ttcggccgca tcggcgatgc cgccgttcac taccacctgc aacgtgggtc gctcctgttt
     2521 caaccgatac gcccagtcgt agcgcagcgg cgggacttcg cggttttcct tcggcgacaa
     2581 cccctgcagc caggcattgc gcgcatgcac cacgaaggtc acgcagcctg cggcggcaat
     2641 cgtgtcgatg aaggcgagga agcgtgccca ctcgtggtcg tcgtccacgc cgagccggca
     2701 cttcaccgtc acggggacgc cgggcacggc ggcgatcatc gccgccacgc tgtccgcgac
     2761 cagcgccggt tcgcgcatca ggcaggcacc gaagcggccc gcctgcacgc ggtcggacgg
     2821 gcagccgcaa ttgaggttga tttcgtcgaa accggcatcc acgccgatcc gcgcggcctg
     2881 ggcgagcaag gccggctcgc taccgccgag ctgcaaggcc accgggtgtt cgcacggatc
     2941 catcgccagc aagcgcgcgc gatcgccgtg gatcaccgca ttcgcgtgca ccatctcgct
     3001 gtacagccgc gcgtgcggcg ccagcacgcg atggaaggcg cggcaatgcg catccgtcca
     3061 gtccatcatc ggggcgacgg acaggcgcag ctggtggagg gggatggcgg gcgcggcggt
     3121 catgcgggcg ccattgtacg gacgaccttc gtccaactgc gtgcgacggg gcgatgcgcg
     3181 acaatgacgc cctgacgcga acacagccaa ggcccatgaa cgtcgatttc cgcaagcccc
     3241 tgcccggcac cccgctcgac tggtacgacg cccgcgccgc cgtcgacgcg ctgcgcccgg
     3301 gcgcctacgc cgcgctctcc tacaccgcgc gcgtgcatgc cgagaacatc gtccgcaagg
     3361 ccgatccggc gatgcgcgac gcgtaccttc tgcaactgat cgaacgccgc cgcgacctcg
     3421 acttcccgtg gttcccggcg cgggtggtct gccacgacat cctcggccag accgcactgg
     3481 tcgacctggc cggtttgcgc gatgcgatcg ccgaacaggg cggcgatccg gcgcaggtca
     3541 atccggtggt accggtgcag ctgatcgtcg atcactcgct ggcggtggaa tgcggtggct
     3601 acgacccgca ggcattcgag aagaaccggg cgatcgagga tcgccgcaac gccgaccgct
     3661 tccacttcat cgactggacc aagcaggcgt tcgagaacgt ggacgtcatc ccgccgggca
     3721 acgggatcat gcaccagatc aacctggaga agatgtcgcc ggtgatctac acgcaggacg
     3781 gcgtcgcgtt tcccgacacc tgcgtgggca ccgactcgca cacgccgcac gtggatgcgc
     3841 tgggggtgat cgccatcggc gtcggcggac tggaggcgga gaacgtgatg ctgggccgcg
     3901 cgtcgtggat gcgcacgccg gagatcgtgg cggtggaact caccggcaag ccacaggccg
     3961 ggatcaccgc caccgacgtg gtgctggccc tcaccgaatt cctgcgcaag cagaaagtgg
     4021 tcggcgccta cctggagttc cgtggcgaag gcgctgccgc attgaccatc ggcgatcgcg
     4081 cgacgatcag caacatggcg ccggagtacg gcgcgaccgc ggcgatgttc ttcatcgacg
     4141 gcaacaccct ggactacctg cgcctgaccg gccgcgagga acagcaggtg caactggtcg
     4201 agctctacgc caaggctgca ggcctctggg ccgatgacct ggcggctgcg cagtacgaac
     4261 gcacgttgca cttcgacctg tccagcgtgg tgcgcaacat ggccgggccg tccaatccgc
     4321 acaagcgcct gcccaccagc gacctggccg aacgcggcat cgccggcaac ctggagggcg
     4381 cgctgaagca ggaagcgcag ggcctgatgc cggatggcgc ggtgatcatc gccgccatca
     4441 ccagctgcac gaacaccagc aacccgcgca acgtgatcgc cgccgcgctg ctggcgcgca
     4501 acgcgaacgc gcgcggcctg acgcgcaagc cgtgggtgaa gacctcgctg gcgccggggt
     4561 cgaaggccgt gcagctgtac ctggaggacg ccggcctgct cagcgaactg gaacaactcg
     4621 gcttcggcat cgtcgccttc gcctgcacca cctgcaacgg catgagcggc gcgctggatc
     4681 cgtcgatcca gcaggagatc atcgagcgcg acctgtatgc cacggcggtg ctgagcggca
     4741 accgcaattt cgacgggcgc atccatccgt acgcgaagca ggcgttcctg gcctcgccgc
     4801 cgctggtggt ggcctatgcc atcgccggta ccgtgcgctt cgacatcgaa caggacgtac
     4861 tgggcctggg ccacgacggc aaaccggtga cgctcaagga catctggccg agcgacgcgg
     4921 agatcgacgc ggtagtgaag gccagcgtca agcccgagca attccgcaag gtctacgggc
     4981 cgatgttcaa cgtgcgcgtg gaacacggtt caccggtcag cccgttgtac gactggcggc
     5041 cgaagagcac ctacatccgc cgcccgccgt actgggaagg ggcgctggcc ggcgcacgca
     5101 cgctacgcgg catgcgcccg ctggcggtgt tgccggacaa catcaccacc gaccatctct
     5161 cgccgagcaa cgcgatcctg ctcgacagcg cggccggcga atacctcgcg cgcatggacg
     5221 tgccggaaga ggatttcaac tcctacgcga cccatcgcgg cgatcacctg accgcacaac
     5281 gcgccacctt cgcaaacccg aaactcatca atgaaatggc cgtggtcgat ggcatggtgc
     5341 agcagggctc gctggcacgc gtcgaacccg acggcaaggt gatgcgcatg tgggaagcga
     5401 tcgaaaccta catggaccgc aagcaaccgc tgatcatcat cgccggtgcc gactacgggc
     5461 agggcagctc gcgcgactgg gcggcgaagg gcgtgcgcct ggccggcgtg gaagcgatcg
     5521 tggccgaggg cttcgagcgc atccaccgca ccaacctggt cggcatgggc gtgctgccgc
     5581 tggaattccg gaacggcgac acccgccaca cctacgccat cgacggcacc gagaccttcg
     5641 acgtggtcgg cgaccgcacg ccgggcgcga cgttgacgct ggtgatccat cgaatgaacg
     5701 gcgagcgcat cgaagtcccc gtcacctgcc ggctggattc cgacgaggat gtcgaggtgt
     5761 acgaagcggg cggggtcctg cagcgcttcg cccaggattt cctcgcggcg aacaaggcgg
     5821 cgtgagatga cgacctccca ctcaccacgc cacgctccgc aactgcgtgt tcccgcgacc
     5881 tacatgcgcg gcggcacgtc gaaaggcgtg ttcttcacgc tggacgacct gcccgacttc
     5941 gcacgcgcgc cgggcaagcc gcgcgatgcg ctgctgctgc gggtgatcgg atcgccggat
     6001 ccttacggca agcagaccga cggcatgggc ggggcgacgt cgagcaccag caaggtggtg
     6061 atcatcgcga agtcggcggt cccgggccac gatgtcgact acctctacgg ccaggtgccg
     6121 atcgagggcg acttcatcga ctggagcggc aactgcggga acctcagttc ggcggtgggc
     6181 ccgttcgcga tcgccaacgg gctggtcgat gccgaccgcg tgccgcgcac cggcatcgcg
     6241 accgtgcgga tctggcaggc caacatcggc aagacgatcg tggcgcacgt gccgatgacc
     6301 gagggcgagg tgcaggaaac cggcgacttc gaactggacg gcgtgacctt cccggccgcc
     6361 gagatcgcgc tggaattcct cgatcccgcc gacgaaggcg aagacggcgg cgcgatgttc
     6421 ccgaccggca acctgaccga tgaactcgac gtgcccggcg tcggcgcttt ccgggccacg
     6481 ttgatcaatg ccggggtgcc gatggtgttc gtcgatgccg cggcactggg cttcgacggc
     6541 accgaactgc aacccgcgat caatgcagac aaggcgatgc tggccaggct cgaagcgatc
     6601 cgcgccgtcg gtgcggtgcg catgggcctc atccacgacg tgtcggaggc cgcggcgcgg
     6661 ccgttgacgc cgaagatcgc cttcgtggcg ccgccgaagg actacgtgtc gagcagcggc
     6721 aagggcatcg atgcgacggg catcgacctc aacgcgcgcg cgatctcgat gggcaagctg
     6781 caccacgcga tgatggggac ggcgtcggtc gcgatcgcca ccgcggcggc ggtgcccggc
     6841 accgtggtca acaccgcagc aggcggtggc agccgtggcg cggtgcgctt cggccatccc
     6901 tccggcacga tgcgggtggg cgccgacgtg gcgcaggtcg acggccagtg ggtggcgacc
     6961 aaggcggtga tgtcgcggtc cgcgcgcgtg ctgatggaag gcgtggtgcg ggtgccgcgc
     7021 gggcaggtcg cggcggatta acccgccgcc tcgtcgaacg cgaagccggc cttgcgcagt
     7081 tcgtggcggg cctggtcgaa gccggtaccc ggcaccattt cgccatcgcg ttcggccagt
     7141 tcgccccagc gctccagcag ggcttccgcg gtcggcgtcg tgcccaggtg gatgccttcc
     7201 gtcagggtga tgtgcgccaa ctcgtagccg ccggcgccgg cgcacaggat cgcgcgggtc
     7261 ggtgcgccgt cggccaccag cgcgagcacc gccgggctga ccagcgcggg gtccagcatg
     7321 tccaactgat cctgcggcag gatgccttcc atcatccgcg tgtacgcggt cggcgccagg
     7381 caattgacct tgatgccgtg cttcgcgcct tccagcgcca gcgtctgcat cagcccgacc
     7441 aaggccatct tcgccgcagc gtagttggcc tgaccgaagt tgccgtacag gccggacgag
     7501 gacgtcgtca tcacgatgcg gccgtacgcc tgcgcgcgca tcgcgtccca cacggccttg
     7561 gtgcagttcg ccgcgcccat caggtgcacg tcgaccactg cgcggaaatc gtccagcggc
     7621 atcttcgcga acgacttgtc gcggaggatg ccggcgttgt tgaccaggat gtcgacgcgg
     7681 ccccactcgg ccagcacggc gtcgaccatc gcctgcaccg cgtcggcgtc tgtgacggaa
     7741 cactgcagcg cgcgcgcctg gccgccggcc gcgacgatct ccgcggccac cgcctgcgcg
     7801 gcatcgccca ggtcgttcac caccaccctg gcgccacgac gggcgagcag caacgcatgc
     7861 tgccggccaa gcccgccacc cgcgccggtc acgatggcca cgcggcccgc caggtcgatc
     7921 cggtgttcgc aagtgctcat ggacgctcca ggtgcgcgat cgccgccatc gccgcatcgc
     7981 ggtcgcggtc atcgaaggga atgatgttca aggcagccag gtcgatatcg tccaggcagc
     8041 gtgcgttgac gctgaaaccg tcgggatggc tgcgcggcac gtagaagccc ttgatcccgc
     8101 agtgccggca gaaccggtgt ttggcggcgc cggtgttgaa ggtgtattcg gcgaggtcgt
     8161 ccgcgccgga caggagtcga aaacgcgatg ccggtacgat cacatgcagg aacccggtca
     8221 tcctgcagat cgaacaattg cagtcgagca cgtcggggtt ggcgggtgcg tccacctcga
     8281 agcgcacgcg gccgcaatgg cagccaccgc ggtggacgat gagggaaggc tcttggaggt
     8341 cggtcatccg cccagtctag cgatgcgcgc cgatcaccgg atcaacgatc accttcgaag
     8401 ctggtcggca gcaatacgtc gtcggcaagc gctggcagga gcgcgccatc ggtgccgcgc
     8461 gcctgcaggc tgccgtcgtc ctggcgcagg tacacgcgct cgccaccctg tgccgagcgc
     8521 aaccgcagca ggtcgccgcg cgcggtccag tcgccttcgt cctcgaagcg caggtcgcgc
     8581 ccctcgccac ggtaacgctc caccagttga taccgctgca ggccagcacc cgactgcagg
     8641 cgcaaccagc tgtcgatgcc ggcgcaatcc acgcagggac gctcgccgcg gaattcgagt
     8701 gcgctgtcca ccgcgcccgg atcgatggcg ggcaatctcc tgcccgtcgc aggcgcgcgc
     8761 ggcgcggccg catcgcaacc gacgagcagc agcaggcaca acagcaacgg gggcgatcga
     8821 cgcgacatcc gcggattgtg catcaaggca ccgcgagcgc ttcaaccggc ggcgatcgca
     8881 gcgagttcct gcacggcctg cggcaggtcg gcgatggcgg cgatcgtgcg catgcgctcc
     8941 ccgccatgca gcaggcgcgc gtcggcctcg tgggcccatg tcgtgtggta cggcacatgc
     9001 acgccccatc cacccagctc gatcaccggg gcgatgtcgg agcgcaatga attgccgacc
     9061 atcaggaagc gaccgggcgc gatcccgaac tcctcgaaca ggcgcgcata ggtcgccgga
     9121 tccttttcgc tgacgatctc gatgcggcgg aacagatcgg ccatgccgct gtcgcgcacc
     9181 ttggcttcct ggtggaacag gtcgcccttg gtgatcagca ccacgggatg cgtggcggcg
     9241 atggcctgta ccgcctcgcg cacgccatcc agcagttcaa ccgggtgccc caacaaggcc
     9301 ttgcccaggc cgacgatgcg atgcaggtcg ccggcgctga tccgttcccc ggtgatttcc
     9361 accgccgcct cgaccatcga caggaccatg cccttcacgc catagccgaa cagcgcgagg
     9421 ttgcgcttct ccaccgcgta caggcggccg gcgacatcgt ccaggtcgac gtaaccggcc
     9481 acgatgcgct cgaattgcgc ttgggcatcg tcgaaataat cctggctgcg ccagagggtg
     9541 tcgtcggcat cgaagccgac caggccgatg ccgttgcgtt gcgtgtccat gcgcaaagtg
     9601 tacgtgcagg caaaccaaag tcggcttgcg ccgccacctg cccgaagaaa aaatcgaagg
     9661 gcggccggag ccgcccttcg tgccacaacc tttcttgttg ttgtgcggtg ttgcgtgacg
     9721 gccttgcgtc agccctgccc ggccttgggc ttgccaccgc cattcgccgc aagccaggcc
     9781 ttgtactcgt ccccggtcaa ctcgccgttg ccgtccttgt cggccttggc gaagaccttg
     9841 gacaggcttt cgatcggcgc ggcttcgctg gcgctcaacg aaccattgcc gttggcatcg
     9901 agttcggccc aggtcttctg cgccggtgcg gcctgcggcg ccgcttcggt ggcttgctgc
     9961 ggtgccgact gcgctgcggc aggatcctgc gtggtcatcg gcgcgctttg cgcgaatgcg
    10021 ggcgcggcga gcgccgacgc cagcgcgaac gacaggatcg cgagggtgtt gcggttgcgc
    10081 gtgttggtct tctgcatgaa ttggctcctc gttctgcggg ggatcaaaac ggatcttcag
    10141 gttgcggttg cgggatgcgc cgctgacagg gccaacgcac cccgcaactc acgattcgca
    10201 ccttacgacc tgcgtctgaa cgccttccgc cagccaacat gaacgcaaac atttgcttaa
    10261 ccacgctcgc gggaagacac gctaatcgcg aggcgcattc aacaaacagc ggcttgcccg
    10321 tcactgcgcc gcgcgcaacg ggggatccag tggcggcaac ttcatcgccg cgcgatagcg
    10381 cgcgtgcagc gcctgtacct tcgccacgta ggcgatggtc tcggcatacg gcggcacgcc
    10441 gcggtatttc gccaccgtgc cgatgccggc gttgtacgcg gccgcggcca gcgcatagtc
    10501 gcccttgtag cgacccagca gcgcgcgcag gtggcgtgcc gcggcatcga tggattgcgc
    10561 gggatcgaac gggtcgcgca cgccgtagtc gcgctgcacg tccggcatca gctgcatcac
    10621 gccctgcgcg cccttgggcg acaccgcgtc gtcatcgaag ccgctttccg cgtgggcgat
    10681 cgcgcggagg aacgcgtcat cgaccttgcg cgccttcgcc gtcttgcgga acgtggtggc
    10741 atgcgcatcc agtcgcggct tgcccaactg gcccaggcct tcatgcgcgg gcgaacccct
    10801 cggcgtttcg acggtgaact tgaggtacgg cgtcgccccg ggcatgcgcc gggtggtgta
    10861 gaccacggca ccatccgcgg ccttccattc gtacagggtg ccgctgaaga cgcccagttc
    10921 accccacagg ttgggcagca tcgccgcgtt gtcttcgagc gtcttcgctt cgcacttgct
    10981 gcccggctcc ggcgcggtgg acaggctgac ggtcgcatcg cgcacgcagc ggtagaccgt
    11041 gcgcgcgtcg gctgcaccga tggcggcgca gagcaccagc aggaccaggg cgggacgacg
    11101 aggcatggcg cggattgtag ggaggccggc gtgatgccga gctccccggg caccaactca
    11161 gggcgcggtg gcttgggcca cgggtgcacg cgtcgcgcgc cacaggccga cgctgtagcg
    11221 caggaaataa cccaccagca gtccggcgat cgccagggtc aacggactgc ggtgcaggat
    11281 cccgagtgca gtcgaatcgc ccatcaacgc gacttgcgac gcgccgcgca cggcgacgaa
    11341 gcgatacgcc agccggccga gcatcaaggc ggacaggccg attcccagcc acggattggg
    11401 cgtgtagaag cgcccgcccg cgttggtttc gaacgccgtc agccgcaagc cgtaacttcc
    11461 cagcatggcg ccgccggcaa gtcctgccag cacgccgacg cccgccatcg agccacccag
    11521 cgcacccgcc acgccgatca gcagcaaggc caaggcggcg atgcagacgc ccgccaggcg
    11581 aagcccgatc ctccatggct gcaatggctg ccgcccgatg tgccgcctca gcctgcgata
    11641 caggacgagc agcaccaccg gtgcaagcac ggcataggtc attgcaggcg tggccattcg
    11701 gtttccttcg tcctgggcac gtgcattgtc tgccactgca ccgcgccgtg cagcccggca
    11761 tcgccttgcc gcatcacacc ggcagcgccg tggtcttctt caccgtgcgc aatgccagcg
    11821 tggattgcac gcgcaccacg ccgggcagct gggtgaggat ctcggtgtgg atgcgctcga
    11881 agtccaccga atcggcatag gccacgcgca ccatgtagtc ggcggtaccg gccagcaggc
    11941 agcattcggt gacttcggga tggcggttga tcgcggactc gaaggcatcc agcgcggccc
    12001 gcccctgctg gtccagggtg accagcacga acgcggttcc gggcaggccg gccagcttct
    12061 cgtccagcag catcacgtag cgcgcgatga gcccagactc ctccagcaac ttgacccggc
    12121 gcaggcaggc cgagggcgac aggttgatgc gctgcgcgag ctcgaggttg gtcagccggc
    12181 catcggcctg cagctcgcgg aggatcgcgc ggtcgcggtc atcgaggggg atatcgttgg
    12241 cacgcatatg attcgacgat caggccactg attcgccaac aatgcgacat ccacgcattc
    12301 atcgcaacga attcggcgat taaatgcgcg atcccacgcg catactgccg tccttcaggc
    12361 gccgggcccg ccggtgccgc ccacgggaga gggactgcac atgcgcgtcg gcgtaccgaa
    12421 ggaaatcaag aaccacgaat accgcgtcgg cctggtcccg tcgtcggtgc tggaactggt
    12481 ccaccacggc cacgaagtgc tcgtgcagaa gggcgcaggc ctcggcgcag gcctcaccga
    12541 cgaggactac atcgccgccg gtgcaaagat catcgaaacc gccgacgaga tcttcgccag
    12601 cgccgacatg atcttgaagg tgaaggaacc gcaggccgat gagcgcaaga agctgcgccc
    12661 gggccagatc ctgttcacct acctgcacct ggcgcccgat gcgccgcaga ccaaggacct
    12721 gatcgactcc ggcgcggtct gcatcgccta tgaaaccgtc accgcggcga acggttcgct
    12781 gccgttgctg accccgatgt cggaagtcgc cggccggctg gccccgcagg tcggcgcgca
    12841 tgcgctggag aaggcgcagg gcggccgcgg cgtgctgctg ggcggcgtgc ccggcgtgcc
    12901 ggcggcggaa gtcgtgatcc tcggcggcgg cgtctccggc acccacgcgg ccacgatcgc
    12961 ggtgggcatg ggcgcgcagg tcaccgtggt cgaccgttcg gcggatgcgc tgaagcggct
    13021 ggccagccag ttcggcacct cgatctccac cgtgttctcg acccgttcgg cgatcgagga
    13081 actggtacgt cgcgccgacc tcttgattgg caccgtgctg gtgccgggtg cggccgcgcc
    13141 gaagctggtc acccgcgaca tgctgaagac gatgaagccg ggctccgtga tcgtcgacgt
    13201 cgccatcgac cagggcggct gcgtggaaac ctcgcacgcg acgacgcatg ccgacccgac
    13261 ctacgtggtc gacggcgtgg tgcattactg cgtggccaac atgccgggcg cagtcgcgcg
    13321 cacctcgacc ttcgcgctca acagcgtgac cctgccgttc accctggccc tggccgacaa
    13381 gggctggcgc aaggcgctgg agcaggatcc gcacttgcgc gaaggcctca acgtctgcga
    13441 aggcaagctc acctgcgagc cagtcgccga ggcgcacggc ctggcctaca ccagcgcgga
    13501 gagcgtgctg ggcctctgat cctgcttgtg gcagggatgc cccgaagggg acggcgtgcc
    13561 gtccccttcg tgtttgcggc atgcgcgacg cacggcatga tgacggaccg tttccgatgc
    13621 cgccgccatg acccataccc actggacccg cctgatccgc gacatccccg acttcccgaa
    13681 gccggggatc gtgttcaagg acatcatgcc ggtgctcgcc gatgccgcgg cgttcgacga
    13741 tgcgatcgaa gcgctcgcgg cgccgtggct cggcgtgcgc atcgacgcgg tgctggcgat
    13801 ggaagcgcgc ggcttcctgt tcggcgcgcc gctggcacgc gcgctgggcg ccggcatcgt
    13861 gccgctgcgc aagcccggca aactgccggg ccgggtgttc acccaacgct acgcgctgga
    13921 gtatggcgag gacgcgctgc aggtgcaggt ggacgccgtg ccggccggcg cgcgcgtgct
    13981 gctggccgac gacgtgctcg cgaccggtgg caccctgctc gccgcgcatg ggctggcgaa
    14041 gcagttgcgg gccgaggtgg ccggcgcgtc gatgctgatc gagctgggtt tcctcaacgg
    14101 acgcgcgcgc tgggatggcg atgcgccgct gcacgcggca ttggtggtct gacgccgcgc
    14161 tgtcagtcgc gagcggccgc cagccgccac accgtgttgc cgacatcgtc ggcgaccagg
    14221 atgccgccat ccgcctgcac cgcgatcccg accggccgcc cgcgcgcatt gccgcctgcg
    14281 tccaggaaac cggtcagcag gtcctgcatc ttcccgacgg gtcggccgcc cgcgaacggc
    14341 acgaacacga ccttgtagcc cacctgcggc tttcggttcc aggagccgtg caggccgatc
    14401 accgcaccgc cctgcccgcc gctccacgca ccaccgggcg taaacgccag gcccaacggc
    14461 gcgacatgcg aacccagcgc atagtccggc ttgatcgccc tggccaccag cgccggtgcc
    14521 tgcggctcga cccgcggatc caggtgctgc ccgtagtagc tgtagggcca gccgtagaag
    14581 ccaccctcgc gcacctcggc caggtaatcg gggaccaggt cgttgccgag ttcgtcgcgc
    14641 tcgttcacca ccgcccacag ggcgccgccc tgcggttcca aggccaggcc caccggattg
    14701 cgcaggcccg acgcgaacac gcgcacgcga cgcgtcgccg gatcgacttc caggaccgcg
    14761 gcgcgattcg tctccacggc catgccattc tcggcgacat tgctgttgga ccccacgccg
    14821 acatacagcc gcgcaccatc gcggcttgcg agcaggctct tggtccagtg gtggttcaac
    14881 tcgtcgccct tgccgggcag gtccacgacc ttcaacggca ccgcgtcgat gcgcagttga
    14941 ccggtgcggt aaggcacgga caccagcgca tcggcgttgg cgatgtacag cgtgtcgccc
    15001 accagggcca tgccgaaggg cgaatgcagg ccggtgagga acggttggcg gacttcggcg
    15061 ataccgtcgc catcggcatc acgcaacacg ctgatgcgct cggcgctcgg aacgccggca
    15121 ccggcccgct tcatcaccac gcccatgacc ttgcccttga tgccgtcgac accgctcttg
    15181 ccagccggcg cattgctctc cgccaccagc acgtcgccat tcggcagcac atgcagccag
    15241 cgcggatgct ccaggccg
//
LOCUS       DAOOCX010000053        15229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3525_length_15229_cov_9.098194, whole genome shotgun sequence.
ACCESSION   DAOOCX010000053 DAOOCX010000000
VERSION     DAOOCX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3525_length_15229_cov_9.098194"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PK399_06660"
     CDS             complement(<1..621)
                     /locus_tag="PK399_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW12833.1"
                     /translation="MSSFNPRHKIATRTRLSEVRYEIRGELARRARELEGQGRSLIKL
                     NIGNPGAFGFRAPDHLQRAIADHIANTDPYTHQQGLPEAREAIAAFHKARGTPNASPE
                     RVFVGNGVSELIDISLRALLNPGEEVLLPSPDYPLWSAATILNDGRPVYYRCDEANGF
                     LPDPDQIESLVSSRTRAIVLINPNNPTGANYPRALLERIVAIAQKHR"
     gene            836..1936
                     /gene="rsgA"
                     /locus_tag="PK399_06665"
     CDS             836..1936
                     /gene="rsgA"
                     /locus_tag="PK399_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011052312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPW12834.1"
                     /translation="MTPLQAIGWRWTPATLDPAWAALMAANPDARPARITEQHRSGYA
                     VSEAIDVAHRAESLPEWQRAGSYRKGEINPESRPAVGDWVLVEGEAPNALRIVALLPR
                     FSAIKRGAAGEHYKQQVIAANIDTVFVVCGLDADFNPRRIERYLLLVGGSGVTPVIVL
                     TKSDKDGADVEAALHELRAFGTPVLALNAKDRDSAAALAPWLGDGQSIVLVGSSGAGK
                     STLTNTLLGSERMKTGEVRESDARGRHTTTHRALIALPSGACMIDTPGMRELKPTGEE
                     VVAESFADVEALAAQCKFNDCKHLKEPGCAVRAAIERGEVDAQRVANFLKLSAEVAGA
                     ANQLATRLAQKADAKVQNKALYKRVDEKYGRH"
     gene            2084..>2385
                     /locus_tag="PK399_06670"
     CDS             2084..>2385
                     /locus_tag="PK399_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HPW12835.1"
                     /translation="MNAEIAHHAALDARMAKAARGIRLLSLVSWPASEQQTFLADLAR
                     GNARLPAHQYPTHDFSDARREFDAIAADADPSHPLGIYLIESAHSWSLAAELLESLG"
     assembly_gap    2386..2485
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2486..3139
                     /locus_tag="PK399_06675"
     CDS             <2486..3139
                     /locus_tag="PK399_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1704 domain-containing protein"
                     /protein_id="HPW12836.1"
                     /translation="AAFSDYDRHQLLQHEAFVHSLTALNGQQQAVLPSLSLSSPRTTA
                     TQEGLAVFAEQITGSIDIERMKRVSLRIEAVAMALEGADFLQVFRYFQDAGQDAVESF
                     TSAQRVYRGVPTSGGSAFTKDTVYLRGLIGVHTFFRWALKHDKLRLCRMLFAGKMTLA
                     DVQRFEPLFDSGDLVAPRWLPEWVARANGLAGMLAFSLFANRIRLEQIMDAETVVDL"
     gene            3145..4476
                     /locus_tag="PK399_06680"
     CDS             3145..4476
                     /locus_tag="PK399_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPW12837.1"
                     /translation="MQPTLAVPPPVVLPWYRHLYVQVLVAIALGALLGHFAPGTGEAM
                     KPLGDAFIKLVKMIIGPVIFLTIVTGIAGMTHLRTVGRVFGKAMAYFLFFSTLALVVG
                     LVVANVVQPGAGMNVNVADLDRSVVDGYVSKSHELTLVGFVLDMIPDTLVSAFTGGNI
                     LQVLLVSVLFGIALALSGDRGRPMADFLESLTIPVFRLVRILMQAAPIGAFGAIAFTI
                     GKYGLGVLVQLGWLVGSFYLTSLLFVLVILGTVCRLCGFSVLKLIRYLKAELLLVLGT
                     SSSESALPALMEKMEHAGCGKQVVGLVVPTGYSFNLDGTNIYMTLAALFIAQATNTEL
                     TLGHQVTLLLVAMLSSKGAAGVTGAGFITLAATLSVVPEVPVAGMALILGVDRFMSEC
                     RSLTNFIGNAVATVVVARWENALDRTRLAAVLDGQAVAPIAPLHPVRAMGH"
     gene            complement(4483..5802)
                     /locus_tag="PK399_06685"
     CDS             complement(4483..5802)
                     /locus_tag="PK399_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12838.1"
                     /translation="MATSRPPKPMVMPVLVFALACLLQLPLVLNPGYYSHDELQWAVH
                     AVDGVRVPWLDVSAFQYRPLTFNLWMRLSRTLFDTPMLFHAVLVAAGALNATLLFAVG
                     RGFGMASRPAVIGALAFVLSPYAVYTHGWVGCIADLLWVGCALLLGLWVQHVRRAFPA
                     ALAAALLTAIALLAKEAAFAIPPLLALAWWFDGRKPKWCWAMLASGAVAALYLGLRLD
                     VLLHAPREGAQYTLSLAHVPQRWLEYQLFLPIVPLHEAITTLQRPLPALVAGLSWLGL
                     LAALWQAGRRFAALFLLGGIAALLPVLPLASAWNHYAYGFAAIAAMTIAATWPLASRR
                     GRIAIGIFALLTVLHGGAVMWRMQQVGRIQSVFSPALADAVRTHAQPFPVVVRIGPGI
                     KPWIVQRLTHDIPRHDGIAIGDRVRIAADGEAADYLVEPDGSLQATR"
     gene            complement(5802..7034)
                     /locus_tag="PK399_06690"
     CDS             complement(5802..7034)
                     /locus_tag="PK399_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW12839.1"
                     /translation="MTQHAHATADADVAVIGAGVVGVATAYALARRGLRICVIDRAPG
                     PALQASFANGAQLSYAYTDAMAGPALWKQLPGMLAGRDRAFRTRVSTDPGFWRWVLAL
                     LRNATQARLRANTLATLELALESRAAMAELLMRYPLDFDHRVAGKLHVYYNATSLPAA
                     RAMIERKRPFGVRQQLLDADAAIAIEPALAGARGIAGVVHSPEEEVGDPWRFSSGLLD
                     VLRAHYGVEARFGVDLAHLQRERALWRLHARNGDVVTVPNVIVCAGIASRALLRPPGV
                     RVPLMAVKGYSFTAPCGANAPSASITDTARKLVFCRLGDKIRVAGLADLNHWDAAPDR
                     ARVADLVAMARESLPQAADYDAIESDWAGLRPMTPWSSPIIRRAGDGLVLNIGHGMLG
                     WTLAMGSGERAANLLQDA"
     gene            complement(7042..8253)
                     /gene="gltS"
                     /locus_tag="PK399_06695"
     CDS             complement(7042..8253)
                     /gene="gltS"
                     /locus_tag="PK399_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HPW12840.1"
                     /translation="MIQLDIVQTVAFAGLALFAGYALCRAIPVLRRYNLPEPVVGGLL
                     VALAAWWAHARETPLFELDTTLQSPLMVAFFATVGINASFRLLKASGWPVVLFLLLAT
                     FFAVVQNLVGITVAWLFDLHPMFGVLAGSATLTGGPATGLAFAPAFEAAGLRGAESIA
                     VTAAMAGIVCGGVIGGPAITVLMRRYGITGSPSPGAAPTPVADDVEGRVAHTETDAAR
                     EFNALKAIVVLLLVVWAGGWVMKGFAAINMTMPATIGGMLVGALVRNLDDATDWLRLP
                     VHTLDLLGNICLALFLAMALMNLKLWELAGMGLPLLVNLAVQVALVVLFTVAVFKLMG
                     RDYDAAVMGGGFIGFMLGTTANAMAVMRALAQRYGAAPRAFLVAPLVGAFFIDFNNAL
                     IITGFLNFWPY"
     gene            8371..10692
                     /locus_tag="PK399_06700"
     CDS             8371..10692
                     /locus_tag="PK399_06700"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPW12841.1"
                     /translation="MSDITGNDDLQKDDLKQAALDYHSLEPKGKIKVTATKPMVTQRD
                     LALAYSPGVAFACEAIAADPRMASEYTARGNLVAVITNGTAVLGLGDIGPLAGKPVME
                     GKGVLFQKFAGIDVFDIELNERDPDKLVDIIAAMEPTFGGINLEDIKAPECFIVERKL
                     RERMNIPVFHDDQHGTAIIVGAAILNALEVAGKKIEDVRLATAGAGAAGIACLDMLVA
                     LGLKAENILAFDREGVLYTGRGQMDPDKARYARDTAKRTLAEIVDGADIFLGLSAGGV
                     LAPAMVATMAPTPIILALANPYPEILPEHAKAVRPDCIIATGRSDYPNQVNNALCFPY
                     IFRGALDVGAQEINEAMKLACVRAIAALARKEASDLGSAYGGEVPKFGAEYLIPRPFD
                     PRLLTMLAPCVAQAAMDSGIAERPIADIAAYKEKLGQFIHRTSLMMKPVYERARSERK
                     RVAYAEGEEYVVLQAVQTVIDEGLAFPILIGRPEVIENRIAKLGLRMRAGVDFELTNI
                     NDDPRFDDYWRQYHALTERRGVTPDASKNLIRSRPTIIAALMVERGEADGLITGIVGR
                     YHKKLGYLRSIFDFDTGVQGTAAMTGVINDKGAWFFVDTHVQMDPSAEQIAEATMQAA
                     YRLKLFGIEPKVALLSHSNFGSHQDASATKMRRAYEIVRARAPKLEIDGEMMADTAWD
                     EALRQRIMPNTTLSGRANLLVMPNLDAANIGYNLIRVATGGVAIGPILMGLDQPAHIL
                     TPASSSRRVVNMTAIVAVEAQIRAAMTTAASGK"
     gene            complement(10726..11082)
                     /locus_tag="PK399_06705"
     CDS             complement(10726..11082)
                     /locus_tag="PK399_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW12842.1"
                     /translation="MPALRILLVEDCTDDADLLRFQLEDAGLSFDMRCASSERMLRET
                     LETFQPTVAVSDLNLPGYSGLAALSLLHERLPTLPLVLLTGADDAPAPPVPATVLGKS
                     ELHRLPALLAGIQGGS"
     gene            complement(11087..11527)
                     /locus_tag="PK399_06710"
     CDS             complement(11087..11527)
                     /locus_tag="PK399_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW12843.1"
                     /translation="MTPTIRSILLAEDSPADAEMAIDALREAHLANPIVHVMDGLEAM
                     DYLHRRGKFSDRPEGLPAVLLLDIKMPRMDGLEVLRQLRADEQLKRLPVVVLTSSREE
                     SDLARSWDLGVNAYVVKPVDSEQFFTAVKTLGRFWAVLNETPSE"
     gene            complement(11524..13329)
                     /locus_tag="PK399_06715"
     CDS             complement(11524..13329)
                     /locus_tag="PK399_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW12844.1"
                     /translation="MPQTPAPIRQSVGLIALIGALVMLIVVGPFWVVREANRKSMDAA
                     AIVNHTHEIESTVQGLMYDLRNRESATVAYAYGHDSPAIRERLAESQKEIPVNMARLT
                     QLTRDNPEQQVRIGKLGSIIEQRGMIMETVLAKPAGQADPRDIEWLLDRNPLRHIGAE
                     ILDHEQALLKDRTSEASRLSERSALLTWLAMLLQLLLLGAVAVLLRLQWRNRRAAEAA
                     AARSSARALSVLQTVREPMAVVDRDLRVVLHNPAFREVFAATDAEPGTPIGAFGGGTW
                     EAPETRQRLADVLARDRELWDHEITHRGEDGRERSLLVNARRMALPDRSDDVALVTAN
                     DVTAQKAAELQVRELNRQLIGKVDQVSEVNRELEAFSYSVSHDLRAPLRHIGGFADKL
                     GRHLGDRNDEKTFHYLNTITTSARRMSQLIDDLLVYSRLGRSALRLQPVDMQSVVDET
                     RALLDANNAHDRPGHRIEWKIAPLPIMVGDENMLRQVWLNLLSNAVKYSGGSEPAVIE
                     VGYSRDDHGDYRFEVADNGVGFDMAYAGKLFGVFQRLHSISEFEGTGIGLASVRRVLA
                     RHGGHISADATPGKGARFAFTLPASTDAPQPDTKA"
     gene            13592..>15229
                     /gene="aceE"
                     /locus_tag="PK399_06720"
     CDS             13592..>15229
                     /gene="aceE"
                     /locus_tag="PK399_06720"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HPW12845.1"
                     /translation="MNWLNEMLQNDPDPQESREWIESLKAVIDHDGAGRAHQLLEGMV
                     ELTRRAGAYLPFDPTTSYINTIPTHMEPAMPGDATMEWRIRSMIRWNAMAMVVRANRK
                     PGELGGHIASFASSATLYDVGFNHFWRAPSADHPGDLLYIQGHSSPGIYARAFLEGRI
                     SESQLDNFRMEVDGRGISSYPHPWLMPDFWQTPTVSMGLGPIAAIYQARSWKYLEGRG
                     LMPKTDRKVWCFLGDGETDEPESLGAIGVAGREGLDNLVFVINCNLQRLDGPVRGNGK
                     IIQELEGQFRGAGWNVVKAIWGSYWDPLLAKDTQGILKKLMMETVDGEYQNCKAFGGA
                     YTREHFFGKYPETAAMVANMSDDDIWRLNRGGHDPHKVYAAYDNAMKTVGQPTVILAK
                     TVKGYGMGAAGEALNPTHQTKKLDDSAVRAFRDRFKLPIDDDQFKDGNVPFFRPDEKS
                     PELEYMRERRAKMGGYLPQRRRKATQSLEVPPLATFERLLKSTGEREISTTMAFVQMF
                     NIILRDKQVGPRCVPIVADEARTFGMEGLFRQLGIYAP"
BASE COUNT         2542 a         5072 c         5232 g         2283 t
ORIGIN      
        1 gcgatgcttc tgggcgatcg cgacgatccg ctccagcaac gcgcgcggat agttggcgcc
       61 ggtcgggttg ttcgggttga tcaggacgat ggcgcgggtg cgtgaggaca ccagcgactc
      121 gatctggtcg ggatcgggca ggaagccgtt ggcctcgtcg cagcggtaat acaccggccg
      181 cccatcgttg aggatggtgg ccgcgctcca cagtggatag tccggcgacg gcagcagcac
      241 ttcctcgccc ggattgagca gggcgcgcag ggagatgtcg atcagctcgc tgacgccatt
      301 gcccacgaac acgcgttcgg gcgatgcgtt cggggtacca cgggccttgt ggaaggcggc
      361 gatggcctcg cgcgcttccg gcaggccctg ctggtgggta tagggatcgg tattggcgat
      421 gtggtctgcg atcgcgcgtt gcaggtgatc cggggcacgg aaaccgaagg cgcccgggtt
      481 gccgatgttg agcttgatca ggctgcgtcc ctggccttcc agttcgcggg cacggcgggc
      541 gagctcgccg cggatctcgt agcggacttc gctcaggcgg gtgcgggtgg cgatcttgtg
      601 gcgggggttg aacgacgaca tcgcggctgg gggctggaaa acgaggctgc aagcctagcg
      661 gaaacaggcg tggggcgctt tgagcccctc tcccctcggg agacgggttg ggggtgaggg
      721 tccgttcgag cgctaccccg acacgcactt cgaaccctca tccgcccttc gggcatgttc
      781 tcccggaggg agaacggaca gcctagaatc cgcccatgac ggccatcctc cgcgcatgac
      841 tcccttgcag gccatcggct ggcgctggac gcccgcgacc ctcgacccgg cctgggccgc
      901 gctgatggca gcgaatcccg acgcgcgccc ggcccgcatc accgaacagc atcgcagcgg
      961 gtatgcggtc tcggaggcga tcgacgtcgc ccatcgcgcg gagtcgctgc cggaatggca
     1021 gcgtgccggc agctatcgaa agggtgagat caaccccgaa tcccgaccgg cggtcggcga
     1081 ctgggtgctg gtcgagggcg aggcgccgaa cgccctgcgc atcgtcgccc tgctgccgcg
     1141 cttctccgcg atcaagcgcg gcgcggccgg cgagcattac aagcagcagg tcatcgccgc
     1201 caacatcgac accgtgttcg tggtctgtgg gctggatgcg gacttcaacc cgcgtcggat
     1261 cgaacgctac ctgctgctgg tcggcggcag cggcgtgacg ccggtgatcg tgctgaccaa
     1321 gtccgacaag gacggcgccg atgtcgaggc cgcgttgcat gaactgcggg ccttcggcac
     1381 cccggtgctg gcgttgaatg cgaaggaccg cgacagcgcg gcggcgcttg cgccgtggtt
     1441 gggcgatggc cagagcatcg tgctggtcgg cagttccggc gcgggcaagt cgaccttgac
     1501 caataccctg ctcggcagcg agcggatgaa gaccggcgaa gtgcgcgaga gcgatgcgcg
     1561 cggccgccac accaccaccc atcgcgcgct gatcgcgttg ccgtccggtg cctgcatgat
     1621 cgacacgccc ggcatgcgcg agctcaagcc gaccggcgag gaagtggtgg cggaaagctt
     1681 cgccgacgtc gaagccctcg ccgcgcaatg caagttcaac gactgcaagc acctgaagga
     1741 accgggttgc gccgtgcgtg cggcgatcga gcgcggcgag gtcgacgcgc agcgcgtggc
     1801 gaatttcctc aagctcagcg ccgaggtcgc cggcgccgcc aaccaactcg cgacgcggct
     1861 cgcgcagaag gccgatgcca aggtgcagaa caaggccctg tacaagcgcg tggacgaaaa
     1921 atatggacgg cattgagcgc ttgccccctc tcccgctggc gggagagggt tggggtgagg
     1981 gtggcgatgc gccgagtcac cctcatccgc cttcggcacc ctcagtcgct cggcgcgcct
     2041 tcgatcccgc cagcgggaga aggaaaaagc caaggcatgc cgcatgaacg ctgaaatcgc
     2101 ccaccacgct gcgctcgacg cgcggatggc gaaggccgca cgcgggatcc gcctgctcag
     2161 cctggtgagc tggccggcaa gcgagcagca gacgttcctg gccgacctgg cccgtggcaa
     2221 tgcgcgcctg cccgcgcacc agtatccgac gcacgatttt tcagacgcgc gacgcgagtt
     2281 cgacgcgatc gccgccgatg ccgacccgtc gcatccgctc ggcatctatc tcatcgagtc
     2341 cgcgcacagc tggagccttg ccgccgaatt gctggagagc ctcggnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnngcggc gttctccgac tacgaccgcc accagttgct
     2521 gcagcacgag gccttcgtgc attcgctgac tgccttgaac ggccagcagc aagcggtctt
     2581 gccgagcctc tcgctgtcct cgccacgcac caccgcgacc caggaggggc tggcggtatt
     2641 cgccgagcag atcaccggca gcatcgacat cgagcggatg aagcgggtca gcctgcgcat
     2701 cgaggcggtg gcgatggcgc tggaaggcgc cgacttcctg caggtgttcc gctacttcca
     2761 ggacgcgggg caggacgcgg tggagagctt cacctcggcg cagcgggtgt accggggcgt
     2821 gccgaccagc ggcggtagcg cgttcaccaa ggacaccgtc tacctgcgcg gcctgatcgg
     2881 cgtgcatacc ttcttccgct gggcgctcaa gcacgacaag ttgcgcctgt gccggatgct
     2941 gttcgccggc aagatgaccc tggccgacgt gcagcgcttc gagccgttgt tcgacagcgg
     3001 cgacctggtc gccccgcgct ggttgccgga atgggtggcg cgcgcgaacg gactggccgg
     3061 gatgctggcg ttctcgctgt tcgccaaccg catccgcctg gagcagatca tggatgccga
     3121 aaccgtggtc gacctctgac cccgatgcag ccgaccctcg ccgtcccacc acctgtcgtg
     3181 ttgccgtggt atcggcacct gtacgtgcag gtgctggtcg cgatcgcgct gggggcgctg
     3241 ctcggacatt tcgcgcccgg caccggcgag gcgatgaagc cgctgggcga tgccttcatc
     3301 aagctggtga agatgatcat cggcccggtg atcttcctga ccatcgtcac cggcatcgcc
     3361 ggcatgaccc acctgcgcac ggtcggccgg gtgttcggca aggcgatggc gtacttcctg
     3421 ttcttctcca cgctggcgct ggtggtcggc ctggtagtgg cgaacgtggt gcagcccggc
     3481 gccggcatga acgtcaacgt ggccgacctc gaccggtccg tggtcgatgg ctacgtcagc
     3541 aagtcgcatg agttgacgct ggtcggcttc gtgctggaca tgatcccgga cacgctggtc
     3601 agcgcgttca ccggcggcaa catcctgcag gtgttgctgg tgtcggtgct gttcgggatc
     3661 gcgctggcgc tgtccggcga ccgcgggcgg ccgatggccg acttcctgga atcgctgacc
     3721 atcccggtgt tccggttggt gcggatcctg atgcaggccg cgccgatcgg tgcattcggt
     3781 gcgatcgctt tcaccatcgg caagtacgga ctgggcgtgc tggtgcagct cggctggttg
     3841 gtcgggagtt tctatctcac ctcgctgctg ttcgtgctgg tgatcctggg cacggtctgc
     3901 cggctgtgcg ggttctcggt gctcaagctg atccgttacc tcaaggccga attgctgctg
     3961 gtgctgggta cctcgtcgtc ggagtcggcg ttgcctgcgt tgatggagaa gatggagcac
     4021 gcgggttgcg gcaagcaggt ggtcgggctg gtggtgccca ccggctattc gttcaacctc
     4081 gacggcacca acatctacat gaccctggcc gcgctgttca tcgcccaggc gacgaatacc
     4141 gaattgaccc tcggccacca ggtcacgctg ttgctggtgg cgatgttgag ttccaagggc
     4201 gcggccggcg tcaccggcgc gggcttcatc acactggcgg ccacgctgtc ggtggtgccg
     4261 gaggtcccgg tcgcgggcat ggcattgatc ctgggcgtgg accgcttcat gagcgagtgc
     4321 cggtcgctga ccaacttcat cggcaatgcg gtggccaccg tggtggtcgc gcgttgggaa
     4381 aacgcgctgg atcgcacgcg gctggccgcc gtgctggatg gccaggcggt cgctccgatc
     4441 gcgccgctgc atccagtgcg cgcgatgggc cactgacccc ggtcagcgcg tcgcttgcag
     4501 gctgccgtcg ggctcgacga ggtagtccgc cgcttcgccg tccgccgcga tgcgcacgcg
     4561 gtcgccgatc gcgatgccat catggcgcgg gatgtcgtgg gtcagccgct gcacgatcca
     4621 cggcttgatg ccggggccga tgcggaccac caccgggaaa ggttgcgcgt gggttcgcac
     4681 cgcgtcggcg agcgcgggcg agaacaccga ctggatgcgg ccgacctgct gcatgcgcca
     4741 catcactgcg ccgccgtgca acacagtgag caaggcgaag atgccgatgg cgatgcgacc
     4801 cctgcgcgat gccagcggcc atgtcgcggc gatcgtcatc gcggcgatgg cggcgaagcc
     4861 gtaggcgtag tggttccagg cgctggccag cggcagtacg ggcagcaatg cggcgatgcc
     4921 acccagcagg aacaacgcgg cgaaacgccg acctgcttgc cagagcgccg caagcaggcc
     4981 cagccacgac aggcctgcga ccaacgcagg cagcggtcgc tgcagcgtgg tgatcgcttc
     5041 gtgcagcggc acgatgggca ggaacagctg gtactcgagc caccgctgcg gcacatgtgc
     5101 caacgacagc gtgtactgcg cgccttcgcg cggcgcatgc agcagcacgt cgaggcgcag
     5161 gccgaggtag agcgcggcga ccgcaccgga ggcgagcatc gcccagcacc atttcggctt
     5221 gcggccgtcg aaccaccacg ccagcgcaag cagcggcggg atcgcaaagg cggcctcctt
     5281 cgccagcaat gcgattgcgg tgagcagcgc ggcggccaac gcagcaggaa aagcccgtcg
     5341 cacgtgttgc acccacagcc cgagcaacag tgcgcacccg acccacagca ggtcggcgat
     5401 gcatccgacc cagccatgcg tgtacaccgc gtatggcgac agcacgaagg caagcgcgcc
     5461 gatcaccgca ggacgcgatg ccatcccgaa cccgcgaccc accgcgaaca gcaacgtcgc
     5521 attgagtgcg cccgcggcga ccagcaccgc atggaacagc atcggggtgt cgaacagtgt
     5581 gcgcgacagc cgcatccaca ggttgaaggt cagcggccgg tactggaacg ccgagacgtc
     5641 cagccacggc acccgcacac catcgacggc gtggaccgcc cactgcaatt cgtcgtggct
     5701 gtagtagccg ggattgagga caagcggcag ttgcagcagg caggccagcg cgaacaccag
     5761 cacgggcatc accatcggct tgggggggcg cgaggtcgcc atcacgcgtc ctgcagcaga
     5821 ttcgcggcgc gttcgccact gcccatcgcc agcgtccaac ccaacatgcc gtggccgata
     5881 ttgagcacca ggccgtcgcc tgcccgacgg atgatcgggc tggaccacgg cgtcatcggg
     5941 cgcaggccgg cccagtcgct ttcgatggcg tcgtagtcgg cggcctgcgg aagggattcg
     6001 cgcgccatcg ccaccaggtc tgcgacgcgc gcgcgatccg gcgcggcatc ccaatgattg
     6061 aggtcggcga gcccggcgac ccggatcttg tcgccgagcc ggcagaacac cagcttgcgc
     6121 gcggtatcgg tgatgctggc cgaaggcgcg ttcgcaccgc agggcgcggt gaacgaatag
     6181 ccctttaccg ccatcaacgg cacccgcacg cccggcggcc ggagcagcgc cctgctggcg
     6241 atgccggcgc agacgatcac gttcggcacg gtgacgacgt cgccattgcg cgcatgcaac
     6301 cgccacagcg cgcgttcgcg ttggaggtgt gccaagtcga cgccgaaacg cgcctcgacg
     6361 ccgtagtgcg cgcgcaagac gtccagcaag ccgctgctga atcgccacgg atcgccgact
     6421 tcctcctccg gcgaatggac cacgccggcg atgccgcgtg cgccagccag cgcgggttcg
     6481 atggcgatgg cggcatcggc atcgagcaac tgctgccgca cgccgaaggg tcgcttgcgt
     6541 tcgatcatcg cccgcgccgc cggcaacgat gtcgcgttgt agtagacatg cagcttgccg
     6601 gcgacgcgat ggtcgaagtc gagcggatag cgcatcagca gttccgccat cgccgcgcgc
     6661 gattccagcg cgagttccag cgtggccagc gtgttggccc gcagccgtgc ctgcgtcgca
     6721 ttgcgcagca aggccaggac ccagcgccag aagccgggat ccgtcgagac ccgcgtgcgg
     6781 aacgcgcggt cgcggccggc gagcatgccg ggcagttgct tccacaaggc cggcccggcc
     6841 atcgcatcgg tgtaggcgta gctcagctgc gcgccgttgg cgaacgaggc ctgcagcgcc
     6901 gggccgggcg cgcgatcgat cacgcagatg cgcaggccgc gacgcgcaag cgcatacgcc
     6961 gtcgcgacgc cgacgacgcc agcgccgatc accgcgacat cggcatccgc cgtggcatgt
     7021 gcatgctgcg tcatgcgtgg atcaatacgg ccagaaattg aggaaaccgg tgatgatcag
     7081 cgcattgttg aagtcgatga agaatgcgcc caccagcggt gcgaccagga aggcgcgcgg
     7141 tgccgcgccg tagcgttgcg ccagcgcgcg catcaccgcc atcgcattcg cggtggtgcc
     7201 cagcatgaag ccgatgaagc cgccgcccat caccgccgcg tcgtagtcgc ggcccatcag
     7261 cttgaacacc gccaccgtga acagcaccac cagcgccacc tgcacggcca ggttgaccag
     7321 cagcggcagg cccatgccgg ccagttccca gagcttgagg ttcatcagtg ccatcgccag
     7381 gaacagggcc aggcagatgt tgccgagcag gtccagcgtg tgcacgggca gccgcagcca
     7441 gtcggtggcg tcgtcgaggt tgcgcaccag cgcccctacc agcatgccgc cgatggtcgc
     7501 cggcatggtc atgttgatcg cggcgaatcc cttcatcacc caaccgccgg cccacaccac
     7561 cagcaacagc acgacgatcg ccttgagggc gttgaactcg cgggcagcgt cggtttccgt
     7621 gtgcgccacg cggccttcca cgtcatcggc gacgggcgtc ggggcggcgc ccggggatgg
     7681 cgaaccggtg atgccgtagc ggcgcatcag cacggtgatc gccggcccgc cgatcacgcc
     7741 cccgcagacg atgccggcca tcgcggcggt caccgcgatc gactccgcgc cgcgcaggcc
     7801 ggccgcctcg aacgccggtg cgaaggcgag gccggtcgcc gggccgccgg tcagcgtggc
     7861 ggagccggcc agcacgccga acatcggatg caggtcgaac agccacgcca cggtgatgcc
     7921 caccaggttc tgcaccaccg cgaagaacgt ggccagcagc aggaacagca ccaccggcca
     7981 accgctggcc ttgagcaggc ggaagctggc gttgatgccg accgtggcga agaacgcgac
     8041 catcagcggc gattgcagcg tggtgtccag ctcgaacagc ggcgtttcgc gtgcgtgcgc
     8101 ccaccatgcc gccagtgcca ccagcaggcc gccgaccacc ggttccggca ggttgtagcg
     8161 gcgcagcacc gggatggcgc ggcacagcgc gtagccggcg aacagcgcaa ggccggcgaa
     8221 ggccacggtc tggacgatat ccagctggat catgcgcagg gccccgttcg aatcgacgga
     8281 atgtctcaca ggtcggcccg gcggggccac ggcggctacc gccgcatgcg ggcgcgtaga
     8341 atggacggcc cgtcatggtg gctgcacgcg atgtccgata tcaccggcaa cgacgacctg
     8401 caaaaagacg acctgaagca ggccgcgctc gactatcact cgctcgaacc gaagggcaag
     8461 atcaaggtca ccgcgaccaa gccgatggtc acccagcgcg acctggccct ggcgtactcg
     8521 ccgggcgtgg ccttcgcctg cgaggcgatc gccgcggatc cgcggatggc cagcgagtac
     8581 accgcgcgcg gcaacctggt ggcggtgatc accaatggca ccgcggtgct cggcctgggc
     8641 gacatcggcc cgctggccgg caagccggtg atggaaggca agggcgtgct gttccagaag
     8701 ttcgccggca tcgacgtgtt cgacatcgag ctcaacgagc gcgacccgga caagctggtc
     8761 gacatcatcg ccgcgatgga gcccacgttc ggcggcatca acctggaaga catcaaggcg
     8821 ccggagtgct tcatcgtcga gcgcaagctg cgcgagcgga tgaacatccc ggtgttccac
     8881 gacgaccagc acggcaccgc gatcatcgtc ggcgcggcga tcctcaacgc gttggaagtg
     8941 gccggcaaga agatcgagga cgtgcgcctg gccaccgccg gcgccggtgc cgcgggcatc
     9001 gcctgcctgg acatgctggt cgcgctgggc ctgaaggccg agaacatcct cgccttcgac
     9061 cgcgagggcg tgctctacac cggccgtggg cagatggatc cggacaaggc gcgttacgcg
     9121 cgcgacacgg ccaagcgcac cctggccgag atcgtggacg gcgccgacat cttcctgggc
     9181 ctgtccgccg gcggcgtgct tgcgcctgcg atggtggcga cgatggcgcc gacgccgatc
     9241 atcctggcgc tggccaatcc gtatccggaa atactgccgg aacacgccaa ggcagtgcgc
     9301 ccggactgca tcatcgccac cggccgttcg gactatccga accaggtcaa caacgcgctg
     9361 tgcttcccgt acatcttccg cggcgcgctg gacgtgggcg cgcaggaaat caacgaagcg
     9421 atgaagctgg cctgcgtgcg cgcgatcgcc gcgctggcgc gcaaggaagc ctccgacctg
     9481 ggcagcgcct acggcggcga agtgccgaag ttcggtgccg aatacctgat cccgcgaccg
     9541 ttcgacccgc gcctgctgac catgctggcg ccctgcgtgg cgcaggcggc gatggacagc
     9601 ggcatcgccg agcggccgat cgccgacatt gccgcgtaca aggaaaagct gggccagttc
     9661 atccaccgca ccagcctgat gatgaagccg gtgtacgagc gtgcgcgcag cgagcgcaag
     9721 cgcgtcgcct atgccgaggg cgaggaatac gtggtgctgc aggcggtgca gacggtgatc
     9781 gacgaaggcc tggccttccc gatcctgatc ggccgcccgg aggtgatcga gaaccgcatc
     9841 gccaagctcg gcctgcgcat gcgcgcgggc gtggacttcg agctgaccaa catcaacgac
     9901 gatccgcgct tcgacgacta ctggcggcag taccacgcgc tgaccgagcg tcgtggcgtg
     9961 acgcccgatg cgtccaagaa cctgatccgc tcgcggccga cgatcatcgc cgcgctgatg
    10021 gtcgagcgcg gcgaggccga tgggctgatc accggcatcg ttggccgcta ccacaagaag
    10081 ctgggttacc tgcgcagcat cttcgacttc gataccggcg tgcagggcac cgcggcgatg
    10141 accggggtga tcaacgacaa gggcgcatgg ttcttcgtcg acacccatgt gcagatggat
    10201 ccgtccgccg agcagatcgc cgaggcgacg atgcaggcgg cgtaccggct caagctgttc
    10261 ggcatcgagc cgaaggtggc gctgctgtcg cattccaact tcggcagcca ccaggacgcc
    10321 agtgccacca agatgcgccg cgcctacgag atcgtccgcg cgcgtgcgcc gaagctggag
    10381 atcgatggcg agatgatggc ggacaccgcc tgggacgagg cgctgcgcca gcggatcatg
    10441 cccaacacca cgctgagcgg ccgcgccaac ctgctggtga tgccgaacct ggacgccgcc
    10501 aacatcggct acaacctgat ccgcgtggca accggtggcg tggcgatcgg cccgatcctg
    10561 atgggcctgg accagccggc gcacatcctc acgccggcct cgtccagccg ccgcgtggtc
    10621 aacatgaccg cgatcgtcgc ggtcgaggcg cagatccggg cggcgatgac caccgccgct
    10681 tccggcaagt agcgcaggcg caggcgcgcg gcgggccggg cccgctcagg aaccgccctg
    10741 gatccctgcg agcagggcgg gcagccggtg caactcggac ttgcccagca cggtcgccgg
    10801 gaccggcggc gcgggcgcgt cgtcggcgcc ggtcagcaac accagcggca gcgtgggcag
    10861 gcgttcgtgc agcagcgaca gcgcggccag gccggaatag cccggcaggt tcaggtcgct
    10921 gaccgctacg gtcggttgga acgtctccag ggtctcgcgc agcatgcgtt cgctgctggc
    10981 gcagcgcatg tcgaaggaca ggccggcatc ctccaattgg aagcgcagca ggtctgcatc
    11041 gtcggtgcag tcctcgacca gcaggatgcg cagtgccggc atcggcttac tcggagggcg
    11101 tctcgttgag gaccgcccag aagcggccca gggtcttcac cgcggtgaag aactgctcgg
    11161 aatcgaccgg cttgaccacg taggcgttga cgcccaggtc ccagctgcgc gccaggtcgc
    11221 tctcttcgcg cgacgaggtc agcacgacca ccggcaggcg cttcagctgt tcgtccgcgc
    11281 gcagctggcg cagcacttcc aggccgtcca tgcgcggcat cttgatgtcc agcagcagca
    11341 cggcgggcag gccctcgggg cggtcgctga acttgccgcg gcgatgcagg tagtccatcg
    11401 cctccaggcc gtccatgacg tggacgatgg gattggcgag gtgggcctcg cgcagcgcgt
    11461 cgatcgccat ctcggcgtcg gcggggctgt cttcggcaag caggatggaa cggatggtcg
    11521 gggtcatgcc ttggtgtccg gttgcggtgc gtcggtcgac gcgggaaggg tgaaggcgaa
    11581 gcgcgcgccc ttgccgggcg tggcgtcggc ggaaatatgg ccgccgtggc gggcgagcac
    11641 gcggcgcacg ctggccaggc cgatgccggt gccttcgaat tcgctgatcg aatgcaggcg
    11701 ctggaacacg ccgaacagct tgccggcgta ggccatgtcg aagccgacgc cattgtcggc
    11761 cacctcgaag cgatagtcgc catggtcgtc gcgcgagtag cccacttcga tcaccgcggg
    11821 ttcggagccg ccgctgtact tgaccgcgtt cgagagcagg ttcagccaca cctggcgcag
    11881 catgttctcg tcgccgacca tgatcggcag cggggcgatc ttccactcga tgcggtgccc
    11941 ggggcggtcg tgcgcgttgt tggcgtccag cagcgcgcgg gtttcgtcga ccaccgactg
    12001 catgtccacc ggctgcaggc gcagggcgct gcggccgagc cgcgagtaca ccagcaggtc
    12061 gtcgatcagc tgcgacatcc gccgcgccga ggtggtgatg gtattgaggt agtggaaggt
    12121 cttctcgtcg ttgcggtcgc ccaggtggcg gcccagcttg tcggcgaaac cgccgatgtg
    12181 gcgcagcggc gcgcgcaggt cgtgcgacac cgaatagctg aaggcttcca gttcgcggtt
    12241 gacctcggac acctggtcga ccttgccgat cagctggcga ttcaattcgc gcacctgcag
    12301 ctcggcggcc ttctgcgcgg tgacgtcgtt ggcggtgacc agggcgacgt cgtcgctgcg
    12361 gtcgggcagg gccatccgcc gcgcattgac cagcaggctg cgctcgcggc cgtcctcgcc
    12421 ccggtgggtg atctcgtggt cccacaattc gcggtcgcgg gccagcacgt cggccagccg
    12481 ctggcgggtt tccggggctt cccaggtgcc gccgccgaag gcgccgatcg gcgtgccggg
    12541 ttccgcgtcc gtcgccgcga acacttcgcg gaacgccggg ttgtgcagca ccacccgcag
    12601 gtcgcggtcg accaccgcca tcggctcgcg cacggtctgc agcaccgaca gcgcgcgcgc
    12661 gctggagcgt gccgccgccg cctcggccgc gcgccggttg cgccattgca ggcgcagcag
    12721 caccgccact gcgcccagca gcagcaactg cagcagcatg gcgagccagg tcagcagggc
    12781 gctgcgctcg gacaggcgtg acgcctcgct ggtgcggtcc ttcagcaacg cctgctcgtg
    12841 gtcgaggatc tccgcgccga tgtggcgcag cgggttgcga tccagcagcc actcgatgtc
    12901 ccgcgggtcg gcctgtccgg ccggcttggc caggacggtc tccatgatca tcccgcgctg
    12961 ttcgatgatc gagccgagct tgccgatcct gacctgttgc tcgggattgt cgcgggtgag
    13021 ctgggtcagc cgcgccatgt tgaccgggat ttccttctgg ctctcggcca ggcgctcgcg
    13081 gatcgccggc gagtcatggc cataggcata ggccacggtc gctgactcgc ggttgcgcag
    13141 gtcgtacatc aacccctgca cggtcgactc gatctcgtgc gtgtggttga cgatcgcggc
    13201 cgcatccatg ctcttgcggt tggcttcacg gaccacccag aacggaccga ccacgatcag
    13261 catcaccagc gccccgatca gggcgatcag gccgacggac tggcggatgg gggcgggcgt
    13321 ttgtggcatg ggtgcaggtt cggcgggaaa ggacggcagc agggccgcgg ccggcgggtc
    13381 cccgggccgc cggcgtcgcc acggaacggc acgcccgggg aggatagcca gcccccgtgt
    13441 gaaggcatgc tcacgcggcc gtcacaaacg cgaacgcagc tggaaatgaa ttacccgggc
    13501 aaatcatgat tttgggtgca acgcagcatc gaatcgcccg gaacgcttgc tagactcggg
    13561 ctccgaccga tcgcgccctg gtggaacacc gatgaactgg ctgaacgaga tgctgcagaa
    13621 cgaccccgat ccgcaggagt cgcgggagtg gatcgaatcg ctgaaggcgg tcatcgacca
    13681 cgacggcgcg ggacgcgcgc accagttgct ggaggggatg gtcgaactga cccgccgcgc
    13741 gggcgcctac ctgccgttcg accccaccac cagctacatc aacaccatcc ccacccacat
    13801 ggaaccggcg atgccgggcg acgccaccat ggagtggcgg atccgctcga tgatccgctg
    13861 gaacgcgatg gcgatggtgg tccgcgccaa ccgcaagccg ggcgagctgg gcggccacat
    13921 cgccagcttc gcgtcctcgg ccaccctgta cgacgtcggc ttcaaccatt tctggcgcgc
    13981 ccccagcgcc gaccacccgg gtgatttgct gtacatccag ggccacagct cgccgggcat
    14041 ctacgcgcgc gccttcctgg aaggccgcat cagcgagtcg cagctggaca acttccgcat
    14101 ggaagtggac ggccgcggca tcagcagtta tccgcacccg tggctgatgc cggacttctg
    14161 gcagaccccg acggtgtcga tgggcctggg cccgatcgcc gcgatctacc aggcgcgcag
    14221 ctggaaatac ctggaaggtc gcggcctgat gccgaagacc gaccgcaagg tgtggtgctt
    14281 cctgggcgac ggcgaaaccg acgagcccga atccctcggc gcgatcggcg tggccggccg
    14341 ggaaggcctg gacaacctgg tcttcgtcat caactgcaac ctgcagcgcc tggacggccc
    14401 ggtgcgcggc aacggcaaga tcatccagga gctggaaggc cagttccgcg gcgccggctg
    14461 gaacgtggtc aaggcgatct ggggcagcta ctgggatccg ctgctggcca aggacaccca
    14521 gggcatcctc aagaagctga tgatggaaac cgtggacggc gagtaccaga actgcaaggc
    14581 cttcggcggc gcgtacacgc gcgagcactt cttcggcaaa tacccggaga ccgccgcgat
    14641 ggtggccaac atgtccgacg acgacatctg gcgcctcaac cgcggcggcc acgatccgca
    14701 caaggtctac gccgcctacg acaacgcgat gaagaccgtg ggccagccca ccgtgatcct
    14761 ggccaagacc gtgaagggct atggcatggg cgcggccggc gaggcgctca acccgaccca
    14821 ccagaccaag aagctggacg acagcgcggt gcgcgcgttc cgcgaccgct tcaagctgcc
    14881 gatcgacgac gaccagttca aggacggcaa cgtgccgttc ttccgcccgg acgagaaatc
    14941 cccggagctg gaatacatgc gcgagcgccg cgccaagatg ggcggctacc tgccgcagcg
    15001 ccgccgcaag gccacgcagt cgctggaggt gccgccgctg gcgaccttcg agcgcctgct
    15061 gaagtccacc ggcgagcgcg agatcagcac caccatggcc ttcgtgcaga tgttcaacat
    15121 catcctgcgc gacaagcagg tgggcccgcg ctgcgtgccg atcgtcgccg acgaggcgcg
    15181 caccttcggc atggaaggcc tgttccgcca gctcggcatc tacgcgccg
//
LOCUS       DAOOCX010000054        15074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3579_length_15074_cov_7.587323, whole genome shotgun sequence.
ACCESSION   DAOOCX010000054 DAOOCX010000000
VERSION     DAOOCX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15074
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3579_length_15074_cov_7.587323"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1051
                     /locus_tag="PK399_06725"
     CDS             350..1051
                     /locus_tag="PK399_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPW12846.1"
                     /translation="MEPEPKALADILLALENASRATARPAMARLLAVSKTRSPTEIAA
                     LAASGQCAFGENYVQEALPKIDALRSLGLEWHLIGHLQSNKADLAARAFDWVQSVDRP
                     KLASALARGRPADLPPLNVLLQVNIDDEASKHGCRPPDVDALADAVAAEPRLRLRGLM
                     AIPAPHEDMARRREAFRAMRTLFDGLQARHPGIDTLSMGMSEDFVPAIEEGATMVRIG
                     SALFGARPPKQENNA"
     gene            1048..1866
                     /gene="proC"
                     /locus_tag="PK399_06730"
     CDS             1048..1866
                     /gene="proC"
                     /locus_tag="PK399_06730"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPW12847.1"
                     /translation="MSDTIAFIGGGNMARSLIGGLIAQGRDPATIHVAEPVPSLREAL
                     AVDFGVAVFEDGADAVAGASTWVFATKPQVLRAVCEALATQAQAKQPLVVSIAAGITA
                     AQLERWLGGGIAVVRTMPNTPALLGAGVTGLFASERVDTQGRDFAEALLAAAGKTVWI
                     DDEAQMDAVTAVSGSGPAYVFLLAEAMVDAGIGEGLPRDAARTLTLQTVLGAARMLTE
                     SDVDAAELRRRVTSPNGTTQAAIETFEAGGLRALVADAIHAARVRGAELSAANE"
     gene            1875..2333
                     /locus_tag="PK399_06735"
     CDS             1875..2333
                     /locus_tag="PK399_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HPW12848.1"
                     /translation="MRLLPLLLVFALAACSGGNAPVPAKPHASATASTTVGGATLQTS
                     TVALADLNAAVATRYGIDPTADGVMLLVTVRDAAGNGIDPGDLQLTGTGSVLPDPPKP
                     LELRVVQTDGMTDYLGVVQARAPATVAFKLTAVRGGSRADIATTAELQPR"
     gene            complement(2403..3218)
                     /locus_tag="PK399_06740"
     CDS             complement(2403..3218)
                     /locus_tag="PK399_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009600002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HPW12849.1"
                     /translation="MSASTVGPRRIVCLTEEPTEVLYALGEQNRIVGISGFTVRPPIA
                     RREKPKVSAFTSAKIGEILELKPDFVIGFSDIQADIAAVLIRHGVEVWISNHRSVPGI
                     LDYIRRLGALVGAADRVDAYVDGLRRGLDAIEREAALLPRRPRVYFEEWDEPPITGIR
                     WVAELVRIAGGDDIFPERALEPLAKARILEDADEVVRRAPDIILGSWCGKKFRPEKVA
                     ARPGWDTIPAVRDGQLFEVKSPLILQPGPAALTDGVGGIARIIRAWSEEKGPE"
     gene            complement(3215..3814)
                     /gene="pdxH"
                     /locus_tag="PK399_06745"
     CDS             complement(3215..3814)
                     /gene="pdxH"
                     /locus_tag="PK399_06745"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010381128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HPW12850.1"
                     /translation="MTDLLAPDLLATFDALFAEAEAAGEPDPTAMTLASADAGGRPAA
                     RTVLLKAHDARGFVLYTHLDGRKGRELQANPQAALLFHWPRVRDGVQVRIEGEVEIVA
                     DDEADAYFASRPRGSQVGAWASLQSETLASRDTFEARIARFEQEFEGREVPRPPRWGG
                     FRVMPRAVEFWYGAQYRLHERWLHERGADGTWSKRMLYP"
     gene            complement(3865..4068)
                     /locus_tag="PK399_06750"
     CDS             complement(3865..4068)
                     /locus_tag="PK399_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPW12851.1"
                     /translation="MSVAKIIELNAASKASMEDAVRHGLQKCAESVKNIKGAWVNEIK
                     VVTDEEGNVQEWRVNLRVSFIVN"
     gene            4164..4724
                     /locus_tag="PK399_06755"
     CDS             4164..4724
                     /locus_tag="PK399_06755"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPW12852.1"
                     /translation="MRNDVLVPRHVCSRHVVLVGPMGSGKTTLGRALALRLGLPFVDV
                     DARISADAGCDIPAIFADEGESGFRARESRVLASALEGKPAVIATGGGAVLAEANRSA
                     MRAAGVVVYLQVDPATQLARLAGDAGRPLLDVEDRARRLADLQVIREPLYRDVAHHLF
                     DPSALSPEAAVTALADLLATAEASRE"
     gene            4721..5818
                     /gene="aroB"
                     /locus_tag="PK399_06760"
     CDS             4721..5818
                     /gene="aroB"
                     /locus_tag="PK399_06760"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPW12853.1"
                     /translation="MTVSPRTVDVGGGAPYRITIGPGLLDDGDLMAATLRGRHALIVS
                     DANVAPLYADRLETALRRAKPSLQVARHVVAAGEAEKTLANFATVIEALASLGATRDA
                     SVVALGGGVVGDMAGFAAACWMRGIDCVQIPTTLLAMVDSSVGGKTAVDIPQGKNLVG
                     AFHPPRAVIADTGVLRTLPPRELRAGLAEVIKYGAIIDAPFLTWLDGNAAGLLAGDDA
                     LLTEAIARSCLHKAGITERDPFEHGERALLNFGHTFGHAIEAEQGYAEGLNHGEAVAV
                     GMVLAARLSHALDMATRDDADGLQALLQRFGLPTAIPAWLDADALLARMRLDKKTQAS
                     GLRFILWDRPGAARVVADVDEAAVLHVLRKG"
     gene            5845..6099
                     /locus_tag="PK399_06765"
     CDS             5845..6099
                     /locus_tag="PK399_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HPW12854.1"
                     /translation="MRLLLQQRPDGREAPRFVQLQLQPDLLGGWTLLRETGQIGGRST
                     LKREQYADQASALSALESARDAQLKRGFQLMFAQGADAPR"
     gene            6111..7163
                     /gene="hemE"
                     /locus_tag="PK399_06770"
     CDS             6111..7163
                     /gene="hemE"
                     /locus_tag="PK399_06770"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HPW12855.1"
                     /translation="MPKNDRFLRALRREPVDCTPVWLMRQAGRYLPEYRATRKAAGSF
                     LAMAKNPDLACEVTLQPLRRFPLDAAILFSDILTVPDAMGLGLYFVEGEGPKFERPLR
                     TPADIARLAVPDMEAELGYVMDAVRIIRRELDGSVPLIGFSGSPWTLACYMVEGGGSK
                     DWGKVKALALNEPAAMHQLLSVVTDAVIAYLSAQHAAGAQALQVFDTWGGTLGPAMYR
                     EFSLPYLVRIARALKDLGAPVILFGKGNAPHLDDLFASGADAIGVDWTVTLEDAARRA
                     NGRVALQGNLDPAILYGNADAIRAQVRGALDSYAIGNGGSREGHVFNLGHGMSPDMNP
                     DHVAVLVDEVHAYSRR"
     gene            complement(7192..10704)
                     /locus_tag="PK399_06775"
     CDS             complement(7192..10704)
                     /locus_tag="PK399_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPW12856.1"
                     /translation="MRGIRWLVLLALLACACTLAGVPERPRFRVAGPAQGLPSTDIKA
                     LARDADGYLWIATADGLARHDGIDMRIWRHDPQDPAGLPGNNVQALVVDARNRVWIAT
                     EDGGISVLDARRTRFSHYRLSSHPQLGSDDVWALARQGDAVWAGTYDGGLTRIDVDGG
                     MQRYTLANDGLPSDTVLSLAVDADGVLWVGTDKGLARQRGRRFEPVTLPGADGVPMVY
                     SLSPQADGLWAGTSLGVWTYREGRWSRPDWAPMFYPPNAILAIATDRDGDHWIGTGRG
                     LWRQHGGAAPEPVRTGGVEVPRMINALLAEPDGALWVPVAGRGLGYLRADWRQAARLQ
                     GPADGLQGAMYRTLGGARNGGAWLGGVNGVVERLDRDGTAQPMDADALQRLQRVKPAS
                     MLEDDAGRLWIGHRTGLIRVGSDGAIDEWGTDDPLAPVPSGMISQILLAPDDTLWLAV
                     AGGGVQQRDPATGRILIGVAAGATGGLGAADIDAMVIAPTGEPWVGGSDGVASLDRTY
                     ARFRPVQGLGSERVHALAFDGPDLLWLQRLSGLEQYRRYNAGWKLIDRVDARYGMPAV
                     AAAALLVDARHRVWITSSRGLYRWEPGRRNLRRVGMQDGASSEEYLDRSALLREDGVL
                     LAATADGSLVMVDTTAADPARTRPPLRFDGIAVRRDGEWRELPMDTSLRLGREDRELR
                     IRARLLAFDDPPANRYWSRLEGFERDWVTLGASGERVFTGLAPGHYTLRMRAQDAAGN
                     AAAEQQLVFVVPPPWWRTAWAQAGFVALLLLALFALAWAYRQRLKRKHAWQLAEQKQA
                     LAEQASEAKSRFLATLGHEVRTPMTGVLGMSELLQGTPLDARQRRHVDAIRRAGEHLL
                     RLVNDALDLARIEAGRLELANADFALRPLLDEVAALMAPVAERKGLAFIDGMAPDAPT
                     ALHGDRTRLQQILLNLVGNAVKFTESGHVALETCALVPHGVRFVVADTGPGLDAAQQA
                     RLFRRFEQADGARTALRYGGSGLGLSISQELAAAMGGRIGIESEPGMGTRFIVDLPLV
                     HAEVTATASPSDAPRINAAALRLLLVEDDGIVAEAISGLLQAQGHRVAHAGHGLAALA
                     EVATRAFDAALIDLDLPGMDGLALARMLRAQGHAFPLLAVTARSDADAEVQARAAGFD
                     GFLRKPVTGAMLAQALEALMSNAE"
     gene            10790..11458
                     /locus_tag="PK399_06780"
     CDS             10790..11458
                     /locus_tag="PK399_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPW12857.1"
                     /translation="MLPRRRLPASCLPALLLALACAPTAAGEPPRYAFDPVHTRVMFA
                     IDHAGFSKAIGTISGSQGSLQFDADDWASARLDVLVPMDRLDMGDSGWSASVFAPRFL
                     DVKRYPQARFVATDGMQRGTGNRGRACGQLTLHGVTRPLCLDVVFNKAGRYPLPPFRR
                     TIGFSATATLKRSDYGMTSWQSLVGDDIELRIEAELFRSDADAPAPTQPAPVAGDPAP
                     PPND"
     gene            11476..12060
                     /locus_tag="PK399_06785"
     CDS             11476..12060
                     /locus_tag="PK399_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HPW12858.1"
                     /translation="MPLKNTRDRWGSVSKLLHWTTLLLLVWIGWLGLTMGDLPNGPGK
                     IATYALHKSIGITILTLAAIRIAWRLYAGAPDPIPGSPRWQERIASLTHLALYGLLLA
                     MPLSGWILNSASGFPLQWFGLFNLPAITGKDHELHELAEDIHEWLFWAMATLVVLHAS
                     AAFYHHLFQRDATLARMLPGRASRVEPSMETPDA"
     gene            12053..12595
                     /locus_tag="PK399_06790"
     CDS             12053..12595
                     /locus_tag="PK399_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPW12859.1"
                     /translation="MRKTLLLALALLCAPAWAADYVQAPGSNLTFAGTYQGEVFTGKF
                     PGFVTAFRFDPKQLAASKLQVVIPLATAATSNADYDGELRGGSFFDAARFPRATYIAT
                     TFRALGGNRYAADGTLTLRGISKPVVLEFTWTPGAAPVLSGKATVKRLAFGVGGGDWA
                     DTALIPDAIAISTKVVFKAK"
     gene            12668..13657
                     /locus_tag="PK399_06795"
     CDS             12668..13657
                     /locus_tag="PK399_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW12860.1"
                     /translation="MPLFPLFTDLQGREVLVVGGGEVATRKIHALLRAGARVLIYARE
                     LAPPLQALAVAGHLQRLEGDAVESAWIERAWLVVAATDDHVLNREVAGMAEAAKRWIN
                     VVDDAELSSYQVPAVVDREPITIAISSAGAAPMLARQLREQLEAEIDPSIGELARLFA
                     RHRGRIRERLPLMRERRDWFERILAGDIPVLLRAGNADAAEQAFKDALDEGGTSRVRG
                     SVALVGCGDGDPGLLALRALRLLNQADLVLVGDGVAQAIVDMARRDAAMQPLPSAEDL
                     SEVLSAHVEAGRRVVCLRPGSGFTDAAGRVLRTTLHERGHACETLPGAAWPSD"
     gene            complement(13679..13909)
                     /locus_tag="PK399_06800"
     CDS             complement(13679..13909)
                     /locus_tag="PK399_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HPW12861.1"
                     /translation="MELILYQRDTCHLCDLALAVLAEARAPDFESVFIDDDAALEARY
                     GLRVPVLREHVGGTELDWPFDVDAVRAFLGME"
     gene            13982..>15074
                     /locus_tag="PK399_06805"
     CDS             13982..>15074
                     /locus_tag="PK399_06805"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HPW12862.1"
                     /translation="MAVSTFDLYKIGIGPSSSHTVGPMRAAARFVEHHLAEAGRLDDV
                     ARVRAEVFGSLALTGRGHGTDKAVLMGLEGHWPNRIDPDVIPAALERIRGSKSIRLHG
                     QREIAFNEKSDLVMNKRQKLPFHTNGMRFIAYDANGAEVASRDYYSVGGGFVVNQDEA
                     AEDRIVADTTPVAHPFASGDELLARCAASGLSIAQLMLENERAWRSDDEVIAGLRELW
                     TAMQDCVARGIRETGTLPGGLHVSRRAPSLHAELSGKPEAAMRDPLTVLDWVNLYALA
                     VNEENAAGGRVVTAPTNGAAGIIPAVLHYYDRFCAGASEKGVFEFLLTASAVGILYKE
                     NASISGAEVGCQGEVGVACSMAAAGLTAAL"
BASE COUNT         2330 a         5476 c         5000 g         2268 t
ORIGIN      
        1 acgcggatca tgggcggcag gccggcggac aggtgcaggt ccgacgcctt gttcttgacc
       61 gaaaacgcga gaagctcagc aatatccatg gtggcaacac tccccaggcg cgcggcatct
      121 cccctgccgc gcaagtcgtc gttaccgcac cggggcgctc cagccccgtc gcgacgcttc
      181 gagtcccggg caagcccgtc ctcgcgtgcc agtgcaccga cgccttcgcg gcggaacctg
      241 gcgggtgcag cctccctgca gcacccttga acccgctgcc gcgaacgtac tgtcgcaagc
      301 agggggccgt gggcagtata tgcctcccgc agtcgccgac aatgacccca tggaacccga
      361 gcccaaggcc cttgccgaca tcctgcttgc cttggagaac gcaagccgcg cgaccgcccg
      421 gccagccatg gcgcgcttgc tggcggtctc caagacccgc agtccgacgg agatcgccgc
      481 actggcggca tccggccaat gcgcgttcgg cgagaactac gtgcaggaag ccctgccgaa
      541 gatcgatgca ctgcgcagcc tcggcctgga gtggcacctg atcggccacc tgcaatcgaa
      601 caaggccgac cttgccgccc gcgccttcga ctgggtgcag tcggtcgacc ggcccaagct
      661 ggcctcggcc ctcgcccgcg gtcgccccgc ggacttgccg ccgctgaacg tgctgctgca
      721 ggtcaacatc gacgatgagg cgagcaagca cggctgccgc ccaccggacg tcgacgcgct
      781 ggccgacgcc gtcgccgcgg agccgcgcct gcggctgcgc gggctgatgg cgatccccgc
      841 accgcatgag gacatggcgc gccgtcgcga ggcattccgc gccatgcgca cgctgttcga
      901 tggcttgcag gcgcggcatc ccggcatcga tacgctgtcg atgggcatga gcgaggactt
      961 cgtgcccgcc atcgaggaag gtgcgaccat ggtgcgcatc ggcagcgcgc tgttcggggc
     1021 gcggccaccc aagcaggaga acaacgcgtg agcgacacga tcgccttcat cggcggcggc
     1081 aacatggcgc gcagcctgat cggcgggttg atcgcgcagg gccgcgatcc cgccacgatc
     1141 cacgtcgccg aaccggtgcc ctcgctgcgt gaggcgctgg ccgtcgattt cggggtcgcc
     1201 gtgttcgaag acggcgcgga cgccgtcgca ggcgcctcca cctgggtgtt cgccaccaag
     1261 ccgcaggtct tgcgcgcggt atgcgaggcg ttggccacac aggcacaggc gaagcagcca
     1321 ttggtggtct ccatcgcggc cggcattact gccgcgcaac tggaacgctg gctcgggggc
     1381 ggcatcgcgg tggtgcggac gatgccgaac acccctgccc tgctcggcgc cggcgtgacc
     1441 ggcctgttcg ccagcgagcg cgtggatacg caaggccgcg atttcgccga ggcgctgctc
     1501 gcggctgcgg gcaagaccgt gtggatcgac gacgaagcgc agatggacgc ggtcaccgcg
     1561 gtctccggca gcggcccggc ctacgtgttc ctgctggccg aagcgatggt cgatgccggc
     1621 atcggcgaag gcctgccgcg cgacgccgcg cgcacgctca ccctgcagac cgtcctgggt
     1681 gccgcgcgca tgctgaccga atccgatgtc gatgccgccg aactgcgccg ccgggtgacc
     1741 tcgccgaacg gcaccaccca ggccgcgatc gagaccttcg aagccggtgg cttgcgcgcg
     1801 ctggtcgccg atgcgatcca tgccgcgcgc gtgcgcggcg ccgaactgtc cgccgccaac
     1861 gaatgaggat gccaatgcgc cttcttcccc tgttgctcgt gttcgccctt gccgcctgca
     1921 gcggcggcaa cgcgcccgtg cccgccaagc cgcacgcgag tgccactgcc agcacgacag
     1981 tgggcggcgc gacgctgcaa acctcgaccg tggcgctggc cgacctcaat gccgccgtcg
     2041 ccacgcgcta cggcatcgac cccaccgccg atggcgtgat gttgctggtg accgtgcgcg
     2101 acgccgccgg caacggcatc gaccccggcg acctgcaact gacggggacc ggatcggtgt
     2161 tgccggatcc gccgaaaccg ctggaactgc gcgtcgtgca gaccgacggg atgaccgatt
     2221 acctcggcgt ggtgcaggcc cgggccccgg ccaccgtggc cttcaagctc accgccgtgc
     2281 gtggcggttc gcgcgccgac atcgcgacca cggcggaatt gcagccccga tgaggaaaag
     2341 gggccagagt gaattttgcg ccgagggtgc ttggcaactt cgcatcgcac caacgcaaaa
     2401 cttcactctg gccccttttc ttctgaccac gcacggatga tccgggcgat cccgccgacg
     2461 ccatcggtca acgccgccgg gccgggctgc aggatcagcg gcgacttgac ttcgaacaac
     2521 tggccatcgc gcaccgccgg gatggtgtcc cagccggggc gggcggcgac tttctcggga
     2581 cggaatttct tgccgcacca cgagcccagg atgatgtccg gcgcgcggcg taccacctcg
     2641 tcggcatcct ccaggatgcg tgccttcgcc agcggttcca gcgcgcgctc cgggaagatg
     2701 tcgtcgccgc cggcgatgcg gaccagttcg gcgacccagc ggatgccggt gatcggcggt
     2761 tcgtcccatt cctcgaaata gaccctgggc cgccgcggca gcagggccgc ttcacgttcg
     2821 atcgcatcca gcccgcgtcg caggccatcg acatacgcat cgacgcgatc cgcagccccg
     2881 accagcgcac cgagccggcg gatgtagtcg aggatcccgg gcacgctgcg gtggttgctg
     2941 atccagacct ccacgccatg ccggatcaac acggcggcga tgtcggcctg gatgtcggag
     3001 aagccgatca cgaagtcggg cttcagttcc aggatctcgc cgatcttcgc gctggtgaac
     3061 gcgctgacct tgggcttttc cctgcgcgcg atgggcggcc gcacggtgaa cccgctgatg
     3121 ccgacgatgc ggttctgctc gcccagcgcg tacaggactt cggtgggctc ttcggtcagg
     3181 cagacgatgc gtcgggggcc gaccgtcgat gcactcacgg atacagcatc cgcttcgacc
     3241 aggtgccatc ggcaccgcgt tcgtgcagcc agcgctcgtg caggcggtat tgcgcgccgt
     3301 accagaactc gacggcgcgc ggcatcaccc ggaagccgcc ccagcgcggc gggcgcggga
     3361 cctcgcgccc ctcgaactcc tgctcgaagc gggcgatgcg cgcctcgaag gtgtcgcgcg
     3421 atgccagcgt ttccgactgc agcgacgccc acgccccgac ctggctgccg cgcgggcggc
     3481 tggcgaaata ggcgtccgct tcgtcatcgg ccacgatttc gacctcgccc tcgatccgca
     3541 cctgcacgcc gtcgcgcacg cgcggccaat ggaacagcaa cgcggcctgc gggttggcct
     3601 gcaactcccg gcccttgcga ccatccaggt gcgtgtacag cacgaagccg cgggcgtcgt
     3661 gcgccttcag caacaccgtg cgcgcggccg gtctcccacc agcatcggcg ctggccaggg
     3721 tcatggcggt gggatccggc tcaccggcgg cttccgcctc ggcgaacagg gcatcgaagg
     3781 tggcgaggag gtcgggcgcg aggaggtcgg tcatgcacgc attgtcgcgc gcatgccgcg
     3841 cccgcgcatc tgggccgtga tcgactagtt gacgatgaag ctcacccgca ggttgacccg
     3901 ccactcctgc acgttgccct cttcgtcggt gacgaccttg atttcgttga cccacgcgcc
     3961 cttgatgttc ttgaccgact cggcgcactt ctgcaggccg tgcctgaccg catcttccat
     4021 gctggccttg gacgcggcgt tgagttcgat gatcttggca acggacatgg cgattcctcc
     4081 cggtgatccg gcaaggcgat gccggaatgg gtagcctcct cccgccccgg ttaagcccgc
     4141 gcaacggctg ctagcatgtt gcgatgcgca acgacgtcct tgttccccgc cacgtctgtt
     4201 cccggcatgt cgtcctggtc ggcccgatgg gttcgggcaa gaccacgctg ggccgcgcgc
     4261 tcgcgctgcg gctcggcctg cccttcgtgg acgtggatgc gcggatctcg gcagacgccg
     4321 gttgcgacat ccccgccatt ttcgcggacg aaggcgagtc cggcttccgc gcgcgcgagt
     4381 cgcgggtgct ggcgtcggca ctggaaggca agccggccgt gatcgcgacc ggcggtggcg
     4441 cggtgctggc cgaggccaac cgcagcgcga tgcgcgctgc cggtgtcgtg gtgtacctgc
     4501 aggtcgatcc cgccacgcaa ctggcacgac tggcgggcga tgccggccga ccgctgctgg
     4561 acgtcgaaga tcgtgcccgg cgcctggcag acctgcaggt gatccgcgaa ccgctctatc
     4621 gcgacgtcgc ccaccacctg ttcgatccca gcgcgctgtc gccggaggcc gcggtcaccg
     4681 cgctcgccga cctgctcgcc actgccgagg catcccgcga atgactgttt cgccacgcac
     4741 tgtcgacgtg ggcggcggcg cgccctaccg catcaccatc ggccccggcc tgctcgacga
     4801 cggcgacctg atggcggcga cgctgcgtgg ccgccatgcg ctgatcgtca gcgatgcgaa
     4861 cgtggcgccg ctgtacgccg accgcctcga aaccgccttg cgccgggcca agccgagcct
     4921 ccaagtcgcc cgccacgtgg tcgccgccgg cgaggccgaa aagaccctgg ccaatttcgc
     4981 cacggtgatc gaggccctcg cgtcgctggg ggccacccgc gacgcgtccg tggtcgcgct
     5041 gggcggcggc gtggtcggcg acatggccgg cttcgccgcc gcctgctgga tgcgcggcat
     5101 cgactgcgtg caaatcccca cgacgttgct ggcgatggtg gattcctcgg tcggcggcaa
     5161 gaccgcggtg gacatcccgc aaggcaagaa cctggtcggc gccttccatc caccgcgcgc
     5221 cgtcatcgcc gataccgggg tactgcgcac gctgccgccg cgcgaactgc gcgccggcct
     5281 ggcggaagtc atcaagtacg gcgcgatcat cgatgcgccg ttcctcacgt ggctcgatgg
     5341 caacgcggcc ggcctgctgg ccggcgacga tgccctgctc accgaggcga tcgcgcgcag
     5401 ctgcctgcac aaggcgggga tcacggaacg cgacccgttc gaacatggcg aacgcgcact
     5461 gctcaacttc ggccacacct tcggccatgc gatcgaggcc gaacagggct atgccgaagg
     5521 cctcaaccac ggcgaggcgg tggcggtcgg catggtgctg gcggcgcggc tgtcgcacgc
     5581 gctcgacatg gcgacgcgcg acgacgccga tggcttgcag gcgctgctgc aacgcttcgg
     5641 cctgcccacc gcgatcccgg cctggctgga tgctgatgcg ctgctggcgc gcatgcgcct
     5701 ggacaagaag acccaggcca gcgggctgcg cttcatcctc tgggatcgcc cgggtgccgc
     5761 acgcgtggtc gcagatgtgg acgaagcggc cgtgctccac gtcttgcgca agggataaac
     5821 ccgccacccg gctacaatcg cgccatgcgc ctgctgctgc aacaacgccc cgatggccgc
     5881 gaagccccgc gtttcgtcca actccagctc cagcccgacc tgctgggcgg ctggaccttg
     5941 ctgcgcgaga ccggccagat cggcgggcga agcacgctca agcgcgaaca atacgccgac
     6001 caggccagtg ccctcagcgc gctggaatcg gcccgcgacg cccagctcaa gcgcggcttc
     6061 caactcatgt tcgcgcaagg cgcggacgca ccccgctaac cggattgcac atgcccaaga
     6121 acgaccgctt cctccgcgca ctgcgccgcg aacccgtgga ctgcacgccc gtctggctga
     6181 tgcgccaggc cggtcgttac ctgccggagt accgcgccac ccgcaaggcg gccggcagtt
     6241 tcctggccat ggccaagaac ccggacctgg cctgcgaggt caccctgcag ccgctgcgtc
     6301 ggttcccgct ggacgccgcg atcctgttct ccgacatcct caccgtgccc gatgcgatgg
     6361 gcctgggcct gtatttcgtc gagggcgaag gcccgaagtt cgagcgcccc ttgcgcaccc
     6421 ccgccgacat cgcccggctc gcggtgccgg acatggaggc ggaactgggc tacgtgatgg
     6481 acgcggtccg catcatccgc cgcgaactgg acggcagcgt gccgctgatc ggcttctccg
     6541 gcagcccgtg gacgctggcc tgctacatgg tcgagggtgg cggcagcaag gactggggca
     6601 aggtcaaggc gctggccttg aacgaaccgg ccgcgatgca ccagttgctg tcggtggtca
     6661 ccgatgcggt catcgcctac ctgtccgcgc aacacgcggc cggcgcgcag gcgctgcagg
     6721 tgttcgacac ctggggcggc accctggggc cggcgatgta ccgcgagttc tcgctgccct
     6781 acctcgtccg catcgcgcgc gccctgaagg acctcggcgc accggtgatc ctgttcggca
     6841 agggcaatgc cccgcatctc gatgacctgt tcgccagcgg cgcggacgcc atcggcgtgg
     6901 actggacggt cacgctggaa gacgcggcac gccgcgccaa tggccgcgtt gccctgcagg
     6961 gcaacctcga ccccgcgatc ctgtacggca atgcggatgc gatccgcgcg caggtgcgcg
     7021 gcgcactgga cagttacgcg atcggcaacg gtggctcacg cgaaggacac gtgttcaacc
     7081 tggggcatgg catgtcgccg gacatgaacc cggaccacgt cgccgtgctg gtcgacgaag
     7141 tccacgccta cagccgtcgc tgaggcgacg caggccgccc gggacgccgc cctattcggc
     7201 gtttgacatc aacgcctcca gcgcctgcgc cagcatcgcg ccggtcaccg gcttgcgcag
     7261 gaacccgtcg aagccggccg cccttgcctg gacttcggca tcggcatccg agcgcgcggt
     7321 caccgccagc agcgggaagg catgaccttg cgcgcgcagc atccgcgcca gcgccaaccc
     7381 gtccatgccc ggcaggtcga ggtctatcag cgcggcatcg aacgcacgcg tcgcgacctc
     7441 ggccagcgcc gccaggccat gcccggcgtg cgccacgcgg tggccctggg cttgcagcag
     7501 gccgctgatc gcctcggcca cgatgccgtc gtcctcgacc agcagcaggc gcaacgccgc
     7561 cgcattgatg cgcggcgcat cggacgggga cgcggtggcg gtgacttcgg catgcaccaa
     7621 cggcaagtcg acaatgaagc gggtgcccat acccggttcg ctttcgatac cgatccgccc
     7681 acccatcgcc gccgccagtt cctgcgagat cgacagaccc agcccgctac cgccgtagcg
     7741 tagcgcggtg cgcgcgccat ccgcctgttc gaagcggcgg aacaagcgcg cctgctgcgc
     7801 ggcgtcgagg cccgggccgg tgtcggccac cacgaagcgc acgccatgcg gcaccaaagc
     7861 gcaggtttcc agcgcgacgt ggccgctctc ggtgaacttc accgcatttc cgaccaggtt
     7921 gagcaggatc tgttgcaggc gcgtgcggtc gccgtgcagt gcagtcggcg catccggcgc
     7981 catcccgtcg atgaaggcga ggcccttgcg ttcggcgacc ggtgccatca gcgcggcgac
     8041 ctcgtccagc aatgggcgca gggcgaagtc ggcattcgcc aactccagcc tgccggcctc
     8101 gatccgggcc aggtcgaggg cgtcgttgac cagccggagc agatgctcgc cggcccggcg
     8161 gattgcgtcc acgtggcggc gctggcgcgc atccagcggc gtgccctgca gcaactcgct
     8221 catgccgagc acgccggtca ttggcgtccg tacttcgtgg cccagggtcg ccaggaaacg
     8281 cgacttcgcc tccgacgcct gctcggccaa tgcctgcttt tgttccgcca gctgccacgc
     8341 atgcttgcgc ttcaggcgct gccggtacgc ccacgccaac gcgaacagcg ccagcagcag
     8401 cagggccacg aagcctgcct gcgcccaggc ggtgcgccac cagggcggcg gcaccacgaa
     8461 caccagttgc tgttccgccg ctgcgttccc ggcggcatcc tgcgcgcgca tccgcaacgt
     8521 gtagtgacct ggcgccaggc cggtgaagac gcgttcgcca ctggcgccca aggtgaccca
     8581 atcgcgttcg aagccctcca gccgcgacca gtagcggttc gccggcggat cgtcgaacgc
     8641 cagcaggcgg gcacggatcc gcagttcccg gtcctcgcgg ccgaggcgga gcgacgtgtc
     8701 catcggcaat tcccgccact cgccatcgcg acggaccgcg atgccatcga agcgcagcgg
     8761 cggccgtgtc cgcgccgggt cggcagcggt ggtatccacc atcaccaggc tgccgtcggc
     8821 ggtcgctgcc aacagcacac catcctcgcg cagcaaggca ctgcgatcga ggtattcctc
     8881 gctgctggcg ccgtcctgca tgccaacccg tcgcaggttg cggcgtcccg gttcccagcg
     8941 atacaggccg cgactgctgg tgatccagac ccgatggcgg gcatcgacga gcaacgcggc
     9001 agcggccacc gccggcatgc cgtagcgcgc atccacgcga tcgatcagct tccagcccgc
     9061 gttgtaacgt cgatactgct ccagccctga tagccgctgc agccacagca ggtcggggcc
     9121 gtcaaacgcc agtgcatgca cgcgttcgga acccaggccc tggacgggac ggaagcgagc
     9181 ataggtgcgg tccagcgatg ccacgccgtc gctgcctccc acccacggtt caccggtggg
     9241 cgcgatcacc atggcgtcga tgtcggcagc acccagtcca cccgtggcgc ccgcagcaac
     9301 gcctatcaga attcggccag tggctggatc gcgttgctga acgccgccgc cagccaccgc
     9361 cagccacaac gtgtcatccg gtgccagcaa gatctggctg atcatgcccg acggaaccgg
     9421 tgccagtgga tcgtcagtgc cccattcgtc gatggcaccg tcgctgccaa cccggatcaa
     9481 accggtccgg tggccgatcc agagccggcc agcgtcgtct tcgagcatcg aggccggctt
     9541 cacgcgctgg agccgctgca gcgcatcggc gtccatgggt tgcgccgtgc catcgcgatc
     9601 cagacgctcg acgacgccgt tcacaccacc cagccacgcc ccaccattgc gtgctccgcc
     9661 gagggtgcgg tacatcgcgc cctgcaggcc gtcggccggc ccctgcaagc gtgccgcctg
     9721 acgccagtcg gcgcgcaggt agcccaaccc ccggcccgcc actggcaccc acagcgcacc
     9781 atccggctcg gccagcaatg cattgatcat gcgcggtacc tcgacaccac cggtgcgcac
     9841 cggctcgggg gcggcaccac cgtgctgacg ccataacccg cgccctgtcc cgatccagtg
     9901 gtccccgtcg cgatcggtgg ctatcgccag tatcgcgttg ggcggataga acatcggtgc
     9961 ccaatcggga cgggaccaac gaccttcccg gtaggtccat acgcccagtg acgtgcctgc
    10021 ccacaggccg tccgcctgtg gcgacaacga atacaccatc gggacgccat ccgcccccgg
    10081 caaggtgacg ggctcgaagc gcctgccacg ctggcgagcc aggcctttgt cggtcccgac
    10141 ccacagcacg ccgtcggcat ccacggcaag cgacaacacc gtgtccgatg gcaggccatc
    10201 attggccagg gtgtagcgct gcatcccccc atcgacatcg atgcgggtca gcccgccatc
    10261 gtaggtgcct gcccacacgg cgtcgccctg gcgggccaac gcccatacat catcgctgcc
    10321 gagctgcgga tggctggaca ggcggtaatg gctgaagcgc gtgcgccggg catcgagcac
    10381 gctgatgccc ccatcctcgg tcgcgatcca gacgcggttg cgggcgtcga cgaccagtgc
    10441 ctgcacgttg ttgcccggca atcctgccgg atcctgcgga tcatggcgcc agatccgcat
    10501 gtcgatgccg tcatggcggg ccaagccgtc cgcggtggcg atccacaggt agccgtcggc
    10561 atcgcgggcc agcgccttga tgtcggtgga aggcagtcct tgcgcaggac cggccacgcg
    10621 gaagcgcggg cgttcgggca cgccggccag cgtgcatgcg cacgccagca acgccagcaa
    10681 caccagccat cggattccac gcatgtcggt tctcccccag ccgatgctgg caacgcttcg
    10741 ttgcggacgc aagcgcgccg attccgcgat gcaggcctag aatcgcgcca tgcttccccg
    10801 gcgacgcctc ccagcctcct gcctgcccgc cctgttgctg gcgttggcgt gcgcaccgac
    10861 cgcggccggg gaaccgccgc gctacgcgtt cgacccggtg catacgcggg tgatgttcgc
    10921 catcgaccac gccggcttct ccaaggcgat cggcacgatt tccggcagcc agggcagcct
    10981 gcagttcgat gccgacgact gggccagcgc gcggctcgac gtgctggtgc cgatggaccg
    11041 cctcgacatg ggcgacagcg gttggagcgc atcggtgttc gcaccccgct tcctcgacgt
    11101 gaagcgctat ccgcaagcgc gcttcgtggc cacagacggc atgcagcggg gtaccggcaa
    11161 ccgtgggcgc gcctgtggcc agctcaccct gcacggcgtc acccgtccgc tgtgcctgga
    11221 cgtggtgttc aacaaggccg gccgttaccc gctgccgccg ttccgccgca cgatcggctt
    11281 ttccgccacg gcgacgctca agcgcagcga ctacggcatg accagttggc agtcactggt
    11341 cggcgacgac atcgagctcc ggatcgaagc ggaactgttc cgcagcgacg cagacgcccc
    11401 ggcgcccacg cagcccgccc ccgtcgctgg cgatcccgcg ccgccgccca acgactgaag
    11461 ccaacgagac ccgccatgcc gctgaagaac acacgcgacc gctggggcag cgtcagcaag
    11521 ctgctgcact ggaccaccct gctcctgctg gtctggatcg gctggctggg cctgaccatg
    11581 ggcgacctgc cgaacggtcc cggcaagatc gccacctatg cgctgcacaa gtcgatcggc
    11641 atcaccatcc tcaccctggc cgcgatccgc atcgcctggc ggctatatgc cggggcgccc
    11701 gacccgatcc cgggttcacc gcgctggcag gaacgcatcg cctcgctcac ccacctcgcc
    11761 ctgtatggcc tgttgctggc gatgccgctg agcggctgga tcctcaactc cgcctccggg
    11821 ttcccgctgc agtggttcgg gctgttcaac ctgccggcga tcaccggcaa ggaccacgag
    11881 ctgcatgaac tggccgagga catccacgaa tggctgttct gggcgatggc gacactggtc
    11941 gtcctgcatg cctcggcggc gttctaccac cacctcttcc agcgcgacgc caccctggcc
    12001 cggatgctgc cgggtcgcgc atcgcgcgtc gaaccatcca tggagacgcc cgatgcgtaa
    12061 gaccctgttg ctggccctgg cgttgctttg cgcacccgcg tgggcggccg actacgtgca
    12121 ggcgccgggc tccaacctga ccttcgccgg cacgtaccaa ggcgaggtgt tcacgggcaa
    12181 gttcccgggc ttcgtgaccg ccttccgctt cgatccgaag cagttggccg catcgaagct
    12241 gcaagtcgtg atcccgcttg ccactgccgc cacatcaaac gccgattacg acggcgagct
    12301 ccgcggcggc tcgttcttcg atgccgccag gttcccgcgc gcgacgtaca tcgcgacgac
    12361 gttccgcgcg ctcggcggca accgttacgc agccgatggc accttgaccc tgcgcggcat
    12421 cagcaagccg gtggttctgg aattcacctg gacgcccggc gcggcgcccg tgctgtcggg
    12481 caaggccacg gtgaaacggc tggcgttcgg cgtgggtggc ggcgactggg cggataccgc
    12541 gctgatcccg gacgccatcg cgatcagcac caaggtcgtg ttcaaggcca aatagccgcg
    12601 cgccatgtgc gacgcgcgtg gccatggcac cctgcccgac cacgcacaca taccctgcgg
    12661 agcccgcatg cccctgttcc ccctgttcac cgacctgcag ggccgcgagg tgctggtcgt
    12721 cggcggtggc gaggtcgcga cccgcaagat ccacgcgttg ctgcgggcgg gcgcacgggt
    12781 gctgatctat gcccgtgaac tggcaccgcc actgcaggcg ttggccgtgg cgggacacct
    12841 gcaacgactg gagggcgatg cggtcgagtc cgcctggatc gaacgcgcct ggctggtggt
    12901 ggcggcgacg gacgaccacg tgctcaaccg cgaagtcgcc ggtatggccg aagctgcgaa
    12961 gcgctggatc aacgtggtcg acgatgccga gctgtcgagc taccaggtcc cggcggtggt
    13021 cgaccgcgag ccgatcacca tcgcgatttc ctccgcgggc gcggctccga tgctggcgcg
    13081 ccagttgcgc gagcagctcg aggccgagat cgatccgtcg atcggcgagc tcgcccgcct
    13141 gttcgcacgg catcgtggcc gcatccgcga gcgactgccg ctgatgcgcg aacgccgcga
    13201 ctggttcgag cggatcctcg ccggcgacat cccggtgcta ctgcgcgctg gcaacgctga
    13261 cgcagcggag caggccttca aggacgccct ggacgaaggc ggcacgtcgc gggtgcgcgg
    13321 ctcggtggcc ctggtcggct gcggcgacgg cgatcccggc ctgctcgcgc tgcgcgcgct
    13381 gcgcctgctc aaccaggccg acctggtcct ggtcggcgac ggcgtggccc aggcgatcgt
    13441 cgacatggcc aggcgcgacg cggcgatgca gccattgccg tccgccgaag atctgtccga
    13501 ggtactgtcg gcacatgtcg aggccggcag gcgcgtcgtc tgcctgcgtc ccggcagcgg
    13561 ctttaccgat gccgccggac gcgtcctgcg caccacgctc cacgaacgcg ggcacgcctg
    13621 cgaaaccttg ccgggcgcgg cctggcccag cgactgagcg aacgcccgcg ccgccgagct
    13681 attccatgcc aaggaaagcc cgcaccgcat ccacgtcgaa tggccagtcc agctcagtgc
    13741 caccgacatg ctcgcgaagc accggcactc gcaacccgta tcgcgcttcc agcgcggcgt
    13801 catcgtcgat gaagacactc tcgaaatccg gcgcgcgcgc ctcggccagc accgcgagcg
    13861 cgaggtcgca gagatggcag gtatcgcgct ggtagaggat cagttccata cgcctgcgtc
    13921 agtggccgtc tcaccgggcg gcctagaata gcggcccgtt cccgccacgc agtgcgccgc
    13981 catggccgtc tccaccttcg acctgtacaa gatcggcatc ggcccgagct cctcgcacac
    14041 ggtcgggccg atgcgcgcgg cggcgcgttt cgtcgaacat cacctggccg aagccggccg
    14101 cctggacgat gtcgcccggg tgcgcgccga ggtgttcggc tcgctcgccc tgaccggccg
    14161 cggccacggc accgacaagg cggtgctgat gggcctggaa ggccactggc cgaaccggat
    14221 cgatcccgac gtcatcccgg ccgcgctgga acgcatccgc ggcagcaagt cgatccggct
    14281 gcatgggcag cgcgagatcg ccttcaacga gaagtcggac ctggtgatga acaagcggca
    14341 gaaactgccg ttccacacca atggcatgcg tttcatcgcc tacgacgcca acggcgctga
    14401 ggtcgccagc cgcgattact actcggtggg tggcggcttc gtggtcaacc aggacgaggc
    14461 cgcggaagac cgcatcgtcg ccgacaccac gccggtcgcg catccgttcg ccagtggcga
    14521 cgagttgctc gcgcgttgcg cggcctcggg cctgtcgatc gcgcagctga tgctggagaa
    14581 cgaacgcgcc tggcgcagcg acgacgaggt gatcgccggg ctgcgcgagc tgtggaccgc
    14641 catgcaggac tgcgtggccc gcggtatccg cgagaccggc accctgcccg gcggcctgca
    14701 tgtctcgcgg cgcgcgccgt cgttgcacgc ggaattgtcc ggcaagccgg aggccgcgat
    14761 gcgcgacccg ctgaccgtgc tggactgggt caacctgtac gcgctggcgg tgaacgagga
    14821 gaacgccgcc ggtggccgcg tggtcaccgc gcccaccaat ggcgcggccg ggatcatccc
    14881 ggcggtgctg cactactacg accgcttctg cgccggcgcc agcgagaagg gcgtgttcga
    14941 gttcctgctc accgcctctg cggtcggcat cctctacaag gagaacgcca gcatcagcgg
    15001 cgccgaggtc ggctgccaag gcgaggtcgg cgtggcctgc tcgatggccg ccgccggcct
    15061 gaccgcggcg ctcg
//
LOCUS       DAOOCX010000055        15042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3590_length_15042_cov_8.751385, whole genome shotgun sequence.
ACCESSION   DAOOCX010000055 DAOOCX010000000
VERSION     DAOOCX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15042
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3590_length_15042_cov_8.751385"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1322)
                     /locus_tag="PK399_06810"
     CDS             complement(78..1322)
                     /locus_tag="PK399_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPW12863.1"
                     /translation="MPAGTLLYAQSGGVTAVINATASAVLQAARARKVRVLAARNGIL
                     GALREELIDTTTLSTAQVKALAHTPGGAFGSCRLKLKSVEADRARYERLLAVLQAHDV
                     RWFLYNGGNDSADTALKVSQLAAEFGYPLTCVGVPKTIDNDLAVTDCCPGFGSAAKYT
                     AVSVRECALDVAAMAETSTKVFVYEAMGRHAGWLAAAAGLAGEGRDEAPHLILFPERP
                     FDEADFLARVDAVVKRVGHCVVVASEGIRTADGTFVADAGGGKDAFGHAQLGGVASHL
                     AGRVKDVLGHKVHWALPDYLQRSARHLASKTDLEQAMAVGRAAVDCALAGQNAVMPVI
                     VRTADEPYEWHVDVAPLSKVANHEKTMPKGFIRADGHGITAAARRYLTPLIAGEAYPP
                     YGRNGLPDYVVLPQATIRRAPR"
     gene            1452..2030
                     /locus_tag="PK399_06815"
     CDS             1452..2030
                     /locus_tag="PK399_06815"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPW12864.1"
                     /translation="MRLVLLGAPGSGKGTQAARLKEHLQVPHISTGDLLRAEVAAGTP
                     LGLQAKEVMARGDLVSDAILLGMLKDRFSRDDTKAGFILDGYPRNLAQAAALDDLLAS
                     LGQKFDAAVQLTVDNEQIIERLAGRAKAEGRADDSPDSVRHRLNVYDQQTAPVIDFYR
                     QHGQLTVVDGVGSLDEVFTRIVEAIQPGHEVG"
     gene            2178..3539
                     /gene="mpl"
                     /locus_tag="PK399_06820"
     CDS             2178..3539
                     /gene="mpl"
                     /locus_tag="PK399_06820"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HPW12865.1"
                     /translation="MKLHILGIAGTFMGGVAALARELGHAVEGSDQNIYPPMSTQLEQ
                     LGIALKQGYSPANISGDCDEIVVGNALSRGNEAVEHVLDAGLRYTSGAQWLSEQVLPG
                     RDVLAVAGTHGKTTTTTILSYLLEAAGRSPGFLIGGVAEDFGVSARIGSGPDFVVEAD
                     EYDTAFFDKRSKFVHYRPLVAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPRRGRLV
                     VNGHDERLREVLAMGCWTPVERFGLDASFEWSARKLRDDGSMFAVMRAGEQLGIVEWP
                     LLGDHNVLNGLAALAACAAVGVDAAAIIPSLAAFSSVKRRMEVIGERAGITLYDDFAH
                     HPTAIATTLAGLRAKVGPARIVVAMEPRSNSMRLGAHAEALAPSLDIADVVVFLARPE
                     LPWDAGKVVGALKGEGHVVAGADALIARLRTVVRAGDHVVFMSNGGFDGAPRRFHAAL
                     QDG"
     gene            3532..4128
                     /locus_tag="PK399_06825"
     CDS             3532..4128
                     /locus_tag="PK399_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPW12866.1"
                     /translation="MADAAASLPLFPLHGVLVPGAALDLRIFERRYLDMVGECGRNGS
                     GFGVCLILEGNEAGAPATPVAFGTEAVIEDFGNTPEGLLSLRVRGRRRFHVLRTRVRD
                     TGLVVADIEWRADAGASSRLRPEHALLAELLRRILLQVDAGHVDPDPALFDDAAWTGW
                     RLAELLPLSMPQRQALLQLDDPHARLQFLLDGIAADPN"
     gene            complement(4138..6069)
                     /locus_tag="PK399_06830"
     CDS             complement(4138..6069)
                     /locus_tag="PK399_06830"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DedA family/phosphatase PAP2 family
                     protein"
                     /protein_id="HPW12867.1"
                     /translation="MQSAWFDATIAWITAHPHAAGGLVFLIAFCDALAVVGIIVPALP
                     LLFAVGTLIGLGHIDGPYAVTCAALGAFIGDGLSYWIGHRWGPAMRELWLFRRYPQLL
                     DRGETLFRRHGTKGLVIARFVGAVRPFVPAVAGMLHMPLRRYMPASLFACFAWAALFL
                     APGWIFGASYDAVAAVADKLVLVLLGLVAVLALVWALVLYTWRWFANHADTLLARGLR
                     WTRAHPRLGRYAAALIDPRRPESASLLVLACCLFVVAWLWAGFSASLLMRGGPSLFDH
                     DVHAAMFALRNPLADRLMASLSAIGSAPVLGFPALVAMGWLLWRKRWMAAAHWLGAIA
                     VGLALTVGLGAVADMPRPPTAVAGFGFPSVAVTMTTVVFGFFAVLIARELPGRQRVWP
                     YLLAGVATALVGFARLYLGAHWLSDITGGVLLGVIWLLVLGIAYRRHSARSFWMRPLA
                     WAFYGSFLLVALWHAPRSAEATLAQFEPPAPTHVIDDTRWWSQGIDDPRHFDLEAAGN
                     LGVLQRRLQAQGWTVQAPADWVAVLGLLDDDHALAEQPVLPLALDGHPERLLLRRAGP
                     LADELEVLRVWPAPARLASGKPLWIARYERMHGRKRLRLLTLWQPEPHAHALPDDVLQ
                     LAGTSAERDATLRIRIDPP"
     gene            6141..6959
                     /locus_tag="PK399_06835"
     CDS             6141..6959
                     /locus_tag="PK399_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005419027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW12868.1"
                     /translation="MASLSGKTLFITGASRGIGLAIALRAARDGANVAIAAKSDVANP
                     KLPGTIHSAAQAVNDAGGRGLALKCDIREEDQVRASMAATVDAFGGIDILVNNASAIW
                     LRGTLDTPMKRFDLMQQVNSRGSFLCAQACLPYLLEAPNPHILTLCPPPSLDPKWWAP
                     HTGYTLAKMGMSFVTLGLAAEFGPRGVAVNALWPRTLIATDALNMIPGVQAGNGRSPA
                     IMGDAAHAVLVRDARTTSGRFFIDDEVLAETGVTDLSGYAIDPSLPLLPDLFLD"
     gene            7158..7589
                     /locus_tag="PK399_06840"
     CDS             7158..7589
                     /locus_tag="PK399_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW12869.1"
                     /translation="MKYLHTMVRVRDLDASLRFYCDGLGLKETRRMENEQGRYTLVFL
                     AADETPNAEVELTYNWPGENGATEDYGSARNFGHLAFRVADIYATCERLQAMGVTINR
                     PPRDGHMAFVRSPDLVSIELLQDGHLEPREPWASMPNTGVW"
     gene            7601..7990
                     /locus_tag="PK399_06845"
     CDS             7601..7990
                     /locus_tag="PK399_06845"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HPW12870.1"
                     /translation="MQGLTDFVAYFGMGLGFMLLYLLLYLYATPHREITLIRGDNLPA
                     AVVLAGALLGFALPLASALRIGGGVIDVAAWASIALFAQLAAYALVALLLRDFSKRIN
                     RGEMAAAVMAATIHLGVGLLNSAAMSF"
     gene            8004..9239
                     /locus_tag="PK399_06850"
     CDS             8004..9239
                     /locus_tag="PK399_06850"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HPW12871.1"
                     /translation="MTDAVTDTAQLLALGRDFYLPVYKPRQLILERGRGSRVWDREGR
                     EFVDLAGGIAVCGLGHCDPDLTAALVEQAGKLWHTSNVFYSEPPVRLAEELVSASRFA
                     ERAFLCNSGAEANEAAIKLVRKWATGQGRAADRRVIVTFRGSFHGRTLAAVTATAQPK
                     YQEGYEPLPGGFRYVDFNDIAQLDAAMAGGDVAAVMLEPVQGEGGVMPAAPGFLQAVR
                     ALCDRHDALLVLDEIQCGMGRSGALFAHWQDHVVPDIVTLAKALGGGMPIGALLAGPK
                     VAQVMQFGAHGTTFGGNPLASAVARVALRKLGSDGIAANVGRQAQAIRDGLDAINRDL
                     DLFAEVRGRGLMLGAVLKPACAGRAGEILDHAAANGLLLLQAGPDVLRFVPALNIGDE
                     DVAEGLSRLRAALQAFSAG"
     gene            complement(9257..10186)
                     /locus_tag="PK399_06855"
     CDS             complement(9257..10186)
                     /locus_tag="PK399_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016850092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW12872.1"
                     /translation="MRVDRRGFLATLAASGLLAAAPRVVASGPESRLVVLGTAGGPTP
                     KPNRFPAAYALVVDEDVYLVDAGNGVAQQLARAGIGINRVRHVFISHHHSDHDADAGA
                     VALLAWATNPTSPVTIWGPPPMRAQMAAYREYARFDIDTRTADEGRPDFDAMTRVVEF
                     NQEGLLLDDGTIKVRCVRNLHPPIRESYALRFDTPGRSYVFSGDTTYSPAVVALAKGA
                     DVLVHEVMYLPALDALIASEPNASTLREHLLASHSTPEQVGRVATEAGVGTLVLSHFV
                     PGGPMVADETWLAAVRPHFGGRIVVAHDLMTLP"
     gene            complement(10193..11320)
                     /locus_tag="PK399_06860"
     CDS             complement(10193..11320)
                     /locus_tag="PK399_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPW12873.1"
                     /translation="MTTLRPDTLAVHAGNAPDDATGAVAPPLHLSTTFRHGPAGERVA
                     GYEYQREGNPTNDRLREALAALEGGETACTFASGMAAMTTLLECLPNGARVLFPDDCY
                     SGLRMLFQEFLPERGLVAEVIDMGDLDAVRAACATPVSMLWIETPSNPLMKVSDIAAL
                     ADIGHAAGAVIVVDNTFATPLLQRPLALGADVVMHSTTKYFGGHSDVLGGALVFAHDG
                     ELARKVAHRLHVTGAVLAPFSAWLTLRGCRSLGARMAMHCANARKVVDFLAAHTAIER
                     VNWPGLSSHPGHEVANRQMRDFGAMLSIEVRGGREAALAMAGKLRVFTNATSLGGCES
                     LVEHRASVEGPNPRSPQNLLRISVGLEDADDLVADLAQALG"
     gene            complement(11328..12212)
                     /locus_tag="PK399_06865"
     CDS             complement(11328..12212)
                     /locus_tag="PK399_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HPW12874.1"
                     /translation="MQTDDRQRDAPRSLFEREHWPATTEGARYRWYRLIFHHDSPAER
                     NFDLWLIVAILASVAVVMLDSVPAVKQRWHVPLSLAEWFFTLVFAIEYLVRLWTVRRP
                     LRYATSFFGIIDLLAILPTLLSLLFPAGTSLAVVRILRLLRIFRVLKLAEYSSEAGVL
                     VQALLRSRRKILVFIATLLTITVIFGAMMYVIEGPAHGYTSIPTGMYWAVVTMATVGF
                     GDIAPATTLGRLVTSVLIIIGYSIIAVPTGIYTAELARSMRPSRNNVRCTECGLIDHE
                     ADAWHCRKCGRALPCHDP"
     gene            complement(12214..13497)
                     /gene="hemL"
                     /locus_tag="PK399_06870"
     CDS             complement(12214..13497)
                     /gene="hemL"
                     /locus_tag="PK399_06870"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPW12875.1"
                     /translation="MNNARSHDLFTRAQALMPGGVNSPVRAFKSVGGEPFFVQRADGP
                     HLFDADGNRYIDYVGSWGPMIVGHNHPKVLDAVIATARNGLSFGTPNPLEVTMAETIT
                     RLIPSCEMVRMVNSGTEATLSAIRLARGATGRSRIVKFEGCYHGHGDSFLVKAGSGAL
                     TFGVPTSPGVPKALADLTLTLTYNDFDGATRLFEECGSEIACLIVEPVVGNANCLPPR
                     EGYLQHLRDLCTRHGALLVFDEVMTGFRVALGGAQARYGVTPDLSTFGKVIGGGMPVG
                     AYGGRRELMQQIAPAGPIYQAGTLSGNPVAMAAGLAMLELVQAPGFHDALEAKTNALC
                     DGLEAAARDAGIAFTTQRVGGMFGMFFSNDIVDTYAQAMACDSAAFNRFFHAMLERGV
                     YLAPSAFEAGFMSSAHDEAAIADTIRAAGEAFRSL"
     gene            complement(13494..14141)
                     /gene="thiE"
                     /locus_tag="PK399_06875"
     CDS             complement(13494..14141)
                     /gene="thiE"
                     /locus_tag="PK399_06875"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPW12876.1"
                     /translation="MPSNPLKRGLYLITPDDPDPARLLARTTPLLAFASCLQLRNKAM
                     DVDSLRDAGRRLRHACNHAGVPLIVNDDPALARELDADGVHLGEADGDIASARTLLGD
                     RAIIGVSCYDDIDRARRLAAEGAGYLAFGAFFASPTKPHARRASLDLLRDSADLGLPR
                     VAIGGITADNAPSLVAAGADLVAVISGVFDAADPVAAARAYHSCFDTGIPGTQSE"
     gene            14204..14395
                     /locus_tag="PK399_06880"
     CDS             14204..14395
                     /locus_tag="PK399_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPW12877.1"
                     /translation="MTASESDVPYRQWMCVVCGFIYDEALGLPEDGIAPGTRWEDVPD
                     TWTCPDCGVSKDDFEPVEA"
     gene            complement(14402..14848)
                     /locus_tag="PK399_06885"
     CDS             complement(14402..14848)
                     /locus_tag="PK399_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPW12878.1"
                     /translation="MRLYALVLLVSCGISGCAGASQPWVEIGGQRYVVEVSDDDAERA
                     RGLMFRDTMASDHGMLFIHDAEEPQAYWMKNTKIPLDILYFDSERKLVAQQRDVPTCS
                     LGDACPSYPSDAPARYVLELNAGEAARLGLKDGAEMRFSPAIKPAK"
BASE COUNT         2446 a         5083 c         5184 g         2329 t
ORIGIN      
        1 gccctcggtg cccagcagcg tggccggggt gttggcgaac tcgagcttgg cctgcttgcg
       61 ggtctggtcc atggtgctca gcgcggtgcg cgccgtatcg tggcttgcgg gagtactacg
      121 taatcgggca gtccgttccg gccataaggg gggtaggcct cgcccgcgat cagcggggtc
      181 agatagcggc gcgcagcggc ggtgatgcca tggccgtcgg cgcggatgaa ccccttcggc
      241 atggttttct cgtgattggc gaccttcgac aacggcgcaa catcgacgtg ccactcatac
      301 ggttcgtcgg cggtgcgcac gatcaccggc atcaccgcgt tctgcccggc caatgcgcag
      361 tccaccgcgg cgcgaccgac cgccatcgcc tgttcgaggt cggtcttcga ggccaggtgg
      421 cgggcggagc gttgcaggta gtcgggcagc gcccagtgca ccttgtgacc gagcacgtcc
      481 ttgacccgcc cggccaggtg cgaggccacg ccgcccagtt gcgcatgccc gaacgcatcc
      541 ttgccgccgc cggcatcggc cacgaatgtc ccgtccgcgg tgcggatgcc ctcgctggcc
      601 acgaccacgc aatggcccac ccgcttcacc acggcatcca cgcgtgccag gaaatcggct
      661 tcgtcgaacg gccgttccgg gaacaggatc aagtgcgggg cctcgtcgcg gccttcgccg
      721 gccagtcccg cggccgcggc cagccaaccg gcatgccggc ccatcgcttc gtacacgaag
      781 accttggtcg aggtctcggc catcgccgcc acgtccagcg cgcattcgcg caccgacacc
      841 gcggtgtact tcgccgccga gccgaagccg gggcagcagt cggtgaccgc caggtcgttg
      901 tcgatcgtct tcggcacgcc gacgcaggtc agcggatagc cgaactcggc tgccagctgc
      961 gagaccttca atgcggtgtc ggcggaatcg ttgccgccgt tgtagaggaa ccagcgcacg
     1021 tcatgcgcct gcaggacggc gagcaggcgc tcgtacctgg cgcggtcggc ctcgaccgat
     1081 ttcagcttga gcctgcagct gccgaacgcg ccaccgggcg tatgcgccag cgccttgacc
     1141 tgggcggtcg agagggtggt cgtatcgatg agttcctcgc gcagcgcgcc gaggatgccg
     1201 ttgcgggcgg ccagcacccg cactttccgg gcccgggcgg cctgcagcac ggcggaggcg
     1261 gtggcgttga tgacggcggt gacgccgccg ctctgggcgt acagcaaggt gcctgcgggc
     1321 atgccggttt cctcggggga taggcccttg gtcgaagccg cgggccggac gcataacgat
     1381 acggtaagct ggcccggacg aggccggcac gcgtggcgtg gcggccgccc cccgatttct
     1441 ggagcgatgc gatgcgattg gttctgttgg gcgcgccggg ttccggcaag ggcacccagg
     1501 cggcacgact gaaggaacac ctgcaagtcc cgcacatctc caccggcgac ctgttgcgcg
     1561 ccgaagtggc cgccggcacc ccgttgggcc tgcaggccaa ggaagtgatg gcgcgaggcg
     1621 acctggtcag cgacgcgatc ctgctgggca tgctgaaaga ccgtttctcg cgcgacgaca
     1681 ccaaagccgg cttcatcctc gatggctatc cgcgcaacct ggcgcaggcg gcggccctgg
     1741 acgacttgct tgccagcctc gggcagaaat tcgatgccgc cgtccagctc accgtggaca
     1801 acgagcagat catcgagcgc ctggccggcc gtgcgaaggc cgaaggccgc gccgacgaca
     1861 gcccggactc ggtccgccac cgcctcaacg tgtacgacca gcagactgcg ccggtgatcg
     1921 acttctaccg ccagcacggc cagctcaccg tggtcgatgg cgtgggctcg ctggacgagg
     1981 tattcacccg catcgtcgag gcgatccagc cggggcacga ggtcggttga cgtagttcct
     2041 tctcccgcat gcgggagaag gtggcccgca gggccggatg agggtgtgct ctcgttccac
     2101 cctcacccca accctctccc gccagcggga gagggagaaa agcagaaatg cgatcagccc
     2161 ggaatgacaa gcacagcatg aaactccaca tcctcggcat cgccggtacc ttcatgggtg
     2221 gcgtcgccgc gctggcgcgc gaactcggcc atgcggtcga gggcagcgac cagaacatct
     2281 atccgccgat gtcgacccaa ctggaacagc tgggcatcgc gctgaagcaa ggctattcgc
     2341 ctgcgaatat ttcaggcgac tgcgacgaga tcgtggtcgg caatgcgctg tcgcgcggca
     2401 atgaggcggt cgagcacgtg ctcgatgccg gcttgcgcta cacgtcaggc gcgcagtggt
     2461 tgagcgaaca agtcctcccg ggtcgcgacg tgctggcggt cgccggcacc cacggcaaga
     2521 ccacgacgac gaccatcctg tcctacctgc tggaagcagc cggtcgttcg ccgggcttcc
     2581 tgatcggcgg cgtggccgag gatttcggcg tgtcggcgcg catcggcagc ggacccgact
     2641 tcgtggtcga ggccgacgaa tacgacaccg ccttcttcga caagcgcagc aagttcgtcc
     2701 actaccgccc gctggtcgcg atcctcaaca acctcgagta cgaccacgcc gacatcttcc
     2761 cggacgtggc ggcgatccag cgccagttcc atcacctggt gcgcaccgtg ccgcgccgcg
     2821 gccggctggt cgtcaacggc cacgacgaac gcttgcgcga agtgctggcg atgggctgct
     2881 ggacgccggt ggagcgcttt ggtctggatg cgtcgttcga gtggagcgca cgcaagttgc
     2941 gcgatgacgg cagcatgttc gcggtgatgc gtgctggtga acaactcggc atcgtcgaat
     3001 ggccgctgct cggcgaccac aatgtgctca acggcctggc cgcgctggcg gcctgcgcag
     3061 cggtcggcgt ggatgccgcg gcgatcatcc cgtcgctggc cgccttcagc agcgtcaagc
     3121 ggcgcatgga agtgatcggc gaacgcgccg gcatcaccct ctacgacgac ttcgcccacc
     3181 atccgaccgc gatcgcgacc acgctggcgg gtttgcgcgc gaaggtcggc cccgcccgca
     3241 tcgtggtggc gatggaaccg cggagcaatt cgatgcggct gggagcgcat gccgaggcgc
     3301 tcgccccgtc gctcgacatc gccgatgtgg tggtgttcct ggcccggccg gaattgccgt
     3361 gggatgcggg caaggtcgtc ggtgcactga aaggcgaggg acacgtggtg gccggtgccg
     3421 atgcactgat cgcgcggttg cgtacggtcg ttcgcgctgg cgaccacgtg gtgttcatgt
     3481 ccaacggcgg cttcgatggc gcgccgcggc ggttccatgc cgccctgcag gatggctgac
     3541 gccgccgcgt cgctgccgtt gttcccgttg cacggcgtgc tggtgccggg tgccgcgctg
     3601 gacctgcgga tcttcgagcg tcgctacctc gacatggtcg gcgagtgcgg gcgcaacgga
     3661 tccggcttcg gggtctgcct gatcctggag gggaacgaag ccggcgcgcc ggcgacgccc
     3721 gtcgccttcg gcaccgaagc cgtgatcgag gatttcggca acacgccgga aggcttgttg
     3781 tcgctgcgcg tgcgcggccg ccgccgtttc cacgtcctgc gcacccgcgt gcgcgacacc
     3841 ggcctggtcg tcgccgacat cgagtggcgc gcggatgcgg gggcatcgtc gcgcctgcgc
     3901 ccggaacatg ccttgttggc cgaattgctg cgacggatcc tgctgcaggt cgacgccggc
     3961 cacgtcgatc cggatcccgc gttgttcgac gacgccgcat ggacggggtg gcggctggcc
     4021 gaactgttgc ccttgtcgat gccgcagcgg caggcgttgc tgcaactcga tgacccgcac
     4081 gcgcgcctgc agttcctgct cgacggcatc gccgccgacc ccaactgatc gctgcgctca
     4141 gggcggatcg atgcggatcc gcagcgtcgc gtcgcgctcc gcggaggtcc cggcgagctg
     4201 cagcacgtca tcgggcaatg catgcgcatg cggttccggc tgccacaggg tcagcaggcg
     4261 caagcgcttg cgtccgtgca tgcgttcgta gcgggcaatc cagagtggct tgccgctcgc
     4321 cagtcgcgcc ggcgcgggcc agacgcgcag cacttcaagc tcgtcggcca gggggccggc
     4381 gcggcgcagc agcaatcgct ccgggtgtcc gtccagggca agcggcagta ccggctgctc
     4441 ggcgagcgca tggtcgtcgt ccagcaggcc gagcaccgcg acccagtcgg ccggggcttg
     4501 cacggtccag ccctgtgcct gcaatcgccg ctgcagcacg cccaggttgc cggcggcttc
     4561 aagatcgaag tgacgcggat cgtcgatgcc ctgcgaccac cagcgcgtgt cgtcgatgac
     4621 gtgcgtcggt gccggcggct cgaactgcgc aagcgtcgct tccgcgctgc gcggtgcatg
     4681 ccagagcgcg accagcaaga agctgccgta gaacgcccac gcgagcggcc gcatccagaa
     4741 cgagcgcgcg ctgtggcggc gataggcgat gccgagcacg agcagccaga tcacccccag
     4801 cagaacgccg ccggtgatgt cgctcagcca gtgcgcgccc aggtacagcc gcgcgaagcc
     4861 gaccagcgcc gttgccacgc cggccagcag gtaaggccac acacgctggc ggccgggcag
     4921 ttcgcgcgcg atcagcaccg cgaagaagcc gaacaccacg gtcgtcatcg tcaccgcgac
     4981 cgaggggaag ccgaaccccg cgacggcggt cggcgggcgc ggcatgtcgg cgaccgcgcc
     5041 caggccgacg gtcagcgcca agcccacggc gatcgcaccc agccagtggg ctgcggccat
     5101 ccagcgcttg cgccacagca gccatcccat cgccaccagc gcggggaacc ccagcaccgg
     5161 tgcgctgccg atcgcggaca gcgatgccat caggcggtcg gccagcggat tgcgcagggc
     5221 gaacatcgcc gcatgcacgt cgtggtcgaa cagcgacggc ccgccgcgca tgagcagcga
     5281 ggcactgaag ccagcccaca gccaggcgac cacgaacagg cagcaggcca gcaccagcag
     5341 ggacgcggat tccggccggc gcggatcgat cagcgcggcg gcgtagcggc ccagccgcgg
     5401 atgcgcacgc gtccagcgca ggccgcgggc gaggagcgtg tcggcgtggt tggcgaacca
     5461 gcgccatgtg tagagcacca gtgcccagac cagcgccagc actgcgacca ggcccagcag
     5521 caccagcacc agcttgtccg ccaccgccgc caccgcatcg taggacgcac cgaagatcca
     5581 acccggtgcc aggaacagcg cggcccatgc gaagcaggcg aagaggctgg ccggcatgta
     5641 acgccgcaac ggcatgtgca gcatcccggc caccgccggc acgaacgggc gcaccgcgcc
     5701 cacgaatcgc gcgatcacca ggcctttcgt cccgtgccgg cggaacaggg tctcgccgcg
     5761 atccagcagt tgcgggtagc ggcggaacag ccagagttcg cgcatcgccg gtccccagcg
     5821 atggccgatc cagtaactca ggccatcgcc gatgaaggcg cccagcgcgg cgcaggtcac
     5881 tgcgtacggg ccgtcgatgt gtcccaggcc gatcagggtg ccgaccgcga acagcaacgg
     5941 cagcgcggga acgatgatac cgaccaccgc cagtgcatcg cagaaggcga tcaggaatac
     6001 gagcccgccc gcggcatgcg gatgagcggt gatccaggcg atggtggcgt cgaaccaggc
     6061 gctctgcatc ggccgattat agaaggtggg atgcaggcat cggattacac tcccggctca
     6121 ccccactgat ggcgcgcgtg atggcctctc tttccggcaa gaccctgttc atcaccggcg
     6181 cctcgcgcgg catcggcctg gccatcgcac tgcgcgccgc gcgcgatggt gcgaatgtcg
     6241 ccatcgcggc caagtcggat gtcgccaatc ccaagctgcc gggcaccatc catagcgcgg
     6301 cgcaggccgt gaacgatgcc ggtgggcgtg gccttgcgct gaaatgcgac atccgcgagg
     6361 aagaccaggt gcgcgcgtcg atggccgcca cggtcgatgc gttcggcggc atcgacatcc
     6421 tggtcaacaa cgccagcgcg atctggctgc gcggcacgct ggacacgccg atgaagcgct
     6481 tcgacctgat gcagcaggtc aattcccgcg gcagtttcct gtgtgcgcag gcttgcctac
     6541 cgtacctgct ggaggcgccg aatccgcaca tcctgaccct gtgcccgccg ccttcgctgg
     6601 atccgaaatg gtgggcaccg cacaccggct acaccctggc gaagatgggc atgagcttcg
     6661 tcaccctggg cctggccgcg gagttcggcc cgcgtggcgt cgcggtgaat gcgttgtggc
     6721 cgcgcacgct gatcgccacc gatgcgctga acatgatccc cggtgtgcag gctggcaacg
     6781 gccgttcgcc ggcgatcatg ggcgatgccg cgcatgcggt gctggtgcgc gacgcacgca
     6841 ccaccagcgg ccgcttcttc atcgacgacg aggtgctggc cgaaaccggc gtcaccgacc
     6901 tgtccggcta cgcgatcgat ccgtcgctgc cgctgctgcc cgacctgttc ctcgactgag
     6961 tcgtcagcac ttgccgccgt cgtgtcgtcc tccggggacg tgcgatggcg ggtgcgacgt
     7021 gaaccaggcc gatggcgtgc cggcagcagc agcgctacct gccggcacgc aacatcggcc
     7081 gcgcggcgca ccgggcgatg ctgcagggcc gggcatcgcg tgggatcatg tcgcctcccc
     7141 cacgcgatgc caccgccatg aaatacctgc acaccatggt ccgcgtgcgc gacctcgacg
     7201 cctccctgcg cttctactgc gacggcctgg gcttgaagga aacgcgccgc atggagaacg
     7261 aacagggccg ctacaccctg gtgttcctcg ccgccgacga gaccccgaat gccgaagtcg
     7321 agctgaccta caactggccc ggcgaaaacg gtgcgaccga ggattacggc agcgcccgca
     7381 acttcggcca cctggccttc cgagtggccg acatctacgc cacctgcgaa cgcctgcagg
     7441 cgatgggcgt caccatcaac cgcccgccgc gggatggcca catggccttc gtgcgttcgc
     7501 ccgacctggt ctccatcgag ctgctgcagg atggccatct cgaaccgcgc gagccgtggg
     7561 cgtcgatgcc gaacacgggc gtatggtgag cgtacgcgac atgcaggggt tgaccgattt
     7621 cgtcgcctac ttcggcatgg gcctggggtt catgctgctg tacctgctgt tgtacctgta
     7681 cgccacgccg caccgcgaaa tcaccctgat ccgtggcgac aacctgcccg ctgcagtggt
     7741 gctggcgggt gcgttgctcg gcttcgcctt gccgctcgcc agtgccttgc gcatcggtgg
     7801 cggcgtgatc gacgtggcgg cgtgggcatc gatcgcgttg ttcgcacaac tggcggcgta
     7861 tgcgctggtc gccctgctgc tgcgcgattt ctccaagcgc atcaaccgcg gcgaaatggc
     7921 ggcggcagtg atggccgcga cgatccacct gggcgtcggc ctgctgaatt ccgccgcgat
     7981 gagtttctga cgagcctccc acgatgaccg atgccgtgac cgataccgcg cagctgctgg
     8041 ccctgggccg tgatttctac ctgccggtgt acaagccgcg ccaattgatc ctggagcgtg
     8101 gccgcggctc ccgcgtctgg gatcgcgagg gacgtgaatt cgtcgacctg gccggcggca
     8161 tcgccgtgtg cgggctcggc cattgcgatc cggacctgac cgccgcactg gtcgaacagg
     8221 ccggcaagct ctggcatacc agcaacgtgt tctacagcga gccgccggtg cgactggcgg
     8281 aagagctggt ttcggcgtcg cgcttcgccg agcgcgcctt cctgtgcaac tccggcgccg
     8341 aggccaacga ggccgcgatc aagctggtgc gcaagtgggc gacggggcag ggccgcgcgg
     8401 cggaccgccg cgtcatcgtc accttccgcg gcagtttcca tggccgtacc ctggccgcgg
     8461 ttaccgcgac cgcgcagccg aaataccagg aaggctacga gccgctgccc ggcgggttcc
     8521 gctacgtcga cttcaacgac atcgcccagc tcgacgcggc gatggccggc ggcgacgtcg
     8581 ccgcggtaat gctggagccg gtgcagggcg agggtggggt gatgccggcg gcgccgggct
     8641 tcctgcaggc ggtgcgcgcg ctctgcgacc gccacgacgc gctgctggtg ctcgacgaga
     8701 tccagtgcgg catgggtcgc agcggcgcgt tgttcgcgca ctggcaggac catgtggtgc
     8761 cggacatcgt gacgctggcg aaggcgctgg gcggcggcat gccgatcggt gcgctgctgg
     8821 ccggcccgaa ggtcgcgcag gtcatgcagt tcggcgcgca tggcaccacg ttcggcggca
     8881 acccgctggc gtcggcggtc gcccgcgtcg cgctgcgcaa gctgggctcg gacggcatcg
     8941 ccgccaacgt agggcgccag gcgcaggcca ttcgcgatgg cctcgatgcc atcaatcgcg
     9001 acctcgacct gtttgccgag gtgcgtggac gcggcctgat gctgggtgcg gtgctgaagc
     9061 ccgcctgcgc ggggcgcgcg ggcgagatcc tcgatcacgc agcggccaat ggcctgctgc
     9121 tgttgcaggc cgggccggac gtgctgcgct tcgtgcccgc attgaacatc ggcgacgagg
     9181 acgtggccga agggctgtcg cgcttgcggg cggcattgca ggcgttcagc gccggctgag
     9241 cgaggttggc gttgcctcag ggcaacgtca tcaggtcatg ggcaacgacg atgcggccgc
     9301 cgaaatgggg ccgcactgca gccagccagg tttcgtccgc gaccatcggc ccgccgggca
     9361 cgaaatgcga cagcaccagc gtgcccacgc cggcctcggt agcgacgcgt ccgacctgtt
     9421 ccggcgtgga atggctggcg agcaggtgct cgcgcagggt gctggcgttc ggctcactgg
     9481 cgatcagcgc gtccagcgcg ggcaggtaca tcacttcgtg taccagtacg tccgcgccct
     9541 tggccagggc gacgaccgcg ggcgaatacg tggtgtcgcc ggagaacaca tagctccgac
     9601 cgggcgtgtc gaagcgcagc gcatacgatt cgcggatcgg cggatgcagg ttgcgcacgc
     9661 agcgcacctt gatcgtgccg tcgtccagca acagcccttc ctggttgaac tccacgacgc
     9721 gggtcatcgc atcgaagtcg ggccggcctt catcagcagt acgcgtgtcg atgtcgaagc
     9781 gggcgtactc gcgataggcc gccatctgcg cgcgcatcgg cggcggtccc cagatcgtca
     9841 ccggcgacgt cgggttggtc gcccacgcga gcagcgccac cgcgccggca tccgcatcgt
     9901 ggtcggaatg gtggtggctg atgaacacgt gtcgcacccg gttgatgccg atgccggcac
     9961 gcgcgagttg ctgggcgacg ccgttgccgg catcgaccag gtacacatct tcatctacga
    10021 ccaacgcata ggccgccggg aaccggttgg gcttgggcgt ggggccaccc gctgtaccga
    10081 gcaccaccaa tcgtgattcg ggtcccgacg ccacgacgcg cggcgcggcg gccagcaggc
    10141 cgctggccgc cagtgttgcc aggaatccgc ggcgatcaac gcgcatccgc gctcagccca
    10201 gtgcctgcgc caggtccgcg accaggtcgt cggcatcttc cagtcccacg gaaatccgca
    10261 acaggttctg cggcgagcgt gggttcggcc cttccaccga tgcgcgatgt tcgacgagcg
    10321 attcgcagcc gcccagcgac gtggcattgg tgaacacgcg cagcttgccg gccatcgcca
    10381 gcgcggcctc gcgcccgcca cgcacctcga tgctgagcat cgcgccgaaa tcgcgcatct
    10441 gtcggttcgc aacctcgtgt ccgggatgcg acgacaaccc cggccagttc acccgttcga
    10501 tcgcggtatg cgcggccagg aagtccacga ccttgcgcgc gttcgcgcaa tgcatggcca
    10561 tgcgcgcacc cagcgagcgg caaccgcgca acgtcagcca cgcactgaac ggcgccagca
    10621 cagcgccggt gacatgcagc cggtgcgcga ccttgcgggc aagctcgcca tcgtgtgcga
    10681 acaccagcgc gccgccgagc acatcgctat ggccgccgaa atacttggtg gtcgaatgca
    10741 tcaccacgtc cgcaccgagc gccagcgggc gctgcagcag cggcgtggcg aacgtgttgt
    10801 cgaccacgat caccgcgccg gccgcatgtc cgatgtcggc cagtgcggca atgtcgctga
    10861 ctttcatcaa cggattcgac ggcgtctcga tccacaacat cgacaccggc gtcgcgcacg
    10921 ccgcccgaac ggcgtcgagg tcgcccatgt cgatcacctc ggccacgagg ccgcgctcag
    10981 gcaggaactc ctggaacagc atgcgcaggc cgctgtagca gtcgtcgggg aacagcacgc
    11041 gggcgccgtt cggcaggcat tccagcagcg tggtcatcgc cgccatgccg gaggcgaaag
    11101 tgcaggcggt ctcgccgcct tccagcgccg ccagcgcctc gcgcaggcgg tcgttggtcg
    11161 ggttgccctc gcgctggtat tcgtagccgg cgacgcgctc gccggccggg ccgtggcgga
    11221 acgtcgtgct caggtgcagc ggcggcgcaa cggcgccggt agcgtcatcg ggtgcgttgc
    11281 cggcgtggac ggcgagggtg tcggggcgga gcgtcgtcat gccagcctca tgggtcgtga
    11341 cagggaaggg cgcggccgca cttgcggcaa tgccaggcgt cggcctcgtg gtcgatcagg
    11401 ccgcattcgg tgcaacgcac gttgttgcgg ctcgggcgca tggatcgcgc aagttccgcg
    11461 gtgtagatgc cggtggggac cgcgatgatg ctgtaaccga tgatgatcag gaccgaggtc
    11521 accaggcggc cgagcgtcgt ggccggtgcg atgtcgccga aaccgaccgt ggccatcgtc
    11581 accaccgccc agtacatgcc ggtggggatg ctggtgtagc cgtgcgccgg gccctcgatg
    11641 acatacatca tcgcgccgaa gatcacggtg atggtgagca aggtcgcgat gaacaccagg
    11701 atcttgcgcc ggctgcgcaa caaggcctgc accagcacgc cggcctcgct cgaatactcg
    11761 gccagcttca gcacgcggaa gatccgcagc aggcgcagga tgcggaccac ggccagcgaa
    11821 gtgcccgcgg ggaaaagcaa cgacagcagc gtgggcagga tcgccagcag gtcgatgatg
    11881 ccgaagaagc tggtggcgta gcgcagcggg cgcctgaccg tccacagccg caccaggtat
    11941 tcgatggcga acacaagggt gaagaaccat tcggccagcg acagcgggac atgccagcgt
    12001 tgcttgacgg ctggcacgct gtccagcatc accaccgcca cgctggcgag gatcgccacg
    12061 atcagccaca ggtcgaaatt tcgctccgcc gggctgtcgt gatggaagat cagccgatac
    12121 cagcggtagc gcgcgccttc ggtcgtggcc ggccaatgtt cgcgctcgaa cagactgcgt
    12181 ggtgcgtcgc gctggcgatc gtccgtctgc atgtcagagg gaacggaacg cctcgccggc
    12241 cgcgcgaatc gtgtcggcga tggccgcctc gtcgtgcgcg ctggacatga agccggcctc
    12301 gaacgcggag ggcgccaggt acacgccgcg ttccagcatc gcgtggaaaa agcggttgaa
    12361 cgcggcgctg tcgcaggcca tcgcctgcgc ataggtgtcg acgatgtcgt tgctgaagaa
    12421 catgccgaac atgccgccga cgcgttgcgt ggtgaacgcg ataccggcat cgcgcgccgc
    12481 ggcttccagc ccatcgcaca gcgcattcgt cttcgcttcg agtgcgtcgt gaaaccctgg
    12541 cgcctgcacc agctccagca tcgccaggcc tgcggccatc gccaccggat tgccgctcag
    12601 cgtgccggcc tggtagatcg ggcccgccgg cgcgatctgt tgcatcagtt cgcgacggcc
    12661 cccataggca cccaccggca tgccgccgcc gattaccttg ccgaaggtcg acaggtccgg
    12721 cgtcacgccg tagcgggcct gcgcgccacc cagcgcgacg cggaagccgg tcatcacttc
    12781 atcgaagacc agcaaggcgc cgtgtcgcgt gcacaggtcg cgcaggtgct gcaggtagcc
    12841 ttcgcgcggc ggcaggcagt tggcattgcc gaccaccggc tccacgatca ggcaggcaat
    12901 ctcgctgccg cattcctcga acagcctggt tgcgccgtcg aaatcgttgt aggtcagggt
    12961 gagcgtcaaa tcggccaatg ccttcggcac ccccggcgag gtcggcacgc cgaaggtgag
    13021 cgccccgctg ccggccttca ccaggaagga atcgccgtgg ccgtggtagc agccttcgaa
    13081 tttgacgatg cggctgcgcc cggtcgcgcc gcgcgccagg cgaatcgccg acagcgtggc
    13141 ctcggtgccg gagttgacca tgcgcaccat ctcgcagctc gggatcagcc gggtgatcgt
    13201 ctcggccatc gtcacttcca gcggattcgg ggtgccgaag ctcaggccgt tgcgcgcggt
    13261 cgcgatgacc gcatcgagca ccttcggatg gttgtggcca acgatcatcg ggccccatga
    13321 gcccacgtag tcgatgtagc ggttgccgtc ggcatcgaac aggtgcgggc cgtccgcgcg
    13381 ctgcacgaag aacggctcgc cgcccaccga cttgaacgcg cgcaccggcg agttcacgcc
    13441 gccgggcatc agcgcctggg cgcgggtgaa gagatcgtgc gagcgggcgt tgttcattcg
    13501 gattgggttc caggaattcc ggtatcgaaa caggagtggt aggcgcgtgc agcggccacc
    13561 gggtcggccg cgtcgaacac gccgctgatg acggcgacca ggtcggcgcc ggcggcgacc
    13621 agcgatggcg cattgtccgc ggtgatccca ccgatcgcca cgcgcggcag gccaaggtcc
    13681 gcgctgtcgc gcaacaggtc cagcgatgcg cgacgcgcat gcggcttggt cggagacgcg
    13741 aagaacgcac cgaatgcgag gtagcccgcg ccttcggcgg caaggcggcg ggcgcggtcg
    13801 atgtcgtcgt agcaggacac gccgatgatc gcgcgatcgc ccaggagcgt ccgcgccgat
    13861 gcgatgtcgc catcggcctc gcccaggtgt acgccatcgg catcgagttc cctggcaagc
    13921 gctggatcgt cattgacgat gaggggcaca ccagcgtggt tgcatgcgtg tcgcaggcgc
    13981 cggcctgcgt cgcgcaggga atcgacgtcc atcgccttgt tgcgcaactg caggcaggac
    14041 gcgaatgcca gcaatggcgt ggtgcgcgca agcagccgtg ccgggtcggg atcgtcgggc
    14101 gtgatcaggt aaaggccgcg tttcagtggg ttcgaaggca tcgcgcttcc gcaggccggc
    14161 gcagggtggg acaatcgatg ccgaccccgc cattatcgcc ccgatgaccg cctccgaatc
    14221 cgacgttccg taccgccagt ggatgtgcgt ggtctgcggc ttcatctacg acgaggcctt
    14281 gggcctgccg gaagacggca tcgctcccgg tacgcgctgg gaagacgtgc ccgatacctg
    14341 gacctgtccc gattgcgggg tgagcaagga cgatttcgaa ccggtcgagg cctgagccgg
    14401 gttatttcgc cggcttgatc gcagggctga agcgcatctc cgcgccatcc ttgaggccca
    14461 ggcgtgcggc ttcgccggca ttgagttcca gcacgtagcg cgctggcgca tcgctggggt
    14521 acgacgggca cgcatcgccc agcgaacacg tgggaacgtc gcgttgctgc gcgaccagct
    14581 tgcgttcgct gtcgaagtag aggatgtcga gcgggatctt cgtgttcttc atccagtacg
    14641 cctgcggctc ctcggcgtcg tggatgaaga gcatgccgtg gtcgctggcc atcgtgtcgc
    14701 ggaacatcag gccacgcgcg cgctcggcgt cgtcgtccga cacttcgaca acgtagcgct
    14761 ggccaccgat ctccacccat ggttgcgagg cgcccgcgca accggagatg ccgcaggaaa
    14821 cgagcaggac gagtgcgtac aagcgcatgc gaggagtcca tcgatttaag cagcgcgcac
    14881 catgcgcgcg aagcacggat tgcgtcaagg cacaagactt ccaagcggtt gaagcttgtt
    14941 gcatgatgcg cgcccgcttc accgccggcc tggccgcacg gatgaatcgg gcgaaaaaaa
    15001 tcatcacagg gtgttgacgg caacaaaaac ccttgtatga tg
//
LOCUS       DAOOCX010000056        15040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3592_length_15040_cov_8.074341, whole genome shotgun sequence.
ACCESSION   DAOOCX010000056 DAOOCX010000000
VERSION     DAOOCX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3592_length_15040_cov_8.074341"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PK399_06890"
     CDS             <1..306
                     /locus_tag="PK399_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011348771.1"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HPW12879.1"
                     /translation="FGKPALTMTLLGRLAARSDARVLFAWCERLPDTSPPRFALHVQA
                     APEAVGDADPAHAVAALNAGVEAIARRDFAQYQWTYKRYTLRPPGSGEANPYLDLYR"
     gene            303..815
                     /locus_tag="PK399_06895"
     CDS             303..815
                     /locus_tag="PK399_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010481194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPW12880.1"
                     /translation="MSDSPDAHAPDPAIDWHAPPARAVRLHATTNALWFAVIPLLAFA
                     IPLFAADGEQRLRMLAWYVGVTVAAAFAGAYFGARLRHQRWRLDAAGLWLRQGRLWWR
                     ETHVPNSRVQHVDLKHGPLERRFGLATLVVHTAAVHLSGITVRGLDEADAEQLRGTLA
                     KQLDDAGDAL"
     gene            819..2336
                     /locus_tag="PK399_06900"
     CDS             819..2336
                     /locus_tag="PK399_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPW12881.1"
                     /translation="MNDPAIDTAALPQDGLEHRLHPLSWLFVLLAHLWQFIPALVLLL
                     FAGRGDRNDLYGLIAVIVLVVLSLAQYFTYRYRLRSDAIVVRSGWLHRSVREIPYARI
                     HNVNLHQSLLHRLVGVAEVRLESAGGIKPEAQMRVLRLDQAEALEALVRQRGHAAGGD
                     APQADALEAPPLLAMSNGEVLRLGLISNRGMLVVAAAAGALAQAGDNVFGMLVERWGK
                     AVFGWFTGFAGEHAHDSPVAIGLAVASMVVVALVLVRLLSVLLAFLQYHGFTLREDDA
                     RIRVERGLLARYRSSAKRRRIQSWSLHEGVLHRWFKRRSLRVQTASGQRGEGAQRSLK
                     ELAPIATPAECDALIRHFLPEAGWDALAWQPLHRHAWWRIAVPGLLLVLVGTAALAWH
                     VGATGLLALALLPLQLWRAHRIADACGHACNDRLVAWRTGWLSKRWSFAEIGKIQAVR
                     LAQSPLDRRLGMASLLLDTAGASAFGAPLYLRQLPADVARDLSARLVAQLARRRV"
     gene            complement(2295..3914)
                     /locus_tag="PK399_06905"
     CDS             complement(2295..3914)
                     /locus_tag="PK399_06905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPW12882.1"
                     /translation="MIRSPALRDLRLPVLAWTAVALVSIFLHGPVPLYSTRSLAVAWE
                     MWDRGSWLVPLFNGAPYSHKTPLLPWLIHAGWLVGGVNDVWPRLLMVLLGGIVVAQAG
                     VLARRLYPQRVGIAANAAWAMAGFWYFFLFGLQVMYELPLAVAVLGGLIALCRRDGEA
                     WRPWWPGVALAIGFGLLAKGPVALLHLGVPLLAARWWHPAAQAEGRRFAKSALLALLG
                     GIVLFALWLLPALLLGDPEYREALLVTQTAGRIKDSFDHAEPWWWYAPTLLVLMAPWW
                     WQGWWWRQVPALWRDPANRFAWAWVIGMVLCFSLISGKQPYYLLPDFAGFALLLAVAA
                     DARPRLGAAPGVVVLVVALFLLAFPLYAGRITLPFPAQLGWSLPAAGGAMLLVALAML
                     RWRRLPVLAAGVLMACGLLHAGATPLIRDQFDFTPTAQLLGEAQRRGTPVAFLGEYQL
                     QFHFAGRLQVPLAALDEAGARTWASAHPQALIVVNWRDEWREPGDPQPLLQQRFRKRW
                     IQVWRAGDWRALPAQQIPLDPAASQLRYQPR"
     gene            3989..5014
                     /gene="galE"
                     /locus_tag="PK399_06910"
     CDS             3989..5014
                     /gene="galE"
                     /locus_tag="PK399_06910"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPW12883.1"
                     /translation="MADTILVTGGAGYIGSQMVKALRQAGFRPVVLDDLSSGHRSAVR
                     DEELHVGDVGDAAFVDRVLAEVRPAAVMHFAGFIQVGESVVDPGKYFRNNVTATQTLL
                     DAMRVHGIARFIFSSTAAIFGEPQYVPIDEAHPKAPINPYGRSKWFVEQLLEDYDHAY
                     GLKAVCLRYFNAAGADPDNEIGERHDPETHLVPLILQVASGRRPHIGVYGDDYPTADG
                     TCVRDYVHVSDLCDAHLLALRQLLGGAGSARYNLGNGNGFSVREVIDTARRITGHPIP
                     VVMEPRRAGDPPALVADAAAARAALGWTPRYADLQTIVAHAWACEQRIAAERDHPRPA
                     SDRNKSS"
     gene            complement(4975..6078)
                     /locus_tag="PK399_06915"
     CDS             complement(4975..6078)
                     /locus_tag="PK399_06915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW12884.1"
                     /translation="MTPVRVLHFVTGGFSGGATQVAVQLVNAAREGDAIEPLLVLRRK
                     RHADPSRIVELRKAGVPLELVAGWSHAATISALVSVCKRFKPDVLVAHGFSEHLWGRY
                     AGLLAKVPHLVHVEHNTRERYTAWRRAQARWLARRTDRIVGCSEGVRQALLAMGMPAE
                     RTIAIPNGVRLEPFADADAHPFAQRIPGIVMVARFSKQKDHATVLRALALLRARGLSP
                     SLLFAGGGKDLHRKPLERLADELGIAAQVRFLGVVRNVPELLMTHQLAVLGTHYEGMP
                     LALLEGMAAGCAVVGSAVPGVREVLEDGVDGLLVAESDPVAMADALERVLRDPGRAAR
                     MAATARQVALQRHGRALMNRRYEDLFLSLAGRG"
     gene            complement(6075..7724)
                     /locus_tag="PK399_06920"
     CDS             complement(6075..7724)
                     /locus_tag="PK399_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPW12885.1"
                     /translation="MTQTRRDTWLFWLFALLILGTGLGLRDPWPADEPRFALVAKQMV
                     ESGDWLFPHRGSELYSDKPPLFMWLQAAFLWLTGNLRIAFLLPSLLAALGTLWCVTDL
                     GARLWTRRIGLYAGWAVLLTIQFTWQAKKAQIDPLVVFWITLSCYGLLRHALRGPDWR
                     MWALGWAAAGLGTISKGVGVIALLMLLPIAFASLRGWPGVRLHARSGKFWLGPLAFLA
                     ACGVWLVPMLLAALGNGGPDYRAYVDDILLRQTVTRYANAWHHGQPPWYFIEVALTAW
                     LPTMLALPWAIPAWRRRLQRRDARYLLPLAWVLLVFVFFSIPSGKRDMYILPALPMLC
                     LALAPLLPGILRKRNAQRVLTGFVGVFSLALLGAGLAMLFGEPGFERRFMEGRDAAVA
                     HGLAAMFVATGAVGIGALLWCGRRRAQAALVAMLATLWVLYSLVGAPLLNDGSSSRGL
                     MREVGTVLGPDAQLGLVGWREQQLLMADRPAKDFGFKRDPALQFVDALAWQRQAPSSR
                     WLLVEGEGLPACVDKALSRDMGNANRRRWWLLPAAATRACR"
     gene            complement(7748..9031)
                     /gene="rsmB"
                     /locus_tag="PK399_06925"
     CDS             complement(7748..9031)
                     /gene="rsmB"
                     /locus_tag="PK399_06925"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPW12886.1"
                     /translation="MSAGVNARVSAARVLDVVLHRGRSLKATLAAALPALEDPRDRAL
                     VEAIVMCALRQRARFDAALAQWVAKPLEARDHELRALLLAGFAQLEIGLPAHAAVAAS
                     VDAARALGRPHQAGLVNAVLRRAQREGLPQAGSETAWPAWLAQRIRDDWPAQAEAIFA
                     GSAQEAPMWLRVNRRLQARDAYAAQLHDAGIATQVSPALADALRLDTALPVHELPAFD
                     TGAASVQDGAAQAVADALAPPNGARVLDACAAPGGKAAHLAERDPTLRITALDVDARR
                     VRRMQETFARIGLDIDTRTADALKPDAWWDGVPYDAILIDAPCSATGIVRRQPDILLH
                     RREADIDALVDTQAKLLDAAWPLLAAGGVLLYATCSILRAENAAQVDAFLARTPGARL
                     EPLDDAFGRDTGSGHQRLPGDDGMDGFFYARLSKA"
     gene            complement(9028..9945)
                     /gene="fmt"
                     /locus_tag="PK399_06930"
     CDS             complement(9028..9945)
                     /gene="fmt"
                     /locus_tag="PK399_06930"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPW12887.1"
                     /translation="MRIVFAGTPEFAVPCLRACARHGEVVAAYTQPDRPAGRGRGLQP
                     SPVKLEAVQRGIPVHQPENFRSAASKAELRALQPDLMVVVAYGLLLPQSVLDIPVHGC
                     WNVHASLLPRWRGAAPIQRAIEAGDDRTGVCLMQMEKGLDTGPVLLAQAVDIGPTETG
                     GQLHDRLSELGARVLGDALGLLRATIQLPPHPQAEEGATYARKLDKAEARLDWSLPAT
                     TLANKVRALNPWPMADAMLLGERVRIHGAVALDEVHGAQPGEVLRSGRDGIDIACGSG
                     VLRIRVLQREGGKAITAADYLNARRNPAA"
     gene            complement(10014..10523)
                     /gene="def"
                     /locus_tag="PK399_06935"
     CDS             complement(10014..10523)
                     /gene="def"
                     /locus_tag="PK399_06935"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004086447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPW12888.1"
                     /translation="MALLPILEFPDPRLRTKAVAVAPSELAEPALQRLFDDMFETMYD
                     APGIGLAASQVDVHKRFMVIDVTEDKSRPLVFVNPVIGARQGEQVYQEGCLSVPGIYA
                     DVTRSDVITVDALDRHGVPFTLEADGVLAVCIQHEMDHLDGKLFVDYLSPLKREMVRK
                     KLAKARRAA"
     gene            10745..11872
                     /locus_tag="PK399_06940"
     CDS             10745..11872
                     /locus_tag="PK399_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPW12889.1"
                     /translation="MLAVALLTVASFAAAVELRGDHPDTYVVKRGDTLWDIAGRFLQR
                     PWLWPEIWQANPQVKNPHLIYPGDVLSLAYLDRVAMQPGQRSESPVNAVNLSEIEPFL
                     KDLRIVDSLDGLPYVVGLEDDRLRSSRGQVAYIKGLEGIAPGQRYLVVRPEKRYRFVE
                     RAGLCCDLFQSEDLDARGDTFRGTGEFWTNAVFPSKNTEMLGYELITQSTGTVTRGEV
                     AGIQASTLLLDAEGRDVRVGDRLIPVEAQPYDLQFFPHPPKEQQKYGRLTVMSVADKY
                     RTGGPHDVIAISGGSREGIDNGTVFSIWRRGSSTVDTIKDGLDKDESLTLFESKVRLP
                     DEFAGHAMVFRTFNNVSYALVMSSVKPTMIGYELKHPDAPY"
     gene            11960..13090
                     /gene="dprA"
                     /locus_tag="PK399_06945"
     CDS             11960..13090
                     /gene="dprA"
                     /locus_tag="PK399_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPW12890.1"
                     /translation="MPITPEHASLLRLLLSGAPASALRRLLDTHGSAEASCRAGLRDW
                     TSHGLDADACKAIRTPDPQALARGEHWLDVDNHHLLGWRDDAYPPLLRRIASPPAMLF
                     VAGDPGLLWQPSVAVVGSRAATAGGCANAALFARALAQAGLCVASGLAAGIDAAAHHA
                     ALDATGSTVAVLGTGPDIAYPIQHRPLLDRLAAAAAVVSEHAPGTGPLRQYFPSRNRI
                     LAGMSLGTLVVEAADKSGALITARLAAEMGREVFAIPGSIHNPMARGCHRLLREGAQL
                     VESPAEVLDALAPLVVDLARALRGQLEPRRAIPTDAVQGAMDGDSDYNRLWQAIGHDP
                     TGMDDLVVRTGLTAATLSSMLLALELDGRIAAEHGRYARIGA"
     gene            13129..13617
                     /locus_tag="PK399_06950"
     CDS             13129..13617
                     /locus_tag="PK399_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HPW12891.1"
                     /translation="MKESILDVLLYLFEHYFANDAILTGDDFTALDSPLVGELTEAGF
                     SGAEIRKAFDWLDTLARQRPAVDQPRINGPTRIFHGSELDKLDVESRGFLLFLEQHGV
                     LDAGQRELVLDRAMALDQDELDLDDLKWVVLMVLFNQPGSEAAYAWMETQMFLDEPEP
                     VH"
     gene            13614..14333
                     /locus_tag="PK399_06955"
     CDS             13614..14333
                     /locus_tag="PK399_06955"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPW12892.1"
                     /translation="MNTVDPLPAEGQGSLYAPPTAAVEDVGDGFRVGGEVVYAGFWRR
                     AAALFIDSTLVGVAYYAVIIAGMLVVGFGRLSAFEGADAPGMGAIGGFLAVMYLAYPV
                     ISALYYVGMESSGTQATLGKMAVGIKVTDADGRRLGRGRALGRWASHLLCYLTLYIGY
                     IMAGFTERKRGLHDMVASTLVVDRWAFTDDPGRQRKGLGTVAIIVLVLGALLIVAYIG
                     IVLAIALPAYQTYLERAATGS"
     gene            14526..>15040
                     /locus_tag="PK399_06960"
     CDS             14526..>15040
                     /locus_tag="PK399_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005911395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPW12893.1"
                     /translation="MPKNLLVVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAV
                     DPEHGFAMRYDLIEKNEKHVEAIAKAAKGADNLFLATDPDREGEAISWHIAEILRERG
                     LVGDKPLQRVVFTEITPRAIKEAMQHPRAIASDLVDAQQARRALDYLVGFNLSPVLWR
                     KVQRGLSAGRV"
BASE COUNT         2355 a         5403 c         5191 g         2091 t
ORIGIN      
        1 ttcggcaagc cggcactgac gatgaccctg ctcggccggc ttgccgcgcg cagcgatgcg
       61 cgcgtgctgt tcgcctggtg cgagcggttg ccggatacct cgccgccgcg cttcgcccta
      121 catgtgcagg ccgcgccgga ggcggtgggc gatgccgatc cggcgcatgc cgtggccgcg
      181 ctcaatgccg gcgtcgaagc gatcgcgcgg cgggacttcg cgcagtacca atggacctac
      241 aagcgctata cgctgcgccc gccgggcagc ggcgaagcca atccctacct ggacctgtac
      301 cgatgagcga cagccccgac gcgcacgccc cggacccggc gatcgactgg cacgcgccac
      361 ccgcgcgcgc ggtgcgcctg cacgccacca ccaacgcgct ctggttcgcc gtgatcccgc
      421 tgctggcgtt cgcgatcccg ctgttcgccg cggatggcga gcaacggctg cgcatgctgg
      481 cctggtacgt gggggtgacc gtcgcggcag cattcgcggg cgcctatttt ggcgcgcgcc
      541 tgcgccacca gcgctggagg ctggacgctg ccggcctgtg gctgcgccag ggccgcctgt
      601 ggtggcgcga gacccacgtg ccgaacagcc gcgtgcagca cgtcgacctc aagcacgggc
      661 cgctggagcg ccgcttcggg ctggccaccc tggtcgtgca caccgctgcc gtgcacctga
      721 gcgggatcac cgtgcgcgga ctggacgaag ccgatgccga gcaacttcgc ggcaccctgg
      781 ccaagcaact cgacgatgcc ggcgacgcgc tgtaggcgat gaacgatccc gccatcgaca
      841 ccgccgcatt gccgcaggac gggctggaac atcggctgca tccgctgtcg tggctgttcg
      901 tcctgctggc gcatctctgg cagttcatcc ccgccctggt cctgttgctg ttcgccgggc
      961 gcggcgaccg caacgacctg tacggattga tcgcggtcat cgtgctggtg gtcctgtcgc
     1021 tggcgcagta cttcacctac cgctatcggc tgcgcagcga tgccatcgtg gtgcgcagcg
     1081 gctggctgca ccgcagcgtg cgcgagatcc cgtacgcgcg catccacaac gtcaacctgc
     1141 accagtcgct gctgcaccgc ctggtcggcg tggccgaggt gcgcctggaa tccgccggcg
     1201 ggatcaagcc ggaagcgcag atgcgggtgt tgcggctgga ccaggcggaa gcgctggagg
     1261 cactggtgcg ccaacgcggg catgccgccg gcggcgacgc gccgcaggcc gatgcgctgg
     1321 aagcgccgcc gttgctggcg atgtcgaatg gcgaagtcct gcggctgggg ctgatctcca
     1381 accgcggcat gctggtggtg gccgccgccg ccggtgcgct ggcgcaggcc ggcgacaatg
     1441 tgttcggcat gctggtcgag cgctggggca aggccgtgtt cggctggttc accgggttcg
     1501 ccggcgagca tgcgcacgac agccccgtgg ccatcggcct ggcggtggcc agcatggtgg
     1561 tggtcgcgct ggtgctggtg cgcctgctgt cggtcctgct cgccttcctg cagtaccacg
     1621 gcttcaccct gcgcgaggac gacgcccgca tccgcgtcga acgcggcctg ctggcccgct
     1681 accgctccag cgcgaagcgc cggaggatcc agtcgtggtc gctgcacgaa ggcgtgctgc
     1741 accgctggtt caagcggcgc agcctgcgcg tgcagacggc gtccggccaa cgcggggaag
     1801 gtgcgcagcg ttcgctcaag gaacttgccc cgatcgccac cccggcggag tgcgatgcac
     1861 tgatccggca cttcctgccc gaggccggct gggacgcgct ggcctggcaa ccgctgcatc
     1921 gacatgcctg gtggcgcatc gccgtacccg gcctgctgct ggtgctggtc ggcacggcgg
     1981 ccctggcctg gcatgtcggc gcgacgggct tgctcgcgct ggccttgctg ccgttgcaac
     2041 tgtggcgcgc gcatcgcatc gccgatgcct gcggccatgc ctgcaacgac cggctggtcg
     2101 cctggcgcac gggctggctg tcgaagcgct ggagcttcgc cgagatcggc aagatccagg
     2161 cggtccggct ggcgcaatcg ccgctggacc gccggctggg catggccagc ctgttgctgg
     2221 ataccgccgg ggcctcggcg ttcggcgcgc ccctgtacct gcggcagctg ccggccgacg
     2281 tggcccgcga cctgtcagcg cggctggtag cgcaactggc tcgccgccgg gtctagcgga
     2341 atctgctgcg ccggcaaggc gcgccagtcg cccgctcgcc agacctggat ccagcgcttg
     2401 cggaaacgct gctgcaggag cggctggggg tcaccgggct cgcgccattc gtcgcgccag
     2461 ttcaccacga tcaatgcctg cggatgtgcg ctggcccagg tccgcgcgcc ggcttcgtcg
     2521 agtgccgcca gtggcacctg caggcgaccg gcgaagtgga actgcaactg gtattcgccg
     2581 aggaacgcga ccggcgtgcc cctgcgctgg gcctcgccca gcagctgcgc ggtcggggtg
     2641 aaatcgaact ggtcgcggat cagcggcgtc gccccggcat gcagcaggcc acaggccatc
     2701 agcacgccgg cggccagcac cggcagccga cgccagcgca gcatcgccag cgccaccagc
     2761 agcatcgcgc cacccgccgc cggcagcgac cagcccagtt gcgcggggaa tggcagggtg
     2821 atgcgcccgg cgtatagcgg gaacgccagc aggaacaacg caacgaccag caccacgaca
     2881 cctggcgcgg cgccgagtcg cgggcgcgca tccgcggcga ccgccagcaa cagggcgaag
     2941 ccggcgaaat ccggcagcag gtagtacggc tgcttgccgc tgatcaggct gaagcacagc
     3001 accatgccga tcacccaggc ccaggcgaac cggttggcgg ggtcgcgcca cagggcgggc
     3061 acctgccgcc accaccagcc ctgccaccac cacggcgcca tcagcaccag cagcgtcggc
     3121 gcgtaccacc accacggctc ggcatggtcg aagctgtcct tgatccgccc cgcggtctgg
     3181 gtgaccagca gcgcctcgcg gtattcggga tcgcccagca gcagcgccgg cagcagccac
     3241 aacgcgaaca ggacgatgcc gcccagcagg gccagcagcg cggacttggc gaaacgccga
     3301 ccctcggctt gcgccgccgg atgccaccag cgcgccgcca gcagcggcac gcccaggtgc
     3361 agcaatgcga ccggaccctt ggccagcagg ccgaagccga tcgccagcgc caccccgggc
     3421 caccacggcc gccacgcctc gccgtcgcgc cggcacaggg cgatcagccc acccagcacc
     3481 gccaccgcga gcggcagctc gtacatgacc tgcaggccga acaggaagaa gtaccagaag
     3541 cccgccatcg cccatgcggc attcgcggcg atgccgacgc gttgcgggta caggcgtcgc
     3601 gcgagcacgc cggcctgcgc gactacgatg ccccccagca gcaccatcag caggcgcggc
     3661 cagacgtcat tgacgccgcc gaccagccag ccggcgtgga tcagccacgg cagcagcggc
     3721 gtcttgtgcg aatacggcgc gccgttgaac aacggcacca gccagctgcc gcggtcccac
     3781 atctcccacg ccaccgccag gctgcgggtg gaatacagcg gcaccggccc gtgcaggaag
     3841 atcgagacca gcgcgaccgc ggtccaggcc agcacgggaa gacggaggtc gcgcaaggcg
     3901 ggggaccgga tcatggcgcg caaggatagg gcacggacgc gcccgcatcg gcgacaatcg
     3961 aacgccccac ggcgcgcatc gattccccat ggcagacacc atcctggtca ccggcggcgc
     4021 cggctacatc ggctcgcaga tggtcaaggc gctgcgccag gccggcttcc gaccggtggt
     4081 gctggacgac ctcagcagcg gccatcgcag cgcggtgcgc gacgaggaac tgcacgtggg
     4141 cgacgtgggc gatgccgcgt tcgtcgatcg cgtgctggcc gaggtgcggc ccgcggcggt
     4201 gatgcatttc gccggcttca tccaggtcgg cgaatcggtg gtcgatcccg gcaagtactt
     4261 ccgcaacaac gtcactgcca cgcagacctt gctcgacgcg atgcgtgtgc acggcatcgc
     4321 ccgtttcatc ttctcgtcca ccgcggcgat cttcggcgaa ccgcagtacg tgcccatcga
     4381 cgaagcgcac ccgaaggcgc cgatcaatcc gtacgggcgc agcaagtggt tcgtcgagca
     4441 actgctggag gactacgacc acgcctacgg tttgaaagcg gtctgcctgc gctacttcaa
     4501 cgccgccggc gccgatcccg acaacgagat cggcgagcgc cacgatccgg aaacccacct
     4561 ggtgccgctg atcctgcagg tggcgtcggg ccgtcgcccg cacatcggcg tgtacggcga
     4621 cgactacccg accgcagacg gcacctgcgt gcgcgactac gtgcacgtgt ccgacctctg
     4681 cgacgcccac ctgctggccc tgcgccagtt gctgggcggt gccggcagcg cgcgctacaa
     4741 cctgggcaac ggcaacgggt tttcggtgcg cgaagtgatc gacaccgccc gccggatcac
     4801 cggccatccg atcccggtgg tgatggagcc gcgtcgcgca ggcgatccgc ccgcgctggt
     4861 ggcggatgcc gcggccgcgc gcgccgcact gggctggacg ccgcgctatg cggacctgca
     4921 gaccatcgtg gcccatgcct gggcctgcga gcagcggatc gcggcagagc gggatcaccc
     4981 gcgccccgcc agcgacagga acaagtcctc gtaacgccgg ttcatcagcg cgcggccgtg
     5041 gcgttgcagc gcgacctgtc gcgccgtcgc cgccatccgc gcggcgcgcc cgggatcgcg
     5101 caggacgcgc tccagcgcat cggccatcgc gaccggatcg gattcggcga ccagcaggcc
     5161 atcgacgccg tcttccagca cctcgcgcac gccgggcacc gcgctgccga ccacggcgca
     5221 gccggcggcc atgccctcca gcagggccag cggcatgcct tcgtaatggg tgccgagcac
     5281 ggccaactgg tgggtcatca acaactcggg gacgttgcgc accacgccca ggaagcgcac
     5341 ctgtgcagca atcccgagtt catccgccag tcgctccagc ggcttgcgat gcaggtcctt
     5401 gccgcccccg gcgaacagca gcgacggcga caggccgcgc gcgcgcaaca gggccagcgc
     5461 acgcagcacc gtcgcgtggt ctttctgctt ggagaagcgc gccaccatga cgatgccagg
     5521 gatgcgttgc gcgaacggat gcgcatcggc atcggcgaac ggttccaggc gcacgccgtt
     5581 cggaatggcg atggtgcgct cggccggcat gcccatcgcc agcagcgcct ggcgcacgcc
     5641 ttccgaacag ccgacgatgc ggtcggtgcg gcgcgccagc cagcgcgcct gcgcacgccg
     5701 ccacgccgtg tagcgctcgc gggtgttgtg ctcgacatgc acgaggtgcg gcaccttcgc
     5761 cagcaacccg gcgtaacggc cccacaggtg ttcgctgaag ccgtgcgcga ccagcacgtc
     5821 cggcttgaag cgcttgcaca cgctcaccag cgccgagatg gtcgccgcat gcgaccagcc
     5881 tgcgacgagc tccaacggca cgccggcttt gcgcaattcc acgatgcgcg agggatcggc
     5941 gtggcgcttg cgccgcagca ccagcaaggg ctcgatggcg tcgccctcgc gcgcggcgtt
     6001 gaccagttgc accgcgacct gggtggcgcc gccggaaaaa ccgccggtca cgaaatgcag
     6061 cacgcgcact ggcgtcatcg gcaggcccgg gttgcagcgg cgggcagcag ccaccagcgc
     6121 cgacggttgg cgttgcccat gtcgcgcgac agcgccttgt ccacgcacgc cggcaagccc
     6181 tcgccctcga ccagcaacca gcgcgacgag ggcgcctgcc gctgccacgc cagtgcatcg
     6241 acgaattgca gcgcggggtc gcgcttgaaa ccgaagtcct tggctggccg atcggccatc
     6301 agcaactgct gctcgcgcca gccgaccagg ccgagttgcg catcgggccc gagcaccgtg
     6361 ccgacctcgc gcatcaatcc acgcgaggac gaaccgtcgt tgagcaacgg cgcgccgacc
     6421 agcgagtaca gcacccacaa cgtggccagc atcgccacca gcgccgcctg cgcgcggcga
     6481 cggccgcacc acagcaaggc accgatgccg acagcgccgg tggcgacgaa catcgcggcc
     6541 agcccgtgcg cgacggcggc atcgcggcct tccatgaacc tgcgctcgaa gccgggctcg
     6601 ccgaacagca tcgccagccc cgcgcccagc aacgccagcg agaacacccc gacgaacccg
     6661 gtgagcaccc gttgcgcatt gcgcttgcgc aggatgcccg gcaacagcgg cgccagcgcc
     6721 aggcagagca tcggcagcgc cggcaggatg tacatgtcgc gcttgccgga cgggatgctg
     6781 aagaacacga acaccagcaa cacccacgcc agcggcagca gataacgcgc gtcgcgacgc
     6841 tgcaggcgac gacgccacgc cgggatcgcc cacggcagcg ccagcatggt cggcagccat
     6901 gcggtcagcg cgacctcgat gaagtaccag ggcggctggc cgtggtgcca ggcattggca
     6961 tagcgggtca cggtctgccg caacaggatg tcgtcgacgt aggcgcggta gtccggaccg
     7021 ccgttgccga gcgccgccag caacatcggc accagccaca ccccgcaggc ggcgaggaag
     7081 gccagcgggc ccagccagaa cttgccactg cgggcatgca accggacgcc cggccagcca
     7141 cgcaaggagg cgaacgcgat cggcagcagc atcagcagcg cgatcacgcc cacgcccttg
     7201 ctgatcgtgc cgagccccgc tgccgcccag cccagcgccc acatccgcca gtccgggccg
     7261 cgcagcgcat ggcgcaacag gccatagcag gacagcgtga tccagaacac caccagcgga
     7321 tcgatctgcg ccttcttcgc ctgccaggtg aactggatgg tgaggagcac cgcccaaccg
     7381 gcatacaggc cgatgcgccg cgtccacagc cgcgcgccga ggtcggtgac gcaccacagc
     7441 gtgcccagcg ccgcgagcag cgagggcagc aggaaggcga tacgcaggtt gccggtcagc
     7501 cacaggaacg ccgcctgcag ccacatgaac agcggcggct tgtccgaata cagctcgctg
     7561 ccgcgatgcg ggaacagcca gtcgccactt tccaccatct gcttcgcgac cagcgcgaag
     7621 cgcggctcgt ccgccggcca tggatcgcgc aggcccaggc cggtgccgag gatcaacagg
     7681 gcgaacagcc agaacaacca ggtgtcgcga cgggtttggg tcatcgcggg atcataacga
     7741 cgcgcggtta cgccttgctc agccgcgcat agaagaagcc gtccatgccg tcatcgccgg
     7801 gcagtcgctg gtggccactg ccggtgtcgc ggccgaaggc gtcatccagc ggctccagcc
     7861 gtgcgcccgg cgtgcgcgcc aggaacgcat cgacctgcgc cgcgttttcc gcgcgcagga
     7921 tcgagcaggt ggcgtagagc aacaccccgc ccgctgccag caacggccag gccgcgtcga
     7981 gcagcttcgc ctgggtgtcg accagggcat cgatgtcggc ctcgcgccgg tgcagcagga
     8041 tgtccggctg gcggcgcacg atgccggtgg cgctgcacgg cgcgtcgatc aggatcgcgt
     8101 cgtacggcac gccatcccac cacgcgtccg gcttgagcgc atcggcagtg cgcgtgtcga
     8161 tgtcgagtcc gatccgcgcg aacgtctcct gcatccgccg cacccgccgc gcgtccacgt
     8221 cgagcgcggt gatgcgcagc gtcggatcgc gttcggccag gtgcgcggcc ttgccgccgg
     8281 gcgcggcaca ggcatccagg acgcgcgcac cgttcggcgg tgccagcgca tcggccaccg
     8341 cctgcgcggc accgtcctgc accgaggccg cgccagtatc gaacgccggc aattcgtgca
     8401 ccggcagcgc ggtgtccagc cgcaaggcat cggccagcgc cggcgatacc tgggtggcga
     8461 tgccggcgtc gtgcaactgc gcagcgtagg catcgcgcgc ctgcaggcga cggttcaccc
     8521 gcagccacat cggcgcttcc tgcgcactgc cggcgaagat cgcctcggcc tgtgcgggcc
     8581 agtcgtcgcg gatgcgctgc gccagccaag cgggccacgc ggtctcgctg ccagcctgcg
     8641 gcaggccctc gcgctgcgcg cgacgcagca cggcattgac caggccggcc tggtgcgggc
     8701 gccccagcgc gcgcgccgca tccacgctgg ccgccaccgc ggcatgcgcg ggcaagccga
     8761 tttccagctg ggcgaagccg gcgagcagca acgcgcgcaa ttcgtggtcg cgggcctcca
     8821 gcggcttggc cacccattgc gcgagcgctg catcgaaacg cgcgcgctgg cgcaacgcgc
     8881 acatcacgat ggcctccacc agcgcgcggt cgcgcggatc ctccagcgcg ggcagggccg
     8941 ccgccagcgt cgccttcagc gagcgaccac ggtgcaggac cacgtcgagc acgcgcgccg
     9001 cactgacccg ggcattgacc ccggcgctca tgcggcgggg ttgcggcggg cgttgaggta
     9061 gtcggccgca gtgatcgcct tgccgccctc ccgctgcagc acgcggatcc gcaatacgcc
     9121 gctgccgcag gcaatgtcga tcccgtcgcg gccgctgcgc aggacctcgc cgggctgcgc
     9181 gccgtgcacc tcgtccagcg cgaccgcacc gtggatgcgc acgcgttcgc cgagcagcat
     9241 ggcgtcggcc atcggccacg ggttgagggc ccggaccttg ttcgcgagcg tggtcgccgg
     9301 cagcgaccag tccagcctgg cctcggcctt gtcgagcttg cgggcgtagg tcgcgccttc
     9361 ctccgcctgc ggatgcggcg gcaactggat ggtggcgcgc aacaggccca gcgcatcgcc
     9421 gagcacgcgc gcaccgagtt cgctgaggcg gtcgtgcagt tgcccgccgg tctcggtggg
     9481 accgatgtcg accgcctgcg ccagcaggac cggaccggta tccaacccct tttccatctg
     9541 catcaggcag acgccggtgc gatcgtcgcc ggcttcgatc gcgcgctgga tcggcgccgc
     9601 gccgcgccag cgcggcagca gcgaggcgtg cacgttccag cagccatgca ccgggatgtc
     9661 gagcaccgac tgcggcagca gcaggccata ggccaccacc accatcaggt ccggctgcag
     9721 cgcgcgcagc tcggccttcg aggcggcgct gcggaaattc tcgggctggt ggaccgggat
     9781 gccgcgctgc acggcttcca gcttgaccgg cgacggttgc aggccgcggc cgcgaccggc
     9841 tggccggtcc ggctgggtgt aggcggccac gacctcgcca tggcgcgcgc aggcgcgcag
     9901 gcagggcacg gcgaattccg gcgtgccggc gaagacgatc ctcaagcgcg gttctccaaa
     9961 cgtggacggc ggcgtggcga caggttcgcc ggtgaccgcc gatccggggc gggtcaggcg
    10021 gcgcggcgcg ccttggccag cttcttgcgg accatctccc gcttcagcgg gctgaggtag
    10081 tcgacgaaca gcttgccgtc caggtggtcc atctcgtgct ggatgcagac cgccaggacg
    10141 ccgtcggcct ccagggtgaa cggcacgccg tggcggtcga gggcatccac cgtgatcacg
    10201 tcgctgcgcg tgacgtcggc gtagatgccg ggcaccgaca ggcagccttc ctggtagacc
    10261 tgctcgccct ggcgggcgcc gatcaccggg ttgacgaaca ccagcggccg gcttttgtcc
    10321 tcggtcacgt caatcaccat gaagcgcttg tgcacgtcca cctggctggc cgccaggcca
    10381 atgcccgggg cgtcgtacat ggtctcgaac atgtcgtcga acagccgctg caaggcgggt
    10441 tcggccagtt cggagggggc aacggcgacc gccttggtgc gcaggcgcgg gtcggggaat
    10501 tcgaggatgg ggagcagggc catggcgggg cgtccgggac gtgatcgtcc gtatttgcga
    10561 cagtcttcgc gattgtaccc gcccgggact gagccgaggc ttgccagcgc aggcggtttg
    10621 tggactatag tgagtcggct tcagggggtt ttttaccaag ggaagcgggg agatggctgc
    10681 catgcttgaa cgtgtttctg agacgttgcg tcctgccgtg ccgcgtgcac gcggcgtacg
    10741 cacaatgctt gccgtcgccc tgctgacggt ggcgagtttc gccgccgcgg tggagttgcg
    10801 cggcgatcat cccgacacct acgtggtgaa gcgtggcgac acgctctggg acatcgccgg
    10861 tcgtttcctg cagcggccgt ggctgtggcc ggaaatctgg caggccaacc cgcaggtcaa
    10921 gaacccgcac ctgatctacc cgggcgacgt gctcagcctg gcctacctgg atcgcgtcgc
    10981 gatgcagccc ggccagcgca gcgagagccc ggtcaatgcg gtcaacctgt ccgagatcga
    11041 gccgttcctg aaggacctgc ggatcgtcga cagcctcgat ggcctgccct acgtggtcgg
    11101 cctggaagac gaccgcctgc gcagctcgcg tggccaggtt gcctacatca agggcctgga
    11161 aggcatcgcc cccggccagc gctacctggt ggtccgcccg gagaagcgct accgcttcgt
    11221 cgagcgcgcc gggctgtgct gcgacctgtt ccagagcgag gatctcgatg cccgcgggga
    11281 tacgttccgc ggcaccggcg agttctggac caatgccgtg ttcccgagca agaacaccga
    11341 gatgctcggc tatgagctga tcacccaatc caccggcacg gtgactcgtg gcgaagtggc
    11401 tggcatccag gcctcgaccc tgctgctcga cgccgagggc cgcgacgtcc gcgtcggcga
    11461 ccgcctcatc ccggtcgagg cgcagccgta cgacctgcag ttcttcccgc acccgccgaa
    11521 ggaacagcag aaatacggtc gcctgacggt gatgtcggtg gcggacaagt accgcaccgg
    11581 cggcccgcac gacgtgatcg cgatctccgg cggttcccgc gaaggcatcg acaacggcac
    11641 cgtgttctcg atctggcggc gcggcagttc caccgtcgac accatcaagg acggcctgga
    11701 caaggacgag tccctgaccc tgttcgaatc caaggtgcgc ctgccggacg agttcgccgg
    11761 ccatgcgatg gtgttccgca ccttcaacaa cgtcagctac gcgctggtga tgagcagcgt
    11821 caagcccacc atgatcggct acgagctgaa gcatccggac gcgccgtact gatcgcggct
    11881 tgaggaagcg gggtttcctg accccggcac gcgacggcgc ccgagggcgc cgtcgtcgtt
    11941 tgcggcggat gctggcggca tgccgatcac gcccgaacat gcctccctgt tgcgcctgct
    12001 gctcagcggt gccccggcca gcgccctgcg ccgcctgctg gacacccacg gcagcgccga
    12061 ggccagctgc cgcgccggcc tgcgcgactg gaccagccat ggcctggacg ccgatgcctg
    12121 caaggcgatc cgcacgccgg atccgcaggc actggcccgc ggcgagcact ggctcgacgt
    12181 cgacaaccac cacctgctcg gctggcgcga cgacgcctac ccgccgctgc tccggcggat
    12241 cgcatcccca ccggcgatgc tgttcgtcgc cggcgacccc ggcctgctgt ggcagccatc
    12301 ggtcgcggtc gtcggcagcc gggcggcgac tgccggcggc tgcgcgaacg cagccctgtt
    12361 cgcccgcgcg ctggcccagg cgggcctgtg cgtggccagc ggtctggcgg cggggatcga
    12421 tgcggccgcc caccacgccg cgctcgatgc caccggctcg acggtggcgg tgctcggcac
    12481 cgggccggac atcgcctacc cgatccagca tcgtccgctg ctggaccggc ttgccgccgc
    12541 cgcggcggtc gtcagtgaac atgcgccggg gaccggaccg ctgcgccagt atttccccag
    12601 ccgcaaccgg atcctggccg ggatgagcct gggcacgctg gtggtggaag ccgccgacaa
    12661 gtccggcgcc ctgatcacgg cccgcctggc cgcggagatg ggccgcgagg tgttcgccat
    12721 ccccggctcg atccacaacc cgatggcccg cggttgccac cgcctgctgc gcgagggcgc
    12781 ccaactggtg gagtcgccgg ccgaggtgct ggacgcgctg gcacccttgg tggtcgacct
    12841 ggcccgggcc ttgcgcgggc aactggagcc gcgtcgggcg ataccgaccg acgccgtcca
    12901 gggcgcgatg gacggcgact cggactacaa ccggttgtgg caggcgatcg gacatgaccc
    12961 aaccggtatg gatgacttgg tggtgcgtac cggattgacg gccgcgaccc tgtcctccat
    13021 gctgctggcg ctggagctcg acgggcggat cgcggcggaa cacggtcgct acgcccggat
    13081 cggggcctga cagcggttca cggcgcccga gggcgcaggc cgggggacat gaaagaaagc
    13141 atcctggatg tcctgctcta cctcttcgag cattatttcg ccaacgacgc gatcctcacc
    13201 ggcgacgact tcaccgcgct ggacagtcca ctggtcgggg aactgaccga agccgggttc
    13261 tccggcgccg agatccgcaa ggccttcgac tggctcgaca ccctggcgcg ccagcgcccc
    13321 gccgtcgacc agccgcggat aaacgggccg acccggattt tccacggcag cgaactcgac
    13381 aagctggacg tcgaatcccg cggcttcctg ctgttcctgg aacagcacgg cgtgctggac
    13441 gccggccagc gcgaactggt gctggaccgg gcgatggcgc tcgaccagga cgagctggac
    13501 ctggacgacc tcaagtgggt ggtgctgatg gtgctgttca accagccggg ctcggaagcg
    13561 gcctacgcgt ggatggaaac gcagatgttc cttgacgaac cggagccggt gcattgaaca
    13621 cggtcgatcc cctgccggcc gaaggccaag gctcgctcta cgccccgccg accgccgcag
    13681 tcgaggacgt gggcgacggc ttccgcgtcg gtggcgaggt ggtctatgcc ggattctggc
    13741 gtcgcgcggc ggcactgttc atcgattcca cgctggtcgg cgtcgcctac tacgccgtca
    13801 tcatcgccgg gatgctggtg gtggggtttg gcaggctgtc cgccttcgag ggcgccgatg
    13861 cgcccggcat gggcgcgatc ggcgggttcc tggccgtgat gtatctcgcc taccccgtga
    13921 tcagcgcgct ctattacgtg ggcatggagt cctcggggac gcaggccacg ctgggcaaga
    13981 tggccgtcgg catcaaggtc accgacgccg atggtcgccg cctgggccgg ggacgggcgc
    14041 tggggcgctg ggcctcccac ctgctgtgct acctcacgct ctacatcggc tacatcatgg
    14101 ccggcttcac cgagcgcaag cgcggcctgc acgacatggt ggcgagcacg ctggtggtcg
    14161 atcgctgggc cttcaccgac gacccgggac gccagcgcaa gggcctgggg acggtggcga
    14221 tcatcgtcct tgtcctgggg gccctgctga tcgtggccta catcgggatc gtgctggcga
    14281 tcgccctgcc ggcctaccag acctacctgg agcgcgctgc cacgggcagc tgaatcggct
    14341 cccggcggcg ccttgccggc cgccggccgg cggcaaaggc ttgacacggg ccccggcccg
    14401 tgagtccact gtaaaagaga cgaatcgagc gcccggggcc aaccaccccg ggcgttggct
    14461 tctgcggcgg cggctccgac ccggatgcca caccgcctcc accgacctcc cggaagctgc
    14521 gccgcatgcc caagaacctg ctcgtcgtcg aatcgcccgc caaggccaag acgatcaaca
    14581 aatacctcgg caaggacttc accgtcctcg ccagctacgg gcacgtccgt gacctggtgc
    14641 cgaaggaggg cgcggtcgat cccgaacacg gcttcgccat gcgctacgac ctgatcgaga
    14701 agaacgagaa gcacgtcgag gccatcgcca aggccgccaa gggcgcggac aacctgttcc
    14761 tggcgaccga cccggaccgc gagggcgagg cgatcagctg gcacatcgcc gagatcctgc
    14821 gcgagcgcgg gctggtcggc gacaagccgc tgcagcgggt ggtgttcacc gaaatcaccc
    14881 cgcgcgcgat caaggaggcg atgcagcacc cacgcgcgat cgccagcgac ctggtcgatg
    14941 cccagcaggc gcggcgcgcg ctggactacc tggtcggctt caacctgtcg ccggtgctgt
    15001 ggcgcaaggt ccagcgcggc ctctcggccg gccgcgtgca
//
LOCUS       DAOOCX010000057        14981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3614_length_14981_cov_10.024186, whole genome shotgun
            sequence.
ACCESSION   DAOOCX010000057 DAOOCX010000000
VERSION     DAOOCX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14981
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3614_length_14981_cov_10.024186"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..915)
                     /locus_tag="PK399_06965"
     CDS             complement(1..915)
                     /locus_tag="PK399_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPW12894.1"
                     /translation="MGGVPVSAASRQEPIALALGAGGARGLAQIGVIEALQARGLRIV
                     AIAGASSGALVGGACAAGKLDALRDWLLGTSRGAMLRLLDPGFGRPALFTGDRLLEKL
                     REVVGTPRIEDLPIDFTAVAVDLMRQREVWLRRGDLWDALRASFAIPGIFTPYTLGGR
                     ELVDGGLLAPLPITATRLSGAHRLVAVDMHGWPHSPPGELPSENDDTEGESAPGVIGR
                     WIGKHFGGDADGDGKPDHHIGLTETMSRSLDTMQAQIARVQLALDPPELLIRIPRDAC
                     QFYEFWRGEELIEIGRTQADKALDAAGY"
     gene            complement(915..1886)
                     /locus_tag="PK399_06970"
     CDS             complement(915..1886)
                     /locus_tag="PK399_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HPW12895.1"
                     /translation="MDRYERIIGLHRVLQASRRPVTVARLQDELGCSRATVYRDLAFL
                     RDGLMAPLIGDGEAGFSYDKNEADRFELPGLWLNSEELHALLAAQQLLSRSTGGMLSA
                     AMAPLQQRVEKLLDAHSGGKTWPVERVRVIPHRIRKMDEPAFRIVASSVLERRKLAFD
                     YRARSTDEKTRRTVSPQRLTHYRDNWYLDAWDETKQELRSFSIDRISGARFADGVARD
                     VADEELNAHLAGSYGIFSGAPKGWATILFSAKAARWVADEHWHSQQQGRFLPDGRYEL
                     KVPYSVPRELLMDVLHYGADAEIVEPKVLREQARAHLQLALGNYQAS"
     gene            1960..2715
                     /locus_tag="PK399_06975"
     CDS             1960..2715
                     /locus_tag="PK399_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12896.1"
                     /translation="MHPARHPLSRPLLAIALLAALAACKQSAPLPTSDSKDKPEPAAV
                     SQEAIAVAAAANAAPVASPIVPTVPTPLEVKAVVADKAAKDAVIAAMDKFKALRRYRI
                     TMMRGDGQATPTTTTVDYVAPDRYRVATTGAPTQVVIGDTLYTTDASGTRKSAAPADR
                     IGQWRDPAGLLAAGRDFTAEAGPRRFVFAMPASEYKLHVTRPVASNMSVWIGPKGLPI
                     KLESQGLADGKRVTTAIRYSHFDSEEIKIDAPK"
     gene            2865..>3670
                     /locus_tag="PK399_06980"
     CDS             2865..>3670
                     /locus_tag="PK399_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPW12897.1"
                     /translation="MTQPILNLSPSPGDEVKSTTCYMCACRCGIKVWLADGKIRYIQG
                     NRDHPVNQGVLCAKGASGIMQHYSPARLRKPLLRVGERGAGEFREIEWDEALDIATSW
                     LKPIRERNPDELAFFTGRDQSQALTGWWAQQFGTVNYAAHGGFCSVNMAAGGLYSVGG
                     SFWEFGEPDWEHTQLLMLWGVAEDHDSNPIKLGLGKLKQRGAKVIAVNPVRSGYGAIA
                     DEWIGIRPGTDGLFAFALIHELLRMDRIDLDYLVRYANAHWLVIDNPGGA"
     assembly_gap    3671..3770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3771..5739
                     /locus_tag="PK399_06985"
     CDS             <3771..5739
                     /locus_tag="PK399_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HPW12898.1"
                     /translation="IDISPAVVGTYTLPDGRNARPVFQLIVDRYLDPQYAPDAVSERC
                     GIPASTIRRIARELAEAAFDSKLELPIAWTDAWGREHATMQGRPVSMHAMRGISAHSN
                     GFHTCRALHLLQLLLGAVDAPGSFRYQPPFPKPIPPANRPGKARKPDGLLDAAPLGFV
                     HGPEDLVVDEHGQPRRIDHAYSWAYPLAAHGMMHTVIRNAWAGDPYKIDTLMMFMANM
                     SWNSAMNTGETMRWLTDKDEAGEYRIPRIIYSDAYASEMVAYADLVLPDTTYLERFDA
                     ISLLDRPISDADAASDAIRHPVFDPATQAGDDGQPRDVRGFQSVLIELGARIGLPGLL
                     NEDGSPKYRDYADYITRHERAPGVGLLAGWRGVDGSAEGKGAPNPAQLERYIEHGGFW
                     HSPVPASARYYKMANRDYLQWAQRFGFVPTAEPIVLQLYSETLQKFRLAAQGHGPHQP
                     PPEHRQRVATYFDPLPIWYEPFEHDQTSRTTFPLNAVTQRPMFMYHAWGSQNAWLRQI
                     ATRNYLYLHPDTGARHGIADEDWIEVTSHNGTISVQAKFAANVQPDTVWSWNAIGKRK
                     GAWTLAKDAPEATQGFLLNHLISDVTPKGDYANADPVTGQAAWFDLRVSIRKAGAETG
                     ESQPQFKPIGFSEANEAPLRYGASLRKRNVK"
     gene            5736..6374
                     /locus_tag="PK399_06990"
     CDS             5736..6374
                     /locus_tag="PK399_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HPW12899.1"
                     /translation="MSAALDWYFDVISPFAYLQWRRLRRDHADVALNPKPVLLAAILN
                     HVGQLGPAEIPQKRRHTYRIVLWQARQAGIPLVFPPAHPFNPLPALRLVLAAPDRVAA
                     IDAVFAHIWEHGKPADSIEALADVARGLGIDDPAAAIARDEVKRELLANGEEAMRLGV
                     FGVPTLKVRDELFWGFDATDLALAYRDDVNALDAEMRRVDAIPEAVQRRRPA"
     gene            6371..7114
                     /locus_tag="PK399_06995"
     CDS             6371..7114
                     /locus_tag="PK399_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPW12900.1"
                     /translation="MTALPPPSKKKLGLVIDLDTCVGCHACATSCKEWNAGGIAGPLT
                     DERPFAKDPQGVWFNRVHSYELEAEAATKQPAQTLHFPRSCLHCETPACVTVCPTGAS
                     YKRAEDGIVLVDEDKCIGCKLCSWACPYGAREYSEVEGVMKKCTLCVDRIYNQNLDEA
                     DRQPACVQACPTKARHFGDLGDPTSKVSLLVADRGGVALMPELDYKPVNRYLPPRPRR
                     TGADEADDAPASHDTGNLASRWLHRLLKR"
     gene            7157..7483
                     /locus_tag="PK399_07000"
     CDS             7157..7483
                     /locus_tag="PK399_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HPW12901.1"
                     /translation="MLVTTTPGVEGRRIVEYKGLVGGDAILGANMFRDMFAGLRDLVG
                     GRAGSYEKVLRGAKTQALEDMIEAALERGANAIVGVDLDYETVQVQDGGAMLMVSASG
                     TAVVIE"
     gene            7623..8564
                     /locus_tag="PK399_07005"
     CDS             7623..8564
                     /locus_tag="PK399_07005"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HPW12902.1"
                     /translation="MNPAFSVLIFTTLSGAGLGLWTWLGLRIALGEPPRDFQALGWIL
                     LLCYAGIAVAVGLLASFWHLGKPLRAWRAFSQWRTSWLSREGVLAVASFVPAAALLLM
                     LSDGTGPGRIDLGVRVVAGALALLSLATIACTAMIYASLKPIPAWRHPLVLPGYLLFA
                     LLTGGLPMLLSTWPNAEGVDRMLPWLLVAIVLAALVLKLAYWRAIDRTPLPQSRGDAI
                     GLPARTPSVFERPHTEANYITREMAFAVARRHASLLRIASVLLFAGLPLLCLFAWLQT
                     GGAGWPLPVAIGGALLGAFAERWLFFAQARHLVTLYY"
     gene            8610..9245
                     /locus_tag="PK399_07010"
     CDS             8610..9245
                     /locus_tag="PK399_07010"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HPW12903.1"
                     /translation="MLPLLWLAFTAPPITLDHVPAPILEPVVAQRLLAPPQCLAASCV
                     HGEWQLTGIPEIPAGQRRIDGDDLPGTGSVLHIADRKRRDWMKPQGSNDRVGMQVGVQ
                     ALNTEEASVAFSVDTAYRLQAYADDGTAGTGPVLRGQLEWRQALGERARLSQTTRVET
                     GQHGTYLRNSLQFNMKLQPQLTLSSGVEFRRDSDISGRNQTDATLNLRYAF"
     gene            complement(9306..10220)
                     /gene="hemC"
                     /locus_tag="PK399_07015"
     CDS             complement(9306..10220)
                     /gene="hemC"
                     /locus_tag="PK399_07015"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPW12904.1"
                     /translation="MTTLRIATRKSPLALWQTEHVADRLRAAHPGLQVELVPMSTRGD
                     EVLDRSLAAIGGKGLFLKELELAMQRGDADCAVHSLKDVPMELEPGFALPAILARADH
                     ADAFVSNRFDGIDALPRGARVGTSSLRRQAQLRALRPDLQLLDLRGNVNTRLAKLDGG
                     DYDAIVLACAGLQRLGFDARIRARLDAPQWLPAPAQGAIAIECRDDDPAIFALCAALD
                     DADTRTCVSAERAMNRALHGSCHVPVAAYARLDGDRLSLAGLVGSAADGRRVRAEAQG
                     QRDAADALGIEVAGLLLEQGARVLIDAE"
     gene            complement(10355..11104)
                     /locus_tag="PK399_07020"
     CDS             complement(10355..11104)
                     /locus_tag="PK399_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005915517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPW12905.1"
                     /translation="MGAEPMRVLIADDEPLARERLRAMLLAQPGVEVVAEAGDGHAAL
                     HACAEHHPDMILLDISMPGIDGLETARHLAEFEPRPVVVFCTAYDQHALSAFDAQAVD
                     YLVKPVRAERLAAALERVRTFAAGRVPAQAPEPDAARKRTHLCARLRGSLRLVPIEDI
                     RYLQAEEKYVVVHHARGEDLIEESLKALESEFGDRFVRIHRNCLVARGEIVELRRSGD
                     GHTQAVLRHGEHPLEVSRRCVAQLRETLKHL"
     gene            complement(11180..11863)
                     /locus_tag="PK399_07025"
     CDS             complement(11180..11863)
                     /locus_tag="PK399_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW12906.1"
                     /translation="MNSTVLLDAIEHETAADPRWCVLWLHGLGADGNDFAPIVPDLVR
                     RDWPAIRFVFPHAPVRAVTINNGARMRAWYDIRDFDLANRADHEGVAESVAQVDALIA
                     REATRGIPPERILLAGFSQGGAITLAAGLSRSPPLGGLVALSTYLPMGLDQARASLQP
                     AAASQPMFMGHGVFDPVVPVAGGEAAARAFRDFGFDVQWRRYPMQHSVCAEEIRDLGD
                     WMTARFAAG"
     gene            11952..12644
                     /locus_tag="PK399_07030"
     CDS             11952..12644
                     /locus_tag="PK399_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HPW12907.1"
                     /translation="MASNIGSVRARRIAAPWLLAALLACNAQAARLQVAVTGSGAPAA
                     DAVVSLHSPAAVAATRAGRATIDQRASQFVPRVTVVTVGTPVVFPNSDNVRHQVYSFS
                     PAKRFELPLYAGKPAAPVVFATPGVVELGCNIHDWMLAYVVVLDTPYHAITDARGQGR
                     IDAPAGTYTLRVWHPSLKGGTVHEERIVLADAPVERAVSLSLVATPEATAPADDKLRA
                     LQEKFRKLKDKR"
     gene            12641..>14981
                     /locus_tag="PK399_07035"
     CDS             12641..>14981
                     /locus_tag="PK399_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW12908.1"
                     /translation="MRVPGIGRLSFGARIVVVLVAILLLMQAATLLIVDVAVNRDVHR
                     QLAERIDVGDKVWRQLQRDRGQELTLAVGALASDFAFREALATGDASTVLSALHNHGQ
                     RMGADASLLLTVDGNLQTSTLDGDEAAQAVALAPLLEAARVQGSSAGVVQLDGRPYSL
                     AVVPVLAPRLIGWVAMGRLFGARETRDYRSLTGLDAAIGVREANRWRLVASSLDPDTA
                     HALVQLRATPREPVRISLADREFHAGTTPADPAHPGAIDVLLLASVDAAVLPYQHLKR
                     QIMLLATLAAVLALVVSVVVGRSVTRPVARLADAARRIEGGDYAESLPVVGDDELSDL
                     ANAFNRMQGGIAEREQRIRHQAQHDTLTGLPNRVHALALLEAALAQARTSDTRCAVLL
                     LDLDRFKEINDTLGHAFGDSVLEEVGRRLRNAIDEHDQLARLGGDEFLVLVAATDEAG
                     ALERAWSIIRALEAPLDLSERQAQVSIGASIGLALYPLHATDADALLRRADIAMYEAK
                     QAQRRVAVYQAGHDETHLRRIGLIGDLRTACERNEFRVFYQPKIDLRSQRVAHVEALL
                     RWQHPVLGNIAPDEFIPLAEHCGLIHEITGFVLAAGLQQASAWRNDGLDIGLAVNLSP
                     MDLLDARLPERVAGLLLAHRFPAQDLILEITESTVMRDVQSALVTMRALRALGVRLSI
                     DDFGTGHSSLAQLRSLPVDEIKIDKSFVMGLDSRPDDTVIVRSAIEIGHNMGLVVIAE
                     GVEQAGSLEILQRLHCDMVQGYFFSKPIPAEDFRRWHDDF"
BASE COUNT         2371 a         5249 c         5013 g         2248 t
ORIGIN      
        1 tcaatacccc gccgcgtcca gcgccttgtc ggcctgcgtg cggccgatct cgatgagttc
       61 ctcgccgcgc cagaattcgt agaactggca ggcgtcgcgg gggatgcgga tcagcagctc
      121 gggcggatcc agcgccagtt gcacgcgggc gatctgcgcc tgcatggtgt ccagcgagcg
      181 cgacatcgtc tcggtcaggc cgatgtggtg gtcgggcttg ccgtcgccgt cggcatcgcc
      241 gccgaaatgc ttgccgatcc agcgaccgat cacgccgggc gcgctctcgc cttcggtgtc
      301 gtcgttttcg cttgggagct cccctggtgg cgaatgcggc cagccgtgca tgtccaccgc
      361 gaccaggcga tgcgcaccgg agagtcgcgt cgcggtgatc ggcagcggcg ccaacaggcc
      421 gccatcgacc agttcgcgtc cgcccagcgt gtatggcgtg aagatgccgg gaatcgcgaa
      481 cgaggcgcgc agcgcatccc acaggtcgcc gcggcgcagc cagacttcgc gctggcgcat
      541 caggtcgacc gccaccgcgg tgaaatcgat cggcaggtcc tcgatgcgcg gcgtgccgac
      601 gacctcacgc agtttctcca gcaggcggtc gccggtgaac agcgcgggcc ggccgaagcc
      661 gggatcgagc aggcgcagca tcgccccacg actggtgccg agcagccagt cgcgcagcgc
      721 atccagcttg cccgcggcgc aagcgccgcc caccagcgcg ccactggatg cccccgcgat
      781 cgcgacgatg cgcaggccac gcgcctgcag cgcctcgatc acgccgatct gggccagccc
      841 gcgcgcgccg ccggcaccca gcgccagcgc gatcggttcc tggcgcgagg ccgcgctgac
      901 cggcaccccg cccatcacga cgcctggtag ttcccgagcg cgagctgcag gtgcgcgcgc
      961 gcctgttcgc gcaacacctt cggctccacg atctcggcgt cggcgccgta atgcagcacg
     1021 tccatcagca gttcgcgcgg caccgaatac ggcaccttca actcgtagcg accgtcgggc
     1081 aggaagcgac cctgctgctg cgaatgccag tgctcgtcgg ccacccagcg cgcggccttg
     1141 gcgctgaaca ggatcgtcgc ccagcccttc ggcgcaccgg agaaaatgcc gtagctgccg
     1201 gccaggtgcg cgttgagctc ctcgtcggcc acgtcgcggg ccacgccatc cgcgaagcgc
     1261 gcaccgctga tgcggtcgat ggagaaactg cgcagctcct gcttggtttc gtcccacgca
     1321 tccaggtacc agttgtcgcg gtagtgggtc agccgttgcg ggctgacggt gcggcgcgtc
     1381 ttctcgtcgg tcgagcgcgc gcggtaatcg aaggccagct tgcgccgctc cagcacgctc
     1441 gacgccacga tccggaatgc gggttcgtcc atctttcgaa tgcgatgcgg gatcacccgc
     1501 acgcgttcga ccggccaggt cttgccgccg gaatgtgcgt ccagcagttt ctcgacgcgt
     1561 tgctgcagcg gcgccatcgc cgccgacaac atgccgccgg tgctgcgcga cagcagttgc
     1621 tgcgcggcga gcagggcgtg caattcctcg gagttcagcc acaggccggg caactcgaag
     1681 cggtcggctt cgttcttgtc gtagctgaag ccggcttcgc catccccgat cagcggggcc
     1741 atcaacccgt cgcgcaggaa ggcgaggtcg cggtacacgg tggcgcgcga gcagccaagc
     1801 tcatcctgta gccgggccac ggtcaccgga cggcgggacg cctgcaacac gcggtgcagg
     1861 ccgatgatgc gttcgtaacg gtccatgcga cgattatgcc gccgatcgcc gagcttggcg
     1921 gccgcgcaac aaacacgcga cctgtcccga ggaacctgca tgcaccccgc ccgtcatccg
     1981 ttgtcacgcc ccctgctggc catcgccttg ctggctgcac tcgccgcctg caagcagtcc
     2041 gcgccgctgc ccacctccga cagcaaggac aagcccgaac cggcggccgt ctcccaggaa
     2101 gcgatcgccg tggcggcagc cgcgaacgcc gcacccgtgg cttcgcccat cgtgccgacg
     2161 gtgccgacgc ccctcgaggt caaggccgtc gtcgccgaca aggccgcgaa ggacgcggtc
     2221 atcgcggcca tggacaagtt caaggccctg cgtcgctacc gcatcaccat gatgcgcggc
     2281 gatggccagg cgaccccgac caccaccacg gtcgactacg tggcacccga tcgctaccgc
     2341 gtggcaacca ctggcgcgcc cacccaggtg gtgatcggcg acaccctcta caccaccgac
     2401 gcaagcggca ccaggaagag cgcggcacct gccgaccgca tcggccagtg gcgcgacccc
     2461 gccggcctcc tcgcggccgg acgcgatttc accgcagagg cagggcccag gcgtttcgtg
     2521 ttcgccatgc ccgcctccga gtacaagctc cacgtgaccc gcccggtcgc cagcaacatg
     2581 agcgtctgga tcggcccgaa gggcctgccc atcaagctgg aaagccaggg cctcgccgac
     2641 ggcaagcggg tcaccaccgc gatccgctat tcccacttcg acagcgaaga gatcaagatc
     2701 gacgcaccga agtaatcctt cgcttcggcc agtgcgcgcg gcacaatgcg cgccatggcc
     2761 gaacccgtcc tcaacccgtc gccttctccc ggcgacgagg tgaaaagcac gacgtgcgtg
     2821 tgtgcgctcg cccgcagggc ggcgcgtcgt gacggcagcc acccatgaca cagccgatcc
     2881 tcaacctgtc gccctctccc ggcgatgagg tcaaaagtac gacgtgctac atgtgtgcct
     2941 gccgctgcgg catcaaggtc tggctggccg acgggaagat tcgctacatc cagggcaacc
     3001 gagaccatcc ggtcaaccag ggcgtgctct gcgcgaaggg tgcgagcggg atcatgcagc
     3061 actactcgcc ggcgcggctg cgcaagccgc tgctgcgcgt gggcgaacgc ggtgccggcg
     3121 agttccgcga gatcgaatgg gacgaggcgc tcgacatcgc cacctcgtgg ctgaagccga
     3181 tccgcgaacg caacccggat gaactggcgt tcttcaccgg ccgcgaccag tcgcaggcgc
     3241 tgaccggctg gtgggcgcag cagttcggca ccgtgaacta cgcggcgcat ggcggtttct
     3301 gctcggtgaa catggccgcg ggtggcctgt attcggtcgg cggcagcttc tgggaattcg
     3361 gcgagcccga ctgggagcac acgcaactgc tgatgctgtg gggcgtggcc gaggaccacg
     3421 acagcaaccc gatcaagctc ggcctgggca agctgaagca acgcggcgcc aaggtcatcg
     3481 cggtcaaccc ggtgcgcagc ggctatggcg cgatcgccga cgaatggatc ggcatccgtc
     3541 ccggcaccga tggcctgttc gcgttcgcgc tgatccacga actgctgcgg atggatcgca
     3601 tcgacctgga ctacctggtg cgctacgcga atgcgcactg gctggtgatc gacaaccccg
     3661 gcggcgcgga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn catcgacatc
     3781 tcgcccgcag tcgtcgggac ttacaccttg cccgatggcc gcaacgcacg cccggtgttc
     3841 cagctgatcg tcgaccgcta cctcgatccg cagtacgcgc cggatgcggt cagcgaacgc
     3901 tgcggcatcc ccgcatcgac gattcgccgc atcgcgcgcg aactcgcgga agccgcgttc
     3961 gacagcaagc tcgagctgcc gatcgcgtgg accgatgcct ggggccgcga acacgccacg
     4021 atgcaggggc ggcccgtctc gatgcatgcg atgcgcggga tcagcgcgca cagcaacggc
     4081 ttccacacct gccgcgcgct gcacctgctg caactgctgc tgggcgcggt cgatgcgccg
     4141 ggttcattcc gctaccagcc gccgttcccc aagccgatcc cgccggccaa ccgccccggc
     4201 aaggcacgca aacccgatgg cctgctggat gccgcgccgc tgggtttcgt gcatggtccg
     4261 gaggatctgg tcgtcgacga acacggccag ccgcgacgga tcgaccatgc gtattcgtgg
     4321 gcgtatccgc tcgccgcgca cgggatgatg cacacggtga tccgcaacgc ctgggccggc
     4381 gatccgtaca aaatcgacac gctgatgatg ttcatggcca acatgagctg gaactcggcg
     4441 atgaacaccg gcgagaccat gcgctggctg accgacaagg acgaggccgg cgagtaccgc
     4501 atcccgcgga tcatctattc cgatgcgtat gcgtcggaga tggtcgcgta cgccgacctg
     4561 gtgctgccgg acacgactta cctcgagcgc ttcgatgcga tcagcttgct ggatcgcccg
     4621 atcagcgatg ccgacgcggc cagcgacgcg atccgccacc cggtgttcga tccggcgacg
     4681 caggccggtg acgacggcca gccacgcgac gtgcgcggct tccagtcggt gctgatcgaa
     4741 ctcggtgcgc gcatcggcct gccgggcttg ctcaacgaag atggttcgcc gaaatatcgc
     4801 gactacgccg actacatcac ccgccacgaa cgcgcgcccg gcgtgggcct gctggccggt
     4861 tggcgtggcg tggatggcag cgcagaaggc aagggcgcgc cgaatcccgc gcagttggag
     4921 cgctacatcg agcacggcgg tttctggcat tcgccggtgc cggcatcggc ccgctactac
     4981 aagatggcca accgcgatta cctgcagtgg gcgcagcgct tcggcttcgt gcccacggcc
     5041 gagcccatcg tgctgcagct gtattctgaa accctgcaga aattccgcct cgccgcgcag
     5101 ggccacggcc cgcaccagcc gccgccggaa catcgccagc gcgtggcgac gtatttcgat
     5161 ccgctgccga tctggtacga gccgttcgag cacgaccaga cctcgcgcac gacattcccg
     5221 ctcaatgccg tgacccagcg accgatgttc atgtaccacg cctggggcag ccagaatgcg
     5281 tggctgcggc agatcgccac gcgcaactac ctgtacctgc atccggacac cggcgcgcgg
     5341 catggcatcg ccgacgagga ctggatcgag gtcacgtcgc acaacggcac catctccgtg
     5401 caggcgaagt tcgccgccaa cgtgcagccg gacacggtgt ggagctggaa cgcgatcggc
     5461 aagcgcaagg gcgcgtggac gctggcgaag gacgcaccgg aagccaccca gggcttcctg
     5521 ctcaatcacc tgatctcgga cgtcacgccg aagggcgact acgcgaatgc cgacccggtg
     5581 accggccagg ccgcctggtt cgacctgcgc gtgtcgatcc gcaaggcggg cgcggaaacg
     5641 ggcgagagcc aaccgcaatt caagccaatc ggcttcagcg aagcgaacga ggcgccgttg
     5701 cggtatggcg catcgcttcg caaaaggaat gtgaaatgag cgcagcgctg gactggtatt
     5761 tcgacgtcat ctcgccgttc gcctacctgc agtggcgacg cctgcgccgg gaccatgccg
     5821 acgtagcgtt gaacccgaag ccggtgctgc tcgccgcgat cctcaaccat gtcggccagc
     5881 tcggccccgc cgagatcccg cagaagcgcc ggcataccta ccgcatcgtg ctgtggcagg
     5941 cgcgccaggc cggcatcccg ctggtcttcc cgcccgccca tccgttcaac ccgctgccgg
     6001 ccttgcgcct ggtcctggcc gcgccggatc gcgtggccgc gatcgatgcg gtgttcgccc
     6061 acatctggga acacggcaag cctgccgaca gcatcgaggc gctggcggat gtcgcacgcg
     6121 gactcggcat cgacgatccc gccgccgcca tcgcccgcga cgaagtcaag cgcgagctgt
     6181 tggcgaacgg cgaggaagcg atgcgcctgg gcgtgttcgg cgtgccgacc ttgaaggtgc
     6241 gcgatgaatt gttctggggt ttcgatgcca ccgatctcgc attggcctat cgcgatgacg
     6301 tgaacgcgct ggatgcggag atgcgccgcg tcgacgcgat ccccgaagcc gtacagaggc
     6361 gcaggcccgc gtgaccgcgc ttcccccgcc gtcgaagaag aagctcggct tggtcatcga
     6421 cctcgacacc tgcgtgggct gccatgcctg cgcgaccagc tgcaaggaat ggaacgccgg
     6481 cgggatcgcc gggccgctga ccgacgagcg tccgttcgcc aaggacccgc agggcgtgtg
     6541 gttcaaccgc gtgcacagct acgagctgga ggccgaagcc gcaacgaagc agcccgcgca
     6601 gaccttgcat ttcccgcgca gctgcctgca ttgcgagacg cccgcctgcg tgaccgtgtg
     6661 cccgaccggc gccagctaca agcgcgccga ggacggcatc gtcctggtcg acgaggacaa
     6721 gtgcatcggc tgcaagctgt gcagctgggc ctgtccctat ggcgcgcgcg agtacagcga
     6781 agtcgaaggg gtgatgaaga agtgcacgct gtgcgtggac cgcatctaca accaaaacct
     6841 ggacgaagcc gatcggcaac cggcctgcgt gcaggcctgc ccgaccaagg cgcggcattt
     6901 cggcgacctc ggcgatccga cctcgaaggt ctcgctgctg gtcgccgacc gcggcggcgt
     6961 ggcgctgatg ccggaactcg attacaagcc ggtcaatcgt tacctgccgc cacgcccgcg
     7021 ccgcaccggc gcggatgaag ccgacgacgc gccggcctcg cacgacaccg gcaacctcgc
     7081 atcgcgctgg ttgcaccgcc tcctgaaacg ctgacccacc cgcacaagga cacccacgat
     7141 ggccaaggac accccgatcc tcgtcaccac cacgcccggc gtcgaaggcc gtcgcatcgt
     7201 cgaatacaaa ggcctggtcg gcggcgacgc gatcctcggc gccaacatgt tccgcgacat
     7261 gttcgccggc ctgcgcgacc tcgtcggtgg tcgtgccggc agctacgaga aagtcctgcg
     7321 cggtgccaag acccaggcgc tggaggacat gatcgaagcc gccctcgagc gcggcgcgaa
     7381 tgcgatcgtc ggcgtggacc tggactacga gaccgtgcag gtgcaggacg gcggcgcgat
     7441 gctgatggtc tcggcgtcgg gcacggcggt ggtgatcgag tgatggtcgt tccgcgccgt
     7501 tcgatgttgc ctgctcgcag ccatccatgg cgcaacatgc ccgcctccgg aacgcccggg
     7561 gcaggcgtcc tgcctgcccg tccatgcgat gcgcgcggca atgccgcgca cgcgtgcatc
     7621 gcatgaaccc cgcgttttcc gtcctgatct tcaccaccct gtccggcgcc ggcctgggcc
     7681 tgtggacctg gctgggcctg cgcatcgcgc tgggcgaacc gccacgcgat ttccaggcgc
     7741 tgggctggat cctgctgctg tgctacgccg gcatcgcggt ggcggtgggc ctgctggcgt
     7801 cgttctggca cctgggcaag ccgctgcgtg cgtggcgggc gttttcgcaa tggcgcacct
     7861 cgtggctgtc gcgcgagggc gtgctggcag tcgccagctt cgtcccggcc gcggccttgt
     7921 tgctgatgct gtccgatggc acagggcccg gccgcatcga tctcggcgtg cgggtggtgg
     7981 caggtgccct ggcgctgctc agcctggcca cgatcgcctg caccgcgatg atctatgcct
     8041 cgctcaagcc gattccggcg tggcggcatc cgctggtgct gcccggttac ctgctgttcg
     8101 cgctgctgac cggtggattg ccgatgctgc tgtcgacctg gccgaatgca gaaggcgtgg
     8161 atcgcatgct gccctggctg ctggtcgcca tcgtgctggc cgcgctggtg ttgaagctcg
     8221 cgtactggcg cgccatcgac cgcacgccgt tgccgcagtc gcgtggcgat gcgatcggcc
     8281 tgccggctcg cacgccgtcg gtgttcgagc gtccgcatac cgaagccaac tacatcaccc
     8341 gcgagatggc cttcgcggtg gcacgccgcc acgccagcct gctgcgcatc gcatcggtcc
     8401 tgctgttcgc tggcctgccg ctgctgtgcc tgttcgcatg gctccaaacc ggaggcgcgg
     8461 gatggccgct gcccgtggcc atcggcggcg cgctgctggg cgcgttcgcc gagcgctggc
     8521 tgttcttcgc ccaagcccgg cacctggtca cgttgtacta ctgaccccgc gcggtagaat
     8581 ccgggccaac ccctcgcatc gattggccca tgctgccgtt gctgtggctg gcgttcaccg
     8641 cgccgccgat cacccttgac catgtgcccg cgccgatcct cgaaccggtc gtcgcgcagc
     8701 gcctgctcgc gccgccgcag tgccttgcgg cgagctgcgt gcatggcgaa tggcaactga
     8761 cgggcatccc ggagatcccc gccggccagc gccgcatcga tggcgatgac ctgcccggca
     8821 ccggcagcgt gctgcacatc gccgaccgca agcgccgcga ctggatgaag ccacagggca
     8881 gcaacgaccg cgtgggcatg caggtcggcg tgcaggcact gaataccgag gaagccagcg
     8941 tggcgttctc ggtggacacc gcctatcgcc tgcaggccta tgccgacgac ggcaccgccg
     9001 gcaccggccc ggtgctgcgc gggcaactgg aatggcggca ggccctgggc gagcgcgcgc
     9061 gactgtcgca gaccacccgc gtggagaccg gccagcacgg cacctacctg cgcaacagcc
     9121 tgcagttcaa catgaaacta cagccgcagc tgaccctgag ttccggcgtg gaattccgcc
     9181 gcgacagcga catcagcggt cgcaaccaga ccgatgccac gctgaacctg cgttacgcgt
     9241 tctgagaaaa gtggggtcgg agtggccttt tctgcgaagc gaaaaggcca ctccgacccc
     9301 acttttcatt cggcgtcgat cagcacgcgc gcgccctgtt ccaacagcaa ccccgccacc
     9361 tcgatgccga gcgcgtctgc ggcatcgcgc tggccttgcg cttctgcgcg cacgcgtcgc
     9421 ccatcggccg ctgaaccgac caaccctgca agcgagagcc tgtcgccatc gagccgcgca
     9481 tacgcggcca ccggcacatg gcagctgcca tgcagggcac gattcatcgc ccgctccgcg
     9541 ctgacgcagg tccgcgtgtc ggcatcgtcc agcgccgcgc acaacgcgaa gatcgcgggg
     9601 tcgtcgtcgc ggcattcgat cgcgatggcg ccctgcgcgg gcgcgggcag ccactgcggc
     9661 gcatccagcc gcgcgcggat gcgtgcgtcg aagccaagcc gctgcaggcc ggcgcaggcg
     9721 agcacgatgg cgtcgtagtc gccgccgtcg agcttggcca gccgcgtgtt gacgttgccg
     9781 cgcaggtcga gcaattgcag gtcaggccgc aacgcgcgca gctgcgcctg ccggcgcagc
     9841 gacgaggtgc cgacgcgcgc gccccgtggc aacgcatcga tgccgtcgaa gcgattgctg
     9901 acgaaggcat cggcatgatc ggcgcgggcg aggatcgccg gcaacgcgaa gcccggctcc
     9961 agttccatcg gcacgtcctt caacgaatgc accgcgcaat ccgcgtcgcc gcgttgcatc
    10021 gccagttcca gctccttgag gaacaggccc ttgccgccga tcgccgcgag cgagcggtcc
    10081 agcacctcgt cgccgcgcgt gctcatcggc accagttcga cctgcaggcc gggatgcgcg
    10141 gcgcgcaacc ggtcggcgac atgttccgtc tgccagaggg cgagcgggct cttgcgggtg
    10201 gcgatgcgca gcgtggtcat gcgggcatta tcgccgcttt ggctccctct cccgctggcg
    10261 ggagagggtt ggggtgaggg tggtcgcgcg catggctcca tcgccaccct catccgccct
    10321 tcgggcacct tctcccacaa ggggagaagg aaatctatag atgcttcaac gtctcccgca
    10381 gctgcgccac gcagcgccgg ctcacttcca gcggatgctc gccatggcgc agcaccgcct
    10441 gggtgtggcc atcgccgctg cggcgcagct cgacgatctc gccgcgggcg accaggcagt
    10501 tgcggtggat gcgcacgaag cggtcgccga actcggattc cagcgccttc agcgattcct
    10561 cgatcaggtc ctcgccgcgc gcgtgatgga cgaccacgta tttttcttcc gcctgcaggt
    10621 agcggatgtc ctcgatcggc accaggcgca ggctgccgcg caggcgcgcg cacagatggg
    10681 tgcgcttgcg cgcggcatcg ggctccggcg cctgtgcggg cacgcggccg gcagcgaagg
    10741 tgcgcacccg ctccagcgcc gcggccaggc gttcggcgcg caccggcttg accaggtaat
    10801 cgaccgcctg cgcatcgaac gcggacagcg cgtgctggtc gtaggcggtg cagaacacca
    10861 ccaccgggcg cggctcgaat tcggccaggt ggcgcgcggt ttccaggccg tcgatgccgg
    10921 gcatggagat gtccagcagg atcatgtcgg gatggtgttc ggcgcaggca tgcagggccg
    10981 cgtggccatc gccggcttcc gcgaccactt ccacgccggg ttgcgccagc agcatcgcgc
    11041 gcaggcgctc gcgggccagc ggctcgtcgt ccgcgatcag cacacgcatc ggctcggctc
    11101 ccatcgcggc gctccccggc agcatcaacc cgctgcccat ccgcaccgat agtagccccg
    11161 ccggcgcgga gggggaatct cagccggcgg cgaagcgcgc ggtcatccag tcgccgaggt
    11221 cacggatttc ctcggcgcag accgaatgct gcatcgggta acgccgccac tgcacgtcga
    11281 agccgaagtc gcggaacgca cgcgcggcgg cctcgccccc ggcgaccggc accacaggat
    11341 cgaacacgcc gtggcccatg aacatcggtt gcgacgccgc agccggctgc aacgaggcac
    11401 gcgcctggtc cagccccatc ggcaggtagg tggacagcgc gaccaagccg ccaagcggcg
    11461 ggctgcgcga caggccggcg gccagggtga tcgcgccacc ctgcgagaac ccggccagca
    11521 ggatccgttc cggcgggatg ccgcgggtgg cttcgcgggc gatcagtgca tccacctggg
    11581 ccaccgactc ggccacgcct tcgtggtcgg cgcgattggc caggtcgaag tcgcggatgt
    11641 cgtaccaggc ccgcatgcgc gcgccgttat tgatcgtcac cgcgcgcacc ggcgcatgcg
    11701 ggaacacgaa gcggatcgcc ggccagtcgc ggcgcacgag gtcgggcacg atcggcgcga
    11761 agtcgttgcc atcggcgccc aggccatgca accacagcac gcaccaacgt ggatcggcgg
    11821 cggtttcgtg ttcgatggcg tcgagcagga cagtcgaatt catcttcgtg agttggcgag
    11881 gatcatgctt aagtatccgg gatgggtgtc gcgatgacga ccccgcaggc accgctgcag
    11941 ggggcaacgg cttggcatcg aacatcggat ccgtgcgtgc acgccgcatc gccgcgccct
    12001 ggctgttggc agccttgctg gcatgcaacg cccaggcggc caggctgcag gtggcggtga
    12061 ccgggtcggg cgcgccggcc gcggatgcgg tggtcagcct gcattcgccg gcggcggtgg
    12121 cggccacccg cgccggacgc gccaccatcg accagcgcgc aagccagttc gtgccgcggg
    12181 tcacggtggt gacggtgggc acgccggtgg tgttccccaa cagcgacaac gtgcgccacc
    12241 aggtgtactc gttctcgccc gccaagcgct tcgagttgcc gctgtatgcg ggcaagccgg
    12301 ccgcgccggt ggtattcgcc acgcccggcg tggtcgagct gggctgcaac atccacgact
    12361 ggatgctggc ctacgtggtg gtgctcgaca cgccctacca cgcgatcacc gacgcgcgcg
    12421 gccagggccg catcgatgca cccgccggca cctacacgct gcgggtctgg cacccgagcc
    12481 tgaagggcgg caccgtgcac gaggagcgca tcgtgctggc ggacgcaccg gtcgaacgcg
    12541 cggtgtcgct gtcgctggtg gccaccccgg aagcgaccgc accggccgac gacaagctgc
    12601 gcgcgctgca ggaaaaattc cgcaagctca aggacaagcg gtgagggtgc cgggcatcgg
    12661 ccggctcagc ttcggtgcgc gcatcgtggt ggtgctggtc gcgatcctgc tgctgatgca
    12721 ggcggccacc ctgctgatcg tcgatgtcgc ggtgaaccgc gatgtgcatc ggcagctggc
    12781 cgagcgcatc gacgtgggcg acaaggtctg gcggcagttg cagcgtgacc gcggccagga
    12841 gctgacgctg gcggtgggcg cgctggcatc ggacttcgcg ttccgcgaag ccctggccac
    12901 cggcgatgcc tcgaccgtgc tgtccgcgct gcacaaccac ggccagcgca tgggggcgga
    12961 cgcctcgctc ctgctcacgg tggacggcaa cctgcagacc agcacgctgg acggcgacga
    13021 agccgcgcag gccgtcgccc tcgcgccgct gctggaggcc gcgcgcgtgc agggcagcag
    13081 tgccggcgtg gtccaactcg atggcaggcc ctattcgctg gcggtggtgc cggtcctggc
    13141 gccacggctg atcggctggg tggcgatggg tcgcctgttc ggcgcccgcg agacccgcga
    13201 ctaccgctca ttgaccggcc tggacgcggc catcggcgtg cgcgaggcga accgctggcg
    13261 gttggtggcc agcagcctgg accccgacac cgcgcacgca ctcgtgcagt tgcgcgcaac
    13321 gccgcgcgaa cccgtgagga tctccctggc cgatcgcgaa ttccatgccg gcaccacccc
    13381 ggccgatccg gcgcatccgg gcgcgatcga cgtgctcctg ctggccagcg tggacgccgc
    13441 ggtgctgccg taccagcacc tgaaacggca gatcatgctg ctggccacgc tggcggccgt
    13501 gctcgcgctg gtggtgtccg tggtcgtggg ccgcagcgtg acccgcccgg tggcgcggct
    13561 ggccgatgcc gcacgccgga tcgagggcgg cgactatgcc gagtccctgc ccgtggtcgg
    13621 cgacgacgag ctctccgacc tggccaatgc gttcaaccgc atgcagggcg gcatcgccga
    13681 acgcgaacaa cggatccgcc accaggccca gcacgacacc ctcaccggcc tgcccaaccg
    13741 ggtgcatgcg ctggccctgc tcgaagcggc gctcgcgcag gcgcgcacca gcgacacgcg
    13801 atgcgcggtg ctgctgctcg acctggatcg cttcaaggaa atcaacgaca ccctgggcca
    13861 tgccttcggc gacagcgtgc tggaggaagt cgggcggcgg ttgcgcaacg cgatcgacga
    13921 gcacgaccaa ctggcgcggc tgggcggcga cgagttcctg gtgctggtcg ccgctaccga
    13981 cgaagcgggc gcgctcgaac gcgcctggtc gatcatccgc gcactggaag cgccgctgga
    14041 cctgagcgag cgacaggccc aggtcagcat cggcgcgagc atcggcctgg ccctgtatcc
    14101 gctgcatgcg accgacgcgg acgcgctgct gcgccgcgcc gacatcgcca tgtacgaagc
    14161 caagcaggcg cagcgccggg tcgcggtcta ccaggccggc cacgacgaaa cccacctgcg
    14221 ccggatcggc ctgatcggcg acctgcggac ggcctgcgaa cgcaatgaat tccgggtgtt
    14281 ctaccagccg aaaatcgacc tgcgctcgca gcgcgtggcg cacgtggagg ccctgctgcg
    14341 ctggcagcat ccggtgctgg gcaacatcgc gcccgacgag ttcatcccgc tggccgagca
    14401 ttgcggcctg atccacgaga tcaccggctt cgtgctggcg gccggcctgc aacaggcctc
    14461 tgcgtggcgc aatgacggcc tggacatcgg cctggcggtg aacctgtcgc cgatggacct
    14521 gctcgacgcg cgcctgcccg agcgcgtggc tggcctgctg ctggcgcatc gcttcccggc
    14581 gcaggacctg atcctggaaa tcaccgaaag cacggtgatg cgcgacgtgc agtcggcgct
    14641 cgtcaccatg cgcgcgttgc gtgcgctggg cgttcgactg tcgatcgacg acttcggcac
    14701 cggccattcc tcgctggcgc agttgcgcag cctgccggtc gacgagatca agatcgacaa
    14761 gagtttcgtg atgggcctgg acagccgtcc cgacgacacc gtgatcgtgc gttcggcgat
    14821 cgagatcggc cacaacatgg gcctcgtcgt catcgccgag ggcgtggagc aggccggctc
    14881 gctggagatc ctgcagcgcc tgcactgcga catggtgcag ggctatttct tctcgaagcc
    14941 gatccccgcg gaggacttcc gccgctggca cgacgacttc c
//
LOCUS       DAOOCX010000058        14888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3646_length_14888_cov_8.306142, whole genome shotgun sequence.
ACCESSION   DAOOCX010000058 DAOOCX010000000
VERSION     DAOOCX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14888
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3646_length_14888_cov_8.306142"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PK399_07040"
     CDS             <1..633
                     /locus_tag="PK399_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPW12909.1"
                     /translation="GFGLCGIPEALIAALRDSGVKDLTVISNNAGVDGFGLGQLLTTR
                     QIRKMISSYVGENKEFERQYLGGELELEFNPQGTLAERLRAGGAGIPAFFTATGYGTI
                     VADGKETREIDGRHYVLETALKADVSLVKAWKADKAGNLVFRKTARNFNPAVAMAGKT
                     CVVEVEELVEVGEIDPDHVHLPGIYVQRIVVNATPEKRIEQRTIRQPEGN"
     gene            635..1261
                     /locus_tag="PK399_07045"
     CDS             635..1261
                     /locus_tag="PK399_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HPW12910.1"
                     /translation="MAWTRDQMAERAAKELTDGAYVNLGIGLPTLVANHIPEGMEVWL
                     QSENGLLGIGPFPTNDTVDADLINAGKQTVTAVPGASYFGSHDSFAMIRGGHIDLAIL
                     GAMEVTDKGDLANWMVPGKMVKGMGGAMDLVAGVQRVVVLMEHVTKSGSSKILPQCTL
                     PLTGVGVVDRIITELGVFDVTADGLKLVELADGVSREEAVEKTGVPLH"
     gene            complement(1389..3065)
                     /locus_tag="PK399_07050"
     CDS             complement(1389..3065)
                     /locus_tag="PK399_07050"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002114676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HPW12911.1"
                     /translation="MSQPEQDTVARESMEYDIVVVGAGPGGLATAIRLKQLATQAGRE
                     VSVCVLEKGSEAGAHILSGAIMDPKALTELFPDWKERGAPLKQAVTTDEFLFLDETGA
                     KATPHWLLPECFHNQGNYIVSLGAVVRWLAQQAEALGVEIFAGFAAAEVLYDDAGAVL
                     GVATGDMGIGKDGQPTDQYQRGMELHARYTIFAEGARGHLGRQLIAKYKLDAGKDPQS
                     YGIGIKELWQADPARHQPGLVVHTAGWPLDGATYGGSFLYHADDGKITVGFVVGLDYK
                     NPWLSPFNEFQRFKTHPAIRKHLEGGTRIGYGARAITAGGLLSLPRLVFPGGALVGCE
                     AGTLNASRIKGSHAAIKTGMLAAEAAFAALADGRQRDELSAYPIAFEQSWLHAELLQS
                     KNFKQWFKKGRTFATLMTGIEQWLLPKLGIRNPPWTIHRRKPDHACLEPAATQPKIDY
                     PKPDGVLTFDRLSSVFLSSTHHDEDQPSHLTLKDASVPVAVNLREYAGPEARYCPAGV
                     YEYIGDAGSERLQINAANCVHCKTCDIKDPTQNIVWVAPQGGGGPNYTGM"
     gene            complement(3151..3783)
                     /locus_tag="PK399_07055"
     CDS             complement(3151..3783)
                     /locus_tag="PK399_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HPW12912.1"
                     /translation="MSRLPRTLIAALLATLAGCSSLLGPKETPSIFAPDLATTANPAW
                     PSASWALGTVRPTAARILDGTRIAVSPVPGELQVYKGAMWARTPPEMLEETVLRMLED
                     SGKIPSVARQGSGIGADYRLVMDIRHFESRYDAGDGTPTAVIEVNAKLLHVPDQSVAG
                     SRTFRQSQAASGTEVALVADAFGQALGTLSREVAGWTLATGNEHQRQPHR"
     gene            complement(3780..4706)
                     /locus_tag="PK399_07060"
     CDS             complement(3780..4706)
                     /locus_tag="PK399_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPW12913.1"
                     /translation="METRANYVLIGGFTLLASIFLLLFALWAAKFSSDRNWREYQVVF
                     SEAVTGLTEGGSVQYNGISVGTVDKLSLVPDDARKVVALLKLKADTPVKVDTRAKLSQ
                     QGITGVPFILLSGGSPQAALLEPGPNDEIPIIRTEPSALQNIADTANRLVERMDHLLS
                     EENIRRISATLDNLEQATGSIAGQKEDIAALIVNAREVTDGLKLTVGKANGVIDGVDR
                     NLIARLPALMDRIDSTVAKLDSTAGNANALIAENRPAINSFANDGLAQLGPTLAELRR
                     LIRDLRNLSDRLEDRPASYILGRDAPKEFEPK"
     gene            complement(4722..5537)
                     /locus_tag="PK399_07065"
     CDS             complement(4722..5537)
                     /locus_tag="PK399_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW12914.1"
                     /translation="MTTDAGNARTLDGVVIGNAPLIRVRGLVNRFEEQTVHDGVDLDV
                     RRGEILGVVGGSGTGKSVLMRAILGLRRPQAGTIDVLGVDALSDDPVNRRHIERNTGV
                     LFQDGALFSSLTVGENVQVPLKEYYPDLPDSLRYELALLKIKLAGLPADALDKLPSQL
                     SGGMRKRAGLARALALDPPLLFLDEPTAGLDPIGAAAFDDLIRTLQQALGLTVFLITH
                     DLDTLYAICDRVAVLADRKLLAAAPVAELEALDHPWVQEYFNGPRGRAAREAR"
     gene            complement(5537..6658)
                     /locus_tag="PK399_07070"
     CDS             complement(5537..6658)
                     /locus_tag="PK399_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HPW12915.1"
                     /translation="MSKGPNHHPPTATVDPGSPGILRLRGRWTLRFASELGDALRATP
                     EGVTQVDAGGCERLDTLGVLQLLRFAERRGLDFQGFLFRDDQQPLVEAIEDVHDERPK
                     RKRDTGFQAALGRLGFAVVDNYREVMALIAFLGEVLVKLLRLFKEPSRFRVIATVHHM
                     EQVGLDAAPLVALLSFMVGAVIAFLGAMVLRDFGATIFVVELVNAAFLREFGVLLTAI
                     LLAGRTASAFTAQIGMMVNREEVDAIRVLGLDPIDLLVIPRVLALLVMLPLLTFIAMV
                     AGLLGGMAVGALNLDIPVVAYLARMHEMMEMRHFVVGMVKAPVFAMVIALIGCLEGLQ
                     TQGTAQSLGERTTSSVVQSISMVIIIDAFAALWFMQMDY"
     gene            6729..7979
                     /locus_tag="PK399_07075"
     CDS             6729..7979
                     /locus_tag="PK399_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPW12916.1"
                     /translation="MHAHKPLTEAGYTERVAFVVDVAQHLHAYGTTAQRLEGAVQSVA
                     RRLSLECEPWANPTGLILAFSDPARPAGESDTTRVIRLGLGDTDLYKLCEADRIAEEV
                     MAGHLDIPAGRAALRALDRPASWRGKAMQVLGFALASAAVAGLLRLPWLDIGTAGAIG
                     LLIGLLDIAAQRRPRLSEAFEAIAGLLAGTVAIAVSTLVAPLNLNTVIIASLIVLLPG
                     MALTNAMNELTSRQLVSGTARFAGALTTILKLTIGTAIALGIAGFAGIEPEVRALRPQ
                     PEWVVWSALLLGAFAFAVLFRAHRRDYPLVMASAIAGYLISRFGGEWVGAQAGLFLSA
                     LVTTAAGNLYARWRNRPGALVRLPGLIMLVPGSIALRGVITLVQAQDIGAGQDAAMLA
                     LNTLMALLAGLLFGNLLVSARRNL"
     gene            complement(8167..8562)
                     /locus_tag="PK399_07080"
     CDS             complement(8167..8562)
                     /locus_tag="PK399_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HPW12917.1"
                     /translation="MSVNINNLSSKELADLIVEANKRKKVLAKRKPASQVKAAVAKFL
                     KSTGWTFDELYGKSVAAKPAAAAGGASAPATVAKARKSTKGRSLGKVAAKYQNPANAK
                     ETWTGRGKQPRWLAAETAKGRKLEEFLIK"
     gene            complement(8662..10371)
                     /locus_tag="PK399_07085"
     CDS             complement(8662..10371)
                     /locus_tag="PK399_07085"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPW12918.1"
                     /translation="MRLSQFHLRTEKETPADAEVISHKLMLKAGMIRKLAAGLYTWSP
                     LGLRVMRKVEAVVREEMNRAGAIEMAMPSIQPKELWEETGRWAKFGPQLLKIRDRKEQ
                     DFCFTPTAEEAITDFARNELASYKQLPVNFYQVTTKFRDEIRPRFGVMRAREFVMKDA
                     YSFHIDDDSLHAEYRNMYDTYSRIFTRLGLKFRAVFADTGAIGGSASHEFHVLADSGE
                     DALAFSETSDYAANVELAEAVSPGERPAPSEALRDIDTPTQKTCEDVAALMGIPLART
                     AKSIAIMGRDNDGKDQFVLALVRGDHMANEIKLAKLPGLAEYRLATEAEIADHLGSEP
                     GFLGPLNAKKPIRVVADRSVAALADFVVGANRPGFHIAGVNWGRDLPEPEIADIRNVV
                     AGDASPDGQGVLGLARGIEVGHVFALGRKYSEAMKCTVLDANGKAVNPAMGCYGIGVS
                     RIVAAAIEQNHDANGIRWPAAMAPWNVAVCVINPKNDPAVSAAGEALYAELNAAGLDA
                     VLDDRGLRAGSMFADIELIGIPHRVVVSGRGLEAGTFEYRARDAAESENLDRETLLGK
                     LRG"
     assembly_gap    10411..10510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10511..10740)
                     /locus_tag="PK399_07090"
     CDS             complement(<10511..10740)
                     /locus_tag="PK399_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HPW12919.1"
                     /translation="MKQLGMLVLAIALGVAAWWWYTQEMPRRQRERAVAAEAAAKQAE
                     RADSLYRWRDDAGNLQITDEPPKGRKYERISRT"
     gene            complement(10737..11123)
                     /locus_tag="PK399_07095"
     CDS             complement(10737..11123)
                     /locus_tag="PK399_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HPW12920.1"
                     /translation="MHATRTVAAALALLLVAAPTLAQQQRVYQWKDANGVTHYTDLPP
                     TQAHKSRDIDNKGNAPEVATVKAAENPQCASSRANLQKLQANQTIGVDTDGDGKSDRN
                     LSSDERASQIELNQAAIKAYCPAAKP"
     gene            11225..11998
                     /gene="pssA"
                     /locus_tag="PK399_07100"
     CDS             11225..11998
                     /gene="pssA"
                     /locus_tag="PK399_07100"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPW12921.1"
                     /translation="MDPNHNATPPRPRGRGIYLLPNLLTTGGLFGGFFAIIAASQGRF
                     ETACIAIFVAAVLDGLDGRVARLTNTQSEFGVQYDSLADLVSFGMAPALVMYHWALVG
                     MKLDGVTAGKLGWLGAFLYAACAALRLARFNSQVAHVDKRWFIGLASPAAAGLMASFV
                     WTCNVFGWSGEELRYVALALTVCAGLLMVSRLRYNSFKGSGTGPKSDRVPFFTLLIAV
                     VVLIALWIEPPVTLLVAATLYAVSGPLLWFRRRRLDAVA"
     gene            12022..12249
                     /locus_tag="PK399_07105"
     CDS             12022..12249
                     /locus_tag="PK399_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12922.1"
                     /translation="MLAELGHVVYRPPVMAQGIQLDDAVLARLARAAGLAPEDFSRQF
                     GDLTPLAKLQGDARAKRALWPRLRAMRRGMA"
     gene            12270..12755
                     /gene="rimI"
                     /locus_tag="PK399_07110"
     CDS             12270..12755
                     /gene="rimI"
                     /locus_tag="PK399_07110"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPW12923.1"
                     /translation="MAAVTHLRPMRESDLDEIMRIEVRAYPFPWTRGIFRDCLHAGYP
                     MWVQEHDGEIVGYGVLSIGVEEAHVLNLCTAPGNEGQGLGRRMLHALLRVARGFGSQR
                     VFLEVRPSNPRAIALYDATGFNEIGRRPRYYPAANNGREDAIVMAMELLPDDINRMPP
                     L"
     assembly_gap    12782..12881
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12882..14887)
                     /locus_tag="PK399_07115"
     CDS             complement(<12882..14887)
                     /locus_tag="PK399_07115"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003490200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPW12924.1"
                     /translation="MTTLAPGYEPKSFESRLYQEWEASGAFAPQGDGPAYTILLPPPN
                     VTGTLHMGHAFQHTLQDALIRYHRMRGYRTLWQMGTDHAGIATEMVVSRNLAIAGQGE
                     TRDSLGRDKFIEKVWEWKQHSGDTIERQMRRLGASGDWSRSVFTMDPMASDAIVEAFV
                     RLHEQGLVYRGQRLVNWDPVLKTAISDLEVASEEENGHMWSIRYPLADGASYEHVEVD
                     ADGVEILRETRDYVVVATTRPETMLGDTAAMVHPEDPRYLALHGKFVELPLTGRRIPV
                     ITDDYVDKAFGTGVVKVTPAHDFNDYAVGQRHALPMINIFTDEAKVVSSRDDIAEKYR
                     GLDRFDARKLIVADLDATGLLVEIKPHRLQVPRGDRSGQVIEPFLTDQWFVKMDTLAQ
                     RGLELVESGEVKFVPPNWINTYRHWLENIQDWTISRQLWWGHRIPAWFDDAGNIFVGR
                     NEAEVRAAHDLGDAPLRQDPDVLETWFSSAMFPFSTQGWPNEDRMGELGFDVALPTSV
                     LVTGFDIIFFWVARMIMMTDQLVDRVPFRDVYITGLVRDKDGQKMSKSKGNILDPLDI
                     IDGITIDALVAKRTTGLMKPTDAPKIEKATRKEFPDGIPAFGADALRFTMAALAGPGR
                     DIKFDLLRAEGYKNFCNKLWNATRFALMNIGDASFEGAPQPKTDA"
BASE COUNT         2170 a         4841 c         5102 g         2575 t
ORIGIN      
        1 ggcttcggcc tgtgcggcat ccccgaggcg ctgatcgccg ccttgcgcga ttccggggtg
       61 aaggacctga cggtgatctc caacaatgcc ggcgtggacg gcttcggcct gggccagctg
      121 ctgaccacgc gccagatccg gaagatgatt tcgtcgtatg tcggcgagaa caaggagttc
      181 gagcgccagt acctgggcgg cgagctggaa ctggaattca acccgcaggg caccctggcc
      241 gagcgcctgc gcgccggcgg cgccggcatc ccggcgttct tcaccgccac cggctacggc
      301 accatcgtcg ccgacggcaa ggagacccgc gagatcgacg gccgccacta cgtgctggaa
      361 acggcgctga aggcggacgt gtcgctggtc aaggcctgga aggcggacaa ggccggcaac
      421 ctggtgttcc gcaagactgc gcgcaacttc aacccggcgg tggcaatggc cggcaagacc
      481 tgcgtggtgg aagtggagga actggtggaa gtgggcgaga tcgatcccga ccacgtgcac
      541 ctgcccggca tctacgtgca acgcatcgtg gtcaatgcca cgccggagaa gcgcatcgaa
      601 caacgcacca ttcgccagcc ggaggggaac tgagatggcc tggacccgtg accagatggc
      661 cgagcgcgcc gcgaaggaac tcaccgacgg cgcctacgtg aacctcggca tcggcctgcc
      721 gacgctggtg gccaaccaca ttcccgaggg catggaggtg tggctgcaga gcgagaacgg
      781 cctgctcggc atcggcccgt tccccaccaa cgacaccgtg gacgccgacc tgatcaatgc
      841 cggcaagcag accgtcaccg cggtgccggg cgccagctac ttcggcagcc acgattcgtt
      901 cgcgatgatc cgcggcggcc acatcgacct ggcgatcctc ggcgcgatgg aagtcaccga
      961 caagggcgac ctggccaact ggatggtgcc cggcaagatg gtcaagggca tgggcggcgc
     1021 gatggacctg gtggccggcg tgcagcgcgt ggtggtgctg atggagcacg tcaccaagtc
     1081 cggatcgtcg aagatcctgc cgcagtgcac gctgccgctg accggcgtgg gcgtggtcga
     1141 ccggatcatc accgagttgg gcgtgttcga cgtcaccgcc gatgggttga agctggtcga
     1201 acttgccgac ggcgtgagcc gcgaggaagc ggtcgagaaa acgggcgtgc cgcttcactg
     1261 aggtatcgca aggtccgccg gccggcgagc ccctttcgcg ttgtcgcata aaaacgaaaa
     1321 ggggacggag gtagttaagc gcgcttaact acctccgtcc ccttttcggg cggctgcctt
     1381 gtatcgggtt acatccccgt gtagttcggt ccgccgccgc cctgcggcgc gacccagacg
     1441 atgttctggg tcggatcctt gatgtcgcag gtcttgcagt gcacgcagtt ggcggcgttg
     1501 atctgcagtc gctcgctgcc cgcatcgccg atgtactcgt acacgcccgc cgggcagtag
     1561 cgcgcctcgg gaccggcgta ctcgcgcagg ttcaccgcga ccggcaccga tgcatccttc
     1621 agcgtcaggt ggctgggctg gtcttcgtcg tggtgggtgc tggacaggaa taccgagctg
     1681 aggcggtcga aggtcagcac gccatcgggc ttcgggtagt cgatcttcgg ctgggtcgcg
     1741 gccggttcca ggcaggcgtg gtcgggcttc cgccgatgga tcgtccacgg cggattgcgg
     1801 atgccgagtt tcggcagcag ccactgttcg atgccggtca tcagggtggc gaaggtgcgg
     1861 cctttcttga accactgctt gaagttcttc gactgcagca gttccgcatg cagccagctc
     1921 tgctcgaacg cgatggggta ggcgctcagt tcatcgcgct ggcggccgtc ggccagtgcc
     1981 gcgaaggcgg cctcggcggc cagcatgccg gtcttgatcg cggcgtggct gcccttgatg
     2041 cggctggcgt tgagcgtgcc ggcctcgcag ccgaccaatg cgccacccgg gaacaccagc
     2101 ctcggcaggc taagcaggcc gccggcggtg atcgcgcgcg cgccgtagcc gatgcgggtg
     2161 ccgccttcca ggtgcttgcg gatcgccgga tgcgtcttga agcgctggaa ttcgttgaac
     2221 gggctcagcc acggattctt gtaatccagg ccaaccacga aaccgacggt gatcttgccg
     2281 tcgtcggcgt ggtacaggaa cgagccgcca taggtggcgc catccagtgg ccagccggcg
     2341 gtgtgcacga ccagtcccgg ctggtgcctg gccggatccg cctgccacag ttccttgatg
     2401 ccgatgccgt aactctgcgg atccttgccg gcatccagct tgtacttcgc gatcagctgc
     2461 cggcccaggt ggccgcgcgc gccctcggcg aagatcgtgt acctggcgtg cagttccatg
     2521 ccgcgctggt actggtcggt gggctggccg tccttgccga tgcccatgtc gccggtggcg
     2581 acgcccagga ccgcgccggc atcgtcatac agcacttcgg cggcggcgaa gccagcgaag
     2641 atttccacgc ccaatgcttc cgcctgctgc gccagccagc gcaccaccgc acccaggctg
     2701 acgatgtagt tgccctggtt gtggaagcac tcgggcagca gccagtgcgg cgttgctttt
     2761 gcgccggttt catcgaggaa caggaattcg tcggtggtca ccgcctgttt cagcggagcg
     2821 ccgcgttcct tccagtccgg gaacagttcg gtcagcgcct tcggatccat gatcgcgccg
     2881 gacaagatgt gcgcgccggc ttcggatccc ttctcgagga cgcagaccga gacctcgcgg
     2941 cccgcctgcg tggccagttg cttcagccgg atcgcggtgg ccaggcctcc ggggcctgcg
     3001 cccacgacga cgatgtcgta ctccatcgac tcgcgcgcga cggtgtcctg ctccggttgg
     3061 ctcatctggc tctcctgtgg ctatccggga ttttcccggc tttgcctaga gcggacaatc
     3121 cagggagtgg aacgcgcggc gcgacgcgcc tcagcggtgt ggttgccgct ggtgttcgtt
     3181 gccggtggcc agggtccagc cggcgacctc gcgcgacagc gtgcccagcg cctggccaaa
     3241 ggcgtcggcg accagcgcca cctcggtgcc gctggcggct tgcgactggc ggaaggtgcg
     3301 gctgccggcg accgactggt cgggcacgtg cagcagcttg gcgttgacct cgatcaccgc
     3361 ggtcggcgtg ccgtcgccgg cgtcgtagcg cgactcgaaa tggcggatgt ccatcaccag
     3421 ccggtagtcc gcgccgatcc cgctgccctg gcgggcgacg gaggggatct tgccgctgtc
     3481 ctcgagcatc cgcagcacgg tttcctcgag catttccggc ggcgtgcgcg cccacatcgc
     3541 gcccttgtag acctgcagtt cgcccgggac cgggctgacc gcgatgcggg tgccgtccag
     3601 gatgcgcgcc gcggtgggac gcaccgtgcc cagcgcccag ctggccgacg gccatgccgg
     3661 gttggcggtg gtcgccaggt ccggggcgaa gatcgacggc gtttccttcg ggccgagcag
     3721 gctgctgcaa ccggcaagcg tggccagcag ggcggcgatc agggtgcggg gcaggcggct
     3781 catttgggtt cgaattcctt tggcgcgtcg cggcccagga tgtagctggc cggacggtct
     3841 tccaggcggt cggaaaggtt gcgcaggtcg cggatcaggc ggcgcagctc ggccagggtc
     3901 gggccgagct gggccaagcc gtcgttggcg aagctgttga tcgccggacg gttctcggcg
     3961 atcagcgcgt tggcgttgcc ggcggtggaa tcgagcttcg ccacggtgga atcgatccgg
     4021 tccatcagcg ccggcaggcg ggcgatcagg ttgcggtcga cgccgtcgat gacgccgttg
     4081 gccttgccca cggtgagctt gaggccgtcg gtgacttcgc gcgcgttgac gatcagcgcg
     4141 gcgatgtcct ccttctgccc ggcgatgctg ccggtggcct gctcgaggtt gtcgagggtg
     4201 gccgagatcc ggcggatgtt ctcctcgctc agcaggtggt ccatccgctc gaccagccgg
     4261 ttcgcggtgt cggcgatgtt ctgcagcgcg gacggctcgg tgcggatgat cgggatctcg
     4321 tcgttcggac ccggctccag cagggccgcc tgcggactgc cgccgctgag caggatgaac
     4381 ggcacgccgg tgatgccctg ctgcgacagc ttggcgcggg tatcgacctt caccggggta
     4441 tcggccttca gcttgagcag cgccaccacc ttgcgtgcgt cgtccgggac caggctgagc
     4501 ttgtcgaccg tgcccaccga gatgccgttg tattgcacgc tgccgccttc ggtcaggccg
     4561 gtcaccgctt cactgaagac cacctggtac tcgcgccagt tgcggtcgga cgagaacttc
     4621 gccgcccaca gcgcgaacag cagcaggaag atgctggcga gcagggtgaa gccaccgatc
     4681 agtacgtaat tggcacgggt ttccatcgtt ccttcctgtc cctagcgggc ctcgcgcgcg
     4741 gcgcgcccgc gcggtccgtt gaaatactcc tgcacccacg ggtggtcgag cgcttccagc
     4801 tccgccaccg gggccgcggc cagcaacttg cgatccgcca gcaccgccac ccggtcgcag
     4861 atagcgtata gcgtgtcgag gtcgtgcgtg atgaggaaca cggtcagccc cagcgcctgc
     4921 tgcagggtgc ggatcaggtc gtcgaacgcc gccgcgccga tggggtcgag gcccgcggtg
     4981 ggctcgtcca ggaacagcag cggcggatcc agcgccagcg cacgcgccag cccggcgcgc
     5041 ttgcgcatgc ccccggacag ttgcgagggc agcttgtcga gcgcgtcggc cggcaaccct
     5101 gcgagcttga tcttcaacag cgccagttcg tagcgcagcg aatccggcag gtccgggtag
     5161 tactccttca acggcacctg cacgttctcg cccacggtca gcgaagagaa cagcgcgccg
     5221 tcctggaaca gcacccctgt gttgcgctcg atgtggcggc ggttcaccgg atcgtccgac
     5281 agcgcatcca cgcccagcac gtcgatggtg cctgcctgcg gccgacgcag gcccaggatc
     5341 gcgcgcatca acaccgactt gccggtgccg gagccgccga ccacgccaag gatctcgccg
     5401 cggcgcacgt ccaggtccac gccgtcgtgc acggtctgct cctcgaagcg gttgaccagc
     5461 ccgcgcacgc ggatcagcgg tgcgttgccg atgaccacgc cgtcgagggt gcgcgcattg
     5521 ccggcgtcgg tcgtcatcag tagtccatct gcatgaacca cagcgcggcg aaggcgtcga
     5581 tgatgatcac catcgagatc gactggacca cgctcgatgt ggtgcgttcg cccagcgact
     5641 gcgcggtgcc ctgggtctgc agcccttcca ggcagccgat cagggcgatg accatcgcga
     5701 agaccggcgc cttgaccatg cccaccacga agtggcgcat ctccatcatc tcgtgcatgc
     5761 gcgccaggta cgcgaccacc gggatgtcca ggttcaaggc gccgaccgcc atgccgccca
     5821 gcaggccggc gaccatcgcg atgaaggtga gcaacggcag catcaccagc agggcgagca
     5881 cgcgcgggat caccagcagg tcgatcggat ccaggccgag cacgcggatc gcgtcgactt
     5941 cctcgcggtt gaccatcatg ccgatctgcg cggtgaacgc gctggcggtg cggccggcca
     6001 gcaggatcgc ggtcagcagc acgccgaact cgcgcaggaa cgcggcgttg accagttcca
     6061 ccacgaagat ggtggcgccg aagtcgcgca gcaccatcgc gcccaggaac gcgatcaccg
     6121 cgccgaccat gaacgacagc agggccacca ggggcgccgc gtccaggccg acctgctcca
     6181 tgtggtggac ggtggcgatc acccggaagc ggctcggttc cttgaacagg cgcagcagct
     6241 tgaccaggac ttcgccgagg aaggcgatca gcgccatcac ttcgcgatag ttgtcgacca
     6301 ccgcgaagcc gaggcgcccg agcgcggcct ggaagccggt atcgcgcttg cgcttggggc
     6361 gttcgtcgtg gacgtcctcg atcgcctcga ccagcggctg ctggtcgtcg cggaacagga
     6421 agccctggaa gtccaggccg cgacgttcgg cgaagcgcag cagttgcagc acgcccaagg
     6481 tgtccaggcg ctcgcagccg ccggcatcga cctgggtcac gccttccggc gtggcgcgca
     6541 gcgcgtcgcc gagttcactt gcgaagcgca acgtccagcg gccacgcagg cgcaggatgc
     6601 cgggggaccc cgggtcgacg gtggcggtgg gtggatggtg gttggggcct ttgctcatgc
     6661 gccgggcaag cttacgcaaa gcggggtgca tggtgtgaaa gggatttgcc gcatgcgatg
     6721 cttcccggat gcatgcgcac aagcccctga ccgaggccgg ctacaccgaa cgggtcgcct
     6781 tcgtggtcga cgtggcgcag catctgcacg cctacggcac caccgcgcag cgcctcgaag
     6841 gcgcggtgca gtcggtcgcg cggcggttgt cgctggagtg cgaaccctgg gcgaatccca
     6901 ccgggctgat cctggccttc agcgaccccg cgcgcccggc gggcgaatcc gataccaccc
     6961 gggtgatccg cctgggcctg ggcgataccg acctctacaa actgtgtgaa gccgaccgca
     7021 tcgccgaaga ggtgatggcc gggcacctgg acatccccgc ggggcgcgcg gcgttgcgcg
     7081 cgctggaccg gccagccagt tggcgcggca aggcgatgca ggtgctcggc tttgccctgg
     7141 cgtcggcggc cgtggccggg ctgctgcgat tgccgtggct ggacatcggc accgccggcg
     7201 cgatcggcct gctgatcggc ctgctggaca tcgctgcgca acggcgcccg cgcctgtccg
     7261 aggcgttcga ggcgatcgcc gggttgctgg ccggcacggt cgcgatcgcg gtctcgacgc
     7321 tggtcgcgcc gctcaacctc aacacggtga tcatcgcctc gctgatcgtg ctgctgccgg
     7381 gcatggcgct gaccaatgcg atgaacgagt tgaccagccg ccagctggtc tccggcacgg
     7441 cccgcttcgc aggcgcgctg accaccatcc tgaaactcac catcggcacc gcgatcgcgc
     7501 tgggcatcgc cggattcgcc ggtatcgagc cggaggtgcg cgcgctgcgc ccgcagcccg
     7561 aatgggtggt gtggagcgcg ttgctgctgg gcgcgttcgc cttcgcggtg ctgttccgcg
     7621 cgcatcgccg cgactatccg ctggtgatgg cgtcggcgat cgccggttac ctgatctcgc
     7681 gcttcggcgg cgaatgggtg ggggcgcagg caggcctgtt cctgtccgcg ctggtcacca
     7741 cggcggccgg caatctctat gcgcgctggc gcaaccgccc cggcgcactg gtgcgcctgc
     7801 ccggcctgat catgctggtg ccgggcagca tcgccctgcg cggagtgatc accctggtgc
     7861 aggcgcagga catcggcgcc ggccaggacg cggcgatgct ggcgctcaac acgctgatgg
     7921 cgctgctggc gggattgctg ttcggcaacc tgctggtgtc cgcgcgccgg aatctctgat
     7981 tcgaatagcc tcgctcgcga tgggattcgc gatgggattc ggcgagggac ggggtggggc
     8041 gcgacagcgc acgtcgagcg ccaagcgggc caccccgccc ggcgtcgcat cgggccatgc
     8101 ctgcaggcat tccccaaaca aaaacgccgg cgaacgccgg cgtttccaaa aacatcactg
     8161 accgatttac ttgatcagga attcttcgag cttcctgccc ttggcggtct ccgcggccag
     8221 ccagcgcggc tgcttgccac ggccggtcca ggtttccttg gcgttggccg ggttctggta
     8281 cttggcggcg accttgccca ggctgcggcc cttggtcgac ttgcgtgcct tcgccacggt
     8341 ggcgggcgcg ctggcaccgc cggccgcggc cgccggcttg gcggcgaccg acttgccgta
     8401 caactcgtcg aacgtccagc cggtgctctt caggaacttg gcgaccgcgg ccttcacctg
     8461 gcttgccggc ttgcgcttgg ccagcacctt cttgcgcttg ttggcctcga cgatcaggtc
     8521 ggccagttcc ttggagctga ggttgttgat gttgacgctc atgctgcgtg gttcctcgat
     8581 ggaaaatgcg ggatcgggtt gccggacccc gcatattaac ttccgtgatt aacgctggcc
     8641 aataatccgg aaaccgatgt ttcaaccacg gagtttcccg agcagggttt cccggtcgag
     8701 gttttccgat tccgccgcat cgcgcgcgcg gtattcgaag gtgccggctt ccagcccgcg
     8761 gcccgacacc accacgcgat gcgggatgcc gatcagctcg atgtcggcga acatcgagcc
     8821 cgcgcgcagg ccacggtcgt cgagcacggc gtcgaggccg gcggcgttca gttcggcgta
     8881 cagcgcttcg ccggcggcgc tcacggccgg gtcgttcttc gggttgatca cgcagaccgc
     8941 gacgttccag ggcgccatcg ccgccggcca gcggatgccg ttggcgtcat ggttctgttc
     9001 gatcgcggcg gcgacgatgc gcgacacgcc gatcccgtag cagcccatcg ccggattgac
     9061 cgccttgcca ttcgcgtcga gcaccgtgca cttcatcgcc tcggaatact tgcggcccag
     9121 cgcgaacacg tggccgacct cgatgccgcg ggccaggccc agcacgccct ggccgtccgg
     9181 cgaggcatcg cccgcgacca cgttgcggat gtccgcgatc tcgggttccg gcaggtcgcg
     9241 gccccagttc accccggcga tgtggaagcc cggcctgttg gcaccgacca cgaagtcggc
     9301 cagcgcggcc acgctgcggt cggcgaccac gcggatcggc ttcttcgcat tgagcgggcc
     9361 gaggaagccc ggctcgctgc cgaggtggtc ggcgatctcc gcttcggtgg ccagccggta
     9421 ctcggccagg cccggcagct tggccagctt gatctcgttg gccatgtggt cgccgcgcac
     9481 cagcgccagc acgaactggt ccttgccgtc gttgtcgcgg cccatgatgg cgatcgactt
     9541 cgcggtgcgc gcgagcggaa tccccatcag tgctgccacg tcctcgcagg tcttctgggt
     9601 cggcgtgtcg atatcgcgca gggcctcaga cggtgcgggg cgttcgccgg gcgacaccgc
     9661 ttcggccagc tccacgttcg ccgcgtagtc cgaggtttcc gaaaaggcga gtgcgtcttc
     9721 gccggaatcc gccagcacgt ggaactcgtg cgaggcgctt ccgccgatcg cgccggtgtc
     9781 ggcgaacacc gcgcggaact tcagccccag gcgggtgaag atgcgcgagt aggtgtcgta
     9841 catgttgcgg tattcggcgt gcagcgagtc gtcgtcgatg tggaaggaat aggcatcctt
     9901 catcacgaac tcgcgcgcgc gcatcacccc gaagcgcggg cggatctcgt cgcggaactt
     9961 ggtggtgacc tggtagaaat tgaccggcag ctgcttgtag ctggccagtt cgttgcgggc
    10021 gaaatcggtg atcgcctcct ccgcggtcgg ggtgaagcag aaatcctgct ccttgcggtc
    10081 gcggatcttc agcagttgcg ggccgaactt cgcccagcga ccggtttcct cccacagctc
    10141 cttcggctgg atcgacggca tcgccatctc gatcgcgccg gcgcggttca tttcctcgcg
    10201 gaccaccgcc tcgaccttgc gcatcacccg caggcccagc ggcgaccagg tgtacaggcc
    10261 ggcggccagc ttgcggatca tgccggcctt gagcatcagc ttgtggctga tgacttcggc
    10321 gtcggcggga gtttcctttt cggtgcggag gtggaactgc gagagacgca tgggggcatt
    10381 cgcgagcgga aagtccgcat tttgccatgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn gtccggctga tccgctcgta cttgcggcct ttcggtggct cgtccgtgat
    10561 ctgcaggttg ccggcatcgt cgcgccagcg atacagcgag tccgcgcgct cggcctgctt
    10621 tgccgccgct tccgccgcga cggcgcgttc gcgctggcgc cgcggcatct cctgcgtgta
    10681 ccaccaccag gcggccacgc ccagggcgat ggccagtacc agcatgccca gctgcttcac
    10741 ggcttggcgg cggggcagta ggccttgatc gccgcctggt tcagttcgat ctgggaggcg
    10801 cgctcgtcgc tgctcaggtt gcggtcggac ttgccatcgc catcggtatc cacgccgatg
    10861 gtctggttgg cctgcagctt ctgcaggttg gcgcgggagc tggcgcactg cgggttttcg
    10921 gccgccttga ccgtggccac ctcgggcgca ttgcccttgt tgtcgatatc gcggctcttg
    10981 tgcgcctgcg ttggcggcag gtcggtgtag tgggtcacgc cattggcgtc cttccactgg
    11041 tagacacgtt gttgctgggc cagggtgggc gcggccacca gcaacagggc aagtgcggcg
    11101 gcgacggtgc gggtggcgtg catggcgtac tccgggggaa tcccgcgccg attgcagcac
    11161 cgccacgcag ccaccgcaag tccggcggct tcggcgccgg cgggataggc cctacactgg
    11221 ctggatggac ccgaaccaca acgccacccc gccacgcccg cgcggccgcg gcatctacct
    11281 cttgcccaac ctgctcacca cgggggggct cttcggtggt ttcttcgcga tcatcgcggc
    11341 ctcgcaaggg cgcttcgaga ccgcctgcat cgccatcttc gtggcggcgg tgctggacgg
    11401 gctggacggc cgcgtcgccc ggctgaccaa tacccagagc gaattcggcg tgcagtacga
    11461 ctcgctggcc gacctggtca gcttcgggat ggcaccggcg ctggtgatgt accactgggc
    11521 cctggtgggg atgaagctgg acggcgtcac cgccggcaag ctgggttggc tgggcgcgtt
    11581 cctctatgcc gcctgcgcgg cactgcggtt ggcccgcttc aacagccagg tcgcgcatgt
    11641 ggacaagcgc tggttcatcg gcctggccag ccctgcggcg gcggggctga tggccagttt
    11701 cgtctggacc tgcaacgtgt tcggctggag cggggaagaa ctgcgttacg tcgccctggc
    11761 gctgaccgtt tgcgccggcc tgctgatggt cagccggctc cgctacaaca gcttcaaggg
    11821 cagcggcacc gggccgaagt cggatcgggt gccgttcttc accctgctga tcgcggtggt
    11881 ggtgctgatc gcgttgtgga tcgagccgcc ggtgaccctg ctggtggccg ccaccctgta
    11941 tgccgtgtcc gggccgctgt tgtggttccg ccggcgtcga ctggacgcgg ttgcgtgagt
    12001 tgggatccgt tccagcgtgc ggtgctggcc gaactggggc atgtggtcta tcggccgccg
    12061 gtcatggccc agggtattca actggacgat gccgtgttgg cgcggctggc acgcgccgcc
    12121 ggcttggcgc ctgaggattt cagccgccaa ttcggcgatc tcacgcccct ggcgaagctg
    12181 cagggcgatg cccgcgccaa gcgcgcgctg tggccgcgcc tgcgggcgat gcggcggggc
    12241 atggcatgaa cgcgcagcgc acggacgcga tggcggccgt gacgcacctg cggccgatgc
    12301 gcgagtccga cctggacgag atcatgcgca tcgaagtacg cgcctacccg ttcccgtgga
    12361 cgcgcggcat cttccgcgac tgcctgcacg ccggttaccc gatgtgggtg caggagcacg
    12421 acggcgagat cgtgggctac ggcgtgctca gcattggcgt ggaggaagcg cacgtgctca
    12481 acctgtgcac cgcgcccggc aacgaagggc aggggctggg ccggcgcatg ttgcacgcct
    12541 tgctgcgggt cgctcgcggc ttcggttcgc agcgcgtttt cctggaagtg cgaccgtcca
    12601 acccgcgcgc gatcgcgctg tacgacgcga ccgggttcaa cgagatcggc cgccgcccgc
    12661 gctattaccc cgccgccaac aacggccgcg aggacgcgat cgtgatggcg atggagttgc
    12721 tccccgacga catcaacagg atgccgccgc tgtagcggtc cgagatggaa aaccggggtc
    12781 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcatccgtc ttcggctgcg
    12901 gcgcgccttc gaagctggcg tcgccgatgt tcatcagcgc gaagcgcgtc gcgttccaca
    12961 gcttgttgca gaaattcttg tagccctcgg cgcgcagcag gtcgaacttg atgtcgcggc
    13021 cggggccggc cagcgcggcc atggtgaagc gcagcgcgtc ggcaccgaat gcggggatgc
    13081 cgtcggggaa ttccttgcgc gtcgccttct cgatcttcgg cgcgtcggtg ggcttcatca
    13141 gcccggtggt gcgcttggcg accagggcgt cgatcgtgat gccgtcgatg atgtcgagcg
    13201 ggtcgaggat gttgcccttc gacttcgaca tcttctggcc gtccttgtcg cgcaccaggc
    13261 cggtgatgta gacgtcgcgg aacggcacgc gatcgaccag ttggtcggtc atcatgatca
    13321 tccgcgccac ccagaagaag atgatgtcga agccggtgac cagcaccgag gtcggcagcg
    13381 cgacatcgaa gccgagctcg cccatcctgt cttcgttcgg ccagccctgg gtgctgaacg
    13441 ggaacatcgc cgaggagaac caggtctcca gcacgtccgg gtcctgccgc agcggcgcat
    13501 cgcccaagtc atgcgcggcg cgcacttcgg cttcgttgcg gccgacgaag atgttgccgg
    13561 catcgtcgaa ccacgccgga atgcggtggc cccaccagag ctggcggctg atcgtccagt
    13621 cctggatgtt ttccagccag tggcggtagg tattgatcca gttcggcggc acgaacttca
    13681 cctcgcccga ttcgacgagc tccaggccgc gctgtgccag cgtgtccatc ttcacgaacc
    13741 actggtcggt caggaacggc tcgatcacct ggccgctgcg gtcgccgcgc ggcacctgca
    13801 ggcgatgcgg cttgatctcg accaacaggc cggtggcgtc gaggtcggcg acgatcagct
    13861 tgcgcgcgtc gaaacgatcc aggccgcggt atttttcggc gatgtcgtcg cgcgacgaaa
    13921 ccaccttcgc ctcgtcggtg aagatgttga tcatcggcag cgcgtggcgc tggccgacgg
    13981 cgtagtcgtt gaagtcgtgc gcgggcgtga ccttgaccac gccggtgccg aaggccttgt
    14041 cgacgtagtc gtcggtgatc accgggatgc ggcgaccggt cagcggcagt tccacgaact
    14101 tgccgtgcag ggcgaggtaa cgcgggtctt ccggatgcac catcgccgcg gtatcgccga
    14161 gcatggtttc cgggcgggtg gtggcgacga ccacgtagtc gcgggtttcg cgcaggatct
    14221 cgacgccgtc ggcatcgacc tccacatgct cgtagctcgc accatccgcg agcgggtagc
    14281 ggatcgacca catgtggccg ttctcttcct cgctggcgac ttcaagatcg gagattgcgg
    14341 tcttcagcac cggatcccag ttgaccaggc gctggccgcg atagaccagg ccctgctcgt
    14401 gcaggcgcac gaaggcttcg acgatcgcgt cgctcgccat cggatccatg gtgaacacgc
    14461 tgcgcgacca gtcgccgctg gcgccgaggc ggcgcatctg ccgttcgatg gtatcgccgg
    14521 agtgctgctt ccactcccag accttttcga tgaacttgtc gcggcccagc gagtcgcggg
    14581 tttcgccctg gccggcgatg gcgaggttgc ggctcaccac catctcggtg gcgatgccgg
    14641 cgtggtcggt gcccatctgc cacagcgtgc gatagccgcg catgcggtgg tagcggatca
    14701 gcgcgtcctg cagggtgtgc tggaacgcgt ggcccatgtg caacgtgccg gtgacgttcg
    14761 gtggcggcag caggatggtg taggccggac cgtcgccctg cggggcgaac gcgccgctgg
    14821 cttcccattc ctggtacagg cgggattcga aggacttggg ttcgtagcct ggggcgaggg
    14881 tggtcatg
//
LOCUS       DAOOCX010000059        14648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3741_length_14648_cov_7.819091, whole genome shotgun sequence.
ACCESSION   DAOOCX010000059 DAOOCX010000000
VERSION     DAOOCX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14648
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3741_length_14648_cov_7.819091"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /gene="tal"
                     /locus_tag="PK399_07120"
     CDS             complement(<1..893)
                     /gene="tal"
                     /locus_tag="PK399_07120"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HPW12925.1"
                     /translation="MTRQLEQLRQHSVVVADTGDIEAIARFKPIDATTNPSLLLKAAA
                     LPAYASHIEAAVAAARGDDKVGDAGDRLAVAIGGEILKLVPGRVSTEVDARLSFDTEA
                     TIAKAHKLVQLYADAGIGTERLLIKVASTWEGIRAAERLERDGIQCNLTLLFSFAQAV
                     ACAEAGVFLISPFVGRILDWHLANGMATPATPQDDPGVQSVQRIWRYYKQHGYETVVM
                     GASFRNTGEVLALAGCDRLTISPDLLAALEQSDAAVEPALVDDGTRAAPPARLDEAAF
                     RWQHNEDAMATDKLADGIRKFA"
     gene            complement(996..3197)
                     /locus_tag="PK399_07125"
     CDS             complement(996..3197)
                     /locus_tag="PK399_07125"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017167545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPW12926.1"
                     /translation="MPAPIPVLRVALPVPLPRLFDYLPPTGCDAGTVAVGQRLRVPFG
                     SREVCGIVIGHGHAEPGVELRQALALPDPDPLLEGELLASLQWLAGYLHAPLGEVLAT
                     ALPAALRRGEPAADTAAYGWVLTEAGRTALPAMRSGKPKALATLLIHVRAEDWLDDEM
                     PGWRASLRGLRERGLVERSEMKVGSESPFQRKGDSDPTFFLNDEQRVAADAIRAADGF
                     AAFLLDGVTGSGKTEVYLDAIADCLARGRQALVLVPEIGLTPQALARFRKRLGVPVHA
                     LHSGLNDNERARVWLAAARGQARVIVGTRSAVFVPLPEAGIVIVDEEHDGSYKQFDGI
                     RYHARDVAIVRARALGVPAVLGSATPSLESLHNAQSGRYAHLRLRHRAGNAKAPHVRV
                     LDVRKRPLHAGLGEDALLAIRGALDAGGQVLVFRNRRGYAPVLLCHDCGWSAHCPRCS
                     TPDKPTPMTVHAHGRRLQCHHCGYRKPSPLACPDCASLGLQPQGNGTERIEAELQARF
                     DDVPVLRIDRGSTGHRDALQQHFDDLGEKPGILVGTQMLAKGHDLPNLTLVVVVGIDE
                     GLFSADFRSSEKLAQLLVQVAGRAGRADKPGTVLLQTHHPDHPLLQVLVHGGYHAFAE
                     GELGLRQAAGFPPFAHLALLRAEAKHADPPMQFLHVAKAVLRDMPVDASGPLPAPMPR
                     RAGYQRAQLILSATQRRDLHVALEAMLPVLHAAPDARKVRWSLDVDPVELY"
     gene            3642..5033
                     /locus_tag="PK399_07130"
     CDS             3642..5033
                     /locus_tag="PK399_07130"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HPW12927.1"
                     /translation="MTPAAPAPPSPAAVTAFLRGFDRRARLFAEVQAGDEASARHALA
                     VVARVFASEAGQWPLAEWPRQYWRLLLAAPSMRKPRAVAIAPRLPGIARLAVDARAAV
                     LLHLVAALDDADAAGALGIDTTTYQARIRDALPLDALGQPDVDVWRAWRAAAERELAQ
                     PVEPEAPPASATTPGSPATAHVAASIDADIRHRRRLRWLWVGVALCVLALVATFFLHP
                     RGREVLDHWRAEIKREPLDPAAPPTSRFDAGDLSLHPDRELLSMPAELGVARQLPLLA
                     WLEVAGADPRAADAVRLPVIVAAPDAAATAPAAGDASLARRAQRWDALPAKARAIQRG
                     AWQAWRALEPAERLRLRATATRWGLLASEEQDVLRARFDALPADARHGWWLGPKMGRD
                     WPRVAPLFAFVPEDQRAALLALLRSADAEDIDALERLAQITPPEERDALRLDLLRVPA
                     AERRAWMLTKVQG"
     gene            complement(5045..5335)
                     /locus_tag="PK399_07135"
     CDS             complement(5045..5335)
                     /locus_tag="PK399_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12928.1"
                     /translation="MVVIRAFFVPFAFNHDSKHGSENFEAPNHSALASGFGWIEGLLI
                     LWMPIYLLIMQKRVYAQGWPMTLLKYFVLGTCYTVLLSFAIVASMAIGLVAM"
     gene            complement(5894..7066)
                     /locus_tag="PK399_07140"
     CDS             complement(5894..7066)
                     /locus_tag="PK399_07140"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HPW12929.1"
                     /translation="MNDTAAPITESAPPTPAACRNCGAPLHGPHCYACGQPVKGLVRP
                     LGNLFGDLLDSVFNVDTRILRTIPPLFAKPGFLTNEYFAGRQVRYVTPVRLFFFLCIM
                     AFFLARLSIDEGSNPINVNLNDPVAGEIGAATTEADVITRRDAAVKRLTEARAKIPET
                     PGKAGAEVGFDAQIDQASRNADIRLKQLREAAASGATPPPPMEDTFSIGNNGVWDEKK
                     NPVHLPGAPKFVDGWFNQKIGRAKHNITRIKAEPGQYIDAFVGAIPTALLVLVPLFAL
                     MLKLAFVFKRRLYMEHLVVSLHSHAFLSLDLLLVFACLASQRLVASGSVIDGILDWCV
                     GLLIAWMPIYLLLMQKRVYGQGWPMTLLKYLVLGTCYMTLLGFAISAAAIASLVWM"
     gene            7136..8530
                     /locus_tag="PK399_07145"
     CDS             7136..8530
                     /locus_tag="PK399_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPW12930.1"
                     /translation="MTPEPLQPPRFREEVARTARLAAPLAAGHLSHGLVGFVDAVVAG
                     HHGTATLAAVAVGTALFWLPMMLPMGVLMALPPSVSQLDGAGRRGEIGPLFRQALWLA
                     VALGVLLFGLMTVLPFALAPMGMAADIIPGATDFIHAIRWGIPAFTLYLAMRYLSDGL
                     HWSMPTMVLGFGGLLLLAPLGYVLANGLFGFPELGAVGLGIASALMFWVQAIAFALYL
                     WWSPRFADLQLFSHWHAPRWSVQRDLLRTGLPIGVMVAMEGSLFIVTALLIGRLGEVP
                     VAAHQIAINVASMCFMIPFGVAEATTIRVGHAMGRGDRGGLRRAYFAGLVLVLAMQAT
                     SALVLLSGNQAIAALYTGDTAVVALASSLLLYAALFQFPDGVQVLSAGALRGLKDTRI
                     PMLLAMLAYWGIGMPVGAGLGLALGWGPRGMWLGLTAGLTVAAFLMARRFLRSSLRVP
                     MVSASPSVTVTDGG"
     gene            8604..10496
                     /gene="sppA"
                     /locus_tag="PK399_07150"
     CDS             8604..10496
                     /gene="sppA"
                     /locus_tag="PK399_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011257481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPW12931.1"
                     /translation="MNEPRRRGPIARLAIGLWDTVNFTRRLVFNLLFLALLVLVLGLM
                     FGGGKLAPIGERSTLVIEPEGRLVEQFSCDALSRNIARATNSDDCREVRLRDLLRALD
                     AARTDKRIERVVLRLDDLQPSGFASLREVAAAIARVKAAKKQVIAHGDNYSQGQYLLA
                     AQADEVYLDPMSQGGVMLEGLASYRQYFREGLQDKLGVDMHLFKVGEYKSAAEPYILD
                     AASPESKEADLFWMNDIWQRMLADAGKARGIAPATLGALADALPEEVAAVKGDLAKLA
                     LDKKLVDGLKTREQVEDLLVERGVADDGALGGFRQVALDTYLQHLDGALPQADARPQV
                     AVVVAEGAIAGGELPPGQVGGESTSLLLRDARDDDNVKAVVLRVNSPGGEVFASEQIR
                     REVAALKQAGKPVVVSMGDVAASGGYWISMNADRIYADPSTITGSIGIFGLFPTIDRT
                     LGKIGVRTDGVSTAKYAGAFDITRPLDPGVATTIQSVIEKGYRDFTGKVAQARGRSVE
                     QVDAVARGRVWSGMQAKERGLVDVLGGLDAALADAAARAKLGKPATWQARYIEDSATP
                     LSQWFGGMLQSRAGVSLLQASGVGELVLARQLQAQAPLRFIDNALRNRGNVRVTPLAY
                     CFCTLQ"
     gene            complement(10566..11585)
                     /locus_tag="PK399_07155"
     CDS             complement(10566..11585)
                     /locus_tag="PK399_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPW12932.1"
                     /translation="MAGRVVCFGELLLRLAAPGNELLLQRPHLDVHVGGAEANVAVAL
                     ASFGHGVAMAGTLADNALGRAARDQLRRNGVDTTPLAFAPGRMGLYFYTTGAGQRPSE
                     VLYDRAHSAFAEASPAGYDWPALLAGARLLHLSGITPALGPGTAQAALDGACAARAQG
                     AMVCFDGNFRAKLWAAWDSDPPTVLRGLFAQADIAFADHRDIGLVLGTSFEADDPHAR
                     FANAAGAAFAAFPHLQRIACTTRAQSSVDHHRLGALMATRDGALHACPSMELAGIVDR
                     IGAGDAFAAGVLHGLLSGMDDADSLAFGLASGVLKHYLPGDACTLAVDEVMALVNGER
                     LDVRR"
     gene            11767..14550
                     /locus_tag="PK399_07160"
     CDS             11767..14550
                     /locus_tag="PK399_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007970752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW12933.1"
                     /translation="MRRKTHTRKTPVSILAASIAIALHMVPASALAQDAAQDPQAKTD
                     DKVTELETVQVVSTYRASLEKALDIKRSEKGVVDAIVAQDIGKFPDLNLAESLQRIPG
                     VVIARDAGEGRSITVRGLGPQFTRVRLNGLEAMSSVGSSDGQGGANRGRGFDFNVFAS
                     DLFSQLVVRKTASADVEEGSLGATVDLRTGRPFDYDGFTASANLQGSFNDAADTTTPR
                     FAGLVANSWADGKFGALVSLAYSERESVDEGTGTVRWAGGTTNGGFNPASPYKPALAA
                     DVYSPRFPRYTLQEHDQKRLGVTASLQFKPSDSTLFTLDALYSKIDAVRDEKYIEANG
                     LSASGTTGKRQILVRDGVVENGALVYALMDDVDIRSESRHDEWATTFKQVSLDGEHRF
                     SDTFKLSGKIGTSKSDHSNPVQATVMMDKLNVDNYSYDYRGNPNLPVFDYGFDPTNPA
                     GWNLSVIRLRENYVTNSYDTGQLDFEWALNPSITLRGGIQAKNFAFGSKERRRTATET
                     IVPTFADGTTAVPADMIELASLSGLTGSPNSWTVLNFAKIADTFGIFSDSGTFALGNF
                     AASDQAVGEQDRGAYLMGEFSTDIGEIPFSGNVGVRYVRTNQTATGIATASGTPTPAT
                     VTREYSDTLPSMNLVAEVTPDFLIRFGAAKTMSRPALGSIVPGVTVSVSGSARTVSGG
                     NPFLDPVRAKTADLGFEWYFEEGSMLGLGLFWKDIESFIQTTRETRVYADSGLPASLL
                     NGTTATVNDEFVFTLPVNTPGGKLSGFELNYTQQFSFLPGFLGNFGTQLNYTYVDSKI
                     QYLLSSGAAAQKEDLTGLSKTSWNATLFYEGDRFSGRVSATNRADYLIQVPGTEVGFN
                     SASSGVHGQSGTTILDASVRYKINEHFEVSLEGSNLTNEAQESWVANPALQLPLEYSK
                     TGRQYLLGVRYKF"
BASE COUNT         2289 a         4948 c         5060 g         2351 t
ORIGIN      
        1 gcgaacttgc ggatgccgtc ggcgagtttg tcggtggcca tcgcgtcctc gttgtgctgc
       61 cagcggaacg cggcttcgtc gaggcgtgcg ggcggcgcgg cgcgggtgcc gtcatcgacc
      121 agtgcgggtt cgaccgcggc atcggactgc tccagcgcgg ccagcaggtc gggggaaatc
      181 gtcagccggt cgcagcccgc caaggccagt acttcgccgg tgttgcggaa gctcgcgccc
      241 atcaccacgg tctcgtagcc gtgctgcttg tagtagcgcc agatgcgctg caccgactgc
      301 acgccggggt cgtcctgcgg cgtcgccggc gtggccatgc cgttggccag gtgccagtcg
      361 aggatgcggc cgacgaaggg cgagatcagg aacacgccgg cttccgcgca ggcgaccgcc
      421 tgcgcgaacg agaacagcag ggtgaggttg cactggatgc cgtcgcgctc cagccgttcg
      481 gcggcgcgga tgccttccca cgtgctggcg accttgatca gcaggcgttc ggtgccgatg
      541 ccggcatcgg cgtacagctg caccagcttg tgcgccttgg cgatggtggc ttcggtgtcg
      601 aagctgagcc gtgcatcgac ttcggtggac acccggcccg gcaccagctt gaggatctcg
      661 ccgccgatcg ccaccgccag gcggtcgccg gcgtcgccga ccttgtcgtc gccgcgcgcc
      721 gccgcgaccg cggcctcgat gtgcgaggca tacgcgggca gggccgccgc cttcagcagc
      781 agcgacgggt tggtggtcgc gtcgatcggc ttgaagcggg cgatggcctc gatgtcgccg
      841 gtgtcggcga ccacgacgga atgctggcgg agctgttcaa gctggcgggt catggcgacg
      901 actgcatacg gggtgccctg attgtcgccg atgcgccgcg cgcgaccaat gcgaaggcgt
      961 gatcgccgat gcggaaaacg gcatgcccgc gccgctcagt agagttcgac cgggtccacg
     1021 tccagcgacc agcgcacctt gcgcgcgtcc ggcgcggcat gcagcaccgg cagcatcgcc
     1081 tccagcgcga cgtgcaggtc gcggcgctgc gtggcggaaa ggatcagctg cgcgcgctgg
     1141 tagccggcgc ggcgcggcat gggggcgggc agcgggccgg acgcgtccac gggcatgtcg
     1201 cgaagcacag ccttcgccac gtgcaggaac tgcatcggcg gatcggcgtg cttcgcctcg
     1261 gcgcgcagca gggcaaggtg cgcgaacggc gggaaaccgg cagcctggcg caaccccagt
     1321 tcgccctcgg cgaaggcgtg gtagccgccg tggaccagga cctgcagcag cggatgatca
     1381 gggtggtggg tctgcagcag caccgtgccg ggcttgtcgg cacggccggc gcgacctgcg
     1441 acctgcacca gcaactgcgc gagcttctcg ctgctgcgga aatcggcgga gaacaggcct
     1501 tcgtcgatac cgaccacgac caccagggtc aggttgggca ggtcgtggcc tttcgccagc
     1561 atctgcgtgc cgaccaggat gccgggcttc tcgccgaggt cgtcgaagtg ttgctgcagg
     1621 gcatcgcgat gaccggtgct gccacgatcg atgcgcagca ctggcacatc gtcgaagcgc
     1681 gcctgcaatt cggcctcgat gcgctcggtg ccgttgccct gcggctgcag gcccaggctg
     1741 gcgcaatcgg ggcaggccag cggcgatggc ttgcgatagc cgcagtggtg gcactgcagg
     1801 cgcctgccat gcgcgtgcac cgtcatcggg gtgggcttgt ccggggtgct gcaacgcggg
     1861 cagtgcgcgc tccagccgca gtcatggcaa agcagcaccg gcgcgtagcc gcggcggttg
     1921 cggaacacca gcacctggcc gccggcatcg agtgcgccac ggatcgccag cagcgcgtct
     1981 tcgcccaggc cggcgtgcag cggccgcttg cgcacgtcga gcacgcgcac gtgcggcgcc
     2041 ttggcgttac cggcgcgatg gcgcagccga aggtgcgcgt agcgtcccga ctgtgcgttg
     2101 tgcagcgatt ccagcgacgg tgtggcgctg cccagcaccg ccggcacgcc cagtgcgcgg
     2161 gcgcgcacga tggcgacgtc gcgggcgtgg taacggatcc cgtcgaactg cttgtagctg
     2221 ccgtcgtgct cctcgtcgac gatgacgatg ccggcctccg gcaagggcac gaacaccgcc
     2281 gaacgcgtgc ccacgatcac ccgcgcctgc ccgcgcgcgg cggccagcca gacccgcgcg
     2341 cgttcgttgt cgttcaagcc cgaatgcagc gcgtgcaccg gtacgccgag gcgcttgcgg
     2401 aaacgcgcca gcgcctgcgg ggtcaggccg atctccggta ccagtaccag cgcctgcctg
     2461 ccgcgcgcca ggcagtcggc gatggcgtcc aggtagacct cggtcttgcc gctgccggtg
     2521 acgccatcga gcaggaacgc ggcgaagccg tcggcggcgc ggatggcgtc ggcggcgacg
     2581 cgttgttcgt cgttgaggaa gaaagtgggg tcagagtcgc ctttccgttg gaaaggcgac
     2641 tctgacccca ctttcatttc gctgcgctcc accaatccgc gctcccgcag gccacgcagc
     2701 gacgcacgcc agccgggcat ctcgtcatcg agccaatcct cggcacgcac gtggatcagc
     2761 agcgtcgcaa gcgcctttgg cttgccgctg cgcatcgccg gcagggcggt acggccagcc
     2821 tcggtcagca cccagccgta tgccgccgta tcggccgccg gttcgccacg tcgcagtgcg
     2881 gccggcaggg cggtggccag cacctcgccc agcggcgcat gcaggtaacc cgccagccac
     2941 tgcaacgagg ccagcaactc cccctccagc agcgggtccg gatccggcag ggcgagtgcc
     3001 tggcgcagct ccacgccggg ttcggcatgg ccgtggccga tgacgatccc gcagacctcg
     3061 cgcgagccga acggcacccg cagccgctgc ccgaccgcga ccgttccggc atcgcacccc
     3121 gtgggcggca ggtagtcgaa gagccgcggc agcggcaccg gcagggccac gcgcagcacg
     3181 gggatcgggg ccggcatcgc atcagtctag cggggccgcg ccggcaccct gccgagttgc
     3241 agcgcaaacc tgaggcattg cgcaccaagg tgcgccaagt ccctgttcct gctgggaaag
     3301 cgtgtttatc cacattgcgt gtggataagt ctgtgaatcg actcaggatg accctgcgcg
     3361 agggccgtcg gatgggcctt gtccggccgc tggcgaaaaa accgccattc gatgcattcc
     3421 ttgtaaaatc aataacttag tcgtgaaaca cggttgtcat gattcgttga aaccgcgtga
     3481 aaccggcctg cgtgacgatt tcttgacggc tgtgcacaac tggtttgccc acagcccgcg
     3541 ccattgcttg cggagccgtc catgcggggc gtgtcaagcg gtttttcgga cagcctgcgg
     3601 cgggtcgccc ggctaagatg gggcgtgccc gtgacttgcc catgaccccc gccgcgcccg
     3661 cccctccttc gcccgcggcg gtgaccgcct tcctgcgcgg gttcgaccgc cgcgcgcgcc
     3721 tgttcgccga ggtgcaggcc ggcgacgagg cctcggcccg gcatgccctg gcggtggtgg
     3781 cccgggtgtt cgccagcgag gccgggcaat ggccgctcgc cgaatggccg cgccagtact
     3841 ggcggctgct gctggccgcg ccgtcgatgc gcaagccgcg ggcggtcgcc atcgccccgc
     3901 gcctgccggg tatcgcccgc ttggcggtgg acgcacgcgc agcggtgctg ctgcacctgg
     3961 tcgcggcgct ggacgatgcg gatgcggccg gcgcgctggg catcgacacc acgacctacc
     4021 aggcgcgcat ccgcgatgcc ctgccgctgg atgcgctggg ccagcccgac gtggacgtct
     4081 ggcgcgcctg gcgcgcggca gcggaacgcg aactggcgca gccggtggaa cccgaagcac
     4141 cgccggcgtc ggccacgacg cccggctcgc cggccaccgc gcacgtggct gcttcgatcg
     4201 atgcggacat ccgccatcgc cgtcgcctgc gctggctctg ggtcggcgtg gcgttgtgcg
     4261 tgctggcgct ggtcgccacg ttcttcctgc atccgcgcgg acgcgaggtg ctggaccact
     4321 ggcgcgcgga gatcaagcgc gaaccgctcg acccggccgc accgccaaca tcgcgtttcg
     4381 atgccggcga cctgtccctg catccggacc gcgaattgct gtcgatgccg gccgaactcg
     4441 gcgtcgcccg ccagttgccg ctgctcgcct ggctggaggt cgccggtgcc gatccgcgcg
     4501 cggccgatgc ggtgcgcctg ccggtgatcg tcgcggcccc ggatgccgct gcgaccgcgc
     4561 ctgcagcagg cgacgcgtca ctcgcgcggc gcgcgcagcg ttgggatgcc ttgccggcga
     4621 aggcgcgcgc gatccagcgc ggtgcctggc aggcctggcg ggcgctggag cctgccgagc
     4681 gcctgcgcct gcgcgccacc gccacgcgct gggggttgct ggccagcgaa gaacaggacg
     4741 tgctgcgcgc gcgcttcgac gccctgcccg cggatgcgcg ccatggctgg tggctgggcc
     4801 cgaagatggg ccgcgactgg ccccgggtgg caccgctgtt cgcgttcgtg ccggaggacc
     4861 agcgcgccgc gttgctggcc ctgctgcgaa gcgccgatgc cgaagacatc gatgcgctgg
     4921 aacggttggc gcagatcact ccgccggaag aacgcgatgc gttgcgcctg gatttgctgc
     4981 gcgtgccggc ggcggagcgt cgggcgtgga tgctgacgaa agtgcagggc tgaagcggcc
     5041 tgcactacat cgccaccagc ccgatcgcca tgctcgccac gatcgcgaag ctcagcagca
     5101 ccgtgtagca ggtgcccagc acgaagtact tcaacagggt catcggccag ccctgcgcgt
     5161 agacgcgctt ctgcatgatc agcaggtaga tcggcatcca caggatcagc aggccttcga
     5221 tccagccgaa cccgctggcg agtgcactgt ggttcggcgc ctcgaaattc tcgctgccat
     5281 gcttactatc gtggttgaac gcaaatggga cgaagaaagc tctgattacg accaagcaag
     5341 gtactgattg acttctattt cagaatgcta gaaatagata cccccacagg cccccgctta
     5401 acctcagaaa atcaaggata aagagatgat tgggaaagtc cgtagtttca ttctgattgc
     5461 tattgtcgcg ttagtttcga tgccgagtgc ggcaagtccc ttcttcggaa cgaatgagtc
     5521 gggtgccagt gctagagctg tgaccgtgtg cctgcctatc cttctactta atggccagca
     5581 agttggtgtt gtatgttgtg cttgggttga tggtgacacg gcgtgcgctg tcgggacttt
     5641 ctaaaggcag cgatgtctag aaggccgtgg cagcacggcc ttttttacag gatgtcttcc
     5701 cgtaaatttg gatgagaagg catgcgtcag tgcgtaagcc gctccggtct ttcgtcacca
     5761 cataagccat ccatgccatc gaactcatca acgagatgta tcggaggagc gcgacgcact
     5821 gcgcctcgac gtgctgcgcg tgcccgcttc cgaacggcgg gcgtggacgc tggccagaag
     5881 tgagggctga gcgctacatc cacaccaggc tggcgatggc cgccgcgctg atcgcgaaac
     5941 cgagcagggt catgtagcaa gtgcccagca cgagatattt cagcagcgtc ataggccagc
     6001 cctgtccgta cacgcgcttc tgcatcagca gcaggtagat cggcatccac gcgatcagca
     6061 ggcctacgca ccagtcgagg atgccgtcga tcacgctgcc gcttgccacc agccgctgcg
     6121 aagccaggca ggcgaacacc agcagcaggt cgaggctgag gaaggcgtgg ctgtgtagcg
     6181 acaccaccag gtgctccatg tacagcctgc gcttgaacac gaaggccagt ttcagcatca
     6241 gcgcgaacag cggcaccagc accaacagcg cggtcgggat cgcgccgacg aaggcatcga
     6301 tgtactggcc tggctcggcc ttgatccggg tgatgttgtg cttggcgcgg ccgatcttct
     6361 ggttgaacca gccatcgacg aacttcggag ccccggggag gtgaaccggg ttcttcttct
     6421 cgtcccagac gccgttgttg ccgatgctga aggtgtcttc cattggcggc ggcggcgtgg
     6481 cgccgcttgc ggcggcctcg cggagttgct tcaggcgtat gtcggcattg cgactggcct
     6541 ggtcgatctg ggcgtcgaag ccgacctcgg cgccggcctt gccgggggtt tccggaatct
     6601 tcgcccgcgc ctcggtgagt cgcttcactg cggcgtcgcg ccgggtgatg acatcggcct
     6661 cggtggtagc cgcgcctatt tcgccggcga ccggatcgtt gaggttgacg ttgatcggat
     6721 tggagccctc gtcgatcgaa aggcgggcga ggaagaacgc catgatgcag aggaagaaga
     6781 agagccgcac gggggtgacg taacgcacct ggcggccggc gaagtactcg ttggtgagga
     6841 agcccggctt ggcgaacagc ggcgggatgg tgcgcaggat gcgcgtgtcg acgttgaaca
     6901 cgctgtccag caggtcgccg aacaggttgc ccagtggccg caccagccct ttcaccggtt
     6961 gcccgcaggc atagcaatgc gggccgtgca ggggcgcgcc gcaattgcgg caggcggcgg
     7021 gcgttggggg ggcggattcg gtgatcggcg cggcggtgtc gttcatgggc attccctgct
     7081 tcgccgttaa gatggcagcc cgcgcgtgcc aacgccaccg cgcgtgatcg aatccatgac
     7141 acccgaaccc ctccagccgc cgcgcttccg cgaggaagtg gcccgcaccg cgcgcctggc
     7201 cgccccgttg gcggccgggc atctgtcgca tgggctggtt ggtttcgtcg atgcggtggt
     7261 ggccggccac cacggcaccg ccacgctggc cgcggtggcc gtgggcacgg cgctgttctg
     7321 gttgccgatg atgctgccga tgggcgtgct gatggccttg ccgccgtcgg tctcgcaact
     7381 cgatggggcg ggacgccgcg gcgagatcgg cccgctgttc cgccaggcct tgtggctggc
     7441 ggtcgcgctg ggcgtgctgc tgttcggcct gatgacagtg ctgccgttcg cactggcgcc
     7501 gatgggcatg gccgcggaca tcattcccgg tgccaccgac ttcatccacg cgatccgctg
     7561 gggcatcccg gcgttcaccc tgtacctggc gatgcgttac ctcagcgatg gcctgcactg
     7621 gtcgatgccg accatggtgc tggggttcgg cggcctgctg ttgctggcgc cgctgggcta
     7681 cgtcctggcc aacggcctgt tcggtttccc ggagctcggt gcggtgggcc tcggcatcgc
     7741 ctcggcgctg atgttctggg tgcaggcgat cgcgttcgcg ctgtacctgt ggtggtcgcc
     7801 ccgcttcgcc gacctgcagt tgttctcgca ctggcacgcg ccgcgctgga gcgtgcagcg
     7861 cgacctgctg cgcaccggcc tgccgatcgg ggtgatggtg gcgatggagg gcagcctgtt
     7921 catcgtcacc gcgctgctga tcgggcggct gggtgaagtg ccggtggccg cccaccagat
     7981 cgcgatcaac gtcgcctcga tgtgtttcat gatcccgttc ggcgtggccg aggccaccac
     8041 gatccgggtg gggcacgcga tgggtcgcgg cgatcgcggt gggctgcgcc gtgcgtattt
     8101 cgccgggctg gtgctggtgc tggccatgca ggccacgtcc gcgctggtgc tgttgtccgg
     8161 caaccaggcg atcgccgcgc tgtacaccgg cgataccgcg gtggtcgcgc tggcctcgtc
     8221 gctgctgctg tacgcggcgt tgttccagtt cccggacggc gtccaggtgc tgtcggccgg
     8281 tgccttgcgc ggcctcaagg acacccgcat cccgatgctg ctggcgatgc tggcgtactg
     8341 gggcatcggc atgccggtcg gtgccgggct gggcctcgcg ctgggctggg ggccgcgcgg
     8401 gatgtggctg gggctgaccg ccggcctgac cgtcgccgcg ttcctgatgg cccgccgctt
     8461 cttgcgcagc agcctgcgcg tccccatggt gtccgcctcg ccatcggtca cggtgaccga
     8521 cggcggatga gcgcttcacg cctgcccgct agcatgtcca cgtccacgcg ccgcatcggc
     8581 gttgcctccc ccggagttcc tgcatgaacg aaccgcgccg tcgcggaccc atcgcccgcc
     8641 tcgccatcgg cctgtgggac acggtcaact tcacccgccg gctggtgttc aacctgctgt
     8701 tcctggcact gctggtcctg gtgctggggt tgatgttcgg cggcggcaag ctggcgccta
     8761 tcggcgaacg ctcgaccctg gtgatcgagc cggagggccg cctggtggag cagttcagct
     8821 gcgatgcgct gagccgcaac atcgcgcgtg ccaccaacag cgacgactgc cgcgaagtgc
     8881 gcctgcgcga cctgctgcgc gcactcgacg cggcgcgcac cgacaagcgc atcgagcgcg
     8941 tggtcctgcg cctggacgac ctgcaaccga gcggcttcgc atcgctgcgc gaggtcgccg
     9001 cggccatcgc ccgggtcaag gccgcgaaga agcaggtgat cgcccatggc gacaactaca
     9061 gccagggcca gtacctgctg gccgcgcagg cggacgaggt ctacctggac ccgatgtcgc
     9121 agggcggggt gatgctggaa ggcctggcct cgtaccggca gtacttccgc gagggcctgc
     9181 aggacaagct cggcgttgac atgcacctgt tcaaggtcgg cgaatacaag tccgcggcgg
     9241 aaccctacat cctggatgcg gcatcgcccg aatcgaagga agccgacctg ttctggatga
     9301 acgacatctg gcagcggatg ctggccgatg ccggcaaggc gcgtggcatc gccccggcca
     9361 cgctgggcgc gctggccgat gccttgccgg aggaagtcgc cgcggtgaag ggcgacctgg
     9421 cgaagctggc actggacaag aagctggtcg atggcctgaa gacgcgcgaa caggtcgagg
     9481 acctgctggt cgagcgcggc gtggccgatg acggcgcgct gggtggtttc cgccaggtcg
     9541 cgctggacac gtacctgcag cacctcgacg gtgcgctgcc gcaggccgac gcgcgcccgc
     9601 aggtcgcagt cgtggtggcc gaaggcgcga tcgccggcgg cgagctgcca ccgggccagg
     9661 tcggcggcga atcgacctcg ctgctgctgc gcgacgcgcg cgacgacgac aacgtgaagg
     9721 cggtagtgct gcgggtgaac tcgccgggcg gcgaagtgtt cgcctccgag cagatccgcc
     9781 gcgaagtggc cgcgctgaag caggccggca agccggtggt ggtgtcgatg ggcgatgtcg
     9841 ccgcgtcggg cggttactgg atcagcatga acgcggatcg catctatgcc gatccctcca
     9901 cgatcaccgg ctcgatcggc atcttcgggc tgttcccgac catcgaccgc accctgggca
     9961 agatcggcgt gcgcaccgat ggcgtgtcca ccgcgaaata cgccggtgcc ttcgacatca
    10021 cccgcccgct ggacccgggc gtggccacca ccatccagtc ggtgatcgag aagggctacc
    10081 gcgacttcac cggcaaggtt gcgcaggcgc gtggccgcag cgtcgagcag gtcgatgccg
    10141 tcgcccgcgg ccgcgtctgg agcggcatgc aggcgaagga acgcggcctg gtcgacgtgc
    10201 tcggcggcct ggatgccgcg ctggccgatg ccgccgcgcg ggccaagctg ggcaagcccg
    10261 caacctggca ggcgcgctac atcgaggaca gcgccacgcc gctctcgcag tggttcggcg
    10321 gcatgctgca gagccgtgcc ggcgtgtcgc tgttacaggc gtccggcgtc ggcgaactgg
    10381 tgctggccag gcagttgcag gcgcaggcgc cgttgcgctt catcgacaac gcattgcgca
    10441 accgcggcaa cgtgcgggtc acgccgctgg cctactgctt ctgcacgctg cagtaagtcg
    10501 tccggtttcg agtcaagcga cgcgccgcac ctgcggcgcg tcgtcgtttg cggcggcgga
    10561 aatcctcagc gccggacgtc gaggcgttcg ccgttgacca gcgccatcac ctcatcgaca
    10621 gcgagcgtgc aggcatcgcc cggcaggtaa tgcttgagca cgccgctggc caggccgaac
    10681 gccaggctgt ccgcatcgtc catgcccgac agcaggccgt gcagcacgcc ggcggcgaac
    10741 gcatcgccgg caccgatgcg gtcgacgatg ccggccagtt ccatcgatgg gcaggcatgc
    10801 aatgcgccgt cgcgggtggc catcagcgcg cccaggcgat ggtggtccac gctcgactgc
    10861 gcgcgcgtgg tgcaggcgat ccgttgcagg tgcgggaacg cggcgaaggc agcgcccgcc
    10921 gcattggcga agcgtgcgtg cggatcgtcc gcctcgaatg aggtacccag caccaggccg
    10981 atgtcgcgat ggtcggcgaa ggcgatatcg gcctgcgcga acaggccgcg caggaccgtc
    11041 ggcggatcgc tgtcccatgc cgcccacagc ttggcgcgga agttgccgtc gaagcagacc
    11101 atcgcgccct gcgcgcgtgc cgcgcaggcg ccgtccagcg cggcctgcgc ggtgccgggc
    11161 ccgagcgcgg gggtgatccc cgacaggtgc agcaggcgtg cgcccgcaag cagggctggc
    11221 cagtcgtagc cggccggcga ggcctcggcg aacgcggaat gcgcgcggtc gtacagcacc
    11281 tcgctgggcc gttgcccggc accggtggta tagaaataca ggcccatgcg accgggggcg
    11341 aaggccaatg gcgtggtgtc cacgccgttg cgccgcaact ggtcgcgcgc cgcgcggccc
    11401 agcgcgttgt ccgccaatgt cccggccatc gccaccccat ggccgaagct ggccagcgcc
    11461 accgcgacgt tcgcttcggc gccgcccacg tgcacgtcca ggtgcggccg ctgcagcagc
    11521 aattcgttgc ctggcgcggc cagccgcagc aggagttcgc cgaaacacac cacccgacct
    11581 gccattgccc gctccctgtt gatggatgca ccttaccggc cttggcgcac tgcagcatgc
    11641 cttgtcgtcc ggcttttgct acggttttga ccagcggtgt cattttgccg ctgggaacct
    11701 aagcaactgc gcctgccacg tcaaggcacg catcaaacaa tcgctatgac ccgggagggg
    11761 attcagatgc gccgcaagac ccataccaga aagacaccgg tttccatcct cgccgcatcg
    11821 atcgccattg cgttgcacat ggtgccggcc agcgcgctgg cgcaggacgc cgcgcaggat
    11881 ccgcaggcga agacggacga caaggtcacc gaactggaga ccgtgcaggt ggtcagcacg
    11941 taccgcgcca gcctggagaa ggcgctcgac atcaagcgca gcgagaaggg cgtggtcgat
    12001 gcgatcgtcg cccaggacat cggcaagttc ccggacctca acctggccga gtcgctgcag
    12061 cgcatccccg gcgtggtgat cgcgcgcgat gccggcgaag gccgcagcat caccgtgcgc
    12121 ggcctgggcc cgcagttcac ccgcgtgcgc ctgaacggcc tggaagcgat gagctcggtc
    12181 ggcagcagcg acggccaggg cggcgccaac cgtggccgcg gcttcgactt caacgtgttc
    12241 gcatcggatc tcttctcgca gctggtcgtg cgcaagaccg catcggccga cgtggaggag
    12301 ggctcgctcg gcgcgaccgt ggacctgcgc accggccgtc cgttcgacta cgacggcttc
    12361 accgccagcg ccaacctgca gggcagcttc aacgacgccg ccgacaccac caccccccgc
    12421 ttcgccggcc tggtcgccaa cagctgggcc gacggcaagt tcggcgcgct ggtgtcgctg
    12481 gcgtattccg aacgcgaatc ggtggacgaa ggcaccggca ccgtgcgttg ggcaggcggc
    12541 accaccaatg gcggcttcaa cccggcatcg ccgtacaagc cggcgctcgc cgccgatgtc
    12601 tattcgccgc gcttcccgcg ctacacgctg caggagcacg accagaagcg cctgggcgtc
    12661 accgcctcgc tgcagttcaa gccgagcgac agcaccctgt tcaccctgga cgcgctgtat
    12721 tccaagatcg acgcggtccg cgacgagaag tacatcgagg caaacggact cagtgccagc
    12781 ggcaccaccg gcaagcgcca gatcctggtg cgcgacggcg tggtcgagaa cggcgcgctg
    12841 gtgtatgcgc tgatggacga cgtcgacatc cgttccgaga gccgccacga cgaatgggcg
    12901 accacgttca agcaggtcag cctggacggc gagcatcgtt tcagcgacac cttcaagctg
    12961 agcggcaaga tcggcacctc gaagtcggac cacagcaatc cggtccaggc cacggtgatg
    13021 atggacaagc tcaacgtcga caattacagc tacgactatc gcggcaaccc gaatctcccg
    13081 gtgttcgact atggcttcga cccgaccaac ccggccgggt ggaacctgtc ggtgatccgc
    13141 ctgcgcgaga actacgtcac caacagctat gacaccggcc agctggattt cgaatgggcc
    13201 ttgaacccgt cgatcaccct gcgcggcggc atccaggcca agaatttcgc gttcgggtcc
    13261 aaggaacgcc ggcgcaccgc gaccgagacc atcgtgccga cgttcgcgga cggcaccacc
    13321 gcggtgccgg ccgacatgat cgaactggcg tcgctgagcg gcctgaccgg gtcgccgaac
    13381 agctggaccg tgctgaactt cgccaagatc gccgacacct tcggcatctt cagcgacagc
    13441 ggcaccttcg ccctcggcaa tttcgccgcg agcgaccagg ccgtgggcga gcaggaccgt
    13501 ggcgcctacc tgatgggcga gttctcgacc gacatcggcg agatcccgtt ctccggcaac
    13561 gtgggcgtgc gttacgtccg caccaaccag accgcgaccg gcatcgccac cgccagcggc
    13621 acgccgacac cggccacggt cacccgcgaa tacagcgaca ccctgccatc gatgaacctg
    13681 gtggccgagg tcaccccgga tttcctgatc cgcttcggtg ccgccaagac gatgtcgcgg
    13741 ccggcgctcg gcagcatcgt cccgggcgtc accgtcagcg tcagcggttc cgcgcggacc
    13801 gtctccggcg gcaatccgtt cctcgacccg gtgcgcgcga agaccgccga cctcggcttc
    13861 gagtggtatt tcgaagaggg atcgatgctg ggccttggcc tgttctggaa ggacatcgaa
    13921 agcttcatcc agacgacccg cgagacccgc gtctacgccg acagcggcct gcccgcgagc
    13981 ctgctcaacg gcaccaccgc gacggtcaac gacgaattcg tgttcaccct gccggtcaac
    14041 acgccgggcg gcaagctgag cggcttcgag ctcaactaca cccagcaatt cagcttcctg
    14101 cccgggttcc tcggcaactt cggcacccag ctgaactaca cctacgtcga ctccaagatc
    14161 cagtacctgc tgtccagcgg tgccgccgcg cagaaggaag acctgaccgg cctgtcgaag
    14221 acctcgtgga acgcgaccct gttctacgaa ggcgatcgct tctccgggcg cgtgtcggcg
    14281 accaaccgtg ccgactacct gatccaggtg ccgggtaccg aggtcggctt caacagcgcc
    14341 tccagcgggg tgcatgggca gagcggcacg accatcctgg atgcctcggt ccgctacaag
    14401 atcaacgagc atttcgaggt cagcctggaa ggcagcaacc tgaccaacga agcgcaggaa
    14461 tcctgggtcg ccaacccggc cctgcagttg ccgctggaat acagcaagac cggacgccag
    14521 tacctgctgg gcgtgcgcta caagttctga tcggccaacc cctgcaccgc cggacgctcg
    14581 cgtccggcgg tctttttttg cctgcggcct gcacaccgcg ctggccgcgc ggcgggacga
    14641 tgggtcag
//
LOCUS       DAOOCX010000060        14619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3752_length_14619_cov_9.298201, whole genome shotgun sequence.
ACCESSION   DAOOCX010000060 DAOOCX010000000
VERSION     DAOOCX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14619
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3752_length_14619_cov_9.298201"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1314
                     /gene="sucC"
                     /locus_tag="PK399_07165"
     CDS             148..1314
                     /gene="sucC"
                     /locus_tag="PK399_07165"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPW12934.1"
                     /translation="MNFHEYQAKQLFADYGIAVPAGRVARSPEEAVDAAKAIGGEAWM
                     VKAQIHAGGRGKAGGVKYSKSFDEVREYAKGMLGTKMETYQSAGVALPVDSVLVTQAT
                     DIAQELYLSVMVDRSTQSITFIASADGGVEIEKTAVDNPDAIKTLVVNYVQGLQPYQT
                     RDMGFALGLNAKQVNQLSKIMLGLYKLFNEKDLALVELNPLAILTDGNLAVLDGKINS
                     DDNANFRHPELVEMRDIKQEDETEVLASKYDLNYVTMDGNIGCMVNGAGLAMSTMDVI
                     KLEGGAPANFLDVGGGANKEKVTEAFKLILRSPDVKAIFVNIFGGIVRCDMIAEGIIA
                     AVREVGVKVPVIVRLEGTNVDAGKELLKNSGLAITSADDINDGAKKAVAAAAAA"
     gene            1326..2201
                     /gene="sucD"
                     /locus_tag="PK399_07170"
     CDS             1326..2201
                     /gene="sucD"
                     /locus_tag="PK399_07170"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPW12935.1"
                     /translation="MSVLINKNTKVIVQGFTGSQGTFHAEQMLDYGTQVVGGVTPGKG
                     GTQHLGLPVFNTVAESVKATGADASVIYVPPPFAADAILEAADAGIKVIVCITEGIPV
                     LDMLRVKNTLKGYPDVVLVGPNCPGVITPGECKIGIMPGHIHMPGKVGIVSRSGTLTY
                     EAVKQTTDAGLGQSTCIGIGGDPINGTNFIDALKLFQADPQTQGIILVGEIGGSAEEE
                     AADYISKHVTKPVVGFIAGASAPKGKRMGHAGAIASGGTGTAEGKFAALEAAGVTTVK
                     SPGDLGAAIAKRLAG"
     gene            2302..3348
                     /locus_tag="PK399_07175"
     CDS             2302..3348
                     /locus_tag="PK399_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017171852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HPW12936.1"
                     /translation="MPRTRQPLTPEAASAREGGLVYVGDAEPGLTRRAAGTGFRYLDP
                     AGKPVRDAGTLGRIRALAIPPAYRDVWICRLANGHLQATGRDARGRKQYRYHPRWAEV
                     RDREKFGRIEAFGAALPRLRRQLRRDLALPGFPRRKVAAMVVAVMADTLVRIGNAGYA
                     RDNKSYGLTTLRNRHVQFLRGGRARFRFRGKGGIDHDVVLDDAKLARLLRNCQQLPGQ
                     HLFQYLDDEGHVQPVDSGTVNGYLREAMGEAFTAKDFRTWGGTLAAIRLLAEMPWDEA
                     GASQRAMASMRKAVETDVARQLGNTPAVCRKSYIDPRVYAAWEQGWLARASNGVRGPR
                     QWEALALRLLRRRA"
     gene            3419..3685
                     /locus_tag="PK399_07180"
     CDS             3419..3685
                     /locus_tag="PK399_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014976489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HPW12937.1"
                     /translation="MRIRDDYAGYGKRATNVSINQGLLDEAKALDINLSATLERALEM
                     EVRARKREAWLLENREAIAAYNARIERDGLAGDHVRAFKARNGA"
     gene            3682..3996
                     /locus_tag="PK399_07185"
     CDS             3682..3996
                     /locus_tag="PK399_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HPW12938.1"
                     /translation="MTQFVVYANADAASKRHIPFWLNVQSDLIDATGSRVMVPLITLE
                     RAGKPLARLMPEFAISGKRVVMDTVQITNVPMPMLGKPMGDLSGERLAILAAIDMLIS
                     GI"
     gene            3998..5626
                     /locus_tag="PK399_07190"
     CDS             3998..5626
                     /locus_tag="PK399_07190"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPW12939.1"
                     /translation="MTLRIAMAQFDFPVGDVAGNRDRIARMVTEARDEYQADIVLFPE
                     LALSGYPPEDLLYRPSFLRECQVALDAIAADVQGIVAVVGWPQAAGAVVYNAASVLRD
                     GAVEQTYRKRELPNYAVFDERRYFDVDPDRDDCVFEVDGTQVGLVICEDLWFAEPIAS
                     TVARGAQLVLVPNASPFERDKHAQRDTLLAQRTGETGAAIAYLNVVGGQDALVFDGAS
                     VVADGDGNVHPAAAAFTDQWLVVDFDPATRRFTPVQWMVDGDESRDALAWRAVVRGTR
                     DYFAKNGFKHAWLGLSGGIDSSLVLAIAVDALGAENVTAVRMPSRYTADLSNDLAREQ
                     CDAMGVKLIALPIEKPFQGYLDTLAEVFAGKPVDTTEENLQSRTRGAFLMALSNKFGG
                     LVLTTGNKSEYAVGYATIYGDMCGGYAPIKDLYKTEVFALARWRNTVGDRVVPEAVIE
                     RPPSAELRPDQKDQDSLPPYDVLDAILLRHVDQEQSRQEIVSAGFDAATVDKVLKLVR
                     IGEWKRHQAAPGPKVSRRAFGRERRYPITNGYRE"
     gene            complement(5707..7146)
                     /locus_tag="PK399_07195"
     CDS             complement(5707..7146)
                     /locus_tag="PK399_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPW12940.1"
                     /translation="MTTLRHARLLPLAIAGLLLAGCAAHRETVAETDEAAAVAPREAV
                     AMLAAPPPSPPAPPASAGLAAPPADAGASATLDRVMVSGERARGRVDAKAVAISKPER
                     EEPRQQAQSGTLTAGDYDDLLNPSQYARYAERYLQDRAQPGLPFVDTRTPLKVRVVGK
                     DGQPVAFADVSTTDSHGRALHLPTTANGTAVFFPSLDGLAGRVRLEVATKGTATIERS
                     VDLNRLGRERTITIPLPIAGRAPEALDLVLAIDTTGSMTDELRYLQVELDSIVASLRR
                     IRPGTDIRVGLVVYRDEGDEYVTRRCELTGDLASLRRQLGDQQANGGGDYPEAVDQAF
                     IEANRFDWREDAAKVLLHVADAPPHAERAAAAWEQALALRSRGVHIVPVAASGVADDA
                     QYLMRSMAALTQGRYLFLTDDSGVGLPHDEPDVPCYVVTRLDGLIERVVASLLVGRRV
                     EPQADDILRRTGDYDRGVCKATKQPRIAR"
     gene            complement(7312..8145)
                     /locus_tag="PK399_07200"
     CDS             complement(7312..8145)
                     /locus_tag="PK399_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPW12941.1"
                     /translation="MPARSTLLRPVLLVAFVLAASGCSGIKGMFKDKDKNEGLPVSQL
                     YDKAHGSMEEGRWSTAAETFGRLIAQYPYGPYTEQALMEQAYAEHKAGKYDEAVSTID
                     RFIRTYPTHKNIAYLYYLRGLSNMSRNTVFLSRAFKLDMSTRDLQAPQQAYNDFNIVA
                     TRYPNSRYDADARQRMLFLRNEFARFELNTGLYYLQRGAWVSAADRATYLLETYPQTA
                     YQNDAVALLGEAYTRMGNPTLAADTRRVLQQNDPQHPWLAGNWPKGKGIIARLNPLNG
                     N"
     gene            8251..9246
                     /gene="rluD"
                     /locus_tag="PK399_07205"
     CDS             8251..9246
                     /gene="rluD"
                     /locus_tag="PK399_07205"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HPW12942.1"
                     /translation="MPDPMTDAATPRLAIVPQSAAGRRLDAVLAELFPEYSRSRLSAW
                     IKTGDVMVDGAAPRGRDPVRGGEEITLTAVQETQTHAVAEDIPLDVLYEDEHVFVLDK
                     PAGLVVHPGAGNPAGTLVNALLHRDPTLELLPRAGIVHRLDKDTSGVMVVARTLPAHT
                     SLVAQLSAREVHRQYLAIVVGALVSGGTARTGIDRHPRDRVRMAVREDGKDAVTHFRL
                     RERFRNHTALECRLETGRTHQIRVHMNHLKHPIVGDPLYGGSFKLPKGASDELIAALR
                     GFRRQALHAEVLEFAHPVTGEPIRCSAPVPEDMQRLMKLLREDAAVHAEAERQRR"
     gene            9243..9977
                     /gene="pgeF"
                     /locus_tag="PK399_07210"
     CDS             9243..9977
                     /gene="pgeF"
                     /locus_tag="PK399_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPW12943.1"
                     /translation="MSAWLDADWPAPPGVRALSTMRHGLGVSKPPFDAFNLGARCGDD
                     PEAVAENRRQLDAALVLPSPPRWLRQVHGIGVAREPGFDEPEADAAVTSMPGTVLAIL
                     TADCLPVVFAARDGDEIGAAHAGWRGLGAGVLEATVAAMETPADHLVAWLGPAAGPQA
                     YEIGEEVFDAFVSGDAGAAAAFAPTRPGHWKVDLHALARRRLAAIGVTDVHGGGLCTI
                     SDPFRFFSHRRDQRSGRIATVAWIER"
     gene            9974..10531
                     /locus_tag="PK399_07215"
     CDS             9974..10531
                     /locus_tag="PK399_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HPW12944.1"
                     /translation="MNDLLRPTLFQQALGAAFFNLPAPVRQLHAVRGSARYAGIASVE
                     RGRNPLARLCARIGGLPGSMREVPVTVEFTADARGETWRRDFAGTRMTSRLAFKDGLL
                     RERIGPLQFRHALLANAGAIWWRVAGVRLFGVLPLPARWFRGVRCCERGDEGRYQFTV
                     EARLPLVGHVIRYEGWLAPVDDAAA"
     gene            10518..10943
                     /locus_tag="PK399_07220"
     CDS             10518..10943
                     /locus_tag="PK399_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011052111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HPW12945.1"
                     /translation="MPLPEPAGAVIVFDGVCVLCNGWVAFLLRHDRRGRYRYAAMQSD
                     AGRALLASHGLDPDDPASFLLVERDVGAQPRISTESDAIRRVLAGLGGSWRVAHLVAL
                     VPRPLRDAAYRWLARNRYRWFGRHEACSVPAPEHRQRFL"
     gene            complement(10996..12363)
                     /gene="otsA"
                     /locus_tag="PK399_07225"
     CDS             complement(10996..12363)
                     /gene="otsA"
                     /locus_tag="PK399_07225"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HPW12946.1"
                     /translation="MSRLVVVSNRVAIPGENAPGGLAVALQAALGERGGVWFGWSGKS
                     VRGTSGNVHEQQDGGIRYITVDLSRADVDDYYNGFSNRTLWPLLHFRLDLVDYDRATR
                     EGYRRVNALFADTLAPLLREDDIVWIHDYHLIPLAALLRERGVTCRMGFFLHVPVPSA
                     DLMMAIPDHARLFSTLYAYDLLGFQTSRDNDRFRSYVRLYGGGKLLDDHRVEAPDGRR
                     IRTGAFPISIDTPRIARQAAAAAGKSALRNLHTSLERRLLAIGVDRLDYSKGLPERFQ
                     SFGRYLERHPDQKGSLTYLQIAPVSRGEVNEYQHLRNQLEQIAGHINGQHAEPDWTPL
                     RYVNRNIPHAMLTGYYRMARIGLVTPLRDGMNLVAKEYVAAQDPDDPGVLILSLLAGA
                     ALEMKEALLVNPHDLDGVADAIATAAAMPLAERKERWNAMMARLLEYDIHAWRRAYLE
                     ALEAA"
     gene            complement(12360..14171)
                     /locus_tag="PK399_07230"
     CDS             complement(12360..14171)
                     /locus_tag="PK399_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HPW12947.1"
                     /translation="MNAHDAQRSDAADSGLELGVIGNGSFGALVDPRGRIVWGCLPAF
                     DGDPAFCALLSPRHHEGGDFGIELEDFAHAEQAYIANTAVLRTVLHDTRGGSLELLDF
                     APRWRRHGRFYRPVSIIRRITPLTGNPRIRVLARPLADWGARVPEHSWGSNHVRWLLP
                     AFTLRLTTDVPVRYIREGLPFVLTHRMHLVLGPDEPLARSIDGYVEEALGRTLDYWRE
                     WVRYLSIPLEWQDAVIRSAITLKLCQYEDSGAIIAAMTTSIPEAPGSSRNWDYRYCWL
                     RDAAFVVRALNRLGATRSMEEFLRYIFNLATHDGSLQPLYGIDFGARLEEHEVESLSG
                     YRGMGPVRRGNLAWIQKQHDVYGSVVLASTQLFFDQRLADPGDAGTFSRLELLGEHAF
                     ALFEVPDAGLWEFRGRAEVHTYTAAMCWAACDRLAKIASRLGLEDRVAHWRERADRIH
                     ATTLARAWRADANHFSASFQSDYLDASLLLLADIGFVPSDDPRYVATVEAIGRELRHG
                     DGVFRYVAPDDFGAPETSFTICTFWYIDALAAIGRRDEARALFERVLARRNRLGLLSE
                     DMAFEDGEGWGNFPQTYSHVGLINAAMRLSRSWQEAS"
     gene            complement(14168..>14619)
                     /gene="otsB"
                     /locus_tag="PK399_07235"
     CDS             complement(14168..>14619)
                     /gene="otsB"
                     /locus_tag="PK399_07235"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HPW12948.1"
                     /translation="LCAQAETLAIEHRGAMVENKRAGFALHWRAAPGAANAMRAFADH
                     ALSQLTGYRLQHGDHVVELLPVGGDKGGSIEAFLQEAPFAGRHPVFAGDDLTDESGFR
                     VVNAHGGTSVLVGGRTESAADFGLRDPADVRAWLGMPATVNEGDRGR"
BASE COUNT         2291 a         4848 c         5177 g         2303 t
ORIGIN      
        1 atcgcccgag tatgcaagat gccgaccatg gtcgaacctg cgtgcggcgc tgcaacacgc
       61 taaaatgccg gtccgcccgc gccggccggt ccacggcccc cgcgccgcgt ccatgccggc
      121 atcggcaatc ctcttcccgg gtgacccatg aatttccacg aataccaggc gaaacagctg
      181 tttgccgatt acggcatcgc ggtaccggcg gggcgcgtcg cgcgctcgcc cgaagaggcc
      241 gtcgacgcgg ccaaggcgat cggtggcgag gcctggatgg tcaaggcgca gatccacgcg
      301 ggcggccgcg gcaaggccgg cggcgtgaag tacagcaagt ccttcgacga agtgcgcgaa
      361 tacgccaagg gcatgctcgg cacgaagatg gagacctacc agtcggccgg cgtggcgctg
      421 ccggtggatt cggtgctggt gacccaggcc accgacatcg cccaggagct gtacctgtcg
      481 gtgatggtcg accgctccac ccagtcgatc accttcatcg cctccgccga cggcggcgtg
      541 gagatcgaga agaccgcggt cgacaacccc gacgcgatca agaccctcgt ggtgaactac
      601 gtgcagggcc tgcagccgta ccagacccgc gacatgggct tcgcgctggg cctcaacgcc
      661 aagcaggtca accagctgag caagatcatg ctgggcctgt acaagctgtt caacgagaag
      721 gacctggcgc tggtcgaact gaacccgctg gcgatcctca ccgacggcaa cctggctgtg
      781 ctcgacggca agatcaattc cgacgacaac gccaacttcc gccatcccga gctggtggag
      841 atgcgcgaca tcaagcagga agacgagacc gaagtcctgg ccagcaagta cgacctcaac
      901 tacgtgacca tggacggcaa catcggctgc atggtcaatg gcgccggcct ggcgatgtcc
      961 acgatggacg tgatcaagct ggagggcggc gcgcccgcga acttcctgga tgtcggcggc
     1021 ggcgccaaca aggagaaggt caccgaggcg ttcaagctga tcctgcgctc gccggacgtg
     1081 aaggcgatct tcgtcaacat cttcggcggc atcgtccgct gcgacatgat cgccgagggc
     1141 atcatcgccg cggtcaggga agtgggcgtc aaggtgccgg tcatcgttcg cctggaaggc
     1201 accaatgtgg atgccggcaa ggaactgctg aagaactccg gcctggcgat cacgtcggcc
     1261 gacgacatca acgacggcgc gaagaaagcc gtcgccgccg ccgctgcagc ctgaggcccg
     1321 aacccatgtc cgttttgatc aacaagaaca ccaaggtcat cgtccagggc ttcaccggct
     1381 cgcagggcac cttccacgcc gagcagatgc tcgactacgg cacccaggtc gtcggcggcg
     1441 tgaccccggg caagggcggc acccagcacc ttggcctgcc ggtgttcaac accgtcgccg
     1501 aatcggtgaa ggccaccggc gccgatgcgt cggtgatcta cgtgccgccg ccgttcgcgg
     1561 ccgacgcgat cctggaagcg gccgacgccg gcatcaaggt catcgtctgc atcaccgagg
     1621 gcatcccggt gctcgacatg ctgcgggtca agaacaccct gaagggctac ccggatgtcg
     1681 tgctggtcgg cccgaactgc cccggcgtga tcaccccggg cgaatgcaag atcggcatca
     1741 tgcctggcca catccacatg ccgggcaagg tcggcatcgt gtcgcgcagc ggcacgctga
     1801 cctatgaagc ggtcaagcag accaccgacg ccggcctcgg ccagtcgacc tgcatcggca
     1861 tcggcggcga cccgatcaac ggcaccaact tcatcgacgc gctgaagctg ttccaggccg
     1921 acccgcagac gcagggcatc atcctggtcg gcgagatcgg tggcagcgcg gaggaagaag
     1981 cagccgacta catcagcaag cacgtgacca agccggtggt cggcttcatc gccggtgcgt
     2041 cggcgccgaa gggcaagcgc atgggccatg ccggcgcgat cgcgtcgggc ggcaccggca
     2101 ccgcggaagg caagttcgcc gcgctggaag cggcgggcgt gaccacggtg aagtcgccgg
     2161 gcgacttggg tgcggccatc gccaagcgcc tcgccggctg atatcgctcc atcggttgcg
     2221 atcgacgaag ccgcctttgg gcggcttcgt tgttttcggg ccgtggcgcg gcgttgacgc
     2281 ggccatcggc atcgtgccgg catgccgcgc acacgccaac cgctgacgcc cgaagccgct
     2341 tccgcccgcg aaggcggcct ggtttacgtg ggcgatgcag agcccgggct gacgcgccgc
     2401 gcggcgggga cggggttccg gtacctcgat ccggccggca agccggtgcg cgatgccggc
     2461 accctcggtc gcatccgcgc gctggcgatc ccgcccgctt accgtgatgt ctggatctgc
     2521 aggctcgcca atggccacct gcaggcgacc ggccgcgatg cgcgcggccg caagcagtac
     2581 cgctaccacc cccgctgggc cgaggtccgc gaccgcgaga agttcggccg catcgaggcc
     2641 ttcggcgcgg cgctcccgcg cctgcgccgg caattgcgtc gcgacctcgc cttgcccggc
     2701 ttcccccggc gcaaggtcgc ggcgatggtg gtcgcggtga tggccgacac cctggtccgc
     2761 atcggcaacg ccggctacgc gcgcgacaac aagtcgtacg ggctgaccac gctgcgcaat
     2821 cgccacgtcc agttcctgcg cggtgggcgc gcgcgcttcc ggttccgcgg caaaggcggc
     2881 atcgaccatg acgtggtgct ggacgatgcg aagctggcgc gcctgctgcg taactgccag
     2941 cagctcccgg gccagcacct gttccagtac ctcgatgacg agggccacgt gcagccggtc
     3001 gattccggga cggtcaacgg ctacctgcgc gaggcgatgg gcgaggcctt caccgccaag
     3061 gacttccgca cctggggcgg gaccctggcg gcgatccgcc tgctcgccga gatgccctgg
     3121 gacgaggccg gcgccagcca gcgggcgatg gcatcgatgc gcaaggcggt ggaaaccgac
     3181 gtggcccgcc agctcggcaa cacccccgcg gtctgccgca agtcctacat cgacccgcgc
     3241 gtctacgccg cgtgggagca aggctggctg gcccgcgcat cgaacggcgt gcgcgggccg
     3301 cggcagtggg aggcgctggc gctgcgcctg ctgcggcgaa gagcctgagc caggcgaatg
     3361 cgcttgacga tgaaggagcg aatgcgcatg atcaatgcgc atctgaaagg agaaggctgt
     3421 gcgcatccgg gatgattacg cgggctacgg caagcgggcc accaacgtga gcatcaacca
     3481 ggggctgctg gacgaagcga aggcgctgga catcaacctg tccgccacgc tggagcgtgc
     3541 gctcgagatg gaagtgcgcg cacgcaagcg agaggcatgg ctcctggaaa accgtgaggc
     3601 gattgcggct tacaacgcgc gcatcgagcg cgacggcctg gcgggagacc acgtgcgtgc
     3661 gttcaaggcc cggaacgggg catgacccag ttcgtggtct atgccaatgc ggatgccgca
     3721 tcgaaacggc acatcccgtt ctggctcaac gtgcagagcg acctgatcga tgcgaccggt
     3781 tcgcgggtga tggtgccctt gatcaccctg gagcgcgccg gaaagccgct tgcgagactg
     3841 atgcccgagt tcgcgatttc cggcaagcgc gtggtgatgg ataccgtgca gatcaccaac
     3901 gtgccgatgc cgatgctggg gaaacccatg ggcgacctgt cgggcgagcg gctggccatc
     3961 ctggcggcca tcgacatgct gatttcgggg atatgagatg acgctgcgca tcgcgatggc
     4021 ccaattcgat ttcccggtcg gcgacgtggc cggcaaccgc gaccgcatcg cgaggatggt
     4081 cacggaggcg cgcgacgagt accaagccga catcgtgctg ttccccgagc tcgcgctgag
     4141 cggctacccg ccggaagacc tgctgtaccg gccgtcgttc ctgcgcgaat gccaggtcgc
     4201 gctggatgcc atcgcagcgg acgtgcaggg catcgtggcc gtcgtcggct ggccgcaggc
     4261 ggccggtgcg gtggtgtaca acgccgccag cgtgctgcgc gatggtgccg tcgagcagac
     4321 ctatcgcaag cgcgaactgc ccaactacgc ggtgttcgac gagcgccgct acttcgatgt
     4381 cgatccggat cgcgacgact gcgtattcga ggtcgacggc acccaggtcg ggttagtgat
     4441 ctgcgaggac ctgtggttcg ccgagccgat cgcctcgacg gtggccaggg gcgcgcaact
     4501 ggtgctggtt ccgaatgcct cgccgttcga gcgcgacaag cacgcgcagc gcgacacttt
     4561 gctggcgcag cgcacgggcg aaaccggtgc ggccatcgcg tacctgaatg tcgtcggcgg
     4621 tcaggacgcg cttgtgttcg atggtgcgtc ggtcgtcgcc gacggcgacg gcaacgtgca
     4681 tccggccgcg gcggcgttca ccgaccagtg gctggtggtc gatttcgatc cggccacgcg
     4741 ccgcttcacg ccggtgcaat ggatggtgga tggcgacgaa agccgcgatg ccttggcctg
     4801 gcgcgcggtg gtgcgcggca cccgcgacta cttcgccaag aacggcttca agcatgcgtg
     4861 gttggggctc agcggcggca tcgattcctc gctggtgctg gcgatcgccg tcgatgcatt
     4921 gggggcggaa aacgtcacgg cggtgcgcat gccgtcgcgc tacacggcag acctttcgaa
     4981 tgaccttgca cgggaacagt gcgacgcgat gggcgtgaag ctgatcgcgc tgccgatcga
     5041 gaagcccttc cagggctacc tggacacgtt ggccgaagtg ttcgccggca agccggtcga
     5101 tacgaccgag gaaaacctgc agtcgcgcac gcgtggtgca ttcctgatgg ccttgagcaa
     5161 caagttcggc gggctggtgc tgaccaccgg caacaagagc gaatacgcgg tgggttacgc
     5221 caccatctat ggcgacatgt gcggcggtta cgcgccgatc aaggacctgt acaagaccga
     5281 ggtgttcgcg ctggcgcgct ggcgcaacac ggtcggcgac cgggtggtgc cggaggcggt
     5341 gatcgagcgt ccgccatcgg cggagttgcg gccggaccag aaggaccagg actcgctgcc
     5401 gccctatgac gtgctcgacg cgatcctgct gcgtcacgtc gaccaggagc agtcgcgcca
     5461 ggagatcgtg tcggccggct tcgatgccgc caccgtcgac aaggtgctga agctggtacg
     5521 catcggcgaa tggaagcggc accaggccgc gccgggcccg aaggtgagcc ggcgcgcgtt
     5581 cgggcgcgag cgccgctatc cgatcaccaa cggatatcgc gagtagccgc cggcccatgg
     5641 aaagtggggt cagagtggag ttttcgagaa gcgaaaactc gactctgacc ccactttcag
     5701 tggctgctac cgtgcgatgc gtggctgctt cgttgccttg cacacgccgc gatcgtagtc
     5761 gccggtgcgg cggaggatgt cgtcggcctg tggttcgacg cggcggccga ccagcagcga
     5821 tgccaccacc cgttcgatca ggccgtccag ccgggtgacc acgtagcacg gcacgtccgg
     5881 ctcgtcgtgc ggcaggccga cgccgctgtc gtcggtcagg aacaggtagc ggccttgggt
     5941 gagcgcggcc atcgagcgca tcaggtactg cgcgtcatcg gccacgccgc tggcggccac
     6001 cggcacgata tgcacgcccc gggagcgcaa ggccagggcc tgttcccagg ccgccgcggc
     6061 gcgttccgcg tgcggtggtg cgtcggcgac atgcagcagg accttggccg cgtcctcgcg
     6121 ccagtcgaag cggttggcct cgatgaaggc ctggtcgacc gcttcggggt agtcgccgcc
     6181 accgttggcc tgctggtcgc cgagctgccg gcgcaggctg gcgagatcgc cggtgagctc
     6241 gcagcggcgg gtcacgtact cgtcgccctc gtcgcggtag accaccagtc ccacgcggat
     6301 gtcggtgccc gggcgaatgc gccgcaggga ggcgacgatg ctgtcgagtt ccacctgcag
     6361 gtaacgcaat tcatcggtca tgctgccggt ggtgtcgatg gcgagcacga ggtcgagggc
     6421 ttccggcgcg cgaccggcga tgggcagggg gatggtgatc gtgcgctccc tgccgaggcg
     6481 gttcaaatcc accgagcgct cgatcgtcgc ggtgcccttg gtggccacct cgagtcgcac
     6541 gcgtcccgcc aggccgtcca gcgacgggaa gaacaccgcc gtgccgttgg ccgtggtcgg
     6601 caggtgcagc gcacgcccgt ggctgtcggt cgtcgagaca tcggcgaagg ccaccggctg
     6661 gccatccttg ccgacgacgc gcaccttcag cggcgtgcgt gtatccacga aaggcagtcc
     6721 gggctgcgcg cgatcctgca gatagcgctc ggcatagcgc gcgtactgcg agggattgag
     6781 gagatcatcg tagtcgccgg cagtcagggt gcctgactgc gcctgctggc gaggttcctc
     6841 gcgttcgggc ttgctgatgg cgacggcttt tgcgtcgacg cggccacgcg cgcgctcccc
     6901 gctcaccatc accctgtcga gcgtcgcgct cgcgcccgca tcagccggcg gcgcggcaag
     6961 gccggcagag gcgggtggcg cgggtggtga cgggggcggc gctgcgagca ttgcaaccgc
     7021 ctcgcgcggc gcaaccgcgg cggcttcatc ggtttcggct acggtctcgc ggtgcgcggc
     7081 gcaaccggcc agcagcaggc cggcgatggc cagcgggagc aagcgggcat ggcgaagcgt
     7141 ggtcatcgtt ggcgtcctct gcagtggcgt gggaccggca agccgggcgt gccgagcggg
     7201 aacgcgggag gaccgaaaac ggggttgcgc gccgtcgcgc aaaaaaatgg cgcggggttc
     7261 cggagaaccc cacgccatgc gtgtcagctg cgcctgtccg gcgctggccg ctcagttgcc
     7321 gttgagcgga ttcaggcggg cgatgatgcc cttgcccttc ggccagttgc cggccagcca
     7381 cggatgctgc ggatcgttct gctgcagcac gcgcctggta tcggccgcga gggtgggatt
     7441 gcccatgcgc gtgtaagcct cgccgagcag ggcgacggcg tcgttctggt aggcggtctg
     7501 cgggtaggtt tccagcaggt aggtcgcgcg gtcggcggcg gacacccatg cgccgcgctg
     7561 caggtagtac aggccggtgt tgagctcgaa gcgggcgaac tcgttgcgca ggaagagcat
     7621 gcgctggcgc gcgtctgcgt cgtagcgact gttcggatag cgggtggcga cgatattgaa
     7681 gtcgttgtag gcctgctgcg gcgcctgcag gtcgcgggtg ctcatgtcca gcttgaacgc
     7741 cctggacagg aagaccgtgt tgcggctcat gttgctcagg ccacgcaggt aatagagata
     7801 cgcgatgttc ttgtgggtcg ggtaggtgcg gatgaagcgg tcgatggtcg acaccgcctc
     7861 gtcgtacttg cctgccttgt gttccgcgta ggcctgttcc atcagcgcct gctcggtgta
     7921 ggggccgtac ggatactgtg cgatcaggcg gccgaaggtc tcggctgcgg tgctccagcg
     7981 gccctcttcc atggagccgt gggccttgtc gtacaactgg ctgaccggca agccttcgtt
     8041 cttgtccttg tccttgaaca tgcccttgat gccggagcag ccgctggcgg caaggacaaa
     8101 ggcgacgagc aggacggggc gcagcagggt ggaacgagcg ggcatgggca tcgagtcggc
     8161 gcctggaggc acggaggcct ggagagggtg ggataatacc agcccgtttt gagagtcggg
     8221 ccgttgcccc tgtgcggcgc ctgaaccgtg atgcctgatc ccatgaccga cgccgcaacg
     8281 ccccgcctcg ccatcgtccc ccagtccgcc gccggccggc ggctggatgc ggtgctggcc
     8341 gaattgttcc cggaatactc gcgctcgcgg ctgtcggcgt ggatcaagac cggcgacgtg
     8401 atggtcgatg gtgctgcccc gcgcggccgc gatccggtcc gcggcggaga ggagatcacc
     8461 ctcaccgcgg tgcaggaaac ccagacccat gcggtcgccg aggacattcc gctcgatgtc
     8521 ctgtacgagg acgagcacgt cttcgtcctc gacaagccgg ccggcctggt ggtccatccc
     8581 ggcgccggca atccggccgg gaccctggtc aatgccctgc tgcaccgcga ccccacgctg
     8641 gaactgctgc cgcgcgccgg catcgtccat cgcctggaca aggacaccag cggggtgatg
     8701 gtggtggcgc gcaccttgcc cgcgcacacc tcgctggtcg cccagttgtc cgcgcgcgaa
     8761 gtccatcggc agtacctggc catcgtcgtc ggcgcgctgg tgtccggcgg caccgcccgt
     8821 accggcatcg accgccaccc gcgcgaccgc gtgcgcatgg ccgtgcgcga ggatggcaag
     8881 gacgccgtga cccacttccg cctgcgcgag cgcttccgca accacaccgc gctggaatgc
     8941 cggctcgaga ccggccgcac ccaccagatc cgcgtgcaca tgaaccacct caagcacccg
     9001 atcgtcggcg acccgctgta cggcgggtcg ttcaagttgc ccaagggtgc cagcgacgag
     9061 ctcatcgccg ccctgcgcgg gttcaggcgg caggcgctgc atgcggaggt gctggaattc
     9121 gcgcatccgg tgaccggcga gccgatccgc tgcagcgcac cggtgccgga ggacatgcaa
     9181 cgcctgatga agctgctgcg cgaggatgcc gccgtgcatg cggaagccga gcgccagcgc
     9241 cgatgagcgc gtggctggac gccgactggc ccgcgccgcc cggcgtccgc gcgttgtcca
     9301 ccatgcgcca tggtctgggc gtctccaagc ctccgttcga tgcgttcaac ctcggcgcgc
     9361 gctgcggcga tgatccggag gcggtcgccg aaaatcgccg gcaactggac gccgcgcttg
     9421 tgctgccgtc accaccgcga tggttgcggc aggtgcatgg catcggggtt gcacgcgaac
     9481 cggggttcga tgagcccgag gccgacgccg ccgtcacgtc catgcccggc acggtgctcg
     9541 cgatcctcac cgcagactgc ctgccggtgg tgttcgccgc gcgcgacggc gacgaaatcg
     9601 gcgcggccca tgccggctgg cgtggcctgg gtgcgggcgt gctcgaagcg acggtggcgg
     9661 cgatggaaac gccagcggat catcttgtcg cctggctcgg gccggctgcg ggaccgcagg
     9721 cgtacgaaat cggcgaagag gtcttcgatg cgttcgtgtc gggcgatgcc ggtgcagccg
     9781 cggccttcgc gccgacccgg cccggccact ggaaggtcga cctgcatgcg ctggcgcgtc
     9841 gacgactggc cgccatcggg gtcaccgacg tgcacggcgg tggcctgtgc acgatttccg
     9901 atccgttccg cttcttctcg catcgccgcg accagcgcag tggccgcatc gctaccgtcg
     9961 cgtggataga gcggtgaatg atctgctgcg cccgaccttg ttccagcagg cgttgggcgc
    10021 ggccttcttc aacctgccgg cgccggtgcg gcagttgcac gcagtacgcg gcagcgcgcg
    10081 ttatgccggc atcgccagcg tcgagcgcgg gcgcaatccg ctggcgcggt tgtgcgcgcg
    10141 gatcggcggc ctgccgggca gcatgcgcga ggtcccggtc acggtcgagt tcaccgcgga
    10201 tgcgcgcggc gagacctggc ggcgcgattt cgccggcacc cgcatgacat cgcggttggc
    10261 gttcaaggac ggcctgctgc gcgagcggat cgggccgctg cagttccgcc atgccctgct
    10321 ggcaaatgcc ggtgcgatct ggtggcgggt cgccggcgtg cgcctgttcg gggtgttgcc
    10381 gctgcccgcg cgctggttcc gcggcgtgcg ctgttgcgag cgcggcgatg aaggacgcta
    10441 tcaattcacc gtcgaagcgc gcctgccact ggtcggccac gtgatccgct acgaaggctg
    10501 gctggcgccg gtggacgatg ccgctgcctg agcccgccgg cgccgtcatc gtcttcgatg
    10561 gcgtgtgcgt cctctgcaac gggtgggtgg ctttcctgct gcggcacgat cggcgcggcc
    10621 gctatcgcta cgccgcgatg caatccgatg cgggccgcgc gttgctggcg tcgcacggcc
    10681 tggatcccga cgatcccgct tcgttcctcc tggtcgaacg cgatgtcggc gcgcagccgc
    10741 gcatctccac cgagtccgat gcgatccgtc gcgtgctggc cggactgggc ggcagctggc
    10801 gcgtggcgca cctggtcgca ctggtgccgc ggcccctgcg cgatgccgcc tatcgctggc
    10861 tggcgcgcaa ccgctatcgc tggttcggtc gccacgaagc ctgcagcgtg cccgcgcccg
    10921 agcatcggca gcgctttctc tgacgacagg tgtcggcgat gttcgtcccg gccgaagcgc
    10981 actgcgcggt ggggatcagg ccgcctccag cgcctccagg taggcgcgac gccaggcatg
    11041 gatgtcgtat tccagcagcc gcgccatcat cgcgttccag cgttccttgc gctccgccag
    11101 cggcatcgcg gcggcggtcg cgatggcatc ggccacgcca tccaggtcgt gcggattcac
    11161 cagcagcgct tccttcattt ccagcgccgc gcccgcgagc agggacagga tcagcacgcc
    11221 gggatcgtct ggatcctgcg cggccacgta ttccttggcc accaggttca tgccgtcgcg
    11281 cagcggcgtc accaggccga tccgcgccat ccggtagtaa ccggtgagca tcgcgtgcgg
    11341 gatattgcgg ttgacgtagc gaagcggcgt ccagtccggc tccgcgtgct gcccgttgat
    11401 gtggccggcg atctgttcca gctggttgcg caggtgctgg tactcgttga cctccccgcg
    11461 cgagaccggc gcgatctgca ggtaggtcag gctgcctttc tggtccgggt ggcgctcgag
    11521 gtagcgtccg aagctctgga agcgttccgg cagccccttc gagtagtcga ggcggtccac
    11581 gccgatcgcc agcagccggc gttccaggct ggtgtgcagg ttgcgcagtg cggacttgcc
    11641 cgcggccgcc gcggcctggc gtgcgatgcg cggggtgtcg atgctgatcg ggaacgcacc
    11701 ggtgcggatc cgtcgcccgt ccggtgcctc cacccgatgg tcgtccagga gcttgccgcc
    11761 gccatacagg cggacatagg agcggaagcg gtcgttgtcg cggctggtct ggaagccgag
    11821 caggtcgtag gcgtacagcg tgctgaacag gcgtgcgtgg tccggtatcg ccatcatcag
    11881 gtcggcggag ggcaccggca cgtggaggaa gaagcccatc cggcaggtca cgccgcgttc
    11941 gcgcagcagc gcggccagcg ggatcaagtg gtaatcgtgg atccagacga tgtcgtcctc
    12001 gcgcaacagc ggggccaggg tatcggcgaa cagcgcgttg acgcgtcggt agccctcgcg
    12061 ggtggcgcgg tcgtagtcga ccaggtccag gcggaagtgc agcaacggcc acagcgtgcg
    12121 gttggagaag ccgttgtagt agtcgtccac gtcggcgcgg ctgaggtcca cggtgatgta
    12181 gcggatgccg ccgtcctgct gctcgtgcac gttgccgctg gtgccgcgca cgctcttgcc
    12241 gctccagccg aaccacacgc caccgcgttc ccccagcgcc gcctgcagcg cgacggcgag
    12301 cccgccgggc gcgttttcgc cggggatggc gacgcggttg gagaccacga ccaggcggct
    12361 cacgaggcct cctgccagga acgcgacagg cgcatcgcgg cattgatcag gcccacgtgc
    12421 gagtacgtct gcgggaagtt gccccagccc tcgccatcct cgaaggccat gtcctccgac
    12481 agcagcccga gccggttgcg gcgcgcgagg acgcgttcga acaacgcccg cgcctcgtcc
    12541 ctgcgcccga tcgcggcgag tgcgtcgatg taccagaacg tgcagatggt gaagctggtt
    12601 tccggtgcgc cgaagtcgtc gggggccaca tagcggaaca cgccatcgcc gtggcgcagt
    12661 tcgcggccga tcgcctcgac ggtggccacg tagcgcgggt cgtcgctggg gacgaagccg
    12721 atgtccgcca gcaacagcag ggacgcgtcc aggtagtcgc tctggaatga cgcgctgaag
    12781 tgattggcgt cggcgcgcca cgcgcgcgcc agcgtcgtgg catggatgcg gtcggcgcgc
    12841 tcgcgccagt gcgcgacgcg gtcctccagt cccagccggg aagcgatctt cgccaggcgg
    12901 tcgcaggcgg cccagcacat cgcggccgtg taggtgtgca cctcggcgcg gccgcggaat
    12961 tcccacaggc ccgcgtcggg cacctcgaac agggcgaagg catgctcgcc cagcagttcg
    13021 aggcgggaaa aggtgccggc gtcgccggga tcggccaggc gctggtcgaa gaacaactgg
    13081 gtcgacgcca gcaccacgct gccatagaca tcgtgctgct tctggatcca ggcgaggttg
    13141 ccacgccgca ccgggcccat gccgcggtag cccgacagcg attccacctc gtgttcctcg
    13201 agccgtgccc cgaagtcgat gccgtacagc ggctgcaggc tgccgtcgtg ggtggcgagg
    13261 ttgaagatgt agcggaggaa ttcctccatg ctgcgggtcg cgcccagccg gttgagcgcg
    13321 cgcaccacga acgccgcgtc gcgcagccag cagtagcggt agtcccagtt gcgggaggac
    13381 cccggcgctt ccgggatcga ggtggtcatc gccgcgatga tcgcgccgct gtcctcgtac
    13441 tggcaaagct tcagggtgat cgcgctgcgg atcaccgcgt cctgccattc cagcgggatc
    13501 gacaggtagc gcacccattc gcgccagtag tccagggtgc gacccagcgc ttcctccaca
    13561 tagccgtcga tggagcgcgc cagcggttcg tccgggccca gcaccaggtg catgcggtgg
    13621 gtaagcacga acggcaggcc ttcgcggatg tagcgcaccg gtacgtcggt ggtcaggcgc
    13681 agcgtgaacg ccggcaacag ccaacgcacg tggttgctgc cccagctgtg ctcgggcacg
    13741 cgtgcgcccc agtcggcaag cggccttgcc agcacccgga tgcgcgggtt gccggtcagc
    13801 ggggtgatcc gccggatgat cgacacgggg cgatagaagc ggccatgccg gcgccaacgc
    13861 ggggcgaaat cgaggagctc cagcgatccg ccccgggtgt cgtgcaggac ggtgcgcagc
    13921 acggcggtat tggcgatgta ggcctgttcg gcatgggcga agtcctccag ctcgatgccg
    13981 aagtcaccgc cttcgtggtg gcgcggcgac agcagcgcgc agaacgccgg gtcgccgtcg
    14041 aacgcgggca ggcagcccca gacgatgcga ccacgcggat cgaccagcgc gccgaaactg
    14101 ccgttgccga tgacgccgag ttcgagccct gaatccgccg catcggatcg ttgcgcgtcg
    14161 tgtgcgttca tcggccacgg tctccttcgt tgacagtagc cggcatgccc agccatgcgc
    14221 ggacatcggc cggatcgcgc aggccgaagt ccgcggcgct ctcggttctg ccacccacca
    14281 ggacgctggt gccgccgtgc gcattgacca cgcggaaacc ggattcgtcg gtcaggtcgt
    14341 cgccggcaaa gaccggatgc cggccggcga acggggcctc ctggaggaag gcctcgatcg
    14401 atccgccctt gtcgccgccc accggcagca actccaccac gtggtcgcca tgctgcagac
    14461 gatagccggt cagctgcgac agcgcgtgat cggcgaaggc gcgcatggcg ttggccgctc
    14521 cgggtgcggc acgccagtgc agggcgaatc cggcgcgctt gttctccacc atcgcgccac
    14581 gatgctcgat cgccagcgtc tcggcctgtg cgcacaggc
//
LOCUS       DAOOCX010000061        14246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_3885_length_14246_cov_7.948629, whole genome shotgun sequence.
ACCESSION   DAOOCX010000061 DAOOCX010000000
VERSION     DAOOCX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14246
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3885_length_14246_cov_7.948629"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /gene="pnp"
                     /locus_tag="PK399_07240"
     CDS             complement(<1..1755)
                     /gene="pnp"
                     /locus_tag="PK399_07240"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011259733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPW12949.1"
                     /translation="MAKISKSFQYGNQTVTLETGEIARQAGGAVMVSCDGTVVLCTAV
                     AAKTQREGQDFFPLTVDYQEKFYAGGRIPGGFFKREGRATEKETLTSRLIDRPIRPLF
                     PDGFRNEVQVIATVMSLNPEVDGDILALIGCSAAVALTGAPFDGPIGAAKVGYRNGQY
                     LLNPTVSELKESQLELVVAGTKDAVLMVESEAAELSEDVMLGAVVFGHQQMQVAIDTI
                     NALVAEAGKPKWTWAAPAASAGLVDALKAAIGDRLTQAFAVRDKLERKDAISALKKDV
                     MAALAEQAEANGWKAAELAKEFGELEYQTMRDAVLATKVRIDGRALDTVRPIASKVGI
                     LPRVHGSSLFTRGETQAIVAVTLGTARDGQIIDAVGGEYKEHFLFHYNFPPYSVGEAG
                     RMMGPKRREIGHGRLAKRGVLAVMPTMEAFPYTIRVVSEITESNGSSSMASVCGSSLA
                     LMDAGVPVKAPVAGIAMGLVKEGNDFVVLSDILGDEDHLGDMDFKVAGTSNGISALQM
                     DIKIQGITEEIMKVALAQAKAGRLHILGEMANALTAPRAELSDFAPRLITIKIHPDKI
                     REVIGKGGSVIQAITKETG"
     gene            complement(1915..2175)
                     /gene="rpsO"
                     /locus_tag="PK399_07245"
     CDS             complement(1915..2175)
                     /gene="rpsO"
                     /locus_tag="PK399_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPW12950.1"
                     /translation="MSIDSQKVINEHKRGDNDTGSPEVQVALLTARIEMLTEHFKAHK
                     QDHHSRRGLLMMVNRRRSLLDYLHRKDAERYKALIGKLGLRR"
     gene            complement(2349..3332)
                     /gene="truB"
                     /locus_tag="PK399_07250"
     CDS             complement(2349..3332)
                     /gene="truB"
                     /locus_tag="PK399_07250"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPW12951.1"
                     /translation="MRRPRTVFRGLDGILLLDKPQGLSSNQALQRVRHLFRAEKGGHT
                     GSLDPLATGLLPVCFGEATKIAGGLLGARKAYETVARLGITTDTDDAEGQPLRERPVP
                     ALSIDQIDTALRKLTGRIRQRPPIYSALKRGGEPLYAKARRGEAIEVEERDIEVHAFD
                     LLNAADLLDGQLENGASPTLRLHIECGSGTYVRSLVRDLGELLGCGAHVAELRRLWVD
                     PFREPRMWTLPALEDLREHAGERVLEACLLPVEAGMASWPSVRVSEVQAQRLGRGQGL
                     RGPYRPAGQVAVLDEHGRGLGLGEVDGDGGLRPSRLFRWAVATGKPAGATG"
     gene            complement(3343..3705)
                     /gene="rbfA"
                     /locus_tag="PK399_07255"
     CDS             complement(3343..3705)
                     /gene="rbfA"
                     /locus_tag="PK399_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017116804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPW12952.1"
                     /translation="MAQKSFHRTDRVSAQLRRELGTLVHAAVREHGLCSTSVSDVEVT
                     RDMAHAKVFVTVLLADKALETVKALKDLAPEIRYQLARAMKLRHVPELHFHYDESVDR
                     GERIDTLLRDVRASDDAD"
     gene            complement(3724..6309)
                     /gene="infB"
                     /locus_tag="PK399_07260"
     CDS             complement(3724..6309)
                     /gene="infB"
                     /locus_tag="PK399_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPW12953.1"
                     /translation="MSQQTTIRKLAELVNTPVEKLLEQLAEAGMSFSGPDQVVTSIEK
                     VKLLGFLKRSHGKSDSAADEAAPGKIVLNRSRKQEITVGGGGKTGRGPSTIDVVVRKK
                     VTLNKPGAGGAIDPDSEMAEIHRKLAESRQRNEAEQQALAAKDRQRAEEIAERKRLEE
                     AEAERARLAAAEAAALEESGKPKPGHHGHPKPAPAREADKNAANKGKTAHRGSHAMVA
                     SVEDDDNTARFAGQLHLSASDRARRSSSANTRGKPKQRQRSLDQSRTGTGFQKPVAPM
                     VREIAIGDAITVSDLAQKLALKGGDVVKQLFKMGVMATITQTIDHDTAVLVTEELGHK
                     AVHADAGDAESELLAHVEAVQGDQAQRPPVVTIMGHVDHGKTSLLDYIRTTKVASGEA
                     GGITQHIGAYHVQTPKGTISFLDTPGHAAFTQMRQRGAKLTDIVILVVAADDGVMPQT
                     IEAIKHAKAAKVPLIVAINKIDKSDADPSRVKNDLLAHDVVAEEFGGDTQMVELSAKT
                     GLGVDDLLDAISLQAEVLDLKAVFEGRASGVVIESALDKGRGPVATVLVQQGTLAKGD
                     YLVCGIQYGRVRALFDETGSQVAQAGPSIPVQVLGLSGVPESGDDFVVVADERLAKDV
                     AQQRDAKRRETRLVAAAGNRMEDIMAQMGEGASQQTLNLVVKADVHGSMQALREALTG
                     LSNEQIRINVIGSGVGGITESDAQLAATSKATVIGFNVRADASARRVIEGNGVDLRYF
                     SIIYDVIDQVKQVASNVLGKEIREEIIGIAQVRDVFRSSKFGAVAGCMVIEGVVKRSK
                     PIRVLRDSVVVFEGELESLRRFKENVDEVRVNTECGIGVKAYNDVKPGDQIECFERIE
                     VQRTL"
     gene            complement(6436..7923)
                     /gene="nusA"
                     /locus_tag="PK399_07265"
     CDS             complement(6436..7923)
                     /gene="nusA"
                     /locus_tag="PK399_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020684079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPW12954.1"
                     /translation="MSKELLLVVDAVAAEKGVPESVILEAIEAALASAAKKRYAEQDV
                     LVRVQIDPKDGSYETFRRWEVVADDVVMESPDRQVRLMDAVDESEDAEVGDYIEEQIE
                     NPDFGRIAAQAAKQVIVQRVREAERAQVVDAWKDRVGELVTGVVKRVERGNIYVDLGG
                     NAEAIIAKDKGIPRDVLRTGDRVRGYLFDVRSEPRGPQLFISRAAPEFMMELFKLEVP
                     EVGQGLVSIMACARDPGDRAKIAVLAHDNRTDPIGACIGMRGSRVQAVTNELNGERVD
                     IILWSDNAAQFVINAMAPAEVQSIIVDEDKHSMDLAVAEDKLAQAIGRGGQNVRLASR
                     LTGWQLNVMTADQVQAKSEAEQAASRQLFIDKLEVDEEIAGILVSEGFNSVEEIAYVP
                     VGELLAVEGFDEDIVEELRSRARDALLNDALAVEEGLEEHQPAEDLLSLDGMDEATAY
                     ALAERGIRTREDLAEAATDEIEDVEGMDGERAAALIMEARKHWFE"
     gene            complement(7930..8559)
                     /gene="rimP"
                     /locus_tag="PK399_07270"
     CDS             complement(7930..8559)
                     /gene="rimP"
                     /locus_tag="PK399_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPW12955.1"
                     /translation="MTDKADEIAALLSPTVESLGLELLGIEYLPAAGGSVVRLYIDMP
                     AGDDSRHVGIEDCEAVSREVSAQLDVADPISGNYTLEVSSPGIDRPIFGAAQFARVVG
                     ENAKVVLKLPQDGRRRLQGRVERVEGSTVVFALDAGEFAVDAANIDKARLVPDWIALG
                     MAPAVDDSGRDARPGKQKKKPAGKKPAAKAPTNDKSTNSRAARKPSDAE"
     gene            complement(8731..8807)
                     /locus_tag="PK399_07275"
     tRNA            complement(8731..8807)
                     /locus_tag="PK399_07275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8771..8773),aa:Met,seq:cat)
     gene            complement(8879..10336)
                     /gene="nuoN"
                     /locus_tag="PK399_07280"
     CDS             complement(8879..10336)
                     /gene="nuoN"
                     /locus_tag="PK399_07280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HPW12956.1"
                     /translation="MTTMQATLATPAASEFLPLIPELVLVGSAFALLMLDLFLNEKQR
                     VVTHLLAVASLVLVAALLVAGTGGQGAVLGGMFIRDNMADVLKIGTCVVSAIALLYGW
                     PFLRERGLYKGEIAVLMLFAVVGMMLLVSAGSLVMVYLGLELLALCSYALVAIDRDSP
                     LASEAAMKYFVLGALASGMLLYGMSLVYGASGSIDLATIAANAADAEQRTMLLTGMAF
                     MLAGIAFKFGAAPFHMWVPDVYQGAPTPITLFIGSAPKLAAFAMAMRLLEVGMGPLAE
                     HWRPLLAILAALSLVIGNLVALVQANLKRMLAYSTVSHVGFLFMGLAGGGAIGYAAAM
                     FYALSYAIMATAAFGAIILLSRRGFEADRIEDFKGLNARDPWTAGLVLCVMASLAGVP
                     PFLGFWSKLVVLRAAIEGGMLWLAIVGIVCAVIGAFYYLRVIKAMYFDEPVGEPVVPR
                     TDRTLRVVFGVNALALLAFGVFWSPFMAWCQQAFA"
     gene            complement(10344..11855)
                     /locus_tag="PK399_07285"
     CDS             complement(10344..11855)
                     /locus_tag="PK399_07285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPW12957.1"
                     /translation="MTHWPLLSILIWLPILGGVCTLLAGNARPGAARWIALGFAVATF
                     LCSIPLFMGFDLASPAMQYVEQLAWLPAYDIQYHLGADGISLALIGLTTLTTVLVLIG
                     AWGAIDKRVSQYYAAFMILEGLMIGVFSALDAILFYVFFEGMLIPMFIIIGVWGGPRR
                     VYASVKFFLYTFLGSVFMLVGLVYLYLKGGSWQLADMAALPLTIGEQTWLFFAFLVAF
                     AVKVPMFPVHTWLPDAHVEAPTGGSVILAAIMLKIGGYGFLRFVLPIVPDAGHDYAWL
                     VIALSLVAVVYVGLVALVQEDMKKLIAYSSVSHMGFVTLGTFIAFALVRDVGAEDAAR
                     LGMQGAMVQMISHGFVSGAMFSCVGVLYDRMHSRMIRDYGGVANVMPWFAMFWVLFAM
                     ANSGLPGTSGFVGEFMVILAAFQHSPLVGFAAAATLIIGAGYTLWLTKRVIWGEVGNA
                     HVAELEDLNGREWLVLGVFAAGVLVLGVWPKPLIDLMEPSIAQLAAQLAATKL"
     gene            complement(11887..13992)
                     /gene="nuoL"
                     /locus_tag="PK399_07290"
     CDS             complement(11887..13992)
                     /gene="nuoL"
                     /locus_tag="PK399_07290"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPW12958.1"
                     /translation="MSSALIISKTQLLLIVLLPLLGSLVAGLFGRKIGRQASHSVTIL
                     GVAASCVVSAHVLWQLLQGAAPFNENLYTWFQVGNYSAHVGFMVDKLTAMMMVVVTFV
                     SLLVHVYTIGYMAEDDGYHRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLL
                     IGFWFKKPTAIFANLKAFLVNRVGDFGFLLGIAGVLYWIGTLDYATAFANAGAMRDLT
                     VTVLPGHEWSVPTVVCICLFIGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVTA
                     GIFMVARMSPLFEMSTTALNFILVIGATTAFWTGLIGMVQNDIKRVVAYSTLSQLGYM
                     TVALGVSAYSAAVFHLMTHAFFKALLFLGAGSVIIGMHHEQDMRKMGGLRKYMPITHL
                     TMWIGTLALVGTPFFAGFYSKDTIIEAVAHHAGEHPGSLIAQYANFAVLLGAFVTSFY
                     SFRLLYLTFFGEERFREAHAHDAHGHDAHAHDAHHADAHHDDHGHGHGAHEPHESPWV
                     VTLPLVLLAIPSIAIGFLTVGPMLFGTDMMGHEKQLPFFLGAIDVLKERDVMAALAEE
                     FHGPVAFALHGFKAPAFYLALMGFLLATLLYMVMPEWRKGITRTLAWPIKVLERKYFM
                     DDLWIGGFAGGGVKLGKLSRLFDGKVIDGLVVNGSAGLVGLTAGLVRKLQSGYLYHYA
                     FAMILGLVALLALANHYWL"
     gene            complement(13997..>14246)
                     /gene="nuoK"
                     /locus_tag="PK399_07295"
     CDS             complement(13997..>14246)
                     /gene="nuoK"
                     /locus_tag="PK399_07295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006450557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPW12959.1"
                     /translation="AGIFLNRKNVIMLLMSLELLLLSVNINFVGFSRSLGDPAGQVFV
                     FFILTVAAAEAAIGLAILVTLFRNRRTIDVADIDSLKG"
BASE COUNT         2378 a         4789 c         4718 g         2361 t
ORIGIN      
        1 gccggtttcc ttggtgatcg cctggatcac cgaaccgccc ttgccgatca cttcgcggat
       61 cttgtcgggg tggatcttga tggtgatcag gcgcggcgcg aagtcggaca actcggcgcg
      121 cggcgcggtc agcgcgttgg ccatctcgcc gaggatgtgc aggcggccgg ccttggcctg
      181 cgccagcgcg accttcatga tttcctcggt gatgccttgg atcttgatgt ccatctgcag
      241 cgcggagatg ccgttgctgg tgccggccac cttgaagtcc atgtcgccca ggtgatcctc
      301 gtcgccgagg atgtcggaca gcacgacgaa gtcgttgcct tccttgacca ggcccatcgc
      361 gatgccggcg accggtgcct tgaccggcac gcccgcgtcc atcagcgcca gcgaggaacc
      421 gcagaccgag gccatcgagg acgagccgtt cgactcggtg atctcggaga ccacgcggat
      481 ggtgtacggg aaggcttcca tggtcggcat gacggccagc acgccgcgct tggcaaggcg
      541 tccatggccg atttcgcggc gcttcgggcc catcatgcgg ccggcttcgc ccaccgagta
      601 cggggggaag ttgtagtgga acaggaagtg ttccttgtac tcgccaccga cggcatcgat
      661 gatctggccg tcgcgcgcgg taccgagggt caccgcgacg atcgcctggg tctcgccgcg
      721 ggtgaacagc gaggagccgt ggacgcgcgg caggatgccg accttcgagg cgatcgggcg
      781 cacggtgtcg agcgcacggc cgtcgatgcg gaccttggtg gccagcaccg cgtcgcgcat
      841 ggtctggtat tcgagttcgc cgaattcctt cgccagctcg gcggccttcc agccgttggc
      901 ttcagcctgc tccgccagcg cggccatcac gtccttcttc agtgcggaaa tggcgtcctt
      961 gcgctccagc ttgtcgcgca ccgcgaacgc ctgggtcagg cgatcgccga tggcggcctt
     1021 cagcgcgtcg accaggcccg cgctcgcggc aggcgctgcc caggtccact tcggcttgcc
     1081 ggcttcggcg accagcgcgt tgatggtgtc gatcgcgacc tgcatctgct ggtggccgaa
     1141 caccaccgcg ccgagcatca cgtcttccga cagttccgcg gcttcggatt ccaccatcag
     1201 caccgcgtcc ttggtaccgg cgacgaccag ctcgagctgc gattccttga gctcgctgac
     1261 ggtcgggttg agcaggtact ggccattcct gtaaccgacc ttggcagcgc cgatcgggcc
     1321 atcgaacggc gcgccggtca gggcgactgc cgcagagcag ccgatcaggg caaggatgtc
     1381 gccgtcgact tccgggttca gcgacatcac ggtcgcgatc acctggacct cattgcggaa
     1441 accgtccggg aacagcggac ggatcgggcg atcgatcagg cggctggtca gcgtttcctt
     1501 ctcggtggcg cggccttcgc gcttgaagaa gccacccggg atgcggccgc cggcgtagaa
     1561 cttctcctgg taatccaccg tcagcgggaa gaagtcctgc ccttcgcgct gggtcttggc
     1621 cgccaccgcg gtgcacagca cgacggtgcc gtcgcacgag accatcacgg caccgccggc
     1681 ctggcgggcg atctcgccgg tttcgagggt gacggtctgg ttgccgtact ggaaggactt
     1741 gctgatcttc gccacgtggg aatccttgga tgtcttgggt cgtcgacgca gcggagcccc
     1801 gctccgtgcg ttgcatcgtg aaccgatgcg ggatgcggcc gggccgcgat gaaggtgttg
     1861 cgatggctca aaaacgaacc gcggcgcatt gctgcgccgc ggtgggtgtt tcgatcagcg
     1921 acgcaggccg agtttgccga tcagcgcctt gtaccgctcg gcatccttgc ggtgcaggta
     1981 gtcgagcagg ctgcggcggc ggttgaccat catcagcagg ccacgacggc tgtggtgatc
     2041 ctgcttgtgc gccttgaagt gctcggtgag catctcgatg cgggcggtga gcagggcgac
     2101 ctgcacttcc ggggagccgg tgtcgttgtc gccgcgcttg tgttcgttga tgactttctg
     2161 ggaatcgatg gacatgcttg cctcgtcgtc gcgtggccgg caggaacgtg cgtcccgatg
     2221 ggacgccgca ccgcacggct cgccgtggat ggaatcgtga tggatgggtt ggaacgtgaa
     2281 accgctccgg ggagcagcgg gattatagcc cgcatggcgg ggaccgggca acgcgggatc
     2341 gcaacggctc agcctgtagc gccagcgggc ttgccggtcg ccaccgccca gcggaacagg
     2401 cgcgaaggcc gcaggccgcc gtcgccgtcg acttcgccga gtcccaggcc acgcccgtgc
     2461 tcgtccagta ccgcgacctg cccggccggc cggtacggcc cgcgcaagcc ctggccgcgg
     2521 ccaagccgtt gcgcctggac ctcgctgacc cgcactgatg gccacgaggc catcccggcc
     2581 tcgaccggca acaggcacgc ctcgagcacg cgttcgcccg cgtgctcgcg gaggtcttcc
     2641 aacgcgggca aggtccacat ccgcggctcg cggaacggat ccacccagag ccggcgcaac
     2701 tcggccacgt gggcgccgca ccccagcaat tcgccgaggt cgcgcaccag gctgcgcaca
     2761 taggtcccgg agccgcactc gatgtgcaaa cgcagcgtcg gcgatgcgcc gttctcgagc
     2821 tggccatcca gcaggtcggc ggcattcagc aggtcgaagg cgtggacctc gatgtcgcgt
     2881 tcctcgacct cgatcgcctc gccgcggcgg gccttggcgt acagcggctc gccaccacgc
     2941 ttgagcgcgg aatagatcgg cgggcgctgc cggatgcgcc cggtcagctt ccggagcgcg
     3001 gtgtcgatct ggtcgatgga cagcgccggc accgggcgct cgcgcagggg ttggccctcg
     3061 gcgtcgtcgg tatcggtggt gatgccgagc cgggccacgg tctcgtaggc cttgcgcgcg
     3121 ccgagcaggc caccggcgat cttggtggcc tcgccgaaac agaccggcag caggccggtc
     3181 gcgagcggat cgaggctgcc ggtatggccg cccttctcgg cgcggaacag gtggcgcacg
     3241 cgctgcaggg cctggttgga cgacaggccc tgcggcttgt cgagcagcag gatgccatcc
     3301 agcccgcgga agaccgtgcg cgggcggcgc agcggcgcgg ggtcagtcgg cgtcgtcact
     3361 ggcgcggacg tcgcgcaaca gggtatcgat gcgctcgccg cgatccaccg actcgtcgta
     3421 gtggaaatgc agctccggca catgccgcaa cttcatcgcg cgcgcaagct ggtagcggat
     3481 ctccggtgcc aggtccttga gtgccttgac cgtctccagg gccttgtcgg ccagcaacac
     3541 ggtgacgaag accttggcat gcgccatgtc gcgggtgacc tcgacgtcgg acacgctggt
     3601 cgagcacagg ccatgctcgc gcacggccgc gtgcaccagg gtgcccagct cccggcgcaa
     3661 ttgcgccgag acccggtcgg tacggtggaa tgacttctgc gccatcgggg ttgcgggcac
     3721 gccttacagc gtgcgctgca cctcgatgcg ctcgaagcat tcgatctggt cgcccggctt
     3781 gacgtcgttg tacgccttca cgccaatgcc gcactcggtg ttgacgcgga cttcgtccac
     3841 gttctccttg aagcggcgca gcgattcaag ttcgccctcg aacaccacca cgctgtcgcg
     3901 caggacgcgg atcggcttgc tgcgcttgac cacgccctcg atgaccatgc aaccggcgac
     3961 cgcaccgaac ttggagctgc ggaacacgtc gcggacctgg gcgataccga tgatctcctc
     4021 gcggatttcc ttgcccagca cgttcgacgc gacctgcttc acctggtcga tgacgtcgta
     4081 gatgatcgag aagtaacgca ggtcgacgcc gttgccttcg atcacccggc gcgcggaggc
     4141 gtcggcacgc acgttgaagc cgatcacggt ggccttggag gtggccgcca gctgggcgtc
     4201 ggactcggtg atgccgccca cgccggagcc gatcacgttg atccggatct gctcgttgga
     4261 caggccggtc agggcctcgc gcagggcctg catggaaccg tgcacgtcgg ccttgaccac
     4321 caggttgagg gtctgctgcg aagcgccctc gcccatctgc gccatgatgt cttccatgcg
     4381 gttgcccgcg gcggcgacca ggcgggtctc gcgacgcttg gcgtcgcgct gctgggccac
     4441 gtccttggcc aagcgctcat cggccacgac cacgaagtca tcgccggatt cgggcacgcc
     4501 ggagaggccc aggacctgca ccgggatcga cggaccggcc tgcgccacct gcgaaccggt
     4561 ttcgtcgaac agggcgcgga cgcgcccgta ctggataccg cacaccaggt agtcgccctt
     4621 ggcgagcgtg ccctgctgca ccagcaccgt cgcgaccggg ccgcggccct tgtcgagcgc
     4681 cgattcgatc accacgccac tggcgcggcc ctcgaacacg gccttgagat cgagcacttc
     4741 ggcctgcagc gagatcgcgt cgagcaggtc gtccacgccc aggccggtct tcgccgacag
     4801 ctcgaccatc tgggtgtcgc caccgaactc ttccgccacc acgtcgtgcg ccagcaggtc
     4861 gttcttgacc cgcgacggat cggcgtcgga cttgtcgatc ttgttgatcg ccacgatcag
     4921 cgggaccttc gctgccttcg catgcttgat cgcctcgatc gtctgcggca tcacaccgtc
     4981 gtcggcggcg accaccagga tcacgatgtc ggtcaacttc gcgccgcgct ggcgcatctg
     5041 ggtgaacgcg gcatggcccg gggtgtcgag gaagctgatc gtgcccttcg gcgtctgcac
     5101 gtggtacgcg ccgatgtgct gggtgatgcc gcccgcttcg ccggaggcga ccttggtggt
     5161 gcggatgtaa tccagcaacg aggtcttgcc gtggtcgacg tggcccatga tggtgaccac
     5221 cggcgggcgt tgggcctggt cgccctgcac cgcctcgaca tgcgccagca gttcgctctc
     5281 ggcgtctccg gcgtcggcgt ggactgcctt gtggccgagt tcttcggtca ccagcaccgc
     5341 ggtgtcgtgg tcgatggtct gggtgatggt ggccatcacg cccatcttga acagctgctt
     5401 gaccacgtcg ccgcccttca gcgccagctt ctgggcgagg tcggagacgg tgatcgcatc
     5461 gccgatggcg atctcgcgga ccatcggggc caccggcttc tggaatccgg tgccggtgcg
     5521 cgactggtcc agcgaacgct ggcgctgctt cggcttgccg cgcgtgttgg cgctgctgct
     5581 gcggcgcgca cgatcggagg cggacaggtg caactggccg gcgaagcgcg cggtgttgtc
     5641 gtcgtcttcc acgctcgcga ccatcgcgtg cgaaccgcga tgcgcggtct tgcccttgtt
     5701 ggcggcgttc ttgtcggctt cgcgcgcggg tgccggcttg ggatggccgt ggtggccggg
     5761 tttcggcttg cccgactcct ccagcgccgc ggcttccgcg gcggccaggc gcgcacgctc
     5821 ggcctctgct tcttccaggc gcttgcgctc ggcgatttcc tcggcgcgct ggcgatcctt
     5881 ggccgccaat gcctgctgtt cggcctcgtt gcgctggcgc gactcggcca gcttgcggtg
     5941 gatctcggcc atttcggaat cgggatcgat cgccccgccc gcgcccggct tgttcagggt
     6001 gaccttcttg cgcacgacca cgtcgatcgt gctgggcccg cgaccggtct tgccgccgcc
     6061 gccgacggtg atttcctgct tgcggctgcg gttgagcacg atcttgccgg gcgcggcttc
     6121 gtcggcggcg ctgtcgctct tgccgtgcga acgcttgagg aagcccagca gcttgacttt
     6181 ctcgatgctg gtgacgacct ggtcggggcc gctgaagctc atgccggcct cggccagttg
     6241 ctccagcagt ttctcgaccg gcgtgttcac cagttcggcc agcttgcgga tggtggtttg
     6301 ctgcgacatt cggattccgg tgtctttggc cgcgatggat gcggccggat atcaatggag
     6361 aatcgggcga ttgtaggacg ggcccgagcc cgccagccaa tcaggcgtgg cgggccgcgg
     6421 cccgccctgc ggaactcatt cgaaccagtg cttgcgcgct tccatgatca gggccgcggc
     6481 gcgttcgccg tccatgcctt ccacgtcctc gatctcgtcg gtggcggcct cggccaggtc
     6541 ttcgcgggtg cggatgccgc gttcggccag ggcgtaggcg gtggcctcgt ccatgccgtc
     6601 cagcgacaac aggtcttcgg ccggctggtg ttcctccaac ccttcctcga ccgccagcgc
     6661 gtcgttgagc agcgcgtcgc gggcacgcga gcgcagttcc tcgacgatgt cctcgtcgaa
     6721 gccctcgacc gccagcagtt cgccgaccgg tacgtaggcg atttcctcga cgctgttgaa
     6781 gccttccgac accaggatgc cggcgatttc ctcgtccact tccagcttgt cgatgaacag
     6841 ttggcgggac gcggcctgct cggcctccga cttcgcctgc acctggtcgg cggtcatcac
     6901 gttgagctgc cagccggtca ggcggctggc caggcgcacg ttctggccgc cgcggccgat
     6961 cgcctgcgcc agcttgtcct cggcgaccgc caggtccatc gagtgcttgt cctcgtcgac
     7021 gatgatcgac tgcacttccg ccggcgccat cgcgttgatc acgaactgcg cggcgttgtc
     7081 gctccacagg atgatgtcga cgcgctcgcc gttgagctcg ttggtcaccg cctgcacgcg
     7141 cgaaccgcgc atgccgatgc aggcgccgat cggatcggtg cggttgtcgt gcgccagcac
     7201 cgcgatcttg gcgcggtcgc ccgggtcgcg cgcgcaggcc atgatcgaga ccaggccctg
     7261 gccgacttcc ggcacttcca gcttgaacag ttccatcatg aattccgggg cggcgcggct
     7321 gatgaacagc tgcggcccgc gcggctcgga acgcacgtcg aacaggtagc cgcgcacgcg
     7381 gtcgccggtg cgcagcacgt cgcgcgggat gcccttgtcc ttggcgatga tcgcctcggc
     7441 gttgccgccc aggtcgacat agatgttgcc gcgctcgacg cgcttgacca cgccggtgac
     7501 gagttcgccc acgcggtcct tccaggcatc gacgacctgc gcgcgctcgg cctcgcggac
     7561 gcgctgcacg atcacctgct tggcggcctg cgcggcgatg cgcccgaagt ccgggttctc
     7621 gatctgctcc tcgatgtagt cgccgacctc ggcgtcctcg ctctcgtcga cggcgtccat
     7681 caggcgcacc tggcgatccg gcgattccat caccacgtcg tccgccacca cctcccagcg
     7741 acggaaggtc tcgtagctgc cgtccttggg gtcgatctgc acgcgtacca gcacgtcctg
     7801 ctcggcgtag cgcttcttcg ccgccgacgc cagcgcggct tcgatcgcct cgaggatcac
     7861 cgattcgggc acgcccttct cggcggccac tgcatcgacg acaagcaaca attccttgct
     7921 catttacgct tactccgcat cggacggctt gcgcgccgcc ctgctgttgg tggatttgtc
     7981 gttggttggt gctttcgccg cgggtttctt gcccgccggc ttcttctttt gcttgccggg
     8041 ccgcgcatcg cgcccggaat cgtccacggc gggcgccatg cccagcgcga tccagtccgg
     8101 caccagcctc gccttgtcga tgttggccgc gtcgacggcg aactcgccgg catccagcgc
     8161 gaacaccacg gtcgagccct cgacccgctc gaccctgccc tgcaaccgac gacgcccgtc
     8221 ctgcggcaac ttcagcacga ccttcgcgtt ctcgccgacg acgcgcgcga actgcgcggc
     8281 accgaagatc gggcgatcga tcccgggcga agacacttcc agcgtgtaat tgccgctgat
     8341 cggatcggcg acatccagct gcgccgacac ctcgcggctc accgcctcgc aatcctcgat
     8401 gccgacgtgg cgcgagtcgt cgccggcggg catgtcgatg tacaggcgca cgaccgaacc
     8461 gcccgcggcg ggcaggtatt cgatgcccag cagctccagc cccagcgatt ccaccgtggg
     8521 cgaaagcagc gcggcgattt cgtcagcctt gtctgtcacg ttgcgtccaa tcgaagtgcg
     8581 gaaacagcga atccggaaaa cggaatcccg gaaacgagaa agggcccgat gggccctttt
     8641 ccgatgacgc tggattgcga aacccgggaa cagcggattt cgcggcccta gccctgcaac
     8701 aatcccgtgg aagcgatcgc tgcttgaact tggtagcggg ggcaggattt gaacctgcga
     8761 ccttcgggtt atgagcccga cgagctgcca gactgctcca ccccgcagca gaagcggaat
     8821 tatcgcgatg cgcgcgggat attgcaagcc cgcatcgcgt tttattgtcg tgtaccggtc
     8881 aggcgaacgc ctgctggcac cacgccatga acggactcca gaacacgccg aacgccagca
     8941 gcgccagcgc gttgacgccg aacaccacgc gcagcgtgcg atccgtgcgc ggcaccaccg
     9001 gttcgccgac gggctcgtcg aaatacatcg ccttgatcac gcgcaggtag tagaacgcac
     9061 cgatcaccgc gcaaacgatg ccgacgatcg ccaaccacag catcccgccc tcgatcgccg
     9121 cgcgcagcac gacgagcttg gaccagaaac cgaggaacgg gggtacgccg gccaacgatg
     9181 ccatcacgca cagcaccagg cccgcggtcc acgggtcgcg cgcgttcagg cccttgaaat
     9241 cctcgatgcg gtcggcttcg aagccacgcc gcgacagcag gatgatcgcg ccgaacgccg
     9301 cagtcgccat gatcgcgtag ctcaatgcat agaacatcgc ggcggcgtac ccgatcgcgc
     9361 cgccaccggc caggcccatg aacaggaaac cgacgtggga gacggtcgaa tacgccagca
     9421 tgcgcttgag gttggcctga accagggcca ctaggttgcc gatcaccagc gacagcgcgg
     9481 cgaggatcgc cagcaacggg cgccaatgct cggccagtgg ccccatcccc acctcgagca
     9541 ggcgcatcgc catcgcgaac gcggccagct tgggcgcgga gccgatgaac agggtgatcg
     9601 gggtcggcgc gccctggtag acgtcgggta cccacatgtg gaacggcgca gcaccgaact
     9661 tgaaggcgat gccggcgagc atgaaggcca tgccggtcag cagcatcgtc cgctgttccg
     9721 cgtccgcggc gttcgcggcg atggtggcca ggtcgatgct gccactggcg ccatacacca
     9781 gcgacatgcc gtagagcagc atgcccgagg ccaacgcgcc aaggacgaag tacttcatcg
     9841 ccgcctcgga ggccagcggg ctgtcgcggt cgatcgccac cagcgcgtac gagcacagcg
     9901 ccagtagttc caggcccagg tagaccatca ccaggctgcc ggcggagacc agcagcatca
     9961 tgccgaccac cgcgaacagc atcagcaccg cgatctcgcc cttgtacagg ccgcgttcgc
    10021 gcaggaacgg ccagccatac agcaaggcga tggcggacac cacgcaggtg ccgatcttca
    10081 gcacgtccgc catgttgtcg cggatgaaca tgccgcccag cacggcgccc tgcccgccgg
    10141 tcccggccac cagcaaggcc gcgaccagca ccaggctggc gaccgccagc aggtgggtga
    10201 ccacgcgctg cttctcgttg aggaacaggt cgagcatcag cagcgcgaac gcggacccga
    10261 ccaatacgag ttcggggatc aacggcagga attcgctggc ggcgggcgtg gcgagggtgg
    10321 cttgcatcgt cgtcatcgcc tcttcaaagc ttggtggcgg cgagctgcgc cgccagttgc
    10381 gcgatggagg gttccatcag gtcgatcagc ggcttcggcc acacgcccag caccagcacg
    10441 ccggcggcga acacgcccag caccagccat tcgcgcccat tgaggtcctc gagctcggcg
    10501 acatgcgcat tgccgacttc gccccagatc acccgcttgg tcagccagag cgtgtaaccc
    10561 gcgccgatga tcagggtcgc cgccgcggca aagccgacca gcgggctgtg ctggaacgcc
    10621 gccaggatga ccatgaattc gccgacgaac ccgctggtgc ccggcaagcc ggaattggcc
    10681 atcgcgaaca gcacccagaa catcgcgaac cacggcatca cgttggccac gccgccgtag
    10741 tcgcggatca tccggctgtg catccggtcg tagagcacgc cgacgcagga gaacatcgcg
    10801 ccggacacga agccgtggct gatcatctgc accatcgcgc cctgcatgcc aaggcgtgcg
    10861 gcatcctcgg caccgacatc gcgcaccaat gcgaaggcga tgaaggtgcc cagggtgacg
    10921 aagcccatgt gcgagaccga cgaatacgcg atcagcttct tcatgtcctc ctgcaccagc
    10981 gccaccagtc cgacatagac caccgcgacc agcgacagcg cgatcaccag ccaggcgtaa
    11041 tcgtggccgg catcggggac gatgggcagg acgaaacgca ggaagccgta accgccgatc
    11101 ttgagcatga tcgcggccag gatcaccgag ccgccggtcg gcgcctccac gtgcgcatcc
    11161 ggtagccacg tgtggaccgg gaacatcggc accttgaccg cgaacgccac gaggaaggcg
    11221 aagaacagcc aggtctgctc gccgatggtc agcggcagcg cggccatgtc ggccagctgc
    11281 cagctgccgc ccttgaggta caggtagacc agcccgacca gcatgaacac cgagccgagg
    11341 aaggtgtaca ggaagaactt caccgaggca tacacgcggc gcgggccgcc ccagacaccg
    11401 atgatgatga acatcgggat cagcatgcct tcgaagaaga catagaacag gatcgcgtcg
    11461 agcgcgctga acacgccgat catcaggcct tcgaggatca tgaaggccgc gtagtactgg
    11521 ctcacgcgtt tgtcgatcgc gccccacgca ccgatcagca ccagcaccgt ggtcagcgtg
    11581 gtcaggccga tcagcgccag cgagatgcca tccgcaccga ggtggtactg gatgtcgtag
    11641 gccggcagcc acgcgagctg ctcgacgtac tgcatggccg ggctggccag gtcgaagccc
    11701 atgaacagcg ggatgctgca caggaaggtg gccacggcga agccgagcgc gatccagcgc
    11761 gcagcccccg ggcgggcatt gcccgcaagc agggtgcaca cgccgccgag gatgggcagc
    11821 cagatcagga tgctgaggag aggccagtgg gtcacgcgcg ggtttccatc aagtcgttca
    11881 tccggctcag agccagtagt ggttggccag cgccagcagc gcgaccaggc ccaggatcat
    11941 ggcgaaggcg tagtggtaca ggtagcccga ctgcagcttg cgcaccaggc cggcggtcag
    12001 cccgaccagg ccggcgctgc cgttgaccac gaggccatcg atcaccttgc cgtcgaacag
    12061 gcgcgagagc ttgcccaact tcacgccacc accggcgaaa ccgccgatcc acaggtcgtc
    12121 catgaagtac ttgcgctcca gcaccttgat cggccatgcc agcgtgcggg taatgccctt
    12181 gcgccattcc ggcatgacca tgtacagcag ggtggccagc aggaagccca tcagcgccag
    12241 gtagaacgcc ggcgccttga agccgtgcag cgcgaacgcc accgggccgt ggaactcctc
    12301 ggccagcgcg gccatgacgt cgcgttcctt cagcacgtcg atcgcgccga ggaagaacgg
    12361 cagttgcttc tcgtggccca tcatgtcggt gccgaacagc atcggcccca cggtaaggaa
    12421 gccgatcgcg atcgagggga tcgccagcaa caccagcggc agggtcacca cccatggcga
    12481 ctcgtgcggc tcgtgcgcgc catggccatg gccgtggtcg tcgtgatgcg cgtcggcatg
    12541 gtgggcgtcg tgcgcatgcg catcgtggcc gtgcgcatcg tgcgcatggg cctcgcggaa
    12601 gcgttcttcg ccgaagaacg tgaggtagag caggcggaag ctgtagaagc tggtgacgaa
    12661 cgcgcccagc agcaccgcga aattggcgta ctgcgcgatc aggctgcccg gatgctcgcc
    12721 cgcatggtgg gcgacggcct cgatgatggt gtccttcgag tagaagccgg cgaagaacgg
    12781 cgtgccgacc agcgccagcg tgccgatcca catggtgagg tgggtgatcg gcatgtactt
    12841 gcgcaggccg cccattttcc gcatgtcctg ctcgtggtgc atgccgatga tcacgctgcc
    12901 ggcgccgagg aacagcagcg ccttgaagaa ggcgtgggtc atcaggtgga acaccgccgc
    12961 ggagtaggcg gacacgccca gcgccacggt catgtagccg agctgcgaca gggtcgagta
    13021 cgcgaccacc cgcttgatgt cgttctggac catgccgatc aggccggtcc agaacgcggt
    13081 ggtcgcgccg atcaccagga tgaagttgag cgcggtcgtc gacatctcga acagcggcga
    13141 catgcgcgcg accatgaaga tgccggcggt gaccatcgtc gccgcatgga tcagcgcgga
    13201 gatcggggtc gggccttcca tcgagtccgg cagccagacg tgcaacggca cctgcgccga
    13261 cttgcccatc gcaccgatga acaggcagat gcagaccacg gtcggcaccg accattcatg
    13321 gcccggcaac acggtgacgg tgaggtcgcg catcgcgccg gcgttggcga acgcagtggc
    13381 gtaatcgagc gtgccgatcc agtacagcac gccggcgatg ccgagcagga aaccgaagtc
    13441 gccgacgcgg ttgaccagga aggccttgag gttggcgaag atcgccgtgg gcttcttgaa
    13501 ccagaagccg atcagcaggt aggacaccag gcccaccgct tcccagccga agaacagctg
    13561 caggaagttg ttgctcatca ccagcatcag catgctgaag gtgaacagcg agatgtagct
    13621 gaagaagcga tggtagccat cgtcctcggc catgtagccg atggtgtaga cgtgcaccag
    13681 cagcgacacg aaggtgacca ccaccatcat catcgcggtc agcttgtcga ccatgaagcc
    13741 gacatgcgcc gagtagttgc ccacctggaa ccaggtgtag aggttctcgt tgaacggggc
    13801 cgcgccctgc agcaactgcc agagcacgtg cgcggacacc acgcagctgg cggccacgcc
    13861 gaggatggtc acgctgtgcg atgcctgccg gccgatcttg cggccgaaca aaccggccac
    13921 gaggctgccc agcaagggca gcagcacgat cagcagcagc tgggtcttgg agatgatgag
    13981 tgccgatgac atcgggtcag cccttcagcg aatcgatgtc cgccacgtcg atggtgcgac
    14041 ggttgcggaa cagggtgacc aggatggcga ggccgatcgc ggcttcggcc gcggccacgg
    14101 tcaggatgaa gaacacgaag acctggcccg cgggatcgcc cagcgaccgc gagaacccga
    14161 cgaagttgat gttcaccgag agcagcagca gttccagcga catcagcagc atgatcacgt
    14221 tcttccggtt gaggaagatg ccggcc
//
LOCUS       DAOOCX010000062        13635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_4132_length_13635_cov_6.929013, whole genome shotgun sequence.
ACCESSION   DAOOCX010000062 DAOOCX010000000
VERSION     DAOOCX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 13635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13635
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4132_length_13635_cov_6.929013"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="PK399_07300"
     CDS             <1..1546
                     /locus_tag="PK399_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPW12960.1"
                     /translation="IVAALIAAAVLQFFAGLWIARELGTTLPPPRIWPLLQGALVGGW
                     ILLTFALPPLLALRRVSALAVLRSDLRTPSPPALVLFGLALAGMLAMFWQAAGNPQAA
                     AILLGGLLATAAILAVAGGGLAWLVARAGRSGSGITRYALLNLARRRRLTIAQVVALG
                     TSLMALLLLLLVRTDVWDQWQATVRGDAPNRFVINVQPEQRATFDATLQRLGFSSAGL
                     SPMIRGRYIGPTARERGRPQPEGRGERLMDREFNLSTADTLPEGNTLEAGTFWDAKTA
                     RNQFSVETKFAEQLQWKLGDTLVFDVAGQRIEGRITSLRDVRWESFTPNFFVLASPDL
                     APGLPATYITAVRVPRGDATLATALSREIPNANVIDIDAVTGEIARIGDQAAMVIQVV
                     FWFAFLSGCLVFVAAVAATRDERITELGVLRTLGASTAQLRRSQLVEFGAIGAIAGSI
                     AALAAAGIGSLFAQRVLDLPPTWSPTTLATGAIVGTVAAMLAGWLSMRRQLHATVRDT
                     LGMSTN"
     gene            1631..1852
                     /locus_tag="PK399_07305"
     CDS             1631..1852
                     /locus_tag="PK399_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPW12961.1"
                     /translation="MKLKITAIGNSAGVILPKELLAKLRLDKGDELYAVETPDGIRLT
                     TYDPVFAAQMEVAEKIMRDHRDLLRRLAE"
     gene            1859..2278
                     /locus_tag="PK399_07310"
     CDS             1859..2278
                     /locus_tag="PK399_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017167860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HPW12962.1"
                     /translation="MIVWINRSLALAIHERQMAEHGGGTGIRDEGLLESALARPHRLH
                     AYGDPPPDFADLAASLAYGLARNHPFVDGNKRTAHVCYYAFLAINDVHLEAGLDEMYI
                     QMLTLAEGSLSEADFAAWMRPRLRSKSPNKVHEPRRR"
     gene            complement(2292..3158)
                     /gene="ygiD"
                     /locus_tag="PK399_07315"
     CDS             complement(2292..3158)
                     /gene="ygiD"
                     /locus_tag="PK399_07315"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HPW12963.1"
                     /translation="MSATSLMPAAFLGHGSPMNALERNRFTEAWRAFGASVPRPRAIL
                     AVSAHWHVDGLAVTAMPRPRTIHDFHGFPQALFEVDYPAPGLPELAEEVADTVKPDYV
                     VGLDRDGWGLDHGTWGVLVHAFPDADIPVVQLSLDARKPLDWHVALGAKLAALRKRGV
                     LIVGSGNVVHNLRAIDWQRPDGAYDWARRFDDATRQLMAERPHDIASLATHRDYGMAV
                     PTDEHFLPLLYLAGLASAAGKPAEVLVDGYAYGSLSMTAYTLGRRCPEVAQGGEGAAP
                     LDTTVPPEEANI"
     gene            complement(3293..3826)
                     /locus_tag="PK399_07320"
     CDS             complement(3293..3826)
                     /locus_tag="PK399_07320"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HPW12964.1"
                     /translation="MWRDIVKYGVIAGLVVGGGMYATTWALSGEMPHGWVGMAVGYAT
                     MLVAFSAVFVGIKHQRDVGGGGVIKFWPAFGMGLAISFIAALFYVVAWELVQATIVDD
                     FAGSYGASVIAAEKAKGIDPAALAKLTAEMEAFKTQYADPKFRLPMTFAEIFPVGVLV
                     SLVSALVLRNPRVLPAR"
     gene            complement(3932..4375)
                     /locus_tag="PK399_07325"
     CDS             complement(3932..4375)
                     /locus_tag="PK399_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW12965.1"
                     /translation="MRPRFAMPWIHIVLYAALLAGGTVALQWLDYQRLVRSYPSAIYD
                     GLLATGFLALGLWLGMRLLGRGASPAEPAGEGNPRAAEALGISPRELEVLHEIAAGHS
                     NKEIALRLHVSPNTVKTHVARLLEKLEARRRTDALRKARELGLVP"
     gene            complement(4426..5211)
                     /locus_tag="PK399_07330"
     CDS             complement(4426..5211)
                     /locus_tag="PK399_07330"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPW12966.1"
                     /translation="MRYATERFLYRLSTIQAVRDALWLKGAMLFDLWFDMPHRPTRDA
                     DFLGFGLIDVNLLAGTVREICGVAVDDGMVFDPMSVVVDDIREHARYGGLRVRLKGTL
                     GNARCVVQLDVGYGDAVTPGPEDVAYPTLLDDMPTPRLHVYPRATVVAEKLEAIVSLG
                     MANSRLKDYFDLRALAREGASDERQLAQAIAATFVRRGTALPQGLPLGLTEEFANDAA
                     KVAQWNAFLGKNRLHAPALADVVEELAQFLRTPLRLARGESEA"
     gene            complement(5293..5910)
                     /locus_tag="PK399_07335"
     CDS             complement(5293..5910)
                     /locus_tag="PK399_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HPW12967.1"
                     /translation="MRARHAPPAGRERVLTLAGRPQGVASRELAKVGIHRQVLTRLVA
                     SGEIVRVTRGVYRLMDMPAGEHHGLALASLAMPNGVICLLSALQFHGLGTQLPHEVWI
                     AVDRRTRRPRLQYPPLRVLRFGGAALTEGVERHRLEGREVPIYGIEKTLADCFKYRNK
                     IGLEVALEALREARRERRISLDTLWHFAKVDRVANVMRPYLDAVA"
     gene            complement(5967..6344)
                     /locus_tag="PK399_07340"
     CDS             complement(5967..6344)
                     /locus_tag="PK399_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW12968.1"
                     /translation="MEFVRYVSRDDVLRDFGARTRALRLSRNLSREKVAREAVIPMRS
                     LARFESGERSLDLIDFLRLCLVLGLRDRVPEWVPEFPVLEIARTHRGSQPRRRATKRG
                     EPDPDPREEPEKYWAQVRWILEP"
     gene            complement(6427..6777)
                     /locus_tag="PK399_07345"
     CDS             complement(6427..6777)
                     /locus_tag="PK399_07345"
                     /inference="COORDINATES: protein motif:HMM:NF021483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HPW12969.1"
                     /translation="MKLEKVFAIKWASLYPMYVKKAVAKGRTADEVDQVIRWLTGYTA
                     AGLRKQNEGDADLEAFFGKAPRYNPNAALITGMVCGVRVEDVEHPLMRQIRQLDKLVD
                     ELAKGKAMEKILRR"
     gene            complement(6849..7673)
                     /locus_tag="PK399_07350"
     CDS             complement(6849..7673)
                     /locus_tag="PK399_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPW12970.1"
                     /translation="MTTRPKPAHDDGQRRLAVLIDADNAQASVIEGLLAEVAKYGLAS
                     VKRIYGDFTSQQLGQWKKVLLKHSISPVQQFAYTVGKNATDSSLIIDAMDLMYTGRFD
                     GMCLVTSDSDFTRLAQRLREEGLTVYGFGERKTPDAFVQACDKFVYTEVLRAQEATPT
                     PAKPMQKAAKKVAAKSPRAQGKPAAAAPVAKPAGPLPLPLELIRQAIEEASDDEGWAH
                     LGTMGNYLNKVRPDFDPRLYGQKKLSDLLRQHPRHFKVEERGSAATGGKALYVRAV"
     gene            7801..9675
                     /locus_tag="PK399_07355"
     CDS             7801..9675
                     /locus_tag="PK399_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HPW12971.1"
                     /translation="MIVYHSTRQRFLEDVERSPIEDIIAKAFLERTGRYAPDAEYRAW
                     RNSIMQMADVLADDDMPGSMGVGVELGIPQTAKRIDFVLSGTSGSGDPRVIIVELKQW
                     STSRVSDRDGLVIANRGGHADIEGIHPCYQAWSYAALLEGFNEAVHGGGIGLNPCAYL
                     HNHHRDGIIDAARYAPYIDKAPLFLRGPSERRKLQDFIKQHVKDGDDAQALYRIEHGR
                     IRPSKMLADSLSGMLRGNREFVLVDDQKVVYETCLAKAAMANPTRKQVVIVQGGPGTG
                     KSVVAINLMVALTRQGLLAKYISKNAAPRAVYAQKLKGQRKQAEISMMFGSSGAFIDT
                     EADLFDALIVDEAHRLNEKSGLYGNLGENQIKELIAAAKCTILFCDDDQMVTLADIGH
                     SAEIDRWARHLGADVTHLALASQFRCAGSDGYLAWLDDVLGVRETANPDLVPGTYDFR
                     VVDSPAELDRLIRERNGSNKSRLVAGYCWDWKSKRNRDAYDIVLPASGFSRQWNLGSD
                     GSLWITARDSIEQIGCIHTCQGLELDYVGVIIGPDFAMANGALMTNPAGRSKMDRSIR
                     GWKALTKRDPVGTRERLDRIIRNTYRTLMTRGMKGCYVYCTDPEVADFLRERMRRMGS
                     "
     gene            9715..11640
                     /locus_tag="PK399_07360"
     CDS             9715..11640
                     /locus_tag="PK399_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW12972.1"
                     /translation="MSGHSQFDLLKQRRFLPYFTVQALGAFNDNVFRQAIIGLLGVMA
                     AAGLLDETTRGTYTQLAPAVFILPYFLFSSIAGQFAERTEKSKLIRITTAMEIAIMSL
                     AAVGFLLQDMRVLLFALFATGVQSTLFGPVKYSILPSVLKPEELTGGNGLVEMGTSIA
                     VLLGMLFGGLIFTVAGEHGTQAAAVAVILLAIAGNVLSRMIPAVPASAPDLEVQWNPI
                     PESLAILRLAKKQLAVRNSILGVSWFWFTGTVLTGNLPVYAETHLGGTPTLYVFALAV
                     FSIGVGTGSLLCEKLSARTVEIGLVPLGAIGMSACILHLGFAPAIAAKGLDVGPFLQQ
                     AGAWRVVLDLAGIGLFSGFFVVPLFALIQSRTPKDELSRVIAGMNIQNAVFIVAAALG
                     GVALQQLLGWSIPQLFLALGVASIVVAAWIFSIVPEFLMRFLSWLLVRGLYRLKLHGI
                     EKHVPDEGAALLVCNHVSYMDALILSASIPRPARFVMYYKIFNIPVMRWIFRTAKAIP
                     IAGARENPELMQRAFDEVDAVLAEGELVCIFPEGALTKDGDIAPFKSGVERILQRAAA
                     RGQVVPVVPMALRNMWGSMWSRRANAKEGSMLDRMRVPRRLRAHVDVVAGEPVDGGTA
                     TAELLEAKVRALRGDAA"
     gene            complement(11637..12539)
                     /locus_tag="PK399_07365"
     CDS             complement(11637..12539)
                     /locus_tag="PK399_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HPW12973.1"
                     /translation="MSATLLAVIIALAIGHLAPGIANGLRQFGWYRSWLQWLNAQFPE
                     DSFWRGRWGIALALVVPLLVAGLLQVALDQPLWGFVGLLFDIVLLFWCWGPRDLDLDV
                     AAVLDAPDATSRRAAAARLYPPGVDAPLDGASLVEAVFRNAQRRWFGVLFWFCVLGPF
                     GALLYRLSVLAAEQDADVMPSDTLHGARVWLSLLEWPVTQLIALGLALVGNFDSVLSA
                     WRQDDAFGLDSRLLPNAARASVRSEIAEEVADYTESGISASTALAEVFGELPELRDAM
                     NLAWRVLVLWLAVIALFVVAGWVG"
     gene            complement(12565..13488)
                     /gene="nudC"
                     /locus_tag="PK399_07370"
     CDS             complement(12565..13488)
                     /gene="nudC"
                     /locus_tag="PK399_07370"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HPW12974.1"
                     /translation="MTLTPFPFSFVDGALDRADHLRGDADALQALWPQAQVIVLDTDG
                     RAFADADRGLLALDAAALGNGPGTMTFLGLREGQAWFFADASTLAASAPDRIDLRRAA
                     AQWPAWQATLFAQARALQHWHQRHRHCSACGGRLRFIRAGWLGRCEACNSEHYPRTDQ
                     AVIVAVTDGERLLLGRQASWPPRRWSVVAGFVEPGETLEQTVVREVHEETGVRVHAHT
                     ARYVASQPWPFPGSLMLGFIANAEPDEPVAEEELEAARWFSADEVRVGLANDWASADA
                     EGEGIVLSSPISIARHVIHAWLAEHDARAAS"
     gene            complement(13556..>13635)
                     /locus_tag="PK399_07375"
     CDS             complement(13556..>13635)
                     /locus_tag="PK399_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HPW12975.1"
                     /translation="LTGAQFVIRNPNAKTTCGCGSSFTV"
BASE COUNT         2319 a         4629 c         4439 g         2248 t
ORIGIN      
        1 gatcgtcgcc gcgttgattg cggcggccgt acttcaattc ttcgccggcc tgtggatcgc
       61 gcgcgaactc ggcaccacgc tgccaccgcc gcgcatctgg cccttgctgc aaggcgcgct
      121 ggtcggcggc tggatcctgc tcaccttcgc cctgccgccc ttgctggcgc tgcgtcgggt
      181 cagtgcgctg gcggtgctgc gcagcgacct gcgcacgccg tcgccgccgg cgttggtgct
      241 gttcgggctg gcgctggccg gcatgctggc gatgttctgg caggccgccg gcaatccgca
      301 ggccgccgcg atcctgctcg gtggcctgct ggccacggct gcgatcctgg ccgtcgctgg
      361 cggcgggctg gcctggctgg tggcacgcgc aggccgcagt ggcagcggga tcacccgtta
      421 cgcattgctc aatcttgcgc gtcggcgccg gctcaccatc gcccaggtgg tggcactcgg
      481 caccagcctg atggcgctgc tgctgttgct gctggtgcgc accgatgtgt gggaccagtg
      541 gcaggccacg gtgcgcggcg acgcgcccaa ccgcttcgtc atcaacgtgc agcccgagca
      601 gcgcgcgacc ttcgacgcca cgctgcagcg cctcggcttc agctccgccg gcctctcgcc
      661 gatgatccgc ggacgctaca tcggcccgac cgcacgggag cgcggacgcc cgcagcccga
      721 aggtcgtggc gaacgcctga tggatcgcga attcaacctg tccacggccg ataccttgcc
      781 cgaaggcaac acgctggagg ccggcacgtt ctgggatgcg aagacggcgc gcaaccagtt
      841 ctcggtcgag acgaagttcg ccgaacagct gcaatggaaa ctcggcgaca ccctcgtgtt
      901 cgacgtggcc gggcaacgca tcgagggcag gatcaccagc ctgcgcgacg tgcgctggga
      961 gagcttcacg cccaacttct tcgtgctggc gtcgcccgac ctggcgcccg gcctgccggc
     1021 gacctacatc accgcggtgc gcgtgccgcg cggcgacgcg accctggcca ccgccctgag
     1081 ccgcgagatc cccaacgcca acgtgatcga catcgatgcg gtcaccggcg agatcgcgcg
     1141 catcggcgac caggcggcca tggtgatcca ggtggtgttc tggttcgcct tcctgtccgg
     1201 ctgcctggtg ttcgtggccg cggtcgcggc gacccgcgac gagcgcatca ccgaactcgg
     1261 cgtactgcgc acgctgggtg ccagcaccgc gcaactgcgg cgctcgcagc tggtggaatt
     1321 cggcgcgatc ggcgccatcg ccggcagcat cgccgcgctg gccgcggccg gcatcggcag
     1381 cctgttcgcc cagcgcgtgc tggacctgcc gccgacctgg agcccgacca cgctcgccac
     1441 cggcgccatc gtcggcaccg tggccgcgat gctggccggc tggctcagca tgcgcaggca
     1501 gttgcatgcc acggtgcgcg acaccttggg gatgtccacg aattgagtgc ggcgcacagg
     1561 cgccgccggc ctcttgcaag tcgcggcgca ttctgtataa cgtccgttat aacaacaacg
     1621 gagcgccgcc atgaaactga agatcaccgc catcggcaat tcggccggcg tgatcctgcc
     1681 caaggaactg ctggccaagc tgcgcctgga caagggcgat gagctgtatg cggtggaaac
     1741 cccggatggc attcgcctga ccacctacga ccccgttttt gcggcgcaga tggaagtggc
     1801 cgagaagatc atgcgcgatc accgcgacct gcttcgccgg ctggcggagt aactcgcgat
     1861 gatcgtctgg atcaaccgtt cgctcgcgct cgccatccac gagcggcaaa tggccgagca
     1921 tggtggcggg accggtattc gcgatgaagg ccttctggag tccgcgctgg cacggcccca
     1981 ccggttgcat gcctacggtg acccgccacc ggatttcgcc gatcttgcgg ccagcctcgc
     2041 ctatgggttg gcacgcaatc atccattcgt cgatggcaac aagcggaccg cgcacgtgtg
     2101 ttactacgca ttccttgcca tcaacgatgt ccatctcgag gcaggtttgg acgagatgta
     2161 tatccagatg ctcaccttgg ccgagggctc gttgagcgaa gccgactttg ccgcgtggat
     2221 gcgcccgcgc ctgcgctcga agtcaccgaa caaggtgcat gagcccaggc gacgctaagg
     2281 ctgatccacc gctagatatt cgcctcttcc ggcggcaccg tggtgtccag cggcgcggcg
     2341 ccttcaccgc cttgggcgac ctcggggcaa cgacggccca gcgtgtacgc ggtcatcgac
     2401 agcgatccgt aggcatagcc gtccaccagc acctccgccg gcttgcccgc cgccgaggcc
     2461 aggccggcga gatacaacag cggcaggaaa tgctcgtcgg tgggcaccgc catcccgtag
     2521 tcccgatgcg tcgccagcga ggcgatgtca tgcgggcgct cggccatcag ctggcgcgtg
     2581 gcgtcatcga agcgccgcgc ccagtcgtag gcgccgtccg gccgctgcca gtcgatcgca
     2641 cgcaggttgt gcaccacgtt gccgctgccg acgatgagca cgccacgttt gcgcagcgcc
     2701 gccagcttcg cgcccagcgc aacgtgccag tccaatggct tgcgcgcatc cagcgacagc
     2761 tgcaccaccg ggatgtcggc gtcggggaag gcgtgtacca gcacgcccca ggtaccgtga
     2821 tccaggcccc agccatcgcg gtccaggccg acgacgtaat ccggtttcac tgtatccgcg
     2881 acttcctcgg ccagttcagg caagccgggc gccgggtagt cgacctcgaa caacgcctgc
     2941 ggaaagccgt ggaaatcgtg gatcgtccgc ggccgcggca ttgcggtgac ggcgaggcca
     3001 tccacatgcc agtgcgccga gaccgccagg atcgcccgcg gtcgtggcac cgaggccccg
     3061 aacgcccgcc acgcctcggt gaagcgattg cgttccagcg cgttcatcgg gctgccatgg
     3121 ccgaggaagg cagcgggcat cagggaggtg gcggacatgg caggcactcg caacgcgggg
     3181 gagtcctttc cactgtgggg ccatcacggt cgacaagcga cggcggtggg tggaacggta
     3241 cggtgcggcc gccgggcgga gttcccgctc ttgtgggagg ggcttcaacc ccttaccgcg
     3301 ccggcagcac ccgcggattg cgcaacacca gcgccgacac cagcgaaacc agcacgccga
     3361 ccgggaagat ttcggcgaag gtcatcggca gccggaactt cggatccgcg tactgggtct
     3421 tgaacgcctc catttccgcg gtgagcttgg ccagcgccgc aggatcgatc cccttggcct
     3481 tctccgccgc gatcaccgat gcgccatagc tgccggcgaa gtcgtcgacg atcgtcgcct
     3541 gcaccagctc ccaagccacc acgtagaaca gcgcggcgat gaacgagatc gccaggccca
     3601 tcccgaacgc cggccagaac ttgatcaccc cgccgccgcc gacatcgcgc tggtgcttga
     3661 tgccgacgaa gaccgcgctg aaggccacca gcatggtggc gtagcccacc gccatgccaa
     3721 cccagccgtg cggcatctcc ccggacagcg cccaagtggt ggcgtacatc ccgccgccga
     3781 ccaccagccc cgcgatcacc ccgtacttca cgatgtcacg ccacatgctc cacctccgtt
     3841 gattgaaccg gcgccgattg cgggccaacg cgggccattc gtccatcgcc cgtaagggtg
     3901 atttcgtcgg aatcacccat cgcgatggcg cttacggcac caatccgagc tcgcgcgcct
     3961 tgcgcagggc atcggtgcgc cgccgcgctt ccagcttctc cagcagccgg gccacgtgcg
     4021 tcttcactgt attgggcgag acatgcaggc gcagcgcgat ctccttgttg gaatgcccgg
     4081 cggcgatctc atgcaggact tccagttcac gcggactgat ccccagggcc tccgcggcac
     4141 gcggattgcc ttcgccggcc ggctctgccg gcgaggcacc ccgacccagc aagcgcatcc
     4201 ccagccacag ccccagggcg aggaagccgg tggccagcaa gccgtcgtag atcgccgacg
     4261 ggtagctgcg taccagccgc tggtagtcca gccattgcag ggccacggtg ccgcccgcaa
     4321 gcaacgcggc gtacaggacg atgtggatcc agggcatggc gaagcgcggg cgcatgggcg
     4381 gatgctaccg gtgtggcgct ctttcatccg catcgacgta atcgttcatg cctcgctctc
     4441 gcctctggcc aagcgcagcg gcgttcgcag gaattgcgcg agctcttcca cgacatcggc
     4501 caaggcaggg gcgtgcagac gattcttgcc gaggaaggcg ttccactgcg ccaccttggc
     4561 ggcgtcgttt gcgaactcct cggtcaggcc caacggcaat ccctgcggca gggcggtgcc
     4621 gcgccgcacg aacgtggctg caatggcctg cgccaattgc cgctcatccg atgcaccctc
     4681 ccgcgccaaa gcgcgcaggt cgaagtagtc cttcaggcga ctgttggcca tgccaaggct
     4741 gacgatggcc tcaagtttct ctgcgaccac ggtggcgcgc ggatagacat gcaggcgtgg
     4801 ggtaggcatg tcatccagca aggtcggata ggccacgtcc tccggtcccg gggtgaccgc
     4861 atcgccgtag cccacgtcca gttggaccac gcatcgcgca ttgcccaaag tccccttgag
     4921 gcgcacgcgc aaaccaccgt aacgcgcatg ctcgcggata tcgtccacca cgaccgacat
     4981 cgggtcgaac accatgccat cgtccaccgc cacaccacag atttcccgca cggtgcccgc
     5041 cagcaggttg acgtcgatca gcccgaaacc gaggaaatcc gcatcccgcg tgggccgatg
     5101 cggcatgtcg aaccagagat cgaacagcat cgcgcccttg agccacaagg catcccgtac
     5161 ggcctggatc gtggagagtc gatacaggaa gcgctccgtg gcatagcgca ccacgatcag
     5221 gttgaagtcc tcacccccgc tcccgcgcca gattcagcaa gcgggcatgg atcgatgcag
     5281 ccctcgccga cgtcatgcga cagcgtccag atacgggcgc atgacgttag cgacgcgatc
     5341 caccttggcg aagtgccaca gcgtgtcaag gctgatccgg cgttcccggc gcgcttcgcg
     5401 cagcgcttcc agcgccacct ccagcccaat cttgttgcgg tacttgaaac agtccgcgag
     5461 cgtcttctcg atcccgtaga tcggaacctc tcgcccttct aggcgatggc gctccacgcc
     5521 ttcggtcagc gccgccccgc cgaagcgcag cacccgtaac ggcgggtact gcaggcgcgg
     5581 tcggcgcgtg cgtcgatcca cggcgatcca gacctcgtgc ggcagctgcg tgcccagccc
     5641 gtggaactgc agtgcgctaa gcaggcagat cacgccgttc ggcatcgcca gcgacgccag
     5701 cgccagccca tggtgttccc ccgccggcat gtccattagc cgatacacac ctcgcgttac
     5761 ccgcacgatc tcgccgctgg ccaccagtcg ggtcagcact tggcgatgaa tgccgacctt
     5821 cgcgagctca cgcgaggcaa ccccttgcgg gcgacctgcc aaggtcagga ctcgttctcg
     5881 gccggcagga ggggcgtgac gcgctctcat gtggccatta gcagacagtt gatgacaagt
     5941 gtcaacactt agccacatca aaatcctcag ggttccaaga tccagcgcac ctgcgcccaa
     6001 tacttttcag gctcttcacg tggatccgga tcgggttccc cacgcttggt cgcccgcctt
     6061 ctgggttgcg agccacggtg tgtacgagca atctccagca caggaaactc aggcacccat
     6121 tccggaacgc gatcgcgcaa acccaacaca aggcacaggc gcaagaagtc gatcaggtcc
     6181 aacgaccgct cgccgctctc gaagcgggcc aggctacgca tcggaatgac agcctcccgg
     6241 gcgactttct cgcggctcag gttgcgtgac agcctcaatg ctcgggtgcg cgcgccgaaa
     6301 tcgcgcagca cgtcgtcgcg actgacataa cggacaaact ccatgtcact tgctccatgg
     6361 acggaatagc gagcgaagcg ccggactggc acctcgcaga actccttgcg tggaaccgcg
     6421 gggctctcac ctccgcagga tcttctccat cgccttgccc ttggccagtt cgtccaccag
     6481 cttgtccagc tgccggatct ggcgcatcag cgggtgctcg acgtcctcca cccgcacccc
     6541 gcagaccatg ccggtgatca gcgccgcgtt cgggttgtac ctgggtgcct tgccgaagaa
     6601 cgcctcgaga tcggcatcac cctcgttctg cttgcgaagc cctgccgccg tgtagccggt
     6661 cagccagcgg atgacctggt ccacttcatc cgcggtgcgg cccttggcca cggctttctt
     6721 cacgtacatc gggtacaggc tggcccattt aatggcgaag actttttcga gcttcatcgc
     6781 atccacctca accctgcttc tgtgggaggg gcttcagccc cgacggagtt cgccgaggtc
     6841 tttgcccgct acaccgccct gacgtacaac gccttgccac ccgtcgccgc ggatccgcgc
     6901 tcctccacct tgaagtgccg cggatgctgg cgcagcaggt cgctgagctt cttctgcccg
     6961 tacaggcgcg ggtcgaagtc gggcctgacc ttgttgaggt agttgcccat cgtgcccagg
     7021 tgcgcccagc cctcgtcgtc ggacgcctcc tcgatcgcct ggcggatcag ctccagcggc
     7081 aacggcagcg gccccgcggg tttcgccacg ggtgccgcgg cagcgggctt gccctgcgcc
     7141 cgcggggact tggccgcgac cttcttcgcc gctttctgca tcggcttggc gggcgttggc
     7201 gtcgcttcct gcgcgcgcag cacctcggtg tagacgaact tgtcgcaggc ctgcacgaag
     7261 gcatccggcg tcttgcgctc gccgaaaccg tacacggtca gcccctcctc gcgcaggcgc
     7321 tgcgccaggc gggtgaaatc gctgtcgctg gtgaccaggc acatgccatc gaaacggccg
     7381 gtgtacatca ggtccatcgc atcgatgatc agcgaactgt cggtggcgtt cttgcccacc
     7441 gtgtaggcga actgctgcac cgggctgatc gaatgcttga gcagcacctt cttccactgc
     7501 cccagctgct gcgaggtgaa gtcgccatag atccgcttca cgctggccag cccgtacttg
     7561 gccacctccg ccagcagacc ctcgatcacc gacgcctgcg cgttgtcggc atcgatcagc
     7621 accgccagcc ggcgctggcc gtcgtcgtgc gctggcttgg ggcgggtggt catggcgggc
     7681 tccggggcgt ggcggcgtca ggggcgattg aaccacgatg cacgcggcgt cgcgccccct
     7741 cgcttgcagg ctgccacgac tgcacgtaca caggtggctg accacgagga tgccgtacat
     7801 gtgatcgtct accactcgac ccgccagcgc tttctcgaag acgtggaacg cagcccgatc
     7861 gaggacatca tcgcgaaggc attcctcgaa cgcacgggaa ggtacgcgcc ggatgcagag
     7921 taccgcgcgt ggcgcaattc catcatgcaa atggcggacg tgctggcgga cgacgacatg
     7981 ccggggtcga tgggcgtggg cgtggaactg ggcatcccgc aaaccgcgaa gcgcatcgat
     8041 ttcgtccttt ctggcacatc cgggtcgggc gatccccgcg tgatcatcgt cgagctcaag
     8101 caatggtcga cttcacgcgt ttcggacagg gatggattgg tgatcgccaa ccgcggtggc
     8161 cacgccgaca tcgaaggcat ccacccctgc taccaggctt ggtcgtatgc cgcgctgttg
     8221 gaaggcttca acgaggccgt gcatgggggc gggatcggat tgaacccatg tgcgtacctg
     8281 cacaaccacc atcgcgatgg catcatcgat gcggcacgct acgcgcccta catcgacaag
     8341 gcaccgctct tcctgcgtgg cccgagtgaa cgccggaagc tgcaggattt catcaagcag
     8401 cacgtcaagg acggcgacga tgcgcaagcg ctttaccgga tcgaacacgg tcgcattcgt
     8461 ccatcgaaaa tgctcgccga cagcttgtcc ggcatgctgc gcgggaatcg cgagttcgtc
     8521 ctggtcgacg accagaaggt ggtctacgag acctgcctgg cgaaggccgc catggcgaac
     8581 cccacgcgca aacaggtcgt gatcgtgcaa ggcggccccg ggacgggaaa gtcggtcgtc
     8641 gcgatcaacc tgatggtggc gctgaccagg caaggattgc tggcgaaata catctccaag
     8701 aatgcggcgc cgcgcgcggt gtacgcgcag aaactcaagg gccagcgcaa gcaggccgag
     8761 atctcgatga tgttcggcag ctcgggagcg ttcatcgaca ccgaagcgga cctgttcgac
     8821 gcgttgatcg tcgacgaagc gcaccgtttg aacgaaaaga gcggccttta cggcaatctg
     8881 ggggagaacc agatcaagga actcattgcc gcggcgaagt gcacgatcct gttttgcgat
     8941 gacgaccaga tggtgacctt ggcggacatc gggcattcgg ccgagatcga ccgctgggca
     9001 cgccatctcg gcgccgatgt cacccacctc gcgttggcat cgcagttccg ttgcgcaggc
     9061 tcggatggct acctggcatg gttggacgat gtgctcggcg ttcgcgagac cgccaacccg
     9121 gacctggttc caggcactta cgacttccgc gtcgttgata gccctgccga gttggatcgt
     9181 cttatccgcg agcgcaacgg cagtaacaag tcccgcttgg tcgctggcta ttgctgggac
     9241 tggaagagca agcgcaatcg cgatgcatac gacatcgtcc tcccggcgtc gggcttcagc
     9301 aggcaatgga accttggaag cgacggtagc ctgtggatca ccgcacggga ttcgattgaa
     9361 cagatcggct gcatccatac ctgccaaggc ttggagctcg actatgtcgg cgtgatcatt
     9421 ggccccgatt tcgccatggc gaatggggct ttgatgacga acccggctgg ccgctcgaaa
     9481 atggatcgat ccatacgcgg atggaaggca ctcacgaagc gcgatccggt aggtacccgc
     9541 gaacgactcg acaggatcat ccgaaatacc tatcgcacgc tgatgacgcg agggatgaaa
     9601 ggttgctacg tctattgcac cgatcctgaa gtggcggact tcctgcggga acgtatgcgc
     9661 cgcatgggtt cgtgaccggc ctccgctaga ctccgcaaaa cctcggagcc ccgcatgtcc
     9721 ggccacagcc agttcgacct gctcaagcag cgccgcttcc tgccgtactt cacggtgcag
     9781 gcgctgggcg cgttcaacga caacgtgttc cggcaggcga tcatcgggct gctgggggtg
     9841 atggcggcgg cagggctgct ggacgagacc acgcgcggca cctacacgca gctggcaccg
     9901 gcggtgttca tcctgccgta cttcctgttt tcctcgatcg ccggccagtt cgcggagcgc
     9961 accgagaagt cgaagctgat ccgcatcacc accgcgatgg agatcgcgat catgtcgctg
    10021 gcggcggtgg gcttcctgct gcaggacatg cgggtgctgc tgttcgcgct gttcgccacg
    10081 ggcgtgcaaa gcacgctgtt cgggccggtg aagtattcga tcctgccgtc ggtgctgaag
    10141 ccggaggaac tgaccggcgg caatggcctg gtggaaatgg gcaccagcat cgcggtgctg
    10201 ctgggcatgc tgttcggtgg gttgatcttc accgtggccg gcgaacacgg cacgcaggcc
    10261 gcggcggtcg cggtgatcct gctggcgatc gcgggcaatg tgttgagccg gatgatcccg
    10321 gcggtgccgg cctccgcgcc cgacctggag gtgcagtgga acccgatccc ggaatcgctc
    10381 gccatcctgc gcctggcgaa gaagcaactg gcggtgcgca attccatcct gggcgtgagc
    10441 tggttctggt tcaccggcac cgtgctcacc ggcaacctgc cggtgtatgc ggagacccac
    10501 ctgggcggca cgccgacgct gtacgtgttc gcgctggcgg tgttctcgat cggcgtgggc
    10561 acgggctcgc tgctgtgcga aaaactctcc gcgcgcacgg tggagatcgg cctggtgccg
    10621 ctgggcgcga tcggcatgag cgcctgcatc ctgcaccttg gcttcgcgcc ggcgatcgcg
    10681 gcgaagggcc tggatgtcgg gccgttcctg cagcaggcgg gtgcgtggcg ggtggtgctg
    10741 gacctggccg ggatcggcct gttctccggc ttcttcgtgg tgccgctgtt cgcactgatc
    10801 cagagccgca cgccgaagga cgaactatcg cgcgtgatcg ccggcatgaa catccagaac
    10861 gcagtcttca tcgtcgcggc ggcattgggt ggcgtggccc tgcaacagct cctgggctgg
    10921 agcatcccgc agctgttcct cgcactcggc gtcgccagca tcgtggtggc ggcatggatc
    10981 ttcagcatcg tccccgaatt cctgatgcgc ttcctcagct ggttgctggt gcgcggcctg
    11041 taccggctca agctgcacgg catcgagaag cacgtgcccg acgaaggcgc ggcgctgctg
    11101 gtctgcaacc acgtgagtta catggatgcg ctgatcctgt ccgccagcat tccgcggccg
    11161 gcgcgcttcg tgatgtatta caagatcttc aacatcccgg tgatgcgctg gatcttcagg
    11221 accgccaagg cgatcccgat cgccggcgcg cgcgagaacc cggagttgat gcagcgcgcg
    11281 ttcgacgagg tcgatgcggt cctggccgag ggcgaactgg tctgcatctt cccggagggc
    11341 gcgttgacca aggacggcga catcgcgccc ttcaagtccg gcgtggaacg catcctgcaa
    11401 cgcgccgccg cgcgcggcca ggtcgtcccg gtggtgccga tggccctgcg caacatgtgg
    11461 ggcagcatgt ggagccgccg cgcgaacgcg aaggaaggca gcatgctgga tcgcatgcgc
    11521 gtcccgcggc gcctgcgcgc acatgtggat gtggtggccg gcgagccggt ggatggcggt
    11581 accgcgacgg cggaattgct ggaggcgaag gtgcgggcgt tgcgcgggga tgcggcctaa
    11641 cccacccagc ccgccaccac gaacagcgcg atcaccgcca gccacagcac cagtacgcgc
    11701 caggccaggt tcatcgcatc gcgcagttcc ggcaattcgc cgaagacttc ggcgagtgcg
    11761 gtggaggccg aaatgccgga ttccgtgtag tcggccactt cctcggcgat ctcgctgcgc
    11821 acgctggcgc gggcggcgtt cggcaacagg cggctgtcca ggccgaaggc atcgtcctgc
    11881 cgccacgccg agagcacgct gtcgaaattg ccgaccagcg ccagccccag cgcgatcaac
    11941 tgggtcaccg gccattccag cagcgacagc cagacgcgcg cgccgtgcag ggtgtcggat
    12001 ggcatgacgt cggcgtcctg ctcggcggcg agcacgctca ggcgatacag cagcgcgccg
    12061 aaggggccca gcacgcagaa ccagaacagc acgccgaacc agcgccgctg cgcattgcgg
    12121 aacacggcct cgaccagcga ggcgccatcg agcggcgcat cgacgccggg cggatacagg
    12181 cgtgccgcgg cggcgcggcg cgacgtggcg tcgggcgcgt cgagcacggc ggcgacgtcg
    12241 agatcgaggt cgcgcgggcc ccagcaccag aacagcagga cgatgtcgaa cagcaggccg
    12301 acgaagcccc acagcggctg gtccagcgcg acctgcaaca ggcccgcgac gagcaacggc
    12361 acgaccagtg ccagcgcgat gccccagcga ccgcgccaga aactgtcttc ggggaattgc
    12421 gcgttgagcc attgcagcca gctgcgatac cagccgaact ggcgcaggcc attggcgatg
    12481 cccggcgcca ggtggccgat ggccagcgcg atgatgacgg cgagcaacgt ggcggacatc
    12541 gcccgattct actgcgcgat gtcgctacga cgcggcgcgc gcgtcgtgct ccgccagcca
    12601 cgcgtggatc acgtggcggg cgatcgagat cggcgaggac aacacgatgc cctcgccttc
    12661 cgcatcggcg ctcgcccagt cgttggccag gcccacgcgc acctcgtcgg cgctgaacca
    12721 gcgggcagcc tcaagttctt cttcggccac cggctcatcg ggttcggcgt tggcgatgaa
    12781 gcccagcatc aacgagccgg ggaacggcca cggctgcgag gcgacgtaac gcgccgtgtg
    12841 cgcatgcacg cgcacgccgg tttcctcgtg cacctcgcgc accacggtct gctccagggt
    12901 ttcgccgggt tcgacgaagc cggcgaccac cgaccagcgt cgcggcggcc agctcgcctg
    12961 gcgtcccagc agcagccgct cgccatcggt gaccgcgacg atcaccgcct ggtcggtgcg
    13021 cggatagtgc tcactgttgc aggcctcgca gcggcccagc cagccggcgc ggatgaagcg
    13081 caaccgaccg ccgcaggcgc tgcagtggcg atgccgctgg tgccagtgct gcagcgcgcg
    13141 cgcctgcgcg aacagggtgg cctgccaggc cggccattgg gcggcggcgc gacgcaggtc
    13201 aatgcggtcc ggcgcggagg ccgcgagggt gctggcatca gcgaagaacc acgcctggcc
    13261 ctcgcgcagg ccaaggaagg tcatggtgcc ggggccattg ccgagcgcgg cagcgtccag
    13321 cgccagcaag ccgcgatcgg catcggcgaa ggcacggcca tccgtgtcca gcacgatcac
    13381 ctgcgcctgc ggccatagcg cctgcagggc gtcggcatcg ccgcgcaggt ggtcggcgcg
    13441 atcgagcgcg ccatcgacga acgaaaaagg aaagggggtc agagtcactt tgcctgccca
    13501 cggaatcggc acgcggggat cgcgtgggaa aagtgactct ggcccccttt cctcctcaca
    13561 ccgtgaagct gctgccgcaa ccgcaggtgg tcttcgcgtt cgggttgcgg atcacgaact
    13621 gcgcgccggt cagcg
//
LOCUS       DAOOCX010000063        13543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_4182_length_13543_cov_7.769721, whole genome shotgun sequence.
ACCESSION   DAOOCX010000063 DAOOCX010000000
VERSION     DAOOCX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 13543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13543
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4182_length_13543_cov_7.769721"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1613)
                     /locus_tag="PK399_07380"
     CDS             complement(39..1613)
                     /locus_tag="PK399_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HPW12976.1"
                     /translation="MQRKCLPILALALAGWILLPGIGDAATPAANAPSLRIHTASGVV
                     EGVRDDGLAVFKGLPYAAPPLGALRWRAPQAAPSWRGVRKADAFGNVCMQVPGAARAA
                     GAGDVGPMGEDCLTLNVWTPGTAGATRPVMVWIHGGALVFGAGSQAPYAGNALAERGA
                     VVVTLNYRLGPLGFFDHPALAAGSGGDVNFGLLDQVAALKWVRDNIAAFGGDPGNVTI
                     FGESAGGQSVLALFASPKARGLFQRGIAQSPYGIPSHTRAKARAVSVEIASASGLDGS
                     RATGEQLRAIPAERLSQAGAIKGNSLAPGFAVGDTALPQPILEAFQQSRQAKLPLIIG
                     SNSDEATVATAFGLDPAAVVERLGAGRILLKPLYPGVSDDAELGRQAIRDLVFTAFAR
                     RIAYLHSQRAPTWRYYFNHVQDGLRPAPAGVGHGGEIVYVMGTADACRCLAVPFSTAD
                     RKVSRDIGDYWFAFARNGVPAAAGAPAWPKDSAKRAQVLEFADTPTPRANFMQARLNV
                     MIGALKGIGGYLGKKE"
     gene            1738..2214
                     /locus_tag="PK399_07385"
     CDS             1738..2214
                     /locus_tag="PK399_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HPW12977.1"
                     /translation="MQDLFQLSAPWWHFVLRACAIYALVMVLVRVSGKRAVGQFTPFD
                     LVLLILIGNAVQNGINGGDNSLTGAAIMATTLIALNYAVAYVTSRNRKVEKFVEGVPV
                     LLARDGQLFEAVLRRELVSREDFREALRMNGVDDVAEVELALLETNGSISVVKKRD"
     gene            complement(2236..3168)
                     /gene="hemF"
                     /locus_tag="PK399_07390"
     CDS             complement(2236..3168)
                     /gene="hemF"
                     /locus_tag="PK399_07390"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HPW12978.1"
                     /translation="MPTFDIVRDALTDLQDRICAAIEAADGSARFIEDPWQRDPDDAS
                     THLRGGGRTRILRDGAVFEQAGIGFSDVSGTKLPPSATAARPELIGASWRAVGVSLVF
                     HPRNPYLPTTHANVRHFRAVRDGETVAWWFGGGFDLTPFYPFDDDVRHWHRTARDLCE
                     PFGGAARYDAHKRWCDEYFFLRHRNETRGVGGLFFDDLHGDFADDFGYMQAVGNGFLD
                     AYLPIVERRKATPFGERERQFQLYRRGRYVEFNLVFDRGTLFGLQSGGRTESILMSLP
                     PLVRWEYGFTPEAGSQEARLADYLRPRDWLGEST"
     assembly_gap    3258..3357
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3365..4615)
                     /locus_tag="PK399_07395"
     CDS             complement(3365..4615)
                     /locus_tag="PK399_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HPW12979.1"
                     /translation="MTLFRSVLFWLVLAVLGALFAQILLQDPGYVLVRFRGTDYETTV
                     AVAFGLVAGAMLCMALAWTLLRLPFRAWRRHRARQARAKLTDGFAAYRRGDYARAEQL
                     LAQAADSGDAEALARTQAARAALARGDEAAANAHLEAFGDRHPAARALQRARQALALG
                     RPGEALLALDVPAAQPLPPSGLRLRAEALAASGRSIEAYGLLGALRQQQALPAATLDA
                     LQREWAAQSLQQAADANALADRWDALPEPLRTSPPVVSAYASRAAAMNWDEAATRSLE
                     HALDAQWDDGLVALYGQLPVGRLEHRRAQVERWLPAHPSSPGLLLAAARVAHAQGQWP
                     QAEAWLHRAIAQGAGAPAWELLGDGAARNGDDAGARMAYANALRSQRGEPALDVPGRG
                     LRQQIADTAAVEERNEHGLPSLRG"
     gene            complement(4695..5603)
                     /locus_tag="PK399_07400"
     CDS             complement(4695..5603)
                     /locus_tag="PK399_07400"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010370612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPW12980.1"
                     /translation="MLWLLLALVLAVAGAFGWQQWQARERELRSLAEQQRAALDARVE
                     TLLQAQRAQAQRLQQAEATNRVLRDELLGIGQRAALLEDSVSKLADPDRHGAQALRLD
                     EIELLLAIGQQRLRLDDDIDGARRALALAAPLLEGLDDPAYLNLRQALAQEEAALHAL
                     GADPRIQANALLERIADGIDATSPVRPTPSRHAPWYQRLLDRLVRRQPTASAGLQQHP
                     DREAALAALQVELSLARAAVERRDAAGLRRALARVDGWLQRLLAGSPGLPRKRRLLAE
                     LQALPLRMESPLAGTTLQQLRAVRGQ"
     gene            complement(5676..6455)
                     /locus_tag="PK399_07405"
     CDS             complement(5676..6455)
                     /locus_tag="PK399_07405"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPW12981.1"
                     /translation="MIRRASRAAPLAGWTVLSLRPRGQHAGLRAAAARMGARVLAMSA
                     LASEARDDDASRNALAASLTADIVLFTSPNAVRAAATLRHLRIMRGRTVLAVGDGTRR
                     ALQRVGIAAMSPTRMDSEGLLAMPALHDVAGRSVGLVTAPGGRNRIGPELQARGATIR
                     RADVYERVPVAIPAARWRAMAEALAEPRSVLLALSSAEAFDAMLRQQPADLAKPLRRI
                     AVTAASERLAQAARDAGFRRVAIAAGARPASLLRAAADAFV"
     gene            6501..7007
                     /locus_tag="PK399_07410"
     CDS             6501..7007
                     /locus_tag="PK399_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HPW12982.1"
                     /translation="MQSPSLSSSSPSPSPSPLLDALAAHYMAMPPVAAMQPRVLDWED
                     GCLRMQAPLDANVNDKGCAFGGSLSSLMTIAGWGLAFLRLAEAGLEADIYVADSRVRY
                     LKPVYEDLRVEVRLDTAGESAADAIDLPGALRAKGRASVRMEARTLLADGGVAAVLVG
                     RYVAIARG"
     gene            7063..7485
                     /locus_tag="PK399_07415"
     CDS             7063..7485
                     /locus_tag="PK399_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12983.1"
                     /translation="MPRLLLRCLLLCCLALLAGCPKTHASKGSALDEVQYDYSAAIRW
                     GDFEGAWNLVDPKVRAEKPLTDIDFSRYKQIKISGYTDLGGTVEPSGVVVRDVEIGVI
                     NLNTQAVRTVRYRERWRYDEAAKTWWLLSGLPDLWQGD"
     gene            7532..7966
                     /locus_tag="PK399_07420"
     CDS             7532..7966
                     /locus_tag="PK399_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPW12984.1"
                     /translation="MTLAELVAFAGRHPLLSLALAGITLAILYNEIAGRFSGFRRIGP
                     AQLTGMINRDDALVVDLRPAADFEKGHIAGAKNVQMAQFDPENKQLAPAKALPVVLVC
                     KSGETASGAAKRLRKAGFANVNVLEGGIQAWQGADLPLVKGR"
     gene            8107..8628
                     /gene="secB"
                     /locus_tag="PK399_07425"
     CDS             8107..8628
                     /gene="secB"
                     /locus_tag="PK399_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HPW12985.1"
                     /translation="MSDEILNGAAPLADAQPQGATFTVEKLYVKDLSFEAPNAPQVFN
                     EQGQPDLQMKLNQKVQRLSDNAFEVSLGITLTCTINGRTAYLAEVEQAGVFGLGGFED
                     HILDAMLGTQCPNILYPYASAAVGQLIVSGGFPPFPMQPINFDALYGETLRQRAAQQA
                     GGELANTETAGNA"
     gene            8641..9672
                     /locus_tag="PK399_07430"
     CDS             8641..9672
                     /locus_tag="PK399_07430"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPW12986.1"
                     /translation="MTQSRKPSVAVLGAGSWGTALAALIARHGHSTVLWGRDAATISA
                     IEAEGENPRYLPGIPLPGNLRATTALPEAMANADLVLVVVPSHAFAETLRALAPWREP
                     GAGVAWATKGFEPGSGRFLHEVAGEVLGDDVPLAVVTGPSFAKEVAEGLPTALTVHSE
                     DAVFCQQVADALHGPAFRAYTGDDMCGAELGGAMKNVLAVATGVADGMGLGLNARAGL
                     ITRGLNEMLRLNIGIGGRPETLMGLAGLGDLVLTCTGDLSRNRRLGLALGRGQSIEDA
                     VRAIGQVVESIQTADEVMRLAKRHGIELPIAENVRDVLHGDITPAEGLKRLMARERKA
                     EYPQSLFGD"
     gene            complement(9795..10376)
                     /locus_tag="PK399_07435"
     CDS             complement(9795..10376)
                     /locus_tag="PK399_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diffusible signal factor-reguated Ax21 family
                     protein"
                     /protein_id="HPW12987.1"
                     /translation="MKRSLLALTLLAALPFAASAADGLSYNYVQGGYTHVNSDINANG
                     WAIDGSAAIAPNFHVFGGYNTVKADTIVLPAGSYKPKLDQWKLGVGYNHAIATNTDFV
                     GTLAYQKAEAKLAGVNANVDGYAAEAGVRSALGSRVEGYAMAGYEDGNDFDGDFYGRL
                     GGQVKINANWGVAADVKFADGDTQYFVGPRITW"
     gene            complement(10526..11005)
                     /locus_tag="PK399_07440"
     CDS             complement(10526..11005)
                     /locus_tag="PK399_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HPW12988.1"
                     /translation="MFHVILHRPEIPPNTGNVIRLCANTGAQLHLVRPLGFDLDDKQL
                     RRAGLDYHEYARMQVHDDLDAALGAIARANGEPPRVFALSTRGTVRFDTPAYRAGDAF
                     LFGSETAGLPQAVLDAIPDGQRLRLPMRPGNRSLNLSNTVAVVVFEAWRQSGFDGGA"
     gene            complement(11019..11756)
                     /locus_tag="PK399_07445"
     CDS             complement(11019..11756)
                     /locus_tag="PK399_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW12989.1"
                     /translation="MARTALVTGANRGIGLEFVRQLLARGDHVVATCRHPGKASALNA
                     LAGDHPARLHVLPLDVADAKSRASLVHDLPLVLGDDGRIDLLLNNAGVLHSGERFGHV
                     EQATLDDSLRTNAIGPFLLAQALAPLLRDGTVAVDDAASPCTIIANISSQLGSITHTA
                     RFGTPSYAISKAAQNMASALLANALAGRGIVVLALHPGWVQTEMGGAQAQVTPVDAVA
                     GLLRVIDAATPSQSGSFLDWRGEPLPW"
     gene            11853..>13543
                     /locus_tag="PK399_07450"
     CDS             11853..>13543
                     /locus_tag="PK399_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPW12990.1"
                     /translation="MSVFRPAVLAVALATALSAGTFAPQARAATAPVVDIAYDSFTLP
                     NGLRVIVHTDRKAPIVAVNVWYHVGSKDEPAGRSGFAHLFEHLMFNGSENNPGEFFGP
                     FKQVGATNQNGTTNSDRTNYFENVPTTALDMALWMESDRMGHLLGAIDQKTLDEQRGV
                     VQNEKRQGQNQPYGQVWDVLGKAMYPVGHPYNHSTIGSMNDLNAASLDDVKTWFKTWY
                     GPNNAVLVLAGDIDLATAKEKVAKYFGDIPAGPTMAQPKVDVAAHTKDSRGEMTDNVP
                     QSRIYRMWNVAEYGQPDLDRLQLFAQVLGGGKSSRLDRRLVHQDKLVDNVGASAFGSQ
                     LGSNFMITAAVKQGVDVAKVEAAIDEELQKLLKDGPTADELARARTSIQAGWIRGVER
                     IGGFGGKADALAECAVYTGDPGCFRDSLKTLGDATVASVRATGNTWLGAGKGSHTLVV
                     NPGKRVDIPEEPAVTPAPFKLPAVDKKYATTASAVDRKAGVPVTTSFPDLKFPSLQRA
                     TLKNGTKVVLAERHDIPVVQMSYEFRGGQSADPAAKLGTASFAMGLLDEGAGDLDA"
BASE COUNT         2072 a         4630 c         4742 g         1999 t
ORIGIN      
        1 tcgtggcgga gaattgaagc gctatcgggt ctgggcggct attccttttt gcccaagtag
       61 ccgccgatgc ccttcagggc gccgatcatc acgttgaggc gtgcctgcat gaagttggcg
      121 cgtggggttg gggtgtcggc gaactcgagg acttgcgcgc gcttcgcgga atcctttggc
      181 caggcaggtg cgccggccgc tgccggcacg ccattgcggg cgaacgcgaa ccagtagtcg
      241 ccgatgtcgc gcgagacctt gcgatccgcg gtcgagaagg gaacggccag gcatcggcat
      301 gcatcggcgg tgcccatcac gtagacgatc tcgccgccgt ggccgacgcc agcgggtgcg
      361 ggccgcaggc cgtcctgcac gtggttgaag tagtagcgcc aagtcggtgc gcgttgggag
      421 tgcaggtagg cgatgcgccg ggcgaatgcc gtgaacacca ggtcgcggat ggcctgccga
      481 cccagttccg catcgtccga taccccggga tacagcggct tgagcaggat cctgccggcg
      541 cccaggcgtt ccacgactgc ggcagggtcc agcccgaacg cggtcgccac cgtcgcttcg
      601 tcgctgttgc tgccgatgat cagtggaagc tttgcctgcc ggctttgctg gaaggcttcc
      661 aggatcggct gcggcagcgc ggtgtcgccc accgcgaagc ccggtgccag tgaattgccc
      721 ttgatcgcgc ctgcctggct gagccgttcc gccgggattg cacgcagttg ctcccctgtc
      781 gcgcgcgatc cgtccagtcc gctggcggag gcaatctcca cggagacggc acgtgccttc
      841 gcccgcgtgt ggctggggat gccgtaggga ctttgcgcga tgccgcgctg gaacaggccg
      901 cgcgccttcg gcgaggcaaa cagcgcgagc acgctttggc cgccggccga ctcgccgaag
      961 atcgtcacgt tgccggggtc gcccccgaag gccgcgatgt tgtcgcgcac ccatttcaac
     1021 gcggcgacct ggtcgagcag gccgaagttc acgtcgccgc ccgaaccggc ggcgagcgcc
     1081 ggatggtcga agaacccgag tggccccaac cggtagttca gggtgaccac gaccgcgccg
     1141 cgttccgcca gtgcgttccc ggcatacggg gcctggctgc ccgcgccgaa caccagtgcg
     1201 ccgccatgga tccacaccat caccgggcgg gtcgcgcctg cggtcccggg tgtccagaca
     1261 ttgagggtca ggcaatcctc gcccatcggt ccgacatcgc ccgcgccggc agcgcgcgca
     1321 gcgcccggga cctgcatgca gacgttgccg aaggcatcgg ccttgcgcac gccgcgccag
     1381 gacggtgcgg cttgcggtgc gcgccagcgc agcgcaccaa gcgggggcgc ggcgtagggc
     1441 aggcccttga acaccgccag gccgtcatcg cggacgcctt ccacgacccc ggatgcggtg
     1501 tggatccgca acgacggcgc gttcgctgcc ggagtcgcag cgtcaccaat gccggggagg
     1561 aggatccagc cggcaagcgc cagcgcgagg atgggcaagc atttgcgttg catgcggtca
     1621 ctccgttccg ggcgcttgcg gtcgagtata ggagccatcc ccaggttcgc cgcatgagcg
     1681 cgacatcatg cgcctgtggg atgctcttgc aggttcggca gccgcgagcg cattcccatg
     1741 caagacctgt tccagttgtc cgcgccatgg tggcatttcg tgctgcgcgc ttgcgcgatc
     1801 tacgcactgg tgatggtgct ggtgcgcgtg tccggcaagc gcgccgtggg gcagttcacc
     1861 ccgttcgacc tggtgctgct gatcctgatc ggcaacgcgg tgcagaacgg catcaacggc
     1921 ggcgacaact cgctgaccgg cgcggcgatc atggcgacca cgcttatcgc cctgaactac
     1981 gccgtggcct acgtgacctc gcgcaaccgg aaggtggaga agttcgtgga aggcgtgccc
     2041 gtcctgctgg cccgcgatgg ccagctgttc gaggccgtgc tgcgccgcga gctggtcagc
     2101 cgcgaggact tccgcgaagc cctgcgcatg aacggcgtgg acgacgtggc cgaggtcgag
     2161 ctggccttgc tggaaaccaa cggcagcatc agcgtggtga agaagcgcga ctgatcaggc
     2221 gcgcgcgtcg gacgctcagg tggattcgcc gagccagtcg cgcggacgca ggtagtccgc
     2281 caaccgggct tcctggctcc cggcttccgg cgtgaagccg tactcccaac ggaccagcgg
     2341 cggcaggctc atcaggatcg actcggtgcg gccgccgctc tgcaggccga acagggtgcc
     2401 gcggtcgaac accaggttga attcgacgta gcgtccacgc cggtacagct ggaactggcg
     2461 ctcgcgctcg ccgaatgggg tcgccttgcg gcgttcgacg atcggcaggt aggcatccag
     2521 gaagccattg ccgaccgcct gcatgtagcc gaagtcgtcg gcgaagtcgc catgcaggtc
     2581 gtcgaagaac aggccgccga cgccgcgcgt ctcgttgcgg tgcctgagga agaagtactc
     2641 gtcgcaccag cgcttgtggg cgtcgtagcg cgccgcgccg ccgaacggtt cgcacaggtc
     2701 gcgggcggtg cggtgccagt gccgcacatc gtcgtcgaac gggtagaacg gagtcaggtc
     2761 gaagccgccg ccgaaccacc aggccacggt ttcgccatcg cgtaccgcgc ggaagtggcg
     2821 cacgttggca tgcgtggtcg gcaggtacgg attgcgcggg tggaatacca gcgacacccc
     2881 gacggcgcgc caggaggctc cgatcagttc cgggcgcgct gcggttgccg atggcggcag
     2941 tttcgtcccc gacacgtcgg aaaacccgat ccccgcctgc tcgaagaccg caccatcgcg
     3001 gaggatgcgg gtgcgcccgc cgccgcggag atgggtggag gcatcgtcag gatcgcgttg
     3061 ccacggatcc tcgatgaagc gcgcggaacc atccgcggct tcgatcgcgg cgcagatgcg
     3121 atcctgcagg tcggtgaggg cgtcgcggac gatgtcgaag gtggggatcg tggtcatgcg
     3181 gccattatcg ccgcaggcca cggcgtgcat cagccgcgca ggctcggcag tccgtgctcg
     3241 ttgcgttcct cgaccgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     3361 tgcatcagcc gcgcaggctc ggcagtccgt gctcgttgcg ttcctcgacc gcggcggtgt
     3421 cggcgatctg ctggcgcagc ccgcggccgg gcacgtcgag tgcaggctcg ccgcgctggc
     3481 tgcgcaacgc atttgcatag gccatgcgcg cgccggcgtc gtcgccattg cgggccgcac
     3541 catcgcccag cagttcccac gcgggcgcgc ctgcaccctg cgcgatcgcg cgatgcagcc
     3601 acgcctccgc ctgcggccac tgtccctgcg catgcgccac gcgtgctgcg gccagcagca
     3661 gcccggggct ggacggatgg gcgggcagcc agcgttcgac ctgggcgcgg cgatgctcca
     3721 ggcggccgac cggcagctgc ccgtagagcg cgacgagacc gtcgtcccac tgcgcatcca
     3781 gtgcgtgttc gaggctgcgg gtggcggctt cgtcccagtt catcgctgcg gcgcgcgatg
     3841 cgtaggcgct gacgaccggg gggctggtgc gcagcggctc gggtagtgca tcccagcgat
     3901 ccgccagtgc gttggcgtcg gcggcctgtt gcagcgattg cgcggcccat tcgcgctgca
     3961 acgcatccag cgtcgcagcc ggcaaggcct gctgttgccg caacgcgccg agcaggccat
     4021 aggcctcgat gctgcggccg ctggcggcca gcgcctcggc gcgcaagcgc agcccgcttg
     4081 gcggcaacgg ttgtgctgca gggacatcca gcgcgagcag ggcctcgccg gggcgaccca
     4141 aggcgagcgc ctggcgggcg cgctgcaacg cgcgtgccgc cggatggcga tcaccgaagg
     4201 cctccaggtg cgcattggcg gccgcttcat cgccccgcgc cagcgcagcg cgtgcggcct
     4261 gcgtgcgcgc cagcgcttcg gcgtcgccac tgtcggcggc ctgcgccagc aattgttcgg
     4321 cgcgggcgta gtcgccgcgg cggtaggcgg cgaaaccgtc ggtcagcttc gctcgggcct
     4381 gccgcgcgcg atggcgccgc caggcgcgga agggcaggcg cagcagggtc cacgccagcg
     4441 ccatgcacag catcgcgccc gcgaccaggc cgaaggcgac ggcaacggtc gtctcatagt
     4501 ccgtcccacg gaagcgcacc agcacgtaac cggggtcctg caacaggatt tgcgcaaaca
     4561 gcgcgccgag tacggcgagc accagccaga acagcacgct gcggaacagg gtcatcgcgc
     4621 ctccttgcgg ttaccggatc agcgttgtcg cgagcatcgc agcggcgatg caagcgctgt
     4681 gtgagcgcgt tgcatcactg cccgcgcacg gcgcgcagtt gctgcagggt ggtgccggcg
     4741 agcggtgatt ccatgcgcag cggcaaggcc tgcaattcgg ccagcagccg gcgcttccgc
     4801 ggcagtccgg gcgagcccgc caacagccgt tgcagccaac cgtcgacgcg cgccagcgcc
     4861 cggcgcaagc cggcggcatc gcggcgttcc accgcggcgc gcgccaggct gagttccacc
     4921 tgcagcgcgg caagcgcggc ttcgcgatct ggatgctgtt gcaggccggc cgacgcggtc
     4981 ggctggcgac gcacgaggcg atccagcagg cgctggtacc agggggcatg ccgcgagggc
     5041 gtgggtcgga ccgggctggt cgcgtcgatg ccgtcggcga tccgctccag cagggcattg
     5101 gcctggatgc gcggatccgc gcccagcgcg tgcagcgcgg cctcttcctg cgccagggcc
     5161 tgccgcaggt tgagataggc cggatcgtcc aggccttcaa gcagtggcgc cgccagcgcc
     5221 agtgcgcggc gtgcgccgtc gatgtcgtcg tccaggcgca agcgttgctg gccgatggcg
     5281 agcagcagtt cgatctcgtc caggcgcagt gcctgcgcgc cgtggcggtc ggggtcggcc
     5341 agcttcgaca cgctgtcttc cagcagcgcc gcgcgctggc cgatgccgag cagttcgtcg
     5401 cgcagcacgc ggttggtcgc ttcggcctgt tgcaggcgtt gtgcctgcgc gcgttgcgcc
     5461 tgcagcagcg tctccacgcg cgcatccagc gccgcccgct gctgttccgc caacgaacgc
     5521 aattcccgct cgcgtgcttg ccattgctgc cagccgaaag cgccagccac cgccagcacc
     5581 agggcgagca acaaccacag caatgcggct ccggcgagcg aacggcgcgc gggtgcgacg
     5641 gacgggagag gcgtcggcgt gctcaccgga cgatgctaaa cgaaggcatc ggcggcggcg
     5701 cgcagcagcg aggccgggcg cgcgccagcg gcgatggcca cgcgccggaa gccagcgtcg
     5761 cgcgcggcct gcgccaagcg ctcgctggcg gcggtcaccg cgatccgtcg caacggcttc
     5821 gcgaggtcgg cgggttgctg gcgaagcatc gcgtcgaaag cttcggcgct gctcaatgcc
     5881 aacagcaccg atcgcggttc agccagcgcc tcggccatgg cccgccagcg cgctgcaggg
     5941 atcgctaccg gcacgcgctc gtagacatcg gcgcgccgga tcgtggcacc acgcgcctgc
     6001 aattcgggcc cgatccggtt gcgtccgccc ggtgcggtca ccagcccgac cgaccgaccg
     6061 gcgacgtcgt gcagtgccgg catcgccagc aggccctcgc tgtccatgcg ggtgggggac
     6121 atcgccgcga tcccgacccg ctgcaaggca cgacgggtgc catcgcccac cgccagcacg
     6181 gtgcgtccgc gcatgatgcg caggtggcgc aaggtggctg cggcgcgcac cgcattggga
     6241 ctggtgaaca acacgatgtc ggcggtgagt gacgcggcga gcgcgttgcg gctggcgtcg
     6301 tcgtcgcgcg cctcgctcgc cagcgccgac atcgccagca cgcgggcgcc catgcgtgcg
     6361 gcggcggcac ggaggcccgc atgctggccg cgcgggcgca gcgacagcac ggtccagccg
     6421 gcgagcggcg ctgcgcggga ggcacgtcgg atcatccgta cagcttggca tggcgctgcc
     6481 gcgtgcgtca ggctgtcgcg atgcagagcc cctcattgtc ctcctcctct ccctccccct
     6541 cgccctcccc actgctcgat gcactggccg cgcactacat ggcgatgccg ccggtcgcgg
     6601 cgatgcagcc gcgcgtgctc gactgggagg acggttgcct gcgcatgcag gcaccgctcg
     6661 acgccaacgt caacgacaag ggctgcgcgt tcggcggtag cctgagctcg ctgatgacca
     6721 tcgccggctg gggcctggcg ttcctcaggc tggccgaagc cggcctggag gccgacatct
     6781 acgtcgcaga cagtcgcgtg cgctacctca agccggtcta cgaagacctg cgggtcgagg
     6841 tgcgcctgga cacggccggc gaaagcgctg ccgatgccat cgacctgccc ggcgcgctgc
     6901 gcgccaaggg gcgtgccagc gtacgcatgg aggcgcgcac cctgctggcc gacggcggcg
     6961 tggcggcggt gctggtcggc cgctacgtcg ccatcgcccg cggctgagcc cgcctgcggt
     7021 cgtcgagcgg gtaggatgcg gacacccacc ggaggaaccg cgatgccacg actgttgctg
     7081 cgctgcctgc tgctgtgctg cctcgccctg ctcgcgggct gtcccaagac ccatgcgagc
     7141 aagggcagcg cgctggacga ggtgcagtac gactattccg cggccatccg ctggggcgat
     7201 ttcgagggcg cgtggaacct ggtggatccg aaggtccgcg cggagaagcc gctcaccgat
     7261 atcgattttt cgcgctacaa gcagatcaag atttccggct acaccgacct gggcgggacc
     7321 gtcgagccga gcggcgtggt cgtgcgcgac gtggagatcg gcgtgatcaa cctcaacacc
     7381 caagccgtcc gcaccgttcg ctaccgcgag cgctggcgct acgacgaggc ggccaagacc
     7441 tggtggctgc tcagcggcct gccggacctg tggcagggcg attgaccgcg gcacgggtac
     7501 aatccacgcc ccgcaccatc cggtcctgcc cgtgacgctt gccgaactgg tcgccttcgc
     7561 cggccgccac ccgctccttt cgctggccct ggccggcatc accctggcca tcctctacaa
     7621 cgagatcgcc ggccgcttca gcggtttccg gcggatcggc ccggcacagc tgaccgggat
     7681 gatcaatcgc gatgacgccc tggtggtgga cctgcgtccc gccgcggact tcgagaaagg
     7741 ccacatcgcc ggcgcgaaga acgtgcagat ggcccagttc gatcccgaga acaagcagct
     7801 ggcgccggcc aaggcgctgc cggtggtgct ggtctgcaag tccggcgaga ccgccagcgg
     7861 cgcggcgaag cgcctgcgca aggccgggtt cgccaacgtc aacgtgctcg agggcggcat
     7921 ccaggcctgg cagggcgcgg acctgcccct ggtcaagggc cgctgagcca aaggggccgg
     7981 cttggcccgg cgcttgcgaa accgccggcc cgccccaact caggcagcaa gcgcctcgcg
     8041 gtgcgataat cgccgtcttc atttccgcgc gccccaccgc gcatccatgc aattcctgga
     8101 gccaccatgt ccgatgaaat cctgaacggc gctgccccgc tcgccgacgc ccagccgcag
     8161 ggcgcgacct tcaccgtcga gaagctgtac gtgaaggacc tgtccttcga agcgccgaat
     8221 gcgccgcagg tgttcaacga acagggccag ccggacctgc agatgaagct caaccagaag
     8281 gtccagcgcc tgtcggacaa cgcgttcgaa gtcagcctgg gcatcaccct gacctgcacc
     8341 atcaatggtc gcaccgccta cctggccgaa gtcgagcagg ccggcgtgtt cggcctgggc
     8401 gggttcgaag accacatcct ggatgcgatg ctcggcaccc agtgcccgaa catcctgtat
     8461 ccgtacgcct cggctgcggt cggccagctg atcgtctccg gcggcttccc gccgttcccg
     8521 atgcagccga tcaacttcga cgcgctgtac ggcgaaaccc tgcgccagcg tgccgcgcag
     8581 caggccggcg gcgagctggc caacaccgaa accgccggca acgcgtaagc cggcgactgc
     8641 atgacgcagt cccgcaaacc gtcggtcgcg gtcctcggcg cgggctcctg gggcaccgcg
     8701 ctggcggcgt tgatcgcccg gcacggccat tcgaccgtgc tgtggggtcg ggatgcagcc
     8761 acgatctccg cgatcgaagc ggagggggaa aatccccgct acctgcccgg cattcccttg
     8821 ccgggcaacc tgcgcgcgac caccgcactg cccgaggcga tggccaatgc cgacctggtc
     8881 ctggtcgtcg ttccttcgca tgcctttgcc gaaaccctgc gcgcgctggc gccgtggcgc
     8941 gagccgggcg cgggcgtggc gtgggccacc aagggcttcg agcccggcag cggccgtttc
     9001 ctgcatgagg tcgctggcga ggtgctgggc gatgacgtgc cgctggccgt ggtgacgggc
     9061 ccgtccttcg ccaaggaagt ggccgaaggc ctgccgaccg cgctgaccgt gcattcggag
     9121 gatgcggtgt tctgccagca ggtcgccgat gcgctgcacg gtcccgcgtt ccgtgcctac
     9181 acgggcgacg acatgtgcgg ggccgaactg ggcggcgcaa tgaagaacgt gctggccgtc
     9241 gccaccggcg tcgccgacgg catgggcctg ggcctgaacg cccgcgccgg cctgatcacc
     9301 cgcggcctca acgagatgct gcggctcaac atcggcatcg gtggccgacc ggaaaccctg
     9361 atgggcctgg ccgggctggg cgacctggtg ctcacctgca ccggcgacct gtcgcgtaat
     9421 cgtcgcctgg gcctcgcgct gggtcgtggc cagtcgatcg aggatgcggt ccgggcgatc
     9481 ggccaggtgg tggaatcgat ccagaccgcc gacgaagtga tgcgcctggc gaagcgccac
     9541 ggcatcgaat tgccgattgc cgaaaacgtc cgtgatgtcc tccacggcga catcaccccg
     9601 gccgagggcc tcaagcgcct gatggcgcgc gagcgcaagg cggaataccc gcagtcgctg
     9661 ttcggcgact gatcgccctg cgtgcggctg ccgtcgcgcc tggtcgtggg acgaagaaaa
     9721 aaagcccggc atctgccggg cttttggttg gcgggaatcc cctggaggac gcccgccggt
     9781 gcttcggtgg tcgcttacca ggtgatgcgc ggaccgacga agtactgggt gtcgccatcg
     9841 gcgaacttca cgtccgcggc cacgccccag ttggcgttga tcttcacctg accgccaagg
     9901 cggccgtaga agtcgccgtc gaagtcgttg ccgtcttcgt agccggccat cgcatagcct
     9961 tcgacgcgcg aaccgagcgc gctgcgcacg ccggcctcgg cggcataccc gtcgacattg
    10021 gcgttgacgc cggccagctt ggcttcggcc ttctggtacg ccagggtgcc gacgaaatcg
    10081 gtattggtcg caatggcgtg gttgtagccg acgcccagct tccactggtc cagcttcggc
    10141 ttgtagctgc cggccgggag cacgatcgtg tccgccttca cggtgttgta gccaccgaag
    10201 acatggaagt tcggggcgat ggcggcggag ccgtcgatgg cccagccgtt ggcattgatg
    10261 tcggaattga cgtgggtgta gccgccctgg acatagttgt aggacaggcc gtcggccgca
    10321 gaggcggcga aggggagggc ggccagcagg gtcagcgcaa gcagggaacg cttcatgggg
    10381 gaaacctcat cgagttgttg ttattgcgcc ctgcggggag gtcaggacgt gggcgcattc
    10441 tgggcagggc gtcacaaccc gggatgaata caaaaccatc cggtacagga actattccgg
    10501 ttcgatctgg tcgcgatcgc gccgttcagg cgccgccatc gaagccggac tgccgccagg
    10561 cctcgaacac caccactgcc acggtattgg acaggttgag gctgcggttg cccgggcgca
    10621 tcggcaggcg caggcgttgg ccgtcgggaa tcgcatcgag gacggcctgc ggcagaccgg
    10681 cggtctcgct gccgaacagg aaggcgtcgc cggcccggta ggccggggtg tcgaagcgga
    10741 ccgtgccgcg ggtggacaac gcgaacacgc gcggcggctc gccattcgcc cgggcgatgg
    10801 cgcccagcgc tgcatcgagg tcgtcgtgga cctgcatccg cgcgtactcg tggtaatcca
    10861 gcccggcccg gcgcaactgc ttgtcgtcga ggtcgaaccc cagcggccgc accaggtgca
    10921 gttgcgcgcc ggtgttggcg cacaggcgga tgacgttgcc ggtgttcggc gggatttccg
    10981 ggcgatgcag gatgacgtgg aacatcagga gtgcgcgctt accacggcag cggctcgccg
    11041 cgccagtcga ggaagctgcc ggactgcgac ggcgtggcgg catcgatcac ccgcagcaat
    11101 cccgccaccg cgtcgaccgg cgtgacctgc gcctgcgcgc cgcccatctc ggtctgcacc
    11161 caacccggat gcagcgccag taccacgatg ccgcgtccgg ccagcgcgtt cgccagcaac
    11221 gcgctggcca tgttctgcgc ggccttgctg atggcgtagc tgggcgtgcc gaagcgggcg
    11281 gtgtgggtga tcgagccgag ctgcgagctg atgttcgcga tgatggtgca tggcgatgcg
    11341 gcgtcgtcga ccgcgacggt gccatcgcgc agcaacggcg ccagcgcctg cgccagcagg
    11401 aacggcccga tcgcattcgt gcgcaggctg tcgtccagcg tggcctgctc gacatggccg
    11461 aagcgctcgc cggagtgcag cacgccggca ttgttgagca acaggtcgat gcggccgtcg
    11521 tcgcccagca ccagcggcag gtcgtggacc agcgaggcgc gcgatttcgc atcggcgacg
    11581 tccagcggca agacatgcag gcgggcggga tggtccccgg ccagcgcgtt cagcgcgctg
    11641 gccttgcccg ggtggcggca ggtggccacc acgtggtcgc cgcgcgccag caactggcgg
    11701 acgaattcca ggccgatgcc gcgattggcg ccggtgacca gggcggtgcg ggccatgcca
    11761 ggctcctcgg gtggcttccg gcctagtgta tcggccggcc ccgccggcta acatcggcgg
    11821 ttcgctgtcc cgcgtccgtc ctggagttgc ccatgtccgt gttccgcccc gccgtgcttg
    11881 ccgttgccct cgccaccgcc ctgtccgccg gcaccttcgc gccgcaggca cgcgccgcca
    11941 ccgccccggt ggtcgacatc gcctacgaca gcttcaccct gccgaatggc ctgcgggtga
    12001 tcgtgcacac cgaccgcaag gcgccgatcg tggccgtcaa cgtctggtac cacgtgggca
    12061 gcaaggacga accggccggc cgcagcggct tcgcccacct gttcgagcac ctgatgttca
    12121 acggctcgga gaacaacccg ggcgagttct tcggcccgtt caagcaggtc ggcgcgacca
    12181 accagaacgg cacgaccaat tccgaccgca ccaactactt cgagaacgtg ccgaccaccg
    12241 cgctggacat ggcgttgtgg atggaatccg accgcatggg ccacctgctc ggcgcgatcg
    12301 accagaagac cctggacgag cagcgcggcg tggtccagaa cgagaagcgc cagggccaga
    12361 accagcccta cggccaggtc tgggacgtgc tgggcaaggc catgtacccg gtgggccacc
    12421 catacaacca cagcaccatc ggctcgatga acgacctcaa cgcggcctcg ctggacgacg
    12481 tgaagacctg gttcaagacc tggtacggcc ccaacaacgc ggtgctggtg ctggccggcg
    12541 acatcgacct ggccaccgcg aaggagaagg tcgcgaagta cttcggcgac atcccggccg
    12601 gcccgaccat ggcccagccg aaggtggacg tggccgcgca caccaaggac agccgcggcg
    12661 agatgaccga caacgtcccg cagtcgcgga tctatcgcat gtggaacgtg gccgaatatg
    12721 gccagcccga cctggatcgc ctgcagctgt tcgcgcaggt gctgggcggc ggcaagtcgt
    12781 cgcgcctgga ccgtcgcctg gtgcaccagg acaagctggt cgacaacgtc ggcgcctcgg
    12841 cgttcggctc gcagctgggc agcaatttca tgatcaccgc cgcggtcaag cagggcgtgg
    12901 acgtggccaa ggtcgaggcg gccatcgacg aggagctgca gaagctgctc aaggacggcc
    12961 cgaccgcgga tgagctggcg cgcgcgcgca ccagcatcca ggccggctgg atccgcggcg
    13021 tggaacgcat cggcggcttc ggcggcaagg ccgacgcgct ggccgaatgc gccgtctata
    13081 ccggcgaccc gggttgcttc cgcgacagcc tgaagaccct gggcgacgcc accgtggcct
    13141 cggtgcgcgc caccggcaac acctggctgg gcgccggcaa gggcagccac accctggtgg
    13201 tgaacccggg caagcgcgtc gacatccccg aggagccggc ggtgaccccg gcaccgttca
    13261 agttgccggc cgtggacaag aagtacgcga ccaccgcgag cgcggtcgac cgcaaggccg
    13321 gcgtgccggt caccacgagc ttccccgacc tcaagttccc cagcctgcag cgcgccaccc
    13381 tgaagaacgg caccaaggtg gtcctggccg agcgccacga catcccggtg gtgcagatga
    13441 gctacgagtt ccgcggcggc cagtccgccg atccggcggc gaagctcggc accgccagct
    13501 tcgcgatggg cctgctggac gaaggcgcag gcgacctcga cgc
//
LOCUS       DAOOCX010000064        13529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_4191_length_13529_cov_8.420217, whole genome shotgun sequence.
ACCESSION   DAOOCX010000064 DAOOCX010000000
VERSION     DAOOCX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13529
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4191_length_13529_cov_8.420217"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PK399_07455"
     CDS             complement(<1..1020)
                     /locus_tag="PK399_07455"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPW12991.1"
                     /translation="MADLLWQKPGVAVDARIQAFLAGDDVLLDREFFLHDITASAAHA
                     EGLQRIGILTAEELDGLKRELVVLADDFRSGEFVLDECFEDGHSAIEARLTERLGDAG
                     RRIHTGRSRNDQILVATRLWLKEKLARVGELSREIARVALDRAGAEKELPIPGYTHIQ
                     RAVVSSAGMWWAGWAEAFIDDAIRAADTLKLVDANPLGTAAGYGVNLTLDREHTTQAL
                     GFARMQVSPVYAQLSRGKFELAALEALGSATLDLRRLAWDLSLYTTAEFGFVALPAQY
                     TTGSSIMPNKRNPDVVELMRATHASVAAARTEIEQLLSLPSGYQRDLQASKGAIVHGF
                     GRGLAA"
     gene            complement(1045..1992)
                     /gene="argC"
                     /locus_tag="PK399_07460"
     CDS             complement(1045..1992)
                     /gene="argC"
                     /locus_tag="PK399_07460"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPW12992.1"
                     /translation="MSALRVGIVGARGYVGGELIRLLAAHPRFELAYVTSRELVGQPL
                     AAHIAGYAGDVHYTSPAYEDLPQLAADAVVLALPNGKAAHIVQQFDAVGADPVIIDLS
                     ADHRFDPSWYYGLPELTRAQYAGQRRISNPGCYATAMQLAIAPMANVLDGPVQCFGVS
                     GYSGAGTTPSDKNDVELLRDNLMPYALTGHVHEREVSKHLGHPVEFLPHVAPHFRGLT
                     ITANLRLSIAFDIEEVFARYRGRYAGEPLVRVLDEAPWVSRIAGAHHVELGGFTLSGD
                     GKRLVVVATEDNLLKGAATQALQNLNMAFGLDEFLGIPL"
     gene            complement(1989..3326)
                     /locus_tag="PK399_07465"
     CDS             complement(1989..3326)
                     /locus_tag="PK399_07465"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPW12993.1"
                     /translation="MLTTNAIHDPQTRQTIVRLLSSMGSAKEINQYLKRFSQLDAARF
                     AIVKVGGAVLRDDLDALVSSLAFLQDVGLTPIVIHGAGPQLDAELSAAGIVKQTIEGL
                     RVTSPEALAIVRRVFHAQNLKLVEALQASDARATSIVSGVFEADYLDRERFGLVGEVK
                     RVDLAPIQASLQAGSIPVIASLGETVGGQILNVNADFAANELVQVLQPYKIVFLTGTG
                     GLLDADGKVIDSINLSTEYDHLMQQPWIEGGMRVKIEQIKDLLDGLPLASSVSITRPA
                     ELAKELFTHKGSGTLVRRGERVMEADSWDAFDLPRLRGLIESAFGRKLLPDYFETTKL
                     HHAYVSENYRAAVILTREDAGIYLDKFAVLDDAQGEGLGRAVWQVMRDANPRLFWRSR
                     HGNPVNPFYYAESDGCIKQEKWKVFWYGLSAMDEIARCIAYCASHAATLKDAA"
     gene            complement(3332..4426)
                     /locus_tag="PK399_07470"
     CDS             complement(3332..4426)
                     /locus_tag="PK399_07470"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HPW12994.1"
                     /translation="MLNAVLRHLEALVSFDTRNPPRLIGTGGIFDYVRAQLPDFRVDV
                     IDHGDGAVSLFAVRGNPRRVFNVHLDTVPSSEAWTADPHVMRRFDDRVVGLGVCDIKG
                     AAAGLIAAANVTEGDAAFLFSSDEEANDPRCIAAFLGSDHGFSEAIIAEPTRCEAVLA
                     HRGISSVLMKFKGEAGHASGANAMTASALHQAMRWGGNALDLVEGEANARFGGLTGLR
                     FNIGKVEGGIKANMIAPSAEVRFGFRPLPSQDIDGLHLRFGSCATAGALERYEETFRG
                     PSLPAGDVATAEERRLLARDLADELGLPIGNAVDFWTEASLFSQAGLTALVYGPGDIA
                     QAHTADEFVTLDQLQRYADSITRILGTTTP"
     gene            complement(4428..5648)
                     /locus_tag="PK399_07475"
     CDS             complement(4428..5648)
                     /locus_tag="PK399_07475"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPW12995.1"
                     /translation="MTSPNDSRDIVLAFSGGLDTSFCVPYLKEQGWNVHTVFADTGGV
                     DAEERAFIEARAAELGVASHVTVDGGPAIWNGFVKPFVWAGEGYQGQYPLLVSDRYLI
                     VDASLARAKELGTNAIAHGCTGMGNDQVRFDLAVKASGDYRIVAPIREIQKEHTQTRA
                     YEQAYLEARGFGVRSKQKQYTINENLLGLTMSGAEIDSWEAPGDGARGWCAKREHWPA
                     EALDVTLRFVGGEAVALDGNEMAGDKLLAKLNTMFAQYGVGRGMYTGDTTIGLKGRIV
                     FEAPGLTALLAAHRALEEAVLTKQQNRFKPEIARKWVELVYEGFYHDPLKTDLEAYLA
                     SSQAMVNGEVLLRTNGGRVDAVAVRSPHILNSKGATYAQSADWGVEEAEGFIKLFGMS
                     STLWAQVNGGQDAG"
     gene            complement(5717..6724)
                     /locus_tag="PK399_07480"
     CDS             complement(5717..6724)
                     /locus_tag="PK399_07480"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004427399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HPW12996.1"
                     /translation="MQPKHFLNTQDWSRADLDALLTQAALFKRNKLGSELKGKSIALV
                     FFNPSMRTRTSFELGAFQLGGHAVVLQPGKDAWPIEFDLGTVMDGDTEEHIAEVARVL
                     GRYVDLIGVRAFPKFVDWQRDREDIVLRSFAKYSPVPVINMETITHPCQELAHAMALQ
                     EHFGTQDLRGKKYVLTWTYHPKPLNTAVANSALTIATRMGMDVTLLCPTPDYVLDERY
                     MDWAARNVAESGGSLAVSHDIESAYRGADVVYAKSWGALPYFGNWEPEKPIRDQYKHF
                     MVDEAKMALTNNGVFSHCLPLRRNVKASDAVMDSPTCIAIDEAENRLHVQKAIMAALV
                     R"
     gene            complement(7000..7455)
                     /locus_tag="PK399_07485"
     CDS             complement(7000..7455)
                     /locus_tag="PK399_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW12997.1"
                     /translation="MTVPRALLTTLFVPALLAGALAQAQDPPPEVVIPPENVRFDYAQ
                     VLAVKPIYQVLRTTSQERVCDVSRQGESKLSRVVSSVKGRLSGNATPEERKAGLSNCR
                     MEAVSREFRRPIAYDVDYVYKGSKFRTRMARDPGNRLRVRVSITPQPHE"
     gene            7554..8945
                     /gene="cysS"
                     /locus_tag="PK399_07490"
     CDS             7554..8945
                     /gene="cysS"
                     /locus_tag="PK399_07490"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPW12998.1"
                     /translation="MTQSSSELHLYNTLSRRVEPFAPLDPASPTMYVCGPTVYNYVHI
                     GNARGPVVFGVLAALLRRRYGALRYARNITDVDDKINAAAKELGTPISAITDKYAAAY
                     REDMAMLGVDGDFAPDIEPAATAHMPQMIAMIERLIREGHAYAAEGHVLFAVASFDGY
                     GKLSRRDTDDMLAGARVEVAPYKRDPGDFVLWKPSTDDLPGWDSPWGRGRPGWHIECS
                     AMAEAHLGATIDIHAGGVDLQFPHHENEVAQSECAHGGAPFARWWLHNGMLNFGGAKM
                     AKSVGNIQRVHDLVREHPPEALRYALLSAQYRQPLEWSDALIEQSVRTLDRLYGTLRD
                     LADVDAQPAIPASIEAALDDDLNTPAALAEIARIAGEARKATSTEDRVALKSRLLGAG
                     LVLGLLQQSPAAWFERGSSSDDDARIQALIDERVEAKKSRDFARADAIRQQLADEGVL
                     LEDTVAGVRWKRA"
     gene            8942..9400
                     /locus_tag="PK399_07495"
     CDS             8942..9400
                     /locus_tag="PK399_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HPW12999.1"
                     /translation="MSDSPFPLEGTPAEAQAAIAEEFAFFGDWSERYQYLIDLGRKLP
                     PFPDAWKTEEHRLLGCQSMVWIVPSGDASKLEFAAASDSAIVSGLVFLALRVYSGRSA
                     GEILATDPDYIAAIGLAKHLSPTRSNGLAALLAFIREHARHMQAGANLAG"
     gene            9397..10626
                     /locus_tag="PK399_07500"
     CDS             9397..10626
                     /locus_tag="PK399_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004427407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW13000.1"
                     /translation="MSALPMSPLASIGFRWLMLYRICTLFSYQIVAVTVGWHVYELTR
                     DPWSLGLIGLAEVIPFFCVAPFSGYLVDHLPRRKLGAAACAVLALNALVLALVAGDVI
                     PLRGLWPIYLVIAIGGVARSFLGPVHNALFARVLRRDQFGRGASIGSVVFQAGLVLGP
                     AIGGVLVGAAGKWAAYGLSTVFALVAAGALLCMPVSEPAQQQQRGPVFASIAEGLRFV
                     FGHQILLAALALDMFAVLFGGAISLAPAFIKDVLHAGPEALGILRGAPALGSVAAALW
                     LARHPLQANAGRIMLVAVACFGACMIGFGLSTAFWLSVLFLLLSGVFDGVSVVLRSTV
                     LQLSTPDEMRGRVSSVNSIFISSSNELGAFYAGSMARLLGLVPSVVLGGFVTMGVVGI
                     TAWKAPRLRKLDLRELQ"
     gene            complement(10710..10910)
                     /locus_tag="PK399_07505"
     CDS             complement(10710..10910)
                     /locus_tag="PK399_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HPW13001.1"
                     /translation="MNKDIIAGNWKQLKGKAQAKWGDLTDDVFDVAEGNSEYLSGKLQ
                     EKYGWQRERADKEVSDFRNTLN"
     gene            complement(11127..12119)
                     /gene="dksA"
                     /locus_tag="PK399_07510"
     CDS             complement(11127..12119)
                     /gene="dksA"
                     /locus_tag="PK399_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HPW13002.1"
                     /translation="MAAKKPVKKAAKPAKKAVPAKKPAPAKKPAAKPVAKKAAPAKKP
                     APAKKPAAKPVAKKAAPVKKPVPAKKPAAKTATPAKKPAPAPVKKAAPAAAAKKAPVA
                     KSTPAKAAPVKAVAAKPAPAPAKAAPAKTAPAPSKAAPAPKKDAVQVAAPKPAAVVRK
                     PQGKVAVAVVSRPASPAPKGKLRVVPYQTDEATGRPILPVGYKPAADEEYMSPLQLEY
                     FRQRLFNWRNDLVEESKQTIENLKEEVRDVGDEAERATRETENSLELRTRDRYRKLIG
                     KIDSTLKRLDGGDYGFCVDTGEEIGLDRMEARLTAERTIDAQERWEHMQKQMGD"
     gene            12228..12458
                     /gene="yidD"
                     /locus_tag="PK399_07515"
     CDS             12228..12458
                     /gene="yidD"
                     /locus_tag="PK399_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPW13003.1"
                     /translation="MIEHFLIALLRGYKRWISPLLGTRCRFVPTCSEYAMEAIGRFGA
                     LKGSWLAIRRIGRCHPLHPGGHDPVPPPKEPT"
     gene            12455..>13529
                     /locus_tag="PK399_07520"
     CDS             12455..>13529
                     /locus_tag="PK399_07520"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPW13004.1"
                     /translation="MTSTLIVNARLVNEGREFDGDLRFADGRIEQIGNGLAARAGETV
                     IDAAGRRLLPGMIDDQVHFREPGMEYKADMAIESAAAVAGGLTSFMDMPNTVPPTLDA
                     AALEDKYRRAAGRCHANYGFYMGASNDNLEAVPAIDPRATPGLKVFMGASTGNMLVDN
                     PETLDAIFRDTPVPIITHCEDTPTIDATLAQYKAKYGDDIPVECHPDIRSREACIKST
                     RLALALARRHGTQLHVLHISTADELALFEQGPLIRADGSRKQITAETCVHFTRFARGD
                     YARLGNLIKCNPAIKDEADREAIIDALAGDILDVLATDHAPHTLEEKGRPYASAPSGL
                     PLVQFALNAALELVHEGRLTTAQV"
BASE COUNT         2105 a         4614 c         4459 g         2351 t
ORIGIN      
        1 cgccgcaagg ccgcggccga agccgtggac gatcgcgccc ttgctggcct gcaggtcgcg
       61 ctggtaaccg gatggcagcg acagcagctg ctcgatctcg gtgcgcgccg cggccacgct
      121 ggcatgggtc gcgcgcatca gctcgacgac atccggattg cgcttgttcg gcatgatcga
      181 actgccggtg gtgtactgcg ccggcaacgc cacgaagccg aactcggcgg tggtgtacag
      241 cgacaggtcc cacgccaggc gtcgaaggtc cagcgtggcg ctgcccagcg cttccagcgc
      301 ggccagctcg aacttcccgc gcgacagctg cgcgtagacc ggcgacacct gcatccgcgc
      361 gaacccgagt gcttgcgtgg tgtgctcgcg atccagggtg aggttcacgc catagccagc
      421 ggccgtcccc aatggattcg catcgacgag cttgagcgta tcggcagcgc ggatcgcatc
      481 gtcgatgaag gcctcggccc agcccgccca ccacatcccc gccgacgaca ccaccgcacg
      541 ctggatatgc gtgtaaccgg ggatcggcaa ctctttctcg gctccggcgc ggtccagcgc
      601 gaccctcgcg atctcgcgcg aaagctcgcc aacgcgcgcc agcttttcct tcagccacag
      661 gcgcgtcgcc accaggatct ggtcgttgcg gctgcggccg gtgtggatcc tgcggccggc
      721 atcgcccaag cgctcggtca agcgcgcctc gatcgcggaa tgcccatcct cgaagcactc
      781 gtccaggacg aactcgccac tgcggaaatc gtcggccagc accacaagtt cgcgcttcag
      841 tccgtcgagc tcctccgcag tgaggatgcc gatgcgctgc aagccctcgg catgcgccgc
      901 actggcggtg atgtcgtgca ggaagaattc acgatccagc agcacgtcgt cgccggccag
      961 gaacgcctgg atccgcgcat cgacggcgac gccgggtttt tgccacaaca agtcagccat
     1021 cgtcgccctt cccgtgttgg aggattacag cggaatcccg aggaattcgt ccaaaccaaa
     1081 tgccatgttg agattctgca gcgcctgcgt ggccgcgcct ttcaacaggt tgtcctcggt
     1141 ggcgaccacg accagccgct tgccgtcgcc actcagggtg aagccaccga gttcgacgtg
     1201 gtgagcgccc gcgatgcggc tgacccacgg cgcttcgtcc agcacgcgca ccagcggctc
     1261 gccggcatag cggccgcggt agcgggcgaa gacttcctcg atgtcgaacg cgatggacag
     1321 ccgcaggttc gcggtgatcg tcagcccgcg gaaatgcggc gccacgtgcg gcaggaattc
     1381 gaccgggtgg cccaggtgct tcgagacttc gcgctcatgc acgtgcccgg tcagcgcata
     1441 cggcatcagg ttgtcacgca gcagttcgac gtcgttcttg tcgctcggcg tggtgcccgc
     1501 gccggaatac ccgctcacgc cgaagcactg caccggccca tccaggacgt tcgccatcgg
     1561 cgcgatcgcc aactgcatcg cggtggcgta gcagccggga ttgctgatgc ggcgctggcc
     1621 ggcgtactgc gcgcgggtca gttcggggag gccgtagtac caggacggat cgaagcgatg
     1681 gtcggcggac aggtcgatga tcacgggatc ggcgccgacg gcatcgaatt gctggacgat
     1741 gtgcgcggcc ttgccgttcg gcagcgccag cacgaccgca tcggcagcca gttgcggcag
     1801 gtcttcgtac gcgggcgagg tgtaatgcac atcgcccgca tagcccgcga tgtgcgcggc
     1861 cagcggctgc ccgaccagtt cgcgcgaggt cacgtaggca agctcgaaac gcggatgcgc
     1921 ggccagcaag cggatcagct cgccgccgac gtagccacgg gcaccgacga tgccgacgcg
     1981 caaggcgctc atgccgcgtc cttcagggtg gccgcatggc tcgcgcagta cgcgatgcat
     2041 cgcgcgatct cgtccatcgc cgacagcccg taccagaaca ccttccactt ctcctgcttg
     2101 atgcagccgt cggattccgc gtaatagaac ggattgaccg gatttccatg gcgcgaacgc
     2161 cagaacaggc gtggattcgc atcacgcatg acctgccaga ccgcgcgacc cagcccttcg
     2221 ccctgcgcgt catccaggac cgcgaacttg tcgaggtaga tgccggcgtc ttcgcgggtg
     2281 aggatcaccg cggcgcggta gttctcgctg acatacgcgt gatgcagttt cgtggtctcg
     2341 aagtaatccg gcagcagctt gcggccgaac gcggactcga tcagcccgcg caggcgcggc
     2401 aggtcgaagg catcccagga gtccgcttcc atgacccgct cgccgcgccg caccagggtg
     2461 cccgagccct tgtgggtgaa cagttccttg gccagttccg ccggccgggt gatcgacacc
     2521 gacgacgcca gcggcaaacc gtccagcagg tccttgatct gctcgatctt gacccgcatg
     2581 ccgccttcga tccacggctg ctgcatcagg tggtcgtatt cggtggacag gttgatcgag
     2641 tcgatcacct tgccgtccgc gtccagcagg ccaccggtgc cggtgaggaa cacgatcttg
     2701 tacggctgca gcacctgcac cagttcgttg gcggcgaagt cggcattgac gttgaggatc
     2761 tgcccgccga cggtttcgcc cagcgaggcg atcaccggga tcgagccggc ctgcaggctg
     2821 gcctggatcg gagcaaggtc cacgcgtttc acctcaccga ccaggccgaa gcgctcacgg
     2881 tcgaggtagt cggcttcgaa caccccggag acgatgctgg tggcgcgcgc atcacttgcc
     2941 tgcagcgctt cgaccagctt caggttctgc gcatggaaca cccggcgcac gatcgccagc
     3001 gcttccggcg aggtcacccg cagcccctcg atggtctgct tgacgatgcc ggcggcggac
     3061 agctccgcgt cgagctgcgg acccgcgccg tggatgacga tcggggtcag ccccacgtcc
     3121 tgcaggaagg ccagcgacga caccagcgcg tcgaggtcgt cgcgcagtac cgcgccgccc
     3181 accttgacga tcgcgaagcg tgccgcgtcc agctgcgaaa agcgcttgag gtactggttg
     3241 atttccttgg cgctgcccat gctcgagagc aggcgcacga tggtctggcg ggtctgcggg
     3301 tcgtggatgg cattcgtggt caacatcggc gtcacggcgt ggtggttcca aggattcggg
     3361 taatggaatc ggcgtaacgc tgcaactggt cgagcgtgac gaattcgtcg gcggtatggg
     3421 cctgggcgat gtcgcccggc ccgtagacca gtgcggtcag gccggcctgc gagaacagcg
     3481 acgcctcggt ccagaaatcg accgcgttgc cgatcggcag gccgagctcg tcggcgaggt
     3541 cgcgcgccag caggcggcgt tcctccgcag tcgccacgtc gccggccggc aacgacgggc
     3601 cgcggaaggt ttcctcgtag cgttccagcg cgcctgcggt cgcgcaggaa ccgaaacgca
     3661 ggtgcaaccc atcgatgtcc tgcgacggca gcgggcggaa cccgaagcgc acctcggcgc
     3721 tcggcgcgat catgttggcc ttgatcccgc cttccacctt gccgatgttg aagcggagcc
     3781 cggtcaggcc accgaagcgc gcgttcgctt cgccttcgac caggtccagc gcgttgccgc
     3841 cccagcgcat cgcctggtgc agcgcactcg cggtcatcgc gttcgcaccg gaggcatgcc
     3901 cggcctcgcc cttgaacttc atcagcaccg agctgatccc gcgatgcgcc agcaccgcct
     3961 cgcaacgcgt cggttcggcg atgatcgctt cggaaaatcc gtggtcgctg cccaggaacg
     4021 cggcgatgca gcgcggatcg ttggcttcct cgtcggagga aaacaggaac gcggcatcgc
     4081 cctcggtcac gttcgccgcc gcgatgaggc ccgccgccgc gcccttgatg tcgcacacgc
     4141 ccaggccgac cacgcgatca tcgaagcggc gcatgacatg cggatccgcg gtccacgcct
     4201 ccgacgatgg cacggtgtcc aggtgcacgt tgaacacgcg gcgcgggttg ccgcgcacgg
     4261 cgaacagcga caccgcgccg tcgccgtggt cgatcacatc gacacggaaa tccggcagtt
     4321 gcgcgcggac gtagtcgaag atcccgccgg tgccgatcaa gcgcggcggg ttgcgggtgt
     4381 cgaaggacac caacgcttca aggtggcgaa ggaccgcgtt gagcatgtca gccggcgtcc
     4441 tgcccgccat tgacctgcgc ccacaacgtg ctgctcatgc cgaacagttt gatgaagcct
     4501 tcggcctcct cgacgcccca gtctgcggac tgcgcgtagg tcgcaccctt cgaattgagg
     4561 atgtgcggtg aacgcaccgc cactgcatcc acacgaccgc cgttcgtgcg caggagcact
     4621 tcgccgttga ccatcgcctg cgacgaggcc aggtaggctt ccaggtcggt cttcaggggg
     4681 tcgtggtaga agccctcgta caccagctcc acccactttc gcgcgatctc cggcttgaag
     4741 cggttctgct gcttggtcag caccgcttct tccagcgcgc gatgggcggc aagcaacgcg
     4801 gtcagccccg gcgcctcgaa cacgatccgt cccttcaggc cgatggtggt gtcgccggtg
     4861 tacatgccgc gacccacgcc gtattgcgcg aacatggtgt tgagcttggc aaggagtttg
     4921 tcgccagcca tttcgttgcc atcgagcgcc acggcttcgc cgccgacgaa gcgcagggtc
     4981 acgtccagcg cttcggcggg ccaatgctcg cgcttcgcgc accagccacg cgcgccatcg
     5041 cccggcgctt cccagctgtc gatctcggca ccggacatgg tcaggcccag caggttctcg
     5101 ttgatggtgt actgcttctg cttgctgcgc acgccgaagc cgcgcgcctc caggtaggcc
     5161 tgctcgtagg cgcgcgtctg cgtgtgttcc ttctggatct cgcggatcgg cgccacgatg
     5221 cggtagtcgc cgctcgcctt gaccgccagg tcgaagcgca cctggtcgtt gcccatgccg
     5281 gtgcagccgt gggcgatggc attggtgccc agttccttcg cccgcgccag cgacgcatcg
     5341 acgatcaggt agcggtccga caccagcaac ggatactggc cctggtagcc ctcgcccgcc
     5401 catacgaacg gcttgacgaa gccattccag atcgccggcc cgccatccac ggtcacgtgc
     5461 gatgccacgc ccagctcggc cgcgcgcgct tcgatgaagg cgcgttcctc ggcatccacg
     5521 ccgccggtat cggcgaacac ggtatgcacg ttccagccct gctctttcag gtagggcacg
     5581 cagaaactgg tatccagccc gccggaaaag gccaggacga tgtcgcggga atcattgggg
     5641 gaggtcatgt cgctgtctcg ctggttgacg ccctcgcccg ctcgcgggaa agggtgaagg
     5701 tgaaggtggg aaaggctcaa cgcaccagcg cggccatgat cgccttctgc acatgcaggc
     5761 ggttctcggc ttcgtcgatg gcgatgcagg tgggtgaatc catcaccgcg tcgctggcct
     5821 tcacgttgcg gcgcaacggc aggcagtggc tgaagacgcc gttgttggtc agcgccatct
     5881 tggcttcgtc gaccatgaag tgcttgtact ggtcgcggat cggcttctcg ggttcccagt
     5941 tgccgaagta cggcagcgcg ccccagctct tggcgtagac cacgtcagcg ccgcggtagg
     6001 cgctttcgat gtcgtggctc accgccagcg aaccgccgct ctcggccacg ttccgtgctg
     6061 cccagtccat gtaacgctcg tcgagcacgt agtccggcgt ggggcacagc agggtcacgt
     6121 ccatgcccat gcgcgtcgcg atggtcagcg cggaattggc cactgcggtg ttcagcggct
     6181 tgggatggta ggtccaggtc agcacgtact tcttgccgcg caggtcctgc gtgccgaagt
     6241 gctcctgcaa ggccatcgcg tgcgccagct cctggcaggg atgggtgatc gtctccatgt
     6301 tgatcaccgg caccggcgaa tacttcgcga acgacctcag cacgatgtcc tcgcggtcgc
     6361 gctgccagtc gacgaacttg gggaacgcgc gcacgccgat caggtcgacg tagcggccca
     6421 gcacccgcgc cacttcggcg atgtgctcct cggtgtcgcc gtccatcacc gtgcccaggt
     6481 cgaactcgat cggccaggca tccttgccgg gctgcaacac caccgcatgg ccgcccagtt
     6541 ggaacgcgcc caactcgaag ctggtgcgcg tgcgcatcga cggattgaag aacaccaggg
     6601 cgatcgactt gcctttcaac tcgctgccga gcttgttgcg cttgaacaag gctgcctggg
     6661 tcagcagggc gtcgaggtcg gcccggctcc agtcctgggt gttgaggaaa tgcttgggct
     6721 gcatcatcgg ttccttggat cggggcggtg gcgaggggac ggttgcgctt gaaaccagag
     6781 gccgaaaacg aaaaaaccca gcctgagggc tgggtcttga cgggagatgc acgacaacgc
     6841 gatccggtct acccagcggt tcgggaaagt tccggtcggc gcgcacgcga ggtcatgccg
     6901 gaggccatcc gggcagagtt ggcatagcgc gtggcgaggg atgcgtgcat cagggcgcgg
     6961 atgctgacat gccgcgtcgc agcatgcaag cctcgtgcct cattcgtgcg gctgcggggt
     7021 gatcgagacg cgcacgcgca ggcggttgcc cgggtcgcgc gccatccgcg tccggaactt
     7081 gctgcccttg tagacgtagt ccacgtcgta ggcgatcggt cggcggaact cgcgcgacac
     7141 cgcctccatg cggcagttgc tcaggcccgc cttgcgctct tcgggtgtcg cgttgccgct
     7201 cagacggccc ttcaccgaac tcaccacccg cgacaatttc gactcgccct gccggctcac
     7261 gtcgcagacg cgttcctggc tggtggtgcg caggacctgg tagatcggct tcaccgcaag
     7321 cacctgtgcg tagtcgaagc gcacgttctc cggcgggatc acgacctcgg gcggcggatc
     7381 ctgcgcctgc gccagcgcgc cagccaacaa ggcaggcacg aacagggtgg tcaggagggc
     7441 gcgggggaca gtcatgggcc gcaagtctat cggcgcaggg cgcggcgggt atgaatcgcc
     7501 gctgtcgtcg gcacgctggc cgctacaatg ggcggtccca cgcgcgtacc gccatgaccc
     7561 agtcttcaag cgagctccac ctctacaaca cgctgtcgcg acgcgtcgaa cccttcgcgc
     7621 cgctcgaccc cgccagcccg accatgtacg tctgcgggcc cacggtctac aactacgtgc
     7681 acatcggcaa cgcccgcggg ccggtggtgt tcggggtgct ggccgccctg ctgcgtcgcc
     7741 gctacggcgc cctgcgctac gcgcgcaaca tcaccgacgt cgacgacaag atcaatgcgg
     7801 cggcgaagga actgggcacg cccatttccg cgatcacgga caagtatgcg gccgcctatc
     7861 gcgaggacat ggcgatgctg ggcgtggacg gcgacttcgc ccccgacatc gaaccggccg
     7921 cgaccgcgca catgccgcag atgatcgcga tgatcgagcg cctcatccgc gagggccatg
     7981 cgtatgcggc cgaaggccat gtgctgttcg cggtggccag cttcgacggc tacggcaagc
     8041 tctcgcgccg cgacaccgac gacatgctgg ccggcgcacg cgtggaagtc gcgccgtaca
     8101 agcgcgatcc cggcgacttc gtgctgtgga aaccctcgac cgacgacctg cccggctggg
     8161 attcgccctg gggccgcggc cgccctggct ggcacatcga atgctcggcg atggcggagg
     8221 cgcacctcgg cgcgaccatc gacatccacg ccggcggcgt cgacctgcag ttcccgcacc
     8281 acgagaacga agtggcgcag agcgaatgcg cgcacggcgg cgcacccttc gcccgctggt
     8341 ggctgcacaa cggcatgctc aatttcggcg gcgccaagat ggcgaagtcg gtgggcaaca
     8401 tccagcgcgt ccacgacctg gtccgcgaac atccgccgga agcgctgcgc tacgcgctgc
     8461 tgtccgcgca gtaccggcag ccactggaat ggtcggatgc gctgatcgag cagtcggtgc
     8521 gcacgctgga ccggctgtac ggtacgttgc gcgacctggc cgatgtcgat gcgcagcctg
     8581 cgattcccgc gagcatcgag gccgcgctgg acgacgacct caacacgccc gccgcgttgg
     8641 ccgagatcgc ccgcatcgcc ggtgaagcgc gcaaggcgac atcgaccgaa gaccgggtcg
     8701 cgctgaagtc gcgattgctg ggggcgggcc tggtgctggg gttgctccag cagtcgcctg
     8761 ccgcatggtt cgaacgcggg tcatcgagcg acgacgacgc gcgcatccag gcattgatcg
     8821 acgagcgcgt cgaggcgaag aaatcccgcg acttcgcccg cgccgacgcc atccgccagc
     8881 agctcgccga cgagggcgtc ctgctggagg acaccgtggc aggtgttcgc tggaagcgcg
     8941 catgagcgac tcgccgttcc cgctcgaagg cacgccggcc gaggcgcagg cggccatcgc
     9001 cgaagagttc gcgttcttcg gcgactggtc ggagcgctac cagtacctga tcgacctcgg
     9061 tcgcaagctg ccgcctttcc ccgacgcgtg gaagaccgaa gagcaccgcc tgctgggctg
     9121 ccagtcgatg gtctggatcg tgccgtcggg tgatgcctcg aagctcgaat tcgccgccgc
     9181 cagcgattcc gccatcgtgt cgggcctggt gttcctggcc ttgcgcgtct attccggccg
     9241 cagtgccggg gagatcctgg ccaccgatcc ggactacatc gccgcgatcg gcctggccaa
     9301 gcacctgtcg ccgacgcgca gcaatggcct ggcggcgctg ctggccttca tccgcgaaca
     9361 tgccaggcac atgcaggccg gggccaacct ggccggatga gcgcgctgcc gatgtcgccg
     9421 ctggccagca tcggtttccg ctggctgatg ctgtatcgca tctgcacgct gttctcgtac
     9481 cagatcgtgg cggtgaccgt gggctggcat gtctacgaac tcacccgcga tccgtggtcg
     9541 ctggggctga tcggcctggc cgaggtcatc ccgttcttct gcgtggcgcc gttttcaggc
     9601 tacctggtcg accacctgcc tcgacgcaag ctcggcgcgg cggcgtgtgc ggtgctggcc
     9661 ttgaatgcgc tcgtgctggc gctggtcgcc ggcgacgtca tcccgctccg cggcctgtgg
     9721 ccgatctacc tggtgatcgc catcggtggg gtcgcgcgtt cgttcctggg cccggtgcac
     9781 aacgccctgt tcgcgcgggt gctgcgccgc gaccagttcg ggcgcggtgc cagcatcggc
     9841 agcgtggtgt tccaggccgg tctggtactc ggccctgcga ttggcggcgt cctggtcggt
     9901 gccgccggca aatgggcggc gtacgggctg tcgaccgtgt tcgcgctggt cgccgcagga
     9961 gcgctgctgt gcatgccggt gagcgaaccc gcccagcagc agcaacgtgg cccggtgttc
    10021 gccagtatcg ccgaggggct gcgcttcgtg ttcggccacc agatcctgct ggccgcgctg
    10081 gcgctggaca tgttcgcggt cctgttcggt ggcgcgatct cgctggcccc ggccttcatc
    10141 aaggacgtgc tgcacgcagg gccggaggcg ctgggcatcc tgcgtggcgc gcccgcgctc
    10201 gggtcggtcg ccgccgcgtt gtggctggcg cggcatcccc tgcaggcgaa tgccggccgg
    10261 atcatgctgg tggcggtggc ctgcttcggc gcctgcatga tcggcttcgg cctgagcacg
    10321 gccttctggc tgtcggtgct cttcctgctg ctgtcgggcg tgttcgatgg cgtgtcggtg
    10381 gtgctgcgct cgaccgtcct gcagctcagt acgccggacg agatgcgtgg acgcgtatcc
    10441 tcggtcaaca gcatcttcat cagttcgtcg aacgaactgg gcgcgttcta tgccggatcg
    10501 atggcgcgac tgctggggct ggtgccgtcg gtggtgctgg gcggcttcgt caccatgggc
    10561 gtggtcggca tcaccgcatg gaaggcgccg cggctgcgca agctggacct gcgcgaactg
    10621 cagtgagcca cctccgtcgt tgcgcacacg aagaaagccc ggcgcatgcg ccgggctttc
    10681 gcttcaacgg atggctgatt ccgctggaat cagttcagcg tgttgcggaa atcggagact
    10741 tccttgtcgg cgcgctcgcg ctgccagccg tatttttcct gcaacttgcc ggagaggtat
    10801 tcgctgttgc cttcggcgac gtcgaacacg tcatcggtga ggtcgcccca cttcgcctgg
    10861 gccttgccct tcagctgctt ccagttgccg gcgatgatgt ccttgttcat gtgcagtgct
    10921 ccttctgggg gttgggtggc atccatcgat ccagacgacg cattcccgtc cgtcgttgtt
    10981 gccacccgca caggttgcac gtggccacgt agtcgccggg tgcaggtttc gttagcagcc
    11041 actcacgaac gccaacgcac gttcagccaa aaccgaaaac ggcgccctgc ggcgccgttt
    11101 tcgctcagtg cgacaggcac gaaccgtcaa tcgcccatct gcttctgcat gtgttcccag
    11161 cgttcctgcg catcgatggt gcgctcggcg gtgagccgcg cctccatccg atccaggccg
    11221 atctcctcgc cggtatccac gcagaacccg tagtccccgc catccagccg cttcagcgtg
    11281 ctgtcgatct tgccgatcag cttgcgatag cggtcgcggg tgcgcagttc cagcgagttc
    11341 tcggtttcgc gggtggcgcg ctcggcttca tcgccgacat cgcgcacttc ttccttgagg
    11401 ttctcgatgg tctgcttgga ttcctcgacc aggtcgttgc gccagttgaa caggcgctgg
    11461 cggaaatact cgagctgcaa cgggctcatg tattcctcgt cggcggccgg cttgtagccc
    11521 accggcagga tcggacggcc ggtggcctcg tcggtctggt acggcacgac cctcagcttg
    11581 cctttcggcg cgggactggc gggccgggac accaccgcca cggcgacctt gccctgcggc
    11641 ttgcgcacga ccgcagccgg cttcggcgcc gcaacctgca ccgcgtcttt cttcggtgcc
    11701 ggagccgcct tggatggggc gggtgccgtc ttggccggcg cagcctttgc tggtgcgggc
    11761 gcgggcttgg ctgccactgc cttcacggga gcggccttcg ccggggtgga cttggccacc
    11821 ggtgctttct tggcggctgc ggccggagcc gctttcttca ccggagcggg agccggcttc
    11881 ttggccgggg tcgccgtctt ggcagccggt ttcttggcag gcaccggctt tttcaccggg
    11941 gccgccttct tcgccaccgg cttggccgcc ggcttcttgg cgggtgccgg cttcttcgcc
    12001 ggagcggctt tcttggccac gggcttggcg gcgggcttct tggcgggcgc cggcttcttc
    12061 gccggaacgg ccttcttggc cggcttggcg gccttcttca cgggtttttt cgctgccacg
    12121 agcaggtatt cctcgatgtt cccttgaggc gggaaagcgc gctgttatac cctgatgggc
    12181 acatcgcagc aaccgcgcaa cgatgcgcca cgctgcgttc aaggcccgtg atcgaacatt
    12241 ttctcatcgc gctgttgcgc ggctacaagc gctggatcag ccccctgctc ggcacccgct
    12301 gccggttcgt gcccacctgt tcggagtacg cgatggaggc catcggccgg ttcggcgcac
    12361 tcaagggcag ctggctggcg atccgtcgca tcggccgctg ccatcccctg caccccggcg
    12421 gacacgaccc cgtcccgccc cccaaggaac caacatgact tcgacgctga tcgtcaatgc
    12481 ccggctggtc aacgaaggcc gcgagttcga tggcgacctg cgcttcgcgg acggccgcat
    12541 cgagcagatc ggcaacggac tggccgcgcg ggccggcgag accgtgatcg atgccgccgg
    12601 ccgccgcctg ctgcccggca tgatcgatga ccaggtccat ttccgcgaac cgggcatgga
    12661 atacaaggcg gacatggcga tcgagtcggc cgccgcggtc gccggcgggc tgaccagctt
    12721 catggacatg cccaataccg tgccgccgac cctggacgcg gctgcgctgg aggacaaata
    12781 ccgtcgcgcc gccggccgct gccacgcgaa ctacggcttc tacatgggcg cgagcaacga
    12841 caacctggag gcagtccccg ccatcgaccc gcgcgccacg cccgggctga aggtcttcat
    12901 gggcgcctct accggcaaca tgctggtcga caacccggag acgctggacg cgatcttccg
    12961 cgacaccccc gtcccgatca tcacccactg cgaagacacc ccgaccatcg acgccaccct
    13021 cgcccagtac aaggcgaagt acggcgacga catcccggtg gaatgccacc cggacatccg
    13081 cagccgcgag gcctgcatca agtccacgcg cctagcactg gcgctagcgc gcaggcacgg
    13141 cacgcaactg cacgtgctgc acatcagcac cgccgacgaa ctggcgctgt tcgagcaggg
    13201 cccgctgatc cgcgccgatg gcagccgcaa gcagatcacc gccgagacct gcgtgcactt
    13261 cacccgcttc gcccgcggcg actacgcgcg gctgggcaac ctgatcaagt gcaaccccgc
    13321 aatcaaggac gaggccgacc gcgaggcgat catcgacgca ctggccggcg acatcctgga
    13381 tgtgctggcc accgaccatg ctccgcacac gctggaggaa aagggccggc cgtacgccag
    13441 cgcacccagt ggcctgccgc tggtgcagtt cgctctcaat gccgccctgg aactggtcca
    13501 cgagggccgc ctgaccaccg cgcaagtgg
//
LOCUS       DAOOCX010000065        13415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_4237_length_13415_cov_7.557335, whole genome shotgun sequence.
ACCESSION   DAOOCX010000065 DAOOCX010000000
VERSION     DAOOCX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 13415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13415
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4237_length_13415_cov_7.557335"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="PK399_07525"
     CDS             complement(<1..1409)
                     /locus_tag="PK399_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HPW13005.1"
                     /translation="MSTIAQDAPAGPPAAASPAAFRPPNVALTTLGLALASFMQVLDT
                     TIANVSLPTISGNLGGSANQATWVITSFAVSTAIALPLTGWLARRFGERKLFVWSTMA
                     FVIASFLCGIANSMGMLVVARALQGFVAGPMYPITQALLLSIYPPAKRGQAIALLAMV
                     TVVAPIAGPILGGWITDNYSWEWIFFVNVPIGIFASLVVGRQLKGRPERLETPKMDYI
                     GLATLVLGVGALQILLDLGNDEDWFASQTIVVLAIIAAISLAVFVIWELTDKDPIVNL
                     RLFRHRNFAAGTLAMVVAYSAFFSVGILVPLWLQRNLGYTAIWAGFATAPIGILPVLL
                     TPFVGKYANRFDLRMLASIAFIAMSFTSFARSHFNLDVNFEHVAWIQLFQGVGVALFF
                     MPVLQILLSDLEPHEIAAGSGLATFVRTLGGSFAASLTTYAWSERGAVHHAHLTEKIS
                     TQDPATLQSVMQLGGGDLQR"
     gene            complement(1538..2755)
                     /locus_tag="PK399_07530"
     CDS             complement(1538..2755)
                     /locus_tag="PK399_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW13006.1"
                     /translation="MTTETTPNTSTGNAAPLPPSPPARAGNGKRKRALLILLGVVLIA
                     GGAWLAWYLTVARWHQSTDDAYVQGNIVSITPQTPGTVVAINAEDGMRVEAGQVLVQL
                     DPNDAQVAYAQAEANLANTVRQVRGLYSSVDAAQADIAARQVAVAQARADVARRSGLV
                     ATGAVSSEELAHARAQLDAAEAALSGSQGQLSRNRALVDATTVGNQPQVQAAAAQLRQ
                     AYLNLQRAAIVAPISGFVGKRAVQLGQRVQPGAALMTVVPLEQVWVEANFKETQLHSM
                     RLGQPVELTADIYGDDVTYTGKITSLGLGTGSAFALLPAQNASGNWIKIVQRVPVRIE
                     LDPKQVAEHPLRLGMSMSADVGIRDQGGKTLPVAAPAKPVLTTQAYAKQLQDADARID
                     TIISQNLAQGRRS"
     gene            complement(2763..4211)
                     /locus_tag="PK399_07535"
     CDS             complement(2763..4211)
                     /locus_tag="PK399_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPW13007.1"
                     /translation="MSRPSLLATALATALLATACASSGDIAPRGAMRDAGSLQSSASL
                     AGTRVSDAAWPSERWWTAFGDPQLDALVDEALANAPSIEAADARTRKAIAQAGLADAA
                     RKPTVGAGAQVLGAQLPPALVGDEIGGDFKVVNVLTLSFAWNPDVWGVSRSKWQAAVG
                     SARATEVDAQSARLALAANVARSYVGLARAFDALDAANAEAARADGLISLNQQRIKAG
                     LDNTIALNQNRSAAAAARQQAQAAQHDIDAMRNALAALLGAGPDRGLEIARPMLKTPG
                     IALPSVLPSDLLARRADIVAARWRVEAAQHGIDASKAAFYPSINLSALAGLAAGNLGD
                     LFGSDALLLNGGPALSLPIFEGGRLEQQLRSSDADRDLAVANYNETLVGAIRDVANAV
                     QAARALDAQVASTTVARDAAARAHALVLQRQRAGLATQLDVLAAQAPLLRLDQQLAAL
                     VAARRIASIDLDQALGGGIAVDSPNPTASHSN"
     gene            complement(4215..4697)
                     /locus_tag="PK399_07540"
     CDS             complement(4215..4697)
                     /locus_tag="PK399_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPW13008.1"
                     /translation="MTNPHSPDHPVAPAIGSCSGSTLGLLFRQARDAMWARMEVELAR
                     CGHELNFSQFITIKTLANSTTSVTELARAAYLHPGAMTRLLDKLEARGILERVADPND
                     RRALHIHLTDAGKAIWRDIDQCGQRVREAALQGLEPVERAQLLALLERVRDNLNSPTD
                     "
     gene            complement(4780..5571)
                     /gene="ccsA"
                     /locus_tag="PK399_07545"
     CDS             complement(4780..5571)
                     /gene="ccsA"
                     /locus_tag="PK399_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPW13009.1"
                     /translation="MTIVLIAVVIYVAAAALLWRSATGDAGPRRGWIPVAVLAVLFHA
                     ETHAEAWRLLQGADMHFFAALSLVGLGMAALTTAFGASGRMPALGIVVFPLAAASLLA
                     YRAYGHADNASGLGWRLQLHAWMALLAYATLALSAVLAIFLWAQERALRRREFRGWLR
                     ALPPLTELESLLFRSITVGFVLLTATLLTGLLFVDNLLAQHLVHKTVLSVLSWLAFGA
                     LLLGRWRRGWRGAIAVRWTLAAMALLVLAFFGSKFVLELVLHRTG"
     gene            5659..7026
                     /gene="ffh"
                     /locus_tag="PK399_07550"
     CDS             5659..7026
                     /gene="ffh"
                     /locus_tag="PK399_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPW13010.1"
                     /translation="MFESLTQRLSGTMERLRGRGRLSESNISEAVREVRIALLEADVA
                     LPVVQGLIQRIKVRAVGQEVMRSLTPGQVLVRIVRDELALVMGSQATDLNLNVPAPAV
                     ILMAGLQGAGKTTTVGKLAKHLKERRKKKVMVVSADVYRPAAIEQLQTLAGQVGALFF
                     PSDASQKPEAIVKAAIAEARKTFADVLIVDTAGRTSIDDAMMAEIKALHAAVNPVETL
                     FVVDSMTGQDAAVTAKHFGEALPLTGVVLTKTDGDARGGAALSVRYITGKPIKFVGTG
                     EKPDGLDVFHPDRAAGRILDMGDVLSLVEQVEQQVDHDKAKKLAEKVAKGKKFDLNDM
                     RDQLEQMQNMGGLHGLMDKLPGVGQLPEAVKQQVTGKEVPRMIAIINSMTKKERRNPD
                     LLNGSRRARVARGSGLTPADVNKVLKQYQQMEKMMGKLGRGGMKGMMRGLSGMMGGRG
                     GMPMR"
     gene            7122..7370
                     /gene="rpsP"
                     /locus_tag="PK399_07555"
     CDS             7122..7370
                     /gene="rpsP"
                     /locus_tag="PK399_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPW13011.1"
                     /translation="MVKIRLTRGGAKKRPFYHVIVTDSRSARDGRNIERVGFYNPVAQ
                     GAEKRVELNLERIKHWVDNGAQLTDKVADLYKAAAKAA"
     gene            7408..7929
                     /gene="rimM"
                     /locus_tag="PK399_07560"
     CDS             7408..7929
                     /gene="rimM"
                     /locus_tag="PK399_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407909.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPW13012.1"
                     /translation="MNAPDASQRMITVGRLHGAFGVRGEVKLESFTDPLHAIARYQPW
                     ILRDARGQERACEGVKLREGVKGLIASMPGVADRDAADALRGVEVRVPRSALPPPAAG
                     EYYWVDLEGLRVVNVEGVDFGTVSHLFSTGANDVLVAHGERERMIPFVQPDYIRSVDF
                     EAGLVTVDWDADF"
     gene            7941..8714
                     /gene="trmD"
                     /locus_tag="PK399_07565"
     CDS             7941..8714
                     /gene="trmD"
                     /locus_tag="PK399_07565"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPW13013.1"
                     /translation="MRFDIVSLFPEFVAQLAAHGVVGRAGERGLLSIHGWNPRDHAEG
                     NYRRVDDRPFGGGPGMVMMIDPLRAAIRAARDADPAPAKVIYLSPQGQRLTQAKVREL
                     AAMPRLVLLCGRYEGVDERLLQAAVDEEVSIGDYVLSGGELAAAVLVDAVARLQEGAL
                     NDAESAVQDSFEGDGLLDCPHYTRPVEHALGVVPDVLLSGNHAGIARWRRQQSLGRTW
                     LRRPDLLHEASLSKQDRSLLGEFRERWLSAVGEPPAGDA"
     gene            8873..9301
                     /gene="rplS"
                     /locus_tag="PK399_07570"
     CDS             8873..9301
                     /gene="rplS"
                     /locus_tag="PK399_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPW13014.1"
                     /translation="MNKPSIHTLVQDFETAQVSRKFPDFGPGDTVIVNVKVKEGNRER
                     VQAYEGVVIGKKNAGLNSAFTVRKISHGFGVERVFQTHSAAIDSVTVKRRGDVRAAKL
                     YYLRGLEGKKARIKEDLAGNAKAKAAAQAAAAAEAAAAAE"
     gene            9374..9781
                     /locus_tag="PK399_07575"
     CDS             9374..9781
                     /locus_tag="PK399_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPW13015.1"
                     /translation="MTMQIETVPGVRLDLWLWAARFYKTRALAKHAIETGKVDVGGQR
                     AKPSRSVRVGDALLVVRGEERFEVEVLASSTVRGPARVAQALYRESDASKLAREQARA
                     GRAAERAGFSPPESKPDKRARRLIRALGDIDAL"
     gene            9908..>10386
                     /locus_tag="PK399_07580"
     CDS             9908..>10386
                     /locus_tag="PK399_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015463366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPW13016.1"
                     /translation="MAATALTMGSRGEAVRRLQEALNRKLPGCALRVDGDFGNNTRVA
                     VRRYQDEAWLVVDGIAGQCTQNALFDAESYAPIRHQRPFIPQPTDNTCWAASTAMLKR
                     STVPAVIAATPADLIAGGGLSNSSNRADWEPATQRFAQAHGLRYFAPQSWTPQGFVNL
                     "
     assembly_gap    10387..10486
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10487..10803
                     /locus_tag="PK399_07585"
     CDS             <10487..10803
                     /locus_tag="PK399_07585"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HPW13017.1"
                     /translation="GLRYFAPQSWTPQGFVNLVRNGPVMVSTLWNVGGYLKRVPGGYE
                     GSPGHMTLVVGARGDGDPTGRGTSLMIYDPWEPNVGRRWRVSYYSWVTEVITRTYRLF
                     QQ"
     gene            complement(10859..13096)
                     /gene="katG"
                     /locus_tag="PK399_07590"
     CDS             complement(10859..13096)
                     /gene="katG"
                     /locus_tag="PK399_07590"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HPW13018.1"
                     /translation="MSSEAKCPFHQTAGGGTGNKDWWPNQLRLDLLNQHSDRSNPLGS
                     AFDYRKAFKGLDYAALKADLAALMTDSQDWWPADFGHYGGLFVRMAWHSAGTYRIGDG
                     RGGGGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQAISWADLMILAGNVALETM
                     GFKTFGFAGGREDTWEPDMDVYWGRETKWLGGDERYSRGSPGVDDPHGVLVKDDDSKA
                     PHTRDLENPLAAVQMGLIYVNPEGPDGKPDPIAAARDIRDTFARMAMDDEETVALIAG
                     GHSMGKAHGAASAENVGHEPEAADLELQGFGWANAHGSGKGADAITSGLEVTWTKTPT
                     RWSNNFFENLFGFEWELTRSPAGAQQWVAKDAEAIIPDAFDPSKKHRPTMLTTDLSLR
                     FDPAYEKISRRFLEDFDAFADAYARAWFKLTHRDMGPKSRYLGPEVPAEDLIWQDPIP
                     AAGHAAIDASDIATLKQAILGSGLSVGELVSAAWASASTFRGGDKRGGANGARVRLAP
                     QKDWAANDPAQLDKVLQALEGIKSDFDASAAGGKRVSLADLIVLGGCAAIEQAARDAG
                     ASVSVPFHPGRTDATQEQTDVDSFAPMEPFADGFRNYLKGPSEVPAEHLLVDKAQLLT
                     LTAPEMTALVGGLRVLGANSGGTAHGVLTDRPGTLSNDFFVNLLDMGTEWKPTGRNSY
                     AGHCRKTGAAKWTGTRVDLVFGSNSVLRALAEVYASADGAGKFVQDFVAAWSKVMHLD
                     RFDLA"
BASE COUNT         2147 a         4409 c         4666 g         2093 t
ORIGIN      
        1 cgctggaggt cgccgccgcc cagttgcatg acggactgca gcgtcgctgg atcctgcgtc
       61 gaaatctttt cggtcaaatg tgcgtgatgc acggcgccgc gctcggacca ggcatacgtg
      121 gtcagtgacg cggcgaagct gccgcccaat gtgcgcacga aggtggccag gccggagccc
      181 gcggcgatct cgtgcggttc caggtccgac agcaggatct gcagcaccgg catgaagaac
      241 agcgccacgc ccacgccctg gaacagctgg atccaggcca cgtgctcgaa gttgacgtcg
      301 aggttgaagt gcgagcgcgc gaagctggtg aacgacatcg cgatgaacgc gatactggcc
      361 agcatgcgca ggtcgaaccg gttcgcgtac ttgcccacga agggcgtcag cagcaccggc
      421 aggatgccga tcggcgcggt ggcaaacccg gcccagatcg cggtgtagcc caggttgcgc
      481 tgcagccaca gcggcaccag gatgccgaca ctgaagaacg ccgaatacgc caccaccatc
      541 gccagcgtgc cggcggcgaa gttgcgatgc cggaacaggc gcaggttgac gatcggatcc
      601 ttgtcggtga gttcccagat cacgaacacc gccagcgaga tcgcggcgat gatcgccagg
      661 accacgatgg tctgcgaggc gaaccagtcc tcgtcgttgc ccaggtccag caggatctgc
      721 agcgcgccca cgcccaggac cagcgtggcc agcccgatgt agtccatctt cggcgtttcc
      781 agccgctccg gccggccttt cagctggcga cccacgacca ggctggcgaa gatgccgatc
      841 ggcacgttga cgaagaagat ccattcccag ctgtagttgt cggtgatcca gccgccgaga
      901 atcgggccgg cgatcggcgc gaccacggtg accatcgcca gcaatgcgat cgcctgcccg
      961 cgtttcgccg gtgggtagat cgacagcagc agcgcctggg tgatcgggta catcggcccg
     1021 gcgacgaagc cctgcagcgc gcgcgcgacc accagcatgc ccatgctgtt ggcgatgccg
     1081 cacaggaacg aggcgatcac gaacgccatc gtcgaccaca cgaatagctt gcgctcgccg
     1141 aagcgccgcg ccagccagcc ggtcagcggc aacgcgatcg cggtgctgac cgcgaacgag
     1201 gtgatcaccc acgtcgcctg gttcgcgctg ccgcccaggt tgccggagat ggtcggcaac
     1261 gagacgttgg cgatggtggt gtccagcacc tgcatgaagc tggccagtgc caggcccagc
     1321 gtggtcagcg ccacgttcgg tgggcggaac gcggccgggc tggccgcagc cgggggcccg
     1381 gcaggtgcgt cttgcgcgat cgtggacatg cggcttcggc ttgttggaaa ggtgcaggag
     1441 cggcagcgcg ttggctgccg gctgggcgtc gcccgtcacc acgaggggag ggggagggaa
     1501 cgaagcgccg ccagcgcgac tcctgcgtaa gggaaggtca ggaccggcgg ccctgcgcca
     1561 ggttctggct gatgatcgtg tcgatgcggg cgtcggcgtc ctgcaactgc ttcgcgtacg
     1621 cctgcgtcgt cagcacgggt tttgccggcg cggccacggg caaggtcttg ccgccctggt
     1681 cgcggatgcc gacatcggcg gacatgctca tgcccaggcg cagcggatgc tcggcgacct
     1741 gcttcggatc cagttcgatg cgcaccggca cgcgctgcac gatcttgatc cagttgccgc
     1801 tggcgttctg cgcgggcagc aatgcgaacg cgctgccggt gcccaggccc aggctggtga
     1861 tcttgccggt gtacgtcacg tcgtcgccgt agatgtcggc ggtgagttcc accggttggc
     1921 ccaggcgcat cgaatgcaat tgggtttcct tgaagttggc ttccacccag acctgttcca
     1981 gcggcaccac ggtcatcagc gcggcgcccg gctgcacgcg ctggccgagt tgcaccgcac
     2041 gcttgccgac gaagcccgag atcggcgcga cgatcgccgc gcgctgcagg ttgaggtagg
     2101 cctggcgcag ttgcgcggcg gccgcctgca cctgcggctg gttgccgacg gtggtggcat
     2161 cgaccagcgc gcggttgcgc gacaactggc cctgcgagcc ggacagcgcg gcttcggcgg
     2221 catccagttg cgcccttgcg tgcgcgagct cctccgacga gaccgcgccg gtcgcgacca
     2281 ggccgctgcg gcgggccacg tcggcacgtg cctgcgccac cgcgacttgc cgcgcggcga
     2341 tgtcggcctg cgccgcgtca acgctgctgt agaggccgcg cacctggcgc acggtgttgg
     2401 cgaggttcgc ttcggcttgt gcgtacgcga cctgggcatc attcgggtcg agttgcacca
     2461 gcacctggcc ggcttcgacg cgcatgccgt cttcggcatt gatcgcgacc accgtgcccg
     2521 gcgtttgcgg ggtgatgctg acgatgttgc cctgcacgta ggcgtcgtcg gtcgactggt
     2581 gccaacgcgc gaccgtcagg taccaggcaa gccaggcgcc gccggcgatc agcacgaccc
     2641 cgagcaggat cagcagcgcg cgcttgcgct tgccattgcc ggcccgtgcg ggaggcgagg
     2701 gcggcagcgg cgcggcgttg ccggtggagg tgttcggcgt ggtttcggtc gtcatggcga
     2761 tatcagttcg aatgcgaagc agtggggttc ggcgagtcca cggcgatgcc gccgccgagc
     2821 gcctggtcga gatcgatgct ggcgatgcgg cgcgcggcga ccaatgcggc gagttgctgg
     2881 tccagtcgca gcagcggtgc ctgcgcggcg agtacgtcga gttgcgtggc cagcccggcg
     2941 cgctgccgtt gcagcacgag ggcatgtgcc ctggcggcgg catcgcgggc gacggtggtg
     3001 ctggcgacct gcgcgtccag cgcgcgtgcg gcctgcacgg cgttggcgac gtcgcggatc
     3061 gcgccgacca gcgtttcgtt gtagttggca acggccaggt cgcggtctgc atcgctggaa
     3121 cgcagttgct gttccaggcg cccgccttcg aagatcggca ggctcaaggc cgggccgcca
     3181 ttgagcagca gggcatcgct gccgaacagg tcgcccaggt tgccggcggc caggccggcc
     3241 agcgcgctga ggttgatcga gggatagaac gcggccttgc tggcgtcgat gccgtgttgc
     3301 gcggcttcga cccgccagcg cgcggcgacg atgtcggcgc ggcgtgccag caggtcgctt
     3361 ggcagcacgg acggcaacgc gatgccgggg gtcttgagca tcggcctggc gatctcgagg
     3421 ccacgatccg ggcccgcgcc gagcagcgcc gccagggcgt tgcgcatggc atcgatgtcg
     3481 tgctgcgcgg cctgtgcctg ctggcgcgcc gcagcggccg cgctgcggtt ctggttgagc
     3541 gcgatggtgt tgtccaggcc ggccttgatc cgctgctggt tgagcgaaat gaggccatcg
     3601 gcgcgcgcgg cttcggcgtt cgccgcgtcg agcgcgtcga aggcgcgggc caggcccacg
     3661 tagctgcgcg ccacgttcgc cgccagcgcc aggcgcgcgg attgcgcgtc cacttcggtc
     3721 gcgcgcgcgc tgcccacggc ggcctgccac ttgctgcgcg acacgcccca gacgtcgggg
     3781 ttccaggcga agctgagggt caacacattg acgaccttga agtcgccgcc gatctcgtcg
     3841 ccgaccagtg cgggtggcag ctgtgcgccc agcacctgcg cgccggcgcc aaccgtcggc
     3901 ttgcgcgcgg catcggccag gcccgcctgg gcgatggcct tgcgggtgcg cgcgtcggcg
     3961 gcttcgatcg aaggtgcgtt ggccagcgct tcgtcgacca gtgcatcgag ttgcgggtcg
     4021 ccgaatgcgg tccaccagcg ttcgctcggc cacgcggcat cggagacgcg ggtgccggcc
     4081 agcgatgcgg aggattgcag gctgccggca tcgcgcatcg cgccgcgcgg cgcgatgtcg
     4141 ccgctgctgg cgcaggcggt ggcgaggagg gcggtggcca gcgcggtggc caggagcgag
     4201 ggacgggaca tggttcagtc cgtgggggag ttcaggttgt cgcgcacgcg ttccagcagg
     4261 gcgagcagtt gcgcgcgttc tacgggttcc aggccctgca gggcggcttc gcgcacgcgc
     4321 tggccgcact ggtcgatgtc gcgccagatc gccttgccgg cgtcggtcag gtggatgtgc
     4381 agggcgcggc gatcgttcgg atccgccacg cgttccagga tcccgcgcgc ttccagcttg
     4441 tcgagcaggc gggtcatcgc gcccgggtgc aggtaggccg cgcgggcgag ctcggtgacg
     4501 ctggtggtgc tgttggccag cgtcttgatg gtgatgaact ggctgaaatt cagctcatgg
     4561 ccgcaacgcg ccagctccac ttccatccgc gcccacatcg cgtctcgggc ctgccggaac
     4621 agcaggccca gcgtggagcc gctgcagctg ccgatggcgg gtgccaccgg gtggtcaggg
     4681 gaatgcgggt tcgtcatggg acggcatgat ctgcgcccat gcaatagttg tcaatgcaat
     4741 tatctacaat gcaaacgtat tctcgccgca accgcgtgat caccccgtcc ggtgcaacac
     4801 cagctccagg acgaacttgc tgccgaagaa cgccagcacc agcagcgcca tcgccgccag
     4861 ggtccagcgc acggcgatgg cgccgcgcca gccgcgtcgc cagcgaccca gcagcagggc
     4921 gccgaaggcg agccaggaca gcacgctgag tacggtcttg tggaccaggt gctgggccag
     4981 caggttgtcg acgaagagca ggccggtcag cagggtggcg gtgagcagga cgaagccgac
     5041 ggtgatgctg cggaacagca ggctttccag ttcggtcagc ggcggcaggg cgcgcagcca
     5101 gccgcggaac tcacgccggc gcaatgcgcg ttcctgcgcc cacaggaaga tcgccagcac
     5161 ggccgacaac gccagcgtgg cataggccag cagggccatc catgcatgca gttgcaggcg
     5221 ccagccgagg ccgctggcat tgtccgcatg gccgtaggcg cgataggcca acaggctcgc
     5281 ggcggccagc gggaacacca cgatgccgag tgctggcatc cgcccggacg caccgaacgc
     5341 ggtggtcagc gccgccatgc ccagcccgac cagcgacagt gccgcaaaga agtgcatgtc
     5401 cgcgccttgc agcaggcgcc aggcctccgc atgggtttcg gcatggaaca gcacggcgag
     5461 caccgccacc ggaatccagc cgcgccgggg gccggcatcg ccggtggcgg agcgccagag
     5521 cagggcggct gcggccacgt agatcactac ggcgatgaga acgattgtca tcgcgcgagt
     5581 gtcgcacaaa ggtgcgacgg ccgggcgcgg gctatactcc gcggcccgaa atcccggcat
     5641 ctgcaaggct gcagccccat gttcgaatcc ctgacccagc gcctgtccgg caccatggag
     5701 cgcctgcgcg gccgtggccg gctgtccgaa tccaacatca gcgaagccgt gcgcgaagtg
     5761 cgcatcgccc tgctggaagc ggacgtggca ctgccggtgg tgcaggggtt gatccagcgg
     5821 atcaaggtgc gcgcggtcgg ccaggaagtg atgcgttcgc tcacgccggg ccaggtgctg
     5881 gtgcggatcg tccgtgacga gctcgcattg gtgatgggct cgcaggcgac cgacctcaac
     5941 ctcaacgtgc cggcaccagc ggtgatcctg atggccggcc tgcagggcgc cggcaagacc
     6001 accacggtcg gcaagctggc caagcacctc aaggagcgtc gcaagaagaa ggtgatggtg
     6061 gtcagcgccg acgtctaccg tccggccgcg atcgagcagt tgcagacgct ggccggccag
     6121 gtcggcgcgc tgttcttccc gtccgatgcg tcgcagaagc cggaggccat cgtcaaggcc
     6181 gcgatcgccg aggcgcgcaa gactttcgcc gacgtgctca tcgtcgatac cgccggccgc
     6241 accagcatcg acgacgcgat gatggccgag atcaaggcgc tgcacgccgc tgtcaacccg
     6301 gtcgaaaccc tgttcgtggt cgactcgatg acgggccagg acgccgcggt caccgccaag
     6361 cacttcggcg aggcgctgcc gctgaccggc gtcgtgctga ccaagaccga tggcgatgcg
     6421 cgtggcggcg cggcgctgag cgtgcgctac atcaccggca agccgatcaa gttcgtcggc
     6481 acgggcgaga agcccgatgg cctggacgtg ttccacccgg atcgcgccgc cggccgcatc
     6541 ctcgacatgg gcgacgtgct gtcgctggtc gagcaggtcg agcagcaggt cgaccacgac
     6601 aaggccaaga agctggcgga gaaggtcgcc aagggcaaga agttcgacct caacgacatg
     6661 cgcgaccagc tcgagcagat gcagaacatg ggcggcctgc acggcctgat ggacaagctg
     6721 cccggcgtcg gccagctgcc ggaggcggtg aagcagcagg tcaccggcaa ggaagtgccg
     6781 cggatgatcg cgatcatcaa ttcgatgacc aagaaggaac gccgcaaccc cgacctgctc
     6841 aacggttcgc gccgtgcccg cgtggcgcgt ggctccggcc tgaccccggc cgacgtgaac
     6901 aaggtgctga agcagtacca gcagatggaa aagatgatgg gcaagctggg ccgtggcggc
     6961 atgaagggca tgatgcgcgg cctgagcggg atgatgggcg ggcgcggcgg catgccgatg
     7021 aggtaatcga cgtccgaccg ggtttcgcca gccgattcaa cacgttatac ttgccggctt
     7081 acccggccat tccggccgct gggcaccacc gagaaactgc aatggtcaag atccgcctta
     7141 cccgtggcgg cgccaagaag cgccccttct accacgtcat cgtcaccgac agccgcagcg
     7201 cccgtgacgg ccgcaacatc gagcgcgtgg gcttctacaa cccggtcgcc cagggtgccg
     7261 agaagcgcgt cgagctgaac ctcgagcgca tcaagcactg ggtcgacaac ggcgcgcagc
     7321 tgaccgacaa ggtcgccgac ctctacaagg ccgccgccaa ggctgcctga tccggcgggc
     7381 cgcctcgcgg cccccgatcg cattcccatg aacgcaccgg acgccagcca gcggatgatc
     7441 accgtcggcc ggttgcatgg tgcgttcggc gtgcgtggcg aagtgaaact ggaaagcttc
     7501 accgatccac tgcacgccat cgcccgctac cagccgtgga tcctgcgcga cgcgcgcggg
     7561 caggaacgcg cctgcgaggg cgtgaagctg cgtgagggcg tcaagggcct gatcgcgagc
     7621 atgcccggcg tggcggatcg cgatgccgcc gatgccctgc gcggggtcga ggtgcgggtg
     7681 ccgcgctcgg cattgccacc gccggccgcc ggcgagtact actgggtcga cctggaaggc
     7741 ctgcgcgtgg tcaacgtgga gggcgtggac ttcggcaccg tctcgcacct gttctcgacc
     7801 ggcgccaacg acgtgctggt cgcgcatggc gagcgcgagc ggatgatccc gttcgtgcaa
     7861 ccggactaca tccgcagcgt ggatttcgag gccggcctgg tcaccgtcga ctgggacgcg
     7921 gacttctgac gtcgatgccg atgcgcttcg acatcgtcag cctgttcccc gaattcgtcg
     7981 cccaactcgc cgcgcatggc gtggtcgggc gcgccggcga gcgcgggctg ctgtcgatcc
     8041 atggctggaa cccgcgcgac catgccgagg gcaattaccg tcgggtcgat gaccgtccgt
     8101 tcggcggtgg tccgggcatg gtgatgatga tcgacccgct gcgcgctgcg attcgcgccg
     8161 cgcgcgatgc cgaccccgcg ccggccaagg tcatctacct gagcccgcaa ggccagcgcc
     8221 tgacccaggc caaggtgcga gaactggccg ccatgccgcg cctggtcctg ctctgtggcc
     8281 gctatgaggg cgtggacgag cgcctcctgc aggccgcggt ggacgaggag gtctcgatcg
     8341 gcgactacgt gctgtccggg ggcgagctgg ccgcagcggt actggtcgat gcggtggcgc
     8401 gcttgcagga gggcgcgttg aacgatgcgg aatcggcggt gcaggacagt ttcgagggcg
     8461 acggcttgct ggattgcccg cactacaccc gcccggtcga acatgcgctg ggcgtggtgc
     8521 ccgacgtcct gctgtcgggt aaccacgccg ggatcgcacg ctggcgccgg cagcaatcgc
     8581 tcggccgaac ctggctgcgc cggcctgatc tgctgcacga ggcgagcctg tcgaagcagg
     8641 accggagcct gctgggggag ttccgcgagc gatggctctc ggcggtgggc gagcctcccg
     8701 cgggagacgc ctaagcctct ggcgcggctg gaaaaatccg ggctataatg cgcggctggc
     8761 cgtaccgcac cacgttccac cgatgccatg acgcgggtcc acgtgcactc gcgcaacaac
     8821 tactccaaca ggcgcgtgca ccgcacgcca cgattccatc aaggttgatc ccatgaacaa
     8881 gccctcgatc cacaccctcg tccaggactt cgaaaccgcc caggtttcgc gcaagtttcc
     8941 cgacttcggc cccggcgaca ccgtcatcgt caacgtcaag gtcaaggaag gcaaccgcga
     9001 gcgcgtgcag gcctacgaag gcgtggtcat cggcaagaag aacgccggcc tgaactccgc
     9061 gttcaccgtg cgcaagatct cccacggctt cggcgtggag cgcgtgttcc agacccacag
     9121 cgccgcgatc gactcggtga cggtcaagcg ccgcggcgac gtccgcgccg ccaagctgta
     9181 ctacctgcgc gggctggaag gcaagaaggc gcgcatcaag gaagacctgg ccggcaacgc
     9241 caaggccaag gccgctgcgc aggccgccgc tgccgccgaa gcggccgccg ccgccgagta
     9301 atccgggcgc aacgtttcct gcaacggcca cccctgggtg gccgtttgca tttcaggagc
     9361 ctgatccccc gcgatgacga tgcagatcga aaccgtaccg ggcgtccgcc tcgacctgtg
     9421 gttgtgggcc gcgcggttct acaagacgcg cgcgctggcc aagcacgcca tcgagaccgg
     9481 caaggtcgat gtcggcggac agcgcgccaa gccatcgcgc agcgtgcgcg tcggcgatgc
     9541 gctgcttgtg gtgcgcggcg aggaacgctt cgaggtcgaa gtgctcgcgt cgagcaccgt
     9601 gcgtggcccg gcgcgcgttg cgcaggcgct gtatcgcgaa tccgatgcct cgaagcttgc
     9661 gcgcgaacag gcacgcgcag ggcgcgccgc ggaacgcgcc ggtttcagtc cgccggagtc
     9721 gaagcccgac aagcgcgcac gccggttgat ccgcgcactg ggcgatatcg acgcgttgtg
     9781 agtccagggg ggaacgtccg cctaggcgga accggatact ctggatggag ggtgtctatc
     9841 ccttcctgtg gatggcgccg ggttgtgggg gtgttcacca ttccgctcat cgaacaggag
     9901 tggtgacatg gccgcaacgg cattgacgat gggatccagg ggcgaggcgg tgcgtcgcct
     9961 gcaggaagct ctgaaccgca agctgccggg ctgcgcattg cgcgtcgatg gcgatttcgg
    10021 caacaacact cgcgtcgcgg tacgccgcta tcaagatgaa gcatggctgg ttgtcgacgg
    10081 catcgctggc cagtgcacgc agaacgcgct gttcgatgca gagtcctatg cgccgatccg
    10141 gcaccagcgc cccttcattc cccagccgac cgacaacacc tgctgggctg ccagcacggc
    10201 gatgctgaaa cggagcacgg tacctgcggt catcgccgcc acgcctgccg acctcattgc
    10261 cggcggcggg ttgagcaatt cctccaaccg cgccgactgg gagccggcta cgcagcgttt
    10321 cgcgcaagcg cacggcctgc gctatttcgc gccccaatca tggacgccac aaggcttcgt
    10381 gaacctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgg cctgcgctat
    10501 ttcgcgcccc aatcatggac gccacaaggc ttcgtgaacc tggtgcgcaa cgggccggtg
    10561 atggtcagca cgttgtggaa cgtgggcggc tacctgaagc gtgtcccggg tggctatgaa
    10621 ggcagtcctg gccacatgac gcttgtcgtt ggcgcgcgcg gcgatggcga tcccaccgga
    10681 cgcggcacca gcctgatgat ctacgatccg tgggagccga acgtcggtcg acgttggcgg
    10741 gtcagctact acagttgggt gaccgaggtg atcactcgca cctatcgctt gttccagcag
    10801 tgacctaagg aaggacggcc gcttgcgcga ccgtcctggt gagctcggga cgtggcaatc
    10861 aggccaggtc gaagcggtcc aggtgcatca ccttgctcca agcggcgacg aagtcctgca
    10921 cgaacttgcc ggccccatcg gcgctggcat agacctcggc cagggcgcgc agcaccgagt
    10981 tggagccgaa caccaggtcg acgcgggtgc cggtccattt cgcggcgccg gtcttgcggc
    11041 agtggcccgc gtagctgttg cggccggtcg gcttccactc cgtgcccatg tcgagcaggt
    11101 tcacgaagaa gtcgttgctg agggtgccgg gcctgtcggt gagcacgcca tgcgcggtgc
    11161 cgccgctatt ggcgccgagc acgcgcaagc caccgacgag tgcggtcatc tccggcgcgg
    11221 tcagggtcag cagctgtgcc ttgtccacca gcaagtgctc ggcgggaact tcggaaggcc
    11281 ccttcaaata gttgcggaat ccatccgcga acggctccat cggcgcgaat gaatcgacgt
    11341 cggtctgttc ctgcgtggca tcggtacggc cggggtggaa gggcacgctg acggatgcgc
    11401 ctgcatcgcg cgccgcctgt tcgatcgccg cgcacccacc cagcacgatc aggtcggcca
    11461 gcgacacgcg cttgccgccg gcggcggacg catcgaagtc cgacttgatc ccttccagtg
    11521 cctgcagcac cttgtcgagc tgggccggat cgttcgcggc ccaatccttt tgcggcgcca
    11581 accgcacgcg tgcgccgttc gcgccgccac gcttgtcgcc accgcggaag gtggatgcag
    11641 acgcccaggc ggctgacacc agttccccga ccgacagccc gctgcccagg atcgcctgct
    11701 tgagcgtggc gatgtcactc gcatcgatgg ccgcgtggcc ggcggccggg atcgggtcct
    11761 gccagatcag gtcctcggcc ggcacttcgg gccccaggta acgcgacttc ggacccatgt
    11821 cgcggtgggt cagcttgaac caggcgcgcg cgtaggcatc ggcgaacgca tcgaagtcct
    11881 cgaggaagcg gcgcgagatc ttttcgtacg cagggtcgaa gcgcagcgac aggtcggtgg
    11941 tgagcatcgt cggccgatgc ttcttcgacg gatcgaacgc atcgggaatg atcgcctcgg
    12001 catccttcgc gacccactgc tgcgcgccgg ccggactcct ggtgagttcc cattcgaagc
    12061 cgaacaggtt ctcgaagaag ttgttgctcc agcgcgtcgg cgtcttcgtc caggtcactt
    12121 ccaggccgct ggtgatcgcg tcggcaccct tgccgctgcc gtgcgcattg gcccagccga
    12181 agccctgcag ttccaggtcg gccgcttccg gctcgtggcc gacgttctcc gcgctcgccg
    12241 cgccgtgggc cttgcccatg ctgtggccgc cggcgatcag ggccacggtt tcctcgtcgt
    12301 ccatcgccat gcgcgcgaag gtgtcgcgga tgtcgcgggc ggcggcaatc ggatcgggct
    12361 tgccgtccgg gccttcggga ttcacgtaga tcaggcccat ctgcaccgcg gccagcgggt
    12421 tctccaggtc gcgggtgtgc ggcgccttgc tgtcgtcgtc cttgaccagc acgccatgcg
    12481 ggtcgtccac gccgggcgag ccgcgcgaat agcgttcgtc gccgccgagc cacttggtct
    12541 cgcggcccca gtacacgtcc atgtcgggtt cccaggtgtc ctcgcggcca ccggcgaagc
    12601 cgaaggtctt gaagcccatg gtttccagcg cgacattgcc ggccaggatc atcaggtcgg
    12661 cccaggaaat cgcctggccg tacttctgct tgatcggcca caacagccgg cgcgccttgt
    12721 ccaggctgac gttgtccggc cagctgttga gcggggcgaa gcgctgctgc ccgcggccgc
    12781 cgccgccgcg gccatcaccg atgcgatacg tgccggcgct gtgccaggcc atgcgcacga
    12841 acaggccgcc gtagtggccg aaatcggccg gccaccagtc ctgcgaatcg gtcatcagcg
    12901 cggccaggtc ggctttcagc gccgcgtaat cgaggccctt gaaggccttg cggtagtcga
    12961 acgcgcttcc cagcggattg gagcggtcgg agtgctggtt gagcaggtcg agccgcagtt
    13021 ggttgggcca ccagtccttg ttgccggtac cgccgcctgc tgtctggtgg aacggacact
    13081 tcgcttcgct ggacatgatg gctcgcctcc cggctcgctg tgatcgaccg gacccatcct
    13141 acgcaggcat tcacgaaaga aaaattgaat gatgtgaagt gttcgatagg gacgccctat
    13201 ccgcagtgca gcaaaactgc gcggcccgtc accgcaagcc gcatgcagga cggctgggct
    13261 gcggccatcg ccggatatgc ggtgccgccc gcatcgccag gggtatggtc aggccagcga
    13321 cttcagccgg tacaacgcct cgagggcctg ctttggcgag agctcgtccg ggtcgatgcc
    13381 ggcgagcgca tccagcgccg ccgagcgcga cgcga
//
LOCUS       DAOOCX010000066        13224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_4327_length_13224_cov_7.191662, whole genome shotgun sequence.
ACCESSION   DAOOCX010000066 DAOOCX010000000
VERSION     DAOOCX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 13224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13224
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4327_length_13224_cov_7.191662"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PK399_07595"
     CDS             complement(<1..382)
                     /locus_tag="PK399_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPW13019.1"
                     /translation="MRPFPLGEDIDALREAVRRFADNEIAPRAAQIDEENAFPQDLWP
                     KLGEMGLLGITVPGEFGGSEMGYLAHLVAMEEISRASGSVGLSYGAHSNLCVNNLYAN
                     GNAAQRAKYLPKLCSGEWKGALAMS"
     gene            complement(529..1629)
                     /locus_tag="PK399_07600"
     CDS             complement(529..1629)
                     /locus_tag="PK399_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HPW13020.1"
                     /translation="MIFETLDTYGHEQVVFCHNKDAGLKAIIAIHNTVLGPALGGTRM
                     WPYKSEAEALNDVLRLSRGMTYKNAVAGLNIGGGKAVIIGDPATDKSEALFRAFGQFV
                     ESLGGRYITAEDVGIDVNDMEYVYRETEYVTGVHQVHGGSGDPSPFTAYGTLQGLMAT
                     LNKKYGDEDVGKYSYAVQGLGHVGMEYVKLLKERGAKIFVTDINKALVEKAVTEYGAE
                     AVGLDEIYDVPADVYSPCALGGTVNEQTLPRLKAKVICGAANNQLANNAIGDEVARRG
                     ILYAPDYAVNAGGVMNVALELDGYNRERAMRMMRTIYHNLTRIYEISDREGVPTYQAA
                     DRLAEERITTIGKLKLPLGRSQPRFQGRIRGH"
     gene            2108..2842
                     /locus_tag="PK399_07605"
     CDS             2108..2842
                     /locus_tag="PK399_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13021.1"
                     /translation="MPVPASEPAMPEPAPPAPLVMAPAPVAAKPVPPRKPVLPKPETK
                     PVPAAEPAKPVASGSTVTGRLELVAGGAGAVLPGELADGLVYFLPKAGAPKPKPGNFS
                     IDTRSKGFSPALLVVPQGSTVRFPNRDTILHNVFSRTPGSSFDFGHYGPGESKQYVFN
                     KPGLVIVNCNVHHNMRADVVVLATPYYTRPDRNGRYTLAGLPAGPGTLVFWHPRAQAQ
                     SVAVALPASAPVVRKLTATRPARQEH"
     gene            2846..3922
                     /locus_tag="PK399_07610"
     CDS             2846..3922
                     /locus_tag="PK399_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13022.1"
                     /translation="MAALHGTRLRILLALVIVLPALLALLALQFARDAAREASRTLST
                     LAMTQQLVEEQANRNLTIRGELIAGNQAVVAYMTQALGSLLPGEAVDYSSIVDLLEER
                     RSQLDLDLAAVVAADGRALAVTDAYAEGHDFANDPVFVEARKRQSVGTGLWRDGDRLL
                     HVAMLPLARYNAGDAYLLVAEEVDQDYVKTIAGIAAADVALVATSASGPGVLASTLPA
                     TDADALSKSIARWPAGDASSTGQLHLAAGSSQARLTALFGNQEVRVVQIARRMPLSAT
                     LAAHVPIVLLAMLSLVALLGAIAWYGRRVASPLLALERLLERAASTSDRNLHMDPAGS
                     AEVSRVATAFNQLMAKGQQADRHG"
     gene            3963..4922
                     /locus_tag="PK399_07615"
     CDS             3963..4922
                     /locus_tag="PK399_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HPW13023.1"
                     /translation="MTPACNAVRPHRRRAAPFACATLLLLGSGMAQAQDAAALDAPAE
                     EPSLQDPPPANSGKLLLTGGVTQVEGAAGGGLAPWAVIGGQGTRDQVGATAYYTRVDV
                     GDYALDSYGALVGLHDRVELSVARQAFDTRAVGAALGLGQGYVIRQDIIGVKVKLAGD
                     AVLEQDRWMPQLAIGLQRKRNNRGALLSAIGARDDSGTDVYLSATKLYLDHSLLLNTT
                     LRFTKANQFGILGFGGDRNDGYKPQFEGSAAFLLNRHVAVGAEFRSKPDNLGIASEDN
                     AWDAFVAWAPNKHVSLTVAYVDLGNIVIRDHQRGWYASLQVGF"
     gene            4937..5437
                     /locus_tag="PK399_07620"
     CDS             4937..5437
                     /locus_tag="PK399_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HPW13024.1"
                     /translation="MRIRSIQLVIAGLLGTVQLAATAFAQSTAGAPQAATSRAPNPAP
                     VHPELAGVFEQFGGLAGLTALNEEFMALMLADPRLEPYFRDVDRARVTRQLTEQFCAV
                     LGGGCTYSGRDMVEAHAAFKIERAHFNALVEVLQVAMDKRGIPFRAQNKLLAVLAPMH
                     REVVND"
     gene            complement(5475..8483)
                     /locus_tag="PK399_07625"
     CDS             complement(5475..8483)
                     /locus_tag="PK399_07625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW13025.1"
                     /translation="MRRTLSVAFLLTCLAPASAGVPAPTAGANLHGLGWLPSDAYDTG
                     EGLPDPTVNAIAALPNGHVWLGTMRGLARLNGARVVAEPGPGGVLGKPILGLAGTADG
                     DLLASVDAAGVYRLHDGQWQSLGSPFGSNRTQRLRAFGKPGAVRVFATGNGVAEWNGA
                     RWEPRALPAPLAGREVFDIALQPASDGIAETLWVASFGGGLYRCIGKAACERVAIDGS
                     GPRTDEIRTLQLQPRAGKPDALWLALQGGGVARLADGGWTRWHAGNSTLPSDFVSDLA
                     LVEVPGAGLDVWAGTRSGLAILRDGREWIGADPRIPQLRERVRTLALTRTSQGVPVMW
                     AGSDTGAVRTPLAGPWRLVSRLGSSGNGIWDVWVEPATDGGERVWLASDGEGLARFED
                     GHWRRYGRADGMPSDSARSIRRVPDGSREGSLWIGTWGGHVARLQGERFVELPTPWKK
                     LPGDAASLLLADRDDVWVGTREQGMAHWDGRQWTWFPPGRDMPGWVYAAVRVGDTLWF
                     SSAGKGLVRRRGGEWRFFGKDIGLPADRFYDLRLIPDADGRPVLWMGSSSNGLVRVDV
                     GNPDKPRLVTDPALPALPIAKAYGAVRDGRGDIMVCTDYGVFSWRPAGERYLATAYHR
                     EDGVPHDECNGKALQVDERGRVWIGTVGGAAVYTPPEATPRRPSPLELTGLRVDGRQV
                     RVVDGVLALPRADSALELHYDLLTGEKEAGSRYRVSLLGREGGIGTWEPLDSHAFARL
                     PAGRQRVRIEARDFAGVPAEPLELVVDVPQVWWQTPLARALQVLGALALFWGVLKLRE
                     RQLRLRELQLRDMVRDRTNQLQASDTELRRANDELRRLSYTDPLTGLGNRRRLFEALE
                     LHCRHGHEQQRPLGLLLMDLDHFKAINDSHGHTAGDLCLQSVARVMLATLPGGAIAAR
                     YGGEEFCVVLPGLDLAAATAVAEDLRAAVQAIPPLDCQANGPRVTASIGAVSRVPNGP
                     ACADLLLTLADGALYRAKAAGRNRVQPATP"
     gene            8649..10634
                     /locus_tag="PK399_07630"
     CDS             8649..10634
                     /locus_tag="PK399_07630"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW13026.1"
                     /translation="MQIPFKPTALAIALACLVPAGLAFAAEPQAAPAAAETTVTTPGG
                     RATFLLPQGWASRVQGNAVIVTPPEADGSRSAFVDTTATDVDAAVAEAWKVLGLDPKF
                     LLATDAAPRDGWDQRRFYDYDIPANAKRVVNASAFRKGKAWTVVVADVDQAIAEKRAS
                     QYGKIAQRFQPAGYARETFAGRAAHKLDAAKLAQVADFIERMRQQFEVPGVAIGIVQD
                     GKVVMSRGFGVRELGKPAPVDGDTRFMIASNTKALTTLMLAKLVDAGKLDWNARAEDV
                     YPAFKLGDAATTDKVLVKHLICACTGLPRQDMEWLFEGEKQTPETVMSTLGTMQPTSG
                     FGELFQYSNPMAAAAGYIGGQVAYPGGELGASYDKAMQALVFDPLGMRNTTFDYAKAQ
                     TGNYAAPHGFDIDHKVEVMDMGLNDTIVASRPAGAAWGTVNDLLRYVQMEIDRGVLPD
                     GTRYIGEAALLQRREPQIALGVDRNYAMALMVDKSDGVTVVDHGGDMGGFHSNMMWWP
                     EQKVGAVILTNADEGVYLRGPFKRRLMELMFDGEAQAQATADANAKANREGFDAFRKL
                     LQIPGDAAALAALAPRYRNDALGDIVVSRRDGKARIDVGAFSSEIVTQPQPDGSMAFV
                     TIDPVAAGFQLVRADKDGMRKLVVRDGQHEYVFDEVK"
     gene            complement(10671..11477)
                     /locus_tag="PK399_07635"
     CDS             complement(10671..11477)
                     /locus_tag="PK399_07635"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HPW13027.1"
                     /translation="MPIRPLSCALLACLLSACMHTPPASAPRVLLLGEVHDSAAGHAK
                     RAAWLADEVAAGWRPVIAMEQFDTDQQAALDAAMRDCADADCVVAKVVPAKTGWTWEY
                     YKPLLTLALQYGLPVRAANLSRSEASKVIKGGFTAALSPDLVARYQLDALPAPLLAAQ
                     EAEVRDSHCGALPEAMVSPMAKAQIARDVVMADTMRTHAATGVVLIAGNGHVRKDIAV
                     PFWLRRDGLAPHAVGFLEPTSDAAVFDETRRIPATDRPDPCAGFKAPKAG"
     gene            11562..>13224
                     /locus_tag="PK399_07640"
     CDS             11562..>13224
                     /locus_tag="PK399_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806050.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPW13028.1"
                     /translation="MSRTAETLSQPQSESTPLRFVTAASLFDGHDAAINIMRRLIQAQ
                     GAEVVHLGHNRSVEDVVRAALQEDADGIALSSYQGGHVEYLKYMVDMLRERDAGHVKV
                     FAGGGGTITPEEIRELEAYGVERIYHPNDGMKMGLVEMIEDVVTRAGEGRENRGNRGQ
                     SRLSSKSDKARQHLEGNVDSDPGFPSLSDEIAIGRVLSAIEDGDYAEAELSRLRKEWQ
                     LAGGKTPVIGITGTGGAGKSSVTDELLNRFLASFPKMHIAVISVDPTRRRTGGALLGD
                     RIRMNSLRSHRVYMRSMATRRQNVATNAVLKDCIGFLKSLGYDLVIVETAGIGQSDSE
                     IVDLVDFPMYVMTSDYGAASQLEKIDMIDFAELIVLNKYDKRGAEDALRDIRKQWKRN
                     RVAFQTADADVPVYPTIASQFNDPGISWMFVNLCRLLREKWGQSRLSNIGNSGGEAGA
                     GKVDSDPTFLRPNLDTSLKEPRATVLIPGARVRYLAEIAEQGRAINRRIETQAEIADR
                     AQSLWQALQEIGDAALPKQLDLYPDDALTSEGDRSLLTLRQRYNDA"
BASE COUNT         2058 a         4664 c         4521 g         1981 t
ORIGIN      
        1 cgctcatcgc cagcgcgccc ttccattcgc cgctgcacag cttcggcagg tacttcgcgc
       61 gctgtgcggc gttgccattg gcgtagaggt tgttgacgca gagattcgaa tgcgcgccgt
      121 agctcaggcc gacggatccc gacgcgcggc tgatttcctc catggcgacg aggtgggcga
      181 ggtagcccat ctcgctgccg ccgaactcgc ccggcacggt gatgcccaga agccccatct
      241 cgccgagttt cggccagagg tcctgcggga acgcgttctc ttcatcgatc tgcgcggcgc
      301 gcggcgcgat ctcgttgtcg gcgaagcggc ggaccgcctc gcgcagtgca tcgatgtctt
      361 cgccgagggg aaatggacgc atcacgaacc tcttttctgg tgggttccca cagccccctt
      421 tagtaaaggg ggcgccgcga cagcggcggg gatttggtag cgacctgtgg ggcccaaagt
      481 ttgcacgcaa actttgggat tgatctgggc gcagtccaga tcaatacgtc agtgcccgcg
      541 gatcctgccc tggaatcgcg gctgcgaccg gcccagcggc agcttgagct tgccgatggt
      601 ggtgatgcgt tcctcggcca ggcgatcggc ggcctggtag gtcggcacgc cttcgcggtc
      661 gctgatttcg tagatgcggg tgaggttgtg gtagatcgtg cgcatcatcc gcatggcgcg
      721 ctcgcggttg tagccatcga gttccagcgc cacgttcatc acgccgcccg cgttcaccgc
      781 gtagtccggc gcgtacagga tgccgcggcg cgcgacttcg tcgccgatcg cgttgttcgc
      841 cagctggttg ttggccgcgc cgcagatgac cttggccttg agccggggca gggtctgctc
      901 gttgacggtg ccgccgagcg cgcacggcga gtacacgtcg gccggcacgt cgtagatctc
      961 gtccaggccc accgcctcgg cgccgtactc ggtcacggcc ttctcgacca gcgccttgtt
     1021 gatgtcggtg acgaagatct tggcgccgcg ttccttcagc agcttcacgt actccatgcc
     1081 gacgtggccc aggccctgca cggcgtagga atacttgccc acgtcctcgt cgccgtactt
     1141 cttgttgagc gtcgccatca ggccctgcag ggtgccgtag gcggtgaacg gcgaggggtc
     1201 gccggagccg ccatgcacct ggtgcacgcc ggtgacgtac tcggtctcgc ggtacacgta
     1261 ttccatgtcg ttgacatcga tgccgacgtc ctcggcggtg atgtagcggc cgcccagcga
     1321 ttccacgaac tggccgaacg cgcggaacag cgcctccgac ttgtcggtgg ccgggtcgcc
     1381 gatgatcacc gccttgccgc cgccgatgtt caggccggcg accgcgttct tgtaggtcat
     1441 gccgcggctc aggcgcagca cgtcgttgag ggcttccgcc tcgctcttgt acggccacat
     1501 gcgggtaccg cccagggccg ggcccagcac ggtgttgtgg atggcgatga tcgccttcag
     1561 gcctgcgtcc ttgttgtggc agaacacgac ctgttcgtgg ccgtaggtat cgagggtttc
     1621 gaagatcatt gcgggaactc cggccggagc cgctcgcatg tcgcgatggc gccgacggtc
     1681 tggaagggac gggaacggat gtgaagtgct cgcctgcacc ggatgcggca agggccggcg
     1741 gtgcagggcg cgcattctaa gccatcggtc ttggcggggc ttgaatacgg gcgggtacgg
     1801 ggccgcaacg ccgcgctggc gcctgccgcg gcggcatcgg agctgaatcg ccacccgctg
     1861 catccgcggc gcgtgcgcgc ccgtatgcac ctgtcgtggg gtcgaatgcc gggcaagcgg
     1921 ggcgcttgcg gttcgatcca gggggaacac gatgaaccga ttccgagccg tcgccggggc
     1981 ctttgccctg tgcggcatgg tcgcttgcgc caccgcgccc gcgcccgatc aaccgccgcc
     2041 gatggcggac gatatcgcgc ctgcacccgt cgagatggca cacgagcacg cgcctgagcc
     2101 cgtcccgatg ccagtgccgg catccgagcc ggcgatgccc gaacccgcgc cgccggcacc
     2161 actcgtgatg gcgccagcac cggtggccgc gaaacccgtg ccgccgcgga agccggtact
     2221 gccgaagccg gagaccaagc ccgtgcctgc cgccgagccc gccaagccgg tggccagcgg
     2281 cagcaccgtg accggccgcc tggaactggt cgcgggcggt gccggcgcgg tcctgccggg
     2341 cgagctggcc gatggcctgg tgtacttcct gccgaaggcc ggggctccca agccgaagcc
     2401 cggcaatttc agcatcgata cccgcagcaa gggcttctcg cccgcgctgt tggtggtgcc
     2461 gcaaggcagc acggtgcgct tccccaatcg cgacacgatc ctgcacaacg tgttctcgcg
     2521 cacaccgggc agcagcttcg acttcggcca ttacggcccg ggcgagagca agcaatacgt
     2581 gttcaacaag ccgggcctgg tgatcgtcaa ctgcaacgtc caccacaaca tgcgtgccga
     2641 cgtggtggtg ctggccacgc cgtattacac gcggcccgat cgcaatgggc gctacaccct
     2701 ggctggcctg cctgcgggcc cgggcacgct ggtgttctgg catccgcggg cgcaggcgca
     2761 atcggtggcg gttgccttgc cggccagcgc accggtggtt cgcaagctca ccgccacccg
     2821 ccctgcgcgg caggagcact gagccatggc cgcactccac gggacgcgcc tgcgcatcct
     2881 cctggcgctg gtgatcgtgc tgccggcgct cctcgccctg ctggccctgc agttcgcccg
     2941 cgacgcggcg cgcgaggcgt cgcgcacgct gtccaccctg gccatgaccc agcaactggt
     3001 cgaggagcag gccaaccgca acctaaccat ccgtggcgag ttgatcgccg gcaaccaggc
     3061 ggtggtggcg tacatgaccc aggcgctggg cagcctgctg ccgggcgaag cggtggacta
     3121 ctcctcgatc gtcgacttgc tggaggagcg caggtcgcaa ctcgacctcg acctggccgc
     3181 ggtggtcgcc gccgatggtc gcgcgctggc ggtgaccgat gcctatgcgg aaggccacga
     3241 cttcgccaac gatccggtgt tcgtcgaggc gcgcaagcgc cagtcggtgg gcaccgggct
     3301 gtggcgcgac ggcgaccgcc tgctgcacgt ggcgatgctg ccgctggccc gttacaacgc
     3361 cggcgatgcc tacctgctgg tcgcagagga ggtcgaccag gactacgtga agaccatcgc
     3421 cgggatcgcg gcggcggatg tcgccttggt cgcaacgtcc gcgtccggcc caggcgtcct
     3481 ggcctcgacg ttgccggcca ccgatgccga tgccctgtcg aagtcgatcg cgcggtggcc
     3541 ggcaggcgac gcctcgtcca caggccagct gcacctggcg gcggggtcgt cgcaggcgcg
     3601 cctcaccgca ttgttcggca accaggaggt gcgcgtggta cagatcgcgc ggcgcatgcc
     3661 gctgtccgcc accctggcgg cgcacgtccc catcgtcctg ctcgcaatgc tgtcgctggt
     3721 cgcgctgctg ggcgccatcg cctggtacgg ccgccgcgtg gcgagcccgc tgctggcgct
     3781 ggaacgcctg ctggagcgtg ccgcgagcac ctccgatcgc aacctgcaca tggacccggc
     3841 cggcagcgcc gaggtcagcc gcgtggccac ggccttcaac caactcatgg ccaagggcca
     3901 gcaggcggat cgccatggct gacctgccgt tcgccatccc ttccgttcgc ctggagagtc
     3961 cgatgacacc tgcctgcaat gccgtgcgac cgcaccggcg acgcgccgct ccattcgctt
     4021 gcgccacgtt gttgctgctc ggcagcggga tggcgcaggc gcaggatgcc gcggcgctgg
     4081 atgcgcccgc cgaggaacct tcgttgcagg atccgccacc ggccaacagc ggcaagctgc
     4141 tgttgaccgg cggcgtgacc caggtcgagg gcgcggccgg tggcggcctt gcgccatggg
     4201 cggtgatcgg aggccagggc acccgcgacc aggtcggcgc cactgcgtac tacacgcggg
     4261 tcgacgtcgg cgattacgcg ctggacagtt acggcgcgct ggtcggcctc cacgaccggg
     4321 tcgaactcag cgtggcgcgc caggcgttcg acacgcgcgc ggtgggcgcc gcgctgggcc
     4381 tggggcaggg ctacgtgatc cgccaggaca tcatcggcgt caaggtgaag ctggcgggcg
     4441 atgcggtgct ggaacaggac cgctggatgc cgcaactggc gatcggcctg cagcgcaagc
     4501 gcaacaaccg cggcgcgttg ttgtcggcga tcggcgccag ggacgacagc ggcaccgatg
     4561 tctatctcag cgcgaccaag ctctacctcg accacagcct gctgctgaac accacgctgc
     4621 gtttcaccaa ggccaaccag ttcggcatcc tcggtttcgg tggcgatcgc aacgacggct
     4681 acaagccgca gttcgaaggc tccgctgcgt tcctgctcaa ccgccacgtg gcggtcggcg
     4741 cggagttccg cagcaagccg gacaacctcg gcatcgccag cgaagacaac gcctgggacg
     4801 ccttcgtggc gtgggcgccg aacaagcacg tctcgctgac cgtggcctac gtggacctcg
     4861 gcaacatcgt catccgcgac caccagcgcg gctggtacgc ctcgctgcaa gtcggcttct
     4921 gaacatggag aacctcatgc gcatccgatc catccagctc gtgattgcag gcctgctggg
     4981 caccgtccag ctcgccgcaa cggccttcgc ccagtcgacg gctggagcac cgcaagccgc
     5041 gaccagtcgc gcaccgaatc ccgcacccgt ccaccccgag ctggccggtg tgttcgagca
     5101 gttcggtggc cttgccggac tgaccgcgct caacgaagag ttcatggcgc tgatgctggc
     5161 cgatccgcgg ctggaaccgt atttccgcga tgtcgaccgg gcgcgcgtga cccgccagct
     5221 gaccgagcag ttctgcgcgg tgctcggtgg cggctgcacc tacagcggac gcgacatggt
     5281 ggaggcgcac gcagcgttca agatcgagcg cgcgcatttc aacgcgctgg tggaagtgct
     5341 gcaggtggcg atggacaagc gcggcatccc gttccgtgcg cagaacaaat tgctggccgt
     5401 gctcgcaccg atgcatcgcg aagtggtcaa cgactgacgg gtcgcatccc ggcgaacgac
     5461 gcgccgtgtc gccatcacgg cgtggccggt tgcacccggt tgcgcccggc ggccttggcg
     5521 cggtacagcg cgccatcggc cagggtcagc aggaggtcgg cgcaagccgg cccgttcggt
     5581 acccgcgaga ccgcgccgat gctggcggtc accctgggac cgttcgcctg gcagtccagc
     5641 ggcgggatgg cctgcacggc ggcgcgcagg tcctcggcga cggccgttgc cgcggccagg
     5701 tccagcccgg gcagcaccac gcagaactcc tcgccgccgt agcgggcggc gatggcgcca
     5761 ccggggagcg tggccagcat gacccgcgcg acggactgca ggcagaggtc gcctgcggtg
     5821 tggccgtggc tgtcgttgat cgccttgaag tggtccaggt ccatcagcaa caggcccagc
     5881 ggtcgttgct gttcgtggcc atgccggcaa tgcagttcca gcgcttcgaa cagccggcgg
     5941 cggttgccca gcccggtcag cggatcggtg taggacagcc ggcgcagttc gtcgttggcg
     6001 cgacgcagtt cggtgtcgct ggcctgcaac tggttggtgc ggtcgcgcac catgtcgcgc
     6061 aactgcagtt cgcgcagtcg caactggcgt tcgcgcagct tgagcacgcc ccagaacaac
     6121 gcgagcgccc ccaggacctg cagcgcgcgc gccagtggcg tctgccacca cacctgcggc
     6181 acgtcgacca ccagttccag cggctcggcc ggcacgccgg cgaaatcgcg cgcctcgatg
     6241 cgcacgcgct ggcggcctgc gggcaggcgg gcgaaggcgt ggctgtccag cggctcccac
     6301 gtgccgatcc cgccttcccg gccgagcagg ctgacccgat agcgcgaacc cgcttccttc
     6361 tccccggtga gcaggtcgta gtgcaattcc agcgcgctgt cggcgcgcgg cagggcgagg
     6421 acgccgtcca ccacgcggac ctgcctgcca tccacgcgca ggccggtcag ctccaggggc
     6481 gacggccgcc gcggcgtcgc ctcgggcggg gtgtacaccg ccgcgccgcc gacggtcccg
     6541 atccacaccc gaccgcgttc gtcgacctgc agggccttgc cattgcactc gtcgtgcggc
     6601 acgccgtctt cgcggtggta ggcggtggcg aggtagcgct caccggcggg ccgccagctg
     6661 aacaccccgt agtccgtgca caccatgatg tcgccgcggc catcgcgcac cgcgccgtag
     6721 gccttggcga tcggcaaggc cggcaacgcg gggtcggtga cgaggcgcgg cttgtccgga
     6781 ttgcccacgt cgacgcgcac cagcccattg ctgctgctgc ccatccacag caccggcctg
     6841 ccgtcggcat cggggatcag gcggaggtcg tagaagcggt cggccggcag gccgatgtcc
     6901 ttgccgaaga accgccactc gcccccgcgg cggcgcacca ggcccttgcc ggcactgctg
     6961 aaccacaggg tgtcgcccac gcgtaccgcc gcatacaccc agcccggcat gtcgcgcccg
     7021 ggcgggaacc atgtccactg ccggccgtcc caatgcgcca tgccctgttc gcgggtgccg
     7081 acccagacgt cgtcccggtc ggccagcagc aggctggcgg catcgcccgg cagctttttc
     7141 cagggcgtgg gcaattcgac gaagcgctcg ccctgcaggc gtgcgacatg cccgccccag
     7201 gtgccgatcc acaggctgcc ctcgcggctg ccgtcgggca cccttcggat gctgcgggcg
     7261 ctgtcgctgg gcatgccatc ggcgcggcca tagcgccgcc aatgtccgtc ctcgaagcgg
     7321 gccagccctt cgccgtcgct ggccagccag acccgttcgc cgccatccgt ggccggttcc
     7381 acccagacat cccagatgcc gttgccgctg ctgcccaacc ggctgaccag ccgccacgga
     7441 ccggccagcg gggtgcgcac cgcgccggtg tccgagccag cccacatgac cggaacgccc
     7501 tggctggtgc gcgtcagggc cagcgtgcgc acgcgttcgc gcagttgcgg gatgcgcgga
     7561 tcggcaccga tccactcgcg gccgtcgcgc aggatcgcca gcccgctgcg ggtcccggcc
     7621 cacacgtcca ggcctgcgcc cggcacctcg accagggcga ggtcggacac gaaatcgctg
     7681 ggcagcgtgc tgttgccggc atgccagcgc gtccatccgc cgtcggccag gcgcgccacg
     7741 ccgccgccct ggagggccag ccacaacgca tccggcttgc ccgcgcgcgg ttgcagttgc
     7801 agggtgcgga tctcgtcggt gcgcggaccg ctgccatcga tcgccacgcg ctcgcacgcg
     7861 gccttgccga tgcagcgata cagcccgcca ccgaagctgg cgacccacag ggtctcggcg
     7921 atgccgtcgc tcgccggttg cagggcaatg tcgaagacct cgcgcccggc cagcggcgcg
     7981 ggcaacgcgc gcggctccca gcgcgcgccg ttccattccg cgacgccgtt gccggtggcg
     8041 aacacccgca ccgcacccgg tttcccgaat gcccgcaggc gctgggtgcg attgctgccg
     8101 aacggcgagc ccagcgactg ccactggccg tcatggaggc gatacacgcc ggccgcatcg
     8161 accgatgcca gcaggtcgcc gtcggcggtg ccggccagtc ccaggatcgg tttgccgagg
     8221 acgccgcccg ggccgggctc ggcgaccacg cgggcgccgt tgaggcgggc aaggccgcgc
     8281 atcgtcccaa gccagacatg gccattcggc aatgcggcga tcgcgttgac ggtcgggtcg
     8341 ggcaggcctt cgccggtgtc gtacgcgtcg gagggcaacc agcccaatcc atgcaggttc
     8401 gcccccgccg tcggcgccgg cacccccgcc gacgcaggcg ccaggcaggt gagcaggaac
     8461 gcgacggaca gggtgcggcg catgagcctc cggattctcc aagaatcagg aagcaaactc
     8521 catgccatcg cgtggatccg gcgacaggcg ggcaatccag cgggtcgagg gcttggccgt
     8581 gtcccgcccc ggatcgcgtg gacgcaagga cgggcacgcg cgcccgatgc ccgcttccgg
     8641 agacgacgat gcagatcccg ttcaagccca ccgccctggc catcgcgctt gcctgcctcg
     8701 tgccggccgg cctggcgttc gccgccgagc cgcaggccgc ccccgccgcc gcggagacca
     8761 ccgtcaccac ccccggcggt cgcgccacgt tcctgctgcc gcaaggctgg gcgtcgcggg
     8821 tgcagggcaa tgcggtgatc gtcaccccgc cggaggcgga cggctcgcgc agcgcgttcg
     8881 tggataccac cgccaccgac gtggacgccg ccgtcgccga ggcctggaag gtgctgggcc
     8941 tcgacccgaa attcctgctg gccaccgacg ccgcgccgcg cgacggctgg gaccagcgcc
     9001 gcttctacga ctacgacatc cccgccaacg ccaagcgcgt ggtcaacgcc agcgccttcc
     9061 gcaagggcaa ggcctggacg gtcgtggtgg cggacgtgga ccaggccatc gccgagaaac
     9121 gcgcgtccca gtacggcaag atcgcccagc gtttccagcc cgccggctat gcgcgcgaga
     9181 ccttcgccgg ccgcgccgcg cacaagctcg atgccgcgaa gttggcgcag gtcgccgatt
     9241 tcatcgaacg gatgcgccag cagttcgaag tgcccggcgt ggcgatcggc atcgtccagg
     9301 acggcaaggt ggtgatgtcg cgcggctttg gcgtgcgcga actcggcaag ccggcaccgg
     9361 tggatggcga cacccgcttc atgatcgcct ccaacaccaa ggcgctgacc acgctgatgc
     9421 tggccaagct ggtggatgcc ggcaaactgg actggaatgc ccgcgcggaa gacgtgtatc
     9481 cggccttcaa gctgggcgac gccgcgacca ccgacaaggt gctggtcaag cacctgatct
     9541 gcgcctgcac cggcctcccg cggcaggaca tggaatggct gttcgagggc gagaagcaga
     9601 cgcctgaaac ggtgatgagc acgctgggca cgatgcaacc caccagcggc ttcggcgaac
     9661 tgttccagta ctccaacccg atggccgcgg ccgccggcta catcggcggg caggtggcgt
     9721 atccgggcgg ggaactcggc gcgagctacg acaaggcgat gcaggccctg gtgttcgacc
     9781 cgctgggcat gcgcaacacc accttcgact acgcgaaggc gcagaccggc aactacgccg
     9841 cgccgcacgg cttcgacatc gaccacaagg tcgaggtcat ggacatgggg ctgaacgaca
     9901 ccatcgtggc ctcgcgtccc gccggcgcgg cctggggcac ggtcaacgac ctgctcaggt
     9961 acgtgcagat ggagatcgac cgcggcgtgt tgccggacgg cacgcgctac atcggcgagg
    10021 ccgccctgct gcagcggcgc gagccgcaga tcgcgctggg cgtggaccgc aactacgcga
    10081 tggcgctgat ggtggacaag agcgacggcg tcaccgtggt cgaccacggc ggcgacatgg
    10141 gcggcttcca ctccaacatg atgtggtggc cggagcagaa ggtcggcgcg gtgatcctga
    10201 ccaatgccga cgagggcgtg tacctgcgcg gcccgttcaa gcgccggctg atggagctga
    10261 tgttcgatgg cgaagcgcag gcgcaggcga ccgccgatgc caatgcgaag gcgaaccgcg
    10321 agggcttcga cgccttccgc aagctgctgc agatccccgg cgatgcggcc gcgctggccg
    10381 cgctggcccc gcgctaccgc aacgacgcgc tgggcgacat cgtggtcagc cgcagggatg
    10441 gcaaggcgcg catcgatgtc ggcgcgttct cgtctgagat cgtcacccag ccgcagcccg
    10501 acgggagcat ggcgttcgtc accatcgacc cggtggcggc cggcttccag ctggtccgcg
    10561 ccgacaagga cggcatgcgc aagctggtcg tccgcgacgg ccagcacgag tacgtgttcg
    10621 acgaagtgaa gtgaggttgc gccagctcgc tgcgtcggcg gcggggtggc tcagccggcc
    10681 ttcggggcct tgaagcccgc gcacggatcc gggcgatccg tcgccgggat gcgccgcgtc
    10741 tcatcgaata ccgccgcatc cgacgtcggc tccaggaacc cgaccgcatg cggcgccagg
    10801 ccgtcgcggc gcagccagaa cggcaccgcg atgtccttgc gcacgtggcc gttgccggcg
    10861 atcagcacca cgccggtcgc ggcatgcgtg cgcatggtgt ccgccattac cacgtcacgg
    10921 gcgatctgcg ccttcgccat cggcgagacc atcgcctcgg gcagcgcgcc gcaatggctg
    10981 tcgcgcactt cggcttcctg cgcggccagc agcggcgcgg gcagcgcatc gagctggtag
    11041 cgtgcgacga ggtccggcga cagggccgcg gtgaacccgc ccttgatcac cttcgacgcc
    11101 tcgctgcgcg acaggttggc cgcgcgaacc ggcagcccgt actgcagggc caaggtgagc
    11161 agcggcttgt agtactccca ggtccagccg gtcttcgcgg gcaccacctt ggcgaccacg
    11221 caatcggcat ccgcgcagtc gcgcatcgcc gcatccagcg ccgcctgctg gtcggtgtcg
    11281 aactgttcca tcgcgatgac cggccgccag cccgcggcca cctcgtcggc cagccatgcc
    11341 gcgcgcttcg cgtgcccggc ggcgctgtca tggacttcgc ccagcaacag cacccgcggc
    11401 gcggaggcgg ggggcgtgtg catgcaggcg ctgagcaggc aggccaggag ggcgcaggac
    11461 aaggggcgga tcggcatggg cgttggcata cggtggcgtc gggtcgatgc taacccacgc
    11521 gttacaatcg aaaccatttt tcgatcccgc cggaccgtcg catgagccgc accgccgaaa
    11581 ccctctcgca gccgcaatcc gagtccaccc cgctgcgctt cgtcaccgcc gccagcctgt
    11641 tcgacggcca cgacgccgcg atcaacatca tgcgccggct gatccaggcg cagggcgcgg
    11701 aagtggtgca cctgggccac aaccgctcgg tcgaggacgt ggtccgcgcc gcgttgcagg
    11761 aagacgccga cggcatcgcg ctgtccagtt accagggcgg ccacgtcgag tacctgaagt
    11821 acatggtcga catgctgcgc gagcgcgacg ccggccacgt caaggtcttc gccggcggcg
    11881 gcggcaccat cacccccgag gaaatccgcg agctcgaagc ctacggcgtc gagcgcatct
    11941 accaccccaa cgacggcatg aagatggggc tggtggagat gatcgaggac gtggtgacgc
    12001 gtgccggcga agggagggaa aaccggggaa accggggtca gagtcgactt tcctcgaagt
    12061 ccgacaaggc gcgtcaacac ctcgaaggga acgtcgactc tgaccccggt tttccttcgc
    12121 tctccgacga gatcgccatc ggccgcgtgc tctcggcgat cgaggacggc gattacgccg
    12181 aggccgaact ctcgcgcctg cgcaaggaat ggcaactggc cggcggcaag accccggtca
    12241 tcggcatcac cggcaccggc ggcgccggca agtcctcggt gaccgacgaa ctgctcaacc
    12301 gcttcctcgc cagcttcccg aagatgcaca tcgcggtgat cagcgtggat cccacccgcc
    12361 gccgcaccgg tggcgcgctg ctcggcgacc gcatccgcat gaactcgctg cgcagccatc
    12421 gcgtgtacat gcgctcgatg gccacgcgcc ggcagaacgt cgcgaccaat gcggtgttga
    12481 aggactgcat cggcttcctc aagtcgctgg gctatgacct ggtcatcgtg gagaccgccg
    12541 gcatcggcca gagcgactcg gagatcgtcg acctggtcga tttcccgatg tacgtgatga
    12601 ccagcgacta cggcgcggcg agccagttgg agaagatcga catgatcgac ttcgccgagc
    12661 tcatcgtgct caacaagtac gacaagcgcg gcgccgaaga cgcgttgcgc gacatccgca
    12721 agcagtggaa gcgcaaccgc gtcgcgttcc agaccgcaga cgccgacgtg ccggtgtatc
    12781 ccaccatcgc cagccagttc aacgaccccg gcatcagctg gatgttcgtg aatctatgcc
    12841 ggttgctccg ggaaaagtgg ggtcagagtc gactttccaa tatcggcaat tcgggtggtg
    12901 aggcaggggc ggggaaagtc gactctgacc ccacttttct tcgccccaac ctcgacacca
    12961 gcctcaagga accgcgcgcc accgtgctga tccccggcgc gcgcgtgcgc tacctggccg
    13021 agatcgccga gcagggccgc gccatcaacc gccgcatcga aacccaggcg gagatcgccg
    13081 accgcgcgca atcgctgtgg caggcgttgc aggaaatcgg cgatgccgcg ctgccgaagc
    13141 aactggacct gtatcccgac gacgcgctga ccagcgaagg cgaccgcagc ctgctcaccc
    13201 tccgccagcg ctacaacgac gccg
//
LOCUS       DAOOCX010000067        13063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_4397_length_13063_cov_9.340022, whole genome shotgun sequence.
ACCESSION   DAOOCX010000067 DAOOCX010000000
VERSION     DAOOCX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 13063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13063
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4397_length_13063_cov_9.340022"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PK399_07645"
     CDS             complement(<1..866)
                     /locus_tag="PK399_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005912141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HPW13029.1"
                     /translation="MSQWHLHDTTSNQRLGPMDTDAARAAAARNPSLLAWKDGMGDWL
                     PVRNIAELVDGVPGDAAPAPPPPPRNGGKGRADEIDFRIHGQEMQFVEIELDPGESAI
                     AEAGSLMFKDSAVQMDTVFGDGSASGQSGGFMDKLLSAGKRVITGESLFTTMYTHSGS
                     GKAKVAFAAPYPGTVMAMKLDEHGGKVICQKDSFLAGARGVQVGVHLQKKILTGLFGG
                     EGFIMQKLEGDGWVFIHAGGCVVERELAAGERIDVDTGCVVGYHATVNMDVQRVAGLK
                     SMFFGGEGVFLAT"
     gene            958..1473
                     /locus_tag="PK399_07650"
     CDS             958..1473
                     /locus_tag="PK399_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13030.1"
                     /translation="MTTLLRRIDAAPMRLAHALLVTALISLAGCATHAPRKVSSPPSM
                     LVGDFIDDYGIGHRIDAGEWLQRPDTRYRVVAWHPDAQYLIARNDAGNRSDAGTWTRI
                     DWLTLPGMPPWEWAFCLSAWDAATQADAERADIARRDTPKTGCNGYPFSRMRRVVEQE
                     ERQGREETGSE"
     gene            complement(1544..2092)
                     /locus_tag="PK399_07655"
     CDS             complement(1544..2092)
                     /locus_tag="PK399_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPW13031.1"
                     /translation="MTAPATIRFKATLRRPAHPKGATWRFLVLPAAASAKLPTRSMVS
                     VEGTLAGQPFQATLAPDGEGSHWLKVEKKLHEAAGIAAGGEVPLEIAPMAKEPEPKVP
                     ADLGEALAANAAAMATWKDVTTIARRDWIHWITSGKKAETRVKRIDVAIDKLASGNRR
                     ACCFDRSGRYSKGNMGPPEAAD"
     gene            complement(2117..2374)
                     /locus_tag="PK399_07660"
     CDS             complement(2117..2374)
                     /locus_tag="PK399_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HPW13032.1"
                     /translation="MDTTESSMTNLFLQLGLPAEKHEIAEFIRTHQLPEDVRVSEAAF
                     WSDGQRQFLREEWVEDADWAIIVDEFNEALHADAVAARASS"
     gene            complement(2465..4135)
                     /locus_tag="PK399_07665"
     CDS             complement(2465..4135)
                     /locus_tag="PK399_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPW13033.1"
                     /translation="MRRPTLLIAVLFATAQAPAAEPSRTDVAAFAARTLDANCDKAAP
                     GMAVLLARGDEVLYRGACGSANLELGVPLSADHVFRIGSVTKQFAAAAALKLVEAGKL
                     SLDDPLTKFVPGYPGGERVTVRMLLNHTSGLRSYTDVPGVMTGTPIMRDVTTAELIDT
                     FKAEKPDFAPGEGWHYNNSGYVLLGAVIEAASGKPWHVYLDETFFKPLGMRHTRYGNQ
                     QERVIPGHVDGYTFNEDHWARAGYLSMTQPHAAGALVSTVDDLLTWNRALHEGKVLQD
                     ASYRAMITPVGKAVENNYGFGISTGTLRGVPQLQHGGGIHGFSAYLLYLPGSDTSVAV
                     LYNADGVPPGVKAPSTIANLLAAQAIGNPYPEKKAIALDAATLKRYEGVYRVDKDVAR
                     VVRVVDGKLTSQRSGGSAFALIPVATDTFLFKESLSRITFERGAEGGIRAMRFFPEDE
                     GPGQLAPRTDEPLPGLRTAVALPPAVLARIAGTYAVPGATLAVTVEGAVAKVQLTGQP
                     AFEIFPESASRFFLKVVDAELVFAPETGRPASVTLHQGGRETVFQRTD"
     gene            complement(4186..5994)
                     /locus_tag="PK399_07670"
     CDS             complement(4186..5994)
                     /locus_tag="PK399_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010366861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HPW13034.1"
                     /translation="MRHSLLTAALLTALVAGCKAEAPTAPATSSATATAAASQADARF
                     AELSKRWLEGWLPLNPVAATQIGDHRFDAELDDVSAAGRQRALDFSKKLLAELDAIDA
                     KTLSRENQVDAAILRNQLRGDIWSTETLQAWAWDPQGYSGLAGSAIYNLMAREFAPLP
                     ERLKSATARMEKLPALFAQMRAEIVPARVPRIHAETVAKQHAGLLSLVDTFITPNADK
                     LQGEDRARLDAAVAGLRKAVAEHQEWLDKTLVPNAKGDFRIGQKLYDEKLQFSLNSEL
                     TRADIRQRADAEIERVRGEMYAVARTVLKDKPNAPELPDAPSEGQKQKAIEAALELAY
                     AERPARDKVVEYATQTTKDATAFVRAKDLVTVPDDPVKIILMPEFQRGVAVAYCDSPG
                     PLDKGLDTYYAISPIPDDWSSAQVDSFLREYNNRMLHLLSIHEAMPGHYLEGVHSAKH
                     PSTLRAVLRSGPFAEGWAVYTEDAMADAGYLDNDPLFRLVQLKFYLRAVANSILDQGV
                     HVDNWSKEQAMDLMVNKTFQQEREADGKWKRAQLTSTQLATYFVGAQEHFDMRKAVEA
                     KQDAGFSAKAYHDKVLSYGAPPVRFVRQLMLDEPIS"
     gene            complement(6111..6953)
                     /locus_tag="PK399_07675"
     CDS             complement(6111..6953)
                     /locus_tag="PK399_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPW13035.1"
                     /translation="MLHRPRLPASAFLALLLFQSSPPVVAQDAPSGVSTPEDMAKDLA
                     AVPCDDKDRLAAVRALYERAGATAPDITMDQHDNVENLVVTRKGASPETIVIGAHYDK
                     TAEGCGAIDNWTGQVALTHLYRTLRDIPLKKTLVFVAFGREEKGLVGSKAMTRDLDTT
                     EAAGTCAMINIDSLGLGPPQVADNMSSKKLARFAGELAKELEIPFAHASIRNANSDSS
                     AFVAKKIPAVTIHGLNKDWSSILHSKHDQAAKVNPVSVYLGYRLALAMIVRLDASACD
                     AYRQ"
     gene            complement(7012..7641)
                     /locus_tag="PK399_07680"
     CDS             complement(7012..7641)
                     /locus_tag="PK399_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13036.1"
                     /translation="MADAPQHKVSPNNPCPFLRMLVAQGLLDDRVATIGDATDVIERV
                     AATGEGSPRLPGIAIRLIALLANGLGPAILLDNGLNGVKLNQLRDGPFDKHGAGSRIL
                     DKRARVQPAELERLKDFASQKTTAGGGRELGLDAADIERMMDANYARAEGARRVIDRQ
                     IMNGEWPVLLDVMGKDGANGRYLSLREIRVLFEERRLPTRMGKRLRRRD"
     gene            complement(7701..8072)
                     /locus_tag="PK399_07685"
     CDS             complement(7701..8072)
                     /locus_tag="PK399_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPW13037.1"
                     /translation="MHYLGLAYFTPDRFAALPPDDVKAIVSQCPALDEAMRATGKVLV
                     SASLGDLEHWRTLLPGGGRTRVVDGPYTEAKEVVGGLFIIEADSQDEALRIASMHPAA
                     QIGEEGGWAVELIPLEFYMAR"
     gene            8206..8787
                     /locus_tag="PK399_07690"
     CDS             8206..8787
                     /locus_tag="PK399_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW13038.1"
                     /translation="MKRTATSSKQATHDRIVDAAARAIRRSGYNGTGVADIMKEAGLT
                     HGGFYAHFASRDAMLAEAADRAAGESVAMMERIAASLPPDQALPAMLEAYLSKPHLQQ
                     VETGCATAALASEMPRQTAEVRRAHTRRIKEMIDLVSRQLPDWGQPGAHERAMVTIAT
                     MVGTLALARAVDDDALSDALRHAALKQLTPTHS"
     gene            8855..9679
                     /locus_tag="PK399_07695"
     CDS             8855..9679
                     /locus_tag="PK399_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPW13039.1"
                     /translation="MNTQGSIALVTGASSGIGEATALRLAAAGYKVYGSNRRGTPSAH
                     PSFEMLALDVTDDASVDAAVAEVIRREGRIDLLVNNAGFGVAPAGAEESSIEQAKAIF
                     ETNFFGLVRMTRAVLPHMRGAGSGRIVNIGSVLGFLPMPFSALYSATKHAVEGYSESL
                     DHELRTRGIRVSVIEPAYTRTKFDANFLAADAMIDEYRDTRAALGKVLESVMASADEP
                     EVVAGVVLQAATDATPRLRYTAGGLANRLRLLRRFAPARMVDAGIRKDLRLDRSVS"
     gene            9676..10677
                     /locus_tag="PK399_07700"
     CDS             9676..10677
                     /locus_tag="PK399_07700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HPW13040.1"
                     /translation="MKALTFQRYGKSPGLGMTERPRPLPKPDELLVEVHAAGLNPIDN
                     MLMTGAFKPVVKLGLPATVGSDVAGVVVEVGSRVTRFKPGDAVFASVFDLDRGTLADY
                     VAVPEAVAAPKPANLDFVQAASVPMVGLTAWQALKERIGLRAGQKVFIPAGSGGIGTF
                     AIQLAKVLGASVATTTSTRNVDFVRGLGADEVVDYKQQQFQDVLRDYDAVLGTVKGEV
                     IEQSVGILKPGGRIASLVGPLDAAFARTRGLNVLLRIVFGLMSRKILRLAGKRGVAYS
                     FLFVHPDGGQLAEIGALLESGRLRPVIDKVFPFEQATDALAYLAQGRARGKVVVRLR"
     gene            10718..11182
                     /locus_tag="PK399_07705"
     CDS             10718..11182
                     /locus_tag="PK399_07705"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPW13041.1"
                     /translation="MNLIADVLRELGRTDLSGLEQLRALIASGKRPGISESLDFALTE
                     VDVGRAVFEGVPGHHAYNPIGMVHGGYAATLLDSACGCAVHSQLQAGQGYTTLELKVA
                     YHKAATSQTGLLRAEGRVLTMGRRAAFAEAKLTDASGRLYASATSTLLVIDG"
     gene            11226..11627
                     /locus_tag="PK399_07710"
     CDS             11226..11627
                     /locus_tag="PK399_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPW13042.1"
                     /translation="MRRRRRAFILNHMVQHNSAHFSATFAALADATRRGVLERLARGD
                     ASITDLADGFDMTLTGMKKHVGVLEQAGLVSTEKVGRVRTCRIGPCRLEEEMAWIARY
                     REVWDARFAALDDVLEDLKRQEQARGRKQRK"
     gene            11644..12102
                     /locus_tag="PK399_07715"
     CDS             11644..12102
                     /locus_tag="PK399_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPW13043.1"
                     /translation="MEKTSDREVVVTRTFDAPARLVFQAWSNPELFRTWWVPRSMGMT
                     LRSCEMDVRTGGSYRLVFGEDAANTFAFFGTYLEVVPEQRIVWTNEEGGDAGSITTVT
                     MEERDGKTLLVMREVYPTREALDEAGTGAQDATHETFAQLDALLAELAAG"
     gene            12225..>13063
                     /locus_tag="PK399_07720"
     CDS             12225..>13063
                     /locus_tag="PK399_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW13044.1"
                     /translation="MSHARLALLTLLALLAFAGNSLLCRAALAHTTIDAASFTLVRLL
                     SGAVVLGLLAWRRGTRGHARGNGWSAFALFAYAAAFSFAYLRLSAAAGALLLFGAVQA
                     TMIAHGLWSGERLRPLQLGGLVLAAGGLVGLVLPGLSAPPLAAALLMLAAGIAWGMYS
                     LRGRGAGDPLQVTAGNFLRAVPLAAVLALLMFRHITPDPTGLGYAVLSGALASGMGYA
                     IWYSALPLLTSVTAATVQLAVPVIAALGGVFLLGEPLTLRLLAASVAILGGVALVVMT
                     GAKR"
BASE COUNT         1953 a         4507 c         4410 g         2193 t
ORIGIN      
        1 gtggccagga acacgccttc gccgccgaag aacatgctct tcaggccggc cacgcgctgc
       61 acgtccatgt tgaccgtggc gtgataaccg accacgcagc cggtatcgac atcgatgcgt
      121 tcgccggcgg cgagttcgcg ttcgaccacg cagccgccgg catggatgaa cacccagcca
      181 tcgccctcga gcttctgcat gatgaagccc tcgccgccga acaggccggt gaggatcttc
      241 ttctgcaggt gcacgccgac ctgcacgccg cgcgcgccgg ccaggaagct gtccttctgg
      301 cagatgacct tgccgccatg ctcgtccagc ttcatcgcca tcaccgtgcc cggatatggc
      361 gcggcgaacg cgaccttggc cttgccgctg ccactgtgcg tgtacatggt ggtgaagagg
      421 ctctcgccgg tgatcacgcg cttgccggcg gacagcagct tgtccatgaa gccgccggac
      481 tggccgctgg cggaaccgtc gccgaacacg gtgtccatct gcaccgcgct gtccttgaac
      541 atcagcgagc ccgcttcggc gatggcggat tcgcccggat ccagttcgat ctcgacgaac
      601 tgcatttcct gtccgtggat gcggaagtcg atctcgtcgg cgcggccctt cccgccattg
      661 cgcggcggcg gcggcggcgc aggcgcggca tcgcccggca cgccatcgac cagttcggcg
      721 atgttgcgca ccggcagcca gtcgcccatg ccgtccttcc aggccagcag cgacgggttg
      781 cgcgcggccg cggcgcgcgc ggcatcggtg tccatcgggc caaggcgctg gttgctggtg
      841 gtgtcgtgca ggtgccactg gctcatcgcg aactccgttg cggggggaat ggcgcggagt
      901 ctaagccgcg cgcgatgacg ggcaaggtgc cggaagtcgc gcttgatccg cgctttaatg
      961 accacattgc ttcgcaggat cgatgccgcg ccgatgcgcc ttgcccacgc cctgctggtc
     1021 accgcactga tttccctcgc cggctgtgca acgcatgcgc cgcgcaaggt ctcgtcgcca
     1081 ccttcgatgc tggttggcga cttcatcgac gactacggca tcggccaccg catcgatgcc
     1141 ggcgaatggc tgcaacgacc ggacacgcgc taccgcgtgg tcgcctggca tcccgacgcg
     1201 caatacctga tcgcccgcaa cgatgccggc aatcgcagcg atgccggcac gtggacgcgc
     1261 atcgactggc tcaccttgcc cggcatgccg ccctgggaat gggcgttctg cctgagcgca
     1321 tgggacgccg cgacgcaagc cgatgccgag cgcgccgaca tcgcacgacg cgatacgcca
     1381 aagacgggtt gcaacggcta tccgttctcg cggatgcgtc gcgtggtgga gcaagaggaa
     1441 aggcagggaa gagaggaaac ggggtcagag tgaatttccc gccgagcaat tcagatcgct
     1501 cggaagaact gatcaggaaa cttcactctg accccatttc cttttaatcg gcggcctccg
     1561 gcggccccat gttgcccttg ctgtacctgc cggaacggtc gaagcagcag gcgcggcgat
     1621 tgccactggc cagcttgtcg atggccacgt cgatccgttt cacccgggtt tccgccttct
     1681 tgccggaggt gatccagtgg atccagtcgc gccgcgcgat ggtggtgacg tccttccagg
     1741 tcgccatcgc ggcggcgttc gcggccagcg cttcacccag gtcggcgggc accttcggtt
     1801 ccggctcctt cgccatcggc gcgatctcga gcggcacctc cccgcccgcc gcgatgccgg
     1861 cggcttcgtg caacttcttc tcgaccttca accagtgact gccctcgcca tccggcgcca
     1921 gcgtggcctg gaacggctgc ccggccagcg tgccttccac gctcaccatg ctgcgggtcg
     1981 gcagcttcgc gctggcggcc gccggcagga ccaggaaccg ccacgtcgcg cccttgggat
     2041 gcgcgggccg acgcagcgtg gccttgaagc ggatcgtcgc aggcgcggtc atgcgcagac
     2101 gatagcggac gcgggctcag gaggaagcgc gcgccgccac cgcatccgcg tgcagcgctt
     2161 cgttgaattc gtcgacgatg atcgcccagt cggcatcctc cacccactcc tcgcgcagga
     2221 actgccgctg gccatcgctc cagaaggccg cttcggagac gcgcacgtcc tccggcaact
     2281 gatgcgtgcg gatgaactcg gcgatctcgt gcttctccgc cggcaggccc agctgcagga
     2341 acaggttggt catgctgctc tcggtggtat ccacgcgcgt ttcccggtga tgaggtgatg
     2401 ccggagtgtc gaccacgcga tgtcaacgcg gcatcgcgac gcgcgcaagg cccctgctgc
     2461 gggctcagtc ggtacgctgg aacaccgtct cgcgcccgcc ctggtgcagg gtcaccgatg
     2521 ccgggcgtcc cgtttccggc gcgaacacca gttccgcatc gacgaccttc aggaagaacc
     2581 gcgacgccga ctccgggaag atctcgaacg ccggctgccc ggtcagctgc accttggcca
     2641 cggcaccttc cacggtcacc gccagcgtcg cccccggcac cgcgtacgta ccggcgatcc
     2701 gtgccagcac cgcaggcggc agcgcgacgg cggtgcgcag gcccggcagc ggttcgtcgg
     2761 tccgtggcgc cagttgcccg gggccttcat cctccgggaa gaagcgcatc gccctgatcc
     2821 cgccctccgc gccgcgctcg aaggtgatgc gcgacaggct ttccttgaac aggaaggtat
     2881 ccgtggcgac cgggatcagc gcgaacgccg atccaccgct gcgctgcgag gtcagcttgc
     2941 cgtcgaccac ccgcacgacc cgcgccacgt ccttgtcgac ccgatacacg ccttcgtacc
     3001 gcttcagcgt ggcggcatcg agcgcgatcg ccttcttctc cggatacggg ttgccgatcg
     3061 cctgcgccgc aagcaggttg gcgatcgtgc tgggggcctt cacccccggg ggcaccccat
     3121 ccgcgttgta gagcaccgcc acgctggtat cgctgccggg caggtacagg agataggcgc
     3181 tgaagccgtg gatgccaccg ccgtgctgca gctgcggcac cccgcgcagg gtgccggtgc
     3241 tgatgccgaa accgtagttg ttctccaccg ccttgccgac cggcgtgatc atcgcgcggt
     3301 aactggcgtc ctgcagcacc ttgccctcgt gcagcgcgcg gttccacgtg agcaggtcgt
     3361 cgacggtcga caccagcgca ccggccgcat gcggctgggt catgctgagg tagccggcgc
     3421 gcgcccagtg atcctcgttg aaggtgtaac cgtccacgtg gcccgggatc acccgctcct
     3481 gctggttgcc gtagcgggtg tggcgcatgc ccagcggctt gaagaaggtt tcgtccaggt
     3541 agacgtgcca cggcttgccg ctggccgctt cgatcactgc acccagcagc acgtagccgg
     3601 aattgttgta gtgccagccc tcgccggggg cgaagtcggg cttctcggcc ttgaaggtgt
     3661 cgatgagttc cgccgtggtg acgtcgcgca tgatcggggt gccggtcatg acgcccggga
     3721 catcggtata gctgcgcaag cccgaggtgt ggttgagcag catgcgcact gtcacccgct
     3781 cgccgcccgg atagccgggc acgaatttcg tcagcggatc gtcaagcgac agcttgccgg
     3841 cctcgaccag tttcagcgcc gcagccgccg cgaactgctt ggtgaccgac ccgatccgga
     3901 acacgtggtc ggcggacagc ggcacgccca gctcgaggtt ggcgctgccg caggcgccgc
     3961 ggtagagcac ctcgtcgccg cgtgccagca agaccgccat tcccggcgcg gccttgtcgc
     4021 agttggcgtc cagcgtgcgc gcggcgaacg ccgccacgtc ggtcctggag ggctcggcgg
     4081 caggcgcctg cgcggtggcg aagagcacag cgatcagcag ggtcgggcga cgcatggtgg
     4141 gttccgatgg gatgtcgtgg gatgtcgcgg cgtggcgcgc ctgctttagc tgatcggttc
     4201 gtccagcatc agctggcgca cgaagcgcac cggtggcgcg ccataggaca gcaccttgtc
     4261 gtggtaggcc ttggcgctga agcccgcgtc ctgcttcgcc tccaccgcct tgcgcatgtc
     4321 gaagtgctcc tgcgcaccga cgaagtacgt ggccagctgc gtcgaggtca gctgcgcgcg
     4381 cttccacttg ccgtctgctt cgcgttcctg ctggaaggtc ttgttgacca tcaggtccat
     4441 cgcctgttcc ttgctccagt tgtcgacgtg cacgccctgg tcgaggatcg aattggccac
     4501 cgcgcgcagg tagaacttca gctgcaccag gcggaacagc ggatcgttgt cgaggtagcc
     4561 ggcatcggcc atggcgtcct cggtgtacac cgcccagcct tccgcgaacg ggcccgagcg
     4621 cagcaccgcg cgcagggtgg agggatgctt ggccgaatgc acgccttcca ggtaatggcc
     4681 gggcatcgct tcgtggatcg acagcaggtg cagcatgcgg ttgttgtatt cgcgcaggaa
     4741 cgaatcgacc tgcgcgctgc tccagtcgtc cgggatcggc gagatcgcgt agtaggtgtc
     4801 caggcccttg tcgagcggac ccggcgagtc gcagtacgcc accgccacgc cgcgctggaa
     4861 ttccggcatc aagatgatct tcactggatc gtccggcacc gtgaccaggt ccttggcgcg
     4921 cacgaaggca gtggcatcct tggtggtctg ggtggcgtat tcgaccacct tgtcgcgcgc
     4981 ggggcgctcg gcatacgcga gctccagcgc ggcttcgatc gccttctgct tctgtccttc
     5041 gctgggcgca tccggcagct cgggcgcatt cggcttgtcc ttcagcaccg tgcgcgcgac
     5101 cgcgtacatc tcaccgcgca cgcgctcgat ctccgcatcc gcgcgctggc ggatgtcggc
     5161 gcgggtcagt tccgaattca gcgagaactg cagcttctcg tcgtagagct tctggccgat
     5221 gcggaagtcg cccttcgcat tcggcaccag ggtcttgtcc agccattcct ggtgttcggc
     5281 gacggccttg cgcaggcccg ccaccgcggc atcgagccga gcgcggtctt cgccctgcag
     5341 cttgtcggcg ttgggggtga tgaaggtatc gaccaggctg agcaggccgg catgctgctt
     5401 cgccactgtt tccgcatgga tcctgggcac ccgcgccggg acgatttccg cgcgcatctg
     5461 cgcgaacagg gccggcagtt tttccatccg cgccgtcgcc gacttcaggc gctccggcag
     5521 cggcgcgaac tcgcgcgcca tcaggttgta gatcgcgctg ccggccaggc cgctgtagcc
     5581 ctgcggatcc caggcccacg cctgcagggt ttcggtggac cagatgtcgc cgcgcagctg
     5641 gttgcgcagg atcgcggcat ccacctggtt ctcgcgcgag agcgtcttcg catcgatcgc
     5701 atccagttcc gccagcagct tcttgctgaa atcgagtgca cgctggcggc cggccgcact
     5761 gacatcgtcg agctcggcat cgaagcggtg gtcgccgatc tgggtggccg ccaccgggtt
     5821 cagcggcagc cagccttcca gccagcgctt ggagagttcg gcgaaccgtg catccgcctg
     5881 cgaggccgca gccgttgcgg tcgccgatga tgtcgcgggc gcggtcggcg cctcggcctt
     5941 gcagccagcg acgagcgccg tcagcagggc ggcggtgagc agggaatggc gcatgcgggc
     6001 gagtctccgg ggatggaagt ggccaagctt aggggattcg cggcggcggg cccgatacag
     6061 cgcttgcggg cgacgcgcca ccgccgcgcg gcggtcgatc gcgatcgcgg ctattgccga
     6121 taggcgtcgc aggccgaggc atccagccgc acgatcatcg ccagcgccag ccgatagccc
     6181 aagtacacgc ttaccggatt caccttggcc gcctggtcat gcttgctgtg caggatcgag
     6241 gaccagtcct tgttcaagcc gtggatcgtc accgccggga tcttcttcgc cacgaatgcg
     6301 ctggaatccg aattcgcgtt gcggatgctg gcgtgcgcga acgggatctc cagttccttc
     6361 gccagctcgc cggcgaaacg ggccagcttc ttgctggaca tgttgtcggc gacctgcggc
     6421 gggcccaggc ccaggctgtc gatgttgatc atcgcgcagg tacccgcggc ctccgtggtg
     6481 tcgaggtcgc gcgtcatcgc cttcgagccg accaggccct tctcttcgcg cccgaaggcg
     6541 acgaacacca gcgtcttctt gagcgggatg tcgcgcagcg tccggtagag gtgggtcagc
     6601 gcgacctggc cggtccagtt gtcgatcgcc ccgcagcctt ccgcggtctt gtcgtagtgc
     6661 gcgccgatca cgatggtctc gggcgacgcg cctttcctgg tgacgaccag gttctcgacg
     6721 ttgtcgtgct ggtccatggt gatgtcgggc gcggtcgcgc cggcgcgttc gtacaacgcc
     6781 ctgaccgcgg ccaggcgatc cttgtcgtcg cagggcacgg ccgcgaggtc cttcgccatg
     6841 tcctcgggcg tggacacacc ggaaggcgcg tcctgcgcca ccaccggcgg ggaggactgg
     6901 aagagcagca gggcgaggaa cgcggaagcg ggcaagcgcg ggcgatgcag catggggatc
     6961 cgggcggtgg cgaggtccga ggattgtgac ccggacgacc gcgcatgcgc gctagtcgcg
     7021 acgccgcaac cgcttgccca tccgcgtggg gaggcgacgc tcctcgaaca gcacgcggat
     7081 ctcgcgcagg ctcaggtagc gaccgtttgc gccgtccttg cccatgacat cgagcagcac
     7141 cggccattcg ccattcatga tctgtcggtc gatgacccgg cgcgcgcctt cggcgcgtgc
     7201 gtagttcgca tccatcatcc gttcgatgtc ggctgcgtcg agcccgagtt cgcggccgcc
     7261 gcccgcggtg gtcttctggc tggcgaagtc cttgaggcgc tccagttccg cgggctgcac
     7321 gcgggcgcgc ttgtccagga tccgcgaacc cgcgccgtgc ttgtcgaacg ggccatcgcg
     7381 cagctggttg agcttgaccc cgttgaggcc gttgtcgagc agtatggccg ggccaagccc
     7441 gttcgcgagc aaggcgatca gccggatcgc gatccccggc aagcgcgggc tgccttcgcc
     7501 cgtcgcggcg acgcgttcga tcacgtcggt ggcatcgccg atggtcgcca cccgatcgtc
     7561 cagcaagccc tgcgccacca gcatgcgcag gaacgggcag ggattgttgg gtgagacctt
     7621 gtgctgcggt gcgtcggcca tggcggtcac tcggggggcg gacggggagc gtacgtgcgg
     7681 tggcgtgatg gcggcgcggg tcaacgcgcc atgtagaact ccagcgggat cagttccacc
     7741 gcccagccgc cttcctcgcc gatctgcgcg gccggatgca tcgacgcgat gcgcagcgct
     7801 tcgtcctggc tatcggcctc gatgatgaag agcccgccca cgacttcctt cgcctcggtg
     7861 tacggcccat cgacgacgcg ggtcctgccg ccgcccggca gcaaggtgcg ccagtgttcg
     7921 agatcgccca gcgaagccga caccagcacc ttgccggtcg cgcgcatcgc ctcgtccagc
     7981 gccgggcatt ggctgacgat ggccttcacg tcgtccggcg gaagcgcggc gaacctgtcg
     8041 ggggtgaagt aggcgaggcc gaggtagtgc atggggtgtc tccggatgga atgcctggac
     8101 gacgatgccg caggggcgga atcgacacgg cccattgtcc tgcgctcggc gcccgcgcgt
     8161 gccgcacttg caccgccggg aatccgtata tatgatggcc gtcatatgaa acgcaccgcc
     8221 acgtcctcca agcaagccac ccacgaccgc atcgtcgatg ccgccgcccg cgcgatccgg
     8281 cgcagcggct acaacggcac cggcgtggcc gacatcatga aagaggccgg cctgacccat
     8341 ggcggcttct acgcccactt cgcgtcccgc gacgcgatgc tggccgaggc cgccgaccgg
     8401 gcggcgggcg agagcgtggc gatgatggaa cgcatcgccg ccagcctgcc gccggaccag
     8461 gcgctgccgg ccatgctgga ggcctacctg tcgaagccgc atctccagca ggtcgagacc
     8521 ggctgcgcca cggcggcgct ggcctccgag atgccgcgcc agactgcgga ggtccggcgc
     8581 gcgcataccc ggcggatcaa ggaaatgatc gacctcgtct cccgccagct gcccgactgg
     8641 gggcaacccg gcgcgcacga gcgggcgatg gtcaccatcg cgaccatggt cggtaccctc
     8701 gccctggcgc gcgcggtcga cgacgacgcc ctgtccgatg cgctgcgcca cgccgcgctg
     8761 aagcagctga cccccaccca ttcctgatcc ggcacctggc cgggtctttc ctgtcgcgca
     8821 ctgatatgac gatcatcata catcggagtc tcccatgaac acccaaggat ccatcgcact
     8881 ggtcaccggc gcctcgtccg gcatcggcga ggccaccgcg ctgcggctgg ccgccgcggg
     8941 ttacaaggtc tacggcagca accgccgtgg cacgccatcg gcacatccct cgttcgagat
     9001 gctcgcactg gacgtaaccg acgacgcctc ggtcgacgcc gccgtcgccg aggtcatccg
     9061 ccgcgagggc cgcatcgacc tgctggtcaa caacgccggc ttcggcgtcg cgcccgccgg
     9121 ggccgaggag agttcgatcg agcaggccaa ggcgatcttc gaaaccaact tcttcggcct
     9181 ggtgcggatg acccgcgcgg tgctgccgca catgcgcggt gccggcagcg gccgcatcgt
     9241 caacatcggc tcggtgctcg gcttcctgcc gatgccgttc agtgcgctgt attccgcgac
     9301 caagcatgcg gtcgaaggct attcggaatc gctcgaccac gagctgcgca cgcgcggcat
     9361 ccgcgtctcg gtgatcgagc ccgcgtacac gcggacgaag ttcgatgcca acttcctggc
     9421 ggccgacgcg atgatcgacg aataccgcga cacccgcgcc gccctgggca aggtgctcga
     9481 gtcggtgatg gccagcgccg acgagccgga ggtcgtcgcc ggcgtggtgc tgcaggcggc
     9541 caccgacgcc actccgcggc ttcgctacac cgcgggcgga ctcgccaacc gcctgcgcct
     9601 gctgcgccgg ttcgcgccgg cacgcatggt cgatgccggc atccgcaagg accttcgcct
     9661 cgaccggagc gtgtcatgaa ggcgctcacc ttccagcgct acggcaagtc gcccggcctg
     9721 ggcatgaccg agcggccgcg cccgctgccg aagccggacg aactgctggt cgaggtccat
     9781 gccgccggcc tgaatccgat cgacaacatg ctgatgaccg gcgcgttcaa gccggtggtg
     9841 aaactcggcc tgccagccac cgtgggcagc gacgtggccg gcgtggtggt cgaagtcggc
     9901 agccgggtca cccgcttcaa gccgggcgat gcggtgttcg ccagcgtctt cgacctggac
     9961 cgcggcaccc tcgcggacta cgtggcggtg ccggaggccg tggccgcccc caagcccgcc
    10021 aacctcgact tcgtgcaggc ggcttcggtg ccgatggtcg ggctcaccgc atggcaggcg
    10081 ctgaaggaac gcatcggcct gcgcgcgggg cagaaggtat tcattcccgc cggctccggt
    10141 ggcatcggca ccttcgcgat ccagttggcg aaagtgctgg gtgccagcgt cgccaccacc
    10201 accagcaccc gcaacgtgga cttcgtgcgc ggcctgggtg ccgacgaggt ggtggattac
    10261 aagcagcagc aattccagga cgtgctgcgc gactacgacg cggtgctcgg caccgtgaag
    10321 ggcgaggtca tcgagcaatc cgtcggcatc ctcaagccgg gcggccgcat cgcctcgctg
    10381 gtgggaccgc tggacgcggc cttcgcccgc acgcgtgggt tgaacgtgct gctgcggatc
    10441 gtattcggcc tgatgagccg caagatcctg cgcctggcgg gcaaacgtgg tgtcgcctac
    10501 tccttcctgt tcgtgcaccc cgatggcggg caactcgcgg agatcggtgc gttgctcgaa
    10561 tcggggcgtc tccgtccggt gatcgacaag gtgttcccgt tcgagcaggc cacggacgcg
    10621 ctggcctacc ttgcccaggg acgtgcccgt ggcaaggtcg tcgtccgcct gcgatgaacc
    10681 gcgcctcgca gtcacaaccg caacctggag cagtgccatg aacctgatcg ccgacgtcct
    10741 ccgcgagctt ggcaggaccg acctcagcgg gctggaacaa ctgcgcgcgc tgatcgcctc
    10801 cggcaagcgc cccggcatct cggaatcgct cgacttcgcg ctgaccgagg tcgatgtcgg
    10861 ccgcgcagtg ttcgaaggcg tgcccggcca ccacgcctac aaccccatcg gcatggtcca
    10921 cggcggttac gccgcgaccc tgctggattc cgcctgcggc tgcgcagtgc attcgcaact
    10981 gcaggcgggc cagggctaca ccacgctgga actcaaggtc gcctaccaca aggccgcgac
    11041 cagccagacc ggcctgctgc gcgcggaagg ccgcgtgctg acgatgggac gccgcgcggc
    11101 gttcgccgag gccaagctga cggatgcgag cggccggctg tatgcgtcgg ccacctcgac
    11161 gctgctggtc atcgacgggt gagtcccgca tcccaggcac gctgtcgctg atcgcataag
    11221 agcgcttgcg ccgtcgccga cgggctttca tactgaacca tatggttcag cataattctg
    11281 cccatttcag cgccaccttc gccgcgctgg ccgatgccac ccgccgtggc gtgctggaac
    11341 ggctcgcgcg cggcgacgcg tccatcaccg acctcgccga cggcttcgac atgaccctga
    11401 ccggcatgaa gaagcacgtc ggcgtgctcg agcaggccgg cctggtgagc acggagaagg
    11461 tcggccgcgt gcgcacctgc cgcatcgggc cgtgccgcct cgaagaggaa atggcctgga
    11521 tcgcgcgtta ccgcgaggtg tgggatgcgc gcttcgccgc gctggacgat gtactcgaag
    11581 acctcaaacg acaggagcag gcccgtggtc gcaaacaacg caagtgaatc ccgccgcacg
    11641 acggtggaga agacgtccga tcgcgaagtc gtcgtcaccc ggacattcga tgcacccgca
    11701 aggctggtgt tccaggcgtg gtcgaacccc gagctgttcc gcacatggtg ggtgccgcgc
    11761 tccatgggca tgaccctgcg ctcgtgcgag atggacgtgc gcaccggcgg cagctatcgc
    11821 ctggtcttcg gcgaggacgc cgccaacacg ttcgccttct tcggcaccta cctggaggtg
    11881 gtgcccgaac aacgcatcgt ctggaccaac gaggaaggcg gcgatgcggg atcgatcacc
    11941 acggtgacga tggaagagcg cgacggcaag accctgctgg tgatgcgcga ggtctatccc
    12001 acccgcgaag cgctggacga agccggcacc ggcgcgcagg acgccacgca cgagacgttc
    12061 gcccagctcg acgccttgct ggccgagctc gccgcgggct gatcgctgcg gcgtcgatgc
    12121 aacggagccc ggccacgcgc cgggcttcgc gccttacatt gcacgccgag gcatcgtgcc
    12181 gccgcgcgat gcgaaccgag acatcccccg aagccggccc acgcatgtcg cacgcacgcc
    12241 tcgccctgct gacactgctc gcgttgctcg ccttcgcggg caattcgctg ctgtgccgcg
    12301 cggcgctggc gcacaccacg atcgatgcgg ccagcttcac cctggtgcgc ctgctgtccg
    12361 gtgcggtggt gctgggattg ctggcgtggc ggcgagggac tcgcggacat gcacgcggca
    12421 acggatggtc cgccttcgcg ctgttcgcct acgccgcggc gttctcgttt gcgtacctgc
    12481 gactcagcgc ggccgcgggg gcgctgctgc tgttcggcgc cgtgcaggcc accatgatcg
    12541 cgcacggcct gtggtcgggt gaacgcctgc ggccgctgca actcggcggt ctggtgctcg
    12601 ccgcaggcgg cctggtcggc ctggtgttgc cgggcctgtc cgcaccacca ttagcggccg
    12661 cattgctgat gctggcggcc ggcatcgcct ggggcatgta ttcgctgcgc ggtcgcggcg
    12721 cgggcgatcc gttgcaggtc accgccggca acttcctgcg tgcggtgccg ctagccgcag
    12781 tgctggcctt gctgatgttc cggcacatca cccccgaccc gacgggactg ggctacgcgg
    12841 tgctgtccgg cgcgctggcc tcgggcatgg gctatgcgat ctggtacagc gcactgccgt
    12901 tgctgacatc ggtgaccgcc gcgaccgtgc aactcgcggt gccggtgatc gccgcgctgg
    12961 gcggcgtgtt cctgctcggc gaaccactga ccctgcggct gctggcggcg tcggtggcga
    13021 tcctgggcgg tgtggcgctg gtggtaatga cgggcgcgaa gcg
//
LOCUS       DAOOCX010000068        12525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_4667_length_12525_cov_8.722534, whole genome shotgun sequence.
ACCESSION   DAOOCX010000068 DAOOCX010000000
VERSION     DAOOCX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12525
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4667_length_12525_cov_8.722534"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PK399_07725"
     CDS             <1..503
                     /locus_tag="PK399_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW13045.1"
                     /translation="EIRYFDIKGAKTGLLSKAMTAPDGMVRIPLNESSDPKSQINEYL
                     DVYGGEGIQHIACFTDDIYGTVEKMRETGIEFLDTPDTYFDVIDQRIPAHGEDVERLR
                     ANKILIDADPETKQRKLLQIFTLNALGPIFFEIIQRKGNEGFGEGNFQALFESIERDQ
                     MKRGVL"
     gene            570..1874
                     /gene="hmgA"
                     /locus_tag="PK399_07730"
     CDS             570..1874
                     /gene="hmgA"
                     /locus_tag="PK399_07730"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HPW13046.1"
                     /translation="MTIESNGYQSGFGNECASEAIAGTLPQGRNSPQVVAHGLYAEQV
                     SGTAFTAPRHQNRRSWLYRIRPAAMHGTFALLEHDGFHNDFDTGPVTPDQLRWNPLPL
                     PDAPTDFIDGLLTVAGNGAASAGVGIGIHLYAANRDMQGRFFYDADGELLIVPQQGRL
                     HIETELGVLDVEPQEIAVIPRGIRFRVALPDGEARGYVCENFGAFMRLPDLGPIGSNG
                     LANPRDFLTPNAAFEDLEGSFELIAKFQGHLWRADIGHSPLDVVGWHGNHAPYKYDLR
                     TFNTIGSISYDHPDPSIFLVLTSPSDSPGVGNMDFVIFPPRVLVAQGTFRPPWFHRNI
                     ASEFMGLVHGVYDAKADGFAPGGCSLHNCMTGHGPDAATFDKASAADTSKPDYVDGTM
                     AFMFESRSVIRPTRQALDAAHRQRDYQACWAGLRKHFVAPQG"
     gene            1949..2602
                     /locus_tag="PK399_07735"
     CDS             1949..2602
                     /locus_tag="PK399_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13047.1"
                     /translation="MRTLLSIALLAALAGCGQGGTPDATPTAPVDAAPVADTPPTAIA
                     TDTPASTDASAGGSLRVDRPADGTITFKGFGPANFGATQEDVRMAWGGDLGDGKPDEA
                     GGCYYLVPQPVGTDGFRTAFMIEGDKFVRIDVRRDDVAAPGGGKVGMRKAQVAALYAG
                     IEEGPHKYTDGQYLRVKDPAGGSGVMVFETDGAGDDATVTEWRIGIPPQVDYVEGCS"
     gene            2645..3889
                     /gene="nhaA"
                     /locus_tag="PK399_07740"
     CDS             2645..3889
                     /gene="nhaA"
                     /locus_tag="PK399_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005393226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HPW13048.1"
                     /translation="MDTQAPLPKRALRALNHFLQWESSGGILLIVAALVALALANSPL
                     AGAYGEFRELPVAVQFGALKIAKPLLLWINDGLMAIFFLLVALELKREALSGQLSSFS
                     QLSLPVVCALAGVAVPAGIYAWLNRGDAVAMQGWAVPAATDIAFALGILSLLGSRVPV
                     AMKLLLSTIAVIDDLAAIVIIALFYTSDLSVVALGWAGAAIGLMVVLNRRGVQALTPY
                     LLLGTIVWVAVLKSGVHATLAGVATGLLIPHVNRKDAIDDESQHSPLEHLEHALHPWV
                     AYLILPVFAFANAGLALGGFALDDLLTPVPLGVALGLVAGKPVGVVLAALLARATGFS
                     RFPEGMDWKAMVGLGLLCGIGFTMSLFIASLAFGDDAGLFPSAVLGVLCASLVSALAG
                     LAWLWATLPRAPAAAGHPPRAD"
     gene            3899..5323
                     /gene="nhaD"
                     /locus_tag="PK399_07745"
     CDS             3899..5323
                     /gene="nhaD"
                     /locus_tag="PK399_07745"
                     /inference="COORDINATES: protein motif:HMM:NF038006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HPW13049.1"
                     /translation="MIRRAPRFPRLALLPMAALPTTAWAMAPAQDFTAHWSGLVAVGI
                     FVAAYVLVIAEESTGLRKSQPVMLAAGAIWAVLAIASARAGLSDAVHEAVRDYLLEFA
                     ELLLFLLAAMTYVNAMSERGLFEVLRVWLLRRGLGYRALFWTTGMLAFCMSPLIDNLT
                     TALVLCAVVLAVGRDSPKFVALACINIVVAANAGGAFTPFGDITTLMVWQAGKVPFAS
                     FFALFLPALVNWGVPALCMHAAVPGGTPVLTDAQARLRRGGLVIVGLFGLTIAMAVGF
                     HQFLHLPPFLGMMTGLALLKLYGWHLTRAATAQQAAEFAREGAPGDVDAFDSYEQVAR
                     AEWDTLLFFFGVIMCVGALGYCGYLALLSQQFYGGLGPTTANVLVGLLSAVLDNIPMM
                     VAVLQMAPAMDTGQWLLVTLTAGVGGSLLSIGSAAGVALMGQAHGQYTFFSHLKWTWA
                     IALGYAASVGVHLWLNARYFTGVA"
     gene            5361..6233
                     /locus_tag="PK399_07750"
     CDS             5361..6233
                     /locus_tag="PK399_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar epimerase"
                     /protein_id="HPW13050.1"
                     /translation="MKTALVFGASGQVGLPLLDRLGDAGWRIYAVSRQARSDTPGRHW
                     LQGDFARMPGLPRDVDALFSCGPLDLFARWYATADIAVPRVVAFGSTSATSKHASGDD
                     GERDVARRLLGAEAAVFEASEQRGAGATMLRPTLVYGAGRDATLTRIVGMARRWGRFV
                     LPRRADGLRQPVHVQDLADAAFGASLAPATPGRSYDLPGGETLAYREMVRRVLACLDP
                     PLPLLELPMPLFRAALAAAQARGIARDLTPAAVARMREDLAFDAAPARRDFGYSPRGF
                     RPEARMFDATDRRD"
     gene            complement(6237..7139)
                     /locus_tag="PK399_07755"
     CDS             complement(6237..7139)
                     /locus_tag="PK399_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW13051.1"
                     /translation="MTATGLRGRDLVLVLLICLFWATNFLTSAHALREVPPFLFTAMR
                     MGLLAVLLVGFMRVPPRAQWKRLFAVAMLNGVLHFGTSFLALKLAGNLSSPAIVTQVY
                     VPMSVLLAWWWLGERFGWRTGVGVAISFAGVLVLGFDPMVLAQPAALFVMMGSSLMVA
                     IGTVLMRGLKDVRMVDQQGWTAVLSLLPLLAISALFEPGSFAALRDASWIAWGAAVYA
                     AVFSSLLGHGVFYWLVQRHPVATVTPYLLLTPVLAVLLGILFWGDRPGLALWIGGAMV
                     LGGVLVVALRAGVRQRPLPPAEEL"
     gene            complement(7158..7658)
                     /locus_tag="PK399_07760"
     CDS             complement(7158..7658)
                     /locus_tag="PK399_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008575562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HPW13052.1"
                     /translation="MNATVQHEAGNRPIDHWFTHYSGDHVNPTNQRIHVFAVPLILWS
                     VIGLLWCIPVPGTLFRAGLWAALAMFGGWMFYYRASRAIGFGMLAVFVAMAWLTRWLH
                     DTLGTPGLFKLALGVFVVAWIAQFIGHSKLYEGKKPSFFTDLKYLLIGPAWVLAKAYR
                     KLGIRW"
     gene            complement(7716..8612)
                     /locus_tag="PK399_07765"
     CDS             complement(7716..8612)
                     /locus_tag="PK399_07765"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPW13053.1"
                     /translation="MATTLLQSDLPGLTLRHRGKVRDVFDLGNDTLLMVATDRLSAFD
                     VVLPDPIPGKGEMLCQISNFWFDKTRHIIANHLTGIDVASVLPAGVDPALYAKRAVVT
                     KKLTPVPVECIARGYVIGSGWKDYQRSGAISGIDLPPGLQQAQQLDTPIFTPSTKAAV
                     GDHDENIDFATAVDLVGRERAEQVRDATLALYAFARDYAATRGIILADTKFEFGTDAD
                     GKLYVMDEVLTPDSSRYWPADDYRVGTSPPSYDKQFVRDYLETLDWDKTPPGPRLPAE
                     VIARTRAKYAEALDRLAGISVD"
     gene            8753..9079
                     /locus_tag="PK399_07770"
     CDS             8753..9079
                     /locus_tag="PK399_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPW13054.1"
                     /translation="MKRWYGKFFGLIAGWLLFRHPAGGLLGLLIGHAFDADWFKSARD
                     NPYGVLQVSSDASDAEVERAYRRLISQYHPDRLAGAAPQLQRQAEEKAREINTAYDRI
                     KTLRKT"
     gene            9082..9780
                     /gene="rpe"
                     /locus_tag="PK399_07775"
     CDS             9082..9780
                     /gene="rpe"
                     /locus_tag="PK399_07775"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPW13055.1"
                     /translation="MSPAPPLDCVIAPSILSANFAKLGEEVDNVLAAGADWVHFDVMD
                     NHYVPNLTIGPLVCEALRAHGVTAPIDVHLMVEPVDRIVPDFAKAGASIISFHPEASR
                     HVHRTIQLIKSHGCQAGLVLNPGTPTEVLDYVLDELDLVLLMSVNPGFGGQAFIPSTL
                     DKLRKVRAMIDATGKPIRLEVDGGVKADNIGAIAAAGADAFVAGSAIFREPRTREAYA
                     DSIARMRGAVSTAR"
     gene            9970..10926
                     /gene="yegS"
                     /locus_tag="PK399_07780"
     CDS             9970..10926
                     /gene="yegS"
                     /locus_tag="PK399_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HPW13056.1"
                     /translation="MTTSMTPRWRLILNGKSAGDDDVRDAVTALRDAGVQLDVRVTWE
                     AGDAERYVAEAIADGIDAIVAGGGDGTLSEVATTLAHRDESAEALPALGLLPLGTAND
                     FARAAGIPDTPHAALRLLLQQPATPIDLLKLDANGTTHWAANLASGGFGTQVTTETHE
                     GLKKWLGGLAYVVTGLSKLGRIEPLQARLHGPGFDWEGDFIALGIGNGRQAGGGQVLC
                     PDAWIDDGLLDVTIVPPLEGDLLATVGTAMAEGKDAALDRVAIRCALPWVDVTATEPL
                     TLNLDGEPVEATRFRIECMSGRLRMHLPADCPLRGNAAKVAA"
     gene            11017..12522
                     /gene="trpE"
                     /locus_tag="PK399_07785"
     CDS             11017..12522
                     /gene="trpE"
                     /locus_tag="PK399_07785"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008575582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HPW13057.1"
                     /translation="MITHAQFQQFAADAHTPGACNLVPVVREVLSDLDTPLSVYLKLA
                     DGPHTYLFESVEGGERFGRHSIIGLPARRVYEFRGHTLEVREYGEVVETREVEDPLAE
                     VELLRAAHKVPKLDGLPGFAGGLVGWFGFECIEYIEPRLRANAKPDELGTPDILLMLS
                     DELAVFDNLKGRLYLIVHADPREPQAWARANRRLDALSHRLRHGGAPYPETLVPAAID
                     ESDFRSSFTREEYHAVVRKAQEYIRAGDIFQVVPSQRLSVPFTARPVDVYRALRALNP
                     SPYMYFLDTGSTQVVGSSPEILARLQHDADGKGTVTVRPIAGTRPRGKTVEEDQALET
                     ELLADPKERAEHLMLIDLGRNDVGRISEAGSVQVGEQFVIERYSHVMHIVSEVTGTLK
                     PGLSYADVLRATFPAGTVSGAPKIRALEVIRELEPVKRNVYSGAVGYIGWHGDADTAI
                     AIRTAVIQDGRLHVQAGGGVVYDSDPDAEWLETMNKGRALFRAVAEAARGL"
BASE COUNT         1931 a         4276 c         4298 g         2020 t
ORIGIN      
        1 gcgagatccg ctacttcgac atcaagggcg ccaagaccgg cctgctgtcg aaggcaatga
       61 ccgcgccgga cggcatggtc cgcatcccgc tcaacgagtc cagcgacccg aagtcgcaga
      121 tcaacgaata cctggatgtg tacggcggcg aaggcatcca gcacatcgcc tgcttcaccg
      181 acgacatcta cgggaccgtg gagaagatgc gcgagaccgg catcgaattc ctggacacgc
      241 cggacacgta tttcgacgtg atcgaccaac gcatcccggc gcatggcgaa gacgtcgaac
      301 gcctgcgcgc caacaagatc ctgatcgacg ccgatcccga gaccaagcag cgcaagctgc
      361 tgcagatctt caccctcaac gcgctgggcc cgatcttctt cgagatcatc cagcgcaagg
      421 gcaacgaagg cttcggcgaa ggcaacttcc aggcgctgtt cgagagcatc gagcgcgacc
      481 agatgaagcg cggcgtgctg tgatctgttc gagcccctgt cccgcgtgcg ggagaggggt
      541 tgggtgaggg caaccggggt gcggcaggca tgaccatcga aagcaacggc taccagtccg
      601 gcttcggcaa cgagtgcgcc agcgaggcga tcgccggcac gttgccgcag gggcgcaact
      661 cgccgcaggt ggtcgcgcac gggctgtatg cggaacaggt gagcggcacc gcgttcaccg
      721 ccccgcgcca ccagaaccgc cgcagctggc tctaccggat ccgcccggcg gcgatgcacg
      781 ggacgttcgc gttgctcgaa cacgacggct tccacaacga tttcgatacc ggcccggtca
      841 cgcccgacca gttgcgctgg aacccgctgc cgctacctga tgcaccgacc gatttcatcg
      901 atggcctgct caccgtggcc ggcaatggcg ccgcatctgc gggcgtcggc atcggcatcc
      961 acctgtacgc ggccaaccgc gacatgcagg gccgcttctt ctacgacgcc gatggcgagc
     1021 tgctgatcgt gccgcagcag ggccggctgc acatcgaaac cgaactgggc gtgctcgacg
     1081 tggagccgca ggagatcgcg gtgatcccgc gcggcatccg cttccgggtg gcactgccgg
     1141 atggcgaggc acgcggctat gtgtgcgaga acttcggtgc gttcatgcgc cttccggacc
     1201 tggggccgat cggcagcaac ggcctggcca atccgcgcga tttcctcacc ccgaatgcgg
     1261 cgttcgagga cctcgaaggc agcttcgagc tgatcgcgaa attccagggc cacctgtggc
     1321 gcgccgacat cgggcattcg ccgctggacg tggtcggctg gcatggcaac cacgcgccgt
     1381 acaagtacga cctgcgcacg ttcaacacga tcggctcgat cagctacgac catccggatc
     1441 cgtcgatctt cctggtgctg acctcgccca gcgattcgcc cggcgtgggc aacatggatt
     1501 tcgtgatctt cccgccgcgc gtgctggtgg cgcagggcac gttccgcccg ccgtggttcc
     1561 accgcaacat cgccagcgag ttcatgggcc tggtgcacgg tgtgtacgac gccaaggcgg
     1621 acggcttcgc acccggtggg tgctcgctgc acaactgcat gaccgggcat gggccggatg
     1681 ccgcgacctt cgacaaggcc agcgccgcgg acacgtcgaa accggactat gtcgacggca
     1741 ccatggcctt catgttcgag tcgcgcagcg tcatccggcc gacccggcag gcgctggacg
     1801 cggcgcatcg ccagcgcgat taccaggcgt gctgggccgg cttgcgcaag cacttcgtcg
     1861 cgccgcaagg ctgagcatcg aggcgaccgc attcgcgttg ccttgagcgc ggatggcaga
     1921 ctgcgggccc atgaaccgga gcccgccgat gcgcacactg ctttcgatcg ccttgctggc
     1981 cgcccttgcc ggttgcgggc agggcggcac gcccgatgcc acgcccaccg cgcccgtcga
     2041 tgcggcgccg gtcgcggaca cgccgccaac ggcaatcgcc acggacacgc cagcatccac
     2101 cgacgccagc gccggtgggt cgctgcgcgt ggacaggccg gccgatggca cgatcacctt
     2161 caagggcttc ggtccggcca atttcggtgc gacccaggaa gacgtgcgca tggcctgggg
     2221 cggtgacctc ggggatggca aacccgacga ggcgggtggc tgctactacc tggtcccgca
     2281 gccggtgggc accgacggtt tccgcaccgc cttcatgatc gaaggcgaca agttcgtgcg
     2341 catcgacgtg cgccgcgacg acgtggcggc gccggggggc ggcaaggtcg gcatgcgcaa
     2401 ggcgcaggtc gcggccctgt atgccggcat cgaggaaggg ccgcacaagt acaccgatgg
     2461 ccagtacctg cgggtcaagg atccggccgg cggcagcggc gtgatggtgt tcgagaccga
     2521 cggcgcgggc gatgatgcca cggtcaccga gtggcgcatc ggcattccgc cgcaggtgga
     2581 ttacgtcgag ggttgcagct gatcgcgcgg gcatgacgca aggcgggttg cctatactgg
     2641 aaccatggac acgcaagctc ccctgcccaa gcgcgcattg cgcgcgctga accatttcct
     2701 gcaatgggaa tcctccggcg gcatcctgct gatcgtcgcc gcgctggtgg cgttggcact
     2761 cgcgaactcg ccgctggcag gcgcctacgg cgagttccgc gagctgccgg tcgcggtgca
     2821 gttcggcgcg ctgaagatcg ccaagccgct actgctctgg atcaacgacg gcctgatggc
     2881 gatcttcttc ctgctggtcg cgctggagct gaagcgggaa gcgttgtccg gacagttgtc
     2941 gtcgttctcg cagttgtcgc tgccggtggt ctgcgcactg gccggcgtgg ccgtgccggc
     3001 gggcatctat gcctggctca accgtggcga cgcggtggcg atgcaggggt gggccgttcc
     3061 cgccgccacc gacatcgcgt tcgcgctggg gatcctgtcg ttgctcgggt cgcgcgtccc
     3121 ggtggcgatg aagctgttgt tgtcgacgat cgcggtgatc gacgacctgg cggcgatcgt
     3181 catcatcgcg ttgttctata ccagcgacct gtcggtggtc gcgctgggct gggcgggtgc
     3241 ggcgatcggc ttgatggtgg tgctcaatcg ccgcggcgtg caggcgctca cgccctacct
     3301 gctgctcggc acgatcgtgt gggtggccgt gctgaagtcg ggcgtgcacg ccacccttgc
     3361 cggtgtcgcg acgggcctgc tgattcccca cgtgaaccgc aaggatgcga tcgacgacga
     3421 atcccagcat tcgccgctgg aacacctgga gcacgcgctg catccctggg ttgcctacct
     3481 catcctgccg gtgttcgcgt tcgccaacgc gggcctggcg ctgggcggct tcgcgctgga
     3541 cgacctgctg accccggtgc cgctcggcgt ggcgctgggc ctggtcgcgg gcaagccggt
     3601 tggcgtggtg ttggctgccc tgctggcgcg agccaccggg ttttcgcgct tccccgaggg
     3661 catggactgg aaggcgatgg tcgggctggg gctgctgtgc gggatcggtt tcaccatgag
     3721 cctgttcatc gcctcgctcg cattcggcga tgacgccggg ctgttcccca gcgcggtgct
     3781 gggggtgttg tgcgcgtcgc tggtttccgc gcttgccggg cttgcctggc tgtgggcgac
     3841 cttgccgcgc gcgcctgcgg ccgcaggcca tccaccacgc gccgactaga ctgcccggat
     3901 gatccgtcgc gcacctcgct tcccgcgtct cgccctgttg ccgatggcag ccctgcccac
     3961 caccgcatgg gcgatggcgc ctgcgcagga cttcaccgcg cactggtccg gcctggtcgc
     4021 ggtcgggatt ttcgtcgccg cgtacgtgct ggtgatcgcc gaggaatcca ccggcctgcg
     4081 caagtcgcag ccggtcatgc tggccgccgg tgcgatctgg gcggtgctgg ccatcgcctc
     4141 cgcgcgggcc ggattgtccg acgccgtcca cgaggcggtc cgcgactacc tgctcgagtt
     4201 cgccgagctg ctgctgttcc tgctggcggc gatgacctac gtgaacgcga tgagcgagcg
     4261 cgggttgttc gaagtgctgc gggtctggct gctgcgcagg gggctgggtt accgcgcgct
     4321 gttctggacc accggcatgc tggcgttctg catgtcgccg ctgatcgaca acctcaccac
     4381 ggcgctggtg ctgtgcgcgg tggtgctcgc ggtggggcgc gacagcccga agttcgtcgc
     4441 ccttgcctgc atcaacatcg tggtggcggc caatgccggc ggcgcgttca ccccgttcgg
     4501 cgacatcacc acgctgatgg tctggcaggc cgggaaagtg ccgttcgcct cgttcttcgc
     4561 gctgttcctg ccggcgctgg tgaactgggg cgtgccggcg ttgtgcatgc atgccgcggt
     4621 gcctgggggc accccggtcc tcaccgacgc acaggcacgc ctgcgtcgcg gcgggctggt
     4681 gatcgtcggc ctgttcggcc tgaccatcgc gatggcggtc ggcttccacc agttcctgca
     4741 cctgccgccg ttcctcggga tgatgaccgg gctggcgctg ctcaagttgt acggctggca
     4801 tctcacccgc gcggccaccg cgcagcaggc ggccgagttc gcccgcgagg gcgcccccgg
     4861 cgacgtggat gccttcgaca gctacgaaca ggtggcgcgc gcggagtggg acacgctgtt
     4921 gttcttcttc ggcgtcatca tgtgcgtggg tgcgttgggg tactgcggct acctggcgct
     4981 gctgtcgcag cagttctatg gcggcctggg gccgaccacc gccaacgtcc tggtcggctt
     5041 gctgtcggcg gtgctggaca acatcccgat gatggtcgcg gtgctgcaga tggcacccgc
     5101 catggacacc ggccagtggc tgctggtgac cctgaccgcc ggcgttggcg gcagcctgtt
     5161 gtcgatcggg tccgccgccg gggtggcgct gatggggcag gcgcatggcc agtacacgtt
     5221 cttcagccac ctcaagtgga cctgggcgat cgccctgggt tacgccgcca gtgtcggcgt
     5281 gcacctgtgg ctcaacgccc gttatttcac cggcgtggcg tgatgcgcct ggatgacctg
     5341 gtgcggatgg caccggcgcg atgaagacgg cgttggtgtt cggtgccagc gggcaggttg
     5401 gcctgccgtt gctggatcgc ctgggcgatg ccggctggcg gatttacgcg gtctcgcggc
     5461 aggcgcgctc cgacactccg ggtcggcact ggctgcaggg cgatttcgcg cgcatgcccg
     5521 ggttgccgcg cgacgtcgac gcactcttca gctgcggccc gctggacctg ttcgcgcgct
     5581 ggtatgccac cgcggacatc gccgtgccgc gggtggtggc gttcggttcg accagcgcca
     5641 ctagcaagca tgcctccggg gacgatggcg aacgcgacgt cgcccgtcgc cttttgggtg
     5701 cggaagcggc ggtgttcgag gccagcgagc aacgcggcgc cggcgcgacg atgctgcgcc
     5761 cgaccctggt ctatggcgcc ggccgcgatg ccaccctgac ccgcatcgtc ggcatggcaa
     5821 ggcgctgggg ccgcttcgtg ctgccgcgtc gtgccgatgg cctgcgccag ccggtgcatg
     5881 tgcaggacct ggccgatgcc gccttcggcg cgagccttgc gcctgcgacc cctggtcgca
     5941 gctacgacct gcccggcggc gagaccctgg cctatcggga gatggtgcgg cgggtgctgg
     6001 cctgcctgga tccgccgctg ccgttgctgg aattgccgat gccgctgttc cgcgcggcgc
     6061 tggcggcagc tcaggcgcgc ggcatcgcgc gcgacctcac gcctgccgcc gtcgcgcgca
     6121 tgcgcgaaga cctggccttc gatgccgcgc cggcgcgccg cgacttcggc tattcgccac
     6181 gcgggttccg ccccgaggcg cgcatgttcg acgcgaccga ccgccgcgac tgagcgctac
     6241 aactcctcgg caggtggcaa ggggcgctgg cgcacgccgg cgcgcaaggc gaccaccagc
     6301 acgccgccca gcaccatcgc cccgccgatc cacagggcaa gccctgggcg gtcgccccag
     6361 aacaggatcc ccagcagcac cgccaggact ggcgtgagca gcaggtaggg cgtgaccgtc
     6421 gccaccggat gccgttgcac cagccagtag aagacgccgt ggccgagcag cgacgagaac
     6481 accgcggcgt agaccgccgc gccccacgcg atccagctgg catcccgcag cgcggcgaag
     6541 ctgccgggtt cgaacagcgc gctgatcgcc agcagtggca gcaggctgag caccgcggtc
     6601 cagccctgct ggtcgaccat gcgcacgtcc ttcagcccgc gcatcagcac cgtgccgatc
     6661 gccaccatca gcgacgagcc catcatcacg aacagcgccg ccggctgcgc cagcaccatc
     6721 gggtcgaagc cgagcaccag caccccggcg aagctgatcg ccacgcccac gccggtgcgc
     6781 cagccgaagc gctcgcccag ccaccaccac gccagcagca ccgacatcgg cacgtagacc
     6841 tgggtgacga tcgccggcga cgacaggttg cccgccagct tcagcgccag gaagctggtg
     6901 ccgaagtgca ggacgccgtt gagcatcgcc accgcgaaca gccgcttcca ttgcgcgcgc
     6961 ggcggcaccc gcatgaaccc gaccagcagg acggccagca ggcccatccg catcgcggtg
     7021 aacaggaacg gagggacctc gcgcagggcg tgcgcggagg tcaggaaatt ggtcgcccag
     7081 aacaggcaga tgagcaggac caggacgagg tcgcgcccgc gcaaccccgt cgcggtcatg
     7141 gtgcggtcac cgcatgatca ccagcggatg ccgagcttgc ggtacgcctt ggccagcacc
     7201 caggcggggc cgatcagcag gtacttgagg tcggtgaaga aactcggctt cttgccttcg
     7261 tacaacttgc tgtggccgat gaactgcgcg atccacgcga ccacgaacac ccccagcgcg
     7321 agcttgaaca ggcccggcgt gcccagcgtg tcgtgcagcc agcgggtcag ccacgccatc
     7381 gccacgaaca ccgccagcat gccgaagccg atcgcccgcg acgcgcggta atagaacatc
     7441 cagccgccga acatcgccag cgcggcccac aggccggcgc ggaacagcgt gcccggcacc
     7501 gggatgcacc acagcaggcc gatgacgctc cacaggatca gcggcaccgc gaacacatgg
     7561 atgcgctggt tggtcgggtt gacgtgatcg cccgagtaat gcgtgaacca atggtcgatc
     7621 gggcggttgc cggcctcgtg ttgcaccgtg gcgttcatgc gttctcccgt ttgcgcgtgc
     7681 gcctggcgag gatactccag cgcacggtcg cgggctcagt cgacggaaat cccggccagc
     7741 cgatccagcg cctcggcgta cttggcgcgg gtcctggcga tgacctccgc gggaaggcgc
     7801 gggccgggcg gggtcttgtc ccagtccagc gtttccaggt aatcgcggac gaactgcttg
     7861 tcgtagctcg gggggctggt gcccacgcga tagtcgtcgg cgggccagta gcggctggaa
     7921 tccggggtca gcacctcgtc catcacgtag agcttgccgt cggcatcggt gccgaactcg
     7981 aacttggtgt cggccaggat gatcccgcgc gttgccgcgt agtcgcgggc gaaggcatac
     8041 aacgccaggg tcgcatcgcg cacctgctcg gcgcgctcgc ggccgaccag gtccaccgcg
     8101 gtggcgaagt cgatgttctc gtcgtggtcg cccaccgcgg ccttggtcga cggggtgaag
     8161 atcggcgtgt cgagttgctg cgcctgctgc aggcccggtg gcaggtcgat gccgctgatc
     8221 gcgccgctgc gctggtagtc cttccagccg ctgccgatca cgtaaccgcg ggcaatgcat
     8281 tccaccggca ccggggtcag cttcttggtc accaccgcgc gcttcgcgta cagcgccgga
     8341 tcgacgccgg ccggcagcac cgatgcgaca tcgatcccgg tcaggtggtt ggcgatgatg
     8401 tgcctggtct tgtcgaacca gaaattgctg atctggcaga gcatctcgcc cttgcccggg
     8461 atcgggtcgg gcaggaccac gtcgaacgcg ctgaggcggt ctgtcgcgac catcagcagg
     8521 gtgtcgttgc ccaggtcgaa cacgtcgcgg accttgccgc ggtggcgcag ggtgaggccg
     8581 ggcaaatcgg actgcagcag ggtggtggcc atgcgagggc ggcgggcatc gtggggcgca
     8641 cagtgtagcg gctgcccccg ccggctccgc agggacggcc acccgtgggc gccgcccctc
     8701 cgtggcagcc ccatgcaggc tcgcctcgca gccgccttgt aagatggcgc gcatgaaacg
     8761 ctggtacggc aaattcttcg ggttgatcgc aggctggctg ctgttccggc atccggccgg
     8821 cgggctgctg ggcctgctca tcgggcatgc cttcgatgcc gactggttca agtccgcgcg
     8881 cgacaacccg tacggcgtgc tgcaggtctc gagcgacgcc agcgacgccg aggtcgaacg
     8941 cgcgtatcgc cggctgatct cgcaatacca cccggaccgc ttggccggtg ccgccccgca
     9001 actgcaacgc caggccgaag aaaaggcccg cgagatcaac accgcctacg accgcatcaa
     9061 gaccctacgg aagacgtgag catgtcgcct gccccacccc tggactgcgt gatcgcaccg
     9121 tccatcctct ccgccaactt cgcgaagctg ggcgaagagg tcgacaacgt gctggccgcc
     9181 ggtgcggact gggtgcactt cgacgtgatg gacaaccatt acgtgcccaa cctcaccatc
     9241 ggcccgctgg tctgcgaggc gctgcgcgcg catggcgtga ccgcgccgat cgacgtgcac
     9301 ctcatggtcg agccggtgga ccgcatcgtc cccgacttcg ccaaggccgg cgcctcgatc
     9361 atcagcttcc atccggaggc cagccgacac gtgcatcgca ccatccagct gatcaagtcg
     9421 cacggatgcc aggccgggct ggtgctgaat ccgggcacgc cgaccgaagt gctggactac
     9481 gtgctggacg agctcgacct tgtgctgttg atgtcggtga atcctggctt cggcgggcag
     9541 gcgttcattc cctcgacgct cgacaaattg cgcaaggtgc gggcgatgat cgacgcgacc
     9601 ggcaagccga tccgcctgga agtcgatggc ggggtgaagg cggacaacat cggtgcgatc
     9661 gccgcggcag gcgcggatgc gttcgtcgcc ggcagcgcga tcttccgcga gccgcgtacc
     9721 cgcgaggcct atgcggacag catcgcccgc atgcgcggag ccgtctcgac cgcgcgctga
     9781 cccgagcggg atccgggcag cgcgcaaaaa aaaaccgatg ccgccatggg gaggggagcg
     9841 gcaccggtct ggaagagtgg ggcgtcctgc ccatccgtca tccctgcgat ggactcctat
     9901 cctccgcatc gcacgtgacg cccgtgtgac atccgcatgg tcttaacgtg tccgggctag
     9961 cctgtgcgca tgaccacctc catgacaccg cgctggcgat tgatcctcaa tggaaaatcc
    10021 gccggcgatg acgatgtgcg cgatgccgtg actgcgcttc gcgatgcggg cgttcaactc
    10081 gacgtgcggg tcacctggga agcgggcgac gccgaacgct acgtggccga ggcgatcgcc
    10141 gacggcatcg acgccatcgt cgccggtggc ggcgacggca ccttgagcga agtcgcgacc
    10201 accctggcgc atcgcgatga atccgccgag gcattgcccg cgctcggcct gttgccgctg
    10261 ggcaccgcca acgacttcgc ccgcgcggcc ggcatcccgg acacgcctca tgccgccctg
    10321 cgcctgctcc tgcagcaacc ggcgacgccc atcgacctgc tcaagctgga cgccaacggc
    10381 acgacccatt gggcggcgaa cctggccagt ggcggcttcg gcacccaggt caccaccgag
    10441 acccacgaag gcctgaagaa atggctgggc gggctggcct acgtggtgac cggactctcg
    10501 aagctggggc gcatcgaacc gctgcaggcg cggctgcatg gccccggctt cgattgggag
    10561 ggcgacttca tcgcgctggg catcggcaat ggccgccagg ccggcggcgg gcaagtgctt
    10621 tgccccgacg catggatcga cgacggcttg ctcgacgtga ccatcgtgcc gccgctggaa
    10681 ggcgacctgc tggcgaccgt cggcaccgcg atggccgagg gcaaggatgc cgcgctggat
    10741 cgggtcgcga ttcgctgtgc gttgccgtgg gtagacgtca ccgcgaccga gccgctgacc
    10801 ctcaacctcg atggcgagcc ggtggaggcc acccgtttcc gcatcgagtg catgtccggg
    10861 cgcctgcgca tgcacctgcc cgcggattgc ccgctgcgcg ggaatgcggc taaagttgcc
    10921 gcatgaaccg ggcgaccccg tctttcgagc atggttggcg atggtggcga gattccgccg
    10981 ccccgatcgc ccgtatgccc ctgaaggacg ccccgcttga tcacgcacgc ccaattccag
    11041 cagttcgctg ctgacgccca cactcccggc gcctgcaacc tggttcccgt cgtgcgcgag
    11101 gtcctgtccg acctcgacac gccgctgtcg gtgtacctga agctggccga cggcccgcat
    11161 acctacctgt tcgagtcggt cgagggcggc gagcgcttcg gccgccattc gatcatcggc
    11221 ctgccggcgc gacgcgtgta cgaattccgc ggccacacgc tggaagtccg cgaatacggc
    11281 gaggtcgtcg aaacccgcga ggtcgaggat ccgctggccg aagtcgaatt gctgcgagcc
    11341 gcgcacaagg taccgaaact cgatggcttg cccggctttg cgggtgggtt ggtcggctgg
    11401 ttcggcttcg aatgcatcga gtacatcgag ccgcgcctgc gcgccaacgc gaagccggac
    11461 gaactcggca cgcccgacat cctgctgatg ctcagcgacg agctggcggt gttcgataac
    11521 ctcaagggcc ggctgtacct gatcgtgcac gccgacccgc gcgagccgca ggcctgggcg
    11581 cgcgccaacc gccgtctcga tgcgctcagc caccggcttc gccacggcgg cgcgccctat
    11641 ccggaaaccc tggtgccggc ggcgatcgac gagtccgact tccgctccag cttcacccgc
    11701 gaggagtacc acgccgtggt gcgcaaggcg caggaataca tccgcgccgg cgacatcttc
    11761 caggtggttc caagccagcg cctgagcgtg ccgttcaccg cgcgtcccgt cgatgtctat
    11821 cgcgcgctgc gtgcgctgaa tccgtcgccg tacatgtact tcctcgatac cggatcgacc
    11881 caggtcgtcg gttcctcgcc ggaaatcctg gcgcgcctgc agcacgatgc ggacggcaag
    11941 ggtacggtca ccgtgcgccc gatcgccgga acgcgtccgc gcggcaagac cgtcgaggaa
    12001 gaccaggcgc tggagaccga attgctcgcc gatccgaagg aacgcgccga gcacctgatg
    12061 ctgatcgacc tgggtcgcaa cgacgtcggc cgcatcagcg aggccggcag cgtgcaggtc
    12121 ggcgagcagt tcgtgatcga acgctacagc cacgtcatgc acatcgtcag cgaagtcacc
    12181 ggcaccctga agccgggcct gagctatgcc gacgtgctgc gcgccacgtt ccccgccggc
    12241 accgtcagcg gcgcgccgaa gatccgcgcg ctggaagtca tccgcgagct ggaaccggtc
    12301 aagcgcaacg tctattcggg cgcggtcggt tacatcggct ggcacggcga cgccgacacc
    12361 gccatcgcca tccgcaccgc ggtgatccag gacggtcgcc tgcacgtgca ggccggcggc
    12421 ggcgtggtct acgactccga tccggatgcg gagtggctgg agacgatgaa caaggggcgc
    12481 gcgctgtttc gcgcggtggc cgaagcggcg agggggttgt gatgg
//
LOCUS       DAOOCX010000069        12122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_4900_length_12122_cov_8.520759, whole genome shotgun sequence.
ACCESSION   DAOOCX010000069 DAOOCX010000000
VERSION     DAOOCX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12122
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4900_length_12122_cov_8.520759"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PK399_07790"
     CDS             complement(<1..212)
                     /locus_tag="PK399_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPW13058.1"
                     /translation="MPAYKIQEHSFDMIVVGAGGAGLRATFGLAQKGLRTACISKVFP
                     TRSHTVAAQGGISAALGNMGEDDWRF"
     gene            complement(217..612)
                     /gene="sdhD"
                     /locus_tag="PK399_07795"
     CDS             complement(217..612)
                     /gene="sdhD"
                     /locus_tag="PK399_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HPW13059.1"
                     /translation="MSTHGKDLRTPLKRAIGLGSAKDGTGHFMLQRITAIALLLAGAW
                     LIGLLLSLNSTDYVFVRATVADPLNATVLVAFLIAACWHAKLGLQVVIEDYVHAPLLA
                     GAAHLANIFACALLAIAGVFAVLRIALGA"
     gene            complement(609..1019)
                     /gene="sdhC"
                     /locus_tag="PK399_07800"
     CDS             complement(609..1019)
                     /gene="sdhC"
                     /locus_tag="PK399_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HPW13060.1"
                     /translation="MAAPDPNVRTRPLSPHLDVYKWQVQMLTSILHRATGIALVAGML
                     GLVWGLVALAGGEKSWATFATCATSLLGKLVLLGFSWALAYHLINGIRHLVQDAGHGY
                     AIPDFVRSSWISIIGSVVLVVVVWAIVLLRGGAA"
     gene            complement(1067..1267)
                     /locus_tag="PK399_07805"
     CDS             complement(1067..1267)
                     /locus_tag="PK399_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HPW13061.1"
                     /translation="MNASIIGQSLSSPEPVEAAVPSPEPATPTASKPEPVIDPAVEIG
                     GREGPEPTRYGDWEKNGRCIDF"
     gene            1295..2173
                     /locus_tag="PK399_07810"
     CDS             1295..2173
                     /locus_tag="PK399_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HPW13062.1"
                     /translation="MSHNPQAASAQDFELPGHDVLAITGRDAVAFAQAQFMNDIAALG
                     DGQWHWNGWLTAKGRVIALFALLRIDAETLWLVLPDVDATALATQLQRFVFRSKLKLE
                     PLADLHAVSAFAPPAAASGAMSAVVDGGIELDMGGDGGTRRLRIATTNAASDKALVSR
                     WRAFDLAHGLPRLDADQAETWTPQQLSLERLRAFSVKKGCYPGQEIVARTHFLGKVKR
                     GLVRLQGAGVPELAEVMDGEPVGPAAQTARPIGQLICAEADEGLAVMPLEAGDGPFAT
                     GNLPCTRLSLLDGLAR"
     gene            2215..3060
                     /gene="fdhD"
                     /locus_tag="PK399_07815"
     CDS             2215..3060
                     /gene="fdhD"
                     /locus_tag="PK399_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPW13063.1"
                     /translation="MNARIWHPEPTEGAAARTVQRRDGKGAHNVRDEVACEVPVALHY
                     NDQPFAVLMATPCDLADLAVGFSLSEALIPDAGELQIEAIDESIDGIAIRMRIPEPRH
                     AALADRQRALPGYGSCGICGSTSIEAVLRAPPRIASTPRIAETALHRALDALHECQPL
                     NAATGATHAAGFADRSGTLLLVREDVGRHNALDKLVGAMATQGIDAGSGFAIVTSRAS
                     YELAMKAAHVGIPLLAAISAPTTLAIALAESADLCLVGFARDGHCAVYAHPERLIPSS
                     AEPSR"
     gene            3057..5441
                     /locus_tag="PK399_07820"
     CDS             3057..5441
                     /locus_tag="PK399_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005915320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HPW13064.1"
                     /translation="MTEPPKIAPYDGPAGGWPALKSVSEHLLQQDIPVLGAKTLLSAN
                     QPDGFDCPGCAWPDREHTSTFEFCENGAKAVAFEATTRRVGPGFFAQYSVADLARYSD
                     HWLEDQGRLTHPLRYNPKTDRYEAVSWDTAFRFIASKLNALASPDEAIFYTSGRTSNE
                     AAFLYQLFVRQFGTNNFPDCSNMCHEPSGVALGQQIGVGRGTVSLQDFEEADLILVFG
                     QNPGTNHPRMLGELRRAVKRGTSVLAFNPLRERGLERFADPQDALEMLHGGSTKIASD
                     YYQLRIGGDLAAVTGIIKHVLERGARAHAEGKPALLDEGFIETHTEGFDALLASVRAA
                     HWRQIEAESGLSEERLRDAGERFLRSERAIVCWGMGITQHKRAVATIQMLVNLMLLRG
                     HIGRPGAGICPVRGHSNVQGDRTMGIHEKPAPALLDRLGEVFGFEPPREHGVDVVGAI
                     EAMRDGRGKVFFAMGGNFATATPDTEATHAALRNCELTVHVATKLNRSHLVHGKDALI
                     LPCLGRTEIDLQAGGPQAVTVEDSMSMVHLSAGINAPASPELLSEPAIVARLAQATLG
                     KRSDTPWRWLVEDYDRIRDQIARVFEDFHDFNQRVRVPGGFRLTNTAALRQWKTATGK
                     ANFFAHRLAEDDPLPDIPGAQLFTLTTVRSHDQYNTTIYGLQDRYRGVHGERRVLFAH
                     ADDIAGLGFAAGQRVDIESVHDDGVQRIVRGFLLAAYDIPRGCLAAYYPETNPLVPLD
                     SFAIEARTPTSKAIPVILHAHDAAAAHAQPRDIAATLVGKKREKPALSARKPPQ"
     gene            complement(5449..7725)
                     /locus_tag="PK399_07825"
     CDS             complement(5449..7725)
                     /locus_tag="PK399_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW13065.1"
                     /translation="MFATIPNPIPARMKGVNRAEVCDVNFSEFVKSWDGIPDAKPAPD
                     EAILEGSALDARGFRDLFESQLISRHLDLMARVLRVQNKVFYTIGSSGHEGNAMVARA
                     ARHTDPAFLHYRSGGFMAERFRKLPGMDPIMDSALSFAASAEDPASGGRHKVWGSKPL
                     WVLPQTSTIASHLPKALGTAVAIEAARRIGHQLPIPDDSIAICSFGDASANHATAQTA
                     FNAASWTAYQKLPAPVLYVCEDNGIGISVKTPTGWIGESFRNRPDLDYFFADGLDLAN
                     GYADVQRAVEHCRRTRRPTFLHLRTTRIMGHAGTDFEIEWRSIEELCAVEAGDPLLRS
                     AQIALESGLMSKDEILALYEDTRRKCFAAAEDADQRARIETLEHVMRPLAPYTPDKVL
                     AEATRVPSQEARIKAFGSEEKLPEKQVPRHLAIQINQALHDLFAKYPETLLFGEDVAQ
                     KGGVYTVTKGLHKAFGNKRVFNTLLDETMILGMAQGFANMGMLPIPEIQYLAYLHNAI
                     DQIRGEAASLQFFSNDQFRNPMVVRIAGLGYQRGFGGHFHNDNSMTALRDIPGLVVGC
                     ASRGDDAAMMLRTLTALAKVDGRVSVFLEPIALYMTKDLYEPGDGQWLTEYPAPDQAL
                     VLGEERVYNADATDCVIFSYGNGMPMSLRAARQIEAKHGWKVRVVDLRWLVPLNHKAI
                     ARHARECKRILVVDEGRFSAGIGEGVITAITEAGLGGKPGRRVCGADTYTPLAGAAFL
                     VIPKDEDIVAAADVLAAG"
     gene            complement(7776..8459)
                     /locus_tag="PK399_07830"
     CDS             complement(7776..8459)
                     /locus_tag="PK399_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HPW13066.1"
                     /translation="MGGASQGERGVITVHGFSPSGNCHKVKLLLEQLGGRLDRDYRWV
                     ETDSSRGATRTPDYLAKNPNGKVPMIELDDGRVLVESNAILCWLADGTSFMPADPWQK
                     AQALSWLFFEQYSHEPCIAVARFICGWTPADSPRRADLPKLRERGHAALAVMERHLAT
                     KAWFTGDGYGIADIALYAYTCVAPHGGIALDAYPALRGWLSCVEATPGFVRLPAPDAA
                     AQALIDQST"
     gene            complement(8426..8980)
                     /locus_tag="PK399_07835"
     CDS             complement(8426..8980)
                     /locus_tag="PK399_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW13067.1"
                     /translation="MSLSDIRIETERLVLRQQRIEDFERFAELRADEEASRHIGGVMT
                     RAAAWRRFLEMPGAWAVQGFAMFSVVEKASGNWIGNIGPWKPEGWPGTEVGWALHRDA
                     WGKGYALEAATAAIDWAFAELGWTEVIHSIDLDNTASQALAQRLGSRNRGRGSLPPPF
                     QDVVIDIWGQTREEWVARRKGSVA"
     gene            complement(9084..10247)
                     /locus_tag="PK399_07840"
     CDS             complement(9084..10247)
                     /locus_tag="PK399_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPW13068.1"
                     /translation="MALPPYDLFDVRSLLTEEERAVQDAVARFTDERVIPIIGDAFDQ
                     GRFPKELVAEIAEMGLLGSSLPEKYGCAGLNAVSYGLICQELERGDSGIRSFVSVQSS
                     LCMYPIYAYGTEEQRMRWLPQMAAGKVIGCFGLTEPQGGSDPANMKTRAVRDGDDWIL
                     NGSKMWITNGNLADIAIVWAQTDDGIQGFLVEKGFAGFTAQEIHKKMSLRASVTSALF
                     FDNVRVPEANRLPNVKGLKGPLGCLTQARYGITWGPIGAAIACLDEALKYTKERILFD
                     RPVAATQSAQIKMADMARRITLAQMLVLQLGRLKDAGTMQPAQVSLAKWNNCRMAIDI
                     ARECRDLLGGAGITTEHAAIRHALNLESVITYEGTETVHQLVIGRELTGINAF"
     gene            complement(10461..11240)
                     /locus_tag="PK399_07845"
     CDS             complement(10461..11240)
                     /locus_tag="PK399_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017161798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HPW13069.1"
                     /translation="MKGNTLAIALGALLVGGVATAGFINNNNARGSTNPMAVDSAYRG
                     TDDGAVGLAYADVVSVEPVIEREERYATVIGADPVRESSSTTTPQQVCADVAVQERMP
                     ERDGNVGGTVAGALIGGLVGNQVGGGNGRKAATAAGAVAGGFIGNRVDRNHVGGQVVS
                     RVDRRCRTVNNTSTSSRTIAWNVTYRNADGTTGTLQTERKPGERIALGSDDRTVAYDV
                     RYMLDGDIRTVRMAHPPGQRVPLLDGEVVTQVASTTGEPNG"
     gene            11396..11638
                     /locus_tag="PK399_07850"
     CDS             11396..11638
                     /locus_tag="PK399_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13070.1"
                     /translation="MPNPLLLPLLNWARKLRYPVLFKLTAALFAISVLLPPGIDPIPF
                     LDEIVFGLGTLLLANWKTRKPPAGNEKPPIDGEARR"
     gene            11643..>12122
                     /locus_tag="PK399_07855"
     CDS             11643..>12122
                     /locus_tag="PK399_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPW13071.1"
                     /translation="MLVDSHSHFDAPEFDADRAAAHARALAAGVDIQIVPAVEAATWP
                     KLKAVCAEFSGLHAAYGLHPMYLDAHRPAHLDDLRNWIERERPVAVGECGLDYFVEGL
                     DHEAQQLFFDGQLRLARDFDLPVIVHARRAVDAVIASLRKVGGLRGVVHSFSGSAEQA
                     "
BASE COUNT         1989 a         4143 c         3992 g         1998 t
ORIGIN      
        1 tggaagcgcc agtcgtcctc gcccatgttg ccgagcgcgg cggagatgcc gccctgggcc
       61 gccacggtat ggctgcgggt cgggaagacc ttgctgatgc aggcggtccg caggcccttc
      121 tgggccagcc cgaaggtggc gcgcaggccg gcgccgccgg cgccgaccac gatcatgtcg
      181 aagctgtgtt cctggatctt gtaggcaggc acgatgtcag gctcccagcg cgatgcgcag
      241 caccgcgaac acgccggcga tggccagcag cgcgcaggcg aaaatgttgg cgagatgcgc
      301 ggcgccggcg agcagcggcg cgtgcacgta gtcttcgatg accacctgca ggcccagctt
      361 ggcgtgccag cacgcagcga tgaggaacgc gaccagcacc gtcgcattga gcggatcggc
      421 cacggtcgcg cggacgaaga cgtagtcggt gctgttgagc gagagcagca gcccgatcag
      481 ccacgcaccg gcgagcagca gcgcgatcgc ggtgatccgc tgcagcatga aatgaccggt
      541 gccatccttc gcggaaccca ggccgatcgc gcgcttgagc ggcgtgcgca ggtccttgcc
      601 atgcgtgctc atgcggcacc tccgcgcagc agcacgatcg cccacacgac cacgaccagc
      661 accacgctgc cgatgatcga gatccagctg ctgcggacga aatccgggat cgcgtagccg
      721 tggccggcat cctggaccag gtggcggatg ccgttgatga ggtggtaggc cagcgcccag
      781 ctgaagccga gcaagaccag cttgcccagc agcgaggtcg cgcaggtggc gaatgtcgcc
      841 caggatttct cgccacccgc caaggccacc aggccccaga ccaagcccag catgccggcg
      901 accaaggcga tgccagtggc ccgatggagg atcgaggtga gcatctgtac ttgccacttg
      961 tagacatcca agtggggcga caacggccgc gtgcggacgt tcggatcagg agcagccatg
     1021 ggcgtgcgct ctccgggctg ggcggctcgc gccgcgtggc gatgtctcag aagtcgatgc
     1081 agcggccatt cttttcccag tcgccgtagc gcgtcggctc ggggccttcg cgaccgccga
     1141 tctcgaccgc ggggtcgatg acgggttcgg gcttcgaagc agtcggcgtc gcgggttccg
     1201 ggctcggaac cgcggcctcg acgggctcgg ggctggaaag ggattggcct atgatcgacg
     1261 cattcacaac cccgcgagtt taagtcccgc ccccatgtcc cacaacccgc aagccgcatc
     1321 ggcacaggat ttcgaactgc cgggccacga tgtcctggcc atcaccgggc gcgatgcggt
     1381 ggccttcgcg caggcgcagt tcatgaatga catcgccgca ttgggcgatg gccagtggca
     1441 ctggaacggc tggctgaccg cgaaaggccg cgtgattgcg ctgttcgcgc tgctgcggat
     1501 cgatgccgag accctgtggc tggtcttgcc tgatgtcgat gcaacggcat tggcgacaca
     1561 actgcaacgg ttcgttttcc gcagcaagct gaagctggaa ccgcttgccg acctgcatgc
     1621 ggtgtcggcc ttcgcgccac cggctgcggc atcgggtgcg atgtcggcgg tcgtcgatgg
     1681 cgggatcgag ctcgacatgg gcggcgatgg tggaacgcgt cgcctgcgca tcgccacgac
     1741 caacgcggcg tccgacaagg cgctggtatc gcgctggcgc gccttcgacc tcgcccatgg
     1801 cctgccccgg ctggacgccg accaggccga aacgtggacc ccgcaacagc tttcgctgga
     1861 gcggctgcgc gccttcagcg tgaagaaggg ctgctatccc ggccaggaaa tcgtggcgcg
     1921 cacgcatttc ctcggcaagg tgaaacgcgg gttggtgcgc ctgcaaggcg ccggcgtccc
     1981 tgagctggct gaagtgatgg acggtgagcc tgttggcccc gccgcgcaga ccgcacgtcc
     2041 catcggacag ttgatctgcg ccgaagccga cgaaggcctc gcagtgatgc ccctggaagc
     2101 cggcgacggg cctttcgcca ccggcaacct gccctgcacg cgcctgtcgt tgctcgatgg
     2161 cctcgcccgc tgaagcgtga tgccgtgccg ttggcgcggc gctatcctgc cgaggtgaac
     2221 gcacgtatct ggcatcccga accgaccgaa ggcgccgcag ctcgcacggt gcaacgccgt
     2281 gatggcaaag gcgcgcacaa cgtccgcgac gaagtcgcct gcgaagtccc ggtcgccctg
     2341 cactacaacg accagccgtt cgcggtgctg atggccacgc cttgcgacct ggcggacctt
     2401 gcagtgggct tctccctcag cgaggcactg atccccgatg ccggcgaact gcagatcgag
     2461 gccatcgatg aatcaatcga tggcattgcc atccgcatgc gcatcccgga accgcgccat
     2521 gccgcgctcg ccgaccgcca gcgcgccctg cccggctatg gcagttgcgg gatctgcggc
     2581 agcacgtcga tcgaggcggt gctgcgcgcg ccaccccgca tcgccagcac gccacgcatc
     2641 gcggaaaccg cgctgcatcg cgccctcgac gccctgcatg agtgccaacc gttgaatgcg
     2701 gcaacgggag cgacccacgc cgccggcttc gccgatcgca gcggaacttt gctgctggtg
     2761 cgcgaggacg tgggccgcca caacgcgctc gacaaactgg ttggcgcgat ggcaacgcaa
     2821 ggcatcgatg ccggcagcgg cttcgccatt gtcaccagtc gcgccagcta cgagctcgcg
     2881 atgaaggccg cgcatgtcgg cattccgttg ctggcggcga tctccgcgcc caccacgctg
     2941 gcgatcgcgc tggccgagtc ggccgacctc tgcctggtcg ggttcgcccg cgatggccat
     3001 tgcgccgtgt acgcgcaccc cgagcgactg attccatcgt cagcagagcc gtcgcgatga
     3061 cagagccccc gaagatcgcg ccctacgacg gcccagcggg tggctggccc gcgctcaagt
     3121 cggtaagcga acacctgttg cagcaggaca tccccgtgct gggtgcgaag accctgctct
     3181 cggccaacca gccggatggc ttcgactgcc ccggttgcgc gtggccggat cgcgagcaca
     3241 cctcgacctt cgagttttgc gagaacggcg ccaaggccgt cgccttcgag gccaccacgc
     3301 gccgggtcgg gcccgggttc ttcgcgcagt acagcgtggc ggacctcgcg cgctacagcg
     3361 accactggct ggaagaccag ggccgactca cccatccgct gcgctacaac ccgaagaccg
     3421 atcgctacga agcggtgtcg tgggacacgg ccttcaggtt catcgcgtcg aagctcaacg
     3481 cgctcgcctc gccggacgag gcgatcttct acacctccgg ccgcaccagc aacgaagcgg
     3541 cattcctcta ccagttgttc gtgcgccagt tcggcaccaa caatttcccc gactgctcga
     3601 acatgtgcca cgagccgtcc ggcgtggcgc tgggccagca gatcggcgtc ggcaggggca
     3661 ccgtttcact gcaggatttc gaggaagcgg acctgatcct cgtcttcggg cagaaccccg
     3721 gcaccaatca tccgcgcatg ctcggcgaac tgcgccgggc ggtgaagcgc ggcaccagcg
     3781 tgcttgcatt caacccgctg cgcgagcgcg ggctggaacg cttcgccgac ccgcaggacg
     3841 cgctggagat gctccatggt ggttcgacga agattgcgtc cgactactac cagctacgca
     3901 tcggcggcga cctggccgcg gtcaccggca tcatcaagca cgtcttggaa cgcggcgcgc
     3961 gggcgcatgc cgaaggcaag ccagccctgc tcgatgaagg cttcatcgaa acccataccg
     4021 agggcttcga tgcgctactg gcaagcgtgc gcgccgccca ctggcggcag atcgaagcgg
     4081 aatccggatt gtcggaagaa cgcctgcgcg atgctggcga gcgcttcctg cgctccgaac
     4141 gcgccatcgt ctgctggggc atgggcatca cccagcacaa gcgagcggtt gcgaccatcc
     4201 agatgctggt caacctgatg ctgctgcgcg gccatatcgg ccgccccggc gcggggatct
     4261 gcccggtgcg cggccacagc aacgtgcagg gcgaccgcac catgggcatc cacgaaaagc
     4321 ccgcacccgc cctgctcgat cgtctgggcg aggtgttcgg tttcgagccc ccacgcgagc
     4381 atggcgtgga cgtggtcggc gcgatcgagg cgatgcgcga tggccgcggc aaggtgttct
     4441 tcgcgatggg cggcaacttc gccaccgcca cgccggatac cgaggccacc catgccgcgc
     4501 tgcgcaattg cgagctcacc gtgcacgtgg ccaccaagct caaccgcagc cacctggtcc
     4561 acggcaagga cgcgctgatc ctgccctgcc tgggccgcac cgagatcgac ctgcaggccg
     4621 gcggcccgca ggcggtgacg gtggaggact cgatgagcat ggtccacctg tcggcgggca
     4681 ttaacgcgcc ggcatcgccc gaattgctgt cggaaccggc gatcgtcgcg cggctggcgc
     4741 aggcgacgct gggcaagcgc agcgacacgc cgtggcgctg gctggtggaa gactacgatc
     4801 gcatccgcga ccagatcgca cgggtcttcg aggacttcca cgacttcaac cagcgcgtgc
     4861 gcgtgcccgg tggcttccgg ctcaccaaca ccgccgcgct tcggcaatgg aagaccgcga
     4921 ccggcaaggc gaacttcttc gcgcaccgac tggccgagga cgatccgcta cccgacatcc
     4981 ccggcgcgca actgttcacg ctcaccaccg tgcgctcgca cgaccagtac aacaccacca
     5041 tctacggcct gcaggaccgc tatcgcggcg tgcatggcga gcgcagggtg ctgttcgcgc
     5101 atgccgacga catcgcgggc ctcggcttcg cggccggcca gcgcgtcgat atcgaaagtg
     5161 tccacgacga cggcgtgcag cgcatcgtgc gcggcttcct gctggccgcc tacgacatcc
     5221 cgcgcggctg cctggcggcg tactaccccg agaccaatcc actggtgccg ctggacagtt
     5281 tcgccatcga ggcgcgtacg ccgaccagca aggccatccc ggtgatcctg catgcgcacg
     5341 atgccgcggc ggcccatgcg cagccgcgcg acatcgccgc cacgctggtc ggcaagaagc
     5401 gcgagaagcc ggcgctctct gcccgcaagc cgccgcagta gaaccgggtc agccggcagc
     5461 gagcacgtca gccgcggcca cgatgtcctc gtccttcggg atcaccagga acgcagcgcc
     5521 cgccagcggc gtgtaggtat cggcgccgca gacgcggcgg ccgggcttgc cgccgagtcc
     5581 cgcctcggtg atcgcggtga tcacgccctc gccgatgccg gcgctgaagc ggccctcgtc
     5641 gacgaccagg atgcgcttgc actcgcgcgc atgcctggcg atcgccttgt ggttgagcgg
     5701 caccagccag cgcaggtcga ccacgcgcac cttccagccg tgcttcgcct cgatctgccg
     5761 cgcagcgcgc aggctcatcg gcatcccgtt gccatagctg aagatcacgc aatcggtggc
     5821 gtcggcgttg tagacgcgct cctcgccgag caccagggcc tggtcgggcg ccgggtactc
     5881 ggtcagccac tggccatcgc cgggttcgta caagtctttc gtcatgtaca aagcgatcgg
     5941 ctccaggaac acgctgaccc ggccatcgac cttggccagc gcggtgagcg tgcgcagcat
     6001 catcgccgcg tcgtcgccgc gcgaggcgca acccaccacc aggccgggga tgtcgcgcag
     6061 cgcggtcatc gagttgtcgt tgtggaaatg cccgccgaag ccgcgctggt aacccaggcc
     6121 ggcgatgcgc accaccatgg ggttgcggaa ctggtcgttg ctgaagaact gcaggctggc
     6181 cgcctcgccg cggatctggt cgatcgcgtt gtggaggtag gccaggtact ggatttccgg
     6241 gatcggcagc atgcccatgt tggcgaagcc ctgggccatg cccaggatca tcgtctcgtc
     6301 gagcagcgtg ttgaacacgc gcttgttgcc gaaggccttg tgcaggccct tggtcaccgt
     6361 gtacacgccg cccttctgcg ccacgtcctc gccgaacagc agggtttccg gatacttggc
     6421 gaacaggtcg tgcaacgcct ggttgatctg gatggccaga tgcctgggca cctgcttctc
     6481 cggcagcttc tcctcgctgc cgaaggcctt gatgcgggcc tcctgcgacg gcacgcgggt
     6541 ggcttccgcc agcaccttgt ccggcgtgta gggcgcgagc gggcgcatca cgtgttccag
     6601 cgtctcgatg cgcgctcgct ggtcggcgtc ttccgcagcg gcgaaacact tcctgcgcgt
     6661 gtcttcgtac agcgcgagga tctcgtcctt cgacatcagt ccggattcca gcgcgatctg
     6721 cgcggaacgc agcagcggat cgcccgcctc caccgcgcac agttcctcga tgctgcgcca
     6781 ctcgatctcg aagtcggtac cggcgtggcc catgatccgg gtggtgcgca gatgcaggaa
     6841 ggtcgggcgg cgggtgcgcc ggcaatgctc gaccgcacgc tgcacgtcgg cgtagccgtt
     6901 ggccaggtcg aggccgtcgg cgaagaaata atccaggtcg gggcggttgc ggaagctttc
     6961 gccgatccag ccggtcggcg tcttcaccga gatgccgatg ccgttgtcct cgcagacgta
     7021 cagcaccggc gccggcagct tctggtaggc cgtccacgat gccgcattga atgcggtctg
     7081 cgcggtggca tggttggccg aggcatcgcc gaacgaacag atcgcgatgg aatcatcggg
     7141 aatcggcagc tggtgaccga tgcggcgtgc ggcttcgatc gcgaccgcgg tgcccagtgc
     7201 cttcggcagg tgggaggcga tcgtcgaagt ctgcggcagc acccacagcg gcttgctgcc
     7261 ccagaccttg tggcggccac ccgatgccgg gtcttccgcg ctcgccgcga agctcagcgc
     7321 cgagtccatg atcggatcca tgccgggcag cttgcggaag cgctcggcca tgaaaccgcc
     7381 actgcggtaa tgcaggaacg cggggtcggt atgacgcgcg gcgcgcgcga ccatggcatt
     7441 gccttcgtgg ccgcttgatc cgatggtgta gaagaccttg ttctgcacgc gcagcacgcg
     7501 cgccatcagg tcgagatggc gactgatcag ctgcgattcg aacaggtcgc ggaagccgcg
     7561 ggcatccagc gcgctgccct cgaggatcgc ttcgtccggt gccggcttcg catcggggat
     7621 gccgtcccag ctcttcacga actcgctgaa attgacgtcg cagacctcgg cgcggttgac
     7681 gcccttcatg cgcgcgggaa tgggattcgg gatggtggcg aacatggctt tctccccctc
     7741 tcccgcatgc gggagagggt tggggtgagg atgattcagg tcgactggtc gatcaatgct
     7801 tgcgcagccg catccggtgc aggcaaccgc acgaagccgg gggtggcttc cacgcacgac
     7861 agccatccgc gcagcgcggg atacgcgtcc agcgcgatgc cgccatgcgg tgcgacgcag
     7921 gtgtaggcat acagggcgat gtcggcgatg ccatagccat cgccggtgaa ccacgccttc
     7981 gtcgcaaggt gtcgttccat gaccgcgagt gccgcatggc cgcgttcgcg cagtttcggc
     8041 aggtcggcgc gacgcggcga atccgccggc gtccagccgc agatgaaacg cgcaacggcg
     8101 atgcagggtt catggctgta ctgctcgaag aacagccagc tgagtgcctg cgccttctgc
     8161 cacggatccg cgggcatgaa cgaggtgcca tcggccagcc agcacaggat cgcgttcgat
     8221 tcgaccagca cgcggccgtc gtcgagttcg atcatcggca ccttgccgtt cgggtttttc
     8281 gccaggtagt ccggggtgcg cgtcgcgccc ctgctgctgt cggtttccac ccagcggtag
     8341 tcgcgatcaa gacgaccgcc aagttgctcc agcagcagct tcaccttgtg gcaattgccg
     8401 gagggcgaaa agccatggac cgtgatcacg ccacgctccc cttgcgacgc gccacccatt
     8461 cctcccgggt ttgtccccag atatcgatca ccacgtcctg gaacggcggc ggcaggcttc
     8521 cgcgcccgcg attgcgcgac cccagccgct gcgccaacgc ctgcgatgcg gtgttgtcga
     8581 ggtcgatgga atggatcacc tcggtccagc caagctcggc gaaggcccag tcgatggcgg
     8641 ccgtcgccgc ttccagcgcg tagcccttgc cccacgcatc ccggtgcagc gcccagccga
     8701 cctcggtccc gggccatcct tcgggcttcc aagggccgat gttgccgatc cagttgccgc
     8761 tggccttctc gaccacggag aacatcgcga agccctgcac cgcccacgcc ccgggcattt
     8821 ccaggaaccg gcgccatgcg gctgcccgcg tcatcacccc accgatgtgg cgcgaggcct
     8881 cctcatccgc gcgaagttcg gcgaaacgct cgaaatcctc gatgcgttgc tggcgcagga
     8941 ccaagcgttc ggtttcgatg cggatatcgg agagcgacat atcggaagcc tccgctcgtc
     9001 attcccgcga aagcgggaat ccagcgcctg ggaacacagt cgaaaagcgg gatccccgcc
     9061 ttcgcgggga tgacgggcaa ggatcagaac gcgttgatgc cggtcagctc gcggccgatc
     9121 accaactggt gcacggtttc cgtgccctcg taggtgatga ccgattcgag gttcagcgcg
     9181 tggcggatcg cggcgtgctc ggtggtgatg ccggcgccgc caagcaggtc gcggcattcg
     9241 cgggcgatat cgatcgccat ccggcaattg ttccatttcg ccagcgagac ctgcgcgggc
     9301 tgcatggtgc cggcatcctt caggcggccc aactgcagca ccagcatctg cgccagggtg
     9361 atgcggcggg ccatgtcggc catcttgatc tgcgcgctct gcgtcgccgc gaccgggcgg
     9421 tcgaacagga tgcgctcctt ggtgtatttc agcgcttcat ccaggcaggc gatggcggcg
     9481 ccgatcggac cccaggtaat gccgtagcgc gcctgggtca ggcagcccag cggacccttc
     9541 aggcccttca cgttgggcag gcgattggct tccggcacgc gcacgttgtc gaagaacagg
     9601 gcgctggtca ccgacgcgcg caggctcatc ttcttgtgga tttcctgcgc ggtgaacccg
     9661 gcgaagccct tctccaccag gaacccctgg atgccgtcgt ccgtctgtgc ccagacgatc
     9721 gcgatgtcgg ccaggttgcc gttggtgatc cacatcttgg acccgttgag gatccagtcg
     9781 tccccgtcgc ggaccgcgcg ggtcttcatg ttggccgggt cggagccgcc ctgtggttcg
     9841 gtcaggccga agcagccgat caccttgccc gccgccatct gcggcaacca gcgcatgcgc
     9901 tgctcctcgg taccgtaggc atagatcggg tacatgcaca gcgagctctg cacggacacg
     9961 aagctgcgga tgccgctgtc gccgcgttcc agttcctggc agatcaggcc gtaactgacg
    10021 gcgttgaggc cggcgcagcc gtatttttcg gggagcgagg agccaagcag ccccatctcg
    10081 gcgatctcgg caaccagttc cttcgggaac ctgccctggt cgaaggcatc gccgatgatc
    10141 ggaatcaccc gctcgtcggt aaagcgtgca acggcgtcct gcaccgcgcg ctcttcttcg
    10201 gtcaacaggg aacggacatc gaacagatcg taaggaggca aggccatggc ggtcgcgtcg
    10261 gggctgtcaa accgccattg tagtcgtgca ggccgcgcaa ccggcacctg cgatgtgacc
    10321 agcaagcacc gccgaaacgc cgcggtttca gccaaagcca tgcgtttcga catcggcacc
    10381 gccagcccgc ccgcaaaagt tgacggggcc ggattgctcc ggccccgcct gggccttgcg
    10441 cggttggtac agtcccgcgg tcagccgttc ggttcgcccg tggtcgacgc cacttgcgtc
    10501 accacttcgc catcgagcaa tggcacacgc tggccgggcg gatgcgccat ccgcacggtg
    10561 cggatatccc cgtccagcat gtagcgcacg tcataggcga cggtgcggtc atcgctgccg
    10621 agggcaatgc gttcgcccgg cttgcgctcg gtctgcaggg tgccggtggt gccgtccgca
    10681 ttccggtacg tcacgttcca ggcgatcgtg cgcgacgagg tcgaggtatt gttgacggtc
    10741 ctgcagcggc gatccacgcg actcaccacc tggccaccga cgtggttgcg gtcgacgcgg
    10801 ttgccgatga agccgcccgc caccgcaccc gcggcggtcg cggccttgcg gccattgccg
    10861 ccgcccacct ggttgcccac cagtccaccg atcagcgcgc cagccacggt gccgccgacg
    10921 ttgccgtcgc gctctggcat gcgttcttgc acggccacgt ctgcgcagac ctgctgcggg
    10981 gtggtggtgg agctggattc gcggaccgga tcggcgccga tgacggtggc gtagcgctct
    11041 tcgcgctcga tcacgggttc gacactgacc acgtctgcat aagccaagcc cacggcgcca
    11101 tcgtcggtgc cgcgataagc gctgtcgacc gccatcggat tggtcgaccc acgggcatta
    11161 ttgttgttga tgaagccggc ggtggccacg ccacccacca gcagcgcgcc gagcgcgatc
    11221 gcaagcgtat tgcccttcaa catggtctcg cctcctgcgt catgtgacgg ggacggattg
    11281 cagcatggcg agtctgaaca cccacggagc caccgcagcg cgcatcggca agcgttcacg
    11341 cttgtgcaga acgcgtgcat gctagcgttc gcggcaatcc acaaggatcc tgttcatgcc
    11401 caatcccctt ctcctgccgc tgctcaactg ggcgcgcaaa ctccgctacc cggtgctgtt
    11461 caagctgacc gcagcgctgt tcgcgatcag cgtcctgttg ccgccgggca tcgatccgat
    11521 cccgttcctg gacgaaatcg tgttcggcct cggcacgctg ctgctcgcca actggaaaac
    11581 gcgcaagcca cccgcaggca acgagaagcc acccatcgac ggcgaagccc gtcgctgaac
    11641 ccatgctggt cgacagccac agccatttcg acgcaccgga gttcgatgcc gatcgcgctg
    11701 ccgcgcatgc gcgtgcgctc gccgccggcg tcgacatcca gatcgtgccc gcggtcgaag
    11761 ccgccacctg gccgaaactc aaggcggtct gcgcggaatt ctcgggattg catgcggcgt
    11821 atggcctgca cccgatgtac ctggacgcgc accggcccgc gcacctcgat gacctacgca
    11881 actggatcga gcgcgagcgc ccggtcgcgg tcggcgaatg cgggctggac tatttcgtcg
    11941 aaggcctgga ccacgaggcg cagcagcttt ttttcgacgg acagctgcga ctcgcgcgcg
    12001 acttcgacct gccggtgatc gtgcacgcgc gccgcgccgt ggatgcggtg atcgcctcgc
    12061 tccgcaaggt cggcgggctg cgcggcgtgg tgcacagttt ctcgggcagc gccgagcagg
    12121 cg
//
LOCUS       DAOOCX010000070        11930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5005_length_11930_cov_7.640000, whole genome shotgun sequence.
ACCESSION   DAOOCX010000070 DAOOCX010000000
VERSION     DAOOCX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11930
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5005_length_11930_cov_7.640000"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="PK399_07860"
     CDS             complement(<1..164)
                     /locus_tag="PK399_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW13072.1"
                     /translation="MSAASADDRLDVLVVGAGPAGLTAATYLARFHRRFALVDAGKSR
                     ARWIPTSHNCP"
     gene            complement(145..759)
                     /gene="folE"
                     /locus_tag="PK399_07865"
     CDS             complement(145..759)
                     /gene="folE"
                     /locus_tag="PK399_07865"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPW13073.1"
                     /translation="MADNDHRTDDVRNAVSRAQAEDAVRTLLRWSGDDPDREGLLDTP
                     KRVAKAYEDWFSGYALDPDAYMARTFEEVAGYDEMIVLRDIEYESHCEHHMAPIIGKV
                     HVGYLPAGKVVGISKLARVVEAYARRFQVQEKMTSQIANCIQRALQPRGVGVVVEGAH
                     ECMTTRGIHKRGVSMVTSKMLGGFRDDARTRSEFLRFIDVGGKR"
     gene            complement(829..>1857)
                     /locus_tag="PK399_07870"
     CDS             complement(829..>1857)
                     /locus_tag="PK399_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPW13074.1"
                     /translation="EVDLFGRVRRGVEAANADVAASQADLRAMQVAIVGDLARSYVGL
                     RGLQERARVARANADNQRETLTLVQARFDAGRDTAFDTARARAQREATLAQVAALDAE
                     VGIAIHRIAVLAGRMPDALDAELSAVAPLPAVPAAPDPGTPGELLRRRPDVAAAEQRL
                     HAATARIGVATADLFPRFTLGALIGSQAATGSALFGRDSETGLVALGIDWSFLDTGRV
                     RARIGAADADAQAALAQYQRSVLLSLRDTGDALLRLDQARIEDTHLQQAASDSADAAR
                     LARLRYQAGASGLLEVLDAERTRLSAEDAFAAARTRSVERAIALHQALAGGWPRQLPR
                     RVGLAEAD"
     assembly_gap    1858..1957
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1958..2304)
                     /locus_tag="PK399_07875"
     CDS             complement(<1958..2304)
                     /locus_tag="PK399_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW13075.1"
                     /translation="MVIRPLALALATALLSACAVGPDFVRPELAVPAQFEHATAANAA
                     TIADDAGFWRGFDDPLLTELVEEALAANHDLRIALANVDRAEALRRGSRLDALPRVTA
                     DADVSGSRASADQL"
     gene            complement(2298..3050)
                     /locus_tag="PK399_07880"
     CDS             complement(2298..3050)
                     /locus_tag="PK399_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW13076.1"
                     /translation="MNTNTNKIALVTGATRGIGLETVRQLAETGVHVLLAGRNRDKAV
                     EAALQLQRQGLLVEAIALDVTDSASIVAAAKEIEQRHGKLDILVNNAGILVDDAEKGV
                     SGQSLATWRTTFDTNLFGLIETTQALLPLLRKSDAGRIVNVSSLLGSISEHQNPASFI
                     YEFKGVPAYNVSKSAVNAWTVHLAHELKDSGIKVNTIHPGYVQTDMNKSGDQQNGELS
                     VPEGARTSVQLALIGNDGPTGGYYYFDQVLPW"
     gene            complement(3123..6293)
                     /locus_tag="PK399_07885"
     CDS             complement(3123..6293)
                     /locus_tag="PK399_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HPW13077.1"
                     /translation="MDFSRFFIDRPIFAAVLSIVIFAAGLIAIPLLPIGEYPDVVPPS
                     VVVRTVYPGANPKVIAETVATPLEEAINGVEDMMYFKSVAGSDGVLQLTVTFKPGTDA
                     DEAAVRVQNRVAQALARLPEDVRRQGVTTQKQSPVFLMVVHLVSKDGKYDSLYLRNYM
                     RLHVKDELAKISGVGDAQLFGGGDYAMRLWLDPDKIAARGLTAGDVLRAVREQNVQVS
                     AGQLGAEPMPNGSDFLLPINAKGRLESVAEFGDIVLESGTGGEIVRLRDVARIELAAG
                     DYTLRARLDGKNAAAIGIFQAPGANALQIRDAVVAKMDELRPTLPPGVELQSIYDTTI
                     FVRDSIKAVVTTLLEATLLVVLVVILFLQTWRASIIPLLAVPVSVVGTFAVLHLLGYS
                     INTLTLFGLVLAIGIVVDDAIVVVENVERHIEHGASPLEAAHLAMREVSGPIIAIALV
                     LCAVFVPMAFMTGVTGQFYKQFAVTIAISTVISAINSLTLSPALAAKLLKPRDAAKDA
                     PSRLIDRLFGWLFRPFNRFFKRNSERYEGTVSRALGRRGAVFVVYAVLLVGAAFMFRI
                     VPSGFIPVQDKLYLIAGVKMPEGASIERTDAALKKMAAIAKGVDGVAHEVAFPGLNPL
                     QFTNTPNSGVVFFTLDPFNERSRSAAEITAELNQKFSSIQEGFTFAFMPPPIQGLGNG
                     SGWSLFVEDRTRLGYGELQGAVQAFQGAASQAPGLGFPISSYQANVPQLDVEVDRVKA
                     KAQGVPLTELFDTLQAYLGSAYVNDFNMFGRTWQVIAQADGPFRDNVEDIANLRTRNA
                     AGEMVPLGSMVQVKQSYGPDPVIRFNGYPAADLLGEADPRVLSSGEAMAKVTALAQQV
                     LPTGMAIEWSDLSYQQATQGKAAMVVFPLAVLLAFLVLAALYESWTLPLAVILIVPMT
                     LLSALFGVWLAGGDNNVFVQVGLVVLMGLACKNAILIVEFARELELQGKGIVESALEA
                     CRLRLRPIVMTSVAFIAGTVPLVLSHGAGAEVRSITGITVFAGMLGVTLFGLFLTPVF
                     YVALRKLANRPLVSHAPHAIAAPAHA"
     gene            complement(6439..7581)
                     /locus_tag="PK399_07890"
     CDS             complement(6439..7581)
                     /locus_tag="PK399_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW13078.1"
                     /translation="MHPMLAGLAIAVLVALAASACSSEAAPAAGPPPPEVSVASVLTK
                     DVSQWEDFTGRVAAVESVELRPRVSGYVQRVAYAEGEEVRKGDLLFVVDPRPYQAALD
                     RALAELARAQAESRLAGAQDARAQSLIEVKAISREEFEQRKANTAGANAAVRAAEAAV
                     TAARLDLQFTQVRSPIDGRAGRALVTQGNLAQADATLLTTVVSQDPVFVYFEADEQTW
                     LRHSAAARSGDNPVRVGLSSETGYPHTGTLDFIDNQVDPRTGTLRARAVLRNPDRLFT
                     PGLFARVQLQGSGQAAALLVDDKAVLTDQDRKYVYVVGADNTAQRKDVVLGRSVDGLR
                     VIQSGLAPRDRVIVQGVQKVFFPGMPVLPKQVAMGAAAPAPTVAMK"
     gene            complement(7656..8456)
                     /locus_tag="PK399_07895"
     CDS             complement(7656..8456)
                     /locus_tag="PK399_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW13079.1"
                     /translation="MPLATRRAAVVVLDASGVIGRGLLAAALDAGHPVVAVDRNPATL
                     AALAAEHPRGSLSVLAGSAAGEADARALATALRGLGHPLAAVLSAVDASGLRGRLLDQ
                     PADATCNALQCALEPQLAAVRHLLPLLHHGGRYVLVGGPGGQHPWAGQGQRSLVEAAL
                     RMLARVLHDEARAVGTRLQLLSVATPTCGLHAGAPRPHWPTALQIGQRAIQLLGDRHG
                     EPVVDFALAPLDPPVVANTSPAGCLRDARALLGRLTAAATHSYKEASP"
     gene            complement(8480..8725)
                     /locus_tag="PK399_07900"
     CDS             complement(8480..8725)
                     /locus_tag="PK399_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13080.1"
                     /translation="MRSNSASRLRRHALLGFLAVDMLGLAALLLDQVFSTDPVYPWII
                     AMGLVIAALPLLAQAVDYVLSTPAEAAIVPDAGEKIP"
     gene            8855..9775
                     /locus_tag="PK399_07905"
                     /pseudo
     CDS             8855..9775
                     /locus_tag="PK399_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10046..11929
                     /locus_tag="PK399_07910"
     CDS             10046..11929
                     /locus_tag="PK399_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HPW13081.1"
                     /translation="MPPVLSRTALALAVALGLGLSACSKPATDAAPATAATAAPSAEQ
                     IKAESARLNAWFDAQYEEQLKFSPIQLSFQGRKELNDQLDDMSEAGARKQEAWMAASV
                     KAMEAGFDYDKLDAETRLSWDLWKRQYENARDGLPFLASGYAFDQMNGAQSFLPTFLI
                     NFHKVDDEKDYLAYVSRLQKVPVAFDQLLERAQAGIAKGVRPPKFAYEGVIEQSRKVI
                     AGAPFSGGADSALWADAQAKADTLAKDGKVTAERAAELKALARTALTDNVKPAYDKVI
                     AFATGDLPNAKVDPAGIGSTQADGAAYYTYQLKQNTTTAMSADEIHQLGLSEVARLRG
                     ELEKVKATVGFEGDLPAFFKHVQGDPNQLFSNDDAGRKAYIDEATRVIGNIKEHLPDY
                     FGLLPKADLVVKRVEPFREQPGAAQHYYPSTPDGSRPGVYYAHLSDMKSMPKTTLEVI
                     AYHEGLPGHHMQIAIAQELKGVPVFRTQFGSTAYAEGWGLYAEALAKEMPGTYQDPYS
                     EYGRLTSEMWRAIRLVVDTGMHAKGWTEQQAVDYFRANSSIPDGAIKSEVRRYLIMPG
                     QATAYKVGMIKIQQLRKKAETELGEQFDIRGFHDAVLGGGALPLDLLERRVDSWIAGK
                     KAG"
BASE COUNT         1982 a         4077 c         4025 g         1746 t
ORIGIN      
        1 gggcagttgt ggctggtcgg gatccagcgc gcgcggctct tgccggcatc gaccaacgcg
       61 aagcggcggt ggaagcgcgc aaggtaggtc gcggcggtca gcccggcagg accggcgccg
      121 accaccagca cgtcgagccg gtcgtcagcg cttgccgccg acatcgatga agcgcaggaa
      181 ttcgctgcgg gtgcgcgcgt cgtcgcggaa gccgccgagc atcttggagg tcaccatcga
      241 cacgccgcgc ttgtggatgc cgcgcgtggt catgcactcg tgcgcgccct ccaccaccac
      301 gcccacgccg cgcggctgca acgcgcgctg gatgcagttg gcgatctgcg aggtcatctt
      361 ctcctgcacc tggaaacgcc gcgcgtaagc ctcgaccacc cgcgccagct tgctgatgcc
      421 gacgaccttg cctgccggca ggtagccgac gtggaccttg ccgatgatcg gcgccatgtg
      481 gtgctcgcaa tggctttcgt attcgatgtc gcgcagcacg atcatctcgt cgtagccggc
      541 gacttcctcg aaggtgcgcg ccatgtacgc atccgggtcc agcgcgtaac cgctgaacca
      601 gtcctcgtag gccttggcca cgcgcttggg ggtgtccagc aggccctcgc gatcgggatc
      661 gtcgcccgac cagcggagca gggtgcgcac cgcgtcctcg gcctgcgcgc ggctgaccgc
      721 gttgcggacg tcatcggtgc ggtggtcgtt gtcggccatg gcggttccgg cttgcgtggg
      781 gataggcatg ttagcgaacc gcaggcgatg cggcgtcgaa gcggtggctc agtccgcctc
      841 ggccaggccg acacggcgcg gcagttgccg cggccaaccg cccgccagcg cctggtgcag
      901 ggcgatcgcg cgttccaccg agcgcgtgcg cgccgcggcg aaggcgtcct cggcggacag
      961 gcgggtgcgc tcggcatcca gcacctccag caagccggac gcaccggcct ggtagcgcag
     1021 ccgcgccagc cgcgccgcgt cggcgctgtc gctcgccgcc tgctgcaggt gggtgtcctc
     1081 gatgcgtgcc tggtccagcc gcaacagcgc atcgccggta tcgcgcaacg acagcagcac
     1141 gctgcgttgg tactgcgcca gcgccgcctg cgcatcggca tcggcagcgc cgatgcgggc
     1201 gcgcacgcga cccgtatcca ggaaactcca gtcgatgccc agcgcgacca gcccggtttc
     1261 gctgtcgcgg ccgaacaacg cgctgccggt cgccgcctgc gagccgatca gcgcgcccag
     1321 ggtgaagcgc gggaacaggt cggcggtggc cacgccgatc cgtgctgtgg ccgcgtgcag
     1381 gcgttgctcg gcggcggcca cgtcgggacg tcggcgcagc agctcgccgg gggttcccgg
     1441 atccggcgcc gccggcaccg cgggcagcgg tgcgacggcg gacagttcgg catccagcgc
     1501 atcgggcatg cgcccggcca acaccgcgat gcgatggatg gcgatgccga cctcggcgtc
     1561 cagtgccgcc acctgcgcca gcgtggcctc gcgctgcgcg cgggcacggg cggtgtcgaa
     1621 cgcggtgtcg cggccggcgt cgaagcgcgc ttgcaccaat gtcaatgtct cgcgctggtt
     1681 gtcggcattc gcacgggcga cgcgcgcccg ttcctgcaac ccgcgcaagc cgacatagct
     1741 gcgcgcgagg tcgccgacga tggcgacctg catcgcgcgc aaatccgctt gcgatgcagc
     1801 aacatcggca ttggcggcct cgacgccgcg acgcacgcgc ccgaacagat cgacttcnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt tgatcggcgc tggcccggct
     1981 gccggataca tcggcatcgg ccgtcacccg cggcaaggca tcgagccggc tgccgcgacg
     2041 cagggcctcg gcacgatcga cattggccag cgcgatgcgc aggtcgtggt tggccgccag
     2101 ggcctcttcg accaactccg tcagcagcgg atcatcgaag ccgcgccaga agccggcatc
     2161 gtcggcgatg gtcgccgcgt tggcggccgt cgcatgctcg aactgcgcag gcaccgcgag
     2221 ttcgggccgc acgaagtccg ggccgaccgc gcacgccgac agcagcgcag tcgccagcgc
     2281 caacgcgagc gggcggatca ccatggcagc acctggtcga agtagtaata accaccggtg
     2341 gggccgtcgt tgccgatcag ggccagctgc acgctggtgc gcgcgccttc cggcaccgag
     2401 agctcgccgt tctgctggtc gccggacttg ttcatgtcgg tctgcacgta gcccggatgg
     2461 atggtgttga ccttgatgcc gctgtccttc agttcgtgcg ccaggtgcac cgtccacgcg
     2521 ttcaccgcgc tcttcgacac gttgtaggcc ggcacgccct tgaactcgta gatgaaggac
     2581 gccgggttct ggtgctccga gatcgagccg agcaagctcg agacgttgac gatgcggccg
     2641 gcgtcggact tgcgcagcag gggcagcagc gcctgggtgg tttcgatcag gccaaacagg
     2701 ttggtgtcga aggtggtgcg ccaggtggcg agcgactgcc cggagacgcc tttctcggca
     2761 tcatcgacca ggatcccggc gttgttgacc aggatgtcca gcttgccgtg gcgctgttcg
     2821 atctccttcg ccgcggcgac gatgctggcg ctgtcggtga catccagcgc aatggcttcg
     2881 acgagcaggc cttgccgctg cagctgcaat gccgcttcga cggccttgtc gcggttgcgg
     2941 cctgcgagca ggacgtgcac cccggtttcg gcaagttggc ggacggtttc caggccgatg
     3001 ccgcgggtgg cgccggtgac gagggcgatc ttgttcgtat tggtgttcat ggctttgcct
     3061 tgtgtgggga gaaattcgtc aggagaaagt ggaagaagga gagatcaggg aatcgacatc
     3121 gatcaggcat gcgccggagc cgcgatcgca tgcggcgcgt gcgagaccaa cggacggttg
     3181 gccagcttgc gcagggccac gtagaacacc ggggtcagga acaggccgaa cagggtcacg
     3241 ccgagcatcc cggcgaacac ggtgatgccg gtgatcgagc gcacttccgc accggcgcca
     3301 tgcgagagca ccagcggcac ggtgccggcg atgaacgcga ccgaggtcat cacgatcggg
     3361 cgcaggcgca ggcggcaggc ctccaatgcg gactcgacga tgcccttgcc ctgcagttcc
     3421 agctcgcgcg cgaactcgac gatcaggatc gcgttcttgc acgccagccc catcagcacg
     3481 accaggccga cctgcacgaa cacgttgttg tcgccgcccg ccagccacac gccgaacagc
     3541 gcggacagca gggtcatcgg cacgatcagg atcaccgcca gcggcagggt ccagctttcg
     3601 tacaacgcgg ccagcaccag gaaggccagc agcaccgcca gcgggaacac caccatcgcc
     3661 gccttgccct gggtggcctg ctggtagctc aggtcgctcc attcgatcgc catgccggtg
     3721 ggcagcacct gctgagcgag tgcggtgacc ttggccatcg cctcgccgga ggacagcacg
     3781 cgcgggtcgg cttcgcccag caggtcggcg gcgggatagc cgttgaagcg gatgaccggg
     3841 tcggggccgt aactctgctt gacctgcacc atgctgccga gcggcaccat ctcgccggcg
     3901 gcgttgcggg tgcgcaggtt ggcgatgtcc tccacgttgt cgcggaacgg accatcggcc
     3961 tgggcgatca cctgccaggt gcggccgaac atgttgaagt cgttgacata ggcggagccc
     4021 aggtacgcct gcagggtgtc gaacagctcg gtgagcggca cgccctgcgc cttcgccttg
     4081 acccggtcga cctcgacatc cagctgcggc acgttggcct ggtagctgct gatcgggaag
     4141 ccaagcccgg gtgcctgcga ggccgcgccc tggaacgcct gcaccgcgcc ctgcagttcg
     4201 ccgtagccca gccgcgtacg gtcttcgacg aacagcgacc aacccgagcc gttgcccagc
     4261 ccctggatcg gcgggggcat gaaggcgaag gtgaagcctt cctggatcga cgagaacttc
     4321 tggttgagct cggcggtgat ctccgccgcg ctgcgcgagc gctcgttgaa aggatccagg
     4381 gtgaagaaca ccacgccgct gttcggcgtg ttggtgaact gcagcggatt cagcccgggg
     4441 aaggcgacct cgtgggccac gccgtccacg cccttcgcga ttgccgccat cttcttcagc
     4501 gcggcatcgg tgcgctcgat cgaggcgcct tccggcatct tcaccccggc gatcaggtac
     4561 agcttgtcct gcaccgggat gaagccggac ggcacgatcc tgaacatgaa ggccgcgccg
     4621 accagcagca ccgcgtacac cacgaacacc gcaccgcggc gacccagcgc gcgcgacacc
     4681 gtgccttcgt agcgttccga gttgcgcttg aagaagcggt tgaacgggcg gaacaaccag
     4741 ccgaacagcc ggtcgatcag gcgcgacggc gcgtccttcg ccgcatcgcg cggcttcagc
     4801 aacttggcgg ccagcgccgg cgacagggtg agcgaattga tcgccgagat caccgtggaa
     4861 atcgcgatgg tcaccgcgaa ctgcttgtag aactggccgg tgacgccggt catgaacgcc
     4921 atcggcacga acaccgcgca caacaccagg gcgatggcga tgatcggccc ggagacctcg
     4981 cgcatcgcca ggtgggcggc ctcgagcggc gacgcaccgt gttcgatgtg gcgctccacg
     5041 ttctcgacca ccacgatcgc gtcgtcgacc acgatcccga tcgccagcac caggccaaac
     5101 aacgtcagcg tgttgatcga atagccaagc aggtgcagca ccgcgaacgt gccgaccacc
     5161 gacaccggca ccgccagcag cgggatgatc gaggcgcgcc acgtctgcag gaacaggatc
     5221 accaccaaca ccaccagcaa cgtcgcttcc agcaaggtgg tcaccaccgc cttgatcgaa
     5281 tcgcgcacga agatggtggt gtcgtagatc gactggagtt ccacgcccgg cggcagggtc
     5341 ggacgcagtt cgtccatctt cgcgaccacc gcatcgcgga tctgcagcgc attcgcgccc
     5401 ggtgcctgga agatgccgat cgccgccgcg ttcttgccgt cgaggcgtgc gcgcaaggtg
     5461 tagtcgccgg cggccagctc gatgcgggcg acgtcgcgca ggcgcacgat ctcgccgccg
     5521 gtgccgctct ccagcacgat gtcgccgaac tcggccacgc tctccaggcg acccttcgcg
     5581 ttgatcggca gcaggaaatc gctgccgttc ggcatcggct ccgcgccgag ctggccggcc
     5641 gagacctgca cgttctgttc gcgcaccgcg cgcagcacgt cgccggcggt caggccgcgc
     5701 gcggcgatct tgtcggggtc cagccacagc ctcatcgcgt agtcgccgcc gccgaacagc
     5761 tgcgcatcgc ccacgccact gatcttcgcc agttcgtcct tgacgtgcag gcgcatgtag
     5821 ttgcgcaggt acagcgagtc gtacttgccg tccttcgaca ccaggtgcac caccatcagg
     5881 aacaccggcg actgcttctg ggtggtcacg ccctgccggc gcacgtcctc gggcaatcgg
     5941 gccagcgcct gcgccacgcg gttctgcacg cgcaccgcgg cttcatccgc atcggtgccc
     6001 ggcttgaagg tcacggtcag ctgcagcaca ccgtcggaac cggcgacgga cttgaaatac
     6061 atcatgtcct cgacgccgtt gatcgcctct tccagtggcg tcgccacggt ctcggcgatc
     6121 accttgggat tggcacccgg gtacaccgtg cgcaccacca ccgacggcgg caccacgtcc
     6181 gggtattcgc cgatgggcag gagcgggatc gcgatcaggc cggcggcgaa gatcacgatc
     6241 gacaacacgg cggcgaagat cggccggtcg atgaagaagc gggagaaatc catgcgggtt
     6301 actcggaaag tgggctctac tcctcccctt gcgaatgcag ggggaggtcg ggagggggct
     6361 gcttttcgtc gggaggattt gcgcaagcgc caacccctcc cctgccctcc cctgcacgca
     6421 ggggagggag tgagagcgct acttcatcgc caccgtcggc gctggcgcag ccgcgcccat
     6481 cgccacctgc ttcggcagca ccggcatgcc cgggaaaaac accttctgca cgccctgcac
     6541 gatcacccgg tcgcgcggcg ccaggcccga ctggatcacc cgcaggccat ccacgctgcg
     6601 ccccagcacg acgtccttgc gctgcgcggt gttgtccgca ccgaccacat agacgtactt
     6661 gcgatcctgg tcggtcagca ccgccttgtc gtcgaccagc agtgctgcgg cctggccgct
     6721 gccctgcagt tgcacgcggg cgaacaggcc gggggtgaac agccggtcgg gattgcgcag
     6781 caccgcgcgc gcgcgcagcg tgccggtgcg cggatcgacc tggttgtcga tgaagtccaa
     6841 ggtgccggtg tgcggataac cggtctcgct ggacaggccc acgcgcaccg ggttgtcgcc
     6901 gctgcgggcc gccgccgaat ggcgcaacca ggtctgttcg tccgcttcga aatagacgaa
     6961 caccggatcc tgcgagacca ccgtggtcag cagggtggcg tcggcctggg cgaggttgcc
     7021 ctgggtcacc agcgcgcgac cggcgcggcc atcgatcggc gagcgcacct gggtgaactg
     7081 caggtccagg cgcgccgcgg tgaccgcggc ttcggcggcg cgcacggcgg cattcgcgcc
     7141 ggcggtattc gccttgcgct gctcgaattc ctcgcgcgag atcgccttca cctcgatcaa
     7201 cgattgcgcg cgggcgtcct gcgcgccggc caggcgggac tcggcctgtg cacgggccag
     7261 ctcggccagt gcgcgatcca gcgcggcctg gtacggacgc ggatcgacca cgaacagcag
     7321 gtcgcccttg cggacttcct cgccttccgc atacgccacg cgctgcacgt aaccgctgac
     7381 ccgcgggcgc agctccacgc tttccaccgc ggcgacgcgg ccggtgaagt cctcccactg
     7441 gctcacgtcc ttggtcagca cgctggccac gctgacttca ggcggtggtg gcccggcggc
     7501 cggcgcggct tcgctgctgc aggcggaggc cgccagcgcg accagcacgg cgatcgccag
     7561 gccggccaac atggggtgca tcgacgcgcg caaggcgggc gcccgcggca cgccggagcg
     7621 gatcgcctcg gggaaggcag ggagacgacg gctgttcatg gggaggcttc cttgtacgaa
     7681 tgggtggccg ccgcggtcag gcggccgagc agggcgcgtg catcgcgcag gcaacctgcc
     7741 ggggaggtat tggcgacgac gggtggatcg agcggtgcca gggcgaaatc gacgacgggt
     7801 tcgccgtggc ggtcgcccag cagctggatc gcgcgctggc cgatctgcaa cgcggtcggc
     7861 cagtgcgggc gtggcgcgcc ggcatgcagg ccacaggtcg gggtggccac cgacagcagt
     7921 tgcaggcggg taccgacggc gcgggcttcg tcgtgcagga cgcgggcgag catgcgcagc
     7981 gcagcctcga ccagggagcg ttggccctgg ccggcccatg gatgctggcc gccgggacca
     8041 ccgaccaaga cgtagcggcc gccgtgatgg agcaatggca gcaggtggcg cacggcggcg
     8101 agttgcggtt ccaacgcgca ctgcaaggcg ttgcaggtgg catcggcggg ctggtcgagc
     8161 aggcggccgc gcaggccgct ggcatcaacc gcggacagga cggcggcgag cgggtgaccc
     8221 agtccgcgca acgcggtggc cagcgcgcgc gcatcggcct cgccggcggc ggaacccgcc
     8281 agcaccgaca ggctgccgcg cggatgttcg gcagcgaggg cggccagcgt ggccgggttg
     8341 cgatccaccg cgaccaccgg gtggccggca tccagcgccg ccgccagcag gccacggccg
     8401 atcacgccgc tggcatcgag caccaccacc gcggcccggc gggtcgcgag tggcattgtc
     8461 ccgcttgcca ttatcccgct cacgggattt tttctcccgc atccgggacg atggcggctt
     8521 cggcgggggt gctcagcacg tagtcgaccg cttgcgcgag cagcggcagc gcggcgatca
     8581 ccaggcccat cgcgatgatc cacgggtaca ccggatcggt gctaaatacc tgatccagaa
     8641 ggagcgctgc cagtccgagc atgtcgacgg caaggaaacc gaggagggcg tggcgacgca
     8701 ggcgggaggc ggaattggaa cgcatgacac ggctcgctgg gggaggagtg gcgccaaggt
     8761 acgcgccgac gcggcacttg attagtcgct gaatcgggga ataattgtcc cttcattggg
     8821 ccaatcaccc gccactcccg caaaggcgcc cgccatgaca cgcgatctca acgacaccct
     8881 cgtcttcgtc cgcgtggtgg agcacggcag cttcatcgcc gccgcgcgtt cgctgcagct
     8941 gcccaagacc acggtcagcc gcaaggtgca ggacctggag acgcgcctgg gcgcgcagct
     9001 cctgcatcgc accacgcgca agctcggcct gaccgaggcc ggcaacatct acttcgagca
     9061 ttgccagcgc atcgcccgcg aactggacga ggcggaaagc gcggtcggcc agttgcagca
     9121 gggcccgcgc ggctggttgc ggatcaccgc gccgtattcg ctcggcatcg aacgcattgc
     9181 gcccttgctg ggtgaattcc acacgctgca tcccgacgtg cgcgtggaga tgctgctgac
     9241 caacgagaac gtcgacatca tcgacaagga actcgacatc gcgctgcggg tggggccgtt
     9301 gccggattcg accttcatcg cgcgttcgct ggccacgctg cgcacccagg tgttcgccag
     9361 cgtcgcctac ctcgagcgcc acggcgaacc gttgcacccc gacgacctgc agcaccaccg
     9421 caccctggcg atgaccaagc agcgccgcaa cggtggtttc tgctggccgc tggacgatgg
     9481 cggcggcatg agcgaattcc gcatcgaccc ggtgttcgtc gccaacgatc cggccggcct
     9541 gcgcggcgca ctgctgtgcg gcgagggcct gatgctggcc aacgacgtga tggtcaagcc
     9601 gttcgtcgaa ggcggctacc tgcgccgcgt cctgccggcc tggaacggcc cggagtacca
     9661 gctcaacgcg gtgttcccac gcggtcgcgt gcagtcgccg aaagtgcgcg ccttcatcga
     9721 tttcctgatg gagcgcttcg actacgacgc cgactacatg cagctgcagt gcccggacaa
     9781 gcgccgcgag tgccagcagg cgcaacaccg cgccgccgat gccgcgcgcg ccgaactcca
     9841 gcgcgcgtcg cgcatcggcg acacgttgcg cgacgccgac atcgcaccgc gggagcccgt
     9901 gctcgcctga gccgggcgtt gccgccatga aacgtggccc aggccacgtt tcgatgccgc
     9961 catgcgcacg gcgtgaccat ccgcatgggc agcaacggcc cgcaggccac acaatcgggt
    10021 tgccttctcc cccaaggaac ccctgatgcc gcctgtcctg tcacgcactg ccctcgccct
    10081 tgccgttgca ctgggcctcg gcctgtccgc ctgctcgaag ccggccaccg atgccgcgcc
    10141 ggcaacggcc gccactgccg cgcccagcgc cgaacagatc aaggccgagt ccgcacgcct
    10201 caatgcgtgg ttcgacgcgc agtacgagga acaactgaag ttcagcccga tccagctcag
    10261 cttccagggc cgcaaggagc tcaacgacca actggacgac atgtccgagg ccggcgcgcg
    10321 caagcaggaa gcgtggatgg ccgcctcggt caaggcgatg gaagccggct tcgactacga
    10381 caagctggac gcggagacac ggctgtcctg ggacctgtgg aagcgccaat acgagaacgc
    10441 ccgcgacggc ctgcccttcc tcgccagcgg ctacgccttc gaccagatga acggtgcaca
    10501 gagcttcctg cccaccttcc tgatcaactt ccacaaggtc gacgacgaga aggactacct
    10561 ggcctacgtg tcgcgcctgc agaaggtgcc ggtggcgttc gaccagttgc tcgaacgcgc
    10621 gcaggccggc atcgccaagg gcgtgcgccc gccgaagttc gcctacgaag gcgtcatcga
    10681 acagtcgcgg aaagtcatcg ccggcgcgcc gttctcgggc ggcgccgaca gcgccctgtg
    10741 ggccgacgcg caggccaagg ccgacaccct ggcgaaggac ggcaaggtca ccgccgagcg
    10801 cgccgccgaa ttgaaggcgc tggcgcgcac cgcactgacc gacaacgtca agccggccta
    10861 cgacaaggtg atcgcattcg ccaccggcga cctgcccaac gccaaggtcg atcccgccgg
    10921 catcggcagc acccaggccg acggcgccgc gtattacacc taccagctca agcagaacac
    10981 caccacggcg atgagcgccg acgagatcca ccagttgggt ctgtccgaag tcgcgcgcct
    11041 gcgcggcgaa ctggagaaag tgaaggcaac ggtcgggttc gaaggcgacc tgccggcgtt
    11101 cttcaagcac gtgcagggcg acccgaacca gctgttcagc aacgacgacg ccggccgcaa
    11161 ggcgtacatc gacgaagcca cccgcgtcat cggcaacatc aaggaacacc tgccggacta
    11221 cttcggcctg ttgcccaagg cggacctggt ggtcaagcgg gtcgagccgt tccgcgagca
    11281 gcctggcgcc gcgcagcact actacccgag cacgccggat ggctcgcggc cgggcgtgta
    11341 ctacgcgcat ctctccgaca tgaaatcgat gccgaagacc acgcttgagg tcatcgcgta
    11401 ccacgagggc ctgcccggcc accacatgca gatcgccatc gcccaggaac tgaagggcgt
    11461 gccggtgttc cgcacccagt tcggcagcac cgcctatgcg gaaggctggg gcctgtacgc
    11521 ggaggcgctg gccaaggaaa tgcccggcac ctaccaggat ccgtattccg aatacggccg
    11581 gctgacctcg gaaatgtggc gcgcgatccg cctggtggtc gacaccggca tgcacgccaa
    11641 gggttggacc gagcagcagg cggtcgacta tttccgcgcg aacagctcga tccccgacgg
    11701 cgcgatcaag tcggaagtgc gccgctacct gatcatgccg ggccaggcca cggcctacaa
    11761 agtcggcatg atcaagatcc agcaactgcg caagaaggcg gaaaccgaac tcggcgaaca
    11821 gttcgacatc cgcggcttcc acgatgcggt gctgggcggt ggcgcgttgc cgctggacct
    11881 gctggagcgg cgcgtggaca gctggatcgc agggaagaag gcgggctgat
//
LOCUS       DAOOCX010000071        11869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5045_length_11869_cov_8.604198, whole genome shotgun sequence.
ACCESSION   DAOOCX010000071 DAOOCX010000000
VERSION     DAOOCX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11869
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5045_length_11869_cov_8.604198"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PK399_07915"
     CDS             <1..778
                     /locus_tag="PK399_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPW13082.1"
                     /translation="VRAMPLRTRWAYAGIGALVALHWLTFYGAIKLSNASVAATCIAF
                     ATPMTALVEPLLTRQRFQPRDLLLGVASLPGIWLVVGGVPAGMHAGIVAGVVSALFVA
                     LFGTLNKRMVDGGDALTVTALELGAGTLTLTLLAPVMPLLVPAFAGPLLVLPTGMDAF
                     WLVLLALACTLFPFALCLVALRHLSAFTAQLSVNLEPIYAIVLAAILFGEQQELGGKF
                     YAGVAIILGVVFAQGLLAARRKPTGHPEQAAVSEAHRIAD"
     gene            845..2458
                     /locus_tag="PK399_07920"
     CDS             845..2458
                     /locus_tag="PK399_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW13083.1"
                     /translation="MYLGYYGLHEAPFSITPDPRFVHLSERHRDALAHLLFGIDKGGG
                     GGFVQLTGEVGTGKTTLSRLLLEQLPENARIALVLNPRQNAIELLESICEELRIDITG
                     KRGSAKALVDALNVYLLDAYAQGLRVVLLIDEAQNLPADALEQVRLLTNLETDTQKLL
                     QILLLGQPELRAMVARPELRQLAQRITARFHLTPLDAKETAVYLRHRWRVAGGTRFPF
                     DAAAVQRLHARAGGVPRLINVIAERALLAGYARDATSIDAKLVDAAAGEVLPPVLARR
                     RWPLLAAAVGVIALALVAGWMWRSRDEVDTPARASAQATSHSPAASALPTPIVETETV
                     DAAGLVQLLGATGDTPLPAWQHLLGLWALPADAITVDPAGPCTPASRDDVHCVQGRAR
                     LDTLLALDRPILLRLQDGSARAWAVLLGADARSARVRVGARTVDIDRVLLQSRWNGDY
                     AGLWRGPAMLAKSPRLESTGPAVDWIRQRLSPTGNVVPGVPYDDALRASVRQLQDARG
                     LPADGIAGPLTLMALADDPPGPRLLRVLD"
     gene            2463..>2810
                     /locus_tag="PK399_07925"
     CDS             2463..>2810
                     /locus_tag="PK399_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13084.1"
                     /translation="MSLILEALRKSEAERRRGNTPDVAMELPPVPLRAHQSTPAWVWP
                     ALLAAIVVIAIAWWLAGRRDTDVPVASDTPAIATDPVPAAPQAPAVVPRTSTAAPVTT
                     PSTGIASAPASEAP"
     assembly_gap    2811..2910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2911..3117
                     /locus_tag="PK399_07930"
     CDS             <2911..3117
                     /locus_tag="PK399_07930"
                     /inference="COORDINATES: protein motif:HMM:NF027851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HPW13085.1"
                     /translation="APMPTAASMSGVKLSMHMWDASADKRFVILNGQRMGEGDRNGDV
                     QVIAIERDGVVVERDGQRARVALP"
     gene            complement(3121..3495)
                     /locus_tag="PK399_07935"
     CDS             complement(3121..3495)
                     /locus_tag="PK399_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW13086.1"
                     /translation="MLAYATLGTTDLPRAAAFYDSVLAPLGARRQMEEPDYFIAWGTT
                     EHGAGLGITYPFDKQAATVGNGCMVALGATSREQVDAVYAKAIELGGTDEGAPGERFP
                     GFYAAYFRDLDGNKLNCCCMGQ"
     gene            3558..3776
                     /locus_tag="PK399_07940"
     CDS             3558..3776
                     /locus_tag="PK399_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPW13087.1"
                     /translation="MQTLDFELDRDFIELNQLLKLAGLCDSGGAGKQLVASGVVRVDG
                     ATELRKTAKIRAGQQVQLDDVLIRVVAA"
     gene            3879..4379
                     /locus_tag="PK399_07945"
     CDS             3879..4379
                     /locus_tag="PK399_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW13088.1"
                     /translation="MTGHDHPQAFADVLQRHAGIVFKVANTYARLPEDRADLAQEIAA
                     QLWHAWPKYDPARSVSTWMYRVALNVAISHVRVQAVRQRHDMVPLDDGLHDLVDAAAP
                     DHESEQHLRLLQAFIARQPPLDRALLLLYLDERPQREIAEILGISEANVSTKVGRLKQ
                     RLRDEL"
     gene            4409..5068
                     /locus_tag="PK399_07950"
     CDS             4409..5068
                     /locus_tag="PK399_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13089.1"
                     /translation="MDTTMDLDDIKSAWQALDARLARQDALQLELLRGQKQAQARRNL
                     RPLLVGMLLQAVLGIGLVLLGVGCWRNNLEVPGLLAAGIALHAFGVLHIALAGIVSGL
                     ATTLDFAAPVLAIQKRLRLLLRIQVLNSNLCGAPWWVMWVLVVIGFAGLSPTPHVGPT
                     PTWIWISLALGIAGMVGTWWWAARGSRGPRDPLARMDDGADGIRRNLRAYDELERFER
                     E"
     gene            5205..6407
                     /locus_tag="PK399_07955"
     CDS             5205..6407
                     /locus_tag="PK399_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPW13090.1"
                     /translation="MHAALAHPPRERLHGLDAVRAIALLFGVVVHASMAWIPGAQYVW
                     VVHDGSPSEVAGLLFYVPHMFRMLLFFLIAGLFARMACERLGTIGFVRDRVRRIAIPL
                     ASLWFPMLMAIVAVIAWNAWLINNGTMPPAPPTPPLSPRNVPLTHLWFLYVLLLCYIA
                     ALALRATLGRSALLQRASDTCVRVLASPIGPLLLALPLAAALTTHPNWFAWFGIPTPD
                     QSLYPSLAAWVAFGSAFGFGWLLHRQQALLQRWTAWWPLHLAVAVLATIGCLLQVGLA
                     PKLVPATREIADIAYAIAYAVAAWSWTFAIVGLGLRFLSGHSPLRRYLADASYWIYIA
                     HLPLVMALQVVMSRVAWPWAAELALVLVATLAILLPSYDLLVRNTFVGSWLNGRRKPR
                     GLERIASR"
     gene            complement(6423..7676)
                     /locus_tag="PK399_07960"
     CDS             complement(6423..7676)
                     /locus_tag="PK399_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13091.1"
                     /translation="MRLPWILIVLCLLAGACSRQPEATLTSANANADAELDTGPVVQR
                     KPQDAIDWSPVQLEAGDAWVDCSADYTQGGGRPLLSLAYEDLRRSVQDCRGTGLLRLR
                     YKGKVTASFAALMERTGRVAGDLGIGKRILDIDSAGGHVEAAIRAGDAIGENDWTIWV
                     RGDSVCHSACVLLLAAGDMRVIAGPVGIHRMIRLRSSATSRKELGEELRTVSDELRNY
                     LERNGASDKVYDLMTTVPNRTLRILTVDELDQYGLSGANAVEDDLNRIRLVRRCGTDF
                     LQRRDAFRRAYDAQCAQAVDGQTQDVDQMNECGLALRGRFGFPGAECADESPMAELDG
                     AILMPRVSQDVPQEVAEPEASPAQPPAPAVRSGGQAVSSGRNPPETDAAPAQHGPSAS
                     EEPAPEAAEESATAEPQDALPELAR"
     gene            7814..8953
                     /locus_tag="PK399_07965"
     CDS             7814..8953
                     /locus_tag="PK399_07965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HPW13092.1"
                     /translation="MSALPVPTARRSWLAHISAALGLSALLGVLCFHFPELLTSRDFR
                     AVYTEVFARHLLLFGLIAAFTTGTFAILRGRNRRVAMLGVGSAAVAVVLGGATVHFDA
                     IDSTPFSLGLDWFVISLFFSALVFIPIERMAPVRPMSPLRGEWRTDLAYFFMGHVLVQ
                     FILIAVTASTGTIAALAAFPSVKALIQSLPVWAQFLLAVLVADLAQALLHRAYHNIPF
                     LWRFHAVHHSTRRMDWLAGSRIHLLEIILTRSAVLLPLVVLGFAPAAVNAYVILVGLQ
                     AVLAHANLRLDFGWLEYVLVTPRYHHWHHARQAEYVDVNYAIHLPLVDMLMGTFRRPP
                     RGEWPAEYGVMKLETVPVGFLAQARMPLSRRKAWEDYVGRDADAG"
     gene            complement(8923..10122)
                     /locus_tag="PK399_07970"
     CDS             complement(8923..10122)
                     /locus_tag="PK399_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HPW13093.1"
                     /translation="MKLLYAPDTADHPNWGCRFMGDWYRQALVDLDGVRLRRLGSRWF
                     FRDDPHVPAPRTWAELLVVAHAVREGRSQGLSAVLPMLAACDLLFINGENFIRPGTLK
                     GRRLLMLAYLAKAAFGKAVVLTNLTLDLSEPALAELVGNVLPLLDEVHVREDVSAASY
                     RAQVEQARFLQFADVGWTATPAPIEAWRALARRRGHFHAWPDHCAAFDPAQPYVTVSA
                     SSAFVGTAQGEDPAEAFVALCRQLAQQVAQVVLVAPCEVDAMIMRKVQAATGFPLLGL
                     NLPVAQGIDVLGNARVHVGGRWHPGIFAATGGTPLVGMGANTHKMATLMQQLHPDEPV
                     FEARSLARHVDAIVSRAKAQVDAGSALRAQVLERAAAMRARVEGNLGFVERRLVEAQP
                     ASASRPT"
     gene            complement(10179..10655)
                     /locus_tag="PK399_07975"
     CDS             complement(10179..10655)
                     /locus_tag="PK399_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004426258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13094.1"
                     /translation="MAAKHGVLWLVVLLAITGVALAQRAYVGIEQRLSAAQLQEIGLT
                     PTQVALLDRYLAEAAASAPSEEHAPVERREADPTRFAGLDAERIAGKVQGMVAGWEPG
                     TIFKLDNGQQWQVLKGSMKLRKPLQDPQVLLVPGIAGRWFLQVDEDMPKARVFRID"
     gene            complement(10718..11398)
                     /locus_tag="PK399_07980"
     CDS             complement(10718..11398)
                     /locus_tag="PK399_07980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW13095.1"
                     /translation="MTAHPLDNPFFSALESLHAGIALRAGEVLRYPMDVAPFLGIPHP
                     GVDLADPLADLVPQGDSVLLLGIAPTAVPHGWALERFADLAQMQCDRELPLIDGPGIV
                     ELGDADRADVLALTALVYPHYFRPRTMDLGRYFGIYVEGRLAAMIGERLGAPGFREMS
                     AICTHPDFLGRGYARRLTAFLTNQTLQDGRQPFLHVAYANDRARMLYEQMGYRLRRDI
                     PFLALRRG"
     gene            11511..11867
                     /locus_tag="PK399_07985"
     CDS             11511..11867
                     /locus_tag="PK399_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HPW13096.1"
                     /translation="MSYIDGFVIAAPTASRQEVIDYAARFDPMFLELGALRVVECWGV
                     DVPHGTVTDFHRAVQAKDDETVMFSWIEWPDKATRDAGMQKMMEDPRMEAANPMPFDG
                     KRMIFGSFENVLDLRA"
BASE COUNT         1725 a         4148 c         3975 g         1921 t
ORIGIN      
        1 ggtgcgggcg atgccgctgc gcacccgctg ggcgtatgcg gggatcggcg cgctggtcgc
       61 gttgcactgg ctgaccttct acggcgcgat caagttgtcg aatgcttcgg tggcggcaac
      121 ctgcatcgcc ttcgccacgc cgatgacggc gttggtcgag ccgctgctga cccggcaacg
      181 cttccagccg cgcgacctgc tgctcggcgt ggcctcgctg ccggggatct ggctggtagt
      241 cggcggggtg cccgcgggca tgcatgccgg gatcgtggcc ggggtggtct ccgcgctgtt
      301 cgtcgccctg ttcggcacgc tcaacaagcg catggtcgat ggcggcgatg cgctgaccgt
      361 caccgcactg gaactcggcg cgggcacatt gacgctgacc ctgctggcac cggtgatgcc
      421 gctgctggtg cccgccttcg ccggcccgtt gctggtgctg cccaccggga tggatgcatt
      481 ctggctggtg ctgctggcgc tggcctgcac gctgttcccg ttcgcgctgt gcctggtcgc
      541 gctccgccac ctgtccgcct tcaccgcgca gctgtcggtg aacctggagc cgatctacgc
      601 gatcgtgctg gccgcgatcc tgttcggcga gcagcaggaa ctcggcggca agttctacgc
      661 cggcgtggcg atcatcctcg gcgtcgtctt cgcgcagggc ctgctggcgg cacggcgcaa
      721 gccgaccggg catccggagc aggccgcggt cagcgaggcc catcggatcg ccgactgaac
      781 cgtgcggttc gcgtcgcggc gcgcgccgtg gcaggctagt cgcttccccg tcgacgccca
      841 tcgcatgtat ctcggctact acggcctgca cgaagcgccg ttctcgatca ccccggatcc
      901 gcgcttcgtc cacctcagcg agcgccaccg cgatgccttg gcgcacctgc tgttcggcat
      961 cgacaagggc ggcggcggtg gcttcgtcca gctcaccggg gaagtcggca ccggcaagac
     1021 cacgctgagc cggctgctgc tggagcaact gcccgagaac gctcggatcg cgctggtgct
     1081 gaacccgcgg cagaacgcga tcgagttgct ggagtcaatc tgcgaagaac tccgcatcga
     1141 catcaccggc aagcgcggtt cggcgaaggc gctggtcgat gcgctcaacg tctacctgct
     1201 cgatgcctac gcgcagggtt tgcgcgtggt cctgctgatc gacgaggcgc agaacctgcc
     1261 ggccgatgcg ctggagcagg tgcgcctgct caccaacctg gaaaccgaca cccagaagct
     1321 gctgcagatc ctgctgctgg gccagccgga actgcgcgcg atggtggccc ggccggagct
     1381 gcgccagctc gcgcaacgca tcaccgcgcg cttccacctg acccctctcg acgccaagga
     1441 aaccgcggtc tacctgcgcc accgctggcg cgtggccggc ggcacccgct tcccgttcga
     1501 cgccgccgcg gtgcaacgcc tgcatgcacg tgccggcggc gtaccgcgcc tgatcaacgt
     1561 gatcgccgaa cgcgcgctgc tggccggcta cgctcgcgac gccaccagca tcgacgcgaa
     1621 gctggtcgat gccgcggccg gggaagtgtt gccgccggtg ctggcccgtc gccgttggcc
     1681 gctcctggct gcggcggtcg gggtcatcgc gctcgcgctg gtcgcgggct ggatgtggcg
     1741 cagtcgcgac gaggtcgaca cgccagcgcg ggcttcggca caagcgacgt cgcactcgcc
     1801 cgcagcgagc gccttgccca cgccgatcgt cgaaaccgaa accgttgatg ccgcgggact
     1861 cgtccaactg ctcggcgcga ccggcgacac gccgttgccg gcctggcaac acctgctcgg
     1921 cctgtgggcc ttgcccgcgg atgcgatcac cgtcgatccg gcggggccgt gcacgccggc
     1981 gtcgcgggac gacgtgcatt gcgtgcaggg ccgtgcacgc ctggacaccc tgcttgccct
     2041 cgaccgcccg atcctgctgc gcctgcagga cggcagcgcg cgagcgtggg cggtcctgct
     2101 cggcgcggac gcgcgcagcg cgcgggtgcg ggtcggtgca cgcacggtcg acatcgaccg
     2161 cgtgttgctg cagtcgcgct ggaacggcga ctacgccggc ctgtggcgcg gtccggcgat
     2221 gctggccaag tcaccgcggc tggaaagcac cggacccgcg gtcgactgga tccgccagcg
     2281 cctgtcgccc accggcaatg tcgtgcccgg cgtgccgtac gacgatgcgc tgcgcgcctc
     2341 ggtgcgccag ttgcaggacg cgcgcggcct gcccgcggac ggcatcgccg gaccgctcac
     2401 cctgatggcg ttggccgacg atccacccgg tccgcgcctg ctccgcgtcc tggactgacc
     2461 cgatgtcgct gatcctcgaa gccctgcgca agtcggaagc cgaacgccgt cgcggcaaca
     2521 cgcccgacgt ggcgatggag ctcccgccag tgccattgcg cgcccatcaa tccacgccag
     2581 cgtgggtgtg gcccgcgctg cttgccgcca tcgtcgtcat cgcgatcgcg tggtggctgg
     2641 ctggacgccg cgataccgac gtaccggtcg catcggacac acccgccatt gccaccgatc
     2701 cggtgccggc cgcgccgcag gcacccgcgg tcgtcccgcg cacctccacc gccgcaccgg
     2761 tcacgacacc ttcgacgggc atcgcatccg cgcccgccag cgaagcgcca nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccccgatgc ccacggcggc atcgatgagc
     2941 ggggtgaaac tcagcatgca catgtgggac gcgagtgcgg acaagcgctt cgtcatcctc
     3001 aacggccagc gcatgggcga aggcgaccgc aacggcgacg tgcaggtcat cgccatcgaa
     3061 cgcgacggcg tggtggtgga gcgcgacgga cagcgcgcgc gggtcgcgct gccctgaggc
     3121 ttactggccc atgcagcagc agttgagctt gttgccgtcc aggtcgcgga agtaggcggc
     3181 atagaagccg gggaagcgct cgccgggcgc gccctcgtcg gtaccgccga gctcgatggc
     3241 cttcgcgtag accgcatcga cctgttcgcg cgacgtcgca cccagcgcca ccatgcagcc
     3301 attgcccacg gtggccgcct gcttgtcgaa cggataggtg atgcccaggc cggcgccgtg
     3361 ctcggttgtg ccccaggcga tgaagtaatc cggctcttcc atctgccgcc tggcgccgag
     3421 cggggcgagc acgctgtcgt agaacgcggc ggcgcgcggc aggtcggtgg taccgagggt
     3481 ggcgtaggca agcatggcga ggctccgaca ggaagggagc gcagcctaca cccggcggcg
     3541 ggcgctatgc tggccacatg cagaccctcg acttcgaact ggaccgcgac ttcatcgaac
     3601 tcaaccagct gttgaagctg gctggcctct gcgacagcgg cggtgccggc aagcagctgg
     3661 tcgccagcgg cgtggtgcgg gtggatggcg ccaccgagct gcgcaagacc gcgaagatcc
     3721 gcgctggcca gcaggtccag ctcgacgacg tgctgatccg ggtcgtggcc gcctgatctt
     3781 tttcgcggtt ccggtgtgag atcccccacg ctggcgcgaa tcatggtctc gaagggcgcg
     3841 atggacgcgc cggggacgcc atggagacca gcgccgccat gaccgggcac gatcatccgc
     3901 aggcctttgc cgatgtgctg caacggcatg ccgggatcgt gttcaaggtc gccaatacct
     3961 atgcacgcct gccggaagat cgtgccgacc tggcgcagga gatcgccgcg caactctggc
     4021 atgcgtggcc gaaatacgat cccgcgcgca gtgtcagcac ctggatgtac cgggtcgcgc
     4081 tgaacgtggc gatcagccat gtacgcgtgc aggcggtgcg ccagcgccac gacatggtgc
     4141 cgctggacga cggcctgcac gacctggtcg atgccgcggc gcccgaccac gaatccgagc
     4201 aacacctgcg cctgctgcag gccttcatcg cccgccagcc gccgctggat cgcgccctct
     4261 tgctgctcta cctggatgaa cgcccgcaac gcgagatcgc cgagatcctg ggcatcagcg
     4321 aagccaatgt ctccacaaag gtcgggcgcc tcaagcaacg cctgcgcgac gaactctgag
     4381 ccgaaccggc atcgaaccga ctacgggaat ggacacgacc atggacctcg acgacatcaa
     4441 atccgcctgg caggcgctgg acgcgcgcct ggcccgccag gacgcactgc aactggaact
     4501 cctgcgtggc cagaagcagg cgcaggcgcg ccgcaacctg cgcccgctgc tggtcggcat
     4561 gctgctgcag gcggtgctcg gtatcggcct ggtgctgctg ggcgtgggct gctggcgcaa
     4621 caacctggag gtgcccggcc tgctcgccgc cggcatcgcc ctgcatgcct tcggcgtcct
     4681 gcacatcgcc ctggcgggga tcgtcagcgg gctggcgacc acgctcgact tcgccgcgcc
     4741 ggtactcgcc atccagaagc gcctgcgcct gctgctgcgg atccaggtcc tgaattccaa
     4801 cctgtgcggt gcgccctggt gggtgatgtg ggtgctggtg gtgatcggct tcgcggggtt
     4861 gtcgcccacg ccgcacgtcg gcccgacccc gacctggatc tggatcagcc tggccctcgg
     4921 catcgccgga atggtcggca cctggtggtg ggcggcacgc ggctcgcgtg gcccgcgcga
     4981 tccactggcg cgcatggacg atggcgccga tggcatccgc cgcaacctgc gcgcctacga
     5041 cgaactggaa cgcttcgagc gcgagtgatg cgagaccacg cgcgccactg atccactgcg
     5101 cgccctgagc cggcacagcc ggctcccgag caaatcaacg acaccagccg caagcccagc
     5161 gcttgcggca cggccctgca catcccgaat ccggagaacc cccaatgcac gctgccctcg
     5221 cccacccacc ccgagaacgc ctgcacgggc tggatgcggt ccgcgccatc gccctgctgt
     5281 tcggcgtggt cgtgcatgcc agcatggcct ggattcccgg cgcgcagtac gtctgggtcg
     5341 tccacgatgg ctcaccgagc gaggtggcgg gcctgctgtt ctacgtgccg cacatgttcc
     5401 gcatgctgct gttcttcctg atcgccggct tgttcgcgcg gatggcctgc gagcgacttg
     5461 gcaccatcgg cttcgtgcgc gaccgcgtgc gccgcatcgc catcccgctg gcctcgctgt
     5521 ggttcccgat gctgatggcg atcgtcgcgg tgatcgcctg gaatgcctgg ctgatcaaca
     5581 acggcaccat gccgcctgcg ccgccaacgc cgccgctgtc gccgcgcaat gtcccgctga
     5641 cccatctgtg gttcctgtac gtgttgctgc tctgttacat cgccgcgctc gcgctgcgcg
     5701 caacgctcgg tcgcagtgcg ttgctgcaac gcgcgagtga tacctgcgtg cgcgtgctcg
     5761 catcgcccat cggaccgttg ttgctggcgc tgcccttggc cgccgcgctg accacccatc
     5821 cgaattggtt cgcgtggttc ggcatcccca cgcccgacca gtcgctgtat cccagcctgg
     5881 ccgcatgggt cgccttcggc agcgcgttcg ggttcggctg gttgctgcat cggcaacagg
     5941 ccctgctgca gcgttggacc gcgtggtggc cgttgcatct ggctgtcgcg gtgctggcca
     6001 ccatcgggtg cctgctgcag gtcgggcttg caccgaagct cgtaccggcg acacgcgaga
     6061 tcgccgacat cgcctatgcg atcgcctacg ccgtcgcggc ctggagctgg accttcgcca
     6121 tcgtcggcct gggcctgcgc ttcctgtccg ggcacagccc gctgcgccgc tacctggccg
     6181 atgcgtcgta ctggatctac atcgcccacc tgccgctggt catggccttg caggtcgtga
     6241 tgtcgcgggt ggcgtggccg tgggccgcgg agttggcgct ggtgctggtc gcgacgctcg
     6301 cgatcctgct gcccagctac gacctgctgg tgcgcaacac cttcgtcggt agctggctca
     6361 acggccggcg caagccgcgc gggctggagc ggatcgcatc gcgctgagcg cggatcgccg
     6421 actcaacggg cgagttcggg caaggcgtcc tgcggctccg cggtagccga ttcctcggcg
     6481 gcctccggag caggttcttc gctggcggat ggcccgtgtt gtgccggcgc ggcatcggtc
     6541 tcaggcgggt tccgcccgga cgagaccgcc tggccgcccg aacgcaccgc cggcgcaggc
     6601 ggctgcgcgg gcgacgcttc cggctccgcc acctcctgcg gcacgtcttg cgacacacgc
     6661 ggcatcagga tcgcgccatc cagctcggcc atcgggcttt cgtccgcgca ttccgcaccg
     6721 gggaaaccga aacgaccacg cagggccagg ccgcattcgt tcatctgatc gacgtcctgg
     6781 gtctggccat cgaccgcctg cgcgcactgg gcatcgtagg cgcggcggaa cgcatcgcgg
     6841 cgctgcagga aatcggtgcc gcagcggcgc accaggcgaa tccggttgag gtcgtcctcc
     6901 accgcgttgg ccccggacag gccgtactgg tcgagttcgt ccacggtgag gatgcgcagg
     6961 gtgcggttcg gcaccgtggt catcaggtcg tagaccttgt cggaggcgcc gttgcgctcc
     7021 aggtagttgc gcagttcgtc gctgaccgtg cgcaattctt cgccgagttc cttgcgcgag
     7081 gtcgcagacg agcgcaggcg gatcatccgg tggatgccca ccgggccggc gatgacgcgc
     7141 atgtcgcccg ccgccagcag tagcacgcag gcgctatggc agaccgaatc gccgcgcacc
     7201 cagatcgtcc agtcgttctc gccgatggcg tcgccggcgc gaatcgcggc ttccacatgg
     7261 ccgccggcgg aatcgatgtc gaggatgcgc ttgccgatgc cgaggtcgcc cgccacccgg
     7321 ccggtgcgct ccatcagcgc ggcgaagctg gcggtgacct tgcccttgta gcgcagccgc
     7381 aacagcccgg taccacggca atcctgcacg ctgcggcgca ggtcctcata cgccagcgac
     7441 agcaggggcc ggccaccacc ctgggtgtag tccgcgctgc aatcgaccca ggcgtcgccg
     7501 gcctccagct gcaccggcga ccagtcgatc gcatcctgcg gcttgcgctg caccaccggc
     7561 ccggtgtcca gctccgcgtc cgcgttcgcg ttcgcactgg tcagggtggc ctcgggttgg
     7621 cgactgcagg caccggccag caggcaaagc acgatcagga tccaggggag gcgcatcgaa
     7681 ccgtgtgggc ggcggggacg cgtcagtcta ggccgcattc ctgccggtgc gcggaaccgg
     7741 tcgtgaaccg tggacgccgt agcccgggcg cccggcggtt gtccctggtg caggcggccg
     7801 ctaccgtacg gcgatgagcg cactccccgt cccgaccgcg cgccggtcct ggctggccca
     7861 catctccgcg gcccttggcc tgtcggccct gctcggcgtg ctctgcttcc atttcccgga
     7921 actgctgacc agccgcgatt tccgcgcggt ctataccgaa gtcttcgccc gccacctgct
     7981 gctgttcgga ctcatcgcgg cgttcaccac cggcacgttc gcgatcctgc gcgggcgcaa
     8041 ccggcgggtg gcgatgctcg gagtcggcag cgcggccgtg gcagtggtgc tgggcggcgc
     8101 caccgtgcat ttcgacgcga tcgattccac gccgttctcg cttggactgg actggttcgt
     8161 gatctcgctg ttcttctccg cgctggtctt catccccatc gaacggatgg cgccggtgcg
     8221 gccgatgtcg ccgctgcgcg gggagtggcg gaccgacctg gcctacttct tcatgggcca
     8281 tgtgctggtg cagttcatcc tgatcgcggt gaccgcatcc accggcacga tcgccgcgct
     8341 tgccgcgttc ccgtcggtca aggccctgat ccagtcgctg ccggtgtggg cgcagttcct
     8401 gctggcggtg ttggtggctg acctggccca ggccttgctg catcgcgcgt accacaacat
     8461 cccgttcctc tggcgcttcc atgccgtgca ccactcgacc cggcggatgg actggcttgc
     8521 gggttcgcgc atccacctgc tggaaatcat cctgacccgc agcgcggtgc tgctgccgct
     8581 ggtggtgctc ggtttcgcgc cggcggcggt gaatgcctat gtgatcctgg tcggcctgca
     8641 ggcggtgctg gcgcatgcca acctgcggct ggatttcggc tggctggaat acgtgctggt
     8701 cacgccgcgc taccaccact ggcaccacgc gcggcaagcc gagtacgtcg acgtgaacta
     8761 cgccatccac ctgccgctgg tcgacatgct gatgggcacg ttcaggcgac cgccgcgcgg
     8821 cgaatggcca gccgaatacg gggtgatgaa actcgagacc gtgccggtcg gattcctggc
     8881 gcaggcgcgg atgccgctgt cgcgcaggaa ggcctgggag gactacgtcg ggcgcgatgc
     8941 cgacgccggc tgagcctcca cgagccgtct ttcgacgaaa cccaggttgc cctcgacccg
     9001 cgcgcgcatc gccgcggcgc gctccagcac ctgagcgcgc agggctgatc ctgcatcgac
     9061 ctgcgccttg gcgcgggaca cgatggcgtc gacatggcgc gccaggctgc gcgcctcgaa
     9121 gaccggttcg tcgggatgca actgctgcat cagcgttgcc atcttgtgcg tgttggcgcc
     9181 catccccacc agtggcgtgc cgccggtcgc ggcgaagatg ccggggtgcc agcgcccgcc
     9241 cacgtgcacg cgcgcgttgc cgagcacgtc gatgccctgc gcgaccggca ggttcaagcc
     9301 aagcaagggg aaaccggtgg cggcctgcac cttgcgcatg atcatggcgt cgacttcgca
     9361 cggcgccacc agcaccacct gcgcgacctg ttgcgccaat tgccggcaca acgccacgaa
     9421 agcctcggcc ggatcctcgc cctgcgcggt gccgacgaac gcggagcttg cgctcaccgt
     9481 cacatagggt tgcgcgggat cgaatgcggc gcagtggtcg ggccatgcgt ggaaatggcc
     9541 gcggcgccgg gccagcgcgc gccatgcctc gatcggtgcc ggcgtggcgg tccagcccac
     9601 gtcggcgaat tgcaggaagc gcgcctgctc cacctgcgcg cggtacgacg cggcggagac
     9661 atcctcgcgg acatgcacct catcgagcag cggcagcaca ttccccacca gttcggccag
     9721 cgcgggttcc gacaggtcga gggtgaggtt ggtcagcacc acggccttgc cgaacgccgc
     9781 cttcgccaga taggccagca tcagcaggcg tcggcctttc agcgtgccgg ggcggatgaa
     9841 gttctcgcca ttgatgaaga gcaggtcgca ggcggccagc atcggcagga ctgcggatag
     9901 tccctggctg cgaccttcgc gcacagcgtg cgcgacgacg agcagctccg cccacgtcct
     9961 tggcgcaggc acgtggggat cgtcgcggaa gaaccagcgg cttcccaggc ggcgcaggcg
    10021 cacgccatcg aggtcgacca gcgcctgccg ataccagtcc cccatgaaac ggcaacccca
    10081 gttcggatgg tcggcggtgt cgggggcgta gagtagtttc atgcgcacga ccttggcgca
    10141 aatgccgcga ccgcacaatc accacgactc cggcgcaact agtcgatcct gaacaccctc
    10201 gccttgggca tgtcctcgtc gacctgcagg aaccagcgtc cggcgatccc gggcaccagc
    10261 agcacctgcg ggtcctgcag cggcttgcgc agtttcatgc tgcctttcag gacctgccat
    10321 tgctggccat tgtcgagctt gaagatggtg cccggctccc agccggcgac cattccctgc
    10381 accttgccgg cgatgcgttc ggcatccagg ccggcgaacc gggtcgggtc cgcctcgcga
    10441 cgctcgacgg gcgcatgttc ctccgagggt gcgcttgcag cggcttcggc caggtagcga
    10501 tccagcagcg ccacttgcgt cggggtcagc ccgatttcct gcaattgcgc ggcactcagg
    10561 cgctgctcga tgccgacata ggcacgttgc gccagcgcga cgccggtgat cgcaagcaac
    10621 accaccaacc acaagactcc gtgcttcgcc gccatgcctg ccacgctcct cgtcggatgc
    10681 gacaagtctg cgacgcctgc atgacagttt cgcgacgtca gccgcgccgc agggcgagga
    10741 acgggatgtc gcggcgcagg cggtagccca tctgctcgta cagcatccgt gcgcggtcgt
    10801 tggcgtatgc cacgtgcagg aacggttgcc tgccgtcctg cagcgtctga ttggtgagga
    10861 aggccgtcag acgccgtgcg tagccgcgcc cgaggaaatc cggatgcgtg cagatcgcgc
    10921 tcatctcgcg gaacccgggt gcgcccaggc gctcgccgat catcgccgcc aaccggcctt
    10981 ccacgtagat gccgaaatag cgcccgagat ccatcgtgcg cggacggaaa taatgcggat
    11041 acaccagcgc ggtcagtgcg agcacgtctg cgcgatcggc atcgccaagt tcgacgatgc
    11101 ctgggccgtc gatgagcggc agttcgcggt cgcactgcat ctgcgccagg tcggcgaacc
    11161 gctcaagcgc ccaaccgtgc ggaaccgccg ttggcgcgat gccgagcaac aacacgctgt
    11221 cgccttgcgg caccaggtcc gccaggggat cggccaggtc gacaccgggg tgcgggatgc
    11281 ccaggaacgg cgcgacgtcc atcggatagc gcagcacctc gcccgcccgc agcgcgatgc
    11341 ccgcatgcag gctctccagc gccgagaaga acgggttgtc gagcggatgc gccgtcatcc
    11401 cggcagtgtg ccgatcacgg catctttccg ccacgctgtc gaaactggcg gcccgcattc
    11461 gtcatggtca ttgcagcatc catccacgcc cgccaccaac ggagaacgcc atgagctaca
    11521 tcgacggttt cgtgatcgcc gcccccaccg ccagccggca ggaggtcatc gactatgccg
    11581 cccgcttcga cccgatgttc ctggaactgg gcgcgttgcg cgtggtggag tgctggggcg
    11641 tcgacgtgcc gcacggcacc gtcaccgact tccaccgcgc ggtgcaggcg aaagacgatg
    11701 aaacggtgat gttttcgtgg atcgaatggc cggacaaggc gacccgcgac gccggcatgc
    11761 agaagatgat ggaagacccg cggatggagg cggccaaccc gatgcccttc gacggcaagc
    11821 ggatgatctt cggcagcttc gagaacgtgc tcgacctcag ggcctgagc
//
LOCUS       DAOOCX010000072        11702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5138_length_11702_cov_8.718812, whole genome shotgun sequence.
ACCESSION   DAOOCX010000072 DAOOCX010000000
VERSION     DAOOCX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 11702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11702
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5138_length_11702_cov_8.718812"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PK399_07990"
     CDS             complement(<1..344)
                     /locus_tag="PK399_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HPW13097.1"
                     /translation="MPASLLEFLSGGLLQFGWWAMLAYLLVVTQITIFTVTLYLHRSQ
                     AHRGVDFHPVIAHFFRFWSWLTTSMITREWAAIHRKHHAKCETEEDPHSPMHKGIGNV
                     LWKGADMYRESRL"
     gene            483..1310
                     /gene="mutM"
                     /locus_tag="PK399_07995"
     CDS             483..1310
                     /gene="mutM"
                     /locus_tag="PK399_07995"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPW13098.1"
                     /translation="MPELPEVETTRRGLLPHVAGRHVDHVLLRRPDLRWPIPREVENL
                     LPGQRIDAIRRRAKYLLLDTEAGSALLHLGMSGSLRVLPSDTPVRAHDHVDIALVCAD
                     GMPDRVLRFNDPRRFGCLLWQAPGTVHPLLADLGPEPLSDDFDGDVLFALSRGRSAPV
                     KSFLMDQAVVVGVGNIYAAEALFMAGISPLRAAGKVSRERYAALADAVKAILAHAIAR
                     GGTTLRDFLSPDGAPGYFEQELTVYGRGGEPCRRCGRPLKQALIGQRASAWCGHCQR"
     gene            1375..1953
                     /locus_tag="PK399_08000"
     CDS             1375..1953
                     /locus_tag="PK399_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HPW13099.1"
                     /translation="MTEAGEITVLLDAARDGDRGALDRVLATLYQELHTMARRQLAGQ
                     QGHTLDATSLVHEAYLKLIGRGTGAAQFDDRAHFFAYAASAMRSVVVDYARQRLAQKR
                     GGDLHRVTDLPEDIEGGIRLDEDMLGLDTALTRLTAVDPRLTHVVELRYFAGLSELEI
                     AALLKRSERSVRRDWQKARMFLLASLRDTPAA"
     gene            1977..4775
                     /locus_tag="PK399_08005"
     CDS             1977..4775
                     /locus_tag="PK399_08005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPW13100.1"
                     /translation="MDAERWQRLSPLLDALLELAPEQRSEHLAALRREDPVLADELAG
                     LLAMEADDEDFLSEPVVELPSGARPGARIGPYRLDRLIGEGGMGQVWLSVRADGLYER
                     KVALKLLRPGLADPRLRQRFDREREILARFAHPHIARLLDAGIDKDGQPYLALEYVEG
                     EPLTDYCRTRQLDIAARLDLFRQVCEAVSHAHANLIVHRDLKPSNILVTPAGHVRLLD
                     FGIAKLLDVDPLPVEQTRTGVRTFTLHYAAPEQIRGEPVTTMTDVYSLGVVLYELLTG
                     SKPYRLKRQTDAEWEEAILDGEPLKPSQVAARAGEACIRPYTPQRLARALSGDLDNIV
                     LKALRKRPEQRYVSAEALSQDLLCYLRGRPVSARGEGMAYRSRKYLHRHRWTIAAATT
                     VLAVLFTALGVVGWQAQRAMREASRAQAMQGFVADLFESAGTSSASEPIDLRKLLDMG
                     IVRGERSLVRQPQARAELYGVVARLRLGLGDYREANTLLDKQEVLLANLPDAPVSLRL
                     EAATLRGNAYRQLGNLPGCLQVMQRMEDTARREEERLPTQAAAFYSQQGRCHQELDQS
                     TLANRLYMRSLQLRRKQGDDVGVAENLLDIAKMHAADGAPEVAVPILADALAELRRKV
                     GDRHPLVIDMLRSLCALDRATDRLDDAVRHCRESQALAEDLHGRDHRASIDANRQLAA
                     LYVDLGRFSQAESIFLQTFGWMRARLDRNHPDLARAYNSLAIVAWERGDIERALDFQR
                     QAVAGWKTGHNDGLTANGLFNLAMILHSVGRDREALPAVRESRALRAQQFGDTHELVG
                     DSDHLLGEVLAGLGEPDAARAALQQAVRLTLAGYGPEHSHTRRAEISLARLRANEGAP
                     DALLQLTAYGQSGSGDIEQRKAAWLARAYAAEVECRTGSPTARASLDTVLTEMQLVLP
                     EGGAIPREVAAIRKACVR"
     gene            complement(4881..5501)
                     /locus_tag="PK399_08010"
     CDS             complement(4881..5501)
                     /locus_tag="PK399_08010"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPW13101.1"
                     /translation="MAKLYFYFSAMNAGKTTTLLQSAHNYRERGMRVAILTPKLDFRA
                     GSGTVASRIGLQAEGIAFERTDDLRHIIDADIAAHGPLHCVLVDEAQFLSKAQAWQLS
                     EVVDALRIPVLCYGLRTDFRGELFEGSQYLLAWADELQEIKTICHSGKKATMNVRIDA
                     QGRALQDGPQVEIGGNERYVSVSRAEFKKVMRGEGTIEPLQAPLPL"
     gene            5578..7572
                     /locus_tag="PK399_08015"
     CDS             5578..7572
                     /locus_tag="PK399_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPW13102.1"
                     /translation="MNGLNPPQRQAVLHTEGPLLVLAGAGSGKTRVIIEKIAHLIHSG
                     RYPAKRIAAITFTNKSAREMRERVAKRIRGDAAQELTVSTFHALGLRILQIDHAKLGL
                     RRGFSIFDADDSASQVKDLLGPGTKPDAIDAMKQLISRAKNAGLSPEQAAAAALTGRE
                     REAAALYARYQQRLAAFNAVDFDDLIRLPVQLLETDADAVAGWRERIGYLLVDECQDT
                     NDAQYRLLKAIAGDAGQFTCVGDDDQSIYAWRGANPDNLLALGTDYPALKIVKLEQNY
                     RCSNRVLRAANAVIANNPHEHPKTLWSDAADGERIRVWECRDAAHEADKVAGEIQFVA
                     QKHTPPNGVPPWSEFCILFRGNHQSRALEKSLQLLRIPYHLSGGTAFLDRGEVKDALA
                     WLRLLANPDDDAAFLRAVQSPNRGVGATSLQRLGELANHAHLPLSRAADSITLAKQLP
                     ARAANALQGFSDIVRGLRIDARTLPPAELVRKLNERSGLLNALRVLCKDETQFQARRR
                     NLDELADWFDGPKTSGPGELAAALALLSHADKGDAGNQVRLMSMHAAKGLEFRFVFIV
                     GVEDGNLPHEASIEEGRIDEERRLFYVGITRAKEQLWLSHAKEAQRWGDKLRLKPSRF
                     IDELPAAELQRDGADPVADAAHKAERRSAGFANIRALLGE"
     gene            7649..8563
                     /locus_tag="PK399_08020"
     CDS             7649..8563
                     /locus_tag="PK399_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HPW13103.1"
                     /translation="MRPAVAVSLLAAALSLAACKPTSLTAPSGAAAKPATATAAKPAT
                     GDVGANDNLNAVLWVQRAAEYEAVTQTVYRAAADQLDAALKQPNWDALVPGERGNAAT
                     GLPPAVVMDVDETVLDNSPYQARLVHDGASYDEATWDLWVAEKKAKPVPGVVDFAKAA
                     AAKGVTILYISNRAVHLKDATIANLRAEGLPVKDDSVFLGLGTFVEGCEQNGSEKLCR
                     RKLAGQKYRVLMQFGDQLGDFAEIVSNTPKDRDALYAEYADWFGERWYMLPNPTYGSW
                     EPAVFNNAWEQPAADRRKAKLDALELAK"
     gene            8786..9736
                     /locus_tag="PK399_08025"
     CDS             8786..9736
                     /locus_tag="PK399_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13104.1"
                     /translation="MTQRFVGRRLHFALAAALCLTLPTACERGGVTTGQTIGVARVAR
                     VEAATRPIDAAYVLRDRLLAHDGAGFARLAVPADLHARLATGWKDGRSRWPLDELPLD
                     AKLPGMLATLQAPGADKALMATFRRQFAGADRDIDQAIRTLALFGGEYVQNDGDYTPE
                     EREHIGQAIIALQAWGLQAPFADKARAQRFFTALTAAARRSGIEGKAGTAAFGKLGMR
                     ASLTRLSPFLATLLAQLRQQYGLDVDASLRALKVTLLQQTGDTARLRLQYPLAGRDID
                     AVVPAVRIDGHWYLADFVQRADASLAAKPAVAGGRKASPP"
     gene            9745..>11702
                     /gene="plsB"
                     /locus_tag="PK399_08030"
     CDS             9745..>11702
                     /gene="plsB"
                     /locus_tag="PK399_08030"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HPW13105.1"
                     /translation="MQSRPPQPSQASLFDAPPAPETPSEGAAPATDGADGVPAVEADP
                     LPSLPLPGFLAVPDPAPAEADAAVGVGAGIDPVTPSLDIDPAPPADAAEHAAPEAAPA
                     PPPMRPAKAKRPLWARILGRIVDPWLKLDIEPGDPDQFRQFDDGRPVCYVLEDYGLSN
                     ALILERACREAGLPSPLQPIPGDPLGRKRSYVALSRRHANALLSLGLVPGPEHKTHSG
                     SLARLLEAHRANAALDVQLVPVSIFVGQAPNRSSGWFSVLFSENWAIVGRFRRLLAIL
                     LNGRDTLVQFAPPVSLRGIVAEDLPPERTVRKLSRVLRTHFRRLREVVVGPDLSTRRM
                     LVDKVLAAEPVREAIADQAKRDKTTADAAWQKAHAYAYEIAADYSHTVVRSASFLLTG
                     VWNRIYRGVLVQHLDELKAAAPGHEIVYVPSHRSHMDYLLLSYLLYTHGVVPPHIFAG
                     INLNLPVVGTLLRKGGAFFARRSFRGNALYAAVFREYMAQLIAGGYSIEYFIEGGRSR
                     TGRLLPPKGGSLAMTVRAYLRQPSRPVLFQPIYIGYEKLMEGRSYLDELTGKPKEKES
                     IWQLLGGIPKVLRTNYGQVVVNFGERIRLDELLAEQAPDWNGKAVEEDEKPGWLADTV
                     DALAQKIQVNVNRAADVNPINLLALALLS"
BASE COUNT         1856 a         4189 c         3985 g         1672 t
ORIGIN      
        1 aggcgcgact cgcggtacat gtcggcgccc ttccacagca cgttgccgat gcccttgtgc
       61 atcgggctgt gcgggtcttc ctcggtctcg cacttggcgt ggtgcttgcg atggatcgcc
      121 gcccactcgc gggtgatcat cgacgtggtc agccagctcc agaaacggaa gaagtgggcg
      181 atgaccggat ggaagtcgac gccgcggtgc gcctggctgc gatgcaggta cagggtgacc
      241 gtgaagatgg tgatctgggt gaccaccagc aggtaggcca gcatggccca ccaaccgaac
      301 tgcaacaggc cgccggaaag gaattcaagg agggacgcgg gcatcgctgg ctcgtggaag
      361 gtgccgggag gacacgtgtg catcatggtg ccacgccggc gcgaacgcca tccctgcgcc
      421 ggcgcatggt gactgccggg ttcagtcccg cgcacgttga ccggcctgcg cgacaatgcg
      481 ccatgcctga actgcccgaa gtcgagacca cccgccgcgg cctgctgccg cacgtcgccg
      541 gacgccacgt ggaccacgtg ctgttgcgcc gccccgacct gcgctggccg atcccgcgcg
      601 aggtcgagaa cctgttgccg gggcagcgca tcgacgcgat ccgccggcgc gccaagtacc
      661 tgttgctgga taccgaggcc ggcagcgcgc tgctgcacct gggcatgtcc ggcagcctgc
      721 gggtgctgcc gtcggacacg ccggtgcggg cacacgacca tgtcgacatc gccctcgtct
      781 gtgcggatgg aatgcccgac cgggtgctgc gcttcaacga cccgcgccgc ttcggctgcc
      841 tgctctggca agcacccggc accgtgcatc cgctgctggc ggatctcggc cccgagccgc
      901 tgtcggacga cttcgacggg gatgtcctgt tcgcgctgag ccgcgggcgc agcgcgccgg
      961 tgaagtcctt cctgatggac caggcggtgg tcgtcggggt gggcaacatc tacgcggcgg
     1021 aggccttgtt catggccggc atctcgccgc tgcgcgccgc cggcaaggtc tcccgcgaac
     1081 gctacgcggc gctggcggat gcggtgaagg cgatcctggc gcatgccatt gcccgcggcg
     1141 gcaccaccct gcgcgacttc ctcagcccgg acggcgcgcc cggctacttc gagcaggaac
     1201 tgaccgtgta cgggcgcggc ggcgagccct gccggcgctg cgggcgtccg ctgaagcagg
     1261 cattgatcgg ccagcgcgct tcggcctggt gcgggcactg ccagcgctga cgccgcgcgg
     1321 gcggcatggc ctttggttat agtgccggct cccccgggtg cccctgttgc cgcaatgacc
     1381 gaagccggtg agatcaccgt cctgctcgat gccgcgcgcg atggcgaccg cggcgcgctg
     1441 gatcgcgtgc tcgccacgct gtaccaggaa ctgcacacga tggcgcgccg gcaactggcc
     1501 ggccagcagg gccacacgct ggatgccacc tcgctggtcc acgaggccta cctcaagctg
     1561 atcggccgcg gcacgggcgc cgcgcagttc gacgaccgcg cgcacttctt cgcctacgcg
     1621 gcctcggcga tgcgctcggt ggtggtcgat tacgcgcgcc agcggctggc gcagaagcgt
     1681 ggcggcgacc tgcaccgggt caccgacctg cccgaggaca tcgagggcgg gatccgcctg
     1741 gacgaggaca tgctgggcct ggacaccgcg ctgacccgac tgaccgcggt cgatccgcgg
     1801 ctgacccacg tggtcgaact gcgttacttc gccggcctgt ccgaactgga gatcgccgcg
     1861 ctgctgaagc gctccgagcg cagcgtacgg cgcgactggc agaaggcgcg gatgttcctg
     1921 ctcgcctccc tgcgggacac gcccgccgcc tgagggcggg gcgccggcgc tgcgcgatgg
     1981 acgccgaacg ctggcaacgc ctgtcgccgc tgctcgatgc cctgctcgag ctcgcccccg
     2041 agcaacgcag cgaacacctg gccgcactgc gccgcgaaga ccccgtgctg gccgatgaac
     2101 tggcgggcct gctcgccatg gaagcggacg acgaggattt cctgagcgaa ccggtggtcg
     2161 agctgccctc cggcgcacgg cccggcgccc gcatcggccc gtaccggctg gaccggttga
     2221 tcggcgaagg cggcatgggc caggtctggc tgtcggtgcg cgcggatggc ctctacgagc
     2281 gcaaggtcgc gctgaagctg ctgcgtcccg gcctggcgga tccgcgcctg cgccagcgct
     2341 tcgaccgcga gcgcgaaatc ctggcgcgat tcgcgcaccc gcacatcgcg cggctgctgg
     2401 atgccggcat cgacaaggac ggacaaccgt acctggcgct ggaatacgtc gagggcgagc
     2461 cgctcaccga ctactgccgc acccgccagc tggatatcgc cgcgcgcctc gacctgttcc
     2521 gccaggtctg cgaagcggtc agccacgcgc atgcgaacct gatcgtgcat cgcgacctga
     2581 agccgtcgaa catcctggtc accccggccg ggcatgtgcg cctgctcgat ttcggcatcg
     2641 ccaagctgct cgacgtcgat ccgctcccgg tcgagcagac ccgcaccggc gtgcgcacct
     2701 tcaccctgca ctacgccgcg ccggagcaga tccgcggcga gccggtcacc accatgaccg
     2761 acgtgtattc gctcggcgtg gtgctctacg aattgctcac cgggagcaag ccgtaccggc
     2821 tcaagcgcca gaccgatgcc gaatgggaag aggcgatcct cgacggcgag ccgctcaaac
     2881 cgtcgcaggt ggcggcgcgc gcgggcgaag cgtgcatccg cccgtacacc ccgcaacgac
     2941 tggcgcgcgc actgtccggc gacctcgaca acatcgtgct gaaggcgctg cgcaagcgcc
     3001 cggagcagcg ctacgtctcc gccgaggcac tgtcgcagga cctgctgtgc tacctgcgcg
     3061 ggcggccggt gtcggcgcgc ggcgagggca tggcctatcg cagccgcaag tacctgcatc
     3121 ggcatcgctg gacgattgcc gcggcgacca ccgtgctggc ggtgctgttc accgcgctgg
     3181 gcgtggtcgg ctggcaggcg caacgggcga tgcgcgaggc cagccgcgcg caggcgatgc
     3241 agggtttcgt ggccgacctc ttcgagagcg cggggacctc gtccgccagc gaaccgatcg
     3301 acctgcgcaa gctgctggac atgggcatcg tccgcggcga acgcagcctg gtgcggcaac
     3361 cgcaggcacg cgcggaactc tacggcgtgg tcgcgcggct gcggctgggg ctgggcgact
     3421 accgcgaggc caacaccctg ctcgacaagc aggaggtcct gctggccaac ctcccggacg
     3481 cgccggtcag cctgcgcctg gaggccgcca cgctgcgtgg caatgcctac cgccagctgg
     3541 gcaacctgcc gggttgcctg caggtcatgc agcgcatgga agacacggcc cggcgggagg
     3601 aagaacgcct gcccacgcaa gccgccgcct tctattcgca gcaggggcgc tgccaccagg
     3661 agcttgacca gagcacgctg gcgaaccggc tgtacatgcg ctcgctgcaa ctgcggcgca
     3721 agcagggcga cgacgtgggc gtggccgaaa acctcctgga catcgccaag atgcacgccg
     3781 ccgatggggc accggaggtc gccgtgccga tcctggccga tgcgctggcg gaactgcggc
     3841 gcaaggtcgg cgaccgccat ccgctggtga tcgacatgct gcgcagcctg tgcgcgctcg
     3901 atcgcgcgac cgaccggctg gacgatgccg tgcgccactg ccgcgagtcg caggcgctgg
     3961 ccgaagacct gcacggccgc gaccatcgcg ccagcatcga cgccaaccgc cagctggccg
     4021 cgctgtacgt ggacctgggc cgcttcagcc aggccgagtc gatcttcctg cagaccttcg
     4081 gctggatgcg cgcgcgcctg gaccgcaacc atccggacct ggcccgtgcc tacaacagcc
     4141 tggcgatcgt cgcctgggaa cgcggcgaca tcgagcgcgc gctggacttc cagcggcagg
     4201 cggtggccgg ctggaagacc gggcacaacg acggcctcac cgccaacggc ctgttcaacc
     4261 tggcgatgat cctgcacagc gtcgggcgcg accgcgaggc cctgccggcg gtacgcgaat
     4321 cgcgcgccct gcgggcgcaa cagttcggcg acacccatga gctggtcggc gacagcgacc
     4381 acctgctggg cgaggtcctg gccggcctgg gcgagccgga cgcggcgcgc gcggccctgc
     4441 agcaggcggt gcggctgacc ctggccggct acgggcccga gcattcgcat acccgccgcg
     4501 ccgagatctc gctggcccgg ctgcgcgcca acgagggcgc gcccgatgcc ctgctgcaat
     4561 tgaccgccta cgggcagtcc ggcagcggcg acatcgaaca gcgcaaggcc gcgtggctgg
     4621 cccgcgccta cgccgcggag gtggaatgcc gcacgggttc ccccacggcg cgcgccagcc
     4681 tggacacggt gctcaccgag atgcagctgg tcctgcccga aggcggcgcg atcccgcgcg
     4741 aggtcgccgc gatccgcaag gcatgcgtcc ggtagcaggg aaccgtcctg gggccagggc
     4801 tttgtaggag gggcgtcagc cccgacaaac aaacgcatcg gggctgaagc ccctcccaca
     4861 agagcgggtt ccgcccactc ctacaagggc aacggcgcct gcagcggctc gatggtgcct
     4921 tcgccgcgca tcaccttctt gaactccgcg cggctcaccg agacgtagcg ttcgttgccg
     4981 ccgatttcca cctgcggccc gtcctgcagc gcgcggccct gcgcatcgat gcgcacgttc
     5041 atcgtcgcct tcttgccgct gtggcagatc gtcttgatct cctgcaattc gtcggcccac
     5101 gccagcaggt actggctgcc ttcgaacaac tcgccgcgga aatcggtgcg caggccgtag
     5161 caaaggaccg ggatgcgcaa ggcatcgacc acttcgctca actgccaggc ctgcgccttg
     5221 ctgaggaatt gcgcttcgtc gaccaacacg cagtgcagcg gaccgtgcgc ggcgatgtcg
     5281 gcatcgatga tgtggcgcaa atcgtccgtg cgctcgaagg cgatgccctc cgcctgcagc
     5341 ccgatccgcg acgccaccgt gccgctgccc gcgcggaagt ccagcttcgg ggtgaggatc
     5401 gccacccgca tcccgcgctc gcggtagttg tgcgcggatt gcagcagggt ggtggtcttg
     5461 ccggcgttca tcgccgagaa atagaaatag agcttcgcca tgcacggatt ttacccgcgc
     5521 acaaggctgg cgagcgcacc gggcgcgccc gagaatgacg gcatgcagca tcccgcgttg
     5581 aatggcctga accccccgca gcggcaagcg gtcctgcaca ccgaaggccc gctactggtg
     5641 ctcgcgggcg ccggcagcgg caagacccgg gtgatcatcg agaagatcgc gcacctgatc
     5701 cacagcgggc gctatccggc caagcgcatc gccgcgatca ccttcaccaa caagtcggcg
     5761 cgcgagatgc gcgaacgcgt ggccaagcgc atccgtggcg atgccgcgca ggaactgacg
     5821 gtcagcacct tccacgcact gggcctgcgg atcctgcaga tcgaccatgc caagctcggc
     5881 ctgcgccgcg ggttctcgat cttcgacgcc gacgattccg cgtcgcaggt gaaggacctg
     5941 ctgggcccgg gcaccaagcc cgatgcgatc gacgcgatga agcaactgat cagccgcgcg
     6001 aagaacgcgg gactgtcgcc ggaacaggca gccgcggcgg cactgaccgg acgcgaacgc
     6061 gaggccgccg cgctgtacgc gcgttaccag cagcgcctgg ccgcgttcaa cgcggtcgat
     6121 ttcgacgacc tgatccgcct gccggtgcag ttgctggaaa ccgatgccga cgcggtcgcc
     6181 ggctggcgcg agcgcatcgg ttatctcctc gtcgacgaat gccaggacac caacgacgcg
     6241 caataccgcc tgctgaaggc gatcgccggc gatgccgggc aattcacctg cgtgggcgac
     6301 gacgaccaga gcatctacgc ctggcgcggc gcgaatcccg acaacctgct ggcgctcggc
     6361 accgactatc cggcactcaa gatcgtcaag ctggagcaga actaccgctg cagcaaccgc
     6421 gtgctgcgcg cggccaacgc ggtgatcgcc aacaacccgc acgaacaccc gaagacgctg
     6481 tggagcgatg ccgccgacgg cgaacgcatc cgcgtctggg aatgccgcga cgccgcccac
     6541 gaggccgaca aggtcgccgg cgagatccag ttcgtggcgc agaagcacac accgccgaat
     6601 ggcgtgccgc cgtggagcga gttctgcatc ctgttccgcg gcaaccacca gagccgcgcg
     6661 ctggagaagt cgctgcagct gctgcgcatt ccgtaccacc tgagcggcgg caccgccttc
     6721 ctcgaccgcg gcgaagtgaa ggacgcactc gcatggctgc gcctgctcgc caatcccgac
     6781 gacgacgcgg cgttcctgcg cgcggtgcag tcgccgaatc gcggcgtcgg cgcgacctcg
     6841 ctgcagcgcc tcggcgaact ggccaaccac gcgcacctgc cgctgtcacg cgcggcggat
     6901 tcgatcaccc tggccaagca gttgccggcg cgtgcggcga atgcgctgca ggggttttcc
     6961 gacatcgtcc gcggcctgcg catcgacgcg cgcacgctgc cgccggcgga attggtgcgc
     7021 aagctcaacg aacgctcggg cctgctcaac gcgctgcgcg tgctgtgcaa ggacgagacg
     7081 cagttccagg cgcgccgtcg caacctggac gaactggccg actggttcga cgggccgaag
     7141 acctcgggtc cgggcgaact cgcggctgca ctggccttgc tgtcgcatgc cgacaagggc
     7201 gatgccggca accaggtgcg gctgatgtcc atgcacgcgg cgaagggcct ggaattccgc
     7261 ttcgtcttca tcgtcggcgt ggaggacggc aacctgccgc acgaagccag catcgaggaa
     7321 ggccgcatcg acgaggagcg ccggctgttc tacgtgggca tcacccgcgc gaaggagcag
     7381 ctatggctgt cgcatgcgaa ggaagcgcag cgctggggcg acaagctgcg gctcaagccc
     7441 agccgcttca tcgacgaact gccggcggcc gaactgcagc gcgatggtgc cgacccggtc
     7501 gccgatgccg cgcacaaggc ggaacggcgc agcgccggct tcgccaacat ccgcgccctg
     7561 ctgggcgagt gaacgcgcgc cgggccgtca tcgtcgccgg gtaaactcgg ccgcatatcc
     7621 aagagagcct gtccccatga gtgccgccat gcgtcctgcc gttgccgttt ccctgctcgc
     7681 cgccgcgctg tcgctggccg cgtgcaagcc cacctcgctg accgcgccca gcggcgccgc
     7741 cgccaagccg gcgacggcca cggccgccaa gcccgccaca ggcgacgtcg gcgccaacga
     7801 caacctcaat gccgtactgt gggtgcagcg cgcggccgag tacgaggcgg tgacgcagac
     7861 cgtgtaccgc gccgccgccg accagctgga cgccgcgttg aagcaaccca actgggacgc
     7921 cctggtgccc ggcgagcgcg gcaacgcggc gaccggcctg ccgccggcgg tggtcatgga
     7981 cgtggacgag accgtgctgg acaactcgcc ctaccaggcg cgcctggtgc acgacggcgc
     8041 cagctacgac gaagccacct gggacctgtg ggtcgccgag aagaaggcca agccggtgcc
     8101 cggcgtggtc gatttcgcca aggccgcggc cgcgaaaggc gtgaccatcc tctacatttc
     8161 caatcgcgcg gtgcacctga aggacgccac catcgccaac ctcagggccg aaggcctgcc
     8221 ggtgaaggac gacagcgtgt tcctgggcct gggcaccttc gtcgagggtt gcgagcagaa
     8281 cggcagcgag aagctctgcc ggcgcaagct ggccgggcag aagtaccgcg tgctgatgca
     8341 gttcggcgac cagttgggcg acttcgccga gatcgtgtcc aacacgccga aagaccgcga
     8401 cgcgctgtac gcggaatacg cggactggtt cggcgagcgc tggtacatgc tgcccaaccc
     8461 gacctacggc agctgggagc cggcggtgtt caacaacgcc tgggagcaac ccgctgccga
     8521 tcgccgcaag gccaagctcg acgcgctcga acttgctaaa taaatcaatc gtttgcagca
     8581 aattagttca tctattgcat taactgtcgg acgccggtag cctgcgctca tccggtttcg
     8641 gccggagcca tgccacaacc gttgtcctcg gcaggatgcc gactttgaag gaggcccccg
     8701 ggcctccttt tttcttgccg ccacgcgctt gcgcctgcag gccgccggca tcgacactcc
     8761 gtccacgcca tccgggaccc ctcccatgac gcaacgcttt gtcggccggc gcctgcactt
     8821 cgcgcttgcc gccgccttgt gcctcacgct gccgacggcc tgcgagcgcg gcggtgtcac
     8881 caccggccaa accatcggcg tggcccgcgt cgccagggtc gaggccgcga cccgtccgat
     8941 cgatgccgcc tacgtcctgc gcgaccgcct gctggcccat gacggtgcgg ggttcgccag
     9001 gctggcggtg cccgcggacc tgcacgcgcg gctggccacg ggctggaagg acggtcgcag
     9061 ccgctggccg ctcgacgaac tgccgctgga cgccaagttg ccggggatgc tggccacgct
     9121 gcaggcgccg ggtgcggaca aggcgctgat ggcaaccttc cgccgccagt tcgccggtgc
     9181 cgaccgcgac atcgaccagg cgatccgcac cctcgcgttg ttcggtggcg aatacgtgca
     9241 gaacgacggc gactacaccc cggaggagcg cgagcacatc ggccaggcga tcattgcgct
     9301 ccaggcctgg ggcctgcagg cgccgttcgc ggacaaggcc cgcgcacagc gcttcttcac
     9361 cgcgctgacc gccgccgccc gtcgcagcgg catcgagggc aaggccggca cggccgcttt
     9421 cggcaagctg ggcatgcggg ccagcctgac ccggctgtcg ccgttcctcg ccaccctgct
     9481 cgcccagttg cgccagcaat acgggctgga cgtcgatgcc agcctgcgcg cgctgaaggt
     9541 gaccctgttg cagcagaccg gcgacaccgc ccgcctgcgc ctgcagtacc cgctggccgg
     9601 ccgcgacatc gacgcggtgg tgccggcggt gcgcatcgac ggccactggt acctggccga
     9661 tttcgtccag cgcgcggacg ccagcctggc cgccaaaccg gcagtcgcgg gcggcaggaa
     9721 agcgtctccg ccataatggg cgcgatgcaa agccgcccac cccagcccag ccaggccagc
     9781 ctgttcgacg cgccgcccgc cccggagacc ccgtcggaag gcgccgcgcc ggccaccgat
     9841 ggcgccgatg gcgtgcccgc cgtcgaggcc gatccgctgc cctcgctgcc gctgccgggc
     9901 ttcctggcgg tgcccgaccc tgcgcccgcc gaagccgatg cggctgtcgg cgtgggtgcc
     9961 ggcatcgatc ccgtgacccc cagcctcgac atcgaccccg cgccgccggc cgacgccgcg
    10021 gagcacgctg cgccggaggc cgcgcccgcg ccacccccca tgcgtcctgc caaggccaag
    10081 cgaccgctgt gggcccgcat cctgggccgc atcgtcgatc cgtggctgaa gctggacatc
    10141 gagcccgggg atcccgatca gttccggcag ttcgatgacg gccgcccggt ctgctatgtg
    10201 ctggaggact acggcctgtc caacgcgttg atcctggagc gcgcctgccg cgaggccggc
    10261 ctgccctcgc cgctgcagcc gatcccgggc gatccgctgg gccgcaagcg ctcgtacgtg
    10321 gcgctgtcgc ggcggcatgc caatgcgctg ctgtcgctgg ggctggtgcc ggggccggag
    10381 cacaagaccc attccggttc gctggcgcgg ctgctggagg cgcaccgcgc gaatgcggcg
    10441 ctggacgtgc agctggtgcc ggtgtcgatc ttcgtcggcc aggcaccgaa ccggagcagc
    10501 ggctggttca gcgtgctgtt ctccgaaaac tgggcgatcg tgggccgctt ccgccgcctg
    10561 ctggcgatcc tgctcaacgg tcgcgacacc ctggtgcagt tcgccccgcc ggtgtcgctg
    10621 cgcggcatcg tcgccgagga cctgccgccc gaacgcaccg tgcgcaagct ctcgcgcgtg
    10681 ctgcgcacgc atttccgccg cctgcgcgag gtcgtggtcg gccccgacct gtcgacccgg
    10741 cgcatgctgg tggacaaggt gctggctgcc gaaccggtgc gcgaggcgat cgccgaccag
    10801 gcgaaacgcg acaagaccac tgccgatgcg gcctggcaga aggcgcacgc ctacgcctac
    10861 gaaatcgccg ccgactattc gcacacggtg gtgcgctcgg ccagcttcct gctgaccggt
    10921 gtgtggaacc ggatctaccg cggcgtgctg gtccagcacc tcgacgaact caaggccgcg
    10981 gcgcccgggc acgaaatcgt ctacgtgccc tcgcaccgca gccacatgga ctacctgctg
    11041 ctgagctacc tgctgtacac ccacggggtg gtgccgccgc acatcttcgc cggcatcaac
    11101 ctcaacctgc cggtggtcgg caccctgctg cgcaagggcg gcgcgttctt cgcccgccgc
    11161 agtttccgcg gcaatgcgct gtacgcggcg gtgttccgcg aatacatggc ccagctgatc
    11221 gccggcggct actcgatcga atacttcatc gaaggcgggc gctcgcgcac cggccgccta
    11281 ctgccgccga agggcggttc gctggcgatg acggtgcgtg cctacctgcg ccagccgagc
    11341 cggccggtgc tgttccagcc gatctacatc ggctacgaaa agctgatgga aggccgcagc
    11401 tacctggacg agctgaccgg caagccgaag gaaaaggaat cgatctggca gctgctgggc
    11461 ggcatcccca aggtgctgcg gaccaactac ggccaggtgg tggtgaactt cggcgagcgc
    11521 atccgcctgg acgaactgct ggccgagcag gcgccggact ggaacggcaa ggccgtcgaa
    11581 gaagacgaga agccgggctg gctggcggac accgtcgacg cgctggcgca gaagatccag
    11641 gtcaacgtca accgcgccgc cgacgtgaac ccgatcaacc tgctggcgct ggcgctgctg
    11701 tc
//
LOCUS       DAOOCX010000073        11432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5306_length_11432_cov_10.690780, whole genome shotgun
            sequence.
ACCESSION   DAOOCX010000073 DAOOCX010000000
VERSION     DAOOCX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5306_length_11432_cov_10.690780"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /gene="recD"
                     /locus_tag="PK399_08035"
     CDS             complement(<1..1605)
                     /gene="recD"
                     /locus_tag="PK399_08035"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HPW13106.1"
                     /translation="MSLLRALQQAGLLRTLDDALANTLRRLDPSTRDDVLAATALASL
                     AIAQGHAGFDPAQPRLLVDADLPWPDADAWLQQLAASRFVATPASPIVEAMAAPLVLE
                     HGLLYLRRYREYERRLALQLRRIASQPMPETGIEPIAPLFASLFPDAVDGDHQARAAA
                     LALRRTLLLITGGPGTGKTTTIARVLALRIAQAGDAGSTPLRFALAAPTGRAADRMAE
                     GLRRAVAQLAEHGIDQALLDALPAQASTLHRLLGTIPESPRFRHGKDNPLPFDIVVVD
                     EASMVDLPLMCKLVDAVADGAQLLLLGDPDQLPSVEAGDVLAAILAAAGDGDAMSEDD
                     GAALRPLLGDVAVDAAASALYGHRVHLQRGWRQDASLDLAPLADAVRNDDAGEALLLL
                     RGGALSNVHFHEDAEDPLQARPDLLAHFRALATHADPAQALQQANRVRLLTAVREGPQ
                     GARGLNVRIEAALSGRRIGSPPAWFLGRLLLITENSYRHGLFNGDVGICLADADGTPL
                     AWFFDNGRGEGGNGVRAFHPAALPAHE"
     gene            complement(1602..5243)
                     /gene="recB"
                     /locus_tag="PK399_08040"
     CDS             complement(1602..5243)
                     /gene="recB"
                     /locus_tag="PK399_08040"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HPW13107.1"
                     /translation="MSDARDPYLDIPLDGVQLIEASAGTGKTFTLATLVTRLVVERGL
                     RIGQVLAVTFTEAATQELRKRIRERLQLTLDLLDAPAADDDSAEAALTRQLLQSHRTR
                     SGESDAALRRRLHQATLETDLAAIFTIHGFCARVLREHALESGQGFDAATLLTNDRDL
                     RAELAADLWRFHAQDSAGADDLAALWSGGFEDLSKDLPALLREPVLLPPVADDGLVAG
                     IEAMQRDVLQAQGALQQAWNEHGEAFLDTLAAAIDADVMNRGQYKQAWLADLRQWLQA
                     FAHDPWLAGAPHPKLEKLTATALATGTKKGNADRTPASAPMSQAVSHYLDALLALDGL
                     LVRRRGNLLHRLRDEARTRLSLLKRQRRVQTYDDLIDGVADALAHDEAGTLVRQLRSQ
                     YAVALVDEFQDTDARQWAIFDAVFGSGSAQPALFVIGDPKQAIYGFRGGDVHTYLAAA
                     KVAASAPALSHNFRSRPAVLRAVAALYANAGDAAFIDPRIEFRAVEAGHARDDDFLRD
                     GQPAPALTVWRAPKPPVDEKGKQKPWSAGESREFATQACVAAIHGVLSAARAGIALVE
                     DEPVQPGDIAVLVRSHREATMIQQALALVGIAAVAAGKQSLFATGEARDVHVLLLALL
                     HSGDDGRLRTALSTVLVGVDALGIDHLEADADAHRDWQQRAMGWRERLLRGGPLALIN
                     DLCASHAERLLGLLDGERRMSNYLQLAELLQEAQRDALGLHGLVDWLGQRIASADAND
                     EAQLLRLESDARRVQIVTLHKSKGLEYPLVFLPFVGIGGKPPSPGRCCVAHDAASGQR
                     RLHWKLDVPEAGWDAASEAWKEEQRAEDARLLYVGLTRAEHALWIASGHFYNADKTAL
                     AKMLGDADALEQAGVVFDATYSPQPPPRLPAESDAAVPRARIATRRLNHDWWVYSFSQ
                     LAKADAGVETSSASTLPASGGNDEPDAAEDVVVDVAFDARFAGTRYGVALHAALEHAD
                     FAAWRDWRAGDAAPPGQATIIADALGQEGFAADVIDDGVALTTQFVGQTLTVALPEGV
                     RLCDVPIGERRPEIEFQFALRPTQVDALLQLLHAHGIVNERHGFGLRQTLEGLMTGLV
                     DLTYRHDGKWYVLDYKSNRLPAYDAAAMQRAMAHSEYDLQALIYTLALHRWLRFRLRD
                     GYDYARDFGGIRYVFCRGLDASRSDSPGIHAQRFPPELVDALDALFLPLPLAGEGGGE
                     GLLRAEASP"
     gene            complement(5240..8578)
                     /gene="recC"
                     /locus_tag="PK399_08045"
     CDS             complement(5240..8578)
                     /gene="recC"
                     /locus_tag="PK399_08045"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HPW13108.1"
                     /translation="MTPAPDFRLYHGNALDVLAGLLADELRKDVPGQSLLAPDTLLIP
                     QVAMRRWLQATLAARHGIAANLEFLTPGEFVARALDGNLGPSKDDLDLDTLHWRLHAA
                     LSDPALRAKPAMAQLAAYLADADPLKTWSLAGELANIFNKYQAWRRDWLLRWDAGADP
                     DDPQAILWRAVATGRQHRARRIQDYLARFSEGDALPAGLPKRLFAFGTLNISPDVLRV
                     LATQARVGTLHFYLPTPTKHYWGDLQSLGARLREGVDPFGDEARENPLLRDWGAAGKD
                     FMALLGSYDVVHPSGEIAAYADPAEGDHAGLLQRMQADLFHRRSEPAGTKRDGVEADD
                     PSLQFHACHTRLRELQVLHDRLRALLEDTRFVPPLQPREIAVLAPDIDPYVPYLDAVF
                     GGHGAGSAIPYALADASPLAGERLADVFLRLLALPISRFGLEEMLDLLASPPLAEAAG
                     LEAVDFERLHGWLHAAGARWGLDARHRAGQGAPADDAYTWQFALDRLLLGHASGSDDD
                     IDGIAPWPELEGGALDALDALLKLLRVLARHQRVLGEAMPPAQWRERLIGLLDALLPR
                     PPSTSGGQRALDRLRSLIDDFAKQAEKAGVDAPLAAEVVRAHFTAKLTEADTRAPLLT
                     GGVSFARMVPMRLLPFRAICLLGMNDGDFPRRDSAAGLNRLTADLGTDKRRHGDRSTR
                     DDDRYLFLQLFSAAQDVFYLSWLGADPRDGSTREPSALVSELLAAASAYHADPGNAER
                     NLVLGHPLQPFSPAAFGAGDPRRFSYRGQWHPAAGRLSGARDRLPPWFDAATALASPV
                     VGETELSLDALRRFLQAPAEQFLRQRLGLRLPELDAAGEDIEPLHAPARGLERMVLQR
                     AVFDRLLEGDDAEAMQPALRARGLLPSGPLGTRGLAQVIREVAPYAQAFAQWRGEGEP
                     TSLAVDVDIEGVRLHGRLGDVWPNGIVRLRFGTPNGPSSIRNGLDWLLASAAGLDVPF
                     VEVQEDVDAGIDAHLRARIAQDDAVAALRSLIALRREGLQQPLPFAPYSGWELFATRD
                     DVASGLKKAAGKWRGNERGWAEGDIDAIRLALRGRDPFLDAASLHDFARISGIVFGAV
                     TRGMPMAIELADAPLPDDDAEDAA"
     gene            8800..9600
                     /locus_tag="PK399_08050"
     CDS             8800..9600
                     /locus_tag="PK399_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW13109.1"
                     /translation="MIDASLPAVRLQAVRLDRGGRAILSGLDLLAPKGSITAVLGPSG
                     SGKSTLLAALTGELVPTSGTLQVFGAPVPRSARGLLTMRKRLGVLLQGNGLLTDLTVA
                     ENVALPLRAHTTLPEPVLQALVRMKLHAVGLLAAADAWPRELSGGMARRVALARALAL
                     DPPLMLYDEPLTGLDPIASGVITALIQRLNRSLGLTSIIVSHHVRETLPICDQAVVIA
                     EGRIVFAGTPEALQATTDPLLRQFLDGQADGPIAFDARPFDAHARDAA"
     gene            9600..10349
                     /locus_tag="PK399_08055"
     CDS             9600..10349
                     /locus_tag="PK399_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HPW13110.1"
                     /translation="MAFAESIRSIGRGGLFALTMLRGSLPTRDFLAELVREIYKVGAR
                     SLPIIAVGGAFVGLVLTLQGYRTLSTYGAVDALSTLLGLSLYRELAPVLTALLFIGRA
                     GSSIAAELGLMRATDQIKALELMAIDPVAKVVAPRFWAAVLTMPLLAGIFVSLAISAG
                     WFQAVQVLGIDNGSFWSGLRGSVDFWDDFGVSMLKSAVFGGIAALVAAYVGFHAEPTI
                     EGTSVATTRAVVNASLLVLVFNFILSALLFR"
     gene            10367..10906
                     /gene="mlaD"
                     /locus_tag="PK399_08060"
     CDS             10367..10906
                     /gene="mlaD"
                     /locus_tag="PK399_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HPW13111.1"
                     /translation="MAIRGPRLEFAVGAFLLLALASLLVLALASTNRQFGFGGDTYPL
                     VAKFTQLGQLRANAPVKIGGVVVGQVSKIDLDPKTFQSVVTLSIDGRYQDLSADTSAG
                     IFTSGLLGESYIGLSPGGDPESLKPGDEIAFTQPAVDLLQLVGKYMFSGSGKPAGDDA
                     ATEAPADPAALPAPEGQTP"
     gene            10903..>11432
                     /locus_tag="PK399_08065"
     CDS             10903..>11432
                     /locus_tag="PK399_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW13112.1"
                     /translation="MSTLFRSRPIASVVLVGLLATAPSFTATAATPPAATAQQGAASR
                     VVVDSAGRVLATLDSRRAEFKANPVALNQFIKTEFTRSFDSDYAARLVLGVHGRSAAP
                     ADVADFAAAMADNLTARYGASLLDFNTKLQVKVTSETPLPGGRGVRVSTQMLRQGGSP
                     IPVDYLLRNVNGQWKL"
BASE COUNT         1705 a         3972 c         4007 g         1748 t
ORIGIN      
        1 ttcgtgcgcg ggcagcgcgg cgggatggaa ggcgcgcacg ccgttgccac cttcgccgcg
       61 gccgttgtcg aaaaaccagg ccagcggcgt accgtcggca tcggccaggc agatgccgac
      121 gtcgccgttg aacaggccgt gtcgatagct gttctcggtg atgagcagca ggcggccgag
      181 gaaccatgcg ggcgggctgc cgatgcggcg gccggagagt gcggcctcga tgcgtacgtt
      241 gaggccgcgt gcgccctgcg ggccttcgcg cacggcggtg agcaggcgca cgcggttggc
      301 ctgctgcaag gcctgcgcgg gatcggcgtg cgtggccagc gcgcggaaat gcgcgagcag
      361 gtcaggtcgc gcctgcagcg gatcttccgc gtcttcgtgg aagtgcacgt tggaaagcgc
      421 gccaccacgc agcagcaaga gcgcttcgcc ggcatcgtcg ttgcgcacgg cgtccgcgag
      481 tggcgcgagg tccagtgatg cgtcctggcg ccaccctcgt tgcaggtgga cgcggtggcc
      541 gtacagggcg ctggcagcgg catcgacagc cacatcgccg agcaatggcc gcagcgcggc
      601 gccatcgtct tcggacatgg catcgccatc gcccgccgcg gccaggatcg cggccagcac
      661 gtcgccggct tcgaccgagg gcaactggtc cggatcgccg agcaggagga gctgcgcgcc
      721 atcggccacc gcgtcgacca gcttgcacat cagcggcagg tcgaccatcg aggcttcatc
      781 caccaccacg atgtcgaacg gcagcgggtt gtccttgccg tggcggaaac gcggcgattc
      841 cggaatggtg ccgagcaggc gatgcagggt gctggcctgc gcaggcaagg cgtcgagcaa
      901 tgcttggtcg atgccgtgtt cggccaattg ggcaacggcg cgacgcaggc cctcggccat
      961 gcgatcggcg gcgcgaccgg tgggtgcggc cagggcgaag cgcaacggcg tgctgcctgc
     1021 gtctcctgcc tgcgcgatgc gcagggcgag cacccgggca atcgtggtgg tcttgccggt
     1081 gccgggacca ccggtgatca gcagcaacgt gcggcgcagc gcgagcgcgg cggcgcgggc
     1141 ctggtgatca ccatcgacgg catccgggaa cagcgacgcg aacaacggcg cgatcggttc
     1201 gatgcctgtt tccggcatgg gttgcgacgc gatgcgccgg agctgcagcg cgaggcggcg
     1261 ttcgtattcg cggtagcggc gcaggtacag caagccgtgc tcgaggacga gcggcgcggc
     1321 catcgcctca acgatgggcg atgccggggt ggcgacgaag cgcgatgcag cgagttgctg
     1381 caaccaggca tcggcatccg gccagggaag gtcggcgtcg accagcaggc gcggttgcgc
     1441 cggatcgaag ccggcatggc cctgcgcgat cgcgagcgag gcgagcgcag tcgcggccag
     1501 cacgtcatcc cgcgtcgatg gatcgagacg acgcaaggtg ttggccagcg catcgtccag
     1561 cgtgcgcaac aggccggcct gttgcagggc gcgcagcagg ctcatggtga agcctccgcg
     1621 cgcaagagcc cctcaccccc accctctccc gcaagcggga gagggagaaa gagcgcgtcg
     1681 agcgcatcga ccaattccgg cgggaagcgc tgcgcgtgga tgccgggcga gtcgctgcgg
     1741 ctcgcgtcca ggccgcgaca gaagacatag cgaatgccgc cgaaatcgcg ggcgtagtcg
     1801 tagccgtcgc gcaggcggaa gcgcagccag cgatgcaggg cgagcgtgta gatcagcgcc
     1861 tgcaggtcgt attcgctgtg cgccatcgcg cgctgcatcg ccgcggcgtc gtacgcgggc
     1921 aggcgattgg acttgtagtc cagcacgtac cacttgccgt cgtgccggta ggtcaggtcg
     1981 accaggccgg tcatcaggcc ttcgagcgtc tgccgcaggc cgaagccgtg gcgctcgttg
     2041 acgatcccgt gcgcgtgcaa caactgcagc agcgcgtcca cctgggtcgg acgcagcgcg
     2101 aactggaatt cgatttccgg gcggcgctcg ccgatcggca cgtcgcacag gcgcacgcct
     2161 tccggcaagg cgacggtcag cgtctgcccg acgaactgcg tggtcagcgc gacgccgtcg
     2221 tcgatgacgt ccgcggcgaa cccttcctgg ccgagtgcgt ccgcgatgat cgtcgcctgt
     2281 ccgggcggcg cggcatcgcc tgctcgccaa tcgcgccagg cggcgaagtc ggcgtgttcc
     2341 aacgcagcat gcaaggccac gccgtagcgc gtgccggcga aacgcgcatc gaatgcgaca
     2401 tcgacgacca catcctccgc tgcatccggt tcgtcattgc cgccgctcgc cggcaaggtg
     2461 gacgcgctgc tggtctccac accggcatcg gccttggcca attggctgaa gctgtacacc
     2521 caccagtcgt gattgagccg gcgcgtggcg atgcgcgcgc gcggaaccgc cgcatccgat
     2581 tcggcgggca ggcgcggcgg tggttgcggc gaatacgtcg cgtcgaacac gacgccggcc
     2641 tgttccagcg catcggcatc gcccagcatc ttcgccagcg cggtcttgtc ggcgttgtag
     2701 aaatgcccgc tggcgatcca cagcgcgtgt tcggcgcggg tcaggcccac gtagagcagg
     2761 cgcgcgtcct cggcgcgttg ctcctccttc caggcctcgc tggcggcatc ccaccctgct
     2821 tccggcacat ccagcttcca gtgcaggcgg cgctggccgc tggcggcgtc gtgcgccacg
     2881 cagcagcgcc cgggcgacgg cggcttgccg ccgatgccca cgaacggcag gaacaccagc
     2941 ggatactcca gccccttgct cttgtgcagg gtgacgatct gcacgcggcg cgcatcggat
     3001 tccaggcgca gcagttgtgc ctcgtcgttg gcatcggcgc tggcgatgcg ctggcccagc
     3061 cagtcgacca ggccgtgcag gcccaacgca tcgcgctgcg cttcctgcag cagttcggcc
     3121 agttgcaggt aattgctcat gcgccgctcg ccatccagca ggccgagcag gcgttcggca
     3181 tggcttgcgc agagatcatt gatgagtgcc agcggaccac cgcgcagcag gcgttcgcgc
     3241 cagcccatcg cgcgctgttg ccagtcgcgg tgcgcgtcgg catcggcctc gagatggtcg
     3301 atgccgagtg cgtccacgcc gaccagcacg gtggacagcg cggtgcgcag ccggccgtcg
     3361 tcgccgctgt gcaacagggc gagcaacaac acgtgcacgt cgcgcgcctc gccggtggcg
     3421 aacaggctct gcttgccggc ggcaaccgcg gcgatgccga ccagcgccag cgcctgctgg
     3481 atcatcgtgg cctcgcgatg cgaacgcacc agcaccgcga tatcgcccgg ctgcacgggt
     3541 tcgtcttcga ccagcgcgat gccggcacgc gccgccgaca gcacgccatg gatcgcggca
     3601 acgcaggctt gggtggcgaa ctcacgcgat tcgcccgcgc tccacggctt ctgcttgccc
     3661 ttctcgtcga ccggcggctt cggtgcacgc cagaccgtca atgcgggcgc gggctggcca
     3721 tcgcgcagga agtcgtcgtc gcgcgcgtgg ccggcctcga cggcgcggaa ttcgatgcgc
     3781 ggatcgatga aggcggcatc gccggcattg gcgtagagcg cggcgacagc gcggagcacg
     3841 gccgggcgcg aacggaagtt gtgcgacagc gccggcgcgg acgccgcgac cttggcggcg
     3901 gcgaggtagg tatgcacatc gccgccgcgg aaaccgtaga tcgcctgctt gggatcaccg
     3961 atcacgaaca atgccggctg cgcgctgccg ctgccgaaca cggcatcgaa gatcgcccac
     4021 tggcgcgcat cggtgtcctg gaattcgtcg accagggcca ccgcgtactg gctgcgcagt
     4081 tgccgcacca gcgtgccggc ttcgtcatgc gccagcgcat cggcgacgcc gtcgatcagg
     4141 tcgtcgtagg tctgcacgcg gcgttgccgc ttgagcaggg acaggcgcgt gcgcgcctcg
     4201 tcgcggagcc gatgcagcag gttgccgcgc cggcggacca gcaggccatc gagtgccagc
     4261 aacgcatcca ggtagtgcga caccgcctgg ctcatgggtg ccgatgccgg cgtcctgtcc
     4321 gcgttgcctt tcttggtgcc cgtggccaac gcggtcgcgg tgagcttctc cagcttcgga
     4381 tgcggcgcgc cggccagcca cggatcatgg gcgaaggcct gcagccactg gcgcaggtcg
     4441 gcgagccacg cctgcttgta ctgcccgcga ttcattacgt ccgcgtcgat cgcggcggcg
     4501 agcgtgtcca ggaacgcttc gccgtgctcg ttccacgcct gctgcaacgc gccctgcgcc
     4561 tgcagcacgt cgcgctgcat ggcctcgatg cctgcgacca ggccatcatc ggcgacgggt
     4621 ggcaacaaca ccggctcgcg caacagtgcc gggagatctt tcgacagatc ctcgaaacca
     4681 cccgaccaca acgccgccag gtcgtccgcg cccgcgctgt cctgcgcatg gaagcgccag
     4741 agatcggcgg cgagttccgc gcgcaggtcg cggtcgttgg tcagcagggt ggcggcgtcg
     4801 aagccctgcc cgctttccag cgcgtgttcg cgcagcacgc gcgcgcagaa gccgtggatg
     4861 gtgaagatcg cggccaggtc ggtttccagc gtggcctggt gcaggcggcg gcgcagcgcg
     4921 gcatcgcttt ctccactgcg cgtgcgatgc gattgcagca gttggcgggt cagcgcggct
     4981 tccgcgctgt cgtcgtcggc cgctggcgca tcgagcagat cgagggtgag ttgcaggcgc
     5041 tcgcggatgc gcttgcgcag ttcctgcgtg gccgcttcgg tgaaggtgac cgcgaggacc
     5101 tggccgatgc gcaggccgcg ctcgaccacc agccgcgtca ccagcgtggc cagggtgaag
     5161 gtcttgccgg tgccggcgct ggcctcgatc agctgcacgc catccagcgg aatgtccagg
     5221 tacggatcgc gcgcgtcgct catgcggcgt cctccgcatc gtcgtccggc agcggggcgt
     5281 cggcgagttc gatcgccatc ggcatgccgc gggtgacggc gccgaagacg atgccgctga
     5341 tgcgcgcgaa gtcgtgcagc gatgcggcat cgaggaacgg atcgcggcca cgcagggcca
     5401 accggatcgc gtcgatatcg ccttctgccc aaccgcgttc attgccgcgc catttgccgg
     5461 cggccttctt caggccgctg gccacgtcgt cgcgggtcgc gaacagttcc cagccgctgt
     5521 agggcgcgaa cggcaacggc tgttgcaggc cctcgcgacg cagcgcgatc aggctgcgca
     5581 gcgcggcgac ggcatcgtcc tgcgcaatgc gcgcacgcag atgcgcatcg atgccggcat
     5641 cgacatcctc ctgcacttcc acgaacggca catcgagccc ggcggcgctg gccagcaacc
     5701 agtccagccc gttgcggatc gaggacggcc cgttcggcgt gccgaagcgc aggcgcacga
     5761 tgccgttcgg ccacacatcg ccaagccggc catgcaggcg aacaccctcg atgtcgacat
     5821 cgaccgcaag cgatgtcggc tcgccttcgc cacgccattg cgcgaaggcc tgcgcatacg
     5881 gcgcgacctc ccggatcacc tgcgccaatc cgcgcgttcc cagcggtccc gacggcagca
     5941 ggccgcgcgc acgcaacgcg ggctgcatcg cctcggcatc gtcgccttcc agcagtcggt
     6001 cgaacaccgc gcgttggagg accatgcgtt cgagcccacg cgcgggtgcg tgcagcggtt
     6061 cgatgtcctc gccggcagcg tccagttccg gcaggcgcag gcccaggcgc tggcgcagga
     6121 actgttcggc cggcgcttgc aggaagcgcc gtagcgcatc gagcgagagt tcggtttccc
     6181 cgacgaccgg cgatgcgagt gccgtcgccg catcgaacca cggcggcagc cggtcgcgcg
     6241 cgccggacag ccgacctgcc gccggatgcc actgcccgcg atagctgaaa cgccgcggat
     6301 ccccggcgcc gaacgcggcc ggcgaaaacg gctgcaacgg atgccccagc acaaggtttc
     6361 tttccgcgtt gcccggatcc gcgtgatacg cgctggcggc ggccagcaat tcgctcacca
     6421 gggcggacgg ctcgcgcgtg ctgccgtcgc gcggatccgc accgagccag ctcaggtaaa
     6481 acacgtcctg cgccgcggag aacagttgca ggaacaggta gcggtcgtcg tcgcgggtgg
     6541 agcggtcgcc gtggcggcgc ttgtcggtac cgagatccgc cgtgaggcga ttgagcccgg
     6601 ccgcgctgtc gcggcgcggg aagtcgccat cgttcatgcc gagcaggcag atcgcgcgga
     6661 acggcagcag ccgcatcggc accatccgcg cgaagctcac gccgccggtg agcaggggcg
     6721 cgcgggtatc ggcttcggtc agcttggcgg tgaaatgcgc acgcacgacc tcggcggcga
     6781 gcggcgcatc gacaccggct ttctccgcct gcttcgcgaa atcgtcgatg aggctgcgca
     6841 ggcgatccag cgcgcgttgc ccgccgctgg tcgaaggcgg gcgcggcagc agcgcatcga
     6901 gcaggccgat gaggcgttca cgccattgcg cgggcggcat cgcctcgccg agcacgcgct
     6961 ggtggcgggc cagcacccgc agcagtttca gcaaggcatc gagcgcatcc aacgcgccgc
     7021 cttccagttc cggccacggc gcaatcccgt cgatgtcgtc gtcgctgccg ctggcatggc
     7081 ccagcagcaa gcggtccagc gcgaactgcc aggtgtaggc atcgtccgcg ggggcgcctt
     7141 ggcccgcgcg atgccgcgca tccagccccc agcgcgcacc ggccgcatgc aaccagccat
     7201 gtaggcgctc gaaatcgacc gcctccaacc cggccgcttc ggccaggggc ggactggcca
     7261 gcaggtcgag catctcctcc agcccgaagc gcgatatcgg gagcgcaagc aggcgcagga
     7321 acacgtcggc caggcgctcg ccggccagcg ggctggcatc ggccagcgcg tagggaatcg
     7381 cactccccgc gccgtggccg ccgaacaccg catccaggta cggcacgtac gggtcgatgt
     7441 cgggcgccag cacggcgatc tcgcgcggct gcagcggtgg aacgaaccgc gtgtcttcca
     7501 gcaatgcgcg caggcggtca tgcagcacct gcaattcgcg caggcgggtg tggcaggcgt
     7561 ggaattgcag gctgggatcg tcggcctcca cgccatcgcg cttcgtgccc gcgggttcgc
     7621 tgcggcgatg gaacaggtcg gcctgcatgc gctgcagcag gccggcgtgg tcgccttcgg
     7681 cgggatcggc atacgcggcg atctcgccgg acggatgcac cacgtcgtaa ctgcccagca
     7741 gggccatgaa gtccttgccg gcggcacccc agtcgcgcag caacgggttc tcgcgggcct
     7801 cgtcgccgaa cggatcgacg ccctcgcgca ggcgtgcgcc gagcgattgc aggtcgcccc
     7861 agtaatgttt cgtcggcgtc ggcaggtaga agtgcagggt gccgacccgc gcctgcgtgg
     7921 ccagcacgcg cagcacgtcc ggcgagatgt tgagcgtgcc gaaggcgaac aggcgcttcg
     7981 gcagtcccgc gggcagtgcg tcaccttcgc tgaagcgggc gaggtagtcc tggatccgcc
     8041 gcgcgcgatg ctggcggccc gtcgcgacgg cgcgccacag gatcgcctgc gggtcgtcgg
     8101 gatcggcgcc ggcatcccag cgcagcagcc aatcgcgacg ccaggcctgg tacttgttga
     8161 agatgttggc cagttcaccg gccagcgacc acgttttcag cgggtcggca tcggccaagt
     8221 aggcagcgag ctgcgccatc gcgggcttcg cccgcagcgc aggatcggac agcgcggcat
     8281 gcaggcgcca gtgcagggtg tcgaggtcga gatcatcctt cgacgggccg aggtttccat
     8341 ccagcgcacg cgcgacgaat tcgcccgggg taaggaactc gaggttcgcg gcgatgccgt
     8401 ggcgcgcggc cagcgtggcc tgcagccagc gtcgcatcgc cacctgcggg atcagtaggg
     8461 tgtctggcgc cagcaacgat tggccgggca cgtccttgcg cagctcgtcc gccagcaggc
     8521 cggccagcac gtccagcgcg ttgccgtgat acaggcggaa gtcgggggcg ggcgtcatcg
     8581 gtcatccatt gtgccggagc gggtcgcgcg ggctgcgacc ggcggcaggc ctgcgctgcg
     8641 agtccgttgc ggaatgcgtc gttcagcctg gggatgccct ccacgtcgcg gacaggtaac
     8701 cttcctgtac caatcggttc agccacagtg cgtttcgacg tggctcgcgc gttcttcgat
     8761 tccgcgcacc aacccgccaa cggaccccct gcttcgccga tgatcgatgc ctcccttccc
     8821 gccgtgcgcc tgcaggccgt ccgccttgat cgtggcgggc gcgcgatcct gtcggggctg
     8881 gacctgctgg cgcccaaggg cagcatcacc gcggtgttgg gtccctccgg cagcggcaag
     8941 tccaccctgc tggcggcatt gaccggcgag ctggtgccaa ccagcgggac gctgcaggtg
     9001 ttcggcgcgc cggtgccgcg cagcgcccgc gggttgctca cgatgcgcaa gcgcctgggc
     9061 gtgctgctgc agggcaatgg cctgctcacc gacctgaccg tggccgagaa cgtggcgctg
     9121 ccgctacgcg cgcatacgac gttgccggaa ccggtgctgc aggcattggt gcggatgaag
     9181 ctgcatgcgg tcggcctgct cgccgccgcc gatgcctggc cacgcgaact ctccggcggc
     9241 atggcccggc gcgtcgccct ggcccgcgcg ctggccctgg atccaccgct gatgctctac
     9301 gacgaaccgc tgaccggcct tgatcccatt gcctcgggcg tcatcaccgc actgatccag
     9361 cggctcaacc gcagcctggg cctgaccagc atcatcgtca gccaccacgt gcgcgaaacg
     9421 ctgccgatct gcgaccaggc ggtggtgatc gccgaggggc ggatcgtgtt cgccggcacg
     9481 ccggaggccc tgcaggcgac caccgatccg ctgctgcggc agttcctgga tggccaggcg
     9541 gatgggccga tcgcattcga tgcgcgtccg ttcgacgcgc acgcacggga cgccgcctga
     9601 tggcattcgc cgaatcgatc cgttcgatcg gtcgcggcgg cctgttcgcg ctgacgatgc
     9661 tgcgcggctc gctgccgacc cgcgacttcc tggccgaact ggtgcgcgag atctacaagg
     9721 tcggcgctcg ctcgctgccg atcatcgcgg tcggcggtgc attcgtcggc ctggtgctga
     9781 ccttgcaggg ctatcgcacg ctttccactt acggtgcggt cgatgcgttg tcgaccctgc
     9841 tcgggctgtc gttgtatcgc gaactggcgc cggtgctgac cgcgctgctg ttcatcggcc
     9901 gcgccggcag ctcgatcgcc gcggaactcg ggctgatgcg cgccaccgac cagatcaagg
     9961 cgctggaact gatggcgatc gacccggtcg cgaaggtcgt cgccccgcgc ttctgggcgg
    10021 cggtcctcac catgccgctg ctcgccggca tcttcgtctc gctggcgatc agcgccggct
    10081 ggttccaggc ggtgcaggtg ctcggcatcg acaacggcag cttctggtcc ggcctgcggg
    10141 gcagcgtgga tttctgggac gacttcggcg tgtcgatgct gaagtcggcg gtgttcggcg
    10201 gcatcgccgc gctggtggcg gcttacgtcg gtttccatgc cgagccgacc atcgagggca
    10261 cctcggtggc gaccacccgc gcggtggtga acgcgtcgct gctggtgctc gtcttcaact
    10321 tcatcctttc cgccctgctg ttccgttgag caagagacct acacccatgg ccatccgcgg
    10381 accacgactc gaatttgccg tcggcgcctt cctgctgctg gcgctggcct cgctgctggt
    10441 gcttgcgctg gcctcgacca accgccagtt cggcttcggc ggcgacacct atccgctggt
    10501 cgccaagttc acccagctcg gccagttgcg cgccaatgcg ccggtgaaga tcggcggcgt
    10561 ggtggtcggc caggtcagca agatcgacct cgacccgaag accttccagt cggtggtgac
    10621 gctgtccatc gacggccgct accaggacct gtcggccgac acctcggccg gcatcttcac
    10681 cagcggcctg ctcggcgaaa gctacatcgg cctgtcgccg ggcggcgatc cggaatcgct
    10741 gaagccgggc gacgagatcg ccttcaccca gcccgcggtc gacctgctgc aactggtcgg
    10801 caagtacatg ttcagcggca gtggcaagcc cgcgggcgac gatgccgcca ccgaagcgcc
    10861 cgccgacccg gccgcgctgc ccgcccccga gggacagacc ccatgagcac attgtttcgc
    10921 agccgcccca ttgccagcgt cgtgctcgtc ggcctgctgg ccacggcccc ctcgttcacc
    10981 gccacggccg ccacgccgcc tgccgccacg gcgcagcagg gcgccgccag ccgcgtggtg
    11041 gtcgacagcg ccggccgcgt gctggccacg ctggatagcc gccgcgccga gttcaaggcc
    11101 aacccggtcg cgctgaacca gttcatcaag accgagttca cccgcagctt cgattccgac
    11161 tacgccgcgc gcctggtgct gggcgtgcac ggccgcagcg ccgcgcccgc cgacgtggcc
    11221 gacttcgccg cggcgatggc cgacaacctg accgcgcgct acggcgcgtc gctgctcgac
    11281 ttcaacacga agttgcaggt caaggtgacc tcggaaaccc cgttgcccgg cggtcgaggc
    11341 gtgcgcgtga gcacccagat gctgcgccag ggtggttcgc cgatcccggt cgactacctg
    11401 ctgcgcaacg tcaacggcca atggaaactg tt
//
LOCUS       DAOOCX010000074        11140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5476_length_11140_cov_8.878484, whole genome shotgun sequence.
ACCESSION   DAOOCX010000074 DAOOCX010000000
VERSION     DAOOCX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5476_length_11140_cov_8.878484"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="PK399_08070"
     CDS             complement(<1..1709)
                     /locus_tag="PK399_08070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPW13113.1"
                     /translation="MSRLSQAVGEALGAGSPLAERIPGFVARPAQQRLAMAVADAFEQ
                     RGVLLAEAGTGTGKTFAYLVPALLSGMKTIVSTGTRALQDQLYFRDLPRVCDALGTGL
                     KTALLKGRSNYLCLYRMEQAKGDPQLFKGGFSSREQVSQFQRVVAWSGRTKMGDLAEL
                     ESLPEDSPLLPQVSSTADNCLGTDCPRWGDCFVVQARQRAQTADLVVVNHHLLLADLA
                     LKQEGFGEILPGAQAFVVDEAHQLPELAAQFFGEGLGARPLVELARDALGECKDVAGS
                     LASVQEPARALEQAARALRASMDNLPVRGTAWRALDEVDIEPAFQSLADALQRMQDTL
                     EPLREAAPGFDACHLRARDQLGRLRRWLGEKVGSESPFETKGDSDPTFDADDRPPDTS
                     VHWYELTPRGFRLQRTPLDVSAPLRRHREESRAAWVFTSATLAVSGGFSHIAQRLGIN
                     EAVELLEPSPFDWDRQALCFLPTRLPEPASRDYGTALIATLLPVLQASGGRAFLLFAS
                     HRALREAAELLRDAPWPLFVQGSEPRHVLLQRFRESGNGVLLGTASFREGVDVAGDAL
                     SVVV"
     gene            complement(1726..>3182)
                     /locus_tag="PK399_08075"
     CDS             complement(1726..>3182)
                     /locus_tag="PK399_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPW13114.1"
                     /translation="VLGEMHETGLIDDKEYQRATKAPLGVTENAGSLAANRFPAYVDL
                     VRKQLARDYPADKLSGAGLSVMTGMSPAAQGYAEGAVARTLKSLSTKGRPPLQAGVVM
                     TDVHNGDVVAVVGSREFTEQGFNRAVDAKRPVGSLLKPFVYLLALAQPDKWSLASTIS
                     DAPVTVSLGRSRNWKPGNSDGRSHGTVRLLDALAQSYNQATVRLGMDVQPQRLAELTR
                     TLAGIESEGQPSLILGAVDQSPYAMAQLYQFLASGGEIQPLHAVRGVLDANGRAIKRY
                     DSVAPPAQKGDAVAARLVGVALQYAVSSGTGRPLVRDGLGRLAAAGKTGTSNDGRDSW
                     FAGYTGDHLAVVWVGNDQNKETGLYGATGGMRVWSDVFARLPSAPLQLSDEGLDWRWI
                     EGGHSTDPGCPGARRFAFVAGYAPGYQACVYAPPPALDEYGNPIPADPALDGEPGALE
                     RAGQAIRGWFGGDDPAKQVPVPADAPPATPAAQP"
     assembly_gap    3183..3282
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3283..4358)
                     /locus_tag="PK399_08080"
     CDS             complement(<3283..4358)
                     /locus_tag="PK399_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPW13115.1"
                     /translation="MPRIEDEDDLHDDDGDGTDSQGPVWRNRLLTWGLAAAALLLGFL
                     IPYMLYLNHQVGERFGKLRWQVPTRVYARPLVLRTGLAMDAQTLKTELDAASYRDGDG
                     RRAGTYDRNGARWRISSRGYDDVDGRVAPAILEVTLSGGDVAAIRDVAGKRALRSARL
                     DPARVATLYGQNQEERRLVRIGDVPALLVEGLQAVEDRDFNRHMGIDLGGMLRAAFVT
                     VKSGGDTKQGASTLTQQLARSGLLGIGKEQTVTRKVKEILFALLLEARYDKGTILEAY
                     FNQVDLGQRGAQSIRGVAAASEFWFGKELQDLSTEQIALMIGLVKGPSWYNPRRNPGR
                     ATERRNFVLGEMHETGLIDDKEYQR"
     gene            4486..6576
                     /locus_tag="PK399_08085"
     CDS             4486..6576
                     /locus_tag="PK399_08085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW13116.1"
                     /translation="MPLPAEARFWIARALGLWRRGWASLRTRGLAASWAGLLRQVRRR
                     PLPAHALYAPPATAFSPFVMPTSATPRASIVIPVYGAFAHTLACLRAIAAHPPAAAFE
                     VIVVDDASPDDSLPKLRAIDGLHLHARATNGGFIAACKDGASLARGEVLVFLNNDTVP
                     QPGWLDALLATFETHPAAGLVGAKLLYPDGRLQEAGGVVFADGSGWNYGRFEDPADCR
                     YTYLRDADYVSGAAIAIPRDLFARLGGFDARYAPAYYEDTDLAFKVRAAGRRVLYQPA
                     SVVVHDEGTTSGTDLGTGPKAAQVRNQAVFAETWRHALTAQLPAGSVPSPALLHRRQR
                     QVLVIDALTPQPDRDSGSLRLVNLMRLLIEEGAHVVFVPANRSADGEYTATLQQMGVE
                     CWHAPTMPRFPAWLREHGPRFDAAMVSRHYVASEMLPLLRKHAPQAKLLFDTVDLHYL
                     REKRAAELTADATALRAAMRTRELELALIRDADATLVVSRVERELLGIDAPGANVHVL
                     SNLHRAAAAGPSWALRDGLLFVGGFRHPPNVDAVLWFVREVFPRVRAARPTIEFHCVG
                     GDVPNEIARLADVPGVRVHGHVPDLQPLLAAARVSIAPLRYGAGVKGKVNQAMAHGLP
                     VVATTCAVEGMHLRDGEDVLVADDASEFADAVLRLLDDEASWSRLAVNGRANVAAHFS
                     LDAARATVRTLFVD"
     gene            complement(6590..8011)
                     /locus_tag="PK399_08090"
     CDS             complement(6590..8011)
                     /locus_tag="PK399_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13117.1"
                     /translation="MALLASVLFRQRIGNWIWPDPRAEALRTQAGVALAAGRLSVADG
                     SGARELYEAALALQPDQVEAREGLSRVALAALAQAEAHARGGREAQARIALRLARELD
                     APKARIDAAEALLRMDDAAQAGIVALLAEAEAAHVAGQLRDGVDAALPLYQRVLALQP
                     RNQRAVEGREDALSDLLQPVPGLLARGDLAGASSRIQAAERFDPGHVDLPELRAGLAQ
                     ATQQRSARIRQSLQRGQHAAAADACVALREVAASAVPDECSGAVVDGLLRDAATATAD
                     FHFDDTTRLLALVERLEGDPVQVQAARHRLQAARVGAAQLPRVVTSPRVKAQVRDLLA
                     QAEAARNRGDWLSPPGDSAWDRLREARALAPADPAVQGALEAMLPAARDCNATALRDN
                     DLGRAQVCLEAWRQLAPADAALAAAQRRLAERWLAIGEERLGAGELEATARALARARA
                     LDAATPGLPALQQRLERASSGAN"
     gene            complement(8119..10173)
                     /locus_tag="PK399_08095"
     CDS             complement(8119..10173)
                     /locus_tag="PK399_08095"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPW13118.1"
                     /translation="MRDRADVLLDTLAILSTLNADAEVMAAALLDALVDIEPLAAEAK
                     IEIPERLQVLLEGQRASSRVWALHRDHGGGRNPEGMRRLLLALVRDLRVVTIVLARQL
                     SRMRAAWRLPAEEQRALAQLTRDIHAPLANRLGIWQLKWELEDLAFRVLEAETYVRIA
                     RLLDDNRSGRERFIQSALGQLRGVLAENGIAADIAGRPKHIYSIWKKMRKKDAPIGEL
                     YDLRALRVLVDDVPTCYAVLGLVHALWVPIPSEFDDYIARPKPNGYRSLHTAVIGPEG
                     KTLEVQIRTREMHAQAELGVAAHWRYKEGSAAGGAAMDKRIAWMRKLLEQAADAAKDG
                     EASPLQGMDADLAEERVYALTPNGDVVDLPQGGTVLDFAYQVHSMVGHRCRGAKVDGR
                     IVPLNTQLQSGQRVEILTGKTAEPRRDWLQAGSGFLASSRSRDKVRAWFNKLDRARNL
                     QAGRELLDRDLRRMGLLHADLQPVLGKFNAATVDELLVLVALGDVGPNQVGRALLELE
                     RAAQEAAAPAASTSTSIVPPKSHASAAKASTVTVQGVDNLLAQVARCCQPLPGEAIVG
                     YLTRARGVSVHRPDCAAFLRLAGIQPARVLPVEWGAQRGGQQVAIRIEGIDRKWLLKD
                     ITTLVAQLGLHVASLHAEPVRGGHGQIRVQLQARVPDFGQLGLLLARLESLPGIESAR
                     RA"
     gene            complement(10345..>11140)
                     /locus_tag="PK399_08100"
     CDS             complement(10345..>11140)
                     /locus_tag="PK399_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HPW13119.1"
                     /translation="PALQDDGDAASLAVHAQREVAERLHPQGVRLLLAIALADKLKQA
                     RRQLPVPPKTSLLYAAIESASPRKDGLKDADRLRADLVDGAFAALAADGLLAIREADA
                     FARRRDAIGKDVFAEAMRRLQQAEAILGLVAEVRAKLESKLIGWARANLDDLQSQLDG
                     LVPPGFLREVPADVLVDYPRYLKAMSLRAERAQRDPVRDQQRMLELAPFVSALATADA
                     THPEAVALRWDLEELRVQLFAQELGAKGGVSPKRLAARLERLQKAG"
BASE COUNT         1582 a         4049 c         3770 g         1639 t
ORIGIN      
        1 acgaccaccg acagcgcatc gccggccacg tccacgccct cccggaagct ggcggtgccc
       61 agcaagacgc cgttgccgga ctcacggaag cgttgcagca acacgtggcg cggctcgctg
      121 ccctgcacga acagcggcca cggcgcgtcg cgcagcaatt ccgcggcctc gcgcagcgca
      181 cggtgcgaag cgaacagcag gaacgcgcgg ccgcccgagg cctgcagcac cggcagcagg
      241 gtcgcgatca gtgcggtgcc gtagtcgcgc gaggccggct ccggcagccg cgtcggcagg
      301 aagcacagcg cctggcggtc ccagtcgaac gggctgggtt cgagcagttc gacggcttcg
      361 ttgatgccca gccgctgggc aatgtgggag aacccaccgg acaccgccag cgtcgccgag
      421 gtgaacaccc aggccgcgcg cgattcctcg cgatgccggc gcaacggcgc ggacacgtcc
      481 agcggcgtgc gctgcaggcg gaagccgcgg ggcgtgagtt cgtaccagtg cacgctggtg
      541 tcgggcggcc tgtcgtcggc atcaaaagtg gggtcagagt cgcctttcgt ttcgaaaggc
      601 gactctgacc ccactttttc gcccagccag cggcgcaggc ggcccagctg gtcgcgcgcg
      661 cgcaggtggc aggcatcgaa gcccggcgcg gcctcgcgca gcggttccaa cgtgtcctgc
      721 atgcgctgca aggcatcggc cagcgactgg aaggcgggtt cgatgtcgac ctcgtccagc
      781 gcgcgccagg ccgtgccgcg caccggcagg ttgtccatgc tcgcgcgcaa cgcacgcgcg
      841 gcctgttcca gtgcgcgcgc cggctcctgc acgctggcca gcgaacccgc gacgtccttg
      901 cattcgccga gtgcgtcgcg cgccagttcc accagcgggc gcgcgcccag gccttcgccg
      961 aagaactgcg cggcgagttc cggcaactgg tgggcctcgt cgaccacgaa cgcctgcgca
     1021 cccggcagga tctcgccgaa gccttcctgc ttgagcgcga ggtcggccag cagcaggtgg
     1081 tggttgacca ccaccaggtc ggcggtctgc gcgcgctggc gcgcctgcac cacgaagcaa
     1141 tcgccccacc gcgggcaatc ggtgcccagg cagttgtcgg cggtcgaact gacctgcggc
     1201 agcagcggcg aatcttccgg gagcgattcc agctcggcca ggtcgcccat cttcgtgcgt
     1261 ccgctccacg cgaccacgcg ctggaactgc gagacctgct cgcgcgacga gaatccaccc
     1321 ttgaacagct gcggatcgcc cttggcctgc tccatccggt acaggcacag gtagttcgaa
     1381 cggcccttca gcaacgcggt cttgaggccg gtgcccaagg catcgcacac ccgcggcagg
     1441 tcgcggaaat acagctggtc ctgcaacgca cgggtgcccg tgctgacgat ggtcttcatg
     1501 cccgacagca gcgcgggcac gaggtaggcg aaggtcttgc cggtgccggt gccggcctcg
     1561 gccaagagca cgccgcgctg ctcgaaggca tccgccaccg ccatcgccaa ccgctgctgc
     1621 gcgggccgcg cgacgaagcc tgggatccgc tctgcaagcg gcgaaccggc acccagcgcc
     1681 tcgccgaccg cctggctcag gcgcgacatc gcgatgcctc ctggatcagg gctgcgctgc
     1741 cggcgtggcc ggtggcgcgt ccgcgggcac cggcacctgc ttggccggat cgtcgccgcc
     1801 gaaccacccg cggatcgcct gccccgcgcg ttccagcgcg ccgggctcgc catcgagcgc
     1861 gggatcggcg gggatcgggt tgccgtattc gtccagcgcc ggcggtggcg cgtacacgca
     1921 ggcctggtag ccgggcgcgt agccggcgac gaaggcgaag cggcgcgcgc cgggacagcc
     1981 cgggtcggtg ctgtggccgc cctcgatcca gcgccagtcc aggccttcat cgctcaattg
     2041 cagcggtgcg ctgggcagcc gcgcgaacac gtccgaccac acccgcatgc cgccggtggc
     2101 gccgtacaaa ccggtttcct tgttctggtc gttgccgacc cagaccacgg cgagatgatc
     2161 gccggtgtaa ccggcgaacc agctgtcgcg accatcgttg ctggtgccgg tcttgccggc
     2221 ggcggccagt cggcccaagc catcacgcac cagcgggcgg ccggtgccgg aactgactgc
     2281 gtactgcagg gccacgccta ccagccgcgc cgcgaccgcg tcgcccttct gcgccggcgg
     2341 cgccacgctg tcgtagcgct tgattgcgcg gccgttcgca tccagcacgc ctcgcaccgc
     2401 atgcagcggc tggatctcgc caccggaggc gaggaactgg tacagctgcg ccatcgcgta
     2461 cgggctctgg tcgaccgcgc ccaggatcag tgacggctgt ccttcggatt cgatcccggc
     2521 cagggtgcgg gtcaactccg ccagccgctg gggctgcacg tccatgccca ggcgcaccgt
     2581 ggcctggttg tagctctggg ccagtgcatc gagcaggcgc acggtgccgt ggctgcgacc
     2641 atcggaattg cccggtttcc agttccgcga acgtcccagg ctgacggtga ccggggcatc
     2701 gctgatagtg ctggcgagcg accacttgtc cggttgcgcc agcgccagca ggtacacgaa
     2761 aggcttcagc aacgagccga ccgggcgctt ggcatccact gcgcggttga agccctgctc
     2821 ggtgaattcg cggctgccga ccaccgccac cacgtcgccg ttgtgcacgt cggtcatgac
     2881 cacgccagcc tgcagcggcg gacgaccctt ggtcgacagc gatttcagcg tgcgcgccac
     2941 cgcgccctcg gcatagccct gcgccgctgg cgacatcccg gtcatcaccg acagcccggc
     3001 accggacagt ttgtccgccg ggtagtcgcg cgccagctgc ttgcgcacca ggtcgacata
     3061 ggcggggaag cggttggcgg ccaggctgcc ggcgttctcg gtcacgccca gcggcgcctt
     3121 ggtcgcccgc tggtattcct tgtcgtcgat caggccggtt tcatgcatct cgccgaggac
     3181 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgctggta ttccttgtcg
     3301 tcgatcaggc cggtttcatg catctcgccg aggacgaaat tgcggcgttc ggtcgcgcgt
     3361 cccgggttgc gccgcgggtt gtaccaggac ggccccttga ccaagccgat catcagcgcg
     3421 atctgttccg tcgaaagatc ctgcagctcc ttgccgaacc agaattcgga cgccgccgcg
     3481 acgccgcgga tcgattgcgc accgcgctgc cccaggtcga cctggttgaa atacgcctcc
     3541 aggatggtgc ccttgtcgta acgcgcttcc agcagcaggg cgaagaggat ttccttgacc
     3601 ttgcgggtga ccgtctgctc cttgccgatg ccgagcaggc cgctgcgcgc aagctgctgg
     3661 gtcagcgtgc tcgccccctg cttggtgtcg ccgcccgact tcacggtgac gaaggccgcg
     3721 cgcagcatgc cgccgagatc gatgcccatg tggcggttga agtcgcggtc ttcgaccgcc
     3781 tgcaggcctt cgaccaggag cgccgggacg tcgccaatgc gcaccagtcg gcgttcctcc
     3841 tggttctggc cgtacaaggt ggccacgcgc gccgggtcca gccgcgcgct gcgcagcgcg
     3901 cgcttgccgg cgacatcgcg aatcgccgcg acgtcgccgc cggacaatgt cacttccagg
     3961 atggccggcg cgacccggcc atcgacatcg tcgtagcctc ggctggaaat ccgccagcgc
     4021 gcgccgttgc ggtcgtaggt gccggcgcgc ctgccatcgc catcacggta agaggcggca
     4081 tccaactcgg tcttcagggt ctgcgcgtcc atcgccagcc cggtgcgcag caccagcgga
     4141 cgcgcgtaca cgcgggtcgg cacctgccag cgcagcttgc cgaagcgctc gccgacctgg
     4201 tggttcaggt acagcatgta cgggatcagg aagccgagca gcaacgccgc ggcagcaagc
     4261 ccccaggtca gcaggcggtt gcgccagacg gggccttgcg aatcggtgcc gtcgccatcg
     4321 tcgtcgtgca ggtcgtcttc gtcttcgatg cggggcatgg gcggcttggc tggcggcggt
     4381 ttcgcgggat gcgacaatgc cgggtacggt tccggcgagt ctagcgcagg gtttgcgcgg
     4441 cccgccgtca gccaacgcct cgttcttcag ccggagttcc gccgcatgcc cctccccgcc
     4501 gaagcgcgct tctggatcgc gcgcgcgctc ggcctgtggc ggcgcggctg ggccagcctg
     4561 cgcacgcgtg ggctggcggc aagctgggcg ggactgttgc ggcaggtccg tcgcaggccc
     4621 cttcccgcgc acgcgctgta cgcgccgccc gcgaccgcgt tttcgccctt cgtgatgccg
     4681 acgtcggcca cgccgcgcgc cagcatcgtg atcccggtgt acggggcctt cgcgcacacg
     4741 ctcgcctgcc tgcgcgcaat cgctgcgcat ccgccggcgg cggcgttcga ggtgatcgtg
     4801 gtcgacgacg cctcgcccga cgattcattg ccgaagctgc gcgcgatcga cggcctccat
     4861 ctgcatgcgc gcgccaccaa cggcggcttc atcgccgcct gcaaggacgg cgcgtcgctg
     4921 gcgcgcggcg aggtgctggt cttcctcaac aacgacaccg tgccgcagcc gggctggctg
     4981 gacgcgctgc tcgccacctt cgagacgcat ccggctgccg gcctggtcgg tgcgaagctg
     5041 ctgtaccccg acggacgcct gcaggaagcc ggcggcgtgg tattcgccga tggcagcggc
     5101 tggaactacg gtcgcttcga ggatcccgcc gactgccgct acacctacct gcgcgatgcc
     5161 gactatgtca gcggcgcggc gatcgcgatc ccacgcgatc tgttcgcgcg gcttggcggc
     5221 ttcgacgcgc gttacgcccc cgcgtattac gaggacaccg acctcgcctt caaggtgcgc
     5281 gccgccggcc ggcgcgtgct ctaccagcct gcatcggtgg tcgtgcatga cgaaggcacg
     5341 acctccggca ccgatctcgg taccggcccc aaggcggcgc aggtccgcaa ccaggccgtg
     5401 ttcgccgaga cgtggcgcca tgcgctcacc gcgcagttgc ccgccggcag cgtgccctcg
     5461 cccgccctgc tgcatcgccg gcaacggcag gtgctggtca ttgatgcgct caccccgcaa
     5521 ccggaccgcg attccggatc gctgcggctg gtcaacctga tgcgcttgct gatcgaggaa
     5581 ggcgcgcacg tggtgttcgt gccggccaac cgcagtgccg atggcgagta caccgcgacg
     5641 ctgcaacaga tgggcgtcga atgctggcat gcgccgacca tgccgcgctt cccggcatgg
     5701 ctgcgcgaac acggcccgcg cttcgacgcg gcgatggtca gccgccacta cgtcgccagc
     5761 gagatgctgc cgctgctgcg caagcacgcg ccgcaagcga aactgctgtt cgataccgtc
     5821 gacctgcact acctgcgcga gaagcgcgca gcggaactga ccgccgatgc cactgcactc
     5881 cgcgctgcga tgcgcacccg cgagctggaa ctggcgctga tccgcgacgc cgacgccacg
     5941 ctcgtcgtca gtcgggtcga gcgcgagttg ctcggcatcg acgcgcccgg tgcgaacgtg
     6001 cacgtactgt ccaacctgca tcgggcagca gcagccgggc cgtcatgggc gctgcgcgac
     6061 ggcctgctgt tcgtcggcgg attccgccat ccgccgaatg tcgatgcggt gctgtggttc
     6121 gttcgggagg tgttcccacg tgttcgcgcg gcacggccca cgatcgaatt ccactgcgtc
     6181 ggtggcgacg tgccgaacga gatcgcgcgc ctcgccgacg tgcccggcgt gcgcgtccat
     6241 ggccacgtgc cggacctgca gccgctgctg gccgcggcgc gcgtgtcgat cgcgccactg
     6301 cgctacggcg ccggcgtgaa aggcaaggtc aaccaggcaa tggcgcatgg cctgccggtg
     6361 gtggccacga cctgcgcggt ggaaggcatg cacctgcgtg acggcgagga cgtgctggtg
     6421 gccgatgacg cgagcgaatt cgccgatgcg gtcctgcgcc tgcttgacga cgaggcgtcg
     6481 tggtcgcggc tggcagtgaa cggacgcgcg aatgtcgccg cgcatttctc gctggacgcc
     6541 gcgcgcgcca cggtgcgcac cctgttcgtc gactgaatcc cgcgcagcct cagttcgcgc
     6601 ccgagctcgc ccgctcgagc cgctgctgca gggcgggcaa tccgggcgtg gccgcgtcca
     6661 gcgcacgcgc acgcgccagt gcacgtgccg tcgcctcgag ctcgccggcg cccaggcgtt
     6721 cttcgccgat cgccagccag cgttcggcca ggcgccgttg cgcggcggcc agcgcggcat
     6781 cagcgggcgc cagttgccgc caggcctcca ggcaaacctg cgcacgcccg aggtcgttgt
     6841 cgcgcaaggc ggtcgcgttg cagtcgcgtg ccgccggcag catcgcctcc agggcgccct
     6901 gcaccgccgg atcggccggc gccagggcgc gcgcctcgcg caggcggtcc catgcgctgt
     6961 cgccgggcgg gctcaaccag tcgccgcggt tgcgcgcggc ctccgcctgc gccagcaggt
     7021 cgcgcacctg cgccttcacc cgtggcgacg tgaccacacg tggcagctgc gcggcgccca
     7081 cacgcgcggc ctgcaggcga tgacgggcgg cctgcacctg caccggatca ccctccaggc
     7141 gttcgaccaa cgccaacagt cgggtggtgt cgtcgaaatg gaagtcggcg gtcgccgttg
     7201 cggcgtcgcg gagcaagcca tccaccactg cgccgctgca ctcatccggc acggccgagg
     7261 ccgcgacctc gcgcaaggcc acgcatgcgt cggcggccgc tgcatgctgg ccacgctgca
     7321 aggattggcg gatgcgcgcg ctgcgctgct gggtggcctg ggccaggccg gcgcgcaatt
     7381 cgggcaagtc gacatggccg ggatcgaaac gttcggcggc ctggatgcgc gacgacgcgc
     7441 cggccaggtc gccacgcgcc agcaatcccg gcaccggctg cagcaagtcc gacagcgcat
     7501 cttcgcggcc ttcgaccgcg cgctggttgc gcggctgcag ggccagcacc cgctggtaaa
     7561 gcggcaaagc ggcatcgaca ccatcccgca gctgtcccgc cacatgcgcg gcttccgctt
     7621 cggcgagcag ggcaacgatc ccggcctgcg cagcatcgtc catgcgcagc aaggcttccg
     7681 cggcatcgat gcgcgccttc ggcgcatcca gctcgcgcgc gaggcgcaag gcgatccgcg
     7741 cctgcgcctc gcgaccgccg cgtgcatgtg cctccgcctg cgccaacgcg gccagcgcga
     7801 cgcgcgacag gccttcgcgc gcttccacct ggtccggctg cagcgccagt gccgcttcgt
     7861 acaactcgcg cgcgccgctg ccatcggcca ccgacaggcg acccgcggcc aacgccacgc
     7921 ccgcctgcgt gcgcagcgcc tcggcgcgcg gatccggcca gatccagttt ccgatgcgct
     7981 ggcggaacag cacggacgca agcaatgcca acagcaaaac cgcacacacc atcaggatcg
     8041 cggttcgcca cggcgtcggc aggccaagcc agcgtgcccg ccacgccgcg cgtctggccg
     8101 gcacggaagc gaagcgcttc atgcccggcg ggcggattcg atgccgggca gcgattccag
     8161 ccgtgccagc aacaagccca actggccgaa gtcgggcacc cgcgcctgca gttgcacgcg
     8221 gatctggcca tggccaccgc gtaccggttc ggcatgcagg ctggccacgt gcaggccgag
     8281 ctgcgcgacc agggtggtga tgtccttcag caaccacttg cggtcgatgc cctcgatgcg
     8341 gatcgcgacc tgctggccgc cacgctgcgc gccccattcg accggcagca cgcgcgccgg
     8401 ctggatgccc gcaaggcgca ggaaggccgc gcaatccggg cgatgcacgc tgaccccgcg
     8461 cgcgcgggtg aggtagccca cgatcgcttc gcccggcaac ggttggcagc agcgcgcgac
     8521 ctgcgccagc aggttgtcca cgccctgcac ggtgaccgta ctcgccttgg ctgccgacgc
     8581 atgcgatttc ggcggcacga tcgaggtcga tgtcgacgcg gccggcgcgg ccgcttcctg
     8641 cgcggcacgc tcgagttcca gcaaggcgcg cccgacctgg ttcgggccga catcgcccag
     8701 cgccaccagg accagcaact cgtccaccgt cgccgcgttg aacttgccga gcaccggctg
     8761 caggtcggcg tgcagcaggc ccatccggcg caggtcacga tcgagcaatt cgcgcccggc
     8821 ctgcaggttc cgcgcgcgat cgagcttgtt gaaccaggcg cgcaccttgt cgcgcgaccg
     8881 gctgctggcc aggaagccgg agcccgcctg cagccagtcg cgacgcggct ccgcggtctt
     8941 gccggtgagg atctcgacgc gctggccgct ttgcagctgg gtgttcagcg ggacgatgcg
     9001 gccgtcgacc ttggccccgc ggcaacggtg cccgaccatg ctgtgcacct ggtaggcgaa
     9061 gtcgagcacg gtgccgccct gcggcaggtc gaccacgtcg ccgttcgggg tcagcgcata
     9121 cacgcgttcc tcggccaggt ccgcgtccat gccctgcagc ggcgaggcct cgccgtcctt
     9181 cgcggcgtcg gcggcctgct ccagcagctt gcgcatccac gcgatgcgct tgtccatcgc
     9241 cgcgccgccg gccgcgctgc cttccttgta gcgccagtgc gcggccacgc cgagttcggc
     9301 ctgcgcatgc atctcgcggg tgcggatctg cacttccagg gtcttgcctt cgggcccgat
     9361 caccgcggtg tgcagcgagc gatagccgtt cggcttgggc cgggcgatgt agtcgtcgaa
     9421 ctcgctcggg atgggcaccc acaacgcatg caccaggccg agcacggcgt aacaggtcgg
     9481 cacgtcgtcg accagcacgc gcagggcgcg caggtcgtag agctcgccga tcggcgcatc
     9541 cttcttccgc atcttcttcc agatgctgta gatgtgcttc ggccggccgg cgatgtcggc
     9601 ggcgatgccg ttctcggcca gcaccccgcg caattgcccc agcgccgact ggatgaagcg
     9661 ttcgcggcca ctgcggttgt cgtccagcag gcgggcgatg cgcacgtagg tctcggcctc
     9721 cagcacgcgg aaggccaggt cctccagttc ccacttcaac tgccagatgc ccagccggtt
     9781 ggccagcggt gcgtggatgt cgcgggtcag ctgcgccagc gcgcgttgct cctccgccgg
     9841 cagccgccag gccgcgcgca tccgcgacaa ctgccgcgcc agcacgatgg tgaccacgcg
     9901 caggtcgcgc accagcgcca gcagcagcct gcgcatgccc tcggggttgc ggccgccgcc
     9961 gtggtcgcga tgcagcgccc agacccgact ggatgcgcgt tggccttcca gcagcacctg
    10021 caggcgctcg gggatctcga tcttcgcctc ggcggcaagc ggctcgatgt ccaccagcgc
    10081 gtccagcaac gccgccgcca tcacttcggc atcggcgttc agggtggaca ggatcgcgag
    10141 cgtgtcgagc aacacatcgg cgcggtcgcg catcggcgcg ccggcctcgg cgcggtcgcg
    10201 ccagaacgcc gccaacggcg ccggcaacgc acctgcgggc aggcgcgcca gggcctgggc
    10261 ataaggcgtc gtggtggggg cgacggcgtg catgcgccta ccttagcgag cgcgccatga
    10321 agcggccagc gcccatgcgc gcgttcatcc ggctttctgc aggcgttcca gccgcgccgc
    10381 gaggcgcttg ggcgagaccc cgccctttgc ccccagctcc tgcgcgaaca actgcacgcg
    10441 caactcctcc aggtcccagc gcaaggccac ggcttccgga tgcgtcgcgt cggccgtcgc
    10501 cagcgcggag acaaagggcg ccaattccag catccgctgc tggtcgcgca ccggatcgcg
    10561 ttgcgcgcgt tcggcgcgca gcgacatcgc cttgaggtag cgcggatagt cgaccagcac
    10621 gtcggccggt acctcgcgca ggaaccccgg cggcaccagc ccgtcgagct gcgactgcag
    10681 gtcatccagg ttggcgcgcg cccaaccgat cagcttcgat tccagcttcg cccgtacctc
    10741 ggcgaccagc cccaggatcg cctcggcctg ctgcaggcgg cgcatcgcct cggcgaacac
    10801 gtccttgccg atcgcatcac gacgccgggc gaaggcatcg gcctcgcgga tcgccagcag
    10861 gccatcggcg gcgagcgcgg cgaacgcccc gtccaccagg tcggcgcgca gtcggtcggc
    10921 atccttcaac ccgtccttgc gcggcgatgc ggactcgatc gccgcgtaca gcagcgaggt
    10981 cttcggcggc accggcaact gccggcgcgc ctgcttcagc ttgtccgcca gcgcgatcgc
    11041 cagcagcagg cgcacgccct gcgggtgcag ccgctcggcg acctcgcgct gcgcgtgcac
    11101 cgcgagcgaa gccgcatcgc catcgtcctg caacgcagga
//
LOCUS       DAOOCX010000075        11026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5563_length_11026_cov_9.373986, whole genome shotgun sequence.
ACCESSION   DAOOCX010000075 DAOOCX010000000
VERSION     DAOOCX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 11026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11026
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5563_length_11026_cov_9.373986"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PK399_08105"
     CDS             complement(<1..775)
                     /locus_tag="PK399_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HPW13120.1"
                     /translation="MNDLAKNLLLWVVVAAVLMVVFQSFSPDRAAGAAAPSYSQFLDQ
                     VDDGNVQKVVFGNESRSGISQIEYTLKNGDKVKTNAPFDRDLVNVLREKRIDMVQQEP
                     ASGISLGAILMNFLPVLLIIGFWLFMMRQMQGGGGGKGAMSFGRSRAKLLNEDQTKVN
                     FADVAGCDEAKEEVSELVEFLRDPGRFQKLGGKIPRGVLMVGPPGTGKTLLARAIAGE
                     AKVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKHAPCII"
     gene            complement(829..1491)
                     /gene="rlmE"
                     /locus_tag="PK399_08110"
     CDS             complement(829..1491)
                     /gene="rlmE"
                     /locus_tag="PK399_08110"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HPW13121.1"
                     /translation="MATRSKSSQRWLKEHFSDPYVKKAQAEGLRSRAAYKLEELVDRD
                     RLLKPGMVVVDLGAAPGGWSQWVRQELDQMERGLPETARRGRVIASDILEMPSLAGVD
                     FLHGDFREDAVLSKLLGMLGGEQVDLVLSDMAPNKSGVDAVDQPRAMHLSELAMDFAD
                     GQLRIGGTFLIKLFQGIGFDDYVKELRRRYAKVAIRKPAASRKRSPEVYALAQGKLAQ
                     MK"
     gene            1561..1866
                     /gene="yhbY"
                     /locus_tag="PK399_08115"
     CDS             1561..1866
                     /gene="yhbY"
                     /locus_tag="PK399_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004087669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HPW13122.1"
                     /translation="MPIALTNAQIRFLRGQAHDLKAMLQVGGKGITDALAAEVDGALE
                     HHELIKVKVAAEDREARDAMIAELAERSDAVLVQRIGHVAVLYRPARDKRQIVLPRA"
     gene            1882..2256
                     /locus_tag="PK399_08120"
     CDS             1882..2256
                     /locus_tag="PK399_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HPW13123.1"
                     /translation="MQLNLERPDYTYFLRGADGTGALVNERRLARSFIIAPSAMVEDW
                     PVQDVRAMTPADLDALLALAPALVVLGTGAQQQFPPAAVMAACLQRGIGLEAMTNAAA
                     ARTYSVLAGEGRRVVAGFVFAT"
     gene            2256..2831
                     /locus_tag="PK399_08125"
     CDS             2256..2831
                     /locus_tag="PK399_08125"
                     /inference="COORDINATES: protein motif:HMM:NF019896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HPW13124.1"
                     /translation="MRALGIAAGVCMLAACGAQPEVDRFDAWRHAHREDVDSYEHYLR
                     AAGVAGIVPLHDLLRSGRRWKQCGTAEFVVPPRGAWHAMTATLRVVGDLRGAGIILAP
                     VVASSFRTTAFNRCEGGSARSRHLANNALDFDLGADAGQRLCDWWRRHGKTRGVGLGF
                     YTPRRIHIDTAGFRTWGSDHTGKTSRCVAGR"
     gene            complement(2832..3608)
                     /locus_tag="PK399_08130"
     CDS             complement(2832..3608)
                     /locus_tag="PK399_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPW13125.1"
                     /translation="MRLPHVLVACIAVVLLASCGRSHVVREGGSSRPRSADSPVRVSQ
                     PKYGATVVVQRGQNVYRIATENGLTALDLALWNDIPPPYTIHPGQRLRLYPGAQGSVA
                     ARPATTPPKKPAASTTAPPRTPAPTPVVVPATSKLAWRWPADGNVVGTYAGGDPTRQG
                     IDIAGKAGQPVRAAADGVVVYSGSGLVGYGELVIVKHDDQWLSAYGHNRARLVNEGQL
                     VKAGQQIAEMGRSGASRDMLHFEIRYNGKPVDPQQYLPTR"
     gene            complement(3617..4231)
                     /locus_tag="PK399_08135"
     CDS             complement(3617..4231)
                     /locus_tag="PK399_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HPW13126.1"
                     /translation="MKLFGPMYERTLRWARHRNATWLLAFLSFIEAIFFPIPPEVMLA
                     PMCLSQPRRAFWFATVSLASAMVGAVIGYYLGHYAWQAVQPLLGKLGMLESIEHGIEV
                     VRAKSVESPWAVFWFLVLAGFSPIPMKVFTWASGIVGVPMLPFMLSMLVGRGKRLYLV
                     AAAIRIGGERAERALHKYIEPIGWSVLVLMAGAAGWFLLRGHAA"
     gene            complement(4228..4926)
                     /locus_tag="PK399_08140"
     CDS             complement(4228..4926)
                     /locus_tag="PK399_08140"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPW13127.1"
                     /translation="MIARMRLQPADIGSGLTGQRVRDRLIDWLRAGSPGYDAITDERV
                     LNAIRTVPRHQFVDEALATRAYENTALPIGHGQTISQPWVVARMTEVLLQQGMPKRVL
                     EIGTGSGYQAAVLAALGIEVYTVERIGDLLRTARKRFRTLGLQVRSKHDDGRIGWPEE
                     APFDGVIVTAAAASLVDALSAQLAPGGTLVAPVGGSSAQSLLRLRKTVDGDIEQDDLG
                     PVVFVPLLSGVADR"
     gene            complement(4923..5711)
                     /gene="surE"
                     /locus_tag="PK399_08145"
     CDS             complement(4923..5711)
                     /gene="surE"
                     /locus_tag="PK399_08145"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019305385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPW13128.1"
                     /translation="MRVLVSNDDGVDAPGIHALAQGLRDAGHEVIVVAPDRDRSGASN
                     SLTLDAPIRVVQLDAHRWKVYGTPTDCVHVALTGMLEALGLEPDIVVSGINNTANLGD
                     DVIYSGTVAAAMEGRFLGLPAVAMSLVTADHKGRHYETAAKAAVEIIARLRTDPLPAN
                     TILNVNVPDLPWGEVRGFETGRLGNRHRAEACTPQQDPRGRQWWWIGAAGIEQDAGPG
                     TDFHAVRTGHISITPIHVDLTRYQALEQVASWVGGLSTTLEPAK"
     gene            5799..6353
                     /locus_tag="PK399_08150"
     CDS             5799..6353
                     /locus_tag="PK399_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPW13129.1"
                     /translation="MRKAPPEIDDRDDAELFRAAIGADRGKVRALPEAPLPPSPPKPK
                     PSAKMARRDEDAARNEFRHAVIAELEAGDVLSHRRDEIPPLVLKRLARGEYSPQEELD
                     LHGLPAHVAEALLREFLRDCRTHGVGCVRIVHGKGRNSEERLPVLKNLVDRVLRHRAD
                     VLAFHSAPAAQGGTGAVLALLEKR"
     gene            complement(6350..7381)
                     /gene="truD"
                     /locus_tag="PK399_08155"
     CDS             complement(6350..7381)
                     /gene="truD"
                     /locus_tag="PK399_08155"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HPW13130.1"
                     /translation="MSEVARAHGAPALSARIRATAEDFFVEELPAFDASGAGEHLLLT
                     IEKRGMNTAFAARRIAAWAGVDESAIGYAGMKDRHAVTRQRFTVWLPKKVAPDFEALQ
                     SDDLRVLDHAWHARKLPRGALAGNRFVLVLREVEGEREVVEARLRTIAAQGVPNYFGE
                     QRFGRGGNNVQQAVAMFAGRRVKREERSMLLSAARSELFNRVLAVRVEAGTWNVALDG
                     EVWMLDGSRSVFGPEAFTDALQARLDAFDIHPTGPLWGEGDLRSSGIARDVELAAIGG
                     DSATRLRNGLERAGLKQERRALRMRPQAFAWDWREDAALELRFELPPGCYATTVLHEL
                     GEVADAAAV"
     gene            complement(7378..7863)
                     /gene="ispF"
                     /locus_tag="PK399_08160"
     CDS             complement(7378..7863)
                     /gene="ispF"
                     /locus_tag="PK399_08160"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPW13131.1"
                     /translation="MNIDFRIGQGFDVHAFGDGDHVMLGGVRVPHARGVLAHSDGDVV
                     LHALCDAILGALALGDIGKHFPPSDARWKGADSRAFVRHCDALIREHGWKVGNCDLTV
                     VCERPKVGPHAMAMRETIAADLGIDIDRVSVKATTTETLGFTGRGEGIAAQAVCLLVR
                     A"
     gene            complement(7860..8549)
                     /gene="ispD"
                     /locus_tag="PK399_08165"
     CDS             complement(7860..8549)
                     /gene="ispD"
                     /locus_tag="PK399_08165"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPW13132.1"
                     /translation="MSWALVPAAGSGRRFGGEVPKQYLHAAGKPLLEHALEALLSHPG
                     VEGVVVALASDDARWPGWTSLQGKPVIACIGGGERADSVLAALHALPTRVAGDELLLV
                     HDAARPNLQADDIGKLIDAATACTDGAILAAPVRDTLKRAGNDKRIARTESRDGLWRA
                     LTPQAFRRDLLLRALQSAEDDGVVATDEAMAVERLGLHPALVEGREDNLKVTTPADLA
                     LAEFLLGRASR"
     gene            complement(8546..8875)
                     /gene="ftsB"
                     /locus_tag="PK399_08170"
     CDS             complement(8546..8875)
                     /gene="ftsB"
                     /locus_tag="PK399_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HPW13133.1"
                     /translation="MKPVRVLLLLLALLLAGLQYTLWLGSGGHQEVAGLRRQVASQQA
                     ENLRLQERNDALAAEVEDLKSGEAAVEERARSELGMVKPGETFYRVVEDAPAGVAAPA
                     PAPAASP"
     gene            complement(8890..10197)
                     /gene="eno"
                     /locus_tag="PK399_08175"
     CDS             complement(8890..10197)
                     /gene="eno"
                     /locus_tag="PK399_08175"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPW13134.1"
                     /translation="MTTTITSVHAREILDSRGNPTLEAEITLADGSFGRALVPSGAST
                     GTKEAVELRDGDKTRYLGKGVLKAVGNVNGAIADMLKGFDAQDQQGLDRRLIDLDGTE
                     NKGRLGANALLAVSMANAHAVAASKKLPLWRHLASISEHPAGWQASLPVPMMNIINGG
                     AHADNNVDLQEFMVLPVGFDSFSEALRSGTEIFHALKSVLKGRGLSTAVGDEGGFAPD
                     LRSNEEALETILEAIGKAGYKAGEDVLLGLDCAASEFFENGKYNLTGEGKRLTSEQFV
                     DFLANWAAQYPIVSIEDGLAEDDWAGWKLLTERLAGKVQLVGDDLFVTNPRIFKDGID
                     QGVANAILVKVNQIGTLSETLEAIAMAERANYAAVISHRSGETEDTTIADIAVATTAT
                     QIKTGSLCRSDRVAKYNQLLRIEQQLGSEARYAGRAAFVSLKR"
     gene            complement(10214..>11026)
                     /gene="kdsA"
                     /locus_tag="PK399_08180"
     CDS             complement(10214..>11026)
                     /gene="kdsA"
                     /locus_tag="PK399_08180"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPW13135.1"
                     /translation="VGLDRPLFLIAGPCVIESEQLQLDVAGQLKEITARLGINFIFKS
                     SFDKANRTSIGSFRGPGMEEGLRVLAEVKRQLGVPVLTDVHEYTPMDEVASVVDVLQT
                     PAFLVRQTDFITKVCATGKPVNIKKGQFLSPWDMKPVVEKAKSTGNSNIMVCERGASF
                     GYNNLVSDMRSLAVMRDTGCPVVFDATHSVQLPGGQGSSSGGQREFVPVLARAAVAVG
                     VAGVFMETHPDPSKALSDGPNAWPLGKMEALLETMLELDAITKRAGFAESTL"
BASE COUNT         1709 a         3859 c         3682 g         1776 t
ORIGIN      
        1 agatgatgca cggcgcgtgc ttcttggcct gctcgaacat gtcgcgcacg cggctggcgc
       61 cgacgccgac gaacatctcg acgaagtcgg agccggaaat gctgaagaac ggcaccttgg
      121 cctcgccggc gatggcgcgg gccagcaggg tcttgccggt gccgggcgga ccgaccatca
      181 acacgccgcg cgggatcttg ccgcccagct tctggaagcg gcccggatcg cgcaggaact
      241 cgaccagctc cgagacctct tccttcgctt cgtcgcaacc ggcaacgtcg gcgaaattga
      301 ccttggtctg gtcctcgttg agcagcttgg cgcgcgagcg gccgaacgac atcgcgccct
      361 tgccgccgcc accgccctgc atctgccgca tcatgaacag ccagaagccg atgatcagca
      421 gcaccggcag gaagttcatg aggatggcgc ccagcgagat gccgctggcc ggctcctgct
      481 ggaccatgtc gatgcgcttc tcgcgcagga cgttgaccag gtcgcggtcg aacggcgcgt
      541 tggtcttcac cttgtcgccg ttcttcaggg tgtactcgat ctgcgaaatg ccggagcggc
      601 tttcgtttcc gaagaccacc ttctgcacgt tgccgtcatc gacctggtcg aggaactgcg
      661 agtaggaggg cgcggcggca cccgctgcgc ggtccggcga gaagctctgg aagaccacca
      721 tcagcacggc agccaccacg acccagagca gcaggttctt cgcaagatcg ttcatgtgtt
      781 cgcttgtcct tcctcgatgc tgccttggaa cgccgggcca aagcccacct atttcatttg
      841 cgccagcttg ccctgcgcca gggcgtagac ctcgggcgag cgcttgcgcg aggccgccgg
      901 cttgcggatc gccaccttgg cataacgccg ccgcagctcc ttcacgtagt cgtcgaaccc
      961 gatcccctgg aacagcttga tcaggaacgt gccgccgatc cgcaactggc cgtcggcgaa
     1021 gtccatcgcc agttcggaca ggtgcatcgc gcgcggctgg tcgaccgcat ccacaccgct
     1081 tttattgggg gccatgtcgg acaacacaag gtcgacctgt tccccgccca gcatccccaa
     1141 cagcttcgat aggacggcat cctccctgaa gtcgccatga aggaaatcga ccccggccag
     1201 gctcggcatc tccaggatgt cgctggcgat gacccgacca cgccgggccg tctccggcag
     1261 gccgcgttcc atctggtcca gttcctggcg tacccattgc gaccagcctc cgggggcagc
     1321 gcccaggtcg accacgacca tgcccggctt gagcaggcgg tcgcgatcga ccagctcctc
     1381 gagcttgtag gccgcacgcg agcgcaggcc ctcggcctgc gccttcttca cgtaggggtc
     1441 ggagaaatgt tccttgagcc agcgctggct ggatttgctg cgagtcgcca cgaccgccgg
     1501 ccagatgggg aaacaggccg ccatgatacc ctgccgcctg tttccgccct ttcgtgccgc
     1561 atgcccatcg ccctcaccaa cgcccagatc cgtttcctgc gcggccaggc gcacgacctg
     1621 aaggcgatgc tgcaggtcgg cggcaagggc atcaccgacg ccctggcggc cgaggtcgat
     1681 ggcgcgctgg aacaccacga gctcatcaag gtgaaggtgg ccgcggaaga ccgcgaggcg
     1741 cgcgacgcca tgatcgccga gcttgcggag cgcagcgacg ccgtgctggt gcagcggatc
     1801 ggccatgtcg ccgtgctgta ccgccccgcc cgggacaagc ggcagatcgt gctgccgcgc
     1861 gcgtaagcca cgcccggcgc gatgcagctc aacctcgaac gccccgatta cacctacttc
     1921 ctgcgtggtg ccgacggcac cggcgcgctg gtcaacgagc gccgcctggc ccgcagcttc
     1981 atcatcgcgc ccagtgcgat ggtcgaagac tggcccgtgc aggacgtgcg ggcgatgacc
     2041 ccggccgatc tcgacgccct gctcgcgctg gcgccggcac tggtcgtgct gggcaccggc
     2101 gcgcagcagc agttcccgcc ggccgcggtc atggccgcct gcctgcagcg cgggatcgga
     2161 ctggaagcga tgaccaatgc cgccgcggcg cgaacctatt cggtgctggc gggcgaagga
     2221 cgccgggtgg tggcggggtt cgtgttcgcg acgtgatgcg tgcgctcggg attgcggccg
     2281 gcgtttgcat gctggccgca tgcggcgcac aaccggaggt cgaccgcttc gacgcctggc
     2341 ggcacgccca tcgcgaggat gtcgattcgt acgagcacta cctgcgcgcg gcaggcgtcg
     2401 ccgggatcgt gccgttgcac gacttgctgc ggtccgggcg tcgatggaaa cagtgcggca
     2461 ccgctgaatt cgtggtcccg ccacgcgggg catggcacgc gatgaccgcc acgcttcgcg
     2521 tcgtcggtga tttgcgcggc gcgggcatca tcctggcacc ggtcgtggcc tcgtcgttcc
     2581 gcacaaccgc tttcaatcgt tgcgaaggcg gcagcgcgag gagccggcac cttgcgaaca
     2641 atgcactcga tttcgacttg ggcgccgatg cggggcaacg cctgtgcgac tggtggcgca
     2701 ggcacgggaa aacccgcggc gtcgggctcg gcttctatac gccgagacgc atccacatcg
     2761 ataccgcggg gttccggacg tggggtagcg accacacagg gaagacatcg cgctgcgtcg
     2821 ccggccgttg atcaacgcgt cggcaggtac tgctgcggat cgaccggctt gccgttgtag
     2881 cgaatctcga agtgcagcat gtcgcgcgat gcgccgctgc ggcccatctc ggcgatttgc
     2941 tggccggcct tcaccagctg gccctcattg accaggcgcg cgcggttgtg gccataggcg
     3001 gacagccact ggtcgtcgtg cttgacgatc accagctcgc cgtagccgac cagtccggag
     3061 ccggaataca ccaccacccc atcggcggcg gcacgcacgg gctgcccggc cttgccggcg
     3121 atgtcgatgc cctgtcgggt cggatcgccc ccggcatagg tgccgaccac gttgccgtcc
     3181 gctggccagc gccacgccag cttgctggtt gccggcacca cgaccggggt gggtgcgggc
     3241 gtgcgaggag gcgccgtggt cgatgctgcg ggcttcttcg gcggcgttgt cgccggccgc
     3301 gcagccacac tgccctgcgc acccgggtac aggcgcaggc gctgtccagg atggatcgtg
     3361 taaggcggcg gtatgtcgtt ccacagcgcc aggtcgagcg cggtcaggcc attctcggtg
     3421 gcgatgcgat agacgttctg gccgcgctgc accaccaccg tcgcaccata tttgggttgc
     3481 gacacacgaa ccgggctgtc ggcgctgcga ggacgcgacg acccgccttc gcgcaccacg
     3541 tggctgcgcc cgcaactggc gagcaggacg acggcgatgc aggcgacgag gacatgaggc
     3601 aggcgcatgg accgcatcat gccgcatggc cccgcaacag gaaccaaccc gcggcgccgg
     3661 ccatcagcac cagcacgctc cagccgatcg gttcgatgta cttgtgcagg gcgcgctcgg
     3721 cgcgttcgcc gccgatgcgg atcgccgcgg cgacgaggta caggcgcttg ccgcgaccga
     3781 ccagcatgct gagcatgaac ggcaacatcg gcaccccgac gatgccgctg gcccaggtga
     3841 acaccttcat cgggatcggc gagaagccgg ccagcaccag gaaccagaac accgcccacg
     3901 gcgattccac gctcttcgca cgcaccacct cgatgccgtg ctcgatcgat tccagcatgc
     3961 ccagcttgcc cagcagcggc tgcaccgcct gccaggcgta atgcccgagg taatagccga
     4021 tcaccgcacc gaccatcgcg ctggccaggc tgacggtggc gaaccagaac gcccggcgcg
     4081 gctgcgacag gcacatcggc gccagcatga cttccggcgg gatcgggaag aagatcgcct
     4141 cgatgaaact caggaatgcg agcagccacg tcgcattgcg atggcgtgcc cagcgcaggg
     4201 tgcgttcgta catcggcccg aacagtttca tcggtcggcc actcccgaca gcagcggcac
     4261 gaagacgacg gggcccaggt cgtcctgttc gatgtcgcca tcgaccgtct tgcgcaggcg
     4321 cagcaaggac tgtgcgctgg aaccaccaac cggtgcgacc agcgtgccac ccggtgcgag
     4381 ctgcgcgctc aaggcatcca ccagcgacgc agcagcagcg gtgacgatga cgccgtcgaa
     4441 cggcgcctcc tccggccagc cgatgcggcc atcgtcatgc ttgctgcgca cctgcaggcc
     4501 caacgtgcgg aagcgcttgc gcgcggtgcg cagcaggtcg ccgatgcgtt cgacggtata
     4561 gacctcgatg ccaagcgcag cgagcacggc ggcctggtag ccggaacccg ttccgatctc
     4621 cagcacccgc ttgggcatgc cctgctgcaa cagcacttcg gtcatgcgcg cgaccaccca
     4681 cggctgcgag atcgtctgcc catggccgat cggcagcgcg gtgttctcgt aggcacgggt
     4741 ggccagcgcc tcgtcgacga actggtggcg cggcaccgtg cggatcgcgt tgagcacgcg
     4801 ctcgtcggtg atcgcgtcgt agcccggcga accggcacgc agccagtcga tgaggcggtc
     4861 gcgcacgcgc tggccggtga ggccactgcc gatgtcggcc ggctgcaggc gcatccttgc
     4921 aatcacttcg cgggctccag cgtggtcgag aggccaccga cccagctcgc cacctgttcc
     4981 agcgcctggt agcgggtcag gtcgacatgg atcggggtga tcgagatatg gccggtgcgc
     5041 accgcatgga aatcggtgcc gggcccggcg tcctgctcga tgcccgcggc cccgatccac
     5101 caccactggc ggccgcgcgg atcctgctgc ggcgtgcagg cctcggcgcg atggcgattg
     5161 ccgaggcgac cggtctcgaa gccgcggacc tcgccccacg gcaggtccgg cacgttgacg
     5221 ttgaggatgg tattggctgg cagcgggtcg gtgcgcaggc gcgcgatgat ctccaccgcc
     5281 gcctttgccg cggtctcgta gtggcggccc ttgtggtcgg cggtcaccag cgacatcgcc
     5341 actgcgggca accccaggaa acggccttcc atcgccgccg cgaccgtccc ggaatagatc
     5401 acgtcgtcac cgaggttggc ggtgttgttg atgccggaga ccacgatgtc cggctccagg
     5461 ccgagcgcct ccagcatgcc ggtcagcgcc acgtgcacgc agtcggtggg ggttccgtac
     5521 accttccacc tgtgcgcatc gagttgcacg acccggatcg gcgcgtccag cgtcagcgag
     5581 ttgctggcgc cactgcggtc gcggtcgggc gcgaccacga tgacctcgtg gccggcatcg
     5641 cgcaggccct gcgcaagcgc gtggatgccg ggggcgtcga cgccatcgtc gttgctgaca
     5701 aggacccgca tggcgctacg acatcgctgg attctgcatg tggccatgat accggatcga
     5761 tcaccggcat cgcccgcgct tcgcggtaac ctgcaccgat gcgcaaggct ccgcccgaga
     5821 tcgacgaccg cgacgacgcc gaactgttcc gcgccgcgat cggtgccgac aggggcaagg
     5881 tccgcgccct gccggaggcg ccgctacctc cttcgccgcc caagccgaag ccatcggcga
     5941 agatggcgcg ccgcgacgag gatgccgcgc gcaatgagtt ccgccacgcg gtgatcgccg
     6001 aactggaagc cggcgacgta ctcagccatc ggcgtgacga gatcccgccg ctggtgctga
     6061 aacggctggc gcgcggcgag tactcgccgc aggaagaact cgacctgcac ggcctgccgg
     6121 cccacgtcgc cgaagctttg ctgcgcgaat tcctgcgcga ctgccgcacc catggcgtgg
     6181 gctgcgtgcg gatcgtgcat ggcaaggggc ggaactcgga agaacggctg ccggtgctga
     6241 agaacctggt cgaccgcgtg ctgcgccatc gcgccgacgt gctcgccttc cactcggccc
     6301 cggcggcgca gggcgggacc ggcgcggtgc tggcgttgct ggagaagcgc tagaccgcgg
     6361 cggcgtcggc gacctcgccc agctcgtgca acaccgtggt cgcatagcaa ccgggcggca
     6421 gctcgaagcg aagttccagc gcagcgtcct cccgccagtc ccacgcgaac gcttgcggcc
     6481 gcatgcgcaa cgcgcgtcgc tcttgcttca ggcccgcgcg ctccagcccg ttgcgcaggc
     6541 gtgtcgcgct gtcgcctccg atcgcggcca gttcaacatc gcgtgcaata ccgctgctgc
     6601 gcaagtcgcc ctcgccccac aacggcccgg tgggatggat gtcgaacgca tcgagccggg
     6661 cttgcagtgc gtcggtgaac gcctccggcc cgaacacgct gcggctgccg tcgagcatcc
     6721 acacctcgcc atcgagcgcg acgttccagg tgccggcctc gacgcggacg gccagcaccc
     6781 ggttgaacag ctccgaacgc gccgcggaca acagcatcga gcgctcctcg cgcttgaccc
     6841 ggcgaccggc gaacatcgcc accgcctgct gcacgttgtt gccgccacgg ccgaagcgtt
     6901 gttcgccgaa gtagttcggc acgccctggg cggcgatcgt ccggagccgc gcttcgacga
     6961 cttcgcgttc gccttcgacc tcgcgcagca ccagcacgaa gcgattgccc gccagcgcgc
     7021 cgcgcggcag cttgcgcgca tgccaggcat ggtcgagcac gcgcaggtcg tcggattgca
     7081 gggcctcgaa gtcgggcgcg accttcttcg gcagccacac ggtgaagcgc tgccgggtca
     7141 ccgcatggcg gtccttcatc cccgcgtacc cgatcgcgga ttcgtccacg cctgcccacg
     7201 ccgcgatgcg cctggccgcg aacgcggtgt tcatgccacg cttctcgatg gtcagcagca
     7261 ggtgttcgcc cgcgccgctg gcgtcgaacg ccggcagttc ctcgacgaag aaatcttccg
     7321 ccgtcgcgcg gatccgcgcc gacagcgcgg gcgcaccgtg cgcgcgcgcg acctcgctca
     7381 tgcgcgcacc agcaggcaca ccgcctgcgc ggcgatgccc tcgccgcggc cggtgaagcc
     7441 gagtgtctcg gtggtcgtgg ccttcacgct gacacggtcg atgtcgatgc cgaggtccgc
     7501 cgcgatcgtt tcgcgcatcg ccatggcatg cggaccgacc ttgggtcgct cgcagaccac
     7561 ggtgaggtcg cagttgccga ccttccaacc gtgctcacgg atgagtgcat cgcaatggcg
     7621 gacaaaggca cgactgtccg cgcctttcca gcgcgcgtcg gaaggcggga aatgtttgcc
     7681 gatgtcgccc agcgccagcg caccgaggat ggcgtcgcag agtgcgtgca ggacgacgtc
     7741 gccatcgctg tgcgccagca ccccgcgcgc atgcggcacg cgcacgccgc cgagcatcac
     7801 gtggtcgccg tcgccgaagg catgcacgtc gaagccctgg ccgatgcgga agtcgatgtt
     7861 catcgcgatg cgcgccccaa caggaattcc gccagcgcca ggtcggcggg cgtggtgact
     7921 ttcaggttgt cctcgcggcc ttccaccagc gcgggatgca ggcccaggcg ttcgaccgcc
     7981 atcgcttcat cggtcgccac gacgccgtcg tcctcggccg actgcagcgc gcgcagcaac
     8041 aggtcgcgac ggaacgcctg cggggtcaat gcgcgccaca ggccatcgcg cgattccgtc
     8101 cgcgcgatgc gtttgtcgtt gcccgcacgc ttcaaggtgt cgcgcaccgg tgcggccagg
     8161 atcgcgccat cggtgcaggc cgtcgcggcg tcgatcagct tgccgatgtc atcggcctgc
     8221 aggttggggc gcgcggcgtc gtggaccaac agcagttcgt ctccagccac gcgggtcggc
     8281 aatgcgtgca atgccgccag caccgaatct gcgcgctcac cgccgccgat gcacgcgatg
     8341 acgggcttgc cctgcagcga cgtccagccc ggccagcgtg cgtcatcgga ggcaagcgcg
     8401 accaccacgc cctcgacccc cggatgcgac agcagggcct ccagcgcgtg ttcgagcaac
     8461 ggttttcccg cggcgtgaag atattgcttg ggcacctcgc caccgaagcg gcggccgctg
     8521 ccggcggccg gcaccagcgc ccagctcacg gactggcagc cggtgccggt gcaggcgcgg
     8581 caacaccggc cggcgcgtcc tcgaccacgc gatagaacgt ctcgcccggc ttgaccatgc
     8641 ccagctcgct gcgcgcgcgc tcctcgaccg cggcctcgcc ggacttgagg tcttcgactt
     8701 cagccgcgag cgcgtcgttg cgttcctgca ggcgcaggtt ttccgcctgc tgcgaggcca
     8761 cctggcgacg caggccggcc acttcctggt gaccgccgct gcccagccag agcgtgtact
     8821 gcaggccggc cagcagcaat gccagcaaca gcagcagcac gcgcaccggc ttcacctccg
     8881 cgttgcctct cagcgcttca gcgagacgaa ggcggcgcgg ccggcatagc gggcctcgct
     8941 gcccaactgc tgctcgatgc gcagcagctg gttgtacttg gccacgcgat cgctgcggca
     9001 cagcgagccg gtcttgatct gggtcgcggt ggtcgccacc gcgatatcgg cgatggtggt
     9061 gtcttcggtt tcgccggaac ggtgcgagat caccgccgcg tagttcgccc gctcggccat
     9121 cgcgatcgct tccagcgttt cgctcagcgt gccgatctgg ttgaccttga ccaggatcgc
     9181 gttggccacg ccctggtcga tgccgtcctt gaagatgcgc gggttggtga cgaacaggtc
     9241 gtcgccgacc agctgcacct tgccggccag gcgctcggtc agcagcttcc agccggccca
     9301 gtcgtcctcg gccaggccgt cctcgatcga gacgatcggg tactgcgcgg cccagttcgc
     9361 gaggaagtcg acgaactgct ccgaggtcag gcgcttgcct tcgccggtga ggttgtactt
     9421 gccgttctcg aagaactcgc tggccgcgca gtccaggccc agcagcacgt cctcgcccgc
     9481 cttgtagccg gccttgccaa tcgcttccag gatcgtttcc agcgcttcct cgttgctgcg
     9541 caggtcgggc gcgaaaccgc cttcgtcgcc gaccgccgtg ctcagtccgc ggcccttcag
     9601 caccgacttc agcgcgtgga agatctcggt gccgctgcgc agcgcttccg agaacgaatc
     9661 gaagcccacc ggcagcacca tgaattcctg caggtcgacg ttgttgtcgg catgcgcgcc
     9721 gccgttgatg atgttcatca tcggcaccgg caggctggcc tgccaaccgg ccggatgctc
     9781 gctgatcgat gccaggtggc gccacagcgg cagcttcttc gaggccgcga ccgcgtgcgc
     9841 attcgccatc gacaccgcca gcagcgcgtt ggcgccgagc cggcccttgt tctcggtgcc
     9901 gtcgaggtcg atcaggcgac ggtccaggcc ttgctggtcc tgcgcatcga agcccttgag
     9961 catgtcggcg atcgcgccgt tgacgttgcc gacagccttg agcacgccct tgccgaggta
    10021 gcgggtcttg tcgccatcac gcagttcgac cgcttccttg gtgccggtgg aggcgcccga
    10081 gggcaccagc gcgcggccga agctgccgtc ggccagggtg atctccgctt cgagcgtggg
    10141 attgccgcgg ctgtcgagga tttcgcgggc gtggacggac gtgatcgtgg tggtcatcga
    10201 gggggtcgtc ggctcagagg gtggattcgg cgaagccggc gcgcttggtg atcgcgtcga
    10261 gctccagcat ggtttcaagc agggcttcca tcttgccgag cggccatgca ttcgggccgt
    10321 cggacagcgc cttgctcgga tcgggatggg tttccatgaa cacgccggct acgccgacgg
    10381 cgaccgcggc ccgcgccagc accggcacga attcgcgctg gccgccgctg ctgctgccct
    10441 gcccgcccgg caactgcacg gaatgggtgg catcgaacac caccgggcag ccggtgtcgc
    10501 gcatcaccgc gaggctgcgc atgtcggaca ccaggttgtt gtagccgaag ctggcgccgc
    10561 gctcgcagac catgatgttg ctgttgccgg tggacttggc tttctcgacg accggcttca
    10621 tgtcccacgg cgacaggaac tggcccttct tgatgttgac cggcttgccg gtggcgcaaa
    10681 ccttggtgat gaagtcggtc tggcggacca ggaaggccgg cgtctgcagc acgtcgacga
    10741 ccgacgccac ttcgtccatc ggcgtgtatt cgtgcacgtc ggtgagcacc ggcacgccga
    10801 gctggcgctt gacctcggcc agcacgcgca ggccctcctc catgcccggg ccgcggaagc
    10861 tgccgatcga ggtgcggttg gccttgtcga agctcgactt gaagatgaag ttgatgccga
    10921 gccgcgcggt gatttccttc agctggccgg ccacgtcaag ctgcaactgc tcgctttcga
    10981 tgacgcaggg cccggcgatc aggaacagcg gccggtccag gccgac
//
LOCUS       DAOOCX010000076        10987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5583_length_10987_cov_7.609587, whole genome shotgun sequence.
ACCESSION   DAOOCX010000076 DAOOCX010000000
VERSION     DAOOCX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5583_length_10987_cov_7.609587"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PK399_08185"
     CDS             complement(<1..240)
                     /locus_tag="PK399_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005420872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPW13136.1"
                     /translation="MHMPYNILFLCTGNSARSILAEGLMNHLSQGRFRAFSAGSQPSG
                     RVNPLALETLETLGCDATGMSSKSWDVFAAPDAPKM"
     gene            complement(265..753)
                     /locus_tag="PK399_08190"
     CDS             complement(265..753)
                     /locus_tag="PK399_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HPW13137.1"
                     /translation="MKRFHVHLNVADLAGSIRFYRELFGAEPSVLKPDYAKWMLEDPR
                     INFAISATGRPPGIDHLGLQVDSGEELVALGQQLEAAGGTLVPEHATVCCYARSDKTW
                     TEDPQGTRWETFHTLAEATTYHAGDAACATDGASCTPDVRAMQPLAENTASCCAPRSG
                     CC"
     gene            complement(750..1091)
                     /locus_tag="PK399_08195"
     CDS             complement(750..1091)
                     /locus_tag="PK399_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPW13138.1"
                     /translation="MDTDTALSALRALGHESRLAAFRQLVQAGPEGLSVGELREHLDL
                     PPATLSAHLNVLRAAGLVGDEREGRVIRVRADYASMDALIAYLTENCCAGAANCGPAS
                     NCTPRQPGARR"
     gene            1380..1967
                     /locus_tag="PK399_08200"
     CDS             1380..1967
                     /locus_tag="PK399_08200"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HPW13139.1"
                     /translation="METDFVPFDWFRIFVGEQPPAYLLEVALKCLLVFCLLLLVMRLS
                     GKRAQNSLSPMQQMLLIALGSAAGDVLLYPSVALSYAALVLVGITLLTVGFEAWSQRS
                     RRVGDFAESRPTVMVEDGVIDHEALRRERTTLRELNAELRVAGARSLSQVRYAILEMT
                     GEFSVFLNDEEPAGEDLLIDAIRLQRGKPTRQAGG"
     gene            2175..2780
                     /locus_tag="PK399_08205"
     CDS             2175..2780
                     /locus_tag="PK399_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13140.1"
                     /translation="MARKIDNAQVTELLLQAIETERGGIKVYTAAIRAAVNEDLRKEW
                     KEYLEETRTHEAILTRVFGELGLDTEAQSPGRAVVAHQAASLVAAIEMAMATAEPAAA
                     ELVAAECVVLAETKDHQNWELIGKVAEQGGPHAKVLKGAHDAVEKDEDHHLYHTMGWC
                     RELWIQSLGMPAVLPPPEEIKQVETAIGASRAEHARQQYLS"
     gene            2850..5372
                     /gene="ligD"
                     /locus_tag="PK399_08210"
     CDS             2850..5372
                     /gene="ligD"
                     /locus_tag="PK399_08210"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HPW13141.1"
                     /translation="MALDEYARKRRFDTTPEPSGHTASGRKGGRPIFVVQLHHASHRH
                     YDVRLQVGNVLKSWAVPKGPSLDPTVKRMAVEVEDHPLDYAGFEGDIPKGHYGAGHVA
                     LFDRGTWTSAGDVAAQLAKGHLRFELQGERLKGGWHLVRSGKPSRQPQWLLFKQDDAW
                     ARAIEADDLLEGVTAAPVAAARRARRPGMHASPGAVATKQSSQRKTRTAALDWAAEAA
                     LLSGARKRAMPVRLPAPQLARLVSSPPPGEHWLHELKWDGYRLLGAIRGGKATLWSRN
                     GLDWTNRVPEVRDALAMLGVRDALVDGELVAGRGSREDFNLLQQVLSGERQGQLRCML
                     FDLLHVDGVDLTSVALVERKALLERLLRHALPMLAYSSHGIGDAGSAFQAAVDAGFEG
                     IVSKRLDGASHPGRGDDWRKCKAQASAEYAVVGYTPPKGSRSGIGSLLLATPDAEHGW
                     RYVGRVGSGFDDAQLEALGQALHGKGGNRPTVHVPENGTDLRQAKWLPMPAFVVEVFE
                     RGTGGRGLLRQASFKALRPDKQVPDLRDDDKAAGETTMKPRKPRKISRVAPALTSPDK
                     VLFPGEGITKRQLADYYAAVMDWLLPGIRQRPLSLVRCPAGIGAQCFFQKHATPGLEL
                     VSRVPVTESDGGSEHYLVVTDAASVMELVQFNSIEFHPWGATTDDIERCDRLVFDLDP
                     DVAVAWPEVVAAARQLRGYLRQAGLESFVRTSGGKGLHLVVPLSPAVPWHQARDFAQA
                     VADAAREAEPLRYVATASKRLRKGKIFIDWLRNGRGATSVASFSARARPGAPVAMPLR
                     WGELGRVAGGDAYDIRNTPARLKRLKSHPWHGMEALRQSLPE"
     gene            5438..6331
                     /locus_tag="PK399_08215"
     CDS             5438..6331
                     /locus_tag="PK399_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HPW13142.1"
                     /translation="MARPIWTGNLSFGLLNVPVSLMSGTRSTDLSFRMLDARDRKPIR
                     FERVNADTGEEVPWKDIVKAFEYDKGSYVVIEKEDIASAAPETHESVEIEAFVDADAI
                     DVRYFEKPYVLVPGKKAEKGYVLLRETLLKARKIGVARVVIRTREYLAAVMPQGDALV
                     LMMMRYPQELVDPSEYKLPQGKAADYRVTARELAMATQLVESMATQWQPDDYHDEFRA
                     RLSEIIRKRIKQKGKTTTVLDEGDEPREDAATNVVDFVALLQKSLGNKGGKAAATGKA
                     AAKKASKTTKTTKSAKPRKTG"
     gene            complement(6453..7835)
                     /gene="dbpA"
                     /locus_tag="PK399_08220"
     CDS             complement(6453..7835)
                     /gene="dbpA"
                     /locus_tag="PK399_08220"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HPW13143.1"
                     /translation="MTTPITFADLALDPSLKAGLDALDYTTLTPIQAQALPAVLEGRD
                     LIAQAPTGSGKTAAFGLGLLQGVDATATRTQALVLCPTRELADQVGKQLRKLATGIAN
                     LKLSVLCGGIPLGPQLASLTHAPHVVVGTPGRVLELVQKEALDLRKVRTLVLDEADRM
                     LDMGFDEPIRALVKRTPKERQTLLFSATFPDSIRTLAKAMLREPLEVSVDGGAQPAAI
                     RAHFFEADAARRAPQLAALLLQFKPESTVVFCNMRRDTEEVVGSLAHYGFTALALHGD
                     MEQRDRDEVLVRFANRSCSVLVASDVAARGLDIDDIGAVVNYELPTDADTYLHRVGRT
                     GRAGRDGLALSLVAPGERNRAALIEERQGAPIRYERVAPLEGQPRDVPQAAMATLRID
                     AGRTDKLRPGDIVGALTGDAGLPKEAIGKIDVFATRSYVAIARTHAKHALAQLRAGKI
                     KGRRMRVNLL"
     gene            7922..9352
                     /locus_tag="PK399_08225"
     CDS             7922..9352
                     /locus_tag="PK399_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPW13144.1"
                     /translation="MQSTPSQDSPSTDLVGQSLWASLRDAIRGTNADYTRIPLRRAVF
                     LLAVPMVLELVLESTFAVVDIFFVAKLGASAVATVGLTESYLFLLYSLAMGLAMAVTA
                     VIARRVGEGKQEEAAVTAVQAIIVALLVSLLPAIIGIAYARELLQLMGADAWAIEHGF
                     RYTQWMLGGNAVIMLLFVINAIFRGAGDAAIAMRVLWVANGLNILLDPLLIFGWGPVP
                     AMGIEGAAIATNIGRGVGVSMQLWILFRGGQHIRVLGSQLAWRGAVLWNIVRTSLGGV
                     GQMLVAMTSWIFLMRILASIGSEAVAGATIAIRVMMFTMMPAWGMSNAAATLVGQNLG
                     AQQAERAEASVWQIGWYNMAYLVLVAVLFFLFPRAILGIFSAGPNVIAIGAEWLRILS
                     YSLFVYGWWMVSVQAFNGAGDTTTPTWINVVFFWLIQIPLAWYLALHLDLRETGVFWA
                     VFVSETSVGLFTLWLFSRGRWKAAQV"
     gene            9416..10513
                     /locus_tag="PK399_08230"
     CDS             9416..10513
                     /locus_tag="PK399_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 domain-containing protein"
                     /protein_id="HPW13145.1"
                     /translation="MTPATRRHRIPALLGLCIGIAGLTACEPQRTPEQVHAELLRKLP
                     ATLHDREGWARDIQTAFTSQDLDPNTSNLCAVLAVVEQESTYRADPPVPGLGGIAIAE
                     IKRRAAAKHVPEFAIDAALKLRGPDGRTYAERLQALRSEREMSELFEQMVARLPLGSG
                     RLLAGLNPVRTGGPMQVRVDFAERHARGYPYTVEGSIRHEVFSRRGGLYFGIKHLLDY
                     PADYDRPLYRFADFNAGRHASRNAAFQQAAAIASGRKLVLDGDLLTPGASMQHPGATE
                     AALRSIDMLLGMDAAAIRADLKRGNQAGFDQTALYRAVFAIADRKGSGKPVPRARVPD
                     IALASPKITRKLTTAWFAGRVESRWNACMAR"
     gene            complement(10523..>10987)
                     /locus_tag="PK399_08235"
     CDS             complement(10523..>10987)
                     /locus_tag="PK399_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13146.1"
                     /translation="MGGMFIYLFFGIHMLMFGLSGFVMAYSSDAPDLTFLYMHGGIAI
                     TVYIVFYLVIFGRDQVGWMLVNAALGILGIHAQIGWILARFDKRIDDYPWQVHVIPFL
                     YYILYTFLLRQFLLDVTRSRSDPRRKRLVDAAYVVFSLLVYGVMLWRLQQAA"
BASE COUNT         1796 a         3529 c         3883 g         1779 t
ORIGIN      
        1 catcttcggc gcatcgggcg cggcgaacac atcccagctc ttgctggaca tgccggtcgc
       61 atcgcagccg agcgtctcga gggtttcaag cgccagcgga ttgacccggc ccgaaggctg
      121 gctgccggcg ctgaaggcac ggaagcggcc ttgggagaga tggttcatca atccttcggc
      181 caggatgctg cgcgcgctgt tgccggtgca gaggaacagg atgttgtacg gcatatgcat
      241 ggggatgtcg tcgtggggtg ggtgtcagca gcagccggat cgcggggcgc agcaggaggc
      301 ggtgttttcg gccagtggct gcatcgcgcg gacgtcgggg gtgcacgacg cgccatcggt
      361 cgcgcaggcg gcatcgcccg cgtgatacgt cgtggcctcg gcgagggtgt ggaaggtttc
      421 ccagcgggtg ccctgcggat cttccgtcca ggtcttgtcg gagcgcgcgt agcagcagac
      481 ggtggcgtgt tccggcacca gggtgccccc ggcggcttcc aactgctgcc ccagcgcgac
      541 gagttcctcg ccgctgtcga cctgcaggcc caggtgatcg atgccggggg ggcggccggt
      601 ggcggagatc gcgaagttga tgcggggatc ctcgagcatc catttcgcgt agtcgggctt
      661 gagcacgctt ggctcggcgc cgaacagctc gcggtagaag cggatgctgc cggcaaggtc
      721 ggcgacattg agatgcacat ggaagcgctt catcgcctgg ctcctggctg gcggggtgtg
      781 caattggagg ccggcccgca attcgcggca ccggcgcagc agttctcggt gaggtaggcg
      841 atcagtgcat ccatgctggc gtagtccgcg cgcacgcgga tcacccgtcc ttcgcgttcg
      901 tcgccgacca ggccggcggc gcgcagcacg ttgaggtgcg cactcagggt ggccggcggc
      961 aaatccaggt gctcacgcag ttcgccgacc gacaggcctt cggggccggc ctgcaccagt
     1021 tgccggaacg cggcaaggcg ggattcgtgg ccgagcgcac gaagtgcgga aagggcggtg
     1081 tcggtgtcaa tgattcgata attccagaag tatcgaataa ttgcaaacgg tcatggctgg
     1141 acgctgccag gggcatgcgc caatgcgccg ttcgaggcgc aatggcgtga gagcgcaggc
     1201 gtgggctggc ccgaccggcg ccggcgatcc aacgggtgct tgcgatgccg ggcgaggtgc
     1261 cgcgcttcgc ccgacccgga gatccatgtc cccatcatgc cgatgtgccg cgatcccttc
     1321 accgcgagct ggcgcgccag tcggcaacaa tgccggccac gccaggtcgg agcttgtcga
     1381 tggaaaccga tttcgtcccg tttgactggt ttcgcatctt cgtcggcgag caaccgccgg
     1441 catacctgct cgaagtcgcg ctgaaatgcc tcctggtctt ctgcctcctg ctgctggtca
     1501 tgcgactgag cgggaaacgt gcgcagaaca gcctctcgcc catgcagcaa atgctgctga
     1561 tcgcgctggg ttctgccgca ggggacgtgc tgctgtaccc gtcggtagcg ctgtcgtacg
     1621 cggcgcttgt gttggtcggg atcaccttgc tgacggtcgg cttcgaggca tggagccagc
     1681 ggtcgcgccg cgtcggcgat ttcgccgagt cccgcccgac ggtgatggtg gaggacgggg
     1741 tgatcgatca cgaggcgctg cgcagggagc ggacgacgct gcgcgagttg aatgcggagt
     1801 tgcgggtggc gggcgcgcgc tcgttgtcac aggtgcgcta cgccatcctc gagatgacgg
     1861 gtgagttcag cgtattcctc aacgacgagg agccagccgg cgaagacctg ctcatcgacg
     1921 cgattcgcct ccagcggggc aagcccacgc gccaggccgg cgggtaacgc ggggcctcgt
     1981 ggcgccagca gtcgggggca tgctcaatcg ttgcacccgg aatggcagcg cttggcaccc
     2041 gcatggagct ggccgggcga ggcgctcgtt gtggcgcatc gtgaatgcgt cgcgggcacc
     2101 ttcgatccac tttcgtcacg cgtccttttc gcctgcctgc gctaaaaacg acgctccccc
     2161 aaggaggtat ccccatggcc aggaagatcg ataacgcgca agtcaccgag ctgttgctgc
     2221 aggcaatcga aaccgaacgt ggcggcatca aggtctacac cgccgcgatc cgtgcggcgg
     2281 tgaatgaaga cctgcgcaag gaatggaagg aatacctgga agagacccgc acgcatgaag
     2341 cgatcctgac gcgggtcttc ggcgagttgg gcctggatac cgaagcgcag agcccgggtc
     2401 gcgcggtcgt ggcccaccag gccgcctcac tggtcgccgc gatcgagatg gcgatggcca
     2461 ctgcggagcc cgccgccgcg gagctggttg ccgcggagtg cgtcgtgctg gccgaaacca
     2521 aggaccacca gaactgggaa ttgatcggca aggtggcaga gcagggaggt ccgcacgcca
     2581 aggtgctgaa aggcgcccac gacgccgtcg aaaaagacga ggaccaccac ctctaccaca
     2641 cgatgggctg gtgccgggag ctgtggatcc agagcctggg catgccggcc gtgctcccgc
     2701 cgccggagga gatcaagcag gtcgaaaccg cgatcggcgc atcgcgtgcc gaacacgccc
     2761 gccagcagta tctgtcctag ccgatcccgg ttggcgctcg cgggtgcccc ggttcccctg
     2821 gcgcgccatg tagggctggg ccgctgacca tggcgctcga cgagtacgcg cggaaaaggc
     2881 gcttcgacac gactcccgaa ccgtccggcc acacggcgag cgggcggaag ggtgggcgac
     2941 cgatcttcgt ggtccagttg caccacgcca gccatcgcca ctacgacgtc aggctgcagg
     3001 tcgggaatgt gctgaagagc tgggccgtgc cgaaggggcc cagcctcgat ccaaccgtca
     3061 agcggatggc ggtggaagtc gaggaccatc cgcttgacta tgccggcttc gagggagaca
     3121 tccccaaggg ccattacggc gctggccatg tcgccctgtt cgatcgtggc acatggacca
     3181 gcgcaggcga cgtggcggcg caactggcca agggccacct tcgcttcgag ttgcagggcg
     3241 agcggctcaa gggcggttgg cacctggtgc ggtcgggcaa gccgtcgcga caaccgcagt
     3301 ggctgctgtt caagcaggac gatgcctggg cgcgagcgat cgaggccgac gacctcctgg
     3361 aaggcgtcac cgccgcgccg gttgccgctg cgcgacgcgc acgtcgtccg ggcatgcatg
     3421 cgagcccagg cgcggtagcg acgaagcagt cttcgcagcg caagacgcgc accgcagccc
     3481 tcgactgggc ggccgaggcc gccctgctgt ccggcgcgcg caagcgcgcg atgcctgtgc
     3541 ggcttccagc gccgcaactc gcgcgcctgg tgtcgtcgcc ccctcccggc gagcattggt
     3601 tgcacgagct gaaatgggat gggtatcgcc tgcttggcgc gatccgcggc ggcaaggcaa
     3661 cgctgtggtc ccgcaacggg ctggactgga ccaaccgcgt gccggaagtg cgggatgcgc
     3721 tggccatgct gggcgtgcgg gatgcattgg tggatgggga gctggtggca gggcggggga
     3781 gccgggagga tttcaacctg ctccagcagg tgctctcggg cgaacgccag ggccagctgc
     3841 gctgcatgtt gttcgacctg ctccatgtcg acggcgtcga tctcaccagc gtcgcgctgg
     3901 tcgagcgcaa ggcattgctg gaacgcctgc tccggcatgc gctgccaatg ctggcctaca
     3961 gctcgcacgg cattggcgat gccgggtccg ccttccaggc ggccgtcgat gccggtttcg
     4021 aaggcatcgt gtccaagcgc ctcgatggcg catcgcatcc gggccgcggc gatgactggc
     4081 ggaagtgcaa ggcacaggca agtgcggaat atgccgtggt tggctacacg ccccccaagg
     4141 gcagccgcag cggcatcggc tcgctgctgt tggcgacgcc ggatgccgaa cacggctggc
     4201 gctacgtcgg tcgcgttggc agcggcttcg atgatgcaca gttggaggcg ctggggcagg
     4261 ccttgcacgg gaaaggcggc aacaggccga ccgtgcatgt ccctgaaaac gggactgacc
     4321 tgcgccaggc aaagtggttg cccatgccgg ccttcgtggt ggaagtattc gagcggggga
     4381 ccggcgggcg tgggctgttg cgacaggcca gcttcaaggc cctgaggccc gacaagcagg
     4441 tgcccgacct gcgcgacgac gacaaggcgg ccggggaaac gacgatgaag ccacgcaagc
     4501 ctcgcaagat cagccgggtc gcgccggcgc tgaccagtcc cgacaaggtg ctgttcccgg
     4561 gggagggcat caccaagcgc caactcgcgg actattacgc cgcggtcatg gattggctgc
     4621 tgcccggcat ccgccaacgg ccgctgtcac tcgtacgctg cccggcgggg atcggcgcgc
     4681 agtgcttctt ccagaagcac gccacgcccg gactggaact tgtctcccgg gtgccggtga
     4741 ccgaatcgga tggcggcagc gagcactacc tggtggtgac cgacgcggcc agcgtgatgg
     4801 aactggtgca gttcaacagc atcgagttcc atccgtgggg cgcgacgacc gacgacatcg
     4861 aacgttgcga tcgcctggta ttcgacctgg atcccgacgt ggcggtggca tggccggagg
     4921 tggtcgccgc cgcgaggcag ttgcgcggtt atctccgcca ggccggcctc gaatccttcg
     4981 tccgcaccag cgggggcaag ggattgcacc tcgtggtgcc gttgtcgccg gccgtgccct
     5041 ggcaccaggc ccgcgatttc gcccaagcgg tcgcggacgc ggcgcgcgag gccgagccac
     5101 tgcgctacgt cgctaccgct tccaagcgac tgcgcaaggg caagatcttc atcgactggc
     5161 tgcgcaacgg gcggggcgcg accagcgtgg ccagcttctc cgcgagggcg cggcccggtg
     5221 cgccggtggc gatgccgttg cgctgggggg agctcggcag ggtcgcaggc ggcgatgcct
     5281 acgatatccg caacacgccg gcccggttga agcgcctcaa gtcgcacccg tggcacggca
     5341 tggaggcatt gcgccagtcg ctgccggaat gaccttgtcc acaagctcgt gacatcgcgc
     5401 ggtgcaggct cgaccttcct ccatcgagtc cgccgccatg gcccgcccca tctggaccgg
     5461 aaacctgtcg ttcggcctgc tgaatgtccc ggtgtcgctg atgtcgggaa cccgcagcac
     5521 ggacctgtcc ttccgcatgc tggacgcacg cgaccgtaag ccgatccgct tcgagcgcgt
     5581 caatgccgac accggcgagg aagtgccctg gaaggacatc gtcaaggctt tcgagtacga
     5641 caagggcagt tatgtggtga tcgagaagga ggacatcgcc tccgccgcgc ccgagaccca
     5701 cgagtccgtc gagatcgagg cgttcgtcga tgccgatgcg atcgatgtcc gctacttcga
     5761 gaagccctac gtgctggtcc ccggcaagaa ggccgaaaag ggctacgtcc tgctgcgcga
     5821 gaccttgctc aaggccagga agatcggcgt ggcgcgcgtg gtcatccgca cccgcgaata
     5881 cctggccgcg gtcatgccgc agggcgacgc gctggtgctg atgatgatgc gctatccgca
     5941 ggaactggtg gacccgtccg aatacaagct cccgcagggc aaggccgcgg actaccgggt
     6001 gaccgccagg gaactggcga tggctacgca actggtggaa tccatggcga cccaatggca
     6061 gccggacgac taccacgacg agttccgtgc ccgcctgtcc gagatcatcc gcaagcgcat
     6121 caagcagaag ggcaagacca ccacggtgct ggacgagggc gacgagccgc gggaggacgc
     6181 cgccaccaac gtggtcgact tcgtggcctt gctgcagaag agcctgggca acaagggtgg
     6241 caaggcggcc gcgaccggga aggccgcggc caagaaggcg tcgaagacga cgaagaccac
     6301 caagtcggcg aagccccgca agacaggttg atccaccggg aaacggcgct tggcaaacag
     6361 ggccacgggc catcgtcgcg cggcaccgct gcgggatgca cggccgggac gcagcgtgcc
     6421 ccgcagggcg tcattggacg gaccccgacg gcctacagca ggttcacccg catccgccgc
     6481 cccttgatct tgcccgcgcg caattgcgcc agcgcgtgct tcgcatgcgt tcgggcgatg
     6541 gcgacgtagc tgcgcgtggc gaacacgtcg atcttgccga tcgcctcctt cggcaaacca
     6601 gcgtcgccgg tcagcgcgcc gacgatgtcg ccggggcgca gcttgtcggt gcggccggcg
     6661 tcgatgcgga gggtggccat cgcggcctgc ggcacgtcgc gtggctggcc ttcgagcggg
     6721 gcaacgcgct cgtagcggat cggcgcaccc tggcgttcct cgatcaatgc agcgcggttg
     6781 cgttcgcccg gcgcgaccag gctcagggcc aagccatcgc ggccggcgcg accggtgcgg
     6841 ccgacccggt gcaggtaagt atcggcatcg gtgggcagtt cgtagttcac caccgcgccg
     6901 atgtcgtcga tgtcgagtcc gcgtgcggcg acgtcgctgg ccaccagcac gctgcagctg
     6961 cggttggcga agcgcaccag cacttcgtcg cggtcgcgct gttccatgtc gccatgcaag
     7021 gccagcgcgg tgaacccgta gtgcgccagc gagccgacga cttcctcggt gtcgcggcgc
     7081 atgttgcaga acaccaccgt cgattccggc ttgaactgca acagcagcgc ggcgagttgc
     7141 ggcgcgcgac gcgcggcatc ggcctcgaag aaatgcgcgc ggatggcggc cggctgcgcg
     7201 ccgccatcga cgctgacttc cagtggctcg cgcagcatcg ccttggccag cgtgcggatg
     7261 ctgtcgggga acgtcgccga gaacagcaac gtctgccgtt ccttgggcgt gcgcttgacc
     7321 agcgcgcgga tcggctcgtc gaagcccatg tcgagcatgc ggtcggcttc gtccagcacc
     7381 agggtgcgca ccttgcgcag gtccagcgcc tccttctgca ccagttccag cacgcggccg
     7441 ggcgtgccga ccaccacgtg cggtgcatgc gtgagcgagg cgagttgcgg ccccagcgga
     7501 atgccgccgc aaagcaccga gagcttgagg ttggcgatgc cggtggccag cttgcgcaac
     7561 tgcttgccga cctggtcggc gagttcgcgg gtagggcaga gcaccaaggc ctgggtgcgg
     7621 gtcgcggtgg catcgacgcc ctgcagcagg cccagcccga atgccgcggt cttgccgctg
     7681 ccggtcggcg cctgcgcgat caggtcccgg ccttcgagca cggccgggag cgcctgcgcc
     7741 tggatcggcg tcagcgtggt gtagtcgagt gcgtcgaggc cggctttcag ggacggatcg
     7801 agggcgaggt cggcgaaggt gatgggcgtg gtcatgcgcg catgatcgca ttcctcgtcg
     7861 tgtcgatcct gcgcccggcc gttcgtcgca gctaacatcc gcccgaacgc aaggaggcac
     7921 catgcagtcg acaccctcgc aggactcgcc ctcgaccgac ctcgtcgggc aatcgctgtg
     7981 ggccagcctg cgcgacgcga tccgcggcac caatgcggac tacaccagga tcccgctgcg
     8041 ccgcgcggtg ttcctgctgg cggtgccgat ggtgctggaa ctggtgctgg agtcgacctt
     8101 cgcggtggtc gatatcttct tcgtggccaa gttgggcgcg tcggcggtgg ccacggtcgg
     8161 gctgaccgag agctacctgt tcctgctgta ttcgctggcg atgggcctgg cgatggcggt
     8221 caccgcggtg atcgcgcggc gcgtcggcga aggcaagcag gaggaggccg cggtgaccgc
     8281 cgtgcaggcg atcatcgtcg cgctgctggt gtcgctgctg ccggcgatca ttggcatcgc
     8341 ctacgcacgc gagctgttgc agctgatggg cgcggatgcg tgggccatcg agcacggctt
     8401 ccgctacacg caatggatgc tcggcggcaa cgcggtgatc atgctgctgt tcgtcatcaa
     8461 cgcgatcttc cgcggtgccg gcgatgccgc catcgcgatg cgcgtgctgt gggtcgccaa
     8521 tggcctgaac atcctgctcg atccgctgct gatcttcggc tggggcccgg tgccggcgat
     8581 gggcatcgaa ggcgcggcga tcgccaccaa catcggccgt ggcgtcggcg tctcgatgca
     8641 gttgtggatc ctgttccgcg gcggccagca catccgggtg ctgggctcgc aactcgcgtg
     8701 gcgcggcgcg gtgctgtgga acatcgtgcg cacctcgctg ggcggtgtcg gccagatgct
     8761 ggtagcgatg acctcgtgga ttttcctgat gcggatcctg gccagcatcg gcagcgaggc
     8821 cgtggccggg gcgaccatcg cgatccgggt gatgatgttc acgatgatgc cggcctgggg
     8881 catgtcgaac gcggcggcga ccctggtcgg tcagaacctc ggcgcgcagc aggccgagcg
     8941 cgccgaggcc tcggtctggc agatcggctg gtacaacatg gcctacctgg tgctggtcgc
     9001 cgtgctgttc ttcctgttcc cgcgggcgat cctcggtatc ttcagcgcgg gcccgaatgt
     9061 aatcgccatc ggcgcggagt ggctgcgaat cctgtcgtat tcgttgttcg tgtacggctg
     9121 gtggatggtc agcgtgcagg cgttcaacgg tgccggcgac accaccacgc cgacctggat
     9181 caacgtggtg ttcttctggc tgatccagat tccgctggcc tggtaccttg ccctgcacct
     9241 ggacctgcgg gagaccgggg tgttttgggc ggtattcgtc tcggagacgt cggtggggct
     9301 gttcaccctg tggctgttct ctcgcgggcg ctggaaagcc gcgcaggtct aacgacactc
     9361 ccgcttcatt cagcgtggcc tgctagcctc tgccgaaccc cgccggcagc gccgcatgac
     9421 gcccgcaact cgacgccatc gaatccccgc gctgctcggc ctgtgcatcg gcatcgccgg
     9481 cctgacggca tgcgaaccac agcgcacgcc ggagcaggtg catgccgagt tgctgcgcaa
     9541 gttgccggcc accctgcatg accgcgaggg ctgggcacgc gatatccaga ccgccttcac
     9601 ctcgcaggac ctcgatccga acacgtcgaa cctgtgcgcc gtgcttgcgg tggtggagca
     9661 ggaatcgacc taccgcgccg atccgccggt acccgggctg ggcggcatcg ccatcgccga
     9721 gatcaagcgc cgcgccgccg cgaagcacgt ccccgaattc gccatcgatg ccgcgttgaa
     9781 attgcgcggg ccggatggcc gcacttacgc ggagcgcctg caagccctgc gcagcgagcg
     9841 cgagatgagc gagttgttcg agcagatggt ggcgcgcctg ccgctgggca gcggcaggct
     9901 gctggcaggc ctgaacccgg tgcgcaccgg cgggccgatg caggtccggg tggatttcgc
     9961 ggagcgccac gcacgcggtt acccgtacac cgtcgagggc tcgatccgcc atgaagtgtt
    10021 cagccgtcgc ggcgggctgt acttcggcat caagcacctg ctggattatc cggccgacta
    10081 cgacaggccg ctctaccgct tcgccgattt caacgcgggc cggcatgcca gccgcaatgc
    10141 cgcgttccag caagcggcgg caatcgccag tggcaggaag ctggtgctcg atggcgacct
    10201 gctcaccccc ggtgcgtcga tgcaacatcc cggggcgacc gaagccgcgt tgcgcagcat
    10261 cgacatgttg ctgggcatgg acgccgccgc gatccgcgcg gacctcaagc gcggcaacca
    10321 ggccggcttc gaccagactg cgttgtaccg tgcggtgttc gccatcgccg atcgcaaggg
    10381 cagcggcaag ccggtgccgc gcgcgcgggt gccggacatc gcgttggcca gtcccaagat
    10441 cacccgcaag ctgaccaccg cgtggttcgc cgggcgggtg gagtcgcgct ggaacgcctg
    10501 catggcgcgc tgacgcaccg gttcacgccg cctgctgcag gcgccacagc atgactccgt
    10561 acaccagcag cgagaacacc acgtaggcgg catcgaccag gcgcttgcgt cgcggatcgc
    10621 tgcggctgcg ggtcacgtcg agcaggaact ggcgcagcag gaaggtatag aggatgtagt
    10681 acaggaacgg gatgacgtgg acctgccacg ggtagtcgtc gatgcgcttg tcgaagcgcg
    10741 ccaggatcca gccgatctgc gcatggatgc cgaggatgcc gagcgcggcg ttgaccagca
    10801 tccagcccac ctggtcgcga ccgaagatca ccaggtagaa cacgatgtac acggtgatgg
    10861 cgataccgcc atgcatgtac aggaaggtca ggtccggcgc gtcggacgaa tacgccatga
    10921 cgaagcctga caggccgaac atcagcatgt ggatgccgaa gaacaggtag atgaacatcc
    10981 cgcccat
//
LOCUS       DAOOCX010000077        10951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5612_length_10951_cov_8.327735, whole genome shotgun sequence.
ACCESSION   DAOOCX010000077 DAOOCX010000000
VERSION     DAOOCX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5612_length_10951_cov_8.327735"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PK399_08240"
     CDS             <1..769
                     /locus_tag="PK399_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW13147.1"
                     /translation="NALRYTDEGGVLVGVRHQHDGRLRIDVWDTGSGIAPEHQAQVFD
                     EFYQVSQCSDETGERKRGLGLGLATVRRLAELVGCEVRLASRLRRGTVVSLRLDAGQV
                     GAGAGDEPADMPLDISGLRVLVVDDEASILEGLRALLQEWGCEIRIADDEASATTAVA
                     GWRQPPDLVISDLRLREGRSGLDVLRTLARHYGADPQRPPFARLLVTGETRPDRIGEI
                     AANRIPVLFKPVSPSRLREAMLATVLASRVMPMKSEE"
     gene            766..2349
                     /locus_tag="PK399_08245"
     CDS             766..2349
                     /locus_tag="PK399_08245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW13148.1"
                     /translation="MSDGFAYDVLEYPSHVHPQAHPSRLAAIGRLHGIDAASPRDCHL
                     LEVGCGDGLQLLTLAMVYPQSRFVGVDLSQAAIARGEATRRRLGLENLQLVAADLTTW
                     DPGLDPFDYIVAHGFFSWVPDVVRDCLLGLCRQALSPAGIAYVSYNALPGCHLRRLMW
                     DMLRHHVRGIDDPRTRIAAARELLASLESDVIGSRVYADVIRSEARDLLRRTDTSVLF
                     HDDLADTNQPFSISEFATRAGEHQLRYLGETDYHEMSDAGLTDDARARLDARAGGDRL
                     QREQYLDYLKGRRFRQTLLCRADAPMQDLADAAMARGLSAVGHLRTDDAEGNALDLAE
                     GVAVRFHSGDGAMLTTDHPVIKAALAMVGNAFPAAPAFDEVLIAARQASQSQNTIEAD
                     ADALAHTWLAAFELGLMILHCDPPGFATEAGATPKANALARMQVEAGSDLITSLRPSM
                     IRIDSPLALELIRLLDGSRDRATLMHDLAERMAALPVDASVGGDHALRDAAWWRAALA
                     GQLEAGLSQTARMGLLEAD"
     gene            complement(2367..2807)
                     /gene="tssE"
                     /locus_tag="PK399_08250"
     CDS             complement(2367..2807)
                     /gene="tssE"
                     /locus_tag="PK399_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HPW13149.1"
                     /translation="MRGLMDRLVDAPLPEDGGRRVSVTDMRNSVSRDLENLLNTRSEG
                     ARLISDSFKECRASSLTYGILDFSSYSLLSPHDRDRIRRSLELAIGLHEGRLTRIRVT
                     LEPQRELDRALRFRVDALMELGPDNEKVQFDAVLQLNTQTYVVR"
     gene            complement(2894..3385)
                     /locus_tag="PK399_08255"
     CDS             complement(2894..3385)
                     /locus_tag="PK399_08255"
                     /inference="COORDINATES: protein motif:HMM:NF017454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HPW13150.1"
                     /translation="MASDYLLELEGIKGESLDEKHKDKIEIDSFSWGVSNDGNMGRGG
                     GGGTGKANFQDVHFTKQTDKSSALLVKSAASGDHIKKAVLTARKAGGKGGQVDYLVIT
                     MEDVMVSSFQTGGNAGNSSIPIDQFALNYTKIKYEYKPQKADGGLEGAIMAQYDRALN
                     KAT"
     gene            complement(3424..4908)
                     /gene="tssC"
                     /locus_tag="PK399_08260"
     CDS             complement(3424..4908)
                     /gene="tssC"
                     /locus_tag="PK399_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011259606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HPW13151.1"
                     /translation="MANTETQAAGAAAAEAADGGLLDQILTQSKIARSDTERTRARDL
                     IAELVSQVSDGAMVVSKDAIGALDARIAEIDRVLSEQLSSVMHAPEFQQLEGSWRGLK
                     YLVDNSETSTNLKIKVFNTTKKELVKDFKSASDFDQSTLFKKLYEEEYGTFGGAPFAT
                     LIGDFEFGRHPEDMYLVEEISHVCAAAHAPFLSAASPELLGFDDFTELSGPRDLAKIF
                     DTVEYAKWKSFRASEDSRYVGLAMPHVLGRLPYGPDTDPVEAFNFVERTDGTDHNKYL
                     WMNAAYALGTKVTDAFAKYGWCAAIRGVEGGGLVEGLPTHTFATDDGEVALKCPTEIA
                     ITDRREKELADLGLIPLVHCKGTDYAAFFSTQSAQKAKEYNTDAANANARLSSQLQYI
                     FAVSRIAHYLKSMMRDKIGSFASKSSVELFLNNWISQYVLLDDNASQEAKAQYPLREA
                     RIEVTEVPGKPGAFKSVAFLRPHYQLDELSVSLRLVAELPKSAR"
     gene            complement(4975..5487)
                     /gene="tssB"
                     /locus_tag="PK399_08265"
     CDS             complement(4975..5487)
                     /gene="tssB"
                     /locus_tag="PK399_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HPW13152.1"
                     /translation="MAKKESTQHKLDRVRAPRVQLTYDVEVGDAIEKKELPFVAGVVG
                     NFTGQPAEPLAKLKDRKFVNVDKDNYDDVLKGMKPRVQMQVDNKLADDGSKLGVELTF
                     RSLEDFSPERVANQIEPLRKLLEARQKLADLRNKAAGNDKFEDLLNDVLQNTDKIAQL
                     SREVGGKSEE"
     gene            complement(5697..6785)
                     /gene="tssA"
                     /locus_tag="PK399_08270"
     CDS             complement(5697..6785)
                     /gene="tssA"
                     /locus_tag="PK399_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HPW13153.1"
                     /translation="MPIDVNELLAPIPGSDPAGSDASFSDQFDRIREARRADDPNLAQ
                     GEWQTELKVADWREAQSLSEDILLRTSKDLQAAVWLAEAAISRDGLEGARDSFSLLAG
                     LLETYWEGLYPRADDGDLEERAGKLAWFATYGARALQAMRLNDDPQAPLTLAGWIDSR
                     EVDNLGRQNAEAYQAALDEGRINGETYDARMVATPEAMLMERLVQVQAARDAFIRFSS
                     TSDAKLGRDAPNLAAIDDGLKKIQQIYAKVATAKGIGGVATVADDGAGEQAAASGGVA
                     TGGASGGTVALDLGGGSLASKQAALRAIGEIAGFFRRTEPHSPVAYLLERAIVWADMP
                     LEQFLAELIRDESTLSSIRERVGLPPSY"
     gene            complement(6807..10403)
                     /locus_tag="PK399_08275"
     CDS             complement(6807..10403)
                     /locus_tag="PK399_08275"
                     /inference="COORDINATES: protein motif:HMM:NF025689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein"
                     /protein_id="HPW13154.1"
                     /translation="MNPLDFVERLFSNIGSVFTRIGYLYEKSSDTAKKPWLRRIVVTL
                     VLLVVVVGLVFLGLALIPMVPMRFWQVLAICLVALVVLWWFTAGQRKASLKGRTRKRI
                     GDLGPGNAEDEKVPTQRMTAAIAEAKRTIARSPEIEKGRNPLYRIPWMLFVGDQPADV
                     LGILRAGSEVSPFPASDKPAMDADDVWRWWFHKSMIAIEMHPRVVADAGARLDRGLWY
                     QALMQLANEREKLPLNGIVVTVAAQTLIGPADKLKETATRLRRLVDEAMEHLQVQMPV
                     YFVVSGLDRLPGYAQVRATLPAEAFSQALGHRFADNEVVSAASSGRIEEVLRPIEERL
                     HALRMTAMRAQHTPQARRAVFEFIEALRQAGQGLNLFVGLMLEDNPFQRTPRWRGLYF
                     AGGADANNRGGAFVADLFTRFLPSDQPLAGTSFKGNAGRMAIAGVGVLAMLGLSASIA
                     YGLSSARRDDTALLAQTRSACAEVADRTSNGRITWVAGCGRTVEALDAAASESLLSFG
                     IRRADADIETLRQRVIDDFNNLILAPEDQMLATDIEQRRAGIEHALAITQRLRLLDDC
                     RDGNDDCRKRELPNNVSFDARSRLFAPFVSPDHDTRRDRDNADALFATYLGYLRWQKK
                     NVLDAERERLEGLLTKLLANYTPRAADLQKWADTRGQGVTLATFWLPEGAVVGTEGDE
                     MPGVSRAYTRDAWEGIVEPLLVTLRAQAPAQQARVDDLRTSYFRDYFASWAKFQSRFQ
                     DGIGLWRGRYGDLQARAASHQNPYALFFQSAQRNLHELPLDWPFSSRWAATWARMKSD
                     WMSSWRPFGRFVADSFKFGGEKVTPPVWLLAMHDTETRVLAPAQADYARAYLRLQAEG
                     SGEDVYQLASDLFASKGKADKPPASDYTAMLDAVDKPGEKFATAFKGNDLGAWSVVQG
                     PARLILFLTVHRAGEYVQERWRDSVVKPLAALPQEQQIEALYGEQGKLGAFVNDWLKP
                     FITEKERLPVKVGGVAMPLTPGYQNMVAAQRKFLPVLDNGPPFLAGSFTLTQPSQLGS
                     LDEGDGGTVFEVECRERVYRASSAGESLADATAQVFWSAGSCLQARIRLSIAAPPVEG
                     AEAVQEFDPATSQMVDAAPAGEPISLTRIYAGPDGFAKLIEEFAGGSRVFAVEDFRES
                     YSPAQWSELRQRLAEAKFRSARVFLKVELSDQMKQYLGASTARAEVPSVILE"
     gene            complement(10400..>10951)
                     /locus_tag="PK399_08280"
     CDS             complement(10400..>10951)
                     /locus_tag="PK399_08280"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPW13155.1"
                     /translation="VKQRISALEGVKSADVQVQLRIWPHCEVVKILEPFEARNKDSGY
                     GLTVTPSTGHSDRFIEGEKVTIKVTQANFDGYLYVDYYFVDGNMAHIFPHPNEPDSGR
                     LVKAGETLVIGNTPEGWEVGAPFGQELITVISSPTPLYDGALPTFDKAEDYLPKLRQM
                     LDANRGNTKLVATYLFMQTEPAR"
BASE COUNT         1682 a         3632 c         3684 g         1953 t
ORIGIN      
        1 caatgcgctg cgctacaccg acgagggcgg cgtgctggtc ggcgtgcgcc accagcacga
       61 tggccgcctg cggatcgacg tctgggatac cggcagcggc atcgcacccg aacaccaggc
      121 gcaggtgttc gacgagttct accaggtcag ccagtgcagc gacgaaaccg gtgaacgcaa
      181 gcgcggcctg ggattgggac tggcgacggt gcgccggctc gccgaattgg tcggttgcga
      241 ggtgcgcctg gcctcgcggt tgcgccgcgg caccgtggtc tcgttgcgtc tggatgcggg
      301 ccaggtcggt gccggggcgg gcgacgaacc ggcggacatg ccgctggaca tcagcggcct
      361 gcgggtgctg gtggtcgacg acgaagccag catcctcgaa ggcctgcgcg cgttgctgca
      421 ggaatggggc tgcgagatcc gcatcgccga cgacgaggcc agcgccacca ccgcggtcgc
      481 cggctggcgg cagccgcccg acctggtgat cagcgacctg cgcctgcgcg agggccgcag
      541 cggcctggac gtgctgcgca cgctggcccg ccactacggt gccgatccac agcgcccgcc
      601 gttcgcgcgc ctgctggtca ccggcgaaac ccgccccgac cggatcggcg aaatcgccgc
      661 caatcgcatc ccggtgctgt tcaagccggt ctccccatcc cggctgcgcg aggcgatgct
      721 ggccaccgtg ctggcgtccc gggtcatgcc catgaaatcc gaagaatgag cgacggcttc
      781 gcctacgacg tcctggaata tccctcgcat gtgcatccgc aggcgcatcc gtcgcggttg
      841 gccgcgatcg gccgactgca cggcatcgat gccgcctcgc cgcgcgactg ccacctgctg
      901 gaagtcggtt gcggcgatgg cctgcaactg ctgaccctgg cgatggtgta tccgcagagc
      961 cgcttcgtcg gcgtggatct gtcgcaggcc gcgatcgccc gcggcgaagc gacacgacgc
     1021 aggctcggcc tcgaaaacct gcagttggtc gcagcggacc tgaccacatg ggatccgggc
     1081 ctcgatccat tcgactatat cgtcgcccac ggtttcttct cgtgggtgcc ggacgtcgtg
     1141 cgcgactgcc tgctcgggct gtgccgacag gcactgtcgc ccgccggcat cgcctatgtc
     1201 agctacaacg ccctgcccgg ctgccacctg cggcgactga tgtgggacat gctgcgccac
     1261 catgtgcgcg gcatcgacga cccgcgcacg cgcatcgcgg cagcgcgcga gttgctggcc
     1321 tcactggaat ccgacgtcat cggctcgcgg gtctatgccg acgtgatccg cagcgaagcc
     1381 cgtgacctgt tgcgccgcac cgatacctcg gtgctgttcc acgacgacct ggccgacacc
     1441 aaccagccgt tttcgatcag cgagttcgca acgcgcgcag gcgagcatca acttcgctat
     1501 ctcggtgaaa ccgactacca cgagatgagc gatgccgggc tgaccgacga tgcccgcgcg
     1561 cgcctggacg cgcgcgccgg cggcgatcgc ctgcaacgcg agcaatacct ggactacctg
     1621 aagggacgcc gcttccggca aaccctgctc tgccgggccg atgcgccgat gcaggacttg
     1681 gcggatgcgg cgatggcgcg cggattgtca gcggtcgggc acctgcgcac cgacgatgcc
     1741 gaaggcaatg cgctggatct cgccgaaggc gtggccgtgc gcttccacag cggcgacggc
     1801 gcgatgctga ccaccgacca cccggtgatc aaggccgccc tggcgatggt cggcaatgcc
     1861 ttcccggcgg cacccgcctt cgatgaggtc ctgatcgccg cgcgacaggc cagccagagc
     1921 cagaacacga tcgaggcgga tgccgatgcg ctcgcccaca cgtggctggc cgcgttcgaa
     1981 ctgggactga tgatcctgca ttgcgatccg cccggcttcg cgaccgaagc cggcgcaaca
     2041 ccgaaggcga atgcgttggc acgcatgcag gtggaggccg gctccgacct catcaccagc
     2101 ctgcgtccgt cgatgatccg catcgacagc ccactcgcgc tggagttgat ccgcttgctc
     2161 gacggcagtc gcgatcgcgc cacactcatg cacgaccttg ccgaacgcat ggcggcattg
     2221 ccggtcgacg cgagtgttgg tggcgaccat gcgttgcgtg atgccgcctg gtggcgcgcg
     2281 gcgctggccg gacaactgga agcggggttg tcgcagaccg cgcggatggg cttgctggaa
     2341 gcggattgac gcgcgggcga tgccgctcaa cgcaccacgt aggtctgggt gttcaattgc
     2401 agtacggcat cgaactgcac tttctcgttg tccggcccca gttccatcaa tgcgtccacc
     2461 cggaagcgca gggcgcgatc gagttcgcgt tgcggctcga gggtgacccg gatgcgggtg
     2521 agacggccct cgtgcaagcc aatcgccagt tccagcgaac gccggatgcg gtcgcgatcg
     2581 tgcgggctga gcaagctgta ggacgagaaa tccaggatgc cgtaagtcaa cgaggacgcc
     2641 cggcattcct tgaagctgtc gctgatcagg cgcgcgcctt cggaacgcgt gttgagcagg
     2701 ttctcgaggt cgcgcgacac gctattgcgc atgtcggtaa cggacacccg ccgcccgccg
     2761 tcttccggca acggcgcatc gaccaggcga tccatcaatc cacgcatgca acaggctccg
     2821 tttcagtcgc gatcccggaa cgaaaacggc ccgccgcgtg gtgcgcggcg ggcctgtttc
     2881 agcaacggac ggatcaggtc gccttgttca gggcgcggtc gtactgcgcc atgatcgcgc
     2941 cttccaggcc gccatcggcc ttctgcggct tgtactcgta cttgatcttc gtgtagttca
     3001 gggcgaactg gtcaatcggg atcgaggaat tgccggcatt gccgccggtc tggaacgagc
     3061 tcaccatcac gtcttccatg gtgatgacca ggtaatcgac ctggccgccc ttgccgccgg
     3121 ccttgcgcgc ggtcaacacc gccttcttga tgtgatcgcc gctggccgcg gacttcacca
     3181 gcagggcgct cgacttgtcg gtctgcttgg tgaaatgcac gtcctggaag ttggccttgc
     3241 cggtgccgcc gccgccgccg cgacccatgt tgccgtcgtt cgacacgccc cagctgaacg
     3301 agtcgatctc gatcttgtcc ttgtgctttt cgtcgagcga ttcgcccttg atgccttcga
     3361 gctcgagcag gtaatcggaa gccatggtgg tatctcctca gaggttggaa agggaatgcg
     3421 gttttagcgg gcggacttcg gcagctcggc gacgaggcgc aacgagacgc tgagttcgtc
     3481 caactggtag tgcgggcgca ggaaggcaac cgacttgaag gcgcccggct tgccggggac
     3541 ttcggtcacc tcgatgcgtg cctcgcgcag cgggtactgc gccttggctt cctgcgaggc
     3601 gttgtcgtcc agcagcacgt actgcgaaat ccagttgttg aggaacagct cgacgctgga
     3661 cttgctggcg aagctgccga tcttgtcgcg catcatcgac ttcaggtaat gcgcgatgcg
     3721 gctgaccgcg aagatgtact gcagctgcga ggacaggcgc gcattggcgt tggccgcgtc
     3781 ggtgttgtac tccttggcct tctgcgccga ctgggtggag aagaacgccg cgtagtcggt
     3841 gcccttgcag tgcaccagcg ggatcaggcc gaggtcggcg agttccttct cgcggcggtc
     3901 ggtgatcgcg atctcggtcg ggcacttcag cgccacttcg ccgtcatcgg tggcgaaggt
     3961 gtgggtcggc aggccttcga ccaggccacc gccctcgacg ccgcggatcg ccgcgcacca
     4021 gccgtacttg gcgaacgcgt cggtgacctt ggtgcccagc gcatacgcgg cgttcatcca
     4081 caggtacttg ttgtggtcgg tgccgtcggt gcgctcgacg aagttgaacg cctcgaccgg
     4141 atcggtgtcc gggccgtacg gcaggcggcc aagcacgtgc ggcatcgcca ggccgacgta
     4201 gcgggagtcc tcggaggcgc ggaagctctt ccacttcgcg tattcgacgg tgtcgaagat
     4261 cttggccagg tcgcgtgggc cggacagctc ggtgaagtcg tcgaagccca gcagctccgg
     4321 cgaggccgcg ctgaggaacg gcgcatgcgc cgcggcgcag acgtgcgaga tttcctcgac
     4381 caggtacatg tcctccggat ggcggccgaa ctcgaagtcg ccgatcaggg tggcgaacgg
     4441 cgcgccgccg aaggtgccgt actcttcttc gtagagcttc ttgaacagcg tgctctggtc
     4501 gaagtcggac gcgctcttga agtccttgac cagttccttc ttggtggtgt tgaagacctt
     4561 gatcttcagg ttggtgctgg tctcggagtt gtcgaccagg tatttcaggc cgcgccagct
     4621 gccttccagc tgctggaatt ccggcgcatg catgaccgag gacagctgct cggaaagcac
     4681 gcggtcgatt tcggcgatgc gcgcgtccag cgcaccgatg gcgtccttgg acaccaccat
     4741 cgcgccgtcg ctgacctgcg aaacgagctc cgcgatcagg tcgcgggcgc gggtgcgctc
     4801 ggtgtcggaa cgggcgatct tgctctgggt gaggatctgg tccagaaggc cgccatcggc
     4861 tgcttctgct gctgcggcgc ccgccgcctg ggtttcggta ttggccatga ggtctctgct
     4921 cgtttggtct gggcgtgtgg atcgaaggca gcgactacgc tgccgacggg gcgcttattc
     4981 ctcggacttg ccgccgactt cgcgggacag ctgcgcgatc ttgtcggtgt tctgcaggac
     5041 gtcgttcagc aggtcctcga acttgtcgtt gccggcggcc ttgttgcgca ggtcggcgag
     5101 cttctgccgc gcctcgagca gcttgcgcag cggttcgatc tggttggcta cgcgctccgg
     5161 cgagaaatcc tccaggctgc ggaaggtcag ttcgacgcca agcttggagc cgtcgtcggc
     5221 cagcttgttg tcgacctgca tctgcacgcg cggcttcatg cccttgagca cgtcgtcgta
     5281 gttgtccttg tcgacgttga cgaacttgcg gtccttcagc tttgccagcg gctcggccgg
     5341 ctggccggtg aagttgccga ccacgcccgc cacgaacggc agttccttct tctcgatcgc
     5401 atcgcccact tcgacgtcgt aggtgagttg cacgcgcggt gcgcgcacgc ggtcgagctt
     5461 gtgttgggtg ctttccttct tggccatgac gttccccgtg cgcgtggtgt tggtgggtgt
     5521 tgctcttgat tggaacggag ctgcgcgcca aaggcggcct ccgctcatca tgcaaacgag
     5581 tctaggaacc gcgaccgccc ggcagaatcc gccggacggc cagaaatggg catatgccat
     5641 tcgacctgag cggcacggtg gccgtcgcgc acccgcgaat gcgtcggtgc cccatgtcag
     5701 taggacggcg gcaagccgac ccgttcgcgg atcgaggaca gggtgctttc gtcgcggatc
     5761 agttccgcca ggaactgttc cagcggcatg tcggcccaga cgatggcgcg ctccagcagg
     5821 taggcgaccg ggctgtgtgg ttcggtgcgg cggaagaaac cggcgatttc cccgatggcg
     5881 cgcaaggccg cctgcttgga ggccaggctg ccaccgccca ggtccagcgc caccgtaccg
     5941 cccgatgccc cgccggtcgc gacaccgcca ctggcggcgg cctgctcgcc tgcgccgtcg
     6001 tcggcgaccg tggccacccc gccgatcccc ttggcggtgg cgaccttggc gtagatctgc
     6061 tggatcttct tcaggccatc gtcgatcgcg gccaggttgg gggcatcgcg ccccagcttg
     6121 gcatcggagg tgctgctgaa gcggatgaag gcatcgcgcg cggcctggac ctggaccagc
     6181 cgctccatca gcatggcttc tggcgttgcg accatccgcg cgtcataggt ttcgccattg
     6241 atgcggcctt cgtccagcgc cgcctggtag gcctccgcgt tctgccggcc caggttgtcg
     6301 acttcgcgcg aatcgatcca gcctgcgagg gtcagcggtg cctgcggatc gtcgttcaac
     6361 cgcatcgcct gcagtgcgcg cgcgccatag gtcgcgaacc aggcgagctt gcccgcgcgt
     6421 tcttccaggt cgccgtcgtc ggcgcgtgga tacaggcctt cccaatacgt ctccagcagg
     6481 cctgccagca gcgagaaact gtcgcgcgcg ccttccaggc cgtcgcgcga gatcgccgct
     6541 tccgccagcc acactgctgc ctgcaggtcc ttgctggtac gcagcaggat gtcctcggac
     6601 aggctctgcg cctcgcgcca gtcggccacc ttgagttcgg tctgccactc gccctgggcc
     6661 aggttgggat cgtccgcgcg gcgggcctcg cggatgcggt cgaactggtc ggagaagctg
     6721 gcatcgctgc ccgccgggtc gctgcccgga atcggggcga ggagttcgtt gacgtcgatc
     6781 ggcatgacag gctccggagg tcgggattat tcgaggatca cgctcgggac ttcggcgcgc
     6841 gcggtgctgg cgccgaggta ctgcttcatc tggtcggaca gttcgacctt caggaacacg
     6901 cgggcgctgc ggaacttggc ttcggccagg cgctggcgca gctcggacca ttgcgccggg
     6961 ctgtagctct cgcggaagtc ctccaccgcg aacacgcgcg agccgccggc gaattcctcg
     7021 atgagcttgg cgaaaccgtc cgggccggcg tagatccggg tcaggctgat cggctcgccg
     7081 gctggcgcgg catcgaccat ctggctggtg gccgggtcga attcctgcac ggcctcggcg
     7141 ccttccaccg gcggcgcggc gatgctgagg cggatccgtg cctgcaggca gctgcccgcc
     7201 gaccagaaca cctgggcggt ggcatccgcc agcgattcgc cggcggaact ggcgcgatac
     7261 acgcgctcgc ggcattcgac ctcgaagacc gtgccaccat cgccttcgtc cagtgagcca
     7321 agctggctgg gctgggtcag ggtaaagctg ccggccagga acggcgggcc attgtcgagc
     7381 accggcagga acttgcgctg cgcggcgacc atgttctggt agcccggcgt cagcggcatc
     7441 gcgacgccgc cgactttcac cgggaggcgt tccttctcgg tgatgaaggg cttgagccag
     7501 tcgttgacga aggcaccgag cttgccctgc tcgccataga gcgcttcgat ctgctgctcc
     7561 tgcggcaacg ccgccagcgg cttgaccacg ctgtcgcgcc agcgctcctg cacgtattcg
     7621 ccggcacgat gcacggtcag gaacaggatc agccttgccg ggccctgcac caccgaccat
     7681 gcccccaggt cgttgccctt gaacgcagtg gcgaacttct cacccggctt gtcgacggcg
     7741 tcaagcatcg ccgtgtagtc cgatgccggg ggcttgtccg ccttgccctt gctggcgaac
     7801 aggtcggacg ccaactggta gacgtcctcg ccggaaccct ccgcctgcag tcgcagatag
     7861 gcgcgcgcgt agtcggcctg cgccggggca aggacccggg tctcggtgtc gtgcatcgcc
     7921 agcagccaca ccggcggcgt caccttctcg ccaccgaact tgaaggagtc cgccacgaac
     7981 cgcccgaacg gccgccacga cgacatccag tcgctcttca tccgcgccca cgtggccgcc
     8041 cagcgcgacg agaacggcca gtcgagcggc agttcatgca ggttgcgctg ggcggactga
     8101 aaaaaaagcg cgtacgggtt ctggtggctg gccgcgcgcg cctgcaggtc gccgtagcga
     8161 cctcgccaca ggccgatgcc gtcctggaag cgcgactgga acttggccca gctggcgaag
     8221 tagtcgcgga agtagctggt gcgcaggtcg tcgacccgtg cctgctgcgc cggcgcctgt
     8281 gcccgcaacg tgaccagcag gggctcgacg atcccttccc aggcatcgcg ggtgtaagcg
     8341 cgggacacgc cgggcatctc gtcgccctcg gtgcccacca ccgcgccttc gggcagccag
     8401 aacgtcgcca gggtgacgcc ctgcccgcgc gtgtcggccc atttctgcag gtcggcggcg
     8461 cgcggtgtgt aattcgccag caatttcgtc agcaggcctt cgagtcgctc acgctctgcg
     8521 tcaaggacgt tttttttttg ccagcgaagg tagccgaggt aggtggcgaa cagggcatcg
     8581 gcgttgtcgc ggtctcgtcg ggtgtcgtgg tccgggctga cgaagggcgc gaacaggcgc
     8641 gagcgcgcgt cgaagctgac gttgttcggc aattcgcgct tgcggcagtc gtcgttgccg
     8701 tcgcggcaat cgtcgagcag gcgcaggcgc tgggtgatcg cgagcgcgtg ttcgatgccg
     8761 gcgcgtcgct gctcgatgtc ggtcgccagc atctggtcct cgggcgcgag gatcaggttg
     8821 ttgaaatcgt cgatcacgcg ttgccgcagg gtctcgatgt cggcatcggc gcgacggatg
     8881 ccgaaactca gcaggctttc gctggcggcg gcgtccaagg cttcgaccgt gcgcccgcat
     8941 ccggccaccc aggtgatccg gccattggag gtgcgatccg ctacctcggc acaggcgctg
     9001 cgcgtctgtg ccagcagcgc agtgtcatcg cggcgcgcgg aggacaagcc ataggcaatg
     9061 gacgccgaca accccagcat cgccagcacg ccgacgccgg caatcgccat ccgcccggca
     9121 ttgcccttga agctggtgcc ggccagcggc tggtcgctgg gcaggaagcg ggtgaacagg
     9181 tcggcgacga aggcaccacc gcggttgttc gcatcggcac caccggcgaa gtacaggcca
     9241 cgccagcgcg gggtgcgctg gaacggattg tcttccagca tcaggccgac gaacaggttg
     9301 agtccctgcc ccgcctggcg cagcgcctcg atgaactcga acaccgcgcg ccgcgcctgc
     9361 ggcgtgtgct gcgcgcgcat ggcggtcatg cgcagcgcat gcaggcgctc ctcgatgggc
     9421 cgcaatactt cctcgatgcg cccgctgctg gccgcgctga ccacttcgtt gtcggcgaag
     9481 cgatggccga gcgcttgcga gaacgcctcg gccggcaggg tggcgcgcac ctgcgcgtaa
     9541 cccggcaggc gatccaggcc gctgaccacg aagtacaccg gcatctgcac ctgcaggtgc
     9601 tccatcgcct cgtccaccag ccggcgcagg cgggtggcgg tttccttgag cttgtcggcc
     9661 gggccgatca gcgtctgcgc ggccaccgtc accacgatgc cgttcaacgg cagcttttcg
     9721 cgctcgttgg ccagttgcat cagcgcctgg taccagaggc cgcgatccag ccgggcgccg
     9781 gcatccgcca ccacgcgcgg gtgcatctcg atggcgatca tcgacttgtg gaaccaccag
     9841 cgccagacat cgtccgcgtc catcgccggc ttgtcggacg cggggaacgg cgagacctcg
     9901 ctgccggcgc gcaggatgcc gagcacgtcc gcgggctggt cgccaacgaa cagcatccac
     9961 gggatccggt agagcggatt gcggcccttc tcgatttccg gtgagcgcgc gatggtgcgc
    10021 ttggcctcgg cgatcgccgc tgtcatccgt tgcgtcggga ctttctcgtc ttccgcgttg
    10081 ccaggaccaa ggtcgccaat gcgcttgcgc gtgcgtccct tcagcgaggc cttgcgctgg
    10141 ccggcggtga accaccacag cacgaccagg gcgaccaggc agatcgccag gacctgccag
    10201 aaccgcatcg gcaccatcgg gatcagggcc aggcccagga acaccaggcc gaccacgacc
    10261 accagcagca ccagggtcac cacgatgcgg cgcagccacg gcttcttggc ggtgtcggaa
    10321 ctcttctcgt acaggtagcc gatccgggtg aacaccgaac cgatgttcga gaacaggcgt
    10381 tccacgaagt ccagcggatt catcgtgcgg gctccgtctg catgaacaag taggtcgcga
    10441 ccagcttggt gttgccgcgg ttggcgtcga gcatctgccg cagcttgggc aggtagtcct
    10501 cggccttgtc gaaggtcggc agtgccccgt cgtacagcgg cgtgggcgaa ctgatcacgg
    10561 tgatcagctc ctggccgaat ggcgcgccca cttcccagcc ttccggcgtg ttgccgatca
    10621 ccagggtctc gccggccttc accaggcgcc cgctgtcagg ctcgttgggg tgcgggaaga
    10681 tatgcgccat gttgccgtcg acgaagtagt agtcgacgta gaggtatccg tcgaaattcg
    10741 cctgggtgac cttgatggtg accttctcgc cctcgatgaa gcggtccgaa tggcccgtgc
    10801 tgggcgtcac cgtgagtcca tagcccgaat ccttgttgcg cgcctcgaac ggctccagga
    10861 tcttcaccac ctcgcaatgc ggccagatcc gcagctgcac ctgcacgtcg gcggacttca
    10921 cgccttcgag cgcgctgatg cgttgcttga c
//
LOCUS       DAOOCX010000078        10926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5631_length_10926_cov_7.966608, whole genome shotgun sequence.
ACCESSION   DAOOCX010000078 DAOOCX010000000
VERSION     DAOOCX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5631_length_10926_cov_7.966608"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PK399_08285"
     CDS             complement(<1..641)
                     /locus_tag="PK399_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HPW13156.1"
                     /translation="MLPYRKPTEGRDYWVFDDVLPNAGDVRERCIGKQDWALGFPHTA
                     ESWPGRRALPALDADEMALVEGKVRQATGMKKLWVANTPDGKTLNHNCVQVVGEREAG
                     PRPHTDSRALCRYAGVLYLTPDAPEHCGTSFYRQRLPTGQLGGNTVNRPHNTLVEAMG
                     TRFVPPDSFVEDVRVPNRFNRLLVYSAAMIHSASAYCGNVLADERMAAVFFWMG"
     gene            736..1485
                     /gene="tpiA"
                     /locus_tag="PK399_08290"
     CDS             736..1485
                     /gene="tpiA"
                     /locus_tag="PK399_08290"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPW13157.1"
                     /translation="MRRKIVAGNWKLHGTRAFATALVAEIAATPVPGVELVILPPLPY
                     IGDLIEDFEGTPLAFGAQDVSSNEQGAYTGEVSASMLVDVGARYGLVGHSERRQYHAE
                     SSELVALKFQAAIHAGLCPILCLGEGLEQREAGQTEAVIAAQLHPVLERVGVAAFARA
                     VVAYEPVWAIGTGRTASPEQAQAVHAFIRGEVAAHDATIAGSLPILYGGSVKPGNAAS
                     LFGQADVDGGLIGGASLVAADFLAIATAAAA"
     gene            1508..1981
                     /gene="secG"
                     /locus_tag="PK399_08295"
     CDS             1508..1981
                     /gene="secG"
                     /locus_tag="PK399_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010375880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPW13158.1"
                     /translation="MWLQLINVVYVLVAISMVVLILMQRGAGAQAGSGFGGGASSTVF
                     GSRGSSNFLTKSTKWLGILFFVIAMGMAMYASKSATGTLDAKPDLGVMGQAAPASAKP
                     APKVPTAPAQPAPSVPAPAIPVAAPQYAQPEAAQPQPATQVPAPAPAPAAPAQGG"
     gene            2025..2109
                     /locus_tag="PK399_08300"
     tRNA            2025..2109
                     /locus_tag="PK399_08300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2059..2061,aa:Leu,seq:gag)
     gene            2217..2645
                     /locus_tag="PK399_08305"
     CDS             2217..2645
                     /locus_tag="PK399_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW13159.1"
                     /translation="MPNLSTILKGEITRLARKEIKAAVDPVRKANAGHRREIAELKRQ
                     VASLQRDLKSSSKPAKVREPEGDASPGSQRFSAKGLKSLRAKLGLSAAEFGQLVGASG
                     QSIYKWEAGKAVPRASQRAALVAMRGIGKREAAKRLASMQ"
     gene            complement(2801..3436)
                     /locus_tag="PK399_08310"
     CDS             complement(2801..3436)
                     /locus_tag="PK399_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPW13160.1"
                     /translation="MSDTRNQMTHDVLARMAGHGDIRYFEKGEILFEEHDAPDGVYIL
                     LEGELKVYVDGRQGREVVYATLKPIELFGEMLLDGGARSASVKALTRARCAVVPADAL
                     RALVRSEPDFAEHTILGLIARLRTLSQSTRRLATHQVRERVFALLDDAAVDEGNVRAI
                     ARPLTQQEIANRVGASREMVNHVLRDLRTAGHIMRDDGDRLLILKPLPAAR"
     gene            3625..4962
                     /locus_tag="PK399_08315"
     CDS             3625..4962
                     /locus_tag="PK399_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW13161.1"
                     /translation="MDEPSTATGNIVARSRGKARQSAGDAERNAMSDLWDIIAAQSEI
                     ANAGLDLDRVVDAITRKAQVLTGSTGAVVEMLHGPDLVYWSGSGSVREHIGVRIPAIG
                     SLSGLCVAKGALLVCTDSESDPRVNIAACRRVGLRSALVTPLHCEGELVGVLKVLSNR
                     ADAYSQRHVAMLETLASFIGSTLNSALEHAKLRDALSRGSHAEPDAKPSLDEERARIS
                     DLIHGGGVSPVFQPIVELASRRIVGFEALSRFAAGMHAPADGWFEAATRVGMCLDLET
                     ACISSIVAAVSRGPQRHGYISLNASPMTLMEYDFGLLPDRSAHGGWVLEITEHSEVDD
                     YTALAGRVQALQRAGFRIAIDDAGAGYASLRHVLRLSPDIVKLDISITRDIDKIVKHQ
                     QLAAAIISFSRDTGIELVAEGIETEAERDTLAALRVPYGQGYLFGKPLPLAYG"
     gene            complement(4997..5434)
                     /locus_tag="PK399_08320"
     CDS             complement(4997..5434)
                     /locus_tag="PK399_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HPW13162.1"
                     /translation="MSDPGVELDLGDPLLAGVFFVTGDDLATLDVAARDAGLLARRID
                     LGDCAGKAALLARIAEVLAMPDGRARNWDALGDSLRDLSWLPAPGYAVLFDGAQHLRD
                     GDEASFDTLLTLLEEASAEWATQEVPFWSFLALPEDEFEGAAP"
     gene            complement(5431..5898)
                     /locus_tag="PK399_08325"
     CDS             complement(5431..5898)
                     /locus_tag="PK399_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HPW13163.1"
                     /translation="MRASRRRNPLAWVLLAIALVVAWKLWPANGEAPAAAHLPTTGPA
                     PVATPSPSGMREAASPALPDFLPAQAHRTVDLIRRGGPFPHRQDGSVFGNREGHLPRQ
                     PRGWYREYTVDTPGLDHRGARRIVTGGQPPREWYYTDDHYDSFRRFEVPGGMP"
     gene            complement(5901..6149)
                     /locus_tag="PK399_08330"
     CDS             complement(5901..6149)
                     /locus_tag="PK399_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13164.1"
                     /translation="MAQTERRHSPHHSCRELRRELNEAQRDTLGELERYGWELKFVRH
                     PLFQPLVPVLYDPDRRGYAILEEDGTLNEHPPFVIRPH"
     gene            6217..6831
                     /locus_tag="PK399_08335"
     CDS             6217..6831
                     /locus_tag="PK399_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPW13165.1"
                     /translation="MPTDPRIDAYIERAAPFARPILVHVRALVHQACPGVEETIKWGM
                     PTFMHAGGILCGMAAFKQHASFGFWKHAMVVGEGERADEKPRDGMGSYGKLTSLDDLP
                     AKKALLAHIHKAMKLNEDGVKVPAARKAAPRPAPETPADLLAAFKQDKAAQATFDAFP
                     PSCKREYIEWIVEAKREDTRAKRLAQAVEWLAEGKRRNWKYENC"
     gene            complement(6876..8654)
                     /locus_tag="PK399_08340"
     CDS             complement(6876..8654)
                     /locus_tag="PK399_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPW13166.1"
                     /translation="MRRALLATALLACPLLMGTSPAFARSVALTTTAERSGFKQTGRY
                     DEVIALCDAFEQAYPQSVRCFTFGTTPEGRPMKAMAVSTSGALDPKAAQARDLPVVLV
                     QGGIHAGEIDGKDAGFWLIRDLLQGKVGAGLLDKAVLLFVPVFNADGHENFRAWNRPN
                     QRGPEQMGYRVTAQRYNLNRDYVKADSPEMQAMLGLVNQWDPLAMLDLHVTDGAKFQH
                     DISITGEPTNSGDIALREAGNALKDGIIAKLATQGSLPVGFYPSFVEDDDPASGFIHG
                     VSTPRFSNGYFPLRNRLAILVETHSWRTYPERVRSTYNTVLDALELTATNGTRWLQLA
                     RDADARAAALGGQSVPVEYKATDKVRTIDFLGYAYTRTPSDISGGTMTRYDETTPQVW
                     KIPLRDEIVPATSVVAPKGGYLVPQSYAVAVARQLLTHGITFRVLTAPLRDPNAQAFV
                     ADAVQFSPTSVEGHQRVTLKGAWTKHSAEIAAGGLYVPIAQAKSRLLVNILEPTAGDA
                     MAAWGDFNNAFERKEYMEGYVAEEQARLMLAQDPALKAEFERKLREDAEFARSPGARL
                     DFFYRRHPAWDAGTNRYPVLRTDTVY"
     gene            8781..9431
                     /locus_tag="PK399_08345"
     CDS             8781..9431
                     /locus_tag="PK399_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HPW13167.1"
                     /translation="MSASNTRMTLYYAPGAASFLVHWLLIDLDAPHALHLVDTAARAQ
                     KSPEYLALNPNGVVPTLVVDGRPHYEAAALAMLLAERHPDAGLAPAFDDPRRADYLQW
                     MFNLANMVQPLFRQWWYPGEPAGEANADAIRAHCAPRIEAEWQRIDDHLAANGPHLLG
                     EGLSVADFYLVMLMRWSRNMPKPATEWPHLAALAQRLKARPSFKALYEREGLAEWA"
     gene            9478..>10926
                     /locus_tag="PK399_08350"
     CDS             9478..>10926
                     /locus_tag="PK399_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW13168.1"
                     /translation="MNQTAPTARMPRQIPFIIGNEACERFSFYGMRNILVQFLVSSVI
                     LAYIPEAQREGAAKDVFHSFVIGVYFFPLLGGWLADRFFGKYNTVLWFSLIYCAGHAC
                     LALFEDNRNGFYTGLFLIALGSGGIKPLVVSFCGDQFTTANKHLAKIVFDAFYWVINF
                     GSFFASLLMPLFLQNYGPAVAFGIPGVLMFIATLIFWLGRRQYVRVPPTRGEDPDSFF
                     NVARTALKAQVAGQGRPGLLVAGFGVVLAVAMLICWALHAWFGVSLPFWPEDFHFVIT
                     ACLALGSLIAFGGIGVSMQLERARGQHPDPAVDAVRTVLRILIVFALTTPFWSLFDQK
                     ASTWVIQGKAMVIPHEAWWWPSRIVQTAGQMQALNPIMVMAIIPFNNLLLYPALRRWG
                     FEPTALRRMGTGIAVAGVAWIIAALLQLRIDGGEAVSLSTQVWPYLILTFAEVLVSAT
                     ALEFAYSQATQSMKGVIMAFWYLTSTFGSLWVL"
BASE COUNT         1727 a         3586 c         3775 g         1838 t
ORIGIN      
        1 cccatccaga agaacaccgc tgccatccgt tcgtcggcca gcacgttgcc gcagtaggcg
       61 cttgcgctgt ggatcatcgc cgcgctgtag accagcaggc gattgaaccg attggggacg
      121 cggacatcct cgacgaagct gtcgggcggc acgaaccggg tgcccatcgc ttccaccaac
      181 gtgttgtgcg ggcgattcac ggtgttgccg cccagctggc cggtgggcag gcgctgccga
      241 tagaaactgg tgccgcaatg ctcgggcgcg tcgggggtga ggtagagcac cccggcgtag
      301 cggcacagcg cgcgcgagtc ggtgtgcgga cgggggccgg cttcgcgttc gccgaccacc
      361 tgcacgcaat tgtggttgag cgtcttgccg tccggggtgt tggcgaccca gagcttcttc
      421 atgccggtgg cctggcggac cttgccctcg accagggcca tctcgtcggc gtcgagtgcg
      481 gggagcgcgc gtcggcccgg ccagctttcg gccgtatgcg ggaagcccag cgcccagtcc
      541 tgcttgccga tgcagcgttc gcgcacgtcg cccgcattcg gcaggacatc atcgaagacc
      601 cagtagtcgc ggccctcggt gggcttgcgg tagggcagca tgcgcagtgg cgggaatggc
      661 cggttcatcc gcgcgactat acggtcgccc ggcccgggtt ggtaaactcc gggcccgaac
      721 cgacaggctg gacccatgcg ccgcaagatc gtcgccggta actggaaact gcacggcacc
      781 cgtgccttcg ccaccgcgct ggtcgcggag atcgcggcca cccccgtccc cggcgtcgaa
      841 ctggtgatcc tgccgccgct gccgtacatc ggcgacctca tcgaggattt cgagggtacg
      901 ccgcttgcat tcggtgccca ggatgtcagc agcaacgagc agggtgccta taccggcgag
      961 gtgtcggcct cgatgctggt cgacgttggc gcgcgctatg gactggtcgg gcattccgag
     1021 cgccgccagt accatgccga gagcagcgaa ctggtggcgc tgaaattcca ggccgcgatc
     1081 catgcggggc tgtgcccgat cctctgcctg ggcgagggcc tggagcagcg cgaagcgggc
     1141 cagaccgaag cggtgatcgc cgcgcaattg cacccggtgc tggagcgggt gggggtggcc
     1201 gccttcgcca gggcggtggt ggcctacgag ccggtctggg cgatcggcac cggccgcacc
     1261 gccagccccg agcaggcgca ggccgtgcac gccttcatcc gtggcgaggt cgccgcgcac
     1321 gatgctacaa tcgccggctc gctgcccatc ctgtatggcg gcagcgtgaa gcccgggaac
     1381 gcggccagcc tgttcggaca ggccgacgtc gacggcggac tgatcggtgg cgcctcgctg
     1441 gtcgccgcgg atttcctggc catcgcaacc gctgcggcgg cctgaccgac tacggaaacg
     1501 attcacgatg tggcttcaac tgatcaatgt ggtgtacgtg ctggtcgcga tctcgatggt
     1561 cgtgctgatc ctgatgcagc gcggcgcggg tgcgcaggcc ggctcgggtt tcggcggcgg
     1621 cgcctcgtcc accgtgttcg gctcgcgcgg ctcgtcgaac ttcctcacca agagcaccaa
     1681 gtggctgggc atcctgttct tcgtgattgc catgggcatg gcgatgtacg ccagcaagag
     1741 cgccaccggc accctggatg cgaagcctga cctcggcgtg atgggccagg cggcaccggc
     1801 ctcggcgaag cccgcgccga aggtgccgac cgcaccggcg cagccagcac cgtcggttcc
     1861 ggctcctgcg atcccggtgg ccgcaccgca gtacgcgcaa ccggaggcgg cccagccgca
     1921 gcccgccacg caggttccgg ctcccgcgcc ggcacccgcg gcacccgccc aaggcggttg
     1981 atcggcatta caatgcgggc cgcggcgcag gccgcaacag atttgcccag gtggcggaat
     2041 tggtagacgc actaccttga ggtggtagcg atttaggtcg taggggttcg agtcccctct
     2101 tgggcaccac gaaggaaaaa cacgaaaccc cgctccggcg gggttttttg tgcccggaat
     2161 tccggtcgcc cgctgctacg atgtgaaata tttacaccca cccaccggag cgacgcatgc
     2221 ccaacctcag cacgatcctc aagggtgaga tcacccgcct ggcccgcaag gaaatcaagg
     2281 cggctgtcga tccggtccgc aaggcgaacg caggccatcg aagggagatc gccgagctca
     2341 agcgccaggt cgcatccctg cagcgggatc tcaagtcatc gtccaagccg gcgaaggtgc
     2401 gggaacccga gggggacgca tcccccggga gccagcgctt ctcggccaag ggcctgaagt
     2461 ccctgcgcgc gaagctggga ctgtctgctg ccgagttcgg ccaactggtg ggcgcaagtg
     2521 ggcaatccat ctacaagtgg gaggcgggca aggctgttcc tcgtgcctcg cagcgggccg
     2581 ccttggtcgc catgcgcggt attggcaagc gcgaggctgc caagcgcctc gcctccatgc
     2641 agtaggcatt ccccgcgcgg cgatcgaagc agcggttccc cgcgcccagt ggcgaggcgc
     2701 cgttccaggc accaccgact ggcatgcgag ctgcgccgtt ccagccggcg atcagccctg
     2761 gcgtcatccg gatgccggtc tccgcaggac cgggggcggg ctagcgggcg gcgggaagcg
     2821 gcttcaggat gagcaggcga tcgccgtcgt cgcgcatgat gtggccggcg gtgcgcaggt
     2881 cgcgcaggac gtggttgacc atctcgcgac tcgcgccgac ccggttggcg atttcctgct
     2941 gggtcagcgg acgcgcgatg gcgcgcacgt ttccttcatc gaccgcggcg tcgtcgagca
     3001 gggcgaacac gcgctcgcgg acctggtggg tggcgaggcg gcgggtgctc tgcgacagcg
     3061 tccgcaacct tgcgatgagc ccgaggatgg tgtgctcagc gaagtctggc tccgagcgca
     3121 ccagcgcgcg cagcgcatcg gcgggaacga ccgcgcatcg cgccctggtg agggccttca
     3181 ccgaggccga acgcgcaccg ccgtcgagca gcatctcgcc gaacagttcg atcggcttca
     3241 gggtggcgta gaccacctcg cgaccttgcc ggccatcgac gtacaccttc aattcgcctt
     3301 ccagcaggat gtacacgcca tcaggcgcat cgtgctcctc gaacaggatc tcgcccttct
     3361 cgaaataccg gatgtcgccg tggccggcca tcctggccaa tacatcatgg gtcatctggt
     3421 tgcgggtatc gctcatctcg tccaatcgtc gcggggcacg ctgccgttat aagcggtgcg
     3481 gtggcgatga tggaacacgg aggccgcgga aggggcattt gtgaaatatg aacacatgcc
     3541 gatgaggccc atcgtttagg ctggtcccga ttccctgcgc ggcctgcgtg gggccgcccg
     3601 acgaagagaa gggagggggt tggcatggac gagccgtcga cggcaacggg caacatcgtc
     3661 gcccgctccc gcggcaaggc ccggcagtcg gcgggtgatg ccgaacgcaa tgcgatgtcc
     3721 gacctgtggg acatcatcgc cgcgcaatcg gagatcgcga acgccggcct ggacctcgat
     3781 cgcgtggtcg atgcgatcac ccgcaaggcg caggtcctga ccggctcgac cggggcggtg
     3841 gtggagatgc tccacggacc cgacctggtg tattggtccg gaagcggcag tgttcgcgag
     3901 cacatcgggg tccggatccc ggcgatcggc agcttgtccg ggctttgcgt cgcgaaggga
     3961 gcgctactgg tctgcaccga cagcgagtca gacccgcggg tcaacatcgc cgcgtgccgc
     4021 agggtgggac tgcgctccgc cctggtgacg ccgttgcatt gcgagggcga actggtcggc
     4081 gtgctgaagg tgctctcgaa ccgggcggat gcctacagcc agcgacacgt ggcaatgctc
     4141 gagaccctgg cgtcattcat cgggtccacg ctcaactcgg cgttggagca cgccaagctg
     4201 cgcgacgccc tgtccagggg ctcccacgcg gagcccgatg cgaagccttc gctggacgaa
     4261 gagcgtgcac gcatctccga cctcatccac ggcggcggcg tgtccccggt gttccagccc
     4321 atcgtcgaac tggcgtcccg ccggatcgtc gggttcgagg cattgagccg gttcgctgcg
     4381 ggcatgcacg cacccgcgga cggctggttc gaggcggcga cgcgcgtggg catgtgcctg
     4441 gaccttgaga ctgcctgcat ctcgtcgatc gtggcggccg tgtcgcgcgg cccgcagcgc
     4501 cacggctaca tttccctgaa cgcctcgccg atgacgctca tggaatacga cttcggcctg
     4561 ctgcccgacc gcagcgcaca cggcggttgg gtgctggaga tcaccgagca ttccgaggtc
     4621 gacgattaca cggcactggc ggggcgcgtg caggcgctgc agcgcgcggg cttccgcatc
     4681 gccatcgacg atgccggcgc cgggtatgcc agcctgcggc acgtgctcag gctctcccct
     4741 gacatcgtga agctggacat ctcgatcacc cgcgacatcg acaagatcgt caagcaccag
     4801 caactggctg cggcgatcat cagcttcagc cgcgacaccg ggatcgagct cgtcgccgaa
     4861 ggcatcgaaa ccgaagccga gcgcgacacg ctggcggcgc tgcgggttcc ctacggacag
     4921 ggctacctgt tcggcaagcc gctcccgttg gcctacggct agccgccacg ccctggatgc
     4981 gcggcggtcc gcaaggtcag ggggcggcac cctcgaattc gtcctcgggc agggccagga
     5041 agctccagaa gggaacctcc tgcgtggccc attcggccga ggcttcttcg agcagggtga
     5101 gcagggtgtc gaaactcgct tcgtcgccat cgcgcaggtg ctgcgcgcca tcgaacagca
     5161 cggcgtagcc aggtgcaggc aaccaggaca ggtcgcgcag gctgtcgccc agagcgtccc
     5221 agttgcgggc gcggccgtcg ggcatggcga gtacctccgc gatgcgcgcc agcaaggcgg
     5281 ccttgccagc gcagtcgccg aggtcgatcc gacgcgccag caacccggca tcgcgcgcgg
     5341 cgacgtctag ggtcgcgagg tcatccccgg tgacgaagaa caccccggcg agcagcgggt
     5401 cgcccaggtc cagctcgacg ccgggatcgc tcatggcatg cctccaggca cctcgaagcg
     5461 gcggaaactg tcgtagtggt cgtcggtgta gtaccactcg cgcggtggct gcccgccggt
     5521 gacgatgcgg cgcgcgccgc gatggtcgag gccgggtgta tcgacggtgt actcgcggta
     5581 ccagccgcgc ggctggcggg gcaggtggcc ttcgcgattg ccgaacacgc tgccgtcctg
     5641 gcggtgcggg aacggaccac cgcgccggat caggtcgacg gtgcgatgcg cttgcgccgg
     5701 caggaaatcg ggaagcgcgg gcgatgcggc ctcgcgcatt ccgctgggcg acggcgtcgc
     5761 caccggcgct ggcccggtgg tcggcaagtg cgctgcggca ggggcttcgc cgttggcggg
     5821 ccacagcttc catgccacta ccagcgcgat ggcgagcagc acccatgcca gcggattgcg
     5881 cctgcgactt gcccgcatgg tcaatgcggg cgtatcacga agggcggatg ctcgttgagc
     5941 gtgccgtcct cctcgaggat ggcgtagccg cggcggtccg ggtcgtacag gaccggcacc
     6001 agcggctgga acagtgggtg gcgcacgaac ttcagttccc agccgtagcg ttccagctca
     6061 cccagtgtgt cgcgctgggc ttcattgagt tcgcgacgca attcccggca ggaatggtgc
     6121 ggcgaatggc ggcgttcggt ctgggccatg ggttccccct gtccgacgat gcgttgcggg
     6181 cgcagaatgc gcccggtcac tcccgggacg caagccatgc ccacggatcc ccgcatcgat
     6241 gcctacatcg aacgcgccgc gccgttcgcg cggccgatcc tggtccacgt gcgtgcgctg
     6301 gtgcaccagg cctgccctgg cgtggaggaa accatcaaat ggggcatgcc gaccttcatg
     6361 catgccggcg gcatcctgtg cgggatggcg gcgttcaagc agcacgcctc gttcgggttc
     6421 tggaagcacg ccatggtggt cggcgagggc gaacgtgcgg atgagaagcc gcgcgatggc
     6481 atgggcagct acggcaagct gacctcgctg gacgacttgc cggcgaagaa ggccttgctg
     6541 gcgcacatcc acaaggcgat gaagctcaac gaggacgggg tgaaggtacc ggctgcgcgc
     6601 aaggccgcgc caaggccggc accggaaacg ccggccgacc tgctcgccgc gtttaagcag
     6661 gacaaggcgg cgcaggcgac cttcgatgcg ttcccgccga gctgcaagcg cgagtacatc
     6721 gaatggatcg tcgaggccaa gcgcgaggac acccgcgcga agcggctggc acaggccgtg
     6781 gaatggctgg ccgaaggcaa gcgtcgcaac tggaagtacg agaactgttg aacccggttg
     6841 gtgcatcgcg tcggcgcacg caatgcatgc agtgatcagt acacggtgtc cgtgcgcagc
     6901 accggatagc gattggtgcc ggcatcccag gcgggatggc ggcggtagaa gaagtccagt
     6961 cgcgcgccgg ggctcctggc gaactcggca tcctcgcgca gcttgcgttc gaattcggcc
     7021 ttcagcgcgg gatcctgcgc cagcatcagc cgcgcctgtt cctcggccac gtagccttcc
     7081 atgtattcct tgcgctcgaa cgcattgttg aagtcgcccc aggcggccat cgcatcgccg
     7141 gcggtcggtt ccaggatgtt caccagcaag cgcgacttcg cctgcgcgat cggcacgtaa
     7201 aggccgcccg cggcgatttc cgccgaatgc ttcgtccacg cgcctttcag ggtgacgcgc
     7261 tggtggcctt cgaccgaggt cggcgagaac tgcacggcgt cggcgacgaa ggcctgtgca
     7321 ttcggatcgc gcaacggcgc ggtcagcacg cggaacgtga tgccatgcgt gagcaattgc
     7381 cgggccaccg cgactgcgta ggactgcggc accaggtagc cgcccttcgg tgcgacgacg
     7441 ctcgtcgccg gcacgatctc gtcgcgcaaa gggatcttcc acacctgcgg cgtggtttcg
     7501 tcgtagcgcg tcatcgtgcc gccggaaatg tccgatggcg tgcgcgtgta cgcgtagccc
     7561 aggaaatcga tggtgcgcac cttgtcggtg gccttgtact cgaccggcac tgattgcccg
     7621 ccgagtgccg ccgcgcgcgc atccgcatcg cgcgccagct gcagccagcg ggtgccattc
     7681 gtggccgtca attccagcgc atccagcacg gtgttgtagg tgctgcgcac gcgctccgga
     7741 taggtgcgcc aggaatgcgt ctcgaccagg atggccagcc ggttgcgcag cgggaaatag
     7801 ccgttggaga agcgcggggt gctgacgcca tggatgaagc ctgaggccgg atcatcgtcc
     7861 tccacgaagc tcgggtagaa gccgaccggc agcgagccct gggtggcgag tttcgcgatg
     7921 atgccgtcct tcagcgcgtt gccggcttcg cgcagggcga tgtcgccgga gttggtgggt
     7981 tcaccggtga tcgagatgtc gtgctggaac ttggcgccat cggtgacgtg caggtcgagc
     8041 atcgccagcg gatcccactg gttcaccaag cccagcatcg cctgcatttc cgggctgtcg
     8101 gccttcacgt aatcgcggtt gaggttgtag cgctgtgccg tgacgcgata gcccatctgc
     8161 tccgggccgc gctggttggg ccggttccac gcacggaagt tctcgtggcc gtcggcgttg
     8221 aacaccggca cgaacagcag caccgccttg tccagcaggc ctgctccgac cttgccctgc
     8281 agcaggtcgc ggatgagcca gaacccggcg tccttgccat cgatctcgcc ggcatggatg
     8341 ccgccctgca ccagcaccac cggcaggtcg cgcgcctgcg ccgccttggg atccagcgcg
     8401 ccgctggtgg acaccgccat cgccttcatc ggccggccct ccggggtggt gccgaaggtg
     8461 aaacagcgca ccgactgcgg gtaggcctgc tcgaatgcat cgcacagcgc gatgacctcg
     8521 tcgtagcgac cggtctgctt gaagccgctg cgctcggcgg tggtggtcag cgcgacgctg
     8581 cgcgcgaagg cgggcgaggt gcccatcagc agcgggcagg cgagcagggc ggtggcaagc
     8641 agggcacgac gcatggtggc tctccggcgg gattggccga tggtaaccgc ggtgccggtg
     8701 ggcacgggcc tgcccatggt catgccgcat cccgccggcc acgcatgcga acatgcgcca
     8761 tcgccacgga gcccattcgc atgagcgcgt cgaacacccg catgaccctg tactacgccc
     8821 ccggcgccgc cagcttcctg gtgcactggc tgctgatcga cctcgacgcg ccgcatgcgc
     8881 tgcacctggt cgataccgcc gccagggcgc agaagtcgcc cgagtacctg gcgctgaatc
     8941 cgaatggggt ggtgccgacg ctggtcgtcg atggcaggcc gcactacgag gccgcggcgc
     9001 tggcgatgct gctggccgaa cgtcatccgg atgccgggct ggcaccggcg ttcgacgatc
     9061 cgcgccgtgc ggactacctg cagtggatgt tcaacctggc caacatggtg caaccgctgt
     9121 tccgccagtg gtggtatccg ggcgagccgg ccggcgaagc gaatgcggac gcgatccgcg
     9181 cgcattgcgc gccccgcatc gaggccgaat ggcagcgcat cgacgaccac ttggcggcga
     9241 acggcccgca cctgctgggc gagggcctga gcgttgccga tttctacctg gtgatgttga
     9301 tgcgctggtc gcgcaacatg ccgaagccgg cgaccgaatg gccgcacctg gccgcgctgg
     9361 cgcagcgcct gaaggcacgc ccttccttca aggcgctcta cgagcgtgaa ggcctggccg
     9421 aatgggcctg agcgttcgat cggttaggct tgccgggttc gcacgacaag gccttgcatg
     9481 aaccagaccg ccccgaccgc gcgcatgccg cggcagattc ccttcatcat cggcaacgaa
     9541 gcctgcgagc gcttcagctt ctacgggatg cgcaacatcc tggtgcagtt cctggtcagc
     9601 agcgtgatcc tggcctacat ccccgaggcc cagcgcgaag gcgcggccaa ggatgtgttc
     9661 cacagcttcg tgattggcgt gtatttcttc ccgctgctcg gcggttggct ggccgaccgg
     9721 ttcttcggaa aatacaacac ggtgctctgg ttctcgctga tctattgcgc gggccatgcc
     9781 tgcctggcgc tgttcgagga caacaggaac gggttctaca ccggcctgtt cctcatcgcg
     9841 ctgggcagcg gcggcatcaa gccattggtt gtcagtttct gcggcgacca gttcaccacc
     9901 gccaacaagc acctcgccaa gatcgtgttc gacgccttct actgggtgat caacttcggc
     9961 agtttcttcg cctcgttgct gatgcccttg ttcctgcaga actacggacc ggcggtggcc
    10021 ttcggcatcc cgggcgtgct gatgttcatc gccaccctga ttttctggct gggccgtcgg
    10081 caatatgtcc gggtcccgcc cacccgcggt gaggatccgg attcgttctt caacgtggcc
    10141 cgcaccgcgc tgaaggcgca ggtggcggga caggggcggc ccggcctgct ggtcgccggc
    10201 ttcggcgtgg tgctggcggt ggcgatgctg atctgctggg cgctgcatgc atggttcggg
    10261 gtcagcctgc cgttctggcc ggaggatttc catttcgtga tcaccgcctg cctggcgctg
    10321 ggttcgctca tcgcgttcgg cggcatcggc gtgtcgatgc aactggagcg tgctcgcggc
    10381 cagcatccgg atcccgcagt ggacgctgtc cgtacggtgc tgcggatcct gatcgtgttc
    10441 gcgctgacca cgccgttctg gtcgctgttc gaccagaaag ccagcacctg ggtgatccag
    10501 ggcaaggcga tggtgatccc gcatgaggcc tggtggtggc cgagccggat cgtccagacc
    10561 gccggacaga tgcaggcgct caacccgatc atggtcatgg ccatcattcc gttcaacaac
    10621 ctgctgttgt atccggcgct gcgccgctgg ggattcgagc cgacggcgct gcgccgcatg
    10681 ggcacaggca tcgcagtggc cggtgttgcc tggatcatcg cggcgctgct gcaattgcgg
    10741 atcgacggtg gcgaagcggt gtcgctttcg acccaggtgt ggccttacct gatcctcacc
    10801 ttcgccgagg tgctggtttc cgccactgcg ctggagttcg catacagcca ggccacgcag
    10861 tcgatgaagg gcgtgatcat ggcgttctgg tacctcacca gcaccttcgg cagcctctgg
    10921 gtgctg
//
LOCUS       DAOOCX010000079        10787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5717_length_10787_cov_8.201919, whole genome shotgun sequence.
ACCESSION   DAOOCX010000079 DAOOCX010000000
VERSION     DAOOCX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5717_length_10787_cov_8.201919"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..211
                     /gene="ffs"
                     /locus_tag="PK399_08355"
     ncRNA           115..211
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK399_08355"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            417..2153
                     /gene="dnaX"
                     /locus_tag="PK399_08360"
     CDS             417..2153
                     /gene="dnaX"
                     /locus_tag="PK399_08360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPW13169.1"
                     /translation="MSYLVLARKWRPKRFAELVGQEHVVRALTNALQTGRVHHAFLFT
                     GTRGVGKTTIARIFAKSLNCEQGTSADPCGECETCLAIDAGRYIDLLEIDAASNTGVD
                     NVRELIENAQYMPSRGKYKVYLIDEVHMLSKPAFNALLKTLEEPPGHVKFLFATTDPE
                     KLLVTVLSRCLQFNLKRLDEAQIRGQIVKILGAEGIEADDAAVRQLAHAADGSLRDAL
                     SLLDQAIGYTGGKLEGAAVATMLGTVDRSRVQALLAALADGDGARLLDEATQLAEFSP
                     DWGSVLDAVSEALHRIQVKQLVPAVEVASDAIDIEALATQLRPELVQLWYQMALNGRR
                     DLGYAPSPRSGFEMSLLRMLAFRPETAGVGAQPSVPKPAVAPPSSPQERSAAAERARA
                     VMAESLPKAAKPSPAPVPEPEPSRESTPPVRVAEPPPPRSVESVVAATPLPANTLLVT
                     DSEHWLDLVARSSLRGPAKMLAEHAAFVAHGDGVLRLSLATEHEHLKSPSLVLQLGDA
                     VSRLLGGNVQLRFESGPARGETASVRNSRERELRQAGAEQGFAADPAIQRLVQVHGAQ
                     LLPESIRPLGDT"
     gene            2191..2511
                     /locus_tag="PK399_08365"
     CDS             2191..2511
                     /locus_tag="PK399_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPW13170.1"
                     /translation="MRGDIAQLMQQAQKMQENMQRAQDEIAALEATGNAGGGMVSVTL
                     GGRMDCRKVRIDPSLFSDPEMAEDLVAAAFNDAVNRINAESQAKMSAATAGMPIPPGM
                     KLPF"
     gene            2580..3218
                     /gene="recR"
                     /locus_tag="PK399_08370"
     CDS             2580..3218
                     /gene="recR"
                     /locus_tag="PK399_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPW13171.1"
                     /translation="MTTSLLEQLIDALRVLPGVGQKSAQRMAYHLLERQRDGGRRLAD
                     ALAEAMEHIGHCERCRDFSETSVCPTCASNARDVHLLCAVETPADRLAIEQATGFRGL
                     YFVLQGRLSPLDGIGPRELGLDRLAQRLEEGEVQELIVATNPTVEGEATAHYLAQLAR
                     ARGVHPSRLAHGVPLGGELEYVDRGTLSHAFGSRSEIPDERSNGERAHGEHN"
     gene            3229..3582
                     /locus_tag="PK399_08375"
     CDS             3229..3582
                     /locus_tag="PK399_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPW13172.1"
                     /translation="MSDTIFHKIRHGEIPADIVYEDEHLFAFRDIAPQAPVHVLFVPK
                     TDFATLNDVPDDQAIVVGRLATAAARYARAQGFAEDGYRIVMNCNGDGGQTVFQIHLH
                     LLAGAPLGRFGTQAT"
     gene            complement(3589..4014)
                     /locus_tag="PK399_08380"
     CDS             complement(3589..4014)
                     /locus_tag="PK399_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPW13173.1"
                     /translation="MGLELVSTGAIRDLDRLLARMHPSLHPGRYVFTTLPRGQAIDPT
                     SIVASIREAEGLSVVVTETDALALGLPMAFTAAWITLEVHSDLAAVGLTAAFSQALAK
                     AGIGCNVIAGTIHDHLFVPFDQANDAMAVLHGLQGHAAA"
     gene            complement(3996..4484)
                     /locus_tag="PK399_08385"
     CDS             complement(3996..4484)
                     /locus_tag="PK399_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HPW13174.1"
                     /translation="MTFRPIATGIAAILLAACAATQPRPLQGQFDALTPHQAVVGDHT
                     GASVRWGGRILRTEPRTDRTCFEVISTKLNADGRPVWASDDTWGRFIACRTGFYDPAL
                     FEPNREVTFTGRIEGYEKRKVEGYVYDFPRVAADVVYLWPKRERVDVVTRPSPWPWAW
                     NW"
     gene            complement(4560..6488)
                     /locus_tag="PK399_08390"
     CDS             complement(4560..6488)
                     /locus_tag="PK399_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HPW13175.1"
                     /translation="MQATARRHVLLATATCLMPLLLQLPPELGWGFGVAAVLVAAASW
                     RRPLQAVFRILLGIGVLLAVVAVSPGIGRDTACALLAGMLALKPSETMTLRDGRSLVG
                     FALFAPFATFLLDQGPLSLALALAAVLLALLALQCLARDEGEVRVADGPRWWSGSTGV
                     LRLLALGLPLALAAFWLFPRLPSPLWGLPERALAKPGLSDNMTPGGFLDLMTDDSPAA
                     RVQFFGAEPRPQDMYWRGPVLWNFDGRAWTQAHWLQPAPAAPMRPAGPGWDYQLDLEP
                     TESRQMIVLDLPTAVPANAHVSIDFSTWSDTPLNNVSRWRMRSAPPAAAEPDLHPMLR
                     DRALRLPRGFNPRTLALGRQWRREAGRDATGVADARIADRALTWIRRDFAYTLKVPLA
                     GRNEVDDFLFDRKQGYCEHFSSAFVVLMRASGVPARVVTGFAGGYRNPIGGYWLVLNS
                     DAHAWAEVWLPRRGWVRVDPTAAVAPERVYDTLADRQPGRIGAFDGLVPMFNAGDWLR
                     RGWNDFVLGFNAQRQQDLLKPFGLGKLKGQHLILLFALLAGLALAWMAWLIARGERER
                     DPLLRAWHRLDARYRRLGRGRAQHEPAQAWAERVAADQPRAGEHLAILSRRFSDARYA
                     PQPAELQGLLRDLGRHRP"
     gene            complement(6508..7464)
                     /locus_tag="PK399_08395"
     CDS             complement(6508..7464)
                     /locus_tag="PK399_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPW13176.1"
                     /translation="MRDALRRRLQAWMRPRQPEPLPVLLRRSRIYVLPTATGLFFGLL
                     LGTMGLGALNFNNNPALLLALLLAGAAQASLIAAHMQLSGLRLLAVSADPVAAGEDLR
                     LKIALDTVDARIRRGLRVAAAGDALHTTLDSHGTTVELHLPTQRRGWLALPRLELGTV
                     QPLGLARAWSYVWPNQSVLVYPAPEAQAPPLPAPAGGKGRPRPARAGDDVHHLRNYRA
                     GDTPRSVAWKASARRDVLLVREYEQQLSGELVLDWQATHGLPHEQRIRRLARWVDDAE
                     REGRRYGLRLPGQPVIAMGLGALQRHRCLRALALLPGEPTHG"
     gene            complement(7466..8416)
                     /locus_tag="PK399_08400"
     CDS             complement(7466..8416)
                     /locus_tag="PK399_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW13177.1"
                     /translation="MSTSPAPDAILPEALGAALRDAQAQVNRLVLGKPQQVRHAFVAL
                     LADGHLLIEDLPGLGKTTLAHAMAATLGLGFARVQFTSDLLPADILGVSVYEAQARRF
                     EFHPGPVFNHVLLADEINRAPPRTQSALLEAMAEHQVSIDGRTHALPDPFLVIATQNP
                     VDLAGTYPLPDSQLDRFLLRLALGYPDEATERALLAGSDRRELIAQAQPLLDAERILD
                     LRRAVQAVHASDPLVAYVQALVGRSRRHPGVRVGLSPRAGLALLRAARAHALLLGRGH
                     ALPEDVQALFGAVAEHRLVGEADASTTTLAREILHAVAID"
     gene            8461..10302
                     /locus_tag="PK399_08405"
     CDS             8461..10302
                     /locus_tag="PK399_08405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPW13178.1"
                     /translation="MREANDATTARRGFLALWLVVFAIKLALAARLPLFVDEAFYWQE
                     GRHLAWAYSDLPGLSAWLAAVGDAIAPGNVLALRLPFLLVGAAVPWLVARMTMRELDA
                     RSGWWAGSLALLLPLAGTLGLLALPDAPMLLASVLCLDAGLRLLRRVDAFAAGELALG
                     LVVGGLTHYRFVAVIAVGLFALLAVRQGRAVLRSRWTWAAVAIGALAWLPLLLWNLHN
                     ADAGLRFQLVDRHPWQFSGEGFRLGRMQLLLATPLLLAAMVVAAWRGMRDARPGVRYL
                     SLSGAMIVLGFLALGFFADRERASFHWPLPGYIALLPLVPAVLGRWHRGWRIAAWTIA
                     ALGLATVLAFYVAASVPALREQAASRNFHPTNFAGWDVLAREVDARLATMPEGTRVLA
                     GNFKIGAELGFARGDADIAVLDHPLNHKHGRAPQLALWGLAHDTRDSLGDTPLLLVAS
                     SNDVDFRDLLAHYHRLCEQVGPLPPPRVLNIDHGRQRFLLFELGTSRRDGPCTTPAIA
                     FLDAPAPSARVGGAFQVAGWAFKDGVGLRGVDVLVDGHVVAHARYGEANTGVADFWRG
                     STDPNHPRVYFRAQVAGLPAGRHWLGLRLHGADGSSEDWPSQLVVVR"
     gene            complement(10299..>10787)
                     /locus_tag="PK399_08410"
     CDS             complement(10299..>10787)
                     /locus_tag="PK399_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HPW13179.1"
                     /translation="VDETPLPAESPRALANRLAVAKARDVAHGLPDDAWALGSDQVAD
                     LDGVPLGKPGGRDAAIGQLRTMSGRTVRFHTALCLAHADGRVFTDIDLTEVQFRLLDD
                     AAIERYVDAEQPYDCAGSFKSEGLGITLFEAIDNRDPSALVGLPLIATCRLLRQAGFV
                     LP"
BASE COUNT         1634 a         3644 c         3914 g         1595 t
ORIGIN      
        1 tgagttacat gatcggattg tgaggggttg ggggcgcttg gtacgctaag tcgcggaccc
       61 tctctccgcc atttccaagt tccaccgtcg caccagttcg tctccatggt ggccccgtcg
      121 gcccctcgcg acgctaggtc gaaaactccg ccagggccgg aaggcagcaa cggtatcgac
      181 tgatgcgggc gccgaggtca gccggcgggg ccatcgccat tcatgggctc cgcccatgaa
      241 tcccaaagtt tgcttcgcaa acttcgggcc ccggctccta cttccagaac ccccggcgct
      301 gcgcgccacc cccttgactc aagggggttg aacatcaaga gcgagcacat cgcggcaccc
      361 gactcccggc tccggattca tcgaagcggg acgcgcgtgc atgcgacaat gcaggcatgt
      421 cctacctcgt cctcgcccgc aagtggcggc ccaagcgttt cgccgaactg gtcgggcagg
      481 agcacgtcgt gcgtgcgttg accaacgcgc tgcagaccgg ccgggtccac catgccttcc
      541 tgttcaccgg cacgcgcggg gtcggcaaga ccacgatcgc gcggatcttc gccaagtcgc
      601 tgaattgcga gcagggcacc agtgcggatc cgtgcgggga atgcgagacc tgcctggcga
      661 tcgacgccgg ccgctacatc gacctgctgg agatcgacgc ggcatccaac accggcgtgg
      721 acaacgtccg cgagctgatc gagaacgcgc agtacatgcc gtcgcgcggc aagtacaagg
      781 tctacctgat cgacgaagtg cacatgctgt cgaagccggc gttcaacgcg ctgctgaaga
      841 cgctggaaga gccgccgggc cacgtcaagt tcctgttcgc cacgaccgat ccggagaagc
      901 tgctggtgac ggtgctgagc cgctgcctgc agttcaacct gaagcggctg gacgaggcgc
      961 agatccgcgg gcagatcgtc aagatcctgg gtgcggaagg catcgaggcc gatgacgcgg
     1021 ccgtgcggca gctggcgcat gccgccgacg gcagcctgcg cgatgcgttg tcgctgctcg
     1081 accaggccat tggctacacc ggcgggaagc tcgagggcgc cgcggtggcg acgatgctgg
     1141 ggacggtcga tcgcagccgc gtgcaggcgc tgcttgccgc gctggccgac ggcgatggtg
     1201 cgcgcttgct cgatgaggcc acgcaactcg cggagttctc gccggattgg ggcagcgtgc
     1261 tggacgcggt gtccgaggcc ttgcaccgga tccaggtcaa gcagttggtg cctgccgtcg
     1321 aagtcgccag cgatgcgatc gatatcgaag cgctggcgac gcaactgcga ccggaactgg
     1381 tgcagctctg gtaccagatg gcgttgaatg gtcgtcgcga cctgggttac gcgcccagcc
     1441 cgcgcagcgg cttcgagatg agcctcttgc ggatgctggc gttccggccg gaaaccgccg
     1501 gcgtgggggc gcagccatcg gtgccgaaac cggcggtcgc gccgccgtcg tccccacagg
     1561 agcgcagcgc cgccgcggaa cgcgcgcgcg cagtgatggc cgagagcctg ccgaaggccg
     1621 caaaaccgtc gcctgcaccc gtgcccgagc ccgagccgag tcgcgagtcc acgccgcccg
     1681 tgcgggtggc ggaaccgccg ccgccacgga gtgtggagtc ggtcgtggcc gccacgccgc
     1741 ttcccgcgaa taccttgctg gtcaccgaca gcgaacactg gctggacctg gtggcgcgca
     1801 gcagcctgcg cgggccggcg aagatgctgg ccgagcacgc ggccttcgtg gcccatggcg
     1861 atggggtctt gcggctctcg ctggccaccg agcacgaaca cctgaaatcg cccagcctgg
     1921 tgttgcaact gggcgatgcg gtctcccgcc tgctgggcgg caacgtgcaa ctgcgcttcg
     1981 agtccggccc ggcgcgtggc gagaccgcca gcgtgcgcaa cagccgcgaa cgcgagctgc
     2041 gccaggcggg cgcggagcag ggcttcgccg ccgatcccgc gatccagcga ctggtgcagg
     2101 tgcacggcgc gcagctgctg ccggagtcga tccggccgct tggtgacacc tgatccctcc
     2161 attccgatac acgacgacag gaacgacgac atgcgtggag acatcgccca gctgatgcag
     2221 caggcgcaga agatgcagga aaacatgcag cgcgcgcagg acgagatcgc cgcgctcgag
     2281 gccaccggca atgccggcgg cggcatggtc agcgtgaccc tgggcgggcg catggactgc
     2341 cgcaaggtgc gcatcgaccc gtcgctgttc tccgatccgg agatggcgga ggacctggtg
     2401 gcggccgcgt tcaacgacgc ggtcaaccgg atcaatgccg aatcgcaggc gaagatgtcg
     2461 gccgccaccg cgggcatgcc gatcccaccg ggcatgaagc tgccgttctg atgtgcttgc
     2521 cgtcgtcctg atttttttca agtcattccc gccgtcgcgg gaatgacgaa cctttgccca
     2581 tgacgacctc cctcctcgaa caactcatcg acgcattgcg cgtgctgccc ggcgtcggcc
     2641 agaagtcggc gcaacgcatg gcctaccacc tgctggagcg ccagcgcgac ggcgggcgac
     2701 gcttggccga tgcgctggcc gaggcgatgg aacacatcgg gcattgcgag cgttgccgcg
     2761 atttcagcga aacctcggtg tgcccgacct gcgccagcaa cgcgcgcgac gtgcacctgc
     2821 tgtgcgcggt ggagacgccg gccgaccggt tggccatcga gcaggccacc ggcttccgcg
     2881 ggctgtactt cgtgttgcag ggacgattga gtccgctcga cggcatcggc ccgcgcgaac
     2941 tcgggctgga ccggctggcg cagcggctgg aggaagggga ggtgcaggag ctcatcgtcg
     3001 ccaccaaccc caccgtggaa ggcgaggcga ccgcgcacta cctggcgcag ctggcgcggg
     3061 ctcgcggcgt gcacccgagc cggcttgccc acggcgtccc gctgggcggc gagctggaat
     3121 acgtggatcg cgggacgttg tcgcatgcgt tcggcagccg cagcgagatt cccgacgaac
     3181 gcagcaacgg cgagcgcgcc catggcgaac acaactgagg aacgtccgat gagcgacacc
     3241 atcttccaca agatccgcca tggcgagatc ccggccgaca tcgtgtacga ggacgaacac
     3301 ctgttcgcct ttcgcgacat cgccccgcag gcgccggtgc acgtgttgtt cgttcccaag
     3361 accgacttcg ccacgctcaa cgacgtgccg gacgaccagg ccatcgtggt gggtcggctt
     3421 gcgaccgcag cagcgcgcta tgcacgcgcg cagggcttcg ccgaggacgg ctatcgcatc
     3481 gtcatgaact gcaatggcga cggcgggcag accgtgttcc agatccacct gcacctgctc
     3541 gccggcgcgc cgctggggcg cttcggcacg caggccacct gagggcgatc aggcggcagc
     3601 gtgcccctgc agcccgtgca acacggccat cgcgtcgttc gcctgatcga aggggacgaa
     3661 gaggtgatcg tggatcgtgc cggcgatcac gttgcagccg atcccggctt tcgccagcgc
     3721 ctgcgaaaac gcagcagtca agccgacggc cgcaaggtcg gaatgcacct ccagggtgat
     3781 ccacgcagcg gtgaacgcca tcggcaggcc gagcgccagc gcatccgttt cggtcacgac
     3841 gacggacagg ccttccgctt cgcggatgga cgccacgatg gatgtcggat cgatcgcctg
     3901 gccgcgcggc aaggtggtga acacatagcg tcccgggtgc agcgacggat gcatcctcgc
     3961 cagcaaccgg tccaggtcgc ggatcgcgcc tgtgctcacc agttccaggc ccacggccaa
     4021 ggcgaggggc gggtgaccac gtccacgcgt tcgcgcttgg gccacaggta gaccacgtcc
     4081 gcggcgacgc gcgggaagtc gtagacgtaa ccctcgacct tgcgcttttc gtagccttcg
     4141 atccggccag tgaaggtcac ttcgcggttg ggttcgaaca gcgccgggtc gtagaagccg
     4201 gtgcggcagg cgatgaagcg gccccaggtg tcgtcgctgg cccagaccgg gcggccatcg
     4261 gcattgagct tggtggaaat cacttcgaag caggtgcggt ccgtgcgtgg ttccgtgcgg
     4321 aggatccggc cgccccagcg cacgctggcg cccgtgtggt cgccgacgac ggcctggtgc
     4381 ggcgtcagcg catcgaactg gccctgcagc ggcctcggtt gggtggcggc acaggcggcc
     4441 agcaggatgg cggcgatgcc ggtggcgatc gggcgaaagg tcatggcggt tccggggagg
     4501 caggtgcgat ggacgacggc gtcaggccgg caccgtaaca ggcatcgatc aaggccgcgt
     4561 cacgggcgat ggcgtccgag atcccgcagc aacccctgca gttcggccgg ctgcggtgca
     4621 tagcgcgcat ccgagaagcg ccggctcaag atggcgagat gctcgccggc acgcggttgg
     4681 tcggcggcga cgcgctcggc ccacgcctgc gccggttcgt gctgcgcgcg gccacgcccg
     4741 agccggcggt agcgtgcgtc gagccggtgc caggcccgca gcagcgggtc gcgttcgcgt
     4801 tcgccgcggg cgatcagcca cgccatccag gccagggcca ggccagccag cagcgcgaac
     4861 agcaggatca ggtgctgccc cttcagcttg cccaacccga agggtttcag caggtcctgc
     4921 tggcgctgcg cgttgaagcc cagcacgaag tcgttccagc cacggcgcag ccagtcgccg
     4981 gcattgaaca tcggcaccag cccgtcaaac gcaccgatcc gacccggctg gcggtcggcc
     5041 agggtgtcgt acacgcgctc cggcgcgacc gcggccgtcg gatccacccg cacccagccg
     5101 cgccgcggca gccagacctc ggcccaggca tgcgcgtcgg aattcagtac cagccagtag
     5161 ccaccgatcg ggttgcgata gccgccggcg aacccggtca ccacgcgcgc cggcacgccc
     5221 gacgcgcgca tcagcaccac gaaggcggaa ctgaaatgct cgcagtagcc ttgcttgcga
     5281 tcgaacagga aatcgtcgac ttcgttgcgg ccggccagtg gcaccttgag ggtgtacgcg
     5341 aagtcgcggc ggatccaggt cagcgcgcgg tcggcgatgc gtgcgtcggc tacgccggtg
     5401 gcatcgcggc cggcttcgcg ccgccactgc ctgcccagcg ccagggtgcg cgggttgaag
     5461 ccccgtggca gccgcagcgc gcggtcgcgc agcatcgggt gcaggtcggg ttccgctgcg
     5521 gcgggggggg ccgagcgcat ccgccagcgt gagacgttgt tgagcggggt gtcgctccag
     5581 gtactgaaat cgatgctgac atgcgcattc gccggcaccg ccgtgggcag gtcgagcacg
     5641 atcatctgcc ggctctcggt gggctccagg tcgagctggt agtcccagcc tggccccgcc
     5701 gggcgcatcg gcgcggcggg tgccggctgc aaccagtgcg cctgggtcca ggcacgcccg
     5761 tcgaagttcc acagcactgg tccgcgccag tacatgtcct gcggccgagg ttccgcgccg
     5821 aagaactgca cccgtgccgc cgggctgtcg tcggtcatga ggtccaggaa cccgccgggc
     5881 gtcatgttgt cggacaggcc gggcttggcc agcgcacgct cgggcaggcc ccacaacggc
     5941 gaaggcaggc gcgggaacag ccagaacgcg gccagtgcca gtggcaggcc cagcgcgagc
     6001 aggcgcagga cgccggtcga tcccgaccac cagcgcgggc catcggcaac gcggacctcg
     6061 ccttcgtcgc gggccaggca ttgcagggcg agcagggcca gcagcacggc ggccagcgcc
     6121 agtgccaggc tgagcggtcc ctggtcgagc aggaatgtgg cgaatggcgc gaacagggcg
     6181 aagccgacca ggctgcggcc atcgcgcagg gtcatggttt ccgatggctt cagcgccagc
     6241 atgccggcga gcagggcgca ggcggtatcg cggccgatcc cgggagacac cgcgaccacg
     6301 gccagcagca cgccgatgcc cagcaggatg cggaagacgg cctgcagcgg tcgccgccag
     6361 gaggccgccg cgaccagcac ggcagcgacg ccgaagcccc agccaagttc gggcggcagt
     6421 tgcagcaaca gcggcatcag gcaggtcgcg gtggccagca gtacatggcg ccgcgccgtg
     6481 gcctgcatcg gcggcgaggg cgtgcgctca gccatgcgtg ggctcgcccg gcagcagggc
     6541 gagtgcgcgc aggcagcgat gccgctgcag cgcgcccagg cccatcgcga tcaccggctg
     6601 gcccggcagg cgcaggccgt agcgacgacc ctcgcgctcg gcatcgtcca cccagcgggc
     6661 gagccggcgg atgcgttgtt cgtgcggcag gccgtgggtc gcctgccagt cgagcaccag
     6721 ttcgccgctg agttgctgct cgtattcgcg gaccagcagg acgtcgcggc gggccgacgc
     6781 cttccacgcc accgagcgcg gggtgtcacc ggcgcggtaa ttgcgcaggt ggtggacatc
     6841 gtcgccggcg cgcgcgggcc gtggccggcc cttgccgccg gcgggggcgg gcagcggcgg
     6901 tgcctgtgcc tccggcgccg gatacaccag caccgactgg ttcggccaga cgtacgacca
     6961 cgcacgggcc aggcccagcg gctgcacggt gcccagctcg agccggggca aggccagcca
     7021 gccgcgacgc tgcgtgggca ggtgcaactc gacggtggtg ccgtggctgt cgagggtggt
     7081 gtgcagggcg tcgcctgccg cggccacgcg cagtccacgc cggatgcgcg catcgacggt
     7141 gtccagggcg atcttcaggc ggaggtcttc gccggcggcg accgggtcgg cggagaccgc
     7201 cagcaggcgc aggccggaca actgcatgtg cgcggcgatc aggctggcct gcgcggcacc
     7261 ggccagcagc agggccagca acagcgccgg gttgttgttg aagttcagcg cccccaggcc
     7321 catcgtcccc agcaacaggc cgaagaacag gccggtcgcg gtgggcagga cgtagatgcg
     7381 gctgcgccgc aacaacaccg gcagcggctc cggctggcgg ggccgcatcc atgcctgcag
     7441 gcgacggcgc aacgcgtcgc gcatctcagt cgatggccac cgcgtgcagg atctcgcgcg
     7501 ccagcgtggt ggtcgaggca tcggcctcgc cgaccaggcg gtgctcggcg accgccccga
     7561 acagggcctg cacgtcttcg ggcagcgcgt gcccgcggcc gagcagcagc gcgtgcgcgc
     7621 gcgcggcgcg cagcaatgcc aggccggcac gtggcgacag gcccacgcgg acgcccgggt
     7681 gtcgccggct gcggccgacc agcgcctgca cgtaggccac caggggatcg ctggcatgca
     7741 ccgcctgtac cgcgcggcgc aggtccagga tgcgttcggc atccagcaac ggttgcgcct
     7801 gcgcgatcaa ttcgcggcgg tcgctgcctg ccaacagggc gcgctcggtt gcctcgtccg
     7861 gatagccaag cgccagccgc agcaggaagc ggtcgagctg cgaatccggc agcgggtagg
     7921 tgccggccag gtccaccggg ttctgggtgg cgatcaccag gaacggatcg ggcagggcat
     7981 gcgtgcgtcc gtcgatgctg acctggtgct cggccatcgc ttccagcaag gcactttgcg
     8041 tgcgcggcgg cgcgcggttg atctcgtcgg ccagcagcac gtggttgaac accgggccag
     8101 gatggaattc gaagcggcgc gcctgcgctt cgtacaccga gacgccgagg atgtcggcgg
     8161 gtagcaggtc ggaggtgaac tgcacgcggg cgaacccgag cccgagcgtc gcggccatcg
     8221 catgcgccaa ggtggtcttg cccagtccgg gcaggtcttc gatcagcagg tggccatcgg
     8281 ccagcagggc gacgaaggca tgccgcacct gttgcggctt gcccagcacc aggcggttga
     8341 cctgcgcctg cgcatcgcgc aaggccgcgc ccagggcttc gggtaggatc gcgtcgggtg
     8401 ccggggatgt ggacattgga gacctcggta ggttgccgcg agtgtagcga ggtcgggtgg
     8461 atgcgggagg cgaacgacgc gacgaccgca agacgcgggt tcctcgcgct gtggctggtg
     8521 gtgttcgcga tcaagctggc gttggctgcg cggctgccgc tgttcgtgga cgaagcgttc
     8581 tactggcagg aaggccgcca cctggcgtgg gcgtactcgg acctgccggg cctgagtgcg
     8641 tggctggccg ccgtgggtga tgcgattgca cccggcaatg tgctggcgct gcgcttgccc
     8701 ttcctgctgg tcggtgccgc ggtgccgtgg ctggtggcgc ggatgacgat gcgcgaactc
     8761 gacgcgcgca gcggctggtg ggcgggatcg ctggccctgc tgctgccgct ggcgggcacg
     8821 ctggggctgc tggccctgcc cgacgcaccg atgttgctgg ccagcgtgct gtgcctggat
     8881 gccgggctgc ggctgctgcg tcgcgtggat gccttcgccg ccggcgaact ggcgctgggt
     8941 ctggtggtcg gcggactcac ccattaccgc ttcgtcgcgg tgatcgcagt cggcttgttc
     9001 gccttgttgg ccgtgcgcca aggccgcgcc gtgctgcgca gtcgctggac gtgggcggcg
     9061 gtcgcgatcg gagcgctggc atggctgccg ctgctgctgt ggaacctgca caacgccgac
     9121 gccggcctgc gtttccagtt ggtggatcgc catccctggc agttcagcgg tgagggcttc
     9181 cggctgggcc ggatgcagtt gctgcttgcg acgccgctgc tgctggcggc gatggtggtc
     9241 gcggcctggc gcggcatgcg cgatgcgcgt ccgggcgtgc gctacctgtc gctctccggc
     9301 gcgatgatcg tgctcggctt ccttgcgctg ggcttcttcg ccgatcgcga gcgcgccagc
     9361 ttccactggc ccttgccggg ctacatcgcc ttgctgccgc tggtgcccgc ggtgcttgga
     9421 cgctggcatc gcggctggcg catcgccgca tggaccatcg cggcgctcgg cctggcgacg
     9481 gtactcgcgt tctacgtggc cgcgtcggtg ccggcgctgc gcgagcaggc ggcatcgcgc
     9541 aatttccatc ccaccaattt cgccggttgg gacgtgctgg cgcgcgaggt cgatgcccgt
     9601 ctcgcgacga tgcccgaggg cacgcgcgtg ctggccggca atttcaagat cggcgcggag
     9661 ctgggcttcg cccgcggcga cgccgacatc gccgtgctcg accacccgct caaccacaag
     9721 cacggacgcg cgccgcagct tgcgctgtgg ggactcgcgc acgacacccg cgactcgctt
     9781 ggcgacacgc cgctattgct ggtcgccagc agcaacgacg tcgatttccg cgacctgctc
     9841 gcgcattacc accggctgtg cgagcaggtc gggccgctgc cgccgccgcg cgtgctcaac
     9901 atcgaccatg gccgccagcg cttcctgctg ttcgagctgg ggacgagccg gcgcgatggt
     9961 ccgtgcacca cgccggcgat cgcgttcctg gatgcgcccg cgccatcggc gcgcgtcggc
    10021 ggtgccttcc aggtggccgg ctgggcgttc aaggatggcg tgggcctgcg cggtgtcgat
    10081 gtgctggtcg atggccacgt cgttgcgcat gcacgctatg gcgaggcgaa cacgggcgtg
    10141 gccgatttct ggagggggag caccgatccc aatcatccgc gcgtgtactt ccgtgcgcag
    10201 gttgccggcc tcccggctgg ccggcactgg ctgggcctgc gcctgcatgg ggccgatggc
    10261 agcagcgagg actggccgtc gcagctcgtc gtggtgcgct aggggaggac gaagccggcc
    10321 tggcgcagca accggcaggt cgcgatcagc ggcaagccga ccagggcgct cgggtcgcgg
    10381 ttgtcgatcg cctcgaacag ggtgatgccc aggccctccg acttgaagct gccagcgcag
    10441 tcgtagggct gctcggcatc gacgtagcgc tcgatcgcag catcgtccag caggcggaac
    10501 tgcacctcgg tcaggtcgat gtcggtgaac acccggccgt ccgcgtgggc gaggcacaac
    10561 gcggtatgga aacgcacggt gcgcccggac atcgtccgta gttgcccgat cgccgcgtcg
    10621 cgtccgcccg gtttgcccaa gggcacgcca tcgaggtcgg cgacctggtc ggaacccaac
    10681 gcccacgcat cgtccggcag gccgtgcgcc acgtcgcggg ccttggcaac ggccaggcgg
    10741 ttcgccagtg cgcgtgggga ctcggcgggc agcggcgttt cgtcgac
//
LOCUS       DAOOCX010000080        10549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5916_length_10549_cov_8.455975, whole genome shotgun sequence.
ACCESSION   DAOOCX010000080 DAOOCX010000000
VERSION     DAOOCX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5916_length_10549_cov_8.455975"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..938
                     /locus_tag="PK399_08415"
     CDS             144..938
                     /locus_tag="PK399_08415"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPW13180.1"
                     /translation="MKKLLLVAGACLLLSGPALAQRNKNDPKGAAAGANSSGASATLE
                     HCDAPLGTVGVVEEQDGDWYRYLTSDLRLPSTIPLIRMMIQQSNCFVVVERGRAMQNM
                     MQERALADSGEIREGSNFGKGQMVAADYTINPTINFSQSDAGGIGGVLGAFGGRKLGA
                     IGAVVGGLKFKKAETILTMIDNRSGVQLAAAQGQARKTDFGGGIAGWGGSGYGALGGY
                     TNTPEGKVLAAAFADAYNNLVRALRNYEAQEVEGGLGKGGKLKVGN"
     gene            1026..1250
                     /locus_tag="PK399_08420"
     CDS             1026..1250
                     /locus_tag="PK399_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPW13181.1"
                     /translation="MPTLLVKAKPNARLSSLTRQDDGTWLAQLKSAPVDGKANAELIG
                     LVARHFGCAKSSVSIRTGAGSKLKRVEIPD"
     gene            complement(1263..1775)
                     /locus_tag="PK399_08425"
     CDS             complement(1263..1775)
                     /locus_tag="PK399_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13182.1"
                     /translation="MLRPHILLSLLLVATASVQAADPVPEIRRDVASPQATGVVHTLR
                     TIPEACARLEGRFTGNAGKPYEFAAVRTSDRCAPRAKLVDAANAKASVANGWVLNDVI
                     RVPSASCPSRQAVVRVWRKDAKVAPPKLDAQGRSRIYLKDSMDAARAGDLKPIPVFAA
                     AMTLEGLACK"
     gene            1966..4200
                     /locus_tag="PK399_08430"
     CDS             1966..4200
                     /locus_tag="PK399_08430"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPW13183.1"
                     /translation="MLEQYGLVLALVCAVIAIAYGLWAAGWINRQPAGNERMQEIAGA
                     IQEGARAYLNRQYMTISVAGVVLFVLIGVFLNWHTAIGFAIGAILSGAAGYIGMNVSV
                     RANVRTAEAARSGMGPAMNVAFKGGAITGMLVVGLGLLGVAGYYFVLQKMGLSQADNL
                     HALVGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYAVTVIATMLLGGMMTMVPGSADFAGKNAVLYPLVLGGVSIL
                     ASIIGAAFVKVSGDKPNIMGALYKGVIISAVLAAIAFYPITTQLMGDNAHGAMNLYIC
                     ALIGLLLTGVIVWITEYYTGTQYKPVQHVAKASTTGHGTNIIAGLGVSMKSTALPVIA
                     VCLAIWFSYLHGGLYGIAIAATSMLSMAGMIVALDAYGPITDNAGGIAEMAELPPEVR
                     NITDPLDAVGNTTKAVTKGYAIGSAALAALVLFADYTHNLEAANGGMKFAFDLSDHRV
                     IIGLLIGGLIPYLFGAMAMEAVGRAAGSVVEEVRRQFRDIPGIMDGTGKPQYDKAVDM
                     LTKSAIKEMIVPSLLPVAVPIIIGLLLGPEALGGLLIGTIVTGIFVAISMTTGGGAWD
                     NAKKYIEDGNFGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALLIVPLL
                     PVSSASGDNHATAAPVAVEAAAVEAAPAPAVEAPAPVAEAPATAEGATAAPADAPVAE
                     VPAQ"
     gene            4297..5343
                     /locus_tag="PK399_08435"
     CDS             4297..5343
                     /locus_tag="PK399_08435"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPW13184.1"
                     /translation="MRRACLSLLAALMLAPCAPAAIAQAKPPAATQVTLAPGQFLWHP
                     EIAPSGPMVLVISLDEQRAYVYRNGLAIGVSTISSGKPGKETPTGVFTILQKNKDHRS
                     NLYSNAPMPWMQRLTWDGIALHGGALPGYPASHGCVRLPHAFAEKLFAETRTGDTVVV
                     ANAKAGAASAVYPAVLAPVAKDGSAVSTGNILAGYTWNDALTNPTGPVSVLVSLADKR
                     VFAMRNGVVFAEAALDIRDGFAIGGSVLIVIGGENTAGPSLLAPDRPRHDWTAYPILG
                     VDGESLAMLQLQLQSMPLRVDDAFARRLYDVLKPGTTLLLTDLPAVRPDPSAALQPVL
                     ESDDATPAGGAPNR"
     gene            5439..5987
                     /gene="ppa"
                     /locus_tag="PK399_08440"
     CDS             5439..5987
                     /gene="ppa"
                     /locus_tag="PK399_08440"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPW13185.1"
                     /translation="MGLDLVPTGKNPPDELNVIIEIPKDAEPVKYEVDKASGAIFVDR
                     VLSTPMRYPCNYGYVPHTLCGDGDPADVLVLMPLALIPGSVIRVRPVGVLRMVDEAGA
                     DEKMLAVPISKVYPGYDHIQHIDDVPKHWLDRIGYFFEHYKDLEAGKWVKLDGWGGRD
                     EARQLLVESIERYEASAEKPNF"
     gene            complement(6030..6965)
                     /locus_tag="PK399_08445"
     CDS             complement(6030..6965)
                     /locus_tag="PK399_08445"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HPW13186.1"
                     /translation="MKLPRPFIQLPLLFDAGVLAAEIEALGEGVWRDHPQKFPGNSML
                     PLLAVDGEPANESFAGTMAPTPELLRCPYLMQVMASFGATLGRTRLMRLAGQAEVTPH
                     VDQGYYWAERVRVHVPIVTQPTVRFECGEVAIHMAAGECWIFDTWRNHNVINDASQSR
                     IHLVADTVGGEAFWDLVAAGRGHDADRGAWKAIHVPPSSAAAALVCEHYNIPRAMTPW
                     EADYHMRRLLDDADPRDPNLAAAHAQVERFFQAWRGLWARYGDSGEGKAEYWATLERF
                     IQQVPEPVAMIRLANGTRWIDGVLARVAKMAVRRS"
     gene            complement(7027..7716)
                     /locus_tag="PK399_08450"
     CDS             complement(7027..7716)
                     /locus_tag="PK399_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HPW13187.1"
                     /translation="MSVEDVALQTQRLRRVARRHPSAFLLAAQLLSLLVYPLMDDGTG
                     GRMLFGAVALVVVPLAVWVVNRSSFVNTVAWLLAIPAMLLTVIAVAFGNDALLPYSAL
                     LEAALYFYAAASLISYMLHDHKVTADELFAAAATFTLLAWGFAYAYYVCQAWYPGSFT
                     GFEPGRPRTWMELLFYSFSNLSATGLGDVLPVSAPARVLTMLEQFAGVGYIATVVSRL
                     IGLTIVRQERG"
     gene            complement(7747..8328)
                     /locus_tag="PK399_08455"
     CDS             complement(7747..8328)
                     /locus_tag="PK399_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HPW13188.1"
                     /translation="MRALQRTSQWLFVLLATAMLAACATGPQVRTDQDPTADFSKYRT
                     WSFYSPIAMEESGYSTWISDRIKDNVRKEMEARGYRYVESSPDLQVNFQGVVQDRTDV
                     FSMPRSGVQFFYNYRRNAYVGVPVWYDETQVSRYREGTLTVDLVDARRNRMVWTGDAI
                     ARVVKRTPEQKMAEIDQAINAIFLQYPHRAGTP"
     gene            complement(8437..10323)
                     /gene="thiC"
                     /locus_tag="PK399_08460"
     CDS             complement(8437..10323)
                     /gene="thiC"
                     /locus_tag="PK399_08460"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPW13189.1"
                     /translation="MNAIPKPAADLLQQTGQLSESVTRPIPGSRKIFVEGSRPDLRVA
                     MREIAQTQTPTLFGGETNPPITVYDPSGPYTDPDARIDLSVGLPALRAKWIEERGDTE
                     LLPQLSSAFGRGREHDPKLAGVRFPNRTLPRVAKAGANVSQMHYARKGIVTPEMEYVA
                     IRENQRLDAVRDALLLKQHPGESFGASIQKFITPEFVRDEIARGRAILPNNINHPESE
                     PMIIGRNFLTKINANIGNSAVSSGIAEEVEKLVWSIRWGGDTVMDLSTGKHIHETREW
                     IIRNSPVPIGTVPIYQALEKVDGRAEELNWEIFRDTLIEQAEQGVDYFTIHAGVLLRH
                     VPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICEIMKAYDVAFSLGDGLRP
                     GCIADANDAAQFGELEALGELTKIAWKHDVQTMIEGPGHVPMQMIKENMDKQLAECGE
                     APFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNREDVRDGI
                     MAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWEDQFHLGLDPEKAKSFHDETLPK
                     EAHKVAHFCSMCGPHFCSMKITQDVRDYAAEHGVDEAVALEAGMAEKSAEFREQGAEV
                     YREA"
     regulatory      complement(10429..10534)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1745 a         3566 c         3564 g         1674 t
ORIGIN      
        1 ggccggaacg gactgcccga ttacgtagta ctcccgcaag ccacgatacg gcgcaaactg
       61 cccgcctggg ccggctgagc accggggcag atccggccga gccgccgttt cgaacgatcc
      121 agacacaacc tgaggggata tcgatgaaga agctgttgtt ggtcgcgggc gcctgcctgc
      181 tgttgagcgg accggccctg gcccagcgca acaagaacga cccgaagggc gccgcggccg
      241 gcgccaattc gtcgggggct tccgcaacgc tcgagcactg cgacgcgccg ctgggcacgg
      301 tcggcgtggt cgaggagcag gacggcgact ggtatcgcta cctgacctcc gacctgcgcc
      361 tgccgtccac gattccgctc atccggatga tgatccagca gtccaactgc ttcgtggtgg
      421 tcgagcgtgg ccgcgcgatg cagaacatga tgcaggaacg cgccctggcc gacagcggcg
      481 agatccgcga gggcagcaac ttcggcaagg gccagatggt cgccgccgac tacaccatca
      541 atcccaccat caacttctcg cagagcgatg ccggcggcat cggcggggtg ctcggtgcct
      601 tcggtggccg caagctgggt gcgatcggcg cggtggtcgg cgggctgaag ttcaagaaag
      661 cggaaaccat cctgaccatg atcgacaacc gctccggcgt gcagctggcg gcggcgcagg
      721 gccaggcgcg caagaccgac ttcggcggcg gtatcgccgg ctggggcggt tcgggctacg
      781 gcgcgctggg cggctacacg aataccccgg aaggcaaggt gctcgccgcg gccttcgcgg
      841 atgcctacaa caacctggtg cgcgccctgc gcaactacga ggcacaggaa gtcgaaggcg
      901 gcttgggcaa gggcggcaag ctgaaagtcg gcaactgagt ccctgccccg caccaccccg
      961 ggacgcccgg catcgccggg cgttcctgtt tccgcggacc ccgcatcgca tgccatgctg
     1021 ccgcgatgcc gaccctgctg gtgaaagcca aaccgaatgc ccgcctctcc tcgctcacgc
     1081 gccaggacga cggcacctgg ctggcgcagc tgaaatcggc gccggtggat ggcaaggcga
     1141 atgcggaact gatcgggttg gtcgctcgcc atttcggctg cgccaaatcc tcggtgagca
     1201 tcaggaccgg cgcgggcagc aagctcaagc gggtggaaat cccggactga cgcgggccgc
     1261 aatcacttgc aggccaggcc ttccagcgtc atcgccgcgg cgaacaccgg gattggcttg
     1321 aggtcgccgg cgcgggcggc gtccatgctg tccttgaggt agatccgcga acggccctgt
     1381 gcatccaact tgggcggggc caccttcgca tccttgcgcc agacgcgcac caccgcctgg
     1441 cgcgacgggc agctcgcgct cggcacgcgg atcacgtcgt tcaggaccca gccgttggcg
     1501 accgaggcct tggcattggc ggcatcgacc agcttcgcgc gcggggcgca acgatcgctg
     1561 gtccggaccg cggcgaactc gtagggcttg ccggcattgc cggtgaaccg gccctccagg
     1621 cgcgcgcagg cttcggggat ggtgcgcaac gtatggacga cgccggtggc ttgcgggctg
     1681 gccacgtcgc gacggatttc gggcaccgga tcggcagcct ggacgctggc ggtggccacc
     1741 agcaacaggc tcaacagtat gtgcggtcgc agcatcgcat ggctccggtg ggacggctgg
     1801 gagcggcagg ctgccacgcg ttcgatgaat cgggcattca tggcgcgatc cctgcttccg
     1861 cgcgcatccg gcaggcgccg cgctgtgcga tagtcggggc gtcgcccgcc gacggcgggt
     1921 gcacacccaa cggtcttcca tacgaacact ccggggaggg gtttcatgct ggagcaatac
     1981 ggtttggtat tggcgctggt ctgcgccgtc atcgcgatcg cctacggcct gtgggccgcc
     2041 ggctggatca accggcaacc cgccggcaac gaacgcatgc aggagatcgc gggggcgatc
     2101 caggaaggcg cgcgcgccta cctcaatcgc cagtacatga cgatctcggt cgccggcgtg
     2161 gtcctgttcg tcctcatcgg cgtgttcctg aactggcata ccgcgatcgg cttcgccatc
     2221 ggcgcgatcc tgtccggcgc cgccggctac atcggcatga acgtgtcggt gcgcgccaac
     2281 gtgcgcaccg ccgaggccgc gcgcagcggc atgggcccgg cgatgaacgt cgccttcaag
     2341 ggcggcgcga tcaccggcat gctggtggtg gggctgggcc tgctgggcgt ggccggctac
     2401 tacttcgtgc tgcagaagat gggcctgtcg caggccgaca acctgcacgc gctggtgggc
     2461 ctggcgttcg gctcgtctct gatctcgatc ttcgcgcgcc tcggcggcgg catcttcacc
     2521 aagggtgcgg acgtcggtgc cgacctggtc ggcaaggtcg aggccgggat tcccgaagac
     2581 gacccgcgca acccggcggt gatcgccgac aacgtgggcg acaacgtcgg cgactgcgcc
     2641 ggcatggccg ccgacctgtt cgagacctac gcggtcacgg tgatcgccac catgctgctg
     2701 ggcggcatga tgacgatggt gccgggcagc gcggacttcg ccggcaagaa cgcggtgctg
     2761 tatccgctgg tgctgggcgg cgtgtcgatc ctcgcatcga tcatcggcgc ggccttcgtc
     2821 aaggtcagcg gcgacaagcc caacatcatg ggcgcgctgt acaagggcgt gatcatctcg
     2881 gcggtgctgg cggcgatcgc gttctacccg atcaccaccc agctgatggg cgacaacgcc
     2941 cacggcgcga tgaacctcta catctgcgcg ctgatcggcc tgctgctgac cggcgtgatc
     3001 gtgtggatca ccgagtacta caccggcacc cagtacaagc cggtgcagca cgtggccaag
     3061 gcctccacca ccgggcacgg caccaacatc atcgccggcc tcggcgtgtc gatgaagtcc
     3121 accgcgctgc cggtgatcgc ggtctgcctg gcgatctggt tctcctacct gcatggcggc
     3181 ctgtacggca tcgccatcgc cgccacgtcg atgctgtcga tggctggcat gatcgtggcg
     3241 ctggacgcct acggcccgat caccgacaac gccggcggca tcgccgagat ggcggagctg
     3301 ccgccggaag tgcgcaatat caccgacccg ctggacgcgg tgggcaacac caccaaggcg
     3361 gtgaccaagg gttacgcgat cggctcggcg gcgcttgcgg cactggtcct gttcgccgac
     3421 tacacgcaca acctggaagc ggccaacggc ggcatgaagt tcgcgttcga cctgtccgac
     3481 caccgggtga tcatcggcct gctgatcggc ggcctgatcc cgtacctgtt cggcgccatg
     3541 gcgatggaag ccgtgggccg cgcggccggc agcgtggtgg aggaagtgcg ccgccagttc
     3601 cgcgacatcc ccgggatcat ggacggcacc ggcaagccgc agtacgacaa ggccgtggac
     3661 atgctgacca agtccgcgat caaggaaatg atcgtgccgt cgctgctgcc ggtggcggtg
     3721 ccgatcatca tcggcttgct gctggggccg gaggcgcttg gcggcctgct gatcggcacc
     3781 atcgtgaccg gcatcttcgt ggcgatctcg atgaccaccg gtggcggcgc gtgggacaac
     3841 gccaagaagt acatcgagga tggcaacttc ggcggcaagg gctccgaggc gcacaaggcc
     3901 gcggtgaccg gcgacaccgt cggtgatccg tacaaggaca ccgccggccc ggcggtcaac
     3961 ccgctgatca agatcatcaa catcgtggcc ctgctgatcg tgccgctgtt gccggtctcc
     4021 agcgccagcg gcgacaacca tgcgacggct gccccggtgg cggtcgaggc cgcggcggtg
     4081 gaggcagcgc ctgccccagc cgtcgaggcc cctgcgccgg ttgccgaagc gccggcaacg
     4141 gccgaaggcg cgaccgccgc cccggccgat gcgccagtgg cggaagtgcc ggcgcagtaa
     4201 cgagccggcg gggaatccac ggaacggcgg cttcggccgc cgttttcgtt tccggcatcc
     4261 gccatactcc gcggcgtccc cgccggtgga acccccatgc gccgcgcctg cctctcgctg
     4321 ctcgccgccc tcatgctcgc gccctgcgcg cccgccgcaa tcgcgcaagc caagccaccc
     4381 gccgccacgc aggtcacgct ggcgccgggc cagttcctgt ggcacccgga gatcgcgccc
     4441 agcgggccga tggtgctggt gatcagcctg gacgagcaac gcgcctacgt gtaccgcaac
     4501 ggcctggcga tcggcgtgtc cacgatcagt tcgggcaagc cgggcaagga aacgcccacc
     4561 ggcgtgttca ccatcctgca gaagaacaag gaccaccggt ccaacctcta cagcaacgcg
     4621 ccgatgccgt ggatgcagcg actgacctgg gacggcatcg ccttgcatgg cggggcgctg
     4681 ccgggttacc cggcctcgca cggctgcgtt cgcctgccgc acgccttcgc ggaaaagctc
     4741 ttcgcggaaa cccgcaccgg cgacaccgtg gtggtagcga atgccaaggc cggtgccgcc
     4801 agcgcggtgt acccggcggt actggcgccg gtggcgaaag acggctcggc ggtctcgacc
     4861 ggcaacatcc tggccggtta cacctggaac gacgccctca ccaacccgac cggcccggtc
     4921 agcgtgctgg tcagcctggc ggacaagcgc gtgttcgcga tgcgcaacgg cgtggtgttc
     4981 gccgaagccg cgctggacat ccgcgacggc ttcgccatcg gcggctcggt gctgatcgtg
     5041 atcggcggcg agaacaccgc tggtcccagc ctgctcgccc ccgaccgccc acgccacgac
     5101 tggaccgcat acccgatcct gggcgtggac ggcgaatcgc tggccatgct gcaactgcag
     5161 ttgcaatcga tgccgctgcg ggtggatgac gccttcgcgc gccgcctcta cgacgtcctc
     5221 aagcccggca ccacgctgct gctcaccgac ctgccggcgg tgcggccgga tccttccgca
     5281 gcgctgcagc cggtgctgga atccgacgac gccacgcccg ccggcggcgc accgaaccgg
     5341 taaccgatcg cggcgtgctg caacgcagca ccggccgacg tagaatgccg ggccagagcg
     5401 gccgaacggc cccacccacc tccaggaacg accacgtcat gggcctcgac ctcgtcccca
     5461 ccggcaagaa tccgccggac gaactcaacg tcatcatcga aatcccgaag gatgccgagc
     5521 cggtcaagta cgaagtggac aaggccagcg gggcgatctt cgtcgaccgc gtgctgtcca
     5581 cgccgatgcg ctacccgtgc aactacggct acgtcccgca cacgctgtgc ggcgatggcg
     5641 acccggccga cgtgctggtg ctgatgccgc tggcgctcat ccccggctcg gtgatccggg
     5701 tgcgcccggt tggcgtgctg cgcatggtcg acgaggccgg cgccgacgag aagatgctgg
     5761 cggtgccgat cagcaaggtc taccccggct acgaccacat ccagcacatc gacgacgtgc
     5821 cgaagcactg gctcgaccgc atcggctact tcttcgagca ctacaaggac ctggaagccg
     5881 gcaagtgggt caagctggac ggctggggcg gccgcgacga ggcccgccag ctgctggtcg
     5941 agtcgatcga gcgctacgaa gccagcgcgg aaaagccgaa cttctgagcc aggccgcatc
     6001 gccccgcagt gcatgcgggg cgggcgcggt caggatcgcc tgaccgccat cttggccacg
     6061 cgcgccagca cgccatcgat ccagcgcgtg ccattggcca gcctgatcat cgcgaccggc
     6121 tccggcacct gctggatgaa ccgttccagc gtcgcccagt attccgcctt accttcgccc
     6181 gaatcgccgt agcgggccca caggcctcgc caggcctgga agaagcgctc gacctgcgca
     6241 tgcgcggccg ccaggttcgg atcgcgcgga tcggcatcgt ccagcaggcg gcgcatgtgg
     6301 tagtccgcct cccatggtgt catcgcccgg gggatgttgt agtgctcgca caccagcgct
     6361 gccgccgccg atgaaggggg gacatggatc gccttccacg ctccgcggtc ggcgtcgtgt
     6421 ccccgccccg cggcgaccag atcccagaac gcttcgccgc cgacggtatc ggcgaccagg
     6481 tggatgcggc tctggctggc atcgttgatg acgttgtggt tgcgccacgt atcgaagatc
     6541 cagcactcgc ccgcggccat gtggatggcc acctccccgc actcgaaacg caccgtcggc
     6601 tgggtcacga tcggcacgtg cacgcgcacg cgctcggccc agtaatagcc ctggtccacg
     6661 tgcggggtga cttcggcctg cccggccagg cgcatcaggc gcgtgcgccc gagggtggcg
     6721 ccgaaactgg ccatcacttg catcaggtag gggcagcgca gcagttcggg cgtcggcgcc
     6781 atggtgccgg cgaaggattc attggccggc tcgccgtcga cggccaacaa gggcagcatc
     6841 gaattgccgg ggaatttctg cgggtggtcg cgccatacgc cttcgcccag cgcctcgatc
     6901 tcggccgcga gcacgccggc gtcgaacagc agcggcaact ggatgaacgg acgtggcagc
     6961 ttcattccgg tggatccggg cgatgcatcg gcgaggccgc cattgtagcg gccccacgct
     7021 gcggcttcag ccgcgctcct ggcggacgat ggtcaacccg atcagccgcg agaccacggt
     7081 ggcgatgtag cccacgccgg cgaactgctc gagcatggtc agcacgcgtg ccggcgcgct
     7141 gaccggcaac acgtcgccca ggccggtcgc cgagaggttg ctgaagctgt agaacagcag
     7201 ctccatccag gtgcgtgggc gccccggctc gaacccggtg aaactgccgg gataccaggc
     7261 ttggcagacg taatacgcgt atgcaaagcc ccaggccagc aaggtgaacg tggcggccgc
     7321 cgcgaacagt tcgtcggcgg tcaccttgtg gtcgtgcaac atgtaggaga tcaggctggc
     7381 cgcggcgtag aaatacagcg ccgcttccag caatgccgaa tagggcagca gtgcatcgtt
     7441 gccgaaggcc acggcgatca cggtcagcag catcgccggg atcgccagca gccaggccac
     7501 cgtgttgacg aacgaactgc gattgaccac ccacaccgcc agcggaacca ccaccagggc
     7561 gaccgcgccg aacagcatgc ggccgcccgt gccgtcgtcc atcaacggat agaccagcag
     7621 gctgagcagt tgcgccgcca gcaggaacgc cgacggatgg cggcgcgcga cgcggcgcag
     7681 gcgctgcgtc tgcagggcga catcctcgac cgacatggcg gttgcttcag gcgatgcggg
     7741 aagcgatcag ggcgtgcccg cgcgatgcgg gtactgcagg aagatcgcat tgatggcctg
     7801 gtcgatctcg gccatcttct gctcgggcgt gcgcttgacc acccgggcga tcgcatcgcc
     7861 ggtccagacc atgcggttgc ggcgcgcatc gaccaagtcg acggtcaggg tgccctcgcg
     7921 gtagcggctg acctgggtct cgtcgtacca gaccggcacc ccgacatagg cattgcgccg
     7981 gtagttgtag aagaactgca cgccgctgcg cggcatcgag aacacatccg tccgatcctg
     8041 caccacgccc tggaaattca cctgcaggtc gggggacgac tcgacgtagc gataaccgcg
     8101 cgcctccatt tccttgcgca cgttgtcctt gatccggtcg gagatccagg tggaataacc
     8161 cgactcttcc atcgcgatcg ggctgtagaa cgaccaggtc cggtacttgc tgaagtccgc
     8221 cgttggatcc tggtcggtcc gcacctgcgg accggtggca catgccgcca gcatggccgt
     8281 ggcgaggagg acgaacaacc attggctggt gcgctgcagg gctcgcatgg aagcactccc
     8341 ggggtcgatg cggacgagcc ggaacactag catgcagccc tgcaacgcga ggcgaacgcg
     8401 accagccaaa ccatgaacgg gctgcggtcc gatggatcag gcctcgcggt agacctcggc
     8461 accctgctcg cggaactccg cggatttctc cgccatgccg gcctccagcg caacggcttc
     8521 gtccacgcca tgctcggcgg catagtcgcg cacgtcctgg gtgatcttca tcgaacagaa
     8581 atgcgggccg cacatcgagc agaaatgcgc gaccttgtgc gcctccttcg gcagggtttc
     8641 gtcgtggaag gacttcgcct tctccggatc caggcccagg tggaactggt cttcccaacg
     8701 gaattcgaaa cgcgccttgg agagcgcgtt gtcgcgcacc tgcgcacccg gatggccctt
     8761 ggcgaggtcg gccgcgtgcg ccgcgatctt gtaggccatg atgccgtcgc gcacgtcttc
     8821 gcgattcggc aggccaaggt gttccttcgg cgtcacgtag cagagcatcg cggtgccgaa
     8881 ccacccgatc atcgccgcgc cgatcgcgga ggtgatgtgg tcgtagcctg gcgcgatgtc
     8941 ggtggtcagc ggcccgagcg tgtagaacgg cgcctcgccg cactcggcca gctgcttgtc
     9001 catgttctcc ttgatcatct gcatcggcac gtggccgggg ccttcgatca tggtctgcac
     9061 gtcgtgcttc cacgcgatct tggtcagttc gcccagcgct tccagctcgc cgaactgggc
     9121 ggcgtcgttg gcgtcggcga tgcagccggg acgcaggccg tcgcccaggc tgaaggccac
     9181 gtcgtaggcc ttcatgatct cgcagatgtc ctcgaaatgg gtgtagagga aattctcctt
     9241 gtgatgcgcc aggcaccact tggccatgat cgaaccgccg cgcgacacga tgccggtgac
     9301 ccgcttcgcg gtcagcggca cgtgccgcag cagcacgccg gcatggatgg tgaagtagtc
     9361 cacgccctgc tctgcctgtt cgatcagggt gtcgcggaag atctcccagt tgagttcctc
     9421 ggcgcggccg tccaccttct ccagcgcctg gtagatcggc acggtgccga tcggcaccgg
     9481 cgaattgcgg atgatccact cgcgggtttc gtggatgtgc ttgccggtgc tgaggtccat
     9541 caccgtgtcg ccgccccagc ggatcgacca caccagcttt tccacttcct cggcgatgcc
     9601 ggaagacacc gcgctgttgc cgatgttcgc gttgatcttg gtcaggaagt tgcggccgat
     9661 gatcatcggc tcgctttccg ggtggttgat gttgttcggg aggatcgcgc ggccgcgggc
     9721 gatctcgtcg cgcacgaatt ccggggtgat gaatttctgg atgctggcac cgaacgattc
     9781 gcccggatgc tgcttcagca gcaacgcatc gcgcaccgca tccagccgct ggttctcgcg
     9841 gatcgcgacg tactccatct ccggcgtgac gatgcccttg cgcgcatagt gcatctggct
     9901 gacgttggcg ccggccttgg ccacacgcgg caaggtgcgg ttgggaaagc gcacgccggc
     9961 cagcttcgga tcgtgctcgc ggccgcgacc gaacgccgag ctcagctgcg gcagcagttc
    10021 ggtgtcgccg cgttcctcga tccacttggc gcgcaatgca ggcaggccga ccgacaggtc
    10081 gatgcgggcg tcgggatcgg tgtacggacc ggacggatcg tagacggtga tcggcggatt
    10141 ggtttcaccc ccgaacaggg tcggcgtctg ggtctgcgcg atctcgcgca tcgccacccg
    10201 caggtcgggc cgcgagcctt cgacgaagat cttgcgcgag ccggggatcg gccgggtcac
    10261 ggattcggac aactgcccgg tctgctgcag caggtcggcg gcgggcttgg gaatggcatt
    10321 catcggcatc gtccagggtg gctcagggga cgaagcggcg gcgcaggaga ttgaatgtgg
    10381 gaggggcttc agccccgacg ccggggcagt cggggctgaa gcccctccca cattgcctgc
    10441 gaagcttccc tacgccggtc tgaaccggat caggttcgaa gggtctgtct caaccgccgg
    10501 gccgaagcgc cccgcggtac ccccgcttca gggcgaatta gaccacagc
//
LOCUS       DAOOCX010000081        10504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5962_length_10504_cov_9.517561, whole genome shotgun sequence.
ACCESSION   DAOOCX010000081 DAOOCX010000000
VERSION     DAOOCX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5962_length_10504_cov_9.517561"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PK399_08465"
     CDS             complement(<1..431)
                     /locus_tag="PK399_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPW13190.1"
                     /translation="MQTGERFHHMDNLRALAMLAGVVFHAALAYSPLMHPIWPLADAG
                     RSIAVDVVAWFLHLFRMPLFFVVAGFFAALLVARRGMAGLFRNRIARVLLPLVLFLPL
                     VLVSMHWLTMQAVANATHPSPALVWMRDWIEQHGALPSMPG"
     gene            complement(442..1101)
                     /locus_tag="PK399_08470"
     CDS             complement(442..1101)
                     /locus_tag="PK399_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HPW13191.1"
                     /translation="MVGKLIAKDWQVYQKQLAGFVAGLLLALALVGMGTPLLAAAGSL
                     LLLVLLLVVGSYAIQSSVMLERKEQTQPFVMSLPVTPMDVFWGKLLANLVIFLVPFLL
                     VSGGLLALVLTTPKPDGAVPWVIVVALFMLANFCVSLCVVIAVDSEGWNVFAMLALMT
                     LIGPFLYWVTWMPGIREHLQGDAIVWSPQVLALIGGELAVIGIGILIAGWLHARKASF
                     L"
     gene            complement(1140..2024)
                     /locus_tag="PK399_08475"
     CDS             complement(1140..2024)
                     /locus_tag="PK399_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW13192.1"
                     /translation="MTPAIALTGVRKRYDDFALHDIDLTLPRGQVMGLVGVNGAGKST
                     LLRLLMGLIRADGGEVEVLGQRLPQAQVAVKREIGYASDDMRLYRGQTLRWHMDFIRS
                     VYPEWDEAYAQTLLKRFDLKPQQALRGFSHGQRVKALLLLIFARHPRLLLLDEPTTGL
                     DPVARGEVLDAIADVLRDEERSILFSSHNTADIEQIADSITFLHGGKVVASRDKESFL
                     DNWRRIVCRGAMTDAVRALPGIASVRDSTPLVELKTGAFDASTLPALQALGLAVESVE
                     PMPLEDIFVTTVRGSHNA"
     gene            complement(2021..2449)
                     /locus_tag="PK399_08480"
     CDS             complement(2021..2449)
                     /locus_tag="PK399_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPW13193.1"
                     /translation="MRNELLLSPHSSVPMYAQIVEQVVAKVMSGAWQAGAPLPSIREL
                     ASDCQVSVITVKRAYLELERSGVIVTRHGKGSFVADALDATRGLATAEFDSQLQGLLD
                     AAARLGMARNELLDRVGEAFDASPGASQSTTTSTDKGTTA"
     gene            complement(2587..4692)
                     /locus_tag="PK399_08485"
     CDS             complement(2587..4692)
                     /locus_tag="PK399_08485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HPW13194.1"
                     /translation="MHTSFRRAALALAILASLAACDRGTEPPKATTAPVAAEAPAAGT
                     GLGIDLAGMDKAVQPGDDFDAYANGTWAKTTEIPADRSSTGTFLKVLELAEKRNAELV
                     QGLETSNPAAGSNERKIADYYAAYMDEAGIEARGLAPLQAKLDSVAAITDTAGLSRWL
                     GASLRADTDPLNATNFYTENLFGLFVAQALEDPSKNVGYLMQGGLGMPDREYYVGADK
                     DMAANRAAYQAYIAAVLKQAGDADAENKAKAIFDLEMKIARAHANVVDSQDIHKANNP
                     WATADFAKKAPGIDWTAFFDAAKLSGQQTIFAWQPGAITALSGLVASEPLQTWKDYLH
                     FHAINHSAALLPKAYADLSFAFYGTQLQGTSQQRDRWKRALAATDGALGDAIGQLYVK
                     QYFPASSKSKVEGMVSNILAAFRDGVDKLAWMSPETKAIAKAKAETMLVGVGYPDSWR
                     DYSSLEVKRDDPVGNALRAEEAEYRHQTAKLAKAPDRKEWWMTPQTVNAVQLPLQNAM
                     NFPAAILEAPFFDPKADDAANYGAIGAVIGHEVSHGFDNLGSEFDGDGRLKNWWTKDD
                     AAHFKAATDRLVAQYNSYEALPGLKINGEQTLGENIADVAGLQAAYTAYHASLGGKPA
                     PVIDGLTGDERFFLAYAQAWRTKMRDAALRARVVGDGHAPGRWRALTVRNIDAWYETF
                     KVPGTAKLYLKPEDRVKIW"
     gene            4877..6094
                     /locus_tag="PK399_08490"
     CDS             4877..6094
                     /locus_tag="PK399_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW13195.1"
                     /translation="MKRPVIVAIACVALALAGWMLLQSRRGPLLAGYQVQARPLVQTV
                     VATGRVVAVSRAQVGSPVTGVVVERRVREGDVVAPGDVLAVLRADDLEAAVREAEAAL
                     AQLQQSTRPQAQAAQREAEARLAQASREVTRRRELFKRQLIARETLEQAVQAEAVARS
                     TAEQARLAASALGAGNPNEAAARARVASARALLAKTTIRAEVAGTVLTRNAEPGDLVQ
                     PGRVLFEIARAGATEVLVPLDEKNLEVLAVGQPAMCIADAYPARPFPATVGFIAPSVD
                     PQRGSVDVRLTVSPVPGFLRQDMTVSVNVETGRRARAIVAPNDAISALEGNRATVWRV
                     AGGRVERREVTLGLRGLTQTEITDGLEAGDWILADAQAVVEDGDRVRVAASALPGGAA
                     DSATRKELPAKLD"
     gene            6100..7302
                     /locus_tag="PK399_08495"
     CDS             6100..7302
                     /locus_tag="PK399_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW13196.1"
                     /translation="MWIEWTIATRFLREGKAQSTLILVGIAVGVAVIVFLTALITGLQ
                     GNIVNRTLGTQAHIKVMPTEEANRILPPAAGSVQLVLENKRAQRLRSINNWQQVRDVL
                     DTLPQVRAVSPVISGPAFARRGEALESVALVGIDAARYQRIIPIQDDVIAGVFRIGAG
                     DAVIGKQLASDLGLRVGDKLRLDGGQGRDSVVNVAGIFELGVRELDARYVYLDMKQAQ
                     SLLNLPGAATLIDVTVDDIFSAQVVAARIARLTGLKAESWMETNGQLMNALRSQSLST
                     QMISLFVALSVALGIASVLSVSVVQRTREIGILRAMGTTRPQMLRVFLVQGALFGLAG
                     SLLGGVSGYGLVGAFNTFGPKLFYIPVDPWLLVAASLLATVTGILSAAVPARRAARLD
                     PVEAIRHV"
     gene            7295..8020
                     /locus_tag="PK399_08500"
     CDS             7295..8020
                     /locus_tag="PK399_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW13197.1"
                     /translation="MSEHDAGQEVLRLQGLKKRYNVGKPTEVEVLHGLDLRLQRQDFA
                     ALVGASGSGKSTLLNLIGLLDQPTAGELFLLGEPTSAMDDARRTALRGSAIGFVFQFH
                     HLIQAFTALENVLMPLMVAGGKPTREQVERARGLLAEVGLAGLEQRKPDQLSGGQQQR
                     VAVARALVSRPALLLADEPTGNLDSTSAAEVFQLFRSFNREFGCAVLLVTHDPRLSAQ
                     CDRTVTLVDGRIASDTRASSPAG"
     gene            8107..9924
                     /locus_tag="PK399_08505"
     CDS             8107..9924
                     /locus_tag="PK399_08505"
                     /inference="COORDINATES: protein motif:HMM:NF021824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HPW13198.1"
                     /translation="MDAVHGLDLGQPPFDLLDDAGRARLAARVDMGYHPRGQVIIAAG
                     APSEHVVVLMKGQVHAFDLDGAGHEQRFADYGPGDIFGAWAVMAGRARHSYRADTDVV
                     SFLIPAGLFRTLLSEQPAFAAWFQEGLAVKGQLADRARGSELAELMVTQVGAAQLAPA
                     VRVPATMSIADASERLREERVDCLLVDDPAHPQPGVVTRTDLLEALTRARQPLEALVG
                     PLASRPLVAQQTTDVLFQALVTMAERHIERIAVRDGEAIVGTLGMAEVLSHYASHSHL
                     ISLRLARARSLDEVADTARGMTRLVRSLHAQGARMPALMELVSALNSRVMARLFELLV
                     PRELQGRMCLLVLGSEGRREQLLKTDQDNALVVDDDLDWPELDATMARFTEAMGEVGY
                     PPCPGGVMVSNPHWRMSATQWRARLDQWRSHYAAQAAMDLAITLDARPVAGNAALFAP
                     LDARLQALGGDERLLHAMAAAVLQFESPISMFGQLRQDERGVDLKRSGIFPIVHGLRC
                     LALKHGIAQRNSFARCEALVPAGGLDATLARDLAQTLAVLQRLRLDAQLATLDAGGVP
                     DNLVQPAALRRLDRELLRDALRVVKAFQQHVRVVFHLGD"
     gene            9926..>10504
                     /locus_tag="PK399_08510"
     CDS             9926..>10504
                     /locus_tag="PK399_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPW13199.1"
                     /translation="MHPLSRWWMRRRYGAGEWGGLFAPAPDGEWVSLDLETTGLDPAR
                     DHILSLAAVPVRDGRVGLSERFERRVHTARAFGIESIRHHRITPDEASTGEQVTDVVR
                     EFLHWLGPRRLLGYHLGFDLDMLRPHVRALAGFELPNPTTDLADEVAARQRAQRPDAP
                     VDLDLVHIATRLGVPMIGRHTALGDATTVGLCW"
BASE COUNT         1609 a         3573 c         3705 g         1617 t
ORIGIN      
        1 cagcccggca tggagggcag cgcgccgtgc tgttcgatcc aatcgcgcat ccacaccagt
       61 gcgggcgatg gatgcgtggc gttggcgacg gcctgcatcg tcaaccagtg catgctcacc
      121 aataccagcg gcaggaacag caccagcggg agcagcacgc gcgcaatgcg attgcggaac
      181 aggccggcca tgccgcgacg cgcgacgagc aatgccgcga agaaccccgc gacgacgaag
      241 aacagcggca tccggaacag gtgcaggaac cacgcgacga catcgaccgc gatcgaccgc
      301 ccggcgtcgg cgagcggcca gatcggatgc atcagcgggc tgtatgcaag cgccgcatgg
      361 aagaccacgc cggcgagcat cgccagcgcg cgcaggttgt ccatgtggtg gaaacgttcg
      421 ccggtctgca tcgcgtcgcg ctcagaggaa cgaggccttg cgcgcatgca gccagccggc
      481 aatgaggata ccgatgccga tcaccgccag ttcgccgccg atcagcgcca gcacctgtgg
      541 gctccagacg atggcatcgc cctgcaggtg ctcgcggatg cccggcatcc aggtgaccca
      601 gtagaggaag gggccgatca gggtcatcag cgccagcatc gcgaacacat tccagccttc
      661 ggaatccacc gcgatcacca cgcacagcga gacgcagaaa ttggccagca tgaacaacgc
      721 gaccacgatc acccaaggta ccgcgccatc cggtttgggc gtggtcagca ccagcgccaa
      781 caggccgccg ctgaccagca ggaacggcac caggaagatg accaggttgg ccagcagctt
      841 gccccagaac acgtccatcg gcgtgaccgg caggctcatg acgaagggct gggtctgctc
      901 cttgcgttcg agcatcaccg aggactggat ggcgtaggaa ccgaccacca gcagcaggac
      961 cagcaacagc agcgaaccgg ctgcggccag cagcggggtg cccatgccca ccagtgccag
     1021 cgccagcaga agaccggcga cgaaaccggc gagttgcttc tggtagacct gccagtcctt
     1081 ggcgatcaac ttgccgacca tcgcggcatc cgatgcaaac cagtttgtac cgaacccgtt
     1141 catgcgttgt ggctcccgcg cacggtggtg acgaagatgt cttccagcgg catcggttcc
     1201 accgattcca ccgccagtcc cagcgcctgc aatgcaggca gcgtcgatgc atcgaacgcg
     1261 ccggtcttga gttcgaccag tggcgtgctg tcgcgcaccg aggcgatgcc gggcagggcg
     1321 cgtaccgcat ccgtcatcgc gccgcggcag acgatgcgcc gccagttgtc gaggaacgat
     1381 tccttgtcgc gcgaggccac gaccttgccg ccgtgcagga aggtgatcga atcggcaatc
     1441 tgctcgatgt cggcggtgtt gtgcgaggaa aacaggatcg agcgttcctc gtcgcggagc
     1501 acgtcggcga tggcgtcgag cacttcgccg cgcgcgaccg gatcgagtcc ggtcgtgggc
     1561 tcgtccagca gcaacagccg cggatggcga gcgaagatca gcagcagcaa ggccttgacc
     1621 cgctgcccat gcgagaaccc gcgcagcgcc tgctgcggct tcaggtcgaa gcgcttgagc
     1681 agggtctgcg catacgcctc gtcccactcg ggatacacgc tgcggatgaa gtccatgtgc
     1741 cagcgcaggg tctgcccgcg atacaggcgc atgtcgtccg acgcatagcc aatctcgcgc
     1801 ttcaccgcga cctgcgcctg cggcaggcgc tggccgagca cttcgacctc gccaccatcg
     1861 gcgcggatga ggcccatcaa caggcgcagc agggtcgact tgccggcgcc gttcacgccg
     1921 accaggccca tcacctggcc gcgcggcaac gtgaggtcga tgtcgtgcag ggcgaagtcg
     1981 tcgtagcgct tgcgcacgcc ggtcagcgcg atggcgggag tcatgccgtc gttcccttgt
     2041 cggtcgatgt ggtggtggat tgcgatgcgc ctggcgatgc atcgaaggcc tcgcccacgc
     2101 ggtcgaggag ttcgttgcgg gccatcccga gtcttgcggc cgcatccagc agcccctgca
     2161 gttgcgaatc gaattccgcg gtggccaggc cgcgcgtcgc atcgagcgca tcggcgacga
     2221 acgaaccctt gccgtggcgg gtgacgatca ccccggaccg ctccagttcc aggtaggcgc
     2281 gcttcaccgt gatcacgctg acctggcagt cgctcgccag ctcgcggatc gacggcagcg
     2341 gagcgccggc ctgccatgcg ccggacatca ccttggcgac cacctgctcc acgatctgcg
     2401 cgtacatcgg cacgctgctg tgcggggaga ggagcaattc gttgcgcatg cagtgcccga
     2461 acaactgtat atagcgaata tatacagata atctcagttg tcaatcagcc acgaaccgca
     2521 cccgagcgca gccaaaagaa acgcccggac aggccgggcg tttcttttgc agcaagccgt
     2581 gacggcttac cagatcttca cccggtcctc gggcttgagg tacagcttcg cggtgccagg
     2641 caccttgaag gtctcgtacc aggcgtcgat gttgcgcacc gtgagtgcgc gccagcgacc
     2701 cggggcgtgg ccatcgccga ccacgcgggc gcgtagcgcg gcatcgcgca tcttggtgcg
     2761 ccatgcctgc gcataggcca ggaagaagcg ctcgtcgccg gtgaggccgt cgatcaccgg
     2821 tgccggcttg ccgcccagcg atgcgtggta ggcggtgtac gccgcctgca ggccggcgac
     2881 gtcggcgatg ttctcgccca gcgtctgttc gccgttgatc ttcagtcccg gcagtgcctc
     2941 gtagctgttg tactgcgcca ccagccggtc ggtggcggcc ttgaagtggg ccgcgtcgtc
     3001 cttggtccac cagttcttca ggcggccatc gccatcgaac tcggagccca ggttgtcgaa
     3061 gccgtggctg acctcgtggc cgatcaccgc gccgatcgca ccgtagttgg cggcatcgtc
     3121 ggccttggga tcgaagaacg gggcttccag gatcgcggcg gggaaattca tcgcgttctg
     3181 cagcggcaac tgcacggcgt tgaccgtctg cggcgtcatc caccattcct tgcggtccgg
     3241 cgccttggcg agcttggcgg tctggtggcg atactcggcc tcttccgcgc gcagcgcgtt
     3301 gccgaccgga tcatcgcgct tgacctccag cgaggagtag tcgcgccagc tgtcggggta
     3361 gcccacgccg accagcatgg tttccgcctt cgccttggct atcgccttgg tctccggcga
     3421 catccacgcc agcttgtcca ccccgtcgcg gaacgcggcc aggatgttgc tgaccatgcc
     3481 ctccaccttg gacttggacg atgccgggaa atactgcttc acgtacagct ggccgatggc
     3541 atcgcccagc gcaccgtcgg tggcggccag cgcgcgcttc cagcggtcgc gctgctggct
     3601 ggtgccctgc aactgggtgc catagaacgc gaagctgagg tccgcatacg ccttgggcag
     3661 cagcgccgcg ctgtggttga tcgcgtggaa gtgcaggtaa tccttccagg tctgcagcgg
     3721 ctcgctggcg accagcccgg acaacgctgt gatcgcgccc ggctgccagg cgaagatcgt
     3781 ctgctggccg gacaacttgg ccgcatcgaa gaacgcggtc cagtcgatgc ccggtgcctt
     3841 cttggcgaag tccgcggtcg cccacggatt gttggccttg tggatgtcct ggctgtcgac
     3901 cacgttggca tgcgccctgg cgatcttcat ctccaggtcg aagatcgcct tcgccttgtt
     3961 ctcggcatcg gcatcgccgg cctgcttgag cacggcggcg atgtacgcct ggtaggcggc
     4021 gcgattggcc gccatgtcct tgtcggcgcc cacgtagtac tcgcggtccg gcatgcccag
     4081 gccaccctgc atcaggtagc ccacgttctt cgacggatct tccagcgcct gcgccacgaa
     4141 caggccgaac aggttctcgg tgtagaaatt ggtggcgttg agcgggtcgg tgtccgcgcg
     4201 caggctggca ccgagccagc gcgacaggcc ggcggtgtcg gtgatcgcgg ccacgctgtc
     4261 cagcttggcc tgcagcggcg ccaggccacg cgcctcgatg ccggcttcgt ccatgtaggc
     4321 ggcgtagtag tcggcgatct tgcgctcgtt gctgccggcg gccggattgg atgtctccag
     4381 tccctgcacc aactccgcgt tgcgcttctc ggcaagctcg aggaccttca ggaaggtacc
     4441 ggtgctggaa cgatccgcgg ggatctcggt ggtcttcgcc cacgtgccat tggcataggc
     4501 gtcgaagtcg tcgccgggtt gcacggcctt gtccatcccg gccaggtcga tgcccaggcc
     4561 ggtgccggct gcgggcgcct cggcggcgac cggcgcggtc gtggccttcg ggggttcggt
     4621 gccgcggtcg caggcggcga gcgaggccag gatggccagc gccagggcgg cgcggcggaa
     4681 agacgtgtgc atggaggagc ctcgcgagga tgacctgcgc atcctgcgcc gattcggggc
     4741 aggggcatgt gcaggaggtc attgcggcgc ggtgtccgcc tgctgcatgc cgccctcgca
     4801 ttgtcccagg gccggggccg cgcaaagtcc gtggcaatgg gtatggtgtg ccgtcgcgcc
     4861 tgccccggag tcccccatga aacgccccgt gatcgttgcc attgcctgcg tggcgctggc
     4921 cttggcgggg tggatgctgc tgcagtcgcg gcgcggaccg ctcctggccg gttaccaggt
     4981 gcaggcgcgg ccgctggtgc agacggtggt ggccaccggg cgggtggtgg cggtgtcgcg
     5041 cgcgcaggtc ggcagcccgg tcaccggggt ggtggtcgag cgccgcgtgc gcgagggcga
     5101 cgtggtggcg ccgggcgacg tcctcgcggt gctgcgcgcg gatgacctgg aggccgcggt
     5161 gcgcgaggcc gaggcggcat tggcgcaact ccagcagagc acgcgcccgc aggcccaggc
     5221 cgcgcagcgc gaagcggagg cgcgcctggc gcaggccagc cgcgaagtca cgcgccggcg
     5281 cgagctgttc aagcgccagc tcatcgcccg cgaaaccctg gagcaggcgg tgcaggcgga
     5341 ggccgtggcc cgcagcacgg ccgaacaggc gcggctggcc gcgagcgcgc tgggggccgg
     5401 caatccgaac gaggccgcgg cgcgggcgcg cgtggccagc gcgcgcgcat tgctggccaa
     5461 gaccacgatc cgcgccgaag tcgccggcac cgtgctcacc cgcaacgcgg aacccggcga
     5521 cctggtgcag ccaggccgcg tgctgttcga gatcgcacgc gccggcgcca ccgaagtgct
     5581 ggtgccgctg gacgagaaga acctggaagt gctcgcggtc gggcaaccgg cgatgtgcat
     5641 cgccgatgcc tatccggcgc ggccgttccc ggccacggtc ggcttcatcg cgcccagcgt
     5701 cgacccgcag cgcggttcgg tggacgtccg cctcaccgtc agtccggtgc ccggcttcct
     5761 gcgccaggac atgaccgtct cggtcaacgt ggaaaccggg cgccgcgcac gcgccatcgt
     5821 cgcgcccaac gacgcgatct ccgcgctgga gggcaaccgc gccacggtct ggcgggttgc
     5881 cggggggcgc gtcgagcgac gcgaggtgac cctgggcctg cgcggactca cccagaccga
     5941 gatcaccgac ggcctggagg cgggtgactg gatcctggcg gatgcacagg ccgtggtgga
     6001 ggacggcgac cgggtgcgcg tggccgccag cgcgctgccg gggggcgcgg ccgattccgc
     6061 gacccgcaag gaactgccgg ccaagctgga ctgagcccga tgtggatcga gtggaccatc
     6121 gccaccaggt tcctgcgcga gggcaaggcg cagagcacgc tgatcctggt cgggatcgcg
     6181 gtgggcgtgg cggtgatcgt gttcctcacc gcgctgatca ccggcctgca gggcaacatc
     6241 gtcaatcgca ccctgggcac ccaggcgcac atcaaggtga tgcccaccga ggaagccaac
     6301 cgcatcctcc cgcccgcggc cggcagcgtg cagctggtgc tggagaacaa gcgcgcgcag
     6361 cgcctgcgct cgatcaacaa ctggcagcag gtgcgcgatg tgctggacac gctgccgcag
     6421 gtgcgggcgg tgtcgccggt gatctcgggt ccggcgttcg cgcgtcgtgg cgaggcgctg
     6481 gagtcggtgg cgctggtcgg catcgatgcc gcgcgctacc agcggatcat cccgatccag
     6541 gacgacgtga tcgccggcgt gttccggatc ggtgccggcg acgcggtgat cggcaagcag
     6601 ctcgcgtccg acctcggcct gcgggtgggc gacaagctgc gcctcgacgg cggccagggc
     6661 cgcgacagcg tggtcaacgt ggccggcatc ttcgaactcg gggtgcgcga actggatgcg
     6721 cgctatgtgt acctggacat gaaacaggcg cagtcgctgc tcaacctgcc gggcgccgcc
     6781 accctcatcg atgtcaccgt cgacgacatc ttctccgcgc aggtcgtggc ggcgcgcatc
     6841 gcccggctta ccgggctgaa ggcggaaagc tggatggaaa ccaacggcca gctgatgaac
     6901 gcgctgcgct cgcagagcct gtccacgcag atgatcagcc tgttcgtggc gctgagcgtg
     6961 gcgctgggca tcgccagcgt gctgtcggtg agcgtggtcc agcgcacccg ggagatcggg
     7021 atcctgcgcg cgatgggcac gacccgtccg cagatgctgc gcgtgttcct ggtgcagggt
     7081 gcgttgttcg ggctggccgg ctcactgctc ggcggagtgt cgggctatgg cctggtgggc
     7141 gcgttcaaca ccttcgggcc gaagctgttc tacatcccgg tcgacccgtg gctgctggtc
     7201 gccgcctcgt tgctggccac ggtcaccggc atcctgtcgg cggcggtgcc ggcgcggcgg
     7261 gcggcgcgcc tggacccggt ggaggcgatc cgccatgtct gagcacgacg ccggccagga
     7321 agtgctgcgc ctgcagggcc tgaagaagcg ctacaacgtg ggcaagccga ccgaggtgga
     7381 ggtgctgcat ggcctggacc tgcgcctgca gcgccaggac ttcgccgcgc tggtcggtgc
     7441 ctcgggctcc ggcaagagca cgctgctcaa cctgatcggg ttgctggacc aacccaccgc
     7501 gggcgaactg ttcctgctcg gggagccgac aagcgccatg gacgatgcgc gccgcacggc
     7561 attgcgcggc agtgcgatcg ggttcgtgtt ccagttccac cacctcatcc aggccttcac
     7621 cgcgctggaa aacgtgttga tgccgctgat ggtggccggt ggcaagccca cgcgggagca
     7681 ggtcgagcgc gcgcgcggcc tgttggcgga ggtgggactg gccgggctgg agcagcgcaa
     7741 gcccgaccag ctctcgggcg ggcagcagca gcgggtggcg gtggcgcgcg cgctggtgtc
     7801 gcgaccggcc ctgctgctgg ccgatgaacc caccggcaac ctcgacagca cgtccgccgc
     7861 ggaagtcttc cagttgttcc gcagcttcaa ccgcgagttc ggctgcgccg tcctgctggt
     7921 cacccacgat ccgcgcctgt ccgcgcaatg cgaccgcacg gtcaccctgg tcgacgggcg
     7981 catcgccagc gatacccgcg cgtcatcgcc tgcgggctga gcacgcggct gcggcgaagg
     8041 tcgaatggcc ccgcgccgcg ccgcgctggc agaatcgcca gggcgatgcc gcgcgggagg
     8101 atgaccatgg acgccgtgca cgggctcgac ctcggccagc cgccgttcga cctgctcgac
     8161 gatgccggcc gcgcgcgact ggctgcgcgg gtggacatgg gctaccaccc gcgcgggcag
     8221 gtgatcatcg ccgccggtgc gccgtcggaa cacgtggtgg tgctgatgaa gggccaggtc
     8281 cacgccttcg acctggacgg agccggccac gagcagcgct tcgccgatta cggccccggc
     8341 gacatcttcg gcgcgtgggc agtgatggcc ggacgcgccc gccacagcta ccgcgcggac
     8401 accgatgtgg tcagcttcct gattccggcg ggcctgttcc gcacgttgtt gtcggagcaa
     8461 cccgcgttcg ccgcgtggtt ccaggaagga ctcgcggtga agggccagct tgccgatcgc
     8521 gcacgcggtt ccgaactggc cgaactgatg gtgacccagg tcggtgccgc acagctggcg
     8581 ccggcggtcc gcgtaccggc gacgatgagc atcgccgatg ccagcgagcg cctgcgcgag
     8641 gagcgcgtgg actgcctgct ggtggacgat cccgcgcacc cgcagcccgg cgtggtcacc
     8701 cgcaccgacc tgctggaggc actgacccgc gcgcgccagc cgctggaagc cctggtgggg
     8761 ccgctggcca gccgcccact ggtcgcgcag cagaccaccg acgtcctgtt ccaggcgctg
     8821 gtgacgatgg ccgagcgcca catcgagcgc atcgccgtgc gcgacggcga ggcgatcgtc
     8881 ggcaccctcg gcatggccga ggtgctctcg cattacgcca gccattcgca cctgatcagc
     8941 ctgcgcctcg cgcgtgcgcg cagcctggac gaagtggccg acaccgcgcg cggcatgacc
     9001 cggctggtcc gcagcctgca cgcgcagggc gcgcggatgc cggcgctgat ggagctggtc
     9061 agcgcactca acagccgggt gatggcgcgg ctgttcgaat tgctggtacc gcgcgaactg
     9121 cagggccgca tgtgcctgct ggtgctgggc agcgaaggcc gccgcgagca gttgctgaag
     9181 accgaccagg acaacgcgct ggtggtcgac gacgacctcg actggcccga gctggacgcg
     9241 accatggcgc gcttcaccga ggcgatgggc gaggtcggct atccgccctg cccgggcggg
     9301 gtgatggtgt ccaacccgca ttggcggatg agcgcgacgc aatggcgggc gcgactggac
     9361 cagtggcgct cgcactacgc cgcgcaggcg gcgatggacc tggcgatcac cctcgacgcg
     9421 cggccagtgg ctggcaacgc ggcgctgttc gcaccgctcg atgcgcgcct gcaagcgctc
     9481 ggtggcgacg agcgcctgct gcacgcgatg gccgcggcgg tgctgcagtt cgaaagcccg
     9541 atctcgatgt tcggccagct gcgccaggac gaacgcggcg tggacctcaa gcgcagcggc
     9601 atcttcccga tcgtgcacgg cctgcgctgc ctcgcgctga agcatggcat cgcccagcgc
     9661 aacagcttcg cccgctgcga ggcgctggtg ccggccggcg ggctggatgc cacgttggcc
     9721 cgcgacctcg cgcagacgct ggcggtgttg cagcgcctgc ggctcgacgc gcaactggcc
     9781 acgctcgatg ccggcggcgt gcccgacaac ctggtgcaac ccgcggcgct gcgccggctg
     9841 gaccgcgaat tgctgcgcga cgcgctgcgc gtggtcaagg cgttccagca gcacgtgcgc
     9901 gtcgtgttcc acctgggcga ctgacatgca cccgctgtcg cgctggtgga tgcggcgccg
     9961 ctacggcgcg ggcgaatggg gcggcctgtt cgcaccggcc cccgatggcg aatgggtcag
    10021 cctggacctg gaaaccaccg gcctggaccc ggcgcgcgac cacatcctca gcctcgccgc
    10081 ggtgccggtg cgcgatggcc gcgtcggcct gtccgaacgt ttcgaacgcc gcgtgcacac
    10141 cgcgcgcgcg ttcggcatcg aatcgatccg ccatcaccgc atcacgccgg acgaagccag
    10201 caccggcgag caggtcaccg atgtcgtgcg tgaattcctg cactggctcg ggccgcgccg
    10261 cctgctcggc taccacctcg gcttcgacct cgacatgctc cgcccgcacg tgcgcgcgct
    10321 ggcaggcttc gagctgccca accccaccac cgatcttgcc gacgaagtcg ctgcgcgcca
    10381 gcgtgcgcaa cgcccggacg cgccggtcga cctcgacctg gtgcacatcg ccacgcgcct
    10441 cggcgtgccg atgatcgggc ggcataccgc gctgggcgat gcaacgacgg tggggttgtg
    10501 ctgg
//
LOCUS       DAOOCX010000082        10485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_5978_length_10485_cov_9.048802, whole genome shotgun sequence.
ACCESSION   DAOOCX010000082 DAOOCX010000000
VERSION     DAOOCX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5978_length_10485_cov_9.048802"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PK399_08515"
     CDS             complement(<1..460)
                     /locus_tag="PK399_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPW13200.1"
                     /translation="MAELAKEIIPVNLEDEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELGAHSNKPYYKSARIVGDVIGKYHPHGDQSVYDTLVRMAQPFSLRYLL
                     VDGQGNFGSVDGDSAAAMRYTEARMARLTHELMADIDKETVDFQPNYDEKE"
     gene            complement(824..1882)
                     /gene="mtnA"
                     /locus_tag="PK399_08520"
     CDS             complement(824..1882)
                     /gene="mtnA"
                     /locus_tag="PK399_08520"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPW13201.1"
                     /translation="MDNILDFDRYDRIRPIRWTGESLELLDQRNLPFAVAYDTSTTSD
                     DVAAAIHALTVRGAPAIGIAAAWGVVLAAREVQAATGREAALALAPAMARLNASRPTA
                     VNLAWALARMQRALETAGSDWRAVLEREARAIETEDLAANRHMGALGAALIAPGSGVL
                     THCNTGSLATAGFGTALGVIRAGVAAGRIDKVFAGETRPWNQGARLTVWELQQDGIDA
                     TLIADAAASHLMKTGKVQWVIVGADRICANGDTANKIGTYQLAIAARHHGVKFMVVAP
                     SSTVDMATASGELIEIEERDTAELLSFKGARTVSEGVRAWNPVFDVTPHDLIDAIVTQ
                     LGVVERPDAARMQALFKP"
     gene            complement(1955..2731)
                     /locus_tag="PK399_08525"
     CDS             complement(1955..2731)
                     /locus_tag="PK399_08525"
                     /inference="COORDINATES: protein motif:HMM:NF022664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HPW13202.1"
                     /translation="MRRLIFATAMFAAASAAPAIAAPQSWQEYGPGTGVVRCESNDGR
                     MNRCPLPGGGRVVLERQHSRTACIEGRSWGQDRGGVWVDDGCRADFRVAGYGGGYNDG
                     YSGGGYGNGGYNDRYGQTFRCESDDGRTRQCNGNGRGRAQLVKQLSRAACIEGRTWGS
                     NYNGVWVSQGCRGEFAMGGGYGSGPYGGGYGGPNNGFGRLFRCESNDGRTHECAANTR
                     AGVQLVRQLSRAACIQGRTWGYGRNGIWVSDGCRAEFRSY"
     gene            complement(2844..3809)
                     /gene="epmA"
                     /locus_tag="PK399_08530"
     CDS             complement(2844..3809)
                     /gene="epmA"
                     /locus_tag="PK399_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HPW13203.1"
                     /translation="MPSASRDALRLRAWVNRLVREFFHARNVLEVETPAMSLAGNTDP
                     NIASFSLEFSGRTDGGPRARWLRTSPEFALKRLLAAGVGDCYELGRVFRDGEAGGRHN
                     PEFTMLEWYRVGWNHLRLIDETAELVRAALSLLGRDAALRVVRYRDLYHDALGIDPFT
                     ASDGELRAALGDLEIDPAGLTRDDWLDLLMTHRLQPAFDPAGLLAIVDYPASQCALAR
                     LRDDGNGHPVAERFELYLGAIELANGYHELLDAGEQRARFQRDGIVREARGDVVPPTD
                     ERLLIALDAGLPSCAGVALGVDRLLMAMLQTPRIADVLAFDFARG"
     gene            complement(3863..6211)
                     /gene="ligA"
                     /locus_tag="PK399_08535"
     CDS             complement(3863..6211)
                     /gene="ligA"
                     /locus_tag="PK399_08535"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPW13204.1"
                     /translation="MPAMTDTATRIAELRRRIEDANRRYHGLDAPDITDAQYDALVRE
                     LDALERAHPELASTDSPTRKVGSAPSGRFAEVVHAVPMLSLGNAFSDDEVGDFVRRIR
                     ERLDRDALSFSAEPKLDGLAISLRYENGLFVQGATRGDGATGEDVTANLRTIRDIPRE
                     LAGAGWPKALEVRGEVYMGRADFERWNEQARLHGGKVLANPRNGAAGSLRQIDSALTA
                     KRPLSFFAYGIGQVEGGTLPATHSATLAKLREWGFPVSELATVVQGVEGLLGYYRRIG
                     EARDSLPFDIDGVVYKVDDYAAQRELGFVSRAPRWAIAHKFPAQEQATVLESIEVNVG
                     RTGAVTPWALMQPVQVGGVTVTRATLHNADHVARLDVRNGDTVIVRRAGDVIPEVVQV
                     VLEQRPANTVPWSMPMACPVCGSEVVREEGAAVWRCSGELSCPAQLVQAVFHFASRRA
                     MDIDGLGERYIESLADFGYLQDVSDLYRLTLDDLLEMKRRAEERDGAVPETVKAGKVA
                     TKWADNLIAAIDRSRDTTLARFLYALGIEHVGESTAKALAQWFGDLALIRHLPWPLFK
                     RVPDIGGEVARAIGHFLDQAGNQQVIDRLLERGVRIGDAHAPNPKLGDGLDLAALLAD
                     LEIPRVTPVRAAQLASAFADAEALVDAPAHAMVTAGLPTDSANALAAWLEDEANAGLL
                     LRSAQAMNALRDRLPERSDDVAGPLEGKTVVLTGTLAAMGRDEAKEKLEALGAKTAGS
                     VSRKTSFVVAGTDAGSKLAKAEELGIDVWDEAALLAFLAQYA"
     gene            complement(6273..6986)
                     /gene="zipA"
                     /locus_tag="PK399_08540"
     CDS             complement(6273..6986)
                     /gene="zipA"
                     /locus_tag="PK399_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HPW13205.1"
                     /translation="MSDMTLLRIGIAVAMSLLFGAIIYFGRGRKNAQGRRVPRDGASG
                     EARTEPSLGDQFAEGWDQGPARQEELALDGSPAQSELGKRVSEQFDRIVSLYVAAKAG
                     NKLRGQDIVVAAEKAGLTFGHMDVFHRLVDGKPELGPIFSVANIIKPGSFEMARIAEI
                     ETPAIAFFLTLPAPVAALDAWETMEPAAQRMADLLDGVVLDEERNALGRQRVQHIRDE
                     LRAYDRQHAAPPLSKSNRW"
     gene            complement(7000..>10485)
                     /gene="smc"
                     /locus_tag="PK399_08545"
     CDS             complement(7000..>10485)
                     /gene="smc"
                     /locus_tag="PK399_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPW13206.1"
                     /translation="SGFKSFVDPTTLHLPTNMTGVVGPNGCGKSNIIDAIRWVMGESA
                     ASRLRGDSLTDVIFSGSSARKPVSQAMVELVFDNSDHTITGEYAAFNEISVKRMVSRD
                     GQSSYYLNGAKCRRRDITDLFLGTGLGPRSYSIIEQGMISQVIDAKPEELRVYLEEAA
                     GISKYKERRKETETRIRHTRENLDRLNDLREEVGKQLQHLARQAKQAEQYTAIQAERR
                     IKDVEWKALEFRALDGKLQRLREKLAQEETRLEQLIAEQREAERELETGRERRETASD
                     ALNAAQAAVYRVGADLARVEQQIAHQRELSTRLQRARDEALATLSELGAHISDDQQKL
                     DALREAVADAEPRLELLQEGDLARQDALRDAELQLADWQQRWDAHGRQQAEAARAGDV
                     ERTRIEHLDRQVLDADRRREQLTSERAGLDLDTLADAFAELQLQHETQKESLDTLGEQ
                     VELRKQATADAQDQQRARQAELAELRKQAQAARGRLSSLETLQSAALGQEQGAATQWL
                     KAQGLDDAARVGERLQVEAGWENAVEGALGQLIEAVLVDAPEALVDALGELGDGRLAL
                     VASADDVDGEAYAPTSLAAKVQGPRAIRRLLSRLHGADTLADARALQAQLGDGESVIT
                     RNGERLGAGWVRVVRSGAAKQGALLREKEIQSLRGEIDGLVDRERELEALLTRLRDQL
                     LAAEQQREDAQRALYLAHRGVSELAGQLQSQQGKLESARNRIERIDAELAQLASTLEA
                     AQAQSREARQRVEHAVSAMGDLETARQALDAERRQLAEARDAARLAARESRDAAHALA
                     LTLESQRVQVVALAQALERMGGQRGQLDSRLGDLAAQLAEGDAPVQSLENERQVALTE
                     RVRSERELAEARSTLEGIDNDLRGFEQVRQQRDAQALAQREAIGQRRLDQQALAIKAD
                     QFAAAVVEAGFAMDDVINTLTEDADASLWEKAVADFDAKLRRLEPVNLAAIAEHAEAA
                     QRKEYLDAQDADLTTALETLEEAIRKIDRETRGRFKDTFDRVNSGVQELYPRLFGGGH
                     AYLELTGDDLLDTGVAIMARPPGKRVSNISLLSGGEKAMTAVALVFAIFRLNPAPFCL
                     LDEVDAPLDEANVGRFTAMVSEMSEQVQFLFVTHNKATMEAAQQLSGVTMREPGVSRL
                     VSVDLAEAARLAGAA"
BASE COUNT         1550 a         3626 c         3543 g         1766 t
ORIGIN      
        1 gttccttctc gtcgtagttc ggctggaaat cgacggtttc cttgtcgatg tcggccatca
       61 gctcgtgggt gagccgcgcc atgcgcgcct cggtgtaacg catcgccgcg gcggagtcgc
      121 catcgaccga accgaagttg ccctgcccgt ccaccagcag gtagcgcagc gagaacggct
      181 gcgccatgcg caccagggtg tcgtacaccg actggtcgcc gtgcgggtga tatttaccga
      241 tgacgtcgcc gacgatgcgg gcggacttgt agtacggctt gttgctgtgc gcgccgagct
      301 cgtgcatggc gaacagcacg cgccggtgca ccggcttgag gccatcgcgg acatccggga
      361 gggcgcgccc cacgatcacg ctcatggcgt aatcgaggta gctgcgacgc atctcgtctt
      421 ccaggttgac ggggatgatt tccttggcga gctcggccat cgtgcgtccg gttccgtaca
      481 gaaggtgcag caactttcgt gacccgcggc gaccgagcgg aggcgactcc gcgcaggact
      541 tccggggggc ttttcagcaa cgtgaaatca tagcattttc gggacgcttc cgtaccgtcg
      601 attccgacga agaatcaatg ggttacggga ctttttggca cccaacgcca catcgcccaa
      661 ccgccttttc atccagcagc gaaattctgc agtgagtggg tgtggcgtgg cgccgcagag
      721 cgggccatgg atggcccgcg gcgacgcgtc gaacaggacg ttcgcacgcg tcgaggaggc
      781 gccatgccgc gcccactcat ccccggaaaa tgaacgcacg agatcagggc ttgaacagcg
      841 cctgcatccg cgccgcatct gggcgttcga ccacacccag ctgggtcacg atcgcatcga
      901 tcagatcgtg cggagtaaca tcgaacaccg ggttccaggc gcgcacccct tcggagacag
      961 ttcgcgcgcc cttgaaggac aggagctccg cggtatcgcg ctcctcgatc tcgatcagct
     1021 cgcctgaggc ggtcgccatg tccacggtcg aggacggtgc gaccaccatg aacttgaccc
     1081 cgtggtggcg cgcggcgatg gccaactgat aggtgccgat cttgttggcg gtatcgccgt
     1141 tggcgcagat gcggtccgcg ccgacgatga cccactgcac cttcccggtc ttcatcaggt
     1201 gcgaggccgc ggcgtcagcg atcagggtgg cgtcgatgcc gtcctgctgc agttcccaca
     1261 cggtcagccg cgcgccctgg ttccacgggc gggtctcgcc agcgaacacc ttgtcgatgc
     1321 ggcccgccgc cacgcccgcg cggatcaccc cgagcgcggt gccgaagccg gcagtggcca
     1381 gcgagccggt attgcagtgg gtcagcacgc cgctgcccgg cgcgatcagc gcggcgccca
     1441 atgcgcccat gtggcgattg gcggccaggt cttcggtctc gatggcgcgg gcttcgcgct
     1501 ccagcaccgc gcgccaatcg ctgccggcgg tttccagcgc ccgctgcatg cgcgccagtg
     1561 cccaggcaag gttaaccgcc gtgggacgcg aggcattgag gcgggccatc gcgggtgcga
     1621 gcgcgagcgc ggcctcgcgg ccagtggcgg cctgcacctc gcgcgccgcc agtaccacgc
     1681 cccaggcggc ggcgatgccg atcgccggtg cgccgcgcac ggtcagtgcg tggatcgccg
     1741 cggctacgtc gtcgctggtc gtgctggtgt cgtaagccac cgcgaacggc aggttgcgct
     1801 ggtccagcag ttccagggat tcaccggtcc agcggatcgg gcggattcgg tcgtaacggt
     1861 cgaaatcgag gatgttgtcc atgctcggat tatccgccat ccggaaacgg aaccggcgcc
     1921 acgtgggcgc cggtttcgga catcaagcga gccatcaata gctgcggaat tcggcgcggc
     1981 aaccgtcgga cacccagatg ccgttgcggc cgtagcccca ggtacgcccc tggatgcagg
     2041 cggcgcgcga cagctggcgc acgagttgca cgccggcgcg ggtattggct gcgcattcat
     2101 gcgtgcgacc gtcgttggat tcgcagcgga acagacgccc gaaaccgttg ttggggccac
     2161 cgtagccacc gccatacggg ccgctgccgt acccgccgcc catcgcgaat tcgccgcggc
     2221 acccctgcga tacccagacg ccgttgtagt tcgaacccca ggtgcgtccc tcgatgcagg
     2281 ccgcgcgcga caactgcttc acgagttgcg cgcggccgcg accgttgccg ttgcactggc
     2341 gcgtgcgccc atcgtccgac tcgcagcgga aggtctggcc gtatcgatcg ttgtaaccgc
     2401 cattgccgta accgccaccg gagtaaccat cgttgtaacc accgccgtaa ccggcaacgc
     2461 ggaagtccgc gcggcagcca tcatccaccc acaccccacc gcgatcctgt ccccaggacc
     2521 gcccttcgat gcaggcggtg cgcgaatgct ggcgttccag caccacgcgg ccgccgccag
     2581 gcagcgggca gcggttcatg cggccgtcgt tggattcgca acgcacaact cccgtccccg
     2641 ggccgtactc ctgccaggac tgcggtgcgg cgatggcggg tgcggcggac gccgcggcga
     2701 acatggcggt agcgaagatc aggcgacgca tggtgacccc tccggtcgtt gtcctggggc
     2761 gtactgttag gcatgccgga ttaacaaaaa gcgaaggcaa tccgaacgcc tgccgaacgc
     2821 acgcttcccg ttcagtcgga tcgtcagccg cgggcgaaat cgaacgccag cacgtcggcg
     2881 atgcgtggcg tctgcagcat cgccatcaac agtcgatcca cgcccagggc gactccggcg
     2941 cagctgggca ggcccgcatc caacgcgatc agcaggcgtt cgtccgtcgg cgggacgaca
     3001 tcgccccgcg cctctcgcac gatgccgtcg cgctggaatc gcgcgcgctg ttcgccggca
     3061 tcgaggagtt cgtggtagcc gttcgccagt tcgatggcgc cgagatagag ctcgaagcgt
     3121 tcggccaccg ggtggccatt gccgtcgtcg cgcaagcgcg ccagcgcgca ttgcgatgcc
     3181 gggtagtcga cgatcgccag caagccggcg ggatcgaacg cgggctgcag ccgatgggtc
     3241 atcagcaggt cgagccaatc gtcgcgggtc aaaccggcgg ggtcgatctc gaggtcgcca
     3301 agcgcggcgc gcagttcccc atcgctcgcc gtgaacggat cgatgccgag tgcgtcatgg
     3361 taaagatcgc gataacggac gacgcgcagt gccgcgtcgc gccccagcaa cgacagcgcc
     3421 gcacgcacga gctccgcggt ctcgtcgatc agccgcaggt ggttccagcc gacccgatac
     3481 cactcgagca tggtgaactc ggggttgtgg cgaccaccgg cttcaccatc gcggaacacc
     3541 cggccgagtt cgtagcagtc gccgacgccg gccgcgagca ggcgcttcaa ggcgaattcg
     3601 ggcgacgtgc gcagccagcg ggcgcgcggc ccaccatcgg tgcggccgga gaattccagc
     3661 gagaacgagg cgatgttggg atcggtgttg ccggccagcg acatcgccgg ggtttccact
     3721 tccagcacgt tgcgcgcatg gaaaaactcg cgcaccagcc ggttcaccca cgcacgcagg
     3781 cgaagtgcgt cgcgggacgc ggatggcatc cacgcggcat cgggatcgag gccgtcgcgc
     3841 cagccgggac ccggcgtgaa cgtcatgcgt attgcgccag gaacgcgagc aacgccgcct
     3901 cgtcccagac gtcgatgccg agttcctccg ccttggccag cttcgatccg gcatcggtcc
     3961 cggcgacgac gaaactggtc ttcctcgaca cactgccggc ggtcttcgcg cccagcgctt
     4021 ccagtttttc cttggcttcg tcacgaccca tcgcagccag cgtgccggtc agcaccacgg
     4081 tcttgccctc gagcgggccg gcgacgtcgt cgctgcgctc cggcaagcgg tcgcgcaacg
     4141 cgttcattgc ctgggcgctg cgcagcagca gtccggcatt ggcctcgtct tccagccagg
     4201 cggcaagcgc gttggcgctg tcggtcggca ggccggccgt gaccatcgcg tgcgcaggcg
     4261 catcgaccag cgcctccgcg tcggcaaacg cgcttgcgag ttgcgccgca cgcaccggcg
     4321 tgaccctggg gatttcgaga tcggccagca gcgcggccag gtccagtccg tctcccagtt
     4381 tcgggttggg cgcatgcgcg tcgccgatgc gcacgccgcg ttcgagcagg cgatcgatca
     4441 cctgctggtt gccggcctgg tcgaggaaat ggccgatggc gcgcgcgact tcgccgccga
     4501 tgtcggggac gcgcttgaac aacggccacg gcaggtggcg gatcaacgcg aggtcgccga
     4561 accactgcgc cagggccttc gccgtgctct cgccgacatg ttcgatgccg agcgcgtaga
     4621 gaaaccgtgc gagcgtggtg tcgcggctgc ggtcgatcgc ggcgatcagg ttgtcggccc
     4681 atttcgtggc gacctttccg gctttcacgg tttccgggac tgcgccgtca cgctcctcgg
     4741 cgcgacgctt catttcgagc aggtcgtcca atgtcagccg atacaggtcg gaaacatcct
     4801 ggaggtaacc gaagtccgcc agcgactcga tgtagcgctc gccaaggccg tcgatgtcca
     4861 tcgcccgccg cgacgcgaag tggaataccg cctgcaccaa ctgcgccggg cagctgagtt
     4921 cgcccgagca acgccagacc gcggctcctt cctcgcgcac cacttcggaa ccgcagaccg
     4981 ggcacgccat cggcatcgac cacggcaccg tgttcgccgg tcgctgttcc agcacgacct
     5041 gcaccacttc cgggatcacg tcgccggcgc ggcgcacgat gacggtgtcg ccgttgcgca
     5101 cgtccaggcg cgcgacatgg tcggcgttgt gcagggtggc gcgggtgacg gtgacgccac
     5161 cgacctgcac cggctgcatg agcgcccacg gcgtgaccgc gccggtgcgg ccgacgttga
     5221 cctcgatcga ttccagcacc gtggcctgtt cctgcgccgg gaacttgtgc gcaatggccc
     5281 agcgcggcgc gcgagagacg aagcccagtt cgcgctgcgc agcgtagtcg tcgaccttgt
     5341 agaccacgcc gtcgatgtcg aacggcaggc tgtcgcgggc ctcaccgatg cggcggtagt
     5401 agcccagcaa tccctcgacg ccctgcacca ccgtggccag ttcgctgacc gggaaacccc
     5461 attcgcgcag cttcgccagc gtggccgaat gggtggccgg cagggttccg ccttcgacct
     5521 ggccgatgcc ataggcgaag aagctcaacg ggcgcttcgc ggtgagtgcc gagtcgatct
     5581 gccgcagcga gccggcggcg ccgttgcgcg gattggcgag caccttgccg ccgtgcaggc
     5641 gcgcctgttc attccagcgt tcgaagtcgg cgcggcccat gtagacctcg ccacggactt
     5701 ccagggcctt cggccagcct gcacccgcaa gttcacgcgg aatatcgcgg atggtgcgca
     5761 ggttggcggt cacgtcctcg ccggtggcgc catcgccgcg ggtcgcgccc tgcacgaaca
     5821 ggccgttttc gtagcgcagg ctgatcgcca ggccgtcgag tttcggttct gcggaaaacg
     5881 acagcgcatc gcggtcgagc cgctcgcgga tccggcgcac gaagtcgccc acttcgtcgt
     5941 cgctgaaggc attgcccagc gacagcatcg gcaccgcgtg caccacttcg gcgaagcggc
     6001 cggacggtgc ggaaccgact ttgcgcgtcg gcgaatcggt gctggcgagc tccggatgcg
     6061 cgcgctccag cgcatcgagt tcgcgcacca gcgcgtcgta ctgcgcatcg gtgatgtccg
     6121 gcgcgtcgag cccgtggtag cgccggttgg cgtcctcgat gcggcggcgg agttcggcga
     6181 tgcgggtggc ggtatcggtc atcgcaggta ttgtcggctg atcgcaccct caccccaacc
     6241 ctctcccgcc cgcgggagag gggatatggg catcaccagc gattcgactt gctcagcggc
     6301 ggcgccgcgt gctggcgatc ataggcgcgg agttcgtcgc ggatgtgctg gacgcgctgg
     6361 cggcccagtg cgttgcgctc ttcgtcgagc accacgccgt cgagcaggtc tgccatgcgc
     6421 tgcgcggcag gctccatggt ttcccacgca tccagtgcgg cgaccggcgc cggcagggtc
     6481 aggaagaacg cgatggccgg ggtttcgatc tcggcgatgc gcgccatctc gaaactgccg
     6541 ggtttgatga tgttggcgac gctgaagatc gggccgagtt ccggcttgcc atccacgagg
     6601 cgatggaaca cgtccatgtg cccgaacgtc aggccggcct tctcggcggc gaccacgatg
     6661 tcctgcccgc gcagcttgtt gccggccttg gccgccacgt acagcgagac gatgcggtcg
     6721 aactgctcgg acacccgctt gccgagttcg ctctgcgcgg ggctgccgtc cagggccaac
     6781 tcttcctgcc gtgccggacc ctggtcccag ccctcggcga actggtcgcc cagcgagggt
     6841 tcggtgcgcg cttcgccact cgcgccatcg cgcgggacgc gccggccctg ggcgttcttg
     6901 cggccccggc cgaagtagat gatcgcgccg aacagcagcg acatcgccac ggcgataccg
     6961 atgcgcagca gggtcatgtc ggacatcagg acagcctcct caggcggcgc cggccaggcg
     7021 cgcggcttcg gcgaggtcca cggacaccag gcgcgacacg cccggctcgc gcatggtcac
     7081 gccggacagt tgttgcgcgg cttccatggt cgccttgttg tgggtcacga acaggaactg
     7141 tacctgctcg ctcatttcgc tgaccatcgc ggtgaagcga ccgacattgg cttcgtccag
     7201 cggcgcgtcc acctcgtcca gcaggcagaa cggtgccgga ttgagccgga agatcgcgaa
     7261 caccagggcg accgcggtca tcgccttctc gccacccgac agcagcgaga tgttggacac
     7321 gcgcttgccg ggggggcgcg ccatgatcgc cacgccggtg tccagcaggt cgtcgccggt
     7381 cagttccagg taggcgtggc cgccgccgaa caggcgcgga tacagctcct gcacgccgga
     7441 attgacccgg tcgaaggtgt ccttgaaccg cccgcgggtc tcgcggtcga tcttgcggat
     7501 cgcttcttcc agcgtctcca gcgcggtggt gaggtcggcg tcctgcgcgt ccaggtactc
     7561 cttgcgctgc gcggcctcgg catgttcggc gatggcggcc aggttgaccg gctcgagcct
     7621 gcgcagcttg gcgtcgaagt cggcgacggc cttttcccag agcgacgcat ccgcgtcctc
     7681 ggtgagcgtg ttgatgacgt cgtccatcgc gaagccggcc tcgaccacgg cggccgcgaa
     7741 ctggtccgcc ttgatcgcca gcgcctgctg gtcgagccgg cgttggccaa tcgcctcgcg
     7801 ctgcgccagc gcctgcgcat cgcgctgctg gcgaacctgt tcgaagccgc gcaggtcgtt
     7861 gtcgatgcct tcgagggtcg aacgcgcctc ggccagttcg cgctcgctgc gcacgcgctc
     7921 ggtcaatgcg acctggcgtt cgttctccag cgactgcacc ggcgcatcgc cctcggcgag
     7981 ctgcgcggcc aggtcgccca agcgcgaatc cagctgtcca cgctggccgc ccatgcgctc
     8041 cagcgcctgc gccaaggcga ccacctgcac ccgctgcgat tccaaagtga gcgcgagcgc
     8101 gtgcgcggcg tcgcgggatt cgcgtgcggc caggcgtgcg gcatcacgtg cctccgccaa
     8161 ctggcggcgc tcggcgtcga gagcctgccg ggcggtttcc agatccccca tcgccgacac
     8221 cgcatgctcg acccgctggc gcgcctcgcg cgattgcgcc tgcgcggctt ccagcgtgct
     8281 ggccaactgc gccagttccg cgtcgatgcg ttcgatgcgg ttgcgtgcgg actccagctt
     8341 gccctgctgg ctctgcaact gtccggctag ctcggaaaca ccacggtgcg cgagatacag
     8401 ggcgcgctgg gcgtcctcgc gctgctgttc ggcggccagc aactggtcgc gcaggcgcgt
     8461 cagcagcgct tccagttcgc gctcgcgatc gaccaagccg tcgatctcgc cgcgcagcga
     8521 ctggatctcc ttctcgcgca gcagcgcgcc ctgcttggcc gcgcccgaac gcaccacgcg
     8581 gacccagccg gcgcccaggc gttcgccatt gcgggtgatg accgactcgc catcgcccaa
     8641 ctgcgcctgc agggcgcgcg catcggccag ggtgtccgca ccatgcaggc gcgacagcag
     8701 gcgacggatc gcgcgcggcc cctgcacctt cgccgcgagc gaggtcggcg cataggcttc
     8761 gccatcgacg tcgtcagccg atgcgaccag cgccaggcga ccatcgccga gttcaccgag
     8821 cgcatcgacc aaggcttccg gcgcatcgac cagcaccgct tcgatcaact ggcccagcgc
     8881 accttcgacc gcgttttccc agccagcctc gacctgcagg cgctcgccga cgcgggcggc
     8941 atcgtccagc ccctgcgcct tcagccattg cgtcgccgcg ccctgctcct ggcccagcgc
     9001 tgccgactgc agggtctcaa gggatgacaa gcgcccgcgc gcggcctgcg cctgcttgcg
     9061 cagttcggcc agttcggcct ggcgtgcacg ctgctggtcc tgggcgtcgg cggtggcctg
     9121 cttgcgcaac tcgacctgct cgcccagcgt gtccagcgat tctttctgcg tctcgtgctg
     9181 caactgcagc tcggcgaagg catcggccag ggtgtcgagg tccagcccgg cgcgttcgct
     9241 ggtcagctgt tcgcggcgac ggtcggcatc cagcacctgt cgatcgaggt gctcgatgcg
     9301 ggtgcgttcc acgtcgccgg cacgcgcggc ctcggcctgc tggcggccat gcgcgtccca
     9361 gcgctgctgc cagtcggcca gttgcagctc ggcatcgcgc agcgcgtcct ggcgcgcgag
     9421 gtcgccttcc tgcagcagtt ccagtcgcgg ctcggcatca gccaccgctt cacgcaaggc
     9481 atccagcttc tgctggtcgt cggagatgtg cgcgccgagt tcggacaggg tcgccagcgc
     9541 ttcgtcgcgg gcacgctgca ggcgggtcga cagttcgcgc tggtgggcga tctgctgttc
     9601 gacccgggcc aggtcggcgc cgacgcgata gaccgcggcc tgcgccgcgt tcagggcgtc
     9661 gctggcggtc tcgcggcgct cgcggccggt ttccagttcg cgctcggcct cgcgctgctc
     9721 ggcgatcaac tgttcgaggc gggtttcttc ctgcgccagc ttctcgcgca ggcgctgcag
     9781 cttgccgtcc agggcgcgga attccagcgc cttccattcc acgtccttga tccggcgctc
     9841 ggcctggatc gcggtgtact gctcggcctg cttggcctgc cgcgcgaggt gctgcagctg
     9901 cttgccgact tcctcgcgca ggtcgttgag gcggtccagg ttctcgcggg tgtggcggat
     9961 ccgggtttcg gtttccttgc ggcgttcctt gtacttggag atgccggcgg cctcttccag
    10021 gtagacgcgc agttcctcgg gcttggcgtc gatgacctgc gagatcatcc cctgctcgat
    10081 gatcgaatag ctgcgcgggc ccaggccggt gccgaggaac aggtcggtga tgtcgcggcg
    10141 gcggcacttg gcgccgttga ggtagtagct gctctggcca tcgcggctga ccatgcgctt
    10201 gaccgagatc tcgttgaacg cggcgtactc gccggtgatg gtgtggtcgc tgttgtcgaa
    10261 caccagctcg accatcgcct ggctgacggg cttgcgcgcg gagctgccgg agaagatcac
    10321 gtcggtcagc gaatcgccgc gcaggcggct ggcggcggac tcgcccatca cccagcggat
    10381 ggcgtcgatg atgttggact tgccgcaacc gttcggcccg accacgccgg tcatgttggt
    10441 cggcaggtgc agggtggtcg gatcgacgaa ggatttgaag ccgga
//
LOCUS       DAOOCX010000083        10442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_6011_length_10442_cov_7.208819, whole genome shotgun sequence.
ACCESSION   DAOOCX010000083 DAOOCX010000000
VERSION     DAOOCX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10442
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6011_length_10442_cov_7.208819"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..668)
                     /locus_tag="PK399_08550"
     CDS             complement(294..668)
                     /locus_tag="PK399_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13207.1"
                     /translation="MIVLEQGPAGNSGFSRAPAVIAIPESGPDAEYPNFVHGACPRPA
                     SWEMERPEELVPVLQTAWDEPESSWSAKRLGVKVHVTIQAEPRSCLGFDELLGSSLRS
                     LAIILVQRLTLVLDDLVRDRLD"
     gene            1291..1671
                     /locus_tag="PK399_08555"
     CDS             1291..1671
                     /locus_tag="PK399_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW13208.1"
                     /translation="MQRATGIGGIFFKSRDPKALGAWYRDHLGLDVTDWGGVVFQWGG
                     PGSPPGMTLWSPFAQDTDYMAPSTSSFMINFRVDDLDALLEALRQEGCNVVDKTDSSD
                     QGKFGWVIDPEGNKVELWQPPAGQ"
     gene            1730..2128
                     /locus_tag="PK399_08560"
     CDS             1730..2128
                     /locus_tag="PK399_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW13209.1"
                     /translation="MNSHPIETWHRLVATNDPSGLAGLLADDAVFLSPIVHTPQHGKA
                     LVTAYLSAAFQVFFNPTFHYVRQILGPSDAMLEFEAEIDGVLVNGVDIVKWNGAGQII
                     EFKVMIRPLKAVNLIHERMGAILAAARRTG"
     gene            complement(2176..3294)
                     /locus_tag="PK399_08565"
     CDS             complement(2176..3294)
                     /locus_tag="PK399_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPW13210.1"
                     /translation="MDFSFTDEQQLLQDSIRRYLESSYTIEHRAGILASESGWSATTW
                     TELADLGLLALDIDEADGGIGAGPVGMMLAAQAIGAGLLVEPFHSSAVLATRMVSALA
                     RDTQRERLLGALASGEAIAVFAHDEGGDWFAPCSTIATRSEDGWRLQGRKTCVYHATM
                     ARWLLVSARVDEGVGVFVVDRDTAGVDLRDYRTVDGMRAADIELDLMVAADSRLGDVD
                     AREAIDEALDRGIAAWCADALGALDRVLAATVQYARDRVQFGTAIGRLQALQHRMADM
                     YMQVEQARSMVYLATSACLQSDAHERREAVSGAKVLVGQAARKVGQEAVQLHGGMGMT
                     DELGVSHCFKRLLAFELRMGSTEEHLAHYRKLAQSRAA"
     gene            complement(3307..4470)
                     /locus_tag="PK399_08570"
     CDS             complement(3307..4470)
                     /locus_tag="PK399_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPW13211.1"
                     /translation="MDLNFSADEVLFRDDVRAFLAANLPDDIRDRVRRGDESVLADDV
                     RRWQRILFERGWGAAMWPAEFGGTGWDKTRQYLFDIECFAADAPIQLPFGLKMVAPVI
                     MRYGSEVQKARFLPRILDGTDWWCQGYSEPGSGSDLASLKTRAQRDGDHYVVNGQKAW
                     NTLGQHADWIFCLVRTDPAAKPQRGISFLLIDMKTPGIRVHPTRLLDGRHEVNEIFFD
                     DVRVPVENRVGEENAGWTYAKFLLGHERTNIAGIGASRRELARLKQMAREATRNGAAL
                     LEDPIFATRVAQVELELKALEVTNLRVIFAEARQQAPGPEASMLKIRGTESLQRISEL
                     QVEALGSRALQFDGAGEDVPRVAAQYMNLRKLSIYGGSNEIQRNIIAQMVLQL"
     gene            complement(4486..5382)
                     /locus_tag="PK399_08575"
     CDS             complement(4486..5382)
                     /locus_tag="PK399_08575"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW13212.1"
                     /translation="MKIETLRGKVAVITGAGSGFGREFARIAAEEGMSLVLVDVQADA
                     LAETVAGLDGIDILQRTLDVTDGVAMEALAADAFTRFGAVHLLFNNAGVGCTGLLWEA
                     TTRDWEWVLGVNLWGTIHGVRAFVPRMLANAKADPAYRGRVINTASMAGLIDPPLMGP
                     YNVAKHAVVSLSETLHQDLSLVTDQIGCSVLCPSWVATGIAKSHRNRPHDDPTRSRAA
                     ALGASMVEKAIASAKVTADDVARLTFDAIAHDRFWVLPHPQAVEGVRERADALLSGGA
                     PADPYAAIPQVRQQLAAALRAS"
     gene            complement(5379..6161)
                     /locus_tag="PK399_08580"
     CDS             complement(5379..6161)
                     /locus_tag="PK399_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW13213.1"
                     /translation="MHGNKTLFDLDGRVALITGGSRGLGLQIAEALGDYGATIAISAR
                     KQHELDAALEHLRARGIRAEAFAADASKPDAVTQLVDSVLAAFGQVDVLVNNAGASWG
                     APAEDYPLEAWDKVFDLNVRGLFALSQQVAKRSMLPRGSGRIINVASVAGLRGNAGGL
                     LKTAAYNASKGAVVNLTRALAAEWGGRGVTVNAIAPGFFPSKMSQGVIAAVGEEALMG
                     NAPLRRLGGDEDLKGAALLFASDAGQHITGQVLAVDGGYTAV"
     gene            complement(6172..7995)
                     /locus_tag="PK399_08585"
     CDS             complement(6172..7995)
                     /locus_tag="PK399_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPW13214.1"
                     /translation="MPLIPRRELKFQLHDWLRVDALLHAPRFAAHSAETFDAVMDGCD
                     RLAAEVFAPCYQAADRDEPHFDGENVSVHPQIAEAVRAFAVSGLVSATEDESVGGMQL
                     PVVVEKAALVNLYAANIGACAYPLLTMGNARLLLAHGTPAQVDAFAMPQLRGDWTGTM
                     CLSEPQAGSSLSDITTRAEYERESPFGPQYRISGRKMWISGGEHEILGNIVHLVLAKV
                     PDADGKLPAGTRGISLFIVPRSLVDADGNVGERNDVSLAGLNHKLGYRATVNCALNFG
                     EARFKPEGRAGAIGYLVGKPGEGLAGMFHMMNDARIGVGLGAVALGYAGYLLALDYAR
                     NRPQGRLPGPAGKDPSQPQQPLVAHADVRRMLLAQKSYAEGGLGLVLYCARLVDEARI
                     AREAGDAALVSSRELLLDILTPIAKSWPSQWCLAGNDLAIQVHGGYGYTRDYKVEQLW
                     RDNRLNPIHEGTHGIQGIDLLGRKVAMFDGAAFAAHGALLDASITRALDTSSAALADA
                     LSIRLTRLRDVTASLLRVADPMARLANASAYLEAFGHITVAWIWLEQVLALGDRDDDF
                     ARGKRQAARYFMQWELPRADAWLDRLADNDDTALAMRDDWF"
     gene            complement(8005..9183)
                     /locus_tag="PK399_08590"
     CDS             complement(8005..9183)
                     /locus_tag="PK399_08590"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPW13215.1"
                     /translation="MKEAVIVSTARTGLAKSWKGALNLSHGATVGGHVVRHAVERAGI
                     DPAEVEDVVMGCATPEGATGSNIARQIAIRGGLPASVPGATVNRFCASGLQAIAQTAQ
                     RIIAGEGEVFVAGGVECISTVQATINQHMLEDTWLKRRYPAIYLPMLQTAEIVAKRYG
                     IDRERQDRYGTESQVRAAAAQAAGRFDDEIVPITVRMAVADPVTRAVSTREVTLDRDE
                     GVRADTTYDAVAKIRSAVPGGVVSAGNASQFSDGASASVLMSADAASRKGLQPLGIFR
                     GFAVAGCEADEMGIGPVFAVPKLLKNAGLSVHDIGLWELNEAFAVQVLYCMDRLGIPH
                     DRLNVNGGAIAVGHPYGVSGARLVGHALIEGKRRGVKYVVVTMCVGGGQGAAGLFEVA
                     "
     gene            complement(9192..>10442)
                     /locus_tag="PK399_08595"
     CDS             complement(9192..>10442)
                     /locus_tag="PK399_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPW13216.1"
                     /translation="IPPRAIDKVGVIGAGTMGGGISMNFLNAGIPVVLVETTQEALDR
                     GVATIRRNYEASAKKGKLTDAQVEQRMALVTPSLSYDALADADLVIEAVYEDYAVKQS
                     VFEKIDAVAKPGAILATNTSTLDVDRIAAFTKRPQDVLGLHFFSPANVMRLLEVVRGA
                     RTAKDVMATAMAMAKRIRKTAVVSGVCDGFIGNRMIEQYSRQAGFLLEEGALPQQVDR
                     AMEAFGFAMGPFRMGDLAGNDIGWAIRKRRYLEQPGMVYPKNGDLLCEMGRYGQKTGK
                     GWYDYVPGDRTPHPSAEVEAMIVAQSRELGLERRAISDKEIVERLVYALTNEGARLLE
                     EGIAARASDIDIVYLSGYGFPVWRGGPMFHAEQVGLYNVLASMRGYARGHRGECWEPA
                     PLLVELADAGLSFNDFAARKRTAA"
BASE COUNT         1813 a         3596 c         3248 g         1785 t
ORIGIN      
        1 cttaactcag gcgttaggca gcagccgcca tgcagtcgtc gctgctcggg gaacgccaat
       61 gtagaagacc gcacttcttt gcgtgttggt cgcgacaagc ctgctcaggc ttttccttct
      121 catacgccac ttcaagcgag acctgacacc tgaagacaag ataattggcg tcatgctgct
      181 atttgtccca gtggcaggtc cgttgctcta ctcgccttct taatgatctg ccagctcagc
      241 ataagatcct gcagaatcgc ggcggtcgcg gctcgtacac gcattcctgg atttcagtca
      301 agccgatcgc ggacaagatc gtcaaggaca agcgtgagtc gttggacaag aatgatcgcc
      361 aatgaacgca agctactgcc taacaattcg tcaaagccga gacagcttcg tggctcggct
      421 tgaatggtga cgtgtacctt cacgccaagc cgctttgctg accagcttga ctcaggctcg
      481 tcccacgccg tttgcagtac cggcaccaac tcctcagggc gctccatctc ccacgaggca
      541 ggtctcggac atgcaccatg gacgaagttc ggatattcgg cgtcaggacc cgattccgga
      601 atcgcgatca ccgctggtgc tctcgagaag ccagagttcc ctgctggacc ctgttccaac
      661 acgatcatca agtatgtccg ttgaacagac gtgttctggc acaagggcag ggacacgagc
      721 cgaactacgc cgctggtggt gctacaggac tggttagccc ggactttccg aattgtcttt
      781 tgacatgcgt tacaggcccg gcgacgatcc acgcgggaag accccgcgcc acgacgtcgg
      841 ccatattcct ggatcacgcc tggagtcaac aggacattcc accttcggaa catcgagaga
      901 caccgggcgg aattcccctt ccggtttcgg cacgcacgca tgacctcatt gaatgggatg
      961 caatcgcctg ccgaacgact cttcccggta gcgcttcaga tgcagcagtc ttggccagcg
     1021 gggcattctt gagcatcaat tgggcgcagc cctgaattct ctatccagag agcggaaggc
     1081 atgccggggc atcgctaacc gcgcaacgtg tcacgcagca tgtggcctcg tttgcgccgg
     1141 ccaaggagag gacgcccttg acgctgggtg gattgactta cccgggctgg gaagtacgcg
     1201 ggcgttgatc cagaattccc tcggcgaggg cgtatcgtgc gccgataccc gtacttcttt
     1261 cgaaatcatg cggtcctgga ggagatgtcc atgcaacgtg ccaccggaat cggcggaatc
     1321 ttcttcaagt ccagggatcc gaaagcgctg ggtgcctggt accgtgacca cctcggcctc
     1381 gatgtgacgg actggggcgg cgtggtcttc cagtggggtg gccccggcag cccgccgggc
     1441 atgacgctct ggagcccgtt cgcgcaggac acggactaca tggcgccgag cacctcgagc
     1501 ttcatgatca acttccgcgt cgacgacctc gatgcattgc ttgaagctct tcgacaagaa
     1561 ggctgcaacg tcgtggacaa gaccgattcc tccgaccaag gcaagttcgg atgggtgatc
     1621 gatcccgagg gcaacaaggt cgagctctgg cagccacccg cagggcaatg agcccaggcg
     1681 atcgacacga ctcatcatcc accatcaccg cagagggcgt tccatcacga tgaactcgca
     1741 tccgatcgag acctggcacc ggctcgtcgc gaccaacgat ccctcgggac tcgccggact
     1801 tctggccgat gacgcggtct tcctctcgcc gatcgtgcac acgccacagc atgggaaggc
     1861 gctcgtcacc gcctatctgt ccgccgcatt ccaggtgttc ttcaacccca ccttccacta
     1921 cgtgcgccag atcctgggcc catcggatgc catgctggag ttcgaggccg aaatcgacgg
     1981 cgtgctggtc aacggcgtcg acatcgtcaa gtggaacggg gccggccaga tcatcgagtt
     2041 caaggtgatg atccggccac tcaaggccgt caacctcatc cacgaacgca tgggcgcgat
     2101 actggcggcc gcccgccgaa cggggtgatc gctgccgttc gcttcacgca tccagggtgc
     2161 gaccagcccg ggcagctacg cagcgcgtga ttgcgcgagc ttgcgatagt gcgccaggtg
     2221 ttcctcggtg ctacccatgc gcagctcgaa cgcgagcagg cgcttgaagc aatggctgac
     2281 accaagttcg tcggtcatcc ccatgccgcc gtgcaactgc accgcttcct gccccacctt
     2341 gcgcgcggcc tggccgacaa ggaccttcgc gccgctcacg gcttcgcgtc gctcgtgcgc
     2401 atcggactgc aggcaggcgg acgtggccag gtagaccatc gaccgcgcct gctcgacctg
     2461 catgtacatg tccgccatcc ggtgctgcaa tgcctgcagg cggccgatcg cggtgccgaa
     2521 ctgcacgcgg tcgcgggcgt actgcacggt ggcggcaagc acgcgatcca gcgcgccaag
     2581 cgcatccgcg caccatgccg cgataccgcg atccagcgct tcgtcgatgg cctccctggc
     2641 atccacgtcg ccaagacgtg aatcggcggc gaccatgagg tcgagttcga tatcggcggc
     2701 gcgcatgcca tcgactgtcc ggtagtcgcg cagatcgacg cctgccgtgt cacggtcgac
     2761 cacgaacaca ccgacgcctt catcgacacg cgcactcacc aggagccacc gcgccatggt
     2821 ggcgtggtag acgcaggtct tgcgcccctg caatcgccag ccatcctccg agcgcgtggc
     2881 gatggtcgag cacggcgcga accagtcgcc accctcgtcg tgcgcgaaca ccgcgatggc
     2941 ctcgccggac gccaacgctc ccagcagccg ctcgcgttgt gtatcccgtg ccagggccga
     3001 aaccatccgc gtagccagta ccgcactgga atggaacggc tcgaccagca agccggcgcc
     3061 gatcgcctgc gcggcaagca tcatgccgac cggacccgcg ccgatgccgc catcggcctc
     3121 gtcgatgtcc agcgccagca ggccgaggtc ggccagctcg gtccaggtcg ttgcggacca
     3181 tcctgactca ctggcgagga tcccggcacg atgctcaatc gtgtagctcg attccagata
     3241 acggcggatc gagtcctgca gcagttgctg ctcgtcggtg aaggaaaagt ccatgggccg
     3301 ccttcgtcag agttgcagga ccatctgcgc gatgatgttg cgctggatct cgttcgatcc
     3361 accgtagatg ctcaacttgc gcaggttcat gtactgggcg gcgacccgcg gcacgtcttc
     3421 gccagcgccg tcgaactgca aggcgcgcga acccaacgcc tcgacctgca gttcgctgat
     3481 gcgctgcagg ctctcggtcc cacggatctt caacatcgag gcttccgggc cgggggcctg
     3541 ctgccgcgcc tcggcgaaga tcacccgcag gttggtgact tccagtgcct tcagttccag
     3601 ctccacttgg gcaacacggg tcgcgaagat cggatcttcg agcagggccg ccccgttgcg
     3661 cgtcgcctcg cgggccatct gcttgagccg cgcgagctcc cgacggctgg cgccgatgcc
     3721 ggcgatgttg gtgcgttcat ggccgagcag gaacttcgcg taggtccagc cggcgttctc
     3781 ctcgcccacc cggttctcga ccggcacgcg gacgtcgtcg aagaagatct cgttgacctc
     3841 gtggcggcca tccagcagtc gcgtcggatg cacgcggata ccgggcgtct tcatgtcgat
     3901 cagcaggaag ctgatgccgc gctgcggctt ggccgccgga tcggtgcgca ccaggcagaa
     3961 gatccagtcg gcgtgctggc ccagcgtgtt ccaggctttc tgaccattga cgacgtagtg
     4021 gtcgccgtcg cgctgcgcgc gggtcttcag cgatgccagg tccgaaccgc tgccgggttc
     4081 ggaatagcct tggcaccacc agtcggtgcc gtcgaggatg cgcggcagga aacgcgcctt
     4141 ctgcacctcc gacccatagc gcatgatgac cggcgcgacc atcttcaggc cgaacggcaa
     4201 ctggatcggc gcatcggcgg caaagcactc gatgtcgaac aggtactgcc gggtcttgtc
     4261 ccaaccagtg ccgccgaatt cggccggcca catcgccgcg ccccagccgc gctcgaacag
     4321 gatgcgttgc cagcggcgca cgtcgtcggc cagtaccgac tcgtcgccgc ggcgcacgcg
     4381 atcccggatg tcatcgggga ggttcgcggc aaggaaggcg cggacgtcgt cgcggaacag
     4441 gacttcgtcg gcgctgaaat tcaggtccat gggaactcca cgcgatcagg acgcgcgcaa
     4501 cgccgcggcc agttgctgcc gcacctgcgg gatcgcggca tacggatcgg cgggtgcgcc
     4561 gccggagagc agcgcatcgg cgcgttcgcg aacgccttcg acggcctgcg ggtgcggcag
     4621 cacccagaaa cgatcgtggg cgatggcatc gaaggtcagg cgcgcgacgt cgtccgcagt
     4681 gaccttggcc gaggcgatgg ccttctccac catcgaggca cccaacgccg ccgcgcgcga
     4741 acgggtcggg tcgtcatgcg ggcggttgcg atgcgacttc gcgatacccg tcgcgaccca
     4801 cgacgggcac aacaccgagc aaccgatctg gtccgtgacc agggacaggt cctggtgcag
     4861 cgtttccgaa agcgacacca ccgcatgctt ggcgacgttg tagggcccca tcaacggcgg
     4921 gtcgatcagt cccgccatcg aggcggtgtt gatcacgcgt ccgcggtacg ccggatctgc
     4981 cttcgcattg gccagcatgc gcggcacgaa ggcacgcacg ccgtggatcg tgccccacag
     5041 gttgacgccc agcacccatt cccagtcgcg cgtggtggct tcccagagca ggccggtgca
     5101 gccgacgccc gcattgttga acaacaggtg cacggcgccg aagcgggtga atgcatccgc
     5161 cgcgagtgct tccatggcca cgccatcggt cacatccagc gtgcgctgca ggatatcgat
     5221 gccgtcgagt ccggcgaccg tctccgccag ggcatccgcc tgcacgtcga ccagcaccag
     5281 cgacatgccc tcctcggcgg cgatgcgcgc gaattcgcga ccgaagccgg aacccgcgcc
     5341 tgtgatcacg gcgaccttgc cgcgtagcgt ctcgatcttc ataccgcggt gtacccgcca
     5401 tcgaccgcca gcacctggcc ggtgatgtgc tggccggcat ccgaggcgaa cagcaacgcc
     5461 gcgcccttca ggtcttcgtc gccgcccagt cgacgcaacg gcgcgttgcc catcagcgcc
     5521 tcttcgccga ctgccgcgat cacgccctgg ctcatcttcg acgggaagaa gcccggtgcg
     5581 atcgcattga cggtgacgcc gcggccaccc cactccgccg ccagcgcgcg agtgagattg
     5641 accaccgcac ccttggacgc gttgtaggcg gcggtcttca gcaggccacc tgcattgccg
     5701 cgcaacccgg ccaccgacgc cacgttgatg atccggccac tgccgcgagg cagcatcgaa
     5761 cgcttggcga cctgctggga cagcgcgaac aaaccacgca cgttgagatc gaacaccttg
     5821 tcccaggcct ccagcggata gtcctctgcc ggtgcacccc agctggcccc ggcgttgttg
     5881 accagcacat cgacctggcc gaacgcggcc agcacgctgt ccaccagctg cgtcaccgcg
     5941 tccggcttgc tggcatcggc ggcgaaggct tcggcacgga taccacgcgc acgcaagtgt
     6001 tccagtgcgg cgtccagctc atgttgcttg cgcgcggaga tcgcgatggt cgcgccgtag
     6061 tcacccagcg cttcggcgat ctgcaggccc aggccgcgcg agccgccggt gatgagggcc
     6121 acgcgcccgt cgaggtcgaa caaggtcttg ttgccgtgca tgtccactcc ttcagaacca
     6181 gtcgtcgcgc atcgccagcg cggtgtcgtc gttgtcggcc agccggtcca gccacgcatc
     6241 cgcgcgcggc agttcccact gcatgaaata gcgcgccgcc tgccgcttgc cacgggcgaa
     6301 atcgtcgtcg cggtcgccga gcgcaagcac ctgctccagc cagatccacg ccacggtgat
     6361 gtggccgaag gcctccaggt acgcgctggc attggccagg cgcgccatcg gatcggcgac
     6421 ccgcagcagc gaggcagtaa catcgcgcag gcgcgtcagg cgaatcgaca aggcatcggc
     6481 aagcgccgcg gatgatgtgt cgagggcacg cgtgatgctc gcatcgagca acgcgccatg
     6541 cgccgcaaac gccgcgccat cgaacatcgc caccttgcgg ccgagcaggt cgatgccctg
     6601 gatgccatgc gtgccttcgt ggatcgggtt gagccggttg tcgcgccaca gctgctcgac
     6661 tttgtagtcg cgcgtgtagc cgtaaccgcc atgcacctgg atggcgaggt cgttcccggc
     6721 caggcaccac tgcgacggcc agctcttggc gatcggggtg aggatgtcga gcagcagttc
     6781 tcgcgatgag acgagcgccg catcaccggc ctcacgcgcg atgcgcgctt cgtccaccag
     6841 cctggcgcag taaagcacca ggcccagccc gccctcggca taactcttct gcgccagcag
     6901 catgcgccgc acgtccgcat gcgcgaccag cggctgttgc ggttgcgacg gatccttgcc
     6961 cgccggcccg ggcaggcgcc cctgcggccg gttgcgtgcg tagtccagcg caagcaggta
     7021 gccggcgtag cccagcgcca ctgctccgag gccaacaccg atccgcgcgt cgttcatcat
     7081 gtggaacatg ccggccaggc cctcgcccgg cttgccgacc aggtagccga ttgcgccggc
     7141 gcgtccttcc ggcttgaagc gtgcttcgcc gaagttcagc gcgcaattca ccgtcgcccg
     7201 atagcccagc ttgtggttca ggccggccag gctgacatcg ttgcgttcac cgacattgcc
     7261 atcggcatcg accagcgaac gcggcacgat gaacaatgag attccgcgcg tgccagccgg
     7321 caacttgcca tcggcgtccg gcactttcgc cagcaccaga tggacgatat tgccgaggat
     7381 ctcgtgctca ccgccggaga tccacatctt gcggccgctg atgcggtact gcgggccgaa
     7441 tggtgattcg cgctcgtatt ccgcgcgtgt ggtgatgtcg gacagcgacg atcccgcctg
     7501 cggttccgac agacacatcg tgcccgtcca gtcgccgcgc agctgcggca tcgcgaaggc
     7561 gtcgacctgc gccggcgtgc cgtgcgcgag caacaagcgg gcattgccca tcgtcaacaa
     7621 cggatacgcg caggccccga tgttggcggc gtagagattc accagcgccg ccttttccac
     7681 caccaccggc aactgcatgc cgccgacgga ttcgtcctca gtcgccgaca ccagcccgct
     7741 gacggcgaac gcgcgcaccg cctcggcaat ttgcggatgc acgctgacat tttcgccatc
     7801 gaagtgcggt tcatcgcgat ccgctgcctg gtagcacggt gcgaacacct cggcggccaa
     7861 tcgatcgcaa ccgtccatca ccgcatcgaa cgtctccgcg gaatgcgcgg cgaagcgcgg
     7921 tgcgtgcagc agcgcatcga cgcgcaacca gtcatgcagc tggaacttca gttcgcggcg
     7981 cgggatcagc ggcatcgaca tcgatcaggc gacctcgaac aaaccggccg cgccctgtcc
     8041 gccgccgacg cacatggtca cgaccacgta cttcacccca cgccgtttgc cttcgatcaa
     8101 cgcatgcccg accaggcgcg cgccactgac gccataggga tggccgaccg cgatcgcgcc
     8161 gccgttgacg ttgagccggt cgtgcgggat gccgagtctg tccatgcaat acaggacctg
     8221 cacagcgaac gcttcgttga gttcccacaa gccgatgtcg tggacactca ggccggcgtt
     8281 cttcagcagc tttggcaccg cgaacaccgg gccgatgccc atctcgtcgg cttcgcaacc
     8341 ggccaccgcg aaccctcgga agatgccgag cggctgcaag cccttgcgcg aggcagcatc
     8401 ggcggacatc agcacgctgg ccgacgcacc atcggagaac tggctggcat tgccggccga
     8461 gaccacgccg ccgggcactg cgctgcggat cttcgctacc gcgtcgtagg tggtatccgc
     8521 gcgcacgcct tcatcgcgat ccagcgtgac ctcgcgcgtg ctgacggccc tggtcaccgg
     8581 atcggcgact gccatccgaa ccgtgatcgg cacgatctcg tcgtcgaagc ggccggcggc
     8641 ctgcgccgca gcggcacgca cctggctttc ggtgccgtaa cggtcttgcc gttcgcggtc
     8701 gatgccgtaa cgtttcgcca cgatctccgc ggtctgcagc atcggcaggt agattgccgg
     8761 atagcgccgc ttcagccagg tgtcttccag catgtgctgg ttgatcgtgg cctgcacggt
     8821 ggagatgcat tccacgccgc cagcgacgaa cacctcgccc tcgccggcga tgatgcgctg
     8881 cgcggtctgc gcgatcgcct gcaggccgga ggcgcagaaa cggttgacgg tggcaccggg
     8941 cacgctggcc gggagcccgc cgcgaatcgc gatctgccgg gcgatattgc tgccggttgc
     9001 gccttccggc gtagcacaac ccatcaccac gtcctcgacc tcggcgggat cgatgccggc
     9061 gcgttcgacg gcatggcgga ccacgtggcc gccaacggtt gcgccgtggg acaggttcaa
     9121 tgcgcctttc cagctcttgg ccagcccagt gcgcgcggtg gagacgatga cggcttcttt
     9181 cacggcgggc ctcacgcggc agtgcgcttg cgcgcggcga aatcgttgaa ggacaggccg
     9241 gcgtcggcca gttcgaccag cagcggcgcc ggctcccagc actcgccgcg atggccgcgt
     9301 gcgtagccgc gcatcgacgc cagcacgttg tacaggccga cctgctccgc atggaacatc
     9361 ggcccgcccc gccagaccgg gaagccgtag ccggacagat agacgatgtc gatgtcggac
     9421 gcacgcgcgg cgatgccctc ctcgagcaaa cgcgcgccct cgttggtcaa tgcgtacacc
     9481 agccgttcga cgatctcctt gtcgctgatc gcgcgacgct cgaggccgag ttcgcgtgac
     9541 tgcgcgacga tcatcgcctc gacctcggcc gacggatgcg gagtacgatc gcccggcacg
     9601 tagtcgtacc agcccttgcc ggtcttctgg ccatagcggc ccatttcgca gagcaggtcg
     9661 ccgttcttcg gatagaccat gccgggctgt tcgaggtaac gacgcttgcg gatcgcccag
     9721 ccgatgtcgt tgccggccag gtcgcccatc cggaacggcc ccatcgcgaa accgaacgcc
     9781 tccatcgcgc gatccacctg ctgcggcagc gcgccttcct caagcaggaa gccggcctgg
     9841 cgcgaatact gttcgatcat gcggttgccg atgaagccgt cgcacacccc ggacaccacc
     9901 gcggtcttgc gaatgcgctt ggccatcgcc atcgcagtgg ccatcacgtc cttcgcggtc
     9961 cgtgcgccgc gcaccacttc cagcaggcgc atgacattgg ccgggctgaa gaagtgcaag
    10021 cccagcacgt cttgcgggcg cttggtgaac gccgcgatcc ggtccacgtc gagcgtcgac
    10081 gtgttggtgg ccaggatcgc gccgggcttg gctacggcat cgatcttctc gaagaccgat
    10141 tgcttgaccg catagtcctc gtacaccgct tcgatcacca ggtcggcatc ggccagtgcg
    10201 tcgtaggaga gcgatggtgt caccagcgcc atccgctgct cgacctgcgc atcggtcaac
    10261 ttgcccttct tcgcgctggc ctcgtaattt cggcggatgg tggccacgcc gcgatccagc
    10321 gcctcctgcg tggtctccac cagcaccacc gggatgccgg cattgaggaa attcatgctg
    10381 atgccgccgc ccatggtgcc ggcgccgatc acgcccacct tgtcgatcgc gcgtggcggt
    10441 at
//
LOCUS       DAOOCX010000084        10161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_6237_length_10161_cov_7.663170, whole genome shotgun sequence.
ACCESSION   DAOOCX010000084 DAOOCX010000000
VERSION     DAOOCX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6237_length_10161_cov_7.663170"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PK399_08600"
     CDS             complement(<1..1139)
                     /locus_tag="PK399_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW13217.1"
                     /translation="MDSLLKQFAQTSQLGANGAYVEDLYEQYLVAPDSVDAKWRQYFD
                     GLKGREAGDIPHSAVIEQIAQAGRLAARGVVTAASGVGDERERGVGKLITAYRSRGHL
                     AANLDPLGLTPKPEAPDLALGFHRLSESDTGSEFSTGGVAGRERMKLGELVNLLKATY
                     AGSVGAEFMHITDAEQRRWMYERLEAAGGNYGRTAADKQRILERLTAAEGLERYLHTK
                     YVGQKRFSLEGGDSLIPMLDVMIQRAGGDGVKDIVIGMAHRGRLNVLVNTMGKPPRKL
                     FDEFEGKFEHSRAGDHAHTGDVKYHMGFSADIATPGGPVHLALAFNPSHLEIVDPVVV
                     GSVRSRQLRRNDKERKQVLPILLHGDAAFAGQGVVMELFQMSQARG"
     gene            complement(1292..2215)
                     /locus_tag="PK399_08605"
     CDS             complement(1292..2215)
                     /locus_tag="PK399_08605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW13218.1"
                     /translation="MTPDDPRLQPYRVDVVGFDAALAELRSVRDEVFVGEQGVPITLE
                     HDALDPLCTHVLARLLDGSPVGTARLTPDRKIGRMAVRAAWRGRGIGDALLRALLDDA
                     RKRGWPAVRLNAQVGAIGFYARHGFQPEGERFMEAGIEHQAMVLAFTGPARIDSREAG
                     IDASIGIIERARRTLWIRSVALDPWLYDDGNVLQALRGFATRGGGQQVLVLLHDAGAP
                     QQAHAPLLALAQRLPSIFVFRELQDPVDRNDPAAWIAGDGGGYYLRGLGERVQGEAEQ
                     DAPAKVRQLATRFDDAWQRSRQVSEYRALGL"
     gene            complement(2212..3393)
                     /locus_tag="PK399_08610"
     CDS             complement(2212..3393)
                     /locus_tag="PK399_08610"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW13219.1"
                     /translation="MIEVHATSKHFLGMPAAEFLRDYWQKKPLLVRNAFPGYQSPLQP
                     EDLAGLACEDGVLARLIEHDATSDGWRVRHGPFAETDFPGLPDHDWTLLVQDMDKWDA
                     DLRALLDRFDFLPRWRIDDVMVSFAATGGSVGAHVDQYDVFLLQAQGHRRWQIDASDA
                     PPLAFRDDVELKLLRTFNPSHDWVLAPGDMLYLPPGVPHHGIAQDPCLTFSVGMRAPA
                     VAELLGDYVDALAADAPDALRYADPDLSPPKDANEIDDAAMQRVVEALRQLRMDDPDR
                     FGDWFGGFVTTYRSAVQPAADDTPRPRIEVEWDLGRGAILQRHPWSRMAWRRASKGAR
                     LYAGGQAHAMPVRDAQRLAAANEVDGLLYDSLSAPGRDVVFALLEGGHYRLDTGEDDD
                     E"
     gene            complement(3390..4319)
                     /gene="bla"
                     /locus_tag="PK399_08615"
     CDS             complement(3390..4319)
                     /gene="bla"
                     /locus_tag="PK399_08615"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HPW13220.1"
                     /translation="MKAFIASLLATSLLGACQILPPAPHAPAKTPAGVAQPSLDKVPA
                     ATPCKDQDDWNEATAPRHVFGNTWYVGTCGISVLLVATPEGHVLIDGATAKAAPHVLA
                     SLHALGVDPRDVRVLLSSHEHNDHVGGLAQLQAATGAPLLARKTAMPALRLGRGDRSD
                     PQFGIHEGFPPIADVRELVDGGISLGGLRISAHAMPGHTPGATTWSWDSCDGGQCRHM
                     VHADSISAISDDTFRYSDHPDYVAAFRAGLDTLAALPCDILMTPHPGGSDLWPRLRGE
                     APLVDTNACRAYADQGREGLATRLDKERKGTAP"
     gene            complement(4322..5686)
                     /gene="purB"
                     /locus_tag="PK399_08620"
     CDS             complement(4322..5686)
                     /gene="purB"
                     /locus_tag="PK399_08620"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPW13221.1"
                     /translation="MSDALTALSPLDGRYAGKVDALRPIFSEYGLIRARVKVEVEWLL
                     ALAAEPGIPELVPFSDAAAARLRALAQGFSVEDAARVKAIEATTNHDVKAVEYLIKER
                     LQDDAELGPALEFVHFACTSEDINNLSYALLLSKARQHVLLPRLDALIATLRGMAHAH
                     AAMPMLSRTHGQTASPTTVGKELANVVARLQRQGEALAALPMPGKINGAVGNYNAHVA
                     AYPDIDWPAFSQRFVESLGLDWQPYTTQIEPHDGIAELCDACKRIDTIAIDLCRDAWG
                     YISQGYFKQALKAGEVGSSTMPHKVNPIDFENAEGNFGIASALFEHFAAKLPVSRWQR
                     DLTDSTVLRALGTAFGHMLIGFDSLQKGLGKLSVNPERLAADLDASWEVLAEAVQTVM
                     RRHGLPNPYEQLKALTRGQGITQASMRDFIATLDLPAADKQRLLEMTPGSYTGLAEQL
                     ARDA"
     gene            5766..7178
                     /locus_tag="PK399_08625"
     CDS             5766..7178
                     /locus_tag="PK399_08625"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014089811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HPW13222.1"
                     /translation="MATRKASGFRIEHDSMGELRVPAKALWGAQTQRAIDNFAISARP
                     MPAAFIRALALTKAAAAVVNGHLGLLARAQADAIVEAATAIAGGAHAGQFPVDRYQTG
                     SGTSSNMNANEVIAHVAAAASGQSIHPNDHVNLGQSSNDVVPTALRVMAVQGARQDLL
                     PALRHLRKTIDKRAKALGRIVKTGRTHLMDAMPLTVAQEFGAWSAQLDSAQARIEDSL
                     KRVRRLPIGGTAIGTGINAHPKFGKGVAKALSVATGAKFEQASNTFEGLAAQDDLVEL
                     SGQLNALAVALMKIGNDLRWMNSGPLAGLGEIELPALQPGSSIMPGKVNPVIPEALCM
                     ACAQVMGLHQAISIAGQSGNFQLNVMLPLIACDIDESLQLLSSAMTALADKALAGLQV
                     RKDTMASALDRNPILVTALNPVIGYEQAAKIAKRAYAEGRPVLDVALEDSGLDEKTLR
                     RLLDPADLAKGGINAGTSSS"
     gene            complement(7250..7939)
                     /locus_tag="PK399_08630"
     CDS             complement(7250..7939)
                     /locus_tag="PK399_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13223.1"
                     /translation="MKHIPGLTLVLLAGMSLAACSQPPAPPAPPAPPAPPVASGNDTF
                     IGRHVGSAIEQARKELATQNISISDGMNINVNGRQWNTGDSSLPKAEITPKGDLLIEG
                     KAVAITAAQRADLLAYRGQIIGIAEAGMTIGAQGANIAGHAVSGAIGAIFGGKDGEKE
                     FEKKMEAEAAKIEAEAMKLCTRLPALMAGQQALAASLPEFKPYARMTQAEIEDCGKDV
                     GKHKGVSVTSS"
     gene            complement(7955..8623)
                     /locus_tag="PK399_08635"
     CDS             complement(7955..8623)
                     /locus_tag="PK399_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13224.1"
                     /translation="MKTRNLLAIALLATAPLFTAQAAGRDTGIGSEIRQEMANAREEM
                     RIELAKARQELDTGNLRLDDGFQFTAHDGKKSKRAKAELAPAEITPQGDLLIDGKVQA
                     INDGQRRQLLAYRGQVITVAKAGIDIGQRSADAALEAVGSGSWVGLLVGAMTGNLERR
                     IERVVKQEIEPGMRSICRQLPAVLESQQRLSANLPQFRPYATLDADDVANCERDIRTE
                     FASR"
     gene            complement(8651..9682)
                     /locus_tag="PK399_08640"
     CDS             complement(8651..9682)
                     /locus_tag="PK399_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13225.1"
                     /translation="MDVATPRTPRPAPHATHKFKLLLKREFWEHKGGFLWAPVWAGAI
                     SLLLTLMALIVGEVAARKALASGKMQIDGDVTINGLDLGVLTSKMDPDDLRKLADGID
                     FSMLMSGVWPMVVLGFVVFFYCLGSLYDERKDRSVLFWKSLPLSDRDTVFSKAASALL
                     VAPTLAIGAAIACMFAFMLLVSAFVLLHNGNPISLLWGPGNPLANAGLLLASIPIYAL
                     WALPTVGWLMLCSSWSKSMPFLWAIMIPVFAGIFISWFEVMNVFDLQSGWFWKNIVAR
                     SLLSVFPGTWVDVMDVGAIAKGNAPSHAMVLKSTYAVLATSQLWIGAAAGIAMLVAAV
                     RMRRWRDDN"
     gene            complement(9706..>10161)
                     /locus_tag="PK399_08645"
     CDS             complement(9706..>10161)
                     /locus_tag="PK399_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW13226.1"
                     /translation="VQLHLALVMAIDAKLLVLDEPTLGLDILYRKQFYQRLLEDYFDE
                     DKTIIVTTHQVEEIEHILTDVMFIRDGKVVLDAPMDTLAVRYTEVLVDAGKQDEARAL
                     KPIDERALPFGKTVMLFDGTDPARLAALGETRTPGLADLFVATMKGTYA"
BASE COUNT         1555 a         3353 c         3552 g         1701 t
ORIGIN      
        1 ccgcgcgcct gcgacatctg gaacagttcc atcaccacgc cctggccggc gaacgcggca
       61 tcgccgtgca gcaggatcgg caggacctgc ttgcgctcct tgtcgttgcg gcgcagttgg
      121 cgcgaacgca cgctgccgac caccaccgga tccacgatct ccaggtgcga cgggttgaac
      181 gccagcgcca ggtggaccgg accgccgggg gtggcgatgt cggcggagaa gcccatgtgg
      241 tacttcacgt cgccggtgtg cgcgtggtcg ccggcgcggc tgtgctcgaa cttgccttcg
      301 aactcgtcga acagcttgcg cggcggcttg cccatcgtgt tgacgaggac gttgagacga
      361 ccgcggtggg ccatgccgat cacgatgtcc ttgaccccgt cgccgccggc gcgctggatc
      421 atcacgtcca gcatcgggat caggctgtcg ccgccttcca gcgagaagcg cttctggccg
      481 acgtatttgg tgtgcaggta gcgctcgagc ccctcggccg cggtcaggcg ttcgaggatg
      541 cgctgcttgt cggcggccgt gcggccgtag ttgccgccgg cggcttccag gcgctcgtac
      601 atccagcggc gctgttcggc gtcggtgatg tgcatgaact cggcgccaac gctgccggca
      661 taggtcgcct tgagcaggtt gaccagctcc cccagcttca tccgctcgcg accggccacg
      721 ccgccggtgc tgaattcgct cccggtgtcg ctttcggaca ggcgatggaa gcccagcgcc
      781 aggtccggcg cctcgggttt cggcgtcagg cccagcggat cgaggttggc ggccaggtgc
      841 ccgcgcgaac ggtaggcggt gatcagcttg ccgacgccgc gctcgcgctc gtcgcccacg
      901 ccgcttgccg cggtgaccac gccacgcgcg gccagccggc cggcctgggc gatctgctcg
      961 atgacggcgg agtgcgggat gtcgccggct tcgcggccct tgagcccatc gaaatactgc
     1021 cgccacttgg catcgacgct gtccggggcg accaggtatt gctcgtagag atcctccaca
     1081 taggcgccat tggcaccgag ttgggaggtc tgggcaaact gcttcaggag actgtccacc
     1141 atggcgtggc tagcggcctt gcgataagtg attgatccgg atgaagttgc catccggtgc
     1201 gggcccgatg aatgggcggg cgaaaggatc ggaattatag ccggagcggc ttgcgcaccg
     1261 catcacatcc cttcgtcgaa ggggcagggg ttcacagtcc gagcgcccgg tattcgctga
     1321 cctggcgcga acgctgccag gcatcgtcga agcgcgtggc cagttgccgg accttcgccg
     1381 gggcgtcctg ctcggcctcg ccctgcacgc gttcgccgag accgcgcagg tagtagccgc
     1441 cgccatcgcc ggcaatccac gcggccggat cgttgcggtc caccggatcc tgcagttcgc
     1501 ggaacacgaa gatgctgggc aggcgctggg ccagcgccag caagggcgcg tgtgcttgct
     1561 ggggcgcgcc tgcatcgtgc agcaacacca gcacctgctg gccgccaccc cgggtggcga
     1621 acccgcgcag ggcctgcaac acgttgccgt cgtcgtacag ccagggatcc agcgcgacgc
     1681 tgcggatcca cagggtgcgg cgcgcacgtt cgatgatgcc gatggacgcg tcgatcccgg
     1741 cttcgcggct gtcgatccgg gccggaccgg tgaacgccag caccatcgcc tggtgttcga
     1801 ttccggcctc catgaagcgc tcgccctcgg gctggaagcc atgccgcgcg tagaagccga
     1861 tcgcaccgac ctgcgcattc aaccgcactg ccggccagcc gcgcttgcgc gcgtcgtcca
     1921 gcaatgcgcg cagcagtgca tcgccgatcc cgcgcccgcg ccaggccgcg cgcacggcca
     1981 tccggccgat cttgcggtcg ggggtcagcc gcgcagtgcc gaccggactg ccatcgagca
     2041 ggcgggccag cacatgggtg cacagcggat ccagtgcgtc atgctccagg gtgatcggca
     2101 cgccctgttc gccgacgaag acctcgtcgc gcacgctgcg caactcggcc agcgcggcat
     2161 cgaacccgac cacgtcgacg cggtacggct gcaggcgcgg atcgtccggg gtcattcgtc
     2221 gtcgtcctcg ccggtatcga gccggtagtg gccgccttcg agcagggcga acaccacgtc
     2281 gcgaccgggc gcggacaggc tgtcgtagag caggccatcg acctcgttgg ccgccgccag
     2341 ccgttgcgcg tcgcgcaccg gcatcgcatg ggcctgcccg cccgcataca ggcgcgcgcc
     2401 cttcgacgcc cgccgccatg ccatccgcga ccatgggtgg cgctgcagga tcgcgccgcg
     2461 gcccaggtcc cattcgacct cgatccgtgg ccgcggggtg tcgtcggcgg ccggctgcac
     2521 ggcgctgcga taggtggtga cgaagccgcc gaaccagtcg ccgaagcgat cgggatcgtc
     2581 catccgcaac tggcgcagcg cctcgaccac gcgctgcatc gcggcgtcgt cgatctcgtt
     2641 cgcgtccttc ggtggggaga ggtcgggatc ggcgtagcgc agcgcgtccg gcgcatcggc
     2701 ggccagcgca tccacgtaat cgcccagcaa ttccgcaacc gccggcgcgc gcatgcccac
     2761 cgagaaggtc aggcagggat cctgggcgat gccgtggtgg ggcaccccgg gcggcaggta
     2821 gagcatgtcg cccggcgcca gcacccagtc gtgcgaggga ttgaatgtgc gcagcagctt
     2881 caattcgacg tcgtcgcgga acgccagcgg tggtgcgtcc gaggcatcga tctgccagcg
     2941 ccgatggccc tgcgcctgca gcaggaatac gtcgtactgg tccacgtgcg cgccgaccga
     3001 gccgccggtc gcggcgaagc tgaccatcac gtcgtcgatc cgccagcgcg gcaggaagtc
     3061 gaagcggtcg agcagggcgc gcaggtcggc gtcccacttg tccatgtcct ggaccagcag
     3121 ggtccagtcg tggtcaggca ggccggggaa gtcggtttcc gcgaacggtc cgtggcgtac
     3181 gcgccagccg tcgctggtgg cgtcgtgctc gatcaggcgc gccagcacgc cgtcctcgca
     3241 ggccagcccg gccaggtctt ctggctgcag cggcgactgg taaccgggaa acgcattgcg
     3301 gaccagcagc ggcttcttct gccagtagtc gcgcaggaac tcggcggcgg gcatgccgag
     3361 gaaatgcttg ctggtggcgt ggacttcgat catggcgcgg ttcccttgcg ttccttgtcg
     3421 agccgtgtcg caaggccctc gcggccctgg tcggcatagg cgcggcaggc gttggtatcg
     3481 accagcggcg cttcaccgcg caggcgcggc cacaggtcgc tgccgccggg atgcggggtc
     3541 atcaggatgt cgcagggcaa ggcggccagc gtgtcgagcc cggcgcggaa tgcggcgaca
     3601 tagtccggat ggtcgctgta gcggaatgtg tcgtcgctga tcgcgctgat gctgtcggca
     3661 tgaaccatgt gccggcattg gccgccgtcg cagctgtccc aactccaggt ggtcgcgccc
     3721 ggcgtatggc cgggcatggc atgtgcgctg atccgcaggc cgccgaggct gatgccgccg
     3781 tcgaccagct cgcgcacgtc ggcgatcggc gggaagcctt cgtggatgcc gaattgcgga
     3841 tcgctgcgat cgccgcggcc caaccgcaac gccggcatcg cggtcttgcg tgcgagcagc
     3901 ggcgcgccgg tcgcggcctg caattgcgcg aggccgccga cgtggtcgtt gtgctcgtgg
     3961 ctgctcagga ggacgcgcac gtcgcgcgga tccacgccga gtgcgtgcag gctggccagc
     4021 acatgcggcg ccgcttttgc ggtggcaccg tcgatcagga catggccctc gggcgtggcc
     4081 accagcagca ccgaaatccc gcaggtcccg acgtaccagg tgttgccgaa cacatggcgc
     4141 ggcgcggtcg cctcattcca gtcgtcctgg tccttgcagg gcgtcgccgc tggcaccttg
     4201 tccaggctgg gctgcgccac accggccggc gtcttcgcgg gggcatgcgg tgccggtggg
     4261 agaatctgac aggcacccag caggctcgtc gcgagcaggc tggcgatgaa cgccttcatc
     4321 ctcatgcgtc gcgggcgagc tgttcggcca ggccggtgta gctgcccggc gtcatctcga
     4381 gcaggcgctg cttgtcggcg gcaggcaggt ccagggtcgc gatgaagtcg cgcatcgagg
     4441 cctgggtgat gccttggccg cgggtcagcg ccttcagttg ttcgtagggg ttcggcaggc
     4501 cgtggcggcg catcaccgtc tgcacggcct cggccagcac ttcccagctg gcgtcgaggt
     4561 cggcggccag gcgctccgga ttgacgctca acttgcccag gcccttctgc agggaatcga
     4621 agccgatcag catgtggccg aacgcggtgc cgagtgcgcg cagcacggtg gagtcggtga
     4681 ggtcgcgctg ccagcggctg accggcaact tggcggcgaa atgctcgaac agtgcgctgg
     4741 cgatgccgaa gttgccttcc gcattctcga agtcgatcgg gttgaccttg tgcggcatcg
     4801 tgctgctgcc cacttcgccg gctttcagcg cctgcttgaa gtagccctgc gagatatagc
     4861 cccaggcatc gcggcacagg tcgatggcga tggtgtcgat gcgcttgcag gcgtcgcaga
     4921 gttcggcgat gccgtcgtgc ggttcgatct gggtggtgta tggctgccag tcgaggccga
     4981 gcgattccac gaagcgttgc gagaacgccg gccagtcgat gtcgggatag gcggcgacat
     5041 gcgcgttgta gttgccgacc gcgccgttga tcttcccggg catcggcagc gccgcgagtg
     5101 cctcgccctg gcgctgcagg cgggcgacga cgttggccag ttccttgccg accgtggtgg
     5161 gcgatgcggt ctggccatgc gtgcgcgaca gcatcggcat tgccgcgtgt gcatgcgcca
     5221 tcccgcgcag cgtcgcgatc agcgcgtcga gcctgggcag cagcacgtgc tggcgcgcct
     5281 tgctcagcag cagcgcgtag gacaggttgt tgatgtcctc gctggtgcag gcgaaatgga
     5341 cgaattccag tgccgggccg agctcggcat cgtcctgcag gcgttccttg atgaggtatt
     5401 cgaccgcctt gacgtcgtgg ttggtggtcg cctcgatcgc cttgacccgg gcggcgtcct
     5461 ccaccgagaa gccctgcgcc agcgcgcgca ggcgcgcggc tgcggcatcg gaaaacggca
     5521 ccagttcggg gatgccgggt tccgcggcca gagcaagcaa ccattccacc tccaccttga
     5581 cccgcgcgcg gatcaggccg tactcggaga agatcgggcg cagcgcatcg accttgccgg
     5641 catagcggcc atcgagcggg gacagggcgg tcagggcgtc ggacatgggg atcggcggct
     5701 gcggggatgc gcaattctaa accgcggcgg cgctatgctc ggcggatccc gcacaggagc
     5761 aggccatggc cacccgcaag gcaagcggct tccgcatcga acacgacagc atgggcgagt
     5821 tgcgcgtgcc cgcgaaggcg ctctggggcg cgcagaccca gcgcgcgatc gacaatttcg
     5881 cgatcagcgc caggccgatg cccgccgcgt tcatccgtgc gctggccttg accaaggccg
     5941 ccgccgcggt ggtcaacggg cacctggggt tgctcgcgcg cgcgcaggcc gacgccatcg
     6001 tcgaggccgc cacggccatc gccggtggcg cgcacgccgg gcagttcccg gtcgaccgtt
     6061 accagaccgg ctccggcacc tccagcaaca tgaatgccaa cgaggtcata gcccacgtgg
     6121 cggcggccgc ctccgggcag tcgatccatc ccaacgacca cgtcaacctg ggccagagca
     6181 gcaatgacgt cgtcccgacc gcgctgcggg tgatggcggt gcagggcgcc aggcaggacc
     6241 tgctgccggc gctgcgccac ctgcgcaaga ccatcgacaa gcgtgcgaaa gcgctcggca
     6301 ggatcgtcaa gaccggccgg acgcatttga tggacgcgat gccgctgacg gtggcgcagg
     6361 aattcggcgc gtggtcggcg cagctggatt ccgcgcaggc gcgcatcgag gacagcctga
     6421 agcgcgtgcg ccggctgccg atcgggggca ccgcgatcgg caccgggatc aatgcgcatc
     6481 ccaagttcgg caagggcgtg gccaaggcac tgtcggtcgc caccggcgcg aaattcgaac
     6541 aggcctcgaa taccttcgag ggcctggccg cgcaggacga cctggtggag ttgtccggcc
     6601 agctcaatgc actggcggtg gcgctgatga agatcggcaa cgacctgcgc tggatgaatt
     6661 ccgggccgtt ggccggactg ggcgagatcg agttgccggc actgcagccc ggcagttcga
     6721 tcatgccggg caaggtcaat ccggtgatcc cggaagcgct gtgcatggcc tgcgcgcagg
     6781 tgatgggcct gcaccaggcg atctcgatcg caggccagag cggcaacttc cagctcaacg
     6841 tgatgctgcc gctgatcgcc tgcgatatcg atgagtcgct gcagctgctg tcctcggcga
     6901 tgactgcatt ggctgacaag gcgcttgcag gcctgcaggt gcgcaaggac accatggcgt
     6961 ccgcactgga tcgcaatccg atcctggtga ccgcgctgaa tccggtgatc ggctacgaac
     7021 aggccgcgaa gatcgccaag cgagcgtatg cggagggacg gccggtgctg gatgtcgcgc
     7081 tggaggacag cggactggat gaaaagacgc tacgccgctt gctggatccc gccgatcttg
     7141 cgaagggcgg gatcaatgcc ggcacaagct caagctgatc atcgccccga gaagacgaaa
     7201 ggcctgtccg ggtaggcagg cccttcgttt ctctacggag gagtgccggt cagctgctgg
     7261 tgaccgacac gcccttgtgc ttgccgacat ccttgccgca gtcctcgatt tcggcctggg
     7321 tcatccgcgc atacggcttg aactcgggca acgaggcggc cagcgcctgc tgccctgcca
     7381 tcagcgcggg caggcgggtg cacagcttca tcgcctcggc ctcgatcttg gcggcctcgg
     7441 cctccatctt cttctcgaat tccttctcgc cgtccttgcc gccgaagatc gcaccgatcg
     7501 caccggacac ggcgtgcccg gcgatgttgg cgccctgcgc gccgatcgtc atgccggctt
     7561 cggcgatgcc gatgatctgg ccccggtagg ccagcaggtc ggcgcgctgc gcggcagtga
     7621 tcgcgaccgc cttgccctcg atcagcaggt cgcccttcgg ggtgatctcc gccttcggca
     7681 ggctgctgtc gccggtgttc cactggcggc cgttgacgtt gatgttcatg ccgtcgctga
     7741 tgctgatgtt ctgcgtggcc agttccttgc gagcctgttc gatcgcgcta ccgacgtggc
     7801 ggccgatgaa cgtgtcgttg ccgctggcga ccggcggggc cggtggcgcg ggcggtgccg
     7861 gtggtgcggg tggctggctg caggccgcca gcgacatgcc ggcgaggagg acgagggtga
     7921 ggccagggat gtgcttcatg atgtgttcct ccggtcagcg ggacgcgaat tcagtgcgga
     7981 tgtcgcgttc gcagttggcg acgtcgtccg catcgagagt ggcatatggg cggaactgcg
     8041 gcaggtttgc cgacaaccgt tgctgcgact ccaacaccgc aggcagttgc cggcagatgc
     8101 tgcgcatgcc cggttcgatt tcctgcttga cgacgcgttc gatgcggcgt tccaggttcc
     8161 cggtcatggc accgaccagc aggccgaccc aggatccact gccgacggct tccagcgcgg
     8221 catccgcact gcgctggccg atgtcgatcc cggccttggc gaccgtgatc acttggccgc
     8281 ggtacgcgag cagttggcgt cgctggccgt cattgatggc ctggaccttg ccgtcgatca
     8341 gcaggtcgcc ctgcggcgtg atttccgcag gcgcgagctc tgccttggcg cgcttcgact
     8401 tcttgccgtc atgggcggtg aactggaagc cgtcgtcgag gcgcaggttg ccggtatcca
     8461 gttcctgcct ggccttcgcc agctcgatgc gcatttcctc gcgcgcattg gccatctcct
     8521 ggcggatctc gctgccgatc ccggtgtccc tgcccgctgc ctgcgcggtg aacagcgggg
     8581 cagtggccag caaggcgatg gcgaggaggt tgcgggtctt catcgtcgtg tcctgtctgg
     8641 gaaaggtggc tcagttgtcg tcgcgccagc ggcgcatgcg gaccgcggcg accagcatcg
     8701 cgatgccggc ggcggcgccg atccacaatt gcgaggtggc cagcacggcg taggtcgact
     8761 tcagcaccat cgcgtggctg ggtgcattgc ccttggcgat cgcgccgacg tccatcacgt
     8821 cgacccaggt gccggggaac acgctcagca ggctgcgggc gacgatgttc ttccagaacc
     8881 agccgctctg caggtcgaac acgttcatca cctcgaacca gctgatgaag atgccggcga
     8941 acaccgggat catgatcgcc cacaggaacg gcatgctctt cgaccaggac gagcacagca
     9001 tcagccagcc gacggtgggc agcgcccaca acgcgtagat cgggatcgat gccagcagca
     9061 ggccggcgtt ggccagcggg ttgccggggc cccacagcag gctgatcggg ttgccgttgt
     9121 gcagcaacac gaaggcactg accagcagca tgaaggcgaa catgcaggcg atcgcggcgc
     9181 cgatggccag ggtcggggcg accagcagcg cgctggcggc cttggagaac acggtgtcgc
     9241 ggtcggacag cggcagcgac ttccagaaca gcacgctgcg atccttgcgc tcgtcgtaca
     9301 ggctgcccag gcagtagaag aacaccacga agcccagtac caccattggc cacacccccg
     9361 acatcaacat gctgaagtcg atgccgtcgg ccagcttgcg caggtcgtcg gggtccatct
     9421 tcgaggtcag cacgcccagg tcgaggccat tgatggtgac atcgccgtcg atctgcatct
     9481 tgccgctggc gagcgccttg cgggcagcca cttcaccgac gatcaacgcc atcagggtca
     9541 gcagcagcga gatcgcgccg gcccacaccg gggcccacag gaagccgccc ttgtgctccc
     9601 agaactcgcg cttgagcaac aacttgaact tgtgcgtggc gtgcggtgcc gggcgcggcg
     9661 tgcgcggggt cgcgacatcc atttccatcg cgtcgacggg ggtgttcatg cgtaggttcc
     9721 cttcatggtg gcgacgaaca ggtcggccag gcccggcgtg cgggtctcgc ccagcgcggc
     9781 cagccgcgcg gggtcggtgc cgtcgaacaa catcacggtc ttgccgaagg gcagggcgcg
     9841 ctcgtcgatc ggcttgagcg cacgcgcctc atcctgcttg ccggcatcga ccagcacttc
     9901 ggtgtagcgg acggcgaggg tgtccatcgg tgcatccagc accaccttgc cgtcgcggat
     9961 gaacatgacg tcggtgagga tgtgctcgat ctcctcgacc tggtgggtgg tgacgatgat
    10021 cgtcttgtcc tcgtcgaagt agtcctccag caggcgctgg tagaactgct tgcggtagag
    10081 gatgtccagg cccagggtgg gctcgtccag caccagcagc ttggcgtcga tcgccatcac
    10141 cagcgccagg tgcagttgca c
//
LOCUS       DAOOCX010000085        10152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_6242_length_10152_cov_6.487868, whole genome shotgun sequence.
ACCESSION   DAOOCX010000085 DAOOCX010000000
VERSION     DAOOCX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6242_length_10152_cov_6.487868"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PK399_08650"
     CDS             complement(<1..701)
                     /locus_tag="PK399_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPW13227.1"
                     /translation="MPPRLLFGLSLGLLLAPFASVQAQEPVPKEYRVLLVGNSLTYTN
                     NLPALLRAVGASQGTPITTETYAAPGGTLSERWRDGKAAEALRTRNFDVVVLQEIGGQ
                     VGCLASASRTRTAPCAATMQAHEAFAALARERGAKTLLFTTWSHDERMQGRINGGMRK
                     LAKENDATVFNAAGAIASLHKAQPDSTPYPDGTHPSTQASLMMAVALYRDITGNAPAP
                     KALRMNAPLLPVNAAV"
     gene            complement(782..1186)
                     /locus_tag="PK399_08655"
     CDS             complement(782..1186)
                     /locus_tag="PK399_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPW13228.1"
                     /translation="MAHADRRTPRRVRGDAVEDAALAHLQARGLRLLSRNATAHGGEI
                     DLVMLDGNGRDGAAVVFVEVRYRASSTFGGGAASVDAGKRRRLVRAAQAFLQQHPDHA
                     DAPCRFDVVAASGDPSNPALEWLQDAFRADDA"
     gene            complement(1173..2495)
                     /locus_tag="PK399_08660"
     CDS             complement(1173..2495)
                     /locus_tag="PK399_08660"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HPW13229.1"
                     /translation="MALKHTAIAVLWLAALAGCASVDVQQPAATPSAYSAAFMEAQAL
                     AQGHAALSGPARTANASRIESLLAGLDDAALARDAARLPAGDPLYSFAGRALLNRGLP
                     LPRPFDRGDVSFGAADRPAADRDGYRPPLKVGVLLPLSGGQSAAAASVRDGFLAGYYG
                     ERRRRPDVRFYDTAAGPSAAYARAAAEGNDFVVGPLGRDEVDAVFADDTPAVPLLALN
                     RGSRTPPAGSAAFSLSPEDEGISLAQYLIQRGAKRVLIQTGGEDAQRRTASTAKAHLQ
                     RRGLQVTEDLAAAQEVDALLLAVKGTQARALMPQLALAGITPGMRVATSQIISGTGKP
                     AEDAALDGIVYPTEAWAVRNVDGLPSQASAAARVDTAKGPAARLFAFGYDAWLLTGYL
                     ERLALSNNGDVAGATGRLSLDGFGNVLRVPGWSRFAGGVPVPLADGAR"
     gene            2557..3408
                     /gene="rsmI"
                     /locus_tag="PK399_08665"
     CDS             2557..3408
                     /gene="rsmI"
                     /locus_tag="PK399_08665"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPW13230.1"
                     /translation="MQRSGTLFVVATPIGNLGDFSARGREVLAQVAAICAEDTRRSGQ
                     LLAHFGIATPLLALHEHNEDAIAQKIVARLLAGDSLALVSDAGTPLVSDPGFRLVRAA
                     REAGIQVSPVPGASALVAALSVAGLPSDRFIFEGFLPAKPKARREHLQRLAGEPRTLI
                     FYESSHRIEEALDDAVAAFGADRPAVMARELTKLFETVLDGTLAQLAERVRADDNQRK
                     GEFVLMVHGAGEDADAKVVEGRRIYSTLAKHLPPSTAAKLASELSGAPRKSLYGGESS
                     EAADPAP"
     gene            complement(3525..4031)
                     /gene="rnpB"
                     /locus_tag="PK399_08670"
     ncRNA           complement(3525..4031)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK399_08670"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     assembly_gap    3643..3742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4059..4820)
                     /locus_tag="PK399_08675"
     CDS             complement(4059..4820)
                     /locus_tag="PK399_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HPW13231.1"
                     /translation="MCLVAFAWKSHPRWRLVLAGNRDEFHGRPTAALAAWPGSGILAG
                     RDLRSGGTWAGLGRDGRLAVITNVRDGLPPPFNGPSRGALPVDFLAGDRSASAHAAGL
                     AGIASDYAPFNLVVADADDCAFVGNHPASGAHHVTPGVHGLSNGAFDAPWPKTRRLRE
                     ALQAWIEAGHDDLDPLWQALTDERLAADTELPDTGIGPDLERRLSAAFIRGRDYGTRA
                     STLILVGHDGRARIHERRFGPDGVVAGETVLDSGD"
     gene            5152..5598
                     /gene="mraZ"
                     /locus_tag="PK399_08680"
     CDS             5152..5598
                     /gene="mraZ"
                     /locus_tag="PK399_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPW13232.1"
                     /translation="MFQGETAITIDDKGRLAIPTSYRDLVARECGNRLVVTYNPFEAG
                     CLYLYPLPVWEKVRDQVNALPRTKKNSRLLQLKLVGAAAFAEPDGSGRISLPASQRNA
                     VGIEKKAVLLGMGDKFELWSEQAHHEQIRQTLGDADLGDDLQDLVL"
     gene            5595..6542
                     /gene="rsmH"
                     /locus_tag="PK399_08685"
     CDS             5595..6542
                     /gene="rsmH"
                     /locus_tag="PK399_08685"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPW13233.1"
                     /translation="MSTGGAAIASAHLPVMFAEVMDGLAVKVDGTYLDGTFGRGGHAR
                     GVLQRLGPGGRLLVMDKDPEAIEVARQLASDDARVSWHRGSFADLAGWSATGDGLDGV
                     LFDLGVSSPQLDVAERGFSFGKDGPLDMRMDPESGESAAEWLARADEKEIADVLWTWG
                     EEKQSRRIARAIVQDRATKPFTRTAELAGLIARLMPRNDKKVHPATRTFQAIRIFINR
                     ELLDLELGLDAAMARLKPGGRLAVISFHSLEDRIVKQFMLRHAKAPPANRRMPVEVEF
                     TPSLRLVDGAQKAGEAELAANPRARSAVLRVAERIGVAA"
     gene            6539..6805
                     /gene="ftsL"
                     /locus_tag="PK399_08690"
     CDS             6539..6805
                     /gene="ftsL"
                     /locus_tag="PK399_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HPW13234.1"
                     /translation="MNAKLLLGALVLANVVTALLVVRDRHEHRQAFVALSKLERARDE
                     LNIEFGRLQLEQATWAESNRVDQVARSRLGMVFPRTEDIVVLRR"
     gene            6934..8679
                     /locus_tag="PK399_08695"
     CDS             6934..8679
                     /locus_tag="PK399_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPW13235.1"
                     /translation="MVCGTLGLCSVALIARALDLQVIDHAFYQQQGDARFVREIPIAT
                     SRGMITDRNGEPLAISSPVESIWGNPKELLKSPARLPALARALGMPQDELASRLSQRA
                     GKEFVYLKRRINPDEAARILALDIPGVSSQREYRRFYPQGEAVAHVLGFTNIDDRGQE
                     GLELAFDDWLRGTPGAKRVIRDRRGQIVESVDLVRAAQPGRDLALSIDRRIQYLAHRE
                     LRNAINLTGASSGSAVVLDVATGEVLAMVNFPTFNPNAVGTSPRDAHRNRAVTDLMEP
                     GSTMKPLTVAAGLEAGVITTTSLFNTSPGWIPNGKYRTTDTHNYGVLDTTGVIRKSSN
                     VGASLIAKRLGDAQFDAFLRRFGYGHVTGSGFPGEAAGLFPNHQRWSGTSKQTISYGY
                     GLNATPLQIATAYAALGNGGLLHQPSFVKGQHGTPKQVLDPVIANQVLHMMQTVTEPG
                     GTATQAAILGYHVAGKTGTSRKASAGGYSRRYIGYFAGLVPVEKPRFAMVVAVNDPTK
                     GSYYGGIVSGPVFRNVMEGALRMMDVSPDNLDAWLAAQAAAEAKRAKAAGHPVPVTAI
                     ADAGTTPAPAAGALQ"
     gene            8676..>10152
                     /locus_tag="PK399_08700"
     CDS             8676..>10152
                     /locus_tag="PK399_08700"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPW13236.1"
                     /translation="MTRAMLLSELLPELEGLSADLAVTGLVMDSREVKPGDAFVAIAG
                     FGAHGLNFVDAAREAGAAAILFEPPAPEALPAPADAIPVAGLRSRMGAMADSFHDHPS
                     AAMTTVGVTGTNGKTSTVQLLAQAWTLRGQKVGTVGTLGAGIWPKIVPTGFTTPLVLR
                     MHALLAELRDEGAQAVAMEVSSHALDQGRVDGVHFDVGVFTNLTRDHLDYHGDMASYG
                     AAKARLFDWPGLRAAAVNLDDAFGRELFEAVGGKVRAVGFSSRGAAGASVRADDVQLD
                     GSGIRFMLDAGGQAHPVRSPLLGRFNVDNLLGVATTLFALGMAPALVAETLSQLAPVD
                     GRMNRLGGEAGLPLVVIDYAHTPDALEQALASLRAHTAGALTCVFGCGGERDRGKRPQ
                     MAAIAERLADRVIVTDDNPRNEDGDVIVADIVEGFADAARVLVQRDRAAAIAQALATA
                     GPDDVVLVAGKGHEPYQEIAGVRHAFDDTAVARDLLRRRAQE"
BASE COUNT         1525 a         3425 c         3604 g         1498 t
ORIGIN      
        1 accgccgcgt tcaccggaag cagcggggcg ttcatccgca gcgccttcgg cgctggcgcg
       61 ttgccggtga tgtcgcgata cagggccacc gccatcatca gcgacgcctg cgtgctcggg
      121 tgggtcccgt ccggataagg cgtggagtcc ggttgtgcct tgtgcaggga tgcgatggca
      181 cccgcggcgt tgaacaccgt ggcgtcgttc tccttggcga gcttgcgcat gccgccattg
      241 atccggccct gcatgcgttc gtcgtgcgac caggtggtga acagcagggt ctttgcccca
      301 cgttccctgg ccagcgcggc gaatgcctcg tgtgcctgca tggttgcggc acagggcgcg
      361 gtgcgcgtgc gcgacgcgct ggccaggcag ccgacctggc cgccgatctc ctgcagcacc
      421 accacgtcga agttgcgcgt gcgcagcgcc tcggcggcct tcccgtcacg ccagcgctcg
      481 gacagcgtgc cgcccggcgc ggcgtaggtc tcggtggtga tcggcgtgcc ttgcgaggcg
      541 ccgactgcgc gcaacagcgc gggcaggttg ttggtatagg tcaggctgtt gccgaccagc
      601 agcacgcggt attccttcgg caccggctcc tgcgcctgca cgctggcgaa cggggcaagc
      661 aggaggccga ggctgaggcc gaacaggagg cgtggcggca tggtggcgtt cgcgagagga
      721 gacggggcag ggtacgggtt cgcacgccgc gccgcgtatg ccgttggttg gccggcgcgc
      781 ctcaggcgtc gtcggcgcgg aaagcgtcct gcaaccactc cagtgcgggg ttggagggat
      841 cgccactggc ggcgaccacg tcgaagcggc agggcgcgtc ggcgtggtcg ggatgctgtt
      901 gcaggaacgc ctgtgcagct cggaccaggc gccgccgctt gccggcatcc acggacgctg
      961 ctccgccgcc gaaggtgctg ctggcgcggt agcgcacctc gacgaacacc acggcagcgc
     1021 catcgcgccc gttgccgtcg agcatcacca ggtcgatctc gccgccgtgg gcggtggcgt
     1081 tgcgggacag caggcgcagg ccccgggcct gcaggtgggc gagtgcggcg tcttcgaccg
     1141 cgtcgccacg gacgcggcgc ggggtgcgcc ggtcagcgtg cgccatcggc cagcggcacc
     1201 ggcacgccgc cggcgaaccg cgaccagccg gggacgcgga gcacgttgcc gaacccgtcc
     1261 aggctcaagc gcccggtcgc gccggccacg tcaccgttgt tcgacagcgc cagccgttcc
     1321 agataaccgg tcagcagcca ggcgtcgtag ccgaaggcga acagccgcgc agcgggaccc
     1381 ttggcggtat ccacccgggc cgcggcgctt gcctgcgagg gcaggccgtc gacattgcgc
     1441 acggcccagg cctcggtcgg gtagacgatg ccgtccaggg cggcatcctc ggccggcttg
     1501 ccggtaccgg agatgatctg cgacgtcgcc acccgcatcc cgggcgtgat gcccgccagc
     1561 gcaagctgcg gcatcaacgc gcgcgcctgc gtgcccttca ccgccagcag cagcgcatcc
     1621 acctcctgcg ccgccgcgag gtcctcggtg acctgcagtc cgcggcgctg caaatgcgcc
     1681 ttggcggtgc tggcggtgcg ccgttgcgca tcttcgccac cggtctggat caacacgcgc
     1741 ttggccccgc gctggatcag gtactgggcc aggctgatgc cctcgtcctc cggcgacagc
     1801 gagaaggctg cgctaccagc gggcggcgtg cggctgccgc ggttcagcgc gagcagcggc
     1861 accgcagggg tgtcgtcggc gaagaccgcg tcgacctcgt cgcggccgag cgggccgacc
     1921 acgaaatcgt tgccctcggc ggcagcccgc gcgtaggcgg cgctgggtcc tgcggcggtg
     1981 tcgtagaaac gtacgtcggg gcggcgtcga cgctcaccgt agtagccggc caggaagcca
     2041 tcgcgcacgg acgccgcggc tgcggactgc ccgccactca gcggcagcag gacaccgacc
     2101 ttcagcggcg ggcggtagcc gtcgcggtcc gccgccggcc ggtcggccgc gccaaagctg
     2161 acatcgccgc gatcgaacgg ccgcggcagc ggcagcccgc ggttaagcag ggcgcggccg
     2221 gcgaaggaat acagcgggtc gccggccggc aggcgcgcgg cgtcgcgggc cagcgcggca
     2281 tcgtccaggc ccgccagcag ggattcgatg cggctcgcgt tggcggtgcg cgcaggcccc
     2341 gacaacgcgg catggccctg ggccagcgcc tgcgcctcca tgaaggcggc actgtaggcg
     2401 gagggcgtgg cggcgggctg ctgcacgtcc acgctggcgc agccggccag ggcggccagc
     2461 cacaggacgg cgatggcggt gtgtttcagg gccacgtatc ggcgctcgtt cgggaaccgg
     2521 ctaggattct acccgcgacc acgctcaagg caggcgatgc aacgttccgg aaccctgttc
     2581 gtggtggcca cgccgatcgg caacctgggc gatttcagcg cacgtgggcg cgaggtcctg
     2641 gcccaggtcg ccgcgatctg cgccgaggac acccgccgca gcggccagtt gctggcccat
     2701 ttcggcatcg ccacgccgct gctcgcgttg catgaacaca acgaggacgc gatcgcacag
     2761 aagatcgtgg cccgcctgct ggccggcgac tcgctggcgc tggtgtccga tgccggcacc
     2821 ccgctggtca gcgatcccgg cttccggctg gtgcgtgccg cccgcgaggc cggcatccag
     2881 gtcagcccgg tgcccggtgc cagcgcgctg gtggcggcgc tgagcgtggc cggcctgccc
     2941 agcgaccggt tcatcttcga aggcttcctg ccggccaagc ccaaggcccg tcgcgagcac
     3001 ctgcaacgcc tggccggcga accgcggacc ttgatcttct acgagtcctc gcaccgcatc
     3061 gaagaagcgc tggacgacgc cgtggcggcc ttcggcgcgg accggcccgc cgtcatggcg
     3121 cgcgaactga ccaagctgtt cgagaccgtc ctcgacggca ccctggcgca actggccgaa
     3181 cgcgtgcgcg ccgacgacaa ccagcgcaag ggcgaattcg tcctgatggt gcacggcgcc
     3241 ggcgaggacg ccgatgcgaa ggttgtcgaa gggcgtcgca tttactcgac cttggccaag
     3301 cacctgccgc cctcgaccgc cgccaagctg gcgtccgaac tgtcgggcgc gccgcggaaa
     3361 tcgctctacg gcggcgagtc aagcgaagca gcggatccag ccccctgagt cagggggcga
     3421 ttcgcgccga aggcgcgaat gggggtgtgg aagcgtggcc tggggcccaa agttcgcgca
     3481 gcgaactttg ggagcttcat cgcgccgtcg cgcgatgaag gtagcggcgg agccggccga
     3541 taagccgggt tctgtcgtgg acagtcattc ctctaggcgc atcgtcaccg atacgctcaa
     3601 gcaacctacc cggagacacc gcgggccgcg gcattgcctc ccnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnccgatacg ctcaagcaac ctacccggag acaccgcggg
     3781 ccgcggcatt gcctccctat ttggtcttgc tcccggtggg gtttgccgtg ccggcctgtt
     3841 gccaggctcg cggtgcgctc ttaccgcacc atttcaccct tgcctgccag attgctccga
     3901 cataggcggt atctttctgt tgcactttcc gtcggctcgc gccgcccagg cgttacctgg
     3961 caccgtgccc tgtggagccc ggactttcct cggcatcgat tgctcgatga cgcgactgcc
     4021 tggccgactc cgccgccggc attgtcgcac gtgtgacttc agtcgccgct gtccagcacg
     4081 gtttcgcccg cgaccacgcc atccgggccg aagcggcgct cgtggatgcg ggcccggcca
     4141 tcgtggccga ccaggatgag cgtgctggcg cgcgtgccgt agtcgcggcc gcggatgaag
     4201 gcggccgaca gccggcgttc caggtccggg ccgatgccgg tgtcgggcag ctcggtatcg
     4261 gcggcgaggc gctcatcggt cagtgcctgc cagagcgggt cgaggtcgtc atggccggcc
     4321 tcgatccatg cctgcagggc ttcgcgcagc cggcgtgtct tcggccaggg cgcgtcgaaa
     4381 gcgccgttgg acaggccgtg tacgcccggc gtgacgtggt gcgcgccgct ggcggggtgg
     4441 ttgccgacga aggcgcagtc gtcggcgtcg gccacgacca ggttgaacgg ggcgtagtcg
     4501 ctggcgatcc cggccaatcc ggcggcatgg gccgatgcgg atcggtcgcc ggccaggaag
     4561 tcgaccggaa gggcgccgcg cgagggcccg ttgaagggag gcggcaagcc gtcgcggaca
     4621 ttggtgatca ccgcgaggcg tccgtcgcgg cccaggcctg cccaggtacc gccggagcgc
     4681 aggtcgcgcc cggccaggat cccggagccg ggccaggcag ccagcgctgc ggtcgggcgg
     4741 ccatggaatt catcgcgatt gccggccagc acgagtcgcc agcgggggtg ggatttccag
     4801 gcgaaggcga ccaggcacat gccgcgcatt ctcgcagggg gctggggtct tcacgtcggg
     4861 gatttttgcg tcgcaacatg ggctggtttg gatctgaatg gaggccccgc gcgatgtgcc
     4921 gaatgtcacg gattctcaaa aattgagacg aaacagtggc ttgtggataa agcttgaaca
     4981 agtctctgaa gattgctgca agtccttgaa cggacaggtg aaaagtcctg tggattgttg
     5041 ttgacaaggg cataggccct gcctaatgtg gcgactcgtg ggaaaaccgg gaaattcgtg
     5101 gttttctcgt catgaactcc gccttagacg gaggcgacgc agggtagggg catgttccag
     5161 ggcgagaccg ccatcaccat cgacgacaag ggccggctgg cgatcccgac cagctaccgg
     5221 gaccttgtcg cgcgcgagtg cggcaatcgc ctggtcgtca cctacaaccc gttcgaggcc
     5281 ggctgcctgt acctgtaccc gctgccggtc tgggaaaagg tccgcgacca ggtcaatgcc
     5341 ctgccgcgga ccaagaagaa cagtcgcctg ctgcagctga agctggtcgg cgcggcggcc
     5401 ttcgccgaac ccgacggcag cggccgcatc tccctcccgg ccagccagcg caacgcggtg
     5461 ggcatcgaga agaaggcggt gctgctgggc atgggcgaca agttcgagtt gtggagcgaa
     5521 caggcccacc acgagcagat ccggcagacg ctgggcgatg ccgacctggg cgacgacttg
     5581 caggacctgg tgttgtgagt acgggtggcg cggccatcgc gtccgcccac ctccccgtga
     5641 tgttcgccga ggtcatggac ggtctggcgg tgaaggtgga tggaacctat ctggatggca
     5701 cgttcgggcg cggcgggcat gcgcgcggcg tgttgcagcg actcgggccg ggaggccgcc
     5761 tgctggtgat ggacaaggac cccgaggcga tcgaagtggc caggcaattg gccagcgacg
     5821 atgcgcgcgt gtcctggcat cgcggcagct tcgccgacct ggccggctgg tcggcgaccg
     5881 gcgacgggct ggacggtgtg ctgttcgacc tgggcgtgtc gtcgccgcag ctggacgtgg
     5941 ccgagcgcgg tttttcgttc ggcaaggacg gcccgctgga catgcgcatg gatcccgagt
     6001 cgggcgagag cgccgccgag tggctggccc gcgccgacga aaaagagatc gccgacgtgt
     6061 tgtggacctg gggcgaggaa aagcagtcgc gccggatcgc ccgcgccatc gtgcaggacc
     6121 gggcgacgaa gccgttcacc cgtaccgcgg agctggcggg actgatcgcg cggctgatgc
     6181 cgcgcaacga caagaaagtg catccggcga cgcgcacctt ccaggcgatc cgcatcttca
     6241 tcaatcgcga gctgctcgac ctcgagctcg ggctggatgc ggcgatggcg cggctcaagc
     6301 ccggcggccg gctggcggtg atcagcttcc attcgctgga agaccgcatc gtcaagcaat
     6361 tcatgctccg ccatgccaag gcgccgccgg ccaaccggcg gatgccggtc gaggtcgaat
     6421 tcacgccttc gctgcgcctg gtcgatggcg cgcagaaggc cggcgaggcc gaactggccg
     6481 ccaacccgcg cgcgcgcagc gcggtgctgc gcgtcgccga gcgcatcggg gtggcggcat
     6541 gaacgccaaa ctgctgctgg gcgcgctggt cctggccaac gtggtgaccg cgttgctggt
     6601 cgtgcgcgac cgccatgagc atcgccaggc cttcgtggcg ctgagcaagc tggagcgcgc
     6661 gcgcgacgag ctcaacatcg agttcggtcg gttgcagctg gagcaggcca cctgggcgga
     6721 gagcaaccgc gtcgaccagg tcgcgcgttc gcgcctgggc atggtgttcc cgcgcaccga
     6781 agacatcgtg gtgctgcgtc gatgaaactg ttcggcaagc acctgccatc gcccgggcgc
     6841 caggatggcg agcgtcgcga gcgcgcacga cccgccgcgg gcaaccggcc ccgcgcgcaa
     6901 cgcaacgggt tcgacctgcg cagccggttg ttgatggtct gcggcacgct gggcctgtgc
     6961 tcggtggcgc tgatcgctcg cgcgctggac ctgcaggtca tcgaccacgc gttctaccag
     7021 cagcagggcg atgcccgttt cgtccgcgaa atcccgatcg cgacctcgcg cgggatgatc
     7081 accgaccgca acggcgagcc gctggcgatc tcctcgccgg tggagtcgat ctggggcaac
     7141 ccgaaggaat tgctgaaatc gccggcgcgg ttgccggcgc ttgcgcgcgc gctgggcatg
     7201 ccccaggacg aactggcgag caggttgagc cagcgcgcag gcaaggaatt cgtgtacctg
     7261 aagcggcgga tcaacccgga cgaggcggcg cgcatcctgg cgctggacat ccccggcgtg
     7321 tccagccagc gcgaataccg ccgcttctac ccgcaaggcg aggcggtcgc ccacgtcctc
     7381 ggcttcacca acatcgacga ccgcggccag gaaggcctgg aactggcgtt cgacgactgg
     7441 ctgcgcggca ccccgggcgc caagcgcgtg atccgcgacc gccgcgggca gatcgtggag
     7501 agcgtggacc tggtccgtgc ggcgcagccg ggccgcgacc tggcgctgag catcgaccgt
     7561 cgcatccagt acctggcgca tcgcgagtta cgcaatgcga tcaacctcac cggtgccagc
     7621 agcggctcgg cggtggtgct ggacgtcgcc accggcgaag tgttggcgat ggtcaatttc
     7681 ccgaccttca atccgaatgc ggtgggcacc agcccccgcg acgcccaccg caaccgcgcc
     7741 gtgaccgacc tgatggagcc gggctcgacg atgaagccgt tgaccgtggc ggccgggctg
     7801 gaagcgggcg tcatcaccac gacctcgctg ttcaacacca gccccgggtg gatcccgaac
     7861 ggcaagtacc gcaccaccga tacccacaac tacggggtgc tggacaccac cggggtgatc
     7921 cgcaagagct cgaacgtcgg cgcatcgctg atcgccaagc gactgggcga tgcgcagttc
     7981 gatgccttcc tgcgccgctt cggctacggc catgtcaccg gcagcggctt cccgggcgag
     8041 gctgccggcc tgttcccgaa tcaccaacgc tggagcggca ccagcaagca gaccatcagc
     8101 tatggctacg gcctgaacgc cacgccgctg cagatcgcca ccgcttatgc ggcgctgggc
     8161 aatggcgggt tgttgcacca gccgagcttc gtcaagggcc agcacggcac gccgaagcag
     8221 gtgctggatc cggtcatcgc caaccaggtg ctgcacatga tgcagaccgt gaccgaaccg
     8281 ggcggcaccg cgacccaggc cgcgatcctc ggctaccacg tggccggcaa gaccggcacc
     8341 tcgcgcaagg ccagcgccgg cggctactcg cgtcgctaca tcggctattt cgccggcctg
     8401 gtgccggtgg agaagccgcg cttcgcgatg gtcgtcgcgg tgaacgatcc caccaagggg
     8461 tcctactacg gcggcatcgt ctccggcccc gtgttccgca acgtgatgga aggcgcgttg
     8521 cggatgatgg acgtgtcgcc ggacaacctg gatgcctggc tggccgcgca ggccgcggct
     8581 gaagccaagc gtgccaaggc cgccggccat cccgtgccgg tcacggcgat tgccgatgcc
     8641 ggaaccacgc ccgcacccgc cgcaggggcg ctgcaatgac ccgcgccatg ctgctcagcg
     8701 aactgctgcc ggaactcgaa ggcctgtcgg ccgatcttgc cgtcaccggc ctggtgatgg
     8761 acagccgcga ggtaaagccg ggcgatgcct tcgtcgcgat cgccggcttc ggtgcgcatg
     8821 gcctgaattt cgtcgatgcc gcgcgcgaag ccggtgcggc cgcgatcctg ttcgaaccgc
     8881 cggcgcccga ggccttgccg gcacccgccg atgcgatccc ggtcgccggc ctgcgctcgc
     8941 gcatgggcgc gatggccgac agcttccacg accatcccag cgcggcgatg accacggtcg
     9001 gggtgaccgg caccaacggc aagacctcga ccgtgcaact gctggcccag gcctggacgt
     9061 tgcgcgggca gaaggtgggc acggtgggca cgctcggcgc cggcatctgg ccgaagatcg
     9121 tgccgaccgg tttcaccacg ccgctggtgc tgcgcatgca tgcgctgctc gccgaactgc
     9181 gcgacgaagg cgcgcaggcg gtggcgatgg aagtctcctc gcacgcactc gaccaggggc
     9241 gcgtggatgg cgtgcatttc gacgtaggcg tgttcaccaa cctcacccgc gaccatctcg
     9301 actaccacgg cgacatggcc agctacggcg cggccaaggc gcggctgttc gactggccgg
     9361 gcctgcgcgc cgcggcggtg aacctggacg atgcgttcgg ccgcgagttg ttcgaggcgg
     9421 tcggcggcaa ggtgcgcgcg gtcggctttt cctcgcgcgg cgccgccggc gccagcgtgc
     9481 gcgccgacga cgtgcagctg gacggcagcg gcatccgctt catgctggac gccggtggcc
     9541 aggcgcatcc ggtgcgttcg ccgctgctcg gccgcttcaa cgtcgacaac ctgctgggcg
     9601 tggccacgac cctgttcgcg ctgggcatgg cgccggcgct ggtcgccgaa accctgtcgc
     9661 aactcgcgcc ggtcgacggc cggatgaacc gcctcggtgg cgaggccggc ctgccgctgg
     9721 tggtgatcga ttacgcgcac acgccggacg cgctggagca ggcactggcc tcgctgcgcg
     9781 cgcataccgc aggcgcgctg acctgcgtgt tcggctgcgg cggcgagcgc gatcgcggca
     9841 agcgcccgca gatggcggcg atcgccgaac ggctggccga ccgcgtcatc gtcaccgacg
     9901 acaacccgcg caacgaagat ggcgacgtca tcgtcgccga catcgtcgaa ggcttcgccg
     9961 atgccgcgcg cgtgctggtg cagcgcgacc gcgccgccgc catcgcacag gcgttggcca
    10021 ccgcgggccc ggacgatgta gtgctggtgg ccggcaaggg ccacgagccg taccaggaaa
    10081 tcgccggcgt ccgccatgcg ttcgacgaca ctgcggtcgc ccgcgatttg ttgcgtcgtc
    10141 gcgcgcagga gt
//
LOCUS       DAOOCX010000086        10079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_6319_length_10079_cov_9.004489, whole genome shotgun sequence.
ACCESSION   DAOOCX010000086 DAOOCX010000000
VERSION     DAOOCX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10079
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6319_length_10079_cov_9.004489"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /gene="speE"
                     /locus_tag="PK399_08705"
     CDS             <1..716
                     /gene="speE"
                     /locus_tag="PK399_08705"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPW13237.1"
                     /translation="MLIDGAMMLTTRDNFLYHEMMSHPALFTHADPKRVVIIGGGDCG
                     TLREVLRHPGVQRATQCDIDEQVTRMAEKYFPELCESNNDPRAELLFDDGIAYMANCE
                     PGSVDIVIVDSTDPVGPAEGLFNAAFFASCHRALKDDGILVQQSESPLVLLDLIKEMR
                     VEMGKAGFASFQTLPFPQPCYPTGWWSCTMAKKAADADFGFREADARGKGFRTAYYSA
                     DLHKGAQVLPPFVAEALAG"
     gene            823..2685
                     /locus_tag="PK399_08710"
     CDS             823..2685
                     /locus_tag="PK399_08710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW13238.1"
                     /translation="MGWLAALAMPCLLLAQPAPSPATTAVFDSILQRVIGPDSLSASS
                     TEYDAELERLRAALPAGDPARDARFRSVYCGSSTWRDPVQGLAYAESALALARDLNDV
                     ASQARALLCRAEYVMLTSGTQRGLPDVDKALDLLGNGREPQLLAETLETRGDILSLLG
                     EQAKAMVDFQRARAAYRQAGIAGEVESLVLSIAVAYRRMGDADQARRYFTQALQRMQA
                     RADWEGVATNMIQLGFLQEEAGAPERAMRPFQQALDVAIAHQLPDTANSALLGLAEAQ
                     INLGQGEAGLRTLERAREGFASREDASSEDSLQLLTGQALARQGRHAEALAHYRQALP
                     LIKRNGNDRYLAMLYKAQSATLEAMGHPAAALSDYKRYADLQLTLQRKMRLEQGRLLE
                     YEDEIRRSEFENRRLRSEAAIKQQQVQALERVRHWQWMTIILGALLLALLTSLAWRHG
                     RTSRRLSRESLIDPLTGISNRAAIEAEAIRAIGGTTRPGASVSLLILDLDHFKAINDQ
                     HGHAAGDRVLRAAADAWHTVLRGRDPLGRIGGEEFVVVCADTSLEQAMVVAERLREAT
                     TALRFDDIDPALRVTVSIGVAQSQQSDDSHEDVLARADAALYRAKQRGRDRVEH"
     gene            complement(2709..3047)
                     /locus_tag="PK399_08715"
     CDS             complement(2709..3047)
                     /locus_tag="PK399_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPW13239.1"
                     /translation="MKMVMAVIKPFKLDDVREALADAGVAGITATEVKGFGRQKGHTE
                     LYRGAEYLVDFLPKIKLEVAVPDEQVDAVVEAIMRAAGTGKIGDGKIFVWDLGKVVRI
                     RTGELDADAI"
     gene            3219..3476
                     /locus_tag="PK399_08720"
     CDS             3219..3476
                     /locus_tag="PK399_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HPW13240.1"
                     /translation="MIDLAQLDELARRLSSLVPPGLRESREELQQNFKSVLQAGLGKL
                     DLVTREEFDVQRAVLLRTREKLDALEQRLAELADAPQPPRH"
     gene            3488..5017
                     /locus_tag="PK399_08725"
     CDS             3488..5017
                     /locus_tag="PK399_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPW13241.1"
                     /translation="MGLAIAHSRARVGVRAPEVRVEVHLAGGLPSMSIVGLPEAAVRE
                     AKDRVRAAIQCAQFEFPSRRITINLAPADLPKDGGRFDLAIALGILAASGQLSNDALQ
                     EWEFLGELALTGEIRSVDGVLGAAIAAGEAGRRLLVAPGNGPEAALASTVDVRTARTL
                     LEVCAALDGGKRLPRAEPLPCRNRPPPDLADVRGQAQARRALEIAAAGGHHVLFAGPP
                     GSGKTLLASRLCGILPPPSETEALEAAAIASARGFGEGSRGLDPTRWRQRPFRAPHHT
                     ASAVALVGGGADPRPGEISLAHHGVLFLDELPEWSRHALEVLREPLESGSVHISRAAR
                     HCEFPARFQLVAAMNPCPCGWAGDPSGRCLCNDEMIRRYRARISGPLLERIDLHVEVP
                     RLPPSALRTDGPPGESSASVGERVLRARTVQEARCGLLNARLDQARTDAHCRLAPRDT
                     ALLERAVDSLQLSARSLHRILRVARTIADLADSHDIETPHLTEAIGYRRLERGRERAA
                     A"
     gene            5085..7172
                     /locus_tag="PK399_08730"
     CDS             5085..7172
                     /locus_tag="PK399_08730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014509407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPW13242.1"
                     /translation="MSLHRTLVATAIATCLLAACDRAPGEPTAKAATPATAPASINDI
                     ELIPRDALFGNPERGNVQISPDGKTLSWIAAVDGVMNVWVAPAGDIAKARAVTADKAR
                     GIRQYFWSHHPDTLLYMRDTGGDENFHLFSVNLATGETRDLSNFPNTRAEMAGLSHLH
                     PGAVLVAMNDRDPKWHDLYRVDLATGQRTLVQKNTGEFAGYIADPDFKVRMATKSRAD
                     GGSDLLEPDGKGSWKIVGEIPFADSLTTFPGGYTSDGKTQYFFDSRGRNTTALYAIDV
                     ASGAKTLVHEDARADVAGAIDDPNTNKAQAVSVNYLRNEWKVIDPAIAADMEALKAIG
                     PGDFSISARTQDDKTWIVAYSAAETPAVYYRYVRGGKPEKLFSARPALDGKPLVAMWP
                     QEIKSRDGLTLVSYLSLPKTADANNDGKADRPVPMVLLVHGGPWARDDYGYSGYDQWL
                     ANRGYAVLAVNYRGSTGFGKDFVNKSDGEWAGKMHDDLLDAVDWAVKNGVTTKDDVAI
                     MGGSYGGYATLTGLTFTPDTFKCGVDIVGPSNLNTLLSTIPPYWAAFFEQFAKRVGDP
                     RTEAGRKLLEERSPLTHVAKITKPLLIGQGANDPRVKQPESDQIVKAMQEKNIPVTYV
                     LFPDEGHGFQRPENSKAFNAVAEGFLAQCLGGRAQPIGSDFAGSSISVPAGADGVPGL
                     VEALKSHTAAVKN"
     gene            complement(7194..8432)
                     /locus_tag="PK399_08735"
     CDS             complement(7194..8432)
                     /locus_tag="PK399_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptide maturation dehydrogenase"
                     /protein_id="HPW13243.1"
                     /translation="MLIRRCAVVWLEPREVASFQLDDLLAGGTGVVGRLAWFAHAPHL
                     GGAIEIEALHAPILGALGPVDWIERDVLDARFGTEAIEALLGVGLLLPDAEGDPWARA
                     DSAFRGQGWHPPAAIGHMAARWEGVDGPKAMVDADVDTNEGLLRQHGSPPPAMAPPIA
                     PETQVVLPRIERDDFDALLDTRATCRNYDPDAFVPLATFSQLMARVFGARGIGHPAPG
                     FDVYKRTSPSGGSLHPVDCYLVVQRVEGIVPGLYRYRIESHALEAVVPAVAPPQPGDA
                     GTHPLGDAAPRAWTPGELQAFSRIAVAGQDWFAEAPVLCVLAPRFHRNFWKYRNHAKA
                     YRVCVLDAGHLSQTLQLCATQAGLGPFVTGAINEVDLERAFGMTAYAQSPLAVCGFGK
                     RSATLTESEFDPNRRVWPRD"
     gene            8539..8781
                     /locus_tag="PK399_08740"
     CDS             8539..8781
                     /locus_tag="PK399_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP-related RiPP peptide"
                     /protein_id="HPW13244.1"
                     /translation="MAKLLLDKLETDNDFRKAFVDSPEQALRSIGHTEGTECLALKPG
                     ATLASPEQIKASRDKLEQSLVGIQSATCPLDAQEAM"
     gene            complement(8863..>10079)
                     /locus_tag="PK399_08745"
     CDS             complement(8863..>10079)
                     /locus_tag="PK399_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13245.1"
                     /translation="LAHADMAQLLAGFGEVGAAAGEWRKALGIPGRLSPQEKLRIELM
                     LLQHTPPADYFRKVDEYLALYPDDSQVISRSGTNLWHQRNDFRAAEARYRLATKPAYP
                     NQGSIQYSLGVMLLGQERYAEAEAAFKKSREWGFSGWSEFHARAYDVRGQHADADRIY
                     LQSSAGKAGWRGEEGVVTGIDRGDLAQANEMARAWHQQARDAGDRLESLRARAAIATL
                     DVMGNPKGSRTELRALIADSKLWAQEVDAVYPPTSAELQLYAGLLAAYTDDPGLVADA
                     LRGVEGSRAVRDYPTVAQLREVLLAEQERLSGKPQAAIARLAPLARQERALVAVHWAL
                     MNAQRATGNTEAASKQSRWLATHRGRVFVESTTTEVLQFFNVAVAVQARSDAAAVAPA
                     ASARPAPTAPAR"
BASE COUNT         1671 a         3583 c         3380 g         1445 t
ORIGIN      
        1 tcatgctgat cgacggcgcg atgatgctga ccacccgcga caacttcctc taccacgaga
       61 tgatgtcgca cccggcgctg ttcacccacg ccgacccgaa gcgcgtggtc atcatcggcg
      121 gcggcgactg cggcaccctg cgcgaggtgc tgcgccaccc gggcgtgcag cgcgcgaccc
      181 agtgcgacat cgacgaacag gtcacgcgca tggcggagaa gtacttccct gaactctgcg
      241 agtcgaacaa tgatccgcgc gccgaactgc tgttcgacga cggcatcgcc tacatggcga
      301 actgcgagcc cggcagcgtg gacatcgtga tcgtggattc caccgatccg gtcggcccgg
      361 ccgaaggcct gttcaacgcc gcgttcttcg ccagctgcca ccgtgcgctg aaggacgacg
      421 gcatcctggt gcagcagagc gaatcgccgc tggtgctgct cgacctgatc aaggaaatgc
      481 gggtggagat gggcaaggcc ggcttcgcca gtttccagac cctgccgttc ccgcagccct
      541 gctaccccac cggctggtgg agttgcacga tggcgaagaa ggccgccgac gccgatttcg
      601 gcttccgcga ggccgacgcg cgcggcaagg gcttcaggac ggcgtactac agcgccgacc
      661 tgcacaaggg ggcgcaagta ttgccgccgt tcgtcgccga ggcgctggcc ggctgacata
      721 ctgcgaacat cggcacggac gcgagcagca accggcatta gcctgccggg ttgctgcatg
      781 ggatggggaa cacgcgtggc cagttggcgg gatcgcggga ggatgggatg gctcgccgcc
      841 ttggcgatgc cgtgcctgct gctggcccag ccggccccgt ccccggccac gacggccgtc
      901 ttcgacagca tcctgcagcg ggtgatcggg cccgattcgc tgtctgcgag cagtaccgag
      961 tacgacgccg agctggagcg cctgcgcgct gcgttgccgg cgggcgaccc ggcacgcgac
     1021 gcgcgcttcc gttcggtcta ctgcggcagc tcgacctggc gcgatccggt ccagggcctg
     1081 gcctacgccg agtccgcgct cgcgttggcc cgcgatctca atgacgtagc ttcgcaggcg
     1141 cgcgccctgc tttgccgcgc cgaatacgtg atgctgacca gcggcaccca gcgcggactg
     1201 ccggacgtgg acaaggccct ggacctgctt ggcaacgggc gcgaaccgca attgctggcc
     1261 gaaacgctgg agacccgcgg cgacatcctg tccctcctcg gcgagcaggc caaggcgatg
     1321 gtcgacttcc agcgggcgcg cgccgcctac cggcaggccg gcatcgccgg cgaggtcgag
     1381 tcgctggtcc tcagcatcgc cgtggcctat cggcggatgg gcgatgccga ccaggcgcgc
     1441 cgctatttca cccaggcgct gcagcgcatg caggcgcgtg cggactggga gggcgtggcc
     1501 accaacatga tccagctggg tttcctgcag gaagaagccg gcgcgcccga acgtgccatg
     1561 cggccgttcc agcaggcgct ggacgtggcc atcgcgcacc agctgccgga caccgccaac
     1621 tcggcgctgc tcggcctggc cgaagcgcag atcaacctgg ggcagggcga ggccgggctg
     1681 cgcacgctgg aacgcgcgcg ggaagggttc gcgagcaggg aggacgcgtc ctcggaggac
     1741 agcctgcaac tgctgaccgg ccaggccctg gcccggcagg gccggcacgc agaagcgctg
     1801 gcgcactacc ggcaggcctt gccgctgatc aagcgcaatg gcaacgaccg ctacctggcc
     1861 atgctctaca aggcgcagtc cgccaccctg gaggcgatgg gacatcccgc cgccgcgttg
     1921 tcggactaca agcgctacgc cgacctgcag ctgaccctgc aacgcaagat gcgcctcgag
     1981 cagggtcgcc tgctggagta cgaggacgag atccgccgca gcgagttcga gaaccggcgc
     2041 ctgcgcagcg aagccgcgat caagcagcag caggtgcagg cgctggagcg cgtgcgccac
     2101 tggcaatgga tgaccatcat cctgggcgcg ctgctgttgg ccctgctcac ctcgctcgcc
     2161 tggcggcacg ggcgcacctc gcggcggctc agccgggaaa gcctgatcga cccgttgacc
     2221 gggatttcca accgcgccgc gatcgaggcc gaggcgatcc gcgcgatcgg cggaaccacc
     2281 cggcccggcg cgtcggtgtc gctgctgatc ctggacctcg accatttcaa ggcgatcaac
     2341 gaccagcatg gccatgctgc cggcgatcgc gtcctgcgcg ccgccgccga cgcctggcac
     2401 accgtgctgc gtggccgcga cccgctgggc cggatcggtg gcgaggagtt cgtggtggtt
     2461 tgcgcggaca ccagcctcga gcaggccatg gtcgtcgccg aacgcctgcg cgaggccacc
     2521 acggccctgc gcttcgacga catcgacccg gcgttgcggg tcacggtcag catcggcgtg
     2581 gcccagtcgc aacaaagcga cgacagccac gaagacgtgc tggcgcgcgc cgacgccgcg
     2641 ctctatcggg ccaagcagcg cggccgcgat cgcgtcgaac actgacctgc gggtgggacg
     2701 cccggcgcct agatggcgtc ggcgtccagt tcgccggtgc ggatccgcac caccttcccc
     2761 aagtcccaga cgaagatctt gccgtcgccg atcttgcccg tgccggcggc acgcatgatc
     2821 gcctcgacca cggcatcgac ctgctcatcc ggcaccgcga cctccagctt gatcttgggc
     2881 aggaagtcga ccaggtactc ggcaccgcga tacaactccg tgtggccctt ctggcggccg
     2941 aagcccttga cctcggtcgc ggtgatgccc gccacgccgg cgtccgccaa cgcctcgcgc
     3001 acgtcgtcca gcttgaatgg cttgatcacc gccatcacca ttttcatcac gacgcctccc
     3061 agtggtcggc gcgagcatac cgcgcggcgc cgatgcatac tccacggccg gacgcggggt
     3121 tccctgaccg cgccccgcac cctgcaccga cggccgtggc gcaccgcgat gcgcatgctg
     3181 gcgacacccg caggagaacc ccgatgacac ccgagcgcat gatcgacctg gcccaactcg
     3241 acgaactcgc ccgccgcctc agcagcctgg tcccaccggg cctgcgcgag agccgcgagg
     3301 aactgcagca gaacttcaag tcggtgctgc aggccggatt gggcaagctg gacctggtga
     3361 cccgcgagga attcgacgtg cagcgcgcgg tcctgctgcg cacccgcgag aaactcgacg
     3421 cgctcgaaca gcgcctggcc gaactggccg acgccccgca accgccccgc cactgagggc
     3481 cgccaccatg ggtcttgcga tcgcgcacag ccgtgcgcgg gtgggcgtgc gtgcgcccga
     3541 agtccgggtg gaagtgcacc tggccggtgg cttgccgtcg atgtcgatcg tgggactgcc
     3601 ggaagccgca gtgcgcgaag ccaaagaccg ggtgcgcgcg gcgatccagt gcgcgcagtt
     3661 cgaatttccg tcgcggcgca tcaccatcaa cctggcaccg gccgacctgc cgaaggacgg
     3721 cgggcgcttc gacctggcca tcgcactggg gatcctggcc gccagcgggc agttgtccaa
     3781 cgacgcgttg caggaatggg aattcctcgg cgagctcgcg ctgaccggcg aaatccgcag
     3841 cgtcgatggc gtactcggtg ccgccatcgc cgccggcgag gcgggccgac gcctgctggt
     3901 cgctcccggc aacgggcccg aggccgcgct ggcctcgacc gtggacgtgc gcaccgcccg
     3961 cacgttgctc gaggtctgcg ccgcgctgga tggcggcaag cggctgccgc gtgccgagcc
     4021 gctgccctgc cgcaaccggc caccaccgga cctggctgat gtgcgcgggc aggcgcaggc
     4081 ccggcgcgcg ttggagatcg ccgcggccgg gggccaccac gtgctgttcg ccggcccgcc
     4141 tggcagcggc aagaccctgc tggcctcgcg cttgtgcggg atcctgcccc cgcccagcga
     4201 gaccgaagcg ctggaagcgg cggcgatcgc ctccgcgcgc ggcttcggcg aaggcagccg
     4261 cggactggat cccacccgct ggcggcagcg cccgttccgc gcaccccacc acacggcgag
     4321 cgcggtggcg ctggtcggtg gcggcgccga tccgcgcccc ggcgagatct cgctggccca
     4381 ccacggcgtg ttgttcctgg acgaacttcc cgaatggagc cggcatgcgc tggaagtcct
     4441 gcgcgagccg ctggaatccg gcagcgtgca catctcgcgc gccgcgcgcc attgcgaatt
     4501 ccccgcccgc ttccagctgg tggcggcgat gaacccctgc ccctgcggct gggcggggga
     4561 tccctccggc cgctgcctgt gcaacgacga gatgatccgc cggtatcgcg cgcgcatctc
     4621 cgggccattg ctcgaacgca tcgacctgca cgtggaagtg ccgcgactgc cgcccagtgc
     4681 actgcgcacc gatggcccac cgggcgaatc gagtgcgtcg gtgggcgaac gggtgctgcg
     4741 ggcgcgcacc gtgcaggagg cacgctgcgg cttgctcaat gcgcgcctcg accaggcgcg
     4801 gaccgacgcg cactgccggc tcgccccgcg cgacaccgcc ctgctcgaac gcgcggtgga
     4861 cagcctgcaa ttgtccgcgc gctcgctgca ccggatcctg cgcgtcgccc gcaccatcgc
     4921 cgacctcgcc gacagccacg acatcgaaac gccccacctc accgaggcga tcggctaccg
     4981 ccggctggag cgcgggcggg agcgcgcggc ggcctgacct tcggcatgtc ttacgacggc
     5041 agcgtttccg tatgctccgg cgtgggatcc cacggagaga cgccatgtcg ctgcaccgca
     5101 cgctcgtcgc caccgccatc gccacctgcc tgcttgccgc ctgcgaccgc gcgccgggcg
     5161 agccgaccgc caaggccgcc acgccggcca ccgcaccggc atcgatcaac gacatcgaac
     5221 tgatcccgcg cgacgccctg ttcggcaacc ccgagcgcgg caacgtgcag atcagcccgg
     5281 acggcaagac gctgagctgg atcgccgccg tcgacggggt gatgaatgtc tgggtcgcgc
     5341 cggcaggcga catcgccaag gcacgcgcgg tgaccgccga caaggcgcgc ggcatccgcc
     5401 agtacttctg gagccatcac ccggacaccc tgctgtacat gcgcgatacc ggcggcgacg
     5461 agaacttcca cctgttctcg gtgaacctgg ccaccggcga aacgcgcgac ctgagcaact
     5521 tcccgaacac gcgcgcggaa atggccggcc tgagccacct gcatccgggc gcggtgctgg
     5581 tcgccatgaa cgaccgcgac ccgaagtggc acgacctcta ccgggtcgac ctggcgaccg
     5641 gccagcgcac cctggtgcag aagaacaccg gcgagttcgc cggctacatc gccgacccgg
     5701 acttcaaggt gcggatggcg accaagtcgc gcgcggacgg cggcagcgac ctgctggaac
     5761 cggatggcaa gggcagctgg aagatcgtcg gcgagatccc gttcgccgat tcgctgacca
     5821 cgttcccggg cggctatacc agcgacggca agacccagta tttcttcgac tcgcgcgggc
     5881 gcaacaccac cgcgctgtac gccatcgacg tcgccagcgg cgcgaagacc ctggtgcacg
     5941 aggacgcgcg cgcggacgtg gccggcgcca tcgacgaccc gaataccaac aaggcgcagg
     6001 cggtctcggt caactacctg cgcaacgagt ggaaggtcat cgatcccgcg atcgccgccg
     6061 acatggaggc gctgaaggcg atcggccccg gcgacttcag catcagcgca cgcacgcagg
     6121 acgacaagac ctggatcgtg gcctactccg ccgccgagac cccggcggtc tactaccgct
     6181 acgtgcgcgg cggcaagccg gaaaagctgt tctccgcgcg gccggcgctg gacggcaagc
     6241 ccctggtcgc gatgtggccg caggagatca agtcgcgcga tggcctgacc ctggtgagct
     6301 acctgagcct gccgaagacg gcagacgcga acaacgacgg caaggccgac aggccggtgc
     6361 cgatggtgct gctggtgcat ggcggcccgt gggcgcgcga cgactacggc tacagcggct
     6421 acgaccagtg gctggccaac cgcggctatg cggtgctggc ggtcaactac cgtggctcca
     6481 ccggcttcgg caaggacttc gtcaacaagt cggatggcga atgggccggc aagatgcatg
     6541 acgacttgct cgatgcagtc gactgggcgg tgaagaacgg cgtcaccacc aaggacgacg
     6601 tggcgatcat gggcggcagc tacggcggct acgccacgct caccggcctg accttcaccc
     6661 cggacacctt caagtgcggc gtcgacatcg tcggtccgtc caacctcaac acgttgttga
     6721 gcacgattcc gccctactgg gccgccttct tcgagcagtt cgccaagcgc gtcggcgacc
     6781 cgcgcacgga agcggggcgg aagctgcttg aggagcgctc cccgctgacc cacgtggcga
     6841 aaatcaccaa gccgctgctg atcggccagg gcgccaacga cccgcgggtg aagcagccgg
     6901 aaagcgacca gatcgtcaag gcgatgcagg agaagaacat cccggtgacc tacgtgctgt
     6961 tccccgacga aggccacggt ttccagcgac cggagaacag caaggcgttc aacgcggtgg
     7021 ccgaaggctt cctggcgcag tgcctgggcg gccgcgcgca gccgatcggc agcgacttcg
     7081 ccggctccag catcagcgtg ccggcaggcg cggacggcgt gcccggcctg gtggaggcgc
     7141 tgaagtcgca cactgcggcg gtgaagaact gaccggccgg ctggccgcgc ggctcagtcg
     7201 cgcggccaga ccctgcggtt gggatcgaac tccgactcgg tcagcgtggc cgagcgcttg
     7261 ccgaacccgc agacggccag cgggctttgc gcataggcgg tcatcccgaa cgcacgctcc
     7321 aggtcgacct cgttgatcgc gccggtgacg aacggtccca gccccgcctg ggtcgcgcac
     7381 agctgcaggg tctgcgacag gtggccggcg tccagtacgc agacgcgata ggccttggca
     7441 tggttgcggt acttccagaa attgcgatgg aagcgcggcg ccagcacgca cagcaccggc
     7501 gcctcggcga accagtcctg cccggccacc gcgatccgcg aaaacgcctg cagttcgcct
     7561 ggcgtccatg cgcgcggtgc ggcatcgccc agcggatggg tgccggcatc gccgggctgc
     7621 gggggagcca ccgcgggaac caccgcttcc agcgcatgtg actcgatgcg gtagcggtag
     7681 aggccgggca cgatgccctc cacgcgctgc acgaccaggt agcaatcgac gggatgcagc
     7741 gacccgcccg acgggctggt gcgcttgtag acatcgaacc ccggcgccgg gtggccgatg
     7801 ccgcgcgcgc cgaacacccg cgccatcagt tgtgagaagg tcgccagcgg cacgaacgcg
     7861 tcgggatcgt agttgcggca ggtcgcgcgg gtgtccagca gggcgtcgaa gtcgtcgcgt
     7921 tcgatccttg gcaggacgac ctgcgtttcc ggcgcaatgg gtggcgccat ggccgggggc
     7981 ggcgagccat gctggcgcaa caggccctca ttggtgtcga catcggcatc gaccatggcc
     8041 ttgggcccgt cgacgccttc ccagcgcgcg gccatgtgcc cgatcgcggc gggcggatgc
     8101 cagccttgcc cccggaacgc cgaatccgcg cgcgcccacg ggtcgccttc cgcatccgga
     8161 agcaacaggc ccacgccgag caaggcctcg atcgcctcgg tgccgaagcg tgcgtccagc
     8221 acgtcgcgct cgatccagtc caccggaccc agtgcaccga ggatcggcgc atggagcgcc
     8281 tcgatttcga tcgcgccacc gaggtgcggc gcatgcgcga accaggccag ccgccccacc
     8341 acgccggtgc caccggcaag caggtcgtcg agctggaaac tggcgacttc gcgcggttcc
     8401 agccagacca ctgcgcatcg cctgatcaac atgccgcact ccgccgcgtc cccatgcggt
     8461 ggtattgtcc gccttccatt caacaggggg aatgccatgg cacaagacaa cgggtcgccc
     8521 gccggattgc atccgcgcgt ggccaagctg ctgctggaca agctggagac ggacaacgat
     8581 ttccgcaaag ccttcgtgga ctcgcccgaa caggcgcttc gttcgatcgg ccacacggaa
     8641 ggcacggagt gcctcgcgct caagccgggc gcaaccctgg cgtcgccgga gcagatcaag
     8701 gcatcgcgcg acaagctgga gcagtcgctg gtcggcatcc agagcgccac ctgcccgctg
     8761 gatgcccagg aagccatgta accggttcga gcatccgagt tgcggcaacg gaggcccggt
     8821 ggctgccagc caccgggcct tttcgcatcc tggcggaacg ccttagcgcg cgggggcggt
     8881 gggtgctggc cgcgcggacg cggcaggcgc cacggccgca gcatccgaac gcgcctgcac
     8941 ggcaacggcg acattgaaga actgcaacac ttccgtggtc gttgattcga cgaatacacg
     9001 accgcgatgc gtcgcaagcc agcgcgattg cttcgatgca gcctctgtgt tgccagtggc
     9061 gcgctgggca ttcatcagcg cccaatgcac cgcgaccaac gcacgttcct gtcgcgccag
     9121 cggagccagg cgtgcgatgg cagcctgtgg tttgcctgag agcctttcct gctccgccaa
     9181 caacacctcc cgcaattgcg ccaccgtcgg gtagtcacgc acggcgcgac tgccttcgac
     9241 ccctcgcagc gcgtccgcga ccaaccctgg atcatccgtg taggcggcaa gcaagcctgc
     9301 atacaactgc agttccgcag aggtgggcgg gtagacggca tcgacttcct gggcccaaag
     9361 cttggagtcc gcaatcaggg cacgcaattc ggtgcggctc cccttgggat ttcccatcac
     9421 atccaaggtg gcgatggcag cgcgcgcgcg cagggactcg agccgatctc cagcatccct
     9481 ggcctgctga tgccaagcac gcgccatttc attggcctgc gccaagtcgc cgcgatcaat
     9541 cccggtgact acgccttcct cgccccgcca ccccgccttt ccggcggagc tttgcagata
     9601 gatgcgatcc gcatctgcat gttgaccacg cacgtcgtag gctcgggcgt ggaattcact
     9661 ccacccgctg aagccccatt cgcgcgactt tttgaatgcg gcctccgcct cggcgtaacg
     9721 ttcctggccc agcaacatga cgcccaagct gtactggatg ctgccctggt tcggatacgc
     9781 cggcttggtc gccaaccgat acctggcttc cgcggcacgg aaatcgttgc gctggtgcca
     9841 gaggttggtg cccgaacgcg agatgacctg gctgtcgtcg ggatacagcg ccaggtattc
     9901 gtcgaccttg cggaaataat cggcgggtgg cgtgtgctgg agcagcatca gttcgatgcg
     9961 caacttttcc tgcggggaca gccgcccggg gatcccgagc gctttccgcc attcgcccgc
    10021 ggcagcgccg acctctccga atccggccag caactgcgcc atgtccgcgt gcgccagcg
//
LOCUS       DAOOCX010000087        10076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_6322_length_10076_cov_9.704221, whole genome shotgun sequence.
ACCESSION   DAOOCX010000087 DAOOCX010000000
VERSION     DAOOCX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10076
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6322_length_10076_cov_9.704221"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PK399_08750"
     CDS             <1..747
                     /locus_tag="PK399_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HPW13246.1"
                     /translation="AGGGLVPWAVLSGYGTDTQDGGTAFATRVDTGDYSLDAAGVAYT
                     FRNRIELSYARQRFGLGELQRRLALPVGRFDAEVIGAKVRLGGDLVYTAMPQLSFGVQ
                     HKRQLDFAIPSAVGARSDSGTDVYLAASKLFLGAAFDRNLLFNATLRSTSANQAGLLG
                     FGGDRKAGRSLVLESSVALLLDPRWAVGVEYRQKPDNLGFAREDDWKDAFVAWFPNKR
                     VAVVAAYADLGSIATLDRQRGPYLSLQASF"
     gene            747..1181
                     /locus_tag="PK399_08755"
     CDS             747..1181
                     /locus_tag="PK399_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HPW13247.1"
                     /translation="MATHPFRLACLSVLLPAFLGACASQPRTTTYDQLGGAAGIEGIV
                     DALLEKIIEDERINFQFADTDIVRLRSMLIEQFCAESGGPCMYSGLSMQESHAGRNID
                     TAQFNALVEDLIDVMTARRIPVGAQNRLLRRLAPMHGEIVTP"
     gene            complement(1194..2144)
                     /locus_tag="PK399_08760"
     CDS             complement(1194..2144)
                     /locus_tag="PK399_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPW13248.1"
                     /translation="MAGVMALAYDAGMDSIAGTTLDTDRDSVLETVLGAYRMRVEITA
                     DVRYCGTWYDQEPATRFGQFHLLTEGECWVSGAALEQPLQLRRGDLIVFPAGVRHVLS
                     SGADPQLSAADDPDDTAMLCGELEFITGTSNPIFSALPAYFVVRGGDSSEGFRQLASM
                     LTETSRDRRWGRQLVQNKLADSLFTMAVCEYVRRADRPRGLLAALTDVRLSRALAAVH
                     ERPGEDWTIQSMAQCAGMSRTAFAELFTATVGLPPIQYLAQWRATEARRLLKNRRFSV
                     AAVAEMLGYRSEAAFRRFFKRIEGIGPGKARSSGSDDDAG"
     gene            2331..2627
                     /locus_tag="PK399_08765"
     CDS             2331..2627
                     /locus_tag="PK399_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13249.1"
                     /translation="MTTKTTTLSGLSLAVAAAALFLGAPIANAGETGTAKTGHCMGAN
                     ACKGHGACKSAANACKGQNACKGQGFTATTAKECQVAGGKFEMPKPAKAEAKKA"
     gene            2688..3533
                     /locus_tag="PK399_08770"
     CDS             2688..3533
                     /locus_tag="PK399_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HPW13250.1"
                     /translation="MTRTLHGFGLGLRPQHYDALLSADTNAVDWLEIVSENYLVDGGR
                     PLSMLDRIGERWPLAMHGVSLDIGGSDPLDRDYLRALGKLAKRVRPALVSDHLCWTRH
                     AGVQLHDLLPLPCNEDTLLHVATRVRQVQDALGRQLVLENVSSYLRFTSNTLEEAEFL
                     SALVADTGCGLLLDINNVYVNAHNHGIDAIRYLDVLPRHAVRQIHLAGHSEDALGSGL
                     LIDTHDAPVCDAVWALYAHAMQRFGGVPTMIERDDHIPPLAELLGELEIARGIAANTR
                     LERAA"
     gene            3530..4303
                     /locus_tag="PK399_08775"
     CDS             3530..4303
                     /locus_tag="PK399_08775"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HPW13251.1"
                     /translation="MSALATTQAAMHDWLRHGDPAIAARVEGPLVAHRLRIHADAYRL
                     RLIEVLANDYPVTAALLDEDRFDDAALGYLQAHPSTHPSLRHFGRGFAGWLQQAPAMP
                     RHLHELARFEWLQGEVFDAADAAPLTLDAIASLPAGAWPSLRLHLQPAVRFITLASNA
                     PALVEAHAQGRRLPALRRVPGAHWLLWRVDGDAHWRRLDADEAALLQAVFAGETFGDL
                     CERATDFHAHDGPLRAASVLKRWLADGLLAIDPPAPTHD"
     gene            4374..4826
                     /locus_tag="PK399_08780"
     CDS             4374..4826
                     /locus_tag="PK399_08780"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPW13252.1"
                     /translation="MPADTRPLAGIEATLRNYAWLGPLLLRLVFGWFWLETGWAKLHN
                     LEFFVGRFVEWGIPFPTLSATVSGATDLVGGALLIVGLGTRLAAIPMIINMLVALAVV
                     VLPTITTFNEFVELDEVLYVCVLFWLLMAGPGKASLDHLLARRFTKPA"
     gene            complement(4845..5870)
                     /locus_tag="PK399_08785"
     CDS             complement(4845..5870)
                     /locus_tag="PK399_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPW13253.1"
                     /translation="MRQMQMQRGGAIGLAALLLAACGAAPAPAVDASVSAPRTAAIEG
                     RPAAADVDALRERARQAMQASRLFAPAGDNALEHYLALRAQAPGDASVDAALVDLQPQ
                     LVIAIEQAIANGADPESRRLLALLARMDAQAPALPRLRATVANAETVARQRELAVDAE
                     ARDKAQRAEADLRRLAQERAARLAAERTTAAANNRPATSTPATVQPAPAPQAPPPTRM
                     ASEAPVRNAVAASAPPEAPVRQAAPPPVQAVNVATKPQDAPKLLRDVAPRYPDGGGRT
                     RQSGQVQVAFTINPQGGVESPRVVSSDLPTAFDRAALAAAARWKFEATGNSQPGTRTV
                     RFDPPGG"
     gene            complement(5992..6822)
                     /locus_tag="PK399_08790"
     CDS             complement(5992..6822)
                     /locus_tag="PK399_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13254.1"
                     /translation="MLKPFVALALLAPTASALAGECEANFRKSGNPLVMTTYSSAVTV
                     PNLATRDALAQMRGILVAEKMDVMAEDAEGGTLLVEQRQSGSARPIPTTINVSEVASG
                     TRIEMAVKMERGVFSKVEQIQPYMCGLFARVKGGKEGVAAARKGAGATNTEATGNQDV
                     FLFSRMIAREADKNAIAVNARHKGRKYTLTGRIDTLMEDGEDYNLVFDIPEISEMAIK
                     PLPFDAQFRVGVSCLFKPNQLATVLAMREGQKVTLTGTVYKYDSFRKVIWLENCTKAK
                     "
     gene            complement(6965..8938)
                     /locus_tag="PK399_08795"
     CDS             complement(6965..8938)
                     /locus_tag="PK399_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPW13255.1"
                     /translation="MPRFDAPLLARFRPLAWLGGCFLGISMLTRLVLLVATGNGVPAS
                     ASNWLYVFGVGLGYDLLAFAYFSVPLVLLLWLLPRRWLARRWGSTLVATLCLVLLSIL
                     LFVAVSEWTFWEEFQARFNFIAVDYLVYTTEVIGNIRESYPIGLILSSLALVTGGVFI
                     ATRRWRLVRNDSAGFGARSLVVAGWLAAGVLGTWLVSGDMKDRTANTYVNELAGNGIY
                     QFFAAYRSASLDYARYYRTVPADEARAIVRAQLATPDATFLATDGIARHIHNAAPERR
                     LNVVLISVESLSAEYSGVYGRKDSLTPNLDALAKDSLLFTDLWATGTRTVRGLEALAL
                     SVPPTPGESIVKRAHNEGLFSLGQVFRDRGYDAQFLYGGYGAFDDMNRFFAGNGYDVH
                     DRTEIADKDIHHANIWGVADEDLYTMAMRRFDADAAAGKPFFAHLMTTSNHRPYTFPA
                     GRGPWPQGKRESAVSYTDWAIGDFLRRARSKPWFANTLFVVTADHCASSAGIAALPAF
                     RYRIPMWVYSPGNVAPGRHERMTSQVDIAPTVLGLLGMDYDSRFYGVDVLQQPPGHER
                     AFIGTYQLLGYLRGGALVQLSPHRKVDTLRPSLERDQPQPALPEDPSLTLQAIAYYQD
                     AADAFASGAMRMPEPRRAGALARLQEPGEPPRA"
     gene            9074..9766
                     /locus_tag="PK399_08800"
     CDS             9074..9766
                     /locus_tag="PK399_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW13256.1"
                     /translation="MTQAAGNRLRVLVIEDQHDIAANIWDFLERRGHEVDHCADGVSG
                     LARAMRGDVDVVVLDLGLPRMDGLDLCRRLREAGNGVPILMLTARDTLEDKLRGFAEG
                     ADDYLVKPFAMRELEARIHAVRPHRAPSGGVPAFAGLTYDPRTMTAERDARRIPLTRL
                     QGSLLAVLLQEAPRIVPHARLIDTAWGAEGGDIAALQTQVYELRALVDKPFAQPLIHT
                     VRGIGYRLASPA"
     gene            9763..>10076
                     /locus_tag="PK399_08805"
     CDS             9763..>10076
                     /locus_tag="PK399_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13257.1"
                     /translation="MKPSMPLRRRFTLLATVMGLLLSTLSAATIAFVAEDYEYVIATE
                     IMHGQAEDYGLRLANGLPAELPRTQRLSGYRIDAQELPRAYTTYAPGVREDPAHEDIH
                     VGV"
BASE COUNT         1552 a         3492 c         3442 g         1590 t
ORIGIN      
        1 gccggcggcg ggctggtgcc gtgggcggtg ctgtccggct acggcaccga cacccaggac
       61 ggcggcaccg cgttcgcgac ccgggtcgac accggcgact attcgctgga tgcggcgggc
      121 gtcgcctaca cgttccgcaa ccgcatcgaa ctctcctacg cgcggcagcg cttcgggctg
      181 ggcgaactgc aacgccggct ggccttgccg gtgggccggt tcgacgccga ggtgatcggc
      241 gcgaaggtgc gcctgggcgg cgacctggtg tacaccgcga tgccgcaact ctcgttcggc
      301 gtgcagcaca agcgccagct ggatttcgcg atcccgtccg cggtcggcgc gcgcagcgac
      361 agcggcaccg acgtgtacct cgcggccagc aaactgttcc tgggcgcggc cttcgatcgc
      421 aatctgctgt tcaacgcgac gctgcgcagc accagcgcga accaggccgg cttgctcggc
      481 ttcggcggcg accgcaaggc cggtcgttcg ctggtgctgg aatcgtcggt ggcgttgctg
      541 ctggacccac gctgggcggt cggcgtggag tatcggcaga agccggacaa cctcggcttc
      601 gcccgcgagg acgactggaa ggacgccttc gtggcctggt tcccgaacaa gcgcgtcgcc
      661 gtggtcgccg cctacgcgga cctcggctcg atcgccacgc tggacaggca gcgcgggccc
      721 tatctctccc tgcaggcgtc gttctgatgg caacgcatcc attccgcctc gcatgcctgt
      781 cggtgctgtt gccggcgttc ctgggcgcct gtgcgagcca gcccaggacc acgacctacg
      841 accagctggg cggtgccgcc ggcatcgaag gcatcgtcga tgcgctgctg gagaagatca
      901 tcgaggacga acggatcaat ttccagttcg ccgacaccga catcgtgcgc ctgcgcagca
      961 tgctgatcga acagttctgc gcggaatccg gtggaccgtg catgtacagc ggcctgtcga
     1021 tgcaggagtc gcacgcgggt cgcaacatcg acactgcgca gttcaacgca ctggtcgagg
     1081 acctcatcga cgtgatgacc gcgcgcagga tcccggtggg tgcccagaac cgcctgctca
     1141 ggcggctggc gccgatgcac ggtgagatcg tcacgccctg atcgcggcgg cgctcaaccc
     1201 gcgtcgtcgt cgctgccgct gctgcgcgcc ttgccggggc cgatgccctc gatgcgcttg
     1261 aagaagcggc ggaacgcggc ctcgctgcga tagcccagca tctccgcgac cgcagccacc
     1321 gagaagcgtc ggttcttcag caatcggcgc gcttcggtcg cgcgccactg cgccaggtac
     1381 tggatcggcg gcaggccgac ggtggcggtg aacagttcgg cgaaggccgt gcgcgacatg
     1441 ccggcgcact gtgccatcga ctggatcgtc cagtcctcgc ccgggcgttc gtgcactgcc
     1501 gccagcgcac gcgacaggcg cacgtcggtc agcgccgcca gcaagccacg cggccggtcg
     1561 gcacggcgca cgtactcgca caccgccatc gtgaacagcg aatcggccag cttgttctgc
     1621 accagttgcc ggccccagcg acggtcgcgg ctggtttcgg tgagcatgct cgcgagctgg
     1681 cggaagcctt cgctgctgtc gcctccacgc acgacgaaat acgccggcag cgcgctgaag
     1741 atcggattgc tggtgccggt gatgaattcc agctcgccgc acagcatcgc agtgtcgtcg
     1801 ggatcatcgg ccgccgagag ctgcgggtcc gcacccgacg acagcacatg gcggacaccc
     1861 gccgggaaca cgatgaggtc gccccggcgc agttgcagcg gctgttccag cgccgcgccg
     1921 ctgacccagc attcgccttc agtgagcagg tggaactggc cgaagcgggt cgccggctcc
     1981 tggtcgtacc aggtgccgca atagcgcaca tcggcggtga tctccacgcg catgcggtac
     2041 gcgcccagga cggtctccag cacgctgtcg cgatcggtgt cgagggtcgt gccggcgatg
     2101 ctgtccatgc cggcatcgta ggccagcgcc atcaccccgg ccatgtccgt gccgccggcg
     2161 tttcatgcgc cagcgtccgc cggcgctgaa ccggggcccg ctggcggacg cacgggcata
     2221 tccacgggac tggcggggat atcggcagga gcggcatcgg aatggaatac acaggcgccg
     2281 gaaccacccc gcccggcgtc cccaaaccca cccgaaccga ggattcccgc atgactacca
     2341 agaccaccac cctgtccggc ctgtcgctcg ccgttgccgc cgccgcgctg ttcctgggcg
     2401 cccccatcgc caatgccggc gagaccggca ccgccaagac cggccattgc atgggcgcga
     2461 atgcctgcaa gggccacggc gcctgcaaga gcgcggccaa tgcctgcaag gggcagaacg
     2521 cgtgcaaggg ccagggcttc accgcgacca ccgccaagga atgccaggtg gccggcggca
     2581 agttcgagat gcccaagccg gccaaggccg aagcgaagaa ggcctgatcc cgacgcccac
     2641 gcgcgcgtcc gttccccgac ggacgcgcgc accgagggat ggatgacatg acccgcaccc
     2701 tgcacggctt cggcctgggc ttgcgcccgc agcactacga cgccttgctg tcggccgaca
     2761 ccaacgccgt cgactggctg gaaatcgtca gcgagaacta cctggtcgat ggcggccgcc
     2821 cgctgtccat gctcgaccgg atcggcgagc gctggccgct ggcgatgcat ggcgtctcgc
     2881 tggacatcgg cggcagcgat ccgctcgacc gcgactacct gcgcgcgctt ggcaagcttg
     2941 cgaaacgcgt gcgccctgca ctggtctccg accacctgtg ctggacccgc catgccggcg
     3001 tgcagctgca cgacctgttg ccgctgccct gcaacgagga caccctcctc cacgtcgcga
     3061 cgcgcgtccg ccaggtgcag gacgcactgg gcaggcaact ggtgctcgaa aacgtgtcca
     3121 gctacctgcg cttcaccagc aacacactgg aggaggccga gttcctgtcc gcgctggtcg
     3181 cggacaccgg ctgcggcctg ctgctcgaca tcaacaacgt ctacgtcaac gcgcataacc
     3241 atggcatcga cgccatccgg tatctcgatg tgctgccgcg acacgcggtg cgacaaatcc
     3301 acctggccgg acacagcgaa gacgcgctcg gctccggcct gctgatcgat acccatgacg
     3361 caccggtctg cgatgccgtg tgggcgttgt acgcgcacgc gatgcagcgc ttcggcggcg
     3421 tgccgacgat gatcgagcgc gacgaccaca tcccgccgct ggcggagttg ctgggcgaac
     3481 tcgagatcgc cagagggatc gccgccaaca cgcggctgga gcgcgccgca tgagcgcgtt
     3541 ggccaccacc caggcggcga tgcacgactg gctgcggcat ggcgatcccg ccatcgccgc
     3601 gcgggtcgaa ggcccgctgg tcgcgcatcg cctgcgcatc cacgcggacg cctaccgcct
     3661 gcgcctgatc gaggtgttgg ccaacgacta cccggtgacc gccgcgctgc tggacgagga
     3721 ccgcttcgac gacgccgcac tcggctacct gcaggcgcat ccgtcgacgc atccgtcgct
     3781 gcgccacttc gggcgcggct tcgctggttg gctgcagcag gcgcccgcga tgccgcgcca
     3841 cctgcatgaa ctcgcacgct tcgaatggtt gcaaggcgaa gtgttcgatg ccgcggatgc
     3901 cgcgccactc acgctcgacg ccatcgcgtc attgcctgcc ggcgcgtggc cgagcttgcg
     3961 cctgcacctg cagccggcgg tgcgcttcat cacgctggcc tccaacgcac cggcactggt
     4021 cgaggcgcat gcgcaaggac gccgtctgcc cgcgttgcgc cgcgtaccag gcgcgcactg
     4081 gctgctgtgg cgcgtcgatg gcgacgcgca ctggcgcagg ctggatgccg acgaggccgc
     4141 cctgctccag gccgtgttcg ccggcgaaac cttcggtgac ctgtgcgaac gggcgaccga
     4201 cttccacgca cacgatggtc cgctgcgcgc agccagcgtg ctcaagcgct ggctggccga
     4261 cggcctgctg gccatcgatc cacctgcccc cacccacgat tgaggacacg cccatgcaag
     4321 gttgcgcatt cccttccaat attcgccgcc gtttgcgcga acgcgcgaac gcgatgccgg
     4381 cggacacgcg cccgcttgcc ggcatcgagg ccacgctacg caactacgcg tggctgggcc
     4441 cattgctgct gcggctggtg ttcggctggt tctggttgga aaccggctgg gccaagctgc
     4501 acaacctgga gttcttcgtt ggtcgcttcg tcgaatgggg gattccgttt ccgaccctga
     4561 gcgccaccgt atccggtgcc accgacctcg ttggcggtgc gttgctgatc gtcggcctgg
     4621 gcacccggct ggccgcgatc ccgatgatca tcaacatgct ggtggcattg gcggtggtcg
     4681 tgttgccgac catcaccacc ttcaacgaat tcgtcgaact ggatgaagtg ctgtacgtct
     4741 gcgtgctgtt ctggctgctg atggccgggc ccggcaaggc cagcctggat catctgctgg
     4801 cgcggcgctt cacgaagccc gcatgacgcc tgcgccggcg cggctcagcc gcccggcgga
     4861 tcgaagcgca ccgtgcgcgt gcccggctgg ctgttgccgg tggcctcgaa tttccagcgc
     4921 gcggcggcgg ccaacgcggc acggtcgaac gcggtcggca aatcggacga caccacccgc
     4981 ggcgactcca cgccaccctg cggattgatg gtgaaggcga cctgcacctg gccggactgg
     5041 cgggtgcgcc cgccgccatc gggatagcgc ggggccacgt cgcgcaacag cttcggtgcg
     5101 tcttgcggct tcgttgcgac gttaacggct tggacgggcg gtggcgctgc ctgtcggaca
     5161 ggcgcttctg gtggggccga cgccgcgaca gcgttgcgga cgggtgcctc gctggccatg
     5221 cgagtcggtg gcggagcttg cggcgcgggc gcgggctgca cggtggcggg cgtggatgtc
     5281 gccggtcgat tgttggcggc ggcggtcgtg cgttccgcgg ccagtcgcgc cgcgcgttcc
     5341 tgggccagtc ggcgcaggtc ggcctcggcg cgctgcgcct tgtcgcgcgc ctcggcatcc
     5401 accgcgagtt cgcgctggcg ggcgacggtt tcggcattcg ccacggtggc gcgcaggcgc
     5461 ggcagcgccg gtgcctgcgc atccatccgc gcaagcagcg caagcaagcg gcggctttcc
     5521 ggatcggcgc cgttggcgat ggcctgctcg atcgcgatca ccaactgtgg ttgcaggtcg
     5581 accagcgcgg catcgacgct cgcatcgccc ggtgcctgcg cgcgcagggc caggtaatgc
     5641 tccagcgcgt tgtcgcccgc cggtgcgaac agcctggacg cctgcatcgc ctggcgcgcg
     5701 cgttcgcgca aggcatcgac atcagccgcc gcaggcctgc cttcgatcgc cgccgtgcgc
     5761 ggtgcggaga cggaagcatc gacggcaggc gcaggcgcgg ccccgcatgc ggcaagcagg
     5821 agcgcagcca gcccgatcgc accgccccgt tgcatctgca tctggcgcat gacccatccc
     5881 cgtggaaatt cgggggagcc tggcgcgcga tcatgacagc gcgatgttgg ccggatgtcg
     5941 caacacggaa acgcgaagcg cgtcacgtcg agtggcggcc ccgccgttgc atcactttgc
     6001 cttggtgcag ttctccagcc agatcacctt gcggaagctg tcgtacttgt agaccgtacc
     6061 ggtgagggtg actttctggc cttcgcgcat cgccagcacg gtggccagct ggttcggctt
     6121 gaacaggcag gacacgccga cccggaattg cgcatcgaac ggcagcggct tgatcgccat
     6181 ctcgctgatc tcggggatgt cgaagaccag gttgtagtcc tcgccgtcct ccatcagcgt
     6241 gtcgatgcgc ccggtcaggg tgtacttgcg gcccttgtgg cgtgcgttga ccgcgatggc
     6301 gttcttgtcg gcttcgcgcg cgatcatccg cgagaacagg aagacgtcct ggttgccggt
     6361 ggcctcggtg ttggtcgcac ccgcgccctt cctggccgcg gccacgcctt ccttgccacc
     6421 cttgaccctg gcgaacaacc cgcacatgta gggctggatc tgctcgacct tggagaacac
     6481 cccgcgctcc atcttcaccg ccatctcgat gcgggtgccc gacgccactt cgctcacgtt
     6541 gatcgtggtc gggatcggac gcgcgctgcc ggattggcgt tgctcgacca gcagggtgcc
     6601 gccctcggcg tcttcggcca tcacgtccat cttctcggcg accaggatcc cgcgcatctg
     6661 cgccagcgca tcgcgcgtgg ccaggttcgg cacggtgact gcactgctgt aggtggtcat
     6721 caccagcgga ttgccggact tcctgaagtt ggcctcgcat tcgccggcga gggcggatgc
     6781 ggtcggcgcc aacagcgcga gggcaacgaa tggcttgagc atgtggcgtc ctggtcgtgg
     6841 cgtcgggaac ggggcaccga tgctaaggac tgcgcgcatc gccagcttgt atcgcgcagt
     6901 gatcgatgcc gttcgccccc tcaaccgatg cgttggtcac cgcaggctgg gtggcccgat
     6961 gccgtcatgc gcggggcggt tcaccgggct cctgcagcct cgccaatgcg cccgcacgac
     7021 gcggctcggg catgcgcatc gccccgctgg cgaacgcgtc cgcggcatcc tggtagtagg
     7081 cgatcgcctg cagggtcagc gacggatcct cgggcagggc cggttgcggc tggtcgcgtt
     7141 ccagcgacgg ccgcagggtg tcgaccttgc ggtgcggcga cagctgcacc agggcgccgc
     7201 cgcgcaggta gcccagcaac tggtaggtgc cgatgaaggc gcgctcatgg ccgggcggct
     7261 gctgcagcac atccacgccg tagaagcggc tgtcgtaatc catgcccagc aggccaagca
     7321 cggtcggcgc gatgtcgacc tggctggtca tccgttcgtg gcgaccgggc gcgacgttgc
     7381 ccggcgaata cacccacatg ggaatccggt accggaacgc aggcaatgcc gcgatgccgg
     7441 cgctcgaggc gcagtggtcg gcggtgacca cgaacagggt gttggcgaac cacggcttgc
     7501 ttcgcgcgcg gcgcaggaaa tcgccgatcg cccaatccgt gtaactcacc gcgctctcgc
     7561 gcttgccctg cggccacggt ccacgcccgg ccgggaaggt gtagggccgg tggttggagg
     7621 tggtcatcag gtgcgcgaag aacggcttgc ccgcggcggc atcggcatcg aaacgacgca
     7681 tcgccatggt gtacaggtct tcgtcggcga cgccccagat gttggcgtgg tggatgtcct
     7741 tgtccgcgat ctcggtgcgg tcgtgcacgt cgtagccgtt gccggcgaag aaccggttca
     7801 tgtcgtcgaa cgcgccgtag ccgccgtaca ggaattgcgc gtcgtagccc ctgtcgcgga
     7861 acacctggcc cagcgagaac aggccttcgt tgtgcgcgcg cttgacgatg gactcgcccg
     7921 gcgtgggcgg cacggacaac gccagtgctt ccagtccgcg cacggtgcgc gtgccggtcg
     7981 cccacaggtc ggtgaacagc aggctgtctt tcgccagcgc gtccaggttc ggggtcaggc
     8041 tgtccttgcg gccgtacacg ccggagtatt cggcggacag gctttccacg ctgatcagga
     8101 ccacgttgag gcgccgctcc ggggcagcgt tgtggatgtg gcgggcgatg ccgtcggtgg
     8161 cgaggaaggt ggcgtccggc gtggccagct gggcgcgcac gatcgcccgc gcctcgtccg
     8221 ccgggacggt ccggtagtag cgcgcgtagt ccagcgaggc gctgcgatac gcggcgaaga
     8281 actggtagat gccgttgccg gcgagctcgt tgacgtaggt attggcggtg cggtccttca
     8341 tgtcgccgct gaccagccag gtgcccagca cgcctgccgc cagccacccc gcgaccacca
     8401 ggctgcgcgc gccgaagccg gcgctgtcgt tgcgcaccag ccgccagcgg cgggtggcga
     8461 tgaacacgcc gccggtcacg agcgccagcg acgacaggat caggccaatc gggtaggact
     8521 cgcggatgtt gccgatgacc tcggtggtgt agacgaggta gtcgaccgcg atgaaattga
     8581 agcgcgcctg gaattcctcc cagaacgtcc attccgagac cgccacgaac aacaggatcg
     8641 acaacagcac caggcacagg gtggcgacca gcgtgctgcc ccagcgccgc gccagccagc
     8701 gccgcggcaa cagccacagc agcaggacca gcggcacgct gaaataggcg aacgccagca
     8761 ggtcgtagcc caggcccacg ccgaacacgt acagccagtt cgatgcggag gcgggcacgc
     8821 cgttgccggt ggccaccagc aggaccaggc gggtcagcat cgaaatgccc aggaagcacc
     8881 cgcccagcca cgcaagcgga cggaagcggg caagcagcgg agcgtcgaag cgtggcatcg
     8941 ggcgggtgcg cgggcggtgg tgcggcgcat gctgcgggcc accgcgtaat gccggctcag
     9001 gcgaatctca gcgaatcgtt aacccgtcct cgcgccgtcg gtggcaggct atgcaggcat
     9061 acggagccgc accatgaccc aagccgccgg caaccgcctg cgcgtgctgg tgatcgaaga
     9121 ccagcacgac atcgccgcca acatctggga cttcctcgag cgccgtggcc acgaggtgga
     9181 tcactgcgcc gatggcgtat ccgggttggc gcgggcgatg cgcggcgatg tcgacgtcgt
     9241 cgtactcgac ctcggcctgc cgcggatgga cggactggac ctgtgccgcc gcctgcgcga
     9301 ggccggcaac ggcgtaccta tcctgatgct gaccgcacgc gacaccctcg aagacaagtt
     9361 gcgcggcttc gccgaaggcg ccgacgacta cctggtcaag ccgttcgcga tgcgcgaact
     9421 ggaggcgcgg atccatgcgg tgcgcccgca tcgcgcgccg tccggcggcg tccctgcctt
     9481 cgccgggctg acgtacgatc cgcgcacgat gaccgccgaa cgcgacgccc gccggatccc
     9541 gctgacccgc ctgcaggggt ccctgctggc cgtcctcttg caggaggcac cgcggatcgt
     9601 gccgcatgcg cgcctgatcg acaccgcctg gggtgcggaa ggcggcgata tcgccgccct
     9661 gcagacccag gtctatgaat tgcgcgcgct ggtcgacaag ccgttcgcgc agccgttgat
     9721 ccacaccgtg cgcggcatcg gttaccggct ggcatcgccc gcatgaagcc gtcgatgccg
     9781 ctgcgccgca ggttcaccct gctcgccacc gtgatgggct tgctgttgag cacgctgtcc
     9841 gccgcgacca tcgccttcgt cgccgaggac tacgaatacg tgatcgccac cgaaatcatg
     9901 catggccagg cggaggacta cggcttgcgc ctggccaacg ggttgcccgc ggaattgccg
     9961 cgcacccaac gcctgagcgg ctaccgcatc gatgcccagg aactgcccag ggcttacacc
    10021 acctatgcgc ccggcgtgcg cgaggatccc gcccacgagg acatccacgt cggcgt
//
LOCUS       DAOOCX010000088         9897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_6467_length_9897_cov_9.035867, whole genome shotgun sequence.
ACCESSION   DAOOCX010000088 DAOOCX010000000
VERSION     DAOOCX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6467_length_9897_cov_9.035867"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /gene="asnB"
                     /locus_tag="PK399_08810"
     CDS             complement(<1..1635)
                     /gene="asnB"
                     /locus_tag="PK399_08810"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HPW13258.1"
                     /translation="MCSIFGIFGLQPGDDIQALRRQSLDCSQRQRHRGPDWSGVYIDE
                     GGASEGAILVHERLAIVDPAGGSQPLLSEDRGLALAVNGEIYNHKQLKAELKQAYAFQ
                     TESDCEVINALYLEDAPASFLNRLNGIFAFALWDAGKGRAIIARDPLGVCPLYWGHDK
                     EGRLRVASEMKSLVDECADVAQFPPGHWYDTATGELTKYYQRPWRDYDVVEGVEVSLQ
                     ELREAFEAAVHRQLMTDVPYGVLLSGGLDSSLVAAVAARYARHRIEDGDRTEAWWPRL
                     HSFAIGLKGSPDLAAAKIAAEALGTVHHGFEYTFEEGLDALPEVIRHIETYDVTTIRA
                     STPMFLLARRIKAMGVKMVLSGEGSDEIFGGYLYFHKAPNAREFHEELIRKLDALYNY
                     DCLRANKSMMAWGVEPRVPFLDREFLDVAMRIDAKYKMVDKSRSDAQRMEKGILRQAF
                     DGHLPESILWRQKEQFSDGVGYGWIDGLKAHAEAQVSDRELAAAGKRFPVNPPQTKEA
                     YFYRSVFERFYPTPAAADTVPGGKSIACSSPAAIAWD"
     gene            complement(1941..3077)
                     /gene="dapE"
                     /locus_tag="PK399_08815"
     CDS             complement(1941..3077)
                     /gene="dapE"
                     /locus_tag="PK399_08815"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HPW13259.1"
                     /translation="MSDVLQLTIDLIARTSVTPDDAGCQRLIGERLQRAGFSIESLRF
                     GEVDNLWATHGDGDGPVLVLLGHTDVVPTGPREAWASDPFAPEIRDGVLYGRGAADMK
                     GSVAAFVVALEAFVAAHPRHAGKVALLLTSDEEGDAIDGVRKVADVFRERGERIDWCI
                     TGEPSSKHALGDLLRVGRRGTLSATLTVRGIQGHVAYPDKARNPIHQAMPALAALAAK
                     RWDEGYESFPPTSLQVSNINAGTGANNVIPGELQALFNLRYNPHWNAAQLESECEAVL
                     RAYGLEYSVHWHRGGEPFHTPEGALRQAAREVLATFAGGALPEESTGGGTSDARFIAP
                     LGAQCIEVGPVNASIHKVDEHVRVADLERLPGLYLRLMERLLVA"
     gene            complement(3121..3480)
                     /locus_tag="PK399_08820"
     CDS             complement(3121..3480)
                     /locus_tag="PK399_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HPW13260.1"
                     /translation="MTTLYGLKNCDTCKKALNWLKRFGIEHRFVDYRDERQSPDTLVA
                     WKDAAGGWDTLINKSSTTWRGLSPNRKTPGSDAEWKLLLKEYPQLMRRPVVVLDDGTV
                     AQGFSDNGFKKLFDIRN"
     gene            complement(3483..4310)
                     /gene="dapD"
                     /locus_tag="PK399_08825"
                     /pseudo
     CDS             complement(3483..4310)
                     /gene="dapD"
                     /locus_tag="PK399_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017115329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
     gene            complement(4352..6934)
                     /gene="glnD"
                     /locus_tag="PK399_08830"
     CDS             complement(4352..6934)
                     /gene="glnD"
                     /locus_tag="PK399_08830"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HPW13261.1"
                     /translation="MPPAVDADPAWAAAARAWLVQSDARFARRFDAGEPADKLVALRA
                     RAVDTLVRDAWQRCIPEGDALALFAVGGYGRGELFPQSDIDLLVLAEPDVQRAAQPAL
                     ARFVALLWDAGLQASPAVRSAAECTEAAADQTVLTALIEARPLVAWEEETAALRATIA
                     PDRAWAPRAFLEAKVAEQRQRHARFGDTSDNLEPNIKDGPGGLRDLHMLGWLALRSFG
                     VRDLDALVGLGHLGPDEAIALERERRVLCRLRYGLHLVAGRREERLRFDYQKLLAARL
                     GYEDDGDSLAVEKMMQGFYRSAAIVRRINDRLLQRFEEHFDGSALAEPIDGDFERRRG
                     YLATRVPGWLLRDPANVFALFATWAADTGLRGLHSQTARELAQALPGIPAYTDVEPTV
                     RARFMALLRGPQPVKVLERMARLGVLGRWIPAFAEVSGRMQFDLFHVYTVDQHTLAVL
                     KNLAAFASGQGDPRFSIAHRVWPSLRKPELLLLAGLFHDIGKGRGGDHSELGAVDARA
                     FCEAHDLSEADTAQVEWLVRRHLLMSVTAQKQDITDPEVIHRFAGEVADRQRLDYLYL
                     LTCADIAGTSPKLWNDWKDRLLADLHTATRLALRRGLEHPVAAEQRIEENRAAARALL
                     SASGDDDASMEQVFARMPRDNFLRSRPDQIAWQVRVMHAAPGDRPVVGVRYPTGREGL
                     EVFVHMRDRDGLFAALVIALDRMGLGVLQARVLDGPDGTIFDTFHVQPMDTRIAPVST
                     QVEAQLAKALSGDLACLQPARRSQPRHLRHFRIAPQLDFTEIAGGTRIGLVCNDRPGL
                     LADIARVLREQRLRVHDARIATFGERAEDLFLVSDEHDHSLQPAQRDALRDALLAALD
                     GEPR"
     gene            complement(6985..7761)
                     /gene="map"
                     /locus_tag="PK399_08835"
     CDS             complement(6985..7761)
                     /gene="map"
                     /locus_tag="PK399_08835"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPW13262.1"
                     /translation="MSIQLKSAADLDKMREAGRLAAEVLQIVAPFVKPGVTTEELDRI
                     CHDHIVQVQKAIPANVGYRGFPKTVCTSVNNVICHGIPSEAKVLKDGDIVNIDVTVIK
                     DGWHGDTSRMYIVGTPSVMAQRLVDVTREAMFRGIRAVKPGATLGDVGHAIQQYAESQ
                     RFSVVREYCGHGIGKIYHDDPQVLHYGQPGQGVVLKEGMTFTIEPMINEGSRHTRVLP
                     DGWTVVTKDRKLSAQWEHTVAVTADGVEILTRLPGDDNDL"
     gene            8058..8840
                     /gene="rpsB"
                     /locus_tag="PK399_08840"
     CDS             8058..8840
                     /gene="rpsB"
                     /locus_tag="PK399_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPW13263.1"
                     /translation="MPQITMRQMLEAGVHFGHQTRYWNPKMAPYIFGARGKIHIINLE
                     KTVPLFNDAMNFISGVAQKRGTVLFIGTKRSARDPIKEEAERCGMPYMTQRWLGGTLT
                     NFQTVKKSVSRLKELEAAETDGTFQKLVKHEVMGLRREREKLQASLGGIKDMNRLPDA
                     LFVIDIGHEDIAIKEAKKLGIPVIAVVDSNYDPALVDYAIPGNDDAIRAVQLYARAAA
                     DSVLEGKAATPSAATIREEDFAAAPEGEDKKPARRAPKPKAE"
     gene            9013..9891
                     /gene="tsf"
                     /locus_tag="PK399_08845"
     CDS             9013..9891
                     /gene="tsf"
                     /locus_tag="PK399_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPW13264.1"
                     /translation="MADISASLVKELRERTGAGMMECKKALTENNGDIDASAEWLRKS
                     GLAKADKKASRVAAEGRIAVAQSAGKAVLVEINSETDFVAKDDNFVAFTDAVAQAALD
                     SGATDVEALKSAKLASGETVEEARAAAIAKLGENILVRRIASITTGNNVAAYSHGGKI
                     GVLVEVAGGDVDFARGIAMHVAAMNPPYNKAADVPADFVAKEKEIELAKMSDKDKQKP
                     AEILEKIIGGKIAKIVNEVSLYGQPYVLNTDHSVEQAAKAAGADVVSFTRLAVGEGIE
                     KVVEDYAAEVAKAMQV"
BASE COUNT         1623 a         3463 c         3284 g         1527 t
ORIGIN      
        1 gtcccaggcg atcgccgccg gcgacgaaca ggcgatcgac ttgccgcccg gcacggtgtc
       61 cgccgccgcg ggcgtgggat agaagcgctc gaacacgctg cggtagaaat acgcttcctt
      121 ggtctgcggc gggttgaccg ggaagcgctt gccggcggcg gccagttcgc gatcgctgac
      181 ctgcgcctcg gcatgcgcct tcagcccgtc gatccagcca tagccgacgc cgtcgctgaa
      241 ctgttccttc tgccgccaca ggatcgactc gggcaggtgg ccgtcgaagg cctgtcgcag
      301 gatgcccttc tccatccgct gggcgtcgct tcggctcttg tcgaccatct tgtatttcgc
      361 gtcgatgcgc atcgccacgt cgaggaactc gcggtccagg aacggcaccc gcggctccac
      421 gccccaggcc atcatcgact tgttggcgcg caggcagtcg tagttgtaga gcgcatccag
      481 cttgcggatc agttcctcgt ggaactcgcg cgcattcggc gccttgtgga agtacaggta
      541 gccgccgaag atctcgtcgc tgccctcgcc gctcagcacc atcttcaccc ccatcgcctt
      601 gatccgccgt gccagcagga acatcggcgt ggacgcacgg atggtggtga cgtcgtaggt
      661 ctcgatgtgg cggatcacct cgggcaacgc atccaggcct tcctcgaagg tgtattcgaa
      721 gccatggtgc acggtgccca gcgcctcggc cgcgatcttc gccgccgcca ggtcgggcga
      781 gcccttcagg ccgatcgcga aactgtgcag gcgcggccac caggcctcgg tcctgtcgcc
      841 atcctcgatg cgatggcgcg catagcgcgc ggccacggcg gcgaccagcg acgaatccag
      901 cccgccggac agcagcacgc cgtacggcac gtcggtcatc agctggcggt gcaccgcggc
      961 ctcgaaggct tcgcgcaatt cctgcagcga cacctcaacg ccttccacca cgtcgtagtc
     1021 ccgccacggg cgctggtagt acttcgtcaa ttcgccggtc gcggtgtcgt accagtggcc
     1081 cggcgggaac tgcgccacgt cggcgcattc gtccaccagc gacttcattt ccgaggccac
     1141 ccgcaggcgg ccctccttgt cgtggcccca gtacagtggg cagaccccga gcgggtcgcg
     1201 ggcgatgatc gcgcgaccct tgccggcatc ccacaatgcg aaggcgaaga tcccgttgag
     1261 gcggttgagg aacgatgccg gcgcgtcctc gaggtacaac gcgttgatca cttcgcagtc
     1321 ggactcggtc tggaaggcat aggcctgctt cagttccgcc ttgagctgct tgtggttgta
     1381 gatctcgccg ttgaccgcca gcgcgagtcc gcgatcctcc gacagcagcg gctgcgagcc
     1441 gccggccgga tcgacgatgg ccaggcgctc atggaccagg atggcccctt cagacgcacc
     1501 accttcatcg atgtacaccc cgctccagtc cgggccgcga tgccgctggc gctgcgagca
     1561 atccagcgac tggcgtcgca gggcctggat gtcgtcgccg ggctgcagcc cgaagatgcc
     1621 gaagatggag cacatggggt ggttcctcag gattctcgaa acaaaaaaaa cccgcacttc
     1681 gcagtacggg ctcagggggt tgtgcgtgtg tcgcgcgcta accgccggcc cgtccgggtc
     1741 gattattcag gcgattggca ttagtgcgca gcgaggtcat gggccgatcc tgcaccaccg
     1801 cccggccgct gacaagcgac gttttcgcct gtaacgaatg cgaggctggc gacgcgattc
     1861 ggccgcacgt ccgcggcgat cgtcgaacca ccgccgccac ggcgccggcc tggtgcgcgg
     1921 cgcgctcgat cgacgaacgc tcacgcgacc agcaggcgct ccatcagccg aaggtagagc
     1981 ccgggcaagc gctcgaggtc ggccacgcgc acatgctcgt cgaccttgtg gatgctcgca
     2041 ttgaccgggc cgacttcgat gcactgcgca cccaatggcg cgatgaagcg cgcatcggag
     2101 gtgccgccgc cggtgctttc ctccggcaac gcaccgccgg cgaaggtcgc cagcacttcg
     2161 cgtgccgcct ggcgcagcgc gccttccggc gtgtggaagg gctcgccacc gcgatgccag
     2221 tgcaccgaat actccaggcc atacgcacgc agcaccgcct cgcattcgga ttccagctgc
     2281 gcggcgttcc agtgcgggtt gtagcgcagg ttgaacaagg cctgcagctc gcccgggatg
     2341 acgttgttgg cgccggtgcc ggcgttgatg ttgctgacct gcagcgaggt cggcgggaaa
     2401 ctttcgtacc cctcgtccca gcgcttggcc gccagcgcgg cgagcgcggg catcgcctgg
     2461 tggatggggt tgcgcgcctt gtccggatac gccacgtggc cctggatgcc gcgcaccgtg
     2521 agggtggccg acagcgtgcc gcgccggccg acccgcaaca ggtcgcccaa tgcatgcttc
     2581 gacgacggct cgccggtgat gcaccagtcg atgcgctcgc cgcgttcgcg gaacacatcg
     2641 gcgaccttgc gcacgccgtc gatcgcatcg ccttcctcgt ccgaggtcag cagcaaggcg
     2701 accttgcccg catgccgcgg atgcgcggcc acgaacgcct ccagcgcgac cacgaacgcc
     2761 gccacgctgc ccttcatgtc cgccgcgccg cgcccgtaca gcacgccgtc gcggatctcc
     2821 ggtgcgaacg gatcgctggc ccaggcctca cgcggaccgg tcgggaccac gtcggtatgg
     2881 ccgagcagca ccagcaccgg gccgtcgccg tcgccatgcg tcgcccacag attgtcgacc
     2941 tcgccgaaac gcagcgattc gatgctgaag ccggcgcgct gcaggcgttc gccgatcagg
     3001 cgctggcagc cggcatcgtc cggggtgacc gaagttcgcg cgatcaggtc gatcgtgagt
     3061 tgcaggacgt cgctcattgc caccctcacc ccagccctct cccgccagcg ggagagggag
     3121 tcaattgcgg atatcgaaca gcttcttgaa cccgttgtcg ctgaatccct gcgccaccgt
     3181 gccatcgtcc agcaccacca ccggcctgcg catcagctgc ggatattcct tcagcagcaa
     3241 tttccattcg gcatcgctgc ccggcgtctt gcggttcggc gacaacccgc gccatgtcgt
     3301 ggacgatttg ttgatcaacg tgtcccaacc accggccgcg tccttccacg ccaccagggt
     3361 gtccggcgat tgccgctcgt cgcggtaatc gacgaagcga tgctcgatgc cgaagcgctt
     3421 cagccagttc agcgccttct tgcaggtgtc gcagttcttc aggccgtaga gcgtggtcat
     3481 gctcaatccg ccaacccgcg cagcaactcg ttcaccgagg tcttgctgcg cgtgcgctcg
     3541 tcgacctgct tgacgatcac cgcgcaatac agcgaatgac tgccgtcctt cgccggcagc
     3601 tggccgctga cgaccacgct gtacggcggc accgagccgt agctgacctc gccggtggcg
     3661 cggttgtaga cgcgcgtgct ctggcccagg aacacgccca tgccgatcac gctgtggtgg
     3721 ccgaccacga agccttccac tacttccgag cgcgcgccga tgaaacagtg gtcttcgatg
     3781 atcgtcggcg acgcctgcag cggctccagc acgccaccga tgccggcgcc gccggacaaa
     3841 tggcaatgcg cgccgatctg cgcgcaggaa ccgaccgtgg cccaggtatc gaccatcgtg
     3901 ccttcgccga cataggcacc gatgttgacg aagctcggca tcagcaccac gtccttgccg
     3961 atgtggctgc ccgcacgtac gacagcgccc ggcaccacgc gggcgccgag cgcgcggaac
     4021 gcggcctcgt cgaacccggc gaagcgcgac ggcaccttgt cccagaacgg cgacggcgac
     4081 gcatccacca ggctcatgtc ctgggtgcgg aagtacagca gcaccgcctt cttcagccat
     4141 tcgttgaccc gccagccacc gctgccgtcg ggctcggcga cgcgcagcca gccctgctcc
     4201 agcccggcga tcgcgcggtc cacggcggga cgcgtggtcg cggcgatctc gtcggcggtc
     4261 agctccgcgc ggcgctccca ggcatcgtcc agcacggcac gcaatgcatc ggcatcgacc
     4321 acgccggtca gtggcgtggc atcaggttgg gtcatcgcgg ttctccgtcg agggcggcca
     4381 gcaaggcgtc gcgcagggcg tcgcgctggg cgggttgcag ggaatggtcg tgttcgtcgc
     4441 tgaccaggaa caggtcttcc gcgcgctcgc cgaaggtggc gatgcgcgcg tcgtgcacgc
     4501 gcaggcgttg ttcgcgcagc acgcgggcga tgtcggccag caggccggga cggtcgttgc
     4561 agaccaggcc gatgcgggta ccgccggcga tctcggtgaa gtccagttgg ggcgcgatgc
     4621 ggaaatggcg caggtggcgc ggctggctgc gccgcgccgg ctgcaggcag gccaggtcgc
     4681 cggacagcgc cttggccagt tgcgcttcca cctgcgtcga taccggcgcg atgcgcgtgt
     4741 ccatcggctg cacgtggaac gtgtcgaaga tggtgccgtc ggggccgtcg agcacgcgcg
     4801 cctgcaacac gcccaggccc atccggtcca gcgcgatcac cagcgcggcg aacaggccat
     4861 cgcggtcgcg catgtgcacg aagacctcca gtccctcgcg gccggtcgga tagcgcacgc
     4921 cgaccaccgg acggtcgccc ggcgcggcgt gcatcacccg cacctgccag gcgatctggt
     4981 ccggccggct gcgcaggaag ttgtcgcgcg gcatgcgtgc gaacacctgt tccatcgagg
     5041 catcgtcgtc gccgctcgcc gacagcagcg cgcgcgccgc ggcacggttc tcctcgatgc
     5101 gctgctcggc cgcgaccgga tgctccagcc cacgacgcag ggccaggcgg gtggcggtgt
     5161 gcaggtcggc cagcaggcga tccttccagt cgttccacaa cttggggctg gtgccggcga
     5221 tgtcggcgca ggtcagcagg tacaggtaat ccagtcgctg gcggtcggcg acttcgccgg
     5281 cgaagcggtg gatcacctcg ggatcggtga tgtcctgctt ttgcgcggtc accgacatca
     5341 gcaggtgcct gcgcaccagc cattcgacct gcgcggtgtc ggcctcggac aggtcgtgcg
     5401 cctcgcagaa agcgcgcgca tcgaccgcgc cgagttcgga atgatcgcca ccgcggccct
     5461 tgccgatgtc atggaacagc ccggccagca acaggagctc cggcttgcgc agcgacggcc
     5521 acacgcgatg cgcgatcgag aagcgcgggt cgccctgccc cgacgcgaac gcagcgaggt
     5581 tcttcagcac cgccagcgtg tgctggtcga cggtgtacac atggaacagg tcgaactgca
     5641 tgcgcccgga cacctctgcg aaggccggga tccatcgacc cagcacgccc aggcgggcca
     5701 tgcgctccag caccttcacc ggctgcgggc cgcgcagcag ggccatgaag cgcgcgcgca
     5761 ccgtcggctc gacatcggtg tatgccggga tgccgggcaa ggcctgcgcc agttcgcgcg
     5821 cggtctgcga gtgcaggccg cgcaggccgg tgtccgcagc ccaggtggcg aacaacgcga
     5881 agacattggc agggtcgcgc agcaaccagc ccggcacccg cgtggccagg taaccgcgac
     5941 ggcgctcgaa atcgccgtcg atcggttccg ccagcgcgct gccgtcgaag tgttcctcga
     6001 agcgctgcag caggcggtca ttgatccggc gcacgatcgc cgcgctgcgg tagaagccct
     6061 gcatcatctt ctcgaccgcg agcgagtcgc cgtcgtcttc gtagcccaat cgcgcggcca
     6121 gcagcttctg gtagtcgaag cgcaggcgtt cttcgcggcg accggcgacc aggtgcaggc
     6181 cgtaacgcag gcggcacagg acgcgccgtt cgcgctccag cgcgatcgct tcgtccggcc
     6241 cgaggtggcc caggcccacc agcgcgtcga ggtcgcgcac gccgaagctg cgcagggcca
     6301 gccagcccag catgtgcagg tcgcgcaacc cgcccggccc gtccttgatg ttcggctcca
     6361 ggttgtccga ggtatcgccg aagcgcgcgt ggcgctggcg ctgctcggcg accttggcct
     6421 ccaggaacgc gcgcggcgcc catgcgcggt ccggcgcgat cgtcgcgcgc aaggccgcgg
     6481 tttcctcttc ccaggcgacc agcgggcgcg cttcgatcag cgcggtcagc acggtctggt
     6541 cggccgcggc ctcggtgcat tcggcggcag agcgcaccgc cgggctggcc tgcaggccgg
     6601 catcccagag cagggcgacg aaacgcgcca gcgcgggttg cgcggcgcgc tgcacgtcgg
     6661 gttcggccag cacgagcagg tcgatgtcgg attgcgggaa cagttcgccg cgcccgtagc
     6721 cgccgaccgc gaacagggcc agcgcgtcgc cttccgggat gcagcgctgc caggcatcgc
     6781 ggaccagggt atcgactgcg cgggcgcgca gcgcgaccag cttgtcggcc ggctcaccgg
     6841 cgtcgaagcg gcgggcgaag cgggcgtcgg actgcaccag ccaggcgcgc gccgccgcgg
     6901 cccatgccgg gtcggcatcg accgcaggcg ggatcgcggc gggatcggga agcgtcgccg
     6961 ctggcaggtc ggcggcgggc gtgctcacag gtcgttgtcg tcgccgggca ggcgggtcag
     7021 gatctccacg ccatcggcgg tgaccgccac ggtgtgctcc cactgcgcgg acaacttgcg
     7081 atccttggtg accacggtcc agccgtccgg caacacgcgg gtatggcggc tgccttcgtt
     7141 gatcatcggc tcgatggtga aggtcatccc ttccttcagc accacgccct gccccggctg
     7201 gccgtagtgc agcacctgcg ggtcgtcgtg gtagatcttg ccgatgccgt ggccgcagta
     7261 ctcgcggacc acgctgaagc gctggctttc ggcatattgc tggatcgcat ggccgacatc
     7321 gcccagggtc gcgcccggct tgaccgcgcg gatgccgcgg aacatggcct cgcgggtgac
     7381 atcgaccagg cgctgcgcca tcaccgacgg ggtgccgacg atgtacatcc ggctggtgtc
     7441 gccgtgccag ccatccttga tcacggtgac gtcgatgttg acgatgtcgc catccttgag
     7501 gaccttggcc tcgctgggga tgccgtggca gatcacgttg ttgaccgagg tgcacacggt
     7561 cttcgggaac ccgcggtagc cgacgttggc cgggatcgcc ttctgcacct ggacgatgtg
     7621 atcgtggcag atccggtcga gttcctcggt ggtcaccccg ggtttcacga acggcgcgac
     7681 gatctgcagc acttcggcgg ccaggcggcc tgcctcgcgc atcttgtcga ggtcggcggc
     7741 ggatttcagt tggatgctca tgggccgatt atcgcccatc cggggcggaa ttcggcctgg
     7801 gatgggcctg cggattgcct tggcggggtt atcaggccat aatcccgcgg cttttcccgc
     7861 cacgacgggc gtaccgtcga acctcgcttc aagaatccac acctgtcgac aacccctgcg
     7921 ccggggtgct ccgcaaggag ttcggacgca gtgacgacag ggaggcccaa ccccgggatc
     7981 ccgcgcccca tgccagccgg catgcggtgc agcgccatca tccaatcggc gcggatccca
     8041 tcatcggagt tcctgcaatg ccccagatca ccatgcgcca gatgctggaa gccggcgtcc
     8101 atttcggcca ccagacccgc tactggaacc ccaagatggc accgtacatc ttcggcgccc
     8161 gcggcaagat ccacatcatc aacctcgaga agaccgtccc gctgttcaac gacgcgatga
     8221 acttcatcag cggcgtggcc cagaagcgcg gcaccgtcct gttcatcggc accaagcgca
     8281 gcgcgcgcga cccgatcaag gaagaagccg agcgttgcgg catgccgtat atgacccagc
     8341 gctggctggg cggcaccctg accaacttcc agaccgtgaa gaagtcggtc tcgcgcctga
     8401 aggaactgga agccgcggaa accgacggca ccttccagaa gctggtcaag cacgaagtga
     8461 tgggcctgcg ccgcgagcgc gagaagctcc aggccagcct gggcggcatc aaggacatga
     8521 accgcctgcc ggacgccctg ttcgtcatcg acatcggcca tgaagacatc gccatcaagg
     8581 aagccaagaa gctcggcatc ccggtgatcg cggtggtcga ctccaactac gacccggccc
     8641 tggtcgacta cgccatcccg ggcaacgacg acgccatccg cgccgtgcag ctgtacgcac
     8701 gcgccgccgc cgactcggtg ctggaaggca aggccgccac cccgtccgcc gcgacgatcc
     8761 gcgaggaaga cttcgccgcc gcgccggaag gcgaggacaa gaagccggcc cgccgcgcgc
     8821 cgaagccgaa ggccgagtaa gccccaccga cgccggtcat gccggcgtca cctggccgcg
     8881 ccacgctgcg cggccaccgc tctgctcgtc atcccggcga cagccgggat ccagcattcg
     8941 cgggatcacc gcaagagacc tggattccgg cactcgccgg aacgacgcca tcccacattc
     9001 agaggtaacc ccatggctga tatttccgct tccctggtca aggaactgcg cgagcgcacc
     9061 ggcgccggca tgatggagtg caagaaggcg ctcaccgaga acaacggcga catcgacgct
     9121 tccgccgagt ggctgcgcaa gtcgggcctg gccaaggccg acaagaaggc gagccgcgtg
     9181 gccgccgaag gccgcatcgc ggtggcccag tccgccggca aggcggtgct ggtcgagatc
     9241 aactccgaga ccgacttcgt cgccaaggac gacaacttcg tggccttcac cgacgccgtc
     9301 gcccaggccg cgctggactc cggcgcgacc gacgtggaag cgctgaagtc ggccaagctc
     9361 gcctcgggcg agaccgtcga ggaagcccgc gccgctgcca tcgccaagct cggcgagaac
     9421 atcctggtcc gccgcatcgc gtcgatcacc accggcaaca acgtcgccgc gtactcgcac
     9481 ggcggcaaga tcggcgtgct ggtcgaggtc gccggtggcg acgtggactt cgcgcgcggc
     9541 atcgccatgc acgtggccgc gatgaacccg ccgtacaaca aggccgccga cgtgccggcc
     9601 gacttcgtgg cgaaggaaaa ggaaatcgaa ctggccaaga tgtccgacaa ggacaagcag
     9661 aagccggccg agatcctcga gaagatcatc ggcggcaaga tcgccaagat cgtcaacgaa
     9721 gtcagcctgt acggccagcc gtacgtgctg aacaccgacc acagcgtcga gcaggccgcc
     9781 aaggccgccg gtgccgacgt ggtgtccttc acccgcctcg ccgtgggcga aggcatcgag
     9841 aaggtcgtcg aggactacgc cgcggaagtg gcgaaggcca tgcaggtctg atcgacc
//
LOCUS       DAOOCX010000089         9202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_7114_length_9202_cov_8.379687, whole genome shotgun sequence.
ACCESSION   DAOOCX010000089 DAOOCX010000000
VERSION     DAOOCX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9202
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7114_length_9202_cov_8.379687"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PK399_08850"
     CDS             complement(<1..471)
                     /locus_tag="PK399_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926900.1"
                     /note="catalyzes the hydrolysis of dipeptides with a
                     proline at the C-terminal; also catalyzes the low
                     efficiency hydrolysis of organophosphate di- and
                     tri-esters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HPW13265.1"
                     /translation="MPTTTPAALYPQHLAELQARAAEALRRGGFDHLVVPSGTLHYQL
                     FDDRDYPYAVNPQFKAWLPLTRAPGSWLVITPGMRPTLLFLQPHDYWHVVPAAPSGYW
                     VEHFDIVTIRTPEEAARHLPKDAARCAILGEPQSAVGDYLPNNPPPVVEYLEYHR"
     gene            complement(548..895)
                     /locus_tag="PK399_08855"
     CDS             complement(548..895)
                     /locus_tag="PK399_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPW13266.1"
                     /translation="MGIESRRQARRTVPGMMEVYDTITEEPVGYVGNVSVGGMLLIAH
                     RTLVDDALYQFRFALPDDMGETQPLEVGAHVLWVDDASAPGQSWAGLRFLGLSPEATR
                     RLRWWTEQESHAT"
     gene            complement(885..3260)
                     /locus_tag="PK399_08860"
     CDS             complement(885..3260)
                     /locus_tag="PK399_08860"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HPW13267.1"
                     /translation="MAAAPAPQGSTTARTLALAELPRRVRLVLEHVLQEIRSDMGRQL
                     QTVLLETELALSRQGSPVNDPKVEAAKFVSLNNLRVGQQRFTHRFLADIEAGLAGLHE
                     QRRNRRLEEQQETGQGLTLLDDEAMSDQAMLNNIASRIESRNSLALQLMGQRFGVLAG
                     APAFDGEHLPIGPYALCQALHDASSELEFPPHAKFELYRQFEKVLMALYSTLLEALNN
                     KLASDGILPHLSFVPVRARPASGGSGSPRTTTTMPALGAGDGGPAPASKGAAAIDLPS
                     ALTMEGKGRANFMPAGTQAVPQAPHPVQGSQAAQGPASVAPPKPGGLGAGFAALQNLL
                     KRRRVLLAKLRPGGQDERVREPLRRDEVLGALQRMRANATKADTIGDFRQILLAQARQ
                     MHGHGVALADSDSDTFELLALFLAQLQREMRKGSDGEVLVERLRLPMLQLALRDHRFF
                     VDPSHPGRMLLDAVSRGGARWLAEDDVDLQWLGLLQRAVATVQQDAEGAFDTFVEANQ
                     TLQSGLQALARKTEMAERRQVEAARGREKLEVAKQRANSEIARLLGGRSLPRFHAILM
                     DQAWADVLSLTHLRNGEQSAAWRDLIDTTAAIIDASTSSGQPHADPAFVARVQGALEQ
                     VGYHADDATAIARQLANGRAEDADLASRTELLVQLRARARLGEGNVAAPGTDLPTRDA
                     AEEAAFRQLRALQEPTWVEFDEDADEAIVRRRLAWTSQHTHQTLVVNRRGMRTLSEDL
                     DVLARKLAAGKLRVLEVDAGPAEAAWEATMASLQRIAESEGQQAREDGHGD"
     gene            complement(3342..4508)
                     /gene="hemW"
                     /locus_tag="PK399_08865"
     CDS             complement(3342..4508)
                     /gene="hemW"
                     /locus_tag="PK399_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPW13268.1"
                     /translation="MLTTPPLSLYVHLPWCVRKCPYCDFNSHEFRGEGGRGALPFDAY
                     VDALVADLDFDLPLAWGRTVQTVFFGGGTPSLFPPEAIDRFLQLASARLRFAPNAEIT
                     LETNPGTVEHGPFAGYRAAGVNRLSFGVQSFDDGCLQRLGRIHSSGDAERAVKAAQDA
                     GIDNLNIDLMYALPGQTLAMARLDVERALALQPTHMSHYQLTLEPNTVFAARPPAGIP
                     DEDSAWDMQDDCQALLAASGFAQYEVSAYARDGHQCVHNLNYWRFGDYLGIGAGAHGK
                     LTLGASQQVLRRWKHKHPSLYLASAGSEAAIGGDEVLGAGRLPFDFMLNALRLNDGVP
                     MAMFEARTGLPRSAIAVPVARARAQGWLDADADWLRPTELGRRFTNDVIGLFLE"
     gene            complement(4557..5147)
                     /gene="rdgB"
                     /locus_tag="PK399_08870"
     CDS             complement(4557..5147)
                     /gene="rdgB"
                     /locus_tag="PK399_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPW13269.1"
                     /translation="MKLVLASSNAGKLVELRDLLGDTGIELHAQSEFGVGDAEETGLS
                     FIENAILKARHAARATGLAALGDDSGLCVDALDGAPGLYSARYSGVHGNSEANIDKLL
                     GHMQGIADDARTARFVCVLALVRHANDPMPLVAEGVWEGHILHARTGSNGFGYDPVFF
                     SPAHGCGAAELPSETKNRDSHRGIALAMLRERLGRP"
     gene            complement(5144..5872)
                     /gene="rph"
                     /locus_tag="PK399_08875"
     CDS             complement(5144..5872)
                     /gene="rph"
                     /locus_tag="PK399_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPW13270.1"
                     /translation="MSFTRPSGRSPDQLRSVSITRGFTRHAEGSVLVAFGDTRVLCTA
                     SVENKVPGFMRGKGEGWITAEYGMLPRATHTRNDREASRGKQGGRTLEIQRLIGRSLR
                     ACVDRAALGERTITLDCDVLQADGGTRTAAITGAYVALVDAVRWMQAKRDITKNPIIG
                     AVAAVSVGIYRGTPVLDLDYAEDSDCDTDMNVVMNDGGGFIELQGTAEGHAFRRDELD
                     ALLVLAGQGCNELFAAQQAALAAP"
     gene            5967..6815
                     /locus_tag="PK399_08880"
     CDS             5967..6815
                     /locus_tag="PK399_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPW13271.1"
                     /translation="MTAYAAGERSTEWGLLGCEMRAVNHRFLELGVRLPDELRVLEPQ
                     LRERVATRVSRGKLDLVLRLRSANGSDALQVDEALVAKLGDLARQLDAGFPQLRVQFT
                     DLLQFPGVLQARGVDVDALQREAMVLLDAVLDDFVASREREGGKLAEVIGERATAIAA
                     IAAEVRTLVPLIRTGQRTKLEARLADLSQPADPGRVEQELVLWLQKLDVDEELDRLDS
                     HLSELRRTLKQKEPVGRRLDFLLQEFNREANTLGSKSVDARTSNAAVELKVLIDQIRE
                     QIQNIE"
     gene            6833..7450
                     /gene="gmk"
                     /locus_tag="PK399_08885"
     CDS             6833..7450
                     /gene="gmk"
                     /locus_tag="PK399_08885"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPW13272.1"
                     /translation="MRGTLYIVAAPSGAGKSSIVNAVLARDANISLSISFTSRQPRPG
                     ERHAEHYHFIGADEFKAMVAEGEFFEHALVHGDWKGTARQSVDPQLSAGRDVLLEIDW
                     QGARQVRAKIPDAVSVFILPPSRAALEERMRKRGQDSEDVIRQRLDAAREEMSHYGEF
                     DYVIVNEDFETAVTEMCSIFTASRLRKDAQVARHSRLITSLLVDD"
     gene            7579..7878
                     /gene="rpoZ"
                     /locus_tag="PK399_08890"
     CDS             7579..7878
                     /gene="rpoZ"
                     /locus_tag="PK399_08890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPW13273.1"
                     /translation="MARITVEDCLEVVDNRFDLVMMAAKRARQLAGGVEPKLDNSEAN
                     DKPTVLALREIAARQIDTDYINAVEKAERERKEREALEWAAAEVVAGDDDMKGDD"
     gene            7980..>9202
                     /locus_tag="PK399_08895"
     CDS             7980..>9202
                     /locus_tag="PK399_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011269543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPW13274.1"
                     /translation="MSPGDPALALPLPAPDQGAVPDYVQSLERAASYLPDAQRAQLRR
                     AWAVGAAAHAGQTRKSGEPYITHPVAVAQVLAEQGLDMETLVAAILHDTIEDTPLSRQ
                     DLATQFGETVAELVDGVTKLDKLRFADRQEAAAESFRKMLLAMSRDLRVILIKLADRL
                     HNMRTLGAQSAEARRRIAVETLEIFAPIAQRLGMNLIKSELQDLGFRALHPWRHAVIE
                     KRIRSQPVVRREALAQIEARLAQKLALEKLPHRLVGRIKTPWSIYTKMHAENRSFDQV
                     MDVFGYRVIVDNVPDCYHALGVVHSVYKPLDARFRDFIAIPKANGYQSLHTVLFGPYG
                     SPIEVQIRTREMDLVAERGVAAHWAYKHGGDAGNSAQSRASAWIAGLVESQRGTGSSL
                     EFLENVKVDLFPDEV"
BASE COUNT         1404 a         3111 c         3207 g         1480 t
ORIGIN      
        1 gcggtggtac tccaggtact cgaccaccgg cggcgggttg ttcggcaggt agtcgccgac
       61 cgcactttgc ggctcaccca ggatcgcgca tcgggcggcg tccttcggca ggtggcgcgc
      121 ggcttcctcc ggcgtgcgga tggtgacgat gtcgaaatgc tcgacccagt agccgctggg
      181 cgcggccggc accacgtgcc agtaatcgtg cggttgcagg aacagcagcg tcgggcgcat
      241 gcccggggtg atcaccagcc agctgccggg cgcgcgggtc agcggcagcc aggccttgaa
      301 ctgcggattc accgcgtacg ggtagtcgcg gtcgtcgaag agctggtagt gcaaggttcc
      361 gctgggcacg accaggtggt cgaagccgcc gcggcggagc gcttcggcgg cgcgcgcctg
      421 caattcggcc aggtgctgcg ggtacagggc ggcgggcgtg gtcgttggca tggcgggcgt
      481 ctcgcggggc ggaacgtcga ttttgaccga tcccgccggc tcgcacagtg cgtgaagtcc
      541 cgccggctca ggtcgcgtgc gattcctgct cggtccacca gcgcaggcgg cgggtcgcct
      601 ccggcgacag gccgaggaaa cgcagcccgg cccaggattg ccccggggca ctggcgtcgt
      661 cgacccagag cacgtgcgcg ccgacttcca gcggctgggt ttcgcccatg tcgtccggca
      721 gggcgaagcg gaactggtag agcgcatcgt cgaccagcgt gcggtgggcg atcagcagca
      781 tgccccccac cgagacattg cccacgtagc caaccggctc ctcggtgatg gtgtcgtaga
      841 cctccatcat tcccggcacg gtgcggcgtg cctggcggcg cgattcaatc cccatggccg
      901 tcctcccggg cctgctggcc ctcgctttcg gcgatccgtt gcaggctggc catggtcgct
      961 tcccacgcgg cttccgccgg tccggcatcg acctccagca cgcgcagctt gccggccgcc
     1021 agcttgcgcg ccagcacgtc gaggtcctcg ctcagcgtgc gcatgccacg gcggttgacc
     1081 accagggtct ggtgggtgtg ctggctggtc caggccagcc tgcgccggac gatggcctcg
     1141 tcggcgtcct cgtcgaactc cacccaggtc ggttcctgca gcgcacgcag ctgcctgaag
     1201 gccgcttctt ccgctgcgtc gcgggtcggc aggtcggtgc ccggcgcggc cacgttgcct
     1261 tcgcccaggc gcgcgcgcgc gcgcagttgc accagcaatt cggtgcgcga ggccaggtcg
     1321 gcatcctccg cgcggccatt cgccagttgg cgggcgatcg cggtggcgtc gtcggcgtga
     1381 tagccgacct gctccagggc gccctgcacg cgtgccacga aggccgggtc cgcgtgcggt
     1441 tggccactgc tggtgctggc atcgatgatc gcggcggtgg tgtcgatcag gtcccgccag
     1501 gcggcggact gttcgccgtt gcgcaggtgg gtcagcgaca acacgtccgc ccacgcctgg
     1561 tccatcagga tggcgtggaa gcggggcagg ctgcgcccgc ccagcaggcg tgcgatctcg
     1621 ctgttggcgc gctgcttggc gacctccagt ttctcgcggc cgcgcgcggc ttccacctgc
     1681 ctgcgctcgg ccatctcggt cttgcgggcc agcgcctgca gtccggattg cagggtctgg
     1741 ttggcttcga cgaaggtgtc gaaggcgcct tccgcatcct gctgcacggt ggccaccgcg
     1801 cgctgcagca agccgagcca ttgcaggtcg acgtcgtcct cggccagcca gcgcgcacca
     1861 ccgcgcgaca ccgcatccag cagcatccgc ccgggatggc tggggtcgac gaagaagcgg
     1921 tggtcgcgca aggcgagctg cagcatcggc aggcgcaggc gttcgaccag tacctcgccg
     1981 tcgctgccct tgcgcatctc gcgttgcagc tgggccagga acagggccag caactcgaag
     2041 gtgtcgctgt ccgaatcggc cagggcgacc ccgtggccgt gcatctgccg cgcctgcgcc
     2101 agcaggatct gccggaaatc gccgatggtg tcggccttgg tcgcgttggc gcgcatccgc
     2161 tgcagcgccc ccagcacttc gtcgcgccgc agcggttcgc gcacgcgctc gtcctggcca
     2221 ccggggcgca gcttggccaa cagcacgcgg cggcgcttga gcaggttctg cagcgcggcg
     2281 aagccggcgc ccaggccgcc cggtttcggc ggcgccacac tggccggccc ctgggctgcc
     2341 tgcgacccct gcaccgggtg gggcgcctgc gggacggcct gcgtgcccgc aggcatgaag
     2401 ttggcccggc ccttgccttc catcgtgagc gcagatggca ggtcgatggc agcagccccc
     2461 ttggaagcgg gcgccggacc gccgtcgccg gcacccagcg cgggcatcgt cgtggtggtg
     2521 cggggcgagc ccgaaccgcc cgacgctggc cgtgcccgca ccggcacgaa gctcaggtgc
     2581 ggcaggatgc cgtcgctggc cagcttgttg ttcaaggcct ccagcaaggt cgagtacagc
     2641 gccatcagca ctttctcgaa ttgccggtac agctcgaact tggcgtgcgg ggggaattcg
     2701 agctcgctgc tcgcgtcgtg cagcgcctgg cacagcgcat acggtccgat cggcaggtgt
     2761 tcgccgtcga aggccggcgc accggccagc accccgaacc gctggcccat cagttgcagg
     2821 gcgaggctgt tgcgcgattc gatccggctg gcgatgttgt tgagcatcgc ctggtcgctc
     2881 atcgcttcgt cgtcgagcag ggtcaggccc tggccggttt cctgctgttc ctcgaggcgc
     2941 cgattgcggc gctgttcgtg caggccggcc aggccggctt cgatgtcggc caggaagcgg
     3001 tgggtgaaac gttgctggcc gacccgcagg ttgttcagcg acacgaattt cgccgcttcc
     3061 acctttggat cgttgaccgg gctgccctgg cgggacagcg ccagttcggt ttccagcagg
     3121 acggtctgca attgccggcc catgtcgctg cggatttcct gcaggacatg ttccagcacc
     3181 aggcgcacgc ggcgcggaag ctcggcgagc gctagcgtcc tggcggtggt cgatccctgt
     3241 ggagcgggtg ctgcggccat cggcgtcccc ctacgcgtcg gcgaacgctc gatggtatgc
     3301 gttgcgcagt cggctctcaa ctggaaccgg cgtcacggat ttcattccag gaacaggcca
     3361 atgacgtcgt tggtgaaccg gcgtccgagt tcggtcgggc gcagccagtc ggcgtcggcg
     3421 tcaagccacc cctgcgcgcg tgcacgcgcc accgggacgg cgatcgcact gcgcggcagg
     3481 ccggtgcgcg cctcgaacat cgccatcggc acgccgtcgt tgaggcgcag cgcgttcaac
     3541 atgaagtcga agggcaggcg gccagcaccc aggacttcgt cgccaccgat cgccgcttcg
     3601 ctgccggcgc tggccaggta cagcgacgga tgcttgtgct tccagcggcg cagcacctgc
     3661 tgcgatgcgc ccagggtcag cttgccgtgc gcgccggcac cgatgcccag gtagtcgccg
     3721 aagcgccagt aattcaggtt gtgcacgcat tggtggccgt cgcgggcata ggcgctgact
     3781 tcgtactggg cgaaaccgga ggccgccagc agcgcctggc agtcgtcctg catgtcccag
     3841 gcgctgtcct cgtcgggaat gcccgcgggc gggcgggccg cgaacacggt gttcggctcc
     3901 agggtcagct ggtagtggct catgtgcgtg ggttgcagcg ccaacgcgcg ttccacgtcc
     3961 agccgcgcca tcgccagtgt ctgccccggc agcgcataca tcaggtcgat gttgaggttg
     4021 tcgatgccgg catcctgcgc ggccttgacc gcgcgttccg cgtcgccgct ggaatggatc
     4081 ctgccaagcc gctgcaggca gccgtcatcg aagctctgca cgccgaagct caggcgattc
     4141 accccggccg cgcgataacc ggcgaacggg ccgtgctcga ccgtgcccgg gtttgtctcc
     4201 agggtgatct cggcgttcgg cgcgaagcgc aggcgcgcgc tggccagttg caggaagcgg
     4261 tcgatcgcct ccggcgggaa caggctgggc gtgccgccgc cgaagaacac cgtctgcacg
     4321 gtgcggcccc aggccagcgg caggtcgaag tcgaggtcgg cgaccagcgc atcgacatag
     4381 gcatcgaagg gcagcgcgcc gcgcccgccc tcgccgcgga attcgtgcga attgaaatcg
     4441 cagtacgggc atttgcgcac gcaccagggc aggtgcacgt aaagcgaaag cggcggggtg
     4501 gtcagcatgg atgcttgctc ttgctcgtca tccccgcctt cgcgggaatg acggcatcaa
     4561 ggtctcccca gccgttcccg caacatcgcc aacgcgatcc cgcggtggct gtcgcgattc
     4621 ttcgtttcgg acggcagttc ggccgcgccg caaccatgcg ccggcgagaa gaacaccggg
     4681 tcgtagccga agccgttgct gccggtgcgc gcgtgcagga tgtggccttc ccagacgccc
     4741 tcggcaacca gcggcatcgg gtcgttcgca tgccgcacca gcgcgagcac gcagacgaag
     4801 cgcgccgtgc gcgcgtcgtc ggcgatgccc tgcatgtggc ccagcagctt gtcgatgttc
     4861 gcttcgctgt tgccatgcac gcccgagtag cgcgccgaat acaggccggg cgcaccatcg
     4921 agcgcatcga cgcacaggcc cgagtcatcg cccagcgcag ccaggccggt cgcgcgcgcc
     4981 gcatggcgtg ccttgaggat cgcgttctcg atgaagctca ggccggtttc ctcggcatcg
     5041 cccacgccga actcggattg cgcgtgcagc tcgataccgg tatcgcccag caggtcgcgg
     5101 agttcgacca gcttgccggc gttggaactg gccaggacga gtttcaaggc gcggcgagcg
     5161 ccgcctgttg cgcggcgaac agctcgttgc acccttggcc ggcgagcacc agcagcgcgt
     5221 ccagttcatc gcggcggaag gcgtggcctt cggcggtgcc ctgcagttcg atgaagccac
     5281 cgccgtcgtt catcaccacg ttcatgtcag tgtcgcagtc gctgtcctcg gcatagtcga
     5341 ggtcgagcac cggcgtgccg cgatagatgc ccaccgacac tgccgcgacc gcgccgatga
     5401 tcgggttctt ggtgatgtcg cgcttcgcct gcatccagcg caccgcatcc accagcgcga
     5461 cgtacgcgcc tgtgatcgcg gcggtacggg tgccgccatc cgcctgcagc acgtcgcagt
     5521 cgagggtgat cgtgcgttcg cccagcgccg cgcgatccac gcaggcgcgc aggctgcgcc
     5581 cgatcaggcg ctggatttcg agcgtgcgcc cgccctgctt gccgcgcgag gcttcgcggt
     5641 cgttgcgggt atgggtggcg cgcggaagca tgccgtactc ggcggtgatc cagccttcgc
     5701 ccttgccgcg catgaagccg ggcaccttgt tctcgacgct ggcggtgcac agcacgcggg
     5761 tgtcgccgaa cgccaccagc accgagccct cggcatggcg ggtgaagccg cgggtgatcg
     5821 acacggagcg caactggtcg ggactgcggc cgctgggacg ggtgaaggac atcgggttcc
     5881 ggcgaggaat gccggcagcc ggcggggcgc tagattacca tccaccttcc gctctcccgg
     5941 ccgcccgcac gatgcccatc cgcagcatga ccgcctacgc cgccggcgag cgcagcaccg
     6001 aatggggctt gctcggctgc gaaatgcgcg cggtcaacca tcgattcctc gagctcggcg
     6061 tgcgcctgcc ggatgaattg cgcgtgcttg agccgcagtt gcgtgagcgc gtggcgacgc
     6121 gggtgtcgcg cggcaagctg gacctggtgc tgcggctgcg ctcggccaac ggcagcgacg
     6181 cgctgcaggt ggacgaggcg ctggtggcga agctgggcga cctggcacga caactggatg
     6241 cagggttccc gcaactgcgc gtgcagttca ccgacctgct gcagttccct ggcgtgttgc
     6301 aggcgcgtgg cgtcgatgtc gatgccttgc agcgcgaggc gatggtgttg ctcgatgccg
     6361 tcttggacga cttcgtcgcc tcgcgcgagc gcgaaggcgg caagttggcc gaggtcatcg
     6421 gcgagcgcgc gaccgcgatc gccgcgatcg ccgccgaggt gcgcacgctg gtgccgctga
     6481 tccgcaccgg ccagcgcacc aagcttgagg cgcgcctggc cgacctctcg cagcccgccg
     6541 atccgggccg tgtcgagcag gaactggtgt tgtggctgca gaaactcgat gtcgatgaag
     6601 agctcgaccg cctcgacagc catctgtccg aactgcgccg gaccctgaag cagaaggagc
     6661 cggtgggtcg ccgcctggac ttcctgctgc aggaattcaa ccgcgaggcc aataccctgg
     6721 gcagcaagtc ggtcgacgcg cgcaccagca acgccgcggt ggagctgaag gtgctgatcg
     6781 accagatccg cgaacagatc cagaacatcg aatgaaagca gtcgagtaag ccatgcgcgg
     6841 aaccctctac atcgtcgccg cgccctccgg tgccgggaaa tcgagcatcg tcaacgcggt
     6901 gctggcgcgc gatgcgaaca tcagcctgtc gatctcgttc acctcgcggc aaccgcgccc
     6961 gggcgaacgg catgccgagc attaccactt catcggcgcc gacgagttca aggcgatggt
     7021 cgccgagggc gagttcttcg agcacgcgct ggtgcacggg gactggaagg gcaccgcgcg
     7081 gcaatcggtg gatccgcagc tgtccgccgg tcgcgacgtg ttgctggaaa tcgactggca
     7141 gggcgcgcgc caggtgcgcg ccaagatccc cgacgcggtc agcgtgttca tcctgccgcc
     7201 atcgcgtgcc gcgctggaag aacgcatgcg caagcgcggg caggattcgg aagacgtcat
     7261 ccgccagcgg ctggacgccg cccgcgagga aatgagccac tacggcgaat tcgactacgt
     7321 gatcgtcaac gaggatttcg agaccgcggt gaccgagatg tgcagcatct tcaccgccag
     7381 ccgcctgcgc aaggatgccc aggtggcgcg tcattcccgc ctgatcacct ccctgctggt
     7441 cgacgactag cgtgccgcgg gtgccgctgg atgcctaagt tcatgatttc caaggcatag
     7501 cttgccttca tgccgctcgc cagctagaat tccggcccct ctcgaacact tcagcggccc
     7561 agcggtcgca ggacatacat ggcccgtatt accgtggaag attgcctgga agtggtcgac
     7621 aaccgcttcg acctcgtgat gatggccgcc aagcgcgcgc gccagctggc gggcggcgtg
     7681 gagccgaagc tggacaacag cgaagccaac gacaagccga ccgtcctggc cctgcgcgag
     7741 atcgccgcgc gccagatcga caccgactac atcaatgcgg tggagaaggc cgagcgcgag
     7801 cgcaaggagc gcgaggcgct ggagtgggcc gccgcggaag tggtggcggg cgacgacgac
     7861 atgaagggcg acgactgatc gttcgcttcc atgacccggg aaaaacggcc ctgcggggcc
     7921 gtttttcgtt tccgggacag gggtttacgg ctcccatacg gcccggttag gctcgctgca
     7981 tgagtcccgg cgatcctgcg ctagccctgc cgttgcccgc acccgatcaa ggtgcggtcc
     8041 ccgactacgt gcaatcgctg gaacgcgccg cgagctacct gccggatgcg cagcgtgcgc
     8101 agctgcggcg ggcgtgggcg gtgggcgcgg ccgcgcacgc cgggcagacc cgcaaatcgg
     8161 gcgagcccta catcacccat ccggtcgcgg tggcccaggt gctggccgag cagggcctgg
     8221 acatggaaac gctcgtcgcc gcgatcctgc acgacaccat cgaggacacg ccgctctcgc
     8281 gccaggacct ggccacgcag ttcggcgaga ccgtggccga actggtcgat ggcgtcacca
     8341 agctggacaa gctgcgcttc gccgatcgcc aggaagccgc cgcggagagt ttccgcaaga
     8401 tgctgctggc gatgtcgcgc gacctgcggg tcatcctgat caagctggcc gaccgcctgc
     8461 acaacatgcg cacgctgggc gcgcagtccg cggaagcgcg ccggcgcatc gcggtggaaa
     8521 cgctggagat cttcgcgccc atcgcccagc ggctgggcat gaacctgatc aagtcggagc
     8581 tgcaggacct cggtttccgc gcgctgcatc cgtggcggca tgcggtgatc gagaaacgca
     8641 tccgcagcca gccggtggtg cgtcgcgagg cgctggcgca gatcgaggcg cgcctggcgc
     8701 agaaactggc gctggaaaaa ctgccgcacc ggctggtcgg gcggatcaag acgccttgga
     8761 gcatctacac caagatgcac gcggagaacc gcagcttcga ccaggtcatg gacgtgttcg
     8821 gctaccgggt cattgtcgac aacgtgccgg actgctacca cgcgctcggc gtcgtccact
     8881 cggtctacaa gccgctcgac gcacgtttcc gcgacttcat cgcgattccc aaggcgaatg
     8941 gctaccagtc gctgcacacg gtgctgttcg ggccctacgg ttcgccgatc gaagtgcaga
     9001 tccgcacccg cgagatggac ctggtcgctg aacgcggcgt ggccgcgcac tgggcgtaca
     9061 agcacggcgg cgatgccggc aacagcgcgc agagccgcgc ctcggcgtgg atcgccggcc
     9121 tggtcgaaag ccagcgcggg accggctcgt cgctggaatt cctggagaac gtcaaggtcg
     9181 acctgttccc ggacgaggtc ta
//
LOCUS       DAOOCX010000090         9079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_7248_length_9079_cov_5.893285, whole genome shotgun sequence.
ACCESSION   DAOOCX010000090 DAOOCX010000000
VERSION     DAOOCX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9079
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7248_length_9079_cov_5.893285"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PK399_08900"
     CDS             complement(<1..694)
                     /locus_tag="PK399_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW13275.1"
                     /translation="MVDFERLARPGVRELRAYDPGHDLVALRRRFAEAQLVELGSNEN
                     PYGASPDARQAIQKALDGIHIYPDPLGGDLKRALAAHHGVQPANLLLGNGSHELLMQF
                     AQVFAGPGDEVVASKFGFAVYALAAQAAGATLRPAPALPRENAMPRGHDLAGLRASIG
                     PRTKLVYLANPNNPTGTWYAADMFEAFMASVPGDVIVVVDEAYAEFTDAPDYASALPL
                     VTRHPNLVVTRTF"
     gene            complement(702..1760)
                     /gene="pheA"
                     /locus_tag="PK399_08905"
                     /pseudo
     CDS             complement(702..1760)
                     /gene="pheA"
                     /locus_tag="PK399_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300926.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
     gene            complement(1858..2943)
                     /gene="serC"
                     /locus_tag="PK399_08910"
     CDS             complement(1858..2943)
                     /gene="serC"
                     /locus_tag="PK399_08910"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPW13276.1"
                     /translation="MSRAYNFSPGPAALPEPVLRQAQAEMLEYRDSGASIVEISHRGE
                     AFLDVAHRTEADLRSLLSIPDDYAVLFTAGGATTVQALLPLNFANAGQPVDYVVTGHW
                     GKTALKQAAPTADARIAASTEAGGYRSLPPRGEWSLSPDAAYVHVTANETIHGVEFRE
                     IPDVGDVPLIADFSSSIASEPLDISRFGAIYAGAQKNLGPVGIAVLVVRRDLLERAGQ
                     PRAPIFDYRAQLEGESMLNTPPTWNWYMLGLTVKWMLDEGGVAVFAERNARKAALLYA
                     AIDGSGGFYRNEVAREARSRMNVPFFLRDPALDAAFLRESKAAGLVALKGHRVLGGMR
                     ASIYNAMPEAGVQALVDFMQEFQQRHG"
     gene            3116..4081
                     /gene="msrP"
                     /locus_tag="PK399_08915"
     CDS             3116..4081
                     /gene="msrP"
                     /locus_tag="PK399_08915"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HPW13277.1"
                     /translation="MNLRDALRIPSADVTEESVYRDRRRVLAMIGAVPALGLAGCSEA
                     APPASKATITPELAKAGFRTAEEQTREIDATTYNNFYEFGTAKDDPANAPKTLRTAPW
                     TVAVGGQCAKPGKLSLEDLIKGLAPQERIYRMRCVEGWSMVIPWLGVPLGDVLKRFEP
                     TSKAKYVAFTSLADPQQMPGVRARVLDWPYREGLRIDEAMHPLTLLATGMYGKALPQQ
                     NGAPLRLVVPWKYGFKGIKSIIAITFVERMPKTSWNMSQASEYGFFSNVNPAVDHPRW
                     SQASERRIAGSASKLFAERVPTLPFNGYAAQVASLYAGMDLKKWF"
     gene            4114..4749
                     /gene="msrQ"
                     /locus_tag="PK399_08920"
     CDS             4114..4749
                     /gene="msrQ"
                     /locus_tag="PK399_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HPW13278.1"
                     /translation="MVVAKAAVHLAALTPLAILLWQMWGELKGVSHALGADPVAEIEH
                     RLGLWALRFLMLTLAITPLRQLTGWNVLVRFRRMLGLYAFAYASLHFAAYLGLDLRGF
                     WAQVFQDIVKRPYITVGFIAWLLLVPLAVTSTTGWIKRLGRNWARLHKLVYGIAVLAV
                     LHFWWLVKSDLREPALYAGVAALLLGWRAWKALKTRAGARAKARSVPSARA"
     gene            complement(4721..4993)
                     /locus_tag="PK399_08925"
     CDS             complement(4721..4993)
                     /locus_tag="PK399_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HPW13279.1"
                     /translation="MSRIDIRHPHDLPKAKAKKAIDEVAKKLAERFQMECAWDGDVLH
                     FTRSGVEGQIALGKDDLHVHAKLGFLTAMFKDPIENEIKRVLKERF"
     gene            5089..5880
                     /locus_tag="PK399_08930"
     CDS             5089..5880
                     /locus_tag="PK399_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPW13280.1"
                     /translation="MSELRLRFSDGDQPDLVVGAGVHALGRLPTGLGPVDAEQPWLLR
                     VSNDRRGLWLTVAEGLRGVHVNGRPVQHVAMLRAGDSIHVDGGELLLVAANDGRAVAP
                     VIAGRDPVGNLRLVLRGLGGSHHGRSLNLEKPRRIGSAADADLRIDGPGIAPQHAVLE
                     AVNGQAVLRDAAADVQVNGQRVRHALLQAGDQVAFDVQHRFVLEGPPPAAAVPPPRAR
                     DGNGDAADEVPAGGRRHWTLRIPWLLLAAILLSAALSVLLLFGER"
     gene            complement(5888..6631)
                     /locus_tag="PK399_08935"
     CDS             complement(5888..6631)
                     /locus_tag="PK399_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13281.1"
                     /translation="MPARKPARNPVRTDPIARLTRRLAWHQGMYDPEREPRNASRWLK
                     PLQSWQAQRLQRSFAGFLGDPVRRPAAQFFLTDLYGEHDFSQRDADIARVIPMMQKLL
                     PAALLKTVADGIELAVLTHALDLRMVEALQALGGRRRSIDADLYARAYRAVGHRRLRA
                     RQISLIDEVGHGLWSALRMHGVGALLKISRMPARAMGFGELQGFLERGFDAFGGLGDA
                     GPFLADIRANETRVMQRLFAADPDPFRQP"
     gene            6718..7989
                     /locus_tag="PK399_08940"
     CDS             6718..7989
                     /locus_tag="PK399_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPW13282.1"
                     /translation="MLSLHAAQQRHPGRSHAPRIGLAIAGGGPIGAMYELGALRALDE
                     ACEGLDLTRLDCYVGVSSGAFFAAGLANRMDTAELCRIFITGESDDVVFRPETFLRLA
                     TDEYLRRAVAFPKLAARLTRDMLSGRGDSRWSDIVTRIGGLVPTGIFDNAGVEHFLRE
                     VFTRRGRSNDFRKLERPLYVIGVDLDSGEAVRFGDAGWDSVPISRAVQASAALPGLYP
                     PVEIRGRHFVDGALRRTMHASVVLERDVDLMLGINPLVPFNPGTDEYADAQAHRFSDR
                     RIAEGGFPGVISQALRTMLQSRMQVGLARYPAQYPHIDQVVFEPNADNRELFYTNIFS
                     FAARRRISQLAYRNTLADLRKRRDELEPVLARHGIRLRADVIADRPRSIIAHLPAQPR
                     STETTAVLDRALDEIEHRLRTDKRAQARGMV"
     gene            8064..8654
                     /locus_tag="PK399_08945"
     CDS             8064..8654
                     /locus_tag="PK399_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HPW13283.1"
                     /translation="MAKLKKTARKTASGTKQASQDQAERLGKSITESAQQIWLAGMGA
                     FNRAQAEGGKLFESLVRDGLSLEQTARKLAGNRANVVRDVVEGRVGQARERAVDTWDK
                     LEKVFEDRVQRALVKLGVPGRDDLNALTQRVERLTEELRKANGGSAPKAARAPRKAAA
                     PKPAKAPRQAKAPKATKAAKAPARKAAKAAAEAPAS"
     gene            8810..>9079
                     /locus_tag="PK399_08950"
     CDS             8810..>9079
                     /locus_tag="PK399_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13284.1"
                     /translation="MSPILIALLVFVVLGLAATAWLRRPPSPPSTSQANARGAAQLGT
                     LRWRDFTRLVLQAMHGRGYRTVVEDGMPADGIPTDGGDIVLERGGE"
BASE COUNT         1405 a         3147 c         3110 g         1417 t
ORIGIN      
        1 tgaaggtacg ggtaaccacc aggttcgggt ggcgcgtcac cagcggcagc gcggaggcgt
       61 agtccggtgc atcggtgaat tcggcatagg cctcgtccac caccacgatc acgtcgcccg
      121 gcaccgatgc catgaaggcc tcgaacatgt cggcggcgta ccaggtgccg gtcgggttgt
      181 tgggattggc gaggtacacc agcttggtgc gcggcccgat gctggcgcgc aagccggcca
      241 ggtcatggcc gcgcggcatc gcgttttcac gcggcaacgc cggcgcaggt ctcagcgtcg
      301 cacctgccgc ctgcgccgcc agtgcataca ccgcgaagcc gaacttcgac gcgaccacct
      361 catcgccggg gcccgcgaac acctgcgcga actgcatcaa gagctcatgc gagccgttgc
      421 cgagcaacag gttggccggc tgcacgccgt ggtgcgcggc gagcgcacgc ttgaggtcgc
      481 cgccgagcgg atccggatag atgtggatgc cgtcgagcgc cttctggatc gcctgccgcg
      541 cgtccgggct ggcgccgtaa gggttttcgt tggacccgag ctcgaccagt tgcgcctcgg
      601 cgaaacgccg gcgcagcgcg accaggtcgt ggccgggatc gtaggcacgc agttcgcgca
      661 cgccgggccg ggccaaacgt tcgaaatcga ccatgccggc ctcagaggat cgcgacggga
      721 taggagccga gcacgcgcac ttcgtcgccc tgctgggcga tctcgtccag cgcgtctttg
      781 agcggcgatt cgtcgcgatg gccggcgaca tcgatgaaga acgcgtactg ccagaggtgg
      841 ccgtgcgcgg gccgcgattc gatccggttc atgctgatgc cgcgccgcgc cagcggttcc
      901 agcacgccgt agagcgcgcc tggacggtcg cggatgaaca ccagcagcga ggtgcggtcg
      961 tggcccgacg acgggaacga ctcacgcccc agcaccagga agcgggtggt gttgtcgctg
     1021 cggtcctcga tcggcccggc gaccttcttc aggccgtaca catggcccgc cgactcaccg
     1081 gcgatcgccg cggcgtcatc ggcattgcgg gcgcggcgcg cgccttcggc attgctcgaa
     1141 accgggatct tttcgacctt cggcaaatgc gtgcgcaacc aaccgccggt ctgcgcgaac
     1201 gcctgcggat gcgcgtagat gcgttcgata tcctcgatgc ggccgctgcg cgacagcagg
     1261 tgctggtgca cgcgcagctc gacttcgccg cagatccgca acggcgaggt caggaacagg
     1321 tccagcgtca cctggatggt gccctgcccc gagttctcca ctggcaccac gccgaaatcg
     1381 gcgttgccgg cggcgacttc ctggaacact tcctcgatgc tggccagcgg caggccatgc
     1441 gcagaatggc cgaaatgctt gtgcaccgcc tgctggctga acgtgccttc cgggccgagg
     1501 tagccgatct tcaacggctc ctgctgggcc aggcaggccg acatgacttc gcggaacagg
     1561 cggaccagca cgtcgtcggc cagcgggccg tcgttgcgat ccaccacctt gcgcaagact
     1621 tgcgcctcgc gctccgggcg gtagtaatcg actgcggcag cgagcttgcc cttggccttg
     1681 ccgacctggt gcgcccactg cgcgcgttcg gcgatcagtt cctggatgcg tcggtcgatg
     1741 ccgtcgatct gctcgcgcac ggcgcccagg tcgggtttcg actcgccttt ggctgtcgca
     1801 tcgacctgcg gcctggccgg cgttttcgct gcccccggct tgcgggtgtc tttcttctta
     1861 gccatggcgc tgctggaact cctgcatgaa atcgaccaag gcctgcacgc cggcctccgg
     1921 catcgcgttg tagatcgagg cgcgcatccc gccgaggacg cggtggccct tcagcgccac
     1981 caggcccgcg gccttgctct cgcgcaggaa cgcggcgtcg agggcgggat cgcggaggaa
     2041 gaacggcacg ttcatgcgcg aacgcgcctc gcgcgcgact tcgttgcggt agaagccgcc
     2101 ggagccgtcg atcgcggcat acagcaacgc cgccttgcgc gcattgcgtt ccgcgaagac
     2161 ggccacgccg ccttcgtcga gcatccattt cacggtcagg ccgagcatgt accagttcca
     2221 ggtcggcggc gtgttgagca tcgattcgcc ctcgagctgg gcgcggtaat cgaagatcgg
     2281 tgcccgcggc tggcccgcgc gctccagcaa atcgcggcgc acgaccagca cagcgatgcc
     2341 gaccggcccc aggttcttct gcgcgccggc gtagatcgcg ccgaagcgac tgatatccag
     2401 cggttcggac gcgatcgagg aactgaaatc ggcgatcagc ggcacgtcgc caacgtcggg
     2461 gatctcgcgg aactcgacgc catggatggt ttcgttggcg gtgacgtgca cgtaggcggc
     2521 atccggcgag agcgaccact cgccacgcgg cggcaggctg cggtatccgc ctgcctcggt
     2581 gctggcggcg atgcgggcgt cggcggtggg cgcagcctgt ttcagcgcgg tcttgcccca
     2641 gtgcccggtg accacgtaat ccaccggctg cccggcgttg gcgaaattga gcggcaacag
     2701 cgcctgtacg gtggtggcgc cgccggcggt gaacagcacc gcgtagtcgt cggggatcga
     2761 caacaggctg cgcaagtcgg cctcggtgcg atgggcgacg tcgaggaacg cctcgccgcg
     2821 atggctgatc tcgacgatcg aggcacccga gtcgcggtac tccagcatct ccgcctgggc
     2881 ctggcgcaag accggctccg gcagtgcggc gggaccgggg ctgaaattgt aggcacgcga
     2941 catgtgcaag gtccgacggg tggaaacccc gagtatgccg cagtgcagca gcctgcgcgg
     3001 gcatggttgc gacggcaaca tattgcggac gattgggccg gtatgcgtga aacttcgcag
     3061 acggctttgg ctctaagctt gcgacaccct tccgcaacgc tggagcaagg ccgccatgaa
     3121 cctgcgcgac gcactccgca tcccgtccgc cgacgtgacc gaagaatccg tgtatcggga
     3181 ccgccgccgc gtgctggcga tgatcggagc ggtacctgca cttggcctcg ccggttgttc
     3241 cgaggccgcg ccgccggcgt ccaaggccac gatcacgccg gagctggcca aggcaggctt
     3301 ccgtacagcc gaggaacaga cccgggaaat cgacgccacc acctacaaca atttctatga
     3361 gttcggcacc gccaaggacg atccggcgaa cgctcccaag accttgcgca ccgcgccgtg
     3421 gacggtggcg gtgggcggtc agtgcgccaa gcccggcaag ttgtcgctgg aggacctgat
     3481 caaggggctg gcgccgcagg aacggatcta ccggatgcgt tgcgtggaag gctggtcgat
     3541 ggtgatcccg tggctcgggg tgccgctggg cgacgtgctc aagcgcttcg agccgacctc
     3601 gaaggcgaag tacgtggcct tcaccagcct ggccgatccg cagcagatgc ccggcgtgcg
     3661 cgcgcgggtg ctggactggc cctatcgcga gggcctgcgc atcgacgagg cgatgcatcc
     3721 gctgaccctg ctcgcgaccg gcatgtacgg caaggcgctg ccgcagcaga acggcgcgcc
     3781 gctgcgcctc gtggtcccct ggaaatatgg gttcaagggc atcaagtcga tcatcgcgat
     3841 caccttcgtg gagcgcatgc cgaagacctc gtggaacatg tcgcaggcct cggagtacgg
     3901 attcttctcc aacgtcaacc cggcagtcga ccatccgcgc tggagccagg ccagcgagcg
     3961 gcgcatcgcc ggcagcgcca gcaagctgtt cgccgagcgc gtccccaccc tgccgttcaa
     4021 tggctacgcc gcgcaggtgg cgtcgctgta cgcgggcatg gacctgaaga agtggttctg
     4081 accacgcgca cgcggcgtcg cgccgaaccg cgcatcgtcg tcgccaaggc ggccgtccac
     4141 ctcgccgcac tcaccccgct ggcgatcctg ctgtggcaga tgtggggcga gctgaagggc
     4201 gtcagccatg cactgggcgc cgatcccgtc gccgagatcg agcaccgcct gggcctgtgg
     4261 gcgctgcgct tcctgatgct caccctggcg atcaccccgc tgcgccagct gaccggctgg
     4321 aacgtgctgg tgcgtttccg gcggatgctg ggcctgtacg ccttcgccta cgccagcctg
     4381 cacttcgccg cctacctggg cctggacctg cgcggcttct gggcgcaggt gttccaggac
     4441 atcgtcaagc gtccctacat taccgtcggg ttcatcgcct ggctgctgct ggtgccgctg
     4501 gcggtcacgt ccaccaccgg ctggatcaag cgcctgggcc gcaactgggc gcgcctgcac
     4561 aagctggtct acgggatcgc cgtgctggcg gtgctgcact tctggtggct ggtcaagtcc
     4621 gacctgcgcg agccggcgct gtacgccggg gtcgccgcgc tgctgctggg ctggcgcgcg
     4681 tggaaggcgc tgaagacgcg cgccggcgct cgggcgaagg ctagaagcgt tccttcagca
     4741 cgcgcttgat ttcgttctcg atcggatcct tgaacatcgc ggtcaggaag ccgagcttgg
     4801 cgtgcacgtg caggtcgtcc ttgcccagtg cgatctggcc ctccacgccc gagcgggtga
     4861 agtggaggac atcgccgtcc caggcgcatt ccatctggaa gcgctccgcc agcttcttcg
     4921 cgacctcgtc gatcgccttc ttcgccttgg ccttgggcag gtcgtgcgga tggcggatat
     4981 cgatgcggga catgcggcga tccagtgagg tggcagtgcg cattgtgccg cgcgccgatc
     5041 accacaacca gcctgcaagg cgcagcgtgc atgctagatt gcggcccgat gagtgaattg
     5101 cgcctgcgtt tcagcgatgg tgaccagccc gacctcgtgg tcggcgccgg cgtgcatgcg
     5161 ctcggccgcc ttccgaccgg gctgggaccg gtcgatgcgg aacagccgtg gctgctgcgg
     5221 gtaagcaatg atcgtcgtgg cctgtggctg accgtggccg agggcctgcg cggcgtccac
     5281 gtcaacggcc ggccagtgca gcatgtggcg atgttgcgcg ccggcgacag catccacgtc
     5341 gatggcggcg agctcctgct ggtggcggcg aacgacggcc gcgcggtggc ccctgtgatc
     5401 gccgggcgcg acccggtcgg caacctgagg ctggtcctgc gcggcctggg cggcagccac
     5461 catgggcgca gcctgaacct cgagaagccg cgccggatcg gcagcgcggc cgacgccgac
     5521 ctgcgcatcg acggtcccgg catcgccccg caacacgcag tgcttgaagc cgtgaacggg
     5581 caagccgtcc tgcgcgatgc cgccgcggac gtgcaggtca acgggcaacg cgtgcgccat
     5641 gcgctgctgc aagccggcga ccaggtcgca ttcgacgtgc agcatcgctt cgtcctcgaa
     5701 ggcccgccgc ccgcagccgc ggtgccgccg ccgcgcgcgc gcgatggcaa cggagatgcg
     5761 gccgatgagg tgcctgcagg cggtcgccgg cactggacgc tgcggatccc gtggctgctg
     5821 cttgcggcga tcctgctgtc ggccgcattg tcggtgctgc tcctgttcgg cgagcgctga
     5881 ggcgcgatca gggctggcgg aacggatccg gatcggcggc gaacaagcgc tgcatgaccc
     5941 gcgtctcgtt ggcgcggatg tccgccagga acggcccggc gtcgccgaga ccgccgaacg
     6001 cgtcgaagcc ccgctccagg aagccctgca actcgccgaa gcccatcgcc cgcgccggca
     6061 tccgcgagat cttcaacagc gcgcccacgc cgtgcatccg caaggcggac cacaggccgt
     6121 ggccgacttc gtcgatcagg ctgatctggc gtgcgcgcag gcgtcgatga ccgaccgcgc
     6181 gataggcgcg tgcatacagg tcggcgtcga tgctgcggcg gcgaccgccc agcgcctgca
     6241 gggcttcgac catgcgcagg tcgagcgcat gggtcagcac ggccagttcg atgccatccg
     6301 cgacggtctt gagcagggcc gcgggcagca gcttctgcat catcgggatc actcgcgcga
     6361 tgtcggcatc acgctggctg aagtcatgct cgccgtacag gtcggtgagg aagaactgcg
     6421 cggcgggacg tcgcaccgga tcgccgagga agcccgcgaa gctgcgctgc aggcgctgcg
     6481 cctgccagga ctgcaagggt ttcagccatc gcgacgcatt gcgcggttcg cgttcggggt
     6541 cgtacatccc ctggtgccac gccaggcgac gggtgaggcg cgcgatcggg tcggtacgca
     6601 ccgggttcct tgcgggcttc cttgcaggca tcgccacagt gtcgaggcgg cggcgcatcg
     6661 acgcaatact ccaacccact ccgctacact cggccgcgac ccacgcgagc gaaccgaatg
     6721 ctgtcactgc acgctgccca gcaacgccac cccggccgca gccatgcccc gcgcatcggc
     6781 ctggccatcg ccggtggcgg gccgatcggg gcgatgtacg aacttggcgc actgcgcgcc
     6841 ctggacgagg cctgcgaggg cctggatctg acccgactgg actgctacgt gggcgtgagc
     6901 tcgggcgcgt tcttcgccgc cggcctggcc aaccgcatgg acaccgccga gctctgccgg
     6961 atcttcatca ccggcgaaag cgacgacgtg gtgttccgcc cggaaacctt cctgcgcctg
     7021 gccaccgacg aatacctgcg acgcgccgtc gcgttcccga aactggccgc acggctcacc
     7081 cgcgacatgc tgtccgggcg cggcgactcg cgctggtccg acatcgtcac ccgcatcggc
     7141 gggctggtcc cgaccggcat cttcgacaac gccggcgtcg agcatttcct gcgcgaggtc
     7201 ttcacccggc gtggccgcag caacgatttc cgcaagctgg agcgcccgct gtacgtgatc
     7261 ggcgtggacc tggacagcgg cgaggcggtg cgcttcggcg acgccggctg ggacagcgtg
     7321 ccgatctcgc gcgcggtgca ggccagcgcc gccctgcccg gcctgtatcc gccggtcgag
     7381 atccgcggcc gccacttcgt cgatggcgcg ctgcgccgga cgatgcacgc ctccgtcgtg
     7441 ctggaacgcg acgtcgacct gatgctcggc atcaacccgt tggtgccgtt caatcccggc
     7501 acggatgagt atgccgatgc gcaggcccac cgcttcagcg accgccggat cgccgagggc
     7561 ggcttcccgg gagtgatctc gcaggcgttg cggacgatgc tgcagtcgcg catgcaggtc
     7621 ggcctggcgc gctacccggc ccagtacccg cacatcgacc aggtcgtgtt cgagcccaac
     7681 gccgacaacc gcgaattgtt ctacaccaac atcttcagct tcgccgcgcg ccgccgcatc
     7741 tcccagcttg cctatcgcaa caccctggcc gacctgcgca agcgtcgcga tgaactggag
     7801 ccggtgctcg cgcgccatgg catccgcctg cgcgccgacg tgatcgccga ccgaccacgc
     7861 tcgatcatcg cccacctgcc ggcgcagccg cgcagcacgg aaaccaccgc cgtcctcgat
     7921 cgcgcgctcg acgagatcga gcatcgcctg cgcaccgaca agcgcgcgca ggcccgcggc
     7981 atggtgtgaa tgctctagac gccgacgcga tcctgcgtcg caatccaatc cccgcgcgtc
     8041 gccgacgcac acagagaaac gacatggcca agctcaagaa gactgcgcgc aagaccgcct
     8101 cgggcaccaa gcaggcgtcc caggaccagg ccgagcgcct gggcaagtcg atcaccgaat
     8161 ccgcgcagca gatctggctg gccggcatgg gcgcgttcaa ccgcgcgcag gccgagggcg
     8221 gcaagctgtt cgagagcctg gtccgtgacg ggctgtcgct ggagcagacc gcccgcaagc
     8281 tcgccggcaa ccgcgccaac gtggtgcgcg acgtggtgga gggccgcgtc ggccaggcgc
     8341 gcgaacgcgc cgtcgacacc tgggacaagc tggagaaggt gttcgaggat cgcgtgcagc
     8401 gcgcgctggt caagctcggc gtgccgggcc gcgacgacct gaacgccctg acccagcgcg
     8461 tcgagcgcct caccgaggag ctgcgcaagg ccaatggcgg cagcgcgcct aaggcggcca
     8521 gggcaccgcg caaggcggct gcgccgaagc cggcgaaggc gcctcgccag gcgaaggccc
     8581 ccaaggcaac gaaggccgcc aaggccccgg cccgcaaggc tgccaaggcg gccgcggaag
     8641 ccccggccag ctgatccgtc caagccccgc cagtccgtcg cttcgcacgc tccctccccc
     8701 tgcgatcccc ggactggcgg gcattccccc cgccggggat cgcggtgatg gcggcgcatc
     8761 gacccggcaa gggctatagt ccctgccccg tgttgttgct tttgccttta tgtccccgat
     8821 cctgatcgcg ttgctggtat tcgttgtcct tggactggcg gccacggcct ggttgcggcg
     8881 cccgccgtcg cctccctcca cgtcgcaggc aaacgcgcga ggtgccgccc agctgggtac
     8941 cttgcgctgg cgcgacttca cccgcctggt gctgcaggcc atgcatggcc gcggttaccg
     9001 caccgtggtc gaagacggaa tgccagccga cggcatcccc accgacggtg gcgacatcgt
     9061 cctggagcgt ggcggcgag
//
LOCUS       DAOOCX010000091         8621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_7717_length_8621_cov_8.100280, whole genome shotgun sequence.
ACCESSION   DAOOCX010000091 DAOOCX010000000
VERSION     DAOOCX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7717_length_8621_cov_8.100280"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1354)
                     /gene="tyrS"
                     /locus_tag="PK399_08955"
     CDS             complement(143..1354)
                     /gene="tyrS"
                     /locus_tag="PK399_08955"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPW13285.1"
                     /translation="MSDHQHALDLIARGSDEILKREELAARLEEGRPLRVKAGFDPTA
                     PDLHIGHTVLLNKMRQFQDLGHQVIFLIGDFTGMIGDPTGKNATRKPLTRDDVLANAQ
                     TYADQVFKVLDRERTEVRFNSEWFGAMTAADMIRLAAQHTVARMLERDDFAKRYAAQQ
                     PIAIHEFLYPLVQGYDSVALRADVELGGTDQKFNLLMGRGLQEAHGQAPQVVLTMPLL
                     EGLDGVNKMSKSLGNYIGIAEPAIDIVTKAMKIGDDLMWKWIDLLSFDIGMDEAAALR
                     ADIASGQLNPRDVKLRLARELATRFHDAATAEQAVAGWHAAVRGEGDLSSLPLQELAV
                     PADGLRIAALLVAAGLMPSNSEANRKLKERAVRVDGEVFEDAARTFVPGFEGVLAVGK
                     RNFARLRLVAA"
     gene            1522..2952
                     /locus_tag="PK399_08960"
     CDS             1522..2952
                     /locus_tag="PK399_08960"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW13286.1"
                     /translation="MTDSHHDAVRRENLERARANGAQGLSPLAITHPFNGRWTRRHWA
                     HASLFATIGALLAAIVPGFSTALEAAPNTQRTTLSLALPQLRLTRADNDHWQSVTLKP
                     GQTLSGVFDELGIPYSQLEKVMQHPKIKPALRKLRPGTALSFNLPSDGSVRAMRIEAA
                     PGIGDEPVELEFDGDALRERAVPQEITTRTVVLSGEVGKSLFASARKLGLGSAQLNQL
                     TDEMFKYDIDFDSDLDEDDRFSVVVDQTWKNGQLASTGPVLAATFTMDGQLKSAFRHM
                     RGGKPEYFTPDGRSLRRPFIRMPIPYARLSSGFGGRKHPVLGRFRMHKGVDYAAGTGT
                     PIQAAGDARVELVGWKGGYGRTVILNHGGGRTTLYGHMSRFAGIKPGQRISQGTVIGY
                     VGSTGLATGPHLHYEFRVNGVHMNPLKMTLPPPEPLSGGALVAFKSETRRALDKIREV
                     EDVVFQLDDGSRVAATDKPAGTRKKG"
     gene            2984..4111
                     /locus_tag="PK399_08965"
     CDS             2984..4111
                     /locus_tag="PK399_08965"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011257438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPW13287.1"
                     /translation="MPNLQPARLFLGLISGTSADGIDAALVRFADDTPHARPELVFGR
                     TYAWDAVLRARLVALGQQSAQLTLDEVGELDVRIGRAFADAANAAIADCGIDRGALAA
                     IGSHGQTLRHRAHGEVPYTLQLGDPSTIAERCGVAVVADFRRRDVAAGGHGAPLVPAF
                     HAATLHHAGEDRAVLNLGGIANLTLLPVHGAVRGFDTGPANGLMDAWCLRHTGHGFDA
                     GGAFAAAGQVDDGLLARLLDEPWFALPPPKSTGRDQFHLDWVASKLAGGESAADVQAT
                     LLALTVRTIAEALQASHSGTRRLIACGGGVHNPVLMAALAAAMPGLHVESSAVHGLDP
                     DSVEAMAFAWLARETLANRPGNLPAVTGAAGPRVLGGIYPP"
     gene            complement(4113..4370)
                     /locus_tag="PK399_08970"
     CDS             complement(4113..4370)
                     /locus_tag="PK399_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13288.1"
                     /translation="MTDLTVHNIRPKLDERLRRLAELQGLTVDTVAAEALAIGVDAIE
                     DRVRKHLLNTREQVALSEAITQIEKVPDSAFGMIGRVARRD"
     gene            complement(4413..5777)
                     /locus_tag="PK399_08975"
     CDS             complement(4413..5777)
                     /locus_tag="PK399_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW13289.1"
                     /translation="MTVATTPRKNTAGDVLRALRQPKVLAMLLLGFSSGLPLYLVGNT
                     MGYWMREGGSELSAIGFLSWVGLAYTLKFLWAPIIDKTDVPLLGRLGHRRGWMLLSQL
                     FVAAGLAGMAIVQPEGGLVVFGALALMAAFASATQDIVIDAWRIEIADNTEELGLLTS
                     GTQIGYRSALLVTDALILILATYIGWAPSYWVMALLMGVGVLAALLLAREPAASRSPQ
                     VAGHVPLWTISGLFDAIVGPFLAFFRTHGKWALLMLAAISLYRLPDFVMGPMNNPFYA
                     DIGISKEFVGEVRASLGLVASVLGIAAAGISAVRFGFVPTLLAGAILGPGSNAAFTWV
                     AYAGGDHTVFAAAMFIDGFSTGFAGTALVAYMSSLTSIGYTATQYALLSSFYALLGKV
                     LKGFSGVAVEALQVGHGQIGGYALFFLGTALTGIPALLLCLLLARRIRADAAPGLPGR
                     APPG"
     gene            complement(5774..6577)
                     /locus_tag="PK399_08980"
     CDS             complement(5774..6577)
                     /locus_tag="PK399_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPW13290.1"
                     /translation="MRILSFNANGLRSAASKGFFDWFGKQDVDVLCLQETKAQEHQLS
                     DAVFRPDGYRAWFKDASTKKGYSGVAIYSRREPDDVRTSLGWAPFDDEGRYIEARFGN
                     LSVVSFYIPSGSSGELRQGFKEEVMAWLKPIMDQWLASGREYVLCGDWNIVRAKNDIR
                     NWTGNQKNSGCLPHERAWLNGLIADDCHDGLIVPPARGWKDAFRVAKPAAVEYSWWSQ
                     RGAARANNVGWRIDYQIATPGIASTVTAAEIFPEPRFSDHAPCLVEYAG"
     gene            6673..7335
                     /gene="pyrE"
                     /locus_tag="PK399_08985"
     CDS             6673..7335
                     /gene="pyrE"
                     /locus_tag="PK399_08985"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPW13291.1"
                     /translation="MDPADNDHRTRFLKLALRAEALRFGEFTLKSGRTSPYFFNAGRF
                     DSGAALAELAGCYADAVDHAGLGFDLLFGPAYKGIPLATALACEYARRGRDLPLAFNR
                     KEAKAHGEGGSLIGAPLAGRRVLIVDDVITAGTAIREALDIIAAAGGIAAGIVIALDR
                     QERVREDAGQSAAQAVAAEHGIPVVAVAGLSDLLEFTGESADLVRQRDALLAYRARYG
                     IA"
     gene            7371..8093
                     /locus_tag="PK399_08990"
     CDS             7371..8093
                     /locus_tag="PK399_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13292.1"
                     /translation="MTHLRTATLAATALLLLGTGIAGAQTKPKATRPGEPPAVEKKIY
                     CWSEGGRKVCGDALPASAANSARTEISSRSGMTTSRVDRALTGSERSAAEQAAEVARQ
                     QAEALALQQRRDLAMVESYMTEADLRRAYGERTALLDETLKASKLGLSNLRLSLLALL
                     RQAGDQELAGPVAKRLGKSIREQHGELLRQQQILVTQQQDRAALEHDLQDSLRRYREL
                     KRDDAPAATTAAPVPKPAAAAG"
     gene            complement(8109..>8621)
                     /locus_tag="PK399_08995"
     CDS             complement(8109..>8621)
                     /locus_tag="PK399_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPW13293.1"
                     /translation="ILRHGGSPLMWKTGHSLIKAKMRETEAELAGEMSGHFFFAERWF
                     GFDDGIYAAARLLEILDAGGMSPEELFATLPDGISTPELKVEAPDGEPHAFVDRFKLV
                     AKFEGARLSTLDGLRVDWPDGWGLVRASNTTPILVMRFDADTNAAMARIQQAFREQLL
                     LVQPDLKLPF"
BASE COUNT         1412 a         2992 c         2898 g         1319 t
ORIGIN      
        1 catcatacaa gggtttttgt tgccgtcaac accctgtgat gatttttttc gcccggtgcc
       61 gacatgaact cggaagcggg tcgatgattc tagcggacct tgctgcagga cagcaagctc
      121 gatttgcaac tttctttcgc catcacgcag caacgaggcg cagcctcgcg aaattgcgct
      181 tgccgacggc cagcacgccc tcaaaaccgg gcacgaacgt acgagcagca tcttcgaaga
      241 cctcaccatc gacgcgcacc gcacgctcct tcagcttgcg gttcgcctcc gagttgctgg
      301 gcatcaggcc ggccgccacc agcaaggcgg cgatgcgcag gccatccgct ggcaccgcca
      361 actcctgcag cggcaggctg gacaggtcgc cctcgccgcg cactgccgca tgccagccgg
      421 cgaccgcctg ctcggcggtg gcggcatcgt ggaagcgcgt ggcaagttcg cgggccagcc
      481 ggagcttgac gtcgcgcggg ttgagctggc cgctggcgat gtcggcccgc agcgccgcgg
      541 cctcgtccat gccgatgtcg aagctgagca ggtcgatcca cttccacatc aagtcgtcgc
      601 cgatcttcat cgccttggtg acgatgtcga tggccggctc ggcgatgccg atgtaattgc
      661 ccagcgactt gctcatcttg ttgacgccgt ccagcccctc cagcaacggc atggtcagca
      721 ccacctgcgg cgcctgtcca tgcgcttcct gcaggccgcg ccccatcagc aggttgaatt
      781 tctggtcggt gccgccgagc tcgacgtcgg ccctcaacgc caccgagtcg tagccctgga
      841 ccagcggata caggaattcg tggatggcga tcggctgctg cgcggcgtaa cgcttggcga
      901 agtcgtcgcg ctccagcatg cgcgcgaccg tgtgctgcgc ggccagccgg atcatgtcgg
      961 cggcggtcat cgcgccgaac cattcgctgt tgaagcgcac ttcggtgcgt tcgcgatcca
     1021 gcaccttgaa cacctggtcg gcgtacgtct gcgcgttcgc gagcacgtcg tcgcgggtga
     1081 gcggcttgcg ggtggcgttc ttgccggtgg ggtcgccgat catcccggtg aagtcgccga
     1141 tcaggaagat gacctggtgg ccgaggtcct ggaactgccg catcttgttc agcagcacgg
     1201 tatggccgat gtgcaggtcg ggcgcggtcg ggtcgaagcc ggccttgacc cgcaacgggc
     1261 ggccctcctc caggcgggcg gccaattcct cgcgcttgag gatttcgtcg ctgccgcggg
     1321 cgatcaggtc gagggcgtgc tggtggtcgg acacagaaag gctcgctggc ggtcgattcg
     1381 tgaccgggtt cggggaatga ggttaaggta acgttaaatc gaatctgcga aaaacattcg
     1441 gagattcaac gagttgacgc atgttgttcg catgattatg gtaccgcgtc gacgcattcg
     1501 tcacacgcgg ggaaaagtca catgacggat tcgcaccacg acgccgtgcg tcgcgagaat
     1561 ctcgagcgtg cacgcgccaa tggcgcgcaa ggcctctcgc cgctcgccat cacacatccc
     1621 ttcaatggtc gctggacacg ccgccactgg gcgcacgcca gcctgttcgc gaccatcggc
     1681 gccttgctgg cagcgatcgt ccccggcttc tccaccgcgc tggaagccgc gccgaatacc
     1741 caacgcacca ccctgtcgct ggccttgccg cagttgcgcc tgacccgcgc cgacaacgat
     1801 cactggcaat cggtgaccct caagccgggg cagaccctga gcggcgtatt cgatgagctc
     1861 ggcattccct acagccagct cgagaaggtc atgcaacacc cgaagatcaa gccggcgctg
     1921 cgcaagctgc ggcccggcac cgcgctcagc ttcaacctgc cgagcgatgg cagcgtgcgg
     1981 gcgatgcgca tcgaggcggc gccgggcatc ggcgacgagc cggtggaact ggaattcgac
     2041 ggcgacgccc tgcgcgagcg cgcggtgccg caggaaatca ccacccgcac ggtggtgttg
     2101 agcggtgaag tcggcaagtc gctgttcgcc tccgcgcgca agctcgggct tggcagcgcg
     2161 cagctcaacc agctgaccga cgagatgttc aagtacgaca tcgacttcga ttccgacctg
     2221 gacgaggacg atcgtttcag cgtggtggtc gaccagacct ggaagaacgg ccagctggcc
     2281 agcaccggcc cggtgctggc ggccaccttc accatggatg gccagctgaa gagcgcgttc
     2341 cgccacatgc gtggcggcaa gccggaatat ttcaccccgg acggtcgctc cctgcggcgc
     2401 cccttcatcc gcatgccgat cccctacgcg cgcctgagtt ccggcttcgg cgggcgcaag
     2461 cacccggtgc tgggccgttt ccgcatgcac aagggcgtgg actacgcggc cggcaccggc
     2521 accccgatcc aggccgccgg cgatgcgcgg gtggagctgg tcggctggaa gggcggctac
     2581 ggccgcacgg tgatcctcaa ccatggcggt gggcgcacga cgctgtacgg gcacatgtcg
     2641 cgcttcgccg ggatcaagcc gggccagcgc atctcgcagg gcactgtgat tggttatgtc
     2701 ggcagcaccg gcctggccac cggaccgcac ctgcactacg aattccgcgt caacggcgtg
     2761 cacatgaacc cgctgaagat gaccctgccg ccgccggagc cgctgagcgg tggcgccctg
     2821 gtcgccttca agagcgagac caggcgcgcc ctggacaaga tccgcgaggt cgaggacgtg
     2881 gtcttccagc tcgacgacgg cagccgcgtg gccgcgaccg acaaacccgc cggtacccgc
     2941 aagaagggtt gatcgcccac gcggtgcagc gatactgcgt cgcatgccca acctgcaacc
     3001 tgcgcgactc ttcctcggct tgatttccgg caccagcgcc gacggcatcg atgccgcgct
     3061 ggtgcgcttc gccgacgaca ccccgcatgc acggcccgag ctggtgttcg gccgcaccta
     3121 tgcgtgggat gccgtattgc gcgcgcgcct ggtcgcactg ggccagcaat ccgcgcagct
     3181 cacgctggac gaggtcggcg aactcgacgt gcgcatcggc cgtgcgttcg cggatgcagc
     3241 gaatgcggcg atcgcagact gcggcatcga ccgtggcgcc ctggccgcga tcggctcgca
     3301 tgggcagacc ctgcgccatc gcgcccatgg cgaggtgccc tacaccctgc agctgggcga
     3361 tccgtccacc atcgccgagc gctgcggcgt ggcggtggtg gccgatttcc gtcggcgcga
     3421 cgtcgctgcc ggcggccatg gcgcaccgct ggtcccggcc ttccacgccg ccaccctgca
     3481 ccacgccggc gaggatcgcg ccgtgctcaa cctgggcggc atcgccaacc tcaccttgct
     3541 gccggtgcat ggcgccgtgc gcgggttcga caccggcccg gcgaacggcc tgatggacgc
     3601 gtggtgcctg cgccacaccg gccacgggtt cgatgcgggc ggcgcgttcg cggcagccgg
     3661 acaggtcgac gatggcctgc tcgcccggct gctggacgag ccgtggttcg cgttgccgcc
     3721 accgaaatcg accggccgcg accagttcca cctggactgg gtcgcgtcga agctggccgg
     3781 aggtgagtcc gccgccgatg tgcaggcgac cctgcttgcg ctcaccgtcc gcacgatcgc
     3841 cgaggcgctg caggcgagcc actccggcac ccgccggctg attgcctgcg gtggcggggt
     3901 gcacaacccg gtgctgatgg cggcgctggc cgccgcgatg cccggcctcc atgtcgaatc
     3961 cagcgcggtg cacgggctgg atccggacag cgtggaggcg atggccttcg cgtggctggc
     4021 ccgggaaacc ctggccaacc ggccgggcaa cctgccggcg gtgaccggtg ccgccgggcc
     4081 acgcgtgctc ggcgggatct acccgccctg actcagtcgc gccgggcgac gcggccgatc
     4141 atcccgaagg cggagtccgg cactttctcg atctgggtga tcgcttccga cagcgcgact
     4201 tgctcgcggg tattgagcaa gtgcttgcgc acgcggtcct cgatggcatc cacgccgatc
     4261 gccagggctt cggcggcgac cgtatcgacg gtcaatccct gcaactcggc gagccggcgc
     4321 aggcgttcgt cgagcttggg gcggatgttg tgcacggtga gatcggtcat gacagggaaa
     4381 tccttgcttg cgcgggggaa cgcgcccctg ttctaacccg gcggggcccg cccgggcaag
     4441 cccggcgcgg catcggcgcg gatccgccgt gccagcagca ggcacagcag caatgccggg
     4501 ataccggtca gcgccgtgcc caggaagaac agcgcgtagc cgccgatctg gccgtggccg
     4561 acctgcaggg cttccacggc gacgcccgaa aagcccttca gcaccttgcc gagcagcgcg
     4621 tagaaggagc tgagcagcgc gtactgggtg gcggtgtagc cgatgctggt caggctggac
     4681 atgtaggcga ccagcgcggt gccggcgaac ccggtggaga acccgtcgat gaacatcgcc
     4741 gcggcgaaga cagtgtggtc gccccccgca taggccaccc acgtgaaggc cgcattggag
     4801 cccggcccga ggatcgcgcc ggccagcagg gtcggcacga agccgaaacg caccgcgctg
     4861 atcccggccg cggcgatgcc cagtaccgag gcgaccagtc cgagcgacgc ccgcacctcg
     4921 ccgacgaatt ccttgctgat cccgatgtcg gcgtagaacg ggttgttcat cggccccatc
     4981 acgaagtccg gcagccggta caggctgatc gccgccagca tcagcagcgc ccacttgccg
     5041 tgggtacgga agaacgccag gaagggaccg acgatggcat cgaacagccc ggagatcgtc
     5101 cacagcggca cgtgcccggc gacctggggc gatcgcgatg ccgcgggttc gcgcgcgagc
     5161 aacagcgcgg caaggacacc gacgcccatc agcagcgcca tcacccagta ggacggcgcc
     5221 cagccgatgt aggtggccag gatcaggatc aacgcatcgg tcaccagcag cgcgctgcga
     5281 tagccgatct gcgtgccgga cgtcagcagg ccgagttcct cggtgttgtc ggcgatctcg
     5341 atgcgccagg catcgatcac gatgtcctgg gtcgccgagg cgaacgcggc catcagcgcc
     5401 agcgcgccga acaccaccag gccgccttcc ggctgcacga tggccatgcc cgccagcccg
     5461 gccgcgacga acagctgcga cagcagcatc cagccgcggc gatggcccaa gcggcccagc
     5521 agcggcacgt cggtcttgtc gatgatcggt gcccacagga acttgagcgt gtacgccaac
     5581 ccgacccagg acaggaagcc gatggccgac agttcgctgc cgccctcgcg catccagtag
     5641 cccatggtgt tgccgaccag gtacagcggc agtccggacg agaacccgag cagcagcatc
     5701 gccagcacct tgggctggcg cagcgcgcgc aggacatcgc cggcagtgtt cttgcgtggt
     5761 gtcgtcgcca cggtcacccg gcgtactcca ccaggcacgg ggcgtggtcg gagaaacgcg
     5821 gctccgggaa gatctcggcg gcggtgaccg tggacgcgat cccgggcgtg gcgatctggt
     5881 agtcgatccg ccagcccacg ttgttggcgc gcgccgcacc gcgctgcgac caccaggagt
     5941 attcgacggc agcgggcttg gcgacccgga acgcgtcctt ccagccgcgt gccggcggta
     6001 cgatcaggcc gtcgtggcag tcgtcggcga tcagtccgtt cagccacgcg cgttcatgcg
     6061 ggaggcaccc agaatttttc tggttgccgg tccagttcct gatgtcgttc ttcgcccgca
     6121 cgatgttcca gtcgccgcac agcacgtact cgcggccgct cgccagccac tggtccatga
     6181 tcggcttcag ccaggccatc acctcttcct tgaagccctg gcgcagctcg ccggagctgc
     6241 cggacgggat gtagaagctg accacgctga ggttgccgaa gcgggcctcg atgtagcggc
     6301 cttcgtcgtc gaacggcgcc cagcccagtg aggtccgcac gtcgtcgggt tcgcgccggc
     6361 tgtagatggc gacaccgctg tagcccttct tggtcgatgc gtccttgaac caggcccggt
     6421 agccgtccgg gcggaacacg gcgtcggaca actggtgttc ctgcgccttg gtttcctgca
     6481 ggcacagcac gtccacatcc tgcttgccga accagtcgaa gaagcccttg ctggccgccg
     6541 agcgcaggcc gttggcattg aaactgagga tgcgcatgcg gcggtccggc gggaacgatg
     6601 gccgagggta gccgatgcgc gtcgtcgcgc aagtcccgcg acgacgcgct gcacgcatgg
     6661 gagaatcggc tcatggatcc cgcagacaac gaccaccgca cccgcttcct gaaactcgcg
     6721 ttgcgcgccg aggcgctgcg cttcggcgag ttcaccctga agtccggccg caccagtccc
     6781 tacttcttca atgccgggcg cttcgactcc ggtgccgcgc tggcggaact ggccggctgc
     6841 tatgccgatg ccgtggacca tgccgggctg gggttcgacc tcctgttcgg ccccgcctac
     6901 aagggcatcc cgctggcgac cgcgctggcc tgcgaatatg cacgccgcgg ccgcgacctg
     6961 ccgctggcgt tcaaccgcaa ggaagccaag gcgcatggcg agggcggcag cctgatcggt
     7021 gcgccgctgg ccggcaggcg ggtgttgatc gtggacgacg tgatcaccgc cggcactgcg
     7081 atccgcgagg cgctggacat catcgcggcg gcgggcggca tcgcggccgg catcgtcatc
     7141 gccctggatc gccaggaacg ggtgcgtgaa gacgccgggc aatccgcggc gcaggcggtg
     7201 gcggcggaac acggcatccc cgtggtcgcc gtggccggcc tgtccgacct gcttgaattc
     7261 accggcgaaa gcgccgacct tgtacgccaa cgcgacgccc tgctcgccta ccgcgcgcgc
     7321 tacggcatcg catgacccgc cacgcttcac gactgccaca gggggctgtc atgacgcatc
     7381 tccgcactgc cacgctcgcc gcgaccgcct tgctcctgct gggcacgggc atcgcgggcg
     7441 cgcagaccaa gcccaaggcg acgaggccgg gggaaccgcc ggccgtcgag aagaagatct
     7501 attgctggag cgaaggcggc cgcaaggtct gcggcgatgc gttgccggcg tccgcggcca
     7561 acagcgcgcg caccgagatc agcagtcgca gcggcatgac caccagtcgc gtcgaccgcg
     7621 ccctgaccgg cagcgaacgc agcgccgcgg agcaggccgc cgaggtcgcc cgccaacagg
     7681 ccgaggcgct ggcgctgcag cagcgacgcg acctggcgat ggtcgagtcg tacatgaccg
     7741 aagccgacct gcgccgcgcc tacggcgagc gcaccgccct gctggatgaa accctcaaag
     7801 cgagcaagct tggcctgtcc aacctgcgcc tgagcctgct ggccctcctg cgccaggccg
     7861 gtgaccagga actggccggg ccggtcgcca agcggctggg caagtcgatc cgcgagcaac
     7921 atggcgagct gctgcgccag cagcagatcc tggtcaccca gcagcaggat cgcgctgcac
     7981 tggaacacga cttgcaggat tcgctgcgcc gctaccgcga attgaaacgc gatgacgcgc
     8041 ctgcggccac caccgcggcg cccgtaccga aacccgcggc ggcggcgggc tgagcccgcc
     8101 gcgaccggtc agaacggcag cttcaggtcc ggctgcacca gcagcaactg ctcgcggaag
     8161 gcctgctgga tgcgcgccat cgccgcgttg gtgtcggcgt cgaagcgcat cacaaggatc
     8221 ggcgtggtgt tggacgcacg caccaggccc cagccgtccg gccagtccac gcgcagcccg
     8281 tccagggtcg acaggcgcgc accctcgaac ttcgccacca gcttgaaccg gtcgacgaac
     8341 gcatgcggct cgccatcggg ggcttcgacc ttcagctcgg gggtggaaat gccgtcgggc
     8401 agggtggcga agagctcctc cggcgacatt ccgcccgcgt ccaggatctc cagcaggcgc
     8461 gccgccgcat agatgccgtc gtcgaagccg aaccagcgct cggcgaagaa gaagtggccg
     8521 ctcatctcgc cggccagttc cgcctcggtt tcgcgcatct tcgccttgat cagcgaatgc
     8581 ccggtcttcc acatcagcgg gctgccgccg tggcggagga t
//
LOCUS       DAOOCX010000092         8568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_7774_length_8568_cov_6.533889, whole genome shotgun sequence.
ACCESSION   DAOOCX010000092 DAOOCX010000000
VERSION     DAOOCX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7774_length_8568_cov_6.533889"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PK399_09000"
     CDS             complement(<1..1087)
                     /locus_tag="PK399_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HPW13294.1"
                     /translation="MPQVPDALARKIAQTIATEIGAQDAQVHAAVALLDEGATVPFIA
                     RYRKEVTGGLDDTQLRNLETRLSYLREMEDRRASILASITEQGKLTDALRAEIDAADS
                     KSRLEDLYLPYKQKRRTRAQIAREAGLQPLADGLLADPSRLPDTFAASFIDADKGVAD
                     AKAALEGARAILIERWSEDATLLGELRGWLEGNGLIRAKVVEGKEAAGEKFRDYFDHV
                     ESLAKIPSHRLLALFRGRREEFLQLDLDPGSDAEAGHLQAEHRIALHAGIAERGRPAD
                     AWLRNACRLAWRARIHMHLLLDLFNQAREKAEAEAINVFGDNLKDLLLAAPAGPKAVL
                     GLDPGLRTGVKVAVVDATGKLVATDTIY"
     gene            complement(1244..2524)
                     /locus_tag="PK399_09005"
     CDS             complement(1244..2524)
                     /locus_tag="PK399_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW13295.1"
                     /translation="MTTADAAPGLTRQQARTLSLAALGGALEFYDFVIFVFFATTMGA
                     LFFPADMPEWLKLVQTFGIFAAGYLARPLGGIVMAHFGDLSGRKRMFMLSILMMAIPT
                     LLMGLLPTYATVGVLAPILLLVLRIMQGAAIGGEAPGAWVFVTEHVSTRHRALACGAL
                     SAGLCSGILLGSLVARAINAAFDEAEVLAWAWRLPFIAGGVFGLLAMFLRRKLHETPV
                     FAEMRERRSLAAELPLKAVVRDHAGAVVLAMLLTWLLTAAVVVTLLMMPTLLQGLGVS
                     RADALAGNTLAICATVIANLVAGWMADRFGAGRVLVLWSVLLGIAFWTFYAGALAGGY
                     SPALYVIAGFGVGVTALVPAIAVSGFPAQVRFSGLSFSYNVAYAIAGGLTPIALSLAM
                     KDSPAAPMHYIAGMALLGVALGLHVMMRRPLVGE"
     gene            complement(2578..>3417)
                     /locus_tag="PK399_09010"
     CDS             complement(2578..>3417)
                     /locus_tag="PK399_09010"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13296.1"
                     /translation="LVATANPLALTATLRMHGRALVPDRDALAATHPKTGPRLLVLVH
                     GLCMNDLQWQQGPHHHGNQLAEALGFEVIHLHYNSGLSIADNGREFSRLLQALVDAWP
                     VPVQRIAILAHSMGGLVSRAAIASAQADGSPWLHRLDTLVSLGTPHLGAPLERAGALL
                     QTVLGVSAHSAPFTALGGLRSAGIQDLRHGSLHVRHPPLPAQVRAFAVAASTRGPRSH
                     ASARRLHGDGLVPVASAFGEHTDPARDLAIPEHHRLLVHRTGHLGLLASAQVSRQLHD
                     WLA"
     assembly_gap    3418..3517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3518..3926)
                     /locus_tag="PK399_09015"
     CDS             complement(<3518..3926)
                     /locus_tag="PK399_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13297.1"
                     /translation="MSSPVRPDPLPRPLVLDGSDLRGANGLLVDAVTGTTDLVEAVHA
                     SILGWPRRLLGLSPPPTTGGIPALAYGAVRGIARVAGNGLDRVLRGLPTRSGQADRIP
                     QREALIAALNGVLGDHLVATANPLALTATLRMHG"
     gene            4114..6030
                     /locus_tag="PK399_09020"
     CDS             4114..6030
                     /locus_tag="PK399_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017118666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPW13298.1"
                     /translation="MTGLALALATAGGAMAQTAPMTPDITGRKFSAPETQRDFIKRVE
                     MVPMRDGTKLYTVIVIPKGAKDAPILFTRTPYNAAKRADRVPNATRMLDAMPQGDSVF
                     VEDGYIRVFQDVRGKYGSEGEYVMTRPPRGPLNGSGIDNATDAWDSIDWLVKHVPESN
                     GRVGMLGSSYEGFTVAMALLEPHPALKVAAPESPMIDGWMGDDWFQHGAYRQNSLGYY
                     TGQMTARGEGTSIPRATSDDYTSFLRGGALGDLARAAGLEQIPAWRKVIEHPAYDRYW
                     QQQALDKLLAKLPAISVPTMWEQGLWDQEDIYGAHTAWAALEPKDTDNTRNHLVIGPW
                     RHSGANYDGSSLGDLRFEGDTALQWRREVLKPFFDQYLKPGAAVAKTPPVWIYDAGSQ
                     RWDTYAQWPQSCAQGCPNAAVPLYLHADETLDGTRPVAAGAGYAEYVSDPAKPVPFVP
                     RPVNMDDRPTWTTWLVRDQRFVDGRTDVLSYTSAPLAKPLALAGIPVANLFASTSGSD
                     SDWVVKLIDVYPDQYPDDPKMAGYQLPLAITIFRGRYREDFAAPKPLQPGVAQAYTFD
                     LPTVHHVLKPGHRLMVQVQSSLFPLYDRNPQTYVDNIFFAKPADYRKAVQRIVATPEQ
                     ASFISLPVVSGTLP"
     gene            complement(6031..7122)
                     /gene="dinB"
                     /locus_tag="PK399_09025"
     CDS             complement(6031..7122)
                     /gene="dinB"
                     /locus_tag="PK399_09025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPW13299.1"
                     /translation="MSDTPQRKIIHVDMDAFYASVEQRDDPSLRGKPVVVAWRGARSV
                     VCAASYEARVFGVRSAMPAVRAERLCPDAVFVPPDFIRYKAVSKQVRGIFERHTDLIE
                     PLSLDEAYLDVTAPKQDLGSATAIALSIRDAIREETQLTASAGIAPNKFLAKIASDWN
                     KPDGQFVLKPAMVDAFLAPLAVGKLPGVGKVMEAKLAGLGIATCTDLRLHGAAALEQR
                     FGRWGRRLHELALGIDERSVQPERLTLQVSAEDTFPEDLPLDALEDHIRRLATKAWAG
                     YLRERERHPERIARTVVLKLKTRDFRTLTRSLTAPEPPTSETALADNACDLRARVALP
                     AGTLYRLVGVGLSGFSEADGNPAMDDLFG"
     gene            complement(7154..8443)
                     /locus_tag="PK399_09030"
     CDS             complement(7154..8443)
                     /locus_tag="PK399_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13300.1"
                     /translation="MSEPTNIDPRLARTAPTWEVELLISGVAIFAMLQLPGWLDDAMF
                     ALEPRLSQEWRLIAVLAYFYSKSAAIVLACTFALHLLLRAHWIALMGVHSVFPRGIHA
                     DNIRMGPIQRALEAAQADDPDDAIERADNRASVVFAIGVSIAGIFAGVCIAFCGTLLV
                     ATLLTNLLGLQVETFVVVGSVFTLLMLPYLLAVTADYYLGERIRPGTRLHRLVSAGLR
                     LYARLGMGRRSNRILATLLSNQGERRTLALVMAIMFLAIASVTATYVAMQASGPIGSY
                     ERFPDARELQVDASHYDDRRDAARDQPVPYIDSMVVEGPYLRLTVPYEPRRDGPAMRS
                     CKQPVHAKSVEQAQALLACLASTRAVLLDGAPIEGLRYEIASDPRTHRPALLAMIDAR
                     ALAPGRHELQVGRPLNPDSRRDRKDPTYRHYRIVFWR"
BASE COUNT         1423 a         2850 c         2945 g         1250 t
ORIGIN      
        1 gatagatggt gtcggtggcg accagcttgc cggtggcgtc gaccaccgcg accttgaccc
       61 cggtgcgcag gccgggatcg aggccgagca cggccttcgg gccggccggc gcggccagca
      121 acaggtcctt gaggttgtcg ccgaagacgt tgatggcttc ggcctcggct ttctcgcgcg
      181 cctggttgaa caggtccagc agcaggtgca tgtggatgcg cgcgcgccaa gcaaggcggc
      241 aggcgttgcg cagccaggca tcggcaggcc ggccgcgttc ggcgatgccg gcatgcaggg
      301 cgatgcggtg ttcggcctgc aggtggccgg cctcggcatc gctgccggga tccaggtcga
      361 gctgcaggaa ttcctcgcgg cggccgcgga acagcgccag caggcgatgc gaggggatct
      421 tcgccagcga ttccacgtgg tcgaagtagt cgcggaattt ttcgccagcg gcttccttgc
      481 cttcgacgac cttcgcgcgg atcaggccgt tgccttccag ccagccgcgc agctcaccga
      541 gcaaggtcgc gtcttccgac cagcgttcga tcaggatcgc gcgcgcgcct tccagcgcag
      601 ccttcgcgtc agccacgccc ttgtccgcat cgatgaacga tgccgcgaag gtgtccggca
      661 gccgcgacgg atcggccagc aggccatcgg ccaagggttg gagcccggcc tcgcgggcga
      721 tctgcgcgcg cgtgcggcgc ttctgcttgt acggcaggta gaggtcttcc agccgcgact
      781 tgctgtcggc ggcgtcgatc tccgcgcgca gtgcgtcggt gagcttgccc tgttcggtga
      841 tgctggcgag gatcgacgcg cggcggtctt ccatctcgcg caggtaggac aggcgcgttt
      901 cgaggttgcg cagctgggtg tcgtccagcc cgccggtcac ttccttccgg tagcgagcga
      961 tgaagggcac ggtcgcgccc tcgtcgagca aagcgaccgc ggcatgcacc tgcgcgtcct
     1021 gtgcgccgat ttcggtggcg atggtctggg cgatcttgcg cgcgagcgcg tctggaacct
     1081 ggggcattcg atggcgggca ctgcggtttc ggagcgcaat tgtcgccgcc gcttcccgtc
     1141 tgcgtaagct tgacaacgaa aaccggggtc agagtcgact ttcaaatctg cgaagcgcag
     1201 ggcatgctca cgctacgaga aaagtcgact ctgaccccgg ttttcattca ccgacgagcg
     1261 gccgccgcat catcacgtgc aagcccaggg ccacgcccag caacgccatc ccggcgatgt
     1321 agtgcatcgg cgcggcgggg ctgtccttca tcgccaggct gagcgcgatt ggcgtcaggc
     1381 cgcccgcgat cgcatacgcc acgttgtacg agaacgacag ccctgagaag cgcacctgcg
     1441 ccggaaagcc gctgaccgcg attgccggta ccagcgcggt cacgcccacg ccgaagcccg
     1501 cgatgacgta cagcgcaggc gaatacccgc cggccaacgc accggcatag aacgtccaga
     1561 acgcgatgcc cagcagcacg ctccacagca ccagcacacg gcccgcaccg aagcggtcgg
     1621 ccatccagcc ggcgaccagg ttggcgatca ccgtcgcgca gatcgccagg gtattgccag
     1681 ccagtgcatc ggcgcggctg acgccgaggc cctgcagcaa ggtcggcatc atcagcagcg
     1741 tcaccaccac cgcggcagtg agcagccagg tcagcagcat cgccagcacg accgcgccgg
     1801 catggtcgcg caccacggcc ttcagcggca attcggccgc aagcgaacgg cgttcgcgca
     1861 tttcggcgaa caccggcgtt tcgtgcagct tgcggcgcag gaacatcgcc agcaggccga
     1921 acacgccgcc ggcgatgaag ggcaggcgcc atgcccacgc cagcacctcg gcttcatcga
     1981 acgcggcatt gatcgcgcgt gccaccaatg agcccagcag gatgccgctg cacaggcctg
     2041 cggagagcgc accacaggcc aaggcgcgat gccgcgtcga cacatgctcg gtgacgaaca
     2101 cccacgcacc cggcgcttcg ccgccgatcg ccgcgccctg catgatccgc agcaccagca
     2161 acaggatcgg cgccagcacg ccgaccgtgg cgtaggtcgg cagcaagccc atcagcaacg
     2221 tggggatggc catcatcagg atgctcagca tgaacatccg cttgcgcccg gacaggtcgc
     2281 cgaagtgcgc catcacgatg ccgcccagcg gccgcgcgag gtaacccgcg gcgaagatgc
     2341 cgaacgtctg caccagcttc agccactcgg gcatgtccgc cgggaagaac agcgcgccca
     2401 tcgtggtcgc gaagaacacg aagatcacga agtcgtagaa ctccagcgca ccgccgagcg
     2461 cggcgagcga gagcgtcctg gcttgctgtc gggtgagtcc gggggcggcg tcggcggtcg
     2521 tcatcgcgct agcgtatgcg atggatggcg acgcgcgaag ccgccgcgag cgtgggctca
     2581 ggccagccag tcgtggagtt gcctggacac ctgcgcactg gcgagcagcc ctaggtggcc
     2641 ggtgcgatgg accagcagcc ggtggtgctc ggggatggcg aggtcgcgcg cgggatccgt
     2701 gtgctcgccg aacgcgctgg cgaccgggac caacccatcc ccatgcaagc gtcgcgcgct
     2761 tgcgtggctg cgcggccccc gggtggaggc ggcgaccgcg aatgcccgca cctgtgccgg
     2821 caatggcgga tggcgaacat gcaggctgcc atggcgcagg tcctggatgc cggcgctgcg
     2881 caggccaccc agggcggtga acggcgcgct gtgggcgctg actcccagca cggtctgcag
     2941 cagggcgccg gcacgctcca gcggtgcgcc caggtgcggc gtacccagtg acaccagcgt
     3001 gtcgagcctg tgcaaccacg ggctgccatc ggcctgcgcg ctcgcgatgg ccgcacgcga
     3061 caccagcccg cccatgctgt gcgcgaggat cgcgatgcgc tgcaccggca ccggccaggc
     3121 atccaccaac gcctgcagca agcgcgaaaa ttcgcggcca ttgtcggcga tcgacaggcc
     3181 actgttgtag tgcaggtgaa tcacttcgaa accgagtgcc tcggcgagct ggttgccatg
     3241 gtgatgcggg ccctgctgcc actgcaggtc gttcatgcac aggccgtgca ccagtaccag
     3301 caggcgcggc ccggtcttcg ggtgggtggc ggccagcgca tcgcgatccg gcaccagcgc
     3361 acgcccgtgc atgcgcagcg tggcggtcaa tgccagcgga ttggcggtgg cgaccagnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc cgtgcatgcg cagcgtggcg
     3541 gtcaatgcca gcggattggc ggtggcgacc aggtggtcgc cgaggacgcc attgagggcc
     3601 gcgatcagcg cttcgcgttg cgggatccgg tcggcctggc cggatcgggt cggcagccca
     3661 cgcaagaccc ggtcgaggcc gttgcccgcc acgcgcgcga tcccccggac cgcgccgtag
     3721 gccagcgccg ggatgccgcc ggtggtcggc ggcggcgaca ggccgagcag tcgccgcggc
     3781 cagccgagga tgctggcatg cacggcttcg accaggtcgg tggtgccggt gaccgcatcg
     3841 accagcaagc cgttggcacc gcgcaggtcg ctgccatcga gcaccagcgg tctcggaagc
     3901 gggtcagggc gcacgggcga gctcatcgtt cgagcgtagc gccgccggct ggagcgaatg
     3961 ttccaaccac cggcggcatt cgccagggca ggcatgccgc atgcgtcgag gccggacgcg
     4021 cgcgcctcgc cctgcctgcg cgcgccgggc gtgcaacact ccgggttcga cggacaacgg
     4081 ggcgtggcga tgcggatcgg gatcgtggtg cggatgacgg ggctggcgct cgcgctggcc
     4141 acggcgggcg gggcgatggc gcagaccgcg ccgatgacac cggacatcac cggcaggaag
     4201 ttcagcgcgc cggagaccca gcgcgatttc atcaagcggg tcgagatggt gccgatgcgc
     4261 gatggcacga agctctacac cgtcatcgtg atcccgaagg gtgcgaagga tgcgccgatc
     4321 ctgttcaccc gcacgcccta caacgcggcc aagcgcgccg atcgcgtgcc caatgccacc
     4381 cgcatgctcg acgcgatgcc gcagggcgac agcgtgttcg tggaggacgg ctacatccgc
     4441 gtgttccagg acgtgcgcgg caaatacggc tcggaaggcg agtacgtgat gacccggcca
     4501 ccgcgcgggc cgctcaacgg cagcggcatc gacaatgcga ccgatgcctg ggacagcatc
     4561 gactggctgg tcaagcacgt gccggaaagc aacggccggg tcggcatgct gggctcgtcg
     4621 tacgaaggct tcaccgtggc gatggcgctg cttgagccgc accctgcact gaaggtcgcc
     4681 gcgccggaaa gcccgatgat cgatggctgg atgggcgacg actggttcca gcacggtgcc
     4741 taccggcaga acagcctggg gtattacacc gggcagatga ccgcgcgcgg cgagggcacc
     4801 agcatcccgc gcgccaccag cgacgactac accagcttcc tgcgtggcgg tgcgctcggc
     4861 gacctggcgc gtgccgccgg actggaacag atcccggcct ggcgcaaggt gatcgagcac
     4921 ccggcctatg accgctattg gcagcagcag gcgctggaca agttgctggc gaagctcccg
     4981 gcgatctccg tgccgacgat gtgggaacag ggcctgtggg accaggaaga catctacggc
     5041 gcgcacaccg cgtgggcggc gctggagccg aaggacaccg acaacacccg caaccacctg
     5101 gtgatcggcc cgtggcggca cagtggcgcc aattacgacg gcagcagcct gggcgacctc
     5161 aggttcgagg gcgataccgc gctgcaatgg cggcgcgagg tgctcaagcc gttcttcgac
     5221 cagtacctca agccgggcgc agcggtggcg aagaccccgc ccgtgtggat ctacgacgcc
     5281 ggcagccagc gctgggacac ctacgcgcag tggccgcaga gttgcgccca gggctgcccg
     5341 aacgccgccg tgccgttgta cctgcatgcc gatgaaaccc tggacggaac ccggcccgtg
     5401 gccgctgggg ccggctacgc cgaatacgtg tccgaccccg ccaagccggt gccgttcgtg
     5461 ccgcgtccgg tgaacatgga cgatcgcccg acctggacca cctggctggt cagggaccag
     5521 cgcttcgtcg atggccgcac cgacgtgctc agctacacca gtgcaccgct ggctaagccg
     5581 ctggcgctgg ccggcattcc cgtcgccaat ctgttcgcgt cgacctcggg cagcgacagc
     5641 gactgggtgg tcaagctgat cgacgtgtac ccagaccagt acccggacga tccgaagatg
     5701 gccggctacc agttgccgct cgccatcacg atcttccggg gccgctatcg cgaggacttc
     5761 gccgcaccga agccgctgca acccggcgtc gcgcaggcct acaccttcga cctgccgacc
     5821 gtgcaccacg tgctcaagcc cggccatcgc ctcatggtgc aggtgcagtc cagcctgttc
     5881 ccgctgtacg accgcaaccc gcagacctac gtcgacaaca tcttcttcgc caagccggcc
     5941 gactatcgca aggccgtcca gcggatcgtc gccacgccgg aacaggcgag tttcatcagc
     6001 ctgccagtgg tgtcggggac attgccctga tcaaccgaaa aggtcatcca tcgccgggtt
     6061 tccatccgct tcgctgaagc cggacaggcc cacgccgacc agccggtaca gcgtgccggc
     6121 gggcagggcc acgcgggcgc gcaggtcgca ggcgttgtcg gcgagcgcgg tttcgctggt
     6181 gggcggctcg ggcgcggtga ggctgcgcgt gagcgtgcgg aaatcgcggg tcttcagttt
     6241 cagcaccacg gtgcgggcga tgcgctcggg gtggcgttcg cgctcacgca ggtagccggc
     6301 ccaggccttg gtcgccaggc gccggatgtg gtcctcgagt gcgtccagcg gcaggtcctc
     6361 ggggaaggtg tcctcggccg agacctgcag ggtcaggcgc tcgggctgca cgctgcgttc
     6421 gtcgatgccc agcgcgagtt catgcaggcg ccggccccaa cgaccgaatc gctgctccag
     6481 cgcggcggcg ccatgcagtc gcaggtcggt gcaggtggcg atgccgagtc cggcgagttt
     6541 cgcttccatc accttgccca cgcccggcag cttgcccacc gcgagggggg cgaggaaggc
     6601 atcgaccatc gccggcttca gcacgaactg cccgtccggc ttgttccagt cgctggcgat
     6661 cttggccagg aacttgttgg gcgcgatgcc ggccgacgcc gtgagttgcg tctcctcgcg
     6721 gatcgcatcg cggatggaca gggcgatcgc ggtggcgctg cccaggtcct gcttcggcgc
     6781 ggtgacatcg aggtaggcct cgtccagcga cagcggttcg atgaggtcgg tgtggcgctc
     6841 gaagatcccg cgcacctgct tcgacaccgc cttgtagcgg atgaagtcgg gcggcacgaa
     6901 gacggcatcc ggacacaagc gttccgcgcg caccgccggc atcgccgaac gcaccccgaa
     6961 cacgcgggct tcgtagctgg ccgcgcagac caccgaacgc gcgccacgcc aggcgacgac
     7021 gaccggcttg ccgcgcaatg aaggatcgtc gcgctgctcg accgacgcat agaacgcgtc
     7081 catgtcgacg tggatgattt tgcgttgcgg cgtgtcgctc acgccgccat tttagatcgc
     7141 gggtatcgca gggtcagcgc cagaacacga tccgatagtg gcgatacgtc ggatccttgc
     7201 ggtcccgcct gctgtccggg ttcaacggcc tgcccacctg caactcatgg cgccctggcg
     7261 ccagcgcgcg tgcatcgatc atcgccaaca aggcaggacg atgggtgcgc gggtcgctgg
     7321 cgatctcgta acgcagtcct tcgatcggcg cgccatcgag caggacggct cgggttgacg
     7381 ccaggcaagc cagcaatgcc tgcgcctgct caacggattt cgcgtggaca ggctgcttgc
     7441 aggaacgcat tgccgggcca tcgcggcgcg gttcgtaggg cacggtcagg cgcaggtagg
     7501 gtccctccac gaccatgctg tcgatgtagg ggacgggctg gtcccgcgcg gcgtcgcgcc
     7561 ggtcgtcgta atgggaggca tcgacctgca gctcacgcgc atcggggaac cgctcatagc
     7621 tgccgatcgg cccgctggcc tgcatcgcga cataggtcgc ggtgacgctg gcgatcgcca
     7681 ggaacatgat ggccatcacc agcgccaggg tgcgccgctc gccctggttg ctgagcaggg
     7741 tggccaggat gcggttgctg cggcggccca tgccgagtct cgcgtagagc cgcaggcccg
     7801 cggacacaag ccggtgcagc cgggtgccgg ggcggatgcg ctcgccgagg taatagtcgg
     7861 cggtgaccgc aagcaggtaa gggagcatca gcagcgtgaa gacgcttccc accaccacga
     7921 atgtttcgac ctgcaggccg agcaggttgg tcaacagggt ggccaccaac aaggtgccgc
     7981 agaacgcgat gcagaccccg gcgaagatcc cggcgatgct gacgccgatc gcgaacacca
     8041 cgctggcgcg gttgtccgcg cgctcgatgg cgtcatccgg atcgtccgcc tgcgcggctt
     8101 cgagcgcgcg ctggatcggc cccatgcgga tattgtccgc gtggattccg cgcggaaaga
     8161 ccgagtgcac gcccatcagc gcgatccagt gggcgcgcag cagcaggtgc agggcgaagg
     8221 tacaggccag cacgatcgcg gcgctcttcg agtaaaagta ggcgagcacc gcgatcagcc
     8281 gccactcctg gctcagccgg ggttccagcg cgaacatcgc atcgtccagc cagccgggta
     8341 gctgcagcat ggcgaagatc gccacgccgg aaatcagcag ttcgacttcc caggtaggag
     8401 ccgtgcgggc aaggcgtgga tcgatgttcg tgggttcgct catggcccgg gatggtgcgg
     8461 gccggtcgcg gatctggcaa gcaggtcaga ccaggctgaa tgcgtagtac agcccgatca
     8521 ccacgaatac cgccatcgtc ttgatgagcg tgaccgcgaa gatgtcct
//
LOCUS       DAOOCX010000093         8544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_7808_length_8544_cov_7.004594, whole genome shotgun sequence.
ACCESSION   DAOOCX010000093 DAOOCX010000000
VERSION     DAOOCX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7808_length_8544_cov_7.004594"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..2575)
                     /gene="mutS"
                     /locus_tag="PK399_09035"
     CDS             complement(11..2575)
                     /gene="mutS"
                     /locus_tag="PK399_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPW13301.1"
                     /translation="MSLEQHTPLMKQFFSAKAEHPDVLLFFRMGDFYELFYDDARKAA
                     RLLDITLTQRGNSAGQPIPMAGVPYHAAEGYLARLVALGESVAICEQIGDPALAKGLV
                     ERKVVRIVTPGTVTDEALLDERRDTLLLAISRTKAGYGLAWADLSSGRLRANEVDGDD
                     ALEAELARLQPAEVLLADEEGWPVAVLESRGLRRRPPWLFDAEAGRRQLLQFFGLHDL
                     TAFGIDGKPRAIAAAAALLGYIEETQKQRLPHLRAIALEASDEAIAMNAATRRHLELD
                     SRIDGDVRATLLGVLDSTVSPMGGRLLRRWLHRPLRDRGVVGQRHHAVSALIDARAGE
                     LLRDAFRSLGDLERILSRVALRSARPRDLSTLRDGLSLLPSVQQILSGLDSPRLQALH
                     AALGEHEATARMLRAAIVPQPPLLARDGGAIADGYDADLDELRQLSTNADQFLVELEA
                     REREATGIATLKVGYNRVHGYYIEISKGQSIKAPVHYTRRQTLTGAERYITEELKAFE
                     DKVLSARERSLSREKLLYEGVLDLLNEVVEPLRHCAAALSELDVLAGFAERAQALDWS
                     MPVLRDEPGIAIERGRHPVVEALRDAPFEPNDLRFDGETRMYVITGPNMGGKSTFMRQ
                     NALIVLLACIGSFVPAAKATIGPIDRILTRIGAGDDLARGQSTFMVEMSETAYILHHA
                     TEHSLVLMDEIGRGTSTYDGLALADAVARQLASANRSYTLFATHYFELTALAEPGSGI
                     ANLHLDAVEHGDALVFMHAVRPGPANRSFGLQVAALAGLPKPALQQARARLAELESQN
                     RDAPTVPLSAPALDAPQQFGLFASRSAALDALAGIDPDELSPKQALEALYRLKSLA"
     gene            2788..5451
                     /locus_tag="PK399_09040"
     CDS             2788..5451
                     /locus_tag="PK399_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006453299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW13302.1"
                     /translation="MYRKQLQPKPLAVAVMAALISLTALPALAQDAAQDKKEEAKTLD
                     ALVVTAQKREEAMQDVPIVVTSLSQQILQDTGVRDIKDLQILVPGLTVTSTQNEAITT
                     ARIRGIGTVGDNVGLESSVGVVIDGVYRPRNSVGFGDLGELERIEVLKGPQGTLFGKN
                     TSAGVINVVTRNPDFELGGAGEVTFGNYGALGLAGWFNAPLGDYAAFRIYAAHRDRDG
                     WMDVVSGAGPRTEDKDYDQNFRTFRGKLLLAPTDNVEIVLSADSTKRDENCCTAVQLV
                     SGSTAAIINGVGGGNAIAVPANPWARVAYSNRDTTQKMNDRGLQAEVNVDLDALGGAT
                     FTSVTGNRDWDAVNGRDFDFTAADLIYQSPEANESFARFKTFSQEFRLAGSTDKVDWL
                     LGFFYANEDLERNESYRVGAGYEPYLSTLVGANALPGLAASLNPILANYGLSFNTSNP
                     ALFLSQFTGKAFGTNFTGLGALDKYRQNAKTTALFTNNTWHATEAFDVTLGLRYTREK
                     KELESLYSNPNGSLGCGTALANPGAAVSRLLAARINGFSQLSPQLQQQLLAGLVPAVA
                     GNQALMSGIIGRACLPWSNALHNGRATDQSRSENEWSGTLKMAYRWNESAMTYFSAAR
                     GYKAGGFNLDRVQSSNGLSSGAAGIVPVDDTSFPGEFVDSYELGAKTTWAGGNLLLNG
                     ALFHQTYSDFQLNSFLGTSFVVRSIPKVTSQGIDAELLWQATPGLMLQTGLVYADTKY
                     GKTIPGADFAAPAGQLYKLPGAQMSFAPKWSLSASMTYEWDIGANMEGRFNIGTKYQS
                     EYNTGSDLDAEKLQDAYAVVNARIGIGARDRKWQVELWGTNILDKDYVQVGFDGPLQA
                     VGSPAPNDPANTYNAFLGAPAMYGMTLRFQF"
     gene            5555..6052
                     /locus_tag="PK399_09045"
     CDS             5555..6052
                     /locus_tag="PK399_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HPW13303.1"
                     /translation="MTVPTDAELDALARSTGERLLATRHRLATAESCTGGWIAKAVTD
                     VAGSSAWFDCGMAAYSYEAKQALLGVRPQTLETHGAVSRETVVEMVSGALVHSGATLA
                     VAVTGIAGPGGGSDDKPVGTVWIAWKRRGGYAKAEVFHFDGDRDAVRRQTVASALAGL
                     EQHLE"
     gene            6106..6741
                     /gene="lexA"
                     /locus_tag="PK399_09050"
     CDS             6106..6741
                     /gene="lexA"
                     /locus_tag="PK399_09050"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011269877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPW13304.1"
                     /translation="MSLTDTQRAIHAFIAERIEGDGFAPSQAEIARAFGFKGVRAAQY
                     HLEALEAAGIVERSPGRARSLRLLQSPDGPQQLPLPANDDVLHLPVLGQVAAGVPIGA
                     DIGSEQVVLMDRAFFTPTPDYLLKVRGDSMRDEGIFDGDLIGVHRTSEARNGQVVIAR
                     IDDEVTVKLLKIAQGRIRLLPRNPDYAPIEVQPGQDFAIEGLYCGLVRPNA"
     gene            6898..7923
                     /gene="recA"
                     /locus_tag="PK399_09055"
     CDS             6898..7923
                     /gene="recA"
                     /locus_tag="PK399_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPW13305.1"
                     /translation="MDENKKRALSAALGQIEKQFGKGSVMRMGDRTVEAVETIGTGSL
                     MLDIALGIGGLPKGRVIEIYGPESSGKTTLTLQTIAECQKAGGTCAFIDAEHALDPVY
                     AQKLGVNLDELYLSQPDTGEQALEIADMLVRSNAVDMVVVDSVAALTPRAEIEGEMGD
                     QLPGLQARLMSQALRKLTGNIKRSNCMVFFINQLRQKIGIMMPGQSNEVTTGGNALKF
                     YASVRLDIRRIGSIKKGEEIIGNQTRIKVVKNKLAPPFKQVITEILYGEGISREGELI
                     DMGVDAKLVEKAGAWYSYDGERIGQGKENARTYLKENPAAALKLEAALREKFVPEEAP
                     REEAGAE"
     gene            7920..8420
                     /locus_tag="PK399_09060"
     CDS             7920..8420
                     /locus_tag="PK399_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPW13306.1"
                     /translation="MTVPGGDGGETSSSARNRRRRPEPSPTQRALGLLTRREHSRKEL
                     ARKLAGRGVETADAHAVIDKLAAAGWQDDSRFARSLVRSRAAVGYGPGWIRAELGTHG
                     LGAEAVSAALGSVEDDWADVARDLVRRRFGDAGLLDIAQRRKAADLLLRRGFSMEHVR
                     AATRHD"
BASE COUNT         1447 a         2849 c         2916 g         1332 t
ORIGIN      
        1 aggggtatgg tcaggccagt gacttcagcc ggtagagcgc ctcgagcgcc tgcttcggcg
       61 acagctcatc ggggtcgatg ccggcgagcg catccagcgc cgccgagcgc gaggcgaaca
      121 ggccgaattg ctgcggtgcg tccagcgccg gcgcactcag cggcacggtg ggcgcatcgc
      181 ggttctgcga ttccagttcg gccaggcgcg ctcgtgcttg ctgcagcgcg ggcttcggca
      241 ggccggcgag tgcggccact tgcaggccga agctgcgatt ggccgggccg ggccgcacgg
      301 catgcatgaa caccagcgcg tcgccgtgtt cgaccgcgtc gaggtgcagg ttggcgatgc
      361 cgctgcccgg ctccgccagg gcggtgagtt cgaagtagtg cgtcgcgaac agggtgtagc
      421 tgcggttggc ggatgccagc tggcgcgcca ccgcatcggc cagcgcaagc ccgtcgtagg
      481 tcgaggtgcc gcggccgatc tcgtccatca gcaccagcga gtgttcggtc gcgtggtgca
      541 ggatgtaggc ggtctcgctc atctcgacca tgaaggtcga ctgcccgcgt gcaaggtcgt
      601 cgccggcgcc gatgcgggtg aggatgcggt cgatcgggcc gatcgtcgcc ttcgccgccg
      661 gcacgaaact gccgatgcag gccagcagca cgatcagcgc gttctggcgc atgaaggtgc
      721 tcttgccgcc catgttcgga ccggtgatca cgtacatccg ggtctcgcca tcgaagcgca
      781 ggtcgttggg ctcgaacggt gcgtcgcgca gcgcctcgac caccggatgg cggccacgct
      841 cgatcgcgat gccgggttca tcgcggagca ccggcatgct ccagtccagc gcctgcgcgc
      901 gctcggcgaa gccggccagt acgtcgagtt cgctcagcgc ggcggcgcaa tggcgcagcg
      961 gctcgaccac ctcgttgagc aggtcgagca cgccttcgta cagcagcttc tcgcgcgaca
     1021 gcgagcgttc gcgcgcggac agcaccttgt cctcgaaggc cttcagttcc tcggtgatgt
     1081 agcgctccgc gccggtcagc gtctgccggc gcgtgtagtg caccggcgcc ttgatgctct
     1141 ggcccttgct gatctcgatg tagtagccgt gcacgcggtt gtagccgacc ttcagggtgg
     1201 cgatgccggt ggcttcgcgc tcacgcgctt ccagctcgac caggaactgg tcggcgttgg
     1261 tcgacaactg gcgaagctcg tcgaggtcgg cgtcgtagcc gtcggcgatg gcgccgccgt
     1321 cgcgggcaag caacgggggt tgcgggacga tggccgcgcg cagcatgcgc gcggtcgcct
     1381 cgtgttcgcc cagggctgcg tgcagcgcct gcaggcgcgg cgaatccagt ccggacagga
     1441 tctgctgcac cgagggcagc agcgacaagc cgtcgcgcag cgtggagagg tcgcgcgggc
     1501 gcgcgctgcg cagggcgacc cgcgacagga tgcgttccag gtcgccgagg ctgcggaagg
     1561 cgtcgcgcag cagctcgccg gcgcgcgcat cgatcaaggc cgacaccgca tggtggcgct
     1621 ggccgacgac gccgcggtcg cgcaagggcc gatgcagcca gcgccgcagc aggcgcccac
     1681 ccatcggcga cacggtggaa tcaagcacgc cgagcagggt cgcgcgcacg tcgccatcga
     1741 tgcgcgaatc cagttccagg tggcggcgcg tggccgcgtt catggcgatg gcctcgtcgc
     1801 tggcctcgag tgcgatcgcg cgcaggtgcg gcaggcgctg cttctgggtt tcctcgatgt
     1861 agcccagcag cgccgcggcg gcggcgatcg cgcgcggctt gccatcgatc ccgaaggcgg
     1921 tgaggtcatg caggccgaag aactgcagca actggcggcg gccggcctcg gcatcgaaca
     1981 accacggcgg gcgacggcgc aggccgcgcg attccagcac cgcgacgggc cagccttctt
     2041 cgtcggccag caggacttcg gctggctgca ggcgggccaa ctcggcttcc agtgcatcgt
     2101 cgccatcgac ttcattggcg cgaaggcgac cgctggacag gtccgcccag gccagcccgt
     2161 atcctgcctt cgtgcggctg atcgccagca gcagggtgtc acggcgttcg tcgagcaggg
     2221 cttcgtcggt caccgtgccg ggggtgacga tacgcacgac cttgcgctcg accaggccct
     2281 tggccagtgc cgggtcgccg atctgctcgc agatcgccac cgattcgccc agcgccacca
     2341 gccgcgccag gtagccttcg gccgcgtggt agggcacgcc ggccatcggg atcggttggc
     2401 cagcggagtt gccgcgctgg gtcagggtga tgtcgagcag gcgcgcggcc ttgcgggcgt
     2461 cgtcgtagaa cagttcgtag aaatcgccca tccggaagaa caacagcacg tccggatgct
     2521 cggccttggc cgagaagaat tgcttcatca acggggtgtg ctgttcgagc gacatggggg
     2581 gtgcgtgggc agggggcggc tagtgtgccc gatcctccca aggccgccgc aattcactgg
     2641 acacgccggc gtatggatgt gttagttttc cgtcaaggca gcgttcaact gcccgcgcgc
     2701 cataccccca catggtcgcg ccaacgaaaa tgcagccgcc gcgcccgcga cggctggaat
     2761 tcaggcgtgt tcaacctagg gggttccatg tatcgcaagc aactgcagcc gaagccgttg
     2821 gccgtcgccg tgatggcggc attgatttcg ctcaccgcct tgcccgcgct tgcccaggat
     2881 gcagcgcagg acaagaagga agaagcgaag accctggacg ccctggtcgt gaccgcgcag
     2941 aagcgcgagg aagcgatgca ggacgtgccg atcgtcgtga ccagcctgtc gcagcagatc
     3001 ctgcaggaca ccggcgtgcg cgacatcaag gacctgcaga tcctggtgcc gggcctgacg
     3061 gttaccagca cccagaacga ggccatcacc acggcgcgca tccgcggcat cggcaccgtg
     3121 ggcgacaacg tcggcctgga gtcctcggtc ggcgtggtca tcgacggcgt gtatcgcccg
     3181 cgcaactccg tgggtttcgg cgacctgggc gaactcgagc gcatcgaagt gctgaagggc
     3241 ccgcagggca ccctgttcgg caagaacacc tcggccggcg tgatcaacgt ggtcacccgc
     3301 aatcccgact tcgaactcgg cggcgccggt gaagtgacct tcggcaacta tggcgcgctg
     3361 ggcctggccg gctggttcaa tgccccgctg ggcgactatg ccgcgttccg catctacgcg
     3421 gcgcaccgcg accgcgacgg ctggatggac gtggtgagcg gcgccggccc gcgcaccgag
     3481 gacaaggact acgaccagaa cttccgcacc ttccgcggca agctgctgct ggcgccgacc
     3541 gacaacgtcg agatcgtcct gtccgccgac agcaccaagc gcgacgagaa ctgctgcacc
     3601 gccgtgcaac tggtcagcgg cagcaccgcc gcgatcatca acggcgtggg cggcggcaac
     3661 gcgatcgcgg tcccggccaa tccgtgggcg cgggtggcgt acagcaaccg cgataccacg
     3721 cagaagatga acgaccgcgg gctgcaggcc gaggtcaatg tcgacctgga tgcgcttggc
     3781 ggcgccactt tcacctcggt gaccggcaat cgcgactggg acgcggtcaa cggacgcgac
     3841 ttcgacttca ccgccgccga cctgatctac cagagccccg aagccaacga gtcgttcgcg
     3901 cgcttcaaga ccttcagcca ggagttccgc ctggccggtt cgaccgacaa ggtcgactgg
     3961 ttgctggggt tcttctacgc caacgaggac ctggagcgta acgagagcta tcgcgtgggc
     4021 gcgggttacg agccctacct ctcgaccctg gtcggcgcga atgcgctgcc tggcctggcg
     4081 gcctcgctga acccgatcct ggccaactat ggcctgtcgt tcaacacgtc caacccggcg
     4141 ctgttcctgt cccagttcac cggcaaggcc ttcggtacca acttcaccgg cctcggcgcg
     4201 ctcgacaagt acaggcagaa cgccaagacc acggcgctgt tcaccaacaa cacctggcac
     4261 gccaccgagg cgttcgacgt gacccttggc ctgcgctaca cccgcgagaa gaaggaactc
     4321 gagtccctct atagcaaccc gaacggtagc ctgggctgtg ggacggcgct ggccaacccc
     4381 ggtgccgcgg tgtcgcggct gctggcggcg cgcatcaacg ggttctcgca gctgtcgccg
     4441 cagttgcagc agcagctgct ggccgggctg gtgcccgcgg tggccggcaa ccaggcgctg
     4501 atgtccggca tcatcggccg cgcctgcctg ccgtggagca atgcgctgca caacgggcgc
     4561 gccaccgacc agtcccgttc cgagaatgaa tggtccggca ccctgaagat ggcctatcgc
     4621 tggaacgaga gtgcgatgac ctacttctcg gccgcccgcg gttacaaggc aggcgggttc
     4681 aacctggatc gcgtgcagtc gtcgaatggc ctgtccagcg gcgccgccgg catcgtcccg
     4741 gtcgatgaca cctcgttccc gggcgagttc gtcgacagct acgagctggg cgccaagacc
     4801 acctgggccg gcggcaacct gctgctcaac ggcgcgctgt tccaccagac ctacagcgac
     4861 ttccagctca acagcttcct gggcaccagc ttcgtggtgc gctcgatccc gaaggtgacc
     4921 tcgcagggca tcgacgccga gctgctgtgg caggccacgc ccggcctgat gctgcagacc
     4981 ggcctggtct atgccgacac caagtacggc aagaccatcc cgggtgccga cttcgcggcg
     5041 ccggccggcc agttgtacaa gctgccgggc gcgcagatga gctttgcgcc gaagtggtcg
     5101 ctgtcggcat cgatgaccta cgaatgggac atcggcgcga acatggaagg ccgcttcaac
     5161 atcggcacca agtaccagtc cgaatacaac accggctccg acctggacgc cgagaagctg
     5221 caggacgcgt acgccgtggt caacgcgcgc atcggcatcg gcgcgcgcga ccgcaagtgg
     5281 caggtggaat tgtggggcac caacatcctc gacaaggatt acgtgcaggt cggtttcgac
     5341 ggtccgttgc aggccgtcgg ctcgccggcg ccgaacgatc cggcgaacac ctacaacgct
     5401 ttcctggggg ccccggcgat gtatggcatg accctgcgct tccagttctg atcccgggcg
     5461 atcgaagcga tccacggacg gcccgcgcaa gcgggccgtt ccgttttgcg acgtggtgcg
     5521 ctacaagtcg gccatcccca gcgatgcgtg cacgatgacc gtacccaccg acgccgaact
     5581 cgacgccctc gcacgcagca ccggcgagcg actgctggcc acgcgccacc ggctggccac
     5641 cgcggaaagc tgcaccggtg gctggatcgc caaggcagtg accgatgtcg ccggctcgtc
     5701 cgcctggttc gattgcggca tggccgctta cagctacgag gccaagcagg ccttgctggg
     5761 cgtgcgcccg caaaccctgg aaacgcacgg cgcggtcagt cgcgaaacgg tggtggagat
     5821 ggtgagcggc gcactcgtgc attccggcgc gaccctcgcg gtggcggtca ccgggatcgc
     5881 cgggccggga gggggcagcg acgacaagcc cgtcggcacg gtctggatcg cctggaagcg
     5941 gcgcggtggt tacgccaagg cggaggtctt ccatttcgat ggcgaccggg atgccgtccg
     6001 gcggcagacc gtggccagcg cactcgccgg cctggaacaa cacctcgagt aaatcttcgc
     6061 ctcgacggtt gacgctccga ttcgttagta gtattactac taaccatgag cctgaccgat
     6121 acccaacgcg ccatccatgc cttcattgcc gagcggatcg agggcgacgg gttcgccccc
     6181 tcgcaggcgg aaatcgcccg cgcgttcggc ttcaaggggg tccgcgccgc gcaataccac
     6241 ctggaggcac tggaagccgc cggtatcgtc gaacgcagtc cggggcgcgc ccgcagcctg
     6301 cgcctgctgc agtcgccgga tggcccgcag caactgccat tgcccgccaa cgatgatgtc
     6361 ctgcacttgc ccgtcctggg acaggtcgcc gctggcgtgc cgatcggggc ggacatcggc
     6421 tcggagcagg tggtgctgat ggatcgcgcg ttcttcacgc ccacgccgga ctacctgctc
     6481 aaggtgcggg gtgattcgat gcgcgacgaa ggcatcttcg acggcgacct gatcggtgtg
     6541 catcgcacct ccgaggcgcg caacgggcag gtggtcatcg cccgcatcga cgacgaggtc
     6601 acggtcaagt tgctgaagat cgcgcagggc cgcattcgcc tgctgccgcg caatcctgac
     6661 tacgcgccga tcgaggtcca gccaggccag gatttcgcca tcgagggcct gtactgcggc
     6721 ctggtgcggc cgaatgcgtg agcccccgag ccgccggcag gggattccct gatggccgcc
     6781 catcgtgccg cctgccactg tcatcgcacg gcccgccacg tcgcagcttt tgcgatgggt
     6841 gccgcctaac ctgatcccag acccgatatc accccttcag taccgaggac ctgcaccatg
     6901 gacgagaaca agaagcgcgc cctttccgcc gcactgggcc agatcgagaa gcagttcggc
     6961 aagggctcgg tcatgcgcat gggcgaccgc acggtggagg cggtcgagac catcggtacc
     7021 ggctcgttga tgttggacat cgcgctgggc atcggcggcc tgcccaaggg tcgtgtgatc
     7081 gagatctacg ggccggaatc gtccggcaag accacgctca ccctgcagac catcgccgag
     7141 tgccagaagg ccggtggtac ctgcgccttc atcgacgcgg agcacgcgct ggatccggtc
     7201 tacgcgcaaa agctgggcgt caacctggac gagctctacc tgtcgcagcc ggataccggc
     7261 gagcaggcgc tggagatcgc cgacatgctg gtccgttcca acgcggtgga catggtggtg
     7321 gtcgactcgg tcgccgcgct gaccccgcgc gccgagatcg agggcgagat gggcgaccag
     7381 ctccccggcc tgcaggcgcg cctgatgagc caggcgctgc gcaagctgac cggcaacatc
     7441 aagcgcagca actgcatggt gttcttcatc aaccagctgc gccagaagat cgggatcatg
     7501 atgcccggcc agagcaacga agtgaccacc ggcggcaacg cgctgaagtt ctatgcctcg
     7561 gtgcgcctgg acatccgccg catcggctcg atcaagaagg gcgaggagat catcggcaac
     7621 cagacccgga tcaaggtggt caagaacaag ctggcgccgc cattcaagca ggtcatcacc
     7681 gagatcctct acggcgaggg catcagtcgc gagggtgagc tgatcgacat gggcgtggac
     7741 gccaagcttg tcgagaaagc cggtgcctgg tacagctacg acggcgagcg catcggccag
     7801 ggcaaggaaa acgcccgcac ctacctcaag gagaatcccg ccgcggcact gaagctggaa
     7861 gccgcgctgc gcgagaagtt cgtgcctgaa gaggcaccgc gcgaagaggc aggcgccgaa
     7921 tgacggtgcc gggcggcgat ggcggtgaaa cgtcctcgtc agcgcggaat cgccgtcgcc
     7981 gcccggagcc ttcacccaca cagcgggcgt tgggtctgtt gacccgccgc gagcattcgc
     8041 gcaaggaact ggcgcgcaag ctcgctggcc gtggcgtcga gaccgcggac gcacatgcgg
     8101 tcatcgacaa gctggccgcc gccggctggc aggacgacag ccggttcgcc cgatcgctcg
     8161 tacgcagccg cgctgccgtt ggctatgggc ctggctggat ccgggcggag ctcggcacgc
     8221 acggtttggg tgctgaagcg gtctcggccg ccttgggcag tgtcgaggac gattgggccg
     8281 atgtggcccg tgacctggtc cgtcgccgtt tcggcgatgc cggcctgctg gacatcgcgc
     8341 agcgacgcaa ggcggcagac ctgctgctgc gcagggggtt ctcgatggaa catgtgcggg
     8401 cggcaactcg ccacgactaa cgtgttcccc ggggcctggc ggcacctcga cgacggacat
     8461 gcgatttaga gccttgcagg cccgtcgccc ctgtaaaatg gccggatgaa aacgacttcc
     8521 gatatccgcc gggacttcct cgag
//
LOCUS       DAOOCX010000094         8354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_8033_length_8354_cov_9.217617, whole genome shotgun sequence.
ACCESSION   DAOOCX010000094 DAOOCX010000000
VERSION     DAOOCX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8033_length_8354_cov_9.217617"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PK399_09065"
     CDS             complement(<1..266)
                     /locus_tag="PK399_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW13307.1"
                     /translation="MANPFSLEGKVALVTGANTGLGQGIALALAEAGADIAGAGIVPA
                     DETAEKVRALGRRFVNIEANLMSIEPVGRIVEETVAGLGGLDILV"
     gene            complement(336..1184)
                     /gene="kduI"
                     /locus_tag="PK399_09070"
     CDS             complement(336..1184)
                     /gene="kduI"
                     /locus_tag="PK399_09070"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HPW13308.1"
                     /translation="MFSKTYHATHPDMMSGASNDQLRDRYLVGEMFVADDIRLSYSHN
                     ERFVIGGAAPVGQAVVLPKQAEPASAAGHPFLERRELGVINVGGGSGTVTVDGATFAL
                     GPKDGLYIAMGSVDVVFASDDAANPAKFYLASTPAHARFETKQLSIKDAVALERGALE
                     TSNERTIYQFIVPATCQSSQLLLGLTVLKPGSVWNTMPPHLHDRRSEIYFYFDLGAND
                     RVYHFMGEPDAQRHIVVQNDEAVISPPWSIHMGSGTSNYAFIWAMGGENLDYTDMHVL
                     DICQLK"
     gene            complement(1318..2370)
                     /locus_tag="PK399_09075"
     CDS             complement(1318..2370)
                     /locus_tag="PK399_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW13309.1"
                     /translation="MTVRSKSAGSESAASGKASTINDIARLSGVSKKTVSRIINDSPL
                     VRKDTRERVEAVMREVGYSPDPMARGLAFRRSFLIGMVYDNPTAQYIVNMQYGALDAL
                     RDTPFELVVHPCDSRSPGYIEGVRRFVQQQKLHGVMLVPRASEDQALADMLADIDCRY
                     TRIASVAMDEPARMVVTHDRNGAAEVADYLLGLGHTDIALITGPAAYRSAIERTQGFI
                     EAMASKGVQVPKARVVEAGYTFESGVAAAEKLLFGKQRPTAIFCGNDEMAAGVFKVAM
                     RAGINIPRQLSVVGFDDSPLASRLWPSLTSVRRHTRDTGRIAAAMLIQPEAAVALSAA
                     SIRPHLIIRDSAQPPE"
     gene            complement(2459..3742)
                     /locus_tag="PK399_09080"
     CDS             complement(2459..3742)
                     /locus_tag="PK399_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPW13310.1"
                     /translation="MAISLLFLVFALLLVIGVPVAFALAASALATLLYLGLPPVVLVQ
                     QISAGTGSASLIAIPLFIFAGEIMMRGGISERLIALAASLVGRLRGGLGQVSILSSLF
                     FGGVSGSAIADVSAVGGTMIPQMVKRGYDRDFAVNVSVTAALVALLVPPSHNLILFSA
                     AAGGGLSIADLFAAGIVPALLMTVVLMVTGYVVARRRGYGVEAFPGWRAVATRTVSAL
                     PGLGLVTLIFVGIRAGIFTAVESAAIAVVYALLVTSVLYRQLDVREFLATLVHAARST
                     GVILFVIATAAVFGWLLAYLQVPAAAVEFLQSFAHGKAMVLLMIIVMLLLLGTFMDLA
                     PLILICTPIFLPVAKAYGLDPIHFGLVLVLAGGIGLVTPPVGSVLFIGTAIGKITIAQ
                     SLRTIWPFWLAGVVVLLLVAFVPELSMWLPKALKA"
     gene            complement(3742..4284)
                     /locus_tag="PK399_09085"
     CDS             complement(3742..4284)
                     /locus_tag="PK399_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HPW13311.1"
                     /translation="MSAEMTAPPATAAQRLIGRIAGLAIGIAATALFGLVVVQGWQVF
                     ARYVINDSPSWTEPVTLLLLATATSLGAACGVHTHRHFGFFLLHDHVRPGMRRAIDVI
                     VTVVIVAIGAVLAYWGAVLLLDGLDIKAAGADLPQSINYLPLSIGGALMVVFALNRLV
                     LAFGPQEPAAVAPAPQEGDL"
     gene            complement(4304..5320)
                     /locus_tag="PK399_09090"
     CDS             complement(4304..5320)
                     /locus_tag="PK399_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPW13312.1"
                     /translation="MSGISRRRLLFAGAGAACASLAAGTPLFAGAARAASGDRLLTAT
                     DVHVKDYPTVEAVRWIGETLERETGGRLKLRLYHSGQLGRESEAIDMARFGAIDITRV
                     YSGALNNAFPLTRALCLPYVFDSVAHMRRALDGGLADAVLDGFAARDLVGLAVYDSGA
                     RCFYNTKHPIVTPKDLHGLKLRVANSDIFIELVRLLGANPTPMSLGDTFSGMETHMID
                     GAENNMRSFHSSRHFEAAKFWSQTEHSYAPDILLLSRRTYDELAPDDRALLRDTARAS
                     VVVMRAKWDESEGKARQAVLDAGIRSNEVDMAAFRDVARPLRDRYLQQPEIQTLYRRI
                     RDVA"
     gene            complement(5317..6147)
                     /locus_tag="PK399_09095"
     CDS             complement(5317..6147)
                     /locus_tag="PK399_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HPW13313.1"
                     /translation="MDNSNDTTDAARSAIVGAFLQARRDGASLAEFPGEIPHDLVAAY
                     EVQDLAISQWPDQVVGWKVGYIAPDRRDASGDERLLGPIFARQFWTATGGIAPIPVFA
                     GGFAAIEAEYVLRLDADAPADQLEWTADEAAALPATLFLGLEVASSPLATINVLGPRV
                     VVSDFGNNNGLLLGAEVPDWTARDEDGLVAETFIEGTSVGTGGACRLPGGLRGAFAFA
                     LARSARRGRPLRRGEWVATGNATGIHDIEVGQHGKVVFAGLGALEVVATAAAPDGHAG
                     "
     gene            6311..7651
                     /locus_tag="PK399_09100"
     CDS             6311..7651
                     /locus_tag="PK399_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13314.1"
                     /translation="MIRTVPCIRPVLGATLAALLGVASLHAMAADSAIPAFPGAMGWA
                     AQTPGGRGGRIIKVTTLAPTGPGSLREALDAKGPRIVVFEVGGVIDLGVETVKISEPF
                     LTIAGQTAPQPGITLIRGGIDITAHDVIVQHIRVRPGDAGLPKMGGNDFDAISTVGAY
                     NVIVDHCSLTWASDENLSASGTRFKGDTPEQWRAGTSHRITFSNNIIAEGLAHANHIK
                     GEHSKGSLIHDNARDILIVGNLYAQNVERNPFFKGGTQGWVVNNLIYNPGQRAIHYNL
                     IAAEWAGHPYQAGRLAVIGNVMHAGPDTPANAAFFMFGGSGELDLYLHDNIAVDRIGN
                     PIPMQGRYTTAPVAPRMLKAAPPMPFGVEPLPANQVQDAVIANVGARPWDRDDIDRRI
                     LENTIEGRGHIIDSQDEVGGYPRHAETRQAFVPADWNLDTMEPLKPLKQRDPLR"
     gene            7659..>8354
                     /locus_tag="PK399_09105"
     CDS             7659..>8354
                     /locus_tag="PK399_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HPW13315.1"
                     /translation="MDRQRRDLLKASLALGLAAAAPAFAAPRLERSSARTRSGHVLGL
                     RNGDLHVFRGIRYGADTSTHRFQPPQRELPWRGAREALAFGASAPQGGGDGPGSEDCL
                     FLNLTTPGLRDGRKRPILFYIHGGGFNNGSGSDPLYDGSQLCTHGDVVVVTVNHRLNA
                     FGYLYLGEFDQRFAESGNVGQLDLIAALQWVREHAAEFGGDAGNVTVFGQSGGGAKIA
                     TLMAMPAARGLFHR"
BASE COUNT         1418 a         2897 c         2828 g         1211 t
ORIGIN      
        1 accaggatgt cgagaccgcc gaggccggcg acggtttcct cgacgatgcg gccaaccggc
       61 tcgatgctca tcaggttggc ctcgatgttg acgaagcggc ggccgagcgc gcgcaccttc
      121 tccgcggttt catccgccgg gacgatgccg gcaccggcga tgtccgcgcc ggcttccgcc
      181 agcgccaacg cgatgccctg gccgaggccg gtgttggcgc cggtgaccag cgcgaccttg
      241 ccttcgagac tgaacggatt tgccatggcg acgcttcccc tttccattgc ctgtggtgag
      301 ccggaccgcg gcgcggtcgc ggcgatgcgc ggtgcttact tgagttggca gatgtccagc
      361 acgtgcatgt cggtgtagtc caggttctct ccgcccatcg cccagatgaa ggcgtagttg
      421 ctggtgcccg accccatgtg gatcgaccac ggcggcgaga tcaccgcctc gtcgttctgc
      481 accacgatgt ggcgctgcgc gtccggctcg cccatgaagt ggtagacgcg gtcgttggcg
      541 cccaggtcga agtagaagta gatctcgctg cgacggtcgt gcaggtgcgg cggcatggtg
      601 ttccacacgc tgcccggctt gagcacggtc aggcccagca gcagctgcga ggactggcag
      661 gtggccggca cgatgaactg gtagatggtc cgctcgttgc tggtttccag cgcgccgcgc
      721 tccagcgcca ccgcatcctt gatcgacagc tgcttcgtct cgaagcgcgc atgcgcgggc
      781 gtggaggcca ggtagaactt cgccgggttg gcggcatcgt cggaggcgaa caccacgtcg
      841 acgctgccca tggcgatgta caggccatcc ttcggtccca gcgcgaacgt cgcgccatcc
      901 acggtcaccg tgccgctgcc gccgccgaca ttgatcacgc ccaactcgcg acgctccagg
      961 aacggatggc ctgcggcgga tgcgggctcg gcctgcttcg gcagcacgac ggcctggccc
     1021 acgggcgccg cgccaccgat cacgaagcgc tcgttgtgcg aatagctcaa gcggatgtcg
     1081 tcggccacga acatctcgcc gaccaggtag cggtcgcgga gctggtcgtt gctggcgccg
     1141 gacatcatgt ccggatgggt ggcgtggtag gtcttgctga acatgcaggt gactccggcg
     1201 gtgctgcgtg gtgcccgggg atgggcagcg gacgtggggt ttcggggatt ctagcgagtt
     1261 ctggacaccg gtgtcatttt gcgctgcggc acaagccggt aacgcaacca gcgtgggtca
     1321 ttcgggtggc tgggcggagt cgcggatgat caggtgcggg cggatgctgg ccgccgacaa
     1381 cgcgaccgcg gcctccggct ggatcagcat cgccgcggcg atgcggccgg tgtcgcgggt
     1441 atgccggcgc accgaggtca gcgacggcca caggcgcgag gcgagcgggc tgtcgtcgaa
     1501 ccccaccacc gagagctggc gcgggatgtt gatgccggca cgcatggcga ccttgaacac
     1561 gccggcggcc atttcgtcgt tgccgcagaa gatcgcggtg ggacgctgct tgccgaacag
     1621 cagcttctcc gccgcggcca cgccggattc gaaggtgtag cccgcctcga ccacgcgcgc
     1681 cttcggcacc tgcacgccct tgctggccat cgcctcgatg aagccctggg tgcgctcgat
     1741 cgccgagcga taggccgccg ggccggtgat cagggcgatg tcggtgtggc ccagcccaag
     1801 caggtagtcg gcgacttcgg ccgcgccgtt gcggtcgtgg gtgaccacca tccgcgcggg
     1861 ttcgtccatc gccaccgagg cgatgcgcgt gtagcggcaa tcgatgtcgg ccagcatgtc
     1921 ggccagggcc tggtcttccg acgcacgcgg caccagcatc accccgtgca gcttctgctg
     1981 ctggacgaag cggcgcacgc cctcgatgta gccggggctg cggctgtcgc aggggtgcac
     2041 gaccagttcg aacggcgtat cgcgcaacgc atccagcgcg ccgtactgca tgttgacgat
     2101 gtactgcgcg gtcgggttgt cgtagaccat gccgatcagg aacgagcggc ggaacgccag
     2161 cccgcgcgcc atcggatccg gcgagtagcc gacctcgcgc atcaccgcct cgacccgctc
     2221 gcgggtgtcc ttccggacca gcggcgagtc gttgatgatc cgcgacaccg tcttcttcga
     2281 cacccccgac aggcgggcga tgtcgttgat cgtggacgcc ttgcccgatg ctgcgctttc
     2341 ggaaccggcg gatttactgc ggacggtcat ggacggttcg ggctcttcga aggcgatgga
     2401 cgtggttcga ttctagcgac cggcgtcccc gccagggcgc aagccggccc gtgcatgctc
     2461 atgccttcag cgccttcggc agccacatcg acaactcggg cacgaaggcg accagcagca
     2521 gcacgaccac gccggccagc cagaacggcc agatcgtgcg caggctctgg gcgatggtga
     2581 tcttgccgat cgcggtaccg atgaacagca ccgaccccac cggcggggtc accaggccga
     2641 tgccgccggc cagcaccagc accaggccga agtggatcgg atcgaggccg taggccttgg
     2701 ccaccggcag gaagatcggc gtgcagatca ggatcagcgg cgccaggtcc atgaacgtgc
     2761 ccagcaacag cagcatcacg atgatcatca gcaggaccat cgccttgccg tgggcgaacg
     2821 actgcaggaa ctcgaccgcg gccgccggca cctgcaggta ggccagcagc cagccgaaca
     2881 ccgccgcggt ggcgatcacg aacaggatca cgccggtgct gcgcgcggca tgcacgaggg
     2941 tggccaggaa ctcgcgcacg tccagctggc gatacagcac gctggtcacc agcagggcgt
     3001 acaccaccgc gatcgccgcc gattccaccg ccgtgaagat gccggcgcgg atgccgacga
     3061 aaatcagcgt gaccaggccc aggccgggca gcgcggacac cgtgcgcgtc gccaccgcgc
     3121 gccagcccgg gaacgcctcc acgccgtagc cgcgacggcg cgccaccacg tagccggtga
     3181 ccatcagcac caccgtcatc agcagcgccg gcacgatacc ggccgcgaac aggtcggcga
     3241 tcgaaagacc gccaccggcc gctgcggaaa agaggatcag gttgtgcgat ggcggcacca
     3301 gcaacgcgac cagcgcggcg gtcacgctga cattgaccgc gaagtcgcgg tcgtaaccgc
     3361 gcttgaccat ctgcgggatc atcgtgccgc cgaccgcgga aacgtcggcg attgcggacc
     3421 cggacacccc gccgaaaaac agcgaggaca ggatcgacac ctgccccagg ccgccgcgca
     3481 ggcggccgac cagcgacgcg gccagcgcga tcaggcgttc ggaaatgccg ccgcgcatca
     3541 tgatctcgcc ggcgaagatg aacagcggga tcgcgatcag cgacgccgag ccggtgccgg
     3601 cggaaatctg ctggaccagc accaccggcg gcaggccgag gtacagcagg gtggccaacg
     3661 cggaagccgc cagcgcgaac gccaccggca cgccgatgac cagcagcaag gcgaacacca
     3721 ggaagagcag ggagatcgcc atcagagatc cccttcctgc ggcgcgggcg cgaccgctgc
     3781 cggttcctgc gggccaaacg ccaggaccag ccggttgagc gcgaacacca ccatcaacgc
     3841 gccgccgatc gagagcggca ggtagttgat gctctgcggc aggtcggcgc ccgcggcctt
     3901 gatgtccagc ccgtcgagca gcagcaccgc accccagtaa gcgagcaccg caccgatcgc
     3961 cacgatcacc acggtcacga tcacgtcgat ggcgcggcgc atgcccggcc ggacgtggtc
     4021 atgcagcagg aagaagccga aatggcgatg ggtgtgcacc ccgcaggcgg caccgaggct
     4081 ggtggcggtg gccagcaaca gcaaggtcac cggctcggtc cagctgggcg aatcgttgat
     4141 cacgtaacgg gcgaacacct gccagccctg caccaccacc agcccgaaca gggcggtggc
     4201 ggcgatgccg atggcaaggc cggcgatccg gccaatcagg cgttgggcgg cggtggccgg
     4261 gggcgcggtc atctctgcgg acatccgtgt attcctgggc tgctcaggcg acgtcgcgga
     4321 tgcgacggta gagggtttgg atctcgggct gctgcaggta gcggtcgcgc agcgggcgag
     4381 cgacgtcgcg gaacgcggcc atgtcgacct cgttgctgcg gatgccggca tcgagcaccg
     4441 cctggcgcgc cttgccctcc gattcgtccc acttcgcgcg catcaccacc accgaggcgc
     4501 gcgcggtgtc ccgcagcagc gcgcgatcat cgggagcaag ttcgtcgtac gtgcgccgcg
     4561 agagcagcag gatgtcgggt gcgtaggaat gttcggtctg cgaccagaac ttcgcggcct
     4621 cgaagtggcg gctggagtgg aagctgcgca tgttgttctc cgcaccgtcg atcatgtgcg
     4681 tctccatgcc ggagaaggtg tcgcccagcg acatcggcgt ggggttggcc ccgagcaggc
     4741 gcaccagctc gatgaagatg tcggaattgg ccacgcgcag cttcaggcca tgcaggtcct
     4801 tcggcgtcac gatcgggtgc ttggtgttgt agaagcaacg cgcaccgctg tcgtacaccg
     4861 ccagcccgac caggtcgcgc gccgcgaaac cgtccagcac cgcgtcggcc aggccgccat
     4921 ccagcgcgcg gcgcatgtgt gcgaccgaat cgaacacgta cggcaggcac agcgcacggg
     4981 tcagcgggaa tgcgttgttc agcgcacccg aatacacgcg ggtgatgtcg atcgcgccga
     5041 agcgggccat gtcgatggct tcggactcgc ggccgagctg gccggagtgg tacagccgca
     5101 acttcaggcg gccgccggtc tcgcgttcga gggtctcgcc gatccagcgc accgcctcga
     5161 ccgtcgggta gtccttgacg tgcacgtcgg tggcggtgag caggcgatcg cccgatgccg
     5221 cacgtgcagc gcccgcgaac aatggcgtgc cggcggccag gctggcgcag gcggcgccgg
     5281 caccggcgaa cagcagccgt cgacgactga tcccgctcat cccgcgtgcc cgtccggcgc
     5341 ggcggcagtc gccaccacct ccaatgcgcc caatcccgcg aacaccacct tgccgtgctg
     5401 gccaacctcg atgtcgtgga tgccggtggc attgccggtg gcgacccatt cgccacggcg
     5461 caatggccgc ccacgccggg ccgaacgtgc caatgcgaac gcgaacgccc cgcgcaggcc
     5521 acccggtagg cggcaggcac cgccggtgcc gacgctggtg ccttcgatga aggtctcggc
     5581 gaccaggcca tcctcgtcgc gcgcggtcca gtccggcact tccgcgccaa gcagcaggcc
     5641 gttgttgttg ccgaaatcgg acaccaccac gcgcggcccg agcacattga tcgtcgccag
     5701 cgggctgctg gccacctcca gccccaggaa cagggtggcg ggcaaggccg ccgcttcgtc
     5761 cgccgtccac tccagctgat cggccggggc gtcggcgtcc aggcgcagca cgtattcggc
     5821 ctcgatcgcg gcgaacccgc cggcgaacac cgggatcggc gcgatgccgc cagtggccgt
     5881 ccagaactgg cgggcgaaga tcgggccgag caggcgctca tcgccggagg catcgcggcg
     5941 gtctggcgcg atgtaaccca ccttccagcc caccacctgg tccggccact ggctgatcgc
     6001 caggtcctgc acctcgtagg cggcgaccag gtcatgcgga atctcaccgg ggaattccgc
     6061 cagcgacgcg ccatcccggc gtgcctgcag gaatgccccg acgatcgcgc tgcgcgccgc
     6121 gtccgtcgtg tcgttgctgt tgtccatgcc caatccttga ctccccatgt tgcggtgcag
     6181 atcgccgacc gccggagttc ggtataaggt aaaccggtgt cattgacaat gtgcactgcc
     6241 gcaaaaatgc tgccgcccgg caccgatccc acaccatgag cctgccaatc ccccgaggga
     6301 gttcccatcg atgatccgaa ccgtcccgtg catccgcccc gtcctcggcg caaccctcgc
     6361 cgcgctgctg ggggtggcct cgctccacgc catggccgcg gacagcgcca tccctgcgtt
     6421 tcccggggcc atgggctggg ccgcgcagac gccgggcggg cgcggcggca ggatcatcaa
     6481 ggtcaccacg ctggcgccga cgggaccggg ctcgctgcgc gaggcgctgg acgccaaggg
     6541 accgcgcatc gtggtcttcg aagtcggtgg cgtgatcgac cttggcgtcg agacggtcaa
     6601 gatcagcgag cccttcctga ccatcgctgg acagacagcg ccgcaacccg ggatcaccct
     6661 gatccgcggc ggcatcgaca tcaccgcgca cgacgtcatc gtccagcaca tccgcgtgcg
     6721 tcccggcgac gccggcctgc cgaagatggg cggcaacgac ttcgacgcga tcagcacggt
     6781 cggcgcctac aacgtgatcg tcgaccactg ctcgctgacc tgggccagcg acgagaacct
     6841 gtcggcctcc ggcacccgct tcaagggcga caccccggag cagtggcgcg cgggcacgtc
     6901 gcaccggatc accttcagca acaacatcat cgccgagggc ctggcccatg cgaaccacat
     6961 caagggcgag cactcgaaag gctcgctgat ccacgacaac gcgcgcgaca tcctgatcgt
     7021 cggcaacctg tacgcgcaga acgtcgagcg caatccgttc ttcaagggcg gcacccaggg
     7081 ctgggtggtc aacaacctga tctacaaccc agggcagcgc gcgatccact acaacctgat
     7141 cgcggcggaa tgggccgggc atccgtacca ggccgggcga ctggcggtga tcggcaacgt
     7201 gatgcatgcc ggccccgaca cgccggccaa cgcggcgttc ttcatgttcg gcggttccgg
     7261 cgaactcgac ctgtacctgc atgacaacat cgcggtggat cgcatcggca accccatccc
     7321 gatgcagggc cgttacacca ccgcgccggt cgcgccgcgc atgctcaagg ccgcgccacc
     7381 aatgccgttc ggggtcgagc cgctgccggc caaccaggtg caggatgcgg tgatcgcgaa
     7441 cgtcggcgcg cgcccgtggg atcgcgacga catcgaccgc cgcatcctcg agaacaccat
     7501 cgaggggcgc ggccacatca tcgacagcca ggacgaagtg ggcggctacc cgaggcatgc
     7561 cgagaccagg caggccttcg tgccggcgga ctggaacctc gacacgatgg aaccgctgaa
     7621 gccgctcaag cagcgcgacc cgctgcgctg agtcgacgat ggaccgccag cgccgcgacc
     7681 tgctgaaggc ctcgctcgca ctcgggctgg cggccgccgc gccggccttc gccgcgccgc
     7741 gcctcgaacg cagcagcgca cgcacgcgca gtggccacgt gctcggcctg cgcaatggcg
     7801 acctgcatgt gttccgcggc atccgctacg gcgcggatac ttcaacgcat cgcttccagc
     7861 caccgcaacg tgaacttccg tggcgcggcg cacgtgaggc gctcgcgttc ggcgccagcg
     7921 caccgcaggg cggtggcgac ggcccgggca gcgaggactg cctgttcctc aacctcacca
     7981 cgcccgggct gcgggacggc aggaagcgac cgatcctgtt ctacatccac ggcggcggct
     8041 tcaacaatgg cagcggcagc gacccgttgt acgacggcag ccagctgtgc acgcatggcg
     8101 acgtggtggt ggtcaccgtc aaccatcgcc tgaacgcgtt cggctatctc tatctcggcg
     8161 agttcgacca gcgcttcgcc gagtccggca acgtcggcca gctcgatctc attgcagcat
     8221 tgcagtgggt gcgcgaacac gccgcggagt tcggtggcga tgccggcaac gtgaccgtat
     8281 tcggccagtc cggcggcggc gcgaagatcg ccacgctgat ggcgatgccg gccgcgcgcg
     8341 ggctgttcca tcgc
//
LOCUS       DAOOCX010000095         8300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_8108_length_8300_cov_9.062341, whole genome shotgun sequence.
ACCESSION   DAOOCX010000095 DAOOCX010000000
VERSION     DAOOCX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8108_length_8300_cov_9.062341"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="PK399_09110"
     CDS             <1..153
                     /locus_tag="PK399_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HPW13316.1"
                     /translation="FDQFVVLLRNTVSQMVYKHAISTVVPARNVRISATGVAEGEPSE
                     PEDGAE"
     gene            274..1617
                     /gene="hflX"
                     /locus_tag="PK399_09115"
     CDS             274..1617
                     /gene="hflX"
                     /locus_tag="PK399_09115"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPW13317.1"
                     /translation="MFERSRRGEHALLIQPHAGGPPGEGVLEEFGELARSAGATVAAV
                     VTAKIDRPNPATLIGSGKLEEVKAACDATGADLVLVNHALSPGQERNLEKLLERRVVD
                     RTGLILDIFSQRARSAEGKLQVELAQLKHVATRLVRGWTHLERQRGGSIGLRGPGETQ
                     LETDRRLLQKRVDMLQKRLEKVEVQHTQMRRARVRSELPRVALVGYTNAGKSTLFNAL
                     TGADAYAADQLFATLDPTVRRIELAGGGVVLADTVGFVRDLPHELVAAFRSTLSEARE
                     ADLLLHVIDANDPLREERMGQVDAVLREIGAGDIPQLQVFNKIDRIEGALPRIDQRGE
                     DRLAVWVSARDELGIDLLREALGERLGLQRVVGDILLPVDAGRLRARLHALDAVRGEQ
                     LEADGWRLQVDLPVADAARLAAQADGAPLRPLLPAIDTGLPMERDPAGLPVESDA"
     gene            1659..2732
                     /gene="hflK"
                     /locus_tag="PK399_09120"
     CDS             1659..2732
                     /gene="hflK"
                     /locus_tag="PK399_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HPW13318.1"
                     /translation="MAWNRPGGSSGKSTGGGGFGNLMDKLPDFGNGNPLRWIWIVLAL
                     WLAFNCFVLVNEQERGVVLRFGKFERVMQPGANFKLPWPLERVRMVNATKIETFNETV
                     PVLTRDENIVNVEINVQYRIGDAQAYLFGTRNADDVLKQAALSTVREQVGRADLDTVL
                     GARNALAVTARDQLQKSLATYGTGLNVTELNLPNARPPEEVKPAFDDVNSAQQDKDRV
                     TSEAIAYAAKVVPVARGEAARTRTVSEGYKTAAIARATGDATRFSLLVEQYKAAPDVT
                     RKRLWLDTVQQVLAENRTVVGGDGRQLIYVPMHGGKAPAGATTAPPATLAPELISPTV
                     EATAEPARSVRSGRAAGREEVVR"
     gene            2729..3592
                     /gene="hflC"
                     /locus_tag="PK399_09125"
     CDS             2729..3592
                     /gene="hflC"
                     /locus_tag="PK399_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPW13319.1"
                     /translation="MKFSSWMSAIVAALLLVASSMFVIPEGQTALVLKFGKVVRADLE
                     PGLHFKLPLVESLRRFDRRLQVLAAPAERYLTSERKDVSVDFFAIGRIEDVRAFYRAT
                     GGDEDAASARLAPIIKDALRNEINTRTLQQVVSGSRADVIGKQLENINLGARNMGVRI
                     VDLRIKQIDLPTDSRVIGDVYSRMRAQRQQVASRLRAEGEEQANQIRAKADRDRAVIL
                     AEAERDAQTLRGEGDAEATRLYAEAANRDPAFFAFQRSLEAYRKSFSSGDNVIVLERD
                     DPFLQYMRSDR"
     gene            3596..3781
                     /locus_tag="PK399_09130"
     CDS             3596..3781
                     /locus_tag="PK399_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 family protein"
                     /protein_id="HPW13320.1"
                     /translation="MADLWAALCLVAVLEGLVLFAAPGAWKRAVEQLQQMPDEHVRRI
                     GAIVLALGLVSLWLLRG"
     gene            complement(3812..5917)
                     /locus_tag="PK399_09135"
     CDS             complement(3812..5917)
                     /locus_tag="PK399_09135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPW13321.1"
                     /translation="MNVALKRLALLLVVSSLATFASAADTRKRPITAQDLWAVKRVGA
                     PALSPDGRQAVVSVQEWSIEKNKPTASLWLVDVASATTRRLTAGTGSDTAPAWSPDGS
                     RIAFTSKRLDDEAAALYLIPVAGGEAEKLVELPMGISVPAWLPDGKGIVFATQVIPDL
                     AGSFARADLAAMKKEAKRRKDAKMTAYVTEYRQYRFFDRNLTDNLAHRLLKVDVASKA
                     LTDLTPNYDRLFSPSGEVRFDISPDGHHVALALNSIPPPYAEQPNTDIVLLPTDGSGT
                     FTNLTTGNRYGDDYPRFAPDGRSVLYTRVASANAQGELRRLWRVSLAERKAMPLTTGI
                     DLSIDDVAFSADGRQLWLQAEDSGLVPVFAMDADGGAVRKIVGNGSNTELDSAAGQLV
                     FLGETTSRAAEVFALDASGKPRQLTRFNDALFAGLDLGKVESHTFPGAGGDSVQVWVT
                     YPPGFDPKKKYPLVQLLHGGPHTMVRDAFSPRWNSHVFASPGYIVASVNRHGSTGFGE
                     AYARSINGAWGDKPTEDVLKANDYLFATIPAIDRNNVAAAGASYGGYLATWLLGHTKA
                     FKALVNHAGVSDFMGQYGADTTTYGFTKEVLGGTPWDSPEAMQRNNPVAYAANFSTPM
                     LITHGEKDYRVPYGQGIALYGILQTMRVPSRLVVFPDENHWILSPQNSIYWNYEVQAW
                     LARYIGGKPMAKPEFKPAE"
     gene            6113..7405
                     /locus_tag="PK399_09140"
     CDS             6113..7405
                     /locus_tag="PK399_09140"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPW13322.1"
                     /translation="MGQSVVVLGAQWGDEGKGKIVDLLTQDIGAVVRFQGGHNAGHTL
                     VIAGKKTVLHLIPSGILRDDALCLIGNGVVLHPGALQKEIAELEGNGVEVRSRLKISP
                     ATPLIMPYHIALDQAREKAAGKSAIGTTGRGIGPAYEDKVARRGIRVADLHYPNELAE
                     KLRVALDYHNFVLTQYLKVDAVDYQQTLDEALAFGEYVEPMKADVAGILHDLRKAGKK
                     VLFEGAQGSLLDIDHGTYPYVTSSNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGG
                     GPFPTELDDAVGQGIRDRGQEYGATTGRPRRCGWMDIVALKRAVAINGISGLCITKLD
                     ILDGMEKLNICIAYEYRGKRTEYAPLDADGWDECTPVYLEFPGWSESTAGITEWDKLP
                     PAARAYLRALEELAGCPLAIVSTGPDRDANIVLRDPFA"
     gene            complement(7471..7926)
                     /locus_tag="PK399_09145"
     CDS             complement(7471..7926)
                     /locus_tag="PK399_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPW13323.1"
                     /translation="MAETIYDALRESHEIQRSPCRKLLRAKPGTQDRISLFKALHTEL
                     AAHAAAEERFLYAPVLMHDAGLDSSRHALHEHHEMDELVEDLHKADAEGGQWLAVARK
                     LSEKVHHHLREEEKKFFQVTGKILTDPQKVQAAKKYRKDYARMLKKLRG"
     gene            8087..>8300
                     /locus_tag="PK399_09150"
     CDS             8087..>8300
                     /locus_tag="PK399_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010368450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13324.1"
                     /translation="MRRTLVTAIALVLAGNGVAAPAPAAGDAPTASAAAGVTTQLPRT
                     ARPSHYAIEVTPHADRMTFDGKVRIDI"
BASE COUNT         1333 a         2758 c         2890 g         1319 t
ORIGIN      
        1 ttcgaccagt tcgtggtcct gctgcgcaat accgtcagcc agatggtcta caagcacgcg
       61 atctcgaccg tggtgcccgc gcgcaacgtg cgcatcagcg ccaccggcgt ggccgagggc
      121 gagcccagcg agcccgaaga cggggccgag tagcgtccag cgcccagggg caggcccccg
      181 tgcctgccct gagcgggggc ggatcgccca gggcaacgac agggggttgc cccgacggga
      241 ttcgcccgca tctagggttc gatgccaacc caattgttcg aacgctcccg ccggggcgag
      301 cacgccctgc tgatccagcc gcatgccgga ggcccgcccg gtgaaggcgt gctggaggaa
      361 ttcggcgaac tcgcgcgctc cgcaggcgcc accgtggccg cggtggtgac cgccaagatc
      421 gaccgtccga atcccgccac cctgatcggt agcggcaagc tggaggaagt gaaggccgcc
      481 tgcgacgcca ccggcgccga cctggtgctg gtcaaccacg cgctcagccc cggccaggag
      541 cgcaacctcg agaagttgct ggagcgtcgc gtcgtcgacc gcaccggcct gatcctcgac
      601 atcttctcgc agcgtgcccg cagtgccgaa ggcaagttgc aggtcgaact tgcgcagctc
      661 aagcacgtgg ccacccggct ggtgcgcggc tggacccacc tggagcgcca gcgcggcggt
      721 tcgatcggcc tgcgtggccc cggcgaaacc cagctggaaa ccgaccgccg cctgctgcag
      781 aagcgcgttg acatgctgca gaagcggctg gaaaaggtcg aggtccagca cacccagatg
      841 cggcgcgcgc gcgtccgcag cgaactgccg cgggtcgcgc tggtcggcta taccaatgcc
      901 ggcaagtcga cccttttcaa tgcgctcacc ggcgccgatg cctacgccgc ggaccagctg
      961 ttcgccacgc tggatcccac cgtgcgccgg atcgagctgg ccggcggtgg cgtggtcctg
     1021 gccgataccg tcggcttcgt ccgcgacctg ccgcatgaac tcgtcgccgc gttccgctcg
     1081 accctgagcg aggcgcgcga ggccgacctg ctgctgcacg tcatcgatgc caacgacccg
     1141 ctgcgcgagg agcgcatggg ccaggtcgat gccgtgctgc gcgagatcgg cgcgggcgat
     1201 atcccgcaat tgcaggtgtt caacaagatc gaccgcatcg agggcgcgct gccgcgcatc
     1261 gaccagcgcg gcgaggatcg actcgcggtg tgggtgtcgg cacgcgacga gcttggcatc
     1321 gacctgctgc gcgaggcgct gggcgaacgc cttggcctgc agcgcgtggt cggcgacatc
     1381 ctcctgccgg tcgatgccgg ccgcctgcgg gcgcgcctgc atgcgctgga tgccgtgcgc
     1441 ggtgagcagc tcgaggccga cggctggcgc ctgcaggtag acctgccggt cgcggacgcg
     1501 gcgcggctgg ccgcacaggc cgatggcgcg ccgttgcggc cgttgctgcc ggcgatcgac
     1561 accggacttc ccatggaacg cgacccggca ggcttgccgg tagaatcgga cgcatgacga
     1621 cgaaaagcag cggcccgcgc gggccaagag tgagctggat ggcgtggaac aggccgggcg
     1681 gttcttccgg caaatccacc ggtggcggtg gcttcggcaa cctgatggac aagctgccgg
     1741 acttcggcaa cggcaacccg ttgcgctgga tctggatcgt gctggcgctg tggctggcgt
     1801 tcaactgctt cgtcctggtc aacgagcagg agcgcggcgt ggtgctgcgc ttcggcaagt
     1861 tcgagcgggt gatgcagccg ggcgccaact tcaagctgcc gtggccgctg gagcgcgtgc
     1921 gcatggtcaa cgccaccaag atcgaaacct tcaacgaaac cgtcccggtg ctgacccgcg
     1981 acgagaacat cgtcaatgtc gagatcaacg tgcagtaccg catcggcgat gcgcaggcgt
     2041 acctgttcgg tacccgcaat gccgacgacg tgctcaagca ggccgcgctc agcacggtgc
     2101 gcgaacaggt cgggcgcgcc gacctggaca cggtgctcgg cgcgcgcaac gcgctggcgg
     2161 tgaccgcccg cgaccagttg cagaagtcgc tggcgaccta cgggacgggc ctgaacgtga
     2221 ccgagctcaa cctgcccaac gcgcgaccgc ccgaggaagt gaagccggcc ttcgatgacg
     2281 tcaacagcgc gcagcaggac aaggaccggg tcaccagcga ggcgatcgcg tatgccgcca
     2341 aggtggtgcc ggtggcgcgc ggtgaagccg ctcgcacgcg cacggtgtcc gagggctaca
     2401 agacggcggc gatcgcccgt gcgaccggcg acgcgacgcg cttcagcctg ctggtcgagc
     2461 agtacaaggc cgcgccggac gtgacccgca agcgcctgtg gctggatacc gtgcaacagg
     2521 tgctggccga gaatcgcact gtagttggtg gcgacggccg ccagctgatc tacgtgccga
     2581 tgcacggcgg caaggccccg gccggcgcga ccacggcgcc gcccgcgaca ttggcgccgg
     2641 aactgatttc gccgacggtg gaagccaccg cggagcccgc gcggagcgtg cgtagcggcc
     2701 gtgccgcggg tcgcgaggag gtcgtgcgat gaaattctcc agctggatgt ccgccatcgt
     2761 cgctgcgctg ctgctggtcg cgagctcgat gtttgtgatc cccgaaggcc agaccgcgct
     2821 ggtcctcaag ttcggcaagg tggtgcgcgc cgacctcgag ccgggcctgc acttcaagct
     2881 gccgctggtg gagagcctgc gtcgcttcga ccgccgcctg caggtgctgg ccgctccggc
     2941 cgagcgctac ctgacgtccg agcgcaagga cgtcagcgtc gacttcttcg cgatcgggcg
     3001 gatcgaggac gtgcgcgcgt tctaccgcgc caccggtggt gacgaggatg ctgcgtccgc
     3061 gcgcctggcc ccgatcatca aggacgcgct gcgcaacgag atcaacacgc gcaccttgca
     3121 gcaagtggtc tccggcagcc gtgccgacgt gatcggcaag cagctcgaga acatcaatct
     3181 cggcgccagg aacatgggcg tgcgcatcgt cgacctgcgc atcaagcaga tcgacctgcc
     3241 caccgacagc cgggtgatcg gcgacgtcta cagccgcatg cgcgcacaac gccagcaggt
     3301 cgccagccgc ttgcgcgccg agggcgagga gcaagccaac cagatccgtg cgaaggccga
     3361 ccgcgaccgc gcggtgatcc tggccgaggc cgagcgcgac gcgcagaccc tgcgcggcga
     3421 gggcgatgcc gaggcgaccc ggctatatgc cgaagcggcg aaccgcgatc ccgcgttctt
     3481 cgccttccag cgcagcctgg aggcctaccg caagtcgttc tcgagcggcg acaacgtgat
     3541 cgtgctggag cgcgacgacc cgttcctgca gtacatgcgc agcgaccgct gacccgtggc
     3601 cgacctttgg gcggcgttgt gcctggtcgc cgtgctggaa gggctggtcc tgttcgctgc
     3661 accgggtgcc tggaaacgcg ccgtcgagca attgcagcag atgccggacg aacacgtccg
     3721 gcgcatcggc gcgatcgtgc tggcgctcgg gctggtctcg ttgtggttgc tgcgcggctg
     3781 aaaaggcggt cagtgacgcg cggctggccg ttcactccgc tggcttgaat tccggtttcg
     3841 ccatcggctt gccgccgatg tagcgcgcca gccacgcctg cacttcgtag ttccagtaaa
     3901 tggaattctg cggcgacagg atccagtggt tctcgtccgg gaacaccacc agccgcgacg
     3961 gcacccgcat ggtctgcagg atgccgtaca gggcgatccc ttggccgtag ggcacgcggt
     4021 agtccttctc gccgtgggtg atcagcatcg gcgtggagaa attcgcggca taggccaccg
     4081 gattgttgcg ctgcatggcc tcggggctgt cccagggcgt gccaccgagc acttccttgg
     4141 tgaacccgta cgtcgtggta tccgcgccgt attggcccat gaagtcgctg acgccggcat
     4201 ggttgaccag ggccttgaac gccttggtgt ggcccagcag ccaggtcgcg agatagccgc
     4261 cgtaactcgc gccagccgct gccacgttgt tgcggtcgat ggccgggatc gtcgcgaaca
     4321 gatagtcgtt ggccttgagc acgtcctccg tcggcttgtc gccccaggcg ccgttgatgc
     4381 tgcgtgcgta ggcctcgccg aaaccggtgg agccatggcg gttgaccgag gcgacgatgt
     4441 agccggggct ggcgaatacg tggctgttcc agcgcgggct gaaggcatcg cgcaccatcg
     4501 tgtgcgggcc gccatgcagc agttgcacca gcgggtactt cttcttcgga tcgaagccgg
     4561 gcgggtaggt cacccacacc tgcacgctgt cgccaccggc gcccgggaag gtgtgcgatt
     4621 ccactttgcc caggtccagc ccggcgaaca gcgcatcgtt gaagcgggtg agctggcgcg
     4681 gcttgccgct ggcatccagg gcgaagactt ccgccgcgcg gctcgtggtc tcgcccagga
     4741 acaccagctg gcctgcggca ctgtccagct cggtattgct gccgttgccg acgatcttgc
     4801 gcaccgcgcc gccgtcggca tccatcgcga acaccggcac caggccgctg tcctcggcct
     4861 gcagccacag ctggcggcca tccgccgaga acgcgacgtc gtcgatgctc aggtcgatgc
     4921 ccgtcgtgag cggcatcgcc ttgcgctcgg caagcgacac ccgccacagc cgacgcaact
     4981 cgccttgcgc gttggcgctg gcgacgcggg tgtacagcac cgaacggccg tcgggggcga
     5041 agcgcggata gtcgtcgccg tagcgattgc cggtggtgag gttggtgaag gtgccgctgc
     5101 catcggtcgg cagcagcacg atgtcggtgt tgggttgctc ggcgtagggc ggcgggatgg
     5161 aattgagcgc cagcgcgacg tggtggccat ccggcgaaat gtcgaagcgc acctcgccgg
     5221 agggcgagaa caggcggtcg tagttcgggg tgaggtcggt caacgccttg ctggccacat
     5281 cgaccttcag cagccgatgg gcgaggttgt cggtcaggtt ccggtcgaag aagcggtact
     5341 ggcggtactc ggtgacgtag gccgtcatct tcgcgtcctt gcggcgcttg gcttccttct
     5401 tcatcgccgc gaggtcggcc ctggcgaaac tgccggccag gtcggggatc acctgggtgg
     5461 cgaagacgat gcccttgccg tccggcagcc aggcgggcac ggaaatcccc atcggcaatt
     5521 ccaccaactt ctcggcctcg ccaccggcga ccgggatcag gtagagcgcc gcagcttcgt
     5581 catcaaggcg cttgctggtg aaggcgatgc ggctgccatc cgggctccag gccggggcgg
     5641 tatcgctacc ggtaccggca gtgaggcgtc gcgtggtcgc actggcgaca tcgaccagcc
     5701 acaggctggc ggtcggcttg ttcttctcga tcgaccattc ctgcacgctg accaccgctt
     5761 gccggccatc gggcgagagc gccggcgcgc cgacccgctt gaccgcccac aggtcctgcg
     5821 cggtgatcgg gcgcttgcgg gtgtccgcgg cgctggcgaa tgtcgccagc gagctcacga
     5881 caagcaggag ggcaaggcgt ttcagcgcga cattcatggc ggatcccctg ttggcggcac
     5941 gcgcattcca tccccggagg cgtgccacgt tcaggttata cgcggatcgg ggcgcggcgt
     6001 acgggcggat ggcggcgacg gcggaaacgc gccacgacag cgggccgcga accccgtaga
     6061 atgtcgaaaa gccggggcag acctcggctt tttccatgtc tggagcagtg caatgggtca
     6121 gtcagtcgtc gttctcggtg cccagtgggg cgatgaaggc aagggcaaga tcgtcgacct
     6181 gctgacgcag gacatcggcg ccgtggtgcg tttccagggc gggcacaacg ccggccacac
     6241 gctggtgatc gccggcaaga agaccgtgct gcacctgatc ccgtccggca tcctgcgcga
     6301 cgacgcgctg tgcctgatcg gcaatggcgt ggtcctgcac ccgggtgccc tgcagaagga
     6361 aatcgccgag ctcgagggca acggcgtgga agtgcgcagc cgcctgaaga tcagcccggc
     6421 gacgccgctg atcatgccgt accacatcgc cctggaccag gcgcgcgaga aggccgcggg
     6481 caagtcggcg atcggcacca ccggtcgtgg catcggcccg gcctatgaag acaaggtggc
     6541 gcgtcgcggc atccgcgtcg ccgacctgca ctacccgaac gaattggccg agaagctgcg
     6601 cgtggcgctc gactaccaca acttcgtgct gacccagtac ctgaaggtcg atgccgtcga
     6661 ctaccagcag accctggacg aggcgctggc ttttggcgag tacgtcgagc cgatgaaggc
     6721 cgacgtggcc ggcatcctcc atgacctgcg caaggccggg aagaaggtgc tgttcgaggg
     6781 cgcgcagggt tcgctgctcg acatcgacca tggcacctat ccgtacgtga cgtcgtcgaa
     6841 taccaccgtc ggcggcgcgc tggccggcac cggcgtgggc gcggacgcga tcgactacgt
     6901 gctcggcatc gccaaggcgt atgcgacgcg cgtcggcggc ggcccgttcc cgaccgagct
     6961 ggacgacgcc gtcggccagg gcatccgcga ccgcggccag gaatacggcg cgaccaccgg
     7021 tcgtccacgt cgctgcggct ggatggacat cgtggcgctc aagcgcgcgg tggcgatcaa
     7081 cggcatcagc ggcctgtgca tcaccaagct cgacatcctc gacgggatgg agaagctcaa
     7141 catctgcatc gcctacgagt accgcggcaa gcgcaccgaa tacgcgccgc tggatgcgga
     7201 tggctgggac gagtgcacgc cggtgtacct ggagttcccg ggctggagcg agtccaccgc
     7261 cggcatcacc gagtgggaca agttgccgcc ggcggcgcgc gcctacctgc gtgcactgga
     7321 ggaactggcg ggttgcccat tggcgatcgt cagcactggc ccggaccgcg acgccaacat
     7381 cgtcctgcgc gatccgttcg cctgagccac gtgcagctcg gcacgaaaac gccccgcatg
     7441 acggggcgtt ttcgtttgtg gtgacggacg tcagccgcgc agtttcttga gcatccgcgc
     7501 gtagtccttg cggtatttct tcgccgcctg cactttctgc ggatcggtca ggatcttgcc
     7561 cgtgacctgg aagaacttct tttcttcctc gcgcaggtgg tggtgcacct tttccgagag
     7621 cttgcgcgcc accgccagcc actgcccgcc ttcggcgtcg gccttgtgca ggtcctcgac
     7681 cagctcatcc atctcgtggt gttcgtgcag cgcgtggcgc gacgaatcca ggccggcgtc
     7741 gtgcatcagc accggcgcgt agaggaagcg ttcttccgcg gcggcatgcg cggccaattc
     7801 cgtatgcagc gccttgaaca gcgagatgcg gtcctgggta ccgggcttgg cgcgcagcaa
     7861 cttgcgacag ggcgagcgct ggatttcgtg gctctcgcgc agggcgtcgt agatggtctc
     7921 ggccatgtcg ggcctccgct gaacaggtgc gaagattcga cgcgatggcg cgatggcgat
     7981 gtgaatgcgc accggcatga cttccggcgc tggccggggt gcgggggagg ggcggatgat
     8041 cgcgggcttc cgggcaggac ctgcctgctc tccaacaagg atttccatgc gccgcaccct
     8101 ggttaccgcg atcgcactgg tgcttgccgg caacggtgtc gccgcccccg cacctgccgc
     8161 cggcgatgcg cctaccgctt ccgccgccgc cggcgtcacc acccagttgc cgcgcacggc
     8221 ccggccgagc cactacgcga tcgaagtcac cccgcatgcg gacaggatga cgttcgacgg
     8281 caaggtccgg atcgacatcg
//
LOCUS       DAOOCX010000096         8196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_8250_length_8196_cov_8.021987, whole genome shotgun sequence.
ACCESSION   DAOOCX010000096 DAOOCX010000000
VERSION     DAOOCX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8250_length_8196_cov_8.021987"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PK399_09155"
     CDS             complement(<1..1376)
                     /locus_tag="PK399_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HPW13325.1"
                     /translation="MAFLIVLAALCFLMFVAYRGYSVILFAPVAALGAVLLTDPHLVA
                     PMFTGLFMDKMVGFLKLYFPVFLLGALFGKVIELSGFSKSIVAATIRVVGAQRAMLAI
                     VAVCALLTYGGVSLFVVVFAVYPFAAELFRQSDIPKRLIPGTIALGAFTFTMDALPGT
                     PQIQNIIPTTFFGTDTWAAPVLGTLGGVFILIVGMVYLDWRRRVALRAGEGYGDAATL
                     VNEPVAFAGGKLANPMIAILPLLVVGIVNKLLTLWIPGVYGESHSFDPAVIGNAVPVV
                     QEVSKLAAIWAVEGALLAGIACVLLFAWKPVTSSFAEGSKSAIGGALLAGMNTASEYG
                     FGAVIAALPGFLVVANALGSIPNPLVNEAVTVTALAGITGSASGGMSIALAAMAETFI
                     ANANAAGIPMEVLHRVAAMASGGMDTLPHNGAVITLLAVTGLTHRQAYKDIFAVTLIK
                     TMAVFVVIG"
     gene            1536..3785
                     /locus_tag="PK399_09160"
     CDS             1536..3785
                     /locus_tag="PK399_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW13326.1"
                     /translation="MKRKQLSRAIHGVLAMSLLAGTGTVMAQDAAPAADGPKTTTLEG
                     IKVTARKREETLQEVPVAVTAFTADSLDRLNVKDLADLDGQVPNLTIYAARGSSSTLT
                     AYIRGVGQSDPLWGVDPGVGIYMDDVYIARPQGALLDVFDVDRIEVLRGPQGTLYGKN
                     TIGGAIKYISKGLRSDFNGYGSVTLGNYGQRDIKAALGGGFGGSEYLRGRISVADLHR
                     DGFGENLVTGAQVSDKKVRAVRANLGAYVTDSFDLQFAFDHVDDGSGVRGAQMLRANP
                     YDALLTPTTGPFLPLADRYDVRNGMPNVNDTTLSGASVVANFRMNEDWTFKYVLAKRE
                     SDTETNIDFDTTPDKIADVKAFYSDEQVTHEFQANYDAGGRSRGVIGIYKFDGEAGGQ
                     VLNNFFNLSFGDTQGTVYTESIAAYADWTFDVSDKFSIDAGLRWTNEDKHAVVRNIGY
                     TDGTFTKPSGVVAAAFDKTINFKNLSPKISLDYQVTPDIMVYGLASRGFKSGGYNIRA
                     QATAVPRSAEPFQDEQVDSFEVGTKMGLLDQRLFLNLSAFHNKYKDIQLSIFTEYTTP
                     QNTKAFFGDFTNAGEGTVNGFEVEYQWMATDNFNISGNLAWLDAKFDTYMFKNVNIAD
                     QQEFTNAPDFSGALNAEYRIDLDGGGSLSARVGYTYQSRVVATTEVVKDPVTLATVQP
                     ITQDGYGLVGAGIIWKANDAWTVSLQGSNLTDKEYRTTGYNLYAALGVHTGFYGPPRQ
                     YSLTVKYDF"
     gene            3905..7222
                     /locus_tag="PK399_09165"
     CDS             3905..7222
                     /locus_tag="PK399_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HPW13327.1"
                     /translation="MLSVAVVVAAAVLWLGLLFGTALYAERRPGVLAGHWRHVYALSL
                     AVYCTSWTFFGTVTQAARYDWPLPPTFVGTLLLYALGAGLMLQLVKLARASNATSIAD
                     LIATRLGKDAWLAAGVTLIAVLGLIPYVALQLKAVSMSFALLTDSQAGADQPPWRDSS
                     LYVALVMGLFAMLFGTRRASAAEHNRGLVLAMAFESLFKLVAMLALGLFVWLGLGLPS
                     PPSLPEAAASTDNGGFIPLVLLGVLAMFTLPHQFHVGVVECRDERHVLTARWLFPLYL
                     VLIALPVLPLARAGDALLAGQGVPSDLYVLALPLSQSHTGLALFAFLGGLSAATGMVV
                     VSTLTLSLMIGNHWLAPGLLRGAWARGDAGDLRGNVLLLRRLGIAAIMLLAWAYSRSI
                     AGNAALADVGALSFSALATLAPALGFAVWRTQTPPRAALVGMVAAFLTWAWCLPVPLL
                     LRDDAPAWLQGGPFGIAWLAPEGFFGLTGWSPLGRAVAASLFVGTFTTLLLSAWHRPV
                     ASLGRRRLDARSLDNVARRFLPATQVEHLLARGRDAETEIERALSSVLGSASARVLLD
                     AARRDATDAGRPGADLDTVAAIVGEASQDLRFNQRVLEAALENMSQGISVVDAELRLV
                     AWNRRYAELFGYPPGMLRVGMPIAEASQWALRALGDVDDDDAALQRRLAHMRAGTAHL
                     SERVFPDGSIVEIRGNPMPGGGFVATFTDVTAFRQAETGLKRINETLEQRVGERTSML
                     ETAKREAERANDAKSRFLAAIGHDLLQPLHAAHLFADALQQRGDGEQRELARQIGGAL
                     DSTTDLLGTLLDMSRLEAGGLVPELRDFPLAEVLDPLVAQFDVLARERGLRLRHVPTR
                     AWVRSDPQLLRRVLQNFLANAVRYTASGSVLLGVRRDGDALRIEVHDTGPGIEPVRFD
                     AIFEEFRRGEDAPGQGLGLGLAIARRIAVLLGSDVDLHSRPGRGSRFGIEVPRVAAMQ
                     PHRNAKRGLAGLRVLLVDNEPQALEALASVLRGWGCEVRAVADGDAARVELSRGTADL
                     WVFDYHLDHGDDGVSLRQRLSLEFGNVPCLILSADQTGAVRSAAQEAGLPLLVKPLRP
                     LALKSVLDRMLAARGVA"
     gene            7344..8078
                     /locus_tag="PK399_09170"
     CDS             7344..8078
                     /locus_tag="PK399_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HPW13328.1"
                     /translation="MRNVLIYDAPTRVFHWLFAGFFAAAFAIANLVDDESARFSLHML
                     AGLCMVFVLLLRIVWSLVGTRHARASDLVLNPARLFGYFRDMVSGRGKEWAGHNPASS
                     WAAVTMASLALGLGITGTLMATGGESGSVKELHELLANGFLAVVLLHLGGVVVHALRH
                     RDRLPSSMLTGRKQLRADGYAPIRPLPLAGLAFLALTVLFIGHLARTYDAQARTVDVF
                     GITLQLGENEDSHASGDTEEDEHEDR"
BASE COUNT         1293 a         2820 c         2752 g         1331 t
ORIGIN      
        1 ccgatcacca cgaataccgc catcgtcttg atgagcgtga ccgcgaagat gtccttgtag
       61 gcctgccgat gggtcagtcc cgtcaccgcg agcagggtga tcaccgcgcc attgtgcggc
      121 aacgtgtcca tgccgccgga ggccatcgcg gccacgcggt gcaacacctc catcgggatg
      181 ccggcggcgt tcgcgttggc gatgaaggtc tcggccatcg ctgccagcgc gatgctcatg
      241 ccgcccgatg cgctgccggt gatgccggcc aatgcggtca cggtgaccgc ttcgttgacc
      301 agcggattcg ggatcgagcc cagtgcattg gcgaccacga ggaagccggg cagggcggcg
      361 atgaccgcgc cgaagccgta ttccgacgcg gtgttcatgc cggccagcaa cgcgccgccg
      421 atcgccgact tgctgccctc ggcgaagctc gaggtcaccg gcttccatgc gaacagcagc
      481 acgcaggcga tgccggccag caaggcgcct tccaccgccc agatcgcggc cagcttcgat
      541 acttcctgta cgaccggcac ggcgttgccg atcaccgccg gatcgaagct gtggctctcg
      601 ccatagacgc ctgggatcca cagcgtcagc agcttgttga cgatgccgac caccagcaac
      661 ggcaggatcg cgatcatcgg attggccagc ttgccgccgg cgaacgcgac gggttcgttc
      721 accagcgtcg cagcatcgcc atagccttcg ccggccctga gggcgacgcg gcggcgccag
      781 tccaggtaga ccatgccgac gatcaggatg aacacgccgc ccaacgtgcc cagcaccggc
      841 gcagcccagg tgtcggtgcc gaagaaggtg gtcgggatga tgttctggat ctgcggcgtg
      901 cccggcagcg cgtccatggt gaaggtgaac gcgcccagcg cgatggtgcc cgggatcagc
      961 cgcttgggga tgtcggactg gcggaacaat tccgcggcga aggggtagac cgcgaacacc
     1021 accacgaaca gcgagacgcc gccgtaggtc agcaaggcgc agaccgcgac gatcgccagc
     1081 atcgcccgtt gtgccccgac cacgcggatg gtcgcggcca cgatcgactt cgagaaaccc
     1141 gacagctcga tgaccttgcc gaacaacgcg ccgagcagga acaccgggaa gtacaacttg
     1201 aggaacccga ccatcttgtc catgaacagg ccggtgaaca tcggcgcgac caggtgcgga
     1261 tcggtcagca gcaccgcgcc cagcgccgcc accggcgcga acaggatcac gctgtagccg
     1321 cggtaggcca cgaacatcag gaagcacagc gcggccaaca cgatcaggaa tgccattgcg
     1381 cccccgtggg catcgatcag gggtgcgagt ctcccggtcg tggggatcgc ggcccattgt
     1441 ccgaacgtcc aatgccgtta atcgtcctta acacctaccg tgcaccccat cggcggcgtg
     1501 ggcccgccca cgtgcaccta ccctggaggg gacagatgaa gcgcaaacaa ttgagcaggg
     1561 cgatccatgg cgtgctggcg atgtcgttgc tggccggcac cggcaccgtg atggcacagg
     1621 acgccgcgcc ggcggcggac ggcccgaaga ccacgacact cgaaggcatc aaggtcaccg
     1681 cccgcaagcg cgaggagacc ctgcaggaag tgccggtcgc ggtcaccgcg ttcaccgcgg
     1741 actcgctgga caggctcaac gtcaaggacc tggccgacct cgacgggcag gtgccgaacc
     1801 tgacgatcta cgcagcgcgt ggctccagca gcacgctgac cgcctacatc cgcggcgtcg
     1861 gccagtccga tccgctgtgg ggcgtggatc cgggcgtggg catctacatg gacgacgtct
     1921 acatcgcccg tcctcagggc gccttgctcg acgtgttcga cgtcgatcgc atcgaagtgc
     1981 tgcgcggccc gcagggcacg ctgtacggca agaacaccat cggcggcgcg atcaagtaca
     2041 tctccaaggg cctgcgctcc gatttcaacg gctacggctc ggtgaccctg ggcaactacg
     2101 gccagcgcga catcaaggcc gcgctcggtg gcggcttcgg cggcagcgaa tacctgcgcg
     2161 gccgcatctc ggtggccgac ctgcaccgcg acggcttcgg cgagaacctg gtcaccggcg
     2221 cgcaggtcag cgacaagaag gtccgcgcgg tccgcgccaa cctgggcgcc tacgtcaccg
     2281 acagcttcga cctgcagttc gcgttcgacc atgtcgatga cgggtccggc gtgcgcggcg
     2341 cgcagatgct ccgcgccaat ccgtacgacg cgctgctgac cccgaccacc ggcccgttcc
     2401 tgccgctggc ggaccgctac gacgtacgca acggcatgcc caacgtcaac gacaccacct
     2461 tgagcggtgc gtccgtggtc gccaacttcc gcatgaacga agactggacc ttcaagtacg
     2521 tgctggccaa gcgcgagtcg gataccgaga ccaacatcga tttcgacacc accccggaca
     2581 agatcgccga cgtcaaggcg ttctacagcg acgagcaggt cacccacgaa ttccaggcca
     2641 actacgatgc cggcggtcgt tcgcgcggcg tgatcggcat ctacaagttc gacggcgagg
     2701 cgggtggcca ggtcctcaac aatttcttca acctgagctt cggcgacacc cagggcacgg
     2761 tctacaccga gagcatcgcc gcgtacgccg actggacctt cgacgtcagc gacaagttca
     2821 gcatcgacgc gggcctgcgc tggaccaacg aggacaagca cgcggtggtc aggaacatcg
     2881 gctacaccga tggcaccttc accaagccca gcggcgtggt ggcggctgcg ttcgacaaga
     2941 ccatcaactt caagaacctg tcgccgaaga tctcgctgga ctaccaggtc accccggaca
     3001 tcatggtcta tggcctggcc tcgcgcggct tcaagtcggg cggctacaac atccgcgcgc
     3061 aggccaccgc ggtcccgcgt tcggccgagc cgttccagga cgagcaggtc gacagcttcg
     3121 aggtgggcac caagatgggc ctgctcgacc agcgcctgtt cctgaacctg tcggcgttcc
     3181 acaacaagta caaggacatc cagctctcga tcttcaccga gtacaccacg ccgcagaaca
     3241 ccaaggcgtt cttcggcgac ttcaccaacg ccggcgaggg cacggtcaac gggttcgaag
     3301 tcgaatacca gtggatggcc accgacaact tcaacatctc cggcaacctg gcctggctgg
     3361 atgcgaagtt cgatacctac atgttcaaga acgtcaacat cgccgaccag caggaattca
     3421 ccaacgcccc ggatttctcc ggcgcgctga acgccgaata ccgcatcgac ctggacggcg
     3481 gcggcagcct gtcggcgcgc gtgggttaca cctaccagag tcgcgtggtg gccacgactg
     3541 aagtggtcaa ggacccggtg accctggcca ccgtgcagcc gatcacccag gacggctatg
     3601 gcctggtggg cgccggcatc atctggaagg ccaacgatgc gtggaccgtc tccctgcagg
     3661 gcagcaacct gaccgacaag gaataccgca ccaccggcta caacctgtac gccgcgctgg
     3721 gcgtgcacac cggcttctac ggcccgccgc gccagtacag cctgaccgtg aagtacgact
     3781 tctgatcgaa gcctgccacg cgttgcaact ccgacggcgg gccttggccc gccgtcgcgt
     3841 tatcctcgcc gcgcatgcga tgccttgacc attcgccacg atgcccatgc ctgacggtgg
     3901 cgcgatgctg agcgtcgcgg tcgtcgtcgc ggctgcggtg ctgtggctgg gcctgctgtt
     3961 cggcaccgcc ctgtatgccg agcgtcgccc gggtgtcctc gctggccact ggcgccacgt
     4021 gtacgccttg tcgctggcgg tctactgcac gtcatggacg ttcttcggca ccgtcaccca
     4081 ggccgcgcgc tacgactggc cgctgccgcc caccttcgtc ggcaccctgc tgctgtacgc
     4141 gctcggcgcg gggctgatgc tgcaactggt gaagctggcg cgcgccagca atgcgacctc
     4201 gatcgccgac ctgatcgcca cccgcctggg caaggacgca tggctcgccg cgggcgtgac
     4261 gttgatcgcg gtgctcggcc tgattccgta cgtcgccctg cagctgaagg cggtgtcgat
     4321 gagtttcgcc ctgctgaccg actcgcaggc cggggccgac cagccgccgt ggcgcgacag
     4381 ttcgctgtac gtcgcgttgg tgatgggcct gttcgcaatg ctgttcggca cccgtcgcgc
     4441 cagcgcggcc gagcacaacc gcgggctggt gctggcaatg gcgttcgagt cgctgttcaa
     4501 gctggtggcc atgctggcgc tgggcctgtt cgtctggctg ggcctgggat tgccatcgcc
     4561 gccgtcattg ccggaagccg cggcatcgac cgacaacggc ggcttcatcc cgctggtgtt
     4621 gctgggcgtg ctggcgatgt tcaccctgcc gcaccagttc catgtcggcg tggtcgaatg
     4681 ccgcgacgag cgccacgtcc tgaccgcccg ctggttgttc ccgctgtacc tcgtcctgat
     4741 cgcgttgccg gtgctgccgc tggcgcgcgc cggcgatgcg ctgctggccg gacagggcgt
     4801 gccctccgac ctgtacgtgc tggcgctgcc gttgtcgcaa tcgcacaccg ggctggcgct
     4861 gttcgcgttc ctcggaggac tcagcgcggc caccggcatg gtggtggtca gcacgctcac
     4921 cttgagtttg atgatcggca accactggct ggcccccggc ctgttgcgcg gcgcgtgggc
     4981 gcgtggcgac gccggcgacc tgcgcggcaa cgtgctgttg ctgcgccggc tcggcatcgc
     5041 cgcgatcatg ctgctggcct gggcctacag tcgctcgatc gccggtaatg cggcgctggc
     5101 cgatgtcggc gcgctgtcgt tctcggcgtt ggcgacgctg gccccggcgc tgggcttcgc
     5161 ggtgtggcgc acgcagaccc cgccacgcgc ggccctggtt ggtatggtcg ccgcgttcct
     5221 gacctgggcc tggtgcctgc cggtgccgct gctcctgcgc gacgacgcac cggcgtggtt
     5281 gcaggggggg ccgttcggca tcgcctggct ggcacccgag ggcttcttcg gcctcaccgg
     5341 ttggagcccg ctgggtcgcg ccgtcgccgc cagcctgttc gtcggcacgt tcaccacgtt
     5401 gctgctgtcg gcctggcatc ggccggtggc cagcctgggc cgacgccggc tggatgcgcg
     5461 cagcctggac aacgtcgcgc gccgcttcct gccggcgacg caggtcgagc acctgctggc
     5521 gcgcggtcgc gacgccgaaa ccgagatcga acgagcgttg tcgtcggtgc tcggttcggc
     5581 gtcggcgcgc gtgttgctgg atgcggcccg tcgcgatgcc accgatgccg gtcgacccgg
     5641 tgccgacctc gacaccgtcg ccgcgatcgt cggcgaggcc agccaggacc tgcgcttcaa
     5701 ccagcgcgtg ctggaagcgg cgctggagaa catgagccaa ggcatcagcg tggtcgatgc
     5761 cgagctgcgg ctggtcgcat ggaatcgccg ctacgccgaa ctgttcggct acccgcccgg
     5821 catgctgcgg gtgggcatgc cgatcgccga ggcatcgcaa tgggcgctgc gtgcgctcgg
     5881 cgacgtggac gatgacgatg ccgccctgca gcgccggctg gcgcatatgc gtgccggcac
     5941 cgcgcacctg tcggaacgcg tgttcccgga cgggagcatc gtggaaatcc gcggcaaccc
     6001 gatgccgggc gggggcttcg tcgccacctt caccgacgtc accgcgttcc gccaggccga
     6061 gaccggcctc aagcgcatca acgaaaccct cgagcagcgt gtcggcgaac gcacgtcgat
     6121 gctcgagacc gccaagcgcg aggccgagcg cgccaacgac gccaagagcc gcttcctcgc
     6181 cgcgatcggc cacgacctgc tgcagccgct gcatgccgcg cacctgttcg cggacgcctt
     6241 gcagcagcgc ggcgatggcg agcagcgcga gctcgcccgg cagatcggcg gcgcgctgga
     6301 ttccaccacc gacctgctgg gcacgctgct ggacatgtcg cggctggaag cgggtggact
     6361 ggtgccggag ctgcgggatt ttccgctggc cgaggtgctg gatccgctgg tcgcgcagtt
     6421 cgacgtgctc gcccgcgagc gcggcctgcg cctgcgccac gtgcccaccc gcgcgtgggt
     6481 tcgcagcgac ccgcaactgc tgcgccgcgt cctgcagaat ttcctggcca atgcggtccg
     6541 ctacaccgcg agcggcagcg tgctgctggg tgtgcgccgc gatggcgatg cgctgcgcat
     6601 cgaggtccac gacaccggcc ccgggatcga gccggtgcgc ttcgacgcga tcttcgagga
     6661 gttccgccgc ggcgaggatg caccggggca ggggcttggc ctgggcctgg ccatcgcccg
     6721 ccgcatcgcg gtcctgctcg gttccgatgt cgacctgcac agcaggcccg ggcgcggcag
     6781 ccgcttcggc atcgaggtgc cgcgggtggc agccatgcag ccccatcgca atgcgaagcg
     6841 cggcctggcg ggcctgcgcg tgctgctggt ggacaacgaa ccgcaggcgc tggaggcgtt
     6901 ggccagcgtg ctgcgaggct ggggatgcga ggtgcgtgcc gtggccgatg gcgacgccgc
     6961 gcgcgtcgag ctgtcgcgcg gcaccgccga cctctgggtc ttcgactacc acctcgacca
     7021 tggcgatgac ggcgtgtccc tgcgccagcg cctgtccctc gagttcggca acgtgccctg
     7081 cctgatcctg agtgccgacc agacgggggc cgtgcgcagc gccgcgcagg aagccggctt
     7141 gcccctgctg gtgaagccat tgcgtccgtt ggcgctgaag tcggtgctgg accgaatgct
     7201 ggcggcgcgg ggtgtggcgt agctcgccat gggtgcccgt tcggccgcaa attgttgatt
     7261 tgattggcgt tagggcattc gccttatggt cgccggcaga ggcggcgccc acgatcggag
     7321 cctcccgacc gagagagcct gccatgcgca acgtgctgat ctacgatgcc cccacccgag
     7381 tgttccactg gttgttcgcg gggtttttcg ccgcggcgtt cgccatcgcg aacctggtcg
     7441 atgacgagtc ggcgcgattc tcgctgcaca tgctggcggg gctgtgcatg gtgttcgtgc
     7501 tgctgctgcg gatcgtctgg tcgctggtcg ggacacgcca tgcccgcgca agcgatctcg
     7561 tcctgaaccc cgcgcgattg ttcggctatt tcagggacat ggtctcgggt cgtggcaagg
     7621 aatgggccgg ccataacccg gcctccagct gggccgcggt gacgatggcc agcctggcgc
     7681 tgggccttgg catcaccggc accctgatgg cgaccggcgg cgaatccggg tcggtcaagg
     7741 aactgcatga gttgctggcc aatggcttcc tggccgtggt cctgctccac ctgggcggcg
     7801 tggtggtgca tgcactgcgg catcgcgacc gcctgccatc gagcatgctc accggcagga
     7861 agcagctgcg cgccgacgga tacgcgccga tccgtccgct gccgctcgcc ggcctggcct
     7921 tccttgcctt gaccgtcctg ttcatcggcc atctcgcgcg gacctatgat gcgcaggcgc
     7981 gcaccgtcga cgtattcggc atcaccctgc agttgggcga aaacgaggat agccatgcat
     8041 ccggcgacac cgaagaagac gagcacgaag atcgatagtc ctgcccagcg cgccggcacc
     8101 gagccgccgg tgcgcggcct gcagggcgcc gaacgcgcgg tgatcgccgc ggtgctggcc
     8161 ctgatcgcac tgctagccgg cctggacctg ctggtc
//
LOCUS       DAOOCX010000097         8012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_8502_length_8012_cov_8.060199, whole genome shotgun sequence.
ACCESSION   DAOOCX010000097 DAOOCX010000000
VERSION     DAOOCX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8502_length_8012_cov_8.060199"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="PK399_09175"
     CDS             complement(<1..158)
                     /locus_tag="PK399_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPW13329.1"
                     /translation="MSNRDTTRALWQIHLCVLLWGFTAILGKLITLPALPLVWWRMLI
                     VVAALALLP"
     gene            190..564
                     /locus_tag="PK399_09180"
     CDS             190..564
                     /locus_tag="PK399_09180"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HPW13330.1"
                     /translation="MGREYLDYLHDLFSAFAPVTTRAMFGGHGVYRDGVIIAIVIDEL
                     LYLKVDDQTRAAFEAEGCAPFVYEAQGKRVPMSYWTVPDEALDSPQEFRPWAQRAWEA
                     ALRKPKAKPKKRKASKRKMGSE"
     gene            complement(614..1996)
                     /locus_tag="PK399_09185"
     CDS             complement(614..1996)
                     /locus_tag="PK399_09185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW13331.1"
                     /translation="MPSGTSPPVGGGGIVCGMDTHRPRYARLQQRIAGLFNLRRGELA
                     PVLVAGLFFFCILTALQMLRPARDALGMERGIESIRWLFIGTAVVTLLVNPLFGWLVS
                     RLRRLQFISATYGFFALSLAGFWGLLVFAPGAVGARSGQVFYVWFSVFNLFVTMVFWA
                     LLADRFSSEQGKRLFALIAVGGTLGAIFGPWLASQLSELLGTPALLLVAAGFLLLGIA
                     LAWWLVRIAGDRATTPTQVDDVRDGEQRIGGSAWAGLRAVFSSPYLLGIAGYVLAMTI
                     VATFVYFTRLQMVAAAESDLDARTGLLASIDMWTQVAVLLLQLTLTGHVIRRFGLAFA
                     LALLPIANALGFIGLAIFGSFAALVLLEALNKAVQRGLTRPAREALFTVISREDKYKA
                     KAVIDTFVYRGGDVAGAQVEGLLGRLGMALGGLVTVVVPLALAWAMLGLWLGRAQGRK
                     ATSEQKMGSE"
     gene            complement(2014..2859)
                     /locus_tag="PK399_09190"
     CDS             complement(2014..2859)
                     /locus_tag="PK399_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPW13332.1"
                     /translation="MSLFPDTDDWTSQRVGAVDGIRCGIGGWTYAPWRDNFYPKGLVQ
                     RRELEYASRHLSCIEINGTYYGAQKPATYAKWRDETPDGFVFSVKAPMRIMQSKALAR
                     TGPQVEDFIGGIGTLGDRLGPLLWQFDAGLTLQRDAFEAFCDLLPRTVEGRALRHVLD
                     VRDAAFIDADFIALARKHGFATVFTDSPEHPSFADVTAEFVYARLMRSRSDIATGYPL
                     GELEGWAARARLWASGGEPGDLPKITDAVPTHEPRDVFIQFISAAKERNPAAAMALTA
                     LLARG"
     gene            complement(2856..3293)
                     /locus_tag="PK399_09195"
     CDS             complement(2856..3293)
                     /locus_tag="PK399_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW13333.1"
                     /translation="MSQPANTRASIIPCLRYRNALGMIEWLCEAFGFQQHAVYAEGDT
                     VHHAQLVYGNGMVMLGSTSNAGEWGKAIVQPDEIGGRETQSACVIVADADAHYARAVA
                     AGATIVIDIADQPYGGRGYACKDPEGHYWWFGSYDPWAEQSPS"
     gene            3560..5059
                     /locus_tag="PK399_09200"
     CDS             3560..5059
                     /locus_tag="PK399_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPW13334.1"
                     /translation="MARFDAFVAAVQKQFDVPGVAVAIVKDGQVVLERGYGVRKLGEP
                     AKVDAHTMFAIASNTKAFTAAALNMLQDEGKLKTTDRVIDHLPWFRMSDPYVTREMRI
                     RDLLAHRSGLSLGAGDLLYWPTTSYTTREVAERLKDVPLTGGFREQYAYDNILFGVAQ
                     LVIEEASGMPYKHFLDRRIFQPLGMAETRYNSDDLKPGDNVATGHAKFDFKDLRPVGV
                     TSWRNVSGAGGLYSSVHDLSKWMNVQLAGGVYAGSGDAAKRLFSDARQREMWTVLTPI
                     PVGKSSVPGLAAMTPNFMGYGQGWNLSDYAGERLVWHTGGWPGQVSRLTLVPGEKLGV
                     VVLTSGEVGQAFHAITYEALDMMLGRSGNDWLQAYGESFARAQGNADEDWKKHVAARD
                     ATSKPSLALANYAGTYRDPWYGDVLIRQGAKGLEMQFTKTAELLGDIEHWQHDSFIVR
                     WRDRGLNADAFVNFSLDPDGRIRELRMEPISPLTDFSFDFQDLRLAPVK"
     gene            complement(5155..5508)
                     /locus_tag="PK399_09205"
                     /pseudo
     CDS             complement(5155..5508)
                     /locus_tag="PK399_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(5854..6747)
                     /locus_tag="PK399_09210"
     CDS             complement(5854..6747)
                     /locus_tag="PK399_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13335.1"
                     /translation="MSTTRPTSLSVRSALGEGLRGAMQWRLWLLWIGASLACALIAAL
                     PAWNWLADGLNHSVQAKAIASGQAPIPLLDLLMSRTAPLGVLGESTTVAAVLMLLLSP
                     LLAGATLAAIRSRTRPGFGDLLRGALSEYGPLLRMLLWSAIPLGLALMVMSVIIGANE
                     SAHEHAIEASAVTTGRNIAFVVGGVLFVLAHASLEAGRGWLATDGRLRSALKAWWRGL
                     KLLARRPIAVLLAWLVTTALGLALALLFAWLRQFAGGAGIGGFLLAMLLGCGIAAGLA
                     WGRIARLYAMSALAADQHARR"
     gene            complement(6758..>8012)
                     /locus_tag="PK399_09215"
     CDS             complement(6758..>8012)
                     /locus_tag="PK399_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HPW13336.1"
                     /translation="VKTWNGPNGPVQVSVLYTPDYASNAEPVLQATIDSLDYFSKTLG
                     GYPYGTATAVVPPYGADEAGGMEYPTFFTADSTSQDEPGTLGRFALDFVTVHEFGHGY
                     FYGILASNEFEEPMLDEGMNEYWDQRMMSGRKQDLILTTPFLRALGIGTKLTPFDMER
                     LGASLADPADALGDNSWSRLSSGSYGTVYSRTATVMRQIEALVGTPAMERAMKLYYER
                     WKFRHPSLADLREALAEGTGRRDIVEAHFDAFIYGTGLVDDRVASIQTAELLPQPGYW
                     THAGKQVLVGSKALAKAIEDRRKAWKAKHPDAKEWEGAFPYKTRVVVRRDGQKVPQVL
                     RVRFADGSKRDLPVTASGSWQRFEFVTASKAVSAQLDPDDLIRTDLSELNDSRTVEAD
                     GSAARRWFGDFTSLLQSLFALLSFV"
BASE COUNT         1354 a         2682 c         2763 g         1213 t
ORIGIN      
        1 ggcagcagcg ccagcgcggc gaccacgatc agcatccgcc accacaccag cggcagcgcg
       61 ggaagggtga tcagcttgcc gaggatcgcg gtgaagcccc acagcagcac gcaaagatgg
      121 atctgccaaa gggcgcgagt ggtgtcgcgg ttgctcatgc gcgcattatc gccgcacgga
      181 ggcgatgcca tgggccgcga gtacctcgat tacctgcacg acctgttctc ggcgttcgcg
      241 ccggtcacca cgcgcgcgat gttcggtggg catggcgtct atcgcgatgg ggtcatcatc
      301 gccatcgtca tcgatgaact cctctacctc aaggtcgacg accagacccg cgccgcgttc
      361 gaggccgaag gctgcgcgcc cttcgtgtac gaagcacagg gcaagcgcgt gccgatgagt
      421 tactggaccg tgccggacga agcgctggat tcgccgcagg agttcaggcc ttgggcgcag
      481 cgggcgtggg aggcggcgtt gcgcaagccc aaggcgaagc cgaagaagcg gaaggcatcg
      541 aagaggaaaa tggggtcaga gtagagtttt gcgggcgaga tgcatgagta gcggaaattc
      601 atcctcgcaa aatctactct gaccccattt tctgctcgct ggtcgccttg cgcccctgag
      661 cccgccctag ccagagcccc agcatcgccc aggccagcgc cagcggcacc accacggtga
      721 ccagcccgcc cagtgccatg cccagccgac caagcaggcc ctccacctgc gcacccgcca
      781 cgtcgccgcc gcgatagacg aaggtgtcga tgaccgcctt ggccttgtac ttgtcctcgc
      841 ggctgatcac ggtgaacagc gcctcgcgcg cggggcgggt gaggccgcgt tgcaccgcct
      901 tgttcagcgc ttccagcagc accagcgcgg cgaacgaccc gaagatcgcc aggccgatga
      961 agcccagcgc gttggcgatc ggcagcaacg ccagcgcgaa cgccaggccg aagcggcgga
     1021 tgacgtggcc ggtcagggtc agctgcaaca gcagcaccgc gacctgcgtc cacatgtcga
     1081 tgctggccag caagccggtg cgcgcgtcga ggtcgctctc cgcggcggcg accatctgca
     1141 ggcgggtgaa gtagacgaag gtggcgacga tggtcatcgc cagcacatag ccggcgatgc
     1201 ccaacagata aggcgatgaa aacaccgcgc gcaacccggc ccaggcgctg ccgccgatgc
     1261 gctgttcgcc gtcgcgcacg tcgtccacct gcgtcggggt ggtggcgcgg tcgccggcga
     1321 tccgcaccag ccaccaggcc agcgcgatgc ccaacaacag gaagcccgcc gcgaccagca
     1381 acagcgcagg cgtacccagc agctcggaaa gctgcgatgc gagccagggc ccgaagatcg
     1441 cgcccagggt gccgcccacc gcgatcagcg cgaacaggcg tttgccctgt tcgctgctga
     1501 agcgatcggc cagcagggcc cagaacacca tggtcacgaa caggttgaac acgctgaacc
     1561 agacatagaa cacctgcccg ctgcgtgcgc cgacggcgcc cggtgcgaac accagcaagc
     1621 cccagaaccc ggccagactc agcgcgaaga agccataggt cgcgctgatg aactgcaggc
     1681 gcctgaggcg gctcaccagc cagccgaaca gcgggttcac cagcagcgtg accacggcgg
     1741 tgccgatgaa cagccagcgg atgctctcga tgccgcgttc catgcccagt gcgtcgcgcg
     1801 ccggccgcag catctgcagc gcggtcagga tgcagaagaa gaacagcccg gcgaccagca
     1861 cgggcgccag ctcgccgcgc cggaggttga agaggccggc gatccgctgc tgcagccggg
     1921 cgtacctggg tcgatgcgtg tccatgccgc agacgatgcc gccgccaccg accgggggcg
     1981 aagtgccgga cggcacatgc cctccggcag ggatcagccc cgcgcgagca gtgcggtcaa
     2041 cgccatcgcc gccgccgggt tgcgttcctt ggccgcactg atgaactgga tgaagacgtc
     2101 gcgcggttcg tgcgtcggca ctgcgtcggt gatcttcggc aggtcgccag gctcgccgcc
     2161 gcttgcccac aggcgtgcgc gtgcggccca gccctcgagt tcgccaagcg ggtagccggt
     2221 ggcgatgtcg ctgcgcgaac gcatcagccg cgcgtagacg aactccgcgg tgacgtcggc
     2281 gaacgaggga tgttccggcg agtcggtgaa caccgtggcg aaaccatgct tgcgcgcaag
     2341 cgcaatgaaa tcggcatcga tgaaggccgc atcgcgcacg tcgaggacat gccgcaacgc
     2401 acgaccctcg accgtgcgtg gcagcaggtc gcagaaggct tcgaacgcat cgcgctgcag
     2461 cgtgagcccg gcatcgaact gccacagcag cgggcccagc ctgtcgccca gggtgccgat
     2521 gccaccgatg aaatcctcga cttgcggacc ggtcctggcc aatgccttcg actgcatgat
     2581 ccgcatcggt gccttcaccg agaagacgaa gccgtccggc gtttcgtcac gccatttcgc
     2641 atacgtcgcg ggtttctgcg cgccgtagta ggtgccgttg atctcgatgc agctcaggtg
     2701 ccggctggcg tattccagct cgcggcgctg gacgaggccc ttcggataga aattgtcgcg
     2761 ccacggcgca tacgtccagc cgccgatgcc gcaccggatg ccatcgacgg cgccaacccg
     2821 ttgcgaggtc cagtcgtcgg tatccgggaa caggctcatg acggcgactg ttccgcccac
     2881 ggatcgtagc tgccgaacca ccagtaatgc ccttccggat ccttgcacgc atacccacgt
     2941 ccgccgtacg gctggtcggc gatgtcgatg acgatggttg cgcctgccgc gactgcacgg
     3001 gcgtaatgcg catcggcatc ggccacgatc acgcaagcgc tctgggtttc gcggccgccg
     3061 atctcatcgg gctgcacgat cgccttgccc cattcgccgg cgttgctggt cgagccgagc
     3121 atcaccatcc cgttgccata caccagctgc gcgtgatgga cggtgtcgcc ctcggcatac
     3181 accgcgtgct gttggaagcc gaacgcctcg cacagccact cgatcatgcc cagcgcattg
     3241 cggtagcgca ggcagggaat gatggaggcc ctggtgttcg cgggctggga catggcggtg
     3301 ctccacggac gggacgtgcg agcgtacatc gctgggccga tgccggcagg cgtgacctgt
     3361 ggccatcgac gccgcccgcc gtgctggcga tactcggcgg gtcgccaacg aaggattccg
     3421 catgcgcctg cttccgatcg tcgtcgtcct ggccgtcgca cttgccggtg cgcccgtgca
     3481 ggcgcagcag tcgccgccac cgatccgcat cgacgacacc atggtgccgc tggctgcgcc
     3541 gctgccggat gcggcacgga tggcgcgctt cgatgccttc gtcgctgcgg tgcagaagca
     3601 gttcgacgtg cccggcgtgg cggtggccat cgtcaaggac gggcaagtcg tgctggagcg
     3661 cggctacggc gtgcgcaagc tcggcgaacc cgcgaaggtc gacgcgcaca ccatgttcgc
     3721 gatcgcgtcc aacaccaagg cgttcaccgc ggcggcgctg aacatgctgc aggacgaggg
     3781 caagctgaag accacggatc gcgtcatcga ccacttgccc tggttccgca tgagcgatcc
     3841 ctacgtgacc cgcgagatgc gcatccgcga cctgctggcg catcgcagcg gcctgagcct
     3901 gggcgcgggc gacctgctgt actggccgac cacgagctac accacgcggg aagtcgccga
     3961 gcgcttgaaa gacgtgccgc tcaccggcgg attccgagag cagtacgcct acgacaacat
     4021 cctgttcggc gtggcgcagc tggtcatcga agaagcctcc ggcatgccgt acaagcattt
     4081 ccttgaccgc cgcatcttcc agccgctggg catggccgag acccgctaca acagcgacga
     4141 cctcaagcct ggcgacaacg tggcgaccgg ccacgccaag ttcgatttca aggacctgcg
     4201 cccggtcggc gtgaccagct ggcgcaatgt gtccggcgcc ggcggcctct attccagcgt
     4261 gcatgacctg tcgaagtgga tgaacgtgca actcgcgggt ggtgtttacg cgggcagtgg
     4321 cgatgcggcg aaacgcctgt tttccgacgc gcgccagcgc gagatgtgga cggtgctgac
     4381 cccgatcccg gtcggcaagt cctcggtgcc cggcctggcc gcgatgaccc cgaatttcat
     4441 gggttacgga cagggctgga acctgtccga ttacgccggc gagcggctgg tctggcacac
     4501 cggcggctgg ccggggcagg tgtcgcggct gaccctggtg cccggcgaga agctcggcgt
     4561 ggtcgtgctc accagcggcg aggtcggcca ggccttccac gcgatcactt acgaagcgct
     4621 ggacatgatg ctgggccgca gcggcaatga ctggctgcag gcctacggag agtcgttcgc
     4681 ccgtgcgcaa ggcaatgccg acgaggactg gaagaagcat gtcgcggcgc gcgacgcgac
     4741 atccaaaccg tcactggcgc tggcgaacta cgccggcacc taccgcgatc cctggtacgg
     4801 cgacgtgctc atccgccagg gcgcgaaggg actggagatg cagttcacga agaccgcgga
     4861 actgctgggc gacatcgaac actggcagca cgacagcttc atcgtgcgct ggcgcgaccg
     4921 cggcctgaac gccgatgcct tcgtcaactt cagcctggac cccgacggcc gcatccgcga
     4981 actgcggatg gaaccgatct cgccgctgac cgacttcagc ttcgacttcc aggacctgcg
     5041 cctggccccg gtgaagtaac ccgtctccgg ccgtcaggca tcgcccccgg ccgccgcggc
     5101 ttcggcacgg gtgccgggct tgctggcgtg gcgggggatg tggtcctcgt ccagcagggc
     5161 ttcgatgcgg atgaagactt cggcgcgcga gaacggcttc ttcatgaagt cgtcggcgcc
     5221 gatgcgctgc acgtagaact gttcggtcgc ctgtgcgttg ccgctgatca tgatgatcgg
     5281 caggtgcttg gtgatcgggt cgcggcgcag ggtgcgcagg gcgttgaagc cgtccatccc
     5341 gggcaggacg atgtccagga agatgaggtc gggcacttcg cggcgggcga tctcgatgcc
     5401 gctttcggcg tcgaaggcct ccaggaccac cagttggttc tgctgcagca tgcgcttgag
     5461 cagggcgacg atcgtcggcg agtcgtcgat caccagcacg cgggtgccgg gccgggcgtg
     5521 gcggcggctt cgttcgcgac gttcggggcc gtcgtgctcg ggctgggcgg gttccgcgac
     5581 agttggagcg acttcggtat cgacttcggc ggggaactcg accggcgccg ggatcgcggg
     5641 ttcgcgcgcc gcaggtgcgg cggcgggcac ggatgcgggc gcttggttcg gcaccagccc
     5701 tggcggcaac accaatggct tgctggccgg cttggcggcc ggcttgcgct tgaaaagatc
     5761 gaagaaggcc acgccgttcc ccctggttgg ctcgtggaca atcttaaccg aacggcctgc
     5821 ccgggcggac ccgggcaggt ggcaggcgcg ggatcagcgg cgcgcgtgct ggtccgcggc
     5881 cagcgcggac atggcgtaca ggcgggcgat gcggccccag gcgagtcctg cggcgatgcc
     5941 gcaacccagc agcatcgcca gcaggaagcc gccgatgccg gcgccgccgg cgaactggcg
     6001 cagccaggcg aagagcaggg ccagcgcgag gcccagcgcc gtggtcacca gccaggccag
     6061 caacacggcg atggggcggc gggccagcag cttgaggcca cgccaccagg ccttgagggc
     6121 agatcgcagg cgaccgtcgg tggccagcca gccgcgcccg gcttccaggc tggcgtgggc
     6181 cagcacgaac agcacaccgc ccacgacgaa ggcgatgttg cggccggtcg tcaccgcgga
     6241 ggcttcgatc gcgtgttcat gcgcactttc gttggcgccg atgatcaccg acatcaccat
     6301 cagggccagg cccagcggga tcgccgacca cagcaacatc cgcagcagcg ggccgtactc
     6361 ggacagtgcg ccgcgcagca ggtcgccgaa accggggcgc gtgcgcgaac ggatcgcggc
     6421 gagggtggcg ccggccagca gcggcgagag cagcagcatc aggaccgcgg cgacggtggt
     6481 gctttcgccg agcacgccga gcggcgcggt gcgcgacatc agcaggtcga gcagcgggat
     6541 cggggcctgg ccgctggcga tcgccttggc ctgcaccgaa tggttgaggc cgtcggccag
     6601 ccagttccac gcgggcaggg cagcgatcag tgcgcaggcg aggctggcgc cgatccacag
     6661 cagccacagg cgccactgca tggcgccgcg caggccttcg ccaagggcgg agcggacgga
     6721 gagcgaagtc ggtcgggtgg tggacatggt cggcgcctca gacgaaggac agcagcgcga
     6781 acagcgactg caggagcgag gtgaagtcgc cgaaccagcg acgcgcggcg ctgccatcgg
     6841 cctcgacggt gcggctgtcg ttgagctcgg acaggtcggt gcggatcagg tcgtccggat
     6901 ccagttgcgc ggataccgcc ttggacgcgg tcacgaactc gaagcgctgc cagctcccgc
     6961 tggccgtcac tggcaggtcg cgcttgctgc cgtcggcgaa gcgcacgcgc agcacctgtg
     7021 gcaccttctg gccgtcgcgc cgcacgacca cgcgggtctt gtacgggaac gcgccttccc
     7081 attccttcgc gtccgggtgc ttggccttcc atgccttgcg gcggtcctcg atcgccttgg
     7141 ccaaggcctt gctgccgacc aggacctgct tgccggcgtg cgtccagtac cccggctgcg
     7201 gcaacagttc ggcggtctgg atgctggcca cgcggtcgtc gaccaggccg gtgccgtaga
     7261 tgaacgcatc gaaatgcgcc tcgacgatgt cgcggcggcc ggtgccctcg gccagcgctt
     7321 cgcgcaggtc ggccagcgac gggtggcgga acttccagcg ctcgtagtac agcttcatcg
     7381 cacgttccat cgccggggtg cccaccagcg cctcgatctg ccgcatcacc gtggcggtgc
     7441 gcgaatagac ggtgccatag ctgccgctgg agaggcgcga ccagctgttg tcgcccagcg
     7501 cgtcggccgg gtcggccagg ctggcgccca gtcgctccat gtcgaacggc gtcagcttgg
     7561 tgccgatccc gagcgcccgc aggaacggcg tggtcaggat caggtcctgc ttgcgcccgc
     7621 tcatcatccg ctggtcccaa tactcgttca tgccctcgtc gagcatgggt tcctcgaact
     7681 cgttggaggc caggatcccg tagaagtagc cgtggccgaa ctcgtgcacg gtgacgaaat
     7741 ccagggcgaa gcgaccgagc gtgcccggct cgtcctggct ggtgctgtcg gcggtgaaga
     7801 aggtcgggta ttccatgccg ccggcttcgt cggcgccgta cggcggcacc accgcggtgg
     7861 ccgtgccgta gggatatcca cccagcgtct tcgagaagta atcgagcgag tcgatggtcg
     7921 cctgcagcac cggttccgcg ttcgaggcgt aatcgggtgt gtacagcacg ctgacctgca
     7981 ccggcccgtt gggtccgttc caggtcttga cc
//
LOCUS       DAOOCX010000098         7968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_8556_length_7968_cov_7.840010, whole genome shotgun sequence.
ACCESSION   DAOOCX010000098 DAOOCX010000000
VERSION     DAOOCX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8556_length_7968_cov_7.840010"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1167
                     /locus_tag="PK399_09220"
     CDS             115..1167
                     /locus_tag="PK399_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPW13337.1"
                     /translation="MDSNAPTDHPPGDSRTIEQRAAEWLARREFNAWADEDEHALQAW
                     LDADTGHRVAFLRLQAAWSESDRLQALAAGWKQSGPPPRGYWTTPAGERIELTPRQPA
                     RRQRLPRFAAAAVLLIACTLVTGLGWRAYNRVDSAHLQTALGATGTLPMADGSQAILA
                     SDSRIDVRLSRRQRHVALEQGEAFFSVAKDPGRPFVVAAGGHDVAAVGTRFSVRRDGP
                     DLRVVVTEGTVRLESRATGAAARPASLLPAGSIAIVHGEDVLLRSVALEDAERLLDWR
                     QGLLAFRDTTLAEAAAEFNRYNARKLVIGDAEAGALRIGGSFRADNATGFVRLLEQGF
                     PVHAEERGERIVLHSR"
     gene            1256..4354
                     /locus_tag="PK399_09225"
     CDS             1256..4354
                     /locus_tag="PK399_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW13338.1"
                     /translation="MKPSRPLRALLLSLACTAALAAQAQTAPRVDIPAGDLAAALDAY
                     ARQSGTQLVYRADQLRGARSPGVQGQPASPQALDALLKGSGYRAQRDGSGAVVIVPQA
                     AAKPAAAAPARPRAAQAAPPPSTPADTPVTDLQTVQVTGSRIPRAQVEGPAPITLITA
                     EQIQAAGLTSVPDVLRSLSQNSGSVQGQQNTTSAQSTPGAQAVDLRGLGPNHTLVLIN
                     GRRIADFPLPLNSRSNFTDIGNIPLGMIDRVEVLTGSASAVYGSDAMAGVINFILKKS
                     TDGTIIDYRYGDTQRGGGESHRLSLTTGFERGDFSGIVGLELLDKRPLWGYDRDIQDS
                     TLDAPTSRRRLPRLVAQRYDWDDDVNIGPADDCAAMSGLNEGTTVLSEDRWGEPLCGS
                     ERAIAYRTIQNQRKGANVYGALEYRFSSGLAWFADFQLGRQTVKLLTGTNGNDVASDH
                     MGWEFHDPNSTDNNDKIFFNANTGHYEIWSRQFAPEEVGGLENRMNSTTQKTFAITTG
                     FNGTMGEDWTWEAAYNHSEYKAVVKMPRINAAAANRYFLGERLGYDDDGYAIYRPDPT
                     RLFTPLTPGDFASIGAMSTWHPVAKNDNVSFTANTPSLFTMPAGDVGFAGAIEYGNQS
                     YRINPDPLALTADAYYGPRYGDGRGSRDHWSAAGELRVPLLQSLQASVAGRYDMYSYG
                     DKDPGKFTYSLGLEWRPLDTLLVRGSYGTGFRAPDMHYLFAGNDYYRTVSTDYYQCRT
                     EEPGYSDADCYDDGSWDVNTFDVYKGNMQLDVETSKSFTAGFVWSPSPNFDLAVDYYQ
                     IRIANQVQSQSRELLRATEANCLLGVTDGGVAVDINSPTCVDALARVVREDPNDPFST
                     ITSVLFAPINIANEETSGIDVTANYRLQTASAGDFRFTGNYTWAHRHNRVLYPGDPEE
                     DMLDLSFSATTLPRTKGNVGVDWDKGAWGAGLFGNYVGRVANYNNDAWTGATWRFNGS
                     ARYDISDHLRVSLSVNNLLDKMPPRDATWANYPYYDTSWFDSMGRSYYLQLTWKLGGK
                     PL"
     gene            complement(4406..4972)
                     /locus_tag="PK399_09230"
     CDS             complement(4406..4972)
                     /locus_tag="PK399_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW13339.1"
                     /translation="MPRNRFVEGLGRTVLRLGGWRMVGTFPDLPKFVLIAAPHSSNWD
                     GVWGAAVKLAMGVKLSILGKEALVKAPLFGPLAKWQGVIGVDRSAAHGVVEQAVMAIR
                     NADRMWFTIAPEGTRKPVDRWKPGFWHIARAAGVPVVAAYFDYANKVVGIGPLFELTD
                     DMHADIARIQRWYKPYKGRNHDVQQPAD"
     gene            complement(5026..5439)
                     /gene="arfB"
                     /locus_tag="PK399_09235"
     CDS             complement(5026..5439)
                     /gene="arfB"
                     /locus_tag="PK399_09235"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HPW13340.1"
                     /translation="MQVGHLSIDDDELIERFVRASGPGGQNVNKVATAVELRFDVAQS
                     PSLPEPVRERLLAKRDRRMTNEGVLVLAAQRFRTQERNRQDARERLAHFIEGGLHAPK
                     PRIATKPSRGAKERRLGEKRGRATIKQGRTGRGWD"
     gene            5514..6035
                     /locus_tag="PK399_09240"
     CDS             5514..6035
                     /locus_tag="PK399_09240"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HPW13341.1"
                     /translation="MRLLGLAFALALSTAFATPAFAADPAPAPQQQASAADIDRLLEV
                     MDMETMMAGMMAQMSNVQAQMVDEAFGKELSDADRARMQSVTARTQALVEKSMSWTAI
                     EPIIRKVYLQLFSKPEVDAMIAFYGSTEGASILRKSPQAMTLTMQEMQPLMMRLMEDI
                     KVDIDNEVKARKD"
     gene            complement(6056..6799)
                     /locus_tag="PK399_09245"
     CDS             complement(6056..6799)
                     /locus_tag="PK399_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW13342.1"
                     /translation="MTDLAPDGPLADIGITLDLPVLFRDDALAVVCKPAGLMVHDSAL
                     ARGETDFAADRLREQFGKPIFLVHRLDRATSGCLLLAFDRETASALGKTLMSHEVEKD
                     YWAVCRGWPAEQVFTIDHPLDGGPGKPQKKAAITDFEVLGRCELSMPSAGFDTSRYAW
                     LRASPRTGRFRQIRRHLKHQFHHLIGDSSHGDGRHNRNFRMLGIHRMLLHARRLAFVH
                     PHTGERIETHAPVDAEFAKALALFDAAPR"
     gene            complement(6840..7781)
                     /locus_tag="PK399_09250"
     CDS             complement(6840..7781)
                     /locus_tag="PK399_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPW13343.1"
                     /translation="MGAGHDHKHDHERHHHGGAGHSHVPSEIKFERPLWIAFGLTLFF
                     LVVEVVGGLLTNSLALLSDAAHMMTDVLALGISLFAVRMARRPADAKRTYGYARMEAI
                     GALINGGLLFLVAGYILWEAIGRFSQPPHVESNGMLLVAVLGLVVNLVSMKLLQAGAG
                     ESLNMKGAYLEVWSDMLGSLGVIAGALLIRYTGWTVVDPVIAVLIGLWVLPRTWTLLR
                     AAGHVLMQGVPSGLDLDEIRDAMSWQPDVATVHDLHVWALGSKEPILTAHVLLANEDA
                     DADAVRNAMATMLHERFDIDHATLQVERRHCGGDLHP"
BASE COUNT         1355 a         2856 c         2580 g         1177 t
ORIGIN      
        1 tcagcgagaa gaccgtggaa aagcacatcg ccaagggcat gcgcctgctc gccgaccatc
       61 tgcatggcgg cggcgaggac acacgcatgc agaaacccgc cacagacaac cgtgatggac
      121 agcaacgcgc cgactgacca tccgcccggc gacagccgga cgatcgagca gcgcgcggcc
      181 gagtggctgg cacggcgcga gttcaacgcg tgggcggacg aggacgaaca cgcgctgcaa
      241 gcctggctgg atgccgatac cggtcaccgg gtcgcgttcc tgcgcctgca ggcggcctgg
      301 tcggaaagcg accggctgca ggcactggcc gcgggctgga aacaatcggg gccgccgcca
      361 cgcggctact ggacaacgcc tgccggcgaa cgcatcgaac tgacgccgcg ccagccggca
      421 cggcgtcagc gcttaccgcg tttcgccgcc gccgcggtgc tgttgatcgc gtgcacgctg
      481 gtcaccgggc tgggctggcg cgcgtacaac cgcgtcgaca gcgcgcacct gcagacggca
      541 ctgggggcca ccgggacctt gccaatggcc gacggctcgc aggcgatcct ggccagcgac
      601 agccgcatcg acgtgcgcct ctctcgccgc cagcggcatg tcgcgctgga acagggcgag
      661 gccttcttca gcgtggccaa ggatcccggc cgcccgttcg tggtcgccgc cggcgggcac
      721 gacgtggccg cggtcggcac ccggttctcg gtgcgccgcg acggccccga cctgcgggtg
      781 gtggtgaccg aaggcaccgt gcgcctggaa tcccgggcca ccggtgccgc cgcgcggccg
      841 gcctcgctgc tgcctgcggg cagcatcgcg atcgtccacg gcgaggacgt gctgctgcgc
      901 agcgtggcgc tggaggacgc cgagcgcctg ctcgactggc gccagggcct gctggccttc
      961 cgcgatacca cgctggccga ggccgccgcc gagttcaacc gctacaacgc gcgcaagctt
     1021 gtgatcggcg atgccgaggc cggcgcgctg cggatcggcg gcagcttccg cgccgacaac
     1081 gccaccggct tcgtgcgcct gctcgaacag ggcttcccgg tgcatgccga ggagcgcggc
     1141 gagcgcatcg tgctgcacag ccgttgacga tgccttgcat gccggcgggg ggatttcgcc
     1201 ggctcgttcg tcctgatcat cgaagcgccg cagacaaggc gcatggggaa ccgtcttgaa
     1261 accatcacgt ccgttgcgtg ccttgctgct gtccctggcc tgtaccgccg cgcttgccgc
     1321 gcaggcgcag accgcccccc gcgtggacat ccccgccggc gacctcgccg ccgcgctgga
     1381 tgcctacgcc cgccagtccg gcacccaact ggtgtatcgc gccgaccaac tccgtggcgc
     1441 gcgcagcccc ggcgtgcagg gccagccggc atcgccgcag gcgctcgacg ccctgctcaa
     1501 gggcagcggc taccgcgccc agcgcgatgg ctccggcgcc gtggtcatcg tgccgcaggc
     1561 ggctgcgaaa ccggcagcgg ccgcacccgc ccggccgcgc gccgcgcagg cggccccgcc
     1621 gccatcgacg cccgcggata cgccggtgac cgacctgcag accgtgcagg tcaccggttc
     1681 gcgcatcccg cgtgcgcagg tcgaaggccc ggcgccgatc accctgatca ccgccgagca
     1741 gatccaggcc gcgggcctga cctccgtgcc ggacgtgctg cgctcgctca gccagaacag
     1801 cggcagcgtg cagggccagc agaacaccac cagcgcgcag tccacgccgg gcgcgcaagc
     1861 cgtcgacctg cgcggcctcg gcccgaacca caccctggtg ctgatcaacg gccgccgcat
     1921 cgccgacttc ccgctgccgc tcaacagccg cagcaacttc accgacatcg gcaacatccc
     1981 gctgggcatg atcgaccggg tcgaagtgct caccggcagc gcctcggcgg tgtacggctc
     2041 ggacgcgatg gccggcgtga tcaacttcat cctgaagaag tccaccgacg gcaccatcat
     2101 cgactaccgc tacggcgaca cccagcgcgg cggcggcgaa tcgcaccggc tgtcgctcac
     2161 caccggcttc gagcgcggcg acttcagcgg catcgtcggc ctggaattgc tcgacaagcg
     2221 cccgctgtgg ggctacgacc gcgacatcca ggactcgacg ctggacgcgc cgacctcgcg
     2281 ccggcgcctg ccgcgcctgg tcgcgcagcg ctacgactgg gacgacgacg tcaacatcgg
     2341 cccggccgac gattgcgcgg cgatgtcggg cctcaacgaa ggcaccaccg tgctttccga
     2401 agaccgctgg ggcgaaccac tgtgcggcag cgagcgcgcg atcgcgtatc gcaccatcca
     2461 gaaccagcgc aagggcgcga atgtctatgg cgcgctggag taccgctttt ccagcgggct
     2521 ggcgtggttc gcggatttcc agctgggccg gcagacggtc aagctgctga ccggcaccaa
     2581 tggcaacgac gtggccagcg accacatggg ctgggaattc cacgacccca actcgaccga
     2641 caacaacgac aagatcttct tcaatgcgaa caccggccac tacgagatct ggtcgcggca
     2701 gttcgcgccg gaggaagtcg gcggcctcga aaaccgcatg aacagcacca cccagaaaac
     2761 gttcgcgatc accaccggct tcaacggcac gatgggcgag gactggacct gggaagcggc
     2821 ctacaaccac tccgaataca aggccgtggt gaagatgccg cgcatcaacg ccgccgcggc
     2881 caatcgctac ttcctgggcg aacggctggg ttacgacgat gacggctatg cgatctaccg
     2941 ccccgacccg acgcgcctgt tcaccccgct gaccccgggc gatttcgcca gcatcggcgc
     3001 gatgtccacc tggcacccgg tcgccaagaa cgacaacgtc agcttcaccg ccaacacgcc
     3061 gtcgctgttc accatgcccg ccggcgacgt cggcttcgcc ggcgcgatcg agtacggcaa
     3121 ccagtcgtac cggatcaacc cggatccgct cgcactcacc gcggacgcct actacgggcc
     3181 gcgctacggc gacggtcgcg gcagccgcga ccactggagc gcggcgggcg aattgcgcgt
     3241 gccgctgctg caatcgctgc aggcgagcgt ggccggccgc tacgacatgt acagctacgg
     3301 cgacaaggat ccgggcaagt tcacctacag cctgggcctg gaatggcgtc cgctggacac
     3361 cctgctggtg cgtggctcct acggcaccgg cttccgcgcg cccgacatgc actacctgtt
     3421 cgccggcaac gactactacc gcacggtctc caccgactac taccagtgcc gcaccgagga
     3481 acccggctac agcgatgccg actgctacga cgacggcagc tgggacgtga acaccttcga
     3541 cgtctacaag ggcaacatgc agctggacgt ggaaaccagc aagtcgttca ccgccggctt
     3601 cgtctggtcg ccctcgccga acttcgacct ggcggtggac tactaccaga tccgcatcgc
     3661 caaccaggtg cagtcgcaga gccgcgaact gttgcgggcg acggaagcga actgcctgct
     3721 cggcgtgacc gatggcggcg tcgccgtgga catcaattcg ccgacctgcg tggatgcgct
     3781 ggcgcgcgtg gtccgcgagg atcccaacga tcccttcagc acgatcacca gcgtcctgtt
     3841 cgccccgatc aacatcgcca acgaggaaac gtcgggcatc gacgtgaccg ccaactaccg
     3901 cctgcagacc gccagcgccg gcgatttccg cttcaccggc aactacacct gggcgcaccg
     3961 ccacaaccgc gtgctctacc cgggcgatcc ggaagaagac atgctggacc tgagcttcag
     4021 cgccaccacc ctgccgcgca ccaagggcaa cgtgggcgtg gactgggaca agggcgcgtg
     4081 gggtgccggc ctgttcggca actacgtggg ccgcgtggcc aactacaaca acgatgcgtg
     4141 gaccggcgcc acctggcgtt tcaacggcag cgcgcgctac gacatcagcg accacctgcg
     4201 ggtctcgctg tcggtcaaca acctgctcga caagatgccg ccccgggacg cgacctgggc
     4261 caactacccg tactacgaca cctcgtggtt cgacagcatg ggtcgcagct actacctgca
     4321 gctcacctgg aagctcggcg gcaagcccct ctgaacaccc cccgcgtcgt gacacttgga
     4381 gccccggcat gccggggctt ttttttcagt cggcgggctg ctgcacgtcg tggttgcggc
     4441 ccttgtaggg cttgtaccag cgctggatgc gggcgatgtc ggcgtgcatg tcgtcggtca
     4501 gttcgaacag cgggccgatg ccgaccacct tgttggcgta atcgaaatac gccgccacca
     4561 ccggcacgcc ggcggcgcgg gcgatatgcc agaagcccgg cttccagcgg tcgaccggtt
     4621 tgcgcgtgcc ttccggggcg atggtgaacc acatgcggtc ggcattgcgg atcgccatca
     4681 ccgcctgttc caccacgcca tgggcggcgc tgcgatcgac cccgatcacg ccctgccact
     4741 tggcgagcgg cccgaacagc ggcgccttca ccagcgcctc cttgcccagg atcgacagct
     4801 tcacgcccat cgccagcttc accgcggcac cccacacgcc gtcccagttg gacgaatgcg
     4861 gcgccgcgat cagcacgaac ttcggcaggt cgggaaacgt accgaccatg cgccagccgc
     4921 caagccgcaa cacggtgcgg cccagccctt ccacgaatcg gttgcgcggc atccgcggcg
     4981 cattgggcgg caatgccacc accacgtcgt catgtgcttg cgcgttcaat cccaaccccg
     5041 tcccgtgcgg ccctgcttga tcgtcgcacg cccgcgcttc tcgcccaacc ggcgttcctt
     5101 cgccccgcgc gatggcttgg tggcgatgcg cggcttgggc gcatgcagcc cgccttcgat
     5161 gaaatgcgcc agccgctcgc gtgcatcctg tcggttgcgc tcctgggtgc ggaagcgctg
     5221 cgcggcgagc accagcacgc cctcgttggt catgcgccgg tcgcgcttgg ccagcaggcg
     5281 ctcgcgcacc ggctccggga gcgagggcga ctgcgccacg tcgaagcgca attcgaccgc
     5341 ggtggcgacc ttgttgacgt tctgcccacc cggccccgac gcccgcacga agcgttcgat
     5401 gagttcgtcg tcgtcgatgg aaaggtggcc gacctgcatg gcgcaattgt agggcggttg
     5461 ccattcgccg accgggttgg gacgatgccg caccttttcc cccggaaccc gccatgcgcc
     5521 tgctcggcct tgccttcgcc cttgccctgt ccaccgcgtt cgccacgccg gccttcgccg
     5581 ccgatccggc acccgcgccg caacaacagg cctccgccgc cgacatcgac cgcctgctgg
     5641 aggtgatgga catggagacg atgatggccg gcatgatggc gcagatgtcc aacgtgcagg
     5701 cgcagatggt cgacgaggcc ttcggcaagg aactgtccga cgccgaccgt gcgcgcatgc
     5761 aatcggtcac cgcgcgcacc caggccttgg tggagaagag catgtcctgg accgccatcg
     5821 aaccgatcat ccgcaaggtc tacctgcagc ttttcagcaa gccggaagtg gacgcgatga
     5881 tcgcgttcta cggcagcacc gaaggcgcct cgatcctgcg caagtcgccg caggcgatga
     5941 ccctgaccat gcaggaaatg cagccgctga tgatgcgctt gatggaagac atcaaggtcg
     6001 acatcgacaa cgaagtgaag gcgcgcaagg actgacggcg gcgccgtagc cggcttcaac
     6061 gcggcgcggc gtcgaacagc gccagcgcct tggcgaattc cgcatccacc ggcgcatgcg
     6121 tttcgatgcg ttcgccggtg tgcgggtgca cgaacgccag ccgccgtgca tgcagcagca
     6181 tgcgatggat gccgagcatg cggaagttgc ggttgtggcg gccatcgccg tggctgctgt
     6241 cgccgatcag gtgatggaac tggtgcttga gatggcggcg gatctggcgg aaacggccgg
     6301 tccgtggcga ggcgcgcaac caggcatagc gcgaggtgtc gaagccggcg gagggcatcg
     6361 acagctcgca gcggcccagc acctcgaagt cggtgatcgc cgccttcttc tgcggcttgc
     6421 cgggtccgcc atcgagcgga tgatcgatgg tgaacacctg ctccgccggc cagccgcggc
     6481 agaccgccca gtagtccttc tcgacctcat gcgacatcag cgtcttgccg agcgcggaag
     6541 cggtttcgcg gtcgaaggcc agcaacaggc agccgctggt ggcgcgatcc agccgatgca
     6601 ccaggaagat cggcttgccg aattgctcgc gcaggcggtc ggcggcgaag tcggtttcgc
     6661 cgcgtgccag cgcactgtca tggaccatca gcccggccgg cttgcacacc accgcgagtg
     6721 cgtcgtcgcg gaacagcacc ggcaagtcga gggtgatgcc gatgtcggcg agcgggccat
     6781 cgggcgcgag gtcggtcatg cgccattgtc ccgcatcgcg ccggcaccga ggcgcttgct
     6841 caggggtgca ggtcgccgcc gcaatgccgg cgttccacct gcagggtggc gtggtcgatg
     6901 tcgaagcgct cgtgcagcat cgtcgccatc gcgttgcgca ccgcatccgc atcggcgtcc
     6961 tcgttcgcca gcaacacatg cgcggtgagg atcggctcct tcgaccccaa cgcccagaca
     7021 tgcaggtcgt gcaccgtggc gacgtcgggt tgccaggaca tggcatcgcg gatctcgtcc
     7081 aggtccaggc cggaaggcac gccctgcatc agcacgtggc cggcggcgcg cagcagcgtc
     7141 cacgtgcgtg gcaagaccca caacccgatc aacaccgcga tgaccggatc gaccacggtc
     7201 cagccggtgt aacggatcag caacgcgccg gcgatcacgc cgagcgaacc gagcatgtcg
     7261 ctccacactt ccaggtaggc acccttcatg ttcaggcttt cgcccgcgcc ggcctggagc
     7321 aacttcatcg agaccaggtt gacgaccagg cccagcacgg ccaccagcaa catgccattc
     7381 gactcgacat gcggcggctg gctgaagcgg ccgatcgctt cccagaggat gtagccggcg
     7441 accaggaaca gcaggccacc attgatcagc gcaccgatcg cttccatccg cgcatagccg
     7501 taggtgcgct tcgcgtccgc cggcctgcgc gccatccgca ccgcgaacag cgagatcccc
     7561 agcgccagca cgtcggtcat catgtgcgcg gcatcggaca gcagggccag gctgttggtc
     7621 agcaggccgc cgaccacttc caccaccagg aagaacaggg tcaggccgaa cgcgatccac
     7681 agcggccgct cgaacttgat ctcgctgggc acatggctgt gcccggcgcc gccgtggtgg
     7741 tgccgctcgt gatcgtgctt gtggtcgtgg cctgcgccca tccgcctgca accgtgcttc
     7801 gccaggccga accctagcga tggatgcgcg ggttacacaa tctcacttcg ccggtgcggc
     7861 ctcgtccgca cccagcaacg gcagcacctg ttgcagcaat ttcgccgtga tctggtgcgg
     7921 ctggcgctcg ttgtagttgg tggtggtcac caccacgacc acgcgctg
//
LOCUS       DAOOCX010000099         7817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_8785_length_7817_cov_8.358284, whole genome shotgun sequence.
ACCESSION   DAOOCX010000099 DAOOCX010000000
VERSION     DAOOCX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8785_length_7817_cov_8.358284"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PK399_09255"
     CDS             <1..567
                     /locus_tag="PK399_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPW13344.1"
                     /translation="GGEYGPRWHQAALKANRHKAYEDFAAIADDLVARKITSPKHLGT
                     MGGSNGGLLMGNMLTQYPEKFGAIVVQVPLLDMKRYSHLLAGASWMAEYGDPDTADWS
                     FIQTFSPYHLFDAKKDYPPTIFMTSTKDDRVHPAHARKMMAMMEAAGKDVRYYENIEG
                     GHGGAANNAQSAHMTALAYTFLWQQLAK"
     gene            714..2876
                     /locus_tag="PK399_09260"
     CDS             714..2876
                     /locus_tag="PK399_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPW13345.1"
                     /translation="MKPQALIASLFLMTSIASAQTPPSPPDVAKKPHVVKAPHGAERQ
                     DEYYWLRDDKREDKAMLGYLEAENAYADTVLAPLKPVQGKLYDEIVARIKQDDASVPF
                     RERGWWYYSRFEAGKDYPIHARRKDAAGIDAGAILKANEAADFAGEEVLLDVNAMATG
                     KDYYAVSGRTVSQDNRWLVYGDDTNGRRQYTLRIKDLQTGKVLDDAIPGTAGYGVWAD
                     DNRTFFYVENDPETLLSTKVKKHVVGTDPKDDVLVYEEQDDTYYMGIGRTRDDQYIVI
                     ALDSTVSNETRYASAANPDAFKVLAPRARDVEYDADHHGGRWVIRTNDGGAKNFKIVT
                     AADGSTSRKDWKDWIAHDPQVYVEGIELFDGFAAVAERSGGLERVRVLKDGGGSEYVK
                     ADESAYSMGLDVNAETDTDWLRYSYTSLAMPATTYELNVKTGERRELKRQPVPGYDPA
                     NYVTERLWATARDGVKVPVSIVYKKGFAKDGKGALLQYGYGSYGASMDPGFSVTTVSL
                     LDRGMAYAIAHIRGGQEMGRGWYEDGKLLHKKNTFTDFIDVTDFLVKEGYAAPDRVAA
                     LGGSAGGLLMGAVANMAPEKYSVILSQVPFVDVVTTMLDASIPLTTNEYDEWGNPEQK
                     KYYDYMLTYSPYDNLKAQAYPATFVGTGLWDSQVQYWEPAKYVARLRDLNTSSEPVVF
                     RTNMEAGHGGKSGRFRRYREQAEMYSFMLDQLGVDAGK"
     gene            2997..3359
                     /locus_tag="PK399_09265"
     CDS             2997..3359
                     /locus_tag="PK399_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007965023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HPW13346.1"
                     /translation="MTRWLWWLLAYASLGIGIVGIVVPGLPTVPFVLLAAFAAARGSQ
                     RLHAWLLAHRQFGPMILDWEREGAVSRRAKRLAIGMMAACAVVMFLTAPQWWMAATGT
                     AFMAIVATWLWRRPEPRQ"
     gene            3385..3561
                     /locus_tag="PK399_09270"
     CDS             3385..3561
                     /locus_tag="PK399_09270"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HPW13347.1"
                     /translation="MIKRLAFATFLLCILGACGNKGPLVMPDPPADLPAAPADAPAPA
                     TTPEGASGTPAPRR"
     gene            3569..4435
                     /gene="dapF"
                     /locus_tag="PK399_09275"
     CDS             3569..4435
                     /gene="dapF"
                     /locus_tag="PK399_09275"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPW13348.1"
                     /translation="MPLHFTKMHGAGNDFVVLDLRDGQSPPDPATCARIADRHRGVGC
                     DQLLTVESPREAGSIASYRIWNADGSPSGQCGNGARCIAAWLVRDGAAPRQGGFAIDS
                     PARTHRVEAGDDGGFTVEMGAPRFEPAEIPLAGFAHMQDDYVVQLANGERIEHLRFGA
                     VSMGNPHAVVEVADVDAADVARIGPALQRHAAFPASANVGFAQVLSPGHIRLRVYERG
                     VGETLACGSGACAAVAVLAARGRVDGDREVIVDLPGGRLRIRHDRQAGTMSMGGPAAF
                     VFEGTMQQGSGA"
     gene            4438..5121
                     /locus_tag="PK399_09280"
     CDS             4438..5121
                     /locus_tag="PK399_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HPW13349.1"
                     /translation="MGYDDNNEKLGAHEVATWLRRHPAFLQQFPDLALTLVVPREDGP
                     AASLASYQLDVLRDKNRELSRRLHDLYGNAQENERLAVRTHQLTLALMKQANAADTLR
                     AMAASLAEDFQGDLVRIVAFHPVDGVGDVDWLQVIAQDDPRLAGFRDILGSGEPVCGR
                     LHPDKNALLYGPRVDEVQSSALLSLPGVGLVAVGSRDPNRFFPGMGTLFLRMMGEALA
                     AGLQRFPRG"
     gene            5123..6013
                     /gene="xerC"
                     /locus_tag="PK399_09285"
     CDS             5123..6013
                     /gene="xerC"
                     /locus_tag="PK399_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPW13350.1"
                     /translation="MNATGPVEAFLAHLSVERQGSAHTLDAYRRDLAALQAWADANGR
                     VLLDLQGDDLRAFIASEHRRGLSPKSLQRRLSACRSLYRWLLKNGQVAASPAEAIRAP
                     KAPRKLPQVLDVDEAVQLVEVATDAPLGLRDRALLELFYSSGLRLAETCALRWADLDF
                     DQGLVSVLGKGSKQRIVPVGSHARAALQAWRDESGGTDASPVFPGRGGKPITPRAVQL
                     RLRQLAMRQGVFKRVHPHLLRHSFASHMLESSGDLRGVQELLGHADIATTQIYTHLDF
                     QHLAKVYDAAHPRAKRKSGG"
     gene            complement(6137..6919)
                     /locus_tag="PK399_09290"
     CDS             complement(6137..6919)
                     /locus_tag="PK399_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13351.1"
                     /translation="MSAPTLQALVPVRGDAPFLAAALHSLRAQTFIDWQATLCPLDAN
                     ARDIAMRAVDGDARMQIADTSDMAEASALAHAADTNACELLCVLDGDDTLEPTAFAAL
                     MDALAEHPAAGMAYSRHVLIDANGGVLGPGPLCELPYSADALLLDFMTGPLRVLRRQA
                     YRDAGGFASAHADASDYDLCLRLSETAGVVHVPQALYQRRIHPLAPEIARWAEAIEAR
                     YGAFVDAVKRRGLDATYDVALHIDSWHILQPLRAFGGVGNWG"
     gene            complement(6916..7704)
                     /locus_tag="PK399_09295"
     CDS             complement(6916..7704)
                     /locus_tag="PK399_09295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW13352.1"
                     /translation="MPDLISIAVPIYNTARFLPAALDSLLAQDYPHWECLLWDDGSTD
                     GSGDIAAGYAVRDPRFRVLGDGRNRGVSATCAAALSEARGEYVAILDSDDMLEPDALS
                     SMLAFMHANPRLGMAYSEYVEVAEDGRYLAPGRRFQRPYSAHNLLVDFITYQFRLIRA
                     EAYRTVGGVDASMPVAYDYDLCLRLSERVEVGHLPKPLYRYRMRKESISHASRLRQVR
                     ASFDAAQRALQRRGLQGKYALALGVRARHVLRPKDASSPQGEGA"
BASE COUNT         1338 a         2615 c         2668 g         1196 t
ORIGIN      
        1 ggtggcgagt acggcccgcg ctggcaccaa gccgcgctga aggccaaccg ccacaaggcg
       61 tatgaagact tcgcggcgat cgccgacgac ctggtcgcgc gcaagatcac ctccccgaag
      121 cacctgggca cgatgggcgg cagcaacggc ggcctgctga tgggcaacat gctcacccag
      181 tacccggaga agttcggcgc gatcgtggtg caggtgccgc tgctcgacat gaagcggtac
      241 agccacctgc tggcgggtgc ctcgtggatg gccgaatacg gcgatccgga caccgccgac
      301 tggtcgttca tccagacgtt ctcgccgtac cacctgttcg atgcgaagaa ggactatccg
      361 ccaaccatct tcatgacatc gaccaaggac gatcgcgtgc atcccgcgca cgcgcgcaag
      421 atgatggcga tgatggaagc tgccgggaag gacgtgcgct attacgagaa catcgagggt
      481 ggccacggcg gcgcggcgaa caacgcgcag tccgcgcaca tgaccgcgct ggcttacacg
      541 ttcctctggc agcaattggc gaagtaatcg actccctccc ttgcgaaggc aggggagggc
      601 tggggtgggg tgctcttcct ccgacgcgaa agccaacccc ctcccaacct ccccctgcaa
      661 gcagggggag gagtgcaggc agcgttgcaa accgttccac ggagccccat tccatgaagc
      721 cccaagccct tatcgcaagc ctcttcctca tgaccagcat cgccagcgcg caaaccccgc
      781 ccagcccgcc cgacgtggcg aagaagccgc acgtggtgaa ggcgccgcat ggggccgagc
      841 gccaggacga gtactactgg ctgcgcgacg acaagcgcga ggacaaggcg atgctcggct
      901 atctggaggc cgagaacgcc tatgccgata ccgtgcttgc cccgctcaag ccggtgcagg
      961 gcaagctcta cgacgagatc gtggcccgca tcaagcagga cgatgccagc gtgccgttcc
     1021 gcgagcgtgg gtggtggtat tacagccgtt tcgaggcggg caaggactat ccgatccacg
     1081 cgcgccgcaa ggacgccgcc ggcatcgatg ccggcgcgat cctcaaggcc aacgaggccg
     1141 ccgatttcgc cggcgaagag gtgttgctgg acgtcaacgc gatggcgacc ggcaaggact
     1201 actacgcggt cagcgggcgc acggtgagcc aggacaaccg ctggctggtg tatggcgacg
     1261 acaccaatgg ccgccgccag tacaccctgc ggatcaagga cctgcagacc ggcaaggtgc
     1321 tggacgatgc cattcccggc actgccggct acggcgtgtg ggccgacgac aaccggacgt
     1381 tcttctacgt cgagaacgat ccggaaaccc tgctcagcac caaggtgaag aagcatgtcg
     1441 tcggcaccga tccgaaggac gacgtgctgg tgtacgaaga acaggacgat acctactaca
     1501 tgggcatcgg ccgcacccgc gatgaccagt acatcgtcat cgcgctggac agcacggtgt
     1561 cgaacgagac gcgctacgca tcggccgcga atccggacgc attcaaggtg ctggccccgc
     1621 gcgcgcgcga cgtcgaatac gacgccgacc accatggcgg tcgctgggtg atccgcacca
     1681 acgacggcgg tgcgaagaac ttcaagatcg tcactgccgc cgatggcagc acctcgcgca
     1741 aggactggaa ggactggatc gcgcatgacc cgcaggtcta tgtcgagggc atcgaactgt
     1801 tcgatggttt cgccgcggtg gccgaacgct ccggcggcct ggagcgcgtg cgcgtgctca
     1861 aggatggcgg cggctccgag tacgtgaagg ccgacgagag cgcctactcg atgggcctgg
     1921 acgtcaacgc cgaaaccgac accgactggc tgcgctacag ctatacctcg ctggcgatgc
     1981 cggcgacgac ttacgagctc aacgtgaaga ccggcgagcg gcgcgaactc aagcgccagc
     2041 cggtgccggg ctacgacccc gcgaactacg tgaccgaacg cctgtgggcg accgcacgcg
     2101 acggcgtcaa ggtgccggtc tcgatcgtct acaagaaggg gttcgccaag gacggcaagg
     2161 gcgcgctgct gcagtacggc tacggcagct acggcgcgtc gatggatccg gggttcagcg
     2221 tcaccacggt cagcctgctc gatcgcggca tggcttacgc catcgcccac atccgcggtg
     2281 gccaggaaat gggccgcggc tggtacgagg atggcaagct gctgcacaag aagaacacct
     2341 tcaccgactt catcgacgtc accgatttcc tggtgaagga aggctatgcc gcgccggatc
     2401 gcgtggccgc gcttggcggc agcgccggcg gcctgctgat gggggcggtc gccaacatgg
     2461 cgccggagaa atacagcgtg atcctgtcgc aggtgccatt cgtggacgtg gtcaccacga
     2521 tgctggacgc aagcattccg ctgaccacca acgaatacga cgagtggggc aacccggagc
     2581 agaagaagta ctacgactac atgctgacct actcgccgta cgacaacctg aaggcacagg
     2641 cgtatccggc gaccttcgtc ggcaccggcc tgtgggattc gcaggtgcag tactgggagc
     2701 cggcgaagta cgtcgcgcgc ctgcgcgacc tcaacacgtc gagcgaaccg gtggtgttcc
     2761 gcaccaacat ggaagctggc cacggcggca agtcgggtcg cttccgccgc tatcgcgagc
     2821 aggcggaaat gtattccttc atgctcgacc agttgggtgt cgacgccggc aaatgatgct
     2881 tgtctccctc ccttgcgaat gcaggggagg gttggggtgg ggtgctcttc ttgcgacggg
     2941 tagaaggcaa ccccctccca gcctccccct gccttcgcag ggggaggagc aaagccatga
     3001 cccgctggct gtggtggttg ctggcctatg ccagcctcgg gatcggcatc gtcgggatcg
     3061 tggtgccggg cctgccaacc gtgcccttcg tgctgctggc ggcgtttgcg gccgcgcgtg
     3121 gttcgcaacg gctgcatgcg tggctgctgg cgcatcgcca gttcggtccg atgatcctgg
     3181 actgggaacg cgaaggcgcg gtcagccggc gcgccaagcg cctggccatc ggcatgatgg
     3241 cggcgtgcgc ggtggtgatg ttcctgactg caccgcaatg gtggatggcc gccaccggca
     3301 ccgcgttcat ggcgatcgtc gcgacgtggc tgtggcggcg tcccgagccg cgacagtgag
     3361 caaggccgcg ggtacactgc cggcatgatc aagcgcctcg cattcgccac gttcctgctc
     3421 tgcatcctgg gggcctgcgg caacaagggc ccgctggtga tgccggatcc accggccgac
     3481 ctgcccgccg cgcctgcgga cgcgcccgcg cctgccacga ctccggaagg cgccagcgga
     3541 acgcccgccc cgcgccgctg aggcggcgat gccgctgcac ttcaccaaga tgcatggcgc
     3601 cggcaacgac ttcgtcgtgc tggacctgcg cgatggccaa tcgccaccgg atcccgccac
     3661 ctgcgcacgc atcgccgacc gtcacagggg cgtgggctgc gaccagttgc tgaccgtcga
     3721 atcgccgcgc gaagccgggt cgatcgcgtc gtaccgcatc tggaatgcag acggttcgcc
     3781 gtcgggccaa tgcggcaacg gcgcgcgctg catcgccgcc tggctggtgc gcgatggcgc
     3841 ggcaccgagg cagggggggt tcgccatcga cagtcccgcg cgcacgcatc gggtcgaggc
     3901 aggcgatgac ggtgggttca ccgtggagat gggcgcgccg cgcttcgaac ccgccgagat
     3961 cccgctggcc ggtttcgccc acatgcagga cgactacgtg gtgcagctcg cgaacggcga
     4021 gcgcatcgag cacctgcgct tcggcgcggt gtcgatgggc aatccgcatg cggtggtcga
     4081 agtcgccgat gtggacgccg ccgacgtcgc ccgcatcggc cccgcattgc aacggcatgc
     4141 ggcgttcccg gcgtcggcga acgtcggttt cgcgcaggtg ctgtcgcccg ggcacatccg
     4201 cctgcgcgtg tacgagcgcg gcgtgggcga aaccctggcc tgcggcagtg gtgcctgcgc
     4261 cgcggtcgcg gtgctggcgg cgcgcggccg cgtggatggc gatcgcgagg tgatcgtcga
     4321 cctgcccggc ggtcgcctgc gcatccgcca tgatcgccag gccggaacga tgtccatggg
     4381 cggcccggcc gccttcgtgt tcgaaggaac gatgcagcaa gggagcggtg cgtgacgatg
     4441 ggctatgacg acaacaacga gaaactcggt gcgcacgaag tggccacgtg gctgcgccgg
     4501 cacccggcct tcctgcagca gttccccgac ctggcgctga ccctggtggt cccgcgcgag
     4561 gacggccctg ccgcatcgct ggccagctac cagctggacg tgctgcgcga caagaaccgc
     4621 gaactgtcgc ggcgcctgca cgacctctac ggcaatgcgc aggagaacga gcggctggcg
     4681 gtgcgcacgc accagctgac cctggcgctg atgaagcagg ccaacgccgc cgataccctg
     4741 cgtgccatgg ccgcatcgct ggcggaagac ttccagggcg acctggtgcg gatcgtcgcg
     4801 ttccatccgg tcgatggcgt cggcgatgtc gactggttgc aggtgatcgc gcaggacgat
     4861 ccgcgcctgg ccggcttccg cgacatcctc ggcagcggcg aaccggtctg tggccgcctg
     4921 catcccgaca agaacgccct gctgtacggt ccgcgcgtgg acgaggtgca gagctccgcg
     4981 ctgctgtccc tgcccggtgt cggcctggtc gccgtgggca gccgcgatcc caatcgcttc
     5041 ttccccggca tgggcacgct gttcctgcgc atgatgggcg aggcgctggc ggccgggctg
     5101 caacggttcc cgcgcggatg acatgaacgc gacgggtccg gtcgaggcct tcctcgcgca
     5161 cctgtcggtc gagcggcaag gctcggcgca tacgctcgat gcctatcgtc gcgacctcgc
     5221 cgcgttgcag gcctgggccg acgccaacgg ccgcgtgctg ctcgacctgc agggcgacga
     5281 cctgcgcgcc ttcatcgcgt ccgaacaccg ccgcggcctg tcgccgaagt cgctgcaacg
     5341 ccggctttcg gcgtgtcgca gcctgtatcg ctggctgctg aagaacggcc aggtcgcggc
     5401 cagcccggcg gaggcgatcc gcgcgccgaa agcgccgcgc aagctcccgc aggtcctcga
     5461 cgtggacgaa gccgtgcaac tggtggaagt cgccaccgat gcgccgctgg gcctgcgcga
     5521 ccgcgccctg ctggagctgt tctattcgtc cgggctgcgc ctggcggaaa cctgcgcgct
     5581 gcgctgggcc gacctcgatt tcgaccaggg cctggtcagc gtgctcggca agggcagcaa
     5641 gcagcgcatc gtgccggtgg gttcgcatgc gcgcgcggcc ttgcaggcgt ggcgcgatga
     5701 atcgggcggc acggatgcat cgccggtctt ccccggtcgc ggcggcaagc cgatcacccc
     5761 acgtgcagtg caattgcgcc tgcgccagct ggcgatgcgc cagggcgtgt tcaagcgcgt
     5821 gcatccgcac ctgctgcgcc atagtttcgc cagccacatg ctggaatcct cgggcgacct
     5881 gcgcggtgtg caggaactgc tgggccatgc cgacatcgcc accacccaga tctacaccca
     5941 cctggatttc cagcacctgg ccaaggtcta cgacgccgcg catccgcggg cgaagcggaa
     6001 gtcgggtggc tgagtgccgg gcgaggttct tgtgggagag gcttcagccc cgaagctttc
     6061 ctgcagtcca ggacaaggcc cggggctgaa gcccctccca caacagcagg caggtagctg
     6121 cccgcaaggg gtggggctaa ccccaattcc ccaccccgcc gaacgcgcgc agcggctgca
     6181 ggatgtgcca gctgtcgatg tgcagggcga cgtcatacgt cgcatccagg ccgcgtcgct
     6241 tcaccgcatc gacgaacgcg ccgtagcggg cctcgatggc ttccgcccac cgcgcgattt
     6301 ccggtgccag cggatggatg cgacgctggt acagcgcctg cggcacgtgc acgacgccgg
     6361 cggtttccga cagtcgcagg cacaggtcgt agtcgctcgc gtccgcatgc gcgctggcga
     6421 agccgccggc atcccgatac gcctgccgac ggaggacccg cagcggcccg gtcatgaaat
     6481 ccagcagcag cgcgtcggcc gagtacggca attcgcacaa ggggcccggg ccgagtacac
     6541 caccgttggc atcgatcagc acatgccggc tgtaggccat gccggcagcc ggatgttccg
     6601 ccagtgcatc catcagcgcg gcgaacgcag tcggctccag cgtgtcgtcg ccatccagca
     6661 cgcacaggag ttcgcaagca ttggtgtccg cggcatgcgc gagtgcgctc gcttcggcca
     6721 tgtcactggt gtcggcgatc tgcatgcgcg catcgccatc gacagcacgc atcgcgatat
     6781 cgcgtgcgtt cgcatcgagt gggcacagcg tggcctgcca atcgatgaac gtttgtgcgc
     6841 gcaggctgtg cagcgccgcg gcgagaaacg gtgcatcacc acgcaccggc accagggctt
     6901 gcagtgtcgg tgcgctcatg caccttcacc ttgtggcgat gaagcatcct tcggccgcaa
     6961 cacatggcgt gcacgcacgc ccagcgccag cgcgtacttg ccctgcaggc cgcggcgctg
     7021 caacgcgcgc tgcgcggcat cgaagctcgc gcgcacttgc cgcaggcgac tggcatggga
     7081 aatcgactcc ttgcgcatgc ggtagcggta cagcggcttc ggcagatggc cgacctcgac
     7141 ccgctcggac aggcgcaggc agaggtcgta gtcgtaggca accggcatgc tggcgtccac
     7201 gccgcccacc gtgcgatagg cctcggcgcg gatcagcctg aactggtagg tgatgaagtc
     7261 gaccagcagg ttgtgcgcgg aatacggccg ctggaagcgt cggcctgggg ccaggtagcg
     7321 gccgtcctcg gcgacctcca catactccga atacgccatg ccgagtcgcg ggttggcgtg
     7381 catgaaggcc agcatcgacg acagcgcatc cggctccagc atgtcgtcgc tgtcgaggat
     7441 cgcgacgtac tcgccgcggg cctcggacag ggcggcggcg caggtcgcgc tgaccccgcg
     7501 attgcggcca tcgccgagca cgcggaagcg tggatcgcgc acggcgtaac cggcggcgat
     7561 gtcgccggag ccgtcggtcg atccgtcatc ccagagcagg cattcccaat gcgggtagtc
     7621 ctgcgccagc aggctgtcca gcgccgccgg caggaagcgg gcggtgttgt agatggggac
     7681 ggcgatgctg atcaggtccg gcatgtcgcc agtgtgcccg aaggcatgct tgaagcgcgc
     7741 ggccgtgtcc ccacatcggg gtttcacagg agatgccgat ggaccccagc cagaatccca
     7801 ccgtgttcca tgccacc
//
LOCUS       DAOOCX010000100         7774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_8852_length_7774_cov_7.621194, whole genome shotgun sequence.
ACCESSION   DAOOCX010000100 DAOOCX010000000
VERSION     DAOOCX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8852_length_7774_cov_7.621194"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /gene="aspS"
                     /locus_tag="PK399_09300"
     CDS             <1..1151
                     /gene="aspS"
                     /locus_tag="PK399_09300"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPW13353.1"
                     /translation="MAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFAWVGERDVQD
                     LTEDMIRHVFREVMGVELAAAFPRMTYAEAMRRYGSDKPDLRIPLEFVDVAEQVKGCE
                     FAVFTSAANDPDGRVVALRIPGGASLSRKQIDDYAAHAAKFGAKGLAYAKIDEAGAVN
                     SPVAKFFAEGAFDALLRHVGAGNGDIVFFGAGAYNKTSDFMGAVRLKAGKDFNLVADG
                     WAPLWVTDFPMFEWDDEEQRYVALHHPFTAPAVDSIDDLRANAKTAVSRGYDMVLNGN
                     EIGGGSIRIHNSAMQSAVFELLGIGAEEAEGKFGFLLDALKYGAPPHGGIAFGIDRIA
                     ALMAGSDSIRDVIAFPKTTTAQCLMTGAPSEVPDKQLAEVHVSVRARE"
     gene            1270..1797
                     /locus_tag="PK399_09305"
     CDS             1270..1797
                     /locus_tag="PK399_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW13354.1"
                     /translation="MKEATIRRATSDDAPALAELGATTFIEAFGQLYSPEDLQAFLEE
                     SHSLAAYAKVLANPDYALWIAEKDGRAIGYAQAGPCGLPHDDVRPGDGELKRLYLLRG
                     EQNGGVGRALFEQALAWLERDGPRTLWISVWSENYGAQRFYGRHGFEFAGEYAFIVGE
                     QRDREFMYRRDPRNG"
     gene            complement(1859..2287)
                     /locus_tag="PK399_09310"
     CDS             complement(1859..2287)
                     /locus_tag="PK399_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010626801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HPW13355.1"
                     /translation="MSIETVLYSATATATGGREGRAVSSDGVLDVALTVPKGLGGAGN
                     PGTNPEQLFAAGYSACFLGALKHVAGKQKVALPADTTVTGKVGIGAIPTGFGIEAELT
                     IKAPGVPRDTLQALVDAAHIVCPYSNATRGNIDVTLVVAD"
     gene            2369..3013
                     /locus_tag="PK399_09315"
     CDS             2369..3013
                     /locus_tag="PK399_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPW13356.1"
                     /translation="MSERKPRVLLVHGLLNADWWLRPLAARLRAEGFETALFGYSSVL
                     DGPERAVPKLLERLRREPADALVGHSLGGLIALESLRQAPELAVSRVVCLGSPLCGSQ
                     TARNLAASPWTRPLLGRSAPMLAGGLAGWDGPAQVGVVAGDVAHGLGRLFARFDGGSD
                     GTVGLEETRLPGLADHCTVHSSHTGLVFSPAAVRQAAHFLRHGRFEHAAEPPAV"
     gene            3046..3768
                     /locus_tag="PK399_09320"
     CDS             3046..3768
                     /locus_tag="PK399_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW13357.1"
                     /translation="MGRGPSIEGRKNAVDAKRGKIFTKVIREISVAARAGGGDPAGNP
                     RLRVAIDKGLAVNMSKDVIERAVKKATGELEGVEYEEIRYEGYAPGGVAVIVDCLTDN
                     RVRTVADVRHAFGKFGGNMGTEGSVAFMFRKLGVLSYDTGADEDAITEVAIEAGADDV
                     TVYDDGAIDVITAPDAFAEVKAAMEAAGHVPGFAETTLRADNDIAVEGDTAEQVKKLL
                     AWLEDMDDVQNVYSNADLGDAA"
     gene            3797..4318
                     /gene="ruvC"
                     /locus_tag="PK399_09325"
     CDS             3797..4318
                     /gene="ruvC"
                     /locus_tag="PK399_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPW13358.1"
                     /translation="MTRILGIDPGSQRTGVGIIDVDAAGKVVHVHHQPIKLLDAESFP
                     LRLRLLLDGLWALVEQFAPDEVAIEQVFLSNNAMSALKLGQARGAAIAACVARDLPVS
                     EYAAKEVKLGLVGSGGADKTQVQHMVGIMLGLQGKLQADAADALAVAITHAHVRASAN
                     RLGVATRTAWRKR"
     gene            4461..5048
                     /gene="ruvA"
                     /locus_tag="PK399_09330"
     CDS             4461..5048
                     /gene="ruvA"
                     /locus_tag="PK399_09330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011258432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPW13359.1"
                     /translation="MIGRLKGILIHKAPPWLVVDVQGVGYELEAPMSTFYDLPEVGRE
                     VFLFVHHAQKEDSVSLYGFLRESERRLFRDVQKVSGIGAKIALAVLSGVSVDEFARLL
                     QASDVSALTRIPGIGKKTAERMVVELRDRAAGMATGLPSGVAALPNDPLSEAITALQS
                     LGYKPADADRMARKAAADGDDAATIIRKALQSALR"
     gene            5063..6979
                     /locus_tag="PK399_09335"
     CDS             5063..6979
                     /locus_tag="PK399_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HPW13360.1"
                     /translation="MASNAPHSGPGGHSSRLGLATLVVAAVGVVFGDIGTSPLYTIKE
                     MFNPHFGLVPDARTVKGLLSLGFWSLIMVVTLKYVLVIMRADNEGEGGIMALTALAQR
                     SLKSGSRLSYLVGILGIFGASLFFGDGMITPPITVLGAVEGLAVISPRLANWVVPISL
                     LILFGLFAGQRFGTAKVGKVFGPVMVTWFIVLAALGIYNILDNPIVLRALSPMWAIHF
                     FAEHGWHAVLVLGAVVLTVTGGEALYADMGHFGAKPIRWGWFGFVLPALVLNYFGQGA
                     VLLDDPRAVANPFYMSAPDWAQVPLLVLATTAAAIASQAVITGAFSVTRQAIQLGYLP
                     RLQIRHTSRDTIGQIYVPSINWMLFAAVIVLVLAFRSSTNLATAYGLSVTGTMLIDTL
                     LLAVVAYTRWPDARKWVLPLCALFLVIDLAFLIANGEKLLTGIGAWVPLFIGIAAFTM
                     MRTWRRGRELLREEIRKDGIQLDTFLPGLMLAPPVRVPGTAVFLTADPTVVPHALMHN
                     LKHNKVLHEHNVFLTVDTLNVPYAPKDNRLKIESIAGEDFYRVLVRYGFMETPDVPLA
                     LMRSCDQCDLVFNPMDTTYFASRETIVAGHHRGMPIWRDKLFALMHRNAAPATGFFRI
                     PGNRLVELGAQVEI"
     gene            7037..>7774
                     /locus_tag="PK399_09340"
     CDS             7037..>7774
                     /locus_tag="PK399_09340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPW13361.1"
                     /translation="MPTIPFSLLDLAPVCEGSTPSQAFANALDLARHAEALGYKRYWL
                     AEHHNMPGIASAATSILIGHVAGGTSRIRVGAGGVMLPNHAPLQVAEQFGTLAALYPG
                     RIDLGMGRAPGTDHATARALRRYYDNADAFPRDVQEVLHYFEPVQSGQAVQAVPGSGQ
                     DVPVWILGSSLFGARLAAALGLPYAFASHFAPAAMDEALALYHRDFRPSARLRTPYAM
                     LALNVFGADTRAEAKRLIHVSSFAARQP"
BASE COUNT         1199 a         2588 c         2697 g         1290 t
ORIGIN      
        1 tgatggcggg cttcgaccgc tactaccaga tcgcgcgctg cttccgcgac gaggccctgc
       61 gcgccgatcg ccagctggaa ttcacccagc tcgacatgga gttcgcctgg gtcggcgagc
      121 gtgacgtgca ggacctgacc gaggacatga tccgccacgt gttccgcgag gtgatgggcg
      181 tcgaactggc cgccgcattc ccgcgcatga cctatgccga ggcgatgcgc cgctacggtt
      241 ccgacaagcc ggacctgcgc atcccgctgg agttcgtgga cgtggccgaa caggtgaagg
      301 gctgcgagtt cgcagtgttc acctccgcgg cgaacgatcc cgacggtcgc gtggtcgcgc
      361 tgcgcattcc cggtggtgcg tcgctgtcgc gcaagcagat cgacgactac gccgcgcacg
      421 cggccaagtt cggcgccaag ggcctggcct acgccaagat cgacgaggcc ggtgcggtca
      481 attcgccggt ggccaagttc tttgccgagg gtgcgttcga cgcactgctc cggcacgtcg
      541 gtgccggcaa cggcgacatc gtgttcttcg gtgccggtgc ctacaacaag accagcgatt
      601 tcatgggcgc ggtgcggctg aaggccggca aggatttcaa ccttgtcgcg gatggctggg
      661 cacccctgtg ggtcaccgat ttcccgatgt tcgagtggga cgacgaagaa cagcgttacg
      721 tcgccctgca tcatccgttc accgcgccgg cggtcgattc catcgatgac ctgcgcgcga
      781 atgcgaagac cgcggtgtcg cgtggctacg acatggtgct caacggcaac gagatcggcg
      841 gcggttcgat ccgcatccac aactcggcga tgcagtcggc ggtgttcgag ctgctcggca
      901 tcggcgcgga agaggcggaa ggcaagttcg gcttcctgct ggacgcgctg aagtacgggg
      961 ccccgccgca cggcggcatc gccttcggca tcgaccgcat cgccgcgctg atggccggca
     1021 gcgattcgat ccgcgacgtc atcgccttcc ccaagaccac caccgcgcag tgcctgatga
     1081 ccggggcccc gagcgaagtg ccggacaagc aactggccga ggtgcatgtg tcggtgcggg
     1141 cgcgggagtg atggccgctg ccgaggtgct gctacccgac gcgtgcgaac atcctgttcg
     1201 acgcgtcgcc gcaggccatc catggcctgc ttttcgcagc accccgacaa cgtccatcgg
     1261 gtgagtgaca tgaaagaagc aaccatccgc cgcgccacct ccgatgacgc gcctgccctc
     1321 gccgaactcg gcgcgacgac cttcatcgag gccttcggcc agctgtattc gccggaggac
     1381 ctgcaggcct tcctcgagga aagtcattcg cttgccgcct acgcgaaggt gctggcgaat
     1441 cccgactacg cgctgtggat cgcggagaag gacgggcgcg cgatcggcta tgcgcaggca
     1501 ggcccctgcg ggctgccgca cgacgacgtg cggccgggcg acggcgagct gaagcgcctg
     1561 tacctgctga ggggcgagca gaacggcggc gtcggccgcg ccctgttcga acaggcgctg
     1621 gcctggctgg agcgagatgg cccgcgcacg ctatggatca gcgtgtggtc ggagaactac
     1681 ggcgcgcagc gcttctatgg gcgccacggg ttcgagttcg ccggcgagta cgcattcatc
     1741 gtcggcgagc agcgcgaccg cgaattcatg taccgacggg atccccgcaa cgggtgaagc
     1801 ggacacgaaa acggccaccg cgaagtggcc gttttcgcat gcggcgtggc ggcgcggatc
     1861 agtcggcgac gaccagggtc acgtcgatgt tgccgcgggt ggcattggag tacgggcaga
     1921 cgatgtgggc ggcatcgacc agcgcctgca gggtgtcgcg cggcacgccc ggcgccttga
     1981 tggtgagttc ggcctcgatg ccgaaaccgg tcgggatggc gccgatgccg accttgccgg
     2041 tgaccgtggt gtcggcgggc agggcgacct tctgcttgcc ggccacgtgc ttgagcgcgc
     2101 cgaggaagca ggcggaatag ccggcggcga acaactgctc ggggttggtg cccgggttgc
     2161 cggcgccgcc caggcccttc ggcacggtga gggcgacatc gagcacgccg tcggaggaca
     2221 cggcgcggcc ttcgcggccc ccggtggctg tggcggtggc ggaatacagg acggtttcga
     2281 tcgacatggc ggggttcctg ggggcgggtc ggatgccaag cgtactatcc gcgccatcaa
     2341 cgaggtgcag gcatgcggga aggccccagt gagtgaacgc aagccgcggg tgctgctggt
     2401 gcatggcctg ctcaatgccg actggtggtt gcggccgctg gccgcgcggc tgcgtgccga
     2461 gggcttcgag accgcgctgt tcggctactc cagcgtgctg gacggcccgg aacgggcggt
     2521 gccgaagttg ctggagcggc tgcgccgcga gccggccgac gccttggtcg gccacagcct
     2581 gggcgggctg atcgcgctgg aatcgttgcg gcaggcgccg gagctggcgg tgtcgcgggt
     2641 ggtctgcctg ggctcgccgc tgtgcggcag ccagactgcg cgcaacctcg cggccagtcc
     2701 gtggacgcgt ccgttgctcg gccgcagcgc gccgatgctg gccggcgggc tggccggttg
     2761 ggacgggccg gcgcaggtcg gcgtggtggc cggcgacgtg gcgcatggct tgggccggct
     2821 gttcgcgcgc ttcgatgggg gttcggacgg caccgtgggg ctggaggaaa cccgcctgcc
     2881 cgggctggcc gaccactgca ccgtgcacag cagccacacc gggctggtgt tctcgcccgc
     2941 ggcagtgcgc caggccgcgc atttcctccg ccacggccgc ttcgaacacg ccgccgaacc
     3001 acccgccgta taatccgcgc cctctggttt ccgctggatg ctgcaatggg acgtggtcct
     3061 tccatcgaag gtcgcaagaa cgcggtcgac gccaagcgcg gcaagatctt caccaaggtg
     3121 atccgcgaga tctcggtggc ggcgcgcgcc ggtggcggcg acccggccgg caacccgcgc
     3181 ctgcgggtgg cgatcgacaa gggcctggcg gtcaacatgt ccaaggacgt gatcgagcgt
     3241 gcggtcaaga aggccaccgg cgagctggaa ggcgtcgagt acgaggaaat ccgctacgag
     3301 ggctacgcgc ccggtggcgt ggcggtgatc gtggactgcc tgaccgacaa ccgcgtgcgc
     3361 accgtggccg acgtccgcca tgcgttcggc aagttcggcg gcaacatggg caccgaaggc
     3421 tcggtcgcct tcatgttcag gaagctgggc gtgctcagct acgacaccgg tgccgatgaa
     3481 gacgccatca ccgaggtcgc gatcgaggcg ggtgccgacg acgtgaccgt ctacgacgac
     3541 ggcgccatcg acgtgatcac cgcgcccgat gccttcgccg aggtcaaggc ggcgatggaa
     3601 gccgccggcc atgtccccgg cttcgccgaa accaccctgc gcgccgacaa cgacatcgcg
     3661 gtggaaggcg acaccgccga gcaggtgaag aagctgctgg cgtggctgga agacatggac
     3721 gacgtgcaga acgtctattc caacgccgac ctcggcgacg ctgcgtaaga cacacgccga
     3781 tcccgcgaag cccgacgtga cccgcatcct cggcatcgac cccggttcac agcggaccgg
     3841 cgtgggcatc atcgacgtgg atgccgcggg caaggtcgtg cacgtgcacc accagccgat
     3901 caagttgctg gatgccgagt ccttcccgtt gcgcctgcgc ctgttgctgg atggcctgtg
     3961 ggcgctggtc gaacagttcg cgccggacga ggtggcgatc gaacaggtct tcctgtccaa
     4021 caacgcgatg tccgcgctca agctggggca ggcgcgcggc gcggcgatcg ccgcctgcgt
     4081 ggcccgcgac ctgccggtga gcgaatacgc cgccaaggaa gtgaagctgg ggctggtcgg
     4141 cagcggcggc gccgacaaga cccaggtgca gcacatggtc gggatcatgc tgggcctgca
     4201 gggcaagctg caggccgacg ccgcggacgc gctggcggtg gcgatcaccc atgcgcacgt
     4261 gcgcgccagc gccaaccggc tgggcgtggc cacgcgcacg gcgtggagga aacgatgatg
     4321 cgatgcgtcg cttttgctcg tcattcctgc gcaggccggc aatgctctat ttcggcgaca
     4381 gccggatatc cagtgtcttt catcaaaagt cagagtcgct ggatccctgc cttcgcaggg
     4441 atgaggatgg agagttcgtc atgatcggcc gattgaaagg catcctcatc cacaaggcac
     4501 cgccgtggct ggtggtcgac gtgcaaggcg ttggctacga acttgaagcc ccgatgagca
     4561 ccttctacga cttgccggag gtgggccgcg aggtgttcct gttcgtgcac cacgcacaga
     4621 aggaagacag cgtctcgctg tacggcttcc tgcgcgaatc cgaacgccgc ctgttccgcg
     4681 acgtgcagaa ggtctccggg atcggcgcga agatcgcgct ggcggtcctc tccggcgtgt
     4741 cggtcgatga attcgcgcga ctgctgcagg ccagcgacgt cagcgcgctg acccgcatcc
     4801 ccggcatcgg caagaagacc gccgaacgca tggtggtcga attgcgcgac cgcgccgccg
     4861 gcatggcgac ggggctccca tccggcgtgg cggcgttgcc gaacgatcca ctgtccgaag
     4921 ccatcaccgc cctgcagtcg ctgggctaca agccggccga cgccgaccgc atggcccgca
     4981 aggccgccgc ggacggtgac gatgccgcca ccatcatccg caaggcgcta cagtccgcgc
     5041 tgcgatgagc cacggaaccc acatggcttc gaatgctccc cattccggcc cggggggcca
     5101 ctcttcccgg ctgggcctgg ccacgttggt cgtggccgcg gtcggcgtgg tcttcggcga
     5161 catcggcacc agcccgctgt acacgatcaa ggaaatgttc aacccgcatt tcgggctggt
     5221 cccggatgcg cgcacggtca agggcctgct ctcgctgggc ttctggtcgc tgatcatggt
     5281 ggtgaccctg aagtacgtgc tggtgatcat gcgcgccgac aacgaaggcg agggcggcat
     5341 catggcgctc accgcgctgg cccagcgcag cctgaagtcg ggttcgcggc tcagttacct
     5401 ggtcggcatc ctgggcatct tcggcgcctc gctgttcttc ggcgacggca tgatcacccc
     5461 gccgatcacc gtgctcggcg cggtcgaggg cctggcggtg atctcgccgc gactggcgaa
     5521 ctgggtggtg ccgatcagcc tgctgatcct gttcggcctg ttcgccggcc agcgcttcgg
     5581 caccgcgaag gtgggcaagg tgttcggccc ggtgatggtg acctggttca tcgtgctggc
     5641 ggcgctgggc atctacaaca tcctggacaa cccgatcgtc ctgcgtgcgc tcagcccgat
     5701 gtgggcgatc cacttcttcg ccgagcatgg ctggcatgcg gtgctggtgc ttggcgcggt
     5761 ggtcctcacc gtcaccggcg gcgaggcgct ctacgccgac atgggccatt tcggcgccaa
     5821 gccgatccgc tggggctggt tcggcttcgt gttgcctgcg ctggtgctga actacttcgg
     5881 ccagggcgcc gtcctgctgg acgacccgcg cgcggtggcc aacccgttct acatgtcggc
     5941 gcccgactgg gcgcaggtgc cgttgctggt gctggccacc accgccgcgg cgatcgcgtc
     6001 gcaggcggtg atcactggcg cgttctcggt cacccgccag gcgatccagc tgggctacct
     6061 gccgcgcctg cagatccgcc acaccagccg cgacaccatc ggccagatct acgtgccgtc
     6121 gatcaactgg atgctgttcg ccgcggtcat cgtgctggtg cttgcgttcc gcagttcgac
     6181 caacctggcg acggcctatg gcctgtcggt gaccgggacg atgctgatcg acaccctgtt
     6241 gctggcggtg gtcgcgtata cgcgctggcc ggacgcgcgg aagtgggtgt tgccgctgtg
     6301 cgcgttgttc ctggtgatcg acctggcctt cctgatcgcc aacggcgaga agctgctcac
     6361 cgggatcggt gcgtgggtgc cgctgttcat cggcatcgcc gcattcacga tgatgcgcac
     6421 ctggcggcgt ggccgtgaac tcctgcgcga ggaaatccgc aaggacggca tccagctcga
     6481 taccttcctg cccggcctga tgctggctcc gccggtgcgg gtgccgggca ccgcggtgtt
     6541 cctcaccgcc gaccccaccg tggtgccgca tgcactgatg cacaacctga agcacaacaa
     6601 ggtgttgcac gagcacaacg tgttcctgac cgtcgacacg ctgaatgtcc cgtatgcgcc
     6661 caaggacaat cgattgaaga tcgaatcgat cgccggcgaa gacttctatc gcgtgctggt
     6721 ccgctacggc ttcatggaga cgccggacgt gccgctggcg ctgatgcgca gctgcgacca
     6781 gtgcgacctg gtcttcaatc cgatggacac cacgtatttc gccagccgcg agaccatcgt
     6841 cgccggccac catcgcggca tgccgatctg gcgcgacaag ctgttcgccc tgatgcatcg
     6901 caatgccgcg ccggccaccg gcttcttccg cattcccggc aaccgcctgg tcgaactggg
     6961 cgcgcaagtc gagatctgac ggcgcgcggg cgccgcacgg gttgattccg acccgcagcg
     7021 gctgcatctc atggggatgc cgaccatccc gttctccctg ctcgacctcg ccccggtctg
     7081 cgaaggcagc acgccctcgc aagccttcgc caatgcgctc gacctcgccc gccatgccga
     7141 agcgctgggc tacaagcgct actggctggc cgagcaccac aacatgccgg gcatcgccag
     7201 cgcggcgacc tcgatcctga tcggccatgt cgccggcggt acctcgcgca tccgtgttgg
     7261 tgcgggcggg gtgatgctgc ccaaccacgc gccgctgcag gtggccgagc aattcggcac
     7321 gctggccgcg ttgtatcccg ggcgcatcga cctgggcatg ggccgcgcgc cgggcaccga
     7381 ccacgccacc gccagggcat tgcgccgcta ctacgacaat gccgacgcct tcccgcggga
     7441 tgtccaggaa gtcctgcact acttcgagcc ggtgcaatcg gggcaggccg tgcaggcggt
     7501 tccgggcagc ggccaggacg tgccggtgtg gatcctcggc tccagcctgt tcggcgcgcg
     7561 cctggcggcc gcgctggggc tgccgtatgc cttcgcctcg catttcgccc cggcggcgat
     7621 ggacgaagcg ctcgccctct accaccgcga cttccggccg tcggcgcggc tgcgcacgcc
     7681 ttacgcgatg ctcgcgttga acgtgttcgg tgccgatacc cgcgccgagg cgaagcgcct
     7741 gatccatgta tcgtccttcg cggcacgcca gccg
//
LOCUS       DAOOCX010000101         7751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_8891_length_7751_cov_8.075364, whole genome shotgun sequence.
ACCESSION   DAOOCX010000101 DAOOCX010000000
VERSION     DAOOCX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8891_length_7751_cov_8.075364"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PK399_09345"
     CDS             <1..867
                     /locus_tag="PK399_09345"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPW13362.1"
                     /translation="EETVRELIAHGHTYEEGGALWLRTTDFGDDKDRVMRKSDGTYTY
                     FVPDVAYHLSKWQRGYERAVTELGADHHGSLARVKAGLQALDCGIPAGYPDYVLHQMV
                     TVMRGGEEVKLSKRAGSYLTLRDLIEETSADAVRWFLVARKPDSQLTFDIDLARSQSN
                     DNPVYYVQYAHARVCSLLRQAGEKGYAYDAANGHASLHLLDDAPAQDLMRELSRFPEV
                     VEAAGTTLEPHLVAQYLRELANAFHGWYHASPVLVEDGALRDARLALALATRQVLANG
                     LDLLGVSAPEKM"
     gene            883..1650
                     /locus_tag="PK399_09350"
     CDS             883..1650
                     /locus_tag="PK399_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPW13363.1"
                     /translation="MAARRGKSQARRNGSNGLPGWAWLVIGVLVTLAVVLAVPRILKS
                     EGGADGFFRPKPNPDAQPAATTEDGEAIAPDTATDTTKPDADKPKQTQYDFYTLLPGE
                     GVQMTDAELAASAKAEADAQAKAAQPAATAGTTPGADGSLPTPIAETPSTTPATSAAT
                     AASTAATTGTPAAAAGTDDTRYLLQAGAFGASGDAETVKAKIAMLGLNARVESAQIGG
                     KTVYRVRMGPYGSASELADAKAKLSGGGLPAMAVKTN"
     gene            1650..2471
                     /locus_tag="PK399_09355"
     CDS             1650..2471
                     /locus_tag="PK399_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPW13364.1"
                     /translation="MRFVVLACVALLAGCAGNAGETLVRGTPPLDRSPAAAISADDFV
                     AGSFTARDGTTLPYRLLTPAHIEPGRRYPLIVQFHGSGAIGSDNRAQVERDFAARAWA
                     IPAIRARHPAFVLVPQFPVRSANYDDPMVPRSAVAAPALGAALELVDAVIARQPVDRT
                     RLYASGFSMGGSATWLSLLARPDLFAAAVPISGIAPDRAVADQLTHVPILVLHGDADS
                     ENPIDADRAMVAAIRAAGGRQVRMRRYVGLAHMPPGDLVPGDRWRDWLFAQHRDP"
     gene            2515..2895
                     /locus_tag="PK399_09360"
     CDS             2515..2895
                     /locus_tag="PK399_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13365.1"
                     /translation="MAIETALIKPVVDALMALLQRGEKATLKRNAEAALRDAIRELLL
                     ANPDENKAQARIAVAKAAGILSEDVLLAEDMLEKHRASKARTSGKASSGRKRKAAPAK
                     SDDKLAKPAIARKTVKKPTDQSGT"
     gene            2897..3466
                     /locus_tag="PK399_09365"
     CDS             2897..3466
                     /locus_tag="PK399_09365"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPW13366.1"
                     /translation="MNPITVFYVACAAWAASELWLGWTHRSADRSRQRDAGTLRLLIL
                     VIYACIGVGVWLSFLRPWPIPEPPRTMLFTLGLALMIAGLLFRWWAIRVLAQYFTVDV
                     AIRDDHRIVRDGPYRWLRHPSYTGALATFYGFALCLGDLLALLVVVVPVTLAFLRRIR
                     IEETVLATAFPADYLAYARETKRLLPGIW"
     gene            3490..4257
                     /locus_tag="PK399_09370"
     CDS             3490..4257
                     /locus_tag="PK399_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW13367.1"
                     /translation="MDAHSPRTALITGATAGFGRAAARRFVAAGWQVIATGRRADRLD
                     ALSAELGEAVHTACFDIRDEAAMRAALEALPALFRGIDLLVNNAGLAQGTKPAQHALL
                     SDWKTMIDTNVTALVTLTHALLPQLIARKGAIINISSVAGVYPYPGGNTYGGSKAFVS
                     HFSQGLRSDLHGTGVRVTTIEPGMAETEFTVVRTHGDQAASDTLYTGANPMTAEDIAE
                     AIFWVANLPAHLNINRLEIMPTSQSVAGFQVHREPRP"
     gene            4254..4751
                     /locus_tag="PK399_09375"
     CDS             4254..4751
                     /locus_tag="PK399_09375"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW13368.1"
                     /translation="MNLVGAMLVAVLIGAVLPLQGLINARLGMHIGGPVVAAFVSFLV
                     GTAMLGAYLVATRTPFLMQGSLKLPAWVWAGGVFGAIYVACFTLLIPRIGAAGMICLA
                     VLGQVTASLLLDQFGILQAPKPVDAVRIIGALLVLAGVVLVVAPWRTPAKPAATAEPP
                     ITASR"
     gene            complement(4759..5883)
                     /locus_tag="PK399_09380"
     CDS             complement(4759..5883)
                     /locus_tag="PK399_09380"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPW13369.1"
                     /translation="MSAGSATPTRLPGLDLLRAIAVLWTMQFHGFIVGGLGEDWHWLE
                     RYGWMGVDLFFVLSGYLIGGQLLRPLARGESPSLRDFYLKRAFRILPAFWVVLAIYLL
                     WPGFREAPGMEPWWKFALFVVNLDIDYASNAAFSHAWSLCVEEHFYLLFPALALLLAR
                     KPSATKFWIACIVVLLGGIALRTGAWLHFDALQPQRAWFVEDIYYPTWNRLDGLLMGV
                     MLATWQAYRPDSWARMAKHANVAALLGVAVLALAFWLFRERTGLLGNSIGWPVLSLAL
                     ALLVFAGAQPHGLLGKRALPGAAWIAAISFSLYLVHKPMYGVVQSRLGEWLEGRGIVA
                     YAVYGVASLLAAALLYYAVEKPFLRLRGRVLSRDRVAPAA"
     gene            complement(5880..>7751)
                     /gene="speA"
                     /locus_tag="PK399_09385"
     CDS             complement(5880..>7751)
                     /gene="speA"
                     /locus_tag="PK399_09385"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HPW13370.1"
                     /translation="PDLARKTYSIPHWSDGYFDVDDKGRIVVRPKGANGPAIPLPDVV
                     DASLAQGGNLPVLVRFPDILGDRLGKLQAAFAQARKDWDYAGGYTAVYPIKVNQHAGV
                     AGTLASHHGEGFGLEAGSKPELMAVLALSRPGGLIVCNGYKDREYIRLALIGRKLGLQ
                     TFIVIEKPSELEIALEEAAALGVKPGLGVRMRLASLGSGKWQNSGGDKAKFGLDPRQL
                     LTLWKSLRDAGMGDCLQLLHFHMGSQISNVRDIAAGMREATRYFVELSRLGANITHVD
                     VGGGLGIDYEGTRSRSYNSVNYGIHHYASSIVQPLAEACQAEGLVPPRIVTECGRAMT
                     AHHAVLVANVSEVEQAPEGRVPEPHGDESQPVRHLRELHEEMTRRPAVEVFHEASQAH
                     AEGLSLYTLGQIDLVQRARIDDLFYAIAHAVKARLTYDEKSHRDLLDELSERLVDKYF
                     VNFSVFESMPDVWAIDQVFPITPIERLDEEPTRRGIIADLTCDSDGKIDTYVENEDLD
                     SSLPLHELRKGESYRLGFFLVGAYQEILGDIHNLFGDTDAIEVRCEGDGHRIVQQRRG
                     DTTDVMLDYVGYKLQDLRDAYNGKVDAAHLSAEESAMLKAALEAGLTAYTYLDDTPQ"
BASE COUNT         1278 a         2743 c         2561 g         1169 t
ORIGIN      
        1 gaggagaccg tgcgcgagct gatcgcccac ggccacacct acgaggaagg cggcgcgctg
       61 tggttgcgca ccaccgattt cggcgacgac aaggaccgcg tgatgcgcaa gtccgacggc
      121 acgtacacgt atttcgtgcc ggacgtggcc tatcacctga gcaaatggca gcgtggctac
      181 gagcgcgcgg tcaccgaact gggcgccgac caccacggtt cgctggcgcg ggtgaaagcc
      241 ggcctgcagg cgctggactg cggcatcccg gccggttacc cggactacgt gctgcaccag
      301 atggtcacgg tgatgcgcgg cggcgaggaa gtgaagctgt ccaagcgcgc cggcagctac
      361 ctgaccctgc gcgacctgat cgaggaaacc agcgccgacg cggtgcgctg gttcctggtc
      421 gcgcgcaagc cggattcgca gctgaccttc gacatcgacc tggcccgttc gcagtccaac
      481 gacaatccgg tctactacgt gcagtacgcg catgcgcgcg tgtgcagcct gctgcgccag
      541 gccggcgaga aaggctatgc gtacgatgcg gcgaacggcc atgcgtcgct gcacctcctc
      601 gatgacgcgc cggcgcagga cctgatgcgc gaactgtcgc gcttcccgga agtggtcgaa
      661 gccgccggca ccacgctgga accgcatctg gtcgcgcaat acctgcgtga actggcgaac
      721 gcattccacg gctggtacca cgcatcgcca gtactggtgg aggatggcgc gctgcgcgac
      781 gcgcgcctgg cattggcact ggcgacgcgg caggtgctgg ccaacggact cgacctgctt
      841 ggcgtcagcg ccccggagaa gatgtaaatg aggatcgggt aaatggcagc acgacgcggc
      901 aagtcgcagg cgcgacgcaa tggatcgaac ggtttgccgg gctgggcctg gctggtgatc
      961 ggcgtgctgg tcacgctggc agtggtgctg gcggtgccgc gcatcctcaa gtccgaaggc
     1021 ggtgccgacg gcttcttccg gccgaagccc aatccggatg cgcagccggc agcgaccacc
     1081 gaagacggcg aggcgatcgc ccccgatacc gcgaccgata ccaccaagcc cgacgccgac
     1141 aagccgaagc agacccagta cgacttctac acgctgctcc ccggcgaagg cgtgcagatg
     1201 acggatgccg agctggccgc cagcgccaag gcggaagccg acgcacaggc caaggccgca
     1261 caaccggcgg cgacggctgg caccacgccc ggtgccgatg gcagccttcc gacgccgatc
     1321 gcggagacgc cgtccaccac gccggcgacc tcggcggcaa cggcggcatc gaccgcggcc
     1381 acgacaggca cgccagcagc ggcggccggt acggacgaca cccgctacct gctgcaggct
     1441 ggcgcgttcg gcgcgtccgg cgacgcggaa acggtcaagg cgaagatcgc gatgctcggc
     1501 ctgaacgcgc gcgtcgaatc cgcgcagatc ggcggcaaga cggtctatcg cgtgcgcatg
     1561 ggaccttacg gcagcgcgtc ggaactggcc gatgccaagg ccaagctgtc cggcggcggc
     1621 ctgccggcga tggcggtcaa gaccaactga tgcgcttcgt cgtgctcgcc tgcgtggccc
     1681 tgctggcggg ctgcgccggc aacgcgggcg agaccctggt gcgcggcacc ccgccgctgg
     1741 accgcagtcc ggcggcggcc atctcggcgg acgatttcgt tgcaggcagc ttcaccgcac
     1801 gcgacggcac cacgctgccg tatcgcctgc tcacacccgc gcacatcgaa cccggtcggc
     1861 gctatccgct gatcgtgcag ttccacgggt ccggcgccat cggcagcgac aaccgcgcgc
     1921 aggtcgaacg cgatttcgcc gcgcgcgcct gggcgattcc cgccatccgc gcgcgccatc
     1981 cggccttcgt gctggtgccg cagttccccg ttcgcagcgc gaattacgac gatccgatgg
     2041 tgccccgcag cgccgtcgcg gcgcccgcat tgggggccgc gctggaactg gtcgatgcgg
     2101 tcatcgcgcg acaaccggtg gatcggacgc gcctctacgc aagcggcttc tcgatgggcg
     2161 gatcggccac gtggctgtcg ctgctggcac ggccggacct gttcgccgcg gcggtgccga
     2221 tcagcggcat cgcccccgat cgcgccgtgg ccgatcaact gacgcacgtg ccgatcctgg
     2281 tcctgcatgg cgatgccgat agcgaaaatc ccatcgatgc ggatcgcgcg atggtggccg
     2341 ccatccgtgc cgccggcggc cggcaggtgc ggatgcgccg ctatgtcggc ctggcgcaca
     2401 tgccaccggg cgatctcgtc cccggcgacc ggtggcgcga ctggctgttc gcgcagcatc
     2461 gcgatccctg agacactccg gccgcacagt gccggccccg gaaggatgcg tgccatggcc
     2521 atcgaaaccg cgttgatcaa acccgtcgtc gatgcgctga tggcgctgct ccagcgcggc
     2581 gagaaagcca ccctgaagcg caatgccgaa gccgcgctgc gcgacgccat ccgtgaactg
     2641 ctgctggcca atcccgacga gaacaaggcg caggcgcgga tcgcggtggc caaggcggcc
     2701 ggcatcctct ccgaggacgt gctgctggcc gaggacatgc tggaaaagca tcgcgccagc
     2761 aaggccagga ccagcggcaa ggcaagctcc gggcgcaagc gcaaggccgc gccggccaag
     2821 tccgacgaca agctggccaa gcccgcgatc gcacgcaaga ccgtcaaaaa accgaccgat
     2881 caatccggca catgacatga acccgatcac cgtgttttac gtcgcctgcg ccgcctgggc
     2941 cgccagcgaa ctctggctcg gctggacgca ccgctccgcg gaccgcagcc gccagcgcga
     3001 tgccggcacc ctgcgcctgc tcatcctggt catctacgcc tgcatcggcg tcggcgtgtg
     3061 gctctcgttc ctgcgcccgt ggccgatccc ggaaccgccg cggaccatgc tgttcacgct
     3121 gggcctggcg ctgatgatcg ccggcctgct gttccgctgg tgggcgatcc gggtgctggc
     3181 gcagtacttc accgtggacg tcgcgatccg cgacgaccat cgcatcgtcc gcgacggccc
     3241 ctatcgatgg ctccggcatc cctcgtacac cggcgcattg gccacgttct acggcttcgc
     3301 cctgtgcctg ggcgacctcc tcgcgctgct ggtcgtggtg gtgccggtga ccctggcgtt
     3361 cctgcgccgg atacgtatcg aggaaacggt actggccacg gccttcccgg ccgactacct
     3421 ggcctacgca cgcgagacca agcgcctgtt gccgggcatc tggtgagcgc cgccgcctag
     3481 aatggcggca tggacgcaca ctccccacgc accgccctca tcaccggcgc caccgccggg
     3541 tttggccgcg ccgccgcgcg ccgcttcgtc gccgccggct ggcaggtcat cgccaccggc
     3601 cgccgcgccg accgcctcga cgcactaagc gccgaactcg gcgaggccgt gcacaccgcc
     3661 tgcttcgaca tccgcgatga agccgcgatg cgcgccgcgc tggaggcatt gccggcgctc
     3721 ttccgcggca tcgacctgct ggtcaacaac gcgggcctgg cgcagggcac caagccggcg
     3781 cagcacgcac tgctgtccga ctggaagacc atgatcgaca ccaatgtcac cgcgctggtg
     3841 acgctgaccc atgcgctgct gccgcagctg atcgcgcgca agggcgcgat catcaacatc
     3901 agctcggtcg ccggcgtcta tccgtacccg ggcggcaaca cttatggcgg cagcaaggcc
     3961 ttcgtcagcc atttcagcca gggcctgcgc tccgacctgc acggcaccgg cgtgcgcgtg
     4021 accaccatcg aacccggcat ggccgaaacc gaattcaccg tggtccgcac ccatggcgac
     4081 caggccgcgt cggacacgct gtacaccggt gccaacccga tgaccgccga ggacatcgcc
     4141 gaggcgatct tctgggtcgc caacctgccg gcgcacctca acatcaaccg gctggaaatc
     4201 atgccgacct cgcagtcggt ggcgggcttc caggtccatc gcgagccgcg cccgtgaacc
     4261 tcgtcggcgc gatgctggtg gcggtgctga tcggcgcggt gttgccgctg cagggcctga
     4321 tcaacgcgcg cctgggcatg cacatcggcg ggccggtggt cgcggccttc gtgtcgttcc
     4381 tggtcggcac cgcgatgctc ggtgcctacc tggtcgccac gcgcacgccg ttcctgatgc
     4441 agggcagcct gaaactgccg gcatgggtct gggccggcgg cgtgttcggt gcgatctacg
     4501 tggcctgttt caccttgctg atcccgcgca tcggcgcggc cgggatgatc tgcctggccg
     4561 tcctcggcca ggtgactgcg tcattgctgc tggatcagtt cggcatcctg caggcaccca
     4621 agccggtcga tgcggtgcgc atcattggcg cattgctggt actggcgggc gtggtcctgg
     4681 tggtcgcgcc atggcgaacg ccggccaagc cggccgcgac ggcggagccg ccgatcacgg
     4741 catcccgcta ggcctcgctc aagcggcggg ggcgacgcga tcgcgcgaca acacccgtcc
     4801 gcgcaggcgc aggaacggtt tctccaccgc gtagtacagc agcgccgccg ccagcagcga
     4861 cgccaccccg tacaccgcat acgccacgat gccgcgacct tccagccatt cgccaagccg
     4921 cgactgcacc acgccataca tcggtttgtg caccaggtag aggctgaacg agatcgccgc
     4981 gatccatgcg gcaccgggca gcgcgcgctt gcccagcaat ccatgtggct gcgcgccggc
     5041 gaagaccagc agcgcgagcg ccaacgacag caccggccag ccaatgctgt tgccgagcag
     5101 cccggtgcgt tcgcggaaca gccagaacgc cagcgcgagc accgccacgc cgagcaacgc
     5161 ggcgacattc gcgtgcttcg ccatccgtgc ccatgagtcc ggccgatacg cctgccacgt
     5221 cgccagcatc acgcccatca acaggccatc gaggcgattc cacgtggggt agtagatgtc
     5281 ctcgacgaac cacgcacgct gcggttgcaa cgcgtcgaaa tgcagccacg cgccggtgcg
     5341 cagcgcgatg ccgcccagca ggacgacaat gcacgcgatc cagaacttcg tcgccgacgg
     5401 cttgcgcgcc agcagcaatg cgagcgccgg gaacagcagg tagaaatgct cctccacgca
     5461 cagcgaccac gcgtgcgaga acgccgcgtt gctggcgtag tcgatgtcga ggttgacgac
     5521 gaacagcgcg aacttccacc acggctccat gcccggcgcc tcgcgaaacc ccggccacag
     5581 caggtagatc gccagcacca cccagaacgc cggcaggatc cggaacgcgc gcttgagata
     5641 gaagtcccgc aacgacggcg attcgccgcg cgccagcggc cgcagcagct gcccgccgat
     5701 caggtagccg ctgagcacga agaacagatc cacgcccatc cagccatagc gctccagcca
     5761 gtgccagtcc tcgccgagcc cgccgacgat gaaaccgtgg aactgcatcg tccacagcac
     5821 cgcgatggcg cgcagcaggt ccagccccgg caagcgggtc ggcgtcgccg accccgcgct
     5881 cactgcgggg tgtcgtcgag gtaggtgtac gcggtcaggc ccgcttccag cgccgccttg
     5941 agcatcgcgc tttcctcggc ggacaggtgg gcggcatcga ccttgccgtt gtaggcatcg
     6001 cgcaggtcct gcagcttgta acccacgtag tccagcatca cgtcggtggt atcgccacga
     6061 cgctgctgga cgatgcgatg gccgtcgccc tcgcagcgca cttcgatcgc gtcggtgtcg
     6121 ccgaacaggt tgtggatgtc gcccaggatt tcctggtacg cgccgaccag gaagaagcca
     6181 agccggtagc tctcgccctt gcgcagttcg tgcaacggca gcgaggaatc gaggtcctcg
     6241 ttctcgacat aggtgtcgat cttgccgtcg gagtcgcagg tcaggtcggc gatgatcccg
     6301 cgccgcgtcg gttcctcgtc gaggcgctcg atcggggtga tcgggaacac ctggtcgatc
     6361 gcccacacgt ccggcatgga ttcgaacacg ctgaagttga cgaagtactt gtcgaccagg
     6421 cgttcgctga gctcgtccag gaggtcgcga tggctttttt cgtcgtaggt caggcgcgcc
     6481 ttcaccgcat gcgcgatggc gtagaacagg tcgtcgatgc gcgcgcgctg gaccaggtcg
     6541 atctgcccga gcgtgtacag cgacaggcct tcggcatgcg cctgcgaggc ctcgtggaac
     6601 acttccaccg ccggacgccg ggtcatttcc tcgtgcagtt cgcgcaagtg gcgcaccggc
     6661 tgcgattcat cgccatgcgg ctccggcacg cggccttccg gtgcctgttc gacttccgac
     6721 acgttggcga ccagcaccgc gtggtgcgcg gtcatcgcgc ggccgcattc ggtgacgatc
     6781 cgcggcggca ccagcccttc ggcctggcag gcctcggcca gcggctggac gatgctcgac
     6841 gcgtaatggt ggatgccgta gttgaccgag ttgtagctgc ggctgcgcgt gccttcatag
     6901 tcgatgccga ggccaccgcc cacgtcgacg tgggtaatgt tggcaccgag tcgcgacaac
     6961 tcgacgaaat aacgcgtcgc ctcgcgcata ccggcggcga tgtcgcgcac gttggaaatc
     7021 tggctgccca tgtggaaatg cagcagctgc aggcagtcgc ccatgccggc atcgcgcagc
     7081 gacttccaca gggtgagcaa ctggcgcggg tccagcccga acttggcctt gtcgccgccg
     7141 ctgttctgcc acttgcccga ccccagcgac gccaggcgca tgcgcacgcc caggcccggc
     7201 ttcacgccga gcgcggcggc ttcttccagc gcgatctcca gctcggacgg cttctcgatg
     7261 acgatgaagg tctgcaggcc cagcttgcgg ccgatcagcg ccaagcggat gtattcgcgg
     7321 tccttgtagc cgttgcagac gatcaatccg cccggtcgcg acagcgccag caccgccatc
     7381 agctccggct tgctgccggc ctccaggccg aagccctcgc cgtggtgcga tgccagggtg
     7441 ccggccacgc cggcgtgctg gttgaccttg atcggataca ccgcggtgta gccgccggcg
     7501 tagtcccagt ccttgcgcgc ctgcgcgaac gcggcctgca gcttgcccag gcggtcgccc
     7561 aggatgtccg ggaaacgcac cagcaccggc aggttgccgc cctgcgccag cgaggcatcg
     7621 accacgtcgg gcagggggat ggccgggcca ttcgcgccct tcggccgcac cacgatgcgg
     7681 cccttgtcgt cgacgtcgaa atagccgtcc gaccagtgcg ggatcgagta ggtcttgcgg
     7741 gcaaggtcgg g
//
LOCUS       DAOOCX010000102         7745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_8898_length_7745_cov_9.227178, whole genome shotgun sequence.
ACCESSION   DAOOCX010000102 DAOOCX010000000
VERSION     DAOOCX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8898_length_7745_cov_9.227178"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1285)
                     /gene="nagA"
                     /locus_tag="PK399_09390"
     CDS             complement(155..1285)
                     /gene="nagA"
                     /locus_tag="PK399_09390"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPW13371.1"
                     /translation="MTTHIFHNGHVLTERGFERDACVVVEEGFIVAVLHGEPPTGDLI
                     DLDGGYLVPGFIDAQVNGGGGVLFNNDTSVEAIAAIGRAHRRFGTAGFLPTLISDDAA
                     VMQRAIKATHDAIGQGVPGVLGIHLEGPYLAPARKGTHDDTKFRVPDADEIAMATSLD
                     NGVSLITLAPEQVPPASIRAIVERGGIVAAGHTAGSYDEIRAGIDAGITGFTHLYNAM
                     TPLQGREPGAVGAALEDDDCWCGVIVDGVHVHPASLRVALKAKPRGKVFLVTDAMPMV
                     GADDPSFDLYGETIIAVDGVVRNAAGALAGSALDMASAVRNSVHLLGLPLDEACRMAA
                     QYPAEFLGIGGERGRIAAGYRADLVLLDGEVRVQRSWIGGWE"
     gene            complement(1410..2444)
                     /locus_tag="PK399_09395"
     CDS             complement(1410..2444)
                     /locus_tag="PK399_09395"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPW13372.1"
                     /translation="MHAAADTWMHSEAAEAATAVARQFAANAATIDTLVERLRAHPPR
                     FIVTCARGSSDHAATYGKYLFETTLGLVTASASPSVGSVYAVQPRLDGALFVVISQSG
                     KSPDLLRNAEIAKAGGAHVVALVNVADSPLAKLADTVLPLHAGPETSVAATKSYLCSL
                     AALLQLTARWSGDPQLLAAVDALPAAMRAAWAQDWSPLVSGLRLADHLFVLGRGLGLA
                     GAQEAALKFKETCGLHAEAFSSAEVKHGPMAIVGPDFPVLVFAQDDDIGAGTAAVAAE
                     FVARGAPVWLARPGASGPGVLPLPASPHPACTPILTVQAFYGAANALSIARGHDPDVP
                     PHLKKVTETV"
     gene            complement(2501..3244)
                     /locus_tag="PK399_09400"
     CDS             complement(2501..3244)
                     /locus_tag="PK399_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPW13373.1"
                     /translation="MDSYLLAEFRRQADAHRAPAYQHLRRALQHAIEHGVLGIGQALP
                     PERELAALTGLSRVTVRKAIGGLVADGLLVQRQGSGTFVAERIVKSSSQLTSFSDDLR
                     ARGLDPKSVMLERGIGAATPEEAMALNLSPGMQVARFHRLRTAAGQPLALERTSVPLA
                     VLPDPTLLRDSLYEALAQRGIRPTRALQRLRAIAFDAEQARLLGLAEGSPCLFIERRA
                     FLDDGRVVEFTRSFYRGDAYDFVAELHAP"
     gene            complement(3324..4352)
                     /locus_tag="PK399_09405"
     CDS             complement(3324..4352)
                     /locus_tag="PK399_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW13374.1"
                     /translation="MRKPTIKDVAAQAEVSLKTVSRVVNDESGVHARTRERVQQAIAR
                     LGYLPDQSARSLRTAQSYAIGLLYDNPNPYYVIAMQSGVLSVCRESGYGLQIHPCDSS
                     SKNLANEIVELVQHARLAGIVLAPPMSERQPLVDELVAQGIRLVRIVSAATDPQGGSA
                     CVYVDDRDAAYEITQHLIQLGHARIGFLWGGKSHGSSWERHKGYEEALRDYGITIDPE
                     LVVEGDYSFDDGFRGARRLLSLPQPPTAIFGSNDEIAAGVLAAARSGGLNVPYDLSIA
                     GFEDSPFSKQSWPPLTTAKQATEEIARHAARRLLDEIREQPGHTRNEGFSPELVVRGS
                     TAPPRPKA"
     gene            complement(4458..5747)
                     /locus_tag="PK399_09410"
     CDS             complement(4458..5747)
                     /locus_tag="PK399_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HPW13375.1"
                     /translation="MSTLTSAAPSTTTVRSQFGSIAIIGALFFIFGFVTWLNGPLITF
                     AQLAFELDEVNAFLILTAFFLSYFFLAIPSSWILKRTGMKKGLALGLFTMAVGAALFG
                     EFCTQRWYPGALGGLFVIGGGLALLQTASNPYISILGPIESAAQRIAVMGICNKIAGF
                     LAPIAIGMLVMQGIGELSAQVAAADAATRDRLLDAFAAKIHAPYLAMAGLLALLAIGV
                     LFSPLPEIDPKTANATTASDDAAGDRGILRFPHLWLGALCIFAYVGAEVMAGDAIGAY
                     GKAFGLPLDHTKFFTSFTLGAMLLGYVAGLVAIPRLVSQQVYLTWSALLGVLLVTGAF
                     LTEGYVSVGFVAALGFANAMMWPAIFPLAIQGLGRHTELGSALLVMGIVGGAIIPRLF
                     AGLKQDHDFQLVFLLLMVPCYLYILFFAMRGHRAGRG"
     gene            6062..7141
                     /locus_tag="PK399_09415"
     CDS             6062..7141
                     /locus_tag="PK399_09415"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HPW13376.1"
                     /translation="MGDTAGIGGEADVVALRPHASFVPSPVASASCFVAADVGGTNAR
                     IALVQAEGERIEILDYRRYPCGQYPSLTAILSDFTGRQLDAGQPRAAFERMAIASAGV
                     VLDGEVINSNLPWRISLQDLRDTLGLHELHVINDFAAAAHGTQRLDPAESRLLTPGVS
                     VAEAGPALVVGPGTGLGAAVCIPHPRGTVVLPTEAGMTAFAPGTEREVEILRWMQRHG
                     TRHVSTEQLVSGPGLVNLYQALCELESARALLREPAAISQSALAGDPMALEAVLTFCA
                     LLGSVIGDLAVLSGARAVHVAGGIVPQLVDFLPRSDFRARLVDKGAMRAVLERVPVRL
                     IENERLGALGAASWYLQQRQDRIDP"
     gene            7256..>7745
                     /locus_tag="PK399_09420"
     CDS             7256..>7745
                     /locus_tag="PK399_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005414491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPW13377.1"
                     /translation="MQVRKNMLALSIVAALGVTGTATAQDAPKTDPKDLDTVVVTGIR
                     GSVEKSLDVKRDAKAHVEVITAEDVGKMPDKNVADSLQRLPGVTISSAGASEGGFDEN
                     DRVSMRGTNPSLTLTQINGHSVSSGDWFVLNQSDNAGRSVSYTLLPSSLVSQVVVHKT
                     SQA"
BASE COUNT         1316 a         2557 c         2709 g         1163 t
ORIGIN      
        1 tgatcctgac cggccacccg cctgcgaaaa agaagccgga tcaagccccg tagaccggat
       61 aaggccaaag gccgcatccg ggattgcgtt cgaaagagtc ccggatgcgc tgcgcttatc
      121 cgggctacaa aggcttgggc aacgggtggt gcgtttattc ccagccacca atccagctgc
      181 gctgcacgcg cacctcgcca tccagcaata ccagatccgc gcgatagccc gccgcgatgc
      241 ggccacgttc gccgccaatg ccgaggaact ccgccggata ttgcgcggcc atccggcagg
      301 cttcgtccag cggcaggccg agcaagtgca cgctgttgcg cacggcgctg gccatgtcca
      361 gcgcggaacc ggccagcgct ccggcggcat tgcggaccac gccatcgacc gcgatgatgg
      421 tttcgccgta gagatcgaac gaggggtcat cggcgccgac catcggcatc gcatcggtga
      481 ccaggaacac cttgccgcgc ggcttcgcct tcagcgcgac gcgcaggctg gccggatgga
      541 cgtgcacgcc atcgacgatc acgccgcacc agcagtcgtc gtcttccagc gcggcaccga
      601 ccgcgccggg ctcgcggccc tgcagcgggg tcatcgcgtt gtacagatgg gtgaagccgg
      661 tgatgccggc atcgatgccc gcgcggattt cgtcgtaact gcctgcggtg tggccggcgg
      721 cgacgatgcc gccgcgttcg acgatcgcgc ggatgctcgc gggcggcact tgctccggtg
      781 ccagcgtgat caggctcacg ccgttgtcga gcgatgtcgc catcgcgatc tcgtcggcgt
      841 cgggcacgcg gaacttggtg tcgtcgtggg tgcccttgcg cgcgggtgcc aggtacgggc
      901 cttccaggtg gatgccgagc acgccgggca cgccttgtcc gatcgcgtca tgtgtcgcct
      961 tgatcgcgcg ctgcatgacc gcggcatcgt cgctgatcaa tgtgggcagg aagccggcgg
     1021 tgccgaaccg ccgatgcgcg cggccgatcg cggcgatcgc ctccaccgag gtgtcgttgt
     1081 tgaacagcac gccgccgccg ccgttgacct gggcatcgat gaagccgggc acgagatagc
     1141 cgccatcgag gtcgatgaga tcgccggttg gtggttcgcc atgcagcacg gcgacgatga
     1201 agccttcttc cacgacgacg cacgcgtcgc gctcgaagcc gcgttcggtg aggacgtgtc
     1261 cgttgtggaa gatgtgcgtg gtcatggttt cgttttcttc tccctccctt tcgcgcagcg
     1321 aaggggtggg ttgggggagg ggttgcttgc cggtgaggag cacctcccgg cctcctcctt
     1381 cgcctgcggc gaaaggggag gaaaaaccat cacaccgtct ctgtcacttt cttgaggtgc
     1441 ggcggcacgt cgggatcatg cccgcgcgcg atcgagagcg cattcgccgc gccgtagaac
     1501 gcctgcacgg tgaggatcgg cgtgcaggcc ggatgtggcg aggccggcag cggcagcacg
     1561 ccgggtccgc tggcacccgg tcgcgcgagc cagaccggcg cgccgcgcgc aacgaactcc
     1621 gccgcaactg cagcggtgcc ggcgccgatg tcgtcgtcct gggcgaacac cagcaccggg
     1681 aaatccggac cgacgatcgc catcggcccg tgcttcacct cggcggagga aaacgcctcc
     1741 gcatgcaggc cacaggtttc cttgaacttc agcgcggctt cctgcgcgcc ggccaggccc
     1801 aggccgcgac cgagcacgaa caggtggtcg gccaaccgca agccggacac cagcggcgac
     1861 cagtcctgcg cccacgctgc acgcatcgcc gcgggcagcg catcgactgc ggcgagcagt
     1921 tgggggtcgc cactccagcg tgcggtgagc tgcagcagtg cggcgagtga acacaggtaa
     1981 ctcttggtcg cggccacgct ggtctcggga ccggcatgca gcggcagcac ggtgtcggcg
     2041 agtttggcca gcggcgaatc cgcgacgttg accagcgcca ccacgtgcgc gccgccggcc
     2101 ttggcgatct ccgcattgcg cagcaggtcc gggctcttgc cggattgcga gatcaccacg
     2161 aaaagagcgc cgtcgagtcg gggctgcacc gcgtacaccg agcccaccga cggcgatgcc
     2221 gaggcggtca ccaaccccag cgtggtctcg aacaggtatt tgccgtaggt cgcggcgtgg
     2281 tcggagctgc cgcgcgcgca ggtcacgatg aagcgcggtg gatgcgcgcg caggcgctcg
     2341 accagggtgt cgatggtcgc ggcgttggcg gcgaactggc gggcgacggc ggtcgccgcc
     2401 tctgccgctt cgctgtgcat ccaggtgtcg gcggcggcgt gcatgggcgt ggttccgaaa
     2461 ggtgagtgat cgcgtggtgt cgcgtggtcg gtgtcgaagc tcatggggca tgcaactcgg
     2521 cgacgaagtc gtaggcatcg ccgcggtaga aggaacgggt gaactcgacc acgcggccgt
     2581 cgtcgaggaa ggcgcggcgt tcgatgaaga ggcagggact gccttccgcc aggccgagca
     2641 agcgcgcctg ctcggcatcg aaggcgatcg cacgcagccg ctgcagcgcg cgggtcgggc
     2701 ggatcccgcg ctgggcgagc gcttcgtaca gcgaatcgcg caacagggtc gggtccggca
     2761 gcaccgccag cggcacgcta gtgcgttcca gcgcgagtgg ctggccggca gcggtgcgca
     2821 ggcgatggaa gcgcgcgacc tgcataccgg gcgacaggtt cagcgccatc gcttcctccg
     2881 gcgtggccgc gccgatgccg cgttcgagca tcaccgactt cgggtcgagg ccgcgcgcgc
     2941 gcaggtcgtc gctgaagctg gtcagctgcg aggacgactt gacgatgcgc tcggcgacga
     3001 aggtgccgga accctgccgc tgcaccagca ggccgtcggc gaccagccca ccgatcgcct
     3061 tgcgcacggt gacccgcgac aggcccgtca gcgccgcgag ttcgcgttcc ggtggcagcg
     3121 cctggccaat gccgagcacg ccgtgttcga tcgcgtgctg gagggcgcgg cgcaggtgct
     3181 ggtaggccgg cgcgcgatgc gcatcggcct ggcggcggaa ttcggcaagg aggtagctgt
     3241 ccacgccgaa agaataccaa tcagatacca aaaggcaata tcaggccgaa aaatgacctg
     3301 caacccacga ttggtattga aaatcaggct ttcggccgtg gcggcgcagt cgagccgcgc
     3361 accaccagct ccgggctgaa gccctcgttg cgggtgtgcc cgggctgttc gcggatctcg
     3421 tccagcaggc ggcgcgcggc gtggcgggca atctcttcgg tggcctgctt ggcggtggtc
     3481 agcggtggcc agctctgctt ggaaaacggg ctgtcctcga agccggcgat cgacaggtcg
     3541 tagggcacgt tgagcccgcc ggagcgtgcc gcggcgagca cgccggcggc gatttcgtcg
     3601 ttgctgccga agatcgcggt gggcggttgc ggcagcgaca gcaagcgccg cgcgccgcgg
     3661 aagccgtcgt cgaaggaata atcgccttcg accaccagtt ccgggtcgat ggtgatgccg
     3721 taatcgcgca gtgcttcttc gtaacccttg tggcgttccc acgacgagcc atgcgacttg
     3781 ccgccccaca ggaagccgat gcgcgcgtgg ccgagctgga tgaggtgctg ggtgatctcg
     3841 taggcggcgt cgcggtcgtc cacatagacg caggcgctgc cgccctgcgg atcggtggca
     3901 gcggacacga tccgcaccag gcggatgccc tgcgcgacca gttcgtccac cagcggctgc
     3961 cgctccgaca tcggcggcgc cagcacgatg ccggccaaac gcgcgtgctg gaccagctcg
     4021 acgatttcat tcgcaagatt cttcgatgac gaatcgcagg gatggatctg caggccgtag
     4081 ccggactcgc ggcagaccga cagcacgccg ctctgcatcg cgatgacgta atacgggttc
     4141 gggttgtcgt agagcaggcc gatcgcgtac gactgcgcgg tgcgcaggct gcgcgcggac
     4201 tggtcgggca ggtagcccag gcgggcgatg gcctgctgca cgcgttcgcg ggtgcgggcg
     4261 tggacgccgc tttcgtcgtt gaccacgcgc gaaacggtct tcagcgagac ctcggcctgg
     4321 gcggcgacat ccttgatcgt gggcttgcgc aacgtggcct cgcgtggggg cgccggtcgc
     4381 gtggtcgcgg gcctggcgtg ggaagggcgg cgcatagggt aacggtcgcc ggttaagcaa
     4441 aagttggcgc gtgcgcctca gccgcggccc gcgcgatgcc cgcgcatcgc gaagaacagg
     4501 atgtacaggt agcacggcac catcagcagc aggaacacca gctggaaatc gtggtcctgc
     4561 ttcagcccgg cgaacaggcg cgggatgatc gcgccaccga cgatgcccat caccagcagg
     4621 gcggagccga gttcggtatg ccggcccagg ccctggatcg ccagcgggaa gatcgccggc
     4681 cacatcatcg cgttggcgaa gcccagcgcg gcaacgaagc ccaccgacac atagccctcg
     4741 gtgaggaacg cgccggtcac cagcagcacg cccagcaatg ccgaccacgt gaggtagacc
     4801 tgctgcgaga cgaggcgcgg gatggccacc aggccggcca cgtagcccag caacatcgca
     4861 ccgagggtga acgaggtgaa gaacttggtg tggtccagcg gcaggccgaa ggccttgcca
     4921 taggcgccga tggcatcgcc cgccatcact tcggcgccga cgtaggcaaa gatgcacagc
     4981 gcgccgagcc acaggtgcgg gaaacgcagg atgccgcgat cacccgcggc atcgtccgac
     5041 gcggtggtgg cgttcgcggt cttcggatcg atttccggca gcggcgagaa cagcacgccg
     5101 atcgccagca aggccagcag gcccgccatc gccaggtagg gcgcgtggat cttcgcggcg
     5161 aacgcatcca gcaaccggtc ccgggtggcg gcgtccgcgg cggcgacctg tgcggacagc
     5221 tcgccgatgc cctgcatcac cagcatgccg atggcgatgg gcgcgaggaa gcctgcgatc
     5281 ttgttgcaga tgcccatcac cgcgatgcgc tgcgccgcgc tttcgatcgg gccgaggatg
     5341 ctgatgtagg gattcgacgc ggtctgcagc agcgccagcc cgccgccgat gacaaacagc
     5401 ccgccaagcg cgcccgggta ccagcgctgc gtgcagaact cgccgaacag cgccgcgccg
     5461 accgccatcg tgaacaggcc gagtgccagg cccttcttca tgccggtgcg cttgaggatc
     5521 cacgacgacg ggatcgccag gaagaaatac gagaggaaaa acgcggtgag gatcaggaac
     5581 gcgttgacct cgtcgagttc gaacgccagc tgggcgaagg tgatcagcgg cccgttgagc
     5641 caggtgacga agccgaagat gaagaacagg gcgccgatga tcgcgatgga gccgaactgg
     5701 ctgcgcaccg tggtggtcga cggcgcggcg gacgtaagcg tggacatgcg gtttcccctc
     5761 ccgggaatgg cggtcgtccg atgcatcgag ggcgccgaac gggtctcgat tgacgcatac
     5821 gacaaagcca ggtgcaacgt tgtcggtatt tggcctgttc tgtccaccac ttccttggaa
     5881 aaccgcttca agcgagcatt tcgcaggcgt cggttcgact aatttgcatg ctgcacaaca
     5941 gcaaaaatcg accggttctc agctgaatgg ggcgcaagga aagaaatttc tgtcagattc
     6001 ttgacaacgt tgtctaaacc cccgcacatt cgcctcgccg cactggcatg gggagggcgg
     6061 gatgggcgat acggcaggca tcgggggcga ggcagacgtg gtcgcattgc ggccgcatgc
     6121 gtcgttcgtg ccgtccccgg tcgcgtcggc gtcctgcttc gtggccgccg acgtcggtgg
     6181 caccaatgcg cgcatcgcgc tggtgcaggc cgagggcgag cgcatcgaga tcctcgacta
     6241 ccgccgctat ccctgcggcc agtaccccag cctgaccgcg atcctgtccg atttcaccgg
     6301 ccgccagctg gatgccggcc agccgcgcgc cgccttcgag cgcatggcga tcgccagcgc
     6361 cggcgtggtc ctggatggcg aggtgatcaa ttccaacctg ccgtggcgga tctcgctgca
     6421 ggacctgcgc gacaccctgg gcctgcacga gttgcacgtc atcaacgatt tcgccgcggc
     6481 agcgcacggc acccaacggc tggacccggc ggagtcgcgc ctgctgaccc ccggcgtgtc
     6541 ggtggccgag gccggcccgg ccctggtggt gggcccgggc accggcctgg gcgcggcggt
     6601 gtgtatcccg cacccgcgcg gcaccgtggt gctgccgacc gaagccggga tgaccgcatt
     6661 cgcgcccggc accgagcgcg aagtcgagat cctgcgctgg atgcagcgcc acggcacccg
     6721 ccacgtctcg accgagcaac tggtgtccgg ccccggcctg gtcaacctct accaggcgct
     6781 gtgcgaactg gaatctgcgc gcgccctgct gcgtgagccg gcggccatca gccagtccgc
     6841 gctggccggc gatccgatgg cgctggaggc ggtgctgacc ttctgtgccc tcctgggcag
     6901 cgtgatcggc gatctcgcgg tgctcagtgg cgcgcgcgcg gtccacgtgg ccggcggcat
     6961 cgtcccgcaa ctggtcgact tcctgccgcg cagcgacttc cgcgcgcgcc tggtcgacaa
     7021 gggcgcgatg cgcgccgtgc tggaacgcgt gccggtgcgc ctgatcgaga acgaacggct
     7081 cggcgcgctg ggcgcggcga gctggtacct gcagcaacgg caagaccgca tcgatccgta
     7141 accgaaccac ccacaacaac atggccgcgc atggacgggc cagcaagcaa cgacgaacca
     7201 gccaagggtc cacaggaacc ggacccgacg ccatcactgc cccgggagag gaatcatgca
     7261 agtccgcaag aacatgttgg cgctcagcat cgtcgccgcg cttggcgtta caggcacggc
     7321 cacggcgcag gacgcgccca agacagaccc caaggacctc gacaccgtcg tcgtcaccgg
     7381 catccgcggc agcgtggaga agtcgctcga cgtgaagcgt gatgccaagg cgcacgtgga
     7441 ggtgatcacc gccgaagacg tgggcaagat gcccgacaag aacgtggccg actcgctgca
     7501 gcgcctgccc ggcgtgacca tcagctcggc cggcgccagc gaaggcggct tcgacgagaa
     7561 cgaccgcgtg tcgatgcgcg gcaccaaccc gagcctgacg ctcacccaga tcaacgggca
     7621 ttcggtgtcc tccggcgact ggttcgtgct caaccagagc gacaacgccg gccgcagcgt
     7681 cagctacacc ctgctgccgt ccagcctggt cagccaggtg gtggtgcaca agacctcgca
     7741 ggcca
//
LOCUS       DAOOCX010000103         7649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_9047_length_7649_cov_6.546747, whole genome shotgun sequence.
ACCESSION   DAOOCX010000103 DAOOCX010000000
VERSION     DAOOCX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9047_length_7649_cov_6.546747"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..958)
                     /locus_tag="PK399_09425"
     CDS             complement(77..958)
                     /locus_tag="PK399_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HPW13378.1"
                     /translation="MAHRTKPWRLTIAATIVVTVAYGAWTLSRQPGSPTPRTATSTIA
                     GIPAGAVVVDTRHYRIHSSASPKDATEIGQALEALYTAYGDLFPIANDPGRLPLILYR
                     DRAEFSRNNRSSPWAEAYYLDPACYAYFDAAAPNPHHWAMHEAVHQLARKASGFPRNR
                     WIDEGLASYFSASRRVDGRLQSGNIDPTAYPAWWLADAAGKRDMPIRLEVLLSGSGGP
                     DVNTHFNQYYLSAWSLTHFLLHRHSGRHVGAYRAFLEAGADRNAFLRHIGTIETIESE
                     WIPYFRRQVEASIKASP"
     gene            complement(974..1369)
                     /locus_tag="PK399_09430"
     CDS             complement(974..1369)
                     /locus_tag="PK399_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HPW13379.1"
                     /translation="MSIAYWCILIAAVLPLIWVGFAKSGAPGYNNKDPRGWVAKQDGY
                     RVRNAYNAHLNAFEAFPTFAAGVLMAQFAGVDPSRVNMLAIAFVGFRVLHGASYIAAI
                     ASMRSLVWMGGFACTIGLMVLAALKLGTA"
     gene            complement(1459..3198)
                     /locus_tag="PK399_09435"
     CDS             complement(1459..3198)
                     /locus_tag="PK399_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HPW13380.1"
                     /translation="MRATTLSLALLALCACTPRGDQQAAVDATADAAPATDAAPTSTH
                     AFNDAIDAGDFAEHVKQLASDAFGGRGPGTPGEEKTVEYIKAQFARIGLQPGNGSDWF
                     QTVPMVETTADEASASMVATVGGATQALKFGTDMVIGTRTGQAQVDIKDSPLVFIGYG
                     VDAPEQQWNDYAGIDVKGKTVVMLVNDPGFHAGDAKLFDGKRMTYYGRWTYKFEEAAR
                     KGAAAALIIHDTEGASYGWDVVKNSWSGPQYDLPAKDDPAPRVPAQGWITGEAATALF
                     KAAGQDLAKLRAAANKRGFKPVPLDASLSLALKSTSVEKASRNVVGLLPGSETPDEAI
                     VYMAHWDHLGTHDGETGDNIYNGAVDNATGVAAIMEIAESFRKAKVPPKRSILFLAVT
                     LEESGLLGSKYYVAHPVIPLDKTVAVFNLDALSPVGKARDITVVGKGSSQLEDLLKPL
                     LDAQKRKPVGETNTAAGYYFRSDHFNFAKAGVPSLYLDSGTDLLEGGQAAGDAAGKDY
                     TDNRYHKAGDHFDAATWKLDGIIQDLEVVTTLGTTLANDGQWPNWYAGNPFKAARDAM
                     RPVAPESAPPAGK"
     gene            complement(3248..3925)
                     /locus_tag="PK399_09440"
     CDS             complement(3248..3925)
                     /locus_tag="PK399_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13381.1"
                     /translation="MKRSLLFVALLAFSSVAPAHDHEGPGRDIDKVNGSITASAGETY
                     GDLSTVNGGIDLERGARVRDVETVNGGIEAADDVGAHSVSTVNGAIRFGKQARIDGDV
                     ETVNGGIFIDRGGNVRDDVSTVNGAIGLVDTDLGGGIETVNGDVTVGVGSHVKGGIHV
                     EKPNGNGMQWGKQRIPRIVIGPNAVVDGPLVFERDVVLYVHDSARIGSVRGATAKRYS
                     TATPPQE"
     gene            4037..5581
                     /gene="gpmI"
                     /locus_tag="PK399_09445"
     CDS             4037..5581
                     /gene="gpmI"
                     /locus_tag="PK399_09445"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPW13382.1"
                     /translation="MPANAPVRPKPVMLLILDGWGHREEVEDNALAQADLPNWRHLLA
                     TCPHTLVHTEGRHVGLPDGQMGNSEVGHMNLGAGRIVYQDLTRVDAAIEDGSFFANAE
                     LNAACRDAIAHDGTLHVMGLLSPGGVHSHEAHIFAMLDLAHRAGVPRVAVHAFLDGRD
                     TPPQSAEASLRALQALCDRLGNARIASVGGRYFAMDRDKRWDRVRRGWDAIVEAAGEH
                     RATDALSALQAAYARGENDEFVAPTVIGAASPMRDGDAVVFMNFRADRARQLTAAFVD
                     PGFEGFVARRPALSRFVCLSEYDAKLPAPLAFGPDDLRHTFGEIVADAGLTQLRIAET
                     EKYAHVTFFFSGGREALFAGEERILVPSPQVATYDLQPEMSCPELTDKLVAAIGSGRF
                     DAIVCNIANPDMVGHSGILAAAIAAAQAVDIAIGRIAQAVREVGGALIITADHGNLEM
                     MRDPATGQPHTAHTVGPVPLVYVGDRDVALRGGGALRDVAPTLLDLMNVQPPAEMSGQ
                     SLLLPA"
     gene            5581..6804
                     /locus_tag="PK399_09450"
     CDS             5581..6804
                     /locus_tag="PK399_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPW13383.1"
                     /translation="MRRLRAVALCAVLAMLAGVAHAQDRSDAERRLQGVRNELKQVAA
                     ERRKLEGERGDASRKLREADEQVGGVQRSLQKTQTTLQRDGAELLRLQAERARHVGDL
                     EAKKTELARLLRAAQFAGSEAPLKTMLAQDRVAEAERALTYQGYLQRGQVQRIRVLSA
                     ELQRIDALEREIVARREALDRERRTQASQLTELQQARKQRAGLLAGIDQQYRDRAGRE
                     KALGRDAKALQDLLSRLRAAAAKAARDAARAKAEAERQAVASGKPKPKPSARRATVAS
                     APAMRVGGLGWPVAGNLLASFGGTLPDGRRSDGVLIAAAAGTAVKAMADGTVVYADWM
                     TGYGNILIIDHGNGYMSLYAHNDGLSRDAGDVVKRGDTVASVGTSGGQDVPALYFELR
                     RNGAPVNPASWLTRQ"
     gene            6905..>7649
                     /locus_tag="PK399_09455"
     CDS             6905..>7649
                     /locus_tag="PK399_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPW13384.1"
                     /translation="MRAKPLSLVIAILLGVAAAPSFAQSPASAPAAPAATEPAAAPQG
                     EQDPLASDDPDSAKTDAAKVPLDEIRRYVMVYNAIRQAYVDPVDDHALMQSAVRGLLL
                     DLDPHSAYLKKADAEEFDELTSGAYDGVGLELQQLPEGGVRIVAPLDDGPAERAGLRS
                     GDLIIAIDGKPLEAGAGDASAPLRGKAGTKVVVRIVRKGTAQPFDVTLVRDTIRTTSV
                     RSRMLEPGFGYVRLSSFQAATASDFNKAVD"
BASE COUNT         1179 a         2549 c         2678 g         1243 t
ORIGIN      
        1 gggttttgtc tttttggggc cgtccgcgca agcgggacaa gttaatagtg tccccgctca
       61 tcctgccccg gcatcgtcaa ggcgaggctt tgattgaagc ttcgacttgc cgcctgaaat
      121 atggaatcca ttccgattcg atggtttcta tggtgccgat gtggcggagg aaggcatttc
      181 gatcggcgcc tgcttcgagg aaggcgcgat aggcgccgac atggcggcca ctgtggcgat
      241 gcaggaggaa gtgggtcagg ctccaggcgc tcaggtaata ctggttgaag tgggtattca
      301 cgtcgggccc gccgctgccg gaaagcagta cctcaagccg gatcggcatg tcgcgcttgc
      361 cggccgcatc cgccagccac cacgccggat aggcggtggg atcgatgttg ccggactgca
      421 gtcggccatc gacgcgtcgg cttgcggaga agtaactcgc cagcccttcg tcgatccatc
      481 ggttgcgcgg gaaccccgag gccttgcggg cgagctggtg taccgcttcg tgcatcgccc
      541 agtgatgcgg attgggggcg gcggcatcga aataggcata gcaggcggga tcgaggtaat
      601 aggcctcggc ccaggggctg ctgcggttgt tgcgcgagaa ctcggcccgg tcccggtaca
      661 ggatgagcgg gagtcgcccg ggatcgttgg cgatcggaaa cagatcgcca tatgccgtgt
      721 acaacgcctc gagggcttgg ccgatctcgg tggcatcctt gggtgatgcg ctggaatgga
      781 tccggtaatg ccgggtgtcg acgacgacgg ccccggccgg gatgccggcg atggtcgacg
      841 tcgccgttcg cggagttggc gatccaggtt gcctagacag tgtccatgca ccgtaagcga
      901 cggtgacgac gatggtcgcc gctatcgtca gccgccacgg cttggtgcgg tgcgccacca
      961 gggattggcc gcgtcaggcc gtgccgagct tcagcgccgc caggaccatc aatccgatcg
     1021 tgcaggcgaa gccgcccatc cagaccaggc tgcgcatgga cgcgatcgcg gcaatgtagg
     1081 aggcgccatg gaggacccgg aatcccacga aggcgatggc gagcatgttc acccggctcg
     1141 ggtcgacgcc ggcgaactgc gccatcagca cgcccgcagc gaaggtgggg aacgcctcga
     1201 aggcattgag gtgggcgttg taggcgttgc gaacgcggta gccatcctgc ttggcgaccc
     1261 agccgcgcgg gtccttgttg ttgtagcccg gcgcgccgct cttggcgaag ccgacccaga
     1321 tgagcggcag gacggcggcg atcaggatgc accagtaagc gatggacatg gcggccctcc
     1381 ccgggcacga atggaggccg ccagcctagc cgatcccgct atcgccggtt tccgtggccg
     1441 ccggcgatgg cgaccttcct atttcccggc agggggcgcg ctctccggcg cgacgggacg
     1501 catcgcatcg cgcgcggcct tgaacggatt gcccgcgtac cagttcggcc actggccatc
     1561 gttcgccagc gtggtgccca gcgtggtcac cacctccagg tcctggatga tgccgtccag
     1621 cttccaggtc gcggcatcga agtggtcgcc ggccttgtgg taacggttgt cggtgtaatc
     1681 cttgccggca gcgtcccccg ccgcctggcc gccttccagc aagtcggtgc cactgtcgag
     1741 gtacagcgat ggcacgccgg ccttggcgaa gttgaagtgg tcggagcgga agtagtagcc
     1801 ggccgcggta ttggtttcgc cgacgggctt gcgcttctgc gcatccagca ggggcttcaa
     1861 caggtcttcc agttgcgaac tgcccttgcc gaccacggtg atgtcgcggg ccttgccgac
     1921 cggcgacagt gcgtcgaggt tgaacacggc cacggtcttg tccagcggga tgaccgggtg
     1981 ggcaacgtag tacttggagc cgagcaggcc cgattcctcc agcgtcaccg ccaggaacag
     2041 gatcgagcgc ttcggcggga ccttggcctt gcggaacgac tcggcgattt ccatgatcgc
     2101 ggcgacaccg gtcgcgttgt cgaccgcgcc gttgtagatg ttgtccccgg tctcgccgtc
     2161 gtgcgtgccc agatggtccc agtgcgccat gtagacgatc gcctcgtccg gcgtctcgct
     2221 gccgggcaac aggccgacca cgttgcgcga agccttttcc accgaggtgc tcttcagcgc
     2281 cagcgacagc gaggcgtcca gcggtaccgg cttgaacccg cgcttgttgg cggccgcgcg
     2341 cagcttggcc aggtcctgcc cggcggcctt gaacagggca gtcgcggcct cgccggtgat
     2401 ccagccctgc gctggcacgc gcggtgcggg atcatccttc gctggcaggt cgtactgtgg
     2461 tccggaccag gaattcttga cgacgtccca accgtaggat gcgccttccg tgtcgtggat
     2521 gatcagcgca gcggcggcgc ccttgcgcgc ggcttcctcg aacttgtagg tccagcgccc
     2581 gtagtaggtc attcgcttgc cgtcgaacag cttcgcgtcg cccgcgtgga agccggggtc
     2641 gttgaccagc atcaccacgg tcttgccctt cacgtcgatg cccgcgtagt cgttccattg
     2701 ctgctccggc gcgtccacgc cgtaaccgat gaagaccagc ggactgtcct tgatgtcgac
     2761 ctgggcctgg ccggtacgcg tgccgatcac catgtcggtg ccgaacttca gcgcctgcgt
     2821 cgcaccgccg accgtcgcca ccatcgaggc cgacgcttcg tcggcggtgg tttccaccat
     2881 cggcacggtc tggaaccagt cgctgccgtt gccgggttgc aggccgatgc gcgcgaactg
     2941 cgccttgatg tactcgaccg tcttctcttc gcccggagtg ccggggccgc ggccaccgaa
     3001 cgcgtcggag gccagttgct tcacatgctc ggcgaaatcg ccggcatcga tcgcatcgtt
     3061 gaatgcatgg gtgctggtgg gtgcggcatc ggtggccggt gcggcgtcgg cggtggcatc
     3121 gacggcggct tgctggtcgc cgcgtggcgt gcaggcgcac agcgccagca gggcgaggga
     3181 aagcgtggtg gcgcgcatcg cgggactcct tgtcggatgt cccgcgattc tagccgcgtc
     3241 gcggcggtca ttcctgcggc ggggtcgccg tgctgtaacg cttcgcggtg gcgccgcgca
     3301 cgctgccgat gcgggcgctg tcgtgcacgt acaggaccac gtcgcgctcg aacaccagcg
     3361 ggccgtcgac cacggcgttc gggccgatga cgatgcgcgg gatccgctgc ttgccccact
     3421 gcatgccgtt gccgttcggc ttctcgacgt ggatgccgcc cttcacgtgc gagcccacgc
     3481 cgacggtgac atcgccattc acggtttcga tgccgccacc caggtcggtg tccaccaggc
     3541 cgatcgcgcc gttcaccgtg ctgacgtcat cgcgcacgtt gccgccgcga tcgatgaaga
     3601 tgccaccatt gacggtttcc acgtcgccgt cgatgcgcgc ctgcttgccg aagcggatcg
     3661 cgccattgac ggtcgacacc gaatgcgcgc cgacatcgtc cgcggcctcg atgccgccgt
     3721 tcacggtttc cacgtcgcgc actcgcgcgc cgcgttccag gtcgatgccg ccattgacgg
     3781 tgctgaggtc gccgtaggtc tcgccggcgc tggcggtgat gctgccgttg accttgtcga
     3841 tgtcgcgacc gggaccctcg tggtcatgcg caggggcgac gctggagaag gcaagcaggg
     3901 cgacgaagag cagggagcgt ttcatgcggg actccagtgg ttgtacagcc tctgatgcag
     3961 caggccggcc aagggtttag acaccgctac aatcatcgga cgcccgccgc gcgggcctcc
     4021 cccacaggaa ccgatcgtgc ccgccaacgc tcccgtccgt cccaagcccg tgatgctgtt
     4081 gatcctggat ggctggggcc accgagagga ggtcgaggat aacgcgctgg cccaagccga
     4141 cctgcccaat tggcgccacc tgctggcgac ctgcccgcac acgctggtgc ataccgaagg
     4201 ccgtcatgtc ggtctgccgg acgggcagat gggcaattcc gaggtcggcc acatgaacct
     4261 gggcgccggc cggatcgtct accaggacct gacccgggtc gatgccgcga tcgaggacgg
     4321 cagcttcttc gccaatgccg aattgaatgc cgcgtgccgg gacgccatcg cccacgacgg
     4381 cacgctgcac gtgatgggcc tgctttcgcc cggcggcgtg cacagccacg aagcgcacat
     4441 cttcgcgatg ctcgacctcg cccatcgcgc gggcgtgccg cgggtcgcgg tgcatgcgtt
     4501 cctggatggc cgcgacaccc cgccacagtc cgccgaggcc agcctgcgcg ccctgcaggc
     4561 gctctgcgac aggctgggca acgcacgcat cgccagcgtg ggcggtcgtt acttcgcgat
     4621 ggatcgcgac aagcgctggg atcgcgtgcg gcgtggctgg gacgcgatcg tcgaggccgc
     4681 aggcgagcat cgcgcgaccg acgctctgtc cgcgttgcag gccgcctatg cgcgcggcga
     4741 gaacgacgaa ttcgtggcgc cgaccgtgat cggcgccgca tcgcccatgc gggatggcga
     4801 tgcggtggtg ttcatgaatt tccgtgccga ccgtgcgcgc caattgaccg cggccttcgt
     4861 cgatcccggc ttcgaagggt tcgtcgcacg ccgccccgcg ctgtcgcgct tcgtctgcct
     4921 gagcgaatac gacgcgaagc tgcccgcgcc gctcgcgttc gggccggacg acctgcgcca
     4981 caccttcggc gagatcgtgg cggatgccgg gctcacccaa ctgcgcatcg ccgagaccga
     5041 gaagtacgcg cacgtcacct tcttcttcag cggcggccgg gaggcgctgt tcgccggcga
     5101 ggaacgcatc ctggtgccca gcccgcaggt cgccacctac gacctgcagc cggaaatgag
     5161 ttgcccggag ctgaccgaca agctggtggc cgcgatcggc tccggccggt tcgatgcgat
     5221 cgtgtgcaac atcgccaatc cggacatggt cggccacagc ggcatccttg ccgcggcgat
     5281 cgccgcggcg caggcggtcg acatcgcgat cggccggatt gcgcaggccg tgcgcgaggt
     5341 cggcggcgcg ctgatcatca ccgccgacca cggcaacctg gaaatgatgc gcgacccggc
     5401 aacgggccag ccgcataccg cgcatacggt aggtccggtg ccgctggtgt acgtgggcga
     5461 tcgcgatgtc gcgctgcgcg gcggtggcgc gctgcgcgat gtcgccccga ccctgctcga
     5521 cctgatgaat gtgcagcccc cggccgagat gagcggccag agcctgctgc tgccggcctg
     5581 atgcgccgat tgcgcgccgt cgcgttgtgc gccgtgctgg cgatgctggc cggcgttgcc
     5641 catgcccagg atcgcagcga tgccgagcgt cgcttgcagg gggtacgcaa cgagttgaag
     5701 caggtcgcgg ccgagcgccg caagctggag ggcgagcgcg gcgatgccag ccgcaagttg
     5761 cgcgaggctg acgagcaggt cggcggcgtc cagcgctcgt tgcagaagac ccagaccacc
     5821 ctgcaacgcg atggcgcgga gttgctacgc ctgcaggcgg aacgcgccag gcatgtgggc
     5881 gacctggagg ccaagaagac cgagctggcg cgcctgttgc gcgccgcgca attcgccggc
     5941 agcgaagcgc cgttgaagac gatgctggcg caggatcgcg tggcggaggc cgaacgcgcc
     6001 ttgacctacc agggctacct gcagcgcggc caggtgcagc ggatccgcgt gctgtcggcg
     6061 gaactgcagc gcatcgacgc actggagcgc gagatcgtcg cgcgccgcga ggcgctggac
     6121 cgcgagcgac gcacgcaggc cagtcaactg accgagctgc agcaggcgcg caagcagcgc
     6181 gcggggttgc tggccggcat cgaccagcaa tatcgggatc gcgcgggacg cgagaaggcg
     6241 ctgggacgcg acgccaaggc cttgcaggac ctgctgtcgc gcctgcgcgc ggcggcggcg
     6301 aaagccgccc gcgacgccgc ccgcgccaag gcggaggcgg agcgccaggc cgtggcctcg
     6361 ggcaagccca agccgaaacc ctcggccaga cgcgcgacgg tggccagtgc gccagcgatg
     6421 cgcgtgggtg gcctgggttg gccggtggcc ggcaacctgc tggcctcgtt cggcggcacg
     6481 ttgccggacg gccgacgcag cgacggcgtg ctgatcgccg cggcggccgg caccgcggtc
     6541 aaggcgatgg ccgatggcac cgtggtctac gccgactgga tgaccggcta cggcaacatc
     6601 ctgatcatcg accacggcaa tggctacatg agcctgtacg cgcacaacga cggcctgtcg
     6661 cgggatgccg gcgacgtggt gaagcgcggc gataccgtgg ccagcgtggg cacctcgggc
     6721 gggcaggacg tgcccgcgtt gtacttcgaa ctgcgtcgaa atggtgcgcc ggtcaatccg
     6781 gccagctggc tgacgcggca gtgaacgcgt gcacggcatc gcaacgcctt tcttgggcat
     6841 gatggggaac ccggtcgaca ccgatcggtc ggacacccag ccgcaacgcg caaccggaga
     6901 tcccatgcgg gccaagccgc tttccctcgt catcgccatc ctgctgggcg tggccgccgc
     6961 gccgtctttc gcgcagtcgc cggcgtccgc acctgcggca cctgccgcca cggagcctgc
     7021 ggctgcgccg cagggcgagc aggatccact ggccagcgac gatcccgact cggccaagac
     7081 cgatgccgcc aaggtgccgc tggacgagat ccgccgctac gtgatggtct acaacgcgat
     7141 ccgccaggcc tacgtggatc cggtcgacga tcacgcactc atgcagtccg cggtgcgcgg
     7201 cctgctgctg gacctggacc cgcacagcgc ctacctcaag aaggcggatg ccgaggaatt
     7261 cgacgagctc accagcggcg cctacgacgg cgtgggcctg gagttgcagc agttgccgga
     7321 aggcggcgtg cgcatcgtgg cgccgttgga cgatggcccc gccgaacgcg ccggcctgcg
     7381 cagcggcgac ctgatcatcg cgatcgacgg caagccgctg gaagcgggcg cgggcgacgc
     7441 ctccgcaccg ctgcgcggca aggccgggac caaggtcgtg gtgcgcatcg tccgcaaggg
     7501 cacggcgcaa ccgttcgacg tgaccctggt gcgcgacacc atccgcacca ccagcgtgcg
     7561 ctcgcggatg ctggagccgg gcttcggcta tgtccgcctc agcagtttcc aggcggccac
     7621 cgccagcgac ttcaacaagg cggtggacg
//
LOCUS       DAOOCX010000104         7407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_9402_length_7407_cov_6.978917, whole genome shotgun sequence.
ACCESSION   DAOOCX010000104 DAOOCX010000000
VERSION     DAOOCX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9402_length_7407_cov_6.978917"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /gene="nadC"
                     /locus_tag="PK399_09460"
     CDS             <1..813
                     /gene="nadC"
                     /locus_tag="PK399_09460"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPW13385.1"
                     /translation="LAEDLGPGDATASLLDDTPDRAYLLCKEDCVVAGRPWFDAVHRA
                     LDPGVRIDWRCDEGDHVGRGTVIATLQGRNRALVSAERASLNFLQTLSGTATTTAHYV
                     DAVRGTGTKILDTRKTIPGLRLAQKYAVRVGGGMNHRIALYDAVMLKENHIRAFGSVA
                     AAIARARALQPGLPLIVEVETLAQLREALDTGCTRILIDDFDADTRREAVRIAKGAPY
                     LGRIPLEVSGGVDMTTLRGIAEDGVDCISIGGLTKHLHAIDFSLKLGPPPGG"
     gene            complement(818..1576)
                     /locus_tag="PK399_09465"
     CDS             complement(818..1576)
                     /locus_tag="PK399_09465"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPW13386.1"
                     /translation="MKLVFQGGEHPPFPLPDGACSVGSALDADIALGRDGIEPLHCEL
                     LVGAHGVRLRVIPGARVLVNDRPVDGVIALRANDALGLAEVKARLVEVVEELPRAPAP
                     QVGVERGELNATMVRPVVPKFALRAVSGALFGRSFPLQSILSIGRADDAGLRIPLDSI
                     SRQHARLTPAGNEVLVEDLGSANGTWINGKRITRASAKHGDEISFDKERFQLLEPGQP
                     AVVPRRSRGMGRARWPWVLAALLAGAALATWLIL"
     gene            1643..1981
                     /locus_tag="PK399_09470"
     CDS             1643..1981
                     /locus_tag="PK399_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HPW13387.1"
                     /translation="MPTLLILMIVGVIAFFTFSAARAAAERAALIGRDACQAAGVQWL
                     DQSTHATGMRLRRKEDGWLGWERSFRFDYSEDGQDRHIGRIVLLGDKLVGFSGPSRAA
                     QAVVRSFPGH"
     gene            complement(2099..2572)
                     /locus_tag="PK399_09475"
     CDS             complement(2099..2572)
                     /locus_tag="PK399_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HPW13388.1"
                     /translation="MGDTAPPAMSSQRPYLLRALYEWIADNGMTPHVLVDARMPGVRV
                     PAHAVKDGRIVLNIAERAVAKLELGNDALRFSARFAGVSQAVLVPLEAIIAIYARETG
                     QGMALPEEPRPSASQVDQGEVVEQVDDAPGAVDSPESPDTPPEPPKRGGHLRIVK"
     gene            complement(2585..3220)
                     /locus_tag="PK399_09480"
     CDS             complement(2585..3220)
                     /locus_tag="PK399_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HPW13389.1"
                     /translation="MAVSPRMRNALTLFSSVDDVLCHRVRLVLAAKGVTYDFVSVDPQ
                     NPPEDLIDLNPYHSVPTLVERDLVLYAASVVSEYLDERYPHPPLMPVDPLSRARLRLA
                     MLRIEHDWVPQVQAIRMGNKQQVEAARKRLKELLTASVPLFKASKFFLNQEMSLADCA
                     MAPIIWRLQSLGIPLPKDGKAIEDYGNRIFRNPGFLRSLTEQEKALRDLPL"
     gene            complement(3332..4093)
                     /locus_tag="PK399_09485"
     CDS             complement(3332..4093)
                     /locus_tag="PK399_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017113706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HPW13390.1"
                     /translation="MTNLPSKKRAFVSRLAVFAAGVLLSTVALASGGGNLQQAGTDLT
                     DKASLQRGAQLYMNYCSGCHSLKYVRYARIGEDLGLSEDDVQANLNFNGGKIGDHVIS
                     RMTAEMGVAAFGKAPPDLSVIARVRGSDWIYTYLKSFYLDESRPVGWNNTLFANASMP
                     NPLWELQGLQQPVHGKKTASGELPVEGFTISQAGTQDAQAFDQTARDITAFLEYAGEP
                     AALKRESLGVWVILFLVFLAFLAWLLKNEYWQDVH"
     gene            complement(4086..5369)
                     /locus_tag="PK399_09490"
     CDS             complement(4086..5369)
                     /locus_tag="PK399_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007965674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HPW13391.1"
                     /translation="MANFLIRTATDAANGAMEWVNQRAPGMMPFYRKHMSEYYAPKNF
                     NIWYIFGILATVVLVNQIVTGIFLTMHFKPSAAEAFASVEYIMRDVEWGWLIRYMHST
                     GASLFFVVVYLHMFRGLMYGSYKKPRELVWLLGMLIYLVLMAEAFLGYVLPWGQMSYW
                     GAKVIISLFGAFPVIGTGLTEWIMGDYNPGDATLNRFFALHVIALPLVLLLLVVLHLF
                     ALHEVGSNNPDGVEIKKGPKGNRWSPTAPADGVPFHPYFTVKDTFYVCCFLVIAAFII
                     FYAPAFGGWFLEHDNFTEANALVTPAHIKPVWYYTPYYAMLRVVPSFFGIKLWGVLVM
                     FGAIAVLFLVPWLDKSPVKSYRYRGWLSWGMLLMFAVCFLWLGKIGAGPGTDPTETII
                     GRVLTFLYFAFFITMPVWTKLDKTKPVPERVTMHD"
     gene            complement(5373..5972)
                     /gene="petA"
                     /locus_tag="PK399_09495"
     CDS             complement(5373..5972)
                     /gene="petA"
                     /locus_tag="PK399_09495"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HPW13392.1"
                     /translation="MTNHEVNTGRRRFLTATTAVVGAVGAGFAAVPFIKSWSPSARAR
                     FAGAPVSQDLSALAEGQQLVINWRGQPIFIAKRSKAMLETMKSLDSLLADPGSANADQ
                     QPKYAQNATRSIKPEISVLVGVCTHLGCAPEFLPEVKPEPFDPDWKGGYFCPCHKSRY
                     DLAGRVLKAQPAPANLPVPPYYFENDTTVVIGVDPKGAA"
     gene            complement(6072..7040)
                     /locus_tag="PK399_09500"
     CDS             complement(6072..7040)
                     /locus_tag="PK399_09500"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPW13393.1"
                     /translation="MKGLAAVLFSTLLLATPALAGNVYRCEGSDGVTTYANKKVAGAS
                     CRLVGSYKAQRAPRPKVAAAPPKTSSSQRGSESPPPGGVPPATMAASQLAPSSVPTAA
                     DIPAPAVPVAAPVPAGPRRVHGQVYSYMKDGVRTYTSARPRGMSLASVRTIKYSYMET
                     CYACGTKPGVNFRTLRLNTTAYQAEIAAAAREHGVDESIVRAIIHAESAFNPNALSRV
                     GAQGLMQLMPATARRFGVGNAFDANQNIRGGVQYLAWLLKRFNGNLTLAAAGYNAGEG
                     AVAKYGGVPPYSETRRYVERVGLLADRYRGLGNPVAAPTPGGLSAR"
     gene            7208..>7407
                     /locus_tag="PK399_09505"
     CDS             7208..>7407
                     /locus_tag="PK399_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPW13394.1"
                     /translation="MSQPEPQVADAPARKKLFIETHGCQMNEYDSARMADVLAATDGM
                     ELTTIAEEADVILVNTCSIREKA"
BASE COUNT         1254 a         2491 c         2474 g         1188 t
ORIGIN      
        1 ctggccgagg acctcggccc cggcgatgcc acggcgtcgc tgctcgatga cacgccggat
       61 cgcgcgtacc tgctgtgcaa ggaggactgc gtcgtggccg gccggccgtg gttcgatgcc
      121 gtccatcgcg cgctggaccc aggcgtgcgc atcgactggc gttgcgacga gggcgaccac
      181 gtcggcaggg gcacggtcat cgccaccctg cagggccgca accgcgcgct ggtcagcgcc
      241 gagcgcgctt cgctgaactt cctgcagacg ttgagcggca ccgccaccac cactgcccac
      301 tacgtggatg cagtgcgcgg caccggcacg aagatcctcg atacccgcaa gaccatcccc
      361 ggcctgcgcc tggcgcagaa gtacgcggtg cgggtgggcg gcggcatgaa ccatcgcatt
      421 gcgctctacg acgcggtgat gctgaaggaa aaccatatcc gcgcgttcgg ctcggtggcc
      481 gcggcgatcg cgcgcgcccg cgcgctgcag ccgggactgc cgctgatcgt cgaggtggag
      541 accctggcgc agttgcgcga ggccctggac acaggctgca cccgcatcct catcgacgac
      601 ttcgatgccg atacgcgccg cgaggcggtg cgcatcgcca agggtgcgcc ctacctggga
      661 cgcatcccgc tggaagtctc cggcggcgtg gacatgacca cgctgcgtgg catcgccgaa
      721 gacggcgtgg actgcatctc gatcggcggg ctgaccaagc acctgcacgc gatcgacttc
      781 tcgctgaagc tgggcccgcc gccgggcggt tgatccgcta gaggatcaac caggtcgcca
      841 gtgcagcgcc tgcgagaagt gccgccagca cccacggcca gcgtgcacgg cccatgcccc
      901 tggaacgcct cggcacgacc gccggttgcc cgggttccag caactggaag cgctccttgt
      961 cgaagctgat ctcgtcgccg tgcttggcac tggcgcgggt gatccgcttc ccgttgatcc
     1021 aggtgccatt ggccgagcct aggtcctcga ccagcacctc gttgccggca ggcgtcaacc
     1081 gggcgtgctg ccgcgagatg ctgtccagcg gaatccgcag gccggcgtcg tccgcgcgcc
     1141 cgatgctgag gatcgactgc agcgggaaac tgcgcccgaa caacgcaccc gataccgcac
     1201 gcaacgcgaa cttcgggacg accgggcgca ccatcgtggc gttgagttcg ccgcgctcga
     1261 ccccgacctg gggagcgggc gcgcgtggca gctcttcgac cacctcgacc agccgggcct
     1321 tcacctcggc cagccccagg gcatcgtttg cgcgcagggc gatcacccca tccacgggcc
     1381 ggtcattgac cagtacccgc gcgcccggga tgacccgcag cctcacgccg tgcgcaccga
     1441 ccagcagctc gcagtggagc ggttcgatcc cgtcgcgccc gagcgcgatg tcggcgtcga
     1501 gcgccgagcc gaccgaacag gccccgtcgg gaagaggaaa cgggggatgt tccccgccct
     1561 ggaataccag cttcattgcc atgtgcgcgc gttccccctg tccatgcttg cgctgaggtc
     1621 ccggcaccac cacattgggc ggatgccgac cttgctgatt ttaatgattg tgggcgtcat
     1681 cgccttcttc acgttctccg ctgcgcgcgc agcggcggag cgcgcggccc tgattggccg
     1741 tgacgcatgc caggccgcgg gcgtgcagtg gctggatcaa tccacgcatg ccaccgggat
     1801 gcgcctgcgc cgcaaggagg atggttggct cgggtgggag cgcagtttcc gcttcgatta
     1861 ctcggaagat ggccaggatc gccacatcgg gcggatcgtc ctgctcggcg acaagctggt
     1921 cggcttcagt ggcccgagcc gggcagcgca ggccgtggtg cgcagtttcc ctggtcattg
     1981 acggccgcag gcaaacccgt ggcttcgttc gggcgaccgg tcgttgcata gcggcaatcc
     2041 cctgtcgttg cctgctctcc cgacaagacc aaggcaagca cgggggcctg cccgtcggtc
     2101 atttcacgat gcgcaggtgg ccaccgcgct tgggcggttc cggcggggtg tccggcgatt
     2161 cgggcgaatc gaccgccccc ggggcgtcgt cgacctgctc gacgacctcg ccttggtcca
     2221 cctgcgacgc actggggcgg ggttcctccg gcaacgccat gccctggccg gtttcccgcg
     2281 cgtagatcgc gatgatcgcc tcgagcggga ccagcacggc ctggctgacg ccggcgaagc
     2341 gggcgctgaa acgcagggcg tcgttgccga gttcgagctt ggccaccgcg cgttcggcga
     2401 tgttgagcac gatgcgtcca tccttcaccg cgtgcgccgg cacgcgcacg ccgggcatgc
     2461 gcgcatcgac cagcacatgc ggggtcatgc cgttgtcggc gatccattcg taaagcgcgc
     2521 gcaacaggta cgggcgctgg ctggacatcg cgggcggggc ggtatcgccc atctcaatcc
     2581 accatcacaa cgggaggtcg cgcagggcct tttcctgctc ggtcaggctg cgcaggaaac
     2641 cggggttgcg gaagatccgg ttgccgtagt cctcgatcgc cttgccgtcc ttcggcaggg
     2701 ggatgccgag cgactgcagg cgccagatga tcggcgccat cgcgcagtcg gccaggctca
     2761 tttcctggtt gaggaagaac ttgctggcct tgaacagcgg caccgatgcc gtcagcagtt
     2821 ccttcaggcg cttgcgcgcg gcttcgacct gctgcttgtt gcccatccgg atcgcctgca
     2881 cctgcggcac ccagtcgtgc tcgatgcgca gcatcgccag gcgcagccgc gcgcgggaga
     2941 gcggatcaac cggcatcaat ggcggatgcg ggtagcgctc gtccaggtat tcgctgacca
     3001 cgctggccgc gtacagcacc aggtcgcgct cgaccagggt cggcaccgag tggtagggat
     3061 tgaggtcgat caggtcttcg ggcgggttct gcgggtcgac cgacacgaag tcgtaggtga
     3121 cgccctttgc tgccagcacc aggcggacgc ggtggcaaag gacatcgtcg accgaggaaa
     3181 acaaggtcag tgcgttacgc atacgcggac tcacagccat catcaggaca actcctgcgc
     3241 ccgggaatcc cggtcgcacc tgcgccgccc gcacgatgcg gacggccgca aagcgtactt
     3301 caccctgttc gtcgcacctg ccgccgtcgt gtcagtggac gtcctgccag tactcgttct
     3361 tcagcagcca ggccaggaac gccaggaaga ccaggaacag gatcacccac acgcccaggc
     3421 tttcgcgctt gagtgcggcg ggctcgccgg cgtattccag gaaggcggtg atgtcgcgcg
     3481 cggtctggtc gaatgcctgc gcatcctgcg tgccggcctg gctgatggtg aacccctcga
     3541 ccggcagttc gccgctcgcg gtcttcttgc catgcaccgg ctgttgcagg ccctgcagtt
     3601 cccacagcgg gttcggcatc gaggcgttgg cgaacagcgt gttgttccag cccacgggac
     3661 gcgattcgtc caggtagaac gacttcaggt aggtgtagat ccagtcgctg ccgcgcacgc
     3721 gcgcgatcac gctcaggtcc ggcggtgcct tgccgaacgc ggccacgccc atctccgcgg
     3781 tcatccgcga aatcacgtgg tcgccgatct tgccgccatt gaagttgagg ttggcctgca
     3841 cgtcatcctc ggagaggccc aggtcctcgc cgatgcgggc atagcgcacg tacttcagcg
     3901 aatggcagcc gctgcagtag ttcatgtaca gctgcgcgcc gcgctgcaga gaggccttgt
     3961 cggtcaggtc ggtgccggcc tgctgcaggt ttccgccgcc ggaggccagc gccacggtcg
     4021 acagcaggac accggcggcg aacaccgcca ggcgggagac gaacgcccgc ttcttggaag
     4081 gcaagttagt catgcatcgt cacccgttcc ggcaccggct tggtcttgtc gagcttcgtc
     4141 cacaccggca tggtgatgaa gaaggcgaag tagaggaagg tcagcacgcg gccgatgatc
     4201 gtctcggtcg gatccgtgcc cgggccggcg ccgatcttgc ccagccacag gaagcagacc
     4261 gcgaacatca gcagcatccc ccagctcagc cagccacggt agcgatacga cttcaccggc
     4321 gacttgtcga gccagggcac caggaacaac accgcgatcg cgccgaacat caccagcacg
     4381 ccccacagct tgatgccgaa gaacgagggc accacgcgca acatcgcgta gtacggggtg
     4441 tagtaccaca cgggtttgat gtgggccggc gtcaccaggg cgttcgcctc ggtgaagttg
     4501 tcgtgctcca ggaaccagcc gccgaaggcc ggcgcgtaga agatgatgaa cgccgcgatg
     4561 accaggaagc agcagacgta gaacgtgtcc ttgaccgtga agtacgggtg gaacggcacg
     4621 ccgtcggcgg gcgcggtcgg cgaccagcgg ttgcccttcg ggcctttctt gatctcgacg
     4681 ccatcgggat tgttggagcc gacctcgtgc aacgcgaaca ggtgcaggac caccagcagc
     4741 aacagcacca gcggcagcgc gatcacgtgc agcgcgaaga agcgattcag cgtggcgtcg
     4801 cccgggttgt aatcgcccat gatccattcg gtcaggccgg tgccgatgac cgggaacgcg
     4861 ccgaacaggg agatgatcac cttggcgccc cagtacgaca tctggcccca ggggagcacg
     4921 tagccgagga aggcctcggc catcagcacc aggtagatca gcatgcccag cagccagacc
     4981 agctcgcgcg gcttcttgta ggagccgtac atcaggccgc ggaacatgtg caggtagacc
     5041 accacgaaga acagcgacgc gccggtgctg tgcatgtagc ggatcagcca gccccactcc
     5101 acgtcgcgca tgatgtactc gaccgaggcg aaggcctccg ccgcgctcgg cttgaagtgc
     5161 atcgtcagga agatgccggt gacgatctgg ttgaccagca ccaccgtggc caggatgccg
     5221 aagatgtacc agatgttgaa gttcttcggc gcgtagtact ccgacatgtg cttgcggtag
     5281 aacggcatca tgccgggcgc gcgctggttg acccattcca tcgcgccgtt ggcggcgtcg
     5341 gtggcggtgc ggatgaggaa gttcgccatg gcttacgcag cccccttcgg atcaacgccg
     5401 atgacgacgg tggtgtcgtt ctcgaagtag tacggcggca ccggcaggtt ggccggtgcc
     5461 ggctgcgcct tcagcacgcg gccggccagg tcgtagcgcg acttgtggca ggggcagaag
     5521 tagccgccct tccagtccgg gtcgaacggc tccggcttca cttctggcag gaattccggg
     5581 gcgcagccca ggtgcgtgca cacgcccacc aggaccgaga tctccggctt gatcgagcgc
     5641 gtggcgttct gcgcgtactt gggctgctgg tcggcgttcg ccgaaccggg atccgcaagc
     5701 agcgaatcca gcgacttcat ggtctccagc attgccttgg agcgcttggc gatgaagatc
     5761 ggctggccac gccagttgat caccagttgc tgcccttcgg ccagggcgct gaggtcctgg
     5821 cttaccggtg caccggcgaa acgggcgcgg gcactggggc tccacgactt gatgaaaggc
     5881 accgcggcga accccgcccc gaccgcaccg accactgccg tggtcgcggt caggaacctg
     5941 cgccggccgg tgttgacctc atgattggtc atcccgcact ccgaaaataa gcaaatacac
     6001 gactgcggtg cccggcgacg tgctggcgca cgctgaccgg ccgcaaaaga cggggaagtg
     6061 taacggaacg cttaacgagc cgacaatccg cccggggtcg gcgccgcgac gggattgccc
     6121 agtccccggt aacgatcggc cagcaggccg acccgttcca cgtagcgccg ggtctcgctg
     6181 tagggcggca cgccgccgta cttggccacc gcgccctcgc ccgcgttgta acccgcggcg
     6241 gccaaggtca ggttgccgtt gaagcgcttc agcagccacg ccaggtactg cacgccgcca
     6301 cggatgttct ggttggcgtc gaacgcattg cccacgccga agcggcgcgc ggtggccggc
     6361 atcagctgca tcagcccctg cgcaccgacc cgcgacagcg cgttggggtt gaaggcggat
     6421 tcggcatgga tgatggcgcg gacgatcgat tcatcgaccc cgtgctcgcg cgcggcagcg
     6481 gcgatctcgg cctggtacgc ggtggtgttc aggcggaggg tgcggaagtt cacccccggc
     6541 ttggtcccac aggcgtagca ggtctccatg tagctgtact tgatggtgcg caccgaggcc
     6601 agcgacatgc cgcgcgggcg ggcgctggtg taggtgcgca cgccgtcctt catgtaggaa
     6661 tagacctgtc cgtgcacccg ccgcggcccc gcgggcaccg gggctgccac gggcaccgca
     6721 ggcgccggga tgtccgcggc ggtcggcacc gacgacgggg caagttgcga agccgccatc
     6781 gtggccggag gcacgccacc aggtggcggg gattcgctgc cgcgttgcga cgatgacgtc
     6841 tttggcggcg cggccgcgac cttcggcctg ggcgcacgct gcgccttgta actgccgacc
     6901 agcctgcaac tggcaccggc caccttcttg ttcgcgtacg tggtcacccc gtcactgcct
     6961 tcgcagcgat acacgttccc cgccagcgcc ggcgtggcca gcaacaaagt cgaaaacagg
     7021 acggcggcca accccttcat ccgcgcagtc tcgccgctcc gatttgcgat gacaagtcct
     7081 tgattttcct gaacgccgca gcgcgcgagc gcagcccagc cggtggcgaa ccggcgcagc
     7141 cggggcgttt gctaagctcg cgcccccaaa ccggcccgga ttaaccgccg cgccccggag
     7201 agaacccatg agccagcccg aaccgcaggt cgcggacgcg cccgcacgca agaaactgtt
     7261 catcgagacc cacggctgcc agatgaacga gtacgactcg gcgcggatgg ccgatgtgct
     7321 ggccgcgacc gacggcatgg aactgaccac gatcgcagaa gaagccgacg ttatcctggt
     7381 caatacctgc tcgatccgcg aaaaagc
//
LOCUS       DAOOCX010000105         7387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_9441_length_7387_cov_7.160529, whole genome shotgun sequence.
ACCESSION   DAOOCX010000105 DAOOCX010000000
VERSION     DAOOCX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9441_length_7387_cov_7.160529"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK399_09510"
     CDS             <1..231
                     /locus_tag="PK399_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13395.1"
                     /translation="ARREWCINTMVTDPQRKSILANNEDGILYRWDLTTNTFTQNVRL
                     NAGLGQAYTPTWVGADGGVYAISNATLYAIRA"
     gene            complement(228..1376)
                     /locus_tag="PK399_09515"
     CDS             complement(228..1376)
                     /locus_tag="PK399_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HPW13396.1"
                     /translation="MRSIRRLLVGVAILALAACSREPGPPATPAGIAAATTIDFAHVA
                     NYAAPPLPAYFDETVAALDNSPASNAADDRVATLGRVLFHDMRLSTNNRTSCAGCHQQ
                     RFGFTDPMRFSNGISTAGTTDFHAMRLGNLRYWRPGTMFWDRRVVSAEVQASHPLHSL
                     VEMGWGGNVGGIDALIRKMSATDDYPALFAWAFDSPTITEPRMQQALAQFVRAMVSHD
                     SRWDAGYAQVFSPTAPNRALDVDLANFTAEENRGRHLFMTDVRQGGAGCAACHRPPTF
                     ALAADARSNGLDAGETRLFKAPSLRSVGLTGPYMHDGRFATLEEVVDFYTSGVRDGPA
                     LDPRLREGREPRRLDLDAGDRAALVAFLKTLDDPVLTTDPRFSDPFRR"
     gene            1645..2829
                     /locus_tag="PK399_09520"
     CDS             1645..2829
                     /locus_tag="PK399_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13397.1"
                     /translation="MRGYRDGVATRLVHPDSWSNLAALAIALQDPAAARQHAMHALQL
                     DARHVDAWVNLGVASWHAGQRREGAQATNQALQHVPGLEAAALNFALMLRVVGRLAQA
                     HDVLAGAVHANPGAWRLHLALAGTARLLERHDVARREVLVALDRMPLAATHDSVARAR
                     PLADNHGELVTQALVATADAFHAAGLPFHLIGGTLLALYRDGAPFPHDKDIDLGVPAD
                     VDRPAVYRALGQGFSPMLPRDDARALASREWVLGFIHDATGIGVDLMFMHLRGHVMRL
                     GMGWPDHIDSEVPAYGVEMLRWQDRDWPVPSPPDRFLAAIYGDDWRTPQRHYDTQVSN
                     PSRTPESLPRAITLGLIRLAEAVRAGQWSKADALCQQILAREDVAEVRRLRARLATAG
                     VA"
     gene            2832..3497
                     /locus_tag="PK399_09525"
     CDS             2832..3497
                     /locus_tag="PK399_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPW13398.1"
                     /translation="MAGADGMVSCLIPAAGAGERLGQGTKGLLELRGRPLLCWLADKA
                     LQVADEVLVAVPGGRVEAVARLLPGCRVLAGGDTRHDSVALLAEHARGEWLLLQDVAR
                     PFASVGLMRRVIEAARTHGCAGAFVDPEVPVARLRDGFAVEALPRHEAGVFQSPQAFA
                     RVQLLALIGQARADGWRPQSTLQLALRAGVPVAAVAGEKHNIKITTAEDWRWACAMEE
                     YLS"
     gene            3494..4729
                     /locus_tag="PK399_09530"
     CDS             3494..4729
                     /locus_tag="PK399_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HPW13399.1"
                     /translation="MSSGNMPHTRHPELFLWSEALDPVDYVVARYRVASLHDGDATAM
                     AMAMEQSAATTHIAGHVEPSMLRDWTLRVRSVRSIESLGGSGLAPYHLATEVYADGGD
                     AERHWAVELAIPRRLISGKPAQLWNVVVGEIPRLGFLTRFQLSGLDLPADFGPGPGFG
                     VAGIRRQFGCERGPLLCRSMRPAVGLDIPTMAGLNRAVLEGGFHLVKDDELQVFADEA
                     AFRAHVQAMLAARDAARASTGQRKGYLANLICEPDELQARWEIACTLGVDAVLVAPGI
                     QGLGTLATLARQKRMPILAHNTFSDLLTRHPDWGIAHPVLSGLYESFGADWIVTSGEY
                     GASDGVGMDDAWPFGRPQRAMPILQGGKSPEGLPTFRAAIGSDDYMLIVASWVDGHAD
                     GLGNAAGIFRAAVDAQLPA"
     gene            4739..5407
                     /locus_tag="PK399_09535"
     CDS             4739..5407
                     /locus_tag="PK399_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPW13400.1"
                     /translation="MGDARTGVSFLMLAAGAGERLGLGPKGSLELQGRPLLCWLADKA
                     VQVAEDVLVAVPAEHVEAIAALLPQCRVIAGGATRQDSIALLAAKASGEWLLLHEAAR
                     PFASLALFRQVIAMARHHGCAGAFLSPVVPVARLRDGRVAEAYAGSEVGIFQTPQVYA
                     RADMQRMLQHQASVPGWFAQSPVQLALAAGVEVHVVPGEACNLKITTADDWRMARHLA
                     HLLA"
     assembly_gap    5672..5771
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5772..6298
                     /locus_tag="PK399_09540"
     CDS             <5772..6298
                     /locus_tag="PK399_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13401.1"
                     /translation="NAELGVPVLKLDNTVNAQDFVVVSLPGRAPVDTKAISNGMAIQR
                     FLKGKQGLNASIVYEFAPGTVAETDSRMLGTEAGAGLWFNARADFIGAERGGWGNINT
                     KPEGLVVAPDIGTFGEIAGSKAGTAENVIRFIGGMGGVDRTGYQMTPDWARAEAAMLA
                     AGKAFNAELVARLK"
     gene            6345..>7387
                     /locus_tag="PK399_09545"
     CDS             6345..>7387
                     /locus_tag="PK399_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW13402.1"
                     /translation="MTTRTNADVLIIGGGHNGLVCAAYLAATGLKVRVLERRHLVGGA
                     AVTEEFHPGFRNSVASYTVSLLNPKVIADLRLAQHGLRVVERPYANFLPLPDGRSFRL
                     GGAHTDSEIAKFAPGDVARLADYTAMLDRVVAVLKELIHLTPPNIGERIAFADWLGSY
                     AVAKRLKHLDLRGRRDLLDLFTKSAGELLDHYFEGEPLKAVLGWDSVVGNFASPYTPG
                     SAYVLLHHLFGEVNGKTGVWGHAIGGMGAITQAMRKECEARGVAIDTDAEVAKLLVEN
                     ERAIGVVLADGRELRAPTIVSNLNPKLLYTKLVERRQLDDDTAQRIERYRNGSGTFRM
                     NVALGELPDFTAMP"
BASE COUNT         1085 a         2458 c         2625 g         1119 t
ORIGIN      
        1 gcgcggcgcg agtggtgcat caacaccatg gtcaccgatc cgcagcgcaa gtcgatcctc
       61 gccaacaacg aggacggcat cctgtaccgc tgggacctga ccaccaacac gttcacgcag
      121 aacgtgcggc tcaatgccgg cttgggacag gcctacacgc cgacctgggt gggcgcggac
      181 ggcggggtct atgcgatcag caacgccacg ctctacgcca tccgggccta acgccggaac
      241 ggatcgctga agcgcggatc ggtggtgagc accgggtcgt ccagggtctt caggaaggcg
      301 accagtgccg cgcggtcgcc cgcatccagg tcgaggcggc gcggctcgcg cccttcgcgc
      361 agtcgcgggt cgagcgccgg accgtcgcgc acgccggacg tgtagaaatc gaccacctcc
      421 tccagcgtcg cgaagcggcc gtcgtgcatg tacggcccgg tcaggccgac gctgcgcagg
      481 gacggcgcct tgaacagccg cgtctcgccc gcgtccaggc cgttgctgcg cgcgtccgcg
      541 gccagggcga aggtcggcgg ccggtggcag gcggcgcagc ctgcgccgcc ttggcggaca
      601 tcggtcatga acaggtggcg gccgcgattt tcctccgcgg tgaagttcgc caggtcgacg
      661 tccagcgcgc gattcggcgc cgtgggcgag aacacctgcg catagccggc atcccagcga
      721 ctgtcgtgcg acaccatcgc ccgcacgaac tgggcgagcg cctgctgcat gcgtggttcg
      781 gtgatcgtcg ggctgtcgaa tgcccaggcg aacaacgccg ggtaatcgtc ggtcgccgac
      841 atcttgcgga tcagggcgtc gatgccgccc acgttcccgc cccagcccat ctcgaccagg
      901 ctgtgcagcg gatggctcgc ctgcacctcc gcactgacga cgcgtcggtc ccagaacatg
      961 gtgcccggtc gccagtaacg caggttgccg agccgcatgg catggaaatc ggtggtgccg
     1021 gcggtgctga tcccgttgct gaagcgcatc ggatcggtga agccgaagcg ttgctggtgg
     1081 cagccggcgc aggaggtccg gttgttggtg ctcaggcgca tgtcgtggaa cagcacccgg
     1141 ccgagcgtcg ccacccggtc atccgcggca ttcgacgccg ggctgttgtc cagcgccgcc
     1201 accgtctcgt cgaagtaggc agggagcggc ggcgcggcgt agttggcgac atgcgcgaag
     1261 tcgatcgtgg tcgccgccgc gatgcccgcg ggggtggcag gcgggccggg ttcccggctg
     1321 catgccgcca acgccaggat cgcgacgccg accaacagcc tcctgatgct tcgcaagcgc
     1381 gctccttcct gttcgtccgc aatcgatcct agcccggttt gcccgcgacg gcgctggcct
     1441 gtgcccccaa ggcacggacg gacgacgtca gaaccgccgt tggcacctgc tggcggccgg
     1501 gattcggtaa cctgtcgcgt ctgcacagga gctgccgtgg cgagtctgca attggaggtc
     1561 cgggtttcga aatccccgga gctgaacgcg cacggtgtgc ttcaggcgat ccgcgcggcc
     1621 gagtcgggcg acacggccgc ggccatgcgt ggttatcgcg acggggtcgc gacccgcttg
     1681 gtgcatcccg acagctggtc caacctggcc gccctggcga tcgcgttgca ggatcccgcg
     1741 gcggcgcgcc agcacgcgat gcatgccctg caactggatg cccgccatgt cgatgcctgg
     1801 gtgaacctgg gcgtggccag ctggcatgcc ggccagcgtc gcgagggtgc gcaggcgacc
     1861 aaccaggcgc tccagcatgt ccccgggctg gaagcggccg cactcaattt cgcgctgatg
     1921 ctgcgggtgg tgggccggct ggcgcaggcc cacgatgtcc tggccggcgc cgtgcacgca
     1981 aaccccggtg cgtggcgcct gcacctggcg ctggccggaa ccgcccgcct gctcgaacgc
     2041 catgacgtgg cacgccgcga ggtgctggtg gccctcgacc ggatgccgct ggccgccacc
     2101 cacgattccg tcgccagggc gcgcccgctg gcggacaacc acggcgaatt ggttacgcag
     2161 gcgctggtcg ccaccgccga tgccttccat gcggccgggt tgcccttcca tctcattggc
     2221 ggcaccttgc tggcgctgta tcgcgacggc gcgccgttcc cgcatgacaa ggacatcgac
     2281 ctgggcgtgc cggcggacgt ggatcgcccg gcggtttacc gggcgctggg gcagggcttc
     2341 agccccatgt tgccgaggga cgatgcgcgt gcgctggcgt cgcgggagtg ggtgctgggt
     2401 ttcatccatg acgccaccgg catcggcgtg gacctgatgt tcatgcacct gcggggtcac
     2461 gtgatgcgcc tgggcatggg ctggccggac cacatcgaca gcgaggtacc ggcctatggc
     2521 gtcgagatgc tgcgctggca agatcgcgac tggccggtcc cgtcgccgcc cgatcggttc
     2581 ctcgcggcca tttacggtga cgactggcgg acaccccagc gccactacga cacccaggtc
     2641 tcgaatccct cgcgcacgcc ggaatcgttg ccgcgcgcga tcaccctcgg gttgatccgc
     2701 ctggccgagg ccgtccgcgc cgggcaatgg tcgaaggccg atgccttgtg ccagcagatc
     2761 ctcgcccgcg aggacgtggc cgaagtgcgc cggctgcgcg cgcgcctggc cacggcgggc
     2821 gtggcctgac gatggcgggt gcggatggca tggtgtcctg cctgattccc gcggcgggcg
     2881 cgggcgagcg gctcggccag ggaaccaagg ggctgctgga attgcgcggc cggccgctgc
     2941 tgtgctggct ggccgacaag gccctgcagg tggccgacga ggtgctggtg gcggtccccg
     3001 gcggacgcgt ggaggcggtg gcgcgattgc ttcccggttg ccgcgtgctg gccggtggcg
     3061 acacgcgcca tgacagcgtg gccctgctgg ccgaacacgc acgcggcgag tggctgctgc
     3121 tgcaagacgt ggcgcgtccg ttcgccagcg tcgggttgat gcgccgggtc atcgaggcgg
     3181 cacgcaccca cggttgcgcc ggcgccttcg tggatcccga agtgccggtg gcgcggctcc
     3241 gcgacggctt cgcggtggag gcgctgccgc ggcacgaggc cggcgtgttc cagtcgccgc
     3301 aggcgttcgc ccgcgtgcag ttgctggcgc tgatcgggca ggcccgcgcg gatggctggc
     3361 ggccgcaatc gaccctgcaa ctcgcgctgc gcgccggcgt gccggtggcc gcggtggccg
     3421 gcgagaagca caacatcaag atcaccacgg ccgaggattg gcggtgggca tgtgcaatgg
     3481 aggagtacct gtcatgagtt ccggaaacat gccgcacacc cggcaccccg aattgttcct
     3541 ttggtcggag gcgctggatc ccgtcgacta cgtggtcgca cgctaccgcg tcgcctccct
     3601 gcacgacggc gacgccaccg cgatggcgat ggccatggag cagagcgcgg ccaccaccca
     3661 catcgccggc cacgtggagc cgtcgatgct gcgggactgg accctgcgcg tgcgctcggt
     3721 gcggtcgatc gaatcgctgg gcggcagcgg gctggcgccc taccacctgg ccaccgaggt
     3781 ctatgcagac ggcggcgacg ccgagcgcca ctgggcggtg gaactggcga ttccgcgtcg
     3841 gctgatctcg ggcaagcccg cgcagctgtg gaacgtggtg gtcggcgaga tcccgcggct
     3901 cggcttcctc acccgcttcc agttgtccgg gctcgacctg ccggcggatt tcgggcccgg
     3961 ccccggcttc ggcgtggccg gcatccgccg acagttcggc tgcgagcgcg gcccgctgtt
     4021 gtgccgttcg atgcgcccgg cggtcggcct ggacatcccg accatggccg gcctcaaccg
     4081 ggccgtgctg gaaggcggct tccacctggt caaggacgac gagttgcagg tgttcgcgga
     4141 tgaagccgcg ttccgcgcgc atgtgcaggc gatgctggca gcccgcgatg ccgcgcgcgc
     4201 atcgaccggg cagcgcaagg gctacctcgc caacctgatc tgcgaaccgg acgaactgca
     4261 ggcgcgctgg gagatcgcct gcacgctggg cgtcgatgcg gtgctggtcg cgccgggcat
     4321 ccaggggctg ggcacgctgg ccacgctggc acggcagaag cgcatgccga tcctcgccca
     4381 caacaccttc agcgacctgc tgacacgcca cccggactgg ggcatcgcgc atccggtgct
     4441 gagtggcctg tacgaatcct tcggtgcgga ctggatcgtg acctccggcg aatacggcgc
     4501 cagcgatggc gtggggatgg acgatgcctg gccgttcggc cgtccccagc gggcgatgcc
     4561 gatcctgcag ggcggcaaga gtccggaagg cctgccgacg tttcgcgcgg cgatcggcag
     4621 cgacgattac atgctgatcg tggcgtcgtg ggtggatggc catgccgatg ggctgggcaa
     4681 cgcagcgggc atcttccgcg ccgcggtcga cgcgcagctg cctgcctgag cgacggcaat
     4741 gggcgacgct cgcaccggcg tgtcgttcct gatgctggcg gccggtgcag gcgaacgcct
     4801 cggcctgggc ccgaagggtt cgttggagct gcagggccgg ccgctgttgt gctggctcgc
     4861 ggacaaggcg gtgcaggtcg cggaggacgt gctggtcgcg gtgccggcgg agcacgtgga
     4921 ggcgatcgct gcgctgttgc cgcaatgccg cgtcatcgcc ggtggcgcca cccgccagga
     4981 cagcatcgcg ctgctggcgg cgaaggcaag cggcgagtgg ctgctgctgc acgaggcggc
     5041 ccgtccgttc gccagcctcg cactgttccg ccaggtcatc gcgatggcgc gccatcacgg
     5101 ctgcgccggc gcgttcctgt cgccggtcgt gccggtcgcg cgcctgcgcg atggccgggt
     5161 ggcggaggcg tatgcgggca gcgaggtcgg catcttccag accccgcagg tgtatgcgcg
     5221 cgccgacatg cagcggatgc tgcagcatca ggcaagcgtg ccgggctggt tcgcgcagtc
     5281 gccggtgcag cttgcgctgg cggcaggcgt cgaggtgcat gtcgtgcccg gcgaagcctg
     5341 caacctcaag atcaccaccg ccgacgattg gcggatggcc cgccacctgg cgcatctgtt
     5401 ggcctgaccg cgatcacgcc tcccgcaact gcgggattca atcgggcaaa aggcgcaggg
     5461 acactgcgtt gcgggcgacg ggcccggcga tcgggaagcg agcgatggga aacgtcagga
     5521 caccgatggt cgcattggcg atcgcctgcc tcggcgtgtg cgcggtggct cccgtgcagg
     5581 cgcaggaagc caccgcggtc accctgccgg ccaagcagat ggaagcgtac atgggccgca
     5641 tccagggcaa ggaagcgcag atcgcctatc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nccaatgccg agctgggcgt gccggtcctg aaactggaca acaccgtgaa
     5821 cgcgcaggac ttcgtggtgg tctcgctgcc cggccgcgcg ccggtggata ccaaggcgat
     5881 cagcaacggc atggcgatcc agcgcttcct gaaaggcaag caaggcctca acgccagcat
     5941 cgtctacgaa ttcgcgcccg gcaccgtggc cgagaccgac tcgcgcatgc tcggtacgga
     6001 ggccggcgcc ggcctgtggt tcaacgcgcg cgcagatttc atcggcgccg aacgcggcgg
     6061 ctggggcaac atcaacacca agcccgaagg cctcgtggtc gcgccggaca tcggcacgtt
     6121 cggcgaaatc gccggctcca aggcgggcac cgcggagaac gtgatccgct tcatcggcgg
     6181 catgggcggc gtggaccgca ccggttacca gatgaccccc gactgggcca gggcggaggc
     6241 ggcgatgctc gccgcgggca aggccttcaa cgccgaactg gtggcgcgcc tgaagtaggg
     6301 aggggcctcc aaggccggct atgctgccgg ccttggaggg tttcatgacg acacgaacga
     6361 acgccgatgt cctgatcatc ggtggcggac acaacggcct ggtctgcgcg gcctatctcg
     6421 cggccaccgg cctgaaggtg cgcgtgctcg agcgcaggca cctcgtcggt ggcgcggcgg
     6481 tgaccgagga attccatccc ggcttccgca attcggtggc cagctacacg gtcagcctgc
     6541 tcaacccgaa ggtgatcgcc gacctgcgcc tggcgcagca cggcctgcgg gtagtggagc
     6601 ggccgtacgc gaatttcctg ccgttgccgg atggccgcag cttccgcctc ggcggcgctc
     6661 acaccgacag cgagatcgcg aagttcgcac cgggcgatgt cgcgcgcttg gccgattaca
     6721 ccgcgatgct cgaccgcgtg gtggcggtgc tgaaggaact catccacctc acgccgccca
     6781 acatcggcga acgcatcgcc ttcgccgact ggctgggctc gtatgcggtc gcgaagcgcc
     6841 tgaagcacct cgacctgcgc ggccgccgcg acctgctcga cctgttcacc aagtccgccg
     6901 gcgaattgct ggatcactat ttcgaaggcg agccgctgaa ggccgtactg ggctgggatt
     6961 cggtggtcgg caatttcgcc agcccgtaca cgccgggcag cgcctacgtg ttgctgcacc
     7021 acctgttcgg cgaggtgaac ggcaagaccg gcgtgtgggg ccacgcgatc ggcggcatgg
     7081 gcgcgatcac ccaggccatg cgcaaggaat gcgaggcgcg cggcgtcgcc atcgacaccg
     7141 atgccgaggt cgcgaagctg cttgtcgaaa acgaaagagc gattggtgtc gtgcttgccg
     7201 atggccgcga actgcgcgcg cccaccatcg tcagcaacct caatccgaag ctgctgtaca
     7261 cgaagctggt cgagcgcagg caactggacg acgacaccgc gcagcgcatc gaacgctatc
     7321 gcaatgggtc cggcacgttc cgcatgaatg tcgcgctggg cgagctgccg gacttcaccg
     7381 cgatgcc
//
LOCUS       DAOOCX010000106         7378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_9464_length_7378_cov_7.504848, whole genome shotgun sequence.
ACCESSION   DAOOCX010000106 DAOOCX010000000
VERSION     DAOOCX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9464_length_7378_cov_7.504848"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..4937)
                     /locus_tag="PK399_09550"
     CDS             complement(42..4937)
                     /locus_tag="PK399_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008571584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HPW13403.1"
                     /translation="MVRALRPVLAALLAAALVVCLPACKRNPSGDLPATSGEAIQAKR
                     KEVKGFAVVRAYPDQGRDTLAIAVEFSRPLVGTQDFDKLLTFDAAASNEESSWTLDDD
                     GKILRFPHVEPNKTYTLTIAAALTAADGSKIGRDIEMKVFTGELEPAVGFASQGSVLP
                     AKDSRGLPVVSVNVAEVDVEFLRVREASLPRFFAEYQRGGRRGSWELDPSWYDESDED
                     SRPPISKLAEPVYVNRFVLGGARNERVVTHLPVQDIAELQQPGLYFAVMKKAGAFKDD
                     YDTAFFTVSNIGLHVRAYKDKLFVHTASLADGDPLANAELRVLDARGEPVFKGKTDGN
                     GNALLDYTLDAGHVLTAARGKDVSMLPFNQPALDLSEFAVSGRGQAWFDVFAWSGRDL
                     YRPGETVRVSALLRDNDGKPVVTKNGKAQSLFVRLKQPDGKTFVESRIESGAQGYFRF
                     EKAIPVEAPTGKWQVEFRTDPASKEAVQGMALRIEEFLPERMKLDLDSADPLLHPGEP
                     LRLRATGAYLYGAPASGNRFTAKLAVMVDQHPLAQAKGYFFGDPTVELPKEAKDVVEG
                     TLDAEGKLAQDIALPVEVKGNTPVKAIVSGSLYESGGRSVNRSVERVLWPADALVGVR
                     PLFDDEDGADVNANARFELMRFGPDGTPRPLKGLKVTLVREHRDYHWSHDDDTGWDYD
                     FTRRFENVQVRTLDAGTTAVRFDFPVEWGEYRIDVLDPVTKLTSRYPFHAGWGWDDAN
                     RGLDARPDKVKLALDKTAYRAGDTLKATLTPPHAGKGLLLVESDRMLFVQAIDVKPGT
                     VVEVPVTKEWERHDVYVTALVFRGGSATSKITPARAVGVAFVPMDRRDRKVAVGLAVK
                     PRVRPEQPLQVTVSVPALAGKQAYATVSAVDVGILNITRFPVPDANAHFFAQRRLGVD
                     AYDIYGRVIESFEGGIAKLRFGGDMPLAALPQARRPTARVQTVDLFAGPVTLDAKGNA
                     RVSLAVPDFNGTLRVSALVYSDTQYGNRDAETVVRAPIVAEASMPRVMAPGDRATVTL
                     DITNFTGKPGEFKVRVDGIGPASVGEGSRTLQLAGEAKSTLSFPLAAQEGYSVAQVRV
                     RVDGNGAKVDRKYDLPVRPAWPSVVRSRTQVLGEGAAATMDAGFAEGLMPGSVSARMQ
                     VSAVPPIPFTAALKGALDYPYGCAEQTTSKGYAALLLDAASAKQFGVGGLDAAKRRAR
                     MEGAFGRLASMQVASGHFSMWGSDDYVDPALTPYIVEFLLDAREGGFAVPDAVLQKAL
                     QRLSEDLLAGGHEFYGRDHREYLKFAYQAHAGYALARVNRAPLGTLRALYDNERKKAL
                     KGLPLVQLGLALAMQGDKVRGQKAIAEGFAFAGDRVGYLGDYGSPLRDTALMIALTHE
                     RGYAKPAFDARVIDLGRDLDARRNAPWFYLSTQEQIALARLGKALLADQKKQVAGTLT
                     TAAGNEVIAAAKAFGRDFDFTALASGVRFLPQGEPPFYASLDVAGVPRSAPAADASVI
                     KVERSLFGTDGKPWTPRPLKEGEALIVRVSITADRDMPDALLTDLLPAGLEIENFNLG
                     DGKQWADVVVDGIDITSRGSEAEIRHEEFRDDRYIAALKLSAGSPARLFYLVRAVTPG
                     TYTAPPSLVEDMYRPALRGVV"
     gene            complement(5084..>7378)
                     /gene="gyrA"
                     /locus_tag="PK399_09555"
     CDS             complement(5084..>7378)
                     /gene="gyrA"
                     /locus_tag="PK399_09555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPW13404.1"
                     /translation="ADIDKETVDFQPNYDEKEQEPTVMPTRFPNLLVNGSAGIAVGMA
                     TNIPPHNLNEVVNALLALIDDPSIDIDGLMEHIPGPDFPTAGIINGVGGIHLAYRTGR
                     GRVRMRARAEIEVADNGREAIAVSEIPYQVNKARLIEKIAELVKEKKLEGISELRDES
                     DKDGMRIYIEVKRGESAEVVLNNLYSQTQMESVFGINMVALVDGRPQLLNLKQILQAF
                     VRHRREVVTRRTIFELRKARARAHILEGLTVALANIDEMIELIKTSANPNEARERMLA
                     KTWQPGLVGALLAAAGSDASRPEDLPMGVGLVDGAYQLTEIQAQQILEMRLHRLTGLE
                     QEKLTEEYKLLLDTIRGLIEILEDPDVLLQVIRTELENVKEEFGDARRSEIRASEEDL
                     DILDLIAPEDVVVTLSHAGYAKRQPVSAYRAQRRGGRGRSAAATKDEDFIDQLWLVNT
                     HDTLLTFTSSGKVFWLPVHQLPDAGSNARGRPIINWIPLEAGEKVQAVLPVREYAEGQ
                     YVFFATRDGTVKKTPLTEFAYRLARGKKAINLDEGDALVGVALTDGQRDVLLFASNGK
                     TVRFGEGKVRSTGRTSTGVRGIRMAKGEEVVSLIVAESAGGIELDDDVGNEVAEEEAI
                     ETATDVVEVAIDDGATAYILTATENGYGKRTPLAEYPRKGRGTQGVIGIQTSERNGKL
                     VGAVLLGSTDEVLLISDGGTLVRTRASEISQVGRNTQGVTLIRLSKGEKLQAVERLDG
                     SLVEDVEVSGEAAAVDAIDPPAAP"
BASE COUNT         1113 a         2560 c         2409 g         1296 t
ORIGIN      
        1 gcgcgcctgt tctacctcgt gcgcgcggtc acgccgggca cctataccac gccacgcagt
       61 gcgggtcgat acatgtcctc gaccagcgac ggtggcgcgg tataggtgcc cggcgtgacc
      121 gcgcgcacca ggtagaacag gcgcgccggg ctccccgcac tcagcttcaa ggcggcgatg
      181 tagcggtcgt cgcggaactc ctcgtggcgg atctcggctt cgctgccgcg cgaggtgatg
      241 tcgatgccgt ccaccaccac gtcggcccac tgcttgccgt cgcccaggtt gaaattctcg
      301 atctccaggc cggccggcaa caggtcggtg agcaaggcat ccggcatgtc gcggtcggcg
      361 gtgatgctga cgcggacgat caaggcctcg ccttccttca gtggccgcgg tgtccacggc
      421 ttgccatcgg tgccgaacag gctgcgctcg accttgatca cgctggcatc cgccgccggc
      481 gcgctgcgtg gcaccccggc cacgtccagg ctggcgtaga acggcggctc gccctgcggc
      541 aggaaccgca cgccactggc cagcgcggtg aaatcgaaat cgcgaccgaa ggccttggca
      601 gcggcgatga cttcgttgcc tgccgcagtg gtcagcgtgc cggccacctg cttcttctgg
      661 tcggccagca aggccttgcc gaggcgcgcc agcgcgattt gctcctgcgt gctgaggtag
      721 aaccacggcg cgttgcgacg tgcgtcgagg tcgcgaccga ggtcgatcac cctggcgtcg
      781 aaggcgggct tggcgtaacc acgctcgtgg gtgagcgcga tcatcaacgc ggtgtcgcgc
      841 agcgggctgc cgtagtcgcc gaggtagccc acgcggtcgc ccgcgaacgc gaagccttcg
      901 gcgatcgcct tctgcccgcg caccttgtcg ccctgcatcg ccagcgccag gccaagctgc
      961 accagcggca ggcccttcaa cgccttcttg cgctcgttgt cgtacaacgc gcgcagggtg
     1021 cccagcggcg cgcgattcac ccgtgccaac gcataacccg catgcgcttg gtacgcgaat
     1081 ttcaggtact cgcgatgatc gcggccgtag aactcgtggc cgccggccag caggtcctcg
     1141 ctgagccgtt gcagggcctt ctgcagcacc gcgtcgggaa cggcgaagcc accctcgcgc
     1201 gcgtccagca ggaactcgac gatgtacggc gtcaacgcgg gatcgacgta atcgtcgctg
     1261 ccccacatcg agaaatgccc ggacgcgacc tgcatcgacg ccagccgtcc gaacgcgcct
     1321 tccatccgcg cacgccgctt ggccgcatcc agcccgccca cgccgaactg ctttgcactg
     1381 gcggcatcca gcagcagcgc cgcgtaaccc ttgctggtgg tctgttctgc gcatccatac
     1441 ggatagtcca gcgcgccctt gagcgcggcg gtgaacggga tcggcggcac cgcgctgacc
     1501 tgcatccgcg cgctcacgga gcccggcatc aggccttcag cgaagccggc atccatcgtc
     1561 gcggcagccc cttcgcccag cacctgggtg cgcgaacgca ccaccgacgg ccacgccggt
     1621 cgcaccggca ggtcgtactt gcggtcgacc ttggccccgt tgccatccac ccgcacgcgc
     1681 acctgcgcca cgctataacc ctcttgcgcg gcgagcggga aactcaacgt ggacttggct
     1741 tcgccggcaa gctgcagcgt gcggctgccc tcgccgacgg atgccgggcc gatgccgtcc
     1801 acccgcacct tgaactcgcc gggcttgccc gtgaagttgg tgatgtccag ggtgaccgtg
     1861 gcgcggtcgc ccggcgccat cacccgcggc atgctggcct cggccacgat cggcgcgcgc
     1921 accaccgtct cggcatcgcg attgccgtat tgcgtgtccg agtacaccag cgccgagacc
     1981 cgcagggttc cgttgaaatc cggcaccgcc aacgagaccc gcgcattgcc cttcgcatcg
     2041 agcgtcaccg gtccggcgaa caggtcgacg gtctgcaccc gcgccgtcgg tcgccgcgcc
     2101 tgcggcaatg cggccagcgg catgtcgcca ccgaagcgca gcttggcgat gccgccttcg
     2161 aagctttcga tcacccgtcc atagatgtcg tacgcatcca cgccaagccg gcgctgggcg
     2221 aagaaatgcg cgttcgcatc cggcaccggg aagcgggtga tgttgaggat gccgacatcg
     2281 accgcggaca ccgtcgcata cgcctgcttg ccagcgagcg cgggtacgct gacggtgacc
     2341 tgcaacggct gctcgggccg cacccgtggc ttcaccgcca ggcccaccgc gaccttgcgg
     2401 tcacgccggt ccatcggcac gaaggccacg ccgaccgcgc gtgccggggt gatcttgctg
     2461 gtcgcgctgc cgccgcggaa caccagggcg gtgacataga catcgtggcg ttcccactcc
     2521 ttcgtcaccg ggacttcgac cacggtcccc ggcttcacgt cgatcgcctg cacgaacagc
     2581 atgcggtcgc tttccaccag cagcaggccc ttgcccgcat gcggcggggt gagcgtcgcc
     2641 ttcagcgtgt cgccggcgcg gtacgcggtc ttgtcgagcg ccagcttgac cttgtccgga
     2701 cgcgcgtcga ggccgcggtt ggcatcgtcc caaccccagc cggcgtggaa cggatagcgc
     2761 gacgtcagct tggtcacggg atcgagcacg tcgatgcggt attcgcccca ctcgaccggg
     2821 aaatcgaagc gcaccgcggt cgtgcccgca tccagcgtcc gcacctgcac gttctcgaag
     2881 cggcgggtga agtcgtagtc ccagccggtg tcgtcgtcat ggctccagtg gtaatcgcga
     2941 tgctcgcgca ccagggtcac cttcaggccc ttcagcgggc gcggcgtgcc atcggggccg
     3001 aagcgcatca gctcgaagcg cgcgttggca ttcacgtcgg cgccatcttc gtcatcgaac
     3061 aacgggcgca cgcccaccag cgcatcagcc ggccacagca cgcgttccac gctgcggttg
     3121 acgctgcggc cgccggattc gtacaggctg ccggagacga tcgccttcac cggggtattg
     3181 cccttcactt ccaccggcag ggcgatgtcc tgggccagct tgccttcggc gtccagcgtg
     3241 ccttcgacca cgtccttggc ttccttcggc aattccaccg tggggtcgcc gaagaaatag
     3301 cccttggcct gtgccagcgg atgctggtcc accatgaccg cgagtttggc ggtgaagcgg
     3361 ttgccggatg caggtgcgcc gtagaggtag gcgccggtgg cgcgcaggcg caacggttca
     3421 cccggatgca ggagcggatc cgcgctgtcc aggtccagct tcatgcgttc cggcaggaat
     3481 tcctcgatgc gcagggccat gccctgcacc gcttccttgc tggccgggtc ggtgcggaat
     3541 tcgacctgcc acttgccggt cggcgcttcg accgggatcg ccttctcgaa gcggaaatac
     3601 ccctgtgcgc cggattcgat gcgcgactcg acgaaggtct tgccgtccgg ctgcttgagg
     3661 cgcacgaaca gggactgcgc cttgccgttc ttggtcacca ccggcttgcc gtcgttgtcg
     3721 cgcagcagcg cggagacccg cacggtttcg ccgggacgat agaggtcgcg acccgaccag
     3781 gcgaacacgt cgaaccaggc ctgcccacga ccgctgaccg cgaactcgga caggtccagt
     3841 gccggctggt tgaacggcag catcgacacg tccttgccgc gcgccgcggt gagcacgtgc
     3901 ccggcatcca gggtgtagtc cagcagggcg ttgccattgc cgtcggtctt gcccttgaac
     3961 accggctcgc cgcgcgcatc cagaacccgc aactcggcat tcgccagcgg gtcgccgtcc
     4021 gccagcgagg cggtgtgcac gaagagcttg tccttgtagg cgcgcacgtg caggccgatg
     4081 ttgctgacgg tgaagaacgc ggtgtcgtaa tcgtccttga acgcgccggc cttcttcatc
     4141 accgcgaaat acaggccggg ctgctgcagt tcggcgatgt cctgcaccgg cagatgggtc
     4201 accacgcgct cgttgcgtgc gccgcccagt acgaatcgat tgacgtagac cggctcggcc
     4261 agcttggaga tcggcgggcg actgtcctcg tcgctttcgt cgtaccagga cgggtcgagt
     4321 tcccaactgc cgcgacggcc gccacgctgg tattcggcga agaagcgcgg cagcgaagct
     4381 tcgcgcacgc gcaggaattc gacatcgacc tcggccacgt tgaccgagac caccggcaag
     4441 ccgcgcgaat ccttcgccgg caacacgctg ccctgcgagg cgaagccgac cgccggctcc
     4501 agttcgccgg tgaacacctt catctcgatg tcgcggccga tcttgctgcc gtctgcagcc
     4561 gtcagtgcag cggcgatggt gagggtgtag gtcttgttgg gttcgacgtg cgggaagcgg
     4621 aggatcttgc cgtcgtcgtc cagcgtccaa ctgctttcct cgttgctggc ggccgcgtcg
     4681 aaggtcagca gcttgtcgaa atcctgggtg ccgaccagcg gccgcgagaa ctccactgcg
     4741 atcgccaggg tgtcgcggcc ctggtcggga taggcgcgga cgacggcgaa gcccttgacc
     4801 tccttgcgct tggcctggat cgcctcgccg ctggtcgcgg gcaggtcacc cgacgggttg
     4861 cgtttgcagg ctggcaagca cacgaccagc gctgccgcca gcagcgccgc gagtaccggg
     4921 cgcaaggcgc gcaccatcga catccgttcg gttcgcattt cgcttccatc catgccaggc
     4981 ggtctggagt ataggacgcg gatcggggag gcaagcggcc cacgccggga caaagaaaaa
     5041 gcccggcgtt cagccgggct ctcgtcttcc agggacggtg cgatcagggc gccgcgggtg
     5101 gatcgattgc atccacggca gccgcctcgc cggatacctc cacgtcttcc accagcgagc
     5161 cgtcgaggcg ttcgaccgcc tgcagcttct cgcccttcga cagccggatc agggtcacgc
     5221 cctgggtgtt gcgacccacc tgcgagatct ccgacgcgcg ggtgcgcacc agggtgccgc
     5281 catcggaaat cagcaggact tcatccgtgc tgcccagcag gaccgcgccg accagcttgc
     5341 cgttgcgttc gctggtctgg atgccgatca cgccctgggt gccgcgaccc ttgcgcgggt
     5401 attcggccag cggggtgcgc ttgccgtagc cgttctcggt cgcggtcagg atgtacgcag
     5461 tcgcgccgtc gtcgatggcg acttcgacca cgtcggttgc cgtctcgatg gcctcctcct
     5521 cagccacctc gttgccgaca tcgtcgtcca gctcgatccc gccggcggac tccgccacga
     5581 tgaggctgac cacttcctcg cccttggcca tgcggatgcc gcgcacgccg gtcgaggtgc
     5641 ggccggtcga acgcaccttg ccctcgccga agcgcacggt cttgccgttg ctggcgaaca
     5701 gcagcacgtc gcgctggcca tcggtcaggg ccaccccgac cagggcatca ccctcgtcca
     5761 ggttgatcgc cttcttgccg cgcgccaggc ggtacgcgaa ttcggtgagc ggggtcttct
     5821 tgaccgtgcc atcgcgggtg gcgaagaaca cgtattggcc ttcggcgtat tcgcgcaccg
     5881 gcagcacggc ctgcactttc tcgccggctt ccagcggaat ccagttgatg atcgggcgac
     5941 cacgcgcatt ggaaccggcg tcgggcaact ggtgcaccgg cagccagaac accttgccgc
     6001 tgctggtgaa ggtcagcagc gtgtcatgcg tgttgaccag ccacaactgg tcgatgaaat
     6061 cctcgtcctt ggtggccgcc gcactgcgcc cgcgaccgcc acggcgctgc gcgcgatacg
     6121 cgctgaccgg ctggcgcttg gcatagccgg catgcgacag cgtgaccacc acgtcttccg
     6181 gcgcgatcag gtcgaggatg tcgaggtctt cttccgacgc gcggatttcg ctgcggcggg
     6241 cgtcgccgaa ctcttccttg acgttctcca gctcggtgcg gatcacctgc agcagcacgt
     6301 ccggatcctc gaggatctcg atcaggccac ggatggtgtc cagcagcagc ttgtattcct
     6361 cggtcagctt ctcctgctcc agtccggtca ggcggtgcag gcgcatctcc aggatctgct
     6421 gcgcctggat ctcggtgagc tggtacgccc catcgaccag gcccacgccc atcggcaggt
     6481 cttcgggacg cgaggcatcg ctgcccgcgg cggcgagcag cgcgccgacc aggcccggct
     6541 gccaggtctt ggccagcatg cgctcgcgcg cttcgttcgg attcgccgag gtcttgatca
     6601 gctcgatcat ctcgtcgatg ttggccagcg cgacggtcag gccttccagg atgtgcgcgc
     6661 gggcgcgggc cttgcgcaac tcgaagatgg tgcgacgggt cacgacttcg cggcggtggc
     6721 ggacgaaggc ctgcaggatc tgcttgaggt tcagcagctg cgggcggcca tcgaccagcg
     6781 ccaccatgtt gatgccgaac accgactcca tctgggtctg cgaatagagg ttgttgagca
     6841 cgacctccgc ggactcgccg cgcttgacct cgatgtagat gcgcatgccg tccttgtcgg
     6901 actcgtcgcg caactcgctg atgccctcga gtttcttctc cttcaccagc tcggcgatct
     6961 tctcgatcag ccgtgccttg ttgacctggt acgggatctc gctgaccgcg atcgcctcgc
     7021 ggccgttgtc ggccacttcg atctccgcgc gggcgcgcat gcgcacgcgg ccgcgaccgg
     7081 tgcgataggc caggtggatg ccgcccacgc cattgatgat gccggcggtg gggaagtccg
     7141 ggccggggat gtgttccatc aggccgtcga tgtcgatcga cggatcatcg atcagcgcca
     7201 gcagcgcgtt cacgacttcg ttgaggttgt gcggcgggat attggtggcc atgcccaccg
     7261 cgatgccggc ggacccgttg accagcaggt tcgggaaccg ggtcggcatg accgtgggtt
     7321 cctgttcctt ctcgtcgtag ttcggctgga aatcgacggt ttccttgtcg atgtcggc
//
LOCUS       DAOOCX010000107         7205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_9725_length_7205_cov_8.680839, whole genome shotgun sequence.
ACCESSION   DAOOCX010000107 DAOOCX010000000
VERSION     DAOOCX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9725_length_7205_cov_8.680839"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..427
                     /gene="queD"
                     /locus_tag="PK399_09560"
     CDS             68..427
                     /gene="queD"
                     /locus_tag="PK399_09560"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPW13405.1"
                     /translation="MDIFKVFTIEAAHRLPNVPEGHKCARVHGHSFRIELHASGEPDP
                     HLGWIMDFSELKAAFQPLYDQLDHHYLNDVEGLANPTSERLATWIWERLKPLVPLLSE
                     VVVHETCTSGCRYRGPG"
     gene            502..705
                     /locus_tag="PK399_09565"
     CDS             502..705
                     /locus_tag="PK399_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPW13406.1"
                     /translation="MYVCICNGVTEQQVRDAAEAGCASVAELTMRTGAGANCGSCIGL
                     AADLLEEHRARRALPLPILAQAA"
     gene            863..1336
                     /gene="bfr"
                     /locus_tag="PK399_09570"
     CDS             863..1336
                     /gene="bfr"
                     /locus_tag="PK399_09570"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HPW13407.1"
                     /translation="MKGDAKVIEFLNKALYNELTAINQYFLHAKMLKNWGLKELAEHE
                     YHESIDEMKHADALSERILFLDGLPNFQALGRLRIGENPLEVLRADLALELEAIPLLR
                     EAIAHCEAVNDFGSRKLFSDILVSEEEHVDWIETQLSLIERIGEANYLAQQLDKG"
     gene            complement(1349..3616)
                     /locus_tag="PK399_09575"
     CDS             complement(1349..3616)
                     /locus_tag="PK399_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW13408.1"
                     /translation="MTSGDERGFNLLTARWLALGAAAGATLSLLAAQLGVQGPWQGTG
                     LVLAFLALFGSTAMAFAEHRRERELHDQTDLNRRGAAEISALQQEIDRHTQLEDELTK
                     AKQAAESAVMAKGEFLATMSHEIRTPLNGIVPMLDLLSHSRLAPDQHELLRTATASAQ
                     QLLRIVDDILDYSKLEADRLDLETTGFNLRDLMQSVLTLMERTAEAKGLRLVLNIDPS
                     VRLPVRGDPVRLRQILSNLISNAVKFTERGSITLHVKRLRETAAQHQLRFEVQDTGIG
                     IEPAAQEKLFRAFTQADASTTRLYGGTGLGLAICRRIVTLMGGRIGLESELGHGSTFW
                     FEIPLLKVQGDMPLQPEELHGGRVLLITTDQRLRMRLTLLLPNWGLRVTAVENTHDAL
                     ERLRQASSQGEPWSYSVVLADLGSIRSTAVSLSRNMERKNQYGDARLVYLRGDDATSL
                     DLPVSAIVIPRNAEDADLRSVLSASGVSWARHDQHPAPAAKAPPPDPERKAKILLVED
                     NPVNLMVAQRLLQVLGAECDTAGNGQVALEKLEAGGFDMVLMDCQMPVLDGYSATRRW
                     RELEQQRNADHRVPIVAMTANAMAGDRQKCLDAGMDDYLAKPVTRNDLERCIERWRGV
                     SMVVPATLPPIEQIAARSPVVINASVLDELREVLGGEVDKIVAVYLEDAPRLIAQLER
                     AAVGNDPIALRVAAHTLKSSSANVGATTLSDAARDLEHGARDGTLTQPATAVARIVGE
                     FAQVRAALQAQLDKT"
     gene            complement(3613..5307)
                     /locus_tag="PK399_09580"
     CDS             complement(3613..5307)
                     /locus_tag="PK399_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW13409.1"
                     /translation="MSAEPATENDKTDPYRVVIVEDDRSQALFAEAILRGAGMLAEVV
                     SAPERLMATMEQFAPDLVLMDLHMPGTSGTTLTVQIREHPRFEQIPVVFLTGDQDPDR
                     QLEVLELGADDFILKPVRPRHLIAAVQSRVRRARAAQNRQVVAEPEHHPVTGLFTRAA
                     LMQKLGASIPSTTGGALLVEMGNAVALRNRYGYAGFESLMNDAGRLLGTITKGKAAAR
                     LSDNAFLVLAPDLQPGQLDAYARTLRDGIGYHDFHVEQEPLRLRCAIGYAALSHGFAD
                     GGAVLAAAEDAVRIAREQVIGIAAYVPPPDVAPPTGGIADALRGSLSGEADLIHLAFQ
                     PVVAVAGGEDAQFQVLVRMRDAKGVEQMARDFLPVAEASGLLPALDRWVMERALVLLQ
                     KRRAESKPMRLFVSQSPRTLAQDAYSGWLVHALEQASVEGTSLVVDIRLDDALVHSML
                     LRQFCERMVPAGVQFCLSQYRHGADADMLLQQLPLGYLRLAADFARQPLPPELRDEMR
                     EVIDRAHRLGLQVIGQAVEDPQAAAALWMGGIDFIQGNLVQRAEQALDFDFQHATL"
     gene            complement(5402..5983)
                     /locus_tag="PK399_09585"
     CDS             complement(5402..5983)
                     /locus_tag="PK399_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HPW13410.1"
                     /translation="MEQSHLFAIGVLLAWLAGVRVYLTVFGVGLAGALGWLQLPDALQ
                     ATQSAWVLGTAGSLAAAEFFADKIPGVDSIWDLLHTVLRVPVGAFLAAAAMSPDGELG
                     AGMLATGAGVALTSHVIKAGSRALLNTSPEPVSNWTASVTEDAAVVGGLALAFTHPWL
                     ALAIVVAISLLFAFSVWWLWRRLFRRAPRPELA"
     gene            6085..6801
                     /locus_tag="PK399_09590"
     CDS             6085..6801
                     /locus_tag="PK399_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13411.1"
                     /translation="MSTPHAPPHQRDTGHPRMALLLLVLASVAFGGWGAWRSFGPQPA
                     NAGERLRDQGDRIEELEQQVATLTRSDQISRQANADLQGTLAERDEEIASLRADIAFY
                     ERFVGATAQRHGLSLHELVLEPQRDPQLWHFVATLTQNLNRGAVSTGKLQLSVEASED
                     GRMRKLAWSELRQQQNAPGVDYSFKYFQQVEGDIVLPPGVKPVRLIARLVPASGPPVE
                     QSLTWGEATSPAGATVPATR"
     gene            6910..>7205
                     /gene="erpA"
                     /locus_tag="PK399_09595"
     CDS             6910..>7205
                     /gene="erpA"
                     /locus_tag="PK399_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HPW13412.1"
                     /translation="MQFTAAAAAKVRELIAEEGNADLKLRVYIQGGGCSGFQYGFEFD
                     EGQNEDDLPIVTDGVTLLVDPLSLQYLMDATVDYTESLTGAQFVIRNPNAKTTCG"
BASE COUNT         1179 a         2442 c         2449 g         1135 t
ORIGIN      
        1 caccacggcg cagcacgcaa ccccgcttcc ggcggggttt ttcgtttctc ttcccgcgga
       61 tgccggcatg gacatcttca aggtcttcac catcgaggcc gcgcaccggc tgcccaacgt
      121 gcccgaaggc cacaagtgcg cgcgcgtgca cgggcattcg ttccggatcg agttgcacgc
      181 ctccggcgaa ccggatccgc acctgggctg gatcatggat ttctccgagc tgaaggccgc
      241 gttccagccg ctctacgacc agttggacca ccattacctc aacgatgtcg aggggttggc
      301 caacccgacc agcgaacgcc tggccacgtg gatctgggag cggctgaagc cgctggtgcc
      361 tttgctgtcg gaggtcgtgg tacacgaaac ctgcacgtcg ggttgtcgct atcgtggccc
      421 gggctgagcg tcgaaaccct aacgattgac aatcattcgc gtttgagatc aaatcgcgtc
      481 ctcgatcttg cgagtggtgc gatgtacgtc tgcatctgca atggcgtcac cgaacagcag
      541 gtccgcgatg ccgcggaggc cggttgcgca tcggtggccg aactgaccat gcgcaccggt
      601 gccggcgcca actgcggttc ctgcatcggc ctggctgccg acctgctgga ggaacaccgg
      661 gcgcgacgcg ccttgccgtt gcccatcctg gcccaggccg cctgatccga ctcgcagccg
      721 cgtgctccgc gtttgccatc gatccgcgaa gaggcaagtg ggtgccagcg tgacgcgagg
      781 catgatgcgg acgattcgcg tttcgccgcc gatcgggtgt aaccgctaga ttgccagcac
      841 tccccgcaca cggactgcag ccatgaaggg cgacgccaag gtcatcgaat tcctcaacaa
      901 ggcgctctac aacgagctga ccgccatcaa ccagtacttc ctgcacgcca agatgctgaa
      961 gaactgggga ttgaaggaac tcgccgaaca cgaataccac gagtcgatcg acgagatgaa
     1021 gcatgcggac gccctgtccg agcgcatcct gttcctcgac ggcctgccga acttccaggc
     1081 gctgggtcgc ctgcgcatcg gcgagaaccc gctggaagtc ctgcgcgccg acctggcgct
     1141 ggaactggag gcgatcccgc tcctgcgcga ggcgatcgcg cattgcgaag ccgtcaacga
     1201 tttcggcagc cgcaagctgt tctccgacat cctggtgtcg gaggaagaac acgtcgactg
     1261 gatcgagacc caactgtcgc tgatcgagcg gatcggcgaa gcgaattacc tggcgcagca
     1321 gctcgacaag ggctgatcgc gccaggcgtc aggtcttgtc gagctgcgcc tgcagcgcgg
     1381 cgcggacctg ggcgaattcg ccgacgatgc gcgccactgc ggtcgccggc tgggtcaggg
     1441 tgccatcgcg ggcaccgtgt tccaggtcgc gcgcggcatc ggacagcgtg gtcgcgccga
     1501 cattcgcact ggacgacttg agcgtgtgcg ccgccacccg caacgcgatc gggtcgttgc
     1561 ccaccgccgc gcgttccagt tgggcgatca ggcgcggtgc gtcttccagg tagacggcga
     1621 cgatcttgtc gacttcgccg ccgagaactt cgcgcaattc atcgagcacg gacgcattga
     1681 tcaccaccgg cgagcgggcg gcgatctgct cgatcggcgg cagggtcgcc ggtaccacca
     1741 tgctgacccc gcgccagcgt tcgatgcagc gctccaggtc gttgcgggtg accggcttgg
     1801 ccaggtagtc gtccatgccg gcgtccaggc acttctggcg gtcgccggcc atcgcgttgg
     1861 cggtcatcgc gacgatcggc acgcggtggt cggcgttgcg ctgttgttcc agctcgcgcc
     1921 agcggcgggt ggcgctgtag ccgtcgagca ccggcatctg gcagtccatc aggaccatgt
     1981 cgaagccgcc ggcctccagt ttttccagtg cgacctggcc gttgccggcg gtgtcgcatt
     2041 cggcgcccag gacctgcagc aggcgctgcg cgaccatcag gttcaccggg ttgtcttcga
     2101 ccaggaggat cttcgccttg cgttccggat ccggcggggg cgccttcgcc gcgggcgcgg
     2161 gatgttggtc gtggcgcgcc caggacacgc cggaggccga cagtaccgaa cgcaggtcgg
     2221 cgtcctcggc attgcgcggg atcacgatcg ccgagaccgg caggtcaagc gaggtggcat
     2281 cgtcgccgcg caggtagacc aggcgcgcgt cgccgtactg gttcttgcgc tccatgttgc
     2341 gggacagcga cacggcggta ctgcggatgc tgcccaggtc ggccagcacc accgaatacg
     2401 accacggctc gccctgcgag gacgcctggc gcaggcgttc cagcgcatcg tgggtgttct
     2461 ccacggcagt cacccgcagc ccccagttgg gcaggagcag ggtcaggcgc atgcgcaggc
     2521 gctggtcggt ggtgatcagc aatacgcggc cgccatgcaa ctcttcaggc tgcaagggca
     2581 tgtcgccctg caccttgagc agcgggatct cgaaccagaa ggtggagccg tggcccaatt
     2641 cggactccag cccgatccgt ccgcccatca gggtcacgat gcgccggcag atggccaggc
     2701 ccagaccggt cccgccgtag agccgcgtgg tggaggcatc cgcctgggtg aaggcgcgga
     2761 agagcttttc ctgggcggcg ggttcgatgc cgatgccggt gtcctgcact tcgaagcgca
     2821 actggtgctg ggccgcggtt tcgcgcaggc gcttgacatg cagggtgatg ctgccgcgct
     2881 cggtgaactt gaccgcgttg ctgatcaggt tgctcaggat ctgccgcagc cggaccgggt
     2941 cgccgcgcac cggcaggcgc accgacggat cgatgttgag caccaggcgc aggcccttgg
     3001 cctcggcggt ccgctccatc agggtcagca cgctctgcat caggtcgcgc aggttgaagc
     3061 ctgtggtttc caggtcgagc cggtcggctt ccagcttgga gtagtccagg atgtcgtcga
     3121 cgatccgcag caactgctgg gcggaggcgg tggcggtgcg cagcagctcg tgctggtccg
     3181 gggccagccg cgaatgcgag agcaggtcga gcatcggcac gatgccgttg agcggggtgc
     3241 ggatctcgtg gctcatggtg gccaggaatt cccccttggc catcaccgcc gattccgctg
     3301 cctgcttggc ctttgtcagt tcgtcctcga gctgggtatg gcggtcgatc tcctgctgca
     3361 gggcactgat ctctgccgca ccgcggcggt tgaggtcggt ctggtcatgc agttcgcgtt
     3421 cgcggcggtg ttccgcgaag gccatcgcgg tgctgccgaa cagggcgagg aaggccagca
     3481 ccaggccggt gccttgccac gggccctgca cgccgagctg tgcggcgagc agggacaggg
     3541 tggcacccgc agccgcgccg agcgcaagcc agcgggcggt gaggaggttg aaaccgcgtt
     3601 cgtcgccgga agtcacaggg ttgcgtgctg gaagtcgaag tcgagggcct gctcggcgcg
     3661 ttgtaccaga ttgccctgga tgaaatcgat gccgcccatc cacagcgcgg ccgcggcctg
     3721 cggatcctcc accgcctggc cgatcacttg caggccgagc cggtgcgcgc ggtcgatgac
     3781 ttcgcgcatc tcgtcgcgca gttccggcgg cagcggctgg cgcgcgaaat cggcggccag
     3841 gcgcaggtag cccagcggca attgctgcag cagcatgtcg gcatccgcgc cgtggcggta
     3901 ctggctcagg cagaactgca cgccagcggg caccatgcgt tcgcagaact ggcgcagcag
     3961 catcgaatgc accagcgcgt cgtccaggcg gatgtccacc accagcgacg tgccctcgac
     4021 gctggcctgt tccagtgcat gcaccagcca gcccgagtag gcgtcctgcg cgagggtgcg
     4081 cggcgactgc gaaacgaaca ggcgcatcgg cttgctctcg gcgcggcgct tctgcaacag
     4141 caccagcgcg cgctccatga cccagcggtc cagggcgggc aacagcccgg atgcttccgc
     4201 gacgggcagg aagtcgcggg ccatctgctc cacgcccttg gcatcccgca tccgcaccag
     4261 tacctggaac tgtgcgtcct cgccgcctgc caccgcgacc accggctgga aggccaggtg
     4321 gatgaggtcg gcctcgccgg acagcgatcc acgcagggca tcggcgatcc cgccagtagg
     4381 tggggcaacg tccggcggcg gcacgtaggc ggcgatgccg atcacctgct cacgcgcgat
     4441 gcgcacggcg tcctcggcgg cggccaggac cgcgccgccg tcggcgaagc cgtgcgacaa
     4501 cgcggcatag ccgatcgcgc aacgcagccg cagcggttcc tgctcgacgt ggaaatcgtg
     4561 gtagccgata ccgtcgcgca gggtgcgcgc ataggcatcg agctggccgg gctgcaggtc
     4621 cggcgccagc accaggaacg cgttgtcgct gaggcgcgcc gcggccttgc ccttggtgat
     4681 ggtgcccagc aggcgcccgg catcgttcat cagcgattcg aagccggcgt aaccgtagcg
     4741 gttgcgcagg gcgaccgcat tacccatctc gaccagcagg gcaccgccgg tagtggacgg
     4801 gatgctggca ccgagcttct gcatcaacgc cgcacgggtg aacagtccgg tgaccggatg
     4861 gtgttcgggc tcggccacga cctggcggtt ctgcgcggcg cgcgcgcggc gcacgcggct
     4921 ctgcacggca gcgatcaggt ggcgcgggcg caccggcttg aggatgaagt cgtcggcgcc
     4981 gagttcaagc acctccaact ggcgatccgg atcctggtcg ccggtgagga acaccaccgg
     5041 gatctgctcg aagcgtggat gttcgcgaat ctgcacggtg agggtggtgc cgctggtgcc
     5101 gggcatgtgc aggtccatca acaccaggtc gggcgcgaac tgttccatcg tcgccatcag
     5161 ccgttcgggc gccgatacca cctccgccag catcccggcg ccacgcagga tcgcctcagc
     5221 gaacagcgcc tggctgcggt catcctcgac gatcacgacc cggtacggat cggtcttgtc
     5281 gttttcggtc gccggttcgg cggacatggt catggcttac ccctggagat gcccttgcaa
     5341 gcggcatccg tcgaacgatt ctgacgaaaa aagcgttggc tggaggtcat cgtgccagcg
     5401 ctcaagcgag ttccggccgc ggtgcgcgac ggaacagccg gcgccacaac caccatacgg
     5461 aaaaggcgaa cagcaggctg atcgccacta cgatcgccaa ggctaaccaa ggatgggtga
     5521 aggccagggc gaggccgccg accaccgcgg cgtcctcggt caccgatgcg gtccagttgc
     5581 tgaccggctc cggcgaggtg ttgagcaggg cgcgcgagcc ggccttgatg acgtggctgg
     5641 tcagtgccac gccggcgccg gtggccagca tgccggcccc gagttctccg tcgggcgaca
     5701 tcgccgccgc ggccaggaat gcgcccaccg gcacccgcag gacggtgtgc agcaggtccc
     5761 agatcgaatc cacgccgggg atcttgtcgg cgaagaactc cgccgcggcc agggagcccg
     5821 ccgtccccag gacccaggcc gattgggtgg cctgcagcgc atccggcaat tgcagccagc
     5881 cgagcgcgcc ggccaggccc acgccgaaca cggtcaggta cacgcgcacg cctgccagcc
     5941 aggccagcaa cacgccgatg gcgaacagat gcgattgttc cagctccatc ccgcagccca
     6001 ggcgatgccc cgtggcaccc tccaccgcag tctatacggc gctgcggctt gacgcaggct
     6061 cggacgcggt gcgataccac gcccatgtcg acaccgcatg ccccgccgca ccagcgcgac
     6121 accggccatc cacgcatggc cctgctgctg ctggtgctgg cgtcggtggc gttcggcggt
     6181 tggggcgcgt ggcgcagttt cggcccgcaa cccgcgaatg ccggcgagcg gctgcgcgac
     6241 cagggcgacc ggatcgagga actggagcag caggtcgcca ccctgacccg ctccgaccag
     6301 atcagccggc aagccaatgc cgacctgcag ggcacgctgg ccgagcgcga tgaggaaatc
     6361 gccagcctgc gcgcggacat cgccttctac gagcgcttcg tcggggccac cgcgcagcgc
     6421 cacggcctga gcctccacga actcgtgctg gagccgcaac gcgacccgca gctctggcat
     6481 ttcgtcgcca ccctcaccca gaacctgaac cgtggcgcgg tcagcaccgg caagctgcaa
     6541 ctgtccgtgg aggccagcga ggacggacgc atgcgcaagc tggcctggag cgagctgcgc
     6601 cagcaacaga acgcaccggg cgtcgattac tcgttcaagt atttccagca ggtggagggc
     6661 gacatcgtcc tgccgccggg ggtcaagccg gtgcgcctga tcgcgcgcct cgtgccggca
     6721 tccggtccgc cggtggagca gagcctgacc tggggcgagg cgacatcgcc tgctggcgcc
     6781 acggtgcccg ccacgcgctg atccacggtt gaatcgcgcg gcctcgcgcc ccatcctgac
     6841 cggatggaac agtccctgca cccgctgccc ggcgtcctcg ccgccccgac cacgccgccg
     6901 accacgccga tgcagttcac cgccgcggcc gcggcgaagg tgcgcgaact gatcgccgag
     6961 gaaggcaatg ccgacctcaa gttgcgcgtc tacatccagg gcggcggctg ctcgggcttc
     7021 cagtacggct tcgagttcga cgagggccag aacgaggacg acctgcccat cgtcaccgat
     7081 ggcgtgaccc tgctggtcga tccgctcagc ctgcagtacc tgatggacgc caccgtcgat
     7141 tacaccgagt cgctgaccgg cgcgcagttc gtgatccgca acccgaacgc gaagaccacc
     7201 tgcgg
//
LOCUS       DAOOCX010000108         7183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_9754_length_7183_cov_8.273990, whole genome shotgun sequence.
ACCESSION   DAOOCX010000108 DAOOCX010000000
VERSION     DAOOCX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9754_length_7183_cov_8.273990"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /gene="tadA"
                     /locus_tag="PK399_09600"
     CDS             <1..387
                     /gene="tadA"
                     /locus_tag="PK399_09600"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008577150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPW13413.1"
                     /translation="ALVVDAEGHVVGEGWNRNIAEHDPSAHAEIVAMRRAGAAIGNHR
                     LVGCTLYVTLEPCAMCAMAMVHARVKRVVYGAADPKTGAAGSVFDLLGDARHNHRVEI
                     VGGVLGDQAGTMLTNYFRAKRAKPAI"
     gene            complement(384..845)
                     /locus_tag="PK399_09605"
     CDS             complement(384..845)
                     /locus_tag="PK399_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HPW13414.1"
                     /translation="MLQNTGALSHYAILTAMLAPAFFLTATAALLASANTRLARVVDR
                     LRSLIVAWELDAPDRAERDEQILRHRQRAHLVLRACRLLYGAVASFVGTSLSLAMDAF
                     LGFRLGVVPTLLAVFGVLFLLGASFAMWREVSLAVVSFDAELDQELARRRN"
     gene            904..1473
                     /gene="orn"
                     /locus_tag="PK399_09610"
     CDS             904..1473
                     /gene="orn"
                     /locus_tag="PK399_09610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HPW13415.1"
                     /translation="MNADSHADRLIWIDLEMTGLDTDRDGILEIATVVTDGDLNVLAE
                     GPECAIAHPLATLEAMDDWNRTQHGKSGLWRRVLDEGVALAEAEARTLDFLREWVPAG
                     ASPMCGNSICQDRRFLHRLMPALERHFHYRNLDVSTVKELARRWSPEVLASVRKASAH
                     TALSDVRDSIAELRHYRRYMGALSGLPAA"
     gene            1495..4473
                     /locus_tag="PK399_09615"
     CDS             1495..4473
                     /locus_tag="PK399_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPW13416.1"
                     /translation="MPMASVQGWRGKALVGLLALLCLLPLLAAAQDKPLSAFYRETWT
                     TRQGLPHNQVNAIAQSPDGYLWLGTWEGLVRYNGLEFHVFDRANTPELKDNGIRSVRA
                     ASDGAVVIGTSRGGVTVKRGDRWRTWMEKDGLAQEEVMDALLDRQGRLWVATESAGIT
                     RMDGEVPVQFNERNGLPSDVVYGLLLDRDGSVWAATANGVAHFAKGRIRNYTQQAGLP
                     AAPIFRVMQDASGTLFVGTERGVYRMQGERFVLQSPLLPEDGVPSLARDTAGNLWVGT
                     VNNGLVRLGESGAERFTSVRGLPNNRVAALLVDREGSIWAGTNAGLLRLSDASFSTWN
                     GDQGLSDDYVRALYEAPRGGMWIGTSRGLNLWRDGRIEATYSAADGLPSDSVLSLLES
                     RDGSLLVGSYTDGVLRMRDGKVVGHYDNARGMPGSNQVRALAEEADGTLWIGTSRGLA
                     RYRDGTFKAYGLAEGLPREFIISLHIARDGSLWVGTANGAANIVAGKVRSLDMRPVNG
                     AQDVFAFHEDADGTLWLATDRGLLRYRNGVLRGLGLKEGLPVDTMFSVVDDERGHLWM
                     TSNRGVLRIKRSDAEAVLEGRLAQLPYDHFGEADGLVSAQCNGGSGPAALRDSRGNIW
                     VATSRGAAVTNPARLQAYRHQLPQVVIEQAYADDKPVSAEDGLLRLPPGTRKLEFRYA
                     ALSFLMPRFLRYRYRLEGEDAAWIERGNLRVAQYTNLRPGRYRFLVDVSAPTVGRDWS
                     GEPTVIDIEIKPQPWQQAWFLAVATLLLGLLLFAIYRWRLGNLRKRATELEDVIDQRT
                     SALREQTDRLMESDREKSILLARLKEQSEAFERMALEDALTGIGNRRHFDARLSHAFD
                     RAVETRQPLSFALFDVDEFKRINDQYSHAAGDHALVAVAHALQAGIEGRGSIARWGGE
                     EFAVLFEGMPLDEARQLCEQLRARVQAIDCTEFAPGWSMTISAGVTERTGLAHHEKLV
                     SRADALLYEAKRAGRNRICG"
     gene            complement(4522..6888)
                     /gene="ppsA"
                     /locus_tag="PK399_09620"
     CDS             complement(4522..6888)
                     /gene="ppsA"
                     /locus_tag="PK399_09620"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HPW13417.1"
                     /translation="MNNVLWLNDLRLTDLAQVGGKNSSLGEMIGELSGLGVSVPGGFA
                     TTADAFKAFIAHNDLHARIYDKLATVDVEDVPALTRAGAEIRGWVIDAPLQPELDADI
                     RSAYAKLCADAGSNDIAVAVRSSATAEDLPDASFAGQQETFLNVTGADDVVHKVKEVF
                     ASLYNDRAIAYRVHHGFAHEDVFLSAGVQLMVRSDVGSSGVLFTLDTESGFRDVVFVT
                     ASYGLGEMVVQGAVNPDEFYVYKPTLNAGKPAILRRSLGSKQLRMVYSDAPGERVRTE
                     ETPAALRSTFSISDADVQELAKQSLVIERHYGRPMDIEWGKDGHTGKLYILQARPETV
                     KSRAHATQIERYTLGERGDVLAEGRAIGSKIGSGTARVVRSLADMERVQPGDVLVADM
                     TDPDWEPVMKRSAAIVTNRGGRTCHAAIIARELGVPAVVGTGNALDSVKDGDPITVSC
                     AEGDTGFIYAGALPFERTTTDLGNMPPAPLKIMMNVANPDRAFDFGMLPNAGIGLARL
                     EMIIASHIGVHPKALLEYDRQDAATKAKIDARTAGYAGPVEFYVDRLAEGIATITASV
                     APNPVIVRLSDFKSNEYANLIGGSKYEPHEENPMIGFRGASRYIDPSFKDAFALECRA
                     VKRVREDMGLDNLWVMIPFVRTLGEGRKVIDVLRENGLVQGENGLKVIMMCEVPSNAL
                     MADEFLELFDGFSIGSNDMTQLTLGLDRDSAIVAGLFDERDPAVKKMLSMAISAAKAK
                     GKYIGICGQGPSDHPDLALWLMEQGIESVSLNPDTVVDTWLQLAKAKG"
     gene            6995..>7183
                     /locus_tag="PK399_09625"
     CDS             6995..>7183
                     /locus_tag="PK399_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HPW13418.1"
                     /translation="MDHPRPVFYVSDGTGITAETIGHSMLTQFSGMRFQTDRLPFVDT
                     PEKARLVAARIRAAGEASG"
BASE COUNT         1166 a         2385 c         2490 g         1142 t
ORIGIN      
        1 gcgctggtcg tcgatgcaga agggcatgtc gtcggcgaag gctggaaccg caacatcgcc
       61 gaacacgatc cgtcggcgca cgcggagatc gtggcgatgc gccgcgcggg tgccgcgatc
      121 ggcaatcacc gtctggtcgg ctgcaccttg tacgtgacgc tggagccgtg tgcgatgtgc
      181 gcgatggcga tggtgcatgc gcgcgtgaaa cgggtcgtgt atggcgccgc ggatccgaag
      241 accggcgcgg cgggcagcgt gttcgacctg ctcggcgatg cgcgccacaa ccatcgcgtc
      301 gaaatcgtcg gcggcgtgct gggcgatcag gccggtacga tgttgacgaa ctactttcga
      361 gcgaagcggg cgaagcctgc gatctagttg cgccgccgcg ccagttcctg gtccagttcg
      421 gcatcgaagc tgacgaccgc caggctcact tctcgccaca tcgcgaagct tgcaccgagc
      481 aggaacagca cgccgaacac ggccagcagc gtggggacca cgcccagccg gaagccgagg
      541 aacgcatcca tcgccagcga caggctggtg ccgacgaaac tggccacggc gccgtacagc
      601 aggcggcagg cacgcaggac gagatgggca cgttggcggt gacggaggat ctgctcgtcc
      661 cgttcggcac gatccggcgc atccagctcc caggccacga tcaggctgcg caagcgatcc
      721 accacgcggg ccaggcgcgt gttcgcggac gccagcaacg cggcggtggc ggtgaggaag
      781 aaggcggggg ccagcatcgc ggtgaggatg gcgtagtggc tcagcgcgcc ggtgttctgg
      841 agcatgcagg ttctccgggg gcgagccggt atcatgcccg ctgcccacca cgcttgcgag
      901 cgcatgaacg cggattccca cgctgatcgc ctgatctgga tcgacctcga gatgaccggc
      961 ctggacaccg accgcgacgg catcctcgag atcgccaccg tcgtcaccga cggcgacctc
     1021 aacgtgctcg ccgaggggcc ggaatgcgcc atcgcccatc cgttggccac gctcgaggcg
     1081 atggacgact ggaaccgcac ccagcacggc aagtccggcc tgtggcgacg ggtgctggac
     1141 gagggcgtgg cgctggccga agcggaggcg cgcaccctgg acttcctgcg cgaatgggtg
     1201 cccgccggtg catcgccgat gtgcggcaat tcaatctgcc aggaccggcg cttcctgcac
     1261 cggctgatgc ccgcgctgga acgccacttc cactaccgca acctggacgt gagcacggtg
     1321 aaggaactcg cccggcgctg gtcgccggag gtgctggcca gcgtgcgcaa ggcatcggcg
     1381 cacaccgcgc tcagcgacgt gcgcgactcg atcgcggaat tgcggcacta tcgtcgctac
     1441 atgggggcgc tgtccgggtt gccggcggcg taaagcgggg tcgggatggg gcggatgccg
     1501 atggcgtctg tgcagggatg gcggggcaag gcgctggtcg gcctgctggc gctgctttgc
     1561 ctgttgccgc tcttggccgc tgcgcaggac aagccgctct cggcgttcta ccgggagacc
     1621 tggacgactc gccagggcct gccgcacaac caggtcaacg cgatcgcgca atccccggac
     1681 ggctacctgt ggctggggac ctgggaaggc ttggtccgct acaacggcct tgaattccat
     1741 gtcttcgacc gagccaacac ccccgagctg aaggacaacg gcatccgctc ggtgcgtgcc
     1801 gcctccgacg gtgcggtggt gatcggcacc tcccgcggcg gggtgacggt caagcgcggg
     1861 gatcgttggc gcacctggat ggaaaaggac gggctggccc aggaagaggt catggatgcc
     1921 ctgctggatc gccagggtcg cctctgggtg gcgaccgaaa gcgccggtat cacccggatg
     1981 gacggcgagg tgccggtcca gttcaacgaa cgcaacggac tgcccagcga cgtggtctac
     2041 ggcctgctgc tggaccgcga tggcagcgtg tgggcggcca ctgcgaacgg ggtggcccat
     2101 ttcgccaagg gccgcatccg caactacacg caacaggccg gcttgcccgc ggcgccgatc
     2161 ttccgggtca tgcaggacgc gagcggcacg ctgttcgtgg gcaccgagcg cggcgtctac
     2221 cgcatgcagg gcgaacgctt cgtcctgcaa tcgccgctgc tgcccgagga cggcgtgccc
     2281 agcctggcgc gggataccgc cggcaacctg tgggtcggca cggtcaacaa cggactggtg
     2341 cggctcggcg aatccggcgc ggagcgcttc accagcgtgc gcggcctgcc caacaatcgc
     2401 gtcgccgcgc tcctggtgga tcgcgagggc agcatctggg cgggcaccaa tgccggcctg
     2461 ctgcgcttga gcgatgcctc gttcagcacc tggaacggcg accaggggct cagcgacgac
     2521 tacgtccgtg cgttgtacga agcgccgcgt ggcggcatgt ggatcggcac cagccgcggc
     2581 ctgaacctgt ggcgtgacgg ccgcatcgag gccacctaca gcgccgcaga cggcctgccc
     2641 agcgattccg tgctgagcct gctggaatcc cgcgatggtt cgctgctggt cggcagctac
     2701 accgacggcg tgctgcgcat gcgcgacggc aaggtcgtgg gccactacga caatgcccgt
     2761 ggcatgcccg gcagcaacca ggtgcgcgcc ttggccgagg aagccgacgg caccctgtgg
     2821 atcggcacct cgcgtggcct ggcgcgttat cgcgatggca cgttcaaggc ctacggcctg
     2881 gccgagggac tcccccgcga attcatcatc tcgctgcaca tcgcccgcga cggcagcctg
     2941 tgggtcggga ccgcgaacgg ggccgccaac atcgtcgccg gcaaggtacg cagcctggac
     3001 atgcgcccgg tcaatggcgc gcaggacgtg ttcgcattcc acgaggacgc cgacggcacc
     3061 ttgtggctgg ccaccgatcg cggcctgctg cgctaccgga atggcgtgct gcgcgggctt
     3121 ggcctgaagg aaggcctgcc ggtcgacacg atgttcagcg tggtcgatga cgagcgtggc
     3181 cacctgtgga tgacctccaa ccgcggcgtg ctgcggatca agcgcagcga tgccgaagcg
     3241 gtgctcgagg ggcgcctggc gcaactgccc tacgatcatt tcggcgaggc cgacggcctg
     3301 gtcagcgcgc aatgcaacgg tggctccggg cccgcggcac tgcgcgacag ccgcggcaac
     3361 atctgggtgg cgacgtcgcg cggcgcggcg gtcaccaatc cggcccgcct gcaggcatac
     3421 cgccatcaat tgccgcaggt cgtcatcgaa caggcgtacg ccgacgacaa gccggtctcc
     3481 gccgaggacg ggctgctgcg cctgcctccg ggcacgcgga agctcgaatt ccgctacgcc
     3541 gccctcagct tcctgatgcc gcgcttcctg cgctaccgct atcggctgga aggcgaggac
     3601 gccgcgtgga tcgaacgcgg caacctgcgc gtggcccagt acaccaacct gcggccgggg
     3661 cgctatcggt tcctggtcga cgtctcggca ccgaccgtgg gccgcgactg gagcggcgaa
     3721 cccaccgtca tcgacatcga gatcaagccg caaccatggc agcaggcctg gttcctggcc
     3781 gtggcgacct tgctgctcgg cctgctgctg ttcgccatct accgttggcg gctgggcaac
     3841 ctgcgcaagc gcgccaccga actggaagac gtcatcgacc agcgcaccag cgcgctgcgc
     3901 gagcagaccg accggctgat ggagtcggac cgcgagaagt cgatcctgct ggcgcggctg
     3961 aaggaacagt cggaggcgtt cgagcggatg gcgctggaag atgcattgac cggcatcggc
     4021 aaccggcgcc acttcgatgc gcgcctgtcg cacgccttcg atcgcgcggt cgaaacgcgg
     4081 cagccgttga gtttcgcgtt gttcgacgtc gatgagttca agcggatcaa cgaccagtac
     4141 tcgcatgccg ccggcgacca cgccctggtg gcggtggcgc atgcgttgca ggcaggcatc
     4201 gagggccgcg gcagcatcgc gcgctggggc ggcgaggagt tcgccgtgct gttcgagggc
     4261 atgccgctgg acgaagcgcg ccagctctgc gagcagttgc gcgcgcgcgt gcaggccatc
     4321 gattgcacgg agttcgcgcc cggctggtcg atgacgatca gtgccggcgt caccgaacgc
     4381 acgggcctgg cgcaccacga gaaactggtc agtcgcgcgg atgcgctgct gtacgaggcg
     4441 aagcgggcgg ggcgcaaccg catctgcggt tgatctcgcc acctcccctt cgctgcgcga
     4501 aagggaggtg aatcgcaagg atcagccctt ggccttggcc agttgcaacc aggtatcgac
     4561 cacggtgtcg gggttcagcg agacggactc gatgccctgt tccatcagcc acagcgccag
     4621 gtccgggtga tcgctcggtc cctggccgca gatgccgatg tacttgccct tggccttcgc
     4681 cgcggagatc gccatcgaca gcatcttctt gaccgccggg tcgcgctcgt cgaacaggcc
     4741 ggcgacgatg gccgagtcgc gatccaggcc cagtgtcagc tgggtcatgt cgttggagcc
     4801 gatgctgaag ccgtcgaaga gctcgaggaa ttcgtcggcc atgagcgcgt tggacggcac
     4861 ctcgcacatc atgatgacct tcaatccatt ctcgccctgg acgaggccgt tctcgcgcag
     4921 cacatcgatt accttgcggc cttcgccgag cgtgcgcacg aacgggatca tcacccacag
     4981 gttgtccagg cccatgtcct cgcgcacgcg cttgaccgcc cggcattcca gcgcgaaggc
     5041 atccttgaag ctcgggtcga tgtaacgcga ggcgccacgg aacccgatca tcgggttctc
     5101 ctcgtgcggc tcgtacttgc tgccgccgat caggttggcg tactcgttcg acttgaagtc
     5161 ggacaggcgc acgatcaccg ggttcggcgc caccgatgcg gtgatggtgg cgatgccttc
     5221 ggccaggcgg tccacgtaga actccacggg ccccgcataa ccggcggtgc gcgcgtcgat
     5281 cttcgccttg gtcgcagcat cctggcggtc gtattcgagg agcgccttcg gatgcacgcc
     5341 gatgtggctg gcgatgatca tctccaggcg ggccaggccg atgcccgcgt tcggcagcat
     5401 gccgaaatcg aaggcgcggt ccgggttcgc cacgttcatc atgatcttca gcggcgccgg
     5461 cggcatgttg ccgaggtccg tcgtggtgcg ttcgaagggc agggcgcctg catagatgaa
     5521 gccggtatcg ccctcggcgc agctgaccgt gatcggatcg ccgtccttga cgctgtcgag
     5581 cgcgttaccg gtgccgacca ccgcgggcac gcccagctcg cgggcgatga tcgcggcgtg
     5641 gcaggtgcgg ccaccgcggt tggtgacgat ggcggcgctg cgcttcatca ccggctccca
     5701 gtcgggatcg gtcatgtcgg cgaccagcac gtcgccgggc tgcacgcgct ccatgtcggc
     5761 cagcgaacgc accacgcgcg cggtgccgct gccgatcttg ctgccgatcg cgcggccttc
     5821 cgccagcacg tcgccgcgct cgcccagcgt gtagcgctcg atctgggtcg catgcgcgcg
     5881 cgacttcacc gtctccggac gcgcctgcag gatgtagagc ttgccggtat ggccgtcctt
     5941 gccccactcg atgtccatcg gccggccgta gtgacgttcg atcaccagcg actgcttcgc
     6001 cagctcctgc acgtccgcat cggaaataga aaacgtgctg cgcaacgccg ccggcgtttc
     6061 ctcggtgcgc acgcgttcgc ccggcgcgtc ggaatacacc atgcgcaact gcttgctgcc
     6121 cagcgaacgg cgcaggatcg ccggcttgcc ggcattgagc gtgggtttgt agacgtagaa
     6181 ctcgtcggga ttcaccgcac cctgcacgac catctcgccc aggccgtagc tggcggtgac
     6241 gaagaccacg tcgcggaaac cggattcggt gtcgagggtg aacagcacgc cggatgaccc
     6301 cacgtcggag cgcaccatca gctgcacgcc ggcggacagg aacacgtcct cgtgggcgaa
     6361 gccgtggtgc acgcgatagg cgatggcgcg gtcgttgtac aggctggcga acacttcctt
     6421 gaccttgtgc acgacgtcgt cggcgccggt cacgttgagg aaggtttcct gttggccggc
     6481 gaagcttgca tcgggcaagt cttccgcggt cgccgaggaa cgcaccgcca ccgcgatgtc
     6541 gttcgatccg gcatccgcgc acagcttcgc gtacgcactg cggatgtcgg catccagttc
     6601 cggctgcagc ggcgcgtcga tcacccagcc gcggatttcg gcgccagcgc gggtcagcgc
     6661 gggcacgtcc tcgacatcga ccgtggcgag cttgtcgtag atgcgcgcat gcaggtcgtt
     6721 gtgggcgatg aaggccttga acgcgtcggc agtggtggcg aacccgcctg gcaccgagac
     6781 gccgagcccc gagagctcgc cgatcatctc gccgagcgag gaattcttgc cgcccacctg
     6841 ggcgaggtcg gtcaggcgca ggtcgttcag ccacaggacg ttgttgatcg aagcgggggc
     6901 gctcaagaca ggctccagtg caggggaggg cggcgcgacc gcgccgaagc ggctatttta
     6961 caaccctcgc cccgcacgct ggccacgaat gcacatggat cacccacgcc ccgtgttcta
     7021 cgtctccgat ggcaccggta tcaccgcgga aaccattggc cacagcatgt tgacccagtt
     7081 cagcgggatg cgcttccaga ccgaccggct gccgttcgtg gacacccccg agaaggcgcg
     7141 cctggtggcg gcgcggatcc gggcggcggg cgaagcgagc ggc
//
LOCUS       DAOOCX010000109         7106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_9890_length_7106_cov_8.064955, whole genome shotgun sequence.
ACCESSION   DAOOCX010000109 DAOOCX010000000
VERSION     DAOOCX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9890_length_7106_cov_8.064955"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PK399_09630"
     CDS             <1..298
                     /locus_tag="PK399_09630"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812978.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPW13419.1"
                     /translation="AIPKALKQAGLTKDQLDWIELNEAFAAQALAVIRDSELDASKIN
                     PLGGAIALGHPLGATGAIRTATIVHGMRRHQKKYGMVTMCIGTGMGAAGIFESL"
     gene            complement(367..1275)
                     /gene="galU"
                     /locus_tag="PK399_09635"
     CDS             complement(367..1275)
                     /gene="galU"
                     /locus_tag="PK399_09635"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HPW13420.1"
                     /translation="MSVPANPRIRVAVFPVAGLGTRFLPATKTVPKEMLPIIDKPLIQ
                     YAVDEAIEAGCDTLVFVTNRYKHAVADYFDKAYELEQKLERSGKLEQLELVRNVLPSH
                     VRAVFVTQAEALGLGHAVLCARPIIGDQPFAVLLPDDLIWNRGPGALKQMADLAEASG
                     ASVIAVQDVPRSQTGSYGIVATDSFEGRSGRINAIVEKPAPEASPSTLAVVGRYVLDG
                     RIFDLLEQTAPGAGGEIQLTDAIASLLAEKPVQAYRFQGTRFDCGTHIGLIEATIRYA
                     LDHETLSDAARDLMKNALDEMGVVET"
     gene            complement(1281..3191)
                     /locus_tag="PK399_09640"
     CDS             complement(1281..3191)
                     /locus_tag="PK399_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPW13421.1"
                     /translation="MSTWKDRLKYRLPRLAVVLHDLVMVWVCWQGLHYMRYALQPSPL
                     SLAPFSSTVLIVLVAQGLVSWRVGLYRGLWRFASVPDLMNIFKASLLGLLAVVVGLFF
                     YSRLDLVSRAALLLYPFALTILLGAPRLIFRAWKDHRLLQTADGAERVLILGAGQAGE
                     ALVRDLRRTGRYDPIGFLDDSVGLRGTRLQGLPVLGKVDDLRRIARETGAQLLVIAMP
                     SVSATAMRRVIGLCEQSGLPFRTVPRLADILEGRSLPGQLKEVAIEDLLGRQPHTPDW
                     KAIRGWLGARSVLVTGAGGSIGLELCRQCARHGAHRITLVDIDELALLTAETELKRDF
                     QGIECILVLGDCGDPAVIAHALRKAEPEAVFHAAAYKQVPLLQGQLREAVRNNVLATD
                     VVARACRDAKVATFVLISTDKAVDPVNVLGATKRLAEMVCQGLVDPRSTRMVTVRFGN
                     VLDSAGSVVPLFREQIRRGGPVTVTDRDVTRYFMTIPEACQLILQASSIGAQQAVYTL
                     DMGEPVSISMLAEQMIRLAGKQPERDIAIEYTGLRPGEKLHETLFHADERYSATTYPN
                     ILQAAARSVVPERVEHALEVLRAAVRDYDLERLADALRDAVPEFNPSDDTEVAHVATV
                     VAFPARRARQPQ"
     gene            complement(3188..4186)
                     /locus_tag="PK399_09645"
     CDS             complement(3188..4186)
                     /locus_tag="PK399_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW13422.1"
                     /translation="MRWSEPVVLAACAVAALVVSMACTAWAIAYARRCGMLDQPGERR
                     SHATATPRGGGVGIAVALLLAMCLLGGWVDAGWWPIAGGLLLVAGVGWGDDHRPLPIA
                     LRLGAHALAGVALAWGLHLQDAGLPAVLAALVLVPVLVNAWNFMDGIDGLATSQALLC
                     AAALASLLGGGWQVLGVALAGACLGFLPFNLPTARIFLGDVGSGALGYGMAAMLGGAI
                     ATLPAATWPLVLLPPMAMLGDSGLTLCWRVATGQRWWEPHVQHLFQRSSRRWGHRWVT
                     TAYAVWTCVAIGIMLRAHGMRMPGTLLVAAVMAAAWLAAWTWMHRRSDTTTEGFGT"
     gene            complement(4173..5366)
                     /gene="lapB"
                     /locus_tag="PK399_09650"
     CDS             complement(4173..5366)
                     /gene="lapB"
                     /locus_tag="PK399_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HPW13423.1"
                     /translation="MEFLDQWVWFVVLLPLAALAGWVIGRRGGERHSDSQVSKLSTTY
                     FRGLNYLLNEQPDKAIELFLHIAELDKDTFETQVALGHLFRRRGEVDRAIRLHQALAQ
                     RTDLSDAQKVQALSALGEDYMRSGLLDRAETVFSDLAQLDQRAPQALRHLIGIYQAER
                     DWEKAIENAQRFEAATGESMGKLVAQFECELADRLRSAGDSEAARGAIKRAYAADSTS
                     VRAGITEGRIEAEAGNPAGAVRAFERAARHDPDYLPVILPALLESYAKVGERTGARAF
                     LAEMSEHYRGISPVLALTRMIEAEEGVGAARRYLGQQLKDRPSVRGEAALIDLTIAEH
                     PEMEQADARATLHDLKHITDQLLVRNPSYRCTRCGFGARSHHWQCPSCKEWGTVKPLL
                     NYAVV"
     gene            complement(5373..5630)
                     /locus_tag="PK399_09655"
     CDS             complement(5373..5630)
                     /locus_tag="PK399_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HPW13424.1"
                     /translation="MRLLRLIAAFACIALGAIVGALNTQHVVLDLGFTTIPTSLGLGM
                     LLALLLGVLVGGTIIAVGVVAPMRRRLRRAEATARLPRHQD"
     gene            complement(5675..5977)
                     /locus_tag="PK399_09660"
     CDS             complement(5675..5977)
                     /locus_tag="PK399_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HPW13425.1"
                     /translation="MTKSELIELLARRQPHLKADDVDMSVKSLLQMMGSALAVGERIE
                     VRGFGSFSLHYRPPRTGRNPKTGDAVALPGKHVPHFKPGKELRERVSPVVPLPTDD"
     gene            complement(6054..>7106)
                     /locus_tag="PK399_09665"
     CDS             complement(6054..>7106)
                     /locus_tag="PK399_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPW13426.1"
                     /translation="GGIDGLLHITDMAWKRVRHPSEVVEVGAELDVRVLKYDKERNRV
                     SLGLKQLGEDPWDNIARRYPSNSRVFGKVSNVTDYGAFVEIEPGVEGLVHVSEMDWTN
                     KNVNPAKVVQVGDEVEVMVLDVDEERRRISLGIKQVTSNPWETFAAIHKKNDKVSGQI
                     KSITDFGIFIGLDGGIDGLIHLSDISWNSTGEDIARNYKKGDTLEAVVLAVDPERERI
                     SLGVKQMEQDPLGQFMAANAKGTKVTGTVKEVDAKGATIDLGDGVEGYVMARDVADER
                     VEDASQYLKVGQEIEAKFVGMDRKGRSLQLSIKGKDDAETAEAMAEYNKAASDASSGT
                     TKLGALLREQLNSKSE"
BASE COUNT         1129 a         2446 c         2391 g         1140 t
ORIGIN      
        1 ggcgatcccg aaggcgctga agcaggcggg cctgaccaag gatcagctgg actggatcga
       61 actgaatgaa gccttcgccg cacaggcgct ggcggtcatc cgcgacagcg aactggatgc
      121 atcgaagatc aacccgctgg gtggtgccat cgccttgggc catccgctgg gtgcgaccgg
      181 cgcgatccgc accgcgacca tcgtccatgg catgcgccgc caccagaaga agtacggcat
      241 ggtgacgatg tgcatcggca ccggcatggg cgcggcggga atcttcgagt cactctgagc
      301 ttgctgtcgt tggatgcgaa aacggcgcct tgaggcgccg ttttcgttgg tgacccgtag
      361 ccgcgatcag gtctcgacga cgcccatttc gtccagcgca ttcttcatca ggtcgcgcgc
      421 ggcatcgctc agcgtctcgt ggtccagcgc gtagcggatg gtcgcctcga tcaggccgat
      481 gtgggtgccg cagtcgaagc gcgtgccctg gaagcgatag gcctgcacgg gcttttccgc
      541 cagcaaggaa gcgatggcgt cggtgagctg gatctcaccg ccggcacccg gcgcggtctg
      601 ttccagcagg tcgaagatcc gtccatccag cacgtagcgg ccgaccacgg ccagggtgct
      661 tggcgatgcc tcgggcgccg gcttttcgac gatcgcgttg atccgccccg accgcccttc
      721 gaaggagtcg gtggcgacga tcccgtaact gccggtctgc gaacgaggca cgtcctgcac
      781 cgcgatcacg ctggcgccgg acgcttccgc gaggtcggcc atctgcttga gcgcgccggg
      841 accgcggttc cagatcaggt catcgggcaa cagcacggcg aacggctggt cgccgatgat
      901 gggcctggcg cacagcacgg catgccccag ccccagcgcc tcggcctggg tcacgaagac
      961 cgcgcggaca tgcgatggca gcacgttgcg gaccagctcc agctgttcga gcttgccgct
     1021 gcgctccagc ttctgttcga gctcgtacgc cttgtcgaaa tagtccgcga ccgcatgctt
     1081 gtagcggttg gtgacgaaga ccagggtgtc gcagcccgcc tcgatcgcct cgtccaccgc
     1141 gtactggatc agcggcttgt cgatgatcgg cagcatttcc ttcggcacgg tcttggtggc
     1201 cggcaggaac cgggttccca ggccagcgac cggaaacacg gcgacgcgga tgcgcgggtt
     1261 ggcgggtacg gacattgacg tcactgcggc tggcgggcgc gacgcgcggg gaacgcgacc
     1321 accgtggcga catgggccac ctcggtatcg tcgctcgggt tgaactcggg gacggcatcg
     1381 cgcagggcat ccgccaggcg ctccaggtcg tagtcgcgca ccgccgcgcg cagcacctcg
     1441 agcgcgtgct cgacgcgctc cggtaccacg ctgcgggccg ccgcctgcag gatgttcgga
     1501 taggtcgtgg cgctgtagcg ctcgtccgcg tggaacagcg tttcgtgcag cttctcgccc
     1561 gggcgcaaac cggtgtactc gatcgcgatg tcgcgctccg gctgcttgcc cgccagccgg
     1621 atcatctgct cggccagcat cgagatcgac accggttcgc ccatgtcgag cgtatagacc
     1681 gcctgctgcg caccgatcga ggacgcctgc aggatcagct ggcaggcctc gggtatggtc
     1741 atgaagtagc gggtcacgtc gcggtcggtg acggtgaccg ggccgccgcg gcggatctgc
     1801 tcgcggaaca gcggcaccac gctgccggcc gaatccagca cgttgccgaa gcgcacggtg
     1861 accatgcgcg tcgaacgcgg gtccacgagt ccttggcaga ccatttcggc caggcgcttg
     1921 gtcgcgccga ggacgttgac cggatccacc gccttgtcgg tggagatcag cacgaaggta
     1981 gcgaccttgg cgtcgcggca ggcacgcgcg accacgtcgg tggccagcac gttgttgcgc
     2041 acggcctcgc gcagctgtcc ctgcagcagg ggcacttgct tgtaagcggc ggcgtggaag
     2101 accgcttccg gttcggcctt gcgcaaggca tgcgcgatca ccgccggatc cccgcaatca
     2161 ccgaggacca ggatgcactc gatgccctgg aaatcgcgct tgagttcggt ttccgccgtg
     2221 agcaacgcga gttcgtcgat gtcgaccagg gtgatccggt gcgcgccatg gcgcgcgcat
     2281 tgccgacaca attccagccc gatcgagccg ccagcgcccg tcaccagcac cgaacgcgcg
     2341 cccagccagc cgcggatcgc cttccagtcc ggggtgtgcg gttgccggcc cagcaggtcc
     2401 tcgatcgcca cttccttgag ctggcccggc aacgagcggc cctcgaggat gtcggccaac
     2461 cgcggcacgg tgcggaacgg cagcccgctc tgctcgcaca ggccgatgac ccgccgcatc
     2521 gccgtggcgg agaccgacgg catcgcgatc accagcagct gcgcgccggt ctctcgggcg
     2581 atgcggcgca ggtcatcgac cttgccgagc accggcaggc cctgcaggcg cgtgccgcgc
     2641 aggccgaccg aatcgtccag gaagccgatg gggtcgtagc gcccggtgcg gcgcaggtcg
     2701 cgcaccagtg cttcgccggc ttggccggcc cccaggatca ggacgcgctc ggccccgtcg
     2761 gcggtctgca gcaggcggtg gtccttccat gcacggaaga tcagccgtgg cgcccccagc
     2821 aggatggtca acgcgaacgg atacagcagc agcgccgccc gcgacacgag gtccagccgg
     2881 ctgtagaaga acaagccgac gaccaccgcc agcaggccaa gcaggctggc cttgaagatg
     2941 ttcatcaggt ccgggacgct ggcgaaccgc cacagcccgc ggtacaggcc gacgcgccag
     3001 gagaccaggc cctgcgcgac cagcacgatc agcaccgtgc tggagaaagg cgccagtgac
     3061 agggggcttg gctgcagcgc gtagcgcatg tagtgcagcc cctgccagca gacccagacc
     3121 atgaccaggt catgcagcac gaccgccagg cgtggcaggc ggtacttgag gcgatccttc
     3181 catgtgctca tgtgccgaat ccttcggtcg tggtgtcgct gcgccggtgc atccaggtcc
     3241 aagccgccag ccaggccgcc gccatcacgg cggccaccag cagcgtgccg ggcatccgca
     3301 tgccatgcgc gcgaagcatg atcccgattg ccacgcacgt ccataccgca tacgcggtgg
     3361 tcacccaccg atgtccccac cgccggctgc tgcgttggaa caggtgctgg acgtgcggct
     3421 cccaccagcg ctggccggtg gccacgcgcc agcagagggt caggccgctg tcgcccagca
     3481 tggccatcgg cggcaacaag acgagtggcc acgtcgccgc gggtagcgtg gcgatggccc
     3541 cgcccagcat cgccgccatc ccgtagccaa gtgcgccact gcccacgtca cccaggaaga
     3601 tccgcgccgt cggcaggttg aacggcagga acccgaggca ggcgcccgcc agcgcgacgc
     3661 ccaggacctg ccagcctcca ccgagcaggc tggccagtgc ggcggcgcac agcagcgcct
     3721 ggcttgtggc caggccatcg atgccatcca tgaagttcca ggcattgacc agcaccggca
     3781 ccagcaccag cgcggccagc accgccggca ggcccgcgtc ctgcaggtgc agcccccacg
     3841 ccagtgccac gccggccagg gcgtgtgcgc cgaggcgaag cgcgatcggc agcgggcgat
     3901 gatcgtcgcc ccaccccacc ccggcaacca atagcaggcc gcccgcgatc ggccaccagc
     3961 cggcatccac ccatccaccg agcaggcaca tcgccagcaa cagcgcgacc gcgatgccga
     4021 cgccgccgcc gcgcggcgtc gccgtcgcat ggctgcggcg ctcgccaggc tggtcgagca
     4081 tcccgcaccg gcgcgcgtag gcgatcgccc acgcggtaca tgccatcgag accaccagcg
     4141 cagcgaccgc gcaggcggcc agcacgaccg gttcagacca ccgcatagtt cagcaatggc
     4201 ttcaccgtgc cccactcctt gcaactcggg cattgccagt gatggctgcg tgcgccgaag
     4261 ccgcaacggg tgcagcgata gctgggattg cgcaccagca actggtcggt gatgtgcttg
     4321 aggtcgtgga gggtcgcgcg tgcgtccgcc tgctccattt cggggtgttc cgcgatggtc
     4381 aggtcgatca atgccgcttc gccgcgcacc gacgggcgat ccttcagttg ctggcccagg
     4441 tagcgccgcg ccgcgccgac gccctcctcg gcctcgatca tccgggtcag cgccagcacc
     4501 ggcgagatgc cccgatagtg ttcgctcatc tcggccagga aggcgcgcgc gccggtgcgt
     4561 tcaccgacct ttgcgtagct ctccagcagc gccggcagga tcaccggcag gtagtccggg
     4621 tcgtggcggg ccgcgcgttc gaaggcgcgc accgcgccag cggggttgcc ggcctcggct
     4681 tcgatccggc cttcggtgat gccggcgcgc accgaggtcg aatcggcggc ataggcacgc
     4741 ttgatcgcgc cacgcgcggc ttcgctgtcg ccggcggatc gcagccgatc ggccagttcg
     4801 cattcgaact gcgccaccag cttgcccatc gactcgccgg tggcggcctc gaagcgctgt
     4861 gcgttctcga tcgccttttc ccaatcgcgc tcggcctggt agatgccgat caggtggcgc
     4921 aacgcctgcg gcgcgcgctg gtcgagctgg gcgaggtcgc tgaacacggt ttcagcgcgg
     4981 tccagcaggc ccgatcgcat gtagtcctcg cccagcgcgg acagcgcctg gaccttctgc
     5041 gcatcgctca ggtcggtgcg ctgcgccagc gcctggtgca ggcggatggc gcgatccacc
     5101 tcgccgcgcc ggcggaacag gtggcccagc gcgacctggg tctcgaaagt gtccttgtcg
     5161 agttccgcga tgtgcaggaa caactcgatc gccttgtcgg gctgctcgtt caacaggtag
     5221 ttgaggccgc ggaaataggt ggtcgacaac ttgctgacct ggctgtcgct gtggcgttcg
     5281 ccaccgcggc ggccgatcac ccagcccgcc agcgccgcca acggcagcag gacgacgaac
     5341 cagacccatt ggtcgaggaa ttccatggcg tgtcagtcct ggtggcgcgg caggcgtgcg
     5401 gtggcttcgg cccggcgcag gcggcggcgc atcggagcga ccacgcccac ggcgatgatg
     5461 gtgccaccga ccaggacacc cagcagcaag gccagcagca tgccaagccc gaggctggtg
     5521 gggatggtgg tgaaaccgag gtcgagcacg acgtgttggg tgttgagtgc gccgacgatg
     5581 gcgccgagcg cgatgcaggc gaacgcggcg atgaggcgga gcaggcgcat cggggactcc
     5641 gggatcgcct gccagcataa cggactgcgg cggttcagtc gtccgtgggc aaggggacga
     5701 ccgggctcac ccgttcgcgc agttccttgc cgggcttgaa atgcggcacg tgcttgcccg
     5761 gcaacgcgac ggcatcgccg gtcttcgggt tgcggccggt acgtggcggc cggtagtgca
     5821 gggaaaaact gccgaacccg cgcacctcga tgcgctcgcc cacggcgagc gcgctgccca
     5881 tcatctgcag cagcgacttg accgacatgt cgacatcgtc ggccttgagg tggggctggc
     5941 gtcgcgccag caattcgatg agttcggact tggtcatgga gccacgcggt gtgctgccaa
     6001 gaaaatggcg gcccggcctg ggccggaccg ccatgatcgt caggccgtca ggatcactcg
     6061 gacttgctgt tcagctgctc gcgcagcagc gcacccagct tggtggtgcc gctggaagcg
     6121 tcggaagcgg ccttgttgta ctcggccatc gcctcggcgg tctcggcatc gtccttgccc
     6181 ttgatcgaga gctgcaggct gcggcccttg cggtccatgc ccacgaactt ggcctcgatt
     6241 tcctggccga ccttcaggta ctggctggca tcctcgacgc gctcgtcggc gacgtcgcgg
     6301 gccatcacgt agccttccac gccatcgccc aggtcgatgg tcgcgccctt ggcgtccact
     6361 tccttgacgg tgccggtgac cttggtgccc ttggcattcg ccgccatgaa ctggcccagc
     6421 ggatcctgtt ccatctgctt gacgcccaag ctgatgcgct cgcgctccgg gtcgaccgcc
     6481 agcaccaccg cttcgagcgt atcgcccttc ttgtagttgc gggcgatgtc ttcgccggtg
     6541 gagttccagc tgatgtcgga caggtggatc aggccgtcga tgccgccgtc caggccgatg
     6601 aagatgccga agtcggtgat cgacttgatc tggccggaca ccttgtcatt cttcttgtgg
     6661 atcgcggcga aggtctccca cgggttgctg gtgacctgct tgatgcccag cgagatgcgg
     6721 cgacgctcct cgtccacgtc cagcaccatg acctcgactt cgtcgccgac ctgcacgacc
     6781 ttggccgggt tgacgttctt gttggtccaa tccatctcgg acacgtggac caagccttcc
     6841 acgcccggct cgatctcgac gaacgcgccg taatcggtga cgttgctgac cttgccgaac
     6901 acgcggctgt tggacggata gcggcgggcg atgttgtccc acggatcctc gcccagctgc
     6961 ttcaggccca gcgacacgcg gttgcgctcc ttgtcgtact tgagcacgcg cacgtcgagc
     7021 tcggcgccga cttcaacgac ttcggacgga tggcgcacgc gcttccacgc catgtcggtg
     7081 atgtgcagca ggccgtcgat gccgcc
//
LOCUS       DAOOCX010000110         7105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_9896_length_7105_cov_7.634326, whole genome shotgun sequence.
ACCESSION   DAOOCX010000110 DAOOCX010000000
VERSION     DAOOCX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9896_length_7105_cov_7.634326"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /gene="accC"
                     /locus_tag="PK399_09670"
     CDS             <1..1329
                     /gene="accC"
                     /locus_tag="PK399_09670"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017911501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPW13427.1"
                     /translation="ALRILRACHALGIRTVAVHSTVDRNLKHVAMADESVCIGPAPSS
                     ESYLNMASIIAAAEVTDAQAIHPGYGFLSENADFAERVEQSGFVFIGPKADTIRLMGD
                     KVEAIRAMKAAGVPCVPGSGGPLGDDAATNIRIAAEIGYPVIVKAAGGGGGRGMRVVQ
                     TEAALANAISTTKQEAKAAFGNDMVYMEKFLENPRHVEIQVLADGQGNAIHLGERDCS
                     MQRRHQKVVEEAPAPGITEQARAEIGKVCVDACLRIGYRGAGTFEFLYEDGRFYFIEM
                     NTRIQVEHPVTELVTGIDLVREQLLIASGHKLSIKQSDVVLKGHAIECRINAEDPDTF
                     MPSPGLVSHFHSPGGPGVRVDSHIYEGYRVPPNYDSMIGKLIVHGADRAQAIARMRVA
                     LSEMVVDGIKTNVPLQQRILADGGFAQGGQNIHYLEKRLAERKEKALSIL"
     gene            1404..2726
                     /locus_tag="PK399_09675"
     CDS             1404..2726
                     /locus_tag="PK399_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13428.1"
                     /translation="MQRRHALYLAFAATLVALPASSQQGKNPPTNLYIDVLTHNMAGM
                     PDMGGMMGGLGGFMARRMGGADTGKPTYPTTRAGGMTGQYLDIALHNSLKPGIEAADQ
                     IPGGLKLGKSLTLIPIDPSKPTEGSTPPGKIPDVTIKITEYWGCGASVRKGQPKVASF
                     TIKGGNKSIDPNNPMGSMQGVDFQQSGSLSKTIPVQDRDIDLKPGWVYWPNRKHGKQV
                     PNGARLAGDHRITGDGIPASMQFQVQENADFMPKLALRTQGEATDAIGLSWPMVERAR
                     GYHITGMHMQVLGENSYAMTLWSSAEVPGAGQDLHTNLSAGQLDKWLKQKVLLASTAT
                     SCTIPKGIFSGASNVEGQQATMPGMLSMTAYGPESWITYPPKPADPKQPWNPEWSVRL
                     RARSSATAILGLDFGGMQQMDDGDGEDQQQQPQKKPGMKGLLKGILGG"
     gene            2794..3522
                     /locus_tag="PK399_09680"
     CDS             2794..3522
                     /locus_tag="PK399_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13429.1"
                     /translation="MPQTTARDAVQEGPPASARSGAKRALDAAVVNIELTLISIIQGL
                     ALGVLAAASVEPLVQLQWQAWPYVLTGLLVVLIFWSRALVHTLSFIGWPLEFGHTFVY
                     FGATLLEAVALSQVAHPERWFALNTLYAVAVWGLYAYDTRVVRAHAADFGTADEQALL
                     ADILRDQRVNTRFMMPAAVAFQGLAWWLVHAYPHAFIDRGWHLLLIGLTLLFSLNYLV
                     GGVRILHRRQAWIVARHAQELHEA"
     gene            3571..4500
                     /gene="prmA"
                     /locus_tag="PK399_09685"
     CDS             3571..4500
                     /gene="prmA"
                     /locus_tag="PK399_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPW13430.1"
                     /translation="MPYLQLTLPCSETDQPRYERTLEDVGALAVSLQDAHLDDADEQA
                     IFEPGVGEIPLWDEMTITALFPAETDGLVLLAAMESFDPGLDWSKVRFETVEDQDWER
                     AWMDQFQPMQFGARTWIVPWNHDLPGEADTPGAAVIRLDPGLAFGSGTHPTTALCLRW
                     LDGLATEGALQGRDVLDFGCGSGILALAAMKLGAARAIGVDNDPQAIIATADNAERNG
                     VRIDTFMPADEPAATYPVVVANILASALDALAETLAARTAPAGRIAMSGILAGQEDDL
                     LLRFAPWFDALRVAREDDWMRIEGVRNARPVSA"
     gene            4497..5006
                     /locus_tag="PK399_09690"
     CDS             4497..5006
                     /locus_tag="PK399_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HPW13431.1"
                     /translation="MSRTPSFALRDAPAATRRRGEWLALAVLVLAFAGQLFFGERAVL
                     AANAQWRPLVVAACDVARCSLPPWREPTAFTMLARDVIAVPQHPGVLRVQASFRNDAR
                     WAQAWPALVLTLSDADNRTLGARRFLPRDYLGDAPSSTLLAPGQASQLAFDIVEPAPN
                     VVAFDFRFD"
     gene            5168..5431
                     /gene="fis"
                     /locus_tag="PK399_09695"
     CDS             5168..5431
                     /gene="fis"
                     /locus_tag="PK399_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011257462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HPW13432.1"
                     /translation="MNVSADRQDASRAPLRDHVATSVRRYLRDLDGCDTDDLYNIALR
                     ELEIPLFAEVLRHCDGNQSRAASMLGIHRATLRKKLKDYGLSS"
     gene            5537..>7105
                     /gene="purH"
                     /locus_tag="PK399_09700"
     CDS             5537..>7105
                     /gene="purH"
                     /locus_tag="PK399_09700"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPW13433.1"
                     /translation="MTTNLSEGPSVAIRRALLSVSDKTGLLDLAKALASRGVELLSTG
                     GTAKALRDAGLAVKDVSEHTGFPEMMDGRVKTLHPKVHGGLLGRAGIDDAVMAEHGID
                     AIDLLVLNLYPFAKVSMDPSSTFEDVIENIDIGGPAMLRSAAKNFARVAVATDPAQYA
                     GIVDELNASAGTLSAKTRFALAVAAFNNVAFYDACISNFLSAKQEDGSHAQFSAQANG
                     NFVKVMDLRYGENPHQQAAFYRDLWPAPGSLATFTQLQGKELSYNNIADSDAAWECVR
                     QFDAPACVIVKHANPCGVAVGAACGDAYELAYATDPTSAFGGILAFNTKLDAATCKAI
                     LDRQFVEVLIAPDYEDGALEYARKKANVRVLRIPMAKPSPGFIDTKRVGSGMLMQTAD
                     DRVITRDDLKVVTKLAPTEAQFADLLFAWKVAKFVKSNAIVYAKENRTIGVGAGQMSR
                     VYSARIAGIKAHDANLQVEGSVMASDAFFPFRDGIDAAAEAGIKAVIQPGGSMRDAEV
                     IAAADEHGLAMVFTG"
BASE COUNT         1205 a         2507 c         2332 g         1061 t
ORIGIN      
        1 gcgctgcgca tcctgcgcgc ctgccacgcg ctgggcatcc gcacggtcgc cgtgcattcc
       61 accgtcgacc gcaacctcaa gcacgtggcg atggccgacg aatccgtctg catcggcccg
      121 gcgccgtcca gcgagagcta cctcaacatg gcctcgatca tcgccgccgc cgaggtgacc
      181 gatgcgcagg cgatccaccc cggttacggc ttcctctccg agaacgccga cttcgccgag
      241 cgggtcgagc aatccggctt cgtcttcatc gggccgaagg ccgacacgat ccgcctgatg
      301 ggcgacaagg tcgaggcgat ccgcgcgatg aaggccgccg gcgtgccctg cgtgcccggc
      361 agcggcggcc cgctcggcga cgacgccgcc accaacatcc gcatcgccgc ggagatcggc
      421 tacccggtga tcgtgaaggc cgccggcggt ggtggcggac gcggcatgcg cgtggtgcag
      481 accgaggccg cgctggccaa cgccatcagc acgaccaagc aggaagccaa ggccgcgttc
      541 ggcaacgaca tggtctacat ggagaaattc ctggagaacc cgcgccacgt ggagatccag
      601 gtgctggccg acggccaggg caacgcgatc cacctgggcg agcgcgactg ctcgatgcag
      661 cgccgccacc agaaggtggt cgaggaagcg cccgcgcccg gcatcaccga gcaggcacgc
      721 gcggagatcg gcaaggtctg cgtggacgcc tgcctgcgca tcggctatcg cggcgccggc
      781 accttcgaat tcctgtacga agacggccgc ttctacttca tcgaaatgaa cacccgcatc
      841 caggtcgagc atccggtcac cgaactggtc accggcatcg acctggtccg cgaacagttg
      901 ctgatcgcct ctggccacaa gctctcgatc aagcagtcgg atgtggtgct gaagggccac
      961 gcgatcgagt gccgcatcaa cgcggaagac ccggacacct tcatgccttc gccgggcctg
     1021 gtcagccact tccattcgcc gggcggcccg ggcgtgcgcg tcgattcgca catctacgaa
     1081 ggctaccggg tgccgccgaa ctacgactcg atgatcggca agctcatcgt ccacggcgcc
     1141 gaccgcgcgc aggcgatcgc gcgcatgcgc gtggcgttga gcgaaatggt ggtggacggc
     1201 atcaagacca atgtgccgct gcagcaacgc atccttgcgg atggcggttt cgcgcagggc
     1261 gggcagaaca tccattacct ggaaaagcgc ctggccgagc gcaaggaaaa agcgctgtcg
     1321 atcctctgag ccgaggtggc gtgaccgctt cggggccgat tttccgcttt actgtgggta
     1381 tcgacccgtc gtccggagcc gccatgcaac gccgccacgc cctgtacctc gccttcgccg
     1441 ccaccctcgt cgccctgccc gcctccagcc agcagggcaa gaacccgccg accaacctct
     1501 acatcgacgt gctcacccac aacatggccg ggatgccgga catgggcggg atgatggggg
     1561 gcctcggcgg cttcatggcc cgccgcatgg gcggcgccga caccggcaag ccgacgtatc
     1621 cgaccacgcg cgctggcggc atgaccgggc agtacctgga catcgccctg cacaacagcc
     1681 tcaagccggg catcgaggcg gccgaccaga tcccgggcgg actcaagctc ggaaagtcgc
     1741 tgaccctgat cccgatcgac ccgagcaaac cgaccgaagg cagcacgccg cccggcaaga
     1801 tcccggacgt gaccatcaag atcaccgagt actggggctg cggtgccagc gtgcgcaagg
     1861 gccagcccaa ggtcgcctcc ttcacgatca agggcggcaa caagagcatc gaccccaaca
     1921 acccgatggg tagcatgcag ggggtcgatt tccagcagag cggcagcctc tcgaagacca
     1981 tcccggtgca ggatcgcgac atcgacctga aaccgggctg ggtgtactgg ccgaatcgca
     2041 agcacggcaa gcaggtgccg aacggcgcgc gcctcgccgg cgaccaccgg atcaccggtg
     2101 acggcatccc cgcctcgatg cagttccagg tccaggaaaa cgcggatttc atgcccaagc
     2161 tcgcgttgcg cacgcagggc gaggcgaccg acgcgattgg cctgagctgg cccatggtcg
     2221 agcgcgcccg cggttaccac atcaccggca tgcacatgca ggtgctgggg gagaattcct
     2281 acgcgatgac gctctggagc agcgccgaag tccccggtgc cggccaggac ctgcatacca
     2341 acctcagcgc cggccagctg gacaaatggc tgaagcagaa ggtcctgctg gcctcgaccg
     2401 cgaccagttg caccattccc aagggcatct tctcgggtgc ctccaacgtg gaaggccagc
     2461 aggcgaccat gcccggcatg ctcagcatga ccgcctatgg ccccgagagc tggatcacct
     2521 acccgcccaa gccggcggat cccaagcagc cgtggaaccc ggaatggagc gtccgcctgc
     2581 gcgcacgttc cagcgccacc gcgatcctcg gcctggattt cggcggcatg cagcaaatgg
     2641 atgacgggga cggcgaggac cagcaacagc agccacagaa gaagccgggc atgaagggcc
     2701 tgctgaaggg catcctgggc ggctgatcgc cagcgggctt gtcgccgcgt gcctgcgcgg
     2761 cgaccatggc ggcaggccac cgggcaagcc gccatgccgc agacgacagc acgcgacgcc
     2821 gtgcaggagg ggccaccggc ctccgcgcgt tccggcgcga agcgggcgct ggatgcggcc
     2881 gtcgtcaaca tcgagctgac cctcatcagc atcatccagg gcctggccct gggcgtgctg
     2941 gccgccgcca gcgtcgagcc gctggtgcaa ctgcaatggc aggcgtggcc gtacgtgctg
     3001 accggcctgc tggtggtgct gatcttctgg tcgcgcgcgc tggtgcacac gctgtccttc
     3061 atcggctggc cgctcgagtt cggccacacc ttcgtctatt tcggcgccac gttgctggag
     3121 gcggtggcgc tcagccaggt cgcccatccc gagcgctggt ttgcgctcaa tacgctgtat
     3181 gcggtggcgg tgtgggggct ttacgcctac gacacgcggg tggtgcgcgc gcacgccgcc
     3241 gacttcggca ccgcggacga acaggccctg ctggccgaca tcctgcgcga ccaacgggtc
     3301 aacacccgct tcatgatgcc ggccgcagtc gcgttccagg gcctggcctg gtggctcgtg
     3361 catgcctatc cccacgcctt catcgaccgc ggctggcacc tgctgctgat cgggctgacc
     3421 ctgctcttca gcctcaacta cctggtcggc ggggtgcgca tcctgcaccg ccggcaggca
     3481 tggatcgtgg cccgccacgc gcaggaattg cacgaggcct gagcacgccg gccattcccg
     3541 ccgcggccgc gatggccaat acttgccgcc atgccctacc tgcaactgac cctgccctgc
     3601 agcgaaaccg accagccccg ctacgagcgc acgctcgagg acgtcggcgc gctcgcggtg
     3661 agcctgcagg atgcgcacct ggacgatgcc gacgagcagg cgatcttcga acctggcgtg
     3721 ggcgagatcc cgctgtggga cgagatgacg atcaccgcac tgttcccggc cgagaccgac
     3781 ggactcgtcc tgctggcggc gatggagtcc ttcgatcccg gcctggactg gtcgaaggtg
     3841 cgcttcgaga cggtcgaaga ccaggactgg gaacgcgcgt ggatggacca gttccagccg
     3901 atgcagttcg gcgcgcgcac ctggatcgtg ccctggaacc atgaccttcc cggcgaggcg
     3961 gatacgcccg gcgccgcggt catccggctg gatcccggcc tggccttcgg ctccggcacg
     4021 cacccgacca cggcgctgtg cctgcgctgg ctcgatgggc tggcgacgga gggcgcgttg
     4081 cagggccgcg acgtgctcga cttcggttgt ggcagcggca tcctggcgct ggccgcgatg
     4141 aagctcggcg ccgcgcgcgc catcggcgtc gacaacgatc cgcaggcgat catcgccacc
     4201 gcagacaacg ccgaacgcaa cggcgtccgc atcgatacct tcatgcccgc ggacgaaccg
     4261 gccgcgacct atccggtggt cgtcgccaac atcctcgcgt ccgcgctgga tgcactggcg
     4321 gagacgctgg cggcgcgcac cgcgcccgcg ggccgcatcg cgatgtcggg gatcctggcg
     4381 ggacaggaag acgatctgct gctccgcttc gccccgtggt tcgacgccct gcgcgtggcg
     4441 cgcgaggacg actggatgcg catcgagggc gtgcgcaacg cgcggccggt gagcgcatga
     4501 gccggacccc gagtttcgcc ctgcgcgatg cgcccgccgc cacgcgccgc cgtggcgaat
     4561 ggctggcact cgcggtgctc gtgctggcgt tcgcaggcca attgttcttc ggcgaacgcg
     4621 ccgtgctggc cgccaacgcg cagtggcggc cgctggtcgt ggccgcctgc gacgtcgccc
     4681 gttgcagcct gccgccgtgg cgcgaaccga cggcgttcac gatgctggcg cgcgacgtca
     4741 tcgcggtgcc gcaacatccc ggggtgctgc gcgtgcaggc cagtttccgc aacgacgcgc
     4801 gctgggcgca ggcgtggcca gccctggtcc tcaccttgtc ggatgcggac aatcgcaccc
     4861 tgggcgcgcg ccgcttcctg ccgcgcgatt acctgggcga tgcgccgtcc tcgacgcttc
     4921 tggcaccggg acaggcctcg caactggcgt tcgacatcgt cgagccggcg ccgaacgtgg
     4981 ttgccttcga cttcaggttc gattgagaag gttcgactga gcggcgaccg cgacggactg
     5041 cctcggcgca cgcgacattt ttttcgcgat gaatgcggcg acgcactcac gcctcgccat
     5101 ccttcgcgct agactcgctc ccccgcatcg aggccaagcc gatgcggccg caccgagggg
     5161 ctcccgcttg aacgtttccg ccgaccgcca ggacgccagc cgcgcgccgt tgcgcgacca
     5221 tgtcgccacg tccgtgcgcc gctacctgcg cgacctcgac ggctgcgaca ccgacgacct
     5281 ctacaacatc gccctgcgcg agctggaaat cccgctgttc gcggaagtgc tgcgccactg
     5341 cgacggcaac cagagccgcg ccgcgtcgat gctgggcatc catcgcgcca cactgcgcaa
     5401 gaagctcaag gattacgggc tgtcgtcctg acgccatcga tatccgcttt tgtgggaggg
     5461 gcttcagccc ctcccacaag ggcaccggca cgctcgcacg gcctataatc cgcgttcccc
     5521 accgcgcgac ccccgcatga ccaccaacct gtccgaaggt ccgtccgtgg ccattcgccg
     5581 cgccctgctg tccgtgtccg acaagaccgg cctgctcgac ctcgcgaaag cgctcgcctc
     5641 gcgcggcgtg gagttgctct ccaccggcgg caccgccaag gcgttgcgcg atgccggcct
     5701 ggcggtcaag gacgtcagcg agcacaccgg cttcccggaa atgatggacg ggcgggtcaa
     5761 gaccctgcac ccgaaggtgc atggcggcct gctggggcgc gcaggcatcg acgatgcggt
     5821 gatggccgag cacggcatcg acgcgatcga cctgctggtg ctgaacctgt atccgttcgc
     5881 gaaggtgtcg atggatccat cgagcacgtt cgaggacgtg atcgagaaca tcgacatcgg
     5941 cggcccggcg atgctgcgca gcgcggccaa gaatttcgcc cgcgtcgcgg tggccacgga
     6001 tccggcgcag tacgccggca tcgtcgacga gttgaatgcc agcgccggca cgctgtcggc
     6061 gaagacccgc ttcgcgttgg cggtggccgc gttcaacaac gtcgccttct acgacgcctg
     6121 catcagcaac ttcctgtccg ccaagcagga ggacggcagc catgcgcagt tctccgcgca
     6181 ggccaacggc aatttcgtca aggtgatgga cctgcgttac ggcgagaacc cgcaccagca
     6241 ggcggcgttc taccgcgacc tgtggccggc acccggctcg ctcgccacct tcacccagtt
     6301 gcaaggcaag gagctcagct acaacaacat cgccgacagc gacgccgcat gggaatgcgt
     6361 gcgccagttc gatgcgccgg cctgcgtcat cgtcaagcac gccaatccct gcggcgtggc
     6421 ggtcggcgcg gcctgcggcg atgcctacga gctcgcctat gccaccgacc cgacgtcggc
     6481 cttcggcggc atcctcgcgt tcaacacgaa actcgatgcg gcgacctgca aggcgatcct
     6541 cgaccgccag ttcgtcgagg tgctgatcgc ccccgactac gaggacggcg cgctcgagta
     6601 cgcccgcaag aaggccaacg tgcgcgtgct gcgcatcccg atggcgaagc cctcgcccgg
     6661 cttcatcgac accaagcgcg tcggctccgg gatgctgatg cagaccgccg atgaccgcgt
     6721 gatcacgcgc gacgacctca aggtcgtgac gaaactggca ccaaccgagg cgcagttcgc
     6781 cgacctgctg ttcgcctgga aggtggcgaa gttcgtcaag tcgaacgcca tcgtgtacgc
     6841 caaggaaaac cgtacgatcg gcgtgggcgc cgggcagatg agccgggtgt attccgcgcg
     6901 catcgcaggc atcaaggcgc acgatgccaa cctgcaggtg gaaggctcgg tgatggccag
     6961 cgatgcgttc ttcccgttcc gcgacggcat cgacgccgcc gccgaagccg ggatcaaggc
     7021 cgtgatccag ccgggcggtt cgatgcgcga tgccgaagtg atcgccgccg ccgacgaaca
     7081 cggcctggcg atggtgttca ccggc
//
LOCUS       DAOOCX010000111         6997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_10118_length_6997_cov_8.537597, whole genome shotgun sequence.
ACCESSION   DAOOCX010000111 DAOOCX010000000
VERSION     DAOOCX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10118_length_6997_cov_8.537597"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..131)
                     /locus_tag="PK399_09705"
                     /pseudo
     CDS             complement(1..131)
                     /locus_tag="PK399_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
     gene            complement(248..1276)
                     /locus_tag="PK399_09710"
     CDS             complement(248..1276)
                     /locus_tag="PK399_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HPW13434.1"
                     /translation="MIIRARAPLRLGLAGGGTDVAPYCDLHGGCVMNAAIDKYAYATV
                     DAGSDGLVVFEALDRGARVAVAPAELDANAGPVQLLAGAYLRISHDYLGGERPPLRIR
                     TWSDAPPGSGLGSSSTLVVALVAALAEYFSLALGEYEIAHLAYDIERVDLGLAGGKQD
                     QYAAAFGGFNFMEFHAGDRVIVNPLRIKDWVSAELEASLLLYFTGVSRESARIIDEQA
                     RNAASGAVNPIEAMHQLKAEAVQMKEHLLRGDLPGFARTLQQGWQAKKRMASSISNPM
                     IDALEALAMDNGAVATKVSGAGGGGFMMFVCDPVDRVRLSAALSTQGGSLLDFHFNPG
                     GATAWRTP"
     gene            complement(1273..5091)
                     /locus_tag="PK399_09715"
     CDS             complement(1273..5091)
                     /locus_tag="PK399_09715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW13435.1"
                     /translation="MALLVLGMHRSGTSALTRVLNLLGVALGDDLMPPGPDNPLGFWE
                     HQGIVSVHEALFAELDRRWDDPRPMPDGWLDSDAAREAGEAITAILHRDFAGTPAWAV
                     KDPRLCRLIPLWRRVLGGLGIEPRVLLVSRHPREVAGSLLRRDGLPVVIGELLWARYL
                     LDAVRGSAGCRRDMIAYDDLLDDWRASVRRIDQSLALDLVADAEQARAIDAFLSPQLR
                     HHHASASALDGSLAPLAPLAAGALDASGVDTAEQRFSTGIATALPLVDAYAGLLARSR
                     DEVAALRRITDAMRGELDEQGRWAAQLDEALHAMRDLQGAQVGEHEAVVGWATSLRDE
                     LLKLQGLYRQAEDDRDEKADWARALQAELASLQGVYRQAEADRAEKIAWATSLQHELA
                     SLQALYRQADADREAKTSWAMSLQGELAALQSLYRQLEADRDTKTQWAQALGRELARS
                     RLDYERVDADRLEKLAWAQSLDAELADSRGRMAALEATVARQERQEHEMRDQLDKMAG
                     ELTHLQASLAREEAALGRERAARQSLERYADQMEQVLGGVLGSSSWKLARPLRRLLAR
                     LRGSGAEPTLPQRPRALALPAANPADAVAAPLEGDPGLATTGDAIESAATREPANPFA
                     GLAFEAVPAPRVSIVIPTYGKADYTARCLHSLQQAGAHASFEVLVLEDASGDPAMTPL
                     RDVPGLRYHENPRNLGFLRSCNQALQLARGEYICLLNNDTEVTRDWLDALLDVFAIHP
                     DAGVAGSMLVYPDGRLQEAGGIVWRDASAWNYGRLGDPDANEFNYVRRADYCSGAALL
                     LPRALFERLGGFDERYAPAYCEDSDLCFRVREAGLQVYYTPFSRVVHHEGVSHGTDTG
                     GGIKAYQLANQQTLRTRWQDALQAHYPNGEQVTRARDRAWDRPMVLVVDHYIPQPDRD
                     AGSRTMLAFIRALLDAGAVVKFWPDNLYYDPNYAPQLQAMGVEVFHGVRWRDGIGAML
                     REYGDVFDAVLLSRPDVADAHLDAIRACSRARVVYYGHDLHFRRMRDQAALLPEGDDR
                     DALLRAAAAMERRERTIWQRCDGVLYPSEDEATQVRTIEPAADACAITPYAYERFGDD
                     AVLQQREHLLFVAGFAHPPNVDAARWLVDSIMPRVWERRPEARLSLVGSNPTAEVLAL
                     AGERVEVTGFVDDAELQRRYAHARVAVVPLRYGAGVKSKVVEALQQGLPLVTTSVGAQ
                     GLPDVETTCAVADDEAALAGHLLRLLDDDAAWARQSRLGAAYARAHFSRDTLRTQLAS
                     ALAIAAEPAQ"
     gene            complement(5156..>6997)
                     /locus_tag="PK399_09720"
     CDS             complement(5156..>6997)
                     /locus_tag="PK399_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPW13436.1"
                     /translation="MNDLVTLRTSSPQSPQNFYRLAVQVLRNYGVATEYSSGMVMFSR
                     AQGTAGTVPPLIVSGRTLPEVPISHRPLFQLVELQSIRVNDVISLLNVAFKSDALTIQ
                     SDSARNAVILMGRPEMVRQALDAIRVFDRPSMRGRQSARLEPAFVTAEELAKRLVEVL
                     GAEGYGASLYSGSGAGGGSILVLPLTAANTVLVFAGDKAVLEHTVEWARSIDRPNPTA
                     GTDGLFYYMVKNTRADEIVKTITGVRTGSTTRNSSRDAVDGSGVGAPPAAPAAGSATL
                     TAAASAASGGDLSGGKLTLDETRNALIYQGSATDWGRLLPLIQQMDKAPRQVMIEVTV
                     AEVTLTNDQEFGVAWLANNSDLGKFNGNISSGVLSSATSGAGLNYLLDIGGQTRASLK
                     ALASQDRISVLSTPRLMVKSGEEANLDVGTEVPTISSQATSGITTGGNTGIIQTVQYR
                     KTGILLSIKPVVYSNDRVDIELRQEVSEALPVGADSTVNSPAIFNRSYSTSLSLKDGS
                     AILIAGLMSQRQTNGNSGVPFLKDVPLLGNLFKTQKQGRNKTELVLMIVPYIIETDTQ
                     AEELTRSLSQRFELLELPLSTTQPREIRVEPSVPVDGKGQFPAKRTQ"
BASE COUNT         1086 a         2458 c         2338 g         1115 t
ORIGIN      
        1 ccatggtcat caccggatcg tcggcacggg ccgaaccggc tgaccagctc gccggcccag
       61 tgctgcgcgt cggcggcgct gccaccattg ccgcagaaca gcagcttgcg accgttgcgc
      121 aatgcgtcga tgcaccgcgc cacggcatcc tcgagttgcg catgcagggc gctgtcctcc
      181 gacaatgcct gcatgttcgc cagcgccttg tcgaattcgc catggatgac ttgctggatc
      241 gcgtcgctca tggcgtcctc catgcggtcg cgccgccggg gttgaaatgg aaatccaaca
      301 ggctgccgcc ctgtgtcgac aatgcggccg aaaggcgcac tcgatcgacc gggtcgcaga
      361 cgaacatcat gaaaccaccg ccgcccgcgc cggacacctt ggtcgcgacc gcgccattgt
      421 ccatggccag cgcttccagc gcgtcgatca tcgggttgct gatgctgctg gccatgcgct
      481 tcttcgcctg ccagccctgc tgcaatgtgc gtgcgaaacc gggcaggtcg ccgcgcagca
      541 ggtgctcctt catctgcacg gcctcggcct tgagctggtg catcgcctcg atcggattca
      601 ccgcgccgct ggcggcattg cgcgcctgct cgtcgatgat ccgcgccgac tcgcgcgaca
      661 cgccggtgaa gtacagcagc agggatgcct cgagttccgc cgacacccag tccttgatcc
      721 gcagcggatt gacgatcacg cggtcgccgg cgtggaactc catgaagttg aacccgccga
      781 acgcggcggc gtactggtcc tgcttgccgc cggccaggcc gaggtcgaca cgctcgatgt
      841 cgtaggcaag atgggcgatc tcgtattcgc ccagcgccag cgagaagtac tcggccagcg
      901 ctgccaccag cgcgaccacc agggtcgatg acgagccgag gccggatccc ggcggcgcgt
      961 cggaccaggt gcggatgcgc agcggcgggc gttcgccacc cagatagtcg tggctgatgc
     1021 gcaggtaggc acccgcgagc aactgcaccg gcccggcatt cgcgtccagc tcggcaggcg
     1081 caacagcgac gcgcgcgccg cgatccagcg cctcgaagac caccaggcca tcgctcccgg
     1141 catcgacggt cgcgtatgcg tatttgtcga tggcggcgtt catcacgcag ccgccgtgca
     1201 ggtcgcagta cggggcgaca tcggtaccac cgccggccag gccgaggcgc agcggcgcgc
     1261 gcgcgcggat gatcattgcg cgggctccgc ggcgatggcg agcgcggacg ccagttgcgt
     1321 gcgcagcgtg tcgcgcgaga aatgcgcgcg cgcatacgcc gcgccgaggc gggattggcg
     1381 cgcccatgcc gcgtcgtcgt ccagcaagcg caacagatga ccggcgagcg cagcctcgtc
     1441 gtcggcgacg gcgcaggtcg tttcgacatc gggaagccct tgcgcgccga ccgaggtggt
     1501 caccagcggc aagccctgct gcaaggcttc caccaccttg ctcttcaccc ccgcgccata
     1561 gcgcagcggc accaccgcaa cgcgcgcatg cgcatagcgg cgctgcagtt cggcatcgtc
     1621 gacgaaaccg gtgacttcga cgcgttcgcc ggccagcgca agcacttccg cggtcgggtt
     1681 ggagccaacc agcgacagcc gcgcttcggg gcggcgttcc cacacgcgcg gcatgatcga
     1741 gtcgaccagc cagcgcgcgg catccacgtt cggcggatgg gcgaagccag cgacgaacag
     1801 caggtgttcg cgttgctgca agaccgcgtc gtcgccgaag cgctcgtacg catacggggt
     1861 gatcgcacac gcatccgctg cgggttcgat cgtgcgcacc tgcgtggcct cgtcctcgct
     1921 cggatacagc acgccatcac agcgctgcca gatggtgcgt tcgcgtcgtt ccatcgccgc
     1981 ggcggcgcgc agcaaggcat cgcgatcatc gccctcgggc agcaacgcgg cctggtcgcg
     2041 catgcggcgg aaatgcaggt cgtggccgta gtacacgaca cgcgcgcggc tgcaggcacg
     2101 gatcgcatcg agatgggcat cggccacatc cggccgcgac agcagcaccg catcgaacac
     2161 gtcgccgtat tcgcgcagca tcgcgccgat gccgtcacgc cagcggacgc cgtggaagac
     2221 ttccacgccc atcgcctgca gctgaggcgc gtagttcggg tcgtaataca gattgtccgg
     2281 ccaaaacttc accactgcgc cggcatccag cagggcgcga atgaaggcga gcatggtgcg
     2341 ggagccggca tcgcggtccg gttgcgggat gtagtggtcg accaccagca ccatcggccg
     2401 gtcccaggcg cggtcgcgcg cgcgggtgac ctgctcgccg ttcggatagt gcgcctgcag
     2461 cgcatcctgc cagcgcgtgc gcagggtctg ctggttggcg agctggtagg ccttgatgcc
     2521 gccaccggta tcggtgccgt gcgacacgcc ttcgtggtgg acgacgcgcg agaacggcgt
     2581 gtagtagacc tgcaggccgg cttcgcgcac gcggaagcag aggtcggaat cctcgcaata
     2641 cgccggcgcg tagcgctcgt cgaaaccgcc cagtcgctcg aacaacgcgc gcggcagcag
     2701 cagcgccgcg ccggagcagt aatcggcgcg acgcacgtag ttgaattcgt tggcgtcggg
     2761 atcgcccagc cggccgtagt tccatgccga ggcatcccgc cagacgatcc cgcccgcctc
     2821 ctgcaagcgc ccgtcgggat acaccagcat ggaaccggcc acgccggcat cggggtggat
     2881 ggcgaacacg tcaagcaacg cgtccagcca gtcgcgggtg acttcggtgt cgttgttgag
     2941 caggcagatg tactcgccgc gcgccagttg cagcgcctgg ttgcaggagc gcaggaagcc
     3001 caggttccgc gggttctcgt ggtaacgcag gccgggcacg tcgcgaagcg gggtcatcgc
     3061 gggatcgccc gaggcgtcct ccagcacgag gacttcgaag ctggcatgcg cgcctgcctg
     3121 ctgcagcgag tgcaggcagc gcgcggtgta gtcggccttg ccgtaggtcg ggatgacgat
     3181 gctgacccgc ggcgcgggga ccgcctcgaa ggcaaggcca gcgaacgggt tggccggctc
     3241 gcgcgtcgcc gccgactcga tggcatcccc cgttgtcgcc aacccggggt cgccctcgag
     3301 cggcgcagcg accgcgtccg ccgggttcgc ggcgggcagg gcaagcgcgc gcgggcgctg
     3361 tggcaacgtg ggctcggcgc cactgccgcg caatcgggcc agcaggcgtc gcagcgggcg
     3421 cgccagcttc caactggagg agcccagcac gcctcccagc acctgctcca tctggtcggc
     3481 atagcgctcc agcgattgcc gggccgcgcg ttcgcgtccg agcgccgcct cttcgcgcgc
     3541 aagcgatgcc tgcaagtgcg tcaactcgcc agccatcttg tccagctggt cgcgcatctc
     3601 gtgttcctgc cgttcctgcc gggccaccgt cgcttcaagc gcggccatcc gtcccctgct
     3661 gtccgccagt tccgcatcca gggactgcgc ccaggccagc ttctccaggc gatcggcgtc
     3721 gacgcgttcg tagtccaggc gcgatcgtgc cagctcgcgg cccagagcct gcgcccactg
     3781 cgtcttggtg tcgcgatcgg cctccagttg ccgatagagc gattgcagcg cggccagttc
     3841 gccctgcagc gacatggccc acgaggtctt cgcctcgcga tcggcgtcgg cttgccggta
     3901 caacgcctgc aaggatgcca attcgtgctg caacgacgtc gcccaggcga tcttctccgc
     3961 acgatccgcc tcggcctggc ggtacacgcc ctgcagcgac gccagctccg cttgcaaggc
     4021 acgcgcccag tccgcctttt cgtcgcgatc gtcttccgcc tgccggtaca gcccctgcag
     4081 cttgagcaac tcgtcgcgta gcgatgtcgc ccagccaacc acggcctcgt gttcgccgac
     4141 ctgcgcgccc tgcaggtcgc gcatcgcatg cagggcctcg tcgagttgcg cggcccagcg
     4201 gccttgctcg tcgagttcgc cgcgcatggc gtcggtgatc ctgcgcagcg ccgcgacttc
     4261 gtcgcgactg cgcgcaagca ggccggcata ggcgtcgacg agcggcaagg ccgtggcgat
     4321 gccggtgctg aaccgctgtt ccgcggtgtc cacgccggac gcgtcgaggg caccggcggc
     4381 gagcggtgcc aatggcgcca gggagccatc cagcgcgctc gccgacgcat ggtgatggcg
     4441 cagttgcggg cttaggaaag cgtcgatcgc gcgcgcctgc tcggcatccg ccaccaggtc
     4501 cagcgccagc gactggtcga tgcggcgcac gctggcgcgc cagtcgtcca gcaggtcgtc
     4561 gtaggcgatc atgtcgcgac ggcagcccgc gctgccgcgc accgcatcga gcagatagcg
     4621 cgcccacagc agttcgccga tcacgaccgg caggccatcg cggcgcaaca gcgagccggc
     4681 gacctcgcgt ggatgacgcg acaccagcag cacccgcggc tcgatgccca gcccgcccag
     4741 cacgcgtcgc cacaacggga tcagccggca caggcgcgga tccttgaccg cccaggcagg
     4801 cgtgccggcg aaatcgcgat gcaggatcgc ggtgatcgcc tcgcccgctt cgcgcgccgc
     4861 gtcgctgtcc agccagccgt caggcatcgg ccgcgggtcg tcccagcgcc ggtcgagctc
     4921 ggcgaacagg gcctcgtgca cggagacgat gccctggtgc tcccagaagc caagcgggtt
     4981 gtccgggccg ggcggcatca ggtcatcgcc cagcgccaca cccaagaggt tgagcacgcg
     5041 ggtcagcgcc gaggtgccgc tgcgatgcat gcccagcacc agcagcgcca ccggcgcact
     5101 cgccgtggcc ctcccctgaa cctgtcttgc catgcccctg ccgcctgcgg tcaactcact
     5161 gcgtgcgctt cgcggggaac tggcccttgc cgtccaccgg caccgacggc tcgacccgga
     5221 tctcgcgcgg ctgggtggtc gacagcggca gttccagcaa ctcgaagcgc tggctgagcg
     5281 agcgggtcag ttcctccgcc tgggtatcgg tttcgatgat gtagggcacg atcatcaaca
     5341 ccagttcggt cttgttgcgc ccctgcttct gggtcttgaa caggttgccc agcaagggca
     5401 cgtccttgag gaacggcaca ccgctgttgc cattggtctg ccgctgcgac atcaggccgg
     5461 cgatcaggat cgcgctaccg tccttgaggc tgaggctggt gctgtaggag cggttgaaga
     5521 tggccggcga attcaccgtg gagtccgcgc cgaccggcag cgcctcgctc acttcctggc
     5581 gcaattcgat atccacccgg tcgttggaat acaccaccgg cttgatgctc agcaggatgc
     5641 cggtcttgcg gtactgcacg gtctggatga tgccggtgtt gccaccggtc gtgatgccgg
     5701 aggtggcctg cgagctgatc gtgggcactt cggtgccgac gtcgaggttg gcttcctcgc
     5761 cactcttcac catcaggcgg ggcgtcgaca gcacgctgat ccggtcctgg ctggccagcg
     5821 ccttcagcga ggcccgggtc tgcccgccga tgtccagcag gtagttcaac ccggcaccgc
     5881 tcgtcgcgct actcaggacg ccggaactga tgttgccgtt gaacttgccg aggtccgagt
     5941 tgttggccag ccatgcaacc ccgaattcct ggtcgttggt caaggtcact tccgccacgg
     6001 tgacttcgat catcacctgc cgcggcgcct tgtccatctg ctggatcaac ggcaacagcc
     6061 tcccccagtc ggtcgccgac ccttggtaga tcaacgcgtt gcgcgtctcg tcgagggtca
     6121 gcttgccgcc actcaggtcg ccaccggacg ccgcgctggc cgcggcggtg agcgtggccg
     6181 aacccgcggc gggcgcagcg ggcggcgcgc cgacgccgct gccgtcaacc gcatcgcgcg
     6241 aggaattccg cgtcgtcgag ccggtgcgca cgccggtgat cgtcttcacg atttcgtcgg
     6301 cgcgggtgtt cttgaccatg tagtagaaca ggccgtcggt gcccgcggtg gggttcggtc
     6361 ggtcgatgct gcgcgcccat tcgaccgtgt gctccagcac ggccttgtcg ccggcgaaca
     6421 ccagcacggt gttggcggca gtcagcggca gcaccaggat cgaaccgcca ccggcaccgc
     6481 tgccgctgta cagcgaggcg ccataacctt ccgcccccag cacttcgacc aggcgcttgg
     6541 cgagctcctc agcggtgacg aatgccggtt ccagccgcgc gctctggcgt ccccgcatcg
     6601 acggacggtc gaacacccgg atcgcatcca gcgcctgccg caccatttcc ggacgaccca
     6661 tcaggatgac cgcattgcgg gcgctgtcgg actggatggt gagcgcgtcc gacttgaacg
     6721 caacattgag caggctgatc acgtcgttga cgcggatcga ctgcaattcc accaactgga
     6781 acagcgggcg atggctgatc ggcacttccg gcagcgtgcg gccgctcacg atcagcggtg
     6841 gcacggtccc ggcggtgccc tgcgcgcggc tgaacatgac catgccggag ctgtactccg
     6901 tcgccacccc atagttgcgc agcacctgca cggcgagccg gtagaaattc tgcggcgatt
     6961 gcgggctcga ggttcgcagg gtcaccaggt cgttcat
//
LOCUS       DAOOCX010000112         6951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_10198_length_6951_cov_9.391096, whole genome shotgun sequence.
ACCESSION   DAOOCX010000112 DAOOCX010000000
VERSION     DAOOCX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10198_length_6951_cov_9.391096"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PK399_09725"
     CDS             <1..1249
                     /locus_tag="PK399_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPW13437.1"
                     /translation="WDQSLASLKKLCIAHGVQLIAIGNGTASRETDKLAGDLLKLVPE
                     LKAQKIVVSEAGASVYSASELAAKEFPNLDVSLRGAVSIARRLQDPLAELVKIEPKAI
                     GVGQYQHDVDQYRLARALDARVEDCVNAVGVDVNTASSALLARVSGLSATVAENIVVY
                     RDAHGAFASRKALLKVPRLGDKTFEQCAGFLRIADGEQPLDASSVHPEAYPVVERILA
                     AGGKQVKQVIGDTAFLRSLKAEQFTDDTFGLPTVRDILKELEKPGRDPRPEFKAATFA
                     DGIEDIRDLRPGMVLEGVVSNVAAFGAFVDIGVHQDGLVHISALSDTYVKDPREVVKA
                     GDIVKVKVLEVDVARKRIALTRRMGDEPGQATSRPPGGERDARRDGARGNDRNRPHVP
                     RPQQQAAPFNSAMADALKGLKRG"
     gene            complement(1375..1911)
                     /locus_tag="PK399_09730"
                     /pseudo
     CDS             complement(1375..1911)
                     /locus_tag="PK399_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
     gene            complement(1946..2797)
                     /gene="purU"
                     /locus_tag="PK399_09735"
     CDS             complement(1946..2797)
                     /gene="purU"
                     /locus_tag="PK399_09735"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HPW13438.1"
                     /translation="MRHDHILTLSCPDRTGIVHRVSGCLFDAGCNILDAQQFGDGETG
                     RFFLRVHFDAPAANAIEAFRASFAALADDFGMDWQLHDARRRARLLMLVSKQGHCLND
                     LLFRTHSRQLRADIVAVASNHQDFSALASSYGVPFHHLPVDAGNREAQEQAIIDLAKR
                     EQVDLVVLARYMQILSPRLCEALAGRAINIHHSFLPSFKGAQPYHQAHARGVKLIGAT
                     AHYVTPDLDEGPIIEQDVARVDHAMTPRDLVRMGSDIESQVLARAVRHHIEHRILPNG
                     HRTVVFR"
     gene            complement(2837..4099)
                     /locus_tag="PK399_09740"
     CDS             complement(2837..4099)
                     /locus_tag="PK399_09740"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW13439.1"
                     /translation="MTDSTLPAMSRRHLLAGGIGLASLAAWPARVNAAQPWNDDADWI
                     PGDALLQDLPRQMQALGVPGIGIAVVEAGRLAWSRGFGVTHAERGTPVEDGTLWEAAS
                     LSKPVFAYMVMQLVDRGELALDAPLVKYVRPAYLGQSPWIERIAVRDVLRHTTGLPNW
                     RKDPANEWLVPAVAPGTRVDYSGEAFVWLQLAVEAVTGESLDQAMHRLLFAPAGMHDS
                     SYGWDAALAARSVYGHRAPDDTQPGMPAQGLREAWDAAQPVADQWGKPLSSWRYADAE
                     RALPQARANAKPGVVNWPSDILANSAASLRCTVQDYARLMALVVHAAPAAWELKPATR
                     AAMLASQIALPGRWTEKGLGWNLEATRIGPVLYHSGSNAGVFKTFALGDAARQRAIVI
                     MTNAANGNVLYRRIVRASTGLDLLAFDL"
     gene            4187..4384
                     /locus_tag="PK399_09745"
     CDS             4187..4384
                     /locus_tag="PK399_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HPW13440.1"
                     /translation="MTSPLSSADPDPRPLPPERPLPSDCCDSGCAVCVHDLYAEEVMA
                     HRQALAAWMLRHPDTEATATQ"
     gene            complement(4394..5413)
                     /locus_tag="PK399_09750"
     CDS             complement(4394..5413)
                     /locus_tag="PK399_09750"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HPW13441.1"
                     /translation="MSAQRRQLLLDPGFELTLRPMRYPRFYEMYRDAIRNTWTVEEVD
                     FSLDTSDLKTKFGPAERHLVERLIAFFATGDSIVSNNLVLNLYQHINAPEARMYLSRQ
                     LYEEALHVQFYLTLLDTYLPDPNERARAFAAVENIPSIRRKAEFCFKWIDSLQGIARI
                     ETRAQRRQFLLNMICFAGCIEGLFFFGAFAYVYYLRSRGLLHGLASGTNWVFRDESAH
                     MAFAFEVIRTAREEEPDLFDEEMKAQVVQMMSEAVDCEVAFAEDTLSGGVAGLSIRDM
                     RHYLEYCADQRMVQLGMPRHFGSRNPFPFMDLQDVQELTNFFERRVSAYQVGVQGEVS
                     FDHAF"
     gene            complement(5410..>6951)
                     /locus_tag="PK399_09755"
     CDS             complement(5410..>6951)
                     /locus_tag="PK399_09755"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HPW13442.1"
                     /translation="IVPWLKTLDSSVAAVNQGGKRKGAACVYLEPWHADVEEFLELRD
                     NTGDEARRAHNLNLANWIPDLFMQRVEADADWSLFDPRVVPEFTDLFGGDFERAYAQA
                     EAQGKAVKTIKARELYARMMRTLAQTGNGWMTFKDTCNRASNQTLRPGNVIHLSNLCT
                     EILEVTSNDETAVCNLGSINLARHFDDDGLFDFDKLAETVRLAVRQLDRVIDLNFYPI
                     ETARRGNLRWRPVGLGCMGLQDVFFKLRLPFDGDEARALSARIAETIYFHALETSCEL
                     AEERGQHPSFADTRAARGELQFDAWNVVPANSERWDALRQRIRLHGLRNSLLVAIAPT
                     ATIASIAGCYECVEPQVSNLFKRETLSGDFLQVNRYLVEELKKLGLWTPEIRDAIKQA
                     EGSVQGVVQIPETLRHVYRTAWEIPMRALIDMAAGRGAFIDQSASLNLFMESPNIGAM
                     SSMYMHAWKQGIKTTYYLRSRPATKIAKTTLAGATVAAPKPEVAPVDAIACSLENPET
                     CEACQ"
BASE COUNT         1207 a         2297 c         2323 g         1124 t
ORIGIN      
        1 gtgggaccag tcgctcgcct cgctgaagaa gctatgcatc gcccacggcg tgcaactgat
       61 cgcgatcggc aacggcaccg cctcgcgcga gaccgacaag ctggccggcg acctgctcaa
      121 gctggtgccg gaactgaagg cgcaaaagat cgtggtcagc gaggccggcg cttcggtgta
      181 ttcggcatcg gaactcgcgg cgaaggaatt ccccaacctc gatgtgtcgc tgcgcggcgc
      241 ggtgtcgatc gcgcgccgcc tgcaggaccc gctggcggaa ctggtcaaga tcgagcccaa
      301 ggcgatcggc gtgggccagt accagcacga cgtcgaccag taccggcttg cgcgcgcact
      361 cgacgcaagg gtcgaggact gcgtgaacgc ggtcggcgtg gacgtgaaca ccgcctccag
      421 cgccttgctg gcgcgcgtct ccggcctgtc cgccacggtt gccgagaaca tcgtggtgta
      481 tcgcgacgca cacggcgcat tcgcctcgcg caaggcgttg ctgaaggtgc ctcgcctggg
      541 cgacaagacc ttcgagcagt gcgccggctt cctgcgcatc gccgatggcg agcagccgct
      601 ggatgcatcg tcggtacatc cggaagccta tccggtggtc gagcgcatcc tcgctgctgg
      661 cggcaagcag gtgaagcagg tcattggcga cacggccttc ctgcgctcgc tcaaggccga
      721 gcagttcacc gacgacacct tcggcctgcc gaccgtgcgc gacatcctga aagagctgga
      781 aaagcccggc cgcgatccgc gcccggagtt caaggccgcg accttcgccg acggcatcga
      841 ggacatccgg gacctgcgcc ccggcatggt gctggaaggc gtggtcagca acgtcgccgc
      901 cttcggcgcc ttcgtcgata tcggcgtgca ccaggacggc ctggtccata tctccgcgct
      961 gtccgatacc tacgtcaagg atccgcgcga ggtggtcaag gccggcgaca tcgtcaaggt
     1021 caaggtgctg gaggtcgacg tggcgcgcaa gcgcatcgcc ctgacccggc gcatgggcga
     1081 cgaacctgga caggcgacgt cacgcccacc aggcggcgaa cgcgacgcgc gacgcgacgg
     1141 cgcgcgtggc aacgatcgca accgcccgca tgtgccgcga ccgcagcagc aggctgcacc
     1201 gttcaacagc gcgatggccg acgcgttgaa gggcttgaag cgcggctgag gtcgcgctca
     1261 gtctggatcg acggcgttcg ccgatgcgcc gccgcccatc gcgacggtgc gccgctggtc
     1321 gcggctgttc attggctccg cggcctgcag ggcgacgtcg ggcgcacccg gctcccagcg
     1381 gcgcacggcg cgctggaaga acatgctgtt gggaacccgc agcaggctgt ggccaccgtg
     1441 ggcatgggtc tcgcgcaggg tgacgtagac cagggtgatg tccatgacct ggccacgcag
     1501 gcccggccgc tcgccgcttt ccagcagttc cacgtggtcg tgcaggcgga acgggcggct
     1561 ggtcatgatc aggaacgcgc agaacacgtt ggtcagcacg ctccacgcag cgaagaacgc
     1621 caccgccgcc accgtggcga agccggtgaa cgcgccccac agcacgccgg cggacacgcc
     1681 gaccacgtgc aggatcgcca gcagggcggc gaatccgacc aggaagccgc tcacccggcg
     1741 cacgcccatg ctgacttcca gcggcaggtc gtagcgccgc gccaaccgtg cgaccagacg
     1801 cctcagcagc caacggacgg cggacgcgac cacgaggatc aacaacacct gcaggacgat
     1861 cgccacttcg gcgaaccacg gacgcaacca gtcgggcaaa tacgtgctca tgccgcgcat
     1921 ggtaaccgcg ggtggcgcgg cggcttcagc ggaacaccac cgtgcgatgc ccattcggca
     1981 ggatgcggtg ttcgatgtgg tggcgcaccg cgcgggcgag cacctgcgat tcgatgtcgc
     2041 tgcccatgcg gaccaggtcg cgcggggtca tcgcatggtc cacgcgggcg acgtcctgtt
     2101 cgatgatcgg gccttcatcc aggtccggcg tcacgtagtg cgcggtggcg ccgatcagct
     2161 tgaccccgcg cgcgtgcgcc tggtggtatg gctgcgcgcc cttgaagctg ggcaggaagc
     2221 tgtgatggat gttgatcgcg cggccagcca atgcctcgca caggcgcggc gagaggatct
     2281 gcatgtagcg cgccagcacc accaggtcga cctgttcgcg cttggccagg tcgatgatcg
     2341 cctgctcctg cgcctcgcga ttgccggcgt ccaccggcag gtggtggaac ggcacgccgt
     2401 aagaggacgc aagcgcggaa aagtcctggt gattcgacgc caccgcgacg atatcggcgc
     2461 gcagttgccg gctgtgggtg cggaacagca ggtcgttgag gcaatggcct tgcttgctca
     2521 ccagcatcaa caatcgcgcg cggcgacgcg catcgtgcaa ctgccagtcc atgccgaagt
     2581 cgtcggccag cgcggcaaag ctggcgcgga acgcctcgat cgcgttcgcc gcgggcgcat
     2641 cgaaatgcac gcgcaggaag aagcgcccgg tttcgccgtc gccgaactgc tgggcatcca
     2701 ggatgttgca gccggcgtcg aacaggcagc cggagacgcg atggacgatg ccggtgcgat
     2761 ccgggcagga cagggtcagg atgtggtcgt ggcgcatggg ggatgcggtg ggcgtcgcgc
     2821 ggggcgcgcc actatgtcac aggtcgaatg ccagcagatc cagccctgtc gaggcgcgca
     2881 cgatgcgccg gtacagcacg ttgccgttgg cggcgttggt catgatgacg atggcccgct
     2941 ggcgagcggc atcgcccagg gcgaaggtct tgaagacgcc ggcgttgctg ccggagtggt
     3001 acagcaccgg gccgatccgg gtcgcttcga ggttccagcc caggcccttc tccgtccatc
     3061 gaccgggcaa tgcgatctgc gatgccagca ttgcggcacg ggtcgcgggt ttcaattccc
     3121 acgcggcagg cgctgcatgc accaccaatg ccatcaggcg cgcgtagtcc tgcaccgtac
     3181 agcgcaggct ggctgccgaa ttggccagga tatcgctggg ccagttcacc acgcccggct
     3241 tcgcattcgc gcgggcctgc ggcaacgcgc gttcggcatc cgcatagcgc caagaagaga
     3301 gtggcttgcc ccactgatcc gcgacgggct gtgccgcgtc ccaggcctcg cgcaatccct
     3361 gtgccggcat gcccggctgg gtgtcatccg gtgcgcgatg accgtaaacc gagcgcgccg
     3421 ccaatgcggc atcccagcca taactgctgt cgtgcatgcc ggcgggcgcg aacagcaggc
     3481 gatgcatggc ctggtccagg ctttcgccgg tgaccgcttc caccgcgagt tgcagccaga
     3541 cgaaggcttc gccggaatag tcgacgcgcg tgcccggcgc cacggcaggc accagccatt
     3601 cgttggccgg atccttgcgc cagttcggca acccggtcgt gtgacgcagc acgtcgcgca
     3661 cggcgatgcg ctcgatccac ggcgattggc cgaggtaagc cggccgtacg tatttcacca
     3721 gcggcgcatc cagcgccagc tcgccgcgat cgaccagttg catcaccatg taggcgaaca
     3781 ccggtttgct cagggatgct gcttcccata gcgtgccgtc ttcaaccggc gtaccgcgtt
     3841 ccgcatgggt cacgccgaag ccgcgcgacc atgcgagccg gcccgcctcc accactgcga
     3901 tgccgatgcc gggcacgccc agcgcctgca tctgccttgg caagtcctgc aggagcgcat
     3961 cgccgggaat ccaatcggca tcgtcattcc agggctgcgc cgcattgacc cgtgcgggcc
     4021 aggccgccaa tgacgccaat ccaatgccgc cggccagcag gtggcgacgc gacattgcag
     4081 gcagtgtgga atcggtcatg ccgcattgga gcggttcgtc acggatgccg caagcgccca
     4141 cgccaacctg catggataat catcgccaca ccaacgcacc gatcgcatga ccagcccgct
     4201 ctccagcgcc gatcccgacc cgcgtccgct gccgcccgaa cgcccgctgc cctcggactg
     4261 ctgcgacagc ggttgcgcgg tctgcgtgca tgacctgtac gcagaggaag tgatggcgca
     4321 tcgccaggcg cttgcagcgt ggatgctgcg gcacccggat acggaggcca cagcgacgca
     4381 atagcagggc cgatcaaaac gcgtggtcga agctgacctc gccctgaacg ccgacctgat
     4441 acgcggacac gcggcgctcg aagaagttgg tcagctcctg cacgtcctgc aggtccatga
     4501 acgggaacgg gttgcgtgac ccgaagtgcc gtggcatgcc caactggacc atgcgctggt
     4561 cggcgcaata ttccaggtag tggcgcatgt cgcggatcga caggccggcc acgccgccgc
     4621 tcagggtgtc ttcggcgaag gcgacttcgc agtcgaccgc ttcggacatc atctgcacga
     4681 cctgcgcttt catctcctca tcgaacaggt cgggttcttc ctcgcgggcg gtgcggatga
     4741 cctcgaaggc gaaggccatg tgcgcgctct cgtcgcggaa aacccagttg gtgccgctgg
     4801 ccaggccatg cagcaaccct cgcgaacgca ggtagtacac ataggcgaag gcaccgaaga
     4861 agaacaggcc ttcgatgcag ccggcgaagc agatcatgtt gagcaggaac tgccggcgct
     4921 gcgcgcgcgt ctcgatgcgg gcgatgccct gcaacgagtc gatccacttg aagcagaact
     4981 ccgccttgcg gcggatcgac ggaatgttct ccactgcggc gaacgccctg gcgcgctcgt
     5041 tcggatccgg caggtacgtg tccagcaagg tcaggtagaa ctgcacatgc agcgcttcct
     5101 cgtacaactg ccgcgacagg tacatgcgcg cctccggcgc attgatgtgc tggtacaggt
     5161 tcaacaccag gttgttggac acgatcgaat cgccggtggc gaagaacgca atcaggcgct
     5221 cgaccaggtg gcgttcggcc ggcccgaact tggtcttcag gtcactggtg tccagcgaga
     5281 aatccacttc ctccaccgtc caggtgttgc ggatggcgtc gcggtacatc tcgtagaacc
     5341 gtggataccg catcggccga agggtgagtt cgaaaccggg gtcgagcagc aattgtcggc
     5401 gttgtgcgct cactggcagg cctcgcaggt ttccgggttt tccagcgagc aggcgatggc
     5461 atcgaccggc gcaacctcgg gtttcggtgc ggcaacggta gcgccggcca atgtggtctt
     5521 ggcgattttc gtcgccgggc gcgaacgcag gtagtaggtg gtcttgatgc cctgcttcca
     5581 ggcatgcatg tacatcgacg acatcgcgcc gatgttcggg ctttccatga acaggttgag
     5641 cgaggccgac tggtcgatga aggcgccgcg accggccgcc atgtcgatca gggcgcgcat
     5701 cgggatctcc cacgccgtgc gatagacatg acgcaacgtc tcggggatct gcaccacgcc
     5761 ctgcaccgag ccctcggcct gcttgatcgc gtcgcgtatt tccggcgtcc acaggccgag
     5821 cttcttcaac tcctccacca ggtagcggtt cacctgcagg aaatcgccgg acagggtttc
     5881 gcgcttgaac aggttggaca cctgcggttc cacgcattcg tagcagccgg cgatcgaggc
     5941 gatcgtcgcg gtcggggcga tcgcgaccag cagcgaattg cgcaggccat gcagcctgat
     6001 ccgctggcgc agggcatccc agcgctcgct gttcgcgggc accacgttcc aggcatcgaa
     6061 ctgcagttcc ccgcgcgcgg cgcgcgtatc cgcgaacgaa ggatgctggc cgcgttcttc
     6121 cgccaactcg caggaggttt ccagcgcatg gaagtagatg gtctcggcga tccgtgcgga
     6181 caacgcgcgg gcctcgtcgc catcaaacgg caggcgcagc ttgaagaaga cgtcctgcag
     6241 gcccatgcag ccaagaccca cgggccgcca gcgcaggttg ccgcggcgcg cggtttcgat
     6301 cggatagaag ttgaggtcga tgacgcggtc gagctggcgc accgcgaggc gcacggtctc
     6361 tgccagcttg tcgaagtcga acaggccgtc gtcgtcgaaa tgccgcgcca ggttgatcga
     6421 acccaggttg cacactgcgg tttcgtcgtt ggaggtgacc tcgaggatct cggtgcacag
     6481 gttggacagg tggatcacgt tgcccggccg caaggtctgg ttgctcgcgc ggttgcaggt
     6541 gtccttgaag gtcatccagc cgttgccggt ctgcgccagc gtacgcatca tccgcgcgta
     6601 cagctcgcgc gccttgatcg tcttgaccgc cttgccctgt gcctcggcct gcgcataggc
     6661 gcgctcgaag tccccgccga acaggtcggt gaattcgggc accacgcgcg gatcgaacag
     6721 cgaccagtcg gcatcggcct cgacccgctg catgaacagg tcggggatcc agttggccag
     6781 gttgaggttg tgggcgcggc gggcctcgtc gccggtgttg tcgcgcaact ccaggaattc
     6841 ctcgacatcg gcatgccacg gctccaggta cacgcaggct gcgcccttgc gcttgccgcc
     6901 ctggttgacc gccgccaccg aggaatccag cgtcttcagc cacggcacga t
//
LOCUS       DAOOCX010000113         6951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_10200_length_6951_cov_7.047129, whole genome shotgun sequence.
ACCESSION   DAOOCX010000113 DAOOCX010000000
VERSION     DAOOCX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10200_length_6951_cov_7.047129"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="PK399_09760"
     CDS             <1..1332
                     /locus_tag="PK399_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW13443.1"
                     /translation="RRLIQLKIQREALKKEKDAESKQRLADLQGQIDVLEREYSDLEE
                     VWKAEKATLQGATKLKEQIEAARLQLEAAQRAQDYARMSEIQYGTLPELEKQLAAAQE
                     VEHKGFTLLQDKVTAEEIAQVVARWTGIPVSKMLEGEREKLLAMESQLHTRVVGQEEA
                     IKVVSDAVRRSRAGLSDPDRPSGSFLFLGPTGVGKTELCKALAEFLFDSTDAMVRIDM
                     SEFMEKHAVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQ
                     VLDDGRLTDGQGRTVDFRNTVIVMTSNLGSHQIQELDGDDSPQAYTQMKAAVMGVVQA
                     HFRPEFINRLDDIVVFHSLDKQQIKSIARIQLRGLEKRLGERGIRIELTDKAFELLAN
                     VGFDPVYGARPLKRAIQQQLENPLAQAILSGQFGNGDTVRVDAEDGKLVFG"
     gene            1463..2341
                     /locus_tag="PK399_09765"
     CDS             1463..2341
                     /locus_tag="PK399_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011348303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPW13444.1"
                     /translation="MKSDTRNAYARRVDAVLSRLQRAIAAGDELPDLGDLAAVAHLSQ
                     FHFHRVWRALTGETLGATVARLRLARALQLLGSAGVAIADVALSVGYDTPQALARACR
                     EQLHASPSELREDADRRAALLATLERPLPASTEPVALQVEVVSLEPFEVVALRNRGAF
                     ADLDQAYGALFEWAAGAGLVESIIGLHGIPRGDHRDVPPGDLEFDCAIRLSSPADPDA
                     PLRLLPVEGGMHARVRHVGGYDRLDDVVDRLLADWLPGSGRSLRDLPIRFEFLDDPET
                     VPEPMLRAEVLVPLAD"
     gene            2406..4052
                     /locus_tag="PK399_09770"
     CDS             2406..4052
                     /locus_tag="PK399_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017173966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPW13445.1"
                     /translation="MSLSALPRAARVVLVLCSVLSPVLANAQVSRHVEVRLDAAAMQP
                     RAAGRLLVFAMPAGMAEAAAKGGKVEEVDASAMAPEAVAIAAMEVPSLAAGASVVLDA
                     DAIAFPQPFSRLPKGDYYLQAVLDANHDYNYGGRGEGDLVSEPTRVRLGKSHRIPLLA
                     LTRVQPARPDPWAVADDAPADVREAMAAARAQTDLLDFASPALSAFWGRDIRMRGWVL
                     LPPGYADAGNAATRYPSAYFTHGFGGSLPGLVRTVASVAAAMREGSMPPMIWVFLDES
                     SATGTHEFADSVNNGPWGHALTSELIPSLEARYRMDGKASGRLLNGHSSGGWATLWLQ
                     VTYPKLFGGTWSTAPDSSDFHDFTGPDLYAPDANVYRRPDGSGWPLVRVDGKVVSTFE
                     QYARQESVKGEVGGQLASFDWVFSPRGADGRPMPMFDRATGKVDPAVVAYWREHYDIA
                     HRLQALWPALKPDLDGKIRVIVGDADTFYLDGAARRLKAVLDGLGAKADFRFLPGKTH
                     FDLYVEGDDRRALLKKIAWEMHAVARPGSAAAPAAIPGAL"
     gene            complement(4060..5751)
                     /gene="ybaL"
                     /locus_tag="PK399_09775"
     CDS             complement(4060..5751)
                     /gene="ybaL"
                     /locus_tag="PK399_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HPW13446.1"
                     /translation="MPHHTSLIALLCVGFVMAFALGMLAQRLKLSPLVGYLLAGVIVG
                     PFTPGFVADQTLAPQLAEVGVILLMFGVGLHFSLRDLLDVKNIAVPGALLQIAFVTSI
                     GTAFGLMLGWETMGSIVFGLSLSVASTVVVMRAMEDRRLLDTRRGRIAIGWLIVEDLA
                     MVLALVLLPAIAGALGAGEKVGTAGILGALGWTLLKVAAFVAFMLVVGRRVIPRVLEH
                     VAGLGSRELFTLCVLAIALGVAFGAAALFDVSFALGAFFAGMLLNESELSHKAAADSL
                     PMRDAFAVLFFVSVGMLFNPSILVDHPLLVVATVAIIVFGNALIGFTVVRLFRLPQLT
                     ALTIAASLSQIGEFSFILAGLGLSQKVLPQQGHDLILAGALLSIIANPLLFAWLDRWQ
                     KRQQPGEAVTQVLADNSEVPEDMGGHAIVVGYGRVGRELSRLLQDRGVPLVLVESDPD
                     RVEQARNRGLVTVRGNAASEAILKATRPDTAQLAILAIPQALEAGEIIARFKAMNPAI
                     SVLARAHSEAEVKHLLEHGADAAVLAERELAYSLADMVMATPPYRPARVEPAAAS"
     gene            5847..>6951
                     /locus_tag="PK399_09780"
     CDS             5847..>6951
                     /locus_tag="PK399_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPW13447.1"
                     /translation="MISFRNFALRRGERLLLSDVDLTLHAGWRVGVIGRNGAGKSSLF
                     AALMREVEADKGDLDLPTKARIASVAQETPHLPDPAIDFVLSGDEAVHAAIQMEANAF
                     ANEDWEAVAEAHHRLEEVNGYDGSARAGRLLHGLGFAASAHETPVSDFSGGWRVRLNV
                     ARALMTPSDLLLLDEPTNHLDLDAVVWMEEWLKRYDGTLLVISHDREFLDNVTTHTLH
                     LHEGKAKLYVGNYTDFERQRAEHLRQQQIAHQKEQAERAHLQSFIDRFKAKASKAKQA
                     QSRMKRLAKLAGTEAVRAERSLHITLPEPLKLPNSLLSLHDVEAGYGADAVILHNVNF
                     GLEAGDRIGLLGPNGAGKSTLVKTLVGELAPMTG"
BASE COUNT         1129 a         2352 c         2414 g         1056 t
ORIGIN      
        1 cgccggctga tccagctcaa gatccagcgc gaggcgctga agaaggagaa ggacgccgaa
       61 tcgaagcagc gcctggccga cctgcaggga cagatcgacg tgctcgagcg cgagtacagc
      121 gacctggagg aagtgtggaa ggccgagaag gcgaccctgc agggcgccac gaagctcaag
      181 gagcagatcg aggctgctcg cctgcaactg gaagcagcgc agcgtgccca ggattacgcg
      241 cgcatgagcg agatccagta cggcaccctg ccggaactgg agaagcagtt ggccgctgcg
      301 caggaagtgg agcacaaggg cttcaccctg ctgcaggaca aggtcactgc ggaggaaatc
      361 gcacaggttg tcgcgcgctg gaccggtatt ccggtgagca agatgctgga aggagagcgc
      421 gagaaactgc tggcgatgga gtcgcagctg cacacccgcg tggtcggcca ggaagaggcg
      481 atcaaggtgg tgtccgacgc cgtgcggcgc tcgcgcgccg gcctgtccga tccggatcgt
      541 ccgtctggct cgttcctgtt cctgggcccg accggcgtcg gcaagaccga gctgtgcaag
      601 gcgctggccg agttcctgtt cgacagcacc gacgcgatgg tgcgcatcga catgagcgag
      661 ttcatggaaa agcacgcggt gtcgcggctg gtcggcgcgc ctccgggcta cgtcggttat
      721 gaagagggtg gttacctcac cgaagccgtg cgtcgccgcc cgtacagcgt gatcctgctc
      781 gatgaagtcg agaaggcgca tccggatgtc ttcaacatcc tgctgcaggt gctggacgat
      841 ggccgcctga ccgatggcca gggccgcacg gtggatttcc gcaacaccgt gatcgtgatg
      901 acgtcgaacc tcggctcgca ccagatccag gagctggatg gcgatgactc cccgcaggcc
      961 tacacgcaga tgaaggcggc ggtcatgggc gtggtgcagg cgcatttccg tccggagttc
     1021 atcaaccggc tggacgacat cgtggtcttc cattcgctgg ataagcagca gatcaagtcg
     1081 attgcgcgca tccagttgcg cggcctcgag aagcgcctgg gcgagcgcgg catccgcatc
     1141 gagctgaccg acaaggcctt cgagctgctg gccaacgtcg gcttcgaccc ggtgtacggc
     1201 gcgcgtccgc tcaagcgtgc gatccagcag cagctcgaga acccgctggc gcaggcgatc
     1261 ctgtccggcc agttcggcaa tggcgatacg gtcagggtcg atgcggagga cggcaagttg
     1321 gtgttcggct gacgcgactt gcaggacacg ccagccgtcc gtggttcgta acccggctcg
     1381 tggattgcgc aagatcggga acgcgggccg ggtgcgacgg gatgcatcgt gctgatgcat
     1441 cctcatcgca ccggagaccg ccatgaagtc cgacacccgc aatgcctatg cccgccgcgt
     1501 cgatgccgtg ctctcccgcc tgcagcgggc gatcgcggcg ggcgacgagc tgcccgacct
     1561 cggtgacctc gccgctgtag cgcacctctc gcagttccat ttccaccgtg tctggcgcgc
     1621 gctgaccggc gagaccttgg gcgccaccgt cgcccgcctg cgcctggcgc gtgcgctgca
     1681 actgttgggg tcggcaggcg ttgcgatcgc cgacgtcgcc ctgtcggtcg gctacgacac
     1741 cccgcaggcg ctggcgcggg cgtgccgcga gcagttgcac gcaagcccca gcgagctgcg
     1801 cgaggatgcc gaccgccgcg ccgcattgct cgctaccctg gaacgtccgc tccccgcttc
     1861 gaccgagccc gtggcactgc aggtcgaagt ggtgtcgctg gagccgttcg aagtcgtcgc
     1921 cctgcgcaac cgcggcgcgt tcgccgacct cgaccaggcc tacggcgcgc tgttcgagtg
     1981 ggccgccggt gccgggctgg tcgagtcgat catcggcctg cacggtatcc cgcgtggcga
     2041 ccatcgcgac gtgccgccgg gcgacctcga attcgattgc gcgatccggc tgtcttcacc
     2101 ggcggatccc gacgccccgc tgcgcctcct gcccgtggag ggcggcatgc atgcccgcgt
     2161 gcgacacgtc ggcggttacg accggctgga cgatgtcgtc gaccggttac tggccgactg
     2221 gctgcccggc agcggccgca gcttgcgcga cctgccgatc cgcttcgagt tcctggatga
     2281 ccccgagacg gtgccggaac cgatgttgcg agccgaggtg ctggtgccgc tggccgacta
     2341 gcccggtgct gcgcatcgtg gcggggcatg gtgtctaatc cgggacggat ccggggagat
     2401 tggccatgtc gctttcagct ttgccgcgcg cagcgcgggt cgtgctggtg ctgtgcagcg
     2461 tgttgtcgcc ggtgctggcg aacgcgcagg tgtcccgcca cgtcgaggtc cggctggacg
     2521 ccgccgcgat gcaaccccga gccgccggcc ggctgctggt gttcgccatg cctgccggca
     2581 tggcggaagc cgcggcgaag ggcggcaagg tcgaggaggt ggatgcaagc gcaatggcgc
     2641 ccgaagcggt ggccatcgcc gcgatggagg tgccgtcgct ggccgccggt gcgagcgtgg
     2701 tgctggatgc cgatgcgatc gccttcccgc agccgttctc gcgcctgccc aagggcgact
     2761 actacctgca ggcggtgctc gatgccaacc acgactacaa ctacggcggc cgtggcgagg
     2821 gcgacctcgt cagcgaacca acccgggttc ggctgggcaa gtcgcatcgc atcccgttgc
     2881 tggcgttgac ccgcgtgcag ccggcgcggc cggatccttg ggccgtcgcc gacgacgcgc
     2941 ccgcggacgt gcgcgaggcc atggccgccg cgcgtgcgca gaccgacttg ctcgacttcg
     3001 ccagcccggc cctgagcgcg ttctggggcc gcgacatccg catgcgcggc tgggtcctgc
     3061 tgccgccggg ctatgccgat gccgggaacg cggccacgcg ttatccgagc gcgtatttca
     3121 cccacggctt cggcggcagc ctgccgggcc tggtccggac cgtggcctcg gtggcggcgg
     3181 cgatgcgcga gggcagcatg ccgccgatga tctgggtgtt cctggacgaa tccagcgcca
     3241 ccggcaccca cgagttcgcc gattcggtca acaacggccc ctggggccat gcgctgacct
     3301 ccgaactgat cccgtcgctg gaggcgcgct accggatgga cggcaaggcc tcggggcgcc
     3361 tgctcaacgg ccattcctca ggcggctggg cgacgctgtg gctgcaggtg acctatccga
     3421 agctgttcgg cggcacctgg tcgactgcgc ccgattccag cgacttccat gacttcaccg
     3481 gccccgacct gtacgcgccg gatgccaatg tctatcgccg gccggatggc agtggatggc
     3541 cgctggtccg cgtcgatggc aaggtcgtct ccacgttcga gcagtacgcg cgccaggagt
     3601 cggtgaaggg cgaggtgggt gggcaactgg catcgttcga ttgggtgttc tcgccgcgcg
     3661 gcgcggatgg ccggccgatg ccgatgttcg atcgcgctac cggcaaggtg gatcctgccg
     3721 tggtggccta ctggcgcgag cattacgaca tcgcccacag gctgcaggcg ctatggccgg
     3781 cgttgaagcc cgacctggac ggcaagatcc gcgtcatcgt cggcgatgcc gataccttct
     3841 acctggatgg cgccgcgcgc cggctcaagg ccgtgctgga tggcctgggc gcgaaggcgg
     3901 atttccgttt cctgcccggc aagacccatt tcgacctgta cgtggaaggc gatgaccgcc
     3961 gcgcgctgct gaagaagatc gcgtgggaga tgcatgccgt cgcgcggccc ggcagcgctg
     4021 cggcaccggc cgccattccc ggcgcgctgt gagcgcggat cagctcgcgg cggcgggttc
     4081 gacgcgcgcc ggccgatacg gcggcgtggc catcaccatg tccgccagcg aataggcgag
     4141 ttcgcgttcg gccagcaccg cggcatcggc gccgtgttcg agcaggtgct tcacttccgc
     4201 ctcgctgtgg gcgcgcgcga gcacgctgat cgcgggattc atcgccttga agcgggcgat
     4261 gatctcgccg gcttccagcg cctgcgggat cgccaggatc gccagttgcg cggtatccgg
     4321 gcgggtcgcc ttgaggatcg cttccgaggc ggcgttgccg cgcaccgtga ccaggccgcg
     4381 gttgcgcgcc tgttccaccc tgtccgggtc gctctccacc agcaccagcg gcacgccgcg
     4441 gtcctgcagc aggcgcgaca gctcgcgtcc cacccgtccg tagcccacca cgatggcgtg
     4501 gccgcccatg tcctcgggca cttcggaatt gtcggccagc acctgggtga ccgcctcgcc
     4561 cggctgctgc cgcttctgcc agcggtccag ccaggcgaac agcagcgggt tggcgatgat
     4621 cgacagcagc gcgccggcca ggatcaggtc gtgcccttgc tgcggcagca ccttctggct
     4681 gaggccaagg ccggcgagga tgaaggagaa ctcgccgatc tgcgacaggc tggcggcgat
     4741 ggtcagcgcg gtcagctgcg gcagcctgaa caggcgcacc acggtgaacc cgatcagcgc
     4801 gttgccgaac acgatgatgg ccacggtcgc gaccacgagc agcgggtggt cgaccaggat
     4861 cgacgggttg aacagcatgc ccaccgagac gaagaacagc accgcgaatg catcgcgcat
     4921 cggcaacgaa tcggccgccg ccttgtggct gagttcggac tcgttgagca gcatgccggc
     4981 gaagaacgcg cccagcgcga agctgacatc gaacagcgcc gccgcgccga acgccacgcc
     5041 aagcgcgatc gccagcacgc acagggtgaa aagttcgcgc gagccgagcc cggccacgtg
     5101 ttccaggacc cgcgggatca cccgccgacc gaccaccagc atgaaggcca cgaacgcggc
     5161 caccttcagc agggtccatc cgagcgcgcc gaggatgccg gccgtgccca ctttctcgcc
     5221 agccccgagc gccccggcga tggcgggcag caggaccagc gcgagcacca tcgccaggtc
     5281 ctcgacgatg agccagccga tcgcgatgcg cccgcgccgg gtatcgagca gccggcgatc
     5341 ttccatcgcc cgcatcacca ccacggtgct ggccaccgac agggatagcc cgaacacgat
     5401 gctgcccatc gtctcccagc ccagcatcag gccgaaggcg gtgccgatgg aggtgacgaa
     5461 ggcaatctgc agcagcgcgc cgggcacggc gatgttcttc acgtccagca ggtcgcgtag
     5521 cgagaaatgc aggcccacgc cgaacatcag caggatcacg ccgacctcgg ccagctgcgg
     5581 ggccagggtc tggtcggcga cgaagcccgg ggtgaacggg ccgacgatca cgcccgccag
     5641 caggtagccg accagcggtg acagcttcag ccgctgggcc agcatgccca gcgcgaaggc
     5701 catcacgaag ccgacgcaga gcagggcgat cagcgaggtg tggtgcggca tgcggcgcct
     5761 cggcggggat cggcccctat cttcgcatcc cggcagcggc gcgcatgagc ggcggttcag
     5821 gcaccgcggc ccgatacaat ggtcggatga tctctttccg caatttcgcc ctgcgccggg
     5881 gcgaacgcct tctcctgtcc gatgtcgacc tgaccctgca tgccgggtgg cgggtcggcg
     5941 tgatcggccg caatggcgcc ggcaagtcgt ccctgttcgc cgcgctgatg cgcgaggtcg
     6001 aggccgacaa gggtgacctc gatctcccga cgaaggcgcg catcgcctcg gtggcgcagg
     6061 aaaccccgca cctgcctgac ccggccatcg acttcgtgct gtccggcgac gaagccgtgc
     6121 acgccgccat ccagatggag gccaacgcct tcgccaacga ggactgggaa gcggttgcgg
     6181 aggcccacca tcggctggag gaagtgaacg gttacgacgg ttccgcgcgt gccggccggc
     6241 tgctgcacgg tctcgggttc gccgcctctg cgcacgaaac cccggtctcg gacttctccg
     6301 gcggctggcg ggtgcgcctg aacgtggcgc gcgcgctgat gacgcccagc gacctgctgc
     6361 tgctcgacga gccgaccaac cacctcgacc tcgatgccgt ggtgtggatg gaggaatggc
     6421 tgaagcgcta cgacggcacc ctgctggtga tctcgcacga ccgcgagttc ctcgacaacg
     6481 tcaccaccca caccctgcac ctgcacgagg gcaaggccaa gctgtacgtc ggcaactaca
     6541 ccgatttcga acgccagcgc gccgagcacc tgcgccagca gcagatcgcc caccagaagg
     6601 aacaggcgga gcgcgcgcac ctgcagtcgt tcatcgaccg cttcaaggcc aaggcgagca
     6661 aggccaagca ggcgcagagc cggatgaagc ggctggccaa gctggccggc accgaagcgg
     6721 tgcgcgccga gcgttcgctg cacatcaccc tgccggaacc gttgaagctg ccgaattcgc
     6781 tactgtcgct gcacgatgtc gaagccggct atggcgcgga tgcagtgatc ctgcacaacg
     6841 tcaacttcgg cctggaggcg ggcgaccgca tcggcctgct cggcccgaac ggtgccggca
     6901 agtcgaccct ggtgaagacc ctggtcggcg agctcgcgcc gatgaccggc g
//
LOCUS       DAOOCX010000114         6873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_10342_length_6873_cov_7.439132, whole genome shotgun sequence.
ACCESSION   DAOOCX010000114 DAOOCX010000000
VERSION     DAOOCX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10342_length_6873_cov_7.439132"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..2970)
                     /locus_tag="PK399_09785"
     CDS             complement(22..2970)
                     /locus_tag="PK399_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPW13448.1"
                     /translation="MNDAPALILRDRRLARALLPCAIVLALAACGGGGGNVRPSTPTP
                     PSTPGGTVLDFTPTVANDSAIVVNPPAIPTLGAPVTWAAPGINRHLSLTNAGGALGAG
                     LTGQGVTIGFVDSGVNRNHPTLSGRVTSNFVHVSSPPNNTSVDDVVGHGTTVASLAAG
                     KPATGVYSAGGSDTWGGGIAQGAGVASSRIIGDARPDDDGSGEGNEIRAGEGYGEFFQ
                     AINAELAGAGAKIINNSWGGLYWNDPALSIELANAWKDFVVTRGGIVVFANGNSGDDP
                     RFVGNPSDNAALPSIANDPVLEKGWLTVGALDPLNPTQLTGYSQQCGIAMNYCLVAPG
                     NVVFIDPDATTQANSVLYQGGGTSYAAPLVSGAAAVVWSAFPYFSNDQVRQTVLAASR
                     DLGAPGVDSVFGWGLLDVTKAAMGPSNFAWGDFSVAFSGNSIWRNPIIGSGGLIKGGS
                     GTLTLAEAGNFTGATRVDAGGLDVRKGLRSNLGIASGATVWASGVFGGNVSNSGRFYS
                     GASSPASISGNFIQSSTGNLGVWLGSSLRVTGTATLDGQMSILGVRSGYTTTAKETLL
                     NATGGVSGTFSALRAASNVFLDASLAYDPNNVFLNINRINVANAVAGMGLSTITQVSA
                     ARVESAMSAIDGQLAGTLPVGIGAAFIDAAGALQQASSIANADVSLRSLSGQLHGASA
                     AMTFDAIDAGRRALDGRLDALSLAPHATGGWYRDLSSGGTLAQAGFDSVGIDAAGEMI
                     GNDWRVGRHGVVGIALNRLEQSGWLSDFGDRSRGRQRELQVYAATWLGDWHAQAQLAS
                     GSFQRQMQRNLLLGAMRDAVATRLSGDYVGASAELGRRFDAGGIALTPYLGTHVVQIR
                     NDGFDEGGASGFGLRANAWDSRRWQGFAGLRATHGWRVGDIDLRADARAEWQRTLAAS
                     GAAFDASYSGLEQWAPLQGIGLADRGNLIGTGLSASIGSRATFRFDLSRRSSPVGDNT
                     LASLQASWRF"
     gene            3164..4339
                     /locus_tag="PK399_09790"
     CDS             3164..4339
                     /locus_tag="PK399_09790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005911622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPW13449.1"
                     /translation="MSDVVIVGAKRTAIGSFLGQFTGVPSPTLGTAAIRGALAQAGVA
                     ADQVDEVIMGCVLPAGLGQAPARQASRAAGIPDAAGCTTINKVCGSGMKAVMLAHDII
                     KAGSASTVVAGGMESMTNAPHLLNGSRTGIRFGAGELVDHMAWDGLTNPDDGKAMGVF
                     GDAACAKYGYDRAAVDAYAAESVRRAQAAQASGAFDGEIAAVTVKTRKGEVVVDKDEE
                     PGKIDPSKIPDLRAAFGKDGVLTAASSSKISDGAAALVVMSSEAAAAAGATPLARIVA
                     HATHAQAPEWFTTAPVTALRNVLAKAGWSVADVDLFEVNEAFACVAMAPMTELGIAHD
                     KLNVNGGALALGHPIGASGARLLVTLLNALQARGGKRGVASLCIGGGEAVALAVELV"
     gene            4478..4840
                     /locus_tag="PK399_09795"
     CDS             4478..4840
                     /locus_tag="PK399_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13450.1"
                     /translation="MSINKILLAMALGLALTACGNQEQAADAAADATAAAGEAAAAAD
                     AAAATGDAASADAAQAAADTAATAADAAAASADATAAAAGTAAAGDAADAAMDAADSA
                     EQAAAAAQNAAASAEEKK"
     gene            5049..5429
                     /locus_tag="PK399_09800"
     CDS             5049..5429
                     /locus_tag="PK399_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13451.1"
                     /translation="MNTKLYVLLAAGVLALSACKNETATAQDAAADAAAATDQAAAAA
                     GDAAAAAGDAAAAGVDAAAAATGEAVGETAAAVEGAADTAAAAANEATAAAADATADA
                     ANAVADKAAATADKAEAAAAEAKK"
     gene            5559..>6873
                     /locus_tag="PK399_09805"
     CDS             5559..>6873
                     /locus_tag="PK399_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPW13452.1"
                     /translation="MPALTSQLDPRSQDFLDNASWHRDLVAELDRRLARAAGGGGEQA
                     RARHTERGKLLPRERIDALLDPGSPFLEIAPLAAEGMYDDAAPAAGMVCGIGRVMEQD
                     VVIVANDATVKGGTYFPMTIKKHLRAQEIARENRLPCIYLVDSGGAFLPLQDEVFPDK
                     EHFGRIFYNQARMSAENIPQIAVVMGSCTAGGAYVPAMCDESIIVKEQGTIFLGGPPL
                     VKAATGEVVDAETLGGADVHTSMSGVADHFAEDDRHALEIARAIVGTLNRRKVLPVAA
                     VASRDPLFAADELYGIVPKDARRPFDIREVIARIVDGSEFQEFKARYGKTLVTGFAHL
                     HGYPVGIVANNGILFAESALKGAHFIELCNQRGIPLVFLQNITGFMVGKKYEQAGIAK
                     DGAKMVTAVACSHVPKFTVVIGGSFGAGNYAMCGRAYGARFLWMWP"
BASE COUNT         1089 a         2440 c         2277 g         1067 t
ORIGIN      
        1 ctgaccccac ttttctcctg ctcagaaccg ccaggacgcc tgcagcgagg ccagtgtgtt
       61 gtcgcctacc gggctcgagc gacggctcag gtcgaagcgg aacgtcgccc ggctaccgat
      121 actcgccgac aggccggtgc caatgaggtt gccgcgatcg gccagcccga tgccctgcaa
      181 cggcgcccac tgttccagcc cgctgtagct ggcatcgaag gcggcgccgc tcgccgccag
      241 ggtgcgctgc cattcggcgc gcgcgtcggc acgcaggtcg atgtcgccca ctcgccaacc
      301 atgcgtcgcg cgcaacccgg cgaagccctg ccagcgacgg ctgtcccacg cgttcgcgcg
      361 caggccgaaa ccgctcgcgc cgccttcgtc gaagccgtcg ttgcggatct gcacgacgtg
      421 cgtgcccagg tatggcgtga gcgcgatgcc gccggcatcg aagcgtcgac caagctcggc
      481 ggacgccccg acgtaatcgc ccgacaatcg cgtcgccacc gcgtcccgca tcgcgcccag
      541 cagcaggttg cgctgcatct gccgctggaa ggaaccggat gccagttgtg cctgcgcatg
      601 ccagtcgcca agccatgtcg ctgcatagac ctgcaattcg cgctggcggc cgcggctgcg
      661 atcgccgaag tcgcttagcc agccggactg ctccagccgg ttcagcgcga tgccgacgac
      721 gccatggcga ccgacgcgcc agtcgttgcc gatcatctcg ccggccgcat cgatgccgac
      781 gctgtcgaag ccggcctgcg ccaacgtgcc gccgctggac agatcccgat accagccgcc
      841 cgtcgcgtgc ggggcaagcg atagcgcatc cagtcgaccg tccagtgcgc ggcgcccagc
      901 gtcgatcgca tcgaaggtca tcgccgccga tgcaccatgc aactggcccg acaggctgcg
      961 cagcgacaca tccgcattgg cgatgctgga agcctgctgc aacgcgcccg cggcatcgat
     1021 gaacgccgcg ccgatgccca ccggcaaggt gccggccagt tggccgtcga tcgcggacat
     1081 cgccgattcc acccgtgccg ccgacacctg ggtgatcgtc gacaggccca tgcccgcgac
     1141 cgcgttggcc acgttgatgc ggttgatgtt gaggaagacg ttgttggggt cgtaggccag
     1201 gctggcgtcc aggaacacgt tcgacgcggc cctgagcgcg gagaacgtgc cgctgacgcc
     1261 gccggtcgca ttgagcaggg tttccttcgc ggtcgtggtg tagccgctcc tgaccccgag
     1321 gatcgacatc tgcccatcga gggtggcggt gccggtgacc cgcaggctgc tgcccagcca
     1381 cacgccgagg ttcccggtcg aggactggat gaaattgccg ctgatgctgg ccgggctgct
     1441 ggcgccgctg tagaagcgac cgctgttgct cacgttcccg ccgaagacgc cgctggccca
     1501 gaccgtcgcc ccactggcaa tgccgaggtt ggagcgcaat cccttgcgca cgtccagccc
     1561 gccggcatcc acgcgcgtcg cacccgtgaa gttgccggcc tccgccaggg tgagggtccc
     1621 gctgccaccc ttgatgaggc cgccactgcc gatgatgggg ttgcgccaga tggagttgcc
     1681 gctgaacgcc acgctgaaat cgccccacgc gaaattgctc ggccccatcg ccgccttggt
     1741 gacatcgagc aagccccaac cgaacacact gtccacgccg ggcgcgccca ggtccctgga
     1801 ggccgccagc acggtttgcc gcacctggtc gttgctgaaa tacgggaacg ccgaccacac
     1861 caccgcggct gcgccggata ccagcggcgc ggcgtacgac gtaccgccgc cctggtaaag
     1921 gacgctgttc gcctgggtcg tcgcatcggg atcgatgaac accacgttgc cgggcgcgac
     1981 caggcaatag ttcatggcga tgccgcactg ctgcgagtag ccggtcagct gggtcgggtt
     2041 gagcggatcc agcgcgccga ccgtcaacca gcctttttcg aggacgggat cgttggcgat
     2101 gctcggcagt gccgcattgt cggagggatt gccgacgaag cgcggatcgt cgccgctgtt
     2161 gccgttggcg aacaccacga ttccgccgcg ggtcaccacg aaatccttcc aggcattcgc
     2221 aagctcgatg ctcagcgccg gatcgttcca gtacagccca ccccaggagt tgttgatgat
     2281 cttcgcgcct gccccggcaa gttccgcgtt gatcgcctgg aagaactcgc cgtagccctc
     2341 gcccgcacgg atttcgttgc cttcgccgga gccatcatcg tcgggcctcg catcgccgat
     2401 gatgcgcgat gacgccacgc cggcgccctg cgcgatcccg ccgccccagg tgtcgctgcc
     2461 acccgcgctg tagacgccgg ttgcgggctt gcccgccgcc agcgacgcaa cggtggtgcc
     2521 gtgcccgacc acgtcatcca cgctcgtgtt gttgggcggc gacgacacat ggacgaagtt
     2581 gctggtcacg cggccactga gggtgggatg gttgcggttc acgccggaat cgacgaaccc
     2641 gatcgtcacg ccttgaccgg tgaggcctgc gcccagtgcg cctcccgcat tggtcaggct
     2701 caggtgtcga ttgatgccgg gtgcggccca ggtgacgggc gcgccaaggg tcggaatcgc
     2761 tggcgggttc accacgatcg cactgtcgtt cgccaccgtc ggggtgaagt ccaggacagt
     2821 gccgcctggc gtgctgggtg gtgttggcgt gctcggccgc acattgccac cgccaccgcc
     2881 acaggccgcc agggccagca cgatcgcgca ggggaggagc gcgcgcgcca ggcgacggtc
     2941 gcggaggatg agtgcgggtg cgtcattcat ggtcattccc caggtgcgcc gccggcccct
     3001 cgccgacgcc atgcgccctg cgggtgttgt atgccgcacc cgccgtttcc gcaacgccgg
     3061 cagcctggat cggccacgga tgcggcggcg cagcatgccc ctcggattcg cgccataatc
     3121 ggccattcga cccaagcggc ccatccggcc cggagccccc accatgagcg acgtcgtcat
     3181 cgtcggtgcc aagcgcaccg ccatcggttc cttcctcggc cagttcaccg gcgttccctc
     3241 gcccaccctg ggcacggctg cgatccgggg cgcgctggcg caggccggcg tggccgccga
     3301 ccaggtcgat gaagtgatca tgggctgcgt gctgcctgcc ggccttggcc aggccccggc
     3361 acggcaggcc tcgcgcgccg ccggcatccc cgatgccgcc ggctgcacca caatcaacaa
     3421 ggtctgcggc tcgggcatga aggcggtgat gctggcgcac gacatcatca aggccggttc
     3481 ggccagcacg gtggtcgccg gcggcatgga gtcgatgacc aatgcgcccc acctgctcaa
     3541 cggctcgcgc accggcatcc gctttggcgc cggcgagctg gtcgaccaca tggcctggga
     3601 cggcctgacc aatcccgatg acggcaaggc gatgggcgtg ttcggcgatg ccgcctgcgc
     3661 caagtacggc tacgaccgcg ccgcggtcga tgcctatgcc gcagagagcg tcaggcgcgc
     3721 ccaggccgcg caagccagcg gggccttcga cggcgagatc gccgcggtca ccgtcaagac
     3781 ccgcaagggc gaggtggtgg tcgacaagga cgaggagccc ggcaagatcg acccatcgaa
     3841 gatccccgac ctgcgtgcgg ccttcggcaa ggatggcgtg ctgacggcgg cgtcctcgtc
     3901 caagatttcg gacggcgccg ccgcgttggt ggtgatgtcg tccgaggctg cggccgccgc
     3961 cggcgcgacg ccgctggcgc gcatcgtcgc ccatgccacg catgcccagg ccccggaatg
     4021 gttcaccacg gcaccggtca ccgcgctgcg gaacgtgctc gccaaggccg gctggtcggt
     4081 ggccgatgtc gacctgttcg aggtcaacga agccttcgcc tgcgtggcga tggcgccgat
     4141 gaccgagctc gggatcgcgc acgacaagct gaatgtgaat ggcggggcgc tggcgctggg
     4201 ccacccgatc ggtgcctcgg gtgcgcggct gctggtgaca ttgctgaatg cgctgcaggc
     4261 ccgcggcggc aagcggggcg tagcgtcact gtgcatcggc ggcggcgaag cggtcgccct
     4321 ggccgtcgag ctggtgtaaa agtcccgtta ggcggtgaac ggccgattca aaaaaaaccg
     4381 gccagccgct tgacacgcga cacgggcttg tcatcatgtt accggcgcgc agtatgcgcg
     4441 ttctgaaaac accctattca tacgacgagg aatacacatg tccatcaaca agatcctgct
     4501 ggcgatggcg ctgggcctgg ccctgaccgc ttgcggcaac caggagcagg ctgctgacgc
     4561 cgctgccgac gccaccgccg ctgccggcga agccgccgct gccgctgacg ccgctgccgc
     4621 taccggcgac gccgcttcgg ctgacgccgc ccaggccgct gccgacaccg ccgccaccgc
     4681 tgctgacgcc gctgccgctt cggccgacgc gaccgccgct gctgccggta ccgccgctgc
     4741 cggcgacgct gccgatgccg ccatggacgc cgctgactcg gccgagcagg ctgctgccgc
     4801 tgctcagaac gccgctgctt cggctgaaga gaagaagtaa tcgactcggc gggctagctc
     4861 gccggttcgt tactgtagaa tcggagaagc cgtcggtacg ccgacggctt tttcgtgccc
     4921 cgaaccatcg gggctgttgt gagtcagtga acgccccgcc ggccacgcat gccggatcgc
     4981 caagcgcact ggcgttgcct gatccgaacc cgttgcattt cagacaaccc ctaatccagg
     5041 agatttccat gaacaccaag ctttacgtgc tcctcgccgc tggcgtcctc gctctgtcgg
     5101 cctgcaagaa tgaaaccgcg accgcgcagg acgccgctgc cgacgctgct gccgcgaccg
     5161 accaggccgc cgctgcggct ggcgacgccg ctgccgctgc cggcgacgcc gccgctgccg
     5221 gcgttgacgc cgctgccgcc gccaccggcg aagccgttgg cgaaaccgcc gctgccgtgg
     5281 aaggcgctgc cgacaccgcc gctgctgccg cgaacgaagc caccgccgcc gctgccgacg
     5341 ccaccgccga cgccgccaat gcggtcgccg acaaggctgc cgcgaccgcc gacaaggccg
     5401 aagccgctgc tgccgaagcc aagaagtaat cttcttcgct ttgctgtccc tggaaggccc
     5461 gcttcggcgg gccttcctct ttctggaacc acgcacgtcg gcggaccggc gcaccgcagc
     5521 ggttatgctt tcgcattgac cgcaccgtcg atgaccgcat gcccgcgctg acctcgcaac
     5581 tcgatccccg ctcgcaggat ttcctcgaca acgcctcctg gcatcgcgac ctcgtcgccg
     5641 aactcgatcg tcggctggcc cgcgccgcgg gcggcggtgg cgagcaggcc cgcgccaggc
     5701 acaccgaacg cggcaagctg ctcccgcgcg aacgcatcga tgccctgctc gatcccggct
     5761 cgccattcct ggaaatcgcc ccgctggcgg ccgaaggcat gtacgacgat gccgcgcccg
     5821 ccgccggcat ggtctgcggc atcggccggg tgatggagca ggacgtcgtg atcgtcgcca
     5881 acgacgccac ggtcaagggc ggcacctatt tcccgatgac catcaagaag catttgcgcg
     5941 cgcaggaaat cgcccgcgag aaccgcctgc cctgcatcta cctggtcgat tccggcggcg
     6001 ccttcctgcc attgcaggac gaggtgttcc cggacaagga acacttcggc cggatcttct
     6061 acaaccaggc gcggatgagc gcggagaaca tcccgcagat cgccgtggtg atgggctcgt
     6121 gcaccgccgg cggtgcctac gtgccggcga tgtgcgacga atcgatcatc gtcaaggaac
     6181 agggcacgat cttcctgggt ggcccaccgc tggtgaaggc ggccaccggc gaagtggtcg
     6241 atgccgaaac cctgggcggt gccgacgtgc acaccagcat gtccggcgtg gccgaccatt
     6301 tcgccgagga cgaccgccac gcgctggaga tcgcacgcgc catcgtcggc acgttgaatc
     6361 gccggaaggt gctgccggtg gcggcggtgg cttcgcgcga ccccttgttc gcagcggacg
     6421 agctgtacgg gatcgtgccg aaggacgcgc gccggccctt cgacatccgc gaggtgatcg
     6481 cgcgcatcgt cgacggcagc gagttccagg agttcaaggc gcgctacggc aagaccctgg
     6541 tgaccggctt cgcccacctg cacggctatc cggtcggcat cgtcgccaac aacggcatcc
     6601 tgttcgcgga atcggcgctg aagggcgcgc acttcatcga gctgtgcaac cagcgcggca
     6661 tcccgctggt gttcctgcag aacatcaccg gcttcatggt cggcaagaag tacgaacagg
     6721 ccggcatcgc caaggatggc gcgaagatgg tgaccgcggt ggcgtgcagc cacgtgccga
     6781 agttcaccgt ggtgatcggt ggcagcttcg gtgccgggaa ctacgccatg tgcggccgcg
     6841 cctacggcgc gcgcttcctg tggatgtggc cga
//
LOCUS       DAOOCX010000115         6863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_10359_length_6863_cov_8.570652, whole genome shotgun sequence.
ACCESSION   DAOOCX010000115 DAOOCX010000000
VERSION     DAOOCX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10359_length_6863_cov_8.570652"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PK399_09810"
     CDS             <1..696
                     /locus_tag="PK399_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPW13453.1"
                     /translation="LVVLPHGGPYLVDDTWGFDEEVQFLASRGYAVLQVNYRGSSGRG
                     ETYEQAGWKGWGTKIQDDITDAVKWTIDQKMTDPNRICMFGASFGGYSALVQPVLNPG
                     MYKCAIGYVGVYDLPLMRKTDNRNGQSDATERFFDRTLGTDLAALAKVSPAQRAGDVK
                     VPVFLVHGKDDKTADFNQFKVMEAALKAAGNPAQVMLADGEGHGFVKPENRAELYRRM
                     EAFLAKYIGPGAK"
     gene            complement(747..1547)
                     /gene="hutG"
                     /locus_tag="PK399_09815"
     CDS             complement(747..1547)
                     /gene="hutG"
                     /locus_tag="PK399_09815"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HPW13454.1"
                     /translation="MSDLPDVDVFTLHRGSAPLLLSLPHDGSLIPESLQARMTPQARV
                     APDTDWHVSRLYDFARDLGASILVPTYSRYVIDLNRGETDTSLYPGQNTTGLVPLVRF
                     TGEPIYLPGREPDADEVASRIDTCWRPYHVALRGELDRLRAMHGRVALWEGHSIRGSG
                     LPFLFEGRLPDLNLGTANGASCSPALQARIETVLAGQGRYDWVANGRFKGGHITRHYG
                     APADGIDAVQLELSQRTYMDEDSFDYDAARAARLQPLLRALLQATLAP"
     gene            complement(1555..2814)
                     /locus_tag="PK399_09820"
     CDS             complement(1555..2814)
                     /locus_tag="PK399_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW13455.1"
                     /translation="MNDNPIDVLVVGAGVSGLATALALLNDGRAVTVIDAGRIGGGAS
                     HGNCGTLTPSHATPLAAPGTIAKAMRWMFRKDAPLYVPPRFDPLLWRWMAGFALRCNL
                     RDWEASARAKYTMLADSRLRVQRWIEDYGLDCDFVESGEDYVFRDPRTMASEMHELPL
                     LRDLGIDVEVIDGAAYEAQEPALKPGVAGAIRFSGDAALRPDRYVAELARVVRERGGQ
                     VVEDCALHALEANNDGVVAVTAQGTLRARDAVIATGAWSPRIAREIGLPWLRKAIQPG
                     KGYSMTYSRPALVPKRPMILHGPSVCVSSWPGGFRLGSTMEFSGYDDSLNEARLGALE
                     RGARQFLHEPVGPELREKWFGWRPMSRDDIPLLGRAPGHRHLWLAVGHGMMGVGMSAG
                     TGQLMADLITGRTPALDPAPFDPARFA"
     gene            complement(2891..4342)
                     /locus_tag="PK399_09825"
     CDS             complement(2891..4342)
                     /locus_tag="PK399_09825"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HPW13456.1"
                     /translation="MSQEFQPVAIRDRIEVLDVVRGFALLGIALMNVEFFNRPIADLD
                     AGMPAGITGIDYWAGWFVHVFVRGKFWTMFSLLFGMGFAVMLTRAEQAGRGFFVPYLR
                     RTLALALFGALHFIFLWTGDILFSYAAGAVLLMLVFYSKPRILLWLGVSLLALAAAFG
                     AAGSFGHELPWQPMLGFGIPLLLFGVVAYSLRRWPLSGLRAAGLALYLVPALAMTIGG
                     AVMVGQPPQAERDRLQQGQAKTPEQKKALAEQLEKRTEQRAEHARDVADETRLMSTGR
                     YADATAWRAQSFAKHLGQTVGFAVIVLGVFLLGAWFIRSGVMSDPASHLRLFRQMAWI
                     GIPLGLGLGLAGAGISMTHVRGQNDAAFQLASGLAFIGNLPACLGYLGAVVLLFHGRW
                     RRWLAHLAPVGRMALSNYILQSLLGTWFFYGYGLGHWGLPRSQQVVYVLVVFALQVLL
                     SRWWLSRFRFGPLEWAWRWITYGKRPPMRASIR"
     gene            complement(4484..4966)
                     /locus_tag="PK399_09830"
     CDS             complement(4484..4966)
                     /locus_tag="PK399_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW13457.1"
                     /translation="MKIEKDRVVRFHYAVAEAGQESVENSKDGGQPLAILFGHGNIIP
                     GLEKAMDGREAGDSFKANVPASEAYGERRDGLTQRVPKKHFGAQKLEPGMQVVLNTNF
                     GPRAVTIQKVGMSVVDVDLNHPMAGKDLEFDIEIVEVRDASAEELAHGHVHGDGGHQH
                     "
     gene            5036..5611
                     /locus_tag="PK399_09835"
     CDS             5036..5611
                     /locus_tag="PK399_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005912976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPW13458.1"
                     /translation="MGPADCQQRPGAHDTPTCWRNARLALSTALTAILLLAGCGGNEN
                     VRPSASRQAPARDWPVVQPADPAKANAVLMRAISLVGTPYRYGGNTPEGGFDCSGLVN
                     YVFRDMLDLRLPRTSRELAATQGPRIATDRLAGGDLVFFGSDGLVSHVGIYVGEGRFV
                     HAPNSGGTVRLDRLDGSWWRDHYTGARRLLN"
     gene            5849..6430
                     /locus_tag="PK399_09840"
     CDS             5849..6430
                     /locus_tag="PK399_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPW13459.1"
                     /translation="MALAACAAPAMAITPTSPPADAAPQVTVATQAPLDAVAPVDLAS
                     PDNAPLLAQIAALQGAQNAPSTSSRIKSVLQRAFSLLGTPYRWGGTSPEGGFDCSGLV
                     GYVFRTIGIDLPRVSRSMAQEGTAVTDRTALAEGDLVFFGKRGRVDHVGIYIGEGKFL
                     HAPRTGRDVTVSSLDSGYWSQKYLQARRLATGA"
     gene            complement(6483..>6863)
                     /locus_tag="PK399_09845"
     CDS             complement(6483..>6863)
                     /locus_tag="PK399_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPW13460.1"
                     /translation="AAAVGWSGRYSVTVAVGGIRFVAPIRISDLVTVETTLVHTGTSS
                     MHFAVDVRARDPMQDAGERLCTHCVIVFVALDGVEGTPVAVPAWTPDSEEDHRLAEYA
                     MQVMALSKDIEQTVARYREAGRKP"
BASE COUNT         1126 a         2452 c         2252 g         1033 t
ORIGIN      
        1 ctggtggtgc tgccgcatgg cggcccctac ctggtggacg atacgtgggg cttcgacgag
       61 gaagtgcaat tcctggccag ccgcggctac gcggtgctcc aggtgaacta ccgcggctcg
      121 agcggccgtg gcgagaccta cgagcaggcg ggctggaagg gctggggcac caagatccag
      181 gacgacatca ccgatgcggt gaagtggacc atcgaccaga agatgaccga cccgaaccgc
      241 atctgcatgt tcggtgccag cttcggcggc tactcggcgc tggtgcagcc cgtgctcaac
      301 cccggcatgt acaagtgcgc gatcggttat gtcggggtct acgacctgcc gttgatgcgc
      361 aagaccgaca accgcaacgg ccagagcgac gccaccgaac gcttcttcga ccgcaccctc
      421 ggcaccgacc tcgccgcgct ggccaaggtc tcgccggcgc aacgtgcggg cgacgtcaag
      481 gtgccggtct tcctggtgca cggcaaggac gacaagaccg ccgacttcaa ccagttcaag
      541 gtcatggagg ccgcgctgaa ggctgccggc aatcccgccc aggtgatgct tgcggacggc
      601 gagggacatg gcttcgtgaa gcctgaaaac cgcgcggaac tctaccgccg catggaagcg
      661 ttcctggcca agtacatcgg ccccggcgcg aagtaagcaa tcgacccgag gttccccgaa
      721 gggcggcgca agccgccctt cgtcgttcag ggcgccagcg tcgcctgcag caatgcacgc
      781 aacagtggct gcagccgggc agccctggcc gcgtcgtaat cgaagctgtc ctcgtccatg
      841 taggtgcgct ggctcagttc gagttgcacc gcgtcgatgc catcggccgg cgcgccgtag
      901 tggcgagtga tgtgtccacc cttgaagcga ccgttggcga cccagtcgta cctgccctgc
      961 cccgccagca cggtttcgat gcgtgcctgc agcgcgggcg aacaactggc gccattggcg
     1021 gtgcccaggt tgaggtcggg caggcggccc tcgaacagga atggcaggcc gctgccgcgg
     1081 atcgaatggc cttcccacaa cgcaacgcgc ccgtgcatcg cgcgcaggcg atccagctcg
     1141 ccgcgcaagg cgacgtggta cggccgccag caggtgtcga tgcgcgaggc gacctcatct
     1201 gcatccggct cccggcccgg caggtagatc ggctcgccgg tgaagcggac cagcggcacc
     1261 agtccggtgg tgttctggcc gggatacagg ctggtgtcgg tctcgccgcg gttgaggtcg
     1321 atcacgtagc gcgaatacgt cggcaccagg atcgatgcgc ccaagtcgcg cgcgaaatcg
     1381 tagagccgcg acacgtgcca gtcggtatcc ggcgccacgc gtgcctgcgg ggtcatccgc
     1441 gcctgcaggg attccgggat caggctgccg tcatgcggca ggctgagcag cagcggcgcg
     1501 ctgccgcgat gcagggtgaa cacgtcgacg tcgggcaggt cgctcatcgc tgcgtcacgc
     1561 gaagcgcgcg ggatcgaacg gcgccgggtc cagcgcgggc gttcggccgg tgatcaggtc
     1621 ggccatcagc tggccggtgc cggcgctcat gcccacgccc atcatcccgt ggcccaccgc
     1681 cagccacagg tggcggtggc ccggcgcgcg gccgagtagc gggatgtcgt cgcggctcat
     1741 cggccgccag ccgaaccact tctcgcgcaa ctcgggaccc accggttcat gcaggaactg
     1801 gcgcgcaccg cgttccaggg cgcccagccg ggcctcgttg aggctgtcgt cgtagcccga
     1861 gaactccatg gtgctgccca ggcggaaccc gcccggccag gacgacacgc agaccgacgg
     1921 cccgtgcagg atcatcggcc gcttcggcac caacgcgggt cgactgtagg tcatcgaata
     1981 acccttgccg ggctggatcg ccttgcgcag ccacggcagg ccgatttcgc gggcgatccg
     2041 cggcgaccat gcgccggtgg cgatcactgc gtcgcgggcg cgcagcgtgc cctgcgcggt
     2101 caccgcgacc acgccgtcgt tgtttgcctc cagcgcatgc agggcgcagt cctcgacgac
     2161 ctgaccgccg cgctcgcgga cgacgcgcgc gagttccgcg acgtagcggt ccggccgcaa
     2221 cgcggcgtcg ccgctgaaac ggatcgcacc ggccacgccg ggcttgagcg caggttcctg
     2281 ggcctcatac gcagcgccgt cgatgacttc gacgtcgatg ccgaggtcgc gcagcaacgg
     2341 gagctcgtgc atctcgctcg ccatcgtgcg cgggtcgcgg aacacgtagt cctcgccgct
     2401 ctcgacgaag tcgcagtcca ggccgtagtc ctcgatccag cgctgcacgc gcagccgcga
     2461 atcggcgagc atcgtgtact tcgcgcgcgc actggcttcc cagtcgcgca agttgcagcg
     2521 cagggcgaac ccggccatcc agcgccacag cagcggatcg aaacgcggcg gcacgtacag
     2581 cggcgcgtcc ttccggaaca tccagcgcat cgccttggcg atggtgcccg gcgcggccag
     2641 cggtgttgca tggctcgggg tcagcgtgcc gcaattgccg tgcgaggcgc cgccaccgat
     2701 gcggccggca tcgatcaccg tgaccgcgcg cccatcgttc aacagcgcca gcgcggtggc
     2761 caggccggag accccggcac cgaccaccag cacatcgatc gggttgtcgt tcaatcgcga
     2821 tgtcacgcgg tcatcccggt ggaggcaagg ccaccattat ccggggaaga tccacctccg
     2881 ccaccgttcc tcagcggatg gacgcccgca tcggcggtcg cttgccgtag gtgatccaac
     2941 gccacgccca ttccagcgga ccgaagcgga acctcgacag ccaccagcga ctgagcagca
     3001 cctgcagcgc gaacacgacc agcacgtaca ccacctgctg gctgcgcggc aggccccaat
     3061 gccccagtcc gtagccgtag aagaaccacg tgcccagcag ggattgcagg atgtagttgc
     3121 tcagcgccat ccgcccgacc ggcgccaggt gcgcaagcca gcggcgccag cggccgtgga
     3181 acagcagcac caccgcaccg aggtaaccca ggcatgccgg caggttgccg atgaaggcca
     3241 gcccggacgc cagttggaac gcagcgtcgt tctgcccgcg cacatgggtc atggagatac
     3301 ccgcaccggc caggcccagg ccgagcccca acgggatgcc gatccatgcc atctgccgga
     3361 acaggcgaag gtgcgatgcg ggatccgaca tcaccccgga ccggatgaac cacgcaccca
     3421 gcaggaagac gcccagcacg atcaccgcga accccacggt ctggcccagg tgcttggcga
     3481 aggattgcgc gcgccaagcg gtcgcgtccg cgtagcggcc cgtgctcatc aggcgggttt
     3541 catcggcaac gtcccgcgca tgctccgcgc gctgttccgt gcgcttttcc agctgctccg
     3601 cgagcgcctt cttctgctcc ggcgtcttcg cctgcccttg ctgcaggcga tcgcgttcgg
     3661 cttggggcgg ctggccgacc atcaccgcac cgccgatggt catcgccagc gccgggacca
     3721 ggtacagcgc cagccccgcc gcgcgcagcc ccgacagcgg ccaacgacgc agggagtacg
     3781 ccaccacccc gaacaacagc agcgggatgc cgaagccgag catcggttgc caggggagtt
     3841 catgaccgaa cgaacccgcc gccccgaagg cggccgccag cgccagcagc gacacgccca
     3901 gccacagcag gatccgtggc ttcgaataga acaccagcat cagcagcacc gcgcctgccg
     3961 catagctgaa gaggatgtcg ccggtccaca ggaagatgaa atgcagcgcg ccgaacaggg
     4021 ccagggccag ggtgcgccgc aggtagggca cgaagaaccc gcgacccgcc tgctcggcgc
     4081 gggtgagcat caccgcgaag cccatcccga acagcagcga gaacatcgtc cagaacttgc
     4141 cgcggacgaa cacatggacg aaccagccgg cccagtagtc gatcccggtg atgcccgccg
     4201 gcatgccggc atccaggtcc gcgatcggcc ggttgaagaa ctcgacgttc atcagcgcga
     4261 tgccgagcag ggcgaacccg cggacgacgt ccagcacctc gatgcggtcg cggatcgcga
     4321 ccggctggaa ttcctggctc attcgacatt cccccatgga ggcaccgatt agcgcacgcg
     4381 gcaggggcgt acacgcgggc cggaagtcat tgcgcctttc gtcgccgcca acgaaaacgg
     4441 cccgcgtcgc gggccgtttc gatgcgcagg cctgcaggca ggctcagtgc tggtggccac
     4501 cgtcgccgtg cacgtggcca tgcgccagtt cttccgccga ggcgtcgcga acctcgacga
     4561 tctcgatgtc gaactccagg tccttgcccg ccatcgggtg attgaggtcc acgtcgacca
     4621 cgctcatccc gaccttctgg atggtgaccg cacgcgggcc gaagttggtg ttcaggacca
     4681 cttgcatgcc cggctcgagc ttctgcgcac cgaaatgctt cttcggcacg cgctgggtca
     4741 ggccgtcgcg gcgctcgccg taggcctcgc tggccggcac attggccttg aagctgtcgc
     4801 cggcctcgcg accgtccatc gccttttcca ggccggggat gatgttgccg tggccgaaca
     4861 ggatcgccag cggctggccg ccgtccttgg aattttccac ggactcctgg ccggcttcgg
     4921 caacggcata gtggaaacgg acgacgcggt ctttctcgat cttcatgggc gctctcggtc
     4981 ttggtgcagt tgcgcgcttg ccgccgaacg gcgatgcggg aacactgcgc ggatgatggg
     5041 accagccgac tgccagcagc gaccgggcgc gcatgatacg cccacttgtt ggcgcaatgc
     5101 ccggctggcg ctatcgaccg ccctgactgc gatcctgctg ctggcgggct gcggcggcaa
     5161 cgaaaacgtc cgcccgagcg caagccggca agcgcccgcg cgcgattggc ccgtcgtcca
     5221 gcccgctgat ccggccaagg ccaatgccgt gctgatgcgc gcgatcagcc tggtcggcac
     5281 gccgtaccgg tatggcggca acacgccgga gggtggcttc gactgcagcg gcctggtcaa
     5341 ttatgtcttt cgcgacatgc tggacctgcg cctgccgcgg acgtcgcgtg agctcgccgc
     5401 cacgcagggg ccgcggatcg ccaccgaccg gctggccggc ggcgacctgg tgtttttcgg
     5461 cagcgatggc ctggtcagtc acgtcggcat ctatgtcggc gagggccgct tcgtgcatgc
     5521 cccgaacagc ggcggcaccg ttcgcctgga ccggctcgac ggcagctggt ggcgcgacca
     5581 ctacaccggt gcaaggcgat tgctgaattg aatcaacggc ttgggccctg ccgcctttca
     5641 taaacctgaa tgcgccgaag cgtcactttt gtgaccggtc ggcgcattca tttgcgtcca
     5701 gctaacgttt tttgaaccct tcacccggct gtcacgggca tagtcgccgc tggtttcaaa
     5761 tcgtgatgac cgcgtgacga cagcaaccct gccccacggc cataccgccg cccgccccgc
     5821 ccgccgggga tttaccctct tccttgccat ggcgcttgcc gcctgcgccg ccccggcgat
     5881 ggcgatcacc cccacctcgc cgccggccga tgccgcgccg caggtgaccg tcgccaccca
     5941 ggccccgctg gatgccgtgg ccccggtcga cctggccagc cccgacaacg ccccgctgct
     6001 cgcccagatc gccgccctgc agggcgcaca gaacgcgccc agcacctcgt cccggatcaa
     6061 gtcggtcctg cagcgcgcgt tttcgctgct gggcacgcct taccgctggg gcggtaccag
     6121 cccggaaggc ggtttcgact gcagcggcct ggtcggctac gtgttccgca ccatcggcat
     6181 cgacctgccg cgggtctccc gctcgatggc gcaggaaggc accgcggtga ccgatcgcac
     6241 tgccctggcc gaaggcgacc tggtgttctt cggcaagcgc ggccgtgtcg accatgtcgg
     6301 catctacatc ggcgaaggca agttcctgca tgccccccgc accggccggg acgtgaccgt
     6361 gtccagcctg gacagcggct actggagcca gaagtacctg caggcccggc gcctcgccac
     6421 cggcgcctga gccggcccac gcgatccgtc gaagacgccg cccgcgggcg gcgtcctgcg
     6481 tttcagggct tcctgccggc ttcgcggtag cgcgccacgg tctgttcgat gtccttgctc
     6541 agcgccatca cctgcatcgc gtattccgcc agccggtggt cctcctcgct gtcgggggtc
     6601 caggccggca ccgcgaccgg cgtgccctcc acgccatcca gcgccacgaa cacgatcacg
     6661 caatgcgtgc acaggcgctc gccggcatcc tgcatcgggt cgcgcgcgcg tacgtccacc
     6721 gcgaagtgca tcgagctggt gccggtgtgg accagcgtgg tttccaccgt gaccaggtcg
     6781 ctgatccgga tcggcgcgac gaagcggatc ccgcccaccg cgacggtgac gctgtacctg
     6841 ccgctccagc ccaccgccgc cgc
//
LOCUS       DAOOCX010000116         6822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_10445_length_6822_cov_7.936456, whole genome shotgun sequence.
ACCESSION   DAOOCX010000116 DAOOCX010000000
VERSION     DAOOCX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10445_length_6822_cov_7.936456"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PK399_09850"
     CDS             complement(<1..902)
                     /locus_tag="PK399_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPW13461.1"
                     /translation="MNAQLKTFSTEGDYKVADISLADWGRKELDIAEHEMPGLMSIRK
                     KYSASKPLNGVRVTGSLHMTIQTAVLIETLKDIGADVRWASCNIFSTQDHAAAAIAAT
                     GTPVFAWKGETLEEYWDCTLDALTFPGGKGPELVVDDGGDVTLLIHKGYELENGSTWV
                     DEPASSHEESVIKALLKRVAKERPGFWHTVVKDWKGVSEETTTGVHRLYQMVENKSLL
                     VPAINVNDSVTKSKFDNLYGCRESLADGLKRAMDVMLAGKVAVVCGYGDVGKGSAHSL
                     RAYGARVVVTEIDPINALQASMEG"
     regulatory      complement(949..1040)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(1109..1849)
                     /locus_tag="PK399_09855"
     CDS             complement(1109..1849)
                     /locus_tag="PK399_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020684101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HPW13462.1"
                     /translation="MTYCIGLNLDEGLLFASDSRTNAGVDDVRRFGKMRVFAVDGDRV
                     IVTLSAGNLSLTQNTLNLLEYRANHGDGVGLYNAQSMFEVASLVGDAMREIRKRDAPY
                     LRDTGIDASGTFIVGGQIKGEVPRLFLVYSEGNFIESNPDTPYFQTGEIKFGKPILDR
                     MVTPQTSLDQATKCALVSFDSTMRSNLSVGPPIDLLRYRRDALSIDLKMRLEDDDPYL
                     NQVRSHWNDSLRSALESLPTPAWMAPRG"
     gene            complement(1921..2919)
                     /locus_tag="PK399_09860"
     CDS             complement(1921..2919)
                     /locus_tag="PK399_09860"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HPW13463.1"
                     /translation="MLCRTASDLYWAARNVERAENTARLVDVTLRIALLPERYDRGRK
                     EAAPWRRALDALGLADVVRNRYGRIDAESVQRHLLLSAENPSSVYSCLHAARECARAQ
                     RVAITAEMYEDLNVSWLEMRGQTWSRLHADGVNNMLERVKGRSASFRGVTIGTLGRGE
                     GYHFLQLGAFVERAEWAIRLLDIAGSEGDDTETREQAAVDYFRWSALLQSLSAFETYR
                     RLYRGAVNAAGVVDLMLLQAGNPRSLLTCATTAHNVLQTLSGDDSLEVVRQAGALSAH
                     CRYARIDEILQQGLEPWLQAAMARLDRLGNAIHRQFMVSTDVATPVMAARPWSHEQ"
     gene            complement(3074..4522)
                     /locus_tag="PK399_09865"
     CDS             complement(3074..4522)
                     /locus_tag="PK399_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HPW13464.1"
                     /translation="MAIAYDEMHDPLGRVRAHYGAFDNWLNRTPETEIAHKRREAEIS
                     FHRVGITFSVYGEESGNERLIPFDLVPRIVPAVEWRMLEAGLRQRVHALNLFLGDVYG
                     EQRILKEGRVPRDLVLDNSEYRQEMRGFEVPGGVYSHISGIDLVRAGDGEYFVLEDNL
                     RVPSGVSYMLENRRMMARLLPDLFARQQIAPVERYPDMLLDTLREAAPAGVDYPVVAV
                     LTPGAGNSAYFEHAFLAQQMGVELVEGADLFVRDAMVYMRTTRGPQRVHVLYRRINDD
                     YLDPRVFRAESMLGVPGLFEAYRAGNVTLANAPGTGVGDDKSVYPYVPEMIRFYLSEE
                     PILHNVPTRILRDPEDLKYTLEHLHELVVKEVHGAGGYGMLVGPASTKAEIEEFRLRI
                     LADPGNYIAQPTLSLSTCPTFVESGLAPRHIDLRPYILCGRRINVVPGGLTRVALREG
                     SLVVNSSQGGGTKDTWVLESDGGQGLVVGERT"
     gene            complement(4610..6157)
                     /gene="bchE"
                     /locus_tag="PK399_09870"
     CDS             complement(4610..6157)
                     /gene="bchE"
                     /locus_tag="PK399_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     anaerobic oxidative cyclase"
                     /protein_id="HPW13465.1"
                     /translation="MRILLLNPPHTAIGSRIPREHLPPLGLLSVGGPLLDAGFDVELL
                     DAEFGPMAVGAIIDEIARRAPDLLLVGHSGSTSAHPTIALIAARIKARMPALRIVYGG
                     VFPTYHWHDVLEQEPAIDFVVRGEGERTALRLVQAIAARGDPASVPGIAYRSEGRVVT
                     TGQAEMLANLDDARVGWELIDHDRYSYWGGRKAVVVQFSRGCPYLCSYCGQRGFWTRW
                     RHRDPVLFAAELARLHREHGVQVINFADELPTGSRKAWKAFLEALVAENVPLTLVGST
                     RAGDIVRDADLLPLYKRAGVIRFLLGIESYSQATLAAIRKGADIDEDREAIRLMRQHG
                     IVSMATYVMGFEEETDRDYWNSLRHLLRYDPDQVQLMYVTPHRWTPYFDTVAARRVIE
                     PDVRKWDYKHQVLDTRRVPPWRVLLWVKFIEAVMQLRPRALFRNLLHPDPEVRYAMRW
                     YTRMGRRVWPHELWDFLSGKRLRRGPRLDAFWGPSLAEHEYALAKPARRNGPSVSRPT
                     IPLATVD"
     gene            complement(6332..>6822)
                     /locus_tag="PK399_09875"
     CDS             complement(6332..>6822)
                     /locus_tag="PK399_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HPW13466.1"
                     /translation="CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVA
                     KNIVAAGLADRCEVQVSYAIGVAEPTSISVTTFGTGKVGDDVIEKLIRSNFDLRPYGI
                     MQMLDLEHPIYQPTAAYGHFGRKPKEISYTDGAGKKQAATAFSWEKTDKADGLRKAAK
                     LK"
BASE COUNT         1063 a         2250 c         2339 g         1170 t
ORIGIN      
        1 aagccttcca tcgacgcctg gagggcgttg atcgggtcga tctcggtgac cacgacgcgc
       61 gcgccatagg cacgcagtga gtgcgccgag cccttgccga cgtcgccgta gccgcagacc
      121 accgcgacct tgccggccag catcacgtcc atcgcgcgct tcaggccatc ggccagcgac
      181 tcgcggcagc cgtacaggtt gtcgaacttg gacttggtga ccgaatcgtt gacgttgatg
      241 gccgggacca gcagcgactt gttttccacc atctggtaca ggcggtgcac gccggtggtg
      301 gtctcctccg agacgccctt ccagtccttg accacggtgt gccagaagcc cgggcgctcc
      361 ttggcgacgc gcttcagcag cgccttgatc acgctttcct cgtgcgagga ggcgggttcg
      421 tcgacccaag tggagccgtt ttcgagctcg tagcccttgt ggatcagcag ggtgacgtcg
      481 ccgccgtcat cgaccaccag ctccggaccc ttgccgcccg ggaaggtcag cgcgtccagc
      541 gtgcagtccc aatactcttc cagggtctcg cccttccacg cgaacaccgg ggtgccggtg
      601 gcggcgatcg cggcggcggc gtggtcctgg gtcgagaaga tgttgcaact ggcccagcgc
      661 acgtcggcgc cgatgtcctt cagggtctcg atcagcaccg cggtctggat ggtcatgtgc
      721 agcgagccgg tcacgcgcac gccgttgagc ggcttgctgg ccgaatattt cttgcggatc
      781 gacatcaggc ccggcatctc gtgctcggcg atgtccagtt ccttgcggcc ccaatcggcc
      841 agcgagatgt cggcgacctt gtagtcgccc tcggtcgaga aggtcttcag ttgtgcgttc
      901 atgtgtagct ccgtcgtcgg gagtcgcgat gcgcagggca tggactcgca gtcacgggcg
      961 ccgttgcagg aaaccttgcc gagcctgatt ccgggcagag cgccttggcg gcggccggac
     1021 ttgcagcgcc cctcggcagg gcgcgcgcag tttatcgcct tatcgggttt aaaggatgaa
     1081 tcgcgtggtc gccgggccga ccgacggttc agccgcgcgg ggccatccac gccggcgtgg
     1141 gcagcgattc cagcgccgag cgcaggctgt cgttccagtg gctgcggacc tggttgaggt
     1201 acgggtcgtc gtcttccagg cgcatcttga ggtcgatgga cagggcgtcg cggcggtagc
     1261 gcagcaggtc gatcggcggg ccgaccgaca ggttggaacg catggtcgag tcgaacgaca
     1321 ccagcgcgca cttggtggcc tggtcgagcg acgtctgcgg ggtgaccatg cggtccagga
     1381 tcggcttgcc gaacttgatc tcgccggtct ggaagtaggg cgtgtccggg ttggactcga
     1441 tgaagttgcc ttccgagtac accaggaaca gccgcggcac ctcgcccttg atctggccac
     1501 cgacgatgaa ggtgccggag gcatcgatgc cggtgtcgcg caggtagggc gcatcgcgct
     1561 tgcggatctc gcgcatcgca tcgccgacca ggctcgctac ttcgaacatc gactgcgcgt
     1621 tgtagaggcc gacgccgtcg ccgtggttgg cgcggtattc gagcaggttc agggtgttct
     1681 gggtcagcga caggttgccg gccgacaggg tgacgatgac ccggtcgccg tccactgcga
     1741 acacccgcat cttgccgaag cggcggacat cgtcgacccc tgcattggtg cgggaatcgg
     1801 aggcgaacaa caggccttcg tcgaggttca ggccgatgca ataggtcatg tcgggaggcg
     1861 tcgccggagt ggggtcggtg cgcgcgcatt gtgcgcgcgc attcgaccgt gggtgaaggg
     1921 ttactgttcg tggctccagg gacgcgcggc catcaccggc gttgcgacat cggtggagac
     1981 catgaattgc cggtggatgg cgttgcccag ccggtccagc cgcgccatcg ccgcctgcag
     2041 ccagggttcc aggccttgct ggaggatttc gtcgatgcgc gcgtagcggc aatgcgcgga
     2101 cagcgcgccg gcttgccgca ccacttccag gctgtcgtcg ccggacaggg tctgcagcac
     2161 gttgtgcgcg gtggtcgcgc aagtcagcag cgatcgcgga ttgcctgctt gcagcagcat
     2221 caggtcgacc acgccggccg cgttcaccgc gccgcgatac agccgccgat aggtctcgaa
     2281 tgccgacagc gattgcagca atgcgctcca gcggaaatag tcgacagcgg cctgttcgcg
     2341 tgtctcggtg tcgtcgccct cgctgccggc gatgtcgagc aggcggatcg cccactcggc
     2401 gcgttcgacg aaagcgccca gctgcaggaa gtgatagccc tcgccgcgcc ccagggtgcc
     2461 gatggtgacc ccgcggaacg acgccgaacg tcccttcacc cgttccagca tgttgttgac
     2521 gccatcggca tgcagcctgc tccaggtctg gccgcgcatc tccagccagg agacgttgag
     2581 gtcctcgtac atctccgcgg tgatcgcgac gcgttgcgcg cgtgcgcatt cgcgcgcggc
     2641 atgcaggcag ctgtacaccg acgacgggtt ctccgccgac agcagcaggt gccgctgcac
     2701 ggattcggca tcgatgcgcc cgtaacgatt gcgcaccacg tcggccaggc cgagtgcgtc
     2761 cagtgcgcgt cgccacggcg cggcttcctt cctgccgcgg tcgtagcgtt cgggcagcag
     2821 ggcgatgcga agggtcacgt cgaccaggcg tgcggtgttc tccgcgcgct cgacgttgcg
     2881 cgcggcccag tacagatcgc tggcggtgcg gcacaacatc aaagggcttg ccgtcggtga
     2941 acgcacaagg cgcgcggctt cgccgcccta cgggccgctg ctatccgcgc gcggcgcggc
     3001 gtcatcactc ggtcacgtat cgacatacgt tccctccttc ttccttgcgt cgcacgcgga
     3061 tagcggcggc gcgtcatgtg cgttcaccga cgacaagccc ttgccctcca tccgattcca
     3121 gtacccaggt gtccttggtg ccgccgccct gcgaggaatt caccaccagc gagccctcgc
     3181 gcagggcgac gcgggtcagt ccacccggca ccacgttgat ccggcgtccg cacaggatgt
     3241 agggacgcag gtcgatgtgg cgcggtgcca gcccgctctc gacgaaggtg gggcaggtcg
     3301 acaacgacag cgtgggctgg gcgatgtagt tgccggggtc ggcgaggatg cgcaggcgga
     3361 attcctcgat ctcggccttg gtggatgccg gcccgaccag catcccgtag ccgccggcgc
     3421 cgtggacttc cttcaccacc agttcgtgca agtgctccag cgtgtacttg aggtcctccg
     3481 gatcgcgcag gatgcgggtc ggcacgttgt gcaggatcgg ctcctcgctc aggtagaagc
     3541 ggatcatctc cggcacgtac ggatacaccg acttgtcgtc gcccacgccg gtgccgggcg
     3601 cgttcgcaag ggtcacgttg ccggcgcgat aggcctcgaa caggccgggc acgcccagca
     3661 tcgactcggc gcggaacacc cgcggatcca ggtagtcgtc gttgatccgc cggtacagca
     3721 cgtgcacgcg ctgcgggccg cgggtggtgc gcatgtagac catcgcatcg cgcacgaaca
     3781 ggtccgcgcc ctcgaccagt tccacgccca tctgctgggc caggaacgca tgctcgaagt
     3841 aagcggagtt gcccgcgccc ggggtcagca ccgccaccac cggatagtcc acgccggccg
     3901 gcgcggcctc gcgcagggtg tccagcaaca tgtccgggta gcgctcgacc ggcgcgatct
     3961 gctggcgcgc gaacaggtcc ggcaacagcc gcgccatcat ccggcggttc tccagcatgt
     4021 acgacacccc ggacggcacc cgcaggttgt cctccagcac gaagtactcg ccatcgccgg
     4081 cacggaccag gtcgatcccg gagatgtgcg aatacacgcc gccgggcact tcgaaaccgc
     4141 gcatctcctg ccggtattcg ctgttgtcga gcaccaggtc gcgcggcacg cggccttcct
     4201 tgaggatccg ctgctcaccg tagacatcgc ccaggaacag gttcagcgca tgcacgcgtt
     4261 ggcgcagccc ggcttccagc atccgccatt cgacggcggg gacgatccgc ggcaccaggt
     4321 cgaaggggat caggcgctcg ttgccggatt cctcgccgta caccgagaaa gtgatgccga
     4381 cccggtggaa ggaaatctcc gcctcgcgcc gcttgtgcgc gatttcggtt tccggcgtgc
     4441 ggttgagcca attgtcgaag gcgccgtagt gcgcgcgcac ccggcccagc gggtcatgca
     4501 tctcgtcgta cgcaatcgcc atccgactgg ccagcctgtg tccccgggtc gatggttccg
     4561 tgatagcacg cgggatggtg cggtgcaata acgggtcggc gcagcgggct cagtccaccg
     4621 tagccaacgg tatcgtcggc cgggacacgc ttgggccgtt gcgtcgcgcc ggtttcgcaa
     4681 gcgcgtattc gtgctcggcc agcgatggtc cccagaacgc atccaggcgc gggccgcgac
     4741 gcaagcgctt gccggagagg aagtcccaca gttcatgcgg ccacacgcgc cttcccatgc
     4801 gcgtgtacca gcgcatcgcg taacgtactt cggggtccgg gtgaagcagg ttgcggaaca
     4861 gggcacgtgg ccgcagttgc atcaccgcct cgatgaattt cacccacagc agcacgcgcc
     4921 acggtggtac ccggcgggtg tccagtacct ggtgcttgta gtcccacttg cgcacgtccg
     4981 gctcgatcac ccggcgcgcg gcgaccgtgt cgaagtaggg cgtccagcga tgtggcgtca
     5041 catacatcag ctgcacctgg tcggggtcgt agcgcagcag gtggcgcagc gagttccaat
     5101 agtcgcggtc ggtctcttcc tcgaagccca tcacgtaagt cgccatcgag acgatgccgt
     5161 gttggcgcat cagccggatc gcctcgcggt cctcgtcgat gtcggcgccc ttgcggatcg
     5221 cggcgagggt ggcctgcgaa tagctttcga tgccgagcag gaagcggatc accccggcgc
     5281 gcttgtacag cggcagcagg tcggcatcac gcacgatgtc gccggcgcgg gtggagccga
     5341 ccagggtgag cgggacgttc tccgcgacca gcgcttccag gaaggccttc cacgccttgc
     5401 gcgaaccggt cggcagttcg tcggcgaagt tgatcacctg cacgccatgc tcgcgatgca
     5461 gccgcgccag ctccgccgcg aacagcaccg gatcgcgatg gcgccagcgc gtccagaagc
     5521 cgcgctggcc gcagtagctg cacaagtagg ggcaaccgcg cgagaactgc accaccacgg
     5581 ccttgcgccc gccccagtac gaatagcggt cgtggtcgat gagctcccat cccacgcggg
     5641 catcgtcgag gttggccagc atctccgcct ggccggtggt gaccacgcga ccttcgctgc
     5701 gataggcgat gccgggcacg ctggccggat cgccgcgcgc ggcaatggct tgcaccaagc
     5761 gaagcgcggt gcgctcgcct tcgccgcgca cgacgaagtc gatggccggt tcctgttcca
     5821 gcacatcgtg ccagtggtag gtcgggaaca cgccgccata gacgatgcgc agggccggca
     5881 tgcgcgcctt gatccttgcg gcgatcagcg cgatcgtcgg atgcgcggag gtcgagccgg
     5941 aatggcccac cagcagcagg tcgggcgcac ggcgcgcgat ctcgtcgatg atcgcgccga
     6001 ccgccatcgg cccgaattcg gcatccagca attcgacgtc gaagccggca tccagcagcg
     6061 gcccgccgac gctcaacagg ccgagcggcg gcaggtgttc gcgcgggatg cggctgccga
     6121 tcgcggtatg cggtggattg agcaggagga tgcgcatgca gggcccgggc cgtcggcgct
     6181 ccgcgaggcg ggagcaccgc cagggtcgac gcgctgcatg cgcaggcgaa ggcgttggcg
     6241 tgaagcgggg ttgaagcgcg gggcaccccc gaaacgaaac gggccgcatc gctgcggccc
     6301 gtccgggttt gccttgcaac gcgcgcggcg cttacttcag cttcgccgcc ttgcgcaggc
     6361 cgtcggcctt gtcggtcttc tcccaggaga aggcggtggc ggcttgcttc ttgccggcgc
     6421 cgtcggtgta gctgatctcc ttcggcttgc ggccgaagtg gccgtacgcc gcggtgggct
     6481 ggtagatcgg gtgctccagg tcgagcatct gcatgatccc gtacgggcgc aggtcgaaat
     6541 tgctgcggat cagcttctcg atgacgtcgt cgccgacctt gccggtgccg aaggtggtga
     6601 ccgagatgct ggtcggttcg gccacgccga tggcgtagct gacctgcact tcgcaacggt
     6661 cggccaggcc ggcggcaacg atgttcttcg ccacgtagcg cgcggcgtag gcggccgagc
     6721 ggtcgacctt ggacggatcc ttgccggaga acgcaccgcc gccgtggcgg gccatgccgc
     6781 cgtaggtgtc gacgatgatc ttgcggccgg tcaggccgca gt
//
LOCUS       DAOOCX010000117         6790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_10504_length_6790_cov_9.883742, whole genome shotgun sequence.
ACCESSION   DAOOCX010000117 DAOOCX010000000
VERSION     DAOOCX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10504_length_6790_cov_9.883742"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PK399_09880"
     CDS             <1..307
                     /locus_tag="PK399_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPW13467.1"
                     /translation="PWGEKALTAYLGGDRATWSAWDACALVEQASEKLPLLVDQGGDD
                     EFLDGQLRPQLLQAAAVAARHPLQLRMHPGYDHSYYFIASFIGEHVAHHAAALRGGA"
     gene            complement(304..918)
                     /locus_tag="PK399_09885"
     CDS             complement(304..918)
                     /locus_tag="PK399_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPW13468.1"
                     /translation="MLVGRAMGFLIFSSRRGGLSRHGDGLVALAATLIAYAATEMAHG
                     YGFLAVFVCALTIRARERHHEFHEELHHFSDQVERLLMLLLLVLFGGALARGLLDALT
                     LQDALVCLAILLLIRPLAGMLAMAGSSHPPRERWLLAFLGIRGIGSMYYVAYGINHGR
                     FGDSERLWAAVGFLVLASIIIHGLSSDALLRRLARRRAQADPAG"
     gene            complement(888..1556)
                     /locus_tag="PK399_09890"
     CDS             complement(888..1556)
                     /locus_tag="PK399_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPW13469.1"
                     /translation="MQVDPYIAFLFGIGGMVLLASWAPLGLKRSPLPMTLPIICVGLG
                     VLLFGTRVFSLDPSPRTWDTLTERMTELVVIVSLMGAGLKLDRPLGWRRWGTTWRLLA
                     IAMPLTIAATAWLGMVGLGFPLAMALLFGASMAPTDPVLASDVQVGPPGKGDGDEVRF
                     GLTSEAGLNDALAFPFVHLAILVAAGGLGAQRPSANGSGSTWSGSCWRAWAWACWSGA
                     RWAS"
     gene            complement(1556..2251)
                     /locus_tag="PK399_09895"
     CDS             complement(1556..2251)
                     /locus_tag="PK399_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW13470.1"
                     /translation="MTPALLSWSGGKDAAWALHRLRQRGELDVVGLVTTVTEGVERVS
                     MQGIRVDVLEQQARATGLPVLQARIPHACDNATYEAAFAAALRTAQDRWPGLRDIAFG
                     DLFLADIRAWRDASCARIGWRTHYPLFGSDTASLAREMIAGGLRASLCCIDTTRLDAR
                     FAGSDFDDVLLAGLPPGIDPCGEHGEFHTCAWAGPMFAAPLDLQRGETVLRDDRFAYV
                     DFALRGASTTTTH"
     gene            complement(2269..2613)
                     /locus_tag="PK399_09900"
     CDS             complement(2269..2613)
                     /locus_tag="PK399_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13471.1"
                     /translation="MATRYYINLPDPAHARGNDPDLAFRAQGAEALATELEDALRGDG
                     LFERWRAKQEDPDAVDPSLGATDAGATVIGTQDDLHVSLVVTTALPSSVLRQRLGLLA
                     GSGWQLRDVTSA"
     gene            complement(2687..3628)
                     /locus_tag="PK399_09905"
     CDS             complement(2687..3628)
                     /locus_tag="PK399_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPW13472.1"
                     /translation="MRIGIVADSACDLPADFIEREQITILPVTIQIGQAVLADTRNDE
                     ATMNFLSGETAARAFEAESTPFTVQQVHDLFLTKLVHDYDYVFCITTTRTRSGIYDNA
                     VQASYTILNDYKPVRAASGNTTPFALRVIDSQNVFAAVGVLVVEAARLRAAGETPPKI
                     RARLENLALYTQGYMVPPDLHYLRNRIKKRGDKSVSFLSAALGTALDIKPILHCNRGD
                     TAPVGKVKGLGNAVEKMLDFASSRVRAGLMTPTVGLCYGGDLDEMRAMPGYARLRETC
                     AEHNVEVFETFMGLSGLVNVGKGAMVVAFAAEAHKFE"
     gene            complement(3779..5074)
                     /locus_tag="PK399_09910"
     CDS             complement(3779..5074)
                     /locus_tag="PK399_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW13473.1"
                     /translation="MSRSPMKRLALVAALLAASGLASAQDLLIRNATVHTASTQGTLR
                     ATDVLVRNGRIAAIGSGLAAGNATVVDAQGQPLTPALFGGITDIGVEEVSGESSTVDA
                     ALALGSGASDMQVRPEFDVTLAYNPESILVPVTRVEGFGWTLLSAGSRPGGSLVAGQG
                     GVVRLDGSLDAVGPRALFVTLGGDGANLSGSSRAAQWMLLDQLIDEVRGRIPADSQFA
                     LLTPAGRTTLAKYVGGGGRVMVRIQRASDIVRVLRWAKARDVRIALVGATEAWKVAPQ
                     LAAANVPVFVDALANLPADFDELGATLENAARLHAAGVKVSFFQTGDASHNARKVRQL
                     AGNAVANGLPWDAGLAGLTTVPASALGVGGEIGNTIAVGQRADLVLWSGDPLDVANVA
                     RQVWLDGRAIPMRSRQTELRDRYLHPDTGLPRTYPSDAR"
     gene            complement(5071..6471)
                     /locus_tag="PK399_09915"
     CDS             complement(5071..6471)
                     /locus_tag="PK399_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPW13474.1"
                     /translation="MSKPMTAALAAVLLAGCATTPATKPATGAAKPDLGFAADPYPST
                     YARVIGPAVLLQHATVLTGTGTRLDDADLLMRDGKIVAVGNAIDAPADAVRVDATGKW
                     VTPGIIDIHSHLGVYPSPGMSAHSDGNEATSPVTAQVWAEHSIWPQDPGFHAALAGGV
                     TSLQILPGSANLIGGRGVTVKNISSVTYQGMKFPGAPWGLKMACGENPKRVYGSKGGP
                     STRMGNIAGYRAAFADAAEYMKKRKDAADKPEGAGKRDLKLDTLAGAINGDIRVNIHC
                     YRADEMAIMLDLAKEFGFRISAFHHGVEAYKIADRLAAEGVCGALWADWWGFKMESFD
                     GIQENIALVDRAKNGCAIVHSDSEEGIQRLNQEAAKVIANAGRVGIAITPEHAIQWLT
                     TNPAKAMGILDQTGTLEAGKMADVVLWNGNPFSSYAQAEQVYVDGARLYDRRDRSRQP
                     TSDFLLGQDAARGGVR"
     gene            6592..>6790
                     /locus_tag="PK399_09920"
     CDS             6592..>6790
                     /locus_tag="PK399_09920"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410993.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HPW13475.1"
                     /translation="MKSKQDIVDNWLPRYTGVPLGGFGEHILLTNFGGYLGHFSEMTG
                     APVVGLDRPMPSATADGITMIN"
BASE COUNT         1053 a         2329 c         2361 g         1047 t
ORIGIN      
        1 gccatggggc gagaaagccc tcaccgccta cctgggcgga gatcgggcga catggagcgc
       61 ttgggatgcg tgcgctctgg tggagcaggc atcggaaaaa ttgccgctgc tggtcgacca
      121 gggcggtgat gacgaattcc tcgacggcca gctgcgcccg caactgctgc aggcggccgc
      181 ggtcgccgcc cggcatccgc tgcaactgcg catgcacccg ggctacgacc acagctacta
      241 cttcatcgcc agcttcatcg gcgagcacgt tgcccaccat gcggccgcat tgcggggcgg
      301 tgcctagccg gcgggatcgg cctgcgcccg cctgcgcgcc aatcggcgca acagggcatc
      361 ggaactcagg ccgtggatga tgatcgaggc cagcaccagg aagccgaccg cggcccacag
      421 gcgctcgctg tcgccgaagc ggccgtggtt gatgccgtag gcgacgtagt acatcgagcc
      481 gatcccgcgg atgccgagga atgccagcag ccagcgttcg cgtggcggat gcgatgatcc
      541 cgccatcgcc agcatgccgg ccagcggcct gatgagcagg aggatcgcca ggcagaccag
      601 cgcatcctgc agcgtcaatg catccagcaa tccgcgcgcc aacgcaccgc cgaacagcac
      661 cagcaggagc agcatcagca ggcgctccac ctggtcggaa aaatgatgca gctcctcgtg
      721 gaattcgtgg tggcgttcgc gcgcgcggat ggtcagcgcg cacacgaaca cggccaggaa
      781 gccgtagcca tgcgccatct cggtcgctgc ataggcgatc agggtggcgg ccagcgccac
      841 cagcccatcg ccgtggcgcg acaatccgcc gcggcggctg ctgaagatca ggaagcccat
      901 cgcgcgcccg accagcatgc ccatgcccag gcccgccaac agcttccaga ccacgtagat
      961 cccgaaccat tcgccgaggg tcgctgcgcc cccagtccac cggcggcgac caggatcgcg
     1021 aggtggacga acgggaaggc gagcgcatcg ttgaggcccg cctccgaggt caggccgaag
     1081 cgcacctcgt cgccgtcgcc cttgccgggc ggcccgacct ggacgtcgga cgccagcacc
     1141 gggtcggtgg gcgccatcga ggcgccgaac agcagtgcca tcgccagcgg gaatcccaag
     1201 cccaccatgc ccagccacgc ggtggcggcg atggtcagcg gcatcgcgat cgccagcagc
     1261 cgccaggtgg tgccccagcg ccgccagccc agcggccgat ccagcttcag gcccgcgccc
     1321 atcaggctga cgatcaccac cagttcggtc atgcgttcgg tcagcgtgtc ccacgtgcgc
     1381 gggctgggat ccaggctgaa gacacgcgtg ccgaacagca ggacgccgag ccccacgcag
     1441 atgatcggca gggtcatcgg caatggcgag cgcttcaagc caagcggtgc ccacgaggcc
     1501 agcaggacca tgccgccgat gccgaacagg aaggcgatgt agggatcgac ctgcatcagt
     1561 gcgtcgttgt cgtgctggcg ccgcgcagtg cgaaatccac gtaggcgaag cgatcgtcgc
     1621 gcagcacggt ctcgccgcgt tgcaggtcga gcggcgcggc gaacatcgga cctgcccatg
     1681 cacaggtgtg gaactcgccg tgttcaccac agggatcgat gccgggcggt agcccggcga
     1741 gcaggacgtc gtcgaagtcg cttccggcga agcgcgcatc gagtcgggtg gtatcgatgc
     1801 agcagaggct ggcgcgcagt ccgccggcga tcatctcgcg ggcgagcgat gcggtatcgc
     1861 tgccgaacag cgggtagtgc gtacgccagc cgatgcgcgc gcaggatgca tcgcgccaag
     1921 ccctgatatc ggccaggaac aggtcgccga aggcgatgtc gcgcaggccg ggccagcgat
     1981 cctgcgcagt gcgcagggcc gccgcgaacg ccgcttcata ggtggcgttg tcgcacgcgt
     2041 gcggaatgcg ggcctgcaac accggcaggc cggtggcgcg cgcctgctgc tccagcacat
     2101 cgacgcggat gccctgcatc gacacgcgct cgacgccttc ggtgacggtg gtcaccaggc
     2161 cgaccacgtc gagttcgccg cgctggcgca gcctgtgcaa ggcccaggca gcatccttgc
     2221 cgccgctcca cgacagcagt gcgggcgtca tcggcaatgc gttgcgcgtc aggccgaggt
     2281 gacgtcgcgc agctgccagc cacttccggc gagcaggcca agccgttgcc gcaacacgga
     2341 ggacggcaac gcggtggtca ccaccaggct gacatgcagg tcgtcctgcg tgccgatcac
     2401 ggtggcgccg gcatccgtgg cgccaagcga cggatcgacc gcgtccggat cctcctgctt
     2461 cgcacgccag cgttcgaaca ggccatcgcc gcgcaatgcg tcctccagct cggtggcaag
     2521 cgcctccgcg ccctgcgcac ggaacgcgag gtcggggtcg ttgccgcgcg catgcgcggg
     2581 atcaggcagg ttgatgtagt agcgcgtggc catgtggact cgatggggac gggaatccac
     2641 gcagcgtagt cgcccggcgg ttagcgcggc gtaattgccg cgcgcgtcac tcgaacttgt
     2701 gcgcttccgc ggcgaacgca acgaccatcg cacccttgcc gacgttcacc agcccggaca
     2761 ggcccatgaa cgtctcgaag acttcgacgt tgtgctcggc gcaggtctcg cgcagccggg
     2821 catagccggg catggcgcgc atctcgtcga ggtcgccgcc gtagcagagg ccgacggtgg
     2881 gcgtcatcag gccggcgcgc acgcggctgg aggcgaagtc gagcatcttc tcgaccgcgt
     2941 tgcccagccc cttgaccttg ccgaccgggg cggtgtcgcc gcggttgcag tgcaggatcg
     3001 gcttgatgtc cagcgcggtg cccagtgcgg cgctgaggaa gctcacgctc ttgtcgccgc
     3061 gcttcttgat gcggttgcgc aggtagtgca ggtcgggcgg gaccatgtag ccctgggtgt
     3121 acagcgccag gttctccagg cgcgcgcgga tcttcggcgg cgtctcgccc gcggcgcgca
     3181 ggcgcgcggc ctcgaccacc agcacgccca ccgcggcgaa cacgttctgg ctgtcgatca
     3241 cgcgcagcgc aaacggcgtg gtgttgcccg acgccgcgcg caccggcttg tagtcgttga
     3301 ggatggtgta gcttgcctgc accgcgttgt cgtagatgcc gctgcgggtg cgcgtggtgg
     3361 tgatgcagaa cacgtaatcg tagtcgtgca ccagcttggt caggaacagg tcatgcacct
     3421 gttgcacggt gaacggggtg gactccgcct cgaacgcgcg cgcggcggtt tcgccgctga
     3481 ggaagttcat cgtcgcttcg tcgttgcgcg tatccgccag cacggcctgc ccgatctgga
     3541 tggtgaccgg caggatggtg atttgctcgc gctcgatgaa gtccgccggc aggtcacacg
     3601 ccgagtccgc cacgattccg atccgcattg ccttgctccc gtcccccgac tgttgcgccg
     3661 aacgtaccgc aactgcgcgc tggttgcatc tgtgcttggg tagaagccat gttgtcctgc
     3721 agcatgcgga tggctgcgat tcagctgccg gacgccgcag gacgatgggc cgggcgactc
     3781 agcgtgcgtc gctcggatag gtgcgcggca ggcccgtgtc cggatgcagg tagcggtcgc
     3841 ggagttcggt ctggcgcgag cgcatgggga tggcgcggcc gtccagccac acctggcggg
     3901 cgacgttggc cacgtccagc ggatcgccgc tccacagcac caggtcggcg cgctgcccga
     3961 ccgcgatggt gttgccgatc tcgccgccga cgcccagcgc gctcgccggc accgtggtca
     4021 gcccggccag tcccgcgtcc cagggcaggc cgttggcgac cgcattgccg gccagctggc
     4081 gcaccttgcg tgcgttgtgc gaggcatcgc cggtctggaa gaagctgacc ttcacgcccg
     4141 ccgcgtgcag gcgcgcagcg ttctccaggg tcgcgccgag ttcgtcgaaa tcggccggca
     4201 ggttggccag cgcatccacg aacaccggca cgttggcggc ggcgagttgc ggtgccacct
     4261 tccatgcttc ggtggcgcca accagggcga tgcgcacgtc gcgcgccttg gcccagcgca
     4321 gcacgcggac gatgtcggag gcgcgctgga tgcgcaccat gacccgtccg ccgccgccga
     4381 cgtatttcgc cagcgtggtg cggccggccg gggtgagcag ggcgaattgc gagtccgcgg
     4441 gaatgcgccc gcgcacttcg tcgatcagct ggtccagcag catccactgc gccgcacgcg
     4501 agctgccgct gaggttggcg ccatcgccgc ccagggtgac gaacagcgcg cgtggcccca
     4561 ccgcatccag gctgccgtcc agccggacca cgccgccctg gcccgcgacc agcgacccgc
     4621 cgggccggct gcccgccgac agcagcgtcc agccgaagcc ttccacgcgc gtcaccggga
     4681 ccaggatcga ctcggggttg taggccaggg tgacgtcgaa ttccgggcgc acctgcatgt
     4741 cgctggcgcc gctgccgagc gcgagcgcgg catcgaccgt ggacgactcg ccggagactt
     4801 cctccacgcc gatatcggtg atgccgccga acagcgccgg cgtcagcggc tggccctgcg
     4861 catcgaccac ggtggcattg cccgcggcca ggccgctgcc gatcgcggcg atgcggccgt
     4921 tgcgcaccag cacgtcggtg gcacgcaggg tgccctgcgt gctggcggta tgcacggtgg
     4981 cgttgcggat cagcaggtcc tgcgctgagg cgaggccgct cgcggcgagc agcgcagcga
     5041 ccaaggcgag gcgtttcatc ggcgagcggc tcatcgcaca cccccacgcg ccgcgtcctg
     5101 cccgagcagg aagtccgagg tcggttgccg cgaacgatcg cgtcggtcgt acaagcgcgc
     5161 gccgtccacg tacacctgct cggcctgcgc atagctgctg aagggattgc cgttccagag
     5221 caccacgtcg gccatcttgc cggcctcgag cgtgccggtc tggtcgagga tgcccatcgc
     5281 cttcgccgga ttcgtcgtca accactggat cgcatgttcc ggggtgatgg cgatgccgac
     5341 gcggccggca ttggcgatca ccttggccgc ttcctggttg agtcgctgga tgccttcctc
     5401 ggaatccgaa tgcacgatcg cgcaaccgtt cttcgcccgg tcgaccaggg cgatgttctc
     5461 ctggatgccg tcgaagcttt ccatcttgaa gccccaccag tcggcccaga gcgcgccgca
     5521 cacgccttcc gcggcgagcc ggtcggcgat cttgtaggcc tccacgccgt ggtggaacgc
     5581 gctgatcctg aagccgaatt ccttcgccag gtcgagcatg atcgccatct cgtcggcgcg
     5641 gtagcagtgg atgttgacgc ggatgtcgcc gttgatcgca ccggccagcg tgtccagctt
     5701 gaggtcgcgc ttgcccgcac cctcgggctt gtcggcggcg tccttgcgct tcttcatgta
     5761 ctcggcggca tcggcgaagg ccgcgcgata gccggcgatg ttgcccatcc gtgtcgatgg
     5821 gccgcccttg ctgccgtaca cgcgcttggg gttctcgccg caggccatct tcaggcccca
     5881 cggcgcgccg gggaatttca tgccctggta ggtgacggac gagatgttct tcaccgtcac
     5941 cccgcgcccg ccgatcaggt tggcgctgcc cggcaggatc tgcaggctgg tcacgccgcc
     6001 tgcgagcgcc gcgtggaagc ccgggtcctg cggccagatc gaatgctcgg cccagacctg
     6061 cgcggtgacc ggcgaggtcg cttcgttgcc gtcgctgtgc gcactcatgc ccgggctggg
     6121 atacacgccc aggtgcgaat ggatgtcgat gatgccgggg gtgacccact tgccggtcgc
     6181 gtccacccgc actgcgtcgg cgggtgcgtc gatggcgttg ccgaccgcga cgatcttgcc
     6241 gtcgcgcatc agcaggtcgg cgtcgtccag gcgggtgccg gtgccggtca gcacggtggc
     6301 gtgctgcagc agcaccgccg ggccgatgac ccgcgcatag gtgctcgggt aggggtcggc
     6361 ggcgaagccg aggtcgggct tggcagcgcc cgtggcgggc ttggtggcgg gcgtggtggc
     6421 gcagccagcc agcagcaccg cggcgagcgc ggcggtcatc ggtttcgaca tgtggtttcc
     6481 ccatcgcacg gcgtgcgagc cagaacccta acccggccgg cgcccatccg ccacatgacc
     6541 gaggtcatgc ctgcgtggga acatggtgga tcgaaacacg gagaggaacg catgaagagc
     6601 aagcaggaca tcgtcgacaa ctggctgccg cgctacaccg gggtgccgct gggcggcttc
     6661 ggcgaacaca tcctgttgac caacttcggc ggttacctcg gccacttctc cgagatgacc
     6721 ggcgcgccgg tggtgggcct ggaccggccg atgcccagcg ccaccgccga cggcatcacc
     6781 atgatcaatt
//
LOCUS       DAOOCX010000118         6689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_10711_length_6689_cov_6.889659, whole genome shotgun sequence.
ACCESSION   DAOOCX010000118 DAOOCX010000000
VERSION     DAOOCX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10711_length_6689_cov_6.889659"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..1858
                     /gene="nirK"
                     /locus_tag="PK399_09925"
     CDS             329..1858
                     /gene="nirK"
                     /locus_tag="PK399_09925"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HPW13476.1"
                     /translation="MKAATLRLLTLAIAIAIGLPACSKDDAATQPNASAATTETETGG
                     SAKGDFGPPQGAPVEAVLTSPPHVPPATGRTAPAKVIVSLDVVEKDMEISEGVTYTFW
                     TFGGTVPGSFIRVRQGDTVEFHLRNMPDSKMPHNIDLHGVTGPGGGAASSFTAPGHVS
                     RFTFKALNAGLYVYHCATAPVGMHVANGMYGLILVEPPQGLPKVDREYYVMQGDFYTT
                     GKYREKGHQGFNMEKAIDENPTYVLFNGMEGSMTGDKALTAKTGETVRLYVGNGGPNL
                     VSSFHVIGEIFDKVWYEGGTKFQENVQTTLIPAGGAMMAEFHMEVPGSYVLVDHSIFR
                     AFNKGALAILKAEGPENKAIYSGKEVDAVYLGDQAAGTSRAPVAAAAASAKSGNLTKE
                     EQIAAGKVLFAGTCSTCHQPDGAGMPGVFPPLAGSDFLKANPKRLPEIILHGLVGPVT
                     VNGKDYNSNMPPMSQLTDDEVANIGTYVLNSWGNPGGQVSKADAAAARAAKPANSSDG
                     H"
     gene            1862..2644
                     /locus_tag="PK399_09930"
     CDS             1862..2644
                     /locus_tag="PK399_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HPW13477.1"
                     /translation="MRRVLAVALLAIPLAGLAAGALGSRYVGLPGGVFKSVLRYEDAK
                     DGVRIAPFALMRRPVTNTEFLDFVRTHPQWQRGKVAPVFAEARYLSHWAGPTTLGDNA
                     LPDRPVVWVSWFAADAYCDSIGARLPNWNEWEYAAAADETRRDARQDPAWRERILGWY
                     SRPSNTVLPRVGLQAPNAWGVQDLHGLVWEWTEDASSLLVDSDNRTQGDPDKAKFCGA
                     GALSMDDRENYAVMMRVAMLSSLDGHDATANMGFRCAWSAPR"
     gene            2641..3252
                     /locus_tag="PK399_09935"
     CDS             2641..3252
                     /locus_tag="PK399_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HPW13478.1"
                     /translation="MKRSNFATTARAAVVGIALAMGASPAFAGGPTARPLPSDSVYQL
                     ALPLTDQAGRTADWRNLRGKPRVVSMFYTSCQYICPLIVDSGKAIEHQLSPAERGRIG
                     IVLISMDPARDTPAALRKVFDKRGLDAAGWLLAAPPAEDVRAVAGLLGIRYRQLADGE
                     FNHTSALILVDAEGRIITRTEQVGSKPDPAFIEAVRREAAKRS"
     gene            3317..4711
                     /locus_tag="PK399_09940"
     CDS             3317..4711
                     /locus_tag="PK399_09940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW13479.1"
                     /translation="MPTSDTQTTPGRFQLQFLGANATVTGSRHLVTAGSSRILVDCGL
                     FQGYKPLRLRNWAEFPVDPASIDAVLLTHAHLDHSGYLPRLVRSGFRGKVWCTEATRS
                     LCGILLPDSARLLEEEAEHANRSGSSKHHPAEPLYREADARASLAHLHGVAFDRVFQP
                     APGVHARFRMQGHILGAAAATLEFEGRRITFSGDLGRPHDPLMPAPAALAESDWVVVE
                     STYGNRTHPAEAPDEELLAVLRRVIARRGVVVIPAFAVGRAQMLLHVITQLQETGQLA
                     RVPVYLNSPMAASVSGLYRTFPRLHRLDAHALAAMERNVHVVDSVEASKALNRRKGPM
                     IIVSASGMATGGRVLHHLLAFATEPRNAIVLCGFQAGGTRGAALAAGQKQLRIFGQEV
                     PVGAEVVQLAAGSAHADGDEILAWLRSAPTPPRKVFVTHGEPDAADALRARIEHELGW
                     PAHFPEYLEQVDLA"
     gene            4743..>6689
                     /locus_tag="PK399_09945"
     CDS             4743..>6689
                     /locus_tag="PK399_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPW13480.1"
                     /translation="MTGSLAGNRPTGHDAGPAAPPRRWAGLTSVEASARHLRDGPNAL
                     PQPERRSHWAIVLSVVREPMLLLLLAATTLYLVLGDTGEALLLALSVLAVIGLTIYQE
                     QKSERALEALRELGSPRARALRDGSPCLLPAGELVVGDVIFLAEGDRVPADARLLEAI
                     DLQVDESLLSGESVPLRRRVDGPGEEALVHASTLVVAGHGVAEVTATGPRTEVGKIGV
                     SLRSLHVEPTPMQREIRRVVLLFAGLALSTAVLVSGLFLASRGGLADAILAGLTLAIA
                     TIPEEFPVVLAVFLALGAWRMARHQALVRRTPAIEALGAITVLCADKTGTLTRNRMAV
                     AELVAGAQRSGVQPALPPDLRALLDAGVLASRADSIDPMDRALHEAAAALDPAASADG
                     APTQVLLREYPLSPACTAVTHAWQRGNGVFVACKGAPETVADLCHATAAQRAAMLADV
                     EAMGRRGLRVLAVAAAAWNDDAAALPSAMQGFAFAWLGLVGFADPLRDGVADAVAEAR
                     AAGIRVVMLTGDHRATARAIATQAGIAADGAVMEGRELDAMDDATMLRCARETNVFAR
                     VRPEQKLRLVAALKQDGQVVAMTGDGVNDAPALVSAHVGIAMGQRGTDVAREAAAIVL
                     LDDDFVTVVRAIRLGRTIYDNIQRA"
BASE COUNT         1046 a         2393 c         2319 g          931 t
ORIGIN      
        1 gtcgtcgacc tgttcgtggc gcagtcgcag gtcccattcg acgtggatgc cgggggtgtc
       61 ggccgccatc gcagcaaacg gtgcggcaca gcaacaggcg agcatcgaca acggaagggc
      121 gaggcgcggc gacatggcga tggctccgtt tggcgttgag gcgagcatcg tccgccgcct
      181 atggctcggc gttgatgcag gtcaagcacg cgcatgccgg gtcacatggc atggaggaac
      241 ttgatccaga tcaaagcggt aagctcagcc agggacggat gctgcaggca tcgacccaac
      301 gcgcacgcca tccaagggga acatcgccat gaaagccgcc acgctccggt tgctcacgct
      361 cgccatcgcc atcgccatcg ggctgcccgc ctgcagcaag gacgatgccg ccacccagcc
      421 caacgccagc gcagcgacga ccgaaaccga aaccggcggc tccgccaagg gcgacttcgg
      481 cccgccgcag ggcgcgccgg tcgaagcggt gctgacctcg ccgccgcatg tgccgcccgc
      541 gaccgggcgc accgcgccgg ccaaggtgat cgtctcgctg gacgtggtcg agaaggacat
      601 ggagatctcc gaaggcgtga cctacacctt ctggaccttc ggcggcaccg tgccgggcag
      661 cttcatccgc gtgcgccagg gcgacacggt ggagttccac ctgcgcaaca tgccggacag
      721 caagatgccc cacaacatcg acctgcacgg cgtcaccggg cccggtggcg gcgcggcctc
      781 cagcttcacc gcgcccggcc atgtcagccg cttcaccttc aaggcgctca acgccggcct
      841 gtacgtctac cactgcgcca ccgcgccggt gggcatgcac gtggccaacg gcatgtacgg
      901 gctgatcctg gtcgagccgc cgcaagggct gccgaaggtc gaccgcgagt actacgtgat
      961 gcagggcgac ttctacacca ccggcaagta ccgcgagaaa ggccaccagg gcttcaacat
     1021 ggagaaggcc atcgacgaga acccgaccta cgtgctgttc aacggcatgg aaggctcgat
     1081 gaccggcgac aaggcgctca ccgccaagac cggcgagacc gtgcgcctgt acgtgggcaa
     1141 cggcggcccg aacctggtgt cgagcttcca tgtcatcggc gagatcttcg acaaggtctg
     1201 gtacgagggc ggcaccaagt tccaggaaaa cgtgcagacc acgctgatcc ccgcgggcgg
     1261 cgcgatgatg gccgaattcc acatggaagt gccgggcagc tacgtgctgg tcgaccacag
     1321 catcttccgc gccttcaaca agggcgcgct ggcgatcctc aaggccgaag gcccggagaa
     1381 caaggcgatc tattccggca aggaagtcga cgccgtgtac ctgggcgacc aggccgccgg
     1441 caccagccgc gcgccggtgg ccgccgccgc ggcctcggcc aagtccggca acctgaccaa
     1501 ggaagagcag atcgccgcag gcaaggtgct gttcgccggg acctgctcca cctgccacca
     1561 gcccgacggc gccggcatgc ccggcgtgtt cccgccgctg gccggctccg acttcctcaa
     1621 ggccaacccg aagcgcctgc cggaaatcat cctgcacggc ctggtcgggc cggtgacggt
     1681 caacggcaag gactacaact ccaacatgcc gccgatgagc cagctgaccg acgacgaggt
     1741 cgccaacatc ggcacctacg tgctcaacag ctggggcaac ccgggcggcc aggtgagcaa
     1801 ggccgatgcc gccgcggcac gcgccgccaa gccggccaac agctcggacg gccattgagc
     1861 gatgcgtcgc gtactcgccg tcgcgttgct cgcgatcccg ctcgccggcc tggcggccgg
     1921 tgcgctgggg tcgcgctacg tgggcctgcc cggtggggtg ttcaagtcgg tgttgcgcta
     1981 cgaggacgcg aaggacggcg tgcgcatcgc gccgttcgcg ctgatgcggc ggcccgtgac
     2041 caacacggag ttcctggact tcgtgcgcac gcatccgcag tggcagcgcg gcaaggtcgc
     2101 gccggtgttc gccgaagccc gctacctctc gcactgggca ggcccgacca cgctgggcga
     2161 caacgccctg ccggaccggc ccgtggtctg ggtcagctgg ttcgccgcgg atgcgtactg
     2221 cgactccatc ggcgcgcgcc tgccgaactg gaacgaatgg gaatatgccg ccgcggccga
     2281 cgaaacccgc cgcgacgcgc gccaggatcc ggcctggcgc gaacgcatcc tgggctggta
     2341 ttcgcggccg tcgaacacgg tgctgccgcg ggtcggcctg caggcgccga atgcctgggg
     2401 cgtgcaggac ctgcacggcc tggtctggga atggaccgag gatgcatcgt ccttgctggt
     2461 ggacagcgac aaccgcaccc agggcgatcc cgacaaggcg aagttctgcg gcgccggcgc
     2521 gctgtcgatg gacgaccgcg agaactacgc ggtgatgatg cgggtggcga tgctgtcctc
     2581 gctggacggg cacgatgcca ccgccaacat gggcttccgc tgtgcctgga gcgcaccacg
     2641 atgaaacgat cgaatttcgc gacgacggcg agggccgcgg tggtcggcat cgccctggcc
     2701 atgggcgcat ccccggcctt cgccggcgga cccaccgcca ggccattgcc atccgactcc
     2761 gtctaccagc tcgccctgcc gctcaccgac caggccggac gcaccgccga ctggcgcaac
     2821 ctgcgcggca agccgcgggt ggtgtcgatg ttctacacgt cgtgccagta catctgcccg
     2881 ttgatcgtgg actccggcaa ggcgatcgag caccagttgt cgccggccga acgcggccgc
     2941 atcggcatcg tcctgatcag catggatccg gcgcgcgaca ccccggctgc cctgcgcaag
     3001 gtgttcgaca agcgcgggct ggatgccgcc ggctggttgc tggccgcgcc gcccgcggag
     3061 gacgttcgcg cggtcgccgg cctgctcggc atccgctacc ggcaactcgc cgatggcgag
     3121 ttcaaccaca ccagcgcgtt gatcctggtg gacgcggaag ggcgcatcat cacccgcacc
     3181 gaacaggtgg ggagcaagcc ggatccggcg ttcatcgaag cggtcaggcg cgaggccgca
     3241 aagcggtcct gagccatcgg cgagtaccct cggcgacgcg gcgcgcacgc ccctcccctt
     3301 gctgacgagt gccgacatgc caacgagcga cacccagacc accccgggcc gattccagct
     3361 ccagttcctc ggcgccaatg cgaccgtcac cggctcgcgc cacctggtga ccgcgggctc
     3421 gtcccggatc ctggtcgact gcggcctgtt ccagggctac aagccgctgc gcctgcgcaa
     3481 ctgggcggag ttcccggtgg acccggcctc gatcgatgca gtgctgctga cccacgcgca
     3541 cctggaccac agcggctacc tgccacgcct ggttcgctcc ggcttccgcg gcaaggtctg
     3601 gtgcaccgag gcgacccgca gcctgtgcgg catcctgctg ccggactccg cgagactgct
     3661 ggaggaggaa gccgagcacg ccaaccgcag cgggtcatcg aagcaccatc ccgcggaacc
     3721 gctttaccgc gaagccgacg cgcgcgccag ccttgcgcac ctgcatggcg tcgccttcga
     3781 ccgcgtgttc cagcccgccc ccggcgtcca cgcgcgcttc cgcatgcagg gtcacatcct
     3841 cggcgccgct gccgccaccc tggagttcga gggccgccgg atcaccttca gcggcgacct
     3901 gggccggccg cacgacccgc tgatgccggc gcctgctgca ttggcggagt ccgactgggt
     3961 ggtggtggaa tcgacctacg gcaaccgcac ccatcccgcc gaagcgcccg acgaggagtt
     4021 gctggcggtg ctgcggcgcg tgatcgcgcg aaggggcgtg gtggtcatcc cggcgttcgc
     4081 ggtcggccgc gcgcagatgc tgctgcatgt catcacgcaa ctgcaggaga ccggccagct
     4141 cgccagggta ccggtctacc tcaacagccc gatggcggcg agcgtcagcg gcctctatcg
     4201 cacgttcccg cgactgcatc ggctggacgc ccacgcgctg gcggcgatgg aacgcaatgt
     4261 ccacgtcgtc gacagcgtcg aggcatccaa ggcgctcaat cggcgcaagg gcccgatgat
     4321 catcgtgtcc gccagcggga tggccaccgg cgggcgcgtg ctccaccacc tgctggcatt
     4381 cgcgacggag ccgcgcaacg cgatcgtgct gtgcggattc caggccggcg gcacccgcgg
     4441 cgcggcgctg gccgcgggcc agaagcagtt gcgcatcttc ggacaggagg tgccggtcgg
     4501 cgccgaggtc gtgcagctcg ccgccggttc cgcgcatgcc gatggcgacg agatcctggc
     4561 ctggctgcgc agcgcgccga caccaccgcg caaggtcttc gtcacccacg gcgagccgga
     4621 tgccgccgat gcgctgcgtg cccgcatcga gcacgaactc ggctggccgg cacatttccc
     4681 ggagtacctc gaacaggtcg acctcgcctg aacacggcgc atcgacgcag ttggagcgcg
     4741 acgtgaccgg atcgcttgcc ggtaaccgcc ccaccggcca cgacgccgga ccggcagcgc
     4801 caccccggcg ctgggcgggc ctgacgtcgg tggaggccag cgcgcgccac ctgcgcgatg
     4861 gccccaatgc cctgccgcaa cccgaacggc gttcgcactg ggcgatcgtg ctcagcgtgg
     4921 tgcgcgaacc gatgttgctg ctcctgctgg ccgccaccac gctctacctg gtgctgggcg
     4981 acaccgggga agccctgctg ctcgcactgt cggtgctggc ggtgatcggc ctgacgatct
     5041 accaggaaca gaagtccgaa cgcgcgctgg aggccctgcg cgaactgggg agcccacgtg
     5101 cacgcgcgct gcgcgatggc agcccgtgcc tgctgcccgc cggcgaactg gtggtgggtg
     5161 acgtcatctt cctcgccgag ggcgaccggg tgcccgcgga cgcgcgcctg ctggaggcca
     5221 tcgacctcca ggtcgacgaa tcgctgttga gcggcgaatc cgtgccgctg cggcgtcggg
     5281 tcgatggccc cggcgaagag gcgctggtcc atgccagcac gctggtggtg gctggacacg
     5341 gcgtggccga agtcaccgcc accggcccgc gtaccgaggt cggcaagatc ggcgtctcgc
     5401 tgcgcagcct gcacgtggaa ccgacgccga tgcagcggga aatccgccgc gtggtgctgc
     5461 tgttcgccgg cctggcgctg tccaccgcgg tgctcgtctc gggattgttc ctggcaagcc
     5521 gtggcggact ggcggacgcg atcctggccg gcctgaccct ggcgatcgcc accatcccgg
     5581 aagaattccc ggtggtgctc gcggtgttcc tcgcgctggg cgcgtggcgg atggcgcggc
     5641 accaggccct ggtccggcgc accccggcga tcgaggcgct gggcgcgatc accgtgttgt
     5701 gcgccgacaa gaccggcacc ctcacccgca accgcatggc ggtcgccgag ctggtggccg
     5761 gcgcacaacg cagcggcgtg caaccggcgc tgccgcccga cttgcgcgcg ctgctcgacg
     5821 cgggcgtgct ggcgagccgc gcggattcga tcgaccccat ggaccgcgcc ctgcacgagg
     5881 ccgccgcggc actggacccg gccgcctccg cggatggcgc gccaacgcag gtgctgctgc
     5941 gcgaataccc gctgtcgccc gcctgcaccg cggtgaccca cgcctggcag cgcggcaacg
     6001 gcgtgttcgt ggcctgcaag ggcgcgccgg aaaccgtggc cgacctctgc catgccaccg
     6061 ccgcacagcg tgccgcgatg ctcgcagacg tcgaggcgat gggacgccgg ggcctgcgcg
     6121 tgctggccgt cgccgctgcc gcgtggaacg acgatgccgc cgccctgccc tccgccatgc
     6181 agggcttcgc cttcgcctgg ctgggcctgg tggggttcgc cgatccgctg cgcgacgggg
     6241 tcgccgatgc cgtggcggag gccagggctg ccggcatccg cgtggtcatg ctgaccggcg
     6301 accatcgcgc caccgcgcgg gcgatcgcga cgcaggcggg gatcgcggcg gacggcgcgg
     6361 tgatggaagg ccgcgagctg gacgcgatgg acgacgcgac gatgctgcgc tgcgcacgcg
     6421 aaacgaatgt gttcgcccgc gtgcgccccg agcagaagct gcggctggtc gccgcgctca
     6481 agcaggacgg tcaggtggtg gcgatgaccg gcgacggggt caacgatgcg cccgcgctgg
     6541 tgtccgcgca cgtcggcatc gcgatgggcc agcgcggcac cgacgtcgcc cgcgaggccg
     6601 cggcgatcgt gctgctcgac gacgacttcg tcaccgtggt gcgggcgatc cgcctgggcc
     6661 ggacgatcta cgacaacatc cagcgcgcg
//
LOCUS       DAOOCX010000119         6545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11033_length_6545_cov_7.677812, whole genome shotgun sequence.
ACCESSION   DAOOCX010000119 DAOOCX010000000
VERSION     DAOOCX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11033_length_6545_cov_7.677812"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="PK399_09950"
     CDS             <1..162
                     /locus_tag="PK399_09950"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HPW13481.1"
                     /translation="TPEGPGSLHIETPTFALTVIAVIVILTLLQFMPALVLGPVADHL
                     TLVHGGTTP"
     gene            159..2246
                     /gene="kdpB"
                     /locus_tag="PK399_09955"
     CDS             159..2246
                     /gene="kdpB"
                     /locus_tag="PK399_09955"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HPW13482.1"
                     /translation="MIQQTSEAKPRGQHPTVFDAAALRTAIRDAFLKLSPRHIVKSPV
                     MAIVLGGTLLSALITAAGAAPAGFGWSVTTILLVTVLFGNFAEAVAEARGRGQAASLR
                     RARKDLVARKLVEAKDAAPGHHGSEAQVPAAELRPGDLVVVSAGELIPADGEILHGLA
                     TINESAVTGESAPVLREAGTDRSGVIGGTKVLSDEIVVRVTVEPGHSFLDRMIALVEG
                     ANRQKTPNEIALTMLLAAMTLTFLIVCATLPAIGAFVGVKVDPLLLIALLVCLIPTTI
                     GGLLPAIGIAGMNRALSANVLAKSGKAVEVAGDVDVLLLDKTGTITHGDRQATAFHAL
                     AGISADQLREAALLSSLADPTPEGKSIVKLAREQGTPMQDPDQLDAGTTQFIPFTAQT
                     RMSGVDLDGGARLIRKGAGDAISRHVLSLGGDVPAELKARVDQVARKGATPLVVSEGR
                     FALGVVELSDVVKHGVKEKFARLRAMGIKTVMITGDNPLTAAAIAAEAGVDDYIAEAT
                     PEDKLARIRSEQAGGRLVAMVGDGTNDAPALAQADVGLAMNSGTQAAKEAGNMVDLDS
                     DPAKLLAVVEVGKQQLITRGALTTFSLANDVSKYFAILPALFAAAIPSMATLDVMGLS
                     SPKNAVLAALIFNALVIPALIPLALRGVRFAPASATVLLRRNMLLYGGGGVLLPFIAI
                     KLIDLALAATVGP"
     assembly_gap    2252..2351
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2352..2576
                     /locus_tag="PK399_09960"
     CDS             <2352..2576
                     /locus_tag="PK399_09960"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010381816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HPW13483.1"
                     /translation="PMELVTRSGGGLDPHLTPAGARVQVARVAKARGLDAGIVQQAVD
                     AATEAPQFGVLGQPRINVLLLNLALDARPR"
     gene            2596..5262
                     /locus_tag="PK399_09965"
     CDS             2596..5262
                     /locus_tag="PK399_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016849531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HPW13484.1"
                     /translation="MNADRSEVVRQAQADALLGELQRQGGGRLTIFLGAAPGVGKTYA
                     MLSRGRELKKQGTDVVVGLVETHGRVETQALLDGLDVLPRRRVEHQGRTLEELDLDAL
                     LARKPRVALVDELAHRNVPGSRHERRWQDVVELLDAGIDVYTTVNIQHLESLNDIIHR
                     ITGVRVSETVPDLVFDRLRDIKLIDLPPRELIERLQQGKVYMPDQAAHALQSFFSPSN
                     LAALRELAMQTAADRVDSDLREAQTARGLPGSSLRRRVLVAIDGRGQSDYLVRVARRV
                     AERRDAPWSVVSVQSGAHLDDAAQRELDRAFALARRLGGDAELLHGPSIVDALIDHAG
                     RSGVSTIVIGRTRERPFARMFNRTLTQQLLQRAAHLELTIVSTPEARAKSRRSLHDVG
                     ALLTRTDAGLAFAAASLATFTAWLAERFISLDDLSMVFIVAVVLVASRTRMTAAVLAA
                     GLCFLSYNFFFIEPRLTLFIGARQGVATVFLFLAAALVAGRLASRLRMQVVALRAANA
                     HATALQGLSRQLAAAADAGEVAEAGRRALKQALGADAIVQAGDVVAETPGQALLSDKD
                     RAAADWALKHRQPAGRFTDTLGNAEWWFLPLAGDKAATGLLGLRFPAGASRFGSEQRR
                     LVEAMADDIAQAMLRTRLVSDLEDARVTGETERLRSALLSSVSHDLRSPLAAIVGAAE
                     TLDTYGRGMGEDDRHSLLDTVRLEGERLDRYIQNLLDMTRLGHGGLTLNRDWIGVDEL
                     IGSAATRLQRYQPDVRFDIALAKGLAPLWVHPALVEQAIFNVIENAAKFSPAGEAIAI
                     DARDIDGVLRIDVADRGPGIPEDERKRIFDMFFSVERGDRGRQGTGLGLAITQGMIGA
                     HGGGVEALPGPDGRGTTIRITLPRMTPAAASA"
     gene            5259..5969
                     /locus_tag="PK399_09970"
     CDS             5259..5969
                     /locus_tag="PK399_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW13485.1"
                     /translation="MSETAASTAARILVVDDEPQIRKFLDISLRAQGHRVETAADGEA
                     GLQAMATHGADLVILDLGLPDLDGHEVLSRLREWSQVPVIVLTVRADEREKVAALDAG
                     ANDYVTKPFGVQELMARVRALLRSHATAGEAPPVFDDGRLRVDLARREVFLLGEPVQL
                     ARKEYALLALLLQHAGRVVTQPQMLRELWGRHHEHDTHYLRVLVGKLRQKLGDDAAAP
                     RWIATEPGVGLRFIGAPR"
     gene            complement(5993..>6545)
                     /locus_tag="PK399_09975"
     CDS             complement(5993..>6545)
                     /locus_tag="PK399_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HPW13486.1"
                     /translation="HPEHAGEWLRRWPLRKFPLLLDGERQVVETSIIIEYLQLAHPGA
                     VRLLPEDEISALDVRFMDRCFDLHVMSPVQAAVGGALTGDAIKRDDARAFAADKLEMA
                     YGWLEGELAGRAWACGEDFTMADCAAAPSLFYADWTHPIGDAYPVLRAYRARLLARPS
                     VARAVDEARPFRHLFPLGAPDRD"
BASE COUNT          989 a         2274 c         2232 g          950 t
ORIGIN      
        1 acgccggaag gccccggcag cctgcacatc gaaaccccga ccttcgcgct caccgtgatc
       61 gcggtgatcg tgatcctgac cctgctgcag ttcatgcccg cgctggtgct cggccccgtc
      121 gccgaccacc tgaccctggt gcatggagga accaccccat gatccaacag accagtgagg
      181 ccaagccgcg tggccagcat ccaaccgtgt tcgatgccgc cgccttgcgc accgcgatcc
      241 gcgatgcctt cctcaagctt tcgccccgcc acatcgtgaa gagtccggtg atggcgatcg
      301 tgctcggcgg caccctgctg tccgcgctga tcaccgccgc cggcgcggca ccggccggct
      361 tcggctggag cgtcaccacg atcctgctgg tcaccgtgct gttcgggaat ttcgccgaag
      421 cggtggcgga ggcgcgcggt cgcggccagg cggcgtcgct gcggcgtgcg cgcaaggatc
      481 tggtcgcccg caagttggtc gaggccaagg acgccgcgcc tggacaccac ggcagcgagg
      541 cacaggtccc ggccgccgag ctgcggcccg gcgatctggt ggtggtctcc gccggcgagc
      601 tgatcccggc cgatggcgag atcctgcatg gcctggccac catcaacgag tccgcggtca
      661 ccggcgaatc cgcgccggtc ctgcgcgagg ccggcaccga ccgcagcggg gtgatcggcg
      721 gtaccaaggt gctgtccgac gagatcgtgg tgcgcgtcac cgtggagccg gggcacagtt
      781 tcctggaccg catgatcgcg ctggtcgaag gcgccaaccg ccagaagacc ccgaacgaga
      841 tcgcgctgac catgctgctg gcggcgatga ccctgacctt cctgatcgtc tgcgcgacgc
      901 tgccggcgat cggtgccttc gtcggcgtga aggtcgatcc gctgttgctg atcgcattgc
      961 tggtctgcct gatcccgacc accatcggcg gcctgctgcc ggcgatcggc atcgccggga
     1021 tgaaccgcgc gctgtcggcg aacgtgctgg cgaaatccgg caaggcggtc gaagtcgccg
     1081 gcgacgtcga cgtcctcctg ctcgacaaga ccggcacgat cacccatggc gatcgccagg
     1141 ccaccgcctt ccatgcactg gccggcatca gcgccgacca gttgcgcgaa gccgcgctgc
     1201 tgtcatcgct ggccgacccc acgccggaag gcaagtcgat cgtgaaactg gcgcgcgaac
     1261 agggcacgcc gatgcaggac ccggaccagc tcgacgccgg cacgacgcag ttcatcccgt
     1321 tcactgcgca gacccgcatg tccggcgtgg acctcgacgg cggtgcgcgg ttgatccgca
     1381 agggcgccgg cgatgcgatc agccgccacg tgctgtcgct gggcggcgac gtgccggcgg
     1441 aactgaaggc acgcgtcgac caggtcgcgc gcaagggcgc caccccgctg gtggtgagcg
     1501 aaggccgctt cgcgctgggc gtggtcgagc tgtccgacgt ggtcaagcat ggcgtcaagg
     1561 aaaagttcgc gcggctgcgc gcgatgggca tcaagaccgt gatgatcacc ggcgacaacc
     1621 cactgaccgc ggccgccatc gccgccgagg ccggtgtcga cgactacatc gccgaagcca
     1681 cgccggagga caagctcgcg cggatccgca gcgaacaggc cggaggccgc ctggtcgcga
     1741 tggtcggcga cggcaccaac gatgcgcctg cgctggcgca ggccgacgtc gggttggcga
     1801 tgaactccgg cacgcaggcc gcgaaggaag ccggcaacat ggtcgacctc gacagcgatc
     1861 cggccaaatt gctggcggtg gtcgaagtcg gcaagcagca gctgatcacc cgcggcgcgc
     1921 tgaccacgtt ctcgctggcc aacgacgtct cgaagtattt cgcgatcctg ccggcgctgt
     1981 tcgccgcggc cattccctcg atggcgacgc tggacgtgat gggcctgtcc agcccgaaga
     2041 acgcggtgct cgcggcgctg atcttcaacg cgctggtcat cccggcgttg atcccgctgg
     2101 cgctgcgcgg cgtgcgtttc gccccggcca gcgccaccgt gctgctgcgc cggaacatgc
     2161 tgctgtacgg cggtggcggc gtcctgctgc ccttcatcgc catcaagctg atcgacctcg
     2221 cgctggccgc gacggttggc ccctgaatgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nccgatggag ctcgtcaccc gatccggcgg cgggctcgat ccgcacctca
     2401 cgccggccgg tgcgcgcgtg caggtggcgc gggtggcgaa ggcgcgtggc ctcgatgccg
     2461 gcatcgtcca gcaggccgtg gatgccgcca ccgaagcacc gcagttcggc gtgctgggcc
     2521 agccacgcat caacgtgctg ctgctgaacc tggcgctgga tgcgcggcca cggtgagcgg
     2581 gcgcacaatt cgcccatgaa tgcggatcgc agcgaggtcg tcaggcaggc gcaggccgat
     2641 gcgctgcttg gcgagctgca acgccagggc ggcgggcggc tgaccatctt cctcggcgct
     2701 gcacccggtg tgggcaagac ctacgcgatg ctgtcgcgcg ggcgcgaact gaagaagcaa
     2761 ggcaccgacg tggtcgtcgg cctggtcgag acgcacggcc gcgtggagac gcaggcgctg
     2821 ctggacggac tcgacgtgct gccgcgccgg cgcgtcgagc atcagggccg cacgctggag
     2881 gaacttgatc tcgacgccct gctcgcccgc aagccgcggg tcgcgctggt ggacgaactg
     2941 gcgcatcgca acgtccccgg cagccgccac gagcgccgtt ggcaggacgt ggtggaactg
     3001 ctggatgccg gcatcgacgt ctacaccacg gtcaacatcc agcacctgga aagcctcaac
     3061 gacatcatcc accggatcac cggcgtgcgc gtcagcgaga ccgtgccgga tctcgtcttc
     3121 gaccgcctgc gcgacatcaa gctgatcgac ctgccgccgc gcgagctgat cgagcgcctg
     3181 cagcagggca aggtctacat gccggaccag gcggcgcatg cgctgcagtc gttcttctcg
     3241 ccgtccaacc tggccgcgct gcgcgaactg gcgatgcaga ccgctgccga tcgcgtcgac
     3301 agcgacctgc gcgaagcgca gaccgcgcgt gggctgcccg gcagctcgtt gcgccgacgc
     3361 gtgctggtgg cgatcgatgg ccgcggccag tccgattacc tggtgagggt ggcgcggcgc
     3421 gtggccgagc ggcgcgatgc gccgtggagc gtggtcagcg tgcaatccgg cgcccacctc
     3481 gacgacgctg cgcaacgcga gctcgaccgc gcgttcgcgc tggcgcgccg gctcgggggc
     3541 gacgccgaac tgctgcacgg gccgagcatc gtcgatgcgc tgatcgacca tgccggccgc
     3601 agcggcgtct cgacgatcgt gatcgggcgc acccgcgagc gtccgttcgc gcgcatgttc
     3661 aaccgcaccc tgacccagca actgctgcag cgcgccgcgc acctggaact gaccatcgtc
     3721 agtacgccgg aggcgcgggc gaagtcgcgg cgctcgttgc acgacgtcgg cgccctgctc
     3781 acgcgcaccg atgcgggact ggcgttcgcc gccgcctcgc tggccacctt caccgcctgg
     3841 ctcgctgagc gcttcatcag cctcgacgac ctgtcgatgg tgttcatcgt cgcagtggtg
     3901 ctggtcgcct cgcggacgcg catgacggcg gcggtactgg ccgccggcct gtgcttcctg
     3961 tcctacaact tcttcttcat cgaaccacgc ctgaccctgt tcatcggcgc gcgacagggc
     4021 gtcgccacgg tgttcctgtt ccttgccgcc gcactggtcg cgggccggct ggcctcgcgc
     4081 ctgcgcatgc aggtggtcgc cctgcgcgca gccaatgcgc atgccacggc attgcaaggc
     4141 ttgagccggc aactggccgc ggccgccgat gccggcgagg tggccgaagc aggccggcgc
     4201 gcgttgaagc aggcgttagg agccgacgcg atcgtgcagg ccggcgacgt cgtggcggaa
     4261 acgcccggcc aggcgctgct gtccgacaag gatcgcgctg ccgccgactg ggcgctcaag
     4321 catcgccaac cggcaggtcg tttcaccgac accctgggca atgccgaatg gtggttcctg
     4381 ccgcttgccg gcgacaaggc cgccaccggc ctgctcggct tgcgcttccc cgctggcgcc
     4441 agccgcttcg gcagcgaaca acgcaggttg gtggaggcga tggcggacga catcgcgcag
     4501 gcgatgctgc gcacgcggtt ggtctcggac ctggaggacg cgcgcgtcac cggcgaaacc
     4561 gaacgcctgc gctctgccct gctgtcgtcg gtctcgcatg acctgcgctc gccgctggcg
     4621 gcgatcgtcg gcgccgcgga aaccctggac acctacggcc gcggcatggg cgaggacgac
     4681 cgccacagcc tgctcgacac cgtgcgcctg gagggcgagc ggcttgatcg ctacatccag
     4741 aacctgctcg acatgacccg cctcggccac ggcggtctca ccctcaatcg cgactggatc
     4801 ggcgtcgacg agctgatcgg ctcggcggcg acacgcttgc agcgttatca gcccgatgtg
     4861 cgcttcgaca tcgccctggc caagggactg gcgccactgt gggtgcatcc cgcgttggtc
     4921 gagcaggcga tcttcaacgt gatcgagaac gccgcgaagt tctcgcccgc gggcgaggcc
     4981 atcgcgatcg acgcccgcga catcgacggc gtgctgcgca tcgacgtcgc cgaccgtggc
     5041 cccggcatcc ccgaggatga acgcaagcgc atcttcgaca tgttcttcag cgtggagcgc
     5101 ggcgatcgcg gccgccaggg caccggactg gggcttgcga tcacccaggg catgatcggt
     5161 gcgcatggcg gcggcgtgga agcactgccg ggccccgatg gccgtggcac cacgatccgg
     5221 attaccctgc cacggatgac gcccgcagcc gccagcgcat gagcgagacc gccgcatcca
     5281 cggccgcgcg catcctcgtc gtcgacgacg agccgcagat ccgcaagttc ctggacatca
     5341 gcctgcgcgc gcagggccat cgggtggaaa ccgcggcgga cggcgaggcc ggcctgcagg
     5401 ccatggcgac gcatggcgcc gacctggtga tcctcgacct tggcctgccc gacctcgacg
     5461 gccatgaggt gctgtcgcgc ctgcgcgagt ggtcgcaggt gccggtgatc gtgctgaccg
     5521 tgcgcgccga cgaacgcgag aaagtcgccg cgctcgatgc cggcgccaac gactacgtga
     5581 ccaagccgtt cggcgtgcag gaactgatgg cgcgggtgcg cgccctgctg cgttcgcatg
     5641 ccaccgccgg cgaagcgccg ccggtgttcg acgatggacg gctgcgggtg gacctggcgc
     5701 ggcgcgaagt cttcctgctc ggcgaacccg tgcaactggc gcgcaaggaa tacgcgctgc
     5761 tcgcgctgct gctgcagcac gccgggcgcg tggtcaccca gccgcagatg ctgcgcgagc
     5821 tatgggggcg ccaccacgaa cacgacacgc attacctgcg cgtgctggtc ggcaagctgc
     5881 ggcagaagct gggcgacgat gccgccgcgc cgcgctggat cgccaccgaa ccgggcgtcg
     5941 gcctgcgctt catcggcgcg ccgcgctagt cgccaagccg gatcagtccc ggtcaatccc
     6001 gatccggcgc gcccagcggg aacaggtggc ggaacggccg ggcttcgtcc accgcacgcg
     6061 cgaccgaagg acgcgcaagc agtcgcgcgc gataggcgcg cagcactggg tacgcgtcgc
     6121 cgatcggatg ggtccagtcc gcatagaaca gcgacggcgc ggcggcgcag tcggccatcg
     6181 tgaagtcctc gccgcaggcc caggcgcgac cggcgagttc gccttccaac cagccgtagg
     6241 ccatctccag cttgtcggca gcgaacgcgc gcgcgtcgtc gcgcttgatc gcatcgccgg
     6301 tcagcgcgcc gccgacggcc gcctgcaccg ggctcatcac gtgcaggtcg aagcagcgat
     6361 ccatgaaacg cacgtcgagc gcggatatct catcttccgg cagcaggcgc acggcaccag
     6421 gatgggcgag ttgcaggtat tcgatgatga tgctggtctc cacgacctgc cgttcgccat
     6481 cgagcagcag cgggaacttg cgcagcggcc agcgccgcaa ccattcgccc gcgtgttccg
     6541 gatgg
//
LOCUS       DAOOCX010000120         6463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11218_length_6463_cov_5.872503, whole genome shotgun sequence.
ACCESSION   DAOOCX010000120 DAOOCX010000000
VERSION     DAOOCX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11218_length_6463_cov_5.872503"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1022
                     /locus_tag="PK399_09980"
     CDS             18..1022
                     /locus_tag="PK399_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HPW13487.1"
                     /translation="MAALDGIDLDIASGELVALLGPSGSGKTTLLRVIGGLLHADQGR
                     VLFDDIDATRLSLRERNVGFVFQHYALFRHMTVAENIAFGLRSRPRARRPDKATINKR
                     VQELLQLVQLPEHGGRYPDQLSGGQKQRIALARALAIDPSVLLLDEPFGALDAKVRVE
                     LRRWLRKLHERTGQTTLFVTHDQDEALELADRVVVMRAGKVEQVGTPDQIYHAPASPY
                     VFEFIGRANVLEGRVEQGRFQSGALQLDAAGTQDGPARLYVRPHDLVLSEPGAGALPA
                     RVRDVLRLGGRVTSELEIGGHARLLEQETFDAGAAPALSVGESVHLRPARYRVFAHAE
                     "
     gene            complement(1220..2122)
                     /locus_tag="PK399_09985"
     CDS             complement(1220..2122)
                     /locus_tag="PK399_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPW13488.1"
                     /translation="MDRLEAMTVFVAAVDGGSLSAAGRQLRMPLATVSRKVSDLEAHL
                     RARLLHRSTRKLTLTDAGRDYYASCKRILEDVGDAERDASGEYAEPRGELVIAAPIVF
                     GRVHVLPVIAQFLAAYPEVDVRLVQGDRNANLLEDHLDLAVRIGALPDSSMTATAVGS
                     VRRVVCASPAYLAARGTPQSPDELAGHCCVSFDALGAGNAWRFETGGGYRDVAVRSRL
                     VVNTADAAIDAAIGSAGITRLLSYQVAQALQEGRLQRLLPEFESTPAPVNLLFDGRRR
                     LPLKLRAFIDFATPRLRERLANIA"
     gene            2246..2797
                     /locus_tag="PK399_09990"
     CDS             2246..2797
                     /locus_tag="PK399_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711655.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HPW13489.1"
                     /translation="MSRIAIPASIAAAPAASQPLLEAVNKQIGSVPNLFRLVANSPAA
                     LEGYLGMNGALAKGQLPAATRERIALAVAEINGCNYCLSAHTYLGKNLAKLDAAEIAA
                     NRSGASNDIKADAAVRFAAKVTQQRGKVSSEDVAAVKAAGYSEAQVIEIVQHVALNTW
                     TNYINEVAGTEIDFPVVQARLAA"
     gene            2864..3328
                     /locus_tag="PK399_09995"
     CDS             2864..3328
                     /locus_tag="PK399_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW13490.1"
                     /translation="MNRPPLPPFNLEAAKQKVRMAEDGWNSRDPARVALAYTEDSRWR
                     NRSEFLQGRAAIEAFLARKWAQEREYRLVKELWAFQGNRIAVRFAYESQDASGAWTRS
                     YGNENWEFDEHGLMRLRIASINDLSIDEHQRLLRWPLGPRPAGHASLSELGL"
     gene            3333..3932
                     /locus_tag="PK399_10000"
     CDS             3333..3932
                     /locus_tag="PK399_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPW13491.1"
                     /translation="MARVASDIAFTPIVKSLQARKGSRRAYAHMEANGSWRTDITPEL
                     EAFIAAQRSVFLATVNGEGQPYIQHRGGPPGFLRALDPRTLAFVDFSGNRQYITSGNL
                     LENPKAHLFLIDYRQRKRVKIWGEARMVEADDAWLSRLMPEGYKARPEQVLLFNVAAW
                     DANCPQHIPLRLEAADVQAALQEKDRRIEELEAELARAR"
     gene            complement(4054..4299)
                     /locus_tag="PK399_10005"
     CDS             complement(4054..4299)
                     /locus_tag="PK399_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003851381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HPW13492.1"
                     /translation="MFGGILGYIFGGLVIGILARLVKPGADPMGWILTIVLGIAGAVI
                     GGWAAGNFGLGRMLTWVVAIAAAIVLLFVYEAMRKKK"
     gene            complement(4364..5869)
                     /locus_tag="PK399_10010"
     CDS             complement(4364..5869)
                     /locus_tag="PK399_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPW13493.1"
                     /translation="MTTNHFDPSQARDFVGAKWDAEIVPQLVEYIRIPNKSPMFDADW
                     VKHGYMEQAVSLMETWARAQDVPGMTLEVVRLEGRTPLIFIEVPAANGGRNDDCILLY
                     GHLDKQPEMTGWDEGKGAWEPVMEGDRLYGRGGADDGYAIYGSLAAVMALQAQGAAHA
                     RCVVLIEACEESGSYDLPAYVDHLAERIGKPSLVVCLDSGCGNYDQLWCTTSLRGMAG
                     GNLTVKVLEEGVHSGDASGIVPSSFRIIRELLSRIEDERTGRILVDGMHVEIPAERRQ
                     QAGKVAEVLGTEVYDKFPFLPGMTPMASDLTELVLNRTWRPALSITGADGLPALSSAG
                     NVLRPQTSVKLSLRLPPTLDGKRGGELLQEALLKDPPYGAQVSLHLEKASTGWNAPAL
                     APWLENAIDQASQDFFGKPAMYMGEGGTIPFMGMLGEKFPGAQFMITGVLGPHSNAHG
                     PNEFLHIPMGKGVTACVARVIAEHNAASLRGETTGVAAVAGGQVHGDHGCC"
     gene            6082..6435
                     /locus_tag="PK399_10015"
     CDS             6082..6435
                     /locus_tag="PK399_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005919170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HPW13494.1"
                     /translation="MKFLTTLFSSLLLSLALAGAAIAGESVNINTADAATIDRVLLNV
                     GPAKAQAIVDYRKANGAFRSPEQLALVKGIGLKTVEKNRDRIVVGAARPAAARPGATV
                     AKAQPRPAPKPVARR"
BASE COUNT         1096 a         2212 c         2142 g         1013 t
ORIGIN      
        1 ccaagcacta tcccggcgtg gccgcgctgg acggcatcga cctggacatc gcctcgggcg
       61 aattggtcgc gttgctcggc ccgtctggtt ccggcaagac cacgctgctg cgggtgatcg
      121 gcggcctgct gcatgccgac caggggcgcg tgctgttcga cgatatcgat gcgacgcggc
      181 tcagcctgcg cgagcgcaac gttggcttcg tcttccagca ttacgcgctc ttccggcaca
      241 tgacggtggc ggaaaacatc gccttcggcc tgcgcagccg gccgcgcgcg cgccgtcccg
      301 acaaggcgac catcaacaag cgcgtgcagg agctgctgca actggtgcaa ctgccggaac
      361 acggcggccg ctatccggat caactctccg gcggtcagaa gcagcgcatc gcactggcgc
      421 gtgcgctcgc catcgatccc agcgtgctgc tgctcgacga gccgttcggc gcgctcgacg
      481 cgaaggtccg ggtcgagttg cgccgctggc tgcgcaagct gcacgaacgt accggccaga
      541 ccacgctgtt cgtcacccat gaccaggacg aagcgctgga actggcggat cgcgtggtcg
      601 tgatgcgcgc ggggaaggtc gagcaagtcg gcacgcccga ccagatctac cacgccccgg
      661 catcgcccta cgtgttcgag ttcatcggtc gcgccaacgt gctggaaggt cgcgtcgagc
      721 aggggcgctt ccagtcgggt gccctgcaac tggatgcggc cggcacgcag gacgggccgg
      781 cgcgcctgta tgtgcgaccg cacgacctgg tcctgtccga acccggcgca ggtgcattgc
      841 cggcgcgggt gcgcgatgtg ctgcgcctgg gcgggcgggt gaccagcgaa ctcgagatcg
      901 gcggccacgc tcggctgctc gaacaggaaa ccttcgatgc cggcgcggca ccggcgttgt
      961 cggtcggcga gtccgtgcac cttcggccgg cgcgctatcg ggtcttcgcg cacgcggagt
     1021 gaccgatgcg acatcgccta gaaaaagccc cgcacatggc ggggcctttt cctgtcggga
     1081 cattggctca tccgggggcg gcttcggccg cgagtgatgg cgacagacga ctgcaggcga
     1141 acgcacatgt cggcggcgcg atgcggcgga atcagttgga taggtacctg atgcgcgtgc
     1201 atcgtggtcg cgcatcggct cacgcgatat tggcaaggcg ctcgcgtaat cgcggggtgg
     1261 cgaagtcgat gaatgcgcgc aacttcagcg gcagcctgcg gcgaccgtcg aacaggaggt
     1321 tgaccggcgc tggcgtgctt tcgaactccg gcagcagccg ctgcaagcgc ccttcctgca
     1381 gggcttgggc gacctggtag gacagcaggc gggtgatgcc ggcgctgccg atggcggcgt
     1441 cgatggccgc gtccgcggtg ttgacgacca ggcgcgaacg caccgcgaca tcgcggtatc
     1501 cgccgccggt ttcgaagcgc caggcattgc cggcgcccag cgcatcaaag gacacgcagc
     1561 agtgtccggc gagttcgtcg ggcgactgcg gcgtgccgcg cgccgccagg taggccgggc
     1621 ttgcgcatac cacgcgtcgc acgctgccaa ccgcggtggc ggtcatgctg ctgtcgggca
     1681 gggcaccgat gcgcacggcg aggtccaggt ggtcctcgag caggttggcg ttgcggtcgc
     1741 cctgcaccag cctcacgtcc acttccggat acgcggccag gaattgcgca atcaccggca
     1801 gcacgtgcac gcgcccgaac acgattggcg cggcaatcac cagttcgccg cggggctcgg
     1861 cgtattcgcc gctggcgtcg cgctcggcat cgccgacatc ttcgaggatg cgcttgcagg
     1921 acgcgtagta atcgcggccc gcatcggtga gcgtgagctt gcgggtagag cgatgcagca
     1981 ggcgcgcgcg caggtgcgct tccaggtcgg acaccttgcg actcaccgtg gccaggggca
     2041 tgcgcaattg ccgccccgcc gcggacaggc tgccgccatc cacggcagcg acgaatacgg
     2101 tcatcgcttc gaggcgatcc acggaatatt ccaaaatatg gaaggaagat tccaatgtac
     2161 acggactaat gcgtgaatgt ggaagtatct aagctttctc caccgggcaa accgtccgcc
     2221 agccacccac ccaaggagaa accccatgag ccgtatcgcc atccccgcca gcatcgccgc
     2281 cgcccccgct gcttcccagc ccttgctgga ggcggtcaac aaacagatcg gcagcgtgcc
     2341 gaacctgttc cgcctggtcg ccaacagccc ggccgccctc gagggctacc tgggcatgaa
     2401 cggcgccctc gccaagggcc agctgcccgc cgccacccgc gagcgcatcg ccttggccgt
     2461 ggccgagatc aacggttgca actactgcct gtcggcgcat acctacctgg gcaagaacct
     2521 ggccaagctc gacgccgccg agatcgccgc caaccgcagt ggcgcctcga acgacatcaa
     2581 ggccgacgcc gccgtgcgtt tcgccgccaa ggtcacgcag cagcggggca aggtcagcag
     2641 cgaagacgtc gccgcggtga aggctgccgg ttacagcgag gcgcaggtca tcgagatcgt
     2701 gcagcacgtc gccctgaaca cctggaccaa ctacatcaac gaagtcgccg gcaccgagat
     2761 cgacttcccg gtggtgcagg cgcgcctggc cgcgtaacgc aacgcgacaa gaccgccgcg
     2821 ccagcgggtg cggcggttgt tttccgacaa tcgaggagga accatgaacc gtccaccgct
     2881 gccgcctttc aacctggaag ccgccaagca gaaagtacgg atggccgaag acggctggaa
     2941 ctcgcgcgac cccgcgcgcg tcgccctggc ttacaccgag gacagccgct ggcgtaaccg
     3001 cagcgagttc ctgcaaggcc gcgctgcgat cgaagcgttc ctcgcgcgaa agtgggcgca
     3061 ggagcgcgaa tatcgcctgg tgaaggaact ctgggcattc cagggcaacc gcatcgcggt
     3121 gcgcttcgcc tacgaatcgc aggatgccag cggcgcctgg acgcgcagct acggcaatga
     3181 aaattgggaa ttcgacgaac acgggctgat gcgcctgcgc atcgcgagca tcaatgacct
     3241 gtccatcgac gagcaccagc gcctgctgcg ctggcccctg ggcccgcgcc cagccggcca
     3301 tgcctcgttg agcgagctcg ggctatagcg gcatggcccg cgtcgccagc gatatcgcct
     3361 tcaccccgat cgtgaaatcc ctgcaggcgc gcaagggttc gcgtcgggcc tacgcgcaca
     3421 tggaagccaa tggctcctgg cggaccgaca tcacgccgga actggaggcg ttcatcgccg
     3481 cccaacgcag cgtcttcctg gcgaccgtga atggcgaagg gcagccgtat atccagcatc
     3541 gcggagggcc gccggggttc ctgcgcgcgc tggatccgcg cacgctggcc ttcgtggatt
     3601 tttccggcaa tcgccaatac atcaccagcg gcaatttgct ggagaacccg aaggcgcact
     3661 tgttcctcat cgattaccgg caacgcaagc gcgtgaagat atggggcgaa gcgcgcatgg
     3721 tcgaggccga cgacgcctgg ctttcgcggc tgatgcccga aggctacaag gcccggccgg
     3781 agcaggtgct gttattcaat gttgcggcgt gggacgccaa ttgcccccag cacatcccgc
     3841 tgcgcctcga ggcggccgac gtgcaggcgg cgctgcagga aaaggatcgt cgcatcgagg
     3901 aactggaagc ggaactcgcg cgcgcgcgct gaagcgcaaa cgactgctgt ctgctccgcg
     3961 ctcacgatga cggcgcgcgc agtgaccgag gcaacatcgg acaaaaaagc cccgcacaca
     4021 gcggggcttt ttttgcggtt ggaacaagcg tgctcacttc ttcttgcgca tcgcctcgta
     4081 cacgaacagc aggacgatgg cggcggcgat cgcgaccacc caggtcagca tccggcccag
     4141 gccgaagttg ccggcggccc agccgccgat cactgcgccg gcgatgccga gcacgatggt
     4201 gaggatccag cccatcggat ccgcaccggg cttgaccagc cgggccagga tgccgatgac
     4261 gaggccgccg aagatgtaac cgaggattcc accgaacatg ttgcctcccg atccgttgtt
     4321 ggaatgacgc gggggatcct agcagccggg tgtgcaggaa ccgtcagcag caaccgtggt
     4381 cgccgtgcac ctgcccaccc gccaccgcgg ccacgcccgt ggtctcgccg cgcaggctgg
     4441 ccgcgttgtg ttcggcgatc acccgcgcca cgcaggcagt cacgcccttg cccatcggga
     4501 tgtgcaggaa ttcgttcggg ccgtgcgcgt tggaatgcgg gcccagcacg ccggtgatca
     4561 tgaactgcgc gccggggaat ttctcgccga gcatgcccat gaacgggatg gtgccgcctt
     4621 cgcccatgta catcgccggc ttgccgaaga agtcctggct ggcctggtcg atcgcattct
     4681 ccagccacgg cgccagtgcc ggtgcattcc agccggtgct cgccttctcc aggtgcaggc
     4741 tgacctgtgc cccatacggc ggatccttga gcagtgcctc ctgcagcagt tcgccgccgc
     4801 gcttgccgtc cagcgtgggc ggcaggcgca ggctcagctt caccgaggtc tgcggacgca
     4861 gcacgttgcc ggccgacgac aatgcgggca ggccatcggc gccggtgatc gacagcgcgg
     4921 gtcgccacgt gcggttgagc accagttcgg tcaggtcgct ggccatcggc gtcatgccgg
     4981 gcaggaacgg gaacttgtcg tagacctcgg tgcccaggac ctcggcgacc ttgccggcct
     5041 gttgccggcg ttccgcggga atctcgacgt gcatgccgtc gacgaggatg cggccggtcc
     5101 tttcgtcctc gatccgcgac agcagctcac ggatgatgcg gaagctggac ggcacgatgc
     5161 cggaggcgtc gccggagtgc acgccttcct cgagcacctt caccgtcagg ttgccgccgg
     5221 ccatgccgcg cagcgaggtg gtgcaccaca gctggtcgta gttgccgcag cccgaatcca
     5281 ggcagaccac cagcgagggc ttgccgatgc gctcggccag gtggtcgaca taggcgggca
     5341 ggtcgtagct gccggattcc tcgcaggcct cgatcagcac cacgcagcgc gcatgcgcag
     5401 cgccttgtgc ctgcaacgcc atcaccgcgg ccagcgaacc gtagatcgcg tagccatcgt
     5461 cggcgccacc gcggccgtac aggcggtcgc cttccatcac cggttcccaa gcgcccttgc
     5521 cttcgtccca gccggtcatc tccggctgct tgtccaggtg gccgtagagc aggatgcagt
     5581 cgtcgttgcg accgccattc gctgccggca cctcgatgaa gatcagcggc gtgcggcctt
     5641 ccaggcgcac cacttccaat gtcatgccag gcacgtcctg cgcgcgcgcc caggtctcca
     5701 tcagcgacac cgcctgctcc atgtacccgt gcttcaccca gtcggcgtcg aacatcggcg
     5761 acttgttggg gatgcggatg tactccacca gctgcgggac gatctcggcg tcccatttgg
     5821 cgccgacgaa gtcgcgggcc tggctggggt cgaagtggtt ggtggtcatg cgtgtcgatc
     5881 cgaagcgagt gcgaggccgc cattctactc ccgacccatt tccgcccttc gcgggtgggc
     5941 ggtttcctac cgccatccgg gcgaagcgcc ggtccggagg cggggaggtc gccgatcgag
     6001 cgggtaacgc ggcatcggca acctgtcttc accggatcgc agcctgcggt cgcggttcga
     6061 acccaccccg aggagaccgc catgaagttc ctgaccaccc tgttctcgtc cctgctgctg
     6121 tcgctggccc tggctggtgc tgccatcgcc ggcgagagcg tcaacatcaa taccgccgac
     6181 gccgccacca tcgaccgcgt cctgctgaac gtcggcccgg ccaaggcgca ggccatcgtc
     6241 gattaccgca aggccaacgg cgcgttccgc agccccgagc aactggccct ggtcaagggc
     6301 atcggcctga agacggtaga gaagaaccgc gaccgcatcg tggtcggggc cgcccgtccc
     6361 gccgcggccc gcccgggtgc caccgtggcc aaggcccagc cgcgccccgc accgaagccg
     6421 gtcgcccgtc gctgatccca cgcaggcggc gatacactgc ggc
//
LOCUS       DAOOCX010000121         6395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11357_length_6395_cov_8.091640, whole genome shotgun sequence.
ACCESSION   DAOOCX010000121 DAOOCX010000000
VERSION     DAOOCX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11357_length_6395_cov_8.091640"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /gene="aceA"
                     /locus_tag="PK399_10020"
     CDS             complement(<1..980)
                     /gene="aceA"
                     /locus_tag="PK399_10020"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HPW13495.1"
                     /translation="MKNHLPTAEQLKLDWANNPRWAGITRAYSAEDVVRLRGTVHVEH
                     SIARLGAEKLWKSLHTEDFVNALGAMTGNQAMQQVKAGLKAIYLSGWQVAADANLAGE
                     MYPDQSLYPANSVPQVVKRINNTLLRADQLHHAEGDHSIDFLQPIVADAEAGFGGVLN
                     AYELMKAMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVEKLTAARLAADVMGV
                     PTIIVARTDAEAADLVTSDIDDNDKPFCTGERTIEGFFKTDNGLDQAISRGLAYAPYA
                     DLVWCETGKPDLEFARKFAEAIHAKFPGKLLAYNCSPSFNWKKNLDDATIA"
     gene            complement(1039..2661)
                     /gene="aceB"
                     /locus_tag="PK399_10025"
     CDS             complement(1039..2661)
                     /gene="aceB"
                     /locus_tag="PK399_10025"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HPW13496.1"
                     /translation="MSAVLQDRPAPGAVDLTRNLPGQEALLTPAAQTFLAGLHVRFEG
                     ERQARLAARRARQAAFDAGALPDFRADTASIRAADWRVAPIPAALQDRRVEITGPVDP
                     KMVINALNSGANCYMADFEDSTSPTWANLLAGQQALREAIAGTLAFSGDNGKQYALRP
                     ESEQAVLIVRPRGWHLDEKHVLVDGQRLSASLFDLGLFCFHNAAALAAKDRGPYFYLP
                     KLQSMEEAALWNAVLGDIETTLGLPHGQLKVTVLIETLPAAFEMDEILYALKDRIVGL
                     NCGRWDYIFSYIKTLRRHRDRVLPERGQVTMVQPFLRAYSELLVKTCHRRGAHAMGGM
                     AAQIPISGDDAANGAALARVRADKLREVTAGHDGTWVAHPALIPLAREVFDAHMPEAN
                     QVHIARDDVHAERDDLLRPSLGTITKAGFEGNVEVCVRYLAAWLDGNGCVPIHWLMED
                     AATAEIARTQLWQWLHFADDGHEPLHLDDGTPVDFALLERALIGLPSKFVDQLRMPGA
                     SRINEAIGMLDRLTHADELADFLTLPAYERID"
     gene            2801..3745
                     /locus_tag="PK399_10030"
     CDS             2801..3745
                     /locus_tag="PK399_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPW13497.1"
                     /translation="MRFAYKGSRLKPLRAFCQVARLGSVSRAAEALYLSQPAVTLQLQ
                     ALERELGVRLLERSGRRLTPTREGELLYELAKPLVEGLDGLAASFREQVRGLDAGELN
                     VAAGSSTILYLLPGIVDAFRQRHADVRLSLHNVTGASGLDLLRNDAVDLAVGSMLDVP
                     ADLTYAPVYSFEPMLIMPPDHPLASKRDLTLQDLSPYGLILPPKRLTTYRLVDLVFQQ
                     NRVPYTVALEVGGWEVIKQYVAMGLGISIVTAICLTEADRQRLAARSLARWFPSRSYG
                     IVMRKGKFLSSQARAFVELIKPELLSPREQDRGGHSER"
     assembly_gap    3813..3912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3952..4959)
                     /locus_tag="PK399_10035"
     CDS             complement(3952..4959)
                     /locus_tag="PK399_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HPW13498.1"
                     /translation="MAQRHYAVRAGVLVALDQPLLVLHGGAGVEPGDLTPAEEDAARA
                     ALRDALKAGYAKLQAGASSVDAVTAAITVLEDAPQFNAGRGAVFTHDGRNELDTSLMD
                     GASGKAGAAAGLHRVKNPILLARAIMDKSKHVMMVGDGAETFAKEQGIALVDPAYFRV
                     EKRWQQLQKALDEEKKAQASNTPLELPGKAYFGTVGALALDAQGRLAGGTSTGGMTNK
                     RYGRVGDSPIIGAGTWADDRCAVSGTGWGEFYIRSAAAHEICARVRLAGDPLARAADR
                     VINRDIPKAGGDGGAIALGADGSVAFPFNTGGMYRGWIGSDGVPHVAIYKTDALAVPG
                     K"
     gene            complement(5377..5703)
                     /locus_tag="PK399_10040"
                     /pseudo
     CDS             complement(5377..5703)
                     /locus_tag="PK399_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
     gene            5980..>6395
                     /locus_tag="PK399_10045"
     CDS             5980..>6395
                     /locus_tag="PK399_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW13499.1"
                     /translation="MQAELDRWFIDEILVHEDALVRYLQRCWSRRDEIHDLRQEVYAR
                     VYEAAARSLPTQPKSFLFASARHLMTDRLRRSRVVSIEPMGDFEPSHVLVDDVSPERW
                     CGGRQGLLRLAEAFDRLPDRCREVVWLRRVEDLSQK"
BASE COUNT          927 a         2136 c         2183 g         1049 t
ORIGIN      
        1 gcgatggtgg cgtcgtccag gttcttcttc cagttgaagc tgggcgagca gttgtaggcc
       61 agcagcttgc ccgggaactt cgcgtggatc gcctcggcga acttgcgggc gaattccagg
      121 tccggcttgc cggtctcgca ccagaccagg tcggcgtacg gcgcgtaggc caggccacgg
      181 ctgatcgcct ggtccaggcc gttgtcggtc ttgaagaagc cttcgatcgt gcgctcgccg
      241 gtgcagaacg gcttgtcgtt gtcgtcgatg tcggaggtca ccaggtcggc ggcctcggcg
      301 tcggtgcggg ccacgatgat cgtcggcacg cccatcacgt ccgcggccag gcgcgcggcg
      361 gtcagcttct cgacggcctc gcgcgtcggc accagcacct tgccacccat gtgcccgcac
      421 ttcttcaccg aggccagctg gtcctcgaag tgcacgccgg cggcgccggc ctcgatcatc
      481 gccttcatca attcataggc gttcagcacg ccgccgaaac cggcttcggc atcggccacg
      541 atcggctgca ggaaatcgat ggaatggtcg ccctcggcat ggtggagctg gtcggcgcgc
      601 agcagggtgt tgttgatgcg cttgaccacc tgcggcaccg aattggccgg gtacagcgac
      661 tggtcgggat acatctcgcc ggccaggttg gcgtcggcag cgacctgcca gccactcagg
      721 tagatcgcct tcaggccggc cttgacctgc tgcatcgcct ggttgccggt catggcgccg
      781 agcgcgttga cgaaatcctc ggtatgcagc gacttccaca gcttctccgc gccgagccgg
      841 gcgatcgaat gctcgacgtg cacggtgccg cgcaggcgca ccacgtcctc ggccgagtag
      901 gcgcgggtga tgccggccca acgcggattg ttggcccagt ccagcttcag ttgttcggcg
      961 gtcggcaggt ggttcttcat ggcgattcct cggtgggggt gcagcaagca atgagatggg
     1021 gtgtttggag gaagcaggtc agtcgatgcg ttcgtaagcg ggaagcgtga ggaagtcggc
     1081 gagttcatcc gcatgggtga gccggtcgag catcccgatc gcctcgttga tgcggctggc
     1141 gccgggcatg cgcagctggt cgacgaactt gctgggcagg ccgatgaggg cgcgctccag
     1201 cagggcgaaa tcgaccgggg tgccgtcgtc caggtgcagc ggctcgtggc cgtcgtcggc
     1261 gaagtgcagc cattgccaca gctgggtgcg ggcgatctcg gcggtggcgg cgtcttccat
     1321 cagccagtgg atgggcacgc agccgttgcc gtcgagccat gcggccaggt agcgcacgca
     1381 gacctcgaca ttgccctcga agccggcctt ggtgatcgtg cccagcgagg gccgcagcag
     1441 gtcgtcgcgc tccgcgtgca cgtcgtcgcg ggcgatgtgg acctggttgg cttccggcat
     1501 gtgcgcgtcg aacacctcgc gggccagcgg gatcagcgca ggatgggcga cccaggtgcc
     1561 gtcgtggccg gcggtgactt cgcgcagctt gtcggcgcga acgcgggcca gcgcggcacc
     1621 attggccgcg tcatcgccgc tgatcgggat ctgcgcggcc atgccgccca tcgcgtgcgc
     1681 gccgcggcga tggcaggtct tcaccagcaa ctcggagtag gcgcgcagga acggctgcac
     1741 catcgtcacc tggccgcgct cgggcagcac ccggtcgcgg tgccggcgca gggtcttgat
     1801 gtaggaaaag atgtagtccc agcggccgca gttgaggccg acgatgcggt ccttgagcgc
     1861 gtacaggatc tcgtccatct cgaacgccgc cggcagggtc tcgatcagca cggtgacttt
     1921 cagctggcca tgcggcaggc ccagcgtggt ttcgatgtcg cccagcaccg cgttccacag
     1981 cgccgcctct tccatcgact gcagcttggg gaggtagaag tacgggccgc gatccttcgc
     2041 cgccagcgcg gcggcgttgt ggaagcagaa caggcccagg tcgaacaggc tggccgacag
     2101 gcgctggcca tcgaccagca cgtgcttctc gtccaggtgc cagccgcgcg ggcgcacgat
     2161 cagcacggcc tgctcggact ccgggcgcag ggcgtactgc ttgccgttgt cgccgctgaa
     2221 ggccagcgtg ccggcgatcg cctcgcgcag cgcttgctgg ccggccagca ggttggccca
     2281 ggtcggcgac gtgctgtcct cgaagtcggc catgtagcag ttggcgccgg agttcagcgc
     2341 gttgatcacc atcttcgggt cgaccggccc ggtgatctcc acccggcggt cctgcagcgc
     2401 cgccgggatc ggggcgacgc gccaatcggc ggcgcggatc gatgcggtgt cggcgcggaa
     2461 atccggcagc gcgccggcat cgaaggcggc ctggcgggcg cggcgggcgg ccaggcgggc
     2521 ctggcgttcg ccctcgaagc ggacatgcag gccggccagg aaggtttgcg cggccggggt
     2581 gagcagcgcc tcctggccag gcaggttgcg ggtcaggtcc acggcgcccg gcgcggggcg
     2641 gtcttgcagc acggccgaca tcggcaactc cagcgttcga tcagtgggga atcgaaccat
     2701 ccgcctgcgt ttggtatttg acaaaccaga tttgtcaatg attagtattt gaaaaccaaa
     2761 taccgtggtc gacacgttgg ccgagagccc ctccagcact atccgcttcg cctacaaggg
     2821 ctcccggctc aagcccttgc gggcgttctg ccaggtcgcg cgactgggtt cggtgagccg
     2881 cgccgcggaa gcgctgtacc tgagccagcc ggcggtgacc ctgcaactgc aggcgctgga
     2941 acgcgaactg ggcgtgcgcc tgctggaacg cagcggccgc cggctgaccc cgacccgcga
     3001 gggcgagctg ctgtacgaac tggccaagcc cctggtggaa gggctggatg gtttggccgc
     3061 cagcttccgc gagcaggtcc gcgggctgga cgcgggcgaa ctcaacgtcg ccgccggcag
     3121 ctcgaccatc ctgtacctgc tgcccgggat cgtggatgcg ttccgccagc gccatgccga
     3181 cgtgcgcctg agcctgcaca acgtcaccgg cgccagcggg ctggacctgc tgcgcaacga
     3241 tgcggtggac ctggcggtgg gctcgatgct ggatgtgccg gcggacctga cctacgcgcc
     3301 ggtctacagc ttcgagccga tgctgatcat gccgcccgac catccgctgg catccaagcg
     3361 cgacctgacc ctgcaggacc tgtcgccgta cggcctgatc ctgccgccga agcgcctgac
     3421 cacctatcgg ctggtcgacc tggtgttcca gcagaaccgg gtcccctaca cggtggcgct
     3481 ggaggtcggc ggctgggagg tgatcaagca gtacgtggcg atggggctgg gcatcagcat
     3541 cgtcaccgcg atctgcctga ccgaagccga ccgccagcgc ctggccgcgc gctcgctggc
     3601 gcgctggttc ccctcgcgca gctacggcat cgtcatgcgc aagggcaagt tcctgtcatc
     3661 gcaggcgcgc gccttcgtcg agctgatcaa gcccgaactg ctgtccccgc gcgaacagga
     3721 tcgcggcggg cattcggagc gttgagcgaa aaggacaaaa aggcgggtca gcaaaaaggc
     3781 gtgaaaaagg cgggtcagag tcgagtttcg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nngctgaccc gcctttttga cccgcctttt ctgtgtttcg gtcacttgcc
     3961 gggcaccgcc agcgcgtcgg tcttgtagat cgccacgtgc ggcacgccgt cactgccgat
     4021 ccagccgcgg tacatgccgc cggtgttgaa cggaaacgcc acgctgccgt ccgcgccgag
     4081 cgcgatcgcg ccgccgtcgc caccggcctt ggggatgtcg cggttgatga cgcgatccgc
     4141 ggcacgggcg agcgggtcgc cggccaggcg cacgcgcgcg cagatctcgt gggcggcggc
     4201 gctgcggatg tagaactcgc cccagccagt gccagacacc gcgcagcggt cgtcggccca
     4261 ggtgccggcg ccgatgatcg gcgagtcgcc gacccggcca tagcgcttgt tggtcatgcc
     4321 gccggtcgag gtgccgccgg ccagccgtcc ctgcgcgtcc agggccagcg cgccgacggt
     4381 gccgaagtaa gccttgcccg gcagttccag cggcgtgttc gacgcctgcg ccttcttctc
     4441 ctcgtcgagc gccttctgca attgctgcca gcgcttttcg acgcggaaat acgccggatc
     4501 caccaacgcg atcccctgct ccttcgcgaa cgtttcggcg ccgtcgccga ccatcatcac
     4561 gtgcttcgat ttgtccatga tcgcgcgcgc cagcaggatc ggattcttca cccgatgcag
     4621 gcccgccgcc gcgccggcct tgccgctggc gccgtccatc agcgaggtgt ccagctcgtt
     4681 gcggccgtcg tgggtgaaca ccgcgccgcg accggcgttg aactgcgggg cgtcctccag
     4741 cacggtgatc gcggcggtca ccgcatcgac cgacgacgca cccgcctgca gcttcgcgta
     4801 accggccttg agcgcgtcgc gcagggcggc gcgcgcggcg tcctcttcgg ccggggtcag
     4861 gtcgccgggc tccacgccgg caccaccgtg gagtaccagc agcggctggt cgagcgcgac
     4921 cagcacgcca gcgcgcaccg cgtaatggcg ctgcgccagc ggcttctcga cgcggccgtc
     4981 gggcaggtgc agcaccgagt ccacgccggc gaccacggtg tcgacgcggt cgaggttcat
     5041 cgccgagtct tcttcctgct tctgggcgaa gccacccttg accacgatcg cgccggcacc
     5101 cggcgcggtg gcatagacgc gcgcgcgtcc atccgcctcg accgcgaccg cggcgtcctc
     5161 gtccacgccg aggccgatca acggcttgcc ggcgatgtgc tcgcccttgg cgacgaaggc
     5221 gatcagccga cccagccgcg cgcgctcgct gaagtgggtg tcggtgatca cgcccttgag
     5281 ctgggcgatg tgcaggaagt cggtttcgat ggtgttgccg tcgccgagcg gatcggccag
     5341 cgcagccggg gtggcctggc tgccgccgtc catcgcgcca tacaggtact cgccctgcat
     5401 cgccaggccg gcactggtgc caccgagcgg cttgcccgct cgcacgtgcg cgtccagtgc
     5461 ctcggcgatc gcggtcccgc gccagaagcg cacgtagcgc gactggtcgc caccggccag
     5521 gaagatgccg tccgcgcgct tcagcgcggc caggatcttc gggtcggtgg acgcctcgcg
     5581 atcattgaac acgaaggtct cgaccgattt gatgccaccg acctcgttga agaattcctc
     5641 gccgatctcg ccggcctgcg acgcccgcaa caccacgatg tggccgttgc cggcctgctt
     5701 catgaaccag cgcatggcat cgaagttgcg gtcgccgccg cccatcagca gcagcccggg
     5761 ctggaccttg cccggcgtct tcgccgccag gtcgccgatc acgtagtggc cgacatcggc
     5821 cgccagcgcg ccgccagcca acaacaggcc aagcgctgca acgaggaatt tcgaaacgcc
     5881 ggcacgtcgc atcatcgtgt gtccctgttc gccgtcatcg gccgccacat tggaagacga
     5941 acggcgacgc ggtttccccc gttcggctat ggtgccgcga tgcaggccga gctcgaccgc
     6001 tggttcatcg acgaaatcct cgtccacgag gatgcgctgg tgcgctacct gcagcgctgc
     6061 tggtcgcgcc gcgacgagat ccacgacctg cgccaggaag tctacgcacg tgtctacgag
     6121 gccgccgcca ggtcgctgcc gacccagccg aagtcgttcc tgttcgccag cgcgcggcac
     6181 ctcatgaccg atcggttgcg gcgcagccgg gtggtctcga tcgagccgat gggtgatttc
     6241 gagccctcgc acgtcttggt ggatgacgtg tcgccggagc gctggtgcgg cggcaggcag
     6301 ggattgctgc ggctggccga ggccttcgac cgcctgcccg accgctgccg cgaggtggtc
     6361 tggctgcgcc gcgtcgagga cctgtcgcag aagga
//
LOCUS       DAOOCX010000122         6382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11382_length_6382_cov_9.091671, whole genome shotgun sequence.
ACCESSION   DAOOCX010000122 DAOOCX010000000
VERSION     DAOOCX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11382_length_6382_cov_9.091671"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /gene="groL"
                     /locus_tag="PK399_10050"
     CDS             complement(<1..1365)
                     /gene="groL"
                     /locus_tag="PK399_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411226.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPW13500.1"
                     /translation="MAAKDIRFGEDARAKMVRGVNVLANAVKATLGPKGRNVVLQKSF
                     GAPTITKDGVSVAKEIELADAFENMGAQMVKEVASKTSDNAGDGTTTATVLAQAFIRE
                     GMKAVAAGMNPMDLKRGIDQAVKAAVGELKSLSKPSSTSKEIAQVGAISANSDANIGD
                     LIAQAMDKVGKEGVITVEEGSGLDNELDVVEGMQFDRGYLSPYFINNQQSMSAEMDDP
                     FILLHDKKISNVRDLLPVLEGVAKAGKPLLIVAEEVEGEALATLVVNTIRGIVKVVAV
                     KAPGFGDRRKAMLEDMATLTGGTVISEEVGLSLEKATIKDLGRAKKIQVTKENTTIID
                     GAGDADGIQARIKQIKAQIEETSSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRAKAAIKDLKGANEDQTHGIQIALRAMEAP
                     LREIV"
     gene            complement(1470..1757)
                     /gene="groES"
                     /locus_tag="PK399_10055"
     CDS             complement(1470..1757)
                     /gene="groES"
                     /locus_tag="PK399_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPW13501.1"
                     /translation="MSNIKPLYDRVVIKRMEEEKLSAGGIVIPDSATEKPIKGEVIAV
                     GGGKVLDNGQLRTPQVKVGDKVLFGKYSGTEVKLDGTDLLVVKEDDIFAIL"
     gene            2010..4310
                     /locus_tag="PK399_10060"
     CDS             2010..4310
                     /locus_tag="PK399_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HPW13502.1"
                     /translation="MTALPNRLRRWLAALALLAALPAAAADIDLPPVDEVFVLSAQAT
                     ARDRIDVRWQIADGYYLYRHRTSVKADAAFANATLALPTGKKYRDEFFGDVETYRQQL
                     VGTLTGIPGAGATAAILTVKYQGCADAGVCYPPQTRTLKVILPGEAGDDAGGFGLGNR
                     KGGLFGVGGADAGATDALPLPVEQAFTSEVIAIDGNTLLLRLTPARGYYLYRDKLSLR
                     LDAGNGVSATLPPAAKLPKAQPYRDEHFGNVAVYFDQVEIPVPVARTVAAAARGTLVL
                     GLQGCQDGGICYPPMTRRLAVSLPAGTITPPAAATEPTITTAAADGAVDPGSASDAPI
                     ATATSADTAAPANDAGRTPPPPQQTRGLLASLVLALLGGLILNLMPCVLPVLSLKALS
                     LAQGGESQQRARRQALAYTAGVLASFAALGALALALRKAGLALGWGFQLQQPLVVALL
                     ALVVFAFGLSLSGLWYANVGIGQRGSALVQRDGVSGDFFTGVLAVVLATPCTAPFMGA
                     ALGYAFTGPTVGGMLVFLMLGLGLALPFLLIGFVPAFARLLPKPGAWMETLKQLLAFP
                     MYATAAWLLFVLASLRGADAVWHLGVAAILLAFAAWAWTRGRSGGSGHWQLVAALALL
                     GIAWPMVQLHALQKPQAATTQTHAGVPSTAFSEQALADLRAQGRVVFVNMTADWCVSC
                     KANEKVVFAREGFRTALDNANAVYMVGDYTDVDPAITAYLERHKAVGVPLYVVYPANG
                     GEGRILPVILTPGMAEDALAQAAAAR"
     gene            4337..4915
                     /locus_tag="PK399_10065"
     CDS             4337..4915
                     /locus_tag="PK399_10065"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPW13503.1"
                     /translation="MALLAAMAGAAASLYFEPTIARRLAGTEPGQRVLGAVLDAKAPA
                     PPAGVIVAKRGDIVPAFALATLEGTKTELPKAWAGRTTLVNFWASWCAPCLQEMPELE
                     AFSAQQGANGTQVVGIALDDPGPTQAMLERLGVTYPNLIDAPGPADAGVRLGNPAGVL
                     PYSVLVSAEGRVLKTKIGPFEHRQDIAAWARP"
     gene            5246..5686
                     /gene="aroQ"
                     /locus_tag="PK399_10070"
     CDS             5246..5686
                     /gene="aroQ"
                     /locus_tag="PK399_10070"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPW13504.1"
                     /translation="MAQLLVLHGPNLDLLGTREPEVYGRTTLADIDADLASRANAAGH
                     ALASLQSNAEHVLIERIHAARLDGTAFILFNPAAFTHTSVALRDALSAVAIPFIEIHL
                     SNPHTREPFRQHSYFSDKAVGVVCGFGADSYRYALDAALARLAA"
     gene            5704..6195
                     /gene="accB"
                     /locus_tag="PK399_10075"
     CDS             5704..6195
                     /gene="accB"
                     /locus_tag="PK399_10075"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HPW13505.1"
                     /translation="MDLRKIKKLIDLLEESNLAEIEIKEGEESVRLARVPKGGGYMQA
                     SAPAVQQVSAPAPAAPAPVMPMHSPSEAATGGSHKPHSDLPPGHVVRAPMVGTFYASP
                     SPDKPAFVSVGQAVKAGETLGIIEAMKMFNPIEADVSGTVLAIKCESGQPVEFDQPLF
                     VIG"
     gene            6289..>6382
                     /locus_tag="PK399_10080"
     CDS             6289..>6382
                     /locus_tag="PK399_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPW13506.1"
                     /translation="MLDKVVIANRGEIALRILRACHALGIRTVAV"
BASE COUNT          951 a         2240 c         2116 g         1075 t
ORIGIN      
        1 gacgatctcg cgcagcgggg cttccatcgc gcgcagggcg atctggatgc cgtgggtctg
       61 gtcttcattg gcgcccttca ggtccttgat cgcggccttg gcacggatca gggcgacacc
      121 accgcccggg acgatgcctt cctcgacggc cgcacgggtt gcgtgcaggg cgtcttcgac
      181 gcgcgccttc ttttccttca tctcgacttc ggtggccgcg ccgaccttga tcaccgccac
      241 gccgccggcc agcttggcca cgcgctcctg cagcttctcg cggtcgtagt cggagctggt
      301 ttcctcgatc tgcgccttga tctgcttgat ccgggcctgg atgccgtcgg catcgccggc
      361 gccgtcgatg atcgtggtgt tttccttggt gacctggatc ttcttggcgc ggccgaggtc
      421 cttgatcgtg gccttctcca gcgacaggcc cacttcctcg gaaatcacgg tgccgccggt
      481 cagggtggcc atgtcttcca gcatcgcctt gcgacgatcg ccgaagcccg gcgccttgac
      541 cgcgacgacc ttgacgatgc cgcggatggt gttgaccacc agggtcgcca gcgcctcgcc
      601 ttccacttcc tcggcgacga tcagcagcgg cttgccggcc ttggccacgc cttccagcac
      661 gggcagcagg tcgcgcacgt tggagatctt cttgtcgtgc agcaggatga acggatcgtc
      721 catctcggcc gacatcgact gctggttgtt gatgaagtac ggcgacaggt agccgcggtc
      781 gaactgcatg ccctcgacca cgtcgagttc gttgtccagg ccgctgcctt cctcgacggt
      841 gatcacgcct tccttgccga ccttgtccat cgcctgcgcg atcaggtcgc cgatgttggc
      901 gtcggagttg gcggagatcg cgccgacctg ggcgatttcc ttgctggtcg acgacggctt
      961 gctcagcgac ttcagttcgc cgacggcggc cttgaccgcc tggtcgatgc cgcgcttcag
     1021 gtccatcggg ttcatgccgg cggcgaccgc cttcatgccc tcgcggatga acgcctgcgc
     1081 cagcacggtc gcggtggtgg tgccatcacc ggcgttgtcg gaggtcttgg acgcgacttc
     1141 cttcaccatc tgcgcgccca tgttctcgaa ggcgtcggcc agctcgattt ccttggcgac
     1201 ggacacgccg tccttggtga tcgtcggcgc gccgaagctc ttctgcagca cgacgttgcg
     1261 gcccttcggg cccagggtgg ccttgacggc attggcgagc acgttgacgc cgcgcaccat
     1321 cttggcgcgt gcgtcttcgc cgaaacgaat gtcttttgca gccatgggga attacctcga
     1381 atgtgtgggg cgggcttcga cccgccgctc aggattgggg atgtttcgat ggcgggccgg
     1441 aacccgccag caacgaaaac gacgcgcgat cagaggatcg cgaagatgtc gtcttccttg
     1501 accaccagca ggtcggtgcc gtccagcttc acttccgtgc cgctgtactt gccgaacagg
     1561 accttgtcgc cgaccttcac ctgcggggtg cgcagctggc cgttgtccag caccttgcct
     1621 ccgccgacgg cgatgacttc gcccttgatc ggcttctcgg tggccgaatc cgggatcacg
     1681 atcccgccgg cggacaactt ctcttcttcc atgcgcttga tgaccacgcg gtcgtacagc
     1741 ggcttgatgt tggacatggc aacctcggta aatggttgat tgacagggga aaacccggcg
     1801 attttagcag tcgcccaagg cgagtgccaa accgcggacg agaaaaccgg gcgaacccgg
     1861 gatgccacgg agatggggca gcgcccaacc ctttcaaggg gggaggcgaa atccgcgtga
     1921 acccgcggac cgctgcgcgg tcgaagccgc accccggcgc agccagcggc cgcgggtgtc
     1981 ccgccgaccc cgcccggtaa actcatccga tgaccgcact tcccaaccgc ctgcgtcgct
     2041 ggctggccgc cctcgccctg ctggccgcct tgccggccgc cgccgcagac atcgacttgc
     2101 cgccggtcga cgaggtcttc gtcctgtcgg cgcaggccac cgcccgcgac cgcatcgacg
     2161 tgcgctggca gatcgccgac ggctactacc tgtaccggca ccgcacctcg gtcaaggcgg
     2221 atgccgcctt cgccaacgcg acgctggccc tgcccaccgg caagaagtac cgcgacgagt
     2281 tcttcggcga cgtcgaaacc taccgccaac aactggtcgg cacgctgacc ggtatcccgg
     2341 gcgccggcgc gaccgctgcc atcctgaccg tgaaatacca gggctgcgcg gatgccggcg
     2401 tctgctatcc gccgcagacc cgcacgctga aggtcatcct gccgggcgag gcgggggacg
     2461 atgcgggcgg tttcgggctc ggcaacagga agggcgggct gttcggcgtg ggcggtgccg
     2521 atgccggtgc caccgacgcc ttgccgctgc cggtcgaaca ggccttcacc agcgaagtca
     2581 tcgccatcga cggcaatacg ctgctcctgc ggctcacgcc ggcgcgcggc tattacctgt
     2641 atcgcgacaa gctctcgctg cgactcgatg ccggcaacgg tgtgtccgcc acgctgccgc
     2701 cagccgcgaa actgccgaag gcgcagccgt atcgcgacga acatttcggc aatgtcgcgg
     2761 tgtatttcga ccaggtcgag atcccggtgc cggtggcgcg gacggtggcg gctgccgcca
     2821 ggggcacgct ggtcctgggc ctgcagggct gccaggacgg cggcatctgc tatccaccga
     2881 tgacgcgccg gctggcggtg tcgctgcccg cggggacgat cacgccgccg gccgccgcga
     2941 ccgaaccgac catcaccacg gccgccgccg acggcgccgt cgacccgggc agcgcaagcg
     3001 acgcgcccat cgccacggcg acctcggccg acaccgccgc gcctgcaaac gacgcagggc
     3061 gcacgccacc gccgccgcaa cagacccgcg ggctgctggc atcgctggtt ctcgccctgc
     3121 tcggcggcct gatcctcaac ctgatgccct gcgtgttgcc ggtgctgtcg ctgaaggcgc
     3181 tctcgctggc gcaggggggt gaaagccagc agcgcgcgcg caggcaagcc ctggcctaca
     3241 ccgccggcgt gctggccagc ttcgccgcgc tgggcgcgct cgcgctggcg ttgcgcaagg
     3301 ccgggctggc gctgggctgg ggcttccagc tgcagcagcc gctggtggtc gcgctgctgg
     3361 cgctggtggt attcgccttc ggcctgagcc tgtccgggct gtggtacgcg aatgtcggca
     3421 tcggacagcg tggcagcgcg ctggtccagc gcgacggggt ctccggcgac ttcttcaccg
     3481 gcgtactggc ggtcgtgctg gccacgccgt gcacggcacc gttcatgggc gcggcgctgg
     3541 gctacgcgtt caccggaccc accgtcggcg ggatgctggt gttcctgatg ctgggcctgg
     3601 gcctggcgct gccgttcctg ctgatcggct tcgtccccgc cttcgcgcgt ttgctgccca
     3661 aaccgggggc gtggatggag acgctgaagc agctactcgc gttcccgatg tacgccaccg
     3721 ccgcgtggct gctgttcgtg ctggcgtcgc tgcgcggtgc cgacgccgtc tggcacttgg
     3781 gggttgccgc gatcctgttg gcattcgccg cctgggcctg gacccgtggc cgcagcggcg
     3841 gcagcggcca ttggcagctg gtcgcggcgc tcgccttgct cggcatcgcc tggcccatgg
     3901 tccagctcca tgccctgcag aaaccgcagg cggcgaccac gcaaacgcat gccggcgtgc
     3961 ccagcacggc gttctcggag caggccttgg ccgacctgcg ggcgcagggc cgcgtggtct
     4021 tcgtcaacat gaccgcggac tggtgcgtca gctgcaaggc aaacgagaag gtggtgttcg
     4081 cgcgtgaggg tttccgaacc gcactcgaca acgccaatgc cgtctacatg gtcggcgact
     4141 acaccgatgt cgatccggcg atcaccgcct atctggaacg ccacaaggct gtcggcgtgc
     4201 cgctctacgt ggtgtacccg gcgaacggtg gcgaaggccg gatcctgccg gtgatcctga
     4261 cccctggcat ggccgaagac gcattggccc aggccgcggc cgcccgctga tggcctccaa
     4321 caggacgatc gtggcggtgg ccttgctggc cgcgatggcc ggtgcggccg ccagcctgta
     4381 cttcgagccg accatcgccc ggcgcctggc cggcaccgag cccggccagc gcgtgctcgg
     4441 cgcggtcctc gatgcgaagg cgccagcgcc acctgcgggc gtgatcgtcg ccaaacgtgg
     4501 agacatcgtg ccggcattcg cgctcgcgac actcgaggga acgaaaaccg agctccccaa
     4561 ggcctgggcc ggcaggacca ccctggtcaa cttctgggcc agctggtgcg cgccctgcct
     4621 gcaggaaatg ccggagctgg aagcattctc cgcgcaacaa ggcgctaacg gcacgcaagt
     4681 cgtcggcatc gcgctggatg atccggggcc gacccaggcc atgctggagc gccttggcgt
     4741 cacctacccg aacctgatcg acgcccccgg ccccgccgac gcaggcgttc gccttggcaa
     4801 tccagcaggg gtgctgccct actcggtgct ggtttcggcg gagggccggg tgctcaagac
     4861 gaagatcggg ccttttgaac atcggcagga catcgcagcc tgggcacggc cctgacgggc
     4921 cggcgggcat gtgcaaagcg ggccatgggt gagcacgggc cgcttgcgcg gcactgccgc
     4981 gcgcccggac gaacgcccag cggcgcgccg ctgggccggg ggaggcgcgt agcgacgggc
     5041 ccgcggccgc gactcagtcc tggatgactg gacggagcgg ttggacatac ccgccggccc
     5101 gtcagggctg tgcgtgctga atcaggcatt tagagccttc gctcaaacaa gcgcttaaaa
     5161 ttctggaatc cgacgaaagt tcgccgaact ggacagcatc accggcctgc gcgacacttc
     5221 gcacccgttc cgacgtgctt tgtcgatggc gcaactgctg gtcctgcatg gccccaacct
     5281 cgacctgctc ggcacccgcg agccggaggt ctatgggcgc acgaccctgg ccgacatcga
     5341 tgccgacctg gcaagccgcg ccaacgccgc cggccacgca ctggcatcgc tgcagtcgaa
     5401 cgccgagcac gtgctgatcg aacgcatcca cgccgcccgc ctggacggca ccgccttcat
     5461 cctgttcaac ccggccgcct tcacccatac ctcggtcgcc ctgcgcgacg cgctgtccgc
     5521 ggtcgccatc cccttcatcg agatccacct gtcgaacccg catacccgcg aaccgttccg
     5581 ccagcacagc tatttcagcg acaaggcggt cggcgtggtc tgcgggttcg gtgccgattc
     5641 ctatcgctac gccctcgatg ccgcgcttgc gcggctcgcg gcctgaccca agaggaccgt
     5701 tccatggacc tgcgcaagat caagaagctg atcgacctgc tggaagaatc caacctggcc
     5761 gaaatcgaga tcaaggaggg cgaggaatcc gtgcgcctgg cgcgcgtccc caagggtggc
     5821 ggctacatgc aggcctccgc gccggcagtg cagcaggtgt cggcgcccgc acccgcggcg
     5881 ccagcaccgg tgatgccgat gcattcgccg tcggaggccg ccaccggtgg gtcgcacaag
     5941 ccgcacagcg acctcccgcc cggccacgtg gtgcgcgcgc cgatggtcgg caccttctac
     6001 gccagcccct cgccggacaa gccggccttc gtcagcgtgg gccaggcggt caaggccggc
     6061 gagaccctgg gcatcatcga ggcgatgaag atgttcaacc cgatcgaggc cgacgtctcc
     6121 ggcaccgtgc tggcgatcaa gtgcgaaagc gggcagccgg tcgagttcga ccagcccctc
     6181 ttcgtgattg ggtgagcagg gctttcgagc gccgacggcg cgagcctgcg aacgcccgcc
     6241 ggcctgcatg ccggcgggcc ggacgagcga acacattgag aaaccacgat gctcgataaa
     6301 gtagtcatcg ccaaccgcgg cgaaattgcg ctgcgcatcc tgcgcgcctg ccacgcgctg
     6361 ggcatccgca cggtcgccgt gc
//
LOCUS       DAOOCX010000123         6381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11384_length_6381_cov_7.611445, whole genome shotgun sequence.
ACCESSION   DAOOCX010000123 DAOOCX010000000
VERSION     DAOOCX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11384_length_6381_cov_7.611445"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PK399_10085"
     CDS             <1..447
                     /locus_tag="PK399_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HPW13507.1"
                     /translation="PSGHRNPDAGNRGPKKYGNKPRPQGGGFNPEAPRGPRPQGGFNA
                     DTRGPRREGGFNAGAGEGRPARAPRPYGHPGNAPSFPSDHANPGSFNPYADRPRGPRP
                     TGNKPGGARPNNAGPRGPRPGGNKPGGNRGGPRGGGGGGGSPYGNR"
     gene            473..880
                     /locus_tag="PK399_10090"
     CDS             473..880
                     /locus_tag="PK399_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13508.1"
                     /translation="MRSLAIVLLSLACLSPVQAQVAMPSGPTPLEYIDAKRLADADEK
                     SVTGAAHDAMLAAQRKLLDEGVVACSLGKPQEDFSGFTIVMALDASGHVQRTWREGGS
                     PLAICLQRYVRDKLVFVPPRAPFHTSLAISFTR"
     gene            1030..1953
                     /locus_tag="PK399_10095"
     CDS             1030..1953
                     /locus_tag="PK399_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPW13509.1"
                     /translation="MQATETGIPPIREGSSTMGTKKILNVTSAPGRHWVGNGFPVHGM
                     FGYSGPGVAERSPFLMLDYAAPFEFQPTRERRGVGQHPHRGFETVTIVYEGEVEHRDS
                     TGNGGIIGKGDVQWMTAAGGILHEEFHSPGYAERGGPFEMVQLWVNLPRKDKMAAAGY
                     QGITDAQIPAVALPADAGTLRVIAGDYDGHRGPARTFSPINVWDVRLAAGKTIALPQP
                     EGWTTLVVVLAGTVELNGETVLRPAQMATLSTAGTGVTIETSGDAKVLVLAGEPIDEP
                     VVGYGPFVMNSEQEIRQAIADFNSGKFGNMQ"
     gene            2037..2465
                     /locus_tag="PK399_10100"
     CDS             2037..2465
                     /locus_tag="PK399_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPW13510.1"
                     /translation="MAVSTVRASIGATPYTVELSDNHGHRWLGDEPVDLGGANAGPAP
                     EQQLLASLGTCTAVTLMMYARRKQWLLTQVHVDLAFNPQGKPPSGSDIHRSIQLIGQL
                     DDEQRARLMQIADACPIHKVLTGEIRIATISAHDDTGASA"
     gene            2462..3022
                     /locus_tag="PK399_10105"
     CDS             2462..3022
                     /locus_tag="PK399_10105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HPW13511.1"
                     /translation="MTRLLGISGSLRAASFNTALLRAAKEVAGSGIDLDIATLHGIPL
                     YDGDAEARDGEPAAVTALKQRILASDGVLLVTPEYNNGIPGVFKNGIDWLSRGTPDIP
                     AVFGDRPFALIGASPGGFGTILSQAHWLPVLKTLGVRLWAGQKLLVSRAGSVFDADGA
                     LVDDKVRTQLAGFVHGFAAFAGHARD"
     gene            3233..5134
                     /locus_tag="PK399_10110"
     CDS             3233..5134
                     /locus_tag="PK399_10110"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW13512.1"
                     /translation="MRADQTRTAAQLEAQAARLRASTADPRFRAWATLAEAEFANDQE
                     HADEAMAGLDQAIAQADALKLPDLRFEALIRLSTVLVNRGRSKDTEAVLAKMQAMVEA
                     GGNTRWRAQWLHERGVLERKLGRFEQARALFVQARALFHGIGDPVMEAREMNSIGNLD
                     GRTGRFSDAVAMHSDALTMARKAGDKAETARSLRMLGVLYLNLDDEELASRYLLEALD
                     YVEERNRREAIALHGELIGTFTRLEKLTEAEYHGQQAVRLAELSGSHPNRVNAFTRMA
                     ELRLLQGRIDEAEQWVARANESRDHVAVRDRSLIGISRMRVLAARDRTAAALKEADAV
                     LADVRDLGDRILERSVLDLMSELQLRAGDAASAFATRKAHQKLDKELAIDVAARRIAL
                     LESSLERERAKTERELLQRDNDIQALRLNRQRLIAIALLVGLATVLVVSALLYARYRS
                     TLRHRNELRASRDALERVANTDALTGLANRHAAARALGERLGRIPEPLTVMLLDLDEF
                     KRINDSHGHQAGDAVLQEAAKRMRAALPEDALLGRWGGEEFIAILDGVPGREPASVAE
                     AMRAALAAAPVLFEGTAIPVTVSIGVATAFAADGIDTLLAEADAALYRAKGAGRNRVV
                     YGRAPVSLA"
     gene            complement(5138..5944)
                     /locus_tag="PK399_10115"
     CDS             complement(5138..5944)
                     /locus_tag="PK399_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPW13513.1"
                     /translation="MNNLRVSIVQGETRWHDPAGNRDYYGELIAPLHGITDLVVLPET
                     FTSGFSNDAIGNAETMDGPTVDWIREQALKLDAAVTGSVQLRTEAGVFNRMLFATPDG
                     GLTHYDKRHLFSFAKEHERYAAGRERVVVAWKGWNILLQVCYDLRFPVFARNRFNVDR
                     ADAPDYDLALYVANWPSARAYPWKTLLRARAIENLCYVVGVNRVGTDGNGLHYSGDSA
                     VIDFLGHAVSEATDEEVVTTTTLQAAELAAHRQRFPAMFDGDAFRLLDEG"
     gene            complement(5941..>6381)
                     /locus_tag="PK399_10120"
     CDS             complement(5941..>6381)
                     /locus_tag="PK399_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007964632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW13514.1"
                     /translation="GWKIGYCIAPPALSAEFRKVHQYNVFCTFAPAQHAFAEMLVAEP
                     EHYEGLGAFYQAKRDRFREQLLATRLRPLAVPGGYFQLVDYSAVSDLDDAAFCRWLTI
                     EKGVAAIPLSPFYETPPAGQRLARLCFAKNDATMDAAIERLQAL"
BASE COUNT         1059 a         2243 c         2172 g          907 t
ORIGIN      
        1 cccagcgggc atcgcaaccc ggacgccggc aaccgcggtc cgaagaagta cggcaacaag
       61 ccgcgtccgc agggcggcgg cttcaatccg gaggcgccgc gtggcccgcg tccgcagggc
      121 ggcttcaatg ccgacactcg cggaccgcgt cgcgaaggcg gtttcaatgc gggtgccggc
      181 gaagggcgtc cggcgcgcgc gccgcgtccg tacggtcatc ccggcaatgc gcctagcttc
      241 ccgtccgacc acgccaaccc gggcagcttc aatccgtatg ccgatcgccc gcgcggccca
      301 cgccccaccg gcaacaagcc gggcggcgcg cgcccgaaca acgccggccc gcgcggcccg
      361 cgtccgggcg gcaacaagcc cggcggcaac cgcggcggcc cgcgcggtgg cggtggcggt
      421 ggcggcagcc cgtacggcaa tcgctgatcg atcccgacaa ggaagcctgc tgatgcgcag
      481 cctcgccatc gtcctgctgt cgctcgcctg cctgtcgccc gtacaggcgc aggtcgcgat
      541 gccgtccggg cccacgccgc tggagtacat cgacgccaag cgcctggccg atgccgatga
      601 aaaaagcgtc accggcgctg cgcatgacgc gatgctggcc gcgcaacgca agctgctgga
      661 cgaaggcgtg gtcgcatgct cgcttggcaa gccgcaggag gatttcagcg gcttcaccat
      721 cgtcatggca ctggatgcca gcgggcacgt gcagcgcacc tggcgcgaag gcggttcgcc
      781 cctggcgatc tgcctgcagc gctacgtccg cgacaagctg gtcttcgtgc cgccgcgcgc
      841 gcccttccac acctcgctgg cgatcagctt caccaggtga ccggccgtca tcgatgccac
      901 gaggggcgcc cattgggcgc ccttttccat ttgcgcggtc atcccaggca acgagccgtg
      961 attgattgca aatatggaac aaatcggtat cgattgaccg actagatcca tccatgcaca
     1021 caatccaaga tgcaggccac ggaaaccggc atcccgccga tccgcgaagg gagcagcacc
     1081 atgggcacca agaagatcct caacgtcacc agcgcccccg gccggcattg ggtcggcaac
     1141 ggcttcccgg tgcatggcat gttcggctac agcggtccgg gcgtggccga gcgcagcccg
     1201 ttcctgatgc tggactacgc cgcccccttc gagttccagc ccacccgcga acgacgcggc
     1261 gtcggccagc acccgcaccg tgggttcgag accgtcacca tcgtctacga gggcgaggtc
     1321 gagcaccgtg attccaccgg caacggcggg atcatcggca agggcgacgt gcagtggatg
     1381 accgccgcgg gcgggatcct ccacgaggag ttccactcgc ccggttatgc cgagcgcggg
     1441 gggccgttcg agatggtgca gctgtgggtc aacctgccga ggaaggacaa gatggccgcg
     1501 gccggctacc agggcatcac cgatgcacag attcccgcgg tcgccctgcc cgccgatgcg
     1561 ggcaccctgc gggtgatcgc cggcgactac gacggccatc gcggcccggc ccgcaccttc
     1621 agcccgatca acgtctggga tgtgcggctg gcggccggca agaccatcgc gttgccgcag
     1681 ccggaaggct ggaccacgct ggtcgtggtg ctggcgggca cggtggaact caacggtgaa
     1741 accgtgttgc ggcccgcgca gatggcgacc ctgtccacgg ccggcaccgg cgtgaccatc
     1801 gaaaccagcg gcgatgccaa ggtgctggtg ctggccggcg agccgatcga cgagccggtg
     1861 gttggctacg gcccgttcgt gatgaacagc gagcaggaaa tccgccaggc gatcgccgac
     1921 ttcaacagcg ggaagttcgg caacatgcaa tgagcggcat gcctccgtgg tcccggtaca
     1981 cgccgggatc acggccacgc gcttcaatgc gcaacgaatg accgggagtt acacgcatgg
     2041 ctgtttccac cgtccgcgcc agcatcggcg ccacgcccta caccgtcgaa ctcagcgaca
     2101 accacggcca ccgctggctg ggcgacgaac ccgtggacct gggcggcgcg aatgcgggcc
     2161 cggccccgga gcagcagttg ctggcctcgc tgggtacctg caccgcggtg accctgatga
     2221 tgtacgcccg gcgcaagcag tggctgctca cccaggtgca cgtggacctg gccttcaatc
     2281 cgcaaggcaa gccgccctcc ggcagcgaca tccaccgcag catccagttg atcgggcaac
     2341 tcgacgacga gcaacgcgcc cggttgatgc agatcgccga tgcctgcccg atccacaagg
     2401 tgctgaccgg cgagatccgc atcgccacca tttccgccca tgacgacacc ggagcgtccg
     2461 catgacccgc ctcctcggca tttccggcag cctgcgcgcc gcctcgttca acaccgccct
     2521 gctgcgtgcg gcgaaagagg ttgccggcag cggcatcgac ctggacatcg ccaccctgca
     2581 cggcatcccg ctctacgacg gcgacgccga agcgcgcgac ggcgagcccg ccgcggtcac
     2641 cgcgctcaag cagcgcatcc tcgccagcga tggcgtgctg ctggtcacgc cggaatacaa
     2701 caacggcatc cccggcgtgt tcaagaacgg catcgactgg ctgtcgcgcg gcaccccgga
     2761 catccccgcg gtgttcggcg accgtccgtt cgcgctgatc ggggcctcgc cgggcgggtt
     2821 cggcacgatc ctgtcgcagg cccactggtt gccggtgctg aagacgctcg gcgtgcgcct
     2881 gtgggccggg cagaagctgc tggtctcgcg cgccggcagc gtgttcgatg ccgacggcgc
     2941 gctggtcgac gacaaggtgc gcacgcaact ggccggcttc gtgcacgggt tcgcggcgtt
     3001 cgcgggtcac gcgcgggact gacgcgcacc ggcccactac gccgcccgcc ccgactctgg
     3061 catgctgcca cgcaggatcg gggggaaccc ggacatgggc aggggtggca tcggtcggaa
     3121 aggcgggtcc tggtggcgct gcggcgtcct ggcgaccgcg ttctggctat gcctggccgt
     3181 tgcatccggc gcaggtgccg ccaaccccag tgaacctgcc gcgatcgcgg cgttgcgtgc
     3241 cgaccagacc cgaacggcgg cgcaactcga agcacaggcc gcgcgcctgc gcgccagcac
     3301 cgccgacccc aggttccgcg cctgggccac gctggccgaa gccgaattcg ccaacgacca
     3361 ggaacacgcc gatgaggcga tggcgggcct cgaccaggcc atcgcgcagg cggatgcgct
     3421 gaagctgccg gacctgcgtt tcgaggccct gatccggctc tcgaccgtgc tggtcaaccg
     3481 cggacgctcg aaggacaccg aagccgtgct ggcgaagatg caggccatgg tcgaggccgg
     3541 cggcaatacc cgctggcgcg cgcagtggct gcacgagcgc ggcgtactgg agcgcaagct
     3601 gggccgcttc gagcaggccc gcgccctgtt cgtgcaggcg cgggcgctgt tccatggcat
     3661 cggcgatccg gtgatggaag cccgcgagat gaattccatc ggcaacctgg acggccgcac
     3721 cgggcgcttc tccgatgccg tggcgatgca cagcgacgcc ctgaccatgg cccgcaaggc
     3781 cggcgacaag gccgagaccg cgcgcagcct gcgcatgctc ggcgtgctct acctcaacct
     3841 ggacgacgag gaactggcca gccgctacct gctggaggcg ctggactacg tggaggaacg
     3901 caaccgccgc gaggcgatcg ccctgcacgg cgaactgatc ggcaccttca cccggctgga
     3961 aaaactgacg gaagcggagt accacggcca gcaggcggtg cgcctggccg aactgtccgg
     4021 cagccatccc aaccgggtca atgcgttcac ccgcatggcg gaactgcgcc tgctgcaggg
     4081 gcgcatcgac gaggcggagc aatgggtcgc gcgggcgaac gagtcccgcg accacgtcgc
     4141 ggtccgcgac cgcagcctga tcggcatcag ccgcatgcgc gtgctcgccg cgcgcgaccg
     4201 caccgcagct gccctgaagg aagcggatgc ggtgctggcc gacgtgcgcg acctgggcga
     4261 ccggatcctg gaacgctcgg tgctggacct gatgtcggaa ctgcagttgc gcgccggtga
     4321 cgccgccagc gcgttcgcca cccgcaaggc gcaccagaag ctggacaagg agctggcgat
     4381 cgacgtcgcc gcgcgccgga tcgcgctgct ggaatccagc ctggaacgcg agcgcgcgaa
     4441 gaccgaacgc gaactcctgc agcgcgacaa cgacatccag gcgctgcggc tgaaccgcca
     4501 gcggctgatc gcgattgccc tgctcgtcgg gctggccacg gtgctggtgg tgtccgccct
     4561 gctctacgcg cgctaccgca gcaccctgcg ccatcgcaac gaattgcgcg ccagccgcga
     4621 cgcgctggag cgcgtggcca ataccgacgc actgaccggg ctcgccaacc gccacgctgc
     4681 cgcgcgtgcg ctgggcgaac gcctgggtcg catcccggaa ccgctgacgg tgatgctgct
     4741 ggatctcgac gagttcaagc gcatcaacga cagccacggc caccaggccg gcgatgcggt
     4801 gctgcaggaa gcggcgaagc gcatgcgcgc cgcattgccg gaggacgcgc tgctggggcg
     4861 ctggggcggc gaggaattca tcgcgatcct ggacggcgtg ccgggccgcg aaccggcctc
     4921 ggtcgccgag gcgatgcgcg ccgcactcgc ggccgcgccc gtgctgttcg agggcacggc
     4981 gatcccggtg accgtgagca tcggcgtggc caccgccttc gccgccgacg gcatcgacac
     5041 cctgctggcc gaggccgatg ccgcgctcta tcgcgccaag ggcgccggcc gcaaccgcgt
     5101 ggtctacgga cgcgcgccgg tctcgctggc ctgagcctca gccctcgtcg agcaggcgga
     5161 acgcatcgcc atcgaacatc gccgggaaac gctggcgatg ggcggccagc tcggcggcct
     5221 gcagcgtggt ggtggtcacc acttcctcgt ccgttgcctc gctgacggca tggccgagga
     5281 aatcgatgac cgcactgtcg ccgctgtagt gcaggccatt gccgtcggtg ccgacgcggt
     5341 tcacgccgac cacgtagcag aggttctcga tggcgcgcgc gcgcagcagg gtcttccacg
     5401 gatacgcacg cgccgacggc cagttcgcca cgtacaacgc caggtcgtag tccggcgcat
     5461 ccgcgcgatc cacgttgaag cgattgcgcg cgaacaccgg gaaacgcagg tcgtagcaga
     5521 cctgcagcag gatgttccag cctttccagg ccacgaccac gcgctccctg ccggcggcgt
     5581 agcgctcgtg ttccttggcg aaggaaaaca ggtggcgctt gtcgtaatgg gtgagcccgc
     5641 cgtcgggcgt ggcgaacagc atgcggttga atacacccgc ttcagtccgc aattgcacgc
     5701 tgccggtgac ggcggcatcg agcttcaacg cctgttcgcg gatccagtcc acggtcggcc
     5761 cgtccatggt ttcggcattg ccgatcgcat cgttggagaa gccgctggtg aaggtctccg
     5821 gcagcaccac caggtcggtg atcccatgca gcggcgcgat cagttcgccg tagtagtcgc
     5881 gattgccggc ggggtcgtgc cagcgggttt cgccctggac gatggagacg cggaggttgt
     5941 tcatagcgcc tgcaaccgct cgatcgccgc atccatcgtc gcatcgttct tggcgaagca
     6001 cagacgcgca aggcgctgcc cggccggcgg ggtttcgtag aacggcgaca gcgggatcgc
     6061 ggccacgccc ttctcgatgg tcagccagcg gcagaacgcg gcatcgtcga ggtcgctgac
     6121 cgccgagtaa tcgaccaact ggaaataccc acccggcacc gccagcggcc tgagcctggt
     6181 cgccagcagt tgctcgcgga agcggtcgcg cttggcctgg tagaacgcgc cgagtccttc
     6241 gtagtgttcc ggctcggcca ccagcatttc ggcgaaggcg tgctgcgccg gggcgaaggt
     6301 acagaacacg ttgtactggt ggaccttgcg gaactccgcg ctcaaggccg gcggcgcgat
     6361 gcagtagccg atcttccagc c
//
LOCUS       DAOOCX010000124         6354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11479_length_6354_cov_8.432132, whole genome shotgun sequence.
ACCESSION   DAOOCX010000124 DAOOCX010000000
VERSION     DAOOCX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11479_length_6354_cov_8.432132"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PK399_10125"
     CDS             complement(<1..272)
                     /locus_tag="PK399_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HPW13515.1"
                     /translation="MSSYLFTSESVSEGHPDKIADQISDAVLDAILAQDKRARVACET
                     MVKTGAAIVAGEVTTSAWVDIEALARKVINDIGYDNSDVGFDGHTCA"
     gene            488..2224
                     /locus_tag="PK399_10130"
     CDS             488..2224
                     /locus_tag="PK399_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014645963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW13516.1"
                     /translation="MLQLEQRLRQRFPHWFRGRRAAIARPLVRGIARWSRLDAIETFL
                     TANRHVRGFDFVSAALQFLGADYTVDAAALARIPAQGRLLVVANHPSGALDALALLDA
                     IGKVRRDVRIVANDMLSALDNLDGLLLPVRILGGRPTPDSVRAVDTALARGECVIVFP
                     AGEVSRLGPRGIRDSRWRRGFLRFARACDAPVLPVRIHARNSALFYGASALFKPAGTA
                     LLAREMFARRGRRIRLLVGESIRLDAGADDAGELQRVRRMLYALRVHPHPNPLPPAGE
                     GEEHPEPLVDAVDPAQVRMGVAGLRLLGETGDGKQIRVGTLAVDAPLLGEIGRLRELT
                     FREVGEGTGRSVDLDAWDSWYEHIVLWDDAASKVVGAYRIARGAPVLAAHGIRGLYTA
                     SLFDYADDAIARIAQGMELGRSFVVPEYWGSRSIEYLWQGIGAYLRTHPKVRYLFGAV
                     SISAALPMAAREQIAAHYAHYFGGGTGEVLSKRPFAYQAAMPQDAAMDADTAFRVLKA
                     NLDALGAQVPMLYKQYTELCEPGGARFLAFGVDPGFSDAVDGLIEVDIQRMRPKKRQR
                     YLGEVAEAGAMA"
     gene            2221..3048
                     /locus_tag="PK399_10135"
     CDS             2221..3048
                     /locus_tag="PK399_10135"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPW13517.1"
                     /translation="MSLQAAPPMRRAVFLSDVHLGSRHCHAAELAAFLSTLRCKRLYL
                     VGDIVDLWWMAQRRAQWGSAQNRVVEALHALRRAGTEIVYIPGNHDRPIRRFCGLALP
                     AMQVRRRAIHVTADGRRLLVVHGDEFDGVTHFGNLQEKFGDWLYYRILSGNRLLNIVR
                     GGLGLRYWSLSEFLKSKSGAAERFIERFMQAGLDDARRRGLDGIVCGHIHRAALLQRG
                     GLVYANDGDWVESLSALVEDADGTLRLLAHTGEVLAELLPRSLSQGAAVPVALPRAA"
     gene            3081..4136
                     /locus_tag="PK399_10140"
     CDS             3081..4136
                     /locus_tag="PK399_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPW13518.1"
                     /translation="MDSITHLFYGGVIAAAIAPRQHRRAALLAGMVLNTLPDLDVLPL
                     LLSDDPIVRMTWHRAATHSWFVLPLVAWGIWAFFRRRGGRVAQAPTRWWWAIFACLMA
                     HPLLDSFTVYGTQLLWPLPVPPLMWSSLFIIDPLFTAAWLAAFVAAMVAGSRQWGGKA
                     LAAGIVLGVGYVGWSLLAKSLVQREADRALAAMGLADAPRFSVPMPFNTLLWRVVAMT
                     PSGFVEGERSLVADHGPMVFRGYPSHVQALRQAAAIPAVQELGWFNHGFQKAQVRDGQ
                     LVLSDLRMGAEPDYSFNFAVAEPDGDGWRAIPPQQLRWPWEASRRLGDMWQRIWQQPA
                     AQAAASSGETPAASASK"
     gene            complement(4085..5080)
                     /gene="dusB"
                     /locus_tag="PK399_10145"
     CDS             complement(4085..5080)
                     /gene="dusB"
                     /locus_tag="PK399_10145"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPW13519.1"
                     /translation="MLIGPHRIEPKVILAPMAGVTDKPFRLLCKRLGAGLAVSEMTTS
                     DPRFWTTAKSLHRMDHVGEPDPVSVQIAGTVPAIMADAARFNVDHGAQLIDINMGCPA
                     KKVCNAWAGSALMREPDLVARIVAAVVNAVDVPVTLKIRTGWDAGHRNAPEIARIAED
                     AGIQALAVHGRTRDQQYAGTAEYDTIAAIKAMLRIPVIANGDIDSPEKARQVLEATGC
                     DAVMIGRAAQGRPWIFGQVAHYLATGERLPDPSLHEVRDILLAHLGQLHAFYGEVSGV
                     RIARKHLGWYAKDRPENAAFRAVVNQAQTADEQLRLTRDYFDALAAGVSPELAAA"
     gene            complement(5233..6177)
                     /locus_tag="PK399_10150"
     CDS             complement(5233..6177)
                     /locus_tag="PK399_10150"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011409356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPW13520.1"
                     /translation="MNRVVVAGSFNVDHVWRVPALPQAGATLSGDYTTGPGGKGFNQA
                     VAARRAGAGTCFVCALGDDLGAQLARVLCTADGIDLRDVVVQAPTGTAGIYVDAQGRN
                     SIVIGAGANAALQPAFVAEALAGLQPGDVLVAQLESPLDAVAKALDAGRAAGATTLLN
                     PAPANVAIDHAMLERADILTPNETEFAAMLGRHVGARVEADAVSGIDQQRLHALCRDL
                     THGTVVVTLGASGCFVSHADANLRGDATACYRVPAEAARVVDTTGAGDAFNGALAASM
                     ALKPERAFAEHVRFAGRYASRSTELYGAALAMPRMPVE"
     gene            complement(6182..>6354)
                     /locus_tag="PK399_10155"
     CDS             complement(6182..>6354)
                     /locus_tag="PK399_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HPW13521.1"
                     /translation="CGFANFSSIAIQIGGIGGLAPERRSDLARFGLRAVLGGSIATFM
                     TATIAGVLSYFG"
BASE COUNT          875 a         2138 c         2276 g         1065 t
ORIGIN      
        1 gcgcaggtgt ggccgtcgaa gccgacgtcg gaattgtcgt agccgatgtc gttgatgacc
       61 ttgcgcgcca gcgcctcgat gtcgacccag gcgctggtgg tgacttcgcc ggcgacgatg
      121 gcggcgccgg tcttcaccat ggtctcgcag gccacgcggg cgcgcttgtc ctgcgccagg
      181 atcgcgtcga ggacggcgtc ggaaatctgg tcggcgatct tgtccgggtg gccttcgctg
      241 accgactcgg aggtgaagag gtagctggac atcgcggctc ctgtgagtga ttcatccttg
      301 tatgcggatg aaaggatggc cgcgcatgat acagctgtgc gggcccgcgc gcatcttgcg
      361 ccatcctgcg ggggatgggt attgaagttg cgttaagcgg gatcgtcatc tccccgcaac
      421 ctgacggcaa cggcggcgtc gcatcggcgg cgcaggctgc cggccaacac ggtcgcggcc
      481 cgtcgccatg ctccagctcg aacagcgcct ccgccagcgt ttcccgcact ggttccgtgg
      541 acgccgcgcg gccatcgccc ggccgttggt gcgcgggatc gcgcgctggt cgcggctgga
      601 tgccatcgag accttcctca ccgccaaccg gcacgtgcgc ggcttcgatt tcgtgtccgc
      661 ggccctgcag ttcctcggcg cggactacac ggtggacgcc gccgcgctcg cgcgcattcc
      721 cgcgcagggt cgcttgctgg tcgtcgccaa ccatcccagc ggcgcgctgg atgcgctcgc
      781 gttgctcgat gccatcggca aggtgcgccg cgatgtgcgc atcgtcgcca acgacatgct
      841 ctccgcgctc gacaacctcg acggtttgtt gctgccggtc cgcatcctcg gcggtcgacc
      901 cacgccggac agcgtgcgtg cggtcgatac ggcgctggca cgcggcgaat gcgtgatcgt
      961 gttcccggcg ggcgaggtct cgcggcttgg cccgcgcggc atacgcgact cgcgttggcg
     1021 gcgtggcttc ctgcgcttcg cccgcgcctg cgacgcgccc gtgttgccgg tgcgcatcca
     1081 cgcgcgcaat tcggcgctgt tctacggggc atcggcattg ttcaagcccg ccgggaccgc
     1141 cttgctggcg cgagagatgt tcgcgcggcg cggccggcgg atccgcttgc tggtgggcga
     1201 gtcgatcagg ctcgatgccg gcgcggatga cgccggcgaa ctgcagcgcg tgcgacgcat
     1261 gttgtatgcg cttcgcgtcc accctcaccc caaccctctc ccgccagcgg gagaggggga
     1321 agagcatccc gagcctctcg ttgatgcggt ggatccggca caggtgcgca tgggggtggc
     1381 cggcctgcgt ctgctcggcg agaccggcga cggcaagcag atccgcgtcg gcacgctggc
     1441 ggtggacgcg ccgctgttgg gcgagatcgg ccgcctgcgc gagctgacct ttcgcgaagt
     1501 gggggagggc accggtcgca gcgtggacct ggatgcctgg gacagctggt acgagcacat
     1561 cgtgctgtgg gatgacgcgg cgtcgaaggt agtcggggcc taccgcatcg cccgcggcgc
     1621 accggtactg gccgcgcacg gcatccgcgg cctgtacacc gcatcgttgt tcgattacgc
     1681 cgacgatgcc atcgcccgga tcgcacaggg gatggaactc ggtcgtagct tcgtggtgcc
     1741 cgagtactgg ggaagtcgca gcatcgaata cctgtggcag ggcatcggtg cctacctgcg
     1801 cacgcatccg aaggtgcgct acctgttcgg cgcggtctcg atcagcgccg cgctgccgat
     1861 ggcggcacgc gagcagatcg ccgcgcatta cgcgcactac ttcgggggcg gcaccggcga
     1921 ggtcctgtcg aagcgtccgt tcgcctacca ggccgccatg ccgcaggacg cggcgatgga
     1981 tgcggatacc gcgttccgcg tgctgaaggc gaacctggat gcgctcggcg cgcaggtgcc
     2041 gatgctctac aagcagtaca ccgagctgtg cgagccgggt ggtgcgcgct tcctcgcgtt
     2101 cggggtggac ccgggcttca gcgatgcggt ggacgggttg atcgaagtcg acatccagcg
     2161 catgcggccg aagaagcgcc agcgctacct gggcgaagtg gctgaggcgg gggcaatggc
     2221 atgagcctgc aggccgcgcc gccgatgcgt cgcgcggtct tcctctccga cgtccacctg
     2281 ggctcgcgcc attgccacgc ggcggaactg gccgcgttcc tgtccacctt gcgctgcaag
     2341 cgcctgtacc tggtcggcga catcgtcgac ctgtggtgga tggcgcagcg tcgcgcgcaa
     2401 tggggcagcg cgcagaaccg ggtggtggag gcgctgcacg cgctgcgtcg cgccggcacc
     2461 gagatcgtct acatccccgg caaccacgac cgcccgatcc gccgcttctg cgggctggca
     2521 ttgccggcga tgcaggtgcg ccgtcgcgcg atccacgtca ctgcggatgg ccgccgcctc
     2581 ctggtcgtgc atggcgacga gttcgatggc gtcacccact tcggcaacct gcaggagaag
     2641 ttcggcgact ggctgtacta ccggatcctg agcggcaacc gcttgttgaa tatcgtgcgt
     2701 ggtggcttgg gcctgcgcta ctggtcgcta tcggagttcc tgaagtcgaa gagcggtgcg
     2761 gcggagcgtt tcatcgaacg cttcatgcag gccgggctgg acgacgcgcg ccgtcgcggg
     2821 ctggacggca tcgtctgcgg ccacatccat cgcgccgcgc tgctgcagcg tggtggcctg
     2881 gtgtacgcca atgacggcga ctgggtggag agcctcagcg ccttggtcga ggacgcggat
     2941 ggcaccttgc gcctgctggc gcataccggc gaggtgctgg cggaactact gccgcgatcg
     3001 ctgtcgcagg gagctgcagt gcccgtggca ttgccgcgcg cggcgtgatc gcgatggagg
     3061 atgccggtta gcatccgcgg atggattcca tcacccatct tttctatggc ggcgtgatcg
     3121 ccgccgcgat cgcaccgagg cagcatcgtc gcgccgcgct gctggccggc atggtgctca
     3181 acacgctgcc cgacctcgac gtgctgccgc tgctgctcag cgacgacccc atcgtgcgca
     3241 tgacctggca ccgcgcggcg acccattcct ggttcgttct gccgctggtg gcatggggga
     3301 tctgggcgtt cttccggcgt cgcggcggtc gcgtggcgca agcgccgaca cgctggtggt
     3361 gggcgatctt cgcctgcctg atggcgcatc cgttgctgga cagcttcacc gtctacggca
     3421 cccagctgtt gtggccgctg ccggtcccgc cgctgatgtg gagcagcctg ttcatcatcg
     3481 atccgctgtt cacggcggca tggcttgcgg cgttcgtggc ggcgatggtc gccggcagcc
     3541 ggcagtgggg cggcaaggcg ctcgccgccg gcatcgtgct tggcgtcggc tacgtgggct
     3601 ggtcgctgct ggcgaagtcg ctggtgcagc gcgaggcgga tcgcgcgctc gccgcgatgg
     3661 gcctggccga tgcgccgcgc ttctcggtgc cgatgccgtt caacacgctg ctgtggcgag
     3721 tggtggcgat gacgccatcg gggttcgtcg aaggcgagcg ttcactggtc gccgaccacg
     3781 ggccgatggt gttccgtggc tatccctccc atgtgcaggc gctgcgccag gcggccgcga
     3841 tcccggccgt gcaggaactc ggctggttca accacggctt ccagaaggcg caggtccgcg
     3901 acggccaact ggtactcagc gacctgcgga tgggcgcgga gcccgactac agcttcaatt
     3961 tcgcggtggc cgaacccgat ggcgacggct ggcgcgcgat cccgccgcaa caactgcgct
     4021 ggccgtggga ggcatcgcgc aggctgggcg acatgtggca gcgcatctgg cagcagcccg
     4081 cggctcaggc cgcggcgagt tccggcgaaa cgcccgcggc cagcgcgtcg aaataatcgc
     4141 gggtcagccg cagttgttcg tccgcggtct gcgcctggtt gaccaccgcg cggaacgccg
     4201 cgttctcggg ccggtccttc gcataccagc ccaggtgctt gcgggcgatg cgcacgccgg
     4261 atacctcgcc gtagaacgca tgcagctgtc cgagatgcgc gagcaggatg tcgcggacct
     4321 catgcagcga gggatctggc agccgttcgc cggtggccag gtagtgcgcg acctggccga
     4381 agatccacgg ccggccctgt gcggcgcgtc cgatcatcac cgcatcgcag ccggtcgcct
     4441 ccagcacttg gcgcgccttc tccggcgaat cgatgtcgcc gttggcgatc accgggatcc
     4501 gcagcatcgc cttgatcgcg gcgatggtgt cgtactcggc cgtgccggcg tactgctggt
     4561 cgcgggtgcg gccgtgcacg gccagcgcct ggatgccggc gtcctcggcg atgcgggcga
     4621 tctcgggtgc attgcgatgc ccggcatccc agccggtgcg gatcttcagg gtcaccggta
     4681 cgtcgacggc attcaccacc gcagcgacga tgcgcgcgac caggtcgggt tcgcgcatca
     4741 gggcgctgcc ggcccaggcg ttgcagacct tcttggccgg gcagcccatg ttgatgtcga
     4801 tgagttgcgc gccgtggtcc acgttgaagc gggcggcatc ggccatgatc gcgggcacgg
     4861 tgccggcgat ctgcacgctg accggatccg gttcaccgac atggtccatc cggtgcaggg
     4921 atttcgcggt ggtccagaag cgcggatcgg aggtggtcat ttccgacacc gccaagcccg
     4981 cgcccaatcg cttgcacagc agtcggaacg gcttgtcggt gacgcccgcc atcggggcga
     5041 ggatcacctt cggttcgatg cggtgcgggc cgatcagcat ccgtgcattg taggacgcgc
     5101 cccctttccc gatgcgctgc gcgcatcgag ccggcccggc cagccggcct gcatgtcggc
     5161 tggcgttcgc aggctcgcgg cgtcgccgct cgaattccct ggtcaccccg caaggggaga
     5221 ggtggaacag gtttattcga ccggcatccg tggcatcgcc agcgcggcgc catacaactc
     5281 ggtggagcgc gaggcgtagc gtcccgcgaa gcgcacgtgc tcggcaaagg cgcgctccgg
     5341 cttcagcgcc atcgacgcgg ccagcgcacc gttgaaggca tcgcccgcgc cggtggtgtc
     5401 caccacgcgc gccgcttccg ctggcacgcg gtagcaggca gtcgcatcgc cgcgcaggtt
     5461 cgcgtccgca tgcgacacga agcagccgct ggcgcccagg gtgaccacca cggtgccgtg
     5521 ggtcaggtcg cggcacagcg catgcagccg ctgctggtcg atgccggaca cggcatcggc
     5581 ctcgacgcgg gcacccacgt ggcggccgag cattgcagcg aactcggttt cgttgggggt
     5641 gaggatgtcg gcgcgttcga gcatcgcgtg gtcgatcgcc acgttcgccg gcgccgggtt
     5701 gagcagggtg gtcgcaccgg cggcgcgacc ggcgtcgagc gccttggcca ccgcatccag
     5761 cggcgattcc agctgcgcca ccagcacgtc gcccggctgc aggccggcca gtgcctcggc
     5821 gacgaacgcc ggctgcaacg ccgcattggc gcccgcaccg atgacgatgc tgttgcggcc
     5881 ctgcgcatcc acgtagatgc cggcagtgcc ggtcggcgcc tgcacgacca cgtcgcgcag
     5941 gtcgatgccg tcggccgtgc acaggacccg cgccagctgc gcgcccaggt cgtcgccgag
     6001 cgcgcacacg aagcaggtgc ctgcgcctgc gcggcgcgcg gccaccgcct ggttgaagcc
     6061 cttgccgccg gggccggtgg tgtagtcgcc gctcagggtg gcgcccgcct gcggcaacgc
     6121 cggcacccgc cagacgtggt cgacattgaa ggaacctgcg acgacgacgc gattcatgcg
     6181 atcacccgaa atagctcagc acgccggcga tggtcgcggt catgaaggtg gcgatggacc
     6241 cgcccagcac cgcgcgcagg ccgaaccggg ccaggtcgct gcgacgttcc ggggccagcc
     6301 cgccgatgcc gccgatctgg atcgcgatcg agctgaagtt ggcgaagccg caca
//
LOCUS       DAOOCX010000125         6349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11494_length_6349_cov_9.586749, whole genome shotgun sequence.
ACCESSION   DAOOCX010000125 DAOOCX010000000
VERSION     DAOOCX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11494_length_6349_cov_9.586749"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /gene="rpsU"
                     /locus_tag="PK399_10160"
     CDS             complement(<1..139)
                     /gene="rpsU"
                     /locus_tag="PK399_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPW13522.1"
                     /translation="MPSVKVRENEPFEFALRRFKRTCEKAGVLAETRKREFYEKPTQE
                     RK"
     gene            274..1299
                     /gene="tsaD"
                     /locus_tag="PK399_10165"
     CDS             274..1299
                     /gene="tsaD"
                     /locus_tag="PK399_10165"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPW13523.1"
                     /translation="MKVLGIESSCDETGVAVYDTALSGAAGLRAHAVYSQVALHAEYG
                     GVVPELASRDHVRKLLPLIRQTLAEADLSVADLDGVAYTAGPGLVGALLVGAGVARSL
                     AWALDLPAVGVHHMEGHLLAPLMEDDPPAAPFVALLVSGGHTQLVAVDAIGAYRLLGE
                     TLDDAAGEAFDKTAKMMGLPYPGGPQLAKLAESGTPGVFRFARPMTDRPGLDFSFSGL
                     KTQVLLAWRNSDQSDTTRADIARGFEDAVVDTLAIKCERALDAAGCDTLVVAGGVGAN
                     KRLRAKLTDMCAKRGGRACFPRPALCTDNGAMIAFAGALRLEAGQREDAAVHVAPRWD
                     MATLPAV"
     gene            1343..2617
                     /locus_tag="PK399_10170"
     CDS             1343..2617
                     /locus_tag="PK399_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPW13524.1"
                     /translation="MSEWIGRLQASLQPYPWAWAGIALCGLVVIAWLANWLTRHVLLR
                     GLHRLLRATTHREAGQDREVRLAVVPRLANAVPALVLYAGVGAIPDLPALVVTVVRAL
                     CQAFVVLTLALAVARALDVVNRAYERRPKAHEKPIKGYLQVAKIVVYVLAALSVFATL
                     IGARFVHVLTGLGAMTAVTLLIFQDTILSFVASVQIGSDGRVRLGDWIQMPSQNADGE
                     VTDIALHTVTVQNWDKTITTIPTKKLISDSFKNWRGMIESGGRRIKRSLHIDQKSVRF
                     LGGDEARLMGRFRLIERYWGEKQRELDAWNARLDPQVDAVNARRVTNLGTYRAYVERY
                     LRAHPAVNQEMTLIVRQLQPCETGIPLEVYCFTNDTRWAAYECIQSDIFDHLLAILPE
                     FGLSVFQASSDAPLRISLTDARTPHRVPGAAC"
     gene            2647..3009
                     /gene="folB"
                     /locus_tag="PK399_10175"
     CDS             2647..3009
                     /gene="folB"
                     /locus_tag="PK399_10175"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPW13525.1"
                     /translation="MTDIVFIQGLRIDGLIGVYAHERTARRPLVFDIEMRFDTRAAAA
                     SDQLADALDYHAVSMRLVEFAGESRFQLVETLAERCAALLLGEFGATGVRLKLAKPGA
                     FEGADTVGVVIERGSWDA"
     gene            3006..3722
                     /locus_tag="PK399_10180"
     CDS             3006..3722
                     /locus_tag="PK399_10180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW13526.1"
                     /translation="MSLVLVTGASRGIGRAIVARFLREGCKVVACARGEAGLADLKAA
                     FPAVDCLSCDMRDGAAVDALAHDVQARHGVVDLLVNNAGAYRPGQIATEDDDALLDML
                     QANLFGAYRLTRRVLPAMVKRRSGMILNVCSTASIAAYPNGGSYSIAKHALHGFSRNL
                     REEMKPHGVRVVALLPGATLTASWDGVPLPAERLMPAEDVAEMAWAAWTMSARTVVED
                     IVMRPQRGDISEADFGDVPA"
     gene            3719..4210
                     /locus_tag="PK399_10185"
     CDS             3719..4210
                     /locus_tag="PK399_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPW13527.1"
                     /translation="MNDVVTIRLAKENMKFSAAHFTIFSATERERLHGHNFRVEVDIE
                     AHMLGNGMCFDYGIYKDRVVALCRELNEWTILPTRSEHLRIEEDGAHVFALFDGTRIP
                     FLRADVLLLPIENATLEEFAAWFLGRLGGDREALRAHRIDAIEVRVFSGPGQSAGRRA
                     RFD"
     gene            4210..5397
                     /locus_tag="PK399_10190"
     CDS             4210..5397
                     /locus_tag="PK399_10190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010368204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPW13528.1"
                     /translation="MASTLPPPDAAAQSHSAHLGEVIQEQVIAAGGHVPFWKFMELAL
                     YAPGLGYYSAGAHKFGPGGDFTTAPERSPLFSACVADALTPVLQQLGPDAVFMEIGGG
                     SGAFAETCLAKLLANDALPARYAILEPSADLRERQEERLQSRLPPLLFELVEWLDGPI
                     QDPWNGVLFANEVIDALPTPRFTIRDGEVFEEYVSLDGEGRFLRTDRPADPMLAAAVR
                     HIERQLPEPFAEGYRSEAMAQLPYWIQAVMGAMQDGAMLFIDYGYARGEYYQPQRRDG
                     TLRAFRQHHVTGDVFAWPGLQDITASVDFTALAEAGSAAGFDFAGYCSQASFLIGNRL
                     QENLALAESRAADEVARHALRSQVKHLTLPSEMGERFQAIGFQRGVELGAAFLVGDLS
                     HRL"
     gene            5394..5804
                     /locus_tag="PK399_10195"
     CDS             5394..5804
                     /locus_tag="PK399_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPW13529.1"
                     /translation="MSASPRRFGRSFKPFKRPWLWAGLWILAIACVVVGSLLPSSDLP
                     SVKVNDKVEHFVAYAVLSAGAVQLFARRLSWGFVCVLLVLMGIGLEVLQAQMGLGRML
                     DRNDALANTIGVLIGLATAMTPLRDVLLRFDRRL"
     gene            5801..>6349
                     /locus_tag="PK399_10200"
     CDS             5801..>6349
                     /locus_tag="PK399_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HPW13530.1"
                     /translation="MSPPARSSRFTSVDVLRGWAVAAMLLVNYPGDWSHLYAPLEHAA
                     WHGCTPTDLIFPTFLFVVGVSIALGMSRGTPSPAGRRGLLGKAWMRALRLVAIGLLLH
                     ALAMWAYDSAAFRPWGVLQRIGLCFGAAATAALYLRPRAQWLCIVGLLLGYWALLFAG
                     GSLEPWQNIASRVDAWMLGAHAY"
BASE COUNT          932 a         2107 c         2217 g         1093 t
ORIGIN      
        1 gcttgcgctc ctgggtcggc ttctcgtaga actcgcgctt gcgggtctcg gcgagcacgc
       61 cggccttctc gcaggtgcgc ttgaagcggc gcagggcaaa ctcgaagggc tcgttttcgc
      121 ggactttgac gctcggcatc tttggaatct cggaatcttt tggaaagtcg ggccgggagg
      181 ccaggctctt gcggagggac ccgcacacgc cggggcagtc cgggcgagcc gcgcatgata
      241 gcagcgggcc tctggccggt gcaagatagg cccatgaaag tcctcggcat cgaatcctcc
      301 tgcgacgaaa ccggcgtggc cgtgtacgac accgcgctgt ctggcgcggc cggcctgcgc
      361 gcccacgcgg tctacagcca ggtcgccctg cacgccgagt acggcggcgt ggtgccggaa
      421 ctggccagcc gcgaccacgt gcgcaagctg ctgccgttga tccgccagac cctggcggag
      481 gccgacctct cggtcgccga cctcgacggc gtggcctata cggccggtcc cggcctggtc
      541 ggggccctgc tggtcggcgc cggtgtggcg cgctcgctgg cctgggcgct ggacctgccg
      601 gcggttggcg tccatcacat ggaaggccat ctgctggccc cgctgatgga ggacgacccg
      661 cccgcggcgc cattcgtggc cctgctggtg tccggcgggc atacccaact ggtggcggtc
      721 gatgccatcg gcgcgtaccg gctgctgggc gaaaccctgg acgacgccgc cggtgaggcc
      781 ttcgacaaga ccgcgaagat gatgggcctg ccgtatcccg gcgggccgca gttggcgaag
      841 ctggcagaga gtggcacgcc cggcgtattc aggttcgccc ggccgatgac cgatcgtccg
      901 ggactcgatt tcagtttcag cgggctgaag acccaggtcc tgctggcctg gcgaaacagc
      961 gaccagtccg acacgacccg cgccgacatc gcccgtggat tcgaggatgc ggtggtcgac
     1021 accctggcga tcaagtgcga gcgcgcgctg gatgcggccg gctgcgacac cctggtggtc
     1081 gccggtggcg tgggcgcgaa caagcggttg cgcgcgaagc tcaccgacat gtgcgccaaa
     1141 cgcggcggcc gcgcctgctt cccgcgcccg gcgctgtgca ccgacaacgg cgcgatgatc
     1201 gccttcgccg gcgcgctgcg gctcgaagcc ggccagcgcg aggacgccgc ggtgcacgtg
     1261 gccccgcgtt gggacatggc gaccttgccg gcggtgtgat cgactaacgg gctgctgaca
     1321 tcgtccccgt tatcctgacc agatgagcga atggatcggg cgtctgcagg cgtcattgca
     1381 gccgtatccc tgggcttggg ccggtatcgc cctctgcgga ttggtcgtga tcgcatggtt
     1441 ggccaactgg ctcacccggc acgtgttgct gcgggggctg catcggctgc ttcgcgccac
     1501 cacccaccgc gaggccggcc aagaccgcga ggtgcggctc gcggtggttc cccggctggc
     1561 caatgccgtg ccggccctgg tgctgtatgc gggcgttggc gcgatccccg acctgccggc
     1621 actggtcgtg acggtagtac gcgcgctgtg ccaggcattc gtcgtgttga ccctcgcgtt
     1681 ggcggtcgcg cgcgcgctgg acgtggtcaa ccgcgcctac gagcgcaggc cgaaggcgca
     1741 tgaaaagccg atcaagggct acctgcaagt cgccaagatc gtggtgtatg tgctcgcggc
     1801 cctctcggta ttcgccaccc tgatcggtgc cagattcgtc catgtcctga ccggcttggg
     1861 cgcgatgacc gccgtcaccc tgctgatttt ccaggacacg atcctgtcgt tcgtggccag
     1921 cgtgcagatc ggcagcgacg ggcgggtccg gctgggagac tggatccaga tgccgagcca
     1981 aaacgccgac ggcgaagtca ccgacatcgc cctgcacacg gtcaccgtgc agaactggga
     2041 caagacgatc accacgatcc cgaccaagaa gctgatcagc gactcgttca agaactggcg
     2101 cgggatgatc gagtcgggtg gccgcaggat caagcgttcg ctgcacatcg accagaaaag
     2161 cgtgcgtttc ctcggcggag acgaggcgcg cctgatgggt cgcttccgcc tgatcgagag
     2221 gtactggggc gagaagcagc gcgagctcga tgcctggaac gcgcgtttag acccgcaggt
     2281 cgatgcggtc aacgcccgcc gcgtcaccaa cctgggcacg taccgtgcct acgtggaacg
     2341 ctacctgcgc gcccatccgg cggtgaacca ggagatgaca ctgatcgtgc gccagttgca
     2401 gccatgcgaa accggcatcc cgctggaggt gtactgcttc accaacgaca cccgttgggc
     2461 ggcatacgaa tgcatccagt ccgacatctt cgaccacctg ctggcgatcc tgcccgagtt
     2521 cgggctgtcg gtgttccagg cgtccagcga tgcgccgctg cgcatttcgt tgaccgatgc
     2581 ccggaccccg caccgggttc cgggcgctgc ctgctgagcg accttcgctg caggtatcct
     2641 tccgcgatga ccgacatcgt cttcatccag ggccttcgca tcgatggact gatcggcgtg
     2701 tacgcgcacg agcgcaccgc ccgcaggcca ctggtgttcg atatcgaaat gcgcttcgac
     2761 acgcgcgctg ccgcggccag cgaccagttg gccgatgcgc tggattacca cgcggtatcg
     2821 atgcggctgg tggaattcgc cggcgaaagc cgtttccagc tggtcgagac gctggccgaa
     2881 cgctgcgcgg cgttgttgct tggcgaattc ggcgcgaccg gcgtgcgcct gaagctggcc
     2941 aagccgggtg ccttcgaagg cgccgatacc gttggcgtgg tgatcgagcg cgggagctgg
     3001 gacgcatgag cctggtgctg gtcaccggcg cctcgcgcgg gatcggccgc gccatcgtgg
     3061 cgcgcttcct gcgcgagggc tgcaaggtcg tcgcctgcgc gcgcggcgag gccggcctgg
     3121 ccgacctgaa ggccgcgttc cccgccgtcg attgcctgtc ctgcgacatg cgcgatgggg
     3181 ccgcggtcga tgccctcgct catgacgtgc aggcccggca tggcgtggtc gacctgctgg
     3241 tcaacaacgc cggcgcgtat cggcccgggc agatcgcgac ggaagacgat gatgcgctgc
     3301 tcgacatgct gcaggccaac ctgttcggcg cctaccggct gacccggcgc gtgctgccgg
     3361 ccatggtcaa gcgccgcagc gggatgatcc tcaacgtgtg cagcacggcg tcgatcgccg
     3421 cgtatccgaa cggcggctcc tacagcatcg ccaagcatgc gttgcacggg ttttcgcgca
     3481 acctgcgtga agagatgaag ccgcatggcg tgcgcgtggt cgcgctgttg ccgggggcca
     3541 cgctgacggc ctcctgggac ggcgtgcccc tgccggcgga gcggttgatg ccggccgagg
     3601 atgtcgcgga gatggcatgg gcggcgtgga cgatgtcggc gcgcaccgtg gtcgaggaca
     3661 tcgtcatgcg cccgcagcgc ggcgatatca gcgaggcgga cttcggggac gtgcccgcat
     3721 gaacgacgtg gtgacgatcc gcctggccaa ggagaacatg aagttctccg ccgcacactt
     3781 cacgatcttc agcgcaaccg aacgcgaacg cctgcacggc cacaacttcc gcgtggaggt
     3841 cgacatcgag gcacacatgc tcggcaatgg catgtgcttc gactacggca tctacaagga
     3901 tcgcgtggtc gcgctgtgcc gcgaactcaa cgaatggacg atcctgccga cgcgatcgga
     3961 gcacctgcgc atcgaggaag acggcgcgca tgtgttcgcg ttgttcgacg gcacccgcat
     4021 cccgttcctg cgcgccgacg tgctcctgct gccgatcgag aacgccacgc tggaggaatt
     4081 cgccgcgtgg ttcctcggcc gtcttggcgg ggatcgcgag gcattgcgcg cgcaccgcat
     4141 cgacgcgatc gaggtgcggg tgttctccgg cccgggccag agcgcgggcc gccgcgccag
     4201 gttcgactga tggcctcgac cctgccgccg ccggacgccg ccgcgcagtc gcacagcgcg
     4261 cacctgggcg aggtgatcca ggagcaggtc atcgctgccg gcggccacgt tccgttctgg
     4321 aaattcatgg agctggcgct gtatgcgccg ggcctgggct actacagcgc cggcgcgcac
     4381 aagttcggtc ccggcggcga cttcaccacc gcgccggaac gctcgccgtt gttttccgcg
     4441 tgcgtcgccg atgcgctgac gccggtgctg cagcaactcg gtcccgatgc ggtgttcatg
     4501 gagatcggcg gcggcagcgg cgccttcgcc gaaacctgcc tggcgaagct gctcgccaac
     4561 gatgccctgc cggcgcgcta cgcgatcctc gaacccagcg ccgacctgcg cgaacgccag
     4621 gaggaacgcc tgcaatcgcg cctgccgcca ctgctgttcg aattggtgga atggctggac
     4681 gggccgatcc aggacccatg gaatggcgtg ctgttcgcca acgaggtgat cgacgcattg
     4741 ccgacgccgc gcttcaccat ccgcgatggc gaagtgttcg aggaatacgt gtcactggat
     4801 ggcgagggcc gcttcctgcg caccgaccgg ccggccgatc cgatgctcgc cgccgcggtg
     4861 cgccacatcg aacggcagct gccggagccg ttcgccgaag gctatcgcag cgaggcgatg
     4921 gcgcagttgc cgtactggat ccaggcggtg atgggcgcga tgcaggacgg cgcgatgctg
     4981 ttcatcgact atggctacgc gcgcggcgag tactaccagc cgcagcgccg cgacggcacc
     5041 ctgcgcgcgt tccgccagca ccatgtcacc ggcgacgtgt tcgcctggcc gggcctgcag
     5101 gacatcaccg cctcggtgga tttcaccgcg ctggcggagg ccggcagtgc tgccggtttc
     5161 gacttcgccg gctactgctc gcaggccagc ttcctgatcg gcaaccgcct gcaggagaac
     5221 ctggcgctgg cggaatcgcg cgctgcggat gaggtcgccc gccatgcctt gcgcagccag
     5281 gtgaagcacc tgaccctgcc cagcgagatg ggcgagcgct tccaggccat cggtttccag
     5341 cgtggcgtcg aactgggcgc ggccttcctg gtcggcgacc tgagccatcg cctgtgagcg
     5401 catcgccacg gcgcttcggc agatcgttca agccgttcaa gcggccatgg ttgtgggcgg
     5461 gcttgtggat cctggcgatc gcctgcgtcg tggtcggttc cttgctgcca agcagcgacc
     5521 tgccgtcggt caaggtcaac gacaaggtcg agcatttcgt cgcgtacgcc gtgttgtccg
     5581 cgggtgcggt gcagttgttc gcgcggcggt tgtcgtgggg cttcgtctgc gtgctgctgg
     5641 tgctgatggg catcggcctg gaagtcctgc aggcgcagat ggggctgggg cgcatgctcg
     5701 atcgcaacga cgccctggcc aataccatcg gcgtgctgat cggcctggcc accgcgatga
     5761 cgcccttgcg cgacgtgttg ctgcgcttcg accggcgcct gtgagtccac ccgcgcgatc
     5821 ttcgcgattc acctcggtcg atgtgctgcg cggctgggcg gtcgcggcga tgctgctggt
     5881 caactatccc ggcgattgga gccacctcta cgcgccgctc gagcatgcgg cctggcacgg
     5941 ctgcacgccg accgacctga tcttcccgac cttcctgttc gtggtgggcg tctcgatcgc
     6001 actcggcatg tcgcgcggca cgccatcgcc agcagggcga cgcgggttgc tcggcaaggc
     6061 gtggatgcgc gcgctgcgtc tggtcgcgat cggcctgctg ctgcatgcgc tggcgatgtg
     6121 ggcctacgac agcgccgcgt tccggccgtg gggcgtgctt cagcggatcg gcctgtgttt
     6181 cggcgcggcc gccaccgcgg cgttgtacct gcgcccgcgc gcgcaatggc tgtgcatcgt
     6241 cggcctgttg ctgggttact gggcgctgct gttcgccggc ggcagcctgg aaccgtggca
     6301 gaacatcgcc agccgggtgg atgcgtggat gcttggcgcg catgcctac
//
LOCUS       DAOOCX010000126         6337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11530_length_6337_cov_7.551576, whole genome shotgun sequence.
ACCESSION   DAOOCX010000126 DAOOCX010000000
VERSION     DAOOCX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11530_length_6337_cov_7.551576"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PK399_10205"
     CDS             <1..479
                     /locus_tag="PK399_10205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW13531.1"
                     /translation="LNLFCYTGVATVQAAVQGATQTTSVDLSATYLEWLADNLRGNGV
                     GGTRHRIAQADALKWLEADRGSYDVVFCDPPTFSNSKRADDFDVQRDHVRLLRAGVAR
                     LAPGGVLYFSNNFRRFKLDEAALGAFARVEDISASTIPPDFARNARIHRAWRITAA"
     gene            complement(538..1254)
                     /gene="aqpZ"
                     /locus_tag="PK399_10210"
     CDS             complement(538..1254)
                     /gene="aqpZ"
                     /locus_tag="PK399_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HPW13532.1"
                     /translation="MAMMKRLGAELIGTFWLVLGGCGSAVLAANFGGDGNPLGIGLLG
                     VSLAFGLTVLTGAYALGHISGGHFNPAVSFGLWAGGRFAAKDLLPYIVAQTLGAILAA
                     WILFQIASGTAGFAIDGNAAGAFATNGFDASSPGGYGMTAAFLCEVVMTAMFLVIILG
                     ATHKNAAAGFAPIAIGLGLTLIHLISIPVTNTSVNPARSTGVALLVGGTPLSQLWLFW
                     AAPIIGGVLGGVLYKWLGKD"
     gene            complement(1346..2437)
                     /locus_tag="PK399_10215"
     CDS             complement(1346..2437)
                     /locus_tag="PK399_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13533.1"
                     /translation="MALTLGTTGMDSKTKAEVQAAFKAANAETGNLWTLVDGDEADYI
                     VIDMDSLYGPMSWLRLHAAGRKVVGLTSVDRNQTDYRLPRPISANDFSVLLSEIAVDA
                     PAEAAAAKAAAENPEPAAARVEAAPTPAPAQPLPAAEPEPQEEPPQEPEPIRAPEPEP
                     EPEPTPEPVIEVPEDRGLARWLLAGGLDRRVRLQRGDGPVLLVDPRTRVWHGPATLKP
                     LLAYFEGTLQLEDFATPDAAAWESEAAAIGAAQPLSRLQWLGGLLAGHGALLPGLDPD
                     GKFRLLKWPQTEREYPKHFRIATAMMKGPATVAEVADASGVAKEDVADFVNANLATGY
                     AESTTDAPPEPEAPAPKPAGGLFGRMRGR"
     assembly_gap    2622..2721
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2731..3411)
                     /locus_tag="PK399_10220"
     CDS             complement(2731..3411)
                     /locus_tag="PK399_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPW13534.1"
                     /translation="MGTASATLRPVHAGTRSAPGHAEGIPSPEFLHGTSASPAPGLRL
                     LGLDAHGRALDWMSWQDATCLYARGAVAWTLGEPCLQVHGGINRASGERSIMHLHPII
                     AARGHARPHALAPTPALTNPALFARDQQLCLYCGNAYPRSTLTRDHVQPVSKGGRDIW
                     ENVVTACYHCNSRKGNRTPQQAGMPLLAVPYRPSWIEHLILSNRNILADQMAFLKSQL
                     PKKSPRFA"
     gene            complement(3516..5309)
                     /locus_tag="PK399_10225"
     CDS             complement(3516..5309)
                     /locus_tag="PK399_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPW13535.1"
                     /translation="MSTYKAPLDDLRFALYDVLGAEALFARLGQADVNRELVDAVLDE
                     AARFSETVLAPLNKVGDEIGCTYDKATGDVTAPPGFKQAFDQFVEGGWTGLTNAPEHG
                     GQGMPAVVGAVLTEMVDAANLAWGNFPMLSHGAVKALETYGEDWQKKVFLEPLVAGTW
                     TGTMCLTEPHCGTDLGLLKTRAEPASDDKYSITGTKIFITAGEHDLVPNIVHLVLAKL
                     PDAPPGAKGISLFVVPKFKVGRDGTVGERNALRCGAIEHKMGIHGSATCVMNFDGAEG
                     YLVGAPHKGLQAMFVMMNSARLGVGVQGLGLSERAYQNALRYARERLQSRSLSGPKLP
                     EKPADPIIVHPDVRRMLLSQKALVEGGRLLSLHAYSQLDIAEKSQDAAERERADILAG
                     FLTPISKACLTEWSIENTYNAVQCFGGHGYIAEHGIEQLARDARITTLYEGTTGIQSL
                     DLIGRKTAATQGAGLKLFLAEVEAFAKQHEGDEALAEFIAPLRAKAAEWGKLTMDVLQ
                     RAASNPEELGAASTDYLFYSGYVVLAYWWARSVAAANVSGRPQAFKDAKRDTARFYFA
                     RLLPRTLAHKAAIESGAAPLMSMPADAFGAE"
     gene            complement(5414..5884)
                     /locus_tag="PK399_10230"
     CDS             complement(5414..5884)
                     /locus_tag="PK399_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005990550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13536.1"
                     /translation="MKLRHVLIATTVLALVACGGGPVKRVSEPAAGIQQLSVRADGRW
                     DVVVRLDNFSSVPMRFADANLDIAFDNGAAAKLKAQPGISIGPESADVFTVTLTPLPA
                     GRARLANALADGQGLNYTLGGTVEAGPEEGKPRSYKIERKSALAPVPGLPGVLR"
     gene            complement(5940..6128)
                     /locus_tag="PK399_10235"
     CDS             complement(5940..6128)
                     /locus_tag="PK399_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13537.1"
                     /translation="MDIHDIALRLYAELVSANRVELVSEAARIELGREAYRYAEAFIA
                     AKDIYIRELPVANVEAGY"
     gene            complement(6257..6332)
                     /locus_tag="PK399_10240"
     tRNA            complement(6257..6332)
                     /locus_tag="PK399_10240"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6297..6299),aa:Phe,seq:gaa)
BASE COUNT          951 a         2081 c         2167 g         1038 t
ORIGIN      
        1 tcctcaacct gttctgctac accggcgtgg ccaccgtgca ggcggcggta cagggcgcga
       61 cgcagaccac cagcgtcgac ctgtcggcga cctacctgga atggctggca gacaacctgc
      121 gcggcaacgg ggtgggtggc acccgccacc gcatcgccca ggccgacgcc ttgaagtggc
      181 tggaagcgga ccgcggcagt tacgacgtgg tgttctgcga tccacccacg ttctccaatt
      241 ccaagcgtgc cgacgacttc gacgtgcagc gcgaccacgt gcgcctgctg cgcgcggggg
      301 ttgcgcggtt ggcaccgggt ggcgttctgt atttctccaa caatttccgc cgcttcaagc
      361 tcgatgaagc cgcgctcggc gccttcgccc gggtcgagga catcagcgct tcgaccatcc
      421 cgccggactt cgcccgcaat gcgcgcatcc accgcgcctg gcggatcacc gcggcctgac
      481 cgcaacgaag aagggccgca tcgctgcggc ccttcgcatg tcacgcagcg gcggggctca
      541 gtccttcccc agccacttgt agagcacgcc gcccagcacg ccgccgatga tcggtgcggc
      601 ccagaacagc cacagttgcg agagcggggt gccgcccacc agcagcgcca cgccggtcga
      661 acgcgccggg ttcaccgagg tgttggtgac cgggatgctg atcaggtgga tcagggtcag
      721 gcccaggccg atcgcgatgg gtgcgaaacc cgccgccgcg ttcttgtggg ttgcgccgag
      781 gatgatcacc aggaacatcg cggtcatgac gacttcgcac aggaaggcgg cggtcatccc
      841 gtagccgccc ggcgaggacg catcgaagcc gttggtcgcg aacgcgcccg cggcgttgcc
      901 gtcgatggcg aagccggcgg tgccgctggc gatctggaac aggatccagg ccgccaggat
      961 cgccccgagt gtttgcgcca cgatgtaggg cagcaggtcc ttcgcggcga agcgtccgcc
     1021 ggcccacagg ccgaagctca ccgccgggtt gaagtggcct ccggaaatgt ggcccagcgc
     1081 gtaagcgccg gtcagtacgg tcaggccgaa cgccagcgac acgccgagca atccgatccc
     1141 cagcggattg ccgtcgccgc cgaagttggc cgccagcacg gcgcttccgc agccgcccag
     1201 caccagccag aacgtgccga tgagttcggc acccagtcgt ttcatcatcg cgatctcccc
     1261 atcgagcgac gcgccagtcg cgcgcgcctt cacattagcc gaagcgcatg cggctggcgt
     1321 gggaaatgcg ttaaaccgga ctggctcagc ggccgcgcat gcgcccgaac agaccgcccg
     1381 ccggcttggg tgccggtgcc tcgggctccg gtggcgcgtc cgtggtggat tcggcatagc
     1441 cggtcgccag gttggcattg acgaaatcgg cgacgtcttc cttcgccacg ccgctggcgt
     1501 cggcgacctc ggcaacggtc gcggggccct tcatcatcgc ggtggcgatg cggaagtgct
     1561 tggggtattc gcgttcggtc tgcggccact tcagcaggcg gaacttgccg tccggatcca
     1621 gccccggcag cagcgcgcca tgcccggcca acaggccgcc cagccattgc aggcgggaca
     1681 gcggttgcgc ggcgccgatc gcggcggcct cggattccca cgccgcggca tcgggggtgg
     1741 cgaaatcctc cagttgcaag gtgccttcga agtaggccaa cagcggcttg agcgtggcag
     1801 gtccgtgcca gacgcgggtg cgcggatcga ccaggagcac cgggccatca ccgcgctgca
     1861 gccgaacacg gcgatccagg ccgccggcca gcagccagcg cgccaggccg cgatcctcgg
     1921 gaacctcgat gacaggttcg ggcgtgggtt ccggctccgg ctctggctcg ggcgcacgga
     1981 tcggttccgg ctcctgcggc ggttcctctt gcggctccgg ctccgcagcg ggaagcggct
     2041 gcgcaggtgc tggcgtcggc gcggcttcca cccttgctgc ggccggctcg gggttttccg
     2101 ccgcggcctt cgcagcggca gcttcggccg gtgcatccac cgcgatctcg ctgagcagga
     2161 cgctgaagtc gttggcgctg atcggccgcg gcaggcggta atcggtctgg ttgcggtcga
     2221 ccgaggtgag gcccacgacc ttgcgtcccg ccgcatgcag gcgcagccag ctcatcggcc
     2281 cgtacaggct gtccatgtcg atgacgatgt agtcggcttc gtcgccgtcg acgagggtcc
     2341 agagattgcc ggtttccgca ttggccgcct tgaaggcggc ctgcacctcg gccttggtct
     2401 tgctgtccat gccggtggtg cccagggtca gtgccatggc ggtgatccgc tgcggaaaac
     2461 atggagtgtt tgccatgcat gcagccggcg tcaacggatg cgctgcacgg ttccggtact
     2521 gcaggagcag gcaagtcggg agtcccccat taaggtggcc gatggatcag gcgaagcgcg
     2581 gcgacttttt cggcaactgc gatttcagga acgccatctg gnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nccgccgcgg tcaggcgaag cgcggcgact tcttcggcaa
     2761 ctgcgatttc aggaacgcca tctggtcggc gaggatgttc cgattcgaca ggatcaggtg
     2821 ctcgatccag ctggggcggt acggaaccgc gagcagcggc atcccggctt gctgcggcgt
     2881 cctgttgccc ttgcgcgaat tgcagtggta gcaggcggta accacgtttt cccagatgtc
     2941 gcggccgccc ttggacaccg gctgcacgtg gtcgcgggtg agggtggagc ggggataggc
     3001 gttaccgcag tagaggcaca gttgctggtc gcgggcgaac agcgccgggt tggtgagggc
     3061 cggggtcggg gccagcgcat gtgggcgcgc gtgcccgcgc gccgcgatga tcgggtgcag
     3121 gtgcatgatg ctgcgctcgc cgctggcgcg gttgatgccg ccatgcactt gcaggcaggg
     3181 ctcgcccagg gtccacgcca cggcgccgcg cgcatacagg caggtcgcgt cctgccagct
     3241 catccagtcg agcgcgcggc cgtgcgcatc caggcccaac aggcgaaggc ccggggcggg
     3301 cgaagcggat gtgccgtgga ggaattcggg ggacgggatc ccttccgcgt gacctggcgc
     3361 ggatcgggtt ccggcatgga ccgggcgtag cgttgcgcta gcggttccca tggggtcatc
     3421 agcttatacg cctccgatga ccatttgggt acaggcatgc cggcgtcgat gccgcatgcc
     3481 atcgcgcccg gcgcatgccg ggcgcgatgt gaccgtcact cggccccgaa ggcgtccgcc
     3541 ggcatggaca tcagcggtgc cgcgccgctt tcgatcgccg ccttgtgggc cagcgtgcgt
     3601 ggcaacaggc gcgcgaagta gaagcgggcg gtgtcgcgct tggcatcctt gaacgcctgc
     3661 gggcggccgg agacattggc ggcagcgacg ctgcgcgccc accagtaggc cagcaccacg
     3721 tagccggaat agaacaggta gtcggtgctg gcggccccga gttcctccgg attgctggca
     3781 gcgcgctgca gcacgtccat ggtgagcttg ccccactcgg ccgccttggc gcgcagcggc
     3841 gcgatgaact cggccagcgc ctcatcgcct tcgtgctgct tcgcgaaggc ttccacctcg
     3901 gccaggaaca acttcagccc ggcgccctgg gtggcggcgg tcttgcggcc gatcaggtcc
     3961 agcgactgga tgccggtggt gccttcgtac agcgtggtga tgcgcgcatc gcgcgccagc
     4021 tgctcgatgc cgtgctcggc gatgtagccg tggccaccga agcactgcac cgcgttgtag
     4081 gtgttctcga tgctccactc ggtcaggcag gccttggaga tcggggtcag gaagccggcc
     4141 aggatgtccg cgcgctcgcg ctcggcggcg tcctgcgact tctcggcgat gtcgagctgc
     4201 gagtaggcat gcaggctgag caggcggccg ccttcgacca gcgccttctg cgacagcagc
     4261 atgcggcgca cgtcggggtg gacgatgatc gggtcagccg gtttttccgg cagcttcggg
     4321 ccggacagcg aacgcgactg caaccgttcg cgggcgtagc gcaacgcgtt ctggtaggcg
     4381 cgctccgaca ggcccaggcc ctgcacgcca acgccgagcc gcgccgagtt catcatcacg
     4441 aacatcgcct gcaggccctt gtgcggcgcg ccgaccaggt agccctcggc gccgtcgaag
     4501 ttcatgacgc aggtggccga gccgtggatg cccatcttgt gttcgatcgc gccgcagcgg
     4561 agcgcattgc gctcgcccac cgtgccgtcg cggccgacct tgaacttcgg caccacgaac
     4621 agcgaaatgc ccttggcgcc gggcggcgcg tcgggcagct tggccagcac caggtggacg
     4681 atgttcggca ccaggtcgtg ctcgccagcg gtgatgaaga tcttggtgcc ggtgatcgag
     4741 tacttgtcgt cggaggccgg ttccgcgcgg gttttcagca ggcccaggtc ggtgccgcaa
     4801 tgcggctcgg tcaggcacat cgtcccggtc caggtgcccg ccaccagcgg ctccaggaac
     4861 accttcttct gccagtcctc gccgtaggtc tccagcgcct tgaccgcgcc gtgcgacagc
     4921 atcgggaagt tgccccaggc caggttggcg gcatcgacca tctcggtcag gaccgcgccg
     4981 accaccgccg gcatgccttg gccgccgtgc tccggcgcat tggtcaggcc ggtccagccg
     5041 ccttccacga actggtcgaa ggcttgcttg aagccgggcg gcgctgtgac gtcgccggtg
     5101 gccttgtcgt aggtacagcc gatctcgtcg ccgaccttgt tcagcggggc cagcacggtt
     5161 tcggaaaagc gtgcggcttc gtccagcacg gcatccacca gttcgcggtt gacgtcggcc
     5221 tggcccagtc gggcaaacag cgcctccgcg ccaagcacgt cgtagagggc gaaacgcaaa
     5281 tcgtccagcg gggccttgta ggtgctcatg cggcgttacc agtcatgacg ggccgccggt
     5341 cggcccgtgg aaacgcaagc atactacggc gtatggtttg tcggaatcct tccggaaatc
     5401 gcgctccgca tcctcagcgc agtacgcccg gcaggccggg caccggcgcc agcgcgctct
     5461 tgcgctcgat cttgtaactg cgcggcttgc cttcttccgg gccggcttcc accgtgccgc
     5521 ccagcgtgta attcaagccc tggccatccg cgagggcatt ggccagccgc gcgcgacccg
     5581 cgggcagcgg cgtcagcgtg accgtgaaca catcggccga ttccgggccg atgctgatgc
     5641 ccggctgcgc cttcagtttc gccgccgcgc cgttgtcgaa ggcgatgtcc aagttggcgt
     5701 cggcgaagcg catcgggacg ctgctgaaat tgtccagccg caccaccacg tcccagcggc
     5761 catcggcacg cacgctcagt tgctggatgc cggcagcggg ttcggagacg cgcttcaccg
     5821 gcccgccacc acaggccacg agtgcgagga cggtggtggc gatcaggacg tggcgcagtt
     5881 tcatggaggc ttcctcggcg gtttgcccga ggatagccgc aatcccgagc ggcatcgcgt
     5941 cagtagccgg cctcgacgtt ggcaaccggc aactcgcgga tatagatgtc cttggccgcg
     6001 atgaaggctt ccgcgtagcg gtaggcctcg cggccgagtt cgatgcgtgc agcttcgctc
     6061 accagctcga cgcggttggc ggacaccaat tcggcgtaca acctcagggc gatgtcgtgg
     6121 atgtccatgc gggattcctc gggagatggc ggcgaccata cacccggcct gcaagcggcg
     6181 cgatatcgag gactgtcgtg ggccaggaaa gcgaaaaccc cgcgcgtggc ggggtttgcg
     6241 gcatcgtctt ccggagtggt ggccggggag ggaatcgaac ccccgacacg gggattttca
     6301 atcccctgct ctaccaactg agctacccgg ccagtgc
//
LOCUS       DAOOCX010000127         6271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11703_length_6271_cov_6.934363, whole genome shotgun sequence.
ACCESSION   DAOOCX010000127 DAOOCX010000000
VERSION     DAOOCX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11703_length_6271_cov_6.934363"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /gene="uvrA"
                     /locus_tag="PK399_10245"
     CDS             complement(<1..2071)
                     /gene="uvrA"
                     /locus_tag="PK399_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPW13538.1"
                     /translation="MALDTIRIRGARTHNLKNIDLDLPRDKLIVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLSVMDKPDIDHIEGLSPAISIEQKSTSHNPRSTVGT
                     ITEIHDYLRLLYARVGTPRCPDHHFPLEAQTVSQMVDQVLAMEGDEVLAAQRWMLLAP
                     VVRDRKGEQQQVFDQLRAQGYVRVRVDGVLHEIDAVPPLAPRQKHTIEAVIDRFKPRA
                     DIRQRLAESFETSLKLGEGMAIVQSMDAPEREPLLFSSKYSCPVCDYALPELEPRLFS
                     FNAPMGACPGCDGLGVAQFFDPSRVVGHPELSLAAGAMRGWDRRNAYYFSMITSLAKH
                     YEFDVDLPWQLLPDEIRAIVLHGSGKEAINLVYVGENGNKYQRKHAFEGIIPNLERRY
                     RETESAAVREELSKYISERPCPECGGARLNRSARNVFVADRPLHEIAVMPIDNCLAFF
                     HGLKLPGWRGEIAAKIVKEIGERLSFLVDVGLDYLTLERKADSLSGGEAQRIRLASQI
                     GAGLVGVMYVLDEPSIGLHQRDNERLLGTLTRLRDLGNTVIVVEHDEDAIRLADHVVD
                     IGPGAGVHGGEVVAQGTLPDILKAPRSITGQYLSGKKRIEVPAHRNPPNRKLMLKLKG
                     AHGNNLKNVDLEIPSGLFTAITGVSGSGKSTLINDTLYALAANEINGASHTPSPYREI
                     TGLDLFDK"
     gene            2404..2724
                     /gene="rplU"
                     /locus_tag="PK399_10250"
     CDS             2404..2724
                     /gene="rplU"
                     /locus_tag="PK399_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPW13539.1"
                     /translation="MYAVLVTGGKQYRVMQGETLRVEKLEAEAGSEIKFDNILMLGDA
                     DGIKLGDAIKGASIAAKVVGHGRADKVRIVKFRRRKHHRKQMGHRQHYTEIEITGIAG
                     GDKK"
     gene            2737..2997
                     /gene="rpmA"
                     /locus_tag="PK399_10255"
     CDS             2737..2997
                     /gene="rpmA"
                     /locus_tag="PK399_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPW13540.1"
                     /translation="MAHKKGVGSSRNGRDSNPKYLGVKIYGGQAIEAGNIIVRQRGTQ
                     FHPGMGVGLGRDHTLFALVDGKVEFTTKGPNKRRTVSIVAAE"
     gene            3100..4185
                     /gene="obgE"
                     /locus_tag="PK399_10260"
     CDS             3100..4185
                     /gene="obgE"
                     /locus_tag="PK399_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPW13541.1"
                     /translation="MKLVDEVEITVTAGNGGNGCIAFRREKFIPLGGPNGGDGGNGGD
                     VWVQADENLNTLVDFRHETRFKAQRGENGMGSQMYGKAGEDKTIVVPVGTVVHNVDTD
                     EIIGDLTEHGQRLLVARGGLGGLGNMHFKSSVNRTPRRATPGTEGETRGLRLELKLLA
                     DVGLLGFPNAGKSTFVRAVSAATPKVADYPFTTLYPNLGVVSVEPGRSFVIADIPGLI
                     EGAADGAGLGSLFLRHVQRTRLLLHLVDIAPMEGGIEATPAEQVRAIEHELRKYDPAM
                     LDKPRWLVLNKADLMFEDEAKAAAEAIVSELGWTQPWYLVSAIGREGTRPIMLAVQAF
                     FDRMREDELEAAKDAEAAAEASRPGNA"
     gene            complement(4250..4519)
                     /gene="rpsT"
                     /locus_tag="PK399_10265"
     CDS             complement(4250..4519)
                     /gene="rpsT"
                     /locus_tag="PK399_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPW13542.1"
                     /translation="MANIKSAKKRAKQTVVRNERNVAQRSMLRTAVKKVIKALDANDA
                     EAASAAFITAQPLLDRFAARGLIHKNKAARHKARLTARIKALKAA"
     gene            4657..6267
                     /gene="murJ"
                     /locus_tag="PK399_10270"
     CDS             4657..6267
                     /gene="murJ"
                     /locus_tag="PK399_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPW13543.1"
                     /translation="MSRLLRSTAVFSAMTFLSRVSGLVRDQVYAIVFGANPAMDVFIA
                     AFRIPNFMRRLSAEGSFSMAFVPVLAEYKEQHGPQAVRELVDRVAGTLLAGLLVLTAV
                     VLLAAPWLTQLIAPGFVPGSEKHALFIEMLRITFPYALFISLASLVGSILNSYQRFAI
                     PALSPVLLNLAMIAAALGLAPLMGGSVKALAWGVFAAGILQLVFQLPALAKLGLLPRP
                     RLDFAHAGVRKILKLMVPTLFGSSVVQFNLLFNTMAASFLIGGSMTWLYYSDRLLEFP
                     LGMFGVAIGTVILPHLSTRHAATDPDGFSKGLDWGFRLCLLIGLPACAGLILCAEPLM
                     ATLFQYRSFSALDAKMSSWSLIAQSTAVPAFLLVKVLAPAFYSRQDTRTPVKAAIVAV
                     LTNALCTVGLFAWLLHATPTGQAALQAAGGNWRQALAGIHGTHALLALAIAVAGWVNA
                     LQLAWLLRGAGVYRRQPGWGRFIGQLLAATLALAVVVLLFRHAWPDWGDWAWWERGWR
                     LAAMVGGGAVAYAGVLLALGMRPRDLRH"
BASE COUNT          988 a         2004 c         2182 g         1097 t
ORIGIN      
        1 ccttgtcgaa caggtcgagg ccggtgatct cgcgatacgg ggagggcgtg tgcgaggcgc
       61 cgttgatctc gttggcggcc agcgcataca gggtgtcgtt gatcagggtg gacttgccgg
      121 aacccgagac gccggtgatc gcggtgaaca ggcccgaggg aatctcgagg tcgacgttct
      181 tcaggttgtt gccgtgcgcg cccttcagct tgagcatcag cttgcggttg ggcgggttgc
      241 ggtgcgccgg cacctcgatc cgcttcttgc cggacaggta ttgcccggtg atcgagcgcg
      301 gtgctttcag gatgtcgggc aaggtgccct gcgccacgac ttcgccgcca tgcacgccgg
      361 cacccggtcc gatgtcgacc acatggtcgg ccaggcggat cgcgtcctcg tcgtgttcga
      421 cgacgatcac cgtgttgccc aggtcgcgca ggcgggtgag ggtaccgagc aggcgctcgt
      481 tgtcgcgctg gtgcaggccg atgctgggtt cgtctagcac gtacatcacc ccgaccaggc
      541 cggcaccgat ctgcgacgcc aggcggatgc gttgcgcctc gccgcccgac aggctgtcgg
      601 ccttgcgctc cagggtgagg tagtcgaggc ccacatcgac caggaagctc agccgctcgc
      661 cgatttcctt gacgatcttg gccgcgatct cgccgcgcca gcccggcagc ttgaggccgt
      721 ggaagaaggc gaggcagttg tcgatcggca tcaccgcgat ttcgtgcagc gggcggtcgg
      781 ccacgaacac gttgcgggcg gagcggttca ggcgcgcgcc gccgcattcg gggcaggggc
      841 gctcgctgat gtacttggac agttcctcgc gcaccgccgc ggattccgtc tcgcggtaac
      901 ggcgctccag gttcgggatg atgccctcga aggcgtgctt gcgctggtac ttgttgccgt
      961 tctcgcccac gtagaccagg ttgatcgcct ccttgccgct gccgtgcagg acgatcgcgc
     1021 ggatctcgtc cggcagcagc tgccagggca ggtcgacgtc gaattcgtag tgcttcgcga
     1081 gcgaggtgat catcgagaag taatacgcgt tgcggcggtc ccagccgcgc atcgcgccgg
     1141 ccgccagcga cagctccgga tggccgacca cgcgcgacgg atcgaagaac tgggccacgc
     1201 ccaggccgtc gcagcccggg caggcgccca tcggcgcatt gaacgagaac agccgcggtt
     1261 ccagttccgg caacgcgtag tcgcagaccg ggcaggagta cttcgacgag aacagcagcg
     1321 gctcgcgctc cggcgcgtcc atggactgca cgatcgccat gccttcgccc agcttcaggg
     1381 aggtctcgaa cgattcggcg aggcgctggc ggatgtccgc gcgcggcttg aagcggtcga
     1441 tcaccgcctc gatggtgtgt ttctggcgcg gcgccagcgg cggcaccgcg tcgatctcgt
     1501 ggagcacgcc gtccacgcgc acccgcacgt agccctgcgc gcgcaactgg tcgaacacct
     1561 gctgctgctc gcccttgcgg tcgcgcacca cgggcgccag cagcatccag cgctgcgccg
     1621 ccaggacctc gtccccttcc atcgccagca cctggtcgac catctggctg accgtctgcg
     1681 cttccagcgg gaagtggtgg tcggggcagc gcggcgtgcc cacgcgcgcg tacagcaggc
     1741 gcaggtagtc gtggatctcg gtgatcgtgc cgaccgtgga gcgcgggttg tggctggtgg
     1801 acttctgctc gatcgagatc gccgggctca acccttcgat gtggtcgatg tcgggcttgt
     1861 ccatcacgct gaggaattgc cgcgcgtagg ccgacagcga ctcgacgtag cggcgctggc
     1921 cttccgcata gatcgtgtcg aacgccagcg acgacttgcc ggaaccggac aggccggtga
     1981 tcacgatcag cttgtcgcgc ggcaggtcga ggtcgatgtt cttcaggttg tgcgtgcgag
     2041 caccgcggat gcggatggtg tccagcgcca tggatggggg atcctgggga aggggagcgg
     2101 gcgaggtgcg gctgccgtgc aacaggcaat cgggcagcgt accgagccgg tgatttgggg
     2161 gcaaatgacg ggattgccag cgtggttcac ggctgtctct gtcgctgcga ctcgaacacc
     2221 atgcccctcg gcacacgcca cctccgcatg ctcggggaag gggagagtgc ctgcagttga
     2281 ccgtaactcg ctgaatcccc tacaattccg ctcctgtctg ccctcgatgg caggcggcgt
     2341 gccgggaacc acgtccggca tgaccaagaa gaactacaga atccacagtc aggaaccacc
     2401 accatgtacg cagttttggt caccggcggt aagcaatacc gcgtgatgca gggcgaaacg
     2461 ctccgcgtcg agaagctcga agccgaagcc ggcagcgaga tcaagttcga caacatcctc
     2521 atgctcggcg acgccgacgg catcaagctg ggcgatgcga tcaagggggc gtcgatcgcc
     2581 gccaaggtcg tgggccacgg ccgcgccgac aaggtccgca tcgtcaagtt ccgccgccgc
     2641 aagcaccatc gcaagcagat gggccaccgg cagcactaca ccgaaatcga gatcaccggc
     2701 atcgccggtg gcgacaagaa gtaaggagac gcagtcatgg cacataaaaa gggcgtaggt
     2761 tcctcgcgca acggccgcga ctccaacccg aagtacctcg gcgtgaagat ctatggtggc
     2821 caggcgatcg aagccggcaa catcatcgtt cgccagcgcg gcacccagtt ccacccgggc
     2881 atgggcgtcg gccttggccg cgaccacacc ctgttcgcgc tggtcgatgg caaggtcgag
     2941 ttcacgacca agggcccgaa caagcgccgc acggtcagca tcgtcgccgc ggaataatcg
     3001 ccgccgacgc atcgcgaaga gccccgcttc ggcggggttt ttcgtttatg caggccctcg
     3061 ctcctcaccc gcttcaccga acacggcaca atcccccaca tgaaactcgt cgacgaagtt
     3121 gaaatcaccg tgaccgcagg caacggcggc aatggctgca tcgccttccg ccgcgagaag
     3181 ttcattccgc tgggcggtcc caatggcggc gacggcggca acggcggcga cgtctgggtg
     3241 caggccgacg agaacctcaa caccctggtc gacttccgcc atgaaacgcg cttcaaggcg
     3301 cagcgcggcg aaaacggcat gggcagccag atgtacggca aggccggcga ggacaagacc
     3361 atcgtcgtgc cggtcggcac cgtggtccac aacgtcgata ccgacgagat catcggcgac
     3421 ctgaccgagc acggccagcg cctgctggtg gcccgaggcg gcctgggtgg cctcggcaac
     3481 atgcatttca agagttcggt caaccgcacg ccgcgccggg ccacgccggg caccgagggc
     3541 gaaacccgcg gcctgcgcct ggaactgaag ctgctggccg acgtgggttt gctcgggttc
     3601 ccgaatgccg gcaagtccac cttcgtgcgc gcggtgtccg cggcgacgcc gaaggtcgct
     3661 gactatccgt tcaccacgct gtacccgaac ctgggcgtgg tcagcgtgga gcctgggcgc
     3721 agcttcgtga tcgccgacat ccccggcctg atcgagggcg cggccgatgg tgccggcctg
     3781 ggcagcctgt tcctgcggca tgtgcagcgc acccgcctgc tgctgcacct ggtcgacatc
     3841 gcgccgatgg aaggcgggat cgaggccacc ccggccgagc aggtccgcgc gatcgaacac
     3901 gagttgcgca agtacgaccc ggcgatgttg gacaagccgc gttggctggt gctgaacaag
     3961 gccgacctga tgttcgagga tgaggcgaag gccgcggccg aggcgatcgt ctccgaactc
     4021 ggctggaccc agccctggta tctggtctcg gcgatcgggc gcgaaggcac ccggccgatc
     4081 atgttggcgg tgcaggcgtt cttcgaccgc atgcgcgagg atgaactcga ggcggccaag
     4141 gatgcagagg ccgcggcgga agcatcgcgc cccggcaatg cctgaggtgc tggcccaggc
     4201 aacaaaaaac ccggccgagg ccgggttttt cgcgaaaggt gctgagggct caggcggcct
     4261 tcagtgcctt gatgcgcgcg gtcaggcgag ccttgtgacg ggcagccttg ttcttgtgga
     4321 tcaggccgcg ggcggcgaaa cgatcgagca gcggctgcgc ggtgatgaac gcggcggaag
     4381 cggcttcggc gtcgttggcg tcgagcgcct tgatcacctt cttgacggcg gtacgcagca
     4441 tggagcgctg ggcgacgttg cgttcgttgc gcacgacggt ctgcttggcg cgcttcttgg
     4501 cggacttgat attggccacg atgaaaatcc tgggaagtct gttggggctg agagaatcgg
     4561 ccgccggcag gactgcctac ggacgaaggg atcgggaaga ctcaaaatta tggacgattc
     4621 aatggcttgg gtcaacttgc ctgccaaggg ggcggcatga gtcgcttgct ccgctcgacc
     4681 gcggtgttca gcgcgatgac cttcctgtcc cgggtttccg ggctggtccg cgaccaggtg
     4741 tacgcgatcg tgttcggggc caacccggcg atggacgtgt tcatcgcggc cttccgcatc
     4801 cccaatttca tgcgccggct gtccgccgag ggctcgttct cgatggcctt cgtgccggtg
     4861 ttggccgagt acaaggaaca gcacggcccc caggcggtgc gcgagttggt cgaccgggtc
     4921 gcgggcaccc tgctggcggg gttgctggtg ctcaccgccg tggtcctgct ggcggcgccg
     4981 tggctgaccc agctcatcgc acccggcttc gtgccgggca gcgagaagca cgcgctgttc
     5041 atcgagatgc tgcggattac cttcccgtac gcgctgttca tctcgctggc ctcgctggtg
     5101 ggcagcatcc tcaacagcta ccagcgcttc gccatcccgg cgctgtcgcc ggtcttgctc
     5161 aacctggcga tgattgcggc ggcgcttggg ctggcgccgc tgatgggtgg ctcggtcaag
     5221 gcactggcct ggggcgtctt cgccgccggg atcctgcaac tggtgttcca gttgccggcg
     5281 ctggccaagc tgggcttgct gccacggcca aggctcgatt tcgcccacgc cggcgtgcgc
     5341 aagatcctca agctgatggt gccgaccctg ttcggctcgt cggtggtcca gttcaacctg
     5401 ctgttcaaca cgatggccgc gtcgttcctg atcggcggca gcatgacctg gctctactac
     5461 agcgaccgcc tgctggagtt cccgctgggg atgttcgggg tggcgatcgg caccgtgatc
     5521 ctgccgcacc tgtcgacccg gcacgccgcg accgaccccg acgggttttc gaaaggcctg
     5581 gactggggtt tccggctgtg cctgctgatc ggcttgccgg cctgtgccgg gctgatcctg
     5641 tgcgccgagc cactgatggc caccctgttc cagtaccgca gtttcagcgc gctcgacgcg
     5701 aagatgagca gctggagcct gatcgcgcag tccaccgcgg taccggcgtt cctgctggtc
     5761 aaggtgctgg cgccggcgtt ctattcccgg caggacacgc gcacgccggt gaaggccgcg
     5821 atcgtggcgg tgctgaccaa tgcgctgtgc acggtcggcc tgttcgcctg gctgttgcat
     5881 gccacgccga ccgggcaggc ggcgctgcag gccgcgggcg ggaattggcg gcaggcgctg
     5941 gccggcatcc atggcaccca tgcgctgctg gcgctggcga ttgccgttgc aggttgggtc
     6001 aacgcgctgc aacttgcctg gctgttgcgt ggcgcaggcg tctatcgtcg ccagcccggc
     6061 tgggggcgct tcattgggca gttgctggcc gccacgctgg cgctggcagt ggtcgtgctg
     6121 ctgttccgcc atgcgtggcc ggattggggc gactgggcgt ggtgggaacg tggctggcgg
     6181 ttggcggcga tggtcggcgg tggggccgtg gcctatgcgg gcgtgctgtt ggcgctgggc
     6241 atgcggccgc gcgacctgcg ccactgacgg t
//
LOCUS       DAOOCX010000128         6250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11761_length_6250_cov_7.172881, whole genome shotgun sequence.
ACCESSION   DAOOCX010000128 DAOOCX010000000
VERSION     DAOOCX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11761_length_6250_cov_7.172881"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PK399_10275"
     CDS             complement(<1..415)
                     /locus_tag="PK399_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW13544.1"
                     /translation="MHGNRDVLARLQQATKSYGKVRALDGLDLSLRAGQVTSLLGANG
                     AGKTTAIGLLLGLLRADSGSVELFGQDPRELAARRRIGVMLQTAGLPERLRVGELLAQ
                     ARSYYPDARSIADCVALAGLDGLLDRPYGKLSGGQQ"
     gene            616..1848
                     /locus_tag="PK399_10280"
     CDS             616..1848
                     /locus_tag="PK399_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW13545.1"
                     /translation="MNSSADLLKSLKIDRSAPPPPTSRKGLWIGLGVAAAVVLGAGGW
                     FLLGRDSAVQVETTSAVSLASGAGNASVLDASGYVVARRMATVSSKITGRVREVLIEE
                     GQRVEEGQVMATLDPIDADAQRDLSAAQLAAAQSQIVGTQAQLKEAEANAARLGALVG
                     QKLVSRAQFEQAVAQRDALRAQVATAQRNAQVARSSLAIASNGVDNTVVRAPFAGVVI
                     AKAAQPGEIVSPLSAGGGFTRTGIGTIVDMDSLEVEVDVGEAYIGRVKPKMPVEATLN
                     SYPDWKIPAEVIAIIPTADRGKATVKVRVALKARDGRIVPDMGVTVSFLEKAADATAE
                     PKRGVRVPARAIVQRDGGDAAFVVGAEDIVELRKVQVGGALGEDREVSSGLAPGETVV
                     IAPPATLADGDRIATKTE"
     gene            1917..2609
                     /locus_tag="PK399_10285"
     CDS             1917..2609
                     /locus_tag="PK399_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW13546.1"
                     /translation="MASLVSTRNLTKTYQRGPEKVEVLHGIDLDIAEGDFVALMGPSG
                     SGKTTLLNLIGGLDSPSGGTIEVAGQRIDQLGGGKLAQWRSQHVGYVFQFYNLMPALT
                     AQKNVELPLLLTRLSGAQRKRNAQVALQLVGLSDRVSHKPTELSGGQQQRVAIARAIV
                     SDPTLLICDEPTGDLDRQSAEDILGLLQTLNREHGKTIVMVTHDPKAAGYASHTLHLD
                     KGTLVGDHADAA"
     gene            2631..3797
                     /locus_tag="PK399_10290"
     CDS             2631..3797
                     /locus_tag="PK399_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPW13547.1"
                     /translation="MKYFHLIWAALFRRKTRTFLTLASILAAFLLFGMLDGIRTTFDQ
                     LGRNADGAQRLQTSSRLSFMDSLPLSLQSRLERVDNIEAVTHANWFGGVYQNPKNQLF
                     TFAVADNYLDLYPEMLVAPAEREAWMRTRTGILVGEAMMKRFGWKVGQKIPLQSNIFP
                     NSDGTLNWAFDIVGTIKAKDGKGGGFTDAVILMHYKYFEESTSYVDGDVGWYISRVAD
                     VRRSDAAAKAIDALSVNSQHETKTMNEQAAMAAQLKQVADIGMIVGSIMGAVFFTLVL
                     LTGNTMAQAVRERTSELAVLKTIGFTSASVLGMVLAESVLLVLIGGVLGLGLAAVLAP
                     IVGAASGGAINLPPIGWRSWSLGLALMVGIGLLVGALPAMRAMRLNIVDALAGR"
     gene            3811..5130
                     /locus_tag="PK399_10295"
     CDS             3811..5130
                     /locus_tag="PK399_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW13548.1"
                     /translation="MKKIKSFLRGLALLLVLAVILAAWIVLPWPALLGAAVLFGLWMA
                     FTRSGRQAASVTQVGISTLNQRIGASSVVVIGIAGVVGVLVALLAMGEGYRQTVSNSG
                     SEDTAIVLRGGSSAELGSVLSRDQISAIERAPQIARDAQGRPLASAELMVAATVPLKG
                     SKDDGNASLRGVSEMAYAVRPKLKLVAGRRFEPGRRELVVGTGAQRQFDGLEVGKQVR
                     LGNQPWTVVGVFDSQDATASEMWADAEVVAATYRRGSSRAALYAKLTSAKDFNAFKAT
                     LAADPRLQVEADTTLAYFGKQSEGLTKVLRIIGIVVGSIMAIGAVFGALNTMFATVAS
                     RAREIATLRAIGFRGIPVVVAVMLETMLLALLGGVLGGLVAWLLFNGHTASTLAGGMS
                     QLSFQFKVDPTLLWEGLKWALAIGFIGGLFPALRAASLPVTDALRSA"
     gene            5258..>6250
                     /locus_tag="PK399_10300"
     CDS             5258..>6250
                     /locus_tag="PK399_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPW13549.1"
                     /translation="MRRALLILCACLLLAGFAWPDAADMLGKRLVAPGGVSPILPTTR
                     IEASIARLPHRQGWVSTSAGVKLFWRAFDPGAYRLDYAYRPDGTQAGRMQFDLDAAAP
                     VRYPGAPRGTVVLLHGWMMDGGSLLPWSLQLAQAGYRTITLDLRNHGRSGAAPSGYGQ
                     REARDVADAITALRERGEVVGPLHLMGVSYGAATAIFSARELGAEVNNVIAIESFDNA
                     GGAIRDMVPHMLAKPPESMSQRISHRLLRMQLDDGALERAIASASDTLQLRLDRVDVG
                     AALAQVPSCVLLLHGREDQHVPVAHGRALAAAAPRAHYIEVAGEDHISLPMRLDL"
BASE COUNT         1001 a         2148 c         2138 g          963 t
ORIGIN      
        1 gctgctggcc gccggagagc ttgccgtagg gacgatccag caggccgtcc aggccagcca
       61 gcgccacgca atcggcgatg ctgcgcgcgt cggggtagta gctgcgcgcc tgcgccagca
      121 actcgcccac gcgaagccgc tccggcaggc cggcggtctg cagcatcacg ccgatccggc
      181 gacgcgcggc gagttcgcgc ggatcctggc cgaacagttc gacgctgccg ctgtcggcgc
      241 gcagcaggcc aagcaacagg ccgatcgcgg tggtcttgcc ggcaccgttc gcgcccagca
      301 gcgacgtcac ctgcccggcg cgcaacgaca ggtccagccc atcgagcgcg cgcaccttgc
      361 cgtaagactt ggtggcctgc tgcaggcggg ccagcacatc gcggttgcca tgcatcgcac
      421 atctccccgt ggtcgatgcc gcgcatcctc cgcgcaagcc atcgccgccg gcagtcgcgg
      481 gtttcaggca aacgacatga caagcgtcag gtgacgccgc gccggcgcag gcctagcctg
      541 cgcagcgtcg gccgatggca cgcgccccgc catgccgtca cactgatcgt ccctccgcat
      601 ccctccagac cgcgcatgaa ctcgtccgca gacctgctga agtcgctcaa gatcgaccgc
      661 agcgcaccac caccgcccac gtcacgcaag ggcctgtgga tcggcctggg cgtggccgcg
      721 gcggtcgtgc tgggcgcggg tggctggttc ttgctcggcc gcgacagcgc agtgcaggtg
      781 gagacgacca gtgcggtatc gctcgccagc ggcgccggca acgcctccgt actggatgcc
      841 agcggctacg tggtggcgcg acggatggcc accgtgtcgt cgaagatcac cggccgcgtg
      901 cgcgaggtgt tgatcgaaga gggccagcgg gtcgaggaag gccaggtgat ggccacgctg
      961 gatccgatcg atgccgatgc ccagcgcgat ctctccgcgg cgcagcttgc cgccgcgcag
     1021 agccagatcg tcggcaccca ggcgcagttg aaggaggccg aggcgaatgc cgcgcggctc
     1081 ggcgcgctgg tcggccagaa gctggtctcg cgcgcgcagt tcgaacaggc cgtggcccag
     1141 cgcgacgcct tgcgcgcgca ggtcgccacc gcgcagcgca atgcgcaagt ggcgcgcagc
     1201 agcctggcca tcgccagcaa tggcgtggac aacaccgtgg tgcgggcgcc gtttgccggc
     1261 gtggtgatcg ccaaggcggc gcagccgggc gaaatcgtgt cgccgctgtc ggcaggcggt
     1321 ggcttcaccc gcaccggcat cggcaccatc gtcgacatgg attcgctgga ggtcgaagtg
     1381 gatgtcggcg aggcctacat cggccgggtc aaaccgaaga tgccggtcga agccacgctc
     1441 aattcctatc ccgactggaa gatcccggcc gaagtgatcg cgatcatccc caccgccgat
     1501 cgcggcaagg cgacggtcaa ggtgcgggtg gcgttgaagg cacgcgacgg ccgcatcgtc
     1561 ccggacatgg gcgtgaccgt cagcttcctg gaaaaagccg ccgacgcgac cgccgaaccg
     1621 aagcgcggcg tgcgcgtccc cgcccgcgcg atcgtccagc gcgatggcgg cgacgccgcg
     1681 ttcgtggtcg gcgccgagga catcgtcgaa ttgcgcaagg tacaggtcgg tggcgcactc
     1741 ggcgaagacc gcgaggtcag ctccggcctg gcacctggcg aaaccgtggt gatcgcacca
     1801 cccgccacgt tggccgacgg cgaccgcatc gccaccaaga ccgaatgaac ccgcgggcgg
     1861 caggaacacc gccgcccacc cgaaccaccg acacgacatc catcgacgag gcagccatgg
     1921 catccctggt atcgacccgc aacctgacga agacctacca gcgcggcccc gagaaggtcg
     1981 aggtgctgca cggcatcgac ctggacatcg cggaaggcga tttcgtcgcg ctgatgggac
     2041 cgtccggctc cggcaagacc accttgctca acctgatcgg cggcctggac tcgcccagcg
     2101 gtggcaccat cgaggtggcg ggccagcgca tcgaccagct cggcggcggc aaactggccc
     2161 agtggcgcag ccagcacgtc ggttacgtat tccagttcta caacctgatg ccggcattga
     2221 ccgcgcagaa gaacgtcgag ttgccgctgc tgctgacccg cctgtccggc gcgcagcgca
     2281 aacgcaacgc gcaggtcgcg ctgcaactgg tgggcttgtc cgaccgcgtc agccacaagc
     2341 cgaccgagtt gtccggcggc cagcagcaac gcgtggcgat cgcccgcgcg atcgtgtccg
     2401 acccgaccct cctcatctgc gacgaaccca ccggcgacct ggaccgccag tccgcggaag
     2461 acatcctggg cctgctgcaa acgctcaacc gcgagcacgg caagaccatt gtcatggtca
     2521 cccacgatcc caaggccgcc gggtacgcga gccataccct gcacctggac aagggcacgc
     2581 tggtcggcga ccacgccgat gcggcctgac gccgacctcg ggagcatgcg atgaaatatt
     2641 tccatctcat ctgggcggcg ctgttccggc gcaagacccg cacgttcctc accctggcgt
     2701 cgatcctggc agcgttcctg ctgttcggca tgctggatgg cattcgcacc acgttcgacc
     2761 agctcggccg caacgcggac ggcgcgcagc gcctgcagac gtcctccagg ttgtcgttca
     2821 tggacagctt gccgttgtcg ctgcaatcgc ggctggaacg ggtggacaac atcgaggccg
     2881 tcacgcacgc caactggttc ggcggcgtgt accagaaccc gaagaaccaa ctgttcacct
     2941 tcgcggtggc ggacaactac ctcgacctct acccggagat gctggttgca cccgccgagc
     3001 gcgaagcatg gatgcgcacg cgcaccggca tcctcgtcgg cgaggcaatg atgaagcgct
     3061 tcggttggaa ggtcgggcag aagatcccgt tgcagtcgaa cattttcccg aacagcgacg
     3121 gcaccctgaa ctgggcattc gacatcgttg gcacgatcaa ggccaaggac ggcaagggcg
     3181 gcggtttcac cgacgccgtg atcctcatgc actacaagta tttcgaggaa tccacgtcct
     3241 acgtggacgg cgacgtgggc tggtacatca gccgcgtggc ggacgtgcgc cggagcgacg
     3301 cggcggcgaa agcaatcgat gcgttgtcgg tgaattcgca gcacgaaacc aagaccatga
     3361 acgagcaggc ggcgatggcc gcgcagctca agcaggtggc cgacatcggc atgatcgtgg
     3421 gttcgatcat gggcgcggtg ttcttcaccc tggtgctgct gaccggcaac accatggcgc
     3481 aggcggtgcg cgagcgcact tcggaactgg ccgtgctcaa gaccatcggc ttcaccagcg
     3541 ccagcgtgct cggcatggtc ctggccgaat cggtgctgct ggtgctgatc ggcggcgtgc
     3601 tcgggttggg cctggccgcc gtgctggcgc cgatcgtcgg cgcggccagc ggtggcgcga
     3661 tcaacctgcc gccgatcggt tggcggagct ggtcgctggg actggcgctg atggtgggca
     3721 tcggcctgct ggtaggcgcg ctgccggcga tgcgggcgat gcgcctgaac atcgtcgacg
     3781 cgctcgcggg ccgctgagga ggaaccggac atgaagaaga tcaaatcgtt cctgcgcggc
     3841 cttgccctgc tcctggtgct cgcggtcatc cttgctgcgt ggatcgtatt gccgtggcct
     3901 gcactgctgg gcgcagccgt gctgttcggg ctgtggatgg cgttcacccg cagcggccgt
     3961 caggccgcct cggtgaccca ggtcggcatc agcaccctga accagcgcat tggcgcatcg
     4021 tcggtggtgg tgatcggcat cgccggcgtg gtcggcgtgc tggtggcgct gctcgcgatg
     4081 ggcgaaggct atcggcagac ggtcagcaac agcggcagcg aggacaccgc catcgtgctg
     4141 cgcggcggtt catccgccga gttggggtct gtgctgtcgc gcgaccagat cagcgccatt
     4201 gaacgtgcac cgcagatcgc gcgcgacgcg caaggtcgcc cgctggcatc agcggagctg
     4261 atggtggccg ccaccgtccc gctcaaaggc agcaaggacg atggcaatgc cagcctgcgt
     4321 ggcgtcagcg agatggccta tgcggtgcgg cccaagctga agctggtggc tgggcgccgc
     4381 ttcgaacctg gccgccgcga gctggtggtg ggcacgggtg cgcagcgcca attcgatggg
     4441 ttggaggtgg gcaagcaggt gcgcttgggc aaccagccgt ggacagtggt cggcgtgttc
     4501 gattcgcagg atgcgaccgc ttcggaaatg tgggccgacg ccgaagtcgt cgctgccacc
     4561 taccgccgcg gcagcagccg cgcagcgctg tacgcgaaac tgaccagcgc gaaagacttc
     4621 aatgcgttca aggcgaccct ggcggccgat ccgcgcctgc aggtcgaagc cgacacgacc
     4681 ctggcctact tcggcaagca gtccgaggga ctgaccaagg tgctacgcat catcggcatc
     4741 gtggtcggaa gcatcatggc catcggcgca gtcttcggtg cactcaacac catgttcgcc
     4801 accgtcgcct cgcgcgcgcg cgaaatcgcc acgctgcgcg cgatcggctt ccgcggcatc
     4861 ccggtcgtgg tcgcggtgat gctggagacc atgctgttgg cgttgctggg cggcgttctc
     4921 ggcgggctgg tggcgtggct gctgttcaac ggccataccg cctcgaccct ggccggcggc
     4981 atgtcgcaac tcagcttcca gttcaaggtc gatcccacgt tgctctggga agggctgaag
     5041 tgggcgctcg cgatcggttt catcggcggc ctgttcccgg ccctgcgcgc ggccagcctg
     5101 ccggtcaccg acgccctgcg cagcgcctga tcgtcgctgc gcgacgggcg gccggctgaa
     5161 tgcccgccgc ctgtcatcgc actgtcatgc caatgcggta gatgcgcggg ccgcgggccc
     5221 ctaccctgcg cgacccccta ccgtctggat cgcgcccatg cgtcgcgcgc tgctcatcct
     5281 gtgcgcctgc ctgttgctgg ccggcttcgc ctggcccgac gccgccgaca tgctcggcaa
     5341 gcgcctggtc gcgcccggcg gtgtctcgcc gatcctgccg accacgcgca tcgaagccag
     5401 catcgcccgg ctgccgcacc ggcaaggctg ggtgtcgact tccgctggcg tgaagttgtt
     5461 ctggcgcgcg ttcgatcccg gtgcctaccg gctcgactac gcctaccgcc cggatggcac
     5521 ccaggccggg cgcatgcagt tcgacctcga tgcagccgcg cccgtacgct atccgggcgc
     5581 gccgcgcggc acggtggtgt tgctgcacgg ctggatgatg gacggcggct ctttgctgcc
     5641 gtggtcgctg caactcgcgc aggccggcta ccgcacgatt accctggacc tgcgcaacca
     5701 tggccgctcc ggcgcggcac cctccggtta cggccagcgc gaggcgcgcg acgtggccga
     5761 tgcgatcacg gcgctgcgcg agcgcggcga ggtggtcggc ccgctgcacc tgatgggcgt
     5821 gtcctacggc gcggcgaccg cgatcttcag cgcgcgcgaa ctgggcgcag aggtcaacaa
     5881 cgtcatcgcc atcgaatcgt tcgacaacgc cggtggcgcg atccgcgaca tggtcccgca
     5941 catgctggcc aagccgccgg aaagcatgtc ccagcgcatc tcccatcgcc tgctgcgcat
     6001 gcaactggac gacggcgcgc ttgagcgcgc gatcgcctcc gccagcgaca ccctgcagtt
     6061 gcggctggac cgcgtcgacg tgggtgcggc gctcgcgcag gtgccgtcct gcgtgttgct
     6121 gctgcacggc cgcgaggacc agcacgtgcc ggtcgcgcat ggccgcgcac tggccgccgc
     6181 cgcgccgcgc gcgcactaca tcgaggtggc cggcgaggac cacatcagcc tgccgatgcg
     6241 gctggacctg
//
LOCUS       DAOOCX010000129         6231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11811_length_6231_cov_7.084845, whole genome shotgun sequence.
ACCESSION   DAOOCX010000129 DAOOCX010000000
VERSION     DAOOCX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11811_length_6231_cov_7.084845"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PK399_10305"
     CDS             complement(<1..829)
                     /locus_tag="PK399_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW13550.1"
                     /translation="MTHPNRVRLSKLSLGLMVALAAAPVFAQSTSAGVGGQVVSADGQ
                     PVAGAEVTITHIESGTVSRATTDAAGRYSARGLRVGGPYTITINKAGAGSDTEENVYL
                     ALNQVGTVNGQLSNDVNTLGTVVAVGTAGSYVFGSENKGVGTNIGGRALETAVSGNRS
                     IDDIARLDPRVNITEQSEGSISVSGQNNRYNNISVDGLSVGDPFGLQAGGLAFIGSPI
                     SADSISAYDIKISDFDTTSDSVGANINAVTKSGTNEFHGSVYYAFSKASSMVGEFNGN
                     "
     gene            complement(1053..1646)
                     /locus_tag="PK399_10310"
     CDS             complement(1053..1646)
                     /locus_tag="PK399_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPW13551.1"
                     /translation="MTIASAALLLFLILDPLGNIPVFLGLLKDLPPARQRRVLARELL
                     IALGVLMLFLWGGQYALEVMHLRQESVSIAGGIVLFLIGLRMIFPPADGIMGELPDGE
                     PFIVPIAIPMIAGPSGMAAVMLLGSQEPGRMGDWMLALTIAWAATAVILFSATYLKKW
                     LGTRVLTAIERLMGMVIVAISVQMLMDGISTYRGAGI"
     gene            complement(1657..2379)
                     /locus_tag="PK399_10315"
     CDS             complement(1657..2379)
                     /locus_tag="PK399_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW13552.1"
                     /translation="MSSTTSLEGRVVLVAGAAGGLGSAASVACARAGATVVLLGRKVA
                     SLNRVYDAASAVGPEPLLYPLDLVGATPDDYTELASRVEAELGRLDGVLHCAAEFKGL
                     TPLLHTDPADFARAIHVDLTARWWLTQACLPLLAKSDAGAVVFALDDPLRTDGAFWGG
                     YGLAQSAQASLVSMLQAELGEHGPRISGLRPGPMRTALRGKAYQPNADAEARAPTDYA
                     AACVDLLSSIGSAARGRIVDAA"
     gene            complement(2376..2693)
                     /gene="grxD"
                     /locus_tag="PK399_10320"
     CDS             complement(2376..2693)
                     /gene="grxD"
                     /locus_tag="PK399_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HPW13553.1"
                     /translation="MSVEDRIRAEIEGHPIVLFMKGTPQFPMCGFSSRSVQALKAAGA
                     QLHTVNVLEEPEIRANLPRYSDWPTFPQLFIKGELIGGCDITVELAESGELARIVADA
                     HKA"
     gene            2841..3419
                     /gene="sodB"
                     /locus_tag="PK399_10325"
     CDS             2841..3419
                     /gene="sodB"
                     /locus_tag="PK399_10325"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HPW13554.1"
                     /translation="MAIELPALPYDRTALEPHISGETIDFHYGKHHKTYVDNLNKMIE
                     GTEFADMALEEIIRKSQGGMFNNAAQIWNHTFYWNCLKPAGGGEPTGKLADAINAAFG
                     DFAKFKEQFTDTSIKTFGSGWGWLVQRKDGSLALASTSNAATPLTGEDTPLLTCDVWE
                     HAYYIDYRNARPKYLDAFWNLVNWDFVASNMK"
     gene            3589..4017
                     /locus_tag="PK399_10330"
     CDS             3589..4017
                     /locus_tag="PK399_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13555.1"
                     /translation="MNMRHLIASSLCLIALGAATTADAAQRRDRDNNPPGPAGGRGTN
                     WENPPGWKGGPGASPDRRWYRHGGKRYEFKVARNGYYFSQHYGYWHPRFGLWNQARRC
                     WLDLDNNPPGPRGGRGTNWENPPGWKGGPGASPDRFGRCR"
     gene            complement(4087..5256)
                     /locus_tag="PK399_10335"
     CDS             complement(4087..5256)
                     /locus_tag="PK399_10335"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HPW13556.1"
                     /translation="MHASKAAASMKTVGILGGGQLARMMALSAAPLGLRFLVLDTAAD
                     ACAAQFAPMVVGDYTDEAALAEFASKIDVATFDFENVPAESAQWLAARVPVFPSPRAL
                     AVAQDRLAEKTLFRELGIPVPEFADIDSRAALDAAVVRLGTPCILKTRRLGYDGKGQF
                     RIKSPADVDAAWEALGAQAGTVGLILEGFVAFQRELSVVAVRGQRGEFRAWPLTENWH
                     VDGVLSASLAPARVDDAMSAVAMSHARKLADALDYVGVFALELFCRDGELLANELAPR
                     VHNSGHWTIEGSETSQFQNHLRAVLGLPLGDTRMLGVACMLNWIGEMPDAAPVLREAS
                     GHWHDYGKEPRAGRKVGHATLRAESADELAGALQRVGDALGRQAQVAPVIERLRG"
     gene            complement(5243..5761)
                     /gene="purE"
                     /locus_tag="PK399_10340"
     CDS             complement(5243..5761)
                     /gene="purE"
                     /locus_tag="PK399_10340"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007971425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPW13557.1"
                     /translation="MTGSADASDQGRPLVGIVMGSRSDWETMQHAALRLEALGVAHEV
                     RVVSAHRTPDVLFEYAASASSRGLRAIIAGAGGAAHLPGMLAAKTVVPVLGVPVQSKA
                     LNGMDSLLSIVQMPAGIPVATFAIGNAGAANAALFAAAMLASEHAAIGAALDAFRAQQ
                     TDDVARNDDPRK"
     gene            5845..6114
                     /locus_tag="PK399_10345"
     CDS             5845..6114
                     /locus_tag="PK399_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPW13558.1"
                     /translation="MDRKLLDILACPATRMPLAMLEAPGLDALNRAIAAGGLRLNDDS
                     LQTALLREALVTRDRKTVYRIDDGIPVLLTEEGIATAQVADFPKA"
     gene            6111..>6231
                     /locus_tag="PK399_10350"
     CDS             6111..>6231
                     /locus_tag="PK399_10350"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448462.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase"
                     /protein_id="HPW13559.1"
                     /translation="MSAGNRFAPPPAEVIAANVDAALAEDLGPGDATASLLDDT"
BASE COUNT         1073 a         2174 c         2064 g          920 t
ORIGIN      
        1 cgttgccgtt gaactcgccg accatgctgc tggctttgct gaacgcgtag tacaccgaac
       61 cgtggaactc gttggtaccc gacttggtga ccgcgttgat gttggcgcca acggagtccg
      121 aggtggtgtc gaagtcgctg atcttgatgt cgtaggcgga aatggaatcc gcggagatgg
      181 gcgaaccgat gaaggccagg ccaccggcct gcaggccgaa cgggtcgccg accgacaggc
      241 catcgacgct gatgttgttg tagcggttgt tctggccgga aaccgagatc gagccttcgc
      301 tctgctcggt gatgttgacg cgcggatcga ggcgggcgat gtcgtcgatc gaccggttgc
      361 cgctgaccgc ggtctccagc gcgcggccgc cgatgttggt gccaacgccc ttgttctccg
      421 aaccgaagac gtaggatccg gcggtgccga cggcgaccac cgtgccgagc gtgttgacgt
      481 cgttgctcag ctggccattg acggtaccga cctggttcag ggccaggtag acgttttctt
      541 cggtatcgct accggcgccc gccttgttga tggtgatggt gtacggacca cccacgcgca
      601 ggccgcgcgc ggagtagcgg ccggccgcat cggtcgtggc gcggctgaca gtgcccgact
      661 cgatgtgggt gatggtgact tcggcgcccg cgaccggctg gccgtcggca ctgaccacct
      721 ggccgccaac gccggcggac gtgctctgcg cgaaaacagg agccgcggcg agcgcgacca
      781 tcaggcccag cgacagcttc gacagccgga cgcggttcgg gtgagtcata tcggtaacct
      841 cagtgagcga aaaaagaaaa gaacgacgga cgaaggacga aggacggcac atgccgacgc
      901 gtccgtcctg cgacggttgt cggcgaattc ggaacccctg caccatgcga agccggatgg
      961 gttcaccaaa gtttaacacg gtaggcggaa ttcatgaccg ccagatgaca gccgtgggac
     1021 gcccggcaaa acccgtaagt ggccgcccct gttcagattc cggcgccccg ataggtcgag
     1081 atcccgtcca tcagcatctg caccgaaatg gccacgatga ccatgcccat cagtcgctcg
     1141 atcgcggtca acacccgcgt ccccagccac ttcttcaggt aggtggcgga gaacaggatc
     1201 accgcggtcg ccgcccatgc gatggtcagc gccagcatcc agtcgcccat ccgtcccggc
     1261 tcctggctgc ccagcagcat caccgcggcc atcccggaag gcccggcgat catcgggatg
     1321 gcgatcggca cgatgaaggg ctcgccgtcg ggcaactccc ccatgatccc gtccgccggc
     1381 gggaagatca tccgcaggcc aatcaggaac aggacgatgc cccctgcgat ggacacagac
     1441 tcctggcgca ggtgcatcac ttccagggcg tactggccgc cccacaggaa cagcatcaac
     1501 acgcccagcg cgatcagcag ctcgcgcgcc aacacccggc gctggcgtgc cggcggcagg
     1561 tccttgagca agcccaggaa caccgggatg ttgcccagcg gatccaggat caggaacagc
     1621 agcagcgcgg ccgaggcgat ggtcatgcgt gcgtggtcag gcggcgtcga cgatccggcc
     1681 gcgcgcagcc gaaccgatgg acgacagcag gtcgacgcag gccgcggcgt agtcggtcgg
     1741 ggcgcgagcc tccgcatcgg cattcggctg ataggccttg ccgcgcagcg cggtgcgcat
     1801 cgggccgggt cgcaagccgc tgatgcgcgg cccgtgttcg cccagttcgg cctgcagcat
     1861 ggacaccagg ctggcctgtg ccgactgggc caggccatag ccgccccaga atgcgccgtc
     1921 ggtgcgcagc ggatcatcca gcgcgaacac cactgcaccg gcatcggatt tcgcgagcag
     1981 cggcaggcag gcctgggtca gccaccagcg cgcagtcagg tcgacatgga tggcgcgggc
     2041 gaaatcagcg gggtcggtat gcagcagcgg cgtcaggccc ttgaactcgg cggcgcaatg
     2101 caggacgcca tcgagacggc ccagttccgc ttcgacgcgc gacgcgagtt cggtgtagtc
     2161 gtccggcgtg gcgccgacca ggtcaagcgg gtaaagcagc ggctcggggc cgacggcgct
     2221 cgccgcgtcg tacacgcgat tgagcgacgc gaccttgcgg cccagcagga ccacggtcgc
     2281 acccgcgcgt gcgcaggcca cggacgcggc cgaacccagg ccgccggcgg caccggcgac
     2341 caggacgacg cgaccttcga gcgacgtggt gctgctcacg ccttgtgcgc gtcggcgacg
     2401 atgcgggcca gctcgccgga ctcggcaagc tcgacggtga tgtcgcaacc gccgatcagt
     2461 tcgcccttga tgaagagctg cgggaacgtg ggccaatcgg agtagcgcgg caggttggcg
     2521 cggatttccg gttcctccag cacgttcacg gtgtgcagtt gcgcgcctgc ggccttgagc
     2581 gcttgcaccg agcgactgga aaagccgcac atcgggaact gcggcgtgcc cttcatgaag
     2641 aggacgatgg ggtggccttc gatctcggcc cggatgcggt cttccacgga catggttcaa
     2701 ctcctgcagc gttcgcccgc cgcgggcttg acggcctggc aacgggcggt ggggatagaa
     2761 tgccggttcg gcgacacgcg cattgtaagt cttcgcgcgg ccccggccgc acccccgcca
     2821 cttccgacag gagcccgccc atggccatcg aactgccagc cctgccctac gaccgcaccg
     2881 ccctggaacc gcatatttcc ggcgaaacca tcgacttcca ctacggcaag caccacaaga
     2941 cctacgtgga caacctcaac aagatgatcg agggcaccga gttcgccgac atggcgctgg
     3001 aggagatcat ccgcaagtcg cagggcggga tgttcaacaa cgccgcgcag atctggaacc
     3061 acaccttcta ctggaactgc ctgaagcccg ccggcggcgg cgagccgacc ggcaagctgg
     3121 ccgacgcgat caatgccgcg ttcggcgact tcgccaagtt caaggaacag ttcaccgata
     3181 cctcgatcaa gaccttcggc tccggctggg gctggctggt gcaacgcaag gacggctcgc
     3241 tggcgctggc cagcacctcg aacgcggcaa ccccgctgac cggcgaagac accccgctgc
     3301 tgacctgcga cgtgtgggaa cacgcctact acatcgacta ccgcaacgcc cgcccgaagt
     3361 acctggacgc attctggaac ttggtgaact gggatttcgt cgccagcaac atgaagtaag
     3421 ccatccagca atggcatgca cgcgacggcc ggggcaaccc ggccgtttgc gtttcccggc
     3481 gacgcggatt tcctgtgcgc gccctgaaca tggcggccga cgtcgtcaac gatgcgcgtg
     3541 cccgcgcctt tccggaaacg tggttcccac ccggaaagga gtcaagccat gaacatgcgc
     3601 cacctgatcg cctccagcct gtgcctcatc gccctcggtg ccgcgacgac ggccgatgcc
     3661 gcgcaacgcc gcgaccgcga caacaacccg cccggcccgg ctggcggccg cgggaccaac
     3721 tgggaaaacc cgccgggctg gaaggggggg ccgggtgcct cgccggatcg tcgctggtat
     3781 cgccatggcg ggaagcgtta cgaattcaag gtggccagga acggctacta cttcagccag
     3841 cactacggct actggcatcc ccgcttcggg ctctggaacc aggcgcgccg ttgctggctg
     3901 gacctggaca acaacccccc ggggccgcgc ggtggccgcg gcaccaactg ggaaaatccg
     3961 ccaggctgga agggggggcc gggtgcctcg cccgatcgct ttggccgctg ccgctgaggc
     4021 gcggcatcgc aacacgacaa cggccggatc tccggccgtt gtttttggcg cattccccgt
     4081 atgcgcctat ccgcgcaagc gctcgatcac cggcgcgact tgcgcctgcc gcccgagggc
     4141 atcgccgacg cgttgcagcg cgcccgccaa ctcgtccgcg gattcggcac gcagcgtggc
     4201 atgcccgacc ttgcggccgg cgcgcggctc cttgccatag tcgtgccaat gtccgctggc
     4261 ctcgcgcagg accggcgcgg catccggcat ctcgccgatc cagttgagca tgcaggccac
     4321 gccgagcatc cgcgtatcgc ccagcggcaa gccgagcacg gcacgcaggt ggttctggaa
     4381 ttgcgacgtc tcgctgcctt cgatggtcca gtggccggag ttgtgcacgc gcggcgccag
     4441 ttcgttggcc agcagttcgc cgtcgcggca gaacagctcc agcgcgaaca cccccacgta
     4501 gtccagcgcg tcggcgagct tgcgtgcatg cgacatcgcg acggcactca tcgcatcgtc
     4561 cacccgcgcc ggcgccaggc tggccgacag cacgccatcg acatgccagt tctcggtgag
     4621 tggccaggcg cggaattcac cgcgctgccc acgcaccgcg accacgctga gttcgcgctg
     4681 gaacgcgacg aagccctcca ggatcaggcc gaccgtgcca gcctgcgcgc ccagggcctc
     4741 ccacgcggca tcgacatccg ccggcgactt gatccggaac tggcccttgc cgtcgtagcc
     4801 gaggcgacgc gtcttcagga tgcagggcgt gcccagcctc accacggccg catcgagcgc
     4861 ggcgcgcgaa tcgatgtcgg cgaactccgg caccgggatg ccgagctcgc ggaacagggt
     4921 cttctcggcc agccggtcct gcgcgacggc gagcgcacgc gggctcggga acaccggcac
     4981 gcgcgccgcc agccactgcg cggattccgc cggcacgttc tcgaagtcga acgtcgcgac
     5041 atcgatcttc gaggcgaatt cggccagcgc ggcctcgtcg gtgtagtcgc cgaccaccat
     5101 cggcgcgaac tgcgccgcgc aggcgtcggc ggcggtatcg aggaccagga agcgcaggcc
     5161 cagcggcgcg gcggacagcg ccatcatccg cgccaattgc ccgccgccga ggatgccgac
     5221 cgtcttcatc gatgcggccg ccttacttgc gtggatcgtc gttgcgggcg acgtcgtcgg
     5281 tctgttgcgc acggaaggcg tcgagcgcag cgccgatcgc cgcgtgctcc gatgcgagca
     5341 tcgccgcggc gaacagcgcc gcattcgcgg caccggcatt gccgatggcg aaggtcgcca
     5401 ccggtatccc ggccggcatc tgcacgatcg acagcagcga atccatcccg ttcaacgcct
     5461 tgctctgcac cggcacgccc agcaccggca ccacggtctt ggcggccagc atgcccggca
     5521 ggtgcgccgc gccgccggcg ccggcgatga tcgcgcgcag gccgcgcgat gatgcgctgg
     5581 cggcgtattc gaacaagacg tccggcgtgc gatgcgcgga gaccacgcgg acttcgtgcg
     5641 ccacgcccag cgcttccagg cgcagcgccg catgctgcat cgtctcccag tcggagcgcg
     5701 agcccatcac gatgccgacg agtggcctgc cctggtcaga tgcgtccgcg ctgcctgtca
     5761 tcacgatgcc tgcgccgtgg cgcccgatcc aaaccggtat tctagcgatc ccacccgtca
     5821 tcggcgcgcg tcgccgggaa ccgcatggac cgcaagctgc tcgacatcct cgcctgcccc
     5881 gccacccgca tgccgttggc gatgctggaa gcccccgggc tggacgcact gaaccgcgcg
     5941 atcgccgcag gcgggttgcg cctgaacgac gacagcctgc agaccgcact gctgcgcgag
     6001 gccttggtca cccgcgaccg caagaccgtg taccgaatcg acgacggcat cccggtgctg
     6061 ctcaccgagg aaggcatcgc caccgcgcag gtcgctgact tccccaaggc atgagcgctg
     6121 gcaaccggtt cgcgccgccc ccggccgagg tcatcgcggc gaatgtcgat gcggcgctgg
     6181 ccgaggacct cggccccggc gatgccacgg cgtcgctgct cgatgacacg c
//
LOCUS       DAOOCX010000130         6225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_11826_length_6225_cov_7.804862, whole genome shotgun sequence.
ACCESSION   DAOOCX010000130 DAOOCX010000000
VERSION     DAOOCX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11826_length_6225_cov_7.804862"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1144)
                     /locus_tag="PK399_10355"
     CDS             complement(98..1144)
                     /locus_tag="PK399_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPW13560.1"
                     /translation="MRKTMLATMVAMAMMVGPVHAQVAEAPADTPSTPQQAAVGADGI
                     RTEATVIVDGTQPGPGLWLVRKGAHDLWILGTLSPLPAKMQWQSKQVQGVIGNAQEVI
                     YPPRVSLDVKAGFFQKLTLLPAMLGARKSPDGKRLEEIVSPASYARWKTLKARYIGGD
                     GGVEKWRPLFAAQELYKEAMKKSGLDNGKSVWPVINDAVESHHPNVTVVKEEIVVTDP
                     KPLLKEWSKTTLDDIACFDNTMTRIETDLDAMRARANAWATGDMAALQSLPPPYQWES
                     CSDAITEAGIGKRLGFGDANQKVRAKWLAAAEAALEKNSVTFAVLDMKEMTTADGLVA
                     KLKAKGYMVLAPDE"
     gene            complement(1249..1632)
                     /locus_tag="PK399_10360"
     CDS             complement(1249..1632)
                     /locus_tag="PK399_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HPW13561.1"
                     /translation="MSRIADPRITLLLRARRVLGAVGSVLAGVAVALAAYAAHGVDGQ
                     AQARLAQAAAFAFAHGLALAALAPLVQRRVGLVALLAMLSGVLLFSGSLVGAAAWSLP
                     TTLAPFGGMLMIVGWLLHGADRLRG"
     gene            complement(1727..2794)
                     /locus_tag="PK399_10365"
     CDS             complement(1727..2794)
                     /locus_tag="PK399_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HPW13562.1"
                     /translation="MTRLLEILISLAIVAGLFLVVALVLPSSRHLVEKVETNRKLTIA
                     FDSLNSLRRFKDWNPLILRDPRVQLTLSGPDSGVGARLDYVSKEEGLGKGSWEITESV
                     PREKVSYKIVNEERGGNKRTSFSFKPTGRNNRNVEITQSYDVDYGWNLLGRYAGMYVS
                     RHVGDDMKLGLSRIVGMLASVPNVDYAVSGSKMTGLKVVDRPVEDILFVNAGNVERGN
                     SQIQASISANAEWLKRVIEANGLEAVGPLRIISTELGRETYNFDVAQAVRKKDGSAVG
                     KPALQGPVQHVQTPAAKVATAAYTGYMAELDNVRNALRAWALTSGYEVVDRPYESYKS
                     GVAGAFTENGEFDVYWVLKSK"
     assembly_gap    3080..3179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3317..3988)
                     /locus_tag="PK399_10370"
     CDS             complement(3317..3988)
                     /locus_tag="PK399_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW13563.1"
                     /translation="MTHPAPSNAAPIRVFLVDDQTLVRQGIRSLLALAEGIEVVAEAS
                     DGRQAVEQIPQVAPDVVLMDMRMPVMSGLESLQAMSRAGILPPTIILTTFDDDALVLA
                     GIKAGAKGYLLKDVSLEQLVNAIQMVAAGGSLVQPAVTQRLLSGLEHMRNEFVSLDRP
                     DPLTDRETEILRLMASGFSNKEIANSLGVAEGTIKNHVSNILSKLGVRDRTRAVLKAF
                     ELQLV"
     gene            complement(3985..5187)
                     /locus_tag="PK399_10375"
     CDS             complement(3985..5187)
                     /locus_tag="PK399_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPW13564.1"
                     /translation="MLARISHTRLLRYAGLFTWAMVGLPLVYSWVGPLLGTAGDEELA
                     LRPMPWQGWLAYLAFGLSYAWLTRGLGSRSRSVGDYILLVVLTLAALAVSYFNGSGLG
                     SVLLMVAACVLPWLLPLPLGAAWLLASQLAVAPVFIRWLGFPPLEAVMQSVLYAGFSG
                     FVFITSLVALQQMQAREDQRRLNAELRATRALLADSARVNERTRISRELHDLLGHHLT
                     ALSLNLEVAGHLSEGRVKEHVQQAHTLARLLLTDVREAVSQLRESGAIDLGAALRPLA
                     ENVPKLAIEMDIEQPLTVDDPERAHILLRCAQEAITNVVRHAGASRLWLSARREGPDV
                     ILQIRDDGRGSDQVQPGNGLRGLHERLRQQDGRLEIATRGGHGFQLTMTLPVAPNAAT
                     GLPQGVAA"
     gene            5321..5920
                     /locus_tag="PK399_10380"
     CDS             5321..5920
                     /locus_tag="PK399_10380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW13565.1"
                     /translation="MSIAIRDVREHELDSVLALNNAAGPAILPLDAARLRHLFDCAEH
                     FRVAERDGALAGFLIGFGAHARHDSSNFAWFGERYPDFFYIDRVAVASRRRGGGVGRA
                     LYADAQSYAELRYPQLACEVFLQQGNDHALLFHGSFGFREVGQHVMPGHDIRAAMLMK
                     GLCSYAWVRDTYGDALPGEPWLPQPRALAQASRPTGTCA"
     gene            5911..>6225
                     /gene="minC"
                     /locus_tag="PK399_10385"
     CDS             5911..>6225
                     /gene="minC"
                     /locus_tag="PK399_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399452.1"
                     /note="blocks the formation of polar Z-ring septums;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HPW13566.1"
                     /translation="MRVSAVVDYAQAGELKFGQVGIANLRVRTLDVAQLAAEMRDRVQ
                     RAPNLFARAAVVLDFGGLSQVPAVDEARALLEGLRDAGVLPVALAYGTKEIDALSQQL
                     GLP"
BASE COUNT          978 a         2163 c         1999 g          985 t
ORIGIN      
        1 cggcatccgc aatgcggtgc tcaagcgctg gccacacgcg acggtgccgg cgcagccacg
       61 cgccgcggcg tcctgatcgc cgcggctcgc gcctgtctca ttcgtccggc gccagcacca
      121 tgtagccctt ggccttcagc ttggccacca agccgtcggc cgtggtcatt tccttcatgt
      181 ccagcaccgc gaaggtgacg ctgttctttt ccagcgcggc ctcggcggcg gcaagccact
      241 tggcgcggac tttctggttg gcatcaccga agcccaatcg cttgccgatg ccggcttcgg
      301 tgatcgcgtc gctgcaagac tcccactggt acggcggcgg cagcgattgc aacgcggcca
      361 tgtcgccggt cgcccaggca ttggcgcgcg cgcgcatggc gtcgaggtcg gtctcgatgc
      421 gggtcatggt gttgtcgaag caggcgatgt cgtccaacgt cgtcttcgac cactccttga
      481 gcagcggctt cggatccgtg accacgattt cttccttcac cacggtcacg ttgggatgat
      541 gggattcgac cgcgtcgttg atcaccggcc acaccgactt cccgttgtcc aagccggatt
      601 tcttcatcgc ctccttgtac aactcctgcg cggcgaacaa agggcgccac ttctcgacgc
      661 caccgtcgcc accgatgtac cgcgccttca atgtcttcca gcgcgcgtac gacgcgggtg
      721 acacgatctc ctcgaggcgc ttgccgtccg ggctcttgcg cgcccccagc atggcgggca
      781 gcagggtcag cttctggaag aacccggcct tgacgtccag gctgacgcgc ggcggataaa
      841 tcacctcctg cgcattaccg atcacgccct gcacctgctt cgattgccac tgcatcttgg
      901 caggcaacgg actcagggtg cccaggatcc acaggtcatg cgcgcccttg cgcaccagcc
      961 agaggcccgg ccccggctgc gtgccatcga cgatcaccgt cgcctcggta cggatgccgt
     1021 cggcaccgac ggcggcctgc tgcggcgtcg agggcgtgtc cgccggtgcc tcggcaactt
     1081 gcgcatgcac cggtcccacc atcatcgcca tcgcgaccat cgtcgccagc atcgtcttcc
     1141 gcatgtcatt cccgtcatgg cgcgttgcgc gcggtggcgc agtgtgaccg gggtcgcatc
     1201 gcgaagttca accggcacct gaatcggcgc acgtcgaagc gtcctgcctc agccgcgcaa
     1261 gcgatccgcg ccatgcagca accagccgac gatcatcagc atgccgccga acggcgccag
     1321 cgtggtcggc aggctccacg ccgccgcgcc caccaggctg ccggaaaaca gcagcacgcc
     1381 ggacaacatc gccagcaaag cgaccaagcc aacgcgtcgc tgcaccaacg gagccagtgc
     1441 agccagcgcc agtccgtgcg cgaaggcgaa ggctgcagct tgcgcgaggc gcgcctgtgc
     1501 ctggccatcg acgccatgcg cggcataggc tgccagggcc accgccacgc cggccaggac
     1561 gctgcccacc gcgccgagca cgcgccgcgc gcgcagcagc aacgtgatgc gcggatcggc
     1621 aatcctggac attgcgcctt cctcctgcaa acccgcaaac gcgaacgccg cgacaagtcg
     1681 cggcgtccac ggaatcaacg ttggcagtcc aggcggaccg cctggatcac ttgctcttca
     1741 acacccagta cacgtcgaat tcgccgttct cggtgaacgc accggcgacg cccgacttgt
     1801 agctctcgta gggacgatcg accacttcgt aaccagaggt caacgcccag gcgcgcagcg
     1861 cattgcggac attgtccagc tcggccatgt acccggtgta cgccgcggtg gcgaccttcg
     1921 ccgccggggt ctgcacgtgc tgcaccggac cctgcagggc cggcttgccg accgcgctgc
     1981 catccttctt gcgcacggcc tgggccacgt cgaagttgta ggtctcgcga ccgagttcgg
     2041 tgctgatgat gcgcagcggg ccgacggctt ccaggccgtt ggcttcgatc acgcgcttca
     2101 gccattccgc gttggcgctg atcgaggcct ggatctgcga attgccacgc tcgacgttgc
     2161 cggcgttgac gaacaggatg tcctcgaccg ggcgatccac gaccttcagg ccggtcatct
     2221 tgctgccgga caccgcgtag tcgacgttcg ggaccgacgc cagcatgccg acgatgcgcg
     2281 acaggcccag cttcatgtcg tcgccgacgt ggcggctgac gtacatgccg gcgtagcgac
     2341 ccagcaggtt ccacccgtag tcaacgtcgt agctctgggt gatctcgacg ttgcgattgt
     2401 tgcgaccggt cggcttgaag ctgaagctgg tgcgcttgtt gccgccgcgc tcctcgttca
     2461 cgatcttgta gctgaccttc tcgcgcggga cgctctcggt gatttcccag gagcccttgc
     2521 ccaagccttc ttccttcgac acgtagtcga ggcgggcacc gacgccgctg tccgggcccg
     2581 acagggtgag ctgcacgcgc ggatcgcgca ggatcagcgg gttccagtcc ttgaagcggc
     2641 gcaggctgtt caggctgtcg aacgcgatgg tcagcttgcg gttggtctcg accttctcga
     2701 ccaggtggcg cgaggacggc agcaccaacg ccaccaccag gaacagcccc gccacgatcg
     2761 cgagggaaat caggatctcg agcaaacggg tcattcaggt ctctccggga aggccgctcg
     2821 cggacatgca gccgcgcaga cccgccatcg taccaaaccg ttgccggcgt gggcgagcgc
     2881 caacacgccc gaatcgcgac cgatgcctcc attcgcgtgc gcacggagtc ctgccggccg
     2941 agcgccgcca ggcaacacct tggccgcgtc gggcccatcg caattcgaac gccttcagca
     3001 ccccacgcgt gcggtcgcga acgacgagct tggacagggc gaacggccgt ggcttgcgcg
     3061 ccaccggcgc gcgcggccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     3181 tcgaacgcct tcaggacggc acgggtgcgg tcgcgaacac cgagcttgga cagggcgaac
     3241 ggccgtggct tgcgcgccac cggcgcgcgc ggccgtgagc acccggtgcg ctacaccggg
     3301 ctgggcatcc tgtcgatcag accagttgca actcgaacgc cttcaggacg gcacgggtgc
     3361 ggtcgcgaac accgagcttg gacaggatgt tggagacgtg gttcttgatg gtgccctcgg
     3421 ccacgcccag cgaattggcg atctccttgt tggagaagcc gctggccatc aggcgcagga
     3481 tctcggtctc gcggtcggtg agcggatccg ggcggtccag gctgacgaac tcgttgcgca
     3541 tgtgctccag cccggacagc aggcgctggg tcaccgccgg ctgcaccagc gagccgccgg
     3601 ccgcgaccat ctggatcgcg ttgacaagct gctccagcga gacgtccttc agcaggtagc
     3661 ccttggcccc ggccttgatc ccggccagca ccagcgcatc gtcgtcgaag gtggtgagga
     3721 tgatggtggg cggcaggatg ccggcccggc tcatggcctg cagggattcg aggccggaca
     3781 tcaccggcat gcgcatgtcc atcagcacca cgtccggggc gacctggggg atctgctcca
     3841 ccgcctggcg accgtcggag gcttcggcga ccacttcgat cccctccgcc agggcaagca
     3901 gggagcggat gccctggcgt accagggtct ggtcgtcgac caggaagacc cggatcgggg
     3961 cggcattgct cggggcagga tgggtcatgc ggcgactcct tggggcaagc cggtcgccgc
     4021 attcggcgcc accggcaagg tcatggtgag ttggaacccg tggccaccgc gggtcgcgat
     4081 ctcgagccgg ccatcctgct gccgcaggcg ctcgtgcagg ccgcgcaagc cgttgcccgg
     4141 ctggacctgg tcgctaccgc ggccatcgtc gcggatctgc aggatgacgt ccgggccttc
     4201 cctgcgcgca ctcagccaca ggcggctggc cccggcgtgg cgcaccacgt tggtgatcgc
     4261 ctcctgcgcg caacgcaaca ggatgtgcgc acgctccggg tcgtccaccg tgagtggctg
     4321 ctcgatgtcc atctcgatgg cgagcttcgg cacgttctcg gccaagggac ggagtgcggc
     4381 gccgaggtcg atcgccccgc tttcgcgcaa ttggctgacg gcctcgcgca catcggtcag
     4441 caacaggcgg gccagcgtgt gcgcctgctg gacatgctcc ttgacccgcc cctcggacag
     4501 gtgcccggcc acttccaggt tcaggctcag cgcggtcagg tgatgcccga gcaggtcatg
     4561 cagctcgcgc gaaatccggg tgcgctcgtt gacgcgcgcg ctgtcggcca gcaacgcgcg
     4621 ggtggcgcgc agttccgcgt tgagccggcg ctggtcctcg cgcgcctgca tttgctgcag
     4681 cgcgacaagg ctggtgatga agacgaagcc ggagaagccc gcgtacagca ccgattgcat
     4741 caccgcttcc agtggcggaa atcccagcca gcggatgaac accggtgcca ccgccaactg
     4801 gctggcgagc aaccacgccg ccccgagcgg caacggcagc agccagggca gcacgcacgc
     4861 cgcgaccatc aacagcacgc tgcccagccc cgacccgttg aaatagctga ccgccagcgc
     4921 ggccagggtc agcaccacca gcaggatgta gtcgccgacg ctgcgcgagc gactgcccag
     4981 cccgcgggtg agccatgcat aactcaggcc gaaggcgaga taggccaacc agccctgcca
     5041 gggcatcggc cgcagcgcga gctcctcgtc gccggcggtc cccagcagcg ggcccaccca
     5101 cgagtagacc agcggcaggc ccaccatcgc ccaggtaaac aggccggcgt aacgcagcag
     5161 gcgggtgtgc gagattcggg cgagcatgca ctcttatacc gcctcgcccc gctgcgtgcc
     5221 tgcaccgaag gtcatggccg cgaccggcgg cccatgcgat aattcccctt gatgccagcg
     5281 cctgctggcc acccgctgca acgccatccg gagccaagga atgtcgatcg ccatccgcga
     5341 cgtgcgcgag cacgagctgg attccgtcct cgccctcaac aacgccgcgg gccctgcgat
     5401 ccttcccctc gatgccgcca ggcttcgcca cctgttcgac tgcgccgaac atttccgcgt
     5461 ggccgagcgc gatggcgcgc tggccggctt cctgatcggc ttcggcgcgc atgcgcgcca
     5521 cgacagcagc aactttgcat ggttcggcga acgctaccct gatttcttct acatcgaccg
     5581 cgtggcggtg gccagtcgcc gccgtggcgg cggcgtcggc cgcgcgctgt atgccgatgc
     5641 gcagagctat gccgaactgc gttatccgca actggcctgc gaggtgttcc tgcagcaggg
     5701 caacgaccac gcgctgctgt tccacggcag cttcggcttc cgcgaggtcg gccagcacgt
     5761 catgcccgga catgacatcc gcgcggcgat gctgatgaag ggcctgtgca gttatgcctg
     5821 ggtgcgagac acctatggcg acgccctgcc gggcgaaccc tggctgccgc aaccgcgcgc
     5881 gctggcccag gcgtcgcgcc ctaccgggac atgcgcgtga gcgcggtggt cgattacgcc
     5941 caggccggcg agctgaagtt cggccaggtc ggcatcgcca acctgcgcgt gcgcaccctg
     6001 gacgtggcgc agctggccgc ggaaatgcgc gaccgcgtgc agcgcgcgcc gaatttgttc
     6061 gcgcgcgcgg ccgtggtgct ggatttcggc ggcctgagcc aggtgcctgc ggtcgacgag
     6121 gcccgcgcac tgctcgaagg cctgcgcgat gccggcgtgc tgccggtcgc gctggcttac
     6181 ggcacgaagg agatcgacgc gctgtcgcag cagctcggcc tgccc
//
LOCUS       DAOOCX010000131         6097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_12144_length_6097_cov_3.867925, whole genome shotgun sequence.
ACCESSION   DAOOCX010000131 DAOOCX010000000
VERSION     DAOOCX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12144_length_6097_cov_3.867925"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PK399_10390"
     CDS             <1..362
                     /locus_tag="PK399_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13567.1"
                     /translation="AQQEPDPLWKAYALAMAYWANGDRVRSDAELQVLIRDNPKGAAT
                     QIAGLYAQRDDEAAMFKWLQVARDAGDPGIVEIRYMPFVSRYAEDPRFIALLRELDLV
                     QDTAKAPAPKPAGGNMP"
     gene            complement(381..863)
                     /gene="ybeY"
                     /locus_tag="PK399_10395"
     CDS             complement(381..863)
                     /gene="ybeY"
                     /locus_tag="PK399_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008571323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPW13568.1"
                     /translation="MTKGPIRLDVAVGYAAPRAGIPAAVSFRKWVAAALAGRIREADL
                     AIRIVDEKEGRSLNHHYRGKDYATNVLSFPAELPEGLPKGVKLPLLGDLVLCAPVIAR
                     EAAEQGKPLLAHYAHLTVHGTLHLLGWDHEDAREAEAMEQLEREILAGMGLPDPYRES
                     "
     gene            complement(865..1857)
                     /locus_tag="PK399_10400"
     CDS             complement(865..1857)
                     /locus_tag="PK399_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPW13569.1"
                     /translation="MQNQRDFTLEPDDIERLANMTGPFDAHLRLVELRLGVEIANRGN
                     VFRVDGRDADAVARAERLLRQLYDDAAGEALDEHAIHLRLGAFADGDEAPAASDHPHA
                     SQEIAVRVKRGTLRGRGDNQRKYLQAIAGHDINFGVGPAGTGKTFLAVAMAVDALNQS
                     RVQRVILVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYEMLGVEKVARLLEKNVI
                     EIAPLAYMRGRTLNDAFVILDEAQNTTIEQMKMFLTRLGFGSTAVITGDMTQTDLPKH
                     VKSGLRDATEVLRGVDGVSFTFFEARDVVRHPLVARIVNAYEARDARDAASGAT"
     gene            1951..2670
                     /locus_tag="PK399_10405"
     CDS             1951..2670
                     /locus_tag="PK399_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW13570.1"
                     /translation="MTLSTPAKPRIRTPAPPRKRSPGRPSGKRTPADAAAVRMQLLDA
                     AIACFVRKGIAATSLREIAAEAHVTPALLHYYFGDKAQLQAAVVDEKLLPALAQLREP
                     LEQAGGDVAALVAGFVAGIGRIAIAHPWLPPLWVREVLCEGGALRDVLFEHVGPAIPR
                     MMATRFAAAQADGGLNPDLDPRLLMVSLVGLTLFPIAGAPVWRRLFAADPSAGAEPSL
                     DFETLRRHTLALLDRGLELKA"
     gene            2667..3632
                     /locus_tag="PK399_10410"
     CDS             2667..3632
                     /locus_tag="PK399_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPW13571.1"
                     /translation="MNRIALTRCGVCALAFVATLLAGCEKTPPRVVGTLEWDRITLPA
                     PAAERIVAIEVKEGQQVVAGARLLQLEVTRTRSQLDAVQAQARQSREALAELQAGPRP
                     ERIAQARATLSAAQAQATDARAYYERLRPLGQRQLVAAASVDSARAAAANAEGQVRAA
                     QALLDELLHGTRKEQVAQGEAAGDAARAQEAVQAATLGKLDVVAPRAARVDSLPYKLG
                     DQAPVGSPLAILLVGATPYARVYVPEPLRAGVHVGDAASVSVAGAAPVRGTVRMIRSE
                     PVFTPYYALVGEDASRLSYLAEIQLGNDAAKLPAGLPVQVEFGGR"
     gene            3670..4599
                     /locus_tag="PK399_10415"
     CDS             3670..4599
                     /locus_tag="PK399_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW13572.1"
                     /translation="MRARGVTKRFGALVAVDHVDLTVPKASVYGFLGPNGSGKSTTIR
                     MLCGLLTPNEGAIEVLGLRIPEQAEALRLRIGYMTQKFSLFEDLTVRENLEFLAAVQG
                     LDRTRAKQRIDELVEAYDFADRQKQLAGTMSGGQKQRLALAGAVIHKPELLFLDEPTS
                     AVDPESRRDFWEKLFDLADAGTTLLVSTHYMDEAERCHRIAILDHGHLVADGSPDELT
                     RALDGRSFEVHASEPRRAQRALVGLDGVLSVAQIGNQLRVLVREGVAADASLAQALRQ
                     AGVDADVAASMPNLEDVFVSATHGAKAAMGKGA"
     gene            4596..5705
                     /locus_tag="PK399_10420"
     CDS             4596..5705
                     /locus_tag="PK399_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW13573.1"
                     /translation="MKWGRLWAVMLKELRQMRRDRVTLVMIVGIPVLQLLLFGYAINM
                     NLRGLGAGIADQANSAGSRALVMDMLATGVIRPTLTVDTPQQLMEALRRGEISVGVVV
                     PPDFERRRLDGREAVQVLVDGSDSVVQSAAVQIAQTPLDGSRALVAGTGAREVTPQQI
                     GIVSFYNPERRSAVNIVPGLIGVILTMTMVMFTAIAIVRERERGNMELLIATPVSSGE
                     LMIGKVLPYVAIGLVQVTLVLALGSWLFQVPIRGSLADVYVASLLLVVCNLSLGLMLS
                     TLAKSQFQAMQMTMFVFLPSMLLSGFMFPFAGMPQIVQWFAEVLPLTHFLRLVRGIML
                     RGADIGELWPEVAAMAAFTAVMMTVAILRFRKRLD"
     gene            5767..>6097
                     /locus_tag="PK399_10425"
     CDS             5767..>6097
                     /locus_tag="PK399_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPW13574.1"
                     /translation="MRKQGWSLAIALAVALAPTDGLAAEGETLVTAARIHTMDVAHPS
                     ASAMVYDKGGRILAVGHAEALAKQFPQARRKDLGKATLVPGLIDAHAHVGGLGLAMIT
                     ADLVGTRD"
BASE COUNT          920 a         1985 c         2189 g         1003 t
ORIGIN      
        1 tcgcgcagca ggagccggat ccgctgtgga aggcgtatgc actggcgatg gcgtactggg
       61 ccaacggcga tcgcgtccgc tccgacgccg aattgcaggt gttgatccgc gacaacccca
      121 agggcgcggc cacccagatc gccgggctgt acgcgcagcg cgacgacgag gcggcgatgt
      181 tcaagtggct gcaggtcgca cgcgatgccg gcgacccggg gatcgtggag attcgctaca
      241 tgccgttcgt gtcgcgctac gcggaagacc cgcgcttcat cgcattgctg cgcgaactcg
      301 acctggtgca ggacaccgcg aaagcgcctg cgcccaagcc tgcgggcggc aacatgccct
      361 gatcgcaggc gacgccgggc tcaggattcc cgatagggat ccggcaggcc catgccggcc
      421 aggatctcgc gttccaactg ttccatcgcc tcggcttcgc gcgcgtcctc gtggtcccag
      481 ccgagcaggt gcagggtccc gtgcacggtc aggtgcgcgt agtgcgcgag cagcggcttg
      541 ccttgttccg cggcctcacg cgcgatcacc ggcgcgcaca acaccaggtc gccgagcagc
      601 ggcagcttca cgcccttcgg cagtccttcc ggcaactccg ccgggaagct gagcacgttg
      661 gtggcgtaat ccttgccgcg gtaatggtgg ttgagcgagc ggccttcctt ctcgtcgacg
      721 atccggatcg cgaggtcggc ctcgcggatg cgcccggcca gcgcggccgc cacccacttg
      781 cggaaactca ccgccgcggg aatgccggcg cggggcgcgg cgtaacccac ggcgacatcg
      841 aggcggatcg ggcccttggt catgtcaggt cgctccgctc gccgcatcgc gcgcgtcgcg
      901 ggcctcgtag gcgttgacga tgcgcgccac cagcggatgc cggaccacgt cgcgggcctc
      961 gaagaaggtg aagctgacgc catcgacgcc gcgcagtacc tcggtggcat cgcgcaggcc
     1021 ggacttcacg tgtttcggca agtcggtctg ggtcatgtcg ccggtgatca ccgcggtgct
     1081 gccgaagcca aggcgggtca ggaacatctt catctgctcg atggtggtgt tctgcgcctc
     1141 gtcgaggatc acgaaggcgt cgttgagcgt gcgtccgcgc atgtaggcga gcggggcgat
     1201 ctcgatgacg ttcttttcca gcaggcgcgc cactttctcg acgccgagca tttcgtaaag
     1261 cgcgtcgtac agcgggcgca aatacggatc gactttctgg gtcaggtcgc cgggcaggaa
     1321 gccgagcttc tcgcccgcct ccaccgccgg gcgcaccagg atcacccgct gcacgcgcga
     1381 ctggttcagc gcgtccaccg ccatcgccac cgccaggaag gtcttgccgg tgccggcggg
     1441 accgacgccg aagttgatgt cgtggccggc gatcgcctgc aggtacttgc gctggttgtc
     1501 gccgcgtccg cggagggtgc cgcgcttgac ccgtaccgcg atctcctgcg acgcatgcgg
     1561 atgatccgac gcggcaggcg cctcgtcgcc atcggcgaag gcccccaggc gcaggtggat
     1621 ggcgtgttcg tccagcgcct cgccggcggc atcgtcgtac aactggcgga gcaggcgttc
     1681 ggcgcgcgcg acggcgtcgg catcgcgacc gtcgacgcgg aacacgttgc cgcggttggc
     1741 gatctccacg cccagtcgca gttcgaccag gcgcaggtgg gcatcgaacg gaccggtcat
     1801 gttggccaag cgttcgatgt cgtcgggttc gagggtgaaa tcgcgttggt tctgcatggg
     1861 aaaagggtag cgcgcggact gctcccatgc atcgacgccg cgtccacatc atgagttgat
     1921 atttaattaa atatgaaatt aaatattgac atgacactgt cgacgcctgc caagccacgc
     1981 atccgcacgc cggccccacc gcgcaagcgg tcccccgggc gtccatccgg gaaacgcacg
     2041 ccggccgatg ccgctgccgt gcgaatgcaa ctgctggatg cggcgatcgc ctgtttcgtg
     2101 cgcaagggca tcgccgccac ctcgttgcgc gagatcgccg cggaagcgca tgtcacgccg
     2161 gcgttgctcc actactactt cggcgacaag gcgcagctgc aggccgcggt ggtcgacgag
     2221 aagctgctgc cggcgttggc gcagctgcgc gaaccgctgg agcaggccgg cggcgatgtc
     2281 gcggcgctgg tcgcgggctt cgtcgccggc atcggtcgca tcgcgatcgc ccatccgtgg
     2341 ctgccgccct tgtgggtgcg ggaggtcttg tgcgaaggcg gtgcattgcg cgacgtgctc
     2401 ttcgaacacg tcggccccgc gatcccgcgg atgatggcca cgcgattcgc cgcggcacag
     2461 gcggatggcg ggctcaaccc ggatctggat ccgcgcctgc tgatggtgtc gctggtcggc
     2521 ctgaccctgt tcccgatcgc cggtgcgccg gtgtggcgac gattgttcgc agccgatcct
     2581 tcggcgggcg cggaaccgtc cctcgatttc gaaaccctgc gtcggcatac gctggccctg
     2641 ctcgatcgtg gtctggagct gaaggcatga acaggatcgc cttgacccgc tgcggcgtct
     2701 gcgcgcttgc cttcgtggcc acgttgctgg cgggttgcga aaagacaccg ccacgggtgg
     2761 tgggtacgct ggaatgggac cggatcaccc tgccggcgcc ggcggcggag aggatcgtcg
     2821 cgatcgaggt gaaggagggt cagcaagtgg tcgcaggcgc gcgcctgctg caactggagg
     2881 tgacccggac ccgttcgcaa ctggacgcgg tgcaggcgca ggcaaggcaa agccgcgagg
     2941 cgctggcgga gttgcaggcc gggccgcgtc ccgaacggat cgcgcaggcg cgtgccacgc
     3001 tgtccgccgc gcaggcgcag gccaccgatg cgcgtgcgta ctacgagcga ctgcggccgc
     3061 tcgggcaacg ccagttggtc gccgccgcca gcgtcgacag tgcgcgcgcc gctgcggcca
     3121 atgccgaagg ccaggtgcgc gctgcgcaag cgttgctcga cgaattgctg cacggcaccc
     3181 gcaaggaaca ggtcgcacag ggcgaagccg ccggcgacgc ggcgcgcgcg caggaagccg
     3241 tgcaggcggc gacgctgggc aagctggacg tggtggcgcc gcgcgcggcg cgcgtggaca
     3301 gtcttccgta caagcttggc gaccaggcgc cggtgggttc gccgctggcc atcctgctgg
     3361 tcggcgccac gccgtatgcg cgcgtctacg tgcccgagcc gctgcgtgcc ggcgtgcatg
     3421 tcggcgatgc cgccagcgtc agcgtggccg gtgccgcacc cgtgcgcggt accgtgcgca
     3481 tgatccgcag cgagccggtg ttcaccccgt actacgcgct ggtgggcgaa gacgcatcgc
     3541 gcctcagtta cctcgcggaa atccagctcg gcaacgatgc cgcgaaattg cccgcgggcc
     3601 tgcccgtgca ggtggagttc ggtggtcgct gacgcgcagg cgatcgcctt ttccggcgcc
     3661 gcgccggcga tccgcgcgcg cggcgtgacc aagcgcttcg gtgcgctggt cgccgtggat
     3721 catgtcgacc tgaccgtgcc gaaggcgagt gtgtacggct tcctcggccc gaacggctcg
     3781 ggcaagtcga ccaccatccg catgctgtgc gggttgctca cgccgaacga aggcgcgatc
     3841 gaggtgctgg gcctgcgcat tcccgagcag gccgaggccc tgcgcctgcg catcggctac
     3901 atgacccaga aattctccct gttcgaggac ctcaccgtgc gcgagaacct ggagttcctg
     3961 gccgcggtgc aggggctcga ccggacgcgc gcgaagcagc ggatcgatga gctggtcgaa
     4021 gcctacgatt tcgccgatcg ccagaagcaa ctggccggga ccatgagcgg tgggcagaag
     4081 cagcggctgg cattggcggg cgcggtgatc cacaagcccg agttgttgtt cctggacgaa
     4141 ccgaccagcg cggtggaccc ggaatcgcgc cgcgatttct gggagaagct gttcgacctg
     4201 gccgatgcgg gcaccaccct gctggtgtcg acgcactaca tggacgaggc cgagcgctgc
     4261 caccgcatcg ccatcctcga ccacgggcat ctggtcgccg acggctcgcc ggatgagctc
     4321 acccgagcgc tcgacgggcg cagcttcgaa gtgcacgcat ccgagccgcg gcgtgcgcaa
     4381 cgggcgctgg tcggcctgga cggtgtgctg agcgtggcgc agatcggcaa ccagttgcgc
     4441 gtgcttgtgc gcgagggtgt cgcggccgac gcgtcgctcg cgcaggcatt gcgccaggcc
     4501 ggcgtcgatg ccgatgtcgc cgcatcgatg cccaacctcg aggacgtgtt cgtctccgcc
     4561 acccacggcg cgaaggctgc aatggggaag ggcgcatgaa gtggggcagg ctgtgggcgg
     4621 taatgctcaa ggagctgcgg cagatgcggc gcgaccgcgt caccctggtg atgatcgtgg
     4681 gcatcccggt gctccagctg ttgctgttcg gctatgcgat caacatgaac ctgcgcggat
     4741 tgggcgcggg catcgcggac caggccaaca gcgcgggttc gcgcgctctg gtgatggaca
     4801 tgctcgccac cggcgtgatc aggccgacgc tgaccgtgga cacgccgcaa cagttgatgg
     4861 aagcgctgcg ccgcggcgag atcagcgtgg gcgtggtggt gccgccggac ttcgagcggc
     4921 gtcggctgga cggccgcgag gccgtgcagg tgctggtgga tggcagcgac tcggtcgtgc
     4981 agagcgcggc ggtccagatc gcgcagacgc cgctggatgg cagccgcgcg ctcgtcgccg
     5041 gcaccggcgc ccgcgaggtg acgccgcagc agatcggcat cgtcagcttc tacaaccccg
     5101 aacggcgttc ggcggtcaac atcgtgcccg gcctgatcgg ggtgatcctg accatgacca
     5161 tggtgatgtt caccgcgatc gcgatcgtgc gcgaacgcga gcgcggcaac atggagttgt
     5221 tgatcgcgac gccggtcagc agcggcgaat tgatgatcgg caaggtgctg ccctacgtcg
     5281 ccatcggcct ggtccaggtc accctggtgc tggcgctggg aagctggttg ttccaggtac
     5341 ccatcagggg gtcgctggcc gacgtctacg tcgcctcgtt gctgttggtg gtgtgcaacc
     5401 tcagcctggg gctgatgctg tccacgctgg cgaagtcgca gttccaggcc atgcagatga
     5461 cgatgttcgt gttcctgccg tcgatgctgc tgtccggctt catgttcccg ttcgccggca
     5521 tgccgcagat cgtgcagtgg ttcgccgaag tgttgccgct gacccatttc ctgcgcctgg
     5581 tccgcggcat catgttgcgt ggcgccgaca tcggcgagct gtggccggaa gtggcggcga
     5641 tggccgcgtt caccgcggtg atgatgaccg tggcgatcct gcgcttccgc aagcggctcg
     5701 actgaccggc tcgcgcggcg cggcgcggtt ccgtagagtc ggcgtaccgg ggatggaggg
     5761 ggtgcgatgc gcaagcaagg ttggtcactg gcgattgcac tggcggtcgc gttggcgccg
     5821 acggatggat tggcggccga gggcgagacc ctggtgactg ccgcgcgcat ccacacgatg
     5881 gatgtcgcgc acccgagcgc atcggcgatg gtctacgaca agggcgggcg catcctggcg
     5941 gtcggccacg ccgaggcatt ggcgaaacag ttcccgcagg cacgtcgcaa ggacctcggc
     6001 aaggcgacgc tggtgcccgg cctgatcgat gcgcacgcgc acgtcggtgg cttggggctg
     6061 gcgatgatca cggcggacct ggtcggcacc cgcgaca
//
LOCUS       DAOOCX010000132         6094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_12152_length_6094_cov_8.668323, whole genome shotgun sequence.
ACCESSION   DAOOCX010000132 DAOOCX010000000
VERSION     DAOOCX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12152_length_6094_cov_8.668323"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1202
                     /locus_tag="PK399_10430"
     CDS             96..1202
                     /locus_tag="PK399_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HPW13575.1"
                     /translation="MLLLTFLGGLALLLLGADLLVRGASRLALALGLTPIVVGLTVVS
                     IGTSAPELAISVGGALAGTPDLALGNIVGSNIANVLLILGLVALVTPLVVHRQLVWLD
                     VPLMIGASLLVFAMALDGRIARWEGGVLVAAAIAYILFLVGMARRHPEQVPEDPQLGE
                     VDADTEARPGTLRQLLLMAAGLALLVAGARLLVQAATAIAGALGLSDLVIGLTVVAIG
                     TSLPEIATSLLSSLRGQRDLAVGNIVGSNIFNLLLVLGGTALVAGDGIPVAPTALRFD
                     LPVMTAVAVACLPIFFVGHCIRRWEGALFVGYYVAYTAYLLMAASSHASLDDFRLVMQ
                     AVVMPLTLVTLSVVTWRHVRSRAALPPNTPDGSP"
     gene            1199..2497
                     /gene="purD"
                     /locus_tag="PK399_10435"
     CDS             1199..2497
                     /gene="purD"
                     /locus_tag="PK399_10435"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009573931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPW13576.1"
                     /translation="MKLLVIGSGGREHALAWKLAQSPRVSEVLVAPGNAGTATEAKCR
                     NVAVKATDIDGLLALVEREGIALTLVGPEVPLVAGVVDRFRAAGHRIFGPTAAAAQLE
                     GSKAYAKDFLQRHGIPTAFYEVHTDVEAALAFVRAKGAPIVVKADGLAAGKGVIVAMS
                     LDEAEAAVRDMLDGNAFGDAGARVVIEEFLDGEEASFISMVDGKTALPMATSQDHKRV
                     GDGDTGPNTGGMGAYSPAPVVTPEVHARVMREVVNPTVQGMIADGIPFTGFLYAGLMI
                     DAHGAPKVIEFNVRFGDPETQPVMLRLQSDLVDLLDAAIDGQLHATEAQWDPRPSLGV
                     VMAAKPYPEAPITGDVISGLDTVPGTAKVFHAGTALDADGRVVSAGGRVLCVAALGSS
                     VSDAQRNAYAGVDAVSWANEFHRNDIGWRAIAREQSERGG"
     gene            2502..3641
                     /locus_tag="PK399_10440"
     CDS             2502..3641
                     /locus_tag="PK399_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW13577.1"
                     /translation="MRERVEPFRAMAIGQLARALEAQGQSIIHMEFGQPSTGAPAPAL
                     AAVREALDHDPPGYWESLPLRQRIARLYRERHGLEIDPARILLTCGASPALLLALLTR
                     FEAGDCIAYATPGYVAYRNTTIAAHMQPVAIACGPDVGYQLTADAIAALDPAPAGIII
                     ASPANPTGTIIDADALRAIADTCRQRGITIVSDEIYHGLHYGDAAHSILEFADDAFVV
                     NSFSKYWSMVDWRLGWLLVPTMHIDAARALAGNLFLTPPSPSQQAALVAMDCSDELQA
                     HQAMYARNRALLLEALPTLGLANIAPPDGAFYLWADVGHLTDDSMAFCTRLLQETGVA
                     LAPGVDFDPVDGHRFIRFSFAPDTARIEEALRRLTPWFAAQPRIG"
     gene            complement(3645..4763)
                     /locus_tag="PK399_10445"
     CDS             complement(3645..4763)
                     /locus_tag="PK399_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HPW13578.1"
                     /translation="MSQLFSPLAIGPLSLPNRIVVAPMCQYSSIDGEASDWHVQHWSN
                     LALSGAGLVIVEATAVEPRGRISWADLGLYDDATEAAFANALATVRRWSPAKMGVQLA
                     HAGRKASTLKPWEGGGGPIPADDARGWTTRAPSALPYAETDPVPEALDDAGIAEIIQA
                     FTDSAIRAVRLGLDLIEIHAAHGYLLHEFVSPLSNQRSDAYGGSLENRMRLVLQVFDA
                     IKSVVPESMALGIRISATDWVEGGWDLEQSIALAKALDARGCHFIHVSSGGLHPAQKI
                     AAGPGYQLPFAEAIKREVAMPVIAVGMITEPRQAEAVLVDGQADAVALARAMLFDPRW
                     PWRAAAELGAQVASPPQYLRSAPHGQSKLLQIATGSAG"
     gene            complement(4850..5431)
                     /locus_tag="PK399_10450"
     CDS             complement(4850..5431)
                     /locus_tag="PK399_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HPW13579.1"
                     /translation="MLSMLAAPAAFARELKVLVMGDSLSAAYGLRPEQGWVTLADAKS
                     ANYTFHNASVSGETTAGGRSRFAAELQRAKPDIVIIELGANDGLRGLPLQQMGANLGW
                     MIGASKAAGARVLLVGLQLPPNFGSYATDFQRSYMLISQRLGTGLIPHFLAPLGIERK
                     WFQADNLHPVADAQPLLATQVLKALDTLPASSR"
     gene            5430..>6094
                     /locus_tag="PK399_10455"
     CDS             5430..>6094
                     /locus_tag="PK399_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW13580.1"
                     /translation="MEQTPSTHARIALDIDSTPGPIAADASTPARGGLRTRDVGKVVA
                     LPENDLTILDGISLDIAPGERVAIVGSSGSGKTTLLSLLAGLDLPSRGEVWLDGVPIH
                     AADEDARAAVRAAAVGFVFQNFQLMPAMSALDNVALPLELQGRPGTDAARALLQQVGL
                     GERLHHLPRQLSGGEQQRVAIARAFVAQPRILFADEPTGNLDRRTGIAIEDLLFDIDS
                     ARRT"
BASE COUNT          905 a         2151 c         2062 g          976 t
ORIGIN      
        1 tgccgaagtg atcgccgccg ccgacgaaca cggcctggcg atggtgttca ccggcatccg
       61 ccacttccgc cactgaccgg cgaaggcgcg ccacgatgtt gctcctgacg ttcctgggcg
      121 ggctggccct gctcctgctg ggcgcggacc tgctggtacg gggcgcctcg cgcctggcgc
      181 tcgcgctggg cctgacgccg atcgtggtcg gtctgactgt ggtgtcgatc ggcaccagcg
      241 caccggaact ggcgatcagt gtgggcggcg cgctggcggg aacccccgac cttgctctgg
      301 gcaacatcgt tggcagcaac atcgccaacg tcctgctgat cctcgggctg gtggcgctgg
      361 tgacgccgtt ggtggtgcac cggcaactgg tgtggctgga cgtcccgctg atgatcggcg
      421 cttcgctgct ggtgttcgcg atggcgctgg atggccgcat cgcgcgctgg gagggcgggg
      481 tgctggtcgc cgccgcgatc gcctacatcc tgttcctggt cgggatggcg cgacggcatc
      541 ccgagcaggt gcccgaagac ccgcaactgg gcgaggtcga tgccgatacc gaggcgcggc
      601 cgggcaccct gcgccagttg ctgctgatgg ccgcgggcct tgcgctgctt gtggccggcg
      661 cgcgcctgct ggtgcaggca gcgacggcga tcgcgggcgc gctggggctc agcgacctgg
      721 tgatcggcct gaccgtggtc gcgatcggca cctcgctgcc ggagatcgcc acctccctgc
      781 tgtcatccct gcgcggccag cgcgaccttg cggtcggcaa catcgtcggc agcaacatct
      841 tcaacctgct gctggtgctc ggcggcaccg cgctggtcgc cggcgacggt atcccggtcg
      901 caccgaccgc cctgcgcttc gacctaccgg tgatgaccgc ggtcgcggtc gcctgccttc
      961 ccatattttt cgtcggccac tgcatccggc gctgggaagg cgcgctgttc gtgggttact
     1021 acgtggccta caccgcctac ctgttgatgg cggcgtccag ccacgcctcg ctggacgact
     1081 tccggctcgt catgcaggcg gtggtcatgc cgctgaccct ggtaaccctg tcggtggtca
     1141 cctggcgcca cgtgcgctcg cgcgcggcac tcccccccaa cacgccggac ggctccccat
     1201 gaaactcctc gtcatcggtt ccggtggccg cgaacacgcg ctggcgtgga agctggcgca
     1261 atcgccgcgc gtctccgaag tcctggtcgc gccgggcaat gccggcaccg cgaccgaagc
     1321 gaagtgccgc aacgtggcgg tcaaggccac cgacatcgac ggcctgctcg cgctggtcga
     1381 gcgcgagggc atcgcgctga cccttgtcgg cccggaagtg ccgctggtgg cgggcgtggt
     1441 cgaccgcttc cgcgctgccg gccatcgcat cttcggaccc accgccgccg ccgcgcagct
     1501 ggaaggcagc aaggcctacg cgaaggactt cctgcaacgg cacggcattc ccaccgcgtt
     1561 ctatgaagtg cataccgatg tcgaggccgc gctcgcgttc gtgcgtgcga aaggcgcgcc
     1621 gatcgtcgtg aaagccgatg gcctggccgc cggcaagggc gtgatcgtgg cgatgtcgct
     1681 cgatgaagct gaagccgcgg tgcgcgacat gctcgatggc aatgccttcg gcgatgccgg
     1741 cgcgcgcgtc gtcatcgagg aattcctcga tggcgaggaa gccagcttca tctcgatggt
     1801 ggacggcaag accgcgctgc cgatggcgac ctcgcaggac cacaagcgcg tgggcgacgg
     1861 cgacaccggc ccgaacaccg gcggcatggg cgcgtactcg ccggcaccgg tggtcacgcc
     1921 cgaggtgcat gcgcgggtga tgcgcgaggt ggtgaacccg accgtgcagg gcatgatcgc
     1981 cgacggtatc ccgttcaccg gtttcctcta cgcgggcctg atgatcgatg cgcatggcgc
     2041 gccgaaggtg atcgagttca acgtccgctt cggcgacccg gaaacccagc cggtgatgct
     2101 gcgcctgcag tccgacctgg tcgacctgct cgatgccgcg atcgatggcc agttgcacgc
     2161 gactgaagcg caatgggacc cgcgcccgtc gctgggcgtg gtgatggcgg cgaagcccta
     2221 tcccgaagcg ccgatcaccg gcgacgtcat ctccggcctc gacacggtgc ctggcaccgc
     2281 caaggtgttc cacgccggca ctgcgcttga cgccgatggt cgcgtggtca gcgccggtgg
     2341 ccgcgtgctc tgcgtcgctg cgctgggcag ttccgtttcc gacgcacaac gcaatgcgta
     2401 tgccggcgtc gatgcggtct catgggcgaa tgaattccat cgcaacgaca tcggctggcg
     2461 cgcgatcgcg cgcgagcagt ccgaacgcgg cggctgaggc catgcgcgag cgcgtcgaac
     2521 cgttccgcgc gatggcgatc ggccagctgg cccgcgcgct ggaagcgcag ggccagtcga
     2581 tcatccacat ggagttcggc cagccctcca ccggcgcgcc ggccccggca ttggcagcgg
     2641 tgcgcgaggc gctggaccac gatccgccgg gctactggga aagcctgccg ctgcgccagc
     2701 gcatcgcgcg gctgtatcgc gaacgccacg gcctcgagat cgatcccgcg cgcatcctgc
     2761 tgacgtgcgg cgcgtcgcct gccctgctgc tcgccttgtt gacgcgcttc gaggccggcg
     2821 actgcatcgc ctatgccacg ccgggttatg tcgcctatcg caacaccacg atcgccgcgc
     2881 acatgcagcc ggtcgcgatc gcatgtggac ccgacgtcgg ctaccagctc accgccgacg
     2941 ccatcgccgc gcttgaccca gcgcctgccg ggatcatcat cgccagcccg gccaatccca
     3001 ccggcacgat catcgatgcc gatgcattgc gcgccatcgc cgacacctgc cggcaacgcg
     3061 gcatcaccat cgtctccgac gagatctacc atggcctgca ctacggcgac gccgcgcatt
     3121 cgatcctcga gttcgccgac gatgccttcg tggtcaacag cttctccaag tactggagca
     3181 tggtcgactg gcggctcggc tggctgctgg tgccgaccat gcacatcgac gcagcgcgtg
     3241 ccttggccgg caacctgttc ctgaccccgc cgtcgcccag ccagcaggcg gcgctggtgg
     3301 cgatggactg cagcgacgaa ctgcaggccc accaggcgat gtacgcgcgc aatcgtgcgc
     3361 tgctgctcga ggcattgccg acgctcggac tggccaacat cgcgccgccc gacggcgcgt
     3421 tctacctctg ggccgacgtc ggccacctga ccgacgacag catggcgttc tgcacgcgcc
     3481 tgttgcagga gaccggcgtt gcgctggcgc cgggcgtgga tttcgacccg gtcgacggcc
     3541 atcgcttcat ccgcttcagc tttgcgccgg acactgcacg catcgaggaa gcgttgcgcc
     3601 gcctgacgcc gtggttcgcg gcgcaacctc ggatcggctg acgctcagcc ggcgctgccg
     3661 gtcgcgatct gcagcaactt cgattgcccg tgtggcgcac tgcgcaggta ctgcggcggc
     3721 gacgcgacct gcgcgcccaa ctcggcggcg gcacgccaag gccagcgcgg atcgaacagc
     3781 atcgcccgtg ccagcgcgac ggcgtcggcc tggccatcga ccagcaccgc ttccgcctgt
     3841 cgcggttcgg tgatcatgcc caccgcgatc accggcatcg ctacctcgcg cttgatcgcc
     3901 tcggcgaacg gcaactggta accgggaccc gccgcgatct tctgcgcggg atgcagcccg
     3961 ccactcgaca cgtggatgaa atggcagccg cgcgcgtcca gggccttggc cagcgcgatg
     4021 ctctgttcga gatcccaacc gccttcgacc cagtcggtgg cggagatgcg gatgcccaac
     4081 gccatcgact ccggcaccac cgacttgatc gcgtcgaaca cctgcaacac cagccgcatg
     4141 cggttctcca gcgacccgcc ataggcatcg ctgcgctggt tgctcaacgg cgacacgaat
     4201 tcgtgcagca gatagccgtg cgcggcatgg atttcgatga gatccaagcc gagtcgcacc
     4261 gcgcggatcg cgctgtcggt gaaggcctgg atgatttcgg cgatgccggc atcgtcgagg
     4321 gcttcgggca ccgggtcggt ttcggcatac ggaagcgcgg aaggcgcccg cgtggtccag
     4381 ccgcgcgcat cgtcggccgg gatcgggccg ccaccgcctt cccagggctt gagcgtcgag
     4441 gccttgcgcc cggcatgggc aagctggacg cccatcttcg ccggcgacca gcgccgcacc
     4501 gtggccaacg cgttcgcgaa cgcggcctcg gtggcgtcgt cgtacaggcc caggtcggcc
     4561 cagctgatcc gcccgcgcgg ctcgaccgcg gtggcttcga cgatcaccag cccggcgccg
     4621 gacagcgcga ggttgctcca atgctgcaca tgccagtcgc tggcttcacc gtcaatcgac
     4681 gagtactggc acatcggcgc caccacgatg cggttgggca gcgacagcgg gccgatggcc
     4741 agcggcgaga agagttggct catgcgggca ctccaaggcg ggccgccact atccggtgcc
     4801 gccggcgtca cgacaacgcc atccgcggaa cagtgacgtg caggccggcc tagcggctcg
     4861 atgctggcaa ggtatcgagt gccttgagca cctgcgtggc caacagcggc tgcgcgtccg
     4921 cgaccgggtg caggttgtcg gcctggaacc atttgcgctc gatgccgagc ggtgccagga
     4981 aatgcgggat caggccggtg ccgaggcgct ggctgatcag catgtagctg cgctggaagt
     5041 cggtggcgta gctgccgaag ttcggcggca gttgcaggcc gaccagcagg acgcgggcac
     5101 ccgcggcttt cgaggcgccg atcatccagc ccaggttcgc acccatctgc tgcagcggca
     5161 ggccgcgcag gccgtcgttg gcgccgagct cgatgatgac gatgtcgggc ttggcgcgtt
     5221 gcagttccgc ggcgaaacgg ctgcgaccgc ctgccgtggt ttcaccggag acgctggcgt
     5281 tgtggaaggt gtagttcgcg gatttcgcat cggccagcgt gacccagccc tgctccggcc
     5341 gcaggccata ggcggcggac aatgaatcgc ccatcaccag taccttcagc tcacgcgcga
     5401 aggccgccgg tgccgctagc atcgacagca tggaacagac ccccagcacc cacgcccgca
     5461 tcgccctcga catcgattcc actccaggtc caatcgccgc agacgctagc acgcccgccc
     5521 gtggcggttt gcgaacccgc gacgtgggca aggtggtcgc gctgccggag aacgacctga
     5581 ccatcctcga cggcatcagc ctggacatcg cgccgggcga gcgggtcgcc atcgtcggca
     5641 gttccggttc cggcaagacc acgctgctct cgctgctggc cgggctggac ctgcccagcc
     5701 gcggcgaggt ctggctggat ggcgtgccga tccacgctgc cgatgaggac gcccgcgccg
     5761 cggtgcgtgc cgccgcggtc ggcttcgtgt tccagaattt ccagctgatg ccggcgatga
     5821 gcgcgctcga caacgtcgcg ttgccgctgg aactgcaggg ccggccgggc accgacgcag
     5881 cccgtgcatt gctgcagcag gtcggcctcg gcgaacgcct gcaccacctg ccgcgccagc
     5941 tgtccggggg cgagcagcag cgtgtcgcga tcgcacgcgc cttcgtcgcg cagccgcgca
     6001 tcctgttcgc cgacgaaccc accggaaacc tcgaccgccg caccggcatc gcgatcgagg
     6061 atctgctgtt cgacatcgac agcgcgcgcc gcac
//
LOCUS       DAOOCX010000133         5912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_12686_length_5912_cov_5.860850, whole genome shotgun sequence.
ACCESSION   DAOOCX010000133 DAOOCX010000000
VERSION     DAOOCX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12686_length_5912_cov_5.860850"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PK399_10460"
     CDS             complement(<1..340)
                     /locus_tag="PK399_10460"
                     /inference="COORDINATES: protein motif:HMM:NF023338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HPW13581.1"
                     /translation="MTLRLGYAGLVPFMLGALLLWLVRPDAHPYVAAALSAYAAVIVS
                     FLGGIHWGLGFRAQPADPSRFIWGVVPSLVAWVAVVMPPYAGLVVHGVMLIVCYGVDR
                     RIYPVQAAAAW"
     gene            complement(394..1659)
                     /locus_tag="PK399_10465"
     CDS             complement(394..1659)
                     /locus_tag="PK399_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HPW13582.1"
                     /translation="MIPLLQDFLAFEPTYAEALRRLDAVHPGEYARTRNLLDGAVTGL
                     SPYLTHGVISLRAAAARVLQKHRLGYDDKLMFEFAWREFFHHVWSRCGEQVLADMRPA
                     GLWPGSYATTVPADLREGRTGVPTIDAAVRQLYATGYLHNHARMWLASYAVHLRKVHW
                     RAGADWMVGHLLDGDVPSNHLSWQWVAGTFSNKPYLFNADNVARYATGPAFAGWQGRG
                     TVIDQDYEALDRTARLDGDVGPERGEHAGIVESLLWAAPLPAWVADSTKPLIAAALPK
                     RREIELVTPWSLSEREGGSGAGAGGPWRLGLIHLPAHEAMPWSEARWRWVLRRMGQVT
                     DAVFVGDVMKRPASTQGVPAWPGRDGVFAFRAPMPGYDAALGTLATLRPASRLLPNPE
                     TPCRSFSVFYEAVKRQSRSLQSQLNGELP"
     gene            complement(1656..2387)
                     /locus_tag="PK399_10470"
     CDS             complement(1656..2387)
                     /locus_tag="PK399_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HPW13583.1"
                     /translation="MHLASSGALSLLVLMGLVPAAHAGEAEIRKALGERLPDLGKVDE
                     VTKIPVAGLFEVRIGTDIYYTDENGSYLFEGHLIDTRNQVNLTEERISKLTAIDFATL
                     PLKDAIVWKQGDGSRKIAVFADPNCGYCKKFERDIRQIKDLTIYTFLFPILGGDSPEK
                     SRNIWCAKDRAKTWVDWMVDGATPPRMMGDCDASALQRNAALGRKHKVTGTPALVFED
                     GKRVPGAMKADQIEKQLLASAKKPG"
     gene            complement(2483..3610)
                     /locus_tag="PK399_10475"
     CDS             complement(2483..3610)
                     /locus_tag="PK399_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPW13584.1"
                     /translation="MKPYDVQVRGTGLVGHALALSLARIGLRVALVADPSASNHVARG
                     PDVRAYALNAGSVGLLRGLKVWDALPPHAATAVHEMRVQGDTGAAVEFSSWEQQVGEL
                     AWIADAAVLERELASAVRFSPHITVVGAGDPGAATAALTAICEGKASASREAHRVPLD
                     RRPYGHRAIATRLTSSKPHAGTARQWFRSPDVLALLPFDAPLPEASYALVWSVPDARA
                     DELLAMDAVDFIDALMQATDGAAGHLDLAGERAAWPLMRAQAGAWCGPGWVLVGDAAH
                     VVHPLAGQGLNLGLADVAALTGVLAAREPWRDLGDDKLLRRYARQRLSPTRAMIEITD
                     GLLHLFAHQSPAVRELRNRGLSLVNRLTPIKRWLTARALDS"
     gene            3721..>5912
                     /locus_tag="PK399_10480"
     CDS             3721..>5912
                     /locus_tag="PK399_10480"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HPW13585.1"
                     /translation="MLGLLLVLAAVLTWLVMGFDANRYKGIAIEWMQTHHNRTLTING
                     PIGLSLFPRLQLTLADVSLSEAGRPDEFAGLERAELAVDVLPLLDRELVIGRISARGV
                     RANYLQNALGVRNIDDLLHPEKDPSDPGSIGSKKPPRLDISSIELEDVRLHVKDEVAG
                     VEGDLMVKSLTTGRLRNQAESPVELKLQVDLKAPAVRGELSGDAQLTLDLDTGSVRAR
                     HLDLAFKGDTKDISGIRATVKGSAGWDGSTSAIEATALQISVAATAGGLKIDSSSLGI
                     ARFAYNPAAKGLELTQLKFEMKGAQEAQSLSFNLDWPELAVAGDQLKGSAFTGRLQRG
                     GTAPLDVNFRSAAPTGSFDLLRLPGVEATFASSGAQRKIGGKLTTNLVLRQGQAAGAG
                     KSSAATPASITLDALTLQSLIENPGLQELKIDARGTASASVQRASWTLAGEMNGAAFN
                     TDGQAQLGGAVPKVIAKARFDTLDLNRLLADAPPPAVPAPPPGGKATAAAADTPVDLS
                     GLNGINGQFSLQAGTFAVQRYRVSAARIEATLDRGLLRVTTLQGKIWGGSVDASALAE
                     ASTGRLGVKGAATGIDINGALKDVAAKDWMDGTGRVSMDIEATGRSVNELKSRLQGQL
                     GLQLRDGAIKGINLAKSLRQAKAAIGLKQDAVQKASETEKTDFSELSASFQIADGVAR
                     SKDLDVKSPFLRLGGEGAVDIGKGRIDYLARAAVTGTSKGQDGADLASL"
BASE COUNT         1071 a         2007 c         1998 g          836 t
ORIGIN      
        1 gccaggctgc tgcagcctgc accgggtaga tgcggcggtc gaccccgtag cagacgatca
       61 gcatcacgcc atgcacgacc agtccggcat agggcggcat caccaccgcg acccaggcga
      121 ccagcgaggg cacgacaccc cagatgaaac gagacggatc ggccggttgc gcacggaatc
      181 cgaggcccca gtggatgccg ccgagaaacg agacgatgac agcggcatac gccgacagtg
      241 cggccgcgac gtaagggtgg gcatcgggcc gcacaagcca cagcagcagc gcacccagca
      301 tgaacggcac cagccccgca tagcccagac gcaaggtcac cgcgtcgggc tgattcgtcg
      361 acgcgggtgc gtcaggaaac cgactccgac cactcaagga agctctccgt tcaactgcga
      421 ttgcaacgac ctggattgtc gcttgaccgc ttcatagaac acgctgaacg agcggcaggg
      481 cgtctcggga ttcggcagca ggcgcgacgc ggggcgcaag gtcgcgagcg tgccgagcgc
      541 cgcgtcgtag ccgggcatgg gcgcccgaaa ggcaaagacc ccgtcgcgtc cgggccaggc
      601 gggcacgccc tgggtggacg ccggcctctt catcacgtcg ccgacgaaga ccgcatcggt
      661 gacctgcccc atgcgtcgca gcacccagcg ccagcgtgcc tcgctccacg gcatcgcttc
      721 atgcgccggc aggtggatca ggcccaggcg ccaagggcca ccggcaccgg cgcccgatcc
      781 gccttcacgc tcgctcaggg accacggcgt gaccagttcg atctcgcgcc gcttcggcag
      841 tgcggcagcg atcagcggct tcgtggagtc ggcgacccat gcgggcagag gcgcggccca
      901 caacagcgac tccacgatgc cggcatgctc gccgcgctcg gggccgacat cgccgtccag
      961 acgtgcggtg cggtcgagcg cctcgtagtc ctggtcgatc acggttcccc ggccctgcca
     1021 gcctgcgaag gccgggccag tcgcgtaccg ggccacgttg tccgcgttga acaggtaggg
     1081 cttgttcgag aaggtgcctg ccacccactg ccagctcagg tggttgctcg gcacgtcacc
     1141 gtccaggagg tggccgacca tccagtcggc acccgcacgc cagtgcacct tgcgcaggtg
     1201 caccgcatag ctggccagcc acatgcgggc gtggttgtgg aggtaaccgg tggcgtagag
     1261 ctggcgcacc gccgcatcga tcgtcggcac gccggtgcgg ccctcgcgca gatcggccgg
     1321 caccgtcgtg gcgtagctgc ccggccacag ccccgcaggg cgcatgtcgg ccagcacctg
     1381 ctccccgcag cggctccaga catggtggaa gaactcgcgc cacgcgaatt cgaacatcag
     1441 cttgtcgtca taaccgagcc ggtgcttctg cagcacccgg gcggcggccg cacgcagcga
     1501 gatcacaccg tgcgtcaggt acggagacag gccggtgacg gccccgtcga gcaggttgcg
     1561 ggtgcgcgcg tactcgcccg gatgcaccgc gtcgagacgc cgcagcgcct cggcgtaggt
     1621 cggctcgaag gcgaggaaat cctgcagcag cggaatcaac ccggcttctt cgcggaggcc
     1681 agcaactgct tctcgatctg gtcggccttc atcgcaccgg gcacccgctt gccgtcctcg
     1741 aacaccagcg ctggcgtgcc ggtgaccttg tgcttgcgac caagcgccgc attgcgttgc
     1801 agcgcggagg catcgcagtc acccatcatg cgcggtgggg tcgcgccatc gaccatccag
     1861 tcgacccagg tcttcgcacg gtccttcgcg caccagatgt tgcgcgactt ctcgggcgaa
     1921 tcgccaccga ggatcgggaa caggaaggtg tagatcgtca ggtccttgat ctggcggatg
     1981 tcgcgctcga acttcttgca gtatccgcag ttcggatcgg cgaacaccgc aatcttgcga
     2041 ctgccatcgc cctgcttcca gacgatcgca tccttcagcg gcagggtcgc aaaatcgatg
     2101 gcggtcagct tggagatgcg ctcctcggtc aggttgacct ggttcctggt gtcgatcaga
     2161 tgaccttcga acaggtagct gccgttctcg tcggtgtagt agatgtcggt gccgatgcgg
     2221 acctcgaaca gaccggcgac cgggatcttc gtgacctcgt cgaccttgcc gagatcgggc
     2281 agccgttcgc ccagcgcctt gcgtatctcc gcctctccgg catgtgcagc gggcacgagg
     2341 cccatcaaca ccagcagcga cagcgcacct gaggaagcaa ggtgcaccaa gctgcgatgc
     2401 agcgcacgga aggaaggcaa gggagtgacc atgatgttgg actttcgggt gatcgaagag
     2461 ggcagggcca ggccaggcgg cttcacgaat ccagcgcccg tgcagtcaac cagcgcttga
     2521 tgggcgtgag ccgattgacc agactcagcc cgcggttgcg gagttcccgc acggccggcg
     2581 actgatgcgc aaaaagatgc agcagtccat cggtgatctc gatcatcgcg cgcgttggtg
     2641 acaaacgttg ccgcgcatag cggcgcagca gcttgtcgtc gcccagatcg cgccagggct
     2701 cgcgcgcggc gagcaccccg gtcagcgccg ccacatcggc cagtcccagg ttcaggccct
     2761 ggcccgccag cgggtgcacg acatgcgcgg catcaccgac cagcacccaa cccggcccgc
     2821 accaggcgcc agcctgagca cgcatcagcg gccaggcggc acgctcgcct gccaggtcca
     2881 gatgaccggc ggcgccatcg gtggcctgca tcagggcatc gatgaaatcg acggcatcca
     2941 tcgcgagcaa ctcgtcggcg cgcgcatcgg gcacggacca gaccagcgca tacgaggcct
     3001 ccggcagcgg cgcatcgaaa ggcagcagcg cgagcacatc cggcgagcgg aaccactggc
     3061 gcgcggtgcc cgcatgcggc ttcgagctgg tcaggcgcgt cgctatcgcg cggtgaccgt
     3121 agggacgccg gtcgagcggc acgcgatgcg cctcgcggct ggcggaagcc ttgccttcgc
     3181 agatggcggt gagcgcagca gtcgctgccc ccggatcgcc cgccccgacc acggtgatgt
     3241 gcggcgagaa ccgcaccgca gaagccagtt cgcgttcgag aacggccgca tcggcgatcc
     3301 aggcgagttc accgacttgc tgctcccatg aggaaaactc gacagcagcg ccggtgtctc
     3361 cctgcacgcg catctcgtgc acggccgtgg ccgcatgcgg cggcaacgca tcccacacct
     3421 tgagcccacg tagcaggccg acggatcctg cattcagcgc gtaggcgcgc acgtcggggc
     3481 cgcgcgccac gtggttcgat gccgacgggt cagcgaccaa ggccacacgc agtccgatgc
     3541 gcgccagcga caaggcaagc gcgtggccga ccagaccggt tccacgcacc tgcacgtcat
     3601 agggtttcat cgcggtcgat tgtagggagc cgagtctggc gggccggaac gggacaggca
     3661 caatccgccg ttttcggggg ctttgccgtg tcgatgtgga ttcgccgtgt ggcactcggg
     3721 atgttgggac tgctgctggt gctcgcagcg gtgctcacct ggctggtcat gggcttcgac
     3781 gccaaccggt acaagggcat cgccatcgag tggatgcaga cccaccacaa ccgcacgctg
     3841 acgatcaacg ggccaatcgg cttgtcgctt ttcccgcgcc tgcagctcac gctggccgac
     3901 gtgtccctga gcgaggccgg gcggcccgac gagtttgccg ggctcgaacg cgccgagctc
     3961 gccgttgatg tgctgcccct gctagaccgc gagttggtga tcggacggat ttcggcgcgc
     4021 ggagtacgcg ccaactacct gcagaacgct ctcggcgtgc gcaacatcga tgatctgctg
     4081 caccccgaga aggacccttc ggaccccggg agcatcggca gcaagaagcc gccccggctc
     4141 gacatcagca gcatcgaact ggaagacgtg cgcctgcacg tcaaggacga agtggccggc
     4201 gtcgagggcg atctgatggt gaagtcgctc accaccggtc gcctgcgcaa ccaggccgag
     4261 tcgccggtcg agctgaaatt gcaggtcgac ctgaaggcgc ccgccgtgcg gggcgaactg
     4321 agcggagatg cacaactgac gctcgatctg gacaccggct cggtgcgcgc acgccatctc
     4381 gacctcgcgt tcaagggcga cacgaaggac atcagcggga tccgcgccac cgtgaaaggc
     4441 agcgcgggct gggacggctc cacctcggcg atcgaggcaa cggcgctgca gatcagcgtg
     4501 gccgcgaccg cgggcggcct gaagatcgac agcagctcgc tgggcatcgc gcgcttcgcc
     4561 tacaacccgg cggccaaggg gttggagctc actcagctga aattcgagat gaagggggcc
     4621 caggaagccc agtccttgtc cttcaacctg gactggcccg aactggccgt ggccggtgat
     4681 cagctcaagg gcagcgcctt caccgggcga ctgcagcgag gcggcacggc accgctcgac
     4741 gtgaatttca ggagtgcggc cccgaccggc agtttcgacc tgctgcggct ccccggcgtc
     4801 gaggccacct tcgccagcag cggcgcccag cgcaagatcg gcggcaagct gacaacaaac
     4861 cttgtcttgc gacaggggca agctgccgga gccgggaaga gctccgccgc aacacccgca
     4921 tcgatcacgc tcgatgcgct gacattgcag agcctgatcg agaacccggg gctgcaagaa
     4981 ctgaagatcg acgcacgcgg cacggccagc gcgtcggtgc agcgcgccag ctggacgctg
     5041 gccggggaga tgaacggcgc cgcgttcaac accgacggac aggcccagct gggcggtgcc
     5101 gtgccgaagg tgatcgccaa ggcgcgcttc gacacgctgg acctgaaccg cctgctggcc
     5161 gatgcgcctc caccggccgt ccccgcgccg ccgcccgggg gcaaggcgac tgcggctgcg
     5221 gccgacaccc cggtcgacct gagcggactg aacggcatca acggccagtt cagcctgcag
     5281 gcgggcacct tcgcggtgca gcggtaccgt gtttcggcag cccgcatcga agccacgctg
     5341 gaccgaggtc tgctgcgcgt gaccacgctg cagggaaaga tctggggcgg cagcgtcgat
     5401 gcatcggcgc tggccgaagc atcgaccggg cgcctcggcg tcaagggcgc agccaccggc
     5461 atcgacatca acggcgcgct gaaggacgtg gcggcgaagg actggatgga cggcaccggc
     5521 cgcgtgtcga tggacatcga agccaccggc cgcagcgtca acgagctgaa atcccggctg
     5581 caagggcagc tcgggctgca actgcgcgac ggcgcgatca agggcatcaa cctcgcgaag
     5641 agcctgcgcc aggccaaggc ggccatcgga ctcaaacagg acgcggtgca gaaggccagc
     5701 gagaccgaga agaccgattt cagcgaactg agcgcgagct tccagatcgc ggacggcgtc
     5761 gcccgcagca aggatctgga cgtgaagagt ccgttcctgc gcctgggtgg cgagggcgcg
     5821 gtcgatatcg gcaagggccg catcgactac ctggcgcggg ccgccgtgac cggcacctcg
     5881 aagggtcagg acggcgccga cctggcctcg ct
//
LOCUS       DAOOCX010000134         5888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_12751_length_5888_cov_5.797531, whole genome shotgun sequence.
ACCESSION   DAOOCX010000134 DAOOCX010000000
VERSION     DAOOCX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12751_length_5888_cov_5.797531"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PK399_10485"
     CDS             complement(<1..683)
                     /locus_tag="PK399_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPW13586.1"
                     /translation="MRPDLSLPALRTRYLSGELTPTRLVTELHARIDAEEAALPRNVW
                     IHRLGLDALKRYASALEGRDPATLPLYGIPFAIKDNIDLAGVPTTAACPAFAYTPQRS
                     ATVVQRLIDAGAIPVGKTNLDQFATGLVGVRSPYGACRNSIDPAFVSGGSSSGSAVSV
                     ALGLASFSLGTDTAGSGRVPAAFNNLIGLKPSLGRIPTTGVVPACRTLDAVSVFALTA
                     ADAATVLASA"
     gene            complement(707..4348)
                     /gene="uca"
                     /locus_tag="PK399_10490"
     CDS             complement(707..4348)
                     /gene="uca"
                     /locus_tag="PK399_10490"
                     /EC_number="6.3.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011052894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004847 - urea carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase"
                     /protein_id="HPW13587.1"
                     /translation="MFDKVLIANRGAIACRIIRTLRRLGVGSVAVYSEADAGALHVSM
                     ADESVCIGPAPVASSYLDGARIIEAALATGAKAIHPGYGFLSENAGFVEACEAAGLVF
                     LGPAPQQMRLFGLKHTARALATDSGVPLLPGTGLLSDLGEALARAEQIGYPVMLKSSA
                     GGGGIGMQMCAGADELRSAFDTVRRLAQSNFSDAGVFIERYITDARHIEVQVFGDGAG
                     QVLALGDRDCSLQRRNQKVVEEAPAPHLRPEVRAALHATARRLAQAAHYRSAGTVEFV
                     VDASTQDFYFLEVNARLQVEHGVTEAVTGIDIVEWMVRLGAGEMPALDSLRVTQTGHA
                     VQARLYAEDPFKNFQPATGVLTRVEFPADLRVDTWIERGTEVTAYYDPMLAKLIAHGS
                     DRADAIAKLSKGLAVTHVDGLESNLEYLRAVMSFAPFADGMHTTRSLASFSAPSRAFD
                     VLSPGVQTSVQDWPGRLGYWDVGVPPSGPMDPLALRLANRLLGNEQGAAGLEITVAGP
                     SLRFRCPAVVAVCGAPIELSLDGKAIPMGRAVTVEAGQNLRIGRVGQSGAAGCRAYLA
                     VAGGIDVPEYLGSGSTFTLGQFGGHGGRVLRGGDVLHLGQPVSATPAPISAEQAAMLT
                     PACGTHWDIGVLIGPHAAPDFFTPDDVATFFATDWQVHYNSSRTGVRLIGPKPTWARS
                     DGGEAGLHPSNIHDNAYAIGAIDFTGDMPVILGPDGPSLGGFVCPATVVQAELWKLGQ
                     LSPGNTVRFKPLTLAEARALAAAQDAVLSATAPLANTAPAAAPISRALLSDAAVLHHR
                     AARDDGIEIVYRRAGDAYVLIEFGELKLDLTLRFHVHALMQLLESWRDEGRLAGVIDL
                     TPGIRSLQVHYDPSRIALATLMDALLDAQDGVPATADIEVPSRTVWLPLSWDDAATRL
                     AIEKYMQSVRADAPWCPSNIEFIRRINGLDDIQAVYDTVFEASYMVLGLGDVYLGAPV
                     ATPLDPRHRLVTTKYNPARTWTPENAVGIGGAYLCVYGMEGPGGYQFVGRTVQMWNRW
                     KSTSEFEPGKPWLLRFFDQLRFYPVSADELLKLRSDFVAGRAGLRIQDGTFRLADYQR
                     YLVENGAAISAFKTRQQAAFEAERERWRAAGVAETVEETVDADLDARAAAASAFDGEV
                     ITSEVSGGVWSVLAAKGSAVQAGDTLLVVESMKMEITVLAPRAGVVHEVLCSEGQPVT
                     AGQPLVLLR"
     gene            complement(4423..5097)
                     /locus_tag="PK399_10495"
     CDS             complement(4423..5097)
                     /locus_tag="PK399_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HPW13588.1"
                     /translation="MAATVESPLLPADAVIDEICLAGDPWAKVVKKGQTLRIVDIEGN
                     QAVDTIFYSAADVGERYSLTHTIQAQGALYLTVGSVLMSSEARPMLTVTADTCGRHDT
                     LGGACACESNTVRYALEKRYMHSCRDSFMLALARIDSGSESTAGIPRLTKRDVVSNIN
                     FFMNVPVTPEGKLTFEDGISAPGKYVEMRAEMDVVVLVSNCPQLNNPCNGYNPTPVRF
                     LVWDPA"
     gene            complement(5097..5834)
                     /locus_tag="PK399_10500"
     CDS             complement(5097..5834)
                     /locus_tag="PK399_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HPW13589.1"
                     /translation="MPDRSAPYAQDLVLWDETLPGGAHWSGLLRRGNTLRLIDVEGRA
                     NVSALFFNQEEKTERYNMPDTLKAQHTAHLTRGHALYSDMGRVLCSIPDDSCGWHDTV
                     CGVIDADTLRRRYGEASYQQRRNDMHRNGQDGLLVELGKWGMDRRDLVANVNFFSKAV
                     ADETGRLHFDTAHRAPGQHVDVRFEMNVLVILSAAPHPLDPATSYAPTPVHLTAWRSG
                     TAGADDVCRLHCPENGRGYINTERWFL"
BASE COUNT          921 a         1984 c         2047 g          936 t
ORIGIN      
        1 gccgaggcca gcaccgtcgc cgcatcggcg gccgtcagcg cgaacaccga gaccgcatcg
       61 agcgtgcgac aggccgggac gacgccggtg gtcgggatgc gccccaggct gggcttcagc
      121 ccgatcaggt tgttgaacgc agccggcacg cgacccgaac ctgccgtgtc ggtgccgagg
      181 gaaaagctcg cgagacccag tgccaccgaa acggcagacc cagacgacga gccgccggac
      241 acgaaggccg gatcgatcga gtttctgcag gccccgtagg gcgaacgcac gccgaccaga
      301 ccggtcgcga actggtcgag gttggtcttg cccaccggga tcgcacccgc gtcgatcagc
      361 cgctggacca ccgtcgcgct gcgttgcggc gtgtaggcga aggccggaca ggcggcggtg
      421 gtcggcacac cggcgaggtc gatgttgtcc ttgatggcga acggaatgcc gtacagcggc
      481 agcgtggccg gatcacggcc ttcgagcgcg ctggcatatc gcttcagcgc gtcgaggccg
      541 aggcgatgga tccatacgtt gcgcggcagg gccgcctcct cggcatcgat ccgggcgtgc
      601 agctcggtga cgagccgggt gggcgtcagc tcgccgctca ggtagcgcgt gcgcagcgca
      661 ggcagggaca ggtcgggtcg catcgggcgg tccttcggta acggtgtcag cgcagcagga
      721 ccagcggttg tccggcggtg accggctgtc cctccgagca aagcacttcg tgcaccacgc
      781 cggcgcgcgg cgcgagcacg gtgatctcca tcttcatcga ctccacgacg agcaaggtgt
      841 cgccggcctg caccgcactg cccttggcgg cgagcaccga ccagacgcca cccgacactt
      901 cggaggtgat cacttcgcca tcgaacgcac tggccgcggc ggcgcgtgcg tcgagatcgg
      961 catcgacggt ctcttccacc gtctcggcga cgccggcggc ccgccagcgt tcgcgttcgg
     1021 cttcgaaggc ggcctgctgg cgggtcttga acgcgctgat cgctgcgccg ttctccacca
     1081 ggtatcgctg gtagtcggcc agcctgaacg tgccgtcctg gatgcgcagg ccggcgcggc
     1141 ccgcgacgaa atccgagcgc agtttcagca actcgtcggc gctgaccgga tagaaccgca
     1201 actggtcgaa gaaccgcagc agccagggct tgccgggctc gaactccgaa gtgctcttcc
     1261 agcggttcca catctgcacc gtgcggccga cgaactggta gccgcccggg ccttccatgc
     1321 cgtagacgca caggtaggca cctccgatac cgaccgcgtt ctccggggtc caggtgcggg
     1381 ccgggttgta cttggtggtg acgagccggt gccgcggatc gaggggggtg gccaccggcg
     1441 cgccgaggta gacgtcgccc aggccgagca ccatgtagct cgcctcgaac accgtgtcgt
     1501 acacggcctg gatatcgtcc agcccgttga tgcgtcgtat gaactcgatg ttgctcggac
     1561 accagggcgc gtccgcacgc accgactgca tgtacttctc gatcgccagc cgcgtcgccg
     1621 cgtcgtccca ggacagcggc agccacacgg tgcggctcgg cacctcgatg tcggcggtcg
     1681 ccggcacgcc gtcctgtgcg tcgagcagcg cgtccatcag ggtggccagc gcgatgcgcg
     1741 acgggtcgta gtgcacctgc agcgagcgga tgcccggtgt caggtcgatg accccggcca
     1801 gcctgccctc gtcgcgccac gactcgagca actgcatcag cgcgtgcacg tgaaagcgca
     1861 gggtcaggtc gagcttgagc tcgccgaact cgatcagcac gtaggcatcg cccgcgcggc
     1921 gatagacgat ctcgatgccg tcgtccctgg cggcccggtg atgcagcacc gcggcatcgg
     1981 acagcaacgc gcgagaaatg ggcgcagcgg cgggcgcggt gttcgcaagg ggggcggttg
     2041 ccgacagcac cgcgtcctgc gcggcggcca gcgcacgtgc ctcggccagc gtcagcggct
     2101 tgaagcgcac ggtgttgccc ggcgacagct ggccgagctt ccacagctct gcctgcacca
     2161 cggtggccgg gcagacgaac ccaccgaggc tcgggccgtc cgggccgagg atcaccggca
     2221 tgtcgccggt gaagtcgatc gcgccgatcg cgtacgcgtt gtcgtggatg ttcgacgggt
     2281 gcaacccggc ctcgccgccg tcgctgcgcg cccaggtcgg cttcgggccg atcagcctga
     2341 cgccggtgcg gctcgagttg tagtgcacct gccagtccgt cgcgaagaag gtggcgacgt
     2401 cgtcgggcgt gaagaagtcg ggcgcggcat gcgggccgat cagcacgccg atgtcccagt
     2461 gggtgccgca ggccggcgtc agcatcgccg cctgttcggc gctgatcggc gccggggttg
     2521 ccgatactgg ttgtcccaga tgcagcacgt cgccgccgcg cagcacgcgc ccgccgtgcc
     2581 cgccgaactg gccgagcgtg aaggtcgatc cgctgcccag atattcgggc acgtcgatgc
     2641 cgcccgccac cgccaggtag gcacgacagc ccgccgcacc gctctggccg acgcgaccga
     2701 tgcgcaggtt ctgaccggcc tcgaccgtca ccgcgcgacc catcgggatg gcctttccat
     2761 ccagggacaa ctcgatcggc gcgccgcaga ccgcgacgac ggccgggcac ctgaagcgca
     2821 gggaggggcc ggcgacggtg atctccaggc cggcggcgcc ttgctcattg ccgagcagcc
     2881 ggttcgccag ccgcaacgcc agcggatcca tcggcccgga cggtggcacg ccgacatccc
     2941 agtagccgag tcgaccgggc cagtcctgca ccgaggtctg cacgccgggg ctgagcacgt
     3001 cgaaggcgcg actcggggcg ctgaagctgg ccaggctgcg ggtcgtgtgc atgccgtccg
     3061 cgaacggcgc aaacgacatc acggcgcgca ggtactcgag gttcgattcc agcccgtcca
     3121 catgcgtgac ggccagcccc ttcgacagct tcgcgatcgc gtctgcacgg tcgctgccgt
     3181 gcgcgatcag cttggccagc atcgggtcgt agtaggcggt gacttcggtg ccgcgttcga
     3241 tccaggtgtc gacccgcagg tcagcgggaa actccacccg agtcaacacg ccggtggccg
     3301 gctggaagtt cttgaagggg tcttcggcat acaggcgcgc ctgcaccgca tggccggttt
     3361 gagtgacgcg caggctgtcg agcgcaggca tctcgcctgc cccgagccgg accatccatt
     3421 cgacgatgtc gatgccggtg accgcctcgg tcacgccgtg ctcgacctgc agtcgcgcat
     3481 tcacctcgag gaagtagaag tcctgcgtgc tcgcatcgac gacgaactcg accgtgccag
     3541 ccgatcggta atgggctgcc tgagccagtc ggcgggccgt ggcgtgcagt gcggcgcgca
     3601 cctccggcct caggtgcggc gccggggctt cctcgacgac cttctggttg cgtcgctgca
     3661 gcgaacagtc ccgatcgccc agcgcaagca cctgccccgc gccgtcgccg aacacctgca
     3721 cctcgatgtg tcgcgcgtcg gtgatgtagc gctcgatgaa gacgccggca tccgagaaat
     3781 tgctctgcgc aaggcgcctc acggtatcga aggccgagcg cagctcatct gcgcctgcgc
     3841 acatctgcat gccgatgccg ccgcccccgg cgctgctctt gagcatcacc gggtagccga
     3901 tctgctcggc gcgggccagc gcctcgccca gatcggacag caggccggtt ccgggcagca
     3961 gcggaacacc actgtctgtg gccagcgcgc gcgcggtgtg cttcaggccg aacaggcgca
     4021 tctgctgcgg cgcgggcccg aggaagacca ggccggccgc ctcgcaggcc tcgacgaacc
     4081 cggcgttctc ggacaggaaa ccgtagccag ggtggatcgc cttcgctccg gtggccaggg
     4141 ctgcctcgat gatgcgtgca ccgtcgaggt aactcgacgc caccggcgcc gggccgatgc
     4201 acacggactc gtcggccatg gagacgtgca gtgcgcccgc atcggcttcg gaatagaccg
     4261 cgaccgagcc cacgccgagg cggcgcagcg tgcggatgat gcggcaggca atggcgccgc
     4321 ggttggctat cagtaccttg tcgaacatgg tggctcagac ggaatgcagg tcgtcctgcg
     4381 ttctgcgaat gcatgcgccg gtcgtccggc gatgcttggc tttcaggccg ggtcccacac
     4441 caggaagcgc acgggagtcg ggttgtagcc gttgcatggg ttgttcagct gcgggcagtt
     4501 cgacacgagc acgaccacat ccatctcggc gcgcatctcc acgtacttgc ccggcgcgga
     4561 gatgccgtcc tcgaaggtca gtttcccctc gggcgtgacc ggcacgttca tgaagaagtt
     4621 gatgttcgag accacgtcgc gcttggtcag tcgtgggatg cctgcggtgg attcgctgcc
     4681 cgaatcgatc cgggccagcg cgagcatgaa gctgtcgcgg cagctgtgca tgtagcgctt
     4741 ctcgagcgca tagcgaaccg tgttgctctc gcaggcgcag gctccgccca gcgtgtcgtg
     4801 gcggccgcag gtgtcggccg tgacggtgag catgggcctc gcctcgctcg acatcagcac
     4861 cgagcccacg gtcaggtaca gcgccccctg ggcctggatc gtgtgggtca ggctgtatcg
     4921 ctcgccgacg tcggcggcgc tgtagaagat cgtgtcgacg gcctggttgc cctcgatatc
     4981 gacgatgcgc aaggtctggc ccttcttcac caccttcgcc caggggtcgc cggcgaggca
     5041 gatctcgtcg atcaccgcat cggcagggag cagcgggctt tccacggtgg cggccatcac
     5101 aggaaccacc tttcggtgtt gatgtagccc cgtccgttct cggggcaatg gagacggcag
     5161 acatcgtcgg cacccgcggt gcctgagcgc caggcggtca ggtgcacggg tgtcggcgca
     5221 taggatgtcg cgggatcaag cgggtgaggc gcggccgaca ggatgaccag cacgttcatc
     5281 tcgaagcgca cgtcgacgtg ctgtccgggc gcgcggtgcg cggtgtcgaa gtgcagacgg
     5341 ccggtctcgt cggcgacggc cttgctgaag aaattgacgt tggcgaccag gtcgcgccgg
     5401 tccattcccc atttgcccag ttcgaccagc aagccgtcct gaccgttgcg gtgcatgtcg
     5461 ttgcggcgtt gctggtagct ggcctctccg tagcgccgac gcagggtgtc cgcatcgatc
     5521 acgccgcaga cggtgtcgtg ccagccgcaa ctgtcgtcgg gaatcgagca cagcacccgg
     5581 cccatgtccg aatacagcgc atgtccgcgc gtgagatgcg cggtgtgctg cgccttgagc
     5641 gtatcgggca tgttgtagcg ctcggtcttt tcttcctggt tgaagaacag cgccgagacg
     5701 ttggcgcggc cctcgacatc gatcagtcgc agcgtgttgc cgcgccgcag caggcccgac
     5761 cagtgcgcgc cgccgggcag ggtctcgtcc cacagaacga ggtcctgcgc gtagggcgcc
     5821 gatcgatccg gcacggccgc tcgggccagt ggggacagcg aattgaaatg ggtttgcatg
     5881 ggtcgtca
//
LOCUS       DAOOCX010000135         5883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_12765_length_5883_cov_8.647392, whole genome shotgun sequence.
ACCESSION   DAOOCX010000135 DAOOCX010000000
VERSION     DAOOCX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12765_length_5883_cov_8.647392"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PK399_10505"
     CDS             <1..510
                     /locus_tag="PK399_10505"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPW13590.1"
                     /translation="FLQSTRFDRTSTLGRRGLVSLAALDTAHYGHGRIDWWKFAPQLQ
                     RDGWLAPDDARTLALRGWFGALIGNSDMHLGNASLFLGDSRPLALAPSYDMLPMMLRP
                     ASTGEVVPRTLELVPPLPEQHALWSEAARMAQDFWWRVEVHAGISADMSRIAGEQARK
                     LQTAISRFG"
     gene            complement(512..1342)
                     /locus_tag="PK399_10510"
     CDS             complement(512..1342)
                     /locus_tag="PK399_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HPW13591.1"
                     /translation="MRKFRLTASACAAALLAGCAGPATRPDAVVARPVVPAATPTVAE
                     APVAMPMPPTMLDRIRRLAPALDPGVLALALEARACALRNGEVDDDAKLAVIDYSRPS
                     TEKRLWVFDVARAALLFNEHVAHGSGTGENIATRFSNDDGSHATSLGLFRTAETYQGG
                     NGYSLRMDGLDPGFNDHARSRAIVMHGAWYVNPDLIRTQGRLGRSQGCPALREQVAKV
                     VIDTLKQRQLLFAYANDAAFLQRGRSFACDGRSAGQILAAARAGTLGAGAAVGAVAAP
                     "
     gene            complement(1535..3430)
                     /locus_tag="PK399_10515"
     CDS             complement(1535..3430)
                     /locus_tag="PK399_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13592.1"
                     /translation="MNRRLLPVALSLLLAGGVHAQDASSVPPPRDVAYAPGTIRLEVD
                     ATNLNQRIFKVRQTIPVQAGALTLLYPAWVPGGHSPRNSIDKIAGLVFKAGDRKLVWK
                     RDPLNVYAFHLDVPQGVSQITAEFDFLSATGSGQGRVVMTPRMLNLQFLTTVLYPAGH
                     YAGRIMFDPRVTYPSGWTAFTALHEQGRSGDTVDYEDVPLDILVDSPVYAGRHAKNID
                     LTPAGSKVPVRLGVVADAAKFLEAKPEQIAIHKAMVAQAVKLFGAQHYDHYQFLFSLS
                     GQMGGNGLEHQRSSENGVGTDYFTGWNEKAGFTDLLAHEYTHSWNGKYRRGADLWTPN
                     YNVPMQDSLLWVYEGQTQFWGNVLAARSGMRPLAASRDALALVAATYADNRAGLQWRG
                     IQDTTNDPIVVGRASRAYLNYQMSEDYYRGGQMIWWEADALIRSKSGGRKSLDDFARA
                     FFGVNDGEWKVQNTYTFEDVVKTLDGVQPYDWTTFLRDRLDGKVALTGGIEASGWRLV
                     YKDEPNAHAKASSRGGADYTYSLGLSLNKDGVIGDVRWDSPAFKAGIGSGSTVVAVNG
                     LAYDKDVLDEAVTAAKTGAPIELMLRDFDVYRTIKLDYRGGLRYPHLERIEGKPDTLT
                     PMLTARR"
     gene            3565..3861
                     /locus_tag="PK399_10520"
     CDS             3565..3861
                     /locus_tag="PK399_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="I78 family peptidase inhibitor"
                     /protein_id="HPW13593.1"
                     /translation="MSLRPLSVLPAVFALAACASQPAADATTASPGLCNADAAQSHVG
                     KPATDANIQSAFKASGAKTVRSIKPGQAVTMDYREDRVNVYQDASGNIERISCG"
     gene            complement(3877..>4122)
                     /locus_tag="PK399_10525"
     CDS             complement(3877..>4122)
                     /locus_tag="PK399_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13594.1"
                     /translation="PADAQGRIQGSMSSLVSLAGIFAPAVFAGSFGFFIGPLAPVRLP
                     GIAFLIASLLLATAAFVAWRFTDAAHLQARTAGEPAA"
     assembly_gap    4123..4222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4223..4997)
                     /locus_tag="PK399_10530"
     CDS             complement(<4223..4997)
                     /locus_tag="PK399_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW13595.1"
                     /translation="MNAPLATGARKAALIFIFITVLIDVLAFGVIIPVLPHLVQEFVG
                     GDTSTAAYWTGAFAFAFSLVQFFSAPIQGALSDRYGRRPVILVSCLGLGLDFVFMALA
                     PNLAWLFVGRIISAATSASFTTANAYIADVTAPEERAKSFGMIGAAFGLGFIVGPLVG
                     GVLGDIDHRLPFWFAAGLAILNFCYGLFVLPESLPKEKRTPRFDWIHAKPMGGVKMLR
                     EYPQIWGLVAVVFIANFAHYVYPSTFVLFADASYGWKEKQ"
     gene            complement(5076..5666)
                     /locus_tag="PK399_10535"
     CDS             complement(5076..5666)
                     /locus_tag="PK399_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HPW13596.1"
                     /translation="MSSPSLRCTAFSGPQRIASGELRHVAMKAKQAFDLHPDRPVLVF
                     DDADARTIEVPLELPAADLLRLLAQPQLSAGTEAAPAPRKPGRPKLGVVAREITLLPR
                     HWDWLAAQPGGASVALRRLVEDARKVSAGEDRRRAAQEATYRFMQAMAGDAPGFEDAS
                     RALFAGDVTRFEEQVARWADDVRDHALWLATDAFAG"
     gene            complement(5706..5870)
                     /locus_tag="PK399_10540"
     CDS             complement(5706..5870)
                     /locus_tag="PK399_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13597.1"
                     /translation="MDARMQTSVDGIYAVGDVVSALNQISVAMGHAAIAASALHRRLP
                     PNWRERQVDA"
BASE COUNT          940 a         1950 c         1999 g          894 t
ORIGIN      
        1 ttcctgcaat cgacgcgctt cgaccgcacg tcgacgctgg gtcggcgcgg gctggtgtcg
       61 ctggccgcgc tggataccgc acattacggc cacggccgca tcgactggtg gaagttcgcg
      121 ccgcaactgc agcgcgacgg ctggctggct ccggacgatg cgcggacact cgccttgcgc
      181 ggttggttcg gtgcattgat cggcaacagc gacatgcacc tgggcaatgc cagcctgttc
      241 ctcggcgaca gccgcccgct ggcactggca cccagctacg acatgctgcc gatgatgctg
      301 cggccagcgt ccaccggtga agtggtgcca cgcacgctcg aacttgttcc gccgttgccc
      361 gaacaacacg cgctctggag cgaggccgca cggatggcgc aggacttctg gtggcgcgtg
      421 gaagtccacg cagggatttc cgccgacatg tcccggatcg cgggcgaaca ggcgcgcaag
      481 ctccagaccg caatctcgcg gttcggttga atcagggcgc cgccaccgca ccgacggcag
      541 caccggcgcc cagcgtgcca gcgcgcgcgg cagcgaggat ctgcccggca ctgcggccat
      601 cgcaggcaaa gctgcggcca cgttgcagga acgccgcatc attggcatag gcgaacagca
      661 gctggcgctg cttcagcgtg tcgatgacga ccttggccac ctgctcgcgg agcgccgggc
      721 aaccctggct gcggccaaga cgaccctggg tgcggatcag gtcggggttc acgtaccagg
      781 cgccgtgcat gacgatcgca cgcgagcggg cgtggtcgtt gaagccggga tccagtccgt
      841 ccatgcgcag cgaatagccg ttgccgccct ggtaggtttc ggcggtgcgg aacaggccca
      901 ggctggtggc gtggctgccg tcatcgttgg agaagcgcgt cgcgatgttt tcgccggtac
      961 cggagccgtg ggccacgtgt tcgttgaaca gcagcgcagc gcgggccacg tcgaagaccc
     1021 acaggcgctt ctcggtggac gggcgcgagt agtcgatcac cgcgagcttc gcatcgtcat
     1081 cgacctcgcc gttgcgcagg gcgcaggcgc gcgcttccag ggcgagggcg agcacgccgg
     1141 gatcgagcgc gggcgcgagg cggcggatgc gatccagcat cgtgggcggc atcggcatgg
     1201 cgacgggcgc ttcggcaacg gtgggcgtcg ccgccggcac caccggacgc gccaccaccg
     1261 cgtcggggcg tgttgccggg cccgcgcagc cggccagcag ggcggccgcg cacgcggagg
     1321 cggtcagtcg gaacttgcgc atgccgcgac cttacctttc atcaccgctt gatgtcgctg
     1381 aatgcggcgc aagaacctga acactaacaa taacttaacg cgactttgtg acgaattgcg
     1441 gggaatgcgg cggcgcggct atcaccacca ctcgccgcgc cgggttggcg cggcgtgccg
     1501 cccagcggtt gcccggcggg ggtgggggcg cgactcaacg ccgcgcggtc agcattggcg
     1561 tgagggtgtc cggcttgcct tcgatgcgct ccaggtgcgg atagcgcagg ccgccgcggt
     1621 agtcgagctt gatcgtgcgg tagacgtcga agtcacgcag catgagttcg atcggcgcgc
     1681 cggtcttcgc cgcggtgacc gcttcgtcca acacatcctt gtcgtaggcc aggccgttga
     1741 ccgcgaccac ggtactgccg ctgccgatgc cggccttgaa cgccgggctg tcccagcgca
     1801 cgtcgccgat cacgccgtcc ttgttcaacg acaggcccag cgagtaggtg tagtcggcgc
     1861 cgccgcgcga actggccttg gcatgggcgt tcggctcgtc cttgtagacc agcctccagc
     1921 cgctggcctc gatgccgccg gtgagggcga ccttgccgtc caggcgatcg cgcaggaagg
     1981 tcgtccagtc gtagggctgc acgccgtcga gggtcttcac cacgtcctcg aaggtgtagg
     2041 tgttctgcac cttccattcg ccgtcgttga cgccgaagaa cgcgcgggcg aagtcgtcca
     2101 gcgacttgcg gccgccgctc ttgctgcgga tcagcgcatc ggcttcccac cagatcatct
     2161 gcccgccgcg atagtagtcc tcgctcatct ggtagttcag gtacgcacgc gacgcgcggc
     2221 cgaccacgat cggatcgttg gtggtgtcct ggataccgcg ccactgcagg ccggcgcgat
     2281 tgtccgcata cgtcgccgcg accagcgcca gcgcatcacg cgaggctgcg agcggacgca
     2341 tgccgctgcg cgcggcaagg acgttgcccc agaactgggt ctgcccctcg tacacccaca
     2401 gcaggctgtc ctgcatcggg acgttgtagt tcggcgtcca caggtcggcg ccgcggcggt
     2461 acttgccgtt ccaggagtgg gtgtattcgt gcgccagcag gtcggtgaag ccggccttct
     2521 cgttccagcc ggtgaagtag tcggtgccga cgccattctc gctggagcgc tggtgctcca
     2581 ggccgttgcc gcccatctgc ccggacagcg agaacaggaa ctggtagtgg tcgtagtgct
     2641 gcgcgccgaa cagcttcacc gcctgcgcca ccatcgcctt gtggatggcg atctgttccg
     2701 gcttggcctc taggaatttc gcggcatcgg cgaccacgcc caggcgcacc ggcaccttgc
     2761 tgccggccgg ggtcaggtcg atgttcttcg catggcggcc ggcatacacc ggcgagtcca
     2821 ccaggatgtc gagcggcacg tcctcgtaat cgaccgtgtc gccgctgcga ccctgctcgt
     2881 gcagcgcggt gaaggcggtc cagccgctgg gataggtcac ccgcggatcg aacatgatcc
     2941 tgccggcgta atgccccgcg gggtacagca cggtggtgag gaactgcagg ttgagcatgc
     3001 gcggggtcat caccacccga ccctggccgc tgccggtcgc gctgaggaaa tcgaactccg
     3061 cggtgatctg ggatacgccc tgcggcacgt cgaggtggaa ggcgtagaca ttcagcgggt
     3121 cgcgcttcca taccagcttg cggtcgccgg ccttgaacac cagcccggcg atcttgtcga
     3181 tcgaattgcg cggcgagtgc ccacccggta cccacgccgg atacaacagc gtcaatgcgc
     3241 cggcctgcac cgggatggtc tgcctgacct tgaagatgcg ctggttgagg ttggtggcgt
     3301 cgacttccag ccggatcgtg ccgggcgcgt aggccacgtc gcgcggcggc gggaccgatg
     3361 atgcgtcctg cgcgtggacg ccgccggcaa gcaacaggga cagggcgacg ggcaacaggc
     3421 gacgattcat cggcaactcc aggcggggaa cgtgacccac catcgtaggc aacacgcctg
     3481 tcgtcgcgcc tgccagaagc catgcacgcg ggcttaatcc gcgcccgacc atgctcactc
     3541 catcacagtc ccgggaatcc gcccatgtcg ctgcgcccct tgtccgtgct tcccgcggta
     3601 ttcgcgctcg cggcctgcgc ctcgcaaccc gccgccgacg ccacgaccgc cagccccggc
     3661 ctctgcaatg ccgacgccgc gcagtcccat gtcggcaagc ccgccaccga cgccaacatc
     3721 cagtccgcgt tcaaggcgtc tggcgcgaag acggtgcgct cgatcaagcc cggccaggcg
     3781 gtgacgatgg attaccgcga ggatcgggtg aacgtgtacc aagacgccag cggcaacatc
     3841 gaacggatca gctgcggctg atcgcgtgca tcgtcctcag gccgcgggtt cgcctgcggt
     3901 cctggcctgc aggtgcgcgg catcggtgaa gcgccacgcc acgaaggcgg cggtcgccag
     3961 cagcagcgac gcgatcagga aggcgatgcc gggcaggcgc accggcgcca gcgggccgat
     4021 gaagaacccg aacgaaccgg cgaacaccgc cggcgcgaag atcccggcca gcgacaccag
     4081 gctgctcatc gagccctgga tgcggccctg cgcatcggcc ggnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nncctgcttt tccttccagc cgtagctggc gtcggcgaac
     4261 agcacgaagg tgctcgggta gacgtagtgg gcgaagttgg cgatgaacac caccgccacc
     4321 aggccccaga tctgcgggta ctcgcgcagc atcttcacgc cacccatcgg cttggcatgg
     4381 atccagtcga aacgcggcgt gcgcttctcc ttcggcagcg attccggcag cacgaacagg
     4441 ccgtagcaga agttgaggat cgccagtccc gcggcgaacc agaacggcag gcgatggtcg
     4501 atgtcgccga gcacgccgcc gaccagcggg ccgacgatga agcccaggcc gaacgccgcg
     4561 ccgatcatgc cgaagctctt cgcgcgttct tccggcgcag tgacgtcggc gatgtaggca
     4621 ttcgcagtcg tgaagctggc cgaggtcgcc gccgagatga tccggccgac gaacagccac
     4681 gccaggttgg gcgccagcgc catgaacacg aagtccaggc cgagccccag gcaggagacc
     4741 aggatcaccg ggcgccggcc atagcgatcg gagagagcgc cctggatcgg cgcactgaag
     4801 aactgcacca gcgagaacgc gaacgcgaac gcaccggtcc agtacgccgc ggtggaggtg
     4861 tcgccaccga cgaactcctg caccaggtgc ggcagcaccg ggatgatcac cccgaacgcc
     4921 agcacgtcga tcagcacggt gatgaagatg aagatcagcg cggccttgcg tgcgccggtg
     4981 gcgaggggtg cgttcaaggg gtgtcccgat gtgccgtgcg gtttgtctgc atcgccgacc
     5041 gttgccggga tgaccaatgg tgaggcgcgc gattgtcagc cggcgaaggc gtccgtggcc
     5101 agccacagtg catggtcgcg gacatcgtcg gcccagcgcg cgacctgctc ctcgaagcgg
     5161 gtcacgtcgc cggcgaacag cgcgcgcgat gcgtcctcga aaccgggtgc atcgcccgcc
     5221 atcgcctgca tgaagcggta cgtcgcttcc tgcgcggcgc ggcggcggtc ctcgccggcg
     5281 gagaccttgc gcgcgtcttc caccagccgg cgcagggcga ccgaggcccc gcccggctgc
     5341 gcggccagcc agtcccagtg ccgcggcagc aaggtgatct cgcgggcgac cacgcccagc
     5401 ttgggccggc cgggtttgcg cggggccggc gctgcctcgg tgccggccga aagctgcggt
     5461 tgcgcgagca ggcgcagcag gtcggcggcg ggcagctcca gcgggacttc gatggtgcgg
     5521 gcgtcggcat cgtcgaacac cagcaccggc cggtccggat gcaggtcgaa ggcctgcttg
     5581 gccttcatcg cgacatggcg gagttcgccg ctggcgatcc gttgcgggcc ggaaaaggcc
     5641 gtgcaacgca gggatggact ggacatgggg cctccggact gagactgtat tttacccgga
     5701 caaaatcagg cgtcaacctg ccgttcgcgc cagttgggcg gcaaccgccg gtgcagcgcg
     5761 gaggcggcga tcgccgcatg gcccattgcc acgctgatct ggttgagcgc gctgaccacg
     5821 tcgccgacgg cgtagatccc gtccacgctg gtctgcatgc gcgcatccac gatgagctcg
     5881 ccg
//
LOCUS       DAOOCX010000136         5855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_12854_length_5855_cov_8.883966, whole genome shotgun sequence.
ACCESSION   DAOOCX010000136 DAOOCX010000000
VERSION     DAOOCX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12854_length_5855_cov_8.883966"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /gene="typA"
                     /locus_tag="PK399_10545"
     CDS             <1..1601
                     /gene="typA"
                     /locus_tag="PK399_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPW13598.1"
                     /translation="GHADFGGEVERVLSMVDSVLILVDAMDGPMPQTRFVTQKAFAMG
                     FKPIVVVNKIDRPGARPDWVIDQVFDLFDKLGATNEQLDFPIVYASALHGYASLDDSA
                     REGDMTPLYEAIMQHVAPPQVDMDGPFQMRISQLDYSNFVGLIGIGRIQRGKVRRNMP
                     VSVVDREGKKRQGKILQVLGFMGLERIETEEAGAGDIVAISGVADLSISDTVCALDAP
                     EALPALTVDEPTISMTFQVNNSPFAGNKDMSGGKFLTSRQLRERLERETLHNVALKVE
                     EGSDPDKFLVSGRGELHLSVLIENMRREGFELAVSRPEVIIKEIDGQLMEPVEQLVVD
                     IEEVHQGGVMEKLGTRKGALKNMESDGKGRVRLDYMIPARGLIGFQNEFRTLTQGSGL
                     LFHVFDHYGPKETGAIAKRQNGVMIANAAGTTPAYSLGPLQERGKLFAAEGDHVYEGQ
                     LVGIHSKDNDLTVNAIKPKPLTNMRASGKDDAIQLSPAIKYTLEQALDFIEDDELVEV
                     TPKEIRLRKKFLTESDRKRASRSA"
     gene            1625..2170
                     /locus_tag="PK399_10550"
     CDS             1625..2170
                     /locus_tag="PK399_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HPW13599.1"
                     /translation="MSVFLGWMPDAATQAALADLHHRIRAALPADAPRHDWRTPAQWH
                     VTLRYLGESIDDAQRTRIDAAMIELAATTHAPDASILGAQYWPHAKVLVAKIEASDAL
                     KRLLKQLEATMQDCGFAKERAQTAHVTLAYLAHRDAPAQPTPLATPVPLRIDRLQLLR
                     TVPGAYMSLASWPLASIATDA"
     gene            2167..2634
                     /locus_tag="PK399_10555"
     CDS             2167..2634
                     /locus_tag="PK399_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HPW13600.1"
                     /translation="MTQQDYNPDPHAHPGLHVIAVVEAIKGALALLAASGLELLGPAP
                     LQRWLAELIAHFQLDPDTGAMAWLAHAINPGAVHLAAAVAALYGIVHLVEGWGLWRAK
                     AWASWLGCITAALYLPFDIYAFATHRHWLEGVVVVVNLLVVWVLARDLRKRTR"
     assembly_gap    2793..2892
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2893..4047
                     /locus_tag="PK399_10560"
     CDS             <2893..4047
                     /locus_tag="PK399_10560"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPW13601.1"
                     /translation="AGAVILGKTNLSEWANFRSTRSTSGWSGRGGQTHNPYALDRNPC
                     GSSAGTGSAIAANLAAVGIGTETDGSILCPSAVAGLVGLKPTVGLVSRSGIIPISASQ
                     DTAGPMTRSVADAAALLTAIAAGDDTDAATREYAARTAIDYTTHLKVDALKGARIGVV
                     RKLMGYQPDTDAAFERAIAAMKAAGATVVDAEIPTLGQWDKDEYEVLLYEFKDGLNRY
                     LASSGAPHKTLADLIAFNTANAATEMPFFAQEIFEQANAKGPLTEPAYLAARDKARRL
                     AGPQGIDAALKAQQLDALVAPAMSPAWPTDPVNADHFTGAGYGAAAVARYPSITVPMG
                     DVHGLPVGIAFMGTAWSEGRLIELAYAYEQASKARKPPRFPATITVGAAE"
     gene            complement(4061..5455)
                     /locus_tag="PK399_10565"
     CDS             complement(4061..5455)
                     /locus_tag="PK399_10565"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HPW13602.1"
                     /translation="MSSVPAVSLAPPPPAANDPWAPGSWRAKPALQLPQYPDATALAA
                     AQDELRALPPLVTSWEILTLKQQLAEAQEGKRFLLQGGDCAETFAECNAEIISNRLKV
                     LLQMSLVLVHGLKLPVVRVGRFAGQYAKPRSADLETKGEETLPSYRGDMVNGPEFTPE
                     ARIPDPRRMIKAHARSAMTMNFVRSLIDGGFADLHHPEYWGLGWVSQSPHAVEYQKMV
                     AGIGDAVRFMETLAGGQMHNLNRVDFYTSHEALLLPYEEAQTRQVPRQPGWFNLSTHF
                     PWIGMRTAALDGAHTEYFRGIRNPIAVKVGPSVTPDQLLRLVDVLNPDNEPGRLGLIH
                     RLGAEHIAAKLPTLLDAVKREGRRVLWICDAMHGNTESTSNGYKTRRFANIRSEIEQA
                     FDLHAAAGTKLGGVHLELTGENVTECLGGARDLTEIDLQRAYRTTVDPRLNYEQALEI
                     AMLIVRKRGPIASA"
     gene            complement(5515..>5855)
                     /locus_tag="PK399_10570"
     CDS             complement(5515..>5855)
                     /locus_tag="PK399_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HPW13603.1"
                     /translation="HSPRAPGGRKAWSLLAFVAAAVGAGYSGRHSWVQLNPPAMPSCG
                     PGLNFIVEQHSWLGAARKVLLATGDCSAIDWKFLGLSMPMWCLVFFVALGLGALYAGF
                     RRRHHHRFHR"
BASE COUNT          995 a         1989 c         1911 g          860 t
ORIGIN      
        1 cgggccacgc cgacttcggc ggcgaggtcg agcgcgtgct gtcgatggtc gactccgtgt
       61 tgatcctggt cgacgcgatg gacggcccga tgccgcagac ccgcttcgtg acccagaagg
      121 cgttcgcgat gggcttcaag ccgatcgtcg tcgtcaacaa gatcgaccgc cccggtgcgc
      181 ggcccgactg ggtgatcgac caggtgttcg acctgttcga caagctcggc gcgaccaacg
      241 agcagctgga tttcccgatc gtctacgcgt cggcgctgca cggctacgcc agcctcgacg
      301 acagcgcccg cgagggcgac atgaccccac tgtacgaagc gatcatgcag cacgtggcgc
      361 cgccgcaggt cgacatggac ggcccgttcc agatgcgcat cagccagctg gactacagca
      421 acttcgtcgg cctgatcggc atcggccgca tccagcgcgg caaggtccgc cgcaacatgc
      481 cggtcagcgt ggtcgaccgc gaaggcaaga agcgccaggg caagatcctg caggtgctgg
      541 gcttcatggg cctggagcgc atcgaaaccg aggaagcagg cgcgggcgac atcgtcgcca
      601 tctccggcgt cgccgacctg tcgatctccg acaccgtgtg cgcgctcgac gcgccggaag
      661 cgctgccggc gctgaccgtg gacgagccga ccatcagcat gaccttccag gtgaacaatt
      721 cgccgttcgc cggcaacaag gacatgagcg gcggcaagtt cctgaccagc cgccagctgc
      781 gcgagcgcct ggagcgcgag accctgcaca acgtggcgct gaaagtcgag gaaggctcgg
      841 atccggacaa gttcctggtc tcgggccgcg gcgagctgca cctctcggtg ctgatcgaga
      901 acatgcgccg cgaaggcttc gagctggcgg tatcgcgccc ggaagtgatc atcaaggaaa
      961 tcgacggcca gctgatggag ccggtcgagc aactggtggt cgacatcgag gaagtgcacc
     1021 agggcggcgt gatggagaag ctcggcaccc gcaagggcgc gctgaagaac atggaatccg
     1081 acggcaaggg ccgcgtgcgc ctggactaca tgatcccggc gcgtggcctg atcggcttcc
     1141 agaacgagtt ccgcaccctg acccagggct cgggcctgct gttccacgtg ttcgaccatt
     1201 acggcccgaa ggaaaccggc gcgatcgcca agcgccagaa cggcgtgatg atcgccaatg
     1261 ccgccggcac caccccggcc tattcgctcg gtccgctgca ggaacgcggc aagctgttcg
     1321 ccgccgaagg cgaccatgtg tacgagggcc agctggtcgg catccactcg aaggacaacg
     1381 acctcacggt caacgccatc aagccgaagc cgctgaccaa catgcgcgcc tccggcaagg
     1441 acgacgcgat ccagctcagc ccggcgatca agtacaccct ggagcaggcg ctggacttca
     1501 tcgaggacga cgagctggtg gaggtcaccc cgaaggaaat ccgcctgcgc aagaagttcc
     1561 tgaccgaaag cgaccgcaag cgcgcttcgc gctcggcgta accggcacgc gcgaggaaca
     1621 cgcaatgagt gtgttcctgg gctggatgcc cgacgcggcc acgcaggccg cgttggccga
     1681 cctgcaccac cgcatccgcg cggcactgcc cgcggatgca ccgcgccatg attggcgcac
     1741 gcccgcgcag tggcacgtga ccctgcgcta tctcggcgaa tccatcgatg atgcgcaacg
     1801 cacgcgcatc gatgccgcga tgatcgaact cgcggcaacg acgcatgcgc ccgatgcctc
     1861 catcctcggc gcgcagtact ggccgcatgc gaaggtgctg gtcgcgaaga tcgaggcctc
     1921 ggacgcgctc aagcgcctgt tgaaacaact cgaagcgacg atgcaggact gcggcttcgc
     1981 caaggagcgc gcgcagaccg cgcacgtcac ccttgcctac ctggcccatc gcgatgcgcc
     2041 agcgcagccg acgccactgg ccaccccggt gccgttgcgc atcgatcgcc tgcagctctt
     2101 gcgcacggtg cccggcgcct acatgtcgct tgcatcgtgg ccgttggcca gcatcgcaac
     2161 ggacgcatga cccagcagga ttacaacccg gacccgcatg cgcatcccgg cctgcatgtc
     2221 attgccgtgg tggaggccat caagggggcg cttgccctgc tcgccgccag cgggctggaa
     2281 ttgctcggcc ccgcgccgct gcagcgctgg cttgcggaac tcatcgccca tttccagctc
     2341 gaccccgaca ccggcgcgat ggcgtggctg gcgcatgcga tcaacccggg ggccgtgcac
     2401 ctggcggcgg ccgtggcggc gctgtacggc atcgtccacc tggtcgaggg ctggggcctg
     2461 tggcgggcga aggcgtgggc gtcatggctg ggctgcatca ccgcggcgct gtacctgccg
     2521 ttcgacatct acgcattcgc cacccaccgg cactggcttg aaggcgtggt ggtcgtggtc
     2581 aacctgctgg tggtctgggt gctggcgcgc gacctgcgca agcgcacgcg ttgagttgat
     2641 ccccggcgcg gcaacgggga tgcttgcgca tcctcgaccg gagccacgcc gatgcgccgc
     2701 cttgcccttg cgacttcgtt gtcgctgctc gttgcctgcc aacccacggc gcaaaccccg
     2761 gccgccgccg atgcacccgc gcccgtcgcg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nngccggcgc ggtgatcctg ggcaagacca acctcagcga atgggccaac
     2941 ttccgctcca cccgctcgac ctccggctgg agcgggcgcg gtgggcagac ccacaatccc
     3001 tacgcgctcg accgcaatcc ctgcggatcc agcgccggca ccggcagcgc gatcgccgcg
     3061 aacctcgcag cggtcggcat cggcaccgaa accgacggca gcatcctctg cccctccgcg
     3121 gtagcgggcc tggtcggcct caagcccacc gtggggctgg tcagccgcag cggcatcatc
     3181 ccgatctcgg cctcgcagga caccgccggc ccgatgacgc gcagcgtggc cgatgccgcc
     3241 gccctgctga ccgcgattgc cgcgggcgac gacaccgatg cggcgacacg cgagtacgca
     3301 gcgcgcaccg ccatcgacta caccacccac ctcaaggtcg atgcgctgaa gggtgcgcgc
     3361 atcggcgtgg tccgcaaatt gatgggctac cagccggata ccgatgccgc gttcgagcgt
     3421 gcgatcgcgg cgatgaaggc cgccggcgcc accgtggtcg atgcggagat cccgaccttg
     3481 gggcaatggg acaaggacga gtacgaggtg ctgctgtacg aattcaagga tggcctcaat
     3541 cgctacctcg ccagcagcgg cgcgccgcac aagacgctgg ccgacctgat cgcattcaac
     3601 actgcgaatg ccgcgaccga gatgccgttc ttcgcgcagg agatcttcga gcaggcgaat
     3661 gcgaagggcc cgttgaccga gcccgcctac ctcgccgcgc gcgacaaggc gcgcaggctc
     3721 gccgggccgc agggcatcga cgccgcattg aaagcgcaac aactcgatgc gctggtcgca
     3781 ccggcgatgt cgcccgcctg gccgaccgac ccggtcaacg ccgaccattt caccggggcg
     3841 ggctacgggg cggccgcggt cgcgcgctat cccagcatca ccgtgccgat gggcgacgtg
     3901 catggcctgc cggtcggcat cgccttcatg ggcacggcgt ggagcgaagg ccgcttgatc
     3961 gaactcgcgt acgcctacga gcaggccagc aaggcgcgca agccaccgcg tttcccggcg
     4021 accatcacgg tcggcgcggc ggagtgacca gcggggtcga tcaggcgctg gcgatcggcc
     4081 cgcgcttgcg cacgatcagc atcgcgatct ccagcgcctg ttcgtagttg aggcgcggat
     4141 ccacggtggt gcggtaggcg cgctgcaggt cgatctcggt gaggtcgcgc gcgccgccca
     4201 ggcattcggt gacgttctcg ccggtcagtt ccaggtgcac gccgcccagc ttcgtgccgg
     4261 cggccgcgtg caggtcgaac gcctgttcga tctcgctgcg gatgttggcg aagcggcggg
     4321 tcttgtagcc gttgctggtc gattcggtgt tgccgtgcat ggcatcgcag atccacaaca
     4381 cgcgacgacc ctcgcgcttc accgcatcga gcagggtcgg cagcttggcc gcgatgtgct
     4441 ccgcacccaa gcggtggatc aggccgaggc ggcccggttc gttgtcggga ttcagcacgt
     4501 cgaccaggcg cagcaactgg tccggcgtca ccgacggccc gaccttcact gcgatcgggt
     4561 tgcggatccc tcggaagtat tcggtgtgcg cgccatccag cgcggcggtg cgcatgccga
     4621 tccacgggaa atgcgtgctc aggttgaacc agcccggctg gcgcggcacc tggcgcgttt
     4681 gcgcctcctc gtacggcagc agcagggctt cgtgcgaggt gtagaaatcg actcggttga
     4741 ggttgtgcat ctgcccgccg gccagggtct ccatgaagcg cacggcatcg ccgatgccgg
     4801 cgaccatctt ctggtactcg accgcgtgcg gcgactggct gacccagccc agcccccagt
     4861 attccggatg gtgcaggtcg gcgaagccgc catcaatcaa cgaacgcacg aagttcatcg
     4921 tcatcgcaga acgcgcatgc gccttgatca tccggcgcgg atccgggatg cgggcctcgg
     4981 gagtgaattc cggtccgttg accatgtcgc cgcggtagct cggcagggtc tcctcaccct
     5041 tggtttccag atcggccgaa cgcggcttgg cgtactgccc ggcgaagcga cccacgcgaa
     5101 ccaccggcaa cttcagcccg tgcaccagca ccaggctcat ctgcaacagc accttgaggc
     5161 ggttggagat gatctcggca ttgcactcgg cgaaggtctc ggcgcaatcg ccgccctgca
     5221 gcaggaagcg cttgccttcc tgcgcctccg ccaactgctg cttgagcgtc aggatttccc
     5281 acgacgtcac caacggcggc aacgcgcgca actcgtcctg cgcggcggca agcgccgtgg
     5341 catccggata ttgcggcagt tgcagcgctg gcttcgcgcg ccagctgccg ggcgcccacg
     5401 gatcgttggc ggcgggcggc ggcggggcga gcgaaacggc ggggacggac gacatgtcga
     5461 tgatcacggg atgcggcagt gcatcatccc gcccgacccg cgtccccgca accgttaccg
     5521 gtggaaccga tggtgatggc gacgtctgaa accggcatac agcgcaccca gcccgagcgc
     5581 gacaaagaac accaggcacc acatcggcat gctcaggccg aggaacttcc aatcgatcgc
     5641 gctgcagtcg ccggtggcga gcaggacctt gcgcgccgca cctaaccagg aatgctgctc
     5701 gacgatgaag ttcaggccgg gaccacagct gggcatcgcc ggtggattca gctgcaccca
     5761 cgaatgtcgg ccggagtagc cggcgccgac cgcggcggca acgaacgcaa gcagcgacca
     5821 ggccttgcgg ccaccgggcg cgcgcggcga atgca
//
LOCUS       DAOOCX010000137         5796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13046_length_5796_cov_6.951228, whole genome shotgun sequence.
ACCESSION   DAOOCX010000137 DAOOCX010000000
VERSION     DAOOCX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13046_length_5796_cov_6.951228"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PK399_10575"
     CDS             <1..405
                     /locus_tag="PK399_10575"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159046.1"
                     /note="E3 component of 2-oxoglutarate dehydrogenase
                     complex; catalyzes the oxidation of dihydrolipoamide to
                     lipoamide; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPW13604.1"
                     /translation="IAGLPGHVNFDTIPWVIYTEPEIAWVGKTEEQLKAEGIPYKAGS
                     FPFAAVGRAVAMAEPAGFVKVLAHAETDTILGMHLVGVNVSELVHEGVLAMEFKGSAD
                     DLARICHAHPSLSEAVHDAAMAVHKRAIHKAN"
     gene            475..1086
                     /locus_tag="PK399_10580"
     CDS             475..1086
                     /locus_tag="PK399_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPW13605.1"
                     /translation="MKTICVYCGSNAGNDPAYAAQAEALGARLAADGIALVYGGGNVG
                     LMGIVADAVLANGGEVIGVIPQQLVDWEVAHRGVTRLEVVDSMHTRKARMFELSDGFV
                     ALPGGFGTLDEMFEMLTWRQLGLGKKPCAFLDVNGFWQPLMAMLDTMVRERFLHAEQR
                     DDLWHGEDIDALLAWMRGYVPAQADKWLDEKRRSQLKLTPEDV"
     gene            1083..2087
                     /locus_tag="PK399_10585"
     CDS             1083..2087
                     /locus_tag="PK399_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HPW13606.1"
                     /translation="MSVPANFRAFRIHDDAQGYRSGIEAISLDDLNAGEVIVKGAYSS
                     VNFKDALAGTGQGKILRRFPLVGGIDVAGHVVASTDPAFKEGEAVLVTGSGLSETRDG
                     GYAEYARLESKWVVALPTGLSLRESMILGTAGFTAALALLRMEDNRQSPSLGPLAVTG
                     ATGGVGSLAIAIFSKAGYDVHAISGKAGQAGYLKSLGASEVLGRDALATTRPMDTARF
                     GGGLDNVGGPMLAALLAQTVPYGNVATAGLAASHELHATVMPFIIRGVSLLGVASAGT
                     ARDLRDEVWRRLSSDWKPAGLEAICTRETTLDGLPGVFATMLAGDSLGRTVVRLAESA
                     "
     gene            2143..2505
                     /locus_tag="PK399_10590"
     CDS             2143..2505
                     /locus_tag="PK399_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW13607.1"
                     /translation="MARILIVDDSPSQLMGIRRIVEKLGHDALTAEDGAAGVEAAKRE
                     IPDLILMDVVMPNLNGFQATRSITREPTTKHIPVILVTTKDQDTDRVWGMRQGAKAYI
                     TKPFSESELSELIVQYLA"
     gene            complement(2614..3525)
                     /locus_tag="PK399_10595"
     CDS             complement(2614..3525)
                     /locus_tag="PK399_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPW13608.1"
                     /translation="MEFKDYYGVLGVEPSAGDAEIKTAYRRLARKFHPDVSKEVGAED
                     KFKAVNEAYEALRDPEKRKAYDQLRAGGYRPGDQVRPPPGGFRGGPGGHPDFDEIFAG
                     GGAGGGFSDFFESMFGRGRAGAGARGPQAGGQPRDGTTRAKLAVPLESVYAGGNVRIG
                     VNGRTLEVRVPKGIKPGQSIRLARQGNNGGDLLLEIEYAAHPRFEVDGRNVIHVLPVA
                     PWEAALGATLSVPTLGGSVELKIPADSEAGRKLRLRGRGLPGGTQGADGDQIVELEIL
                     APRAHDDAQRAAYATMRDAFGDDWRRA"
     gene            3619..4101
                     /locus_tag="PK399_10600"
     CDS             3619..4101
                     /locus_tag="PK399_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPW13609.1"
                     /translation="MSIQPGERIPEAILQYVADGVHKIDTLTLFEGRKLVLFSVPGAF
                     TPTCSERHLPGFVEHFDAFRERGIEVACMAVNDPFVMQAWGESQHVPAGMRMLADGNA
                     DFTRALGLEMDASGFGMGTRSKRFALYAEDGVVRELFVEAPGEFRVSSAENVLAHLPG
                     "
     gene            complement(4123..>4308)
                     /locus_tag="PK399_10605"
     CDS             complement(4123..>4308)
                     /locus_tag="PK399_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13610.1"
                     /translation="LRALGGEGRVQWLLDGRWIGETDGGRSLVHVFDTPGAHELTALA
                     DSGAWHGIRFSMLAPAR"
     assembly_gap    4309..4408
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4409..>5796)
                     /gene="pbpC"
                     /locus_tag="PK399_10610"
     CDS             complement(<4409..>5796)
                     /gene="pbpC"
                     /locus_tag="PK399_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HPW13611.1"
                     /translation="GGSTLTMQVARILDPGLRGSRTRTPLGKLRQALRAVQLEAHLSK
                     HEILTLYLERAPFGGTIEGVEAASWAYLGKPAARLSHAEAALLAVLPQAPSRLRPDRS
                     PDAARQARDKLLARMASSGTWTRAQVDDARIEGVVSRRLRPPMDAALLAQRLRTTYPK
                     AERIASTLDASLQRTLEERVSTYFSDLPPRTSAALLVVDNATLEARAYVGSVAFGDKL
                     RLGHVDMVQAWRSPGSTLKPFLYGMALDAGMIHSESLLVDAPQAFGGYRPSNFDAAFN
                     GPVGAATALRLSLNVPAVDLLERVGPARFAARLEHAGIALKFPRGASPNLSMILGGTG
                     ARLEDLVGAFAAPHRDGIAGRVRYSPADPRIERRLLSPGAAWIVREVLAANPRPGESE
                     GTFDTGRRPRVAWKTGTSYGYRDAWAIGGTRRYTVGVWVGRPDGTPMPGQYGAVTALP
                     LLFEVVDSLPRQ"
BASE COUNT          898 a         2000 c         1938 g          860 t
ORIGIN      
        1 atcgccggcc tgcccggcca cgtcaatttc gacaccattc cgtgggtgat ctacaccgag
       61 ccggaaatcg cgtgggtcgg caagaccgag gagcagctga aggcggaagg catcccgtac
      121 aaggccggca gcttcccgtt cgccgcggtg ggccgcgcgg tggcgatggc cgagccggcg
      181 ggcttcgtga aggtgctggc gcatgccgag accgacacca tcctgggcat gcacctggtc
      241 ggcgtgaacg tctccgagct ggtccacgaa ggcgtgctgg cgatggagtt caagggcagt
      301 gccgacgacc tggcgcgtat ctgccacgcg catccatcac tgtccgaagc cgtgcacgat
      361 gcggcgatgg cggtgcacaa gcgggcaatc cacaaggcca actgagcgtg gcctgatcta
      421 catgaaacgg cgcgcttcgg cgcgccgttt cgtttccagg acgataatcg cgacatgaaa
      481 acgatctgcg tgtactgcgg ttccaatgcc ggcaacgatc cggcttacgc cgcgcaagcg
      541 gaagcactcg gcgcacgcct cgccgctgac ggcatcgccc tggtgtacgg cggcggcaac
      601 gtcggcctga tgggcatcgt cgcggacgcc gtgctggcga acggcggtga ggtcatcggc
      661 gtgatcccgc agcaactggt cgactgggaa gtcgcccatc gcggcgtcac ccgcctcgaa
      721 gtcgtcgatt ccatgcatac gcgcaaggcg cggatgttcg agctgtccga tgggttcgtc
      781 gccctgcccg gcggtttcgg cacgctggac gaaatgttcg agatgctgac ctggcggcaa
      841 cttggcctgg gcaagaagcc ctgcgccttc ctcgacgtca acggcttctg gcagcccttg
      901 atggcgatgc tggacaccat ggtgcgcgag cgcttcctgc acgccgaaca gcgcgacgac
      961 ctttggcatg gcgaggacat cgacgccctg cttgcatgga tgcgcggcta cgtgccggcg
     1021 caggccgaca aatggctgga cgagaagcgt cgcagccaac tcaagctcac gccggaggat
     1081 gtatgagcgt tcccgcgaat ttccgcgcgt tccgcatcca cgacgacgcc cagggctacc
     1141 gcagcggcat cgaggcgatt tcgctggacg acctcaatgc cggtgaagtc atcgtcaagg
     1201 gcgcgtattc ctcggtcaac ttcaaggacg cgctggccgg taccggccag ggcaagatcc
     1261 tgcgcaggtt cccgctggtc ggcggcatcg acgtggccgg ccacgtggtc gcctcgaccg
     1321 acccggcctt caaggagggc gaagcggtcc tggtcacggg cagcgggctg tcggagacgc
     1381 gcgacggcgg ctacgccgag tacgcgcgac tggaatcgaa gtgggtcgtc gccctgccga
     1441 ccgggctcag cttgcgcgag tcgatgatcc tgggcaccgc cggcttcacg gcggcgctgg
     1501 cgctgctgcg catggaagac aaccggcagt cgccgtcgtt ggggccattg gcggtcaccg
     1561 gcgccaccgg cggcgtgggt tcgctggcga tcgcgatctt cagcaaggcc ggttacgacg
     1621 tgcacgcgat cagcggcaag gccggccagg ccgggtatct gaagtcgctc ggcgcgagcg
     1681 aggtgctcgg gcgcgacgcg ctggccacga cccgcccgat ggacaccgcc cgcttcggtg
     1741 gcggcctgga caacgtgggc gggccgatgc tggccgcact gctggcgcag accgtgccgt
     1801 acggcaacgt ggcgaccgct ggcctggccg ccagccacga actgcatgcc acggtgatgc
     1861 ccttcatcat ccgcggcgtg tcgctgctcg gggtggcctc ggccggcacc gcgcgcgacc
     1921 tgcgcgacga ggtctggcgg cgcctttcgt cggactggaa gcccgctggt ctggaggcga
     1981 tctgcacccg cgaaaccacg ctggacggac ttcccggggt cttcgccacg atgcttgcgg
     2041 gcgattccct gggccggacc gtggtgcgcc tcgcggaatc ggcctgaccg gcttgcgggc
     2101 attcacgcgc ccgctacaat cggcacaaac acggggaact ccatggcacg catcctgatc
     2161 gtcgacgact cgccctccca attgatgggc atccgccgca tcgtcgaaaa gctgggccac
     2221 gacgcactga ccgccgagga cggcgccgcc ggcgtggaag ccgcgaagcg cgaaatcccg
     2281 gacctgatcc tgatggacgt ggtgatgccg aacctcaacg gcttccaggc cacgcgctcg
     2341 atcacccgcg agcccaccac caagcacatc ccggtgatcc tggtgaccac caaggaccag
     2401 gacaccgacc gcgtctgggg catgcgccag ggcgcgaagg cctacatcac caagcccttc
     2461 agcgaatccg agctctcgga actgatcgtc cagtacctgg cctgagccct gcggcagccg
     2521 caattccgcg gcaggctgaa cggttcgcga caacgaagac gccggcgaaa tgccggcgtt
     2581 ttcgtgtctg ctgccaggcc aaccggctcc cgctcaggcg cgccgccagt cgtcgccgaa
     2641 ggcgtcccgc atcgtcgcgt aggccgcgcg ctgggcatcg tcatgcgcgc gcggggcgag
     2701 gatttccagc tcgacgatct ggtcgccatc cgcgccctgc gtgccacccg gcaggcccct
     2761 tccacgcagg cgcagcttgc ggccggcctc ggaatccgcg gggatcttga gttccaccga
     2821 gccgcccagg gtcggcacgc tgagcgtcgc gcccagcgct gcttcccagg gggccaccgg
     2881 gagcacgtgg atcacgttgc ggccatcgac ctcgaaccgt gggtgcgcgg catattcgat
     2941 ctccagcagc aggtcgccgc cgttgttgcc ttgccgcgcg agccggatcg actgcccggg
     3001 cttgatgccc ttgggcacgc gtacctcgag ggtgcgcccg ttgacgccga tgcgcacgtt
     3061 gccgccggca tacaccgact ccagcggcac cgcgagcttt gcgcgggtcg tgccgtcgcg
     3121 cggctgccca ccggcctgcg gcccgcgcgc accggcaccg gcacggccgc gcccgaacat
     3181 gctttcgaaa aaatcggaga agccaccgcc ggcgccaccg ccggcgaaga tctcgtcgaa
     3241 atcggggtgt ccgcctggcc cgccgcggaa accaccgggg ggcggtcgca cctggtcgcc
     3301 cggccgatac ccgccggccc gcaactggtc gtaggccttg cgcttctccg ggtcgcgcag
     3361 cgcctcgtag gcctcgttga ccgccttgaa cttgtcctca gcgccaactt ccttgctgac
     3421 atcgggatgg aacttgcgcg ccagccgccg atacgcggtc ttgatctcgg catcgcctgc
     3481 gctgggctcc acgcccagca ccccgtagta atccttgaat tccatgcgcg ctccctgccg
     3541 ccaatcgcgg acgaggttaa ccgatcgccc tatgcacgac aggtagactg gcgcacccag
     3601 ccaagatgag gtcccgccat gtcgattcaa cccggcgaac gcattcccga ggcgatcctg
     3661 cagtacgtcg ccgatggcgt gcacaagatc gacaccctca ccctgttcga gggccgcaag
     3721 ctggtgctgt tctcggtgcc gggcgcgttc accccgacct gttcggaacg ccacctgccc
     3781 ggcttcgtcg agcacttcga cgcgttccgc gagcgcggca tcgaggtcgc ctgcatggcg
     3841 gtcaacgatc cgttcgtgat gcaggcctgg ggcgaaagcc agcacgtccc ggccggcatg
     3901 cgcatgctcg ccgacggcaa tgccgacttc acccgcgcac tgggcctgga gatggacgcc
     3961 agcggcttcg gcatgggcac gcgcagcaag cgcttcgcgc tgtacgccga ggacggcgtg
     4021 gtgcgcgagc tgttcgtgga agcgcccggc gagttccgcg tctcgtcggc ggagaacgta
     4081 ctggcgcacc tgccgggctg atgcggtcgc cgtgccggcg gctcagcgcg ccggcgccag
     4141 catgctgaag cggatgccgt gccatgcgcc ggaatccgcc agcgcggtca gttcatgcgc
     4201 gccgggcgtg tcgaagacgt gcaccagcga acggccgcca tcggtttcgc cgatccagcg
     4261 gccgtccagc aaccattgca cgcgaccctc gccgcccagc gcacgcaann nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt gccgcggcag gctgtcgacc acttcgaaca
     4441 gcaacggcag cgcggtcacc gcgccgtatt ggccgggcat cggcgtgcca tccggacgcc
     4501 ccacccacac acccaccgtg taacggcgcg tcccgccgat cgcccaggca tcgcggtagc
     4561 catagctggt gccggtcttc catgcaacgc gcgggcgccg gccggtgtcg aaggtgccct
     4621 cgctttcgcc gggacgcgga ttggcggcca gcacctcgcg cacgatccac gccgcgccgg
     4681 gcgacaacag gcggcgctcg atgcgcggat ccgcaggcga atagcgcacg cggccggcga
     4741 tgccgtcgcg atgcggcgcc gcgaaggcac cgaccaggtc ctcaagccgc gcgccggtgc
     4801 cgcccaggat catcgacagg ttgggcgacg cgccacgcgg gaatttcagg gcgatgccgg
     4861 catgttccaa ccgcgcggcg aaccgcgccg gcccgacgcg ttccagcagg tccaccgccg
     4921 gcacgttgag cgacagccgc agcgccgtgg ccgctcccac cgggccgttg aaggcggcgt
     4981 cgaaattgga gggccggtag ccgccgaacg cctgcggcgc gtcgaccaac aggctttccg
     5041 aatggatcat cccggcgtcc agcgccatgc cgtacaggaa cggcttgagc gtggagcccg
     5101 gcgagcgcca cgcctggacc atgtcgacat ggccgaggcg cagcttgtcg ccgaacgcca
     5161 ccgatccgac ataggcgcgc gcttccagcg tggcgttatc gaccaccagc agggctgccg
     5221 acgtgcgcgg cggcaggtcg gagaaatacg tggatacgcg ctcctccagg gtgcgctgca
     5281 acgaggcatc cagcgtggag gcgatgcgtt ccgccttcgg gtaggtggtg cgcaggcgct
     5341 gggccagcaa ggccgcgtcc atcggcggcc tcaggcggcg cgagacaacg ccctcgatgc
     5401 gtgcgtcgtc gacctgcgct cgtgtccacg tgccgctgct cgccatccgc gccagcagct
     5461 tgtcgcgcgc ctggcgggcg gcgtcgggcg agcggtcggg acgcagtcgg cttggcgcct
     5521 gcggcagcac ggcgagcagc gcagcctccg catgcgacag ccgcgcggcg ggcttgccga
     5581 ggtaggccca actcgccgct tccacgcctt cgatggtgcc gccgaacggc gcgcgttcca
     5641 ggtagagggt gaggatctcg tgcttcgaca gatgcgcttc cagttgcacg gcgcgcagcg
     5701 cctggcgcag cttgcccagc ggtgttcgcg tgcgcgatcc acgcaggccg ggatcgagga
     5761 tcctggccac ctgcatggtc agcgtcgagc cacccg
//
LOCUS       DAOOCX010000138         5793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13053_length_5793_cov_8.417916, whole genome shotgun sequence.
ACCESSION   DAOOCX010000138 DAOOCX010000000
VERSION     DAOOCX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13053_length_5793_cov_8.417916"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PK399_10615"
     CDS             complement(<1..1211)
                     /locus_tag="PK399_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HPW13612.1"
                     /translation="MEGLARIGFGLFGLAVLIGIAWLFSNNKKAVDWKLVLTGLGLQI
                     AFAAFVLLVPSIPISETRHIPGGREIFDTLGQGFVKILSFVGAGSNFIFGSLMDTTKF
                     GFVFAFQVLPTIIFFAALMGVLYHLGVMQFIVRIMALAITKVMRVSGAETTSVCASVF
                     IGQTEAPLTVRPYIPRMTESELLTMMIGGMAHIAGGVLAAYVGMLGGGDPAQQAFYAK
                     HLLAASIMAAPATLVVAKLLIPETGEPLTRGTVKMEVEKNASNIIDAAAGGAADGLKL
                     ALNIAAMLLAFIALIAMINWPLTWFGEVTGLQATLGKPTDLAAILGWVLAPLAWVIGV
                     PWQDATTVGSLIGQKVVINEFVAYLQLADIVNGKVAGVALTDQGRLIATYALCGFANF
                     SSIAIQIGGIGG"
     gene            complement(1290..2426)
                     /locus_tag="PK399_10620"
     CDS             complement(1290..2426)
                     /locus_tag="PK399_10620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW13613.1"
                     /translation="MIANSGWRDIWPRLLERPDALMLELGAGGELLVAQLRAGLSLVL
                     LLLPLVNVLTGEYSPSEMTAGMLGVVLAVVMSQVWLALARQRGRYRWLPWATSSYDVT
                     LTSLVLALLAMGSPATGLNSMVVWVFYLVAICMTALRNDGRLTIAIGLLAMLQYGALA
                     VAVFALADAPEQLMSLDYGTATVANTVQRVVLIAMMTAITSTVVYRMQRLVDLSGTDG
                     LTGLPNRTWLAHRFPGMLETTRGQGGSLSICLLNLDHFKRINDEIGHQAGDRALRHAV
                     DVLQEQLEEGDFLSRLGGEEFALLMPLPIGRAWERLDSMRRTTVSQPFLADPGADPIR
                     VTFSAGIAGWPQDGADLSQLLRRADVRLRQAKLAGRNRVLARDP"
     gene            complement(2625..4055)
                     /locus_tag="PK399_10625"
     CDS             complement(2625..4055)
                     /locus_tag="PK399_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPW13614.1"
                     /translation="MTPASKILALAIGLAVAGLAIAQTPPTPAQRKELDAARTQLDQA
                     AQRYAELSRKYGDADAPIRIEKRVLRKPVIGVVLAADDRSGVRIAAVTPGSAAADAGL
                     KTGDRITSVDGKRVDAGAGDARVEQARALLAALDAKTPVRLGYEREGKPATLSLTPKV
                     DDRVMVMQGPGGAAFDGDVQVFVGDEGGMRRIDADRIRLEAGAARAHAVEARARAAGE
                     RERAEWVMVAPDVRTEVIRLGSDCKGDDCKLPALAEAFRWNGLNLASVDAGLGRYFGA
                     SKGVLVLSTGKELDGLQAGDVIRSIGGKDVATPREAMDALRAQPAGGKVAIGYLRDRT
                     PATAQVNVPKAMTFALPRVAPAPRMPGEPGAIEHRKLVFVGTDGKMQAFEDGDGLVPR
                     ALPEDAKRVEKRRYVMVDKDGKRMEWEGAAGEPAPAWVQALPKDASQRQVRKIVVVDA
                     NGTATEVETDDLPPPPAPPAPPPRSR"
     gene            complement(4091..4864)
                     /locus_tag="PK399_10630"
     CDS             complement(4091..4864)
                     /locus_tag="PK399_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13615.1"
                     /translation="MPDAGDFEKNSASASPDRWGDAFAALPVETPAAAGWERLQARLP
                     AGATPRVRRRWPPWLATAAALALVMVVPLRMLPPTKADPIAPAVEVSTASISTPSVVT
                     VAPAVSTPIDADPPPAFVTVANRNKPSRPSRIAPAQRPVRTAAEPAGTTRLASEEAAR
                     QRIESLHADSAQLEQLLAMVRDERVANATTVALSSEFDARIAGIDDALAQSGLDAATR
                     DRLWQQRVDALQQLVGIETTNRLLSARGERFNASLVSID"
     gene            complement(4857..5465)
                     /locus_tag="PK399_10635"
     CDS             complement(4857..5465)
                     /locus_tag="PK399_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPW13616.1"
                     /translation="MAGHSSFAIDIPAALLARMRRGERDAFEQVYRRFERPVFTLALR
                     ICGDRAEADDVLQETMLKVWSSIDGFRGDGGTPFWGWLRQVAVNEALTRLRRGRRLAA
                     ELDGADTDAFAEDHAPPPPAAADAALLQRALEQLPHATRSVLWLYHAEGYTHEEIAAA
                     MQRTASFSKSQLARGTRRLRVLLQVSQPSVGTHTYRKEQAHA"
     gene            5571..>5793
                     /locus_tag="PK399_10640"
     CDS             5571..>5793
                     /locus_tag="PK399_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HPW13617.1"
                     /translation="MVQILALILLFAGIIILFKTVRMVPQGYEWTVERFGKYTHTMTP
                     GLHFLVPVMYGVGRKINMMEQVMDVPSQDV"
BASE COUNT          899 a         1951 c         2017 g          926 t
ORIGIN      
        1 ccgccgatgc cgccgatctg gatcgcgatc gagctgaagt tggcgaagcc gcacagcgcg
       61 taggtcgcga tcagccggcc ctggtcggtc agcgccaccc cggcgacctt gccgttgacg
      121 atgtcggcca gctgcaggta cgcgacgaat tcgttgatga cgaccttctg cccgatcagc
      181 gagccgaccg tggtcgcgtc ctgccacggc acgccgatca cccacgccag cggcgccagc
      241 acccagccga ggattgcggc gaggtcggtg ggcttgccca gcgtcgcctg caggccggtg
      301 acttcgccga accaggtcag cggccagttg atcatcgcga tcagggcgat gaacgcgagc
      361 agcatcgcgg cgatgttgag ggcgagcttg aggccgtcgg cggcaccgcc ggcggcggca
      421 tcgatgatgt tgctggcgtt cttctccact tccatcttga ccgtgccgcg ggtgagcggc
      481 tcgccggtct ccgggatcag cagcttcgca accaccagcg tcgccggcgc ggccatgatg
      541 ctggccgcca gcaggtgctt ggcgtagaag gcctgctgcg ccgggtcgcc gccgccgagc
      601 atgcccacgt aggccgccag cacgccaccg gcgatgtgcg ccatgccgcc gatcatcatg
      661 gtgagcagtt cggattcggt catccgcggg atgtacgggc gcaccgtcag cggggcctcg
      721 gtctggccga tgaacacgct ggcgcagacg ctggtggttt cggcgccgga cacgcgcatc
      781 accttggtga tcgccagcgc catgatccgc acgatgaact gcatcacccc gaggtggtac
      841 agcacgccca tcagcgcggc gaagaagatg atcgtgggca gcacctggaa ggcgaacacg
      901 aagccgaact tcgtggtgtc catcaggctg ccgaagatga agttcgagcc ggcgccgacg
      961 aagctgagga tcttcacgaa gccctggccc agggtgtcga agatctcgcg gccaccgggg
     1021 atgtggcggg tttcgctgat cgggatgctg ggaacgagga gcacgaacgc ggcgaaggcg
     1081 atctgcaggc ccaggccggt cagcacgagt ttccagtcga ccgccttctt gttgttcgag
     1141 aacagccagg caatcccgat cagcaccgcc agtccgaaca ggccgaagcc gatccttgcc
     1201 aaaccttcca tatgaccctt ccccgtccgt gcggacccgc acgcgatccc gcagggtagt
     1261 catgcccgcc ggtgccggca agcgggggct caggggtcgc gggcgagcac gcggttgcgg
     1321 cctgccagct tggcctggcg caggcgcacg tcggcgcggc gcagcaattg cgagagatcg
     1381 gcgccgtcct gcggccagcc ggcgatgccg gcgctgaagg tgacccggat cgggtcggcg
     1441 ccgggatcgg ccaggaatgg ctgcgagacc gtggtcctgc gcatgctgtc caggcgctcc
     1501 caggcgcggc cgatcggcaa gggcatcagc agggcgaact cctcgccacc caggcgcgac
     1561 aggaagtcgc cttcctccag ttgttcctgc aacacgtcga cggcatggcg cagcgcgcgg
     1621 tcgccggcct ggtggccgat ctcgtcgttg atgcgcttga agtggtccag gttgagcagg
     1681 cagatcgaca gcgaaccgcc ctgtccccgc gtggtttcga gcatccccgg gaaacggtgg
     1741 gccagccatg tgcggttggg caggccggtc aggccgtcgg tgccggacag gtcgaccagc
     1801 cgttgcatgc gatagacgac ggtgctggtg atcgcggtca tcatcgcgat cagcaccacc
     1861 cgctggaccg tgttcgccac cgtcgcggtg ccgtagtcca gcgacatcag ttgctccggc
     1921 gcgtccgcca gcgcgaacac cgccaccgcc agcgcgccgt actgcagcat cgccaggagg
     1981 ccgatggcga tggtcaggcg tccgtcgttg cgcagcgcgg tcatgcagat cgcgaccagg
     2041 tagaacaccc acaccaccat gctgttgagg ccggtggcgg gcgatcccat cgcaagcagc
     2101 gccagcacca ggctggtcag ggtcacgtca tagctggagg tggcccaggg caaccagcga
     2161 tagcggccgc gctggcgtgc cagcgccagc cagacctgcg acatcaccac cgccaggacc
     2221 accccgagca tcccggcggt catctccgag gggctgtatt cgccggtcag cacgttcacc
     2281 agcggcagca acaggagcac cagcgacaat cctgcgcgca gctgcgcgac cagcagttcg
     2341 ccgccggcgc caagctccag catcaatgca tcggggcgtt cgagcagtcg tggccagatg
     2401 tcgcgccaac cggaattcgc gatcaacgat ggacctccct cgggtccccc agcatagggt
     2461 gcgttgtgga tgccctcaaa tcgggcgcaa ccatcgaccg atgacgatgc gcgatctgcc
     2521 cggcgtcacg tcgcgctcca ttgagtttta tcatcatcca cggacacgaa ccgctgcccg
     2581 gtcaagaaag aagccccgca ttgcggggct tcttcgtcag cggatcagcg cgagcggggt
     2641 ggcggcgccg gcggggcggg tggcggcggc aggtcgtcgg tctcgacttc ggtggcggtg
     2701 ccgttcgcat cgacgaccac gatcttgcga acctgccgtt gcgatgcgtc cttcggcagc
     2761 gcctgtaccc acgcaggtgc aggttcgccg gcagcgccct cccactccat ccgcttgccg
     2821 tccttgtcga ccatcacgta gcgacgtttc tcgacccgct tggcgtcctc cggcaacgcg
     2881 cgcggcacca ggccatcgcc atcctcgaag gcctgcatct tgccgtcggt gcccacgaag
     2941 accagcttgc gatgctcgat cgcgccgggt tcgccgggca tgcgcggcgc gggggcaacg
     3001 cgcggcagcg cgaaggtcat cgccttcggc acgttgacct gcgcggtggc aggggtgcgg
     3061 tcgcgcaggt agccgatggc gaccttgccg ccggcgggtt gcgcgcgcag cgcgtccatc
     3121 gcctcgcgcg gggtggcgac gtccttgccg ccgatgctgc ggatcacgtc gccggcctgc
     3181 aggccgtcca gttcctttcc ggtgctgagc accagcacgc ccttgctggc gccgaagtag
     3241 cggcccaggc cggcatccac cgaggccagg ttcaggccat tccagcggaa tgcctcggcc
     3301 agcgccggca gcttgcagtc gtcgcccttg cagtcgctgc ccaggcggat cacctcggtg
     3361 cgcacgtccg gggcgaccat cacccattcg gcacgttcgc gttcgccagc cgcacgggca
     3421 cgggcctcga ccgcatgtgc gcgggccgcg ccagcctcca ggcggatgcg atccgcgtcg
     3481 atcctgcgca tgccgccttc gtcaccgacg aacacctgca cgtcgccatc gaatgcggcg
     3541 ccgccaggtc cttgcatgac catgacccgg tcatcgacct tcggggtcaa cgacagcgtg
     3601 gcaggcttgc cctcgcgttc gtagccgagc cgcaccggcg tcttcgcatc gagcgcggcg
     3661 agcagcgcgc gggcttgttc gacacgcgcg tcgccggcac cggcatcgac gcgcttgcca
     3721 tccacgctgg tgatgcggtc gccggtcttc aggccggcat cggcggcggc gctacccggg
     3781 gtgaccgcgg cgatgcgcac gccggatcga tcgtccgcgg ccagcaccac gccgatgacc
     3841 ggcttgcgca acacgcgctt ctcgatgcgg atcggcgcat cggcatcgcc gtacttgcgc
     3901 gagagttcgg cgtagcgctg cgcggcctgg tcgagctgcg tgcgtgcggc atcgagttcc
     3961 ttccgttgcg cgggcgtggg cggggtctgg gcgatcgcca ggccggcaac ggccaggccg
     4021 atcgcgagcg ccaggatctt ggatgcgggt gtcatgggga cctcgtgggt ggatcgggtg
     4081 gaaggagacg tcagtcgatg ctgaccaggg aggcgttgaa gcgttcgccg cgtgcggaca
     4141 ggaggcggtt ggtggtttcg atgccgacca gttgctgcag cgcgtccacg cgctgctgcc
     4201 agaggcggtc gcgcgtggcg gcatccaggc cggattgcgc aagcgcgtca tcgatgccgg
     4261 cgatgcgtgc gtcgaactcg ctgctcagcg ccacggtcgt cgcgttggcc acgcgctcgt
     4321 cgcggaccat cgccagcagt tgctccagtt gcgcggagtc ggcgtgcagc gattcgatgc
     4381 gctgcctggc cgcttcttcg gaagcgaggc gcgtcgtgcc ggcgggttcc gcggcggtac
     4441 gcaccgggcg ctgtgcgggc gcgatgcggc tcgggcgcga aggcttgttc cggttggcga
     4501 cagtaacgaa agcaggtggc ggatcggcgt cgatgggtgt cgagactgcg ggtgccaccg
     4561 tgacgacgga gggcgtggat atcgatgccg tcgagacttc gactgcaggc gcgatcggat
     4621 cggccttcgt cggcggcagc atccgcaacg gaaccaccat caccagcgcc agcgcagcgg
     4681 cggttgccag ccacggcggc caacgcctgc gcacacgtgg tgtcgcgccg gcaggcaggc
     4741 gcgcttgcag gcgttcccag ccagcggccg ccggtgtttc gacgggcagt gccgcgaacg
     4801 catcgcccca gcgatcgggt gacgccgatg cgctgttctt ttcgaaatcg ccggcatcag
     4861 gcatgcgcct gctccttcct ataggtatgt gtaccaaccg agggctggga aacctgcagc
     4921 agcacgcgca gccggcgggt gccgcgcgcg agttgggatt tggagaagct cgcggtgcgt
     4981 tgcatggcgg ccgcgatttc ctcgtgggta tagccttctg cgtggtacag ccagagcacg
     5041 ctgcgggtgg catgcggcaa ctgttccagc gcgcgctgga gcagcgccgc gtccgccgcg
     5101 gcgggcgggg gcggtgcgtg gtcttcggcg aatgcatcgg tgtccgcacc gtccagttcc
     5161 gccgccagtc gacgcccgcg ccgcagccgc gtcagcgcct cgttgaccgc gacctgccgc
     5221 agccagcccc agaacggcgt gccgccatcg ccacggaagc cgtcgatcga ggaccacacc
     5281 ttcagcatgg tctcctgcag cacgtcgtcg gcctcggcgc ggtcgccgca gatgcgcaac
     5341 gccagggtga acacgggtcg ttcgaagcgg cggtacacct gctcgaacgc gtcgcgctcg
     5401 ccccgacgca tgcgtgcgag cagggcggcg gggatgtcga tcgcgaagct ggagtgtccg
     5461 gccattccct tgtctggatg cgcgagggcg gaaaagggtc gcaggcagtc ggcaatgtcg
     5521 tccgccacgg ccgggcctat actcgggccg tcttccgggg aggaattccg atggtacaaa
     5581 ttctcgcgct gatcctgctg ttcgccggca tcatcatcct gttcaagacc gtgcggatgg
     5641 tgccgcaggg ctacgaatgg acggtggaac gcttcggcaa gtacacccac accatgaccc
     5701 ccggcctgca cttcctggtc ccggtcatgt acggcgtggg ccgcaagatc aacatgatgg
     5761 aacaggtgat ggacgtgccc agccaggacg tga
//
LOCUS       DAOOCX010000139         5764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13150_length_5764_cov_9.921703, whole genome shotgun sequence.
ACCESSION   DAOOCX010000139 DAOOCX010000000
VERSION     DAOOCX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13150_length_5764_cov_9.921703"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /gene="murL"
                     /locus_tag="PK399_10645"
     CDS             complement(<1..1232)
                     /gene="murL"
                     /locus_tag="PK399_10645"
                     /EC_number="5.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-
                     glutamate epimerase"
                     /protein_id="HPW13618.1"
                     /translation="MSDWVFDRDTIRAFRFVRCGFDAATGIARLVYAFDDGPELVETV
                     TLPGAPFALDDARGAAAERALQLLHLIAGVSYYKAAVPGEMRIEGYDIDAATAALLET
                     VYLNGLGEFAYRNGLDLRGRIRFPHAGGAMVPATTLGLRSHALVAIGGGKDSLVSIEA
                     LRAEGVAQTVTWIGGSQLIAACAAHTGLPTLNIGRQLAPQLFDLNRLGAWNGHIPVTA
                     VNSAIMAFAAVVLGVDQVVFSNERSASYGSIIPGTGEVNHQWSKGWAFESAFGAHLQS
                     HVAADLQYYSLLRPLSELAVARQFAKTDRYDAHFSSCNRNFHILGERPTSRWCGVCPK
                     CHFVFLALAPFVPKVRLVGIVGRNLLDDPAQTAGFDALLEYQDHKPFECVGEGRESRA
                     AMAALAQRPEWREDALVAR"
     gene            complement(1229..3850)
                     /locus_tag="PK399_10650"
     CDS             complement(1229..3850)
                     /locus_tag="PK399_10650"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HPW13619.1"
                     /translation="MSIPPASHDHWLVLKFGGTSVSKRERWDTIGRLASERAASEHAR
                     VLVVVSALSGVTNELTAIANGDDVAGRVAALIERHRAFCADELGIDADAAIGERLRAL
                     DALRDDPRAATRTLDWQAEVLGQGELLSSTLGAAYLRAQGLDFGWCDAREWLDALPPQ
                     PNQTDWSQRLSVNCQWQADAGWRERFTAQPARLLLTQGFISRHQDGGTAILGRGGSDT
                     SASYFGALLGARRVEIWTDVPGMFSANPREVPDARLLTRLDYYEAQEIATTGAKVLHP
                     RSIKPCRDGNVPLCILDTGRPHLPGTRIDGDAAAVSGVKAISRRNGIVLVSMEGIGMW
                     QQVGFLADVFGLFKKHGLSVDLIGSAETNVTVSLDPSENLVSTDVLSALSADLAKVCR
                     VKIIVPCAAITLVGRGMRSLLHKLSEVWAMFGRERVHLISQSSNDLNLTFVVDEAAAD
                     GMLPKLHAALIDSGAMPVYETEVFGPRWREINGAIRPRPTPWWHAPRERQRLLELAES
                     GTPRYVYHLPTVRERARQLKAIAAVDHRFYAIKANSNPSILRTLAEEGFGLECVSLGE
                     LQRVFESVPGFDPKRVLFTPNFAPVSEYEAAFAMGVNVTVDNVELLRQWPAIFRGRTV
                     WLRIDLGHGDGHHAKVNTGGKEAKFGLSAQRVEEFMDAARDLGMRITGLHAHLGSGIE
                     TTAHWKLVVDELAGFARRIGTVEVLDIGGGLPIAYSADDEPFDLDAWAEGLAQIKAVH
                     PAFRLAIEPGRFLVAESGVLLTRATQVVEKDGVHRVGLDAGMNALIRPALYDAWHDIV
                     NLSRLDHDFDTDFDVVGPICESSDIFGNRVKLPADTAPGDAMVIADAGAYGFSMANTY
                     NLRALPAEDLLDEGAPE"
     gene            3926..4786
                     /locus_tag="PK399_10655"
     CDS             3926..4786
                     /locus_tag="PK399_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010375445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPW13620.1"
                     /translation="MPHRYLQLDVFAARAGTGNPLGVVFDAGPMDTATMQALAAWLNL
                     SETIFFLPPDPGADYRIRIFTPGSELPFAGHPSVGAAWTAVVHGLAIPRDGQLIQQCE
                     AGLLPVEISGDHANPRVAVRSPRAKHLQDHAPLAGLDAIAVPGQAAALWNNGPSWWMV
                     EAASAKALRGLKPDFSAIAAWTNATATTGVAMFALEPEADHDVVVRAFCPGDAVNVPE
                     DPVTGSANALVGALLHAQGWLPGVDGRYIASQGRELGRDGRVHVRVDDDGQVWIGGQV
                     QQVIAGTLDW"
     gene            4817..5698
                     /locus_tag="PK399_10660"
     CDS             4817..5698
                     /locus_tag="PK399_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010369373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPW13621.1"
                     /translation="MPRRYVQLDVFASRPGAGNPLAVVLDAEGLDDAAMQAIAKWTRL
                     PETTFVFPAVSDDATHRIRIFSPRREVPFAGHPSVGTAHALLDAGLIAPREGFVVQDG
                     IAGKLPLKVNGEGAERTIAVRTPRARVQEIATPDDPRLRAALAGLDLGALPPVLMDGG
                     RRWWLAELASETALRSATPNWDAIIALANATESMGLFVYARSDDPVYHFAVRAFVGAP
                     AQFEDAASGAANATLAAWLAERDALPDQPGGFYRVSQGREVGHDAIIELSIDAEGDVW
                     SGGRAVAVVTGMLDWPE"
BASE COUNT          920 a         1997 c         1944 g          903 t
ORIGIN      
        1 cgggcgacca gcgcgtcctc gcgccattcc gggcgctgcg caagcgcggc catcgccgcg
       61 cgcgattcac ggccctcgcc cacgcattcg aacggcttgt ggtcctggta ttccagcaac
      121 gcatcgaagc cggcggtctg cgccggatca tccagcaggt tgcgtccgac gatgccgacc
      181 agtcgcacct tcggcacgaa cggcgccagc gccaggaaca cgaaatgaca cttcgggcag
      241 actccgcacc agcggctggt cggccgttcc ccgaggatgt ggaagttgcg attgcagctg
      301 gagaaatgcg cgtcgtaacg atccgtcttc gcgaactggc gcgcgaccgc gagctcactg
      361 agcggacgca gcagcgagta gtactgcagg tcggcggcga catgcgattg caggtgcgcg
      421 ccgaaggcgg attcgaacgc ccaccccttc gaccactggt ggttgacctc gccggtgccc
      481 gggatgatcg agccgtagct ggccgagcgt tcgttggaga acaccacctg gtccacgccc
      541 agcacgaccg cggcgaaggc catgatcgcg gagttcaccg cggtcaccgg gatgtgtccg
      601 ttccaggcgc ccagccggtt gaggtcgaac aattgcggcg ccaactgccg gccgatgttg
      661 agcgtcggca gtccggtgtg cgcggcgcag gccgcgatca gctgcgagcc gccgatccac
      721 gtgaccgtct gcgccacgcc ctcggcgcgc agcgcctcga tgctgaccag cgaatccttg
      781 ccgccaccga tcgcgaccag cgcgtgactg cgcaggccga gcgtcgtcgc aggcaccatg
      841 gccccgccgg catgcgggaa gcggatccgt ccgcgcaggt ccaggccatt gcgatacgcg
      901 aactcgccca gtccgttgag gtacaccgtt tccagcaagg ctgcggtggc ggcatcgatg
      961 tcgtagccct cgatgcgcat ctcgccgggc accgcggcct tgtagtagct cacgccggcg
     1021 atcaggtgca gcagctgcaa cgcacgctct gcagcagcac cgcgcgcatc gtccagtgcg
     1081 aacggcgcac ccggcaacgt caccgtctcg accagctccg ggccgtcgtc gaaggcatag
     1141 acaagccgcg cgatgccggt ggcggcatcg aagccgcatc gcacgaagcg gaacgcgcgg
     1201 atcgtgtcgc gatcgaacac ccagtcactc attcaggcgc accttcatca agcaggtcct
     1261 ctgcgggcag cgcgcgcagg ttgtaggtat tggccatgct gaaaccatag gcgccggcat
     1321 cggcgatcac catggcgtcg cccggcgcgg tgtcggcggg cagcttcacc cggttgccga
     1381 agatgtcgct ggattcgcag atcggcccga ccacgtcgaa gtctgtgtcg aagtcatggt
     1441 ccagccgcga caggttcacg atgtcgtgcc aggcgtcgta cagcgccggg cggatcagcg
     1501 cgttcatgcc ggcatccagg cccacgcgat gcacgccgtc cttctcgacc acctgggtcg
     1561 cacgcgtcag cagcacgccg gattccgcga ccaggaagcg gcctggctcg atcgcgaggc
     1621 ggaacgcggg atgcacggcc ttgatctgcg cgagcccttc cgcccaggcg tcgaggtcaa
     1681 agggttcgtc gtcggcgctg taggcgatcg gcaggccgcc accgatgtcg agcacttcca
     1741 ccgtgccgat gcgccgcgcg aagccggcca gctcatcgac caccagcttc cagtgcgcgg
     1801 tggtttcgat gccgctaccc aggtgcgcat gcaggccggt gatgcgcatg cccaggtcgc
     1861 gcgccgcgtc catgaattcc tcgacgcgct gggcggacag gccgaacttc gcttccttgc
     1921 cgccggtatt gaccttggcg tgatggccgt cgccgtggcc caggtcgatg cgcagccaca
     1981 cggtgcggcc gcggaagatc gccggccatt ggcgcagcag ctcgacgttg tcgacggtga
     2041 cattcacgcc cattgcgaac gcggcttcat attcgctgac cggcgcgaag ttgggggtga
     2101 acagcacgcg cttgggatca aagccgggca ccgactcgaa cacacgctgc aactcgccca
     2161 gcgacacgca ttccaggccg aacccttcct ccgccagcgt gcgcaggatc gacggattcg
     2221 agttcgcctt gatcgcatag aagcgatggt cgaccgcggc gatcgccttc agctggcgcg
     2281 cgcgttcgcg caccgtcggc aggtgataga cgtagcgcgg cgttccgctt tcggccagct
     2341 ccagcaggcg ttggcgctcg cgtggtgcat gccaccacgg cgtggggcgc ggccggatcg
     2401 caccgttgat ctcgcgccag cgcgggccga acacctcggt ctcgtagacc ggcatcgcgc
     2461 cgctgtcgat cagcgcggcg tgcagcttcg gcaacatgcc gtcggcggcg gcctcatcga
     2521 ccacgaaggt caggttcaaa tcattcgacg attgcgagat caggtgcacg cgctcgcgcc
     2581 cgaacatcgc ccacacttcg gagagcttgt gcagcagcga gcgcatgccg cggccgacca
     2641 gggtgatcgc cgcgcagggc acgatgatct tgaccctgca gaccttggcc aggtcggccg
     2701 acaacgccga gagcacgtcg gtgctcacca ggttctcgga cggatccagc gacacggtga
     2761 cgttggtctc ggcggaaccg atcaggtcga ccgacaggcc gtgcttcttg aacaggccga
     2821 acacgtcggc gaggaagccg acctgctgcc acatgccgat gccttccatc gacaccagca
     2881 cgatgccgtt gcgccggctg atcgccttga cgcccgagac cgctgccgca tcgccatcga
     2941 tgcgcgtgcc cggcaggtgc gggcggccgg tatcgaggat gcacagcggc acattgccgt
     3001 cgcggcaggg cttgatcgaa cgcggatgca gcaccttggc gccggtggtg gcgatttcct
     3061 gtgcctcgta atagtcgagg cgggtcagca gccgcgcatc cggcacctcg cgcgggttgg
     3121 cgctgaacat gccgggcacg tcggtccaga tctccacccg gcgcgcgccg agcaaggcac
     3181 cgaaatacga cgcggaggta tccgagccgc cacgcccgag aatcgcggta ccgccatcct
     3241 ggtggcgcga gatgaagccc tgggtcagca gcaaccgcgc gggctgtgcc gtgaagcgtt
     3301 cgcgccagcc ggcatcggcc tgccattggc agttcaccga caaccgttgc gaccagtcgg
     3361 tctggttggg ctgcggcggc agcgcgtcca gccattcgcg cgcatcgcac cagccgaaat
     3421 ccaggccctg cgcgcgcagg taggccgcgc ccagcgtgga cgagagcaat tcgccctgcc
     3481 ccagcacctc ggcctgccag tccagcgtgc gcgtcgccgc gcgcggatcg tcgcgcaagg
     3541 cgtccagcgc gcgcaggcgt tcgccgatgg cggcatcggc atcgatgccg agttcgtccg
     3601 cgcagaacgc gcgatggcgt tcgatcaacg ccgccacgcg gccggcgaca tcgtcgccat
     3661 tcgcgatcgc ggtcagctca ttggtgacgc cggacagcgc cgacaccacc accagcaccc
     3721 gtgcgtgttc gctcgcggcg cgctccgaag ccaaccgccc gatggtgtcc caacgctcgc
     3781 gtttcgacac cgacgtgcca ccgaacttca gcaccagcca gtgatcgtgg gacgcggggg
     3841 gaatggacat gggccttggg gatgtgtgtg ggcgttggcg tggtgcattc tagccggcct
     3901 cccccgccca tgagccgcgt ccgccatgcc gcatcgttat ctgcaactcg atgtcttcgc
     3961 cgctcgtgcc ggcaccggca atccgctggg cgtggtgttc gatgccggcc ccatggacac
     4021 cgccacgatg caggcgctgg ccgcctggct gaacctgtcg gagacgattt tcttcctgcc
     4081 gcccgatccc ggcgccgatt accggatacg catcttcacc ccgggcagcg aattgccctt
     4141 cgccggccac cccagcgtgg gcgcggcatg gaccgcggtg gtgcacggct tggctatccc
     4201 acgcgatggc cagttgatcc agcaatgcga ggctggcctg ctgccggtcg agatcagcgg
     4261 cgaccacgca aacccgcgcg tcgccgtgcg cagcccgcgt gcgaagcatc tgcaggacca
     4321 cgcgccgttg gctggcctgg acgcgatcgc cgttcccggg caggcggccg cgctgtggaa
     4381 caacgggccg tcgtggtgga tggtcgaagc cgcatcggcc aaggcattgc gcgggctgaa
     4441 gccggacttc agcgccatcg ccgcgtggac caatgcgacc gccaccaccg gcgtggcgat
     4501 gttcgcgctg gaacccgagg ccgaccacga cgtggtggtg cgggccttct gccccggcga
     4561 cgcggtcaac gtgccggaag acccggtgac cggcagcgcc aacgccttgg tgggcgcgct
     4621 gttgcatgcg cagggctggc tgcccggcgt tgatggccgc tacatcgcca gccagggccg
     4681 cgaactcggc cgcgacggtc gcgtgcatgt ccgcgtggac gacgacggcc aagtctggat
     4741 cggcggccag gtgcagcagg tcatcgccgg caccctggat tggtaagctg cgcggccctt
     4801 gcccaagctg agcgccatgc cccgtcgcta cgtgcaactc gatgtgttcg cctcgcgccc
     4861 cggtgccggc aatccgctgg cggtggtgct ggatgccgag ggcctggacg atgccgcgat
     4921 gcaggcgatc gccaaatgga cgcggctgcc ggagaccacc ttcgtgtttc ccgccgtgtc
     4981 cgacgatgcc acccaccgca tccgcatctt cagcccgcgc cgcgaagtgc cgttcgccgg
     5041 ccatcccagc gtcggcaccg cgcatgcgct gctggatgcg ggattgatcg caccgcgcga
     5101 gggcttcgtg gtccaggacg ggatcgccgg caagttgccg ctgaaagtca acggcgaggg
     5161 cgccgaacgc accatcgccg tgcgcacgcc gcgtgcgcga gtgcaggaga tcgccacgcc
     5221 ggacgatcca cgcttgcgcg ctgcgctggc cggactggac ctgggcgcgc tgccgccggt
     5281 gctgatggat ggcggacgcc gctggtggtt ggccgaactg gccagcgaaa ccgccttgcg
     5341 cagtgccacg ccgaactggg acgcgatcat cgcactggcc aacgccactg agagcatggg
     5401 cctgttcgtc tatgcgcgca gcgatgaccc ggtctatcac ttcgccgtgc gcgccttcgt
     5461 cggtgcgccc gcgcagttcg aggacgcggc ctccggcgcg gccaacgcga ccttggccgc
     5521 ctggctcgcg gaacgcgatg cattgcccga ccaaccgggc ggcttctacc gcgtcagcca
     5581 gggtcgcgaa gtcggccatg acgccatcat tgaactcagc atcgatgccg agggcgatgt
     5641 ctggtcgggt gggcgcgcgg tggcggtggt gaccgggatg ctggactggc cggagtagtt
     5701 cgcagcctcg attgatcctg ccggaacgac gaaggggcct cgcggcccct tcgtttcatt
     5761 cact
//
LOCUS       DAOOCX010000140         5699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13348_length_5699_cov_5.604890, whole genome shotgun sequence.
ACCESSION   DAOOCX010000140 DAOOCX010000000
VERSION     DAOOCX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13348_length_5699_cov_5.604890"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK399_10665"
     CDS             <1..358
                     /locus_tag="PK399_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPW13622.1"
                     /translation="AIALTTDSSILTAIGNDYGYDYVFARQVEALGQSGDVLVAISTS
                     GNSPNVLRAADAARARGMQVVAFTGRGGGKLLSMSDICFRMPSDETPRIQEGHEFVGH
                     LLCALIEAGLYPRDAG"
     gene            348..1049
                     /locus_tag="PK399_10670"
     CDS             348..1049
                     /locus_tag="PK399_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPW13623.1"
                     /translation="MPADEAIVLAGGFGTRLRGIVDNVPKPLAPVAGRPFLAWLLDRL
                     ATSGMRRCILATGYMADVIEQRIGARWHGMDIAYSVEQEPLGTGGAIRLAATQLHGDA
                     AHVLNGDTWLEYAPAALESAARDAGTPMAIALARVDDVARYGAVDIDQGRVVGFREKG
                     EAGAGWINAGCYFLGADALAGLPAQEAFSFEQAVLHARTQSGEVAAFTNTAGFIDIGV
                     PEDYARAQLQFAGRM"
     gene            1046..1624
                     /locus_tag="PK399_10675"
     CDS             1046..1624
                     /locus_tag="PK399_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPW13624.1"
                     /translation="MNASHVRAPFLDPSADVQLAGTASPRKALFLDRDGVININHGYV
                     HTPDDTDWVPGIFELVADAHARGHGCIVVTNQAGIGRGLYDEAGFLAYTAWMHAQFAA
                     RGTPLLATFFCPHHPDAGIGEYRADCDCRKPRPGMLVAAIARFGIDPSLSIMIGDKQG
                     DLDAAAAAGVPARLLREEQWGAEFARMLGTQS"
     gene            1621..3720
                     /locus_tag="PK399_10680"
     CDS             1621..3720
                     /locus_tag="PK399_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13625.1"
                     /translation="MSTQAQARPAWSWLLLAPLPILLVAFLRYRLGDHIDIDYRWMLA
                     AFALVQSLLAAVALQAAARGSRWTWVFPLVAAMPAMVGVVVFAGPLVSLAGLLLALAA
                     LGIGSRIADGDSSPAIMLLVGLAVIAALVGWLLPFPLHTRWAYLLVAALSIAWRARAL
                     IGLLGDAATSLSDGAARHPAWSILAIAAATVASLGLWLPSLNYDDNAVHLILQSQLLA
                     DGYYHLDVQSQSWAVAPWANNVLHAVAAMLAGQEARAAMGALWLLVGIDGARRLALAL
                     GGSPAVALAAAAMFAAQPFAGYFTTTMQVDGASAAILLQLAAIAAMPAAQRPGPLAIG
                     AIGGLLLALKTINLAFASPLLLWLAWSSMPGLRLRWTLHMLAILLPLAASSYCYAVLV
                     TGNPLFPFFNAVFQSPLYPLENFRDTKWMAGVTWRAPWDLAFHAERYGQYYAGASGLA
                     VLATLPALLVDAVRRRAPRWLALWALACAVLLFLQMQYLRYLFPAYAVLAVIGVVALA
                     RLLDWRGFVVAMAVLVAVDAALMPTTSWIVRANPWAALLREGPASRDALIAELMPERA
                     LLERIMAREPDACVLMTDPRRPFVGVGRGRALSMHRRYDPELWRARNAAEADPSGQAW
                     RTLLVRIGASHVVVDPANAPVLAKTLAAMGYVVLDRERSIEVRAPASEGMRRCSPGLQ
                     SSRDRSGTLSPMQEEKP"
     gene            3717..4625
                     /locus_tag="PK399_10685"
     CDS             3717..4625
                     /locus_tag="PK399_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13626.1"
                     /translation="MTTVACPVCRAGRSSLHASIDGHPYFSCAGCGSIHIDPKVIDML
                     DGGGSLVGDYTEEYWEQERVGAIERASGVSLCRAGEAVLYCRRPVRRFLDLGAGPGFL
                     LQELHRLIDPSASIFHGVEKYPPPYAAAGPNFHVGGIEVLQDRFDAGVCIEVVEHLTP
                     RMLDRMLSDLALVSEPGSLWLFNTGMPEYVRNEDPAYLDPTHRGHIISYSLAGVTPYF
                     QAHGFRVSALPGRSFGFFVEYQPTEDPPFDVRLYQPVAENLALLQRNGLLHQASFEAA
                     RSYYYQSEYLVRTQWALSLKAQLQWG"
     gene            4839..5684
                     /locus_tag="PK399_10690"
     CDS             4839..5684
                     /locus_tag="PK399_10690"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW13627.1"
                     /translation="MDFFVIEMAGWIESAGRPLRAVSVGAGDCAHEIDVAKGLLARGS
                     EDFSILCLDISEGALGRAGDAIHAQGLEGRVATMVHDFNQGLPDGDFDVVIANQSLHH
                     VVELERLFDSIVDRIGDAGCFLISDTIGRNGHLRWPEAKELVDEAWQWMPPGYRYNHR
                     LQRQEDDFLDWDCSQEGFEGIRAQDILPLLLERLQPSVFLAWGNIIDVFIDRGFGHHF
                     RRQTDWDLHFIDRVQAMDHAAIASGRISPTHMLARFQKTAERCVHEVGIGPEQALRRP
                     TASTS"
BASE COUNT          812 a         1856 c         1968 g         1063 t
ORIGIN      
        1 ggcgatcgcg ctgaccaccg acagcagcat cctcaccgcg atcggcaacg actacggcta
       61 cgactacgtg ttcgcgcgcc aggtcgaggc gctgggccag tcgggcgatg tgctggtcgc
      121 gatctccact tccggcaatt cgcccaacgt gctgcgcgcc gccgacgccg cgcgcgcgcg
      181 cggcatgcag gtggtcgcgt tcaccgggcg cggcggcggc aagctgctgt cgatgtcgga
      241 tatctgcttc cgcatgccgt cggacgaaac cccgcgcatc caggaaggcc acgaattcgt
      301 cggccacctg ctgtgcgcgc tgatcgaagc cggcctctac ccgcgcgatg ccggctgacg
      361 aggccatcgt cctcgccggc ggcttcggca cgcgcctgcg cggcatcgtc gacaacgtcc
      421 ccaagccgct cgcacccgtt gctggccgcc cgttcctggc gtggctgctc gatcggttgg
      481 ccacgtccgg catgcgccgc tgcatcctcg ccaccggcta catggccgat gtcatcgagc
      541 agcgcatcgg cgcgcgctgg catggcatgg acatcgccta ttcggtggag caggaaccgc
      601 tcggcaccgg tggcgcaatc cgcctggccg cgacgcaatt gcacggcgat gccgcgcatg
      661 tactgaacgg cgatacctgg ctcgaatacg cccctgccgc actcgaatcc gccgcgcgcg
      721 acgccggcac gccgatggcg atcgcgctgg cgcgcgtgga tgacgtggcg cgctatggcg
      781 ccgtcgacat cgaccagggc cgcgtggtcg gcttccgcga gaaaggcgag gccggcgctg
      841 gctggatcaa tgccggctgc tatttcctcg gcgccgatgc gctcgcggga ttgccggcgc
      901 aggaggcgtt ctcgttcgag caggccgtgc tgcacgcgcg tacgcagtca ggcgaagtcg
      961 ctgcgttcac gaacacggcg ggcttcatcg acatcggcgt gccggaggat tacgcgcgcg
     1021 cgcagctgca gttcgcgggg cgcatgtgaa cgcttcacac gtccgcgcgc cattcctcga
     1081 tccgtctgcg gatgtgcagc ttgcagggac ggcgtcgcca cgcaaggcgc tgttcctgga
     1141 tcgcgatggc gtgatcaaca tcaaccatgg ctacgtgcac acgcccgacg ataccgattg
     1201 ggtgccgggc atcttcgagc tggtcgccga tgcgcatgcg cgcggccacg gttgcatcgt
     1261 ggtcaccaac caggccggga tcggccgtgg tctttacgac gaggccgggt ttctcgccta
     1321 taccgcctgg atgcatgcgc aattcgcggc gcgcggcacg ccgttgctgg cgacgttctt
     1381 ttgcccgcac catcccgacg ccggcatcgg cgagtaccgc gcggattgcg attgccgcaa
     1441 gccgcgaccg ggcatgttgg tggccgcgat cgcgcggttc ggcatcgatc catcgctttc
     1501 gatcatgatc ggcgacaagc agggtgatct cgatgctgcc gcggctgcgg gcgttccggc
     1561 acgactgctg cgcgaagagc aatggggcgc ggagttcgcg cgcatgctgg gcacgcaatc
     1621 gtgagcacgc aggcgcaggc acgtcccgca tggtcctggc tgttgctggc gccgctgccg
     1681 atcctgctgg tcgcgttcct gcgttaccgg ctgggcgacc acatcgacat cgactatcgc
     1741 tggatgctcg cggcgttcgc gctcgtgcag tcgttgcttg cggccgtcgc gttgcaggcg
     1801 gccgcgcgcg gcagccgctg gacctgggtg ttcccgctgg ttgccgcgat gcctgcgatg
     1861 gtcggcgtgg tcgtattcgc cggtccgctg gtgtcgcttg caggcctcct gctggcgctg
     1921 gcggccctgg gcatcggatc gcggatcgcg gatggcgaca gctcgcccgc gatcatgctg
     1981 ctggtggggc tcgcagtgat cgcggcattg gtggggtggc tcctgccgtt cccgctccac
     2041 acgcgctggg cgtacctgct cgtggcagcg ctgtcgattg catggcgcgc acgcgcattg
     2101 atcgggttgc tgggcgatgc ggccacctcg ttgagcgacg gtgcggcacg acacccggcc
     2161 tggtcgatcc tggcgatcgc ggcggcgacc gtcgccagcc tgggcctgtg gctgcccagc
     2221 ctcaattacg acgacaacgc cgtccacctg atcctgcagt cgcagttgct ggcggatggc
     2281 tattaccacc tggacgtgca gagccagtca tgggcggtcg cgccgtgggc gaacaacgtg
     2341 ctgcatgcgg tcgcggcgat gctggccggg caggaagcgc gcgccgcgat gggtgcgctg
     2401 tggctgctgg tcggcatcga cggtgcgcgc cgcctggcgc tggcgctggg cgggtcgcct
     2461 gcggtggcac tggccgctgc cgcgatgttt gccgcgcaac cgttcgccgg ttacttcacc
     2521 accacgatgc aggtggatgg cgccagcgcc gcgatcctgc tgcagcttgc tgccattgcc
     2581 gccatgcccg ctgcgcaacg cccggggccg ctcgcgatcg gcgcgatcgg cggcttgctg
     2641 ctggcattga agaccatcaa cctggcgttc gcctcgccat tgctgctctg gctggcgtgg
     2701 tcgtcgatgc cgggcctgcg cctgcgctgg acgctgcaca tgctggcgat cctgctgccg
     2761 ctcgcggcat ccagttattg ctatgcggtg ctggtgaccg gcaatccgtt gtttccgttc
     2821 ttcaatgccg tgttccagtc gccgttgtat ccgctggaga atttccgcga caccaagtgg
     2881 atggccggcg tgacctggcg cgcgccatgg gacctggcct tccacgcgga acgctacggc
     2941 cagtattacg caggcgcgtc cgggttggcc gtgctggcca cgctgcctgc cttgctggtc
     3001 gatgccgtgc gccgccgtgc accgcgctgg ctcgccctgt gggcgctggc atgtgcggtg
     3061 ctgctgttcc tgcagatgca gtacctgcgc tatctgttcc ccgcgtacgc agtgctggcc
     3121 gtcatcggcg tggtcgcgct ggcgcggctg ctggactggc gcggcttcgt ggtggcgatg
     3181 gccgtcctgg tcgcggtgga cgctgcgttg atgccgacca ccagctggat cgtgcgcgcc
     3241 aatccgtggg cggcgctgct gcgcgaaggc ccggccagcc gcgatgccct catcgccgag
     3301 ctcatgccgg agcgtgcact gctcgaacgc atcatggccc gcgagcctga tgcctgcgtc
     3361 ttgatgaccg atccgcgtcg gcccttcgtc ggcgttggcc gcgggcgcgc gctttcgatg
     3421 caccgtcgtt acgatccgga actctggcgc gcgcgcaacg ccgccgaggc ggatccgtcc
     3481 ggacaggcgt ggcgcacatt gctggtgcgc atcggcgcat cgcacgtggt ggtggatccg
     3541 gcgaatgcgc cggtgttggc gaagacgctt gcggccatgg ggtatgtggt cctggatcgc
     3601 gaacgttcga tcgaggtgcg tgctcccgcc agcgaaggca tgcgccgctg ctcgccgggg
     3661 ttgcaatcat cgcgggacag gtcgggtacg ctgtcgccca tgcaggaaga gaagccatga
     3721 cgaccgtcgc atgcccggtc tgccgtgccg gccgcagcag cctgcatgcc tccatcgatg
     3781 gccatccgta tttcagttgc gccggttgcg gttccatcca catcgatccg aaggtgatcg
     3841 acatgctcga tgggggtggc tcgctggtcg gcgactacac ggaggaatat tgggagcagg
     3901 agcgggtggg tgcgatcgaa cgcgcctccg gggtgtcgct ctgccgggcc ggtgaagcag
     3961 tgctgtattg ccggcgaccc gttcgccgat tcctcgacct aggtgccggc ccgggcttcc
     4021 tgctgcagga gttgcatcga ctgatcgacc cgtcggcctc gatcttccat ggcgtggaga
     4081 agtatccgcc gccgtatgcc gccgctggcc cgaatttcca tgtgggtggc atcgaggtcc
     4141 tgcaggatcg cttcgacgca ggtgtgtgca tcgaggtcgt cgagcacctg accccgcgca
     4201 tgctcgacag gatgctgtcg gacctggccc tcgtcagtga gccgggcagc ttgtggttat
     4261 tcaataccgg catgccggag tacgtacgca acgaggatcc ggcctacctg gatcccaccc
     4321 accgcggcca catcatcagt tactcgctgg cgggcgtgac cccgtacttc caagcccacg
     4381 gcttccgcgt gagcgcgttg ccggggcggt cgttcggatt cttcgtggaa taccagccga
     4441 cggaagatcc accgttcgat gtgcggcttt accagccggt ggccgaaaac ctggcgctct
     4501 tgcagcgcaa cggtttgctg caccaggcct cgttcgaggc ggcgcgctcc tattactacc
     4561 aatccgagta cctggtccgg acgcagtggg cgctgtcgtt gaaggcgcag ttgcagtggg
     4621 gatgaacgcg cttcgcggct gagctagggt cagggggcgc agggcgggcg catggcgtcc
     4681 agggggagtc aacgatgagc gaatattcag agcgtctgga acaggagcgt gcgcgtttcg
     4741 acgccgacct gtgcgtgcat gacctgccgg ccattttcca ttactgggcc aacgcgtatc
     4801 cgcgcgtgat gctcaacacc cacggttacg acaacatcgt ggatttcttc gtcatcgaga
     4861 tggccgggtg gatcgaatcg gctgggagac cgttgcgggc agtgagcgta ggtgccggcg
     4921 attgtgccca cgagatcgac gtcgcgaagg gattgctggc gcggggatcg gaggatttca
     4981 gcatcctttg cctggacatc agcgagggtg cgttggggcg tgcgggcgac gcgatccacg
     5041 cgcagggcct cgaaggccgg gtcgccacga tggtccacga cttcaaccaa ggcttgcccg
     5101 atggtgattt cgacgtcgtg atcgccaacc agtcgttgca ccacgtcgtc gaacttgaac
     5161 gcctgttcga ttcgatcgtc gaccgcatcg gtgatgcggg ctgcttcctg atctctgaca
     5221 cgatcggccg caacggccac ctgcgctggc cggaggcgaa ggaactggtc gacgaggcat
     5281 ggcaatggat gccgcccggg taccgctaca accaccggct gcagaggcag gaggacgatt
     5341 tcctcgactg ggattgctcg caggaggggt tcgagggcat ccgcgcccag gacatccttc
     5401 cgctcttgct ggagcggttg cagcccagcg tattcctggc ctggggcaac atcatcgacg
     5461 tgttcatcga ccgcggattc ggccaccact tccgccggca gacggactgg gacctgcatt
     5521 tcatcgacag ggtgcaggcc atggaccatg cggccatcgc atcgggccgg atcagcccga
     5581 cgcatatgct cgcccggttc cagaaaacgg ccgagcgctg tgtgcacgag gtggggatcg
     5641 ggcccgagca ggcgctcagg cgtccaaccg cctccacgtc ctgaccggat cacactgca
//
LOCUS       DAOOCX010000141         5698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13352_length_5698_cov_8.161793, whole genome shotgun sequence.
ACCESSION   DAOOCX010000141 DAOOCX010000000
VERSION     DAOOCX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13352_length_5698_cov_8.161793"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PK399_10695"
     CDS             <1..204
                     /locus_tag="PK399_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HPW13628.1"
                     /translation="AFGMFATPFIMFFVLWHSKKHGVVAEARAWEGAKGLEWTVPSPA
                     PHHTFTTPPVIGPGDLAHGDISH"
     gene            215..352
                     /locus_tag="PK399_10700"
     CDS             215..352
                     /locus_tag="PK399_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13629.1"
                     /translation="MTAGPDIASDNLDLRRRRARRTALLFGGIAVAVYVGFLLSGVVG
                     R"
     gene            349..927
                     /locus_tag="PK399_10705"
     CDS             349..927
                     /locus_tag="PK399_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HPW13630.1"
                     /translation="MSRSTAKSFGVLKLVGVSLAAFGFAFSLVPLYRIACEKVFGVRL
                     EQGPASASATAVAPKERWVTVQFDGGVNSKLPWQFAPAQASMRVQVGRQYEAEYSARN
                     TSDRAIVGNAVPSVAPARASGYFSKTECFCFTAQTLAAGESREMPVRFIVDPGLPAGV
                     KTITLSYTFFKNDTLTAALGAGQGHAGVHAAP"
     gene            959..>1857
                     /locus_tag="PK399_10710"
     CDS             959..>1857
                     /locus_tag="PK399_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HPW13631.1"
                     /translation="MANAHADLHHDDSHHGSHGQDVYFVPHGSKWPVFASVALFVTMV
                     GFASWLNEVSWGKATFYVGLAGLLAILFKWFGDVIRESLAGHYNKQVDTSFRMGMVWF
                     IFSEVMFFAAFFGALFYARQFALPWLNGEGDGGATHSILWSSFSGGWPSNGPGAIGGH
                     YQTIPAWGLPLLNTLILLTSGVTITIAHHALKEGKRGILLLFLGLTVLLGCTFLFYQA
                     EEYIHAYHELNLTMGSGIYGSTFFMLTGFHGAHVTLGTLMLAIMWLRCAKGHFSKDNH
                     FAFEAVAWYWHFVDVVWLGLFLFV"
     assembly_gap    1858..1957
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1989..2216)
                     /locus_tag="PK399_10715"
     CDS             complement(1989..2216)
                     /locus_tag="PK399_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HPW13632.1"
                     /translation="MSDSLKTLLIVGVLILILWNLGAGLYYMLVDKGQTKRTVNSLTK
                     RIGLSVALILFVVLAIKMGWITPHGVGANPN"
     gene            2274..3023
                     /locus_tag="PK399_10720"
     CDS             2274..3023
                     /locus_tag="PK399_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HPW13633.1"
                     /translation="MSPRALNLSLGWLLAVLAMAAFALAGRWQLDRMHQKEAMLAAAA
                     TALAQGQPQPLLLASDPGRREAYDRAEGAGRIANATVWLDNQMREGRSGVRMYCVLFA
                     DEGVQPMLLDAGWWPLDGRRTLPAFGCPAGDAQAVRGLLAPPPSTGIVHGEPMAPSGD
                     ARWLAARLDLPALSRALKLSTGLAPRVLRLDPERKTDDAGVMLAPGERDLDILANTLT
                     PQRHLGYAVQWFGLAIMVLVVALVLTLRKKR"
     gene            3020..3604
                     /locus_tag="PK399_10725"
     CDS             3020..3604
                     /locus_tag="PK399_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13634.1"
                     /translation="MMELTPAQRAIRRRNRNMLVGLFVLFFGGMLVAGLLRFSGWRPE
                     GSKNKGELLQPYGDLRAYTPTLANGGAYGWKDEPRIWRIVAMPRDCATSRAAECKRLL
                     ADFDKVWRLMSKDADRVHVLWAGDLPPGVAVPREVRQLRADDGLRAGLARWDDASGDA
                     AWLVDPNGFVVLRYAPGFDPGDLRSDLARLLKIN"
     gene            3610..4797
                     /locus_tag="PK399_10730"
     CDS             3610..4797
                     /locus_tag="PK399_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HPW13635.1"
                     /translation="MNSSTPAQRTTLNKPSLHKPALYRHFHRIAWLAVALASCVIVFG
                     AFVRLSNAGLSCPDWPTCYGKAAWPTHAEQIVDHIATEIRPVEPSKAWREQFHRHIAA
                     GLGLLVFALALLAARRRRFGIAQVLGAAALVAIAIPLYMQGQHVASSVLVAIAEAALL
                     WAALRWSNIDLARTAVLTLAVIIFQAMLGMWTVTWLLKPVVVMAHLVGGLTTFSLLTW
                     MAWRATDIPIQVAGAAKLRRLLIIGLLLLGVQIALGGWTSANYAALACGTDFPKCAGQ
                     WWPPHDFREGFVLWRGIGVDYEGGILDNQARIAIQLAHRLMAVLVFGHLLVLAIRLLR
                     SPGLRGWGSLLGTLLFVQVGLGIANVVMSLPLHVAVLHNAGATALLFVLVSLLARVRA
                     PDA"
     gene            4794..>5698
                     /gene="cyoE"
                     /locus_tag="PK399_10735"
     CDS             4794..>5698
                     /gene="cyoE"
                     /locus_tag="PK399_10735"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HPW13636.1"
                     /translation="MSKAVPSPGSQYWALTKPRVVALIVFTAIIGMLLAIQPGDEWPW
                     LRLLAGSIGIWLAAASAAAINHLLDQRIDKVMARTADRPLATGTLRPMQVLAFAIALG
                     VASMAILLLLVNAITAVLTFASLIGYAFVYTGFLKRATPQNIVIGGIAGAAPPLLGWA
                     AVTGMQNPRDWEYALLLVLVIFVWTPPHFWALAIFRREDYARALVPMLPVTHGVEYTR
                     WQILLYTVLLVVATILPWATHMSGVFYLGGALVLGAVFLWYAWKLLDPPDEFFAMRAF
                     KYSIVYLMALFAFLLLDHWLLPFLQP"
BASE COUNT          769 a         1912 c         1914 g         1003 t
ORIGIN      
        1 gcgttcggca tgttcgccac cccgttcatc atgttcttcg tcctgtggca ttcgaagaag
       61 catggcgtcg tcgccgaggc gcgtgcgtgg gaaggcgcga agggccttga gtggacggtg
      121 ccgtcgccgg ccccgcacca caccttcacc accccgccgg tgatcggccc gggcgacctt
      181 gcccacggcg acatcagcca ctgagcctga cccgatgacc gccggccccg acatcgccag
      241 cgacaacctc gacctgcgtc gccgccgtgc gcgccggacg gcgttgctgt tcggcgggat
      301 cgcggtggcg gtgtacgtgg gcttcctgtt gtcgggcgtg gtgggccggt gagccggtcc
      361 accgcgaaga gcttcggggt gctgaagctc gtcggcgtgt cgctggcggc gttcggcttc
      421 gcgttctcgc tggtgccgct ataccggatc gcctgcgaga aggtgttcgg ggtgcgcctg
      481 gagcagggcc cggcgtcggc cagcgccacg gccgtcgcgc cgaaagagcg ctgggtgacc
      541 gtgcagttcg acggcggcgt gaattccaag ctgccgtggc agttcgcgcc ggcgcaggcc
      601 agcatgcgcg tgcaggtcgg ccgccagtac gaagcggaat actccgcccg caacacctcc
      661 gaccgcgcga tcgtcggcaa cgcggtgccc tcggtggcac cggcgcgcgc ctcgggttac
      721 ttcagcaaga ccgagtgctt ctgcttcacc gcgcagacgc tggccgccgg cgaatcgcgc
      781 gagatgccgg tgcgcttcat cgtcgatccc ggcttgccgg ccggggtgaa gacgatcacc
      841 ttgtcctaca ccttcttcaa gaacgacacc ctgaccgccg ccctcggcgc gggccagggc
      901 catgccggcg tccacgcggc accctgacca cctcgatcca cgaaccatcg gaaccgccat
      961 ggcgaacgcc cacgcagacc tccaccacga cgacagccac cacggcagcc acggccagga
     1021 cgtctatttc gtcccgcatg gcagcaagtg gccggtgttc gcctccgtcg cgctgttcgt
     1081 caccatggtg ggcttcgcca gctggctgaa cgaagtgagc tggggcaagg ccacgttcta
     1141 tgtcggcctg gccggcctgc tggcgatcct gttcaagtgg ttcggcgacg tcatccgcga
     1201 gtcgctggcc ggccactaca acaagcaggt cgacacctcg ttccgcatgg gcatggtgtg
     1261 gttcatcttc tccgaggtga tgttcttcgc cgcgttcttc ggtgcgctgt tctacgcgcg
     1321 ccagttcgcg ttgccgtggc tcaacggcga gggcgacggc ggcgccaccc acagcatcct
     1381 gtggtcgagc ttcagcggcg gctggccgag caacggcccg ggcgcgatcg gtggtcacta
     1441 ccagaccatc ccggcgtggg gcctgccgct cctcaacacc ctgatcctgc tgacctcggg
     1501 cgtgaccatc accatcgccc accatgcgct caaggaaggc aagcgcggca tcctgctgct
     1561 gttcctgggc ctgaccgtgc tgctgggctg caccttcctg ttctaccagg cggaggaata
     1621 catccacgcc taccacgagc tcaacctgac catgggctcg ggcatttacg gttccacctt
     1681 cttcatgctc accggcttcc atggcgcgca cgtgaccctg ggcacgctga tgctggcgat
     1741 catgtggctg cgctgcgcga agggccattt cagcaaggac aaccatttcg cgttcgaggc
     1801 ggtggcctgg tactggcact tcgtcgacgt ggtgtggctg ggcttgttcc tgttcgtnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc cggcctttgt gtcttcgaag
     1981 caagtgactc agttcgggtt ggcgccgacc ccgtgcgggg tgatccagcc catcttgatc
     2041 gccagcacca cgaacaggat caaggccacg ctcaggccga tgcgcttggt cagtgaattg
     2101 acggtgcgct tggtctggcc cttgtccacc agcatgtaat acaggccggc gccgaggttc
     2161 cagaggatga ggatcaacac gccaacgatg agcagggttt tcagcgaatc gctcatgctt
     2221 gtctccgcga tgcggaccgc ccgcatgttc cgcgcattat cgcagctgcc gcgatgagtc
     2281 cgcgcgcgct caacctgtcg cttggctggc tgctcgccgt gctggcgatg gccgcgttcg
     2341 ccctggccgg ccgctggcag ctggaccgca tgcaccagaa ggaagcgatg ctggccgcgg
     2401 ccgccaccgc gcttgcgcag ggccagccgc agccgttgct gctggcatcc gatcccggcc
     2461 gccgcgaggc ctacgaccgc gccgaaggcg cggggcgcat cgccaacgcc accgtctggc
     2521 tggacaacca gatgcgcgaa ggacgcagcg gcgtgcgcat gtactgcgtg ctgttcgccg
     2581 acgagggcgt gcagccgatg ctgctcgatg ccggttggtg gccgctggat ggccggcgca
     2641 ccttgcctgc attcggatgc ccggcgggcg atgcgcaagc ggtgcgtggc ctgctggcac
     2701 ctccgccgtc caccggcatc gtccatggcg agcccatggc gccgtccggg gacgcgcgct
     2761 ggctggctgc gcggctcgac ctgccggcgc tgtcgcgtgc gctgaagctg tcgaccggcc
     2821 tcgcaccgcg cgtgctgcgg ctggatcccg agcgcaagac ggacgatgcc ggggtgatgc
     2881 tggcgcccgg cgagcgcgac ctcgacatcc tggccaacac cctgaccccg cagcgccacc
     2941 ttggctatgc ggtgcaatgg ttcggcctgg cgatcatggt gctggtggtc gcgctggtgc
     3001 tgaccctgag gaagaaacga tgatggagct gacacctgcg cagcgggcga tccgccgccg
     3061 caaccgcaac atgctggtgg gcctgttcgt gctgttcttc ggcggcatgc tggtggccgg
     3121 gctgctgcgc ttttccggct ggcgaccgga gggcagcaag aacaagggcg aactgctgca
     3181 gccgtacggc gacctgcgcg cctacacgcc gacgctggcg aacggcggcg cttacggctg
     3241 gaaggacgag ccgcgcatct ggcggatcgt ggcgatgcca cgggattgcg cgacgtcgcg
     3301 cgcggccgag tgcaagcgcc tgctcgccga tttcgacaag gtctggcggc tgatgagcaa
     3361 ggacgcggac cgcgtgcacg tgctgtgggc gggcgacctg ccgcccggcg tcgcggtgcc
     3421 tcgcgaggtg cgccagctgc gcgcggacga cggcctgcgc gccggcctgg cccgctggga
     3481 cgatgcgagc ggtgacgccg cctggctggt cgatcccaat ggtttcgtgg tgttgcgcta
     3541 cgctcccggc ttcgacccgg gcgacctgcg cagcgatctt gcccgcctcc tgaagatcaa
     3601 ctgatcccga tgaattccag cacgcctgcc caacgcacga ccttgaacaa gccgtcgctt
     3661 cacaagccgg ccctgtacag gcatttccat cgcatcgcgt ggctggcggt cgcgctggca
     3721 tcgtgcgtta tcgtgttcgg tgcgttcgtg cgcctgtcca atgccggcct cagctgcccc
     3781 gactggccca cctgctacgg caaggcggca tggccgacgc acgcggagca gatcgtcgac
     3841 cacatcgcca ccgagatccg cccggtcgag cccagcaagg cctggcgcga acagttccat
     3901 cggcacatcg ccgccgggct gggcctgctg gtgttcgcgc tggcgctgct ggccgcccgc
     3961 cgccggcgct tcgggatcgc ccaggtcctt ggcgcggccg cgctggtcgc catcgccatc
     4021 ccgctgtaca tgcagggcca gcacgtggcg tcgtcggtgc tggtcgcgat cgcggaagcc
     4081 gcgctgctct gggccgcgct gcgctggagc aacatcgacc tggcgcgcac cgcggtgctg
     4141 accctggcgg tgatcatctt ccaggccatg ctgggcatgt ggacggtgac ctggttgctg
     4201 aaaccggtgg tggtcatggc ccacctggtc ggggggctga ccacgttctc gttgctcacc
     4261 tggatggcat ggcgcgcgac cgacatcccg atccaggtgg caggtgcggc gaaactacgc
     4321 cggctgctga tcatcggctt gctcctgctc ggcgtgcaga tcgcgctggg cggctggacc
     4381 agcgcgaact acgcggcgct ggcctgcggt accgatttcc cgaagtgcgc gggccagtgg
     4441 tggccgccgc acgacttccg cgagggcttc gtgctatggc gcgggatcgg cgtggattac
     4501 gaaggcggca tcctcgacaa ccaggcacgc atcgcgatcc agctggcgca ccggttgatg
     4561 gccgtgctcg tgttcggcca cctgctggtg ctggcgatcc gcctgctgcg cagcccgggc
     4621 ctgcgcggct ggggcagcct gctgggcacc ttgctgttcg tgcaagtcgg gctgggcatc
     4681 gccaacgtgg tgatgtcgct gccgctgcat gtcgcggtgc tgcacaacgc cggcgcgacc
     4741 gccctgctgt tcgtgctggt gtcgctgctg gcgcgggtgc gcgcgcctga cgcatgagca
     4801 aggccgtgcc atcgcccgga tcgcagtact gggcgttgac caagccgcgc gtggtcgcgc
     4861 tgatcgtgtt caccgcgatc atcggcatgt tgctggcgat ccagcccggc gacgaatggc
     4921 cgtggctgcg attgctggcc ggcagcatcg gcatctggct ggccgcggcc agcgcggcgg
     4981 cgatcaacca cctgctcgac cagcgcatcg acaaggtgat ggcgcgcacc gccgaccgtc
     5041 cgctggccac cggcacgctg cgccccatgc aggtgctggc gttcgcgatc gcgctgggcg
     5101 tggcctcgat ggcgatcctg ctgctgctgg tcaatgcgat caccgcagtc ctcacgttcg
     5161 catcgctgat cggctacgcc ttcgtctaca ccggcttcct caagcgcgcc acgccgcaga
     5221 acatcgtgat cggcggcatc gccggcgccg cgccgccgct gctgggctgg gccgcggtga
     5281 cgggcatgca gaatccgcgc gactgggaat acgcgctgct gctggtgctg gtcatcttcg
     5341 tgtggacgcc gccgcatttc tgggcgctgg cgatcttccg gcgcgaggat tacgcgcgcg
     5401 cgctggtgcc gatgctgccg gtgacccacg gcgtggaata cacacgctgg cagatcctgc
     5461 tgtacaccgt gctgctggtg gtcgccacga tcctgccgtg ggccacgcac atgagcggcg
     5521 tgttctacct cggcggcgcg ctggtgctgg gcgcggtgtt cctctggtat gcgtggaagc
     5581 tgctggatcc gcccgacgag ttcttcgcga tgcgcgcgtt caagtactcc atcgtctacc
     5641 tgatggcgct gttcgccttc ctgctgctcg accattggct gctgccgttc ctgcagcc
//
LOCUS       DAOOCX010000142         5671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13419_length_5671_cov_8.410613, whole genome shotgun sequence.
ACCESSION   DAOOCX010000142 DAOOCX010000000
VERSION     DAOOCX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13419_length_5671_cov_8.410613"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..360
                     /locus_tag="PK399_10740"
     CDS             67..360
                     /locus_tag="PK399_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011259856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13637.1"
                     /translation="MSIFRNPLFTRFNRVRFASFGAPRRPRHPFLRLALGLVGVALLI
                     ALLFVSVFVGIAMLAAGMLFRLFRQRRAPMAARAGSIDADYRVVGKAQLPLAH"
     gene            361..1104
                     /locus_tag="PK399_10745"
     CDS             361..1104
                     /locus_tag="PK399_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPW13638.1"
                     /translation="MDSPAPGDLVAAAPTPRIPVRDRGGNLLGTFPVRRIYCVGRNFA
                     DHAREMGATAPASKAERGNPVFFMKPADAIVIDGVVPYPPGTSDLHHEVELVVGLGTD
                     APAGDLPLDVAASLVFGYGVGLDLTRRDLQAAAKAKGLPWDTGKGFDHSAPVSDLLPA
                     ADAGDIESLALSLDVNGERRQQSSLDQLIWNVPDILHELSKLYALRAGDLVFMGTPAG
                     VAALQAGDTFDARLGDIARLQGHIAQAAV"
     gene            1143..1547
                     /gene="mscL"
                     /locus_tag="PK399_10750"
     CDS             1143..1547
                     /gene="mscL"
                     /locus_tag="PK399_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPW13639.1"
                     /translation="MGMMTEFKEFAMRGNVIDLAVGVVIGAAFGKIVTALVDKVIMPP
                     LGLLIGGVDFSKMAIVLKDATVDAAGKDVPAVVLAYGEFINALVQFVIVAFAIFLVVK
                     AINRMHKKPEEAPAATPEDVLLLREIRDSLKK"
     gene            1623..3029
                     /locus_tag="PK399_10755"
     CDS             1623..3029
                     /locus_tag="PK399_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPW13640.1"
                     /translation="MRRTLLAAALSLLVALPLQAQQPTRIGDAAMTQAVTLRERALAD
                     DTGWKVVESLTTEVGPRLAGSEADARAVEWAKAKFKALGYDKVWTEPVTFPKWERRSE
                     SARIVGAHAQALHVTALGGSPGGKVEAEVVRFESLEALEKAAPGSLAGKIAFIDYRMT
                     RAKDGRGYGPGSRVRSGGPSAAIRAGALAYVMRSAGTDSHRNPHTGITRFDDGLKPIP
                     SAALSLPDADQLARLVALGQVKIALDLDCGWNGEYTSQNVIGEITGRSKPDDVVVIGG
                     HLDSWDLGTGAIDDGAGVAIGMAAGHLIGTLPKGQRPARTIRVVAFANEEQGLYGGRA
                     YAQKHAADLRKHQLGAESDFGAGRIYAFAAKTPDYAKEAVARIAQALAPLGIEHREDG
                     EGCGPDLSPFNAIGMACAGLSQDGTDYFDLHHTPDDTLDKIDPKALAQNVAAYTVFAF
                     LAADAQGDFGSAPKPATP"
     gene            3134..5131
                     /locus_tag="PK399_10760"
     CDS             3134..5131
                     /locus_tag="PK399_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HPW13641.1"
                     /translation="MHIRCILWLLLCAFSSFAVAGESTPLRLDSPGGNASVEVSLDPQ
                     GQPHYAIAWRGMPVLLPSPLGVQLGGDDRIERGLRVIDTTRSSTDSSYELVAGKTRHV
                     RDHHRQLDITFADAQGRRLGLIVRAYDDGIALRYRLPLPPQGDLVVKEDLTGFLFPRN
                     LACHALNLGRFGTSHEGEYDPIPASLIRPHHLIELPLVCGTGTGTTTFAIAEADLDRY
                     AGLYLTGRGDGRLGVEAKLSPRLDTPGIAVRIARDDVAASGHLTPWRVLMLGDTPGAL
                     IESNLIASLNPPTPITDTSWIKPGRTAWDWWSGGVAPDVPDAGMNTATIRRYIDHAAA
                     LKLEYMLIDDGWYVGSTGNGRFNAEADITRAIPQLDLPMLIDHARQRGVGIWVWAHWR
                     SLEPRMDTVFAQYQAMGIKGVKVDFMDRDDQEMVTFFHRLMRSAAAHELMVDMHGAYR
                     PTGLNRTWPNYLTQEGVLGAENNKWSRRITPTHNLTLPFTRMLLGPMDYTPGGFRNVS
                     VDAFEPAFIAPRVMGTRTHQLAMFVIYESPLQMVADSPDAYFDGDGKPAPGTDFLSMV
                     PASWDETRVLSGAIGEHIVIARRRGDDWYIGAMTNEQARTLEVSLAFLGRSGWTATIW
                     ADGDAPTDVLQTQQVLASSDTLRLALSASGGAVVKLSTVKR"
     gene            complement(5132..>5671)
                     /locus_tag="PK399_10765"
     CDS             complement(5132..>5671)
                     /locus_tag="PK399_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPW13642.1"
                     /translation="STHHEGVVAEVLREEPTSLTTWLRELPPGPQCALWLDGVGNPHN
                     LGAILRSAAHFGVGAVLLPKHSTLALSGAAARVAEGGADAVPFVRLGREDNSIAQLHG
                     AGFKLAATVVRGGSDLFKAAMPQRVVYVLGAEAEGMSPDLAKACDLRLSIPGSGAVES
                     LNVASATAVLLSQWASRAR"
BASE COUNT          941 a         1960 c         1881 g          889 t
ORIGIN      
        1 ggccgatctc cggatactgc cggcattgtg tcatggcgcc ccggcgccgc agggaaccga
       61 accacgatgt cgatcttccg caatcccctc ttcacccgct tcaatcgcgt ccgtttcgcc
      121 agctttggcg cgccgcgtcg cccccgccat ccattcctgc gcctcgcact gggactggtc
      181 ggcgtggcgc tgctgatcgc cctgttgttc gtcagcgtat tcgtgggcat cgccatgctg
      241 gcggccggca tgctctttcg cttgttccgg caacgccgcg cgccgatggc cgcgcgcgcg
      301 ggcagcatcg acgccgatta ccgtgtcgtc ggcaaggcgc aactgccgct cgcgcattga
      361 atggatagcc cggcacccgg cgacctcgtc gcagccgcgc cgaccccgcg catccctgtt
      421 cgcgaccgcg gcggcaacct cctcggcacg ttcccggtac ggcgcatcta ctgcgtcggc
      481 cgcaacttcg ccgaccacgc acgcgagatg ggtgccaccg cgccggcatc gaaggccgaa
      541 cgcggcaacc cggtgttctt catgaagccg gccgatgcca tcgtcatcga cggcgtggtg
      601 ccatatccgc cgggcacctc cgacctgcac cacgaggtcg agctggtcgt cgggctgggc
      661 acggacgcgc ctgcaggcga cctgccgctc gacgtcgcag cctcgttggt attcggctac
      721 ggcgtgggcc tggacctgac ccgtcgcgac ctgcaggccg ccgcgaaggc gaaaggcctg
      781 ccctgggaca ccggcaaggg cttcgatcat tccgcgccgg tcagtgacct gctgcccgca
      841 gccgatgccg gcgacatcga gtcgctggca ctgtcgctcg acgtcaacgg cgagcgccgc
      901 cagcagtcca gcctcgacca gctgatctgg aacgtgccgg acatcctgca cgagctctcc
      961 aagctctatg cgcttcgcgc gggagacctg gtgttcatgg gcacgccggc cggcgtggcg
     1021 gcattgcaag ccggcgacac cttcgatgcg cggctgggcg acatcgcgcg attgcagggt
     1081 cacattgcgc aggcagcggt gtaggctcgc cacacgccaa cccgacctga caggagcacg
     1141 cgatgggcat gatgacggag ttcaaggaat tcgcgatgcg cggcaacgtg atcgacctcg
     1201 ccgtcggcgt ggtgatcggc gccgcattcg gcaagatcgt cactgcgctg gtggacaagg
     1261 tcatcatgcc gccgctcggg ctgctgatcg gcggcgtgga tttttcgaag atggcgatcg
     1321 tcctgaagga cgcgaccgtc gatgccgcag gcaaggacgt acccgccgtc gtattggcat
     1381 atggcgagtt catcaatgca ctggtccagt tcgtgatcgt ggccttcgcc atattcctcg
     1441 tggtcaaggc gatcaaccgc atgcacaaga agcccgagga agccccggcc gcgaccccgg
     1501 aagacgtgct gctgctgcgc gagatccgcg attcgctgaa gaagtagccg cgcgacttcc
     1561 agcacatccg acccgaagcc gcgagcctta cactcgcggc ttcgtcgttt ccgggatccg
     1621 ccatgcgtcg caccctgctt gccgctgccc tctcactgct ggtcgcgctg ccgttgcagg
     1681 cacaacagcc cacgcggatc ggcgatgcgg caatgacgca ggccgtcacg ctgcgcgagc
     1741 gcgcattggc cgacgacacc ggctggaagg tcgtcgaatc gctgaccaca gaagtcggcc
     1801 cgcgcctggc cggcagcgag gccgatgcgc gcgcggtgga atgggcgaag gccaagttca
     1861 aggcgctggg ctatgacaaa gtctggaccg agccggtgac cttcccgaag tgggagcgcc
     1921 gcagcgagtc cgcgcgcatc gtcggtgcgc acgcccaagc gctgcacgtg accgcgttgg
     1981 gtggcagccc gggcggcaag gtggaggccg aggtggtgcg tttcgaatcg ctcgaggcgc
     2041 tggagaaggc cgcgcccgga tcgctcgccg gcaagatcgc cttcatcgat taccggatga
     2101 cccgcgcgaa agatggccgc ggctacgggc ccggctcgcg cgtgcgcagc ggcgggccat
     2161 cggcggcgat ccgcgccggc gcgctcgcgt acgtgatgcg ctcggccggc accgactcgc
     2221 atcgcaaccc gcacaccggc atcacccgct tcgatgacgg cctgaagccg atcccctccg
     2281 cggcgttgtc gttgccggac gccgaccagc tggcgcgcct cgtcgcgctg ggccaggtga
     2341 agatcgcgct cgacctggac tgcggctgga acggcgaata cacctcgcag aacgtgatcg
     2401 gcgaaatcac cgggcgctcg aagccggacg acgtggtggt gatcggcgga catctcgatt
     2461 cgtgggacct gggcaccggc gcgatcgatg atggcgcggg cgtcgccatc ggcatggccg
     2521 ccgggcatct gatcggcacg ctgccgaagg ggcagcgacc ggcgcgcacg atccgcgtgg
     2581 tggcgttcgc caacgaggaa cagggcttgt atggcggacg cgcctacgcg cagaagcacg
     2641 ccgccgacct gcgcaagcac cagctcggcg cggaaagcga cttcggtgcg ggccgcatct
     2701 atgccttcgc ggcgaagacg cccgattacg cgaaagaggc cgttgcacgg atcgcgcagg
     2761 cgctggcacc gctcggcatc gagcaccgcg aagacggcga aggctgcggt cccgacctgt
     2821 cgccgttcaa cgcgatcggc atggcctgcg ccggcctgtc gcaggacggc accgactact
     2881 tcgacctgca ccacacgccg gacgacaccc tggacaagat cgacccgaag gcgctggcgc
     2941 agaatgtcgc cgcttacacg gtgttcgcat tcctggccgc cgatgcgcag ggcgatttcg
     3001 gcagcgcgcc gaagccggcc acgccctgac gacccgcatg gcaccagttg ccatgcgaaa
     3061 tcgccatcga cctattccag acgccgcgcg attgacccga taggctccgg gcatccagtc
     3121 cgggggcttg aagatgcaca tccgctgcat tttgtggctg ctgctgtgtg ccttttcgtc
     3181 gttcgccgtt gccggcgaat cgacgccgct gcggctcgat tcgcccggcg gcaacgcgag
     3241 cgtcgaggta tcgctggacc cgcaaggcca accgcactac gccattgcct ggcgcggcat
     3301 gccagtgctg ttgccctcgc cgctcggcgt gcaactgggt ggtgacgacc gcatcgagcg
     3361 cggcctgcgc gtcatcgaca ccacacgcag cagcaccgat tcgagctacg aactcgtcgc
     3421 cggcaagacc cggcatgtac gcgaccacca ccggcaactg gacatcacct tcgccgatgc
     3481 gcaaggacgt cgcctcggcc tgatcgtgcg ggcgtacgac gatggcatcg ccttgcgcta
     3541 tcggctgccg ctgccgccgc agggcgacct ggtggtgaag gaagacctca ccggtttcct
     3601 gttcccgcgg aacctcgcct gccacgcact caacctcggc cgcttcggta ccagccatga
     3661 gggcgagtac gacccgatcc ctgcgtcgct gatccgcccg catcacctga tcgagctgcc
     3721 gctggtctgc ggcaccggca ccggcacgac gacctttgcc atcgccgaag cggacctcga
     3781 tcgctacgcc gggctctacc tcacggggcg tggcgatggc cggctgggcg tggaagcgaa
     3841 actctcgccg cgactggaca cgccgggcat cgccgtgcgc attgctcgcg atgacgtggc
     3901 cgcgagcggc cacctcacgc catggcgcgt gctgatgctc ggcgatacgc ccggcgcgct
     3961 gatcgaatcc aacctgatcg ccagcctcaa tccgcccaca ccgatcaccg acacctcatg
     4021 gatcaagccg ggcaggaccg cgtgggactg gtggtccggc ggcgtggcgc cggacgtgcc
     4081 ggatgccggc atgaacaccg cgacgatcag gcgctacatc gaccatgccg cagcactgaa
     4141 gctggagtac atgctgatcg acgacggctg gtacgtgggc agcaccggca atggtcgctt
     4201 caatgccgaa gccgacatca cccgcgcgat cccgcagctc gacctgccga tgctgatcga
     4261 ccatgcgagg cagcgcggcg tcggcatctg ggtatgggcg cactggcgtt cgctggaacc
     4321 gcggatggac acggtgttcg cgcagtacca agcgatgggg atcaagggcg tcaaggtcga
     4381 cttcatggac cgcgacgacc aggagatggt cacgttcttc catcggttga tgcgcagcgc
     4441 agcggcgcac gaattgatgg tggacatgca cggcgcgtac cggcccaccg ggctcaatcg
     4501 cacctggccg aactacctca cccaggaagg cgtgctgggc gccgagaaca acaagtggag
     4561 ccggcggatc acgcccacgc acaacctcac cctgccattc acccgcatgt tgctgggtcc
     4621 gatggattac acgccgggtg gcttccgcaa cgtgtccgtg gatgccttcg agccagcctt
     4681 cattgcgcct cgcgtgatgg gcacgcgcac ccaccagctg gcgatgttcg tgatctacga
     4741 gagcccgttg cagatggtcg cggacagccc tgacgcctac ttcgatggcg atggcaagcc
     4801 cgccccgggc accgatttcc tgtcgatggt gcccgccagc tgggacgaaa cccgggtgct
     4861 gtccggcgcg atcggcgagc acatcgtcat cgcgcgacgc cgcggcgacg actggtacat
     4921 cggcgcgatg accaacgagc aggcgcgcac gctggaggtg tcgctggcat tcctcggcag
     4981 gagcggatgg acggcgacga tctgggcgga tggcgatgcg ccgacggatg tgctgcaaac
     5041 gcagcaggtg ctggcgtcat cggacacgct caggcttgca ctgtccgcaa gcggcggcgc
     5101 ggtggtgaaa ttgtcaacag taaaacgctg atcagcgcgc gcgactcgcc cactgcgaga
     5161 gcaataccgc cgtggccgag gcgacgttga ggctttccac cgcgccgctg cctggaatcg
     5221 agaggcgcag gtcgcaggct ttcgccaagt cgggcgacat gccttcggcc tccgcgccga
     5281 ggacatagac cacgcgttgc ggcatcgcgg ccttgaacag gtcgctgccg ccgcgcacca
     5341 cggtggcggc cagcttgaaa ccggcgccgt gcaattgcgc gatgctgttg tcctcgcgcc
     5401 ccaggcgcac gaacggcacc gcgtccgcgc cgccttcggc cacgcgcgcc gccgcgcccg
     5461 aaagcgccag cgtggaatgc ttgggcagca gcaccgcgcc cacgccgaaa tgcgcggcgc
     5521 tgcgcaggat cgcgcccagg ttgtgcgggt tgccgacgcc atccagccac aaggcgcatt
     5581 gcgggccggg gggcagttcg cgcaaccacg tggtcagcga cgttggttcc tcgcgcagca
     5641 cttcggcgac cacgccttca tggtgcgtgc t
//
LOCUS       DAOOCX010000143         5623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13574_length_5623_cov_8.666487, whole genome shotgun sequence.
ACCESSION   DAOOCX010000143 DAOOCX010000000
VERSION     DAOOCX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13574_length_5623_cov_8.666487"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PK399_10770"
     CDS             <1..407
                     /locus_tag="PK399_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HPW13643.1"
                     /translation="VVPYIDVMLVLLIIFMVTAPLLNVGVDVNLPQADAKKIADSKKK
                     PVVVQVASDGSLALAYDGVDRPREVSREQMVAMVQGFVSKDPDLAVYVAGDRDVSYQT
                     VYEILSLLQTQAKVPRAGLMGEPVQAAGKPGK"
     gene            418..1296
                     /locus_tag="PK399_10775"
     CDS             418..1296
                     /locus_tag="PK399_10775"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HPW13644.1"
                     /translation="MKESRTDNNVALLQALGLHVALFALMFAGLHWTRSPIAEAAHGD
                     VIEADLVSVSDLSASMQRALRRDPEPLPQPIVEPDPLPPDPEPLPEPVVEPPVEELPP
                     PEPDPAPVEQERVQRDADSAQVAKVDQEQEEKRKKPDQAELDARRRLEALAEIRRQRA
                     LAAQERTMAEQKAQQLNDARNSSATPSRPPPPGNPDSRLAESAGYQVALMNAILRQWT
                     RPESVKLGQVCQVLIRQIPGGEVVSVEISPSCPYDEQGRRSVEAAILKASPLPYAGYE
                     AVFVRNPVLKFRAEDL"
     gene            1391..2698
                     /gene="tolB"
                     /locus_tag="PK399_10780"
     CDS             1391..2698
                     /gene="tolB"
                     /locus_tag="PK399_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HPW13645.1"
                     /translation="MIRIARRLFLSFFLLAPAFSASAQSRGGLEIDIVGGNAAALPIA
                     VVPFGGNAGETDVDDIIRADLARSGQFRTLAEAGLPERPTQGSEVAYPTWRLLKQDFL
                     LVGRSVPDGSGYRVEYELFDVAKQQRLLGFALTAPASAMRDVAHQISDAVYEKILGVP
                     GAFWTRIAYVTSNGLGRNAKYALMVADSDGFNPRTVVNSTEPLMSPSWAPDGRRLAYV
                     SFEGGNSAVYIQDIASGAREKVASFRGINGAPSFSPDGNRLALTLSKGGNPDIYVMDL
                     GSKHLTQVTNHFGIDTEPTWTPDGAGLVFTSDRGGRPQLYQAPAAGGSATRISFEGSY
                     NASPSVSFDGKKIAVAQGAGNTYRIALLDRSLGSPRWSLLSPGSLDESPSFAPNGAMV
                     LYAAREGRRGVLYAVSADGRVRQRLVLAEGDVREPAWSPYRKR"
     gene            2781..3323
                     /gene="pal"
                     /locus_tag="PK399_10785"
     CDS             2781..3323
                     /gene="pal"
                     /locus_tag="PK399_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HPW13646.1"
                     /translation="MNASASRIARTLLLAALASAIAVGCTKKVKEVPPTDTGASTGTT
                     TTPVDDGGATRPGAYGPNDLDTDACLRNRVVYFDLDQDALKPEFQATMACHAKYLRDR
                     PTARLRLEGNADERGSREYNVGLGERRGNAVSSALQANGGSASQQTVVSYGEERPVCN
                     EAGEGCWSQNRRVELVYSAK"
     gene            3323..4114
                     /gene="ybgF"
                     /locus_tag="PK399_10790"
     CDS             3323..4114
                     /gene="ybgF"
                     /locus_tag="PK399_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HPW13647.1"
                     /translation="MRVATTAMVLAAMLASPLALAQRASLADRVAVLEQRAAGDQGAT
                     ELVNQMTQLRSEVQALRGQIEELQQQLEQAKQGQRSQYLDLDGRLNRLEGGVPVASSS
                     SLPPSTAPAQAAPAASAGTATASEPVSGAMAGMDERSAYNHAFEALKSGDYVESSRRF
                     RDFLAVFPGGQYAPNAQYWLGESYYVTQNYALAGDQFQSLLDRFPTHDKAPGALLKLG
                     LSQYGLKQYPAAEATLRQVLQRYPNSDVARTADDRLRAMQLTGAR"
     gene            4124..4813
                     /gene="queE"
                     /locus_tag="PK399_10795"
     CDS             4124..4813
                     /gene="queE"
                     /locus_tag="PK399_10795"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HPW13648.1"
                     /translation="MNAVVDAAAASAAQLQPERLKLTEIFLSLQGEARDAGWPTVFVR
                     LTGCPLRCQYCDTAYAFHGGQWWDIDAILAAVASHGARHVCVTGGEPLAQKRCLVLLA
                     KLCDAGYDVSLETSGAVDIAGVDQRVSRVLDIKTPGSAEVDRNLWGNLPLLTPRDQVK
                     FVICSREDFDWAKAVVAGHRLHEVCDVLFSPSATQLPARELADWIVAEKLPVRFQMQL
                     HKLLWNDEPGR"
     gene            4810..5517
                     /gene="queC"
                     /locus_tag="PK399_10800"
     CDS             4810..5517
                     /gene="queC"
                     /locus_tag="PK399_10800"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPW13649.1"
                     /translation="MTASRKPAVVLVSGGMDSAVVLAIAREAGFDAHALSVRYGQRHT
                     SELDAAARVASALGAVVHKTVHVDLRSIGGSSLTADIAVPTDDDGHEIGVGIPSTYVP
                     ARNTIMLSVALGWSEVLGGTDLFCGVNAVDYSGYPDCRPEFIEGFEQLANLATKAGVE
                     GSRFRVHAPLMRMSKADIVREGLRLGVDFSQTVSCYQADAEGRACRHCDACRLRAQGF
                     DEAGVPDPTRYVDGAIG"
     gene            5552..5623
                     /locus_tag="PK399_10805"
     tRNA            5552..5623
                     /locus_tag="PK399_10805"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5584..5586,aa:Lys,seq:ttt)
BASE COUNT          912 a         1955 c         1881 g          875 t
ORIGIN      
        1 acgtcgttcc ctacatcgac gtgatgctgg tgctgctgat catcttcatg gtcactgcgc
       61 cgctgctcaa cgtcggcgtg gacgtcaacc tgccgcaggc cgatgccaag aagatcgcgg
      121 acagcaagaa gaagccggtc gtcgtgcagg tcgccagcga tggctcgctg gcgttggcgt
      181 acgacggcgt ggatcgcccg cgcgaggtca gccgcgagca gatggtggcg atggtgcagg
      241 ggttcgtgag caaggatccg gacctggccg tgtacgtggc cggcgatcgc gacgtgagct
      301 accagaccgt ctatgaaatc ctcagcctgt tgcagaccca ggccaaggtg ccgcgtgccg
      361 gactgatggg cgagcccgtg caggctgcgg gcaagccggg caagtgacgg ggcaccggtg
      421 aaggaatcgc gcaccgacaa caatgtcgcg ctgctgcagg ccctgggcct gcatgtggcg
      481 ctgttcgcgc tgatgttcgc tggcctgcac tggacgcgca gtccgatcgc ggaagccgcg
      541 catggcgacg tcatcgaagc cgacctggtc agcgtctccg acctcagcgc gtcgatgcag
      601 cgcgccctgc gtcgcgaccc cgagccgttg ccgcagccca tcgtcgaacc cgaccccctc
      661 ccgcccgatc ccgagccatt gccggaaccc gtcgtcgagc caccggtcga ggaactgccg
      721 ccgcccgagc cggatcccgc gccggtcgag caggaacgcg tgcagcgtga tgccgattcc
      781 gcgcaggtcg ccaaggtcga ccaggagcag gaggaaaagc gcaagaagcc ggaccaggcc
      841 gaactggatg cgcgcaggcg gctggaagca ctggcggaaa ttcgtcgcca gcgcgcactg
      901 gcggcccagg aacggaccat ggccgagcag aaggcccagc agttgaacga cgcgcgcaac
      961 agtagcgcca cgccgtcccg gccgccgcca ccagggaatc cggacagccg cctcgcagag
     1021 agcgccggtt accaggtcgc cctgatgaac gcgatccttc ggcagtggac gcgcccggag
     1081 tcggtgaagc tcggccaggt ctgccaggtc ctgatccgcc agatccccgg cggcgaggtg
     1141 gtgtcagtgg agatttcgcc aagctgtccg tacgacgagc agggcaggcg atcggtcgaa
     1201 gcggccatcc tcaaggcctc gccgttgccg tatgcgggct atgaggcggt cttcgttcgc
     1261 aaccccgtgc tcaagttccg cgcagaagac ctctgaatcg ccgaagatgg cggcttggtc
     1321 cgtctccgtt catccagttt cacgccggat gggcgaccct gttcatcttg tcgaccgccc
     1381 gcgaggccgc atgatccgga ttgcccgtcg cctgtttctc tcgttcttcc tgctggcgcc
     1441 tgcattctcg gcatccgcgc aatcgcgcgg cgggctggag atcgacatcg tcgggggcaa
     1501 tgccgcggcc ctgcccatcg ccgtggtgcc gttcggcggc aacgccggcg agaccgatgt
     1561 cgacgacatc atccgcgcgg acctggcccg ctccggccag ttccgcaccc tggctgaggc
     1621 cggcctgccg gaacgcccga cccagggcag cgaggtcgcc tatccgacct ggcgcctgct
     1681 caagcaggac ttcctgctgg tcggccgcag cgtcccggat ggcagcggtt accgggtcga
     1741 gtacgaattg ttcgacgtgg ccaagcagca gcgcctgctc ggtttcgcgc tgaccgcgcc
     1801 ggccagcgcc atgcgcgacg tggcccacca gatcagcgac gcggtgtacg agaagatcct
     1861 cggcgtgccg ggtgcgttct ggacccgcat cgcctacgtg acctcgaacg gcctgggccg
     1921 caacgccaag tacgcattga tggtcgcgga ctccgacggc ttcaatccgc gcacggtggt
     1981 gaattccacc gagccgctga tgtcgccgtc gtgggcaccg gatgggcgcc ggctggccta
     2041 tgtcagcttc gagggcggca actcggcggt gtacatccag gacatcgcca gcggcgcgcg
     2101 cgagaaggtc gccagcttcc gcggcatcaa tggcgcgcct agcttttcac ccgatggcaa
     2161 ccggctggcg ctgaccctgt cgaagggcgg caatcccgac atctacgtga tggacctggg
     2221 cagcaagcac ctgacccagg tgaccaatca cttcggcatc gacaccgagc cgacctggac
     2281 cccggatggc gcgggcctgg tgttcacctc cgatcgtggt ggtcgcccgc agctctacca
     2341 ggccccggcc gccggtggca gcgcgacccg gatcagcttc gagggcagct acaacgccag
     2401 cccgtcggtc agcttcgacg gcaagaagat cgcggtggcg cagggcgcgg gcaacaccta
     2461 ccgcatcgcc ttgctcgacc gcagcctggg cagcccgcgc tggagcctgc tgtcgcccgg
     2521 ttcgctggac gaatccccga gcttcgcccc gaatggcgcg atggtgctgt acgccgcccg
     2581 cgaaggccgc cgcggcgtgc tctacgcggt gtcggccgat ggccgcgtgc gccagcgcct
     2641 ggtgttggcc gaaggcgacg tccgcgagcc cgcctggtcg ccgtatcgca agcgctgacc
     2701 gtcgcgcatc gctcacgctg gcggactaga ctgcaagccc attcaggccc ctgaaccgat
     2761 ttgtcgaacg aggaactccc atgaacgcat ccgcttcccg catcgcccgc accctgctcc
     2821 tcgccgccct ggcctctgcc atcgccgtcg gctgcaccaa gaaggtcaag gaagtcccgc
     2881 cgaccgacac gggcgcgagc accggcacca ccaccacgcc ggtcgatgac ggtggcgcga
     2941 cgcgtccggg cgcctacggc ccgaacgacc tcgataccga tgcctgcctg cgcaaccgcg
     3001 tcgtctactt cgacctcgac caggatgcgc tgaagccgga attccaggcc acgatggcct
     3061 gccacgccaa gtacctgcgc gaccgcccga ccgcgcgcct gcgcctggaa ggcaatgccg
     3121 acgagcgcgg cagccgcgaa tacaacgtgg gcttgggcga gcgccgtggc aacgccgtgt
     3181 cctccgcgct gcaggccaat ggcggctccg caagccagca gaccgtggtc agctacggcg
     3241 aagagcgtcc ggtctgcaac gaggcgggcg agggctgctg gtcgcagaac cgccgcgtcg
     3301 aactggtcta cagcgccaag tgatgcgcgt cgcaaccacg gcgatggtat tggcggcgat
     3361 gcttgcatcg ccgctggcgc tggcccagcg ggcgagcctg gcggatcgcg tggccgtgct
     3421 cgaacagcgc gcggccggcg accagggcgc caccgagctg gtcaaccaga tgacccagct
     3481 gcgcagcgag gtgcaggcgt tgcgcgggca gatcgaggaa ctgcagcagc agctcgaaca
     3541 ggccaagcaa ggccagcgca gccaatacct cgacctcgac ggccggctca accggctgga
     3601 gggcggggtg ccggtagcgt catcgtcttc gttgccgcct tcaactgcac cagcacaggc
     3661 ggctccggcc gcatctgccg gcacggccac ggcgtccgaa cccgtctcgg gcgcgatggc
     3721 cgggatggac gagcgcagcg cctataacca cgcgttcgag gcgttgaagt cgggcgatta
     3781 cgtcgagtcc tcgcggcgct tccgcgattt cctcgcggtg ttccctggcg gacagtacgc
     3841 gcccaacgcg cagtactggc tgggcgagag ttactacgtc acccagaact acgcgctggc
     3901 cggcgaccag ttccagtcgc tgctggatcg cttcccgacc cacgacaagg ccccgggggc
     3961 cctgctcaag ctcggcttgt ctcagtacgg gctgaagcag taccccgccg ccgaggcgac
     4021 cctgcgccag gtcctgcagc gctatccgaa ttccgacgtg gcgcgcaccg cggacgaccg
     4081 cctgcgtgcg atgcaactca ccggcgcccg ttaaactagg cccatgaatg ccgtcgtcga
     4141 tgcggccgcc gcatcggcgg cccagctcca gcccgagcgg ctgaaactca ccgaaatctt
     4201 cctctccctg cagggcgaag cccgcgacgc cggctggccg accgtgttcg tgcgcctgac
     4261 cggctgcccg ctgcgctgcc aatactgcga caccgcttac gccttccacg gcggccagtg
     4321 gtgggacatc gacgcgatcc tggcggcagt cgcctcgcac ggcgcgcgcc acgtctgcgt
     4381 gaccggcggc gagccgctgg cgcagaagcg ctgcctggtc ctgctggcga agctttgcga
     4441 cgccggctac gacgtgtcgc tggaaacttc gggcgcagtc gacattgccg gcgtcgacca
     4501 gcgcgtgtcg cgggtgctgg acatcaagac gccgggttcg gccgaagtcg accgcaacct
     4561 gtggggcaac ctgccgctgc tgacgccgcg cgaccaggtg aagttcgtga tctgctcgcg
     4621 cgaagacttc gattgggcga aggctgtcgt cgccggacat cgcctgcacg aggtctgcga
     4681 cgtgctgttc tcgcccagcg ccacgcagtt gccggcacgc gaactcgcgg actggatcgt
     4741 tgcggaaaaa ctgccggtgc gcttccagat gcaattgcac aaactcctgt ggaacgacga
     4801 gcccggccga tgactgcatc ccgcaaaccc gccgtcgtgc ttgtttccgg tggcatggat
     4861 tccgcggtgg tgctggcgat cgcccgcgag gccggcttcg acgcgcatgc gctcagcgtg
     4921 cgctacggcc agcgccatac gtccgaactc gatgctgccg cgcgcgtcgc gagcgcgctg
     4981 ggtgcggtcg tgcacaagac ggtgcacgtg gacctgcgca gcatcggcgg atcctcgctg
     5041 accgccgaca tcgccgtgcc caccgacgac gacggccacg agatcggcgt cggcatcccg
     5101 tccacctacg tgccggcgcg caacacgatc atgctgtcgg tggcgttggg ctggtccgaa
     5161 gtgttgggcg gtaccgacct gttctgcggc gtgaatgccg tcgactattc cggctatccg
     5221 gactgccggc cggagttcat cgagggcttc gagcagctcg ccaacctggc caccaaggcc
     5281 ggcgtggaag gcagtcgttt ccgcgtgcac gcaccgctga tgcgcatgtc caaggccgat
     5341 atcgtccgcg agggcctgcg cctgggcgtg gatttttcgc agaccgtgag ctgctaccag
     5401 gccgatgccg aaggccgtgc ctgccgccat tgcgatgcct gccgcctgcg tgcgcagggc
     5461 ttcgatgagg ccggcgtgcc cgacccgacg cgctacgtcg acggcgccat cgggtagaat
     5521 gcgcgcccgc gccgatcgcg cggcgcccga tgggtcgtta gctcagtggt agagctgcgg
     5581 acttttaatc cgtaggtcga tggttcgaat ccatcacgac cca
//
LOCUS       DAOOCX010000144         5619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13585_length_5619_cov_9.398454, whole genome shotgun sequence.
ACCESSION   DAOOCX010000144 DAOOCX010000000
VERSION     DAOOCX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13585_length_5619_cov_9.398454"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PK399_10810"
     CDS             <1..969
                     /locus_tag="PK399_10810"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HPW13650.1"
                     /translation="LATRHAGTRKLRIAYELAGPAGAPVVFVAGGISAHRHVAANALD
                     RDAGWAEGLLDAGRALDPANLRILAFDYVGADGSIDAPIDTADQADALALLLDALGID
                     VLEAFVGYSYGALVGLQLAARHPQRLRKLVAVSGVHRAHPYAAAWRALQRKAVALGQL
                     QCADEQGLSLARQFAMLSYRTPEEFGERFDAAPRLCNGHVRVAAEDYLDAAGAKFVAR
                     VSATAWLRLSESIDLHRVDPSSIHVPTTVVAVEGDRLVPMSDAVALVEGLGLNGRLRV
                     LRSQYGHDAFLKETDRIDALLAGELQAGKQRPSPVHDVNDPIGVSV"
     gene            966..2174
                     /locus_tag="PK399_10815"
     CDS             966..2174
                     /locus_tag="PK399_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine (thiol)-lyase"
                     /protein_id="HPW13651.1"
                     /translation="MSQAPTAIDARRATRAVRAGIDRDPAYGAVTPPLVLSSNFSFAG
                     FAEKRQYDYTRSGNPTRDLLGEALAELEGGAGGVVTATGMGAITLALNALLQPGDRLV
                     VPHDCYGGSWRLFNALAKKGAFELITCDLTDPRALAEALQESPALVWIETPSNPLLRI
                     TDLRFVIDAAHKAGALALVDNTFLSPALQRPIEDFDADFVLHSTTKYINGHSDVVGGA
                     VVSKDRAQHDQLSWWANCLGLTGSPFDSFLTLRGLRTLDARLRVHQENTEALVGLLDA
                     HPAVATLYYPGLASHPGHALAARQQHGFGAMLSVELAGGEAAVRAFVDGLQYFTLAES
                     LGGVESLVAHPATMTHAAMSAEARAVAGISDGLLRLSVGIEHADDLLADISAALDRAL
                     SCQDKRKAAP"
     gene            2171..3310
                     /locus_tag="PK399_10820"
     CDS             2171..3310
                     /locus_tag="PK399_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPW13652.1"
                     /translation="MNARIAPLRGSSPKPARVALLGTGVVGSVVWARLASWADTQFGE
                     RLSLVHAANSRFAISNRDGIPAETSDVLMRNPPQASSLDTVEGVLAGQGTRIVIDATA
                     SEQVAARHAHWLARGIHVVTACKLGQGTTLSRWHAIQAAQVAGGTQYGDSATVGAGLP
                     LLRSLRALQAGGDRIHAIAGVLSGSMAWLCNEYDGLRPFSGFVRQAGEAGYTEPDPRD
                     DLSGEDVRRKLLILARASGYPLEADAVQVESLVPEALAGLPRDALGDGLQLLDAPLRE
                     RYAAAYRNGEKLRFVGRLQAGGLATVGLESLPAGHPLVGGAGTDNRVAIWSDRYRERP
                     LVIQGPGAGADVTAAGLLDDALAIQAGEAGAAQVFSGRPKNASIG"
     gene            complement(3268..5457)
                     /locus_tag="PK399_10825"
     CDS             complement(3268..5457)
                     /locus_tag="PK399_10825"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW13653.1"
                     /translation="MQALLASVLRGALLTGLYFAGAVIAVLYLRTPADVTLFWPAAGI
                     GYAAVLRYGLKQAPTIAIAQLLLHLFVVPVPSTFLLYSVASNSVATVLVCAYVQYRRK
                     QLQFRINDGLLLLRGGILLGLISAGIGTLGMLRSGMVPSNELVRVYTQWFLGDLLGVT
                     AVTPSLLFLFNINRRNAAHAMPPEGGRQERVMWALLMFMAMLAAFYIGRQGSMYPLAL
                     ASLPLALLLWSAARYPPIFTACATMGVTMFLALMLGLGVGGFSRPETLQDTSLMMGAL
                     VVHSVIPMLLVASYQERFNAMAALHHRATRDPLTGLLNRDAFEEQARAALSEGTETLT
                     LLYTDLDHFKLVNDSASHVAGDEMIRGVAALVTAEFDDTALIARTGGDEFAVLARLDA
                     AGANARGRRLLAAIEAMRVAWQGQNLGTTASIGLTTSQPPHMAFDELLSKTDAACFEA
                     KELGGNRLFATEQDAEGIQVRTRLMHSAMAAREALDQRRVELWCQPIVDLRSPRPEQA
                     HFEVLMRWRDAEGNLRPPAELIAAAERYRLGPRLDRYVCGEVLAWLEAHPEAAAKVEQ
                     CSINLGAATLTDEDFSDYFANRLRRSPLRPEQFCLEITETSVVRDMTRTRHFISRMRD
                     LGCRFALDDFGTGFCSFGYLRDLDVDYLKIDGSFVRDLDHSGLSEAVVRSITEIAHLL
                     GKRAVGEQAETELQLQHLRALGVDYAQGYVFQRPQPIEAFFGLPEKT"
     gene            complement(5518..>5619)
                     /locus_tag="PK399_10830"
     CDS             complement(5518..>5619)
                     /locus_tag="PK399_10830"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPW13654.1"
                     /translation="DKVIKTMRDTGRDMQDKYKETSRGGLAVNVIEC"
BASE COUNT          842 a         1920 c         1973 g          884 t
ORIGIN      
        1 ctcgccaccc gccacgccgg cacccgcaag ttgcgcatcg cctacgaact ggccggcccc
       61 gcaggcgcgc ctgtggtctt cgtggccggc ggcatttccg cgcatcggca cgtggccgcg
      121 aacgcactgg atcgcgatgc cggctgggcc gaaggcctgc tggatgccgg ccgcgcgctg
      181 gatccggcga acctgcggat cctcgcgttc gactacgtcg gcgccgatgg cagcatcgat
      241 gcgccgatcg acaccgccga ccaggccgac gcgcttgcgc tgctgctgga cgcgctcggc
      301 atcgacgtgc tggaagcctt cgtcggttat tcgtacggcg cgctggtcgg cctgcagctc
      361 gccgcgcggc atccgcaacg cctgcgcaag ctggtcgcgg tcagcggtgt gcatcgcgcg
      421 catccgtatg ccgcggcctg gcgcgcgctg cagcgcaagg cggtcgcgct ggggcaactg
      481 caatgcgccg atgagcaggg tctgtcgctc gcccgccagt tcgcgatgct gagttaccgc
      541 acgccggagg aattcggcga gcgcttcgat gcggcgccga ggctgtgcaa tggccacgtg
      601 cgcgtggccg ccgaggatta cctggacgcg gccggcgcga agttcgtcgc ccgcgtctct
      661 gccaccgcct ggctgcgcct gtccgagtcg atcgacctgc accgcgtgga tccctcgagc
      721 atccatgtgc cgaccaccgt ggtcgccgtc gaaggcgatc ggctggtgcc gatgtccgat
      781 gcggtcgcgc tggtcgaggg gttgggcctg aacggccgcc tgcgcgtgct gcgctcgcag
      841 tacggccacg acgccttcct caaggaaaca gaccgcatcg acgcgctgct ggcaggcgaa
      901 ctgcaggcgg gcaagcaacg tccatccccc gtccacgacg tcaacgatcc gattggagta
      961 tccgtatgag ccaggccccg actgccattg atgcgcgccg cgccacccgc gccgtgcgtg
     1021 ccggcatcga ccgcgacccc gcctacggtg cggtcacccc gccgctggtc ctgtcgtcga
     1081 atttcagctt cgccggcttc gccgagaagc gccagtacga ctacacccgc agcggcaatc
     1141 cgacccgcga cctgctgggc gaggcactgg ccgaactcga gggcggcgcc ggcggcgtgg
     1201 tcaccgcgac gggcatgggc gcgatcaccc tggccctgaa cgcgctgctg cagccgggcg
     1261 accggctggt ggtgccgcat gattgctacg gcggcagctg gcgcctgttc aacgcgctgg
     1321 cgaagaaggg tgcgttcgaa ctgatcacct gcgacctgac cgatccgcgc gcgctggccg
     1381 aggccttgca ggaatcgccg gcgctggtct ggatcgagac gccatccaac ccgctgctgc
     1441 ggatcaccga cctgcgcttc gtgatcgatg ccgcgcacaa ggccggcgcg cttgcgctgg
     1501 tcgacaacac cttcctgtcg ccagcgctgc agcgcccgat cgaggatttc gacgccgact
     1561 tcgtgctgca ttcgaccacc aagtacatca acggccacag cgatgtcgtc ggtggtgcgg
     1621 tggtgtcgaa ggaccgggcg cagcacgacc agctcagctg gtgggcgaac tgcctgggcc
     1681 tgaccggctc gccgttcgac agcttcctca ccctgcgcgg cctgcgcacc ctggatgcgc
     1741 ggctgcgcgt gcaccaggaa aacaccgagg cgctggttgg gttgctggat gcgcatccgg
     1801 cggtcgcgac gctgtactac ccgggcctgg cctcgcaccc ggggcatgcg ctggccgcgc
     1861 gccagcagca cggattcggc gcgatgctga gcgtcgaact ggcaggtggg gaagccgcgg
     1921 tgcgcgcctt cgtcgatggc ctgcagtact tcaccctggc cgagtcgctg ggcggcgtgg
     1981 aaagcctggt cgcgcatccc gccacgatga cccacgcggc gatgagcgcg gaggcgcgcg
     2041 cggtggctgg catctccgac ggcctgctgc gcttgtcggt cggcatcgaa cacgccgacg
     2101 acctgctggc cgatatcagc gcagcgctcg accgcgcgct gtcctgtcag gacaaacgca
     2161 aggccgcgcc gtgaacgcac ggatcgcgcc gctgcgcggg tccagtccca agccggcccg
     2221 cgtggccctg ctcgggaccg gggtcgtggg tagcgtggtc tgggcacggc tggccagttg
     2281 ggccgataca cagttcggcg agcggttgtc gctggtgcat gcggccaatt cgcgcttcgc
     2341 gatcagcaac cgcgacggca tccccgcgga aacgagcgac gtgctgatgc gcaatccgcc
     2401 gcaggcgagt tcgctggata cggtggaagg cgtgctggct ggccagggca cgcgcatcgt
     2461 catcgacgcc accgcctccg aacaggtcgc ggcgcgccat gcgcattggt tggcgcgcgg
     2521 catccacgtg gtcactgcct gcaagctggg gcagggcacc acgttgtcgc gctggcatgc
     2581 aatccaggca gcgcaggtcg cgggcggcac ccagtacggc gacagcgcca cggtcggcgc
     2641 ggggctgccg ctcttgcgca gcctgcgcgc gctgcaggcc ggcggcgacc gcatccacgc
     2701 gatcgccggc gtgctgtcgg ggtcgatggc gtggctttgc aacgaatacg acggcctgcg
     2761 gccgttttcc ggtttcgtcc gccaggccgg cgaggccggt tacaccgagc ccgatccgcg
     2821 cgacgacctc tccggcgaag acgttcgccg caagttgctg atcctggccc gtgccagcgg
     2881 ctatccgctg gaagccgacg ccgtgcaggt ggaatcgctg gtgccggagg cactggctgg
     2941 tttgccgcgc gatgccctgg gcgatggact gcagttgctc gacgcaccgc tgcgcgagcg
     3001 ctacgcggcg gcgtaccgca atggcgagaa gctgcggttc gtcggccgcc tgcaggccgg
     3061 cggcctggcc acggtcgggc tggagtcgct gcccgccggg catccgctgg tgggtggggc
     3121 ggggaccgac aaccgcgtcg cgatctggtc ggaccgctat cgcgagcgcc ccctggtcat
     3181 ccagggtccg ggggcgggcg ccgatgtcac cgccgccggc ttgctggacg atgcgctcgc
     3241 gatccaggcc ggcgaggccg gcgcggctca ggtcttttcc ggcaggccga agaacgcctc
     3301 gatcggctga ggccgctgga agacgtagcc ctgcgcgtag tccacgccca acgcgcgcag
     3361 gtgctgcagt tgcagttcgg tttccgcctg ctcgccgacc gcgcgcttgc ccagcaggtg
     3421 ggcgatttcc gtgatcgaac gcactaccgc ttccgacagt ccggagtgat cgaggtcgcg
     3481 cacgaagctg ccgtcgatct tcaggtagtc gacgtcgagg tcgcgcaggt agccgaacga
     3541 acagaagccg gtgccgaaat cgtccagtgc gaagcggcag ccgagatcgc gcatgcggct
     3601 gatgaagtgc cgcgtgcggg tcatgtcgcg taccacgctg gtctcggtga tctccaggca
     3661 gaactgctcc gggcgtagcg ggctgcggcg cagccggttg gcgaagtagt cgctgaagtc
     3721 ttcgtcggtc agggtcgccg cacccaggtt gatgctgcac tgctcgacct tcgcagccgc
     3781 ctcgggatgc gcttccagcc aggcgaggac ttcgccgcag acataacggt ccaggcgcgg
     3841 acccagccgg tagcgttcgg ccgcggcgat cagttccgcc ggcgggcgca ggttgccctc
     3901 ggcatcgcgc caacgcatca ggacctcgaa atgcgcctgt tccgggcggg ggctgcgcag
     3961 gtcgacgatc ggctggcacc acagctcgac gcgccgctgg tccagcgctt cgcgcgcggc
     4021 catcgcgcta tgcatcaggc gggtgcggac ctggatgccc tcggcgtcct gctcggtcgc
     4081 gaacagccgg ttgccgccga gttccttggc ctcgaagcag gcggcatcgg tcttcgacag
     4141 gagttcgtcg aaggccatgt ggggcggctg gctggtggtc aggccgatgc tggcggtggt
     4201 acccaggttc tgcccttgcc aggccacccg catcgcctcg atcgcggcca gcaggcggcg
     4261 gccgcgtgcg ttcgcaccgg cggcgtcgag ccgcgccagt accgcgaact cgtcgccgcc
     4321 ggtgcgtgcg atcagcgcag tgtcgtcgaa ctcggcggtg accaatgccg ctacgccgcg
     4381 gatcatctcg tcgccggcga catggctggc ggaatcgttg accagcttga aatggtcgag
     4441 gtcggtgtac agcagggtca gggtttcggt gccttcggag agcgcggcgc gcgcctgctc
     4501 ctcgaacgca tcgcggttga gcaggccggt gagcgggtcg cgggtggcgc ggtggtggag
     4561 tgcggccatc gcgttgaagc gctcctggta ggacgcgacc agcagcatcg ggatcaccga
     4621 atgcaccacc agcgcgccca tcatcagcga ggtgtcctgc agcgtctccg gccgggagaa
     4681 gccacccacc ccgagcccca gcatcagcgc caggaacatg gtcacgccca tcgtcgcgca
     4741 ggcggtgaag atcggtggat accgcgctgc cgaccagagc agcagggcca gcggcaggct
     4801 ggccagcgcc agcgggtaca tgctgccctg gcgaccgatg tagaacgcag ccagcatcgc
     4861 catgaacatc agcagcgccc acatcacccg ctcctgccgc ccgccttccg gtggcatggc
     4921 atgggccgcg ttgcgacggt tgatgttgaa caggaacagc aggctggggg tgaccgcggt
     4981 gacgccgagc aggtcaccga ggaaccattg ggtatagacc cgcacgagct cgttggacgg
     5041 gaccatcccg gaccgcagca tgccgagcgt gccgatcccg gcgctgatca gtcccagcag
     5101 gatgccgccg cgcagcagca gcagcccatc gttgatgcgg aattgcagct gcttgcggcg
     5161 gtactgcacg taggcgcaga cgagcaccgt cgccaccgag ttgctggcca ccgaatacag
     5221 caggaaggtg cttggcaccg gtaccacgaa caggtgcagc agcagttgcg cgatggcgat
     5281 cgtcggcgcc tgcttgaggc catagcgcaa caccgcggcg tagccgatgc cggcagcggg
     5341 ccagaacagg gtgacgtccg ccggggtgcg caggtaaagc accgcgatca ccgccccggc
     5401 gaagtacagc ccggtcagca gtgctccccg taacacgctc gccagcaagg cttgcatgca
     5461 ttcagtctag accgggaatg ccccgcggtg tcaccactct cgcgtcaggt tgcgatctca
     5521 gcactcgatc acgttgaccg ccagcccgcc ccggctggtt tccttgtact tgtcctgcat
     5581 gtcgcggccg gtgtcgcgca tggtcttgat gaccttgtc
//
LOCUS       DAOOCX010000145         5613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13596_length_5613_cov_8.093919, whole genome shotgun sequence.
ACCESSION   DAOOCX010000145 DAOOCX010000000
VERSION     DAOOCX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13596_length_5613_cov_8.093919"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PK399_10835"
     CDS             <1..307
                     /locus_tag="PK399_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13655.1"
                     /translation="LEVTFDYWVRIAAEVRATGARKLLVVDEMQGEVMTDADLERFFA
                     GMSGRGLEGLRIAYVEGRPDQMQRIEFVEQMALERGYTIRVFGNERDAVFWLRHGLR"
     gene            complement(332..1141)
                     /locus_tag="PK399_10840"
     CDS             complement(332..1141)
                     /locus_tag="PK399_10840"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007966679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HPW13656.1"
                     /translation="MASPFGTETVLDVRHWTDAYFSFTTTRDDGFRFDNGQFVMIGLE
                     VEQPDGSRKPLMRAYSIASANWEEQLEFFSIKVQDGPLTSRLQHIQPGDSILIGRKPT
                     GTLLVSDLHAGRNLYLLGTGTGFAPWLSVIKDPETYERFERVVLCHGVRGAEDLAYRD
                     YIERELPNHELLGDTLRDRLLYYPAVSREPFTYNGRDHQGRITDHLDQGRIATALGLE
                     DLDPARDRAMICGSPQMLADFRGILDRRGFTASPRIGTAGEYVFERAFVEK"
     gene            complement(1222..2397)
                     /locus_tag="PK399_10845"
     CDS             complement(1222..2397)
                     /locus_tag="PK399_10845"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPW13657.1"
                     /translation="MRRLRLLSTCIAMSLALNACAQSQAPAQPATSAEPARSMPTQAG
                     TLQVRPIASGLEHPWAVALLPGGDFLVTERPGRLRRIAANGTVSPPITGVPTVFAEGQ
                     GGLLDVVLDPDFASNKRIWLSFAEPGDGDTAGTAVATATLGDAALTDVGVIYRQLPKL
                     EGGNHFGSRIAFDGKGHVFISQGERNERAMAQDLEVLQGKLVRLNVDGTQPADNPFAS
                     GVGVRKAIWSYGHRNMQGLAVDPRTGTLWESEHGPRGGDELNLPRAGKNYGWPVITHG
                     INYSGLKIPEAEGKAKAGMEQPHHVWEKSPGLSGMAFFVGRPRHPWNNSLLLGALADR
                     NLIRLTLDGDKVVSEERLLGDLGKRIRDVRVSADGKVYVLTDEDDGQLLELVPPAAP"
     gene            complement(2411..4264)
                     /locus_tag="PK399_10850"
     CDS             complement(2411..4264)
                     /locus_tag="PK399_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW13658.1"
                     /translation="MPPPLPAAQLNLRQRFAAMRNIAPFLREIWATSKPLTTASIGLR
                     LIRAFLPIATLYVGKLIIDEAIRLIGLGVGQELAAAWHSGALDHLLALLGLEFGLAIG
                     SDLLGRLTSYVDTLLSERFTNATSVRLMEHAATLDLEDFEDAELQDKLDRARRQTMGR
                     MNLMGQLFGQAQDAITVVSFAIGLLAYAPWLMLLLAVALIPAFVGESHFNALNYSLNF
                     QWTPERRQLEYLRQVGASVETAKEVKIFNLNRFFIERFRTLSQKFYEANARLAGKRAF
                     WGVLLAALGTLGYYVAYAYIAWRTVRGDFSIGDLTFLAGSFRRLRQLLESLLVGFSQV
                     AGQALYLDDLYSFFEIEPEIRSKPDAIAVPDPIRQGFVFENVGFRYEGAERWALRGLS
                     FELHAGEVLALVGENGAGKTTLVKLLARLYDPDEGRILLDGRDLRDYDLDDLRANTGV
                     IFQDFVRYHLTAAENIGVGLIEAMGDRARVELAARKGMADEVVASLPKGYDQIIGRRF
                     KEGVDLSGGQWQKIAIARAYMRDAQVMILDEPTAALDARSEFEVFERFKELSDGKTAV
                     LISHRFSSVRMADRILVLADGKVESSGTHEQLMAQGGRYAELFELQAAGYR"
     gene            complement(4415..4912)
                     /locus_tag="PK399_10855"
     CDS             complement(4415..4912)
                     /locus_tag="PK399_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HPW13659.1"
                     /translation="MSGNDEVSRRFEGIRGILNDLRLMLGDMLAMGRPSADQQMVIEV
                     FFGSMGYLAKADRLVTSHESDLANRFMDELDLSMAARRIASEAFERGMHRNINLQGEL
                     LRFTDAHPPGSEDSKRLYDILVRLAASDSKLDQREYDVMAQVTKALGLPPETLYARLP
                     APAPR"
     gene            5024..>5613
                     /locus_tag="PK399_10860"
     CDS             5024..>5613
                     /locus_tag="PK399_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPW13660.1"
                     /translation="MTAIKQEDLVQSVADALQYISYYHPVDYIRSLAAAYEREESPAA
                     KDAMAQILINSRMCAEGRRPICQDTGIVTVFLEVGMDVRWDGFTGSLEDAVNEGVRRA
                     YHHPDNKLRASVLADPAGKRTNTRDNTPAVVNMKIVPGDKLDVIVAAKGGGSEAKSKF
                     AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGT"
BASE COUNT          909 a         1831 c         1944 g          929 t
ORIGIN      
        1 gctggaagtc accttcgatt actgggtgcg gatcgctgca gaagtgcgcg cgaccggtgc
       61 ccgcaagttg ctggtagtcg acgagatgca aggcgaggtc atgaccgacg cggacctcga
      121 gcggttcttc gccggcatga gcgggcgcgg gttggaaggc ctgcgcatcg cctacgtcga
      181 gggccgcccg gaccagatgc agcgcatcga attcgtcgag cagatggcgc tggagcgtgg
      241 ctacacgatc cgggtgttcg gcaacgagcg cgacgcggtg ttctggctgc gccacggctt
      301 gcggtaagcg ccggccacgc cggcgcgcgc ctcacttctc gacgaaggcg cgctcgaaca
      361 cgtactcgcc cgcggtgccg atgcggggcg aggcggtgaa gccccggcga tcgaggatgc
      421 cgcggaaatc cgccagcatc tgcgggctcc cgcagatcat cgcgcggtcg cgcgcggggt
      481 cgaggtcttc gaggcccagg gccgtcgcga tgcggccctg gtcgaggtgg tcggtgatgc
      541 ggccctggtg gtcgcggccg ttgtacgtga acggttcgcg cgagaccgcc gggtagtaca
      601 gcaggcggtc gcgcagggtg tcgccgagca gttcgtggtt cggcaattcg cgttcgatgt
      661 agtcgcggta ggccaggtcc tcggcgccgc gcacgccgtg gcagagcacc acgcgttcga
      721 agcgttcgta agtctccgga tccttgatca ccgacagcca cggcgcgaag ccggtaccgg
      781 tacccagcag gtagaggttg cggcctgcgt gcaggtcgga gaccagcagg gtgcccgtgg
      841 gcttgcggcc gatcaggatc gaatcgccgg gctggatgtg ctggaggcgc gaggtcagcg
      901 ggccgtcctg caccttgatg ctgaagaact cgagttgttc ttcccagttc gcgctggcga
      961 tggagtacgc gcgcatcagc ggcttgcggc tgccgtccgg ctgctcgacc tccagcccga
     1021 tcatgacgaa ctggccgtta tcgaaacgga agccgtcgtc gcgggtggtg gtgaagctga
     1081 agtacgcgtc cgtccagtga cgaacgtcga gcaccgtctc ggtgccgaac ggggaggcca
     1141 tgcggggatc tggggaaagc ggggcaggcc gccattttac gctgcgccgc agcgaggcgt
     1201 ttgacctcgc gcaaacgggg ttcagggcgc ggcgggcggc accagctcca gcaactggcc
     1261 atcgtcctcg tcggtgagca cgtagacctt gccatccgcg gagacccgca catcacggat
     1321 gcgcttgccc aggtcgccga gcaggcgctc ttcggagacg accttgtcgc cgtccagggt
     1381 cagccggatc aggttgcgat ccgccagcgc gcccagcagc aggctgttgt tccaggggtg
     1441 cctggggcgg ccgacgaaga acgccatgcc ggacaggccc ggcgacttct cccagacgtg
     1501 atgcggctgt tccatgccgg ccttcgcctt gccttcggct tcggggatct tcaggccgga
     1561 atagttgatg ccgtgggtga tgaccggcca gccgtagttc ttgccggccc ggggcaggtt
     1621 gagttcgtcg ccgccgcgcg ggccgtgctc gctttcccac aacgtaccgg tgcgcggatc
     1681 gaccgccagt ccctgcatgt tgcgatggcc gtagctccag attgccttgc gcacgcccac
     1741 gccactggcg aacggattgt ccgcaggctg ggtgccgtcg acgttcagcc gcaccagctt
     1801 gccctgcagg acttccaggt cctgcgccat ggcgcgctcg ttgcgttcgc cctggctgat
     1861 gaagacatga cccttgccgt cgaaggcgat gcgcgagccg aaatggttgc cgccttccag
     1921 cttcggcaat tgccggtaga tcacgccgac gtccgtcaat gcggcatcac ccaaggtggc
     1981 ggtggcgacc gcggtgccgg cggtatcgcc gtcgccgggt tcggcgaagc tcagccagat
     2041 gcgcttgttg ctggcgaaat ccggatcaag caccacatcg agcaagccgc cctgtccctc
     2101 ggcgaacaca gtggggacac cggtgatcgg tggcgaaacc gtgccgttcg cggcgatccg
     2161 gcgcaaacgg ccgggacgtt cggtgaccag gaagtcgccg ccggggagca atgcgacggc
     2221 ccacggatgt tcaagcccgg acgcgatcgg ccgaacctgc aacgtgccgg cctgggtcgg
     2281 catcgaccgc gcgggttctg ccgaggtggc cggctgggca ggcgcctgcg attgcgcaca
     2341 ggcattcaac gccaacgaca tcgcgatgca ggtggaaagg aggcgaagtc ggcgcattcg
     2401 ggttccttgt tcagcgatag cctgcggcct gcagttcgaa gagttcggca tagcgtcccc
     2461 cctgcgccat caattgttcg tgagtcccgc tggattcgac cttgccgtcc gccagcacca
     2521 ggatacggtc ggccatgcgc acgctgctga agcggtggct gatcagcact gcggtcttgc
     2581 cgtcggacag ttccttgaag cgctcgaaca cctcgaactc gctgcgcgcg tcgagcgcag
     2641 cagtgggttc gtcgaggatc atcacctgcg catcgcgcat gtaggcgcgg gcgatggcga
     2701 tcttctgcca ctgtccgccg gacaggtcca cgccctcctt gaagcggcgg ccgatgatct
     2761 ggtcgtagcc cttcggcagc gaggccacca cctcgtcggc catgcccttg cgcgcggcca
     2821 gctcgacccg cgcgcggtcg cccatcgcct cgatcaggcc cacgccgatg ttctccgcgg
     2881 cggtgaggtg gtagcgcacg aagtcctgga agatcacgcc ggtattggcg cgcaggtcgt
     2941 ccaggtcgta gtcgcgcagg tcgcggccgt ccagcaggat gcggccttcg tcggggtcgt
     3001 acaggcgcgc cagcagtttc accagggtgg tcttgccggc gccgttctcg cccaccaggg
     3061 ccagcacttc gccggcatgc agttcgaagc tcaggccgcg cagcgcccag cgctccgcgc
     3121 cttcgtagcg gaagcccacg ttctcgaaca cgaagccctg gcggatcggg tcgggcaccg
     3181 cgatcgcatc cggcttgctg cggatctccg gttcgatctc gaagaacgaa tacaggtcgt
     3241 ccaggtacag cgcctgcccg gccacctgcg agaacccgac cagcaggctt tcgagcaact
     3301 ggcgcaggcg ccggaagctg ccggccagga aggtcaggtc accgatgctg aagtcgccgc
     3361 gcaccgtgcg ccaggcgatg taggcatagg ccacgtagta gccgagcgtg cccagcgcgg
     3421 ccagcagcac gccccagaac gcacgcttgc cggccagcct ggcgttggcc tcgtagaact
     3481 tctgcgacag cgtgcggaag cgctcgatga agaagcggtt gaggttgaag atcttgacct
     3541 ctttcgcggt ctccacgctg gccccgacct ggcgcaggta ttccagctgg cggcgttccg
     3601 gcgtccactg gaagttgagc gagtagttga gcgcattgaa atgcgattcg ccgacgaagg
     3661 cggggatcag cgccacggcc agcagcagca tcagccacgg tgcgtaggcc agcagtccga
     3721 tggcgaagct gaccacggtg atcgcatcct gcgcctggcc gaacagctgg cccatcaggt
     3781 tcatccggcc catggtctgc cggcgcgcac gatccagctt gtcctgcagc tcggcgtcct
     3841 cgaaatcctc caggtccagg gtggccgcat gttccatcag gcgcacgctg gtggcgttgg
     3901 tgaagcgctc ggacagcagg gtgtcgacgt agctggtcag ccggcccagc aggtcggagc
     3961 cgatcgccag cccgaattcc agccccagca gggccagcag gtggtcgagc gcgcccgaat
     4021 gccaggccgc cgccagttcc tggcccacgc ccaggccgat caggcggatc gcttcgtcga
     4081 tgatcagctt gccgacgtag agggtggcga tcggcaggaa ggcgcggatc aaccgcaggc
     4141 cgatcgaggc cgtggtcagt ggcttgctgg tcgcccagat ctcgcgcagg aagggcgcga
     4201 tgttgcgcat cgcggcgaag cgctggcgca ggttgagttg ggcggcgggc aggggaggcg
     4261 gcatgcgccg attgtgcggg aaacgcccgt gcagggcgtt tcacgatggg catcgacctg
     4321 tttcagcgat gccgttgcca agcggcatcg ccacagtgag cgttggctgg aacacaggtc
     4381 ccgcggaact cgcgggacgg gtcatggggc gtgatcagcg cggtgctggc gccggcagtc
     4441 gtgcgtacag cgtttccggc ggcaggccga gcgccttggt gacctgcgcc atgacgtcgt
     4501 attcgcgctg gtcgagcttc gagtccgagg ccgccagccg caccaggatg tcgtagaggc
     4561 gcttggaatc ctccgagccg ggcgggtgcg catcggtgaa gcgcagcagc tcgccctgca
     4621 ggttgatgtt gcggtgcatg ccgcgctcga acgcttccga ggcgatccgg cgcgccgcca
     4681 tcgacaggtc gagctcgtcc atgaagcggt tggccaggtc ggattcgtgg ctggtgacca
     4741 ggcggtcggc cttggccagg tagcccatcg agccgaagaa cacttcgatg accatctgct
     4801 ggtcggcgct tggccgcccc atcgccagca tgtcgcccag catcaggcgc aggtcgttga
     4861 ggatgccgcg gataccctcg aagcgcctgg aaacctcgtc gttgccgctc atcgtgtccc
     4921 cctccagaac attggccgag cccccatcct gcgccacccg cggcctgctg aaaagggcgg
     4981 cgcgatatca ttcgcctccc cccgcgtcca gccagccaga tccgtgaccg ccatcaagca
     5041 ggaagacctc gtccagtccg tcgccgacgc cctccagtac atcagctact accatccggt
     5101 ggactacatc cgcagcctgg ccgccgccta cgagcgcgag gaatcgcccg cggccaagga
     5161 cgcgatggcg cagatcctga tcaattcgcg gatgtgcgcg gaagggcgcc ggccgatctg
     5221 ccaggacacc ggcatcgtca ccgtgttcct ggaggtgggc atggatgtgc gctgggacgg
     5281 cttcaccggt tcgctggagg acgccgtcaa cgagggcgtc cgccgcgcct accaccaccc
     5341 cgacaacaag ctgcgcgcca gcgtgctggc cgatccggcc ggcaagcgca cgaacacccg
     5401 cgacaacacg ccggccgtcg tcaacatgaa gatcgtgccg ggcgacaagc tggacgtgat
     5461 cgtggccgcc aagggcggtg gttccgaggc caagtccaag ttcgccatgc tcaacccgtc
     5521 cgattcgatc gtcgactggg tgctgaagac ggtgccgacg atgggcgccg gctggtgccc
     5581 gccgggcatg ctcggcatcg gcatcggcgg cac
//
LOCUS       DAOOCX010000146         5604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13619_length_5604_cov_8.057848, whole genome shotgun sequence.
ACCESSION   DAOOCX010000146 DAOOCX010000000
VERSION     DAOOCX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13619_length_5604_cov_8.057848"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PK399_10865"
     CDS             <1..549
                     /locus_tag="PK399_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424120.1"
                     /note="ATPase; with MinC inhibits cell division by
                     blocking formation of the polar Z ring septums; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HPW13661.1"
                     /translation="SQTRDKDALTKEGVEKVLKDLADDGFDYIVCDSPAGIEKGAFLA
                     MYFADKAVVVVNPEVSSVRDSDRILGLLASKTRRAETGEGEVTAYLLLTRYSPQRVEN
                     GEMLSITDVEEVLGLKTIGVVPESGDVLNASNKGEPVIMDAESNAGQAYDDAVARLLG
                     EQRPMRFTQLEKKGFFSKLFGG"
     gene            553..813
                     /gene="minE"
                     /locus_tag="PK399_10870"
     CDS             553..813
                     /gene="minE"
                     /locus_tag="PK399_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HPW13662.1"
                     /translation="MSIFDFLKPKKTTAITAKNRLQIIIAQERSSQGAPDYLPLMRRE
                     ILEVIRKYVNVDVDAVKVDVVKDGDHDVLDISVSLPDGRASA"
     gene            910..1410
                     /locus_tag="PK399_10875"
     CDS             910..1410
                     /locus_tag="PK399_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13663.1"
                     /translation="MVEAASVLTLADIHFDDAAALLHRFGLSLQRVADGAPIPGSYWG
                     ECEAGIIGNAVHARSDTPVHSLLHEAGHLIVLPPERREAVHTDATDSIPEEDAVCVLQ
                     GLLGDALPGVGRDRVLADMDRWGYTFRLGSARAYVDHDADDAWAWLQARGLVDAARTL
                     LLPPIR"
     gene            1544..3535
                     /locus_tag="PK399_10880"
     CDS             1544..3535
                     /locus_tag="PK399_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014089618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HPW13664.1"
                     /translation="MKPRIALLALAIVGSLAITACKRTPTPPSPAPGAPAAAPAGETA
                     DQFVARVNEEFRAISAELNAAQWLSNTYINGDSEMLAAKANERWLTQLNAWIAQAKRF
                     EGQQLSPDTARAIKLIKLMTAMPAPKDPKLLQELTRLATRMEGTYGAGKYCTGTGEAT
                     TCRDIGQLSEVLAKSRDYDAQLDAWQGWHTISQPMRKDYIRFAELVNTGAREMGFADA
                     GEMWRSGYDMSPAELAAESDRLWDQVKPLYEQLHCYARGKLDATYGKDKGEVAGGMLP
                     AHLMGNLWQQDWGNLWDLMQPYPGAGSLDVNSALQKLADDKLGIELDKPGDRNASEAE
                     RTFAAQRAAELATAKEMTVRAQDFYVSLGMPKLPDSYWENSQFIKPLDREVVCHASAW
                     DMNMAGDVRTKMCIKPNEEELTTIYHELGHIYYDLAYNPQPPVFQGGAHDGFHEAIGD
                     TIVLAMTPAYLQSVGLVGAQQRSQEALVNAQMRMALAKVSFLPFGLMIDRWRWGVFDG
                     SIKPDSYNKAWWDLKAKYQGVAPITPRGEDFFDPGAKYHVPANTPYTRYFLSHVLQFQ
                     FYKALCDASGYQGPLYECSFYGSKAAGEKYSAMLSKGASQPWQKTMKELTGGEKMDAS
                     AVLEYFAPLQAWLKQQNAGSACGWNPSGGAAATASTKAA"
     gene            3686..3997
                     /locus_tag="PK399_10885"
     CDS             3686..3997
                     /locus_tag="PK399_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPW13665.1"
                     /translation="MQRIPKLAIASLLLASSLPFVAHAQSSGGNIAGEASVGDTVTIT
                     GVDTGFRRELKIDKDGKYQVRRVPTGDYMVVLVHKDGSIDPAQAVTVRVGSTARVMAP
                     K"
     gene            4099..5178
                     /locus_tag="PK399_10890"
     CDS             4099..5178
                     /locus_tag="PK399_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPW13666.1"
                     /translation="MHTPVELAPQHPATATALPAHYYVDASMAARDRASIFDAAWQLI
                     AHVSQLRNPGDHVVADFAGLPVLAVRGADDAIRVFHNVCRHRAGPIAQCDGLAAKSLR
                     CRYHGWTYTLEGQLRAAPEMNGVEAFDVADIRLPQLAVTVWQGLVFASVDATRAPGFD
                     AFVAGIDARLGADRGLERYGNHHRASYDIACNWKVYVDNYLEGYHVPHVHPGLNRLLD
                     YRSYRTELERWHSLQWSPLESDATLYGNGDALYYWLWPNTMLNILPGRMQTNTVIPLG
                     VDRCRVLFDSYYAPDDSDAGKARREADLAFSDEVQHEDLGICEDVQRGFASGSYVPGR
                     LNPLRESGVHHFHEMLRAAYRTSAI"
     gene            5322..>5604
                     /locus_tag="PK399_10895"
     CDS             5322..>5604
                     /locus_tag="PK399_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPW13667.1"
                     /translation="MATALVVGSMIGSGVFLLPAALAPYGAASLVGWAIALCGALLLA
                     ATFAKLAVHWPCTGGPYAYVRRSFGDVAGFGITWSYWISIWSGMAAIAVA"
BASE COUNT          974 a         1940 c         1813 g          877 t
ORIGIN      
        1 tcgcagaccc gcgacaagga cgcgctgacc aaggaaggcg tggagaaggt gctcaaggac
       61 ctggccgacg acggcttcga ctacatcgtc tgcgactcgc ccgccggcat cgagaaaggg
      121 gcgttcctgg cgatgtactt cgccgacaag gcggtggtgg tggtgaaccc ggaagtctcc
      181 tcggtccgcg actccgaccg catcctgggc ctgctggcca gcaagacccg ccgcgccgag
      241 accggcgagg gcgaggtcac cgcctacctg ctgctcaccc gctacagtcc gcagcgcgtc
      301 gagaacggcg agatgctgtc gatcaccgac gtcgaggaag tactgggcct gaagacgatc
      361 ggcgtggtcc cggaatccgg cgacgtgctc aacgcctcca acaagggcga gccggtgatc
      421 atggatgccg aatccaacgc cggccaggcc tacgacgacg ccgtcgcgcg cctgctcggc
      481 gaacagcgcc cgatgcgctt cacccagctg gagaagaagg gcttcttcag caagttgttc
      541 gggggctgac gcatgtcgat cttcgatttc ctcaagccca agaagaccac tgcgatcacc
      601 gcgaagaatc gcctgcagat catcatcgcg caggagcgca gctcgcaagg cgcgccggat
      661 tacctgccgc tgatgcgccg ggagatcctg gaagtgatcc gcaagtacgt gaacgtggat
      721 gtcgacgcag tcaaggtcga cgtggtcaag gacggcgacc acgacgtgct cgacatctcg
      781 gtgtcgctgc cggacgggcg cgcaagcgcc tgacgggcca tccgtcgtcc cggcgaacgc
      841 cgggatccag catttcatct cgccatgcag gacgaaccgg acttcggcgt tcgccgggag
      901 gatgcgtcga tggtggaggc cgcgtccgtc ctcaccctcg ccgacatcca tttcgacgat
      961 gcagccgcct tgctgcatcg cttcggattg tcgttgcagc gcgtcgccga cggcgcaccg
     1021 attcccggca gctactgggg cgagtgcgag gccgggatca tcggcaacgc cgtccatgcc
     1081 cgcagcgaca cgccggtgca ttcgctgttg catgaagccg gccacctgat cgtgttgccg
     1141 ccggaacgcc gcgaagcggt acacaccgac gccaccgatt cgattcccga ggaagacgct
     1201 gtctgcgtgc tgcagggcct gctgggcgat gccttgccgg gtgtcggccg cgaccgcgtg
     1261 ctcgccgaca tggaccggtg gggctacacc ttccgcctgg gttccgcgcg cgcctatgtc
     1321 gaccacgatg ccgacgatgc gtgggcgtgg ctgcaagcac gcggactggt cgacgcagcg
     1381 cgcaccctgc tcctcccgcc tatccgctag cgaagcgcgt ggcgggtagc gctgcgccgg
     1441 gcctttcggg ccgaggcagt gcttccggcc gcatgcttgc cgagcccgca agtgcggtct
     1501 agccttgccc ctccagcgcg ccagcgccgc cgaggaacgc accgtgaagc cacgcatcgc
     1561 cctgctcgcc cttgccatcg tcggctcact cgcgatcacc gcctgcaagc gcacgcccac
     1621 tccacccagc ccggcacccg gggcgcctgc cgcagccccc gccggcgaaa ccgccgacca
     1681 gttcgtcgcc cgggtcaacg aggagttccg cgcgatcagc gcagaactga acgcggcgca
     1741 atggctgtcg aatacctaca tcaatggcga cagcgaaatg ctggcggcca aggccaacga
     1801 acgctggttg acccaactca acgcttggat cgcgcaggcg aagcgcttcg aaggccagca
     1861 gctttcgccg gacaccgcgc gcgcgatcaa gctgatcaag ctgatgaccg cgatgccggc
     1921 gccgaaggac ccgaagctgc tgcaggagct gacccggctc gccacccgca tggaaggcac
     1981 ctatggcgcg ggcaagtact gcaccggcac cggcgaggcg accacctgcc gcgacatcgg
     2041 ccaactcagc gaggtgctcg cgaagagccg cgactacgac gcccagctgg acgcctggca
     2101 gggctggcac accatcagcc agccgatgcg caaggattac atccgctttg ccgaactggt
     2161 gaacaccggc gccagggaaa tgggcttcgc cgatgccggc gagatgtggc gctcgggcta
     2221 cgacatgagc ccggccgaac tcgccgccga gagcgatcgc ctgtgggacc aggtcaagcc
     2281 cctgtacgaa cagctgcact gctatgcgcg cggcaagctg gatgcgacct acggcaagga
     2341 caagggcgag gtggccggcg gcatgttgcc cgcgcacctg atgggcaacc tgtggcagca
     2401 ggactggggc aacctgtggg acctgatgca gccctacccg ggtgccggca gcctggacgt
     2461 caactccgcg ctgcagaaac tcgccgacga caagctgggc atcgagctcg acaagcccgg
     2521 cgaccgcaac gccagcgaag ccgagcggac gttcgccgcc cagcgtgccg cggagcttgc
     2581 caccgccaag gaaatgaccg tgcgcgcgca ggacttctac gtctcgctgg gcatgccgaa
     2641 gttgccggac agctactggg agaactcgca gttcatcaag ccgctcgatc gcgaggtggt
     2701 ctgccacgcg agcgcctggg acatgaacat ggccggcgac gtgcgcacca agatgtgcat
     2761 caagccgaac gaggaagagc tcaccaccat ctaccacgag cttggccaca tctattacga
     2821 cctggcctac aacccgcaac cgccggtgtt ccagggtggc gcgcacgacg gcttccacga
     2881 agcgatcggc gacaccatcg tgctggcgat gacgccggcc tacctgcagt cggtcggcct
     2941 ggtcggcgcg cagcagcgga gccaggaagc gctggtcaat gcgcagatgc gcatggcgct
     3001 ggccaaggtg tcgttcctgc cgttcgggct gatgatcgac cgctggcgct ggggcgtgtt
     3061 cgacggctcg atcaagcccg acagctacaa caaggcgtgg tgggatctga aggcgaaata
     3121 ccagggcgtc gcgccgatca caccacgcgg cgaggacttc ttcgaccccg gcgcgaagta
     3181 ccacgtgccg gccaacaccc cctacacgcg ctacttcctt tcgcacgtgc tgcagttcca
     3241 gttctacaag gccctgtgcg atgcctccgg ctaccagggt ccgctgtacg aatgcagctt
     3301 ctacggcagc aaggccgccg gcgagaagta ttcggcgatg ctgtccaagg gcgcgagcca
     3361 gccgtggcag aagaccatga aggaactcac cggcggcgag aagatggatg ccagtgcagt
     3421 gcttgaatac ttcgcgccat tgcaggcctg gctgaagcag cagaacgccg gtagtgcctg
     3481 cggctggaat ccgtccggcg gtgccgcggc gacggcctcc accaaggcgg cctgacgacg
     3541 accgcgcttg caacacgaag gccggcagca tgccggcctt cgtcttcatg gcagagcgcg
     3601 cacgccttgc atgccgaaag tcacttgtcc ggcaaccggg acttcatgca gtttcccggt
     3661 tccctccacg ccgtccggtc ccgccatgca gcgcatcccc aagctcgcta tcgccagcct
     3721 gctcctcgcc agttccctgc ccttcgtggc ccatgcccaa tccagcggcg gcaacatcgc
     3781 cggcgaggcc tccgttggcg ataccgtgac catcaccggc gtcgacaccg gcttcaggcg
     3841 cgaactgaag atcgacaagg acggcaagta ccaggtgcgt cgcgtgccga ccggcgacta
     3901 catggtcgtg ctggtccaca aggacggctc gatcgatcct gcccaggccg tcacggtgcg
     3961 cgtgggcagc acggcacgcg tgatggcccc gaaatagccc ttggccaacc cggcctcgcg
     4021 cagcacagtg ccgatggacg tgaggccagc agctgctggc ctcctgtttt ttcgtcgcca
     4081 cgggcggata ctcccccgat gcacaccccc gtcgaactcg ccccgcaaca ccccgccact
     4141 gccaccgcgc tgccggcgca ctactacgtc gacgcctcga tggccgcgcg cgaccgcgcg
     4201 tcgatcttcg atgcagcgtg gcaactgatc gcgcatgtgt cgcagttgcg caaccccggc
     4261 gaccacgtgg tcgccgactt cgccggcttg ccggtgctcg cggtgcgcgg cgcggacgat
     4321 gcgatccggg tgttccacaa cgtctgcagg catcgcgccg ggccgatcgc gcaatgcgac
     4381 ggactggccg cgaagtcgct gcgctgccgc taccacggct ggacctacac gctggaaggc
     4441 cagttgcgcg cggcgcccga gatgaatggc gtggaggctt tcgatgtcgc cgacatccgc
     4501 ctgccgcagc tcgcggtgac ggtctggcag ggcctggtgt tcgcctccgt cgatgccacg
     4561 cgggcgcccg ggttcgatgc cttcgtcgcc ggcatcgatg cccgcctcgg cgctgatcgc
     4621 gggctggagc gctacggcaa ccatcatcgc gccagctacg acatcgcctg caactggaag
     4681 gtctatgtcg acaactacct ggagggttac cacgtccccc acgtgcaccc cggcctgaac
     4741 cgattgctcg actaccgcag ctatcgcacc gaactggaac gctggcattc gctgcagtgg
     4801 agcccgctgg aaagcgacgc cacgctctac ggcaacggcg acgcgctgta ctactggctg
     4861 tggccgaaca cgatgctcaa catcctgcct gggcgcatgc agaccaacac cgtgatcccg
     4921 ctcggcgtcg atcgctgccg tgtacttttc gacagttact acgcgcccga tgacagcgac
     4981 gccggcaagg cccgtcgcga ggccgacctc gccttcagcg acgaggtgca gcacgaagac
     5041 ctgggcatct gcgaggacgt gcagcgcggc ttcgcctccg gcagctacgt gccgggccgg
     5101 ctgaacccgc ttcgcgagtc gggcgtgcac catttccatg agatgctccg cgcggcctat
     5161 cgcaccagcg cgatctgatg ttttgctccc tcccctgctt gcaggggcgg gttggggagg
     5221 ggttgatgtc caccgatgga tcctcggcgc caagcgcctc gcaccccctc ccgacctccc
     5281 cctgcgcgca gggggaggag aagagacccc tcggcttgtg gatggccact gcgctggtgg
     5341 tcggcagcat gatcggcagc ggcgtcttcc tgttgccggc ggcgttggcg ccgtatggcg
     5401 cggccagcct ggtcggatgg gccatcgccc tctgcggtgc gctgctgctg gccgccacct
     5461 tcgccaagct cgcggtgcac tggccttgca cgggcggacc gtatgcctac gtgcgccgct
     5521 cgttcggcga tgtcgccggc ttcggcatca cgtggagtta ctggatctcg atctggtcgg
     5581 gcatggcggc gatcgcggtg gcct
//
LOCUS       DAOOCX010000147         5604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13621_length_5604_cov_5.656695, whole genome shotgun sequence.
ACCESSION   DAOOCX010000147 DAOOCX010000000
VERSION     DAOOCX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13621_length_5604_cov_5.656695"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /gene="glpD"
                     /locus_tag="PK399_10900"
     CDS             complement(<1..1291)
                     /gene="glpD"
                     /locus_tag="PK399_10900"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HPW13668.1"
                     /translation="MGEREMRQGSAKQLIQDHRFAHARFPACALFVFGLKRKKKKTTE
                     QRRTFFALTRHRRGRAHLPGASLTQSPNLGPIDGPATLSAAGSPRSAYDVVVVGGGIN
                     GVGIARDLAGRGQKVLLCEKDDLASHTSSSSTKLIHGGLRYLEYYEFSLVRKALAERE
                     LLLRSAPHIMWPLRFVMPHDPGMRPVWMIRAGLFLYDHLARREVLAGSRTIDLRRHPA
                     GAPLKRGLRKGFVYSDGWVDDARLVVLNAIDAAERGARVLTRWRCVDARRGAEGWQLR
                     LTSTAGETLEVGARALVNAAGPWAAQFLSEHAHAPKPKSLRLVKGSHIVVHKLFDHDH
                     AYIFQNPDKRIIFAIPYERDFTLIGTTDVEHRGPIGEARIDAAETDYLCEQASRYFEK
                     PVVPSDVVWSYSGVRPLLDDDSGDPSAVTRDYALELDT"
     gene            1290..2057
                     /locus_tag="PK399_10905"
     CDS             1290..2057
                     /locus_tag="PK399_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPW13669.1"
                     /translation="MTPNPRQSKVLDEVRARGSVTVDALAEQFGVTLQTVRRDVALLA
                     DAGLLARFHGGVRVAESTTENIAYRQRQQLNEQAKLRIARAVAQAVPEGCSLIINIGT
                     TTEAIARELLRHKGLRVITNNLNVAAILSDNPDCEVIVAGGVVRSRDRGIVGEVTVEF
                     IRQFKVDIGLIGISGIEADGTLRDFDYREVNVARAIMTHSRQVWLAADHSKFNRPAMV
                     ELARFSELDRLFTDTAPPAPFPALLSEAGVQCVTCAP"
     gene            2054..3568
                     /gene="glpK"
                     /locus_tag="PK399_10910"
     CDS             2054..3568
                     /gene="glpK"
                     /locus_tag="PK399_10910"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010481306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPW13670.1"
                     /translation="MSGADHLLALDQGTSSSRSIVFDRQGHIVSMAQREFRQIFPRPG
                     WVEHDPLELWSTQLATAREALQSAGLTARDIASIGITNQRETTVVWNRRTGEPIHNAI
                     VWQDRRAEPTCIALRERGLEDLFRRRTGLVLDAYFSGTKLKWILDQVPGARTAAAAGE
                     LAFGTVDSWLMWHLTQGTADTPVHATDVSNASRTLMLNVHGNRWDDELLALLDIPREV
                     LPAVHPSSHVYGHTRADLFGAPIAIGGVAGDQQSALFGQACFKAGLAKNTYGTGCFML
                     MHTGTQFQTSTNGLITTSAAQPTDVPEFALEGSVFIGGAVVQWLRDGLQAIKGSAEVQ
                     TLAESVPDSGGVMFVPAFTGLGAPYWQAEARGAIVGLTRGSSVAHIARAALESIAFQS
                     AALLQAMSRDAVAAGGSPVAELRVDGGACVNNLLMQFQADLLGIPVVRPKVIETTALG
                     AAYLAGLSCGLFDGLDALAAQWQAERTFRPTLPRERAAELMARWERAVRQTVAP"
     gene            complement(3599..5038)
                     /locus_tag="PK399_10915"
     CDS             complement(3599..5038)
                     /locus_tag="PK399_10915"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HPW13671.1"
                     /translation="MNHTTSFDARRRWLLQSGASCASALGVGSLGNLLLGARPAHAAD
                     YKALVCIFLYGGNDGLNTIVPSDAARHAQYSGVRGALAIPRDRLVALPGTEFGLHPSL
                     AALSAAAAAGHIAPVFNVGPLYAPLTKAQYRATPSGAALKPDGLFSHSDQQVLWETAT
                     THSQERTGWGGRASAVLGTVNPVISVGGSARFGLTTLQSPLVLPGPGSTFGAYGLQPD
                     DLKWAPMAARKAAIDLMYAQPQDAALADAYSRMQRDAFAMSQRLGALVKVEPGKAGSI
                     AAIDSAFASITVSGRISSGLGRQLYQVAKLIAGNATVQGDRQIFFAQAGGFDTHAGQI
                     AGSATEGEHARRLQEVGDALAAFYTAMKNLGLGQSVTAFTQSDFGRTFKPNSSNGTDH
                     AWGNQHLVVGGAVRGGATYGTYPQLVLGGDDDVGTESWEQHGRWIPTTSVDQYAATLL
                     GWFGASDAQLDAALPNLRNFGTRRRLGFV"
     gene            complement(5059..>5604)
                     /locus_tag="PK399_10920"
     CDS             complement(5059..>5604)
                     /locus_tag="PK399_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13672.1"
                     /translation="GIVPVAPDGSAVLMFTSVLRGLNGQSDGDALSWWWGEELREHVF
                     RPPSVFSFYPPDYPVAGTALVGPSFGIHNANAALQRLNYLTYLLDWGGSAAAAGVPNA
                     IGTKVNLTAFTADAADAGKLVDRLSVIATGQPLPTATRAEIIKAVSWWTVSTDKTNWQ
                     INRAKMAAYLVFATPSYQVQR"
BASE COUNT          890 a         1900 c         1935 g          879 t
ORIGIN      
        1 cggtgtcgag ctcgagcgcg tagtcgcgcg tcaccgccga cggatcgccg gagtcgtcgt
       61 cgagcagcgg ccgcacgccg gaatagctcc agacgacgtc ggacggcacc acgggtttct
      121 cgaaataccg gctggcctgt tcgcagaggt agtccgtttc tgccgcatcg atgcgtgctt
      181 cgccaatggg gccgcgatgc tcgacgtcgg tcgtgccgat caatgtgaaa tcccgttcgt
      241 acgggatcgc gaagatgatg cgcttgtcgg ggttctggaa gatgtaggcg tggtcgtggt
      301 cgaagagctt gtgcacgacg atgtggctgc ccttgaccag acgcagtgat ttcggcttgg
      361 gtgcgtgagc atgctcgctg aggaactgcg ccgcccacgg gccggcggca ttgaccagcg
      421 cccgcgcacc gacttccagc gtctcgccgg ccgtgctggt cagccgcagt tgccagcctt
      481 cggccccgcg ccgtgcatcg acgcagcgcc agcgggtcag cacgcgggca ccgcgctcgg
      541 ccgcatcgat cgcgttcagc acgaccagcc tcgcgtcatc gacccatccg tccgaataga
      601 cgaagccctt gcgcaagccg cgcttcaagg gcgccccggc agggtgccgg cgcaggtcga
      661 tggttcgtga gccggccagc acttcgcgcc gcgccagatg gtcgtacagg aacaggcctg
      721 cccggatcat ccagaccggt cgcatgccgg ggtcgtgcgg catcacgaat cgcagcggcc
      781 acatgatgtg cggagcgctg cgcagcagca gttcccgctc ggccagcgcc ttgcgcacca
      841 gcgaaaactc gtagtactcg aggtagcgca gacccccgtg gatcagcttg gtcgacgaag
      901 acgaggtgtg cgacgcgaga tcgtccttct cgcacagcag caccttctgg ccgcgtccgg
      961 ccaggtcacg cgcgataccg acgccgttga tgcccccgcc gaccacgacc acgtcgtaag
     1021 ccgaccgtgg cgaaccggcg gcggacagcg tggcgggccc gtcgatggga ccgaggttcg
     1081 gcgactgcgt caaggaagct ccagggagat gggcgcgacc gcggcggtgc cgggtcaacg
     1141 caaaaaatgt tcgtctctgt tcggttgttt tcttcttttt tctttttaag ccgaaaacga
     1201 aaagcgcgca agcaggaaat cgagcatggg cgaatcggtg gtcttggata agctgcttcg
     1261 cggatccttg ccgcatctcg cgctcaccca tgacacccaa cccccgccaa tcgaaggtcc
     1321 tcgacgaagt ccgtgcccgt ggttccgtga cggtggacgc actggccgag cagttcggag
     1381 tgaccttgca gacggtgcgc cgggatgtcg cgctgctggc cgacgcgggc ctgctggcgc
     1441 gctttcacgg cggcgtgcgg gtggccgaat cgaccaccga gaacatcgcc taccgccagc
     1501 gtcagcagct caacgagcag gccaagctgc gcattgcgcg cgcggtggcg caggcagtgc
     1561 ccgagggctg ttcgctgatc atcaacatcg gcaccaccac cgaggcgatc gcccgcgagc
     1621 tgctgcgcca caaggggctg cgcgtgatca ccaacaacct caacgtggcg gcgatcctga
     1681 gcgacaaccc ggactgcgag gtgatcgtcg ccggcggcgt cgttcgctca cgcgaccgcg
     1741 gcatcgtcgg cgaggtgacg gtcgagttca tccgccagtt caaggtcgac atcgggctga
     1801 tcggcatctc cggcatcgag gccgacggca ccctgcgcga cttcgactac cgcgaggtca
     1861 atgtggcgcg ggccatcatg acgcactcgc ggcaggtgtg gctcgccgcc gatcacagca
     1921 agttcaaccg cccggccatg gtggagctgg cgcgtttttc ggaactcgat cgcctgttca
     1981 ccgacaccgc cccaccggcc ccgtttccgg cgctgctgag cgaggccggc gtgcagtgcg
     2041 tgacctgcgc accatgagcg gcgcggacca cctcctcgcg ctcgaccagg gcacgtcgag
     2101 ttcgcgcagc atcgtgttcg accggcaggg acacatcgtg tcgatggcgc agcgcgaatt
     2161 ccggcagatc ttcccgcggc ccggctgggt cgagcacgat ccgctcgagc tgtggtcgac
     2221 gcagctcgcc accgcgcgcg aggcactgca gtccgccggg ctgacggcac gcgacatcgc
     2281 gtcgatcggc atcaccaacc agcgcgagac cacggtggtg tggaatcgac gcaccggcga
     2341 gcccatccac aacgcgatcg tctggcagga ccggcgtgcc gaaccgacct gcatcgcgct
     2401 gcgcgagcgc gggctcgagg atctgtttcg ccgccggacc ggactggtgc tcgacgccta
     2461 tttctcgggc accaagctga agtggatcct cgaccaggtg cccggtgcac gcactgcggc
     2521 ggcggccggc gagctggctt tcggcaccgt cgacagctgg ttgatgtggc acctgacgca
     2581 aggcacggcg gacacgccgg tgcatgccac cgacgtcagc aacgcgtcgc gcacgttgat
     2641 gctgaatgtg cacggcaacc ggtgggacga cgagttgctc gcactgctcg acatcccgcg
     2701 cgaggtgctg ccggcggtgc atccgtcgag ccacgtctac ggccacacgc gggccgacct
     2761 gttcggcgca cccatcgcca tcggcggcgt cgcgggcgac cagcagagcg cattgttcgg
     2821 gcaggcgtgc ttcaaggccg gactcgcgaa gaacacctac ggcaccggat gcttcatgct
     2881 gatgcacacc ggcacgcagt tccagaccag caccaacggg ctgatcacga ccagcgcggc
     2941 gcagcccacc gacgtccccg agttcgccct cgagggcagc gtcttcatcg gcggcgcagt
     3001 ggtgcagtgg ctgcgcgacg ggctgcaggc gatcaagggc agcgcggaag tgcaaacgct
     3061 cgccgagagc gtgcccgaca gcggcggcgt gatgttcgtg ccggccttca ccggcctcgg
     3121 tgcgccgtac tggcaagccg aggcgcgcgg cgcgatcgtc gggctgacgc gcggcagttc
     3181 ggttgcgcac attgcgcgcg ccgcgctgga aagcatcgcg ttccagagcg cggcgctgct
     3241 gcaggccatg agccgcgacg cggtggccgc cggcggctca ccggtggccg aacttcgcgt
     3301 cgacggtggc gcctgcgtca acaacctgct gatgcagttc caggccgacc tgctgggcat
     3361 tccggtggtg cgtccgaagg tgatcgaaac gacagcgctc ggcgcggcct acctggcggg
     3421 cctgagctgc ggcctgttcg acgggctgga tgcgctggcg gcgcaatggc aggccgagcg
     3481 caccttcagg cccacgctgc cgcgcgagcg agctgccgaa ctgatggccc gctgggagcg
     3541 cgcggtgcgg cagacggtgg cgccgtagca gcgccaccgc ccgggacccg ccgaccggtc
     3601 agacgaagcc gagccgtctt cgcgtaccga agttgcgcag gttgggcagc gcggcgtcga
     3661 gttgcgcgtc gctggcaccg aaccagccca gcaaggtggc cgcgtactga tcgaccgaag
     3721 tcgtcggtat ccagcggccg tgctgctccc aggactcggt gccgacatcg tcgtcgcccc
     3781 ccagcaccag ttgcgggtag gtgccatagg tggcgccgcc gcgcaccgcg ccaccgacca
     3841 ccaggtgctg gttgccccag gcgtggtcgg tgccgttgct gctgttgggc ttgaacgttc
     3901 ggccgaagtc gctttgcgtg aaggcggtga ccgactggcc gaggccgagg ttcttcatcg
     3961 cggtgtagaa cgctgcgagc gcgtcgccga cttcctgcag gcgccgggcg tgctcgcctt
     4021 cggtggcgct tccggcgatc tgcccggcgt gcgtgtcgaa gccccctgcc tgcgcgaaga
     4081 agatctggcg gtcaccctgc accgtcgcgt tgcccgcgat cagcttggcc acctggtaca
     4141 gctgccggcc gaggccgctg gatatgcgcc cgctgacggt gatcgacgcg aaggccgagt
     4201 cgatcgccgc aatggagcct gccttgccgg gctcgacctt gaccagcgct cccaggcgct
     4261 gcgacatcgc gaatgcatcg cgctgcatgc gcgaatacgc gtcggccagc gcagcgtcct
     4321 gcggttgcgc gtacatcaga tcgatggccg ccttgcgcgc ggccatcggc gcccacttca
     4381 ggtcatcggg ctgcagtccg taagccccga acgtgcttcc cggccccggc agcaccagcg
     4441 gcgactgcag cgtggtgagc ccgaagcggg cgctgccacc gacggagatc accgggttca
     4501 ccgtacccag caccgccgat gcgcgaccgc cccagccggt gcgctcctgc gaatgggtgg
     4561 tcgcggtctc ccacagcacc tgctggtcgg aatgagagaa caggccatcg ggcttcagcg
     4621 ccgcgccgct gggcgtggcg cggtactggg ccttggtcag cggcgcatag agcggaccga
     4681 cgttgaacac cggcgcgatg tggcccgcgg ccgcggctgc ggacaacgca gccagcgacg
     4741 gatgcaggcc gaactcggtg ccgggcaagg ccaccagcct gtcgcgcgga atcgccagcg
     4801 cgccgcgcac gccgctgtac tgggcatggc gggcggcatc cgaagggacg atggtgttca
     4861 ggccatcgtt gcctccgtac aggaagatgc acaccagcgc cttgtagtcg gcggcgtgcg
     4921 cgggccgcgc gcccagcagc agattgccga ggctgccgac gccgagggca ctggcgcaac
     4981 tggcgcccga ttgcagcagc cagcgtcggc gcgcatcgaa tgaggtcgtg tggttcatga
     5041 gttttccttt cgaggcgctc agcgctgaac ctggtagctc ggcgtcgcga agaccaggta
     5101 ggccgccatc ttggcgcggt tgatctgcca gttggtcttg tcggtggaca cggtccacca
     5161 cgacacggcc ttgatgatct cggcgcgcgt cgcggttggc aatggctgac cggtggcgat
     5221 caccgacagc cgatcgacca gcttgcccgc atcggcggcg tcggctgtga atgccgtcag
     5281 gttgaccttg gtgccgatcg cgttgggcac gccggcagcg gccgcggagc cgccccagtc
     5341 gagcaggtag gtcaggtagt tcagccgctg cagcgccgcg ttggcgttgt ggatgccgaa
     5401 gctcgggccc accagcgccg tgcccgccac cgggtagtcc ggcggataga agctgaacac
     5461 cgacggcgga cggaagacat gctcgcgcag ttcctcgccc caccaccagc tcagtgcatc
     5521 gccgtcgctc tggccgttca ggccgcgcag cacgctggtg aacatcagca ccgccgatcc
     5581 gtcgggggcc acgggcacga tgcc
//
LOCUS       DAOOCX010000148         5585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13676_length_5585_cov_7.549729, whole genome shotgun sequence.
ACCESSION   DAOOCX010000148 DAOOCX010000000
VERSION     DAOOCX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13676_length_5585_cov_7.549729"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK399_10925"
     CDS             <1..714
                     /locus_tag="PK399_10925"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPW13673.1"
                     /translation="LAHSANNRLADSSYALEKKWNGLSPFGREVVREMNRVGMIVDVS
                     HLGDASAMEAIQLSRVPVIASHSALRHFTPDFERNISDDIAKAVAARGGVIQIPFGTA
                     FIDPASAADTQAHFRAINDFDRNNAALKAQGQPPKDRAAFDTAWDAAHPPRASTLVQV
                     LDQIDYGVKLLGIDHVGIGSDFDGVDGDLPPELKTVADFPHLVAGLQARGYKDADIRK
                     ILGGNLLRAWGAIEDGAGK"
     gene            complement(796..1116)
                     /locus_tag="PK399_10930"
     CDS             complement(796..1116)
                     /locus_tag="PK399_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HPW13674.1"
                     /translation="MCTRSPGPYGATPLPRGLRTGSEKRVLESPVSRAARTTHSNRSR
                     SITMGKGDRKTAKGKRYNASYGNARSHATTKAAGTAAASVVKKAATKTAVKAPAKKVV
                     AKKA"
     gene            complement(1172..1585)
                     /locus_tag="PK399_10935"
     CDS             complement(1172..1585)
                     /locus_tag="PK399_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HPW13675.1"
                     /translation="MNPRLHALFDRLGATGALLCAIHCALLPLLIALLPSLGLSAALA
                     PTFEVGFVLFATLFGLAVLVWSYRRHRAMRALWLLIPGLAVLWFGILYAPLHHSVVPH
                     AVAMTFGGTLVGLAHLANLRLNHGHVHDATCAHGH"
     gene            1666..3714
                     /locus_tag="PK399_10940"
     CDS             1666..3714
                     /locus_tag="PK399_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW13676.1"
                     /translation="MRPSHVAPRRSALTLALAILLAPVTQAAAQEATTSPSSDTRHAT
                     ATGGDNHIKDISSVVVSGSLLRDTAETLSKPVEVLAGERLDESRAATLGETVATLPGV
                     QSSNFGPGVGRPVIRGMDGPRVAVMSGGLSSQDVSTVSQDHAPAIEPFLADQIEVLKG
                     PSTLLYGSGAIGGAVNSVDGRIPEYAIENGFSGRAETRFSAGDQGGNTTMARIDGGSD
                     RFALHADAVYRHAPDYDTPQGRQANAYIDTRSGAVGGSLLGDWGFVGLSVSRFEDEYG
                     NPGEPGDPAAGERGVHLELRQDRYEAKGALRNLWGDGNGLRFSFAHTGYEHTEFEGDA
                     PGTIFNKNANEGRVEATFGSTQGWQGAAGLQGSASTFAAFGEEAFVPETSTRALGLFG
                     VARRSFGDFQLDLGARIDKVKSTPEGGDARSFNPLSLSLAGGWKLDEHWKLTANLDHA
                     ERAPVEEELFSDGPHIATLAYEIGNANLRKEAANQFELGLQYQADWVDAKVSAYYNRF
                     NDFIYLADTGDSWYWDEEDQLLPIREWTQGDASFRGVEGEATFHLAVNDSGDWDLRVF
                     GDAVRATLKDGGNVPRIVPSRLGAQLRWERGGWRASLGATRSNRQDKVAANETPTAGF
                     TLVDAHATRHFDTGNLSWELFADATNLTNQEGRVHTSFLKDAVMLPGRSVALGVRLFF
                     "
     gene            complement(3770..>4213)
                     /locus_tag="PK399_10945"
     CDS             complement(3770..>4213)
                     /locus_tag="PK399_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPW13677.1"
                     /translation="DAAGFVTAVERACSERGLRLTDIRARVLGLVADAGKPIKAYDLL
                     DKVREGEGAGAAAPPTVYRALDFLLANGFIHKLESVNAFVACHHPNAAQHSAPFLICD
                     GCHKAVELEDEHVVATLDAQARALGFSPQTQTLEVHGLCAECAGK"
     assembly_gap    4214..4313
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4314..>5585)
                     /gene="gltX"
                     /locus_tag="PK399_10950"
     CDS             complement(<4314..>5585)
                     /gene="gltX"
                     /locus_tag="PK399_10950"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPW13678.1"
                     /translation="RTRFAPSPTGYLHIGGARTALYCWLESRHKGGEFILRIEDTDRE
                     RSTQAAIDAILQAMAWLGLDYDEGPIYQTHRLDRYKEVAEQLVASGHAYYAYETKQDL
                     EAMREAAMAAGEKPRYNGAYRDANAGWRDDPNRVIRFKNPLDGVVAWDDKVKGRIEIA
                     NSELDDLVIFRSDGYATYNFAVVVDDIDMRITDVVRGDDHVNNTPRQINIYHALGAPV
                     PNFAHLPMILDEQGAKLSKRTGAADVMQYRDAGYLPHALLNYLVRLGWSHGDQEVFSI
                     AEMQSLFDLADVNAKAARLDMAKLGWLNQQYLKSDDAESVAGHLQWHLQQAGYDLAKG
                     PKAADIVIALRERAQTLKDMAEKSAVWFGPLTVYDEAAVAKHLKEAAREPLAEMRVRL
                     AQLTDWSPEPIHDVVKAVSEALGIGMGKIAQP"
BASE COUNT          905 a         1892 c         1815 g          873 t
ORIGIN      
        1 ctggcgcaca gcgccaacaa ccggctcgcg gattcgtcct acgcgctcga gaagaagtgg
       61 aatggcctca gcccgttcgg ccgcgaggtg gtgcgcgaga tgaaccgggt cggcatgatc
      121 gtggacgtgt cgcacctcgg cgatgcctcg gcgatggagg cgatccaact gagccgagtg
      181 ccggtgatcg ccagccattc cgcgttacgc catttcaccc ccgatttcga gcgcaacatc
      241 agtgacgaca tcgccaaggc cgtggcggcg aggggcggcg tgatccagat cccgttcggc
      301 accgccttca tcgacccggc cagcgccgcc gatacccagg cgcatttccg cgcgatcaac
      361 gacttcgacc gcaacaacgc cgccctgaag gcgcaaggcc agccgccgaa ggaccgcgcc
      421 gcgttcgaca cggcctggga cgccgcccat ccgccgcgcg caagcacctt ggtgcaggtc
      481 ctcgaccaga tcgactacgg ggtgaagctg ctcggtatcg accatgtcgg gatcggctcg
      541 gacttcgacg gcgtcgacgg cgacctgccg ccggaactga agacggtggc cgatttcccg
      601 cacctggtcg ccggcctgca ggcgcgcggc tacaaggacg cggacatccg caagatcctg
      661 ggcggcaacc tgctgcgcgc ctggggcgcg atcgaggacg gcgcaggcaa gtaagcaacg
      721 caggagtcac cgcccacaac gcaagaaccc cgccgaggcg gggttcttgt cgtccatcgc
      781 gggaatccgg cgtggttacg ccttcttcgc gacgaccttc ttggccggag ccttgaccgc
      841 ggtcttggtc gcggccttct tcaccacgga agcagcggcg gtgccggcgg ccttggtggt
      901 tgcgtgcgag cgtgcattgc catagctggc gttgtagcgc ttgcccttgg cggtcttgcg
      961 gtcgcccttg cccatcgtga tgctcctgga tcgattcgag tgtgtggttc gcgccgcgcg
     1021 cgagaccggc gattctagca cgcgcttttc gctccccgtt cttaaccccc ttggtaaggg
     1081 ggtggccccg taggggccgg gggatcgggt gcacatagcg gggcccaaga tttcgctcgc
     1141 gaaatcttgg gattgacgat gcgcagcagc gtcaatgccc gtgcgcgcag gtcgcgtcgt
     1201 ggacgtggcc gtggttgagc cgcaggttgg ccaggtgggc caggcccacc agggtgccgc
     1261 caaaggtcat cgccaccgca tgcggcacca ccgaatggtg cagcggcgcg tacaggatcc
     1321 cgaaccagag caccgccagc cccgggatca gcagccacaa cgcgcgcatg gcgcggtggc
     1381 ggcgatagct ccacaccagc accgccaggc cgaacagggt ggcgaacagc acgaacccca
     1441 cttcgaaggt cggcgccagc gccgccgaca ggccgagcga cggcagcagc gcgatcagga
     1501 gcggcagcag cgcgcagtgg atcgcgcaca gcaacgcacc cgtggcaccg aggcggtcga
     1561 acagggcgtg caggcgtgga ttcattcggg ggtgacttca ggcacgggtc gggatcgtgt
     1621 tttgatatgt tataacatct accatcgcca ccacaagctt accccatgcg cccatcccac
     1681 gttgcccctc gacgatccgc cctgaccctg gccctggcga tactcctggc gccagtcacc
     1741 caagcggctg cccaagaggc cacgacgtct ccctccagcg acacccgcca tgcgaccgcc
     1801 acgggcggcg ataaccacat caaggacatc tccagtgtgg tcgtctccgg cagcctgctg
     1861 cgcgataccg ccgaaaccct gagcaagccg gtggaagtcc ttgccgggga acgcctcgac
     1921 gagagccggg ctgcgacgct gggcgagacc gtcgccaccc tccccggcgt ccagagctcc
     1981 aacttcggcc ccggcgtggg ccgcccggtc atccgcggca tggacggccc ccgtgtcgcc
     2041 gtgatgagcg gcggcctgtc cagccaggac gtgtcgaccg tcagccagga ccatgcaccc
     2101 gcgatcgagc cgttcctggc cgatcagatc gaggtcctga agggcccctc caccctgctt
     2161 tacggcagcg gcgcgatcgg cggcgcggtg aactcggtgg atggccgcat ccccgagtac
     2221 gccatcgaga acggtttcag cggccgggcc gaaacgcggt tctccgccgg cgaccagggc
     2281 ggcaacacca ccatggcccg gatcgatggc ggcagcgaca ggttcgcgct gcacgccgac
     2341 gcggtctacc gccatgcacc ggactacgac accccacagg gccgccaggc gaacgcctac
     2401 atcgacaccc gctccggcgc ggtgggcggc tcgctactgg gcgactgggg cttcgtgggc
     2461 ctgtcggtct cacgcttcga ggacgagtac ggcaatccgg gcgaacccgg cgatcccgca
     2521 gccggcgagc gcggcgtcca cctggaactg cgccaggacc gctacgaggc caagggcgcg
     2581 ctgcgcaacc tgtggggcga cggcaacggc ctgcgcttct ccttcgccca caccggctac
     2641 gaacacaccg agttcgaggg cgacgcgccg ggcaccatct tcaacaagaa cgccaacgaa
     2701 gggcgcgtgg aagcgacctt cggcagcacc cagggctggc agggcgccgc aggcctgcag
     2761 ggctccgcga gcaccttcgc tgcgttcggc gaggaagcgt tcgtgcccga gaccagcacg
     2821 cgtgcgctgg gcctgttcgg ggttgcgcgg cgcagcttcg gcgacttcca gctcgacctt
     2881 ggcgcgcgca tcgacaaggt gaagtcgacc ccggaaggcg gcgatgcccg cagcttcaac
     2941 ccgctcagcc tgtcgctggc cggcggatgg aagctggatg aacactggaa gctgaccgcc
     3001 aacctcgacc acgccgaacg tgcgccggtc gaggaggagc tgttctccga tggcccgcac
     3061 atcgccaccc tcgcctacga gatcggcaac gcgaacctgc gcaaggaagc cgccaaccag
     3121 ttcgagcttg gcctgcaata ccaggccgac tgggtggatg cgaaggtctc cgcctactac
     3181 aaccgcttca acgacttcat ctacctggcc gacaccggcg acagctggta ttgggacgaa
     3241 gaagaccagt tgctgccgat ccgggagtgg acgcaaggcg acgccagctt ccgcggcgtg
     3301 gaaggcgaag cgaccttcca cctggccgtg aacgacagcg gcgactggga cctgcgcgtg
     3361 ttcggcgacg ccgtgcgcgc tacgttgaag gacggcggca acgtgccgcg catcgtgccg
     3421 tcgcgacttg gcgcacaact gcgctgggaa cgcggaggct ggcgcgcctc gctgggcgca
     3481 acccgctcca accggcagga caaggtggcc gccaacgaaa caccgaccgc cggcttcacc
     3541 ctggtcgacg cacacgccac ccgccacttc gacaccggca acctgagctg ggagttgttc
     3601 gcggacgcca ccaacctcac caaccaagag gggcgcgtgc atacgtcgtt cctcaaggac
     3661 gcggtgatgc tccctggccg cagcgtggca ctgggcgtgc gcctgttctt ctgatcgccg
     3721 ccgcccctgc ggtcgatccg cgcctgcccc tgtccagggg caggcgtttt catttgccgg
     3781 cgcactccgc gcacaggccg tgcacttcca gcgtctgcgt ctgcggactg aagcccagcg
     3841 cgcgcgcctg cgcgtccagg gtggcgacga cgtgttcgtc ttccagttcg accgccttgt
     3901 ggcagccatc gcagatcagg aaaggtgccg aatgctgggc cgcgttcgga tggtggcagg
     3961 cgacgaaggc gttgaccgat tccagcttgt ggatgaagcc attggccagc aggaaatcca
     4021 gcgcgcgata gaccgtcggc ggcgcagcgg caccggcgcc ctcgccttcg cggaccttgt
     4081 cgagcaggtc gtacgccttg atcggcttgc cggcatcggc gaccaggcca agcacgcgtg
     4141 cgcggatgtc ggtcagccgc aggccgcgtt cgctgcaggc gcgctccacc gccgtcacga
     4201 agccggcggc atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggctgc
     4321 gcgatcttgc ccatgccgat gccgagtgcc tcggataccg ccttcaccac atcgtggatc
     4381 ggctcgggcg accagtccgt caattgggca agccgcacgc gcatctccgc caacggctca
     4441 cgcgctgctt ccttgagatg cttggccacc gccgcttcgt cgtagacggt caatgggccg
     4501 aaccacaccg cggacttttc cgccatgtcc ttcagcgtct gcgcgcgctc acgcaacgcg
     4561 atgacgatgt cggccgcctt cgggcccttc gccaggtcgt agcccgcctg ttgcagatgc
     4621 cattggagat gcccggccac tgactcggca tcatcggatt tcaggtattg ctggttgagc
     4681 cagcccagct tggccatgtc caggcgcgcg gccttcgcgt tcacgtcggc gaggtcgaac
     4741 agcgactgca tttcggcgat ggagaacact tcctggtcgc catgcgacca gcccaggcgc
     4801 accaggtaat tcagcagtgc gtgcggcagg tagccggcgt cgcggtactg catgacgtcg
     4861 gccgcaccgg tgcgcttact gagcttggcg ccctgctcgt ccaggatcat cggcaggtgg
     4921 gcgaagttcg gcaccggcgc gcccagcgcg tggtagatgt tgatctggcg cggggtgttg
     4981 ttgacgtggt cgtcgccgcg caccacgtcg gtgatgcgca tgtcgatgtc gtccaccacc
     5041 accgcgaagt tgtaggtggc gtaaccgtcg ctgcggaaga tcaccaggtc gtcgagctcg
     5101 ctgttggcga tctcgatgcg acccttgacc ttgtcgtccc aggccaccac gccgtccagc
     5161 ggattcttga atcggatcac ccggttcgga tcgtcgcgcc agccggcgtt cgcatcgcga
     5221 tacgcgccgt tgtagcgcgg cttctcgccc gccgccatcg cggcttcgcg cattgcctcg
     5281 agatcctgct tggtctcgta ggcgtagtac gcatggcccg aagccaccag ttgctcggcg
     5341 acctccttgt agcggtcgag gcgatgggtc tggtagatcg ggccctcgtc gtagtccagg
     5401 cccagccacg ccatcgcctg caggatggcg tcgatcgccg cctgcgtgct gcgctcgcgg
     5461 tcggtgtcct cgatgcgcag gatgaactcg ccgcccttgt ggcgcgattc cagccagcag
     5521 tacaacgcgg tgcgcgcgcc gccgatgtgc aggtagccgg tgggactggg ggcgaagcgg
     5581 gtgcg
//
LOCUS       DAOOCX010000149         5547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13791_length_5547_cov_8.462127, whole genome shotgun sequence.
ACCESSION   DAOOCX010000149 DAOOCX010000000
VERSION     DAOOCX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13791_length_5547_cov_8.462127"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="PK399_10955"
     CDS             <1..1574
                     /locus_tag="PK399_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPW13679.1"
                     /translation="RTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKGRKAARHATS
                     DWMALEKERGISVTSSVMQFPYEGRIVNLLDTPGHADFGEDTYRVLTAVDSALMVIDV
                     AKGVEERTIKLMEVCRLRDTPIMTFVNKLDREGKDPIELLDEIESVLGIQCAPVTWPI
                     GMGQRLKGVVHLVTGEVHLYEPGRNFTRQDSTIFPSLDAPGVAERIGADMLAEVRDQL
                     ELVQGASHPFNREAYLEGMQSPVFFGSGVNNFGVQPLLDFFVEHAPSPQARATTTRLV
                     EAAEPKLTGFVFKIQANMDPMHRDRVAFMRICSGKFEAGMKAFQPRTGKEVKLANALT
                     FMASDREIAESAFPGDVIGIHNHGTISIGDTFTEGEKIAFTGIPNFAPELFRRARLRD
                     PLKLKQLQKGLAQLSEEGATQFFRPLMSNDLILGAVGVLQFEVVAYRLKDEYGVEASF
                     EQVAVTTARWIKCGDAKKLEEFREKNAMNLALDAAGHLVYLAPTRVNLQLAQERAPSV
                     EFLATREHAQAVAVA"
     gene            complement(1584..2219)
                     /locus_tag="PK399_10960"
     CDS             complement(1584..2219)
                     /locus_tag="PK399_10960"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HPW13680.1"
                     /translation="MNDIARPAANPRKTTSIRKLGSIDIAALLAAVQAIPEAVWDAEN
                     ADKPNKFEALDKTRHIVFRFVDSTRDWRGSHDRLRWEEWKALLQPVMDQAVADYGYAR
                     GVFPRVMLARMGPGGVIQPHIDANPAAKWPHKIHVPLTTNEKVGFVIGNTVHHFPVGE
                     AVEVNNLGPHAVYNEGDSERIHLIFEYYDADQPEPDWLAPFLEQDAARRQA"
     gene            complement(2228..2851)
                     /locus_tag="PK399_10965"
     CDS             complement(2228..2851)
                     /locus_tag="PK399_10965"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HPW13681.1"
                     /translation="MIISHQHRFIFAAVPKTGTHSVRQALREHMSQDDIEQVGLFVNK
                     RFPYEELAAIRHGHLSLQQVRPFLGEDMFAGYFKFAFVRNPFDRFVSYCAFMSRDSGH
                     FATNPQAVMRYFLFDAPPEQHILFQPQSSLLVDGDGRLLTDMVGRVETMQDSYEAICA
                     RIGIPARVLDQVNSSRRGDYRSYYTDELREAVATRYRNDIDLFDYRF"
     gene            2939..3589
                     /locus_tag="PK399_10970"
     CDS             2939..3589
                     /locus_tag="PK399_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPW13682.1"
                     /translation="MPPTIKHIDPREEFASALTHGLGATAALAGGAVLIALAALHGNG
                     WQLGAAIVFGVALLLLYVASTLYHAVQHPIAKGRLKVFDHCAIYVLIAGTYTPFTLIG
                     LRGPVGWWLFGAIWTLALGGVVFKLFYTGRFKRLSTLIYIAMGWLIVVAAKPMLAALD
                     AWTLGWLLAGGVSYTLGTWFYHRESIRYSHAIWHLFVLGGSISHYIAVLAHVVPAR"
     gene            complement(3608..4036)
                     /locus_tag="PK399_10975"
     CDS             complement(3608..4036)
                     /locus_tag="PK399_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPW13683.1"
                     /translation="MRTVRQLLESKSAEIHAVPPDAPVIEAIRLMADHHVGALLVMEG
                     ARLAGILSERDYARKVVLQGRSSKDTPVRDIMTATVITVAPGDSAEHCMQVVTDHRIR
                     HLPVLDGDTVLGVVSIGDLVRAVIEEQRQELDHLQRYIAS"
     gene            complement(3996..5036)
                     /locus_tag="PK399_10980"
     CDS             complement(3996..5036)
                     /locus_tag="PK399_10980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW13684.1"
                     /translation="MHRIAPDDCCLTIVIAAFNEAEALPVLQPRIATALALAEAGGLE
                     ARVLYVDDGSRDATWDVLRGFAAADSRVALLRLSRNFGKEAALTAGLDRVERGAAFIL
                     DADGQDPPELLPRFVAKWREGFDDIHGTRVEREGEGWLKRATAHGFYRLIGSLSKTPV
                     PRDTGDFRLLSPRALAALRQLRERHRFMKGLFGWVGFNQVAIPYQREARVAGRSKFNF
                     WALWNFALEGITSFSTAPLRMATYLGIATALVAFVYGSWIILKALLWGDPVAGWPTMM
                     SVILFLGGVQLVALGLIGEYLGRLYEESKQRPLYLVDDWRPATGVSSGQVPDPTGASD
                     AHGTAAAGVEIR"
     gene            complement(5070..>5547)
                     /locus_tag="PK399_10985"
     CDS             complement(5070..>5547)
                     /locus_tag="PK399_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPW13685.1"
                     /translation="FAAIEKAQAHNEKMVERAEKRGKPVRRLRGASTISQQTAKNLFL
                     WQGSGPTRWLRKGLEVWYTALIEALWPKTRILEVYANIAEFGDGIYGGQAAARSFFRK
                     DAAKLTATEAARLAAVLPSPKRYSASRPGPYVQRRTRAIERQMRAIGGTSYLRTLD"
BASE COUNT          900 a         1887 c         1826 g          934 t
ORIGIN      
        1 gtcgcacctt cgccatcatt tcccaccccg acgccggcaa gaccacgctg accgagaagc
       61 tgctgctgtt cggcggcgcg atccagatgg ccggttcggt caagggccgc aaggccgccc
      121 gccacgcgac ctccgactgg atggcgctgg aaaaggaacg cgggatctcc gtcaccagct
      181 cggtgatgca gttcccgtac gagggccgca tcgtcaacct gctggacacc ccaggccacg
      241 ccgacttcgg tgaggacacc tatcgcgtgc tgaccgcggt ggacagcgcg ctgatggtga
      301 tcgacgtcgc caagggcgtc gaggaacgca cgatcaagct gatggaggtg tgtcgcctgc
      361 gcgacacccc gatcatgact ttcgtgaaca agctggaccg cgagggcaag gatccgatcg
      421 agctgctgga cgagatcgaa tcggtgctcg gcatccagtg tgcgccggtc acctggccga
      481 tcggcatggg ccagcgcctc aagggcgtgg tccacctggt caccggcgag gtgcacctgt
      541 acgagccggg gcgcaacttc acccgccagg attccaccat cttcccgtcg ctggacgcgc
      601 ccggcgtggc cgagcgcatc ggcgccgaca tgctggccga agtgcgcgac cagctcgaac
      661 tggtgcaggg cgcatcgcat ccgttcaatc gcgaggccta cctggagggc atgcagtcgc
      721 cggtgttctt cggctccggc gtcaacaact tcggcgtgca accgctgctg gatttcttcg
      781 tcgagcatgc gccgtcgccg caggcccgcg cgaccaccac gcggctggtc gaagcggcgg
      841 agcccaagct gaccggcttc gtgttcaaga tccaggccaa catggacccg atgcaccgcg
      901 accgcgtcgc cttcatgcgg atctgctcgg gcaagttcga ggccggcatg aaggcgttcc
      961 agccgcgtac cggcaaggag gtgaagctgg cgaatgcgct gaccttcatg gccagcgacc
     1021 gcgagatcgc cgaatccgcc tttcccgggg acgtgatcgg catccacaac cacggcacca
     1081 tctcgatcgg cgacaccttc actgaaggcg agaagatcgc gttcaccggc atcccgaact
     1141 tcgcgcccga actgttccgc cgcgcgcgcc tgcgcgaccc gctcaagctc aagcaactgc
     1201 agaagggcct ggcgcagctg agcgaggaag gcgcgaccca gttcttccgc ccgctgatga
     1261 gcaacgacct gatcctgggc gcggtcggcg tgctgcagtt cgaagtggtc gcgtatcggc
     1321 tgaaggacga atacggcgtg gaggccagct tcgagcaggt cgcggtcacc accgcgcgct
     1381 ggatcaagtg cggcgacgcg aagaagctgg aggagttccg cgagaagaac gcgatgaacc
     1441 tcgcgctcga tgccgccggc cacctggtct acctggcgcc gacgcgggtc aacctgcagc
     1501 tggcgcagga acgcgcgccc tcggtggagt tcctggccac ccgcgagcac gcgcaggcgg
     1561 tggcggtcgc ctgatcgccg cggtcaggcc tgcctgcggg ccgcgtcctg ttcgaggaac
     1621 ggcgccagcc agtccggttc cggctggtcg gcgtcgtagt actcgaagat caggtggatg
     1681 cgctcgctgt cgccttcgtt gtacacggca tgcggcccga ggttgttgac ctccaccgcc
     1741 tcgcccaccg ggaaatggtg gacggtgttg ccgatcacga agccgacctt ctcgttggtg
     1801 gtcaacggca cgtggatctt gtgcggccat ttcgccgccg ggttcgcatc gatgtgcggc
     1861 tggatgacgc cgcccggccc catccgcgcc agcatcaccc gcgggaatac gccgcgcgca
     1921 tacccgtagt cggccaccgc ctggtccatc accggctgca gcaacgcctt ccattcttcc
     1981 cagcgcagcc ggtcgtgcga accgcgccag tcgcgggtgc tgtcgacgaa acggaagacg
     2041 atgtggcggg tcttgtcgag cgcttcgaac ttgttcggct tgtcggcgtt ctccgcgtcc
     2101 cacaccgctt ccgggatcgc ctgcaccgcg gcaagcaggg ccgcgatgtc gatcgagccg
     2161 agcttgcgga tcgacgtagt cttgcgcgga ttggcggccg gccgtgcgat gtcgttcatg
     2221 ggcgagctca gaaccggtag tcgaacaggt cgatgtcgtt gcggtagcgc gtggccactg
     2281 cctcccgcaa ctcgtccgtg tagtaactgc gatagtcgcc gcggcggctg ctgttgacct
     2341 ggtccagcac gcgggcggga atgccgatgc gtgcgcagat cgcctcgtag gaatcctgca
     2401 tggtctcgac gcgcccgacc atgtcggtga gcaggcgtcc atcgccatcg accagcaggc
     2461 tgctctgcgg ctggaacagg atgtgctgtt cgggcggcgc atcgaacagg aaatagcgca
     2521 tcaccgcctg cgggttggtg gcgaagtgcc cgctgtcgcg actcatgaag gcgcagtacg
     2581 acacgaagcg gtcgaacgga ttgcgcacga aggcgaactt gaaatagccc gcgaacatgt
     2641 cctcgccgag gaacggacgc acctgttgca gcgaaaggtg gccgtggcgg atcgccgcca
     2701 gttcctcgta cgggaaccgc ttgttcacga acagcccgac ctgctcgatg tcgtcctggc
     2761 tcatgtgctc gcgcaatgcc tggcggaccg aatgggtgcc ggtcttgggg accgcggcga
     2821 agatgaaacg atgctggtgc gagatgatca tgcgggccgg cgcaaactgc aggccgccat
     2881 tttagcctgc ccacgaccgc cgagcatttg ctccaccccc gcccccgaag ctgcccgcat
     2941 gcctcccacg atcaaacaca tcgatccgcg cgaagagttc gccagtgccc tgacccatgg
     3001 cctgggcgcc accgcggccc tggcaggcgg cgcggtactc atcgccctgg ccgccctgca
     3061 tggcaatggc tggcaactgg gcgcggcgat cgtgttcggc gtggcgctgt tgctgctgta
     3121 cgtggcatcg accctgtatc acgcggtcca gcaccccatc gccaagggac gcctcaaggt
     3181 gttcgaccat tgcgcgatct acgtgctgat cgccggcacc tacaccccgt tcaccctgat
     3241 tggcctgcgc ggtccggtgg gctggtggct gttcggcgcg atctggaccc tggcgctcgg
     3301 cggggtggtc ttcaagctgt tctacacggg gcgcttcaag cgcctgtcca cgctcatcta
     3361 catcgcgatg ggctggctga tcgtggtcgc ggcgaagccg atgctggccg cgctggatgc
     3421 gtggacattg ggctggctgc tcgcgggcgg cgtgtcctac acgctgggta cctggttcta
     3481 ccaccgcgag tcgatccgct actcgcacgc gatctggcac ctgttcgtgc tgggcggcag
     3541 catcagccac tacatcgcgg tgctcgcgca cgtggtgccg gcacgctgaa cggccgcgtt
     3601 gggttcgcta cgaggcgatg tagcgctgca ggtggtccag ttcctgtcgc tgctcctcga
     3661 tcaccgcgcg caccaggtcg ccgatggaga ccaccccgag cacggtatcg ccgtccagca
     3721 cgggcaggtg gcggatgcga tggtcggtca ccacctgcat gcagtgctcg gcgctgtcgc
     3781 cgggggccac ggtgatgacc gtggcggtca tgatgtcgcg caccggcgtg tccttcgagg
     3841 agcgcccctg cagcacgacc ttccgcgcat agtcgcgctc ggagaggatg ccggccagcc
     3901 gcgctccctc catcaccagc agggcaccga catggtggtc ggccatcaac cggatcgcct
     3961 cgatcaccgg cgcatccggc gggaccgcat ggatttcagc ggatttcgac tccagcagct
     4021 gccgtaccgt gcgcatcgct cgctcctgtc ggatcgggaa cctgtcccga ggatactccg
     4081 gtcgcgggac gccaatcatc gaccaggtac aagggccgct gcttggactc ctcgtacagc
     4141 cgccccaggt attcgccgat gaggccaagc gccaccagtt gcacgccccc caggaacagg
     4201 atgaccgaca tcatggttgg ccagcctgcc accgggtcgc cccacagcag ggccttgagg
     4261 atgatccagc tgccgtacac gaacgcaacc agcgcggtgg cgatgccgag ataggtggcc
     4321 atccgcagcg gcgcggtgct gaagctggtg atgccttcca gcgcgaagtt ccacaaggcc
     4381 cagaaattga acttgctgcg accggcgacg cgcgcctcgc gctggtacgg gatcgcgacc
     4441 tggttgaagc ccacccagcc gaacagtccc ttcatgaagc gatggcgttc gcgcaactgc
     4501 cgcagcgccg ccagcgcacg cggcgaaagc agccggaaat cgccggtatc gcgcggcacc
     4561 ggggtcttcg acagcgaccc gatcaagcgg tagaagccgt gtgcggtggc ccgcttcagc
     4621 cagccctcgc cttcccgctc cacgcgggtg ccgtggatgt cgtcgaatcc ctcgcgccat
     4681 ttcgccacga accgcggcaa cagttccggg ggatcctggc cgtcggcatc gaggatgaag
     4741 gccgcgccgc gctccacccg atccaggccg gcggtcaacg cagcctcctt gccgaaattc
     4801 cgcgacaacc gcagcagcgc cacccgcgag tcggcggcag cgaagccgcg caacacgtcc
     4861 caggttgcat cgcggctgcc gtcgtcgaca tacagcaccc gcgcctccag gcctcctgct
     4921 tcggccaggg cgagcgccgt cgcgatgcgc ggctgcaaca ccggcaacgc ctcggcctcg
     4981 ttgaaggcgg cgatcacgat ggtcaggcag cagtcgtcgg gcgcgatgcg gtgcatgtgt
     5041 cgatggtatg ccagcgtcgc tgcgggcgct cagtccagcg tgcgcaggta tgaggtgccg
     5101 ccgatcgcgc gcatctgccg ctcgatcgcg cgcgtgcgcc gttgcacgta ggggccgggc
     5161 ctgctcgcgc tgtagcgttt cgggctgggc aacacggcgg cgagccgggc ggcttcggtt
     5221 gcggtcagct tcgccgcatc cttgcggaag aagctgcgcg ccgccgcttg cccgccatag
     5281 atgccgtcac cgaactcggc gatgttcgcg tagacctcca ggatccgcgt cttcggccac
     5341 agcgcctcga tcaacgcggt gtaccagacc tccaggccct tgcgtagcca gcgggtcggg
     5401 ccgctgccct gccacaggaa caggttcttg gccgtctgct ggctgatcgt gctggccccg
     5461 cgcaagcgac gcaccggctt cccgcgcttc tccgcacgct cgaccatctt ctcgttgtgc
     5521 gcctgcgctt tctcgatggc ggcgaaa
//
LOCUS       DAOOCX010000150         5519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13887_length_5519_cov_8.303990, whole genome shotgun sequence.
ACCESSION   DAOOCX010000150 DAOOCX010000000
VERSION     DAOOCX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13887_length_5519_cov_8.303990"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /gene="ftsA"
                     /locus_tag="PK399_10990"
     CDS             <1..610
                     /gene="ftsA"
                     /locus_tag="PK399_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPW13686.1"
                     /translation="VDMGAGTTDIAVFVQGAISHTASLPVAGDKVTEDIAHMLRTPTP
                     EAEQIKVRYACALAQLARAEESIQVPSVGDRPPRRMPRHALAQAVQARYEEIFEMVQA
                     ELRRSGFEQLVRAGMVLTGGASKMEGVVELAEEMLQMPVRIGIPQHVSGLGEVVGNPV
                     HATGVGLLLMGSQIENPRRPQLRDGRAFGWMDKLKNWYRGQF"
     gene            729..1976
                     /gene="ftsZ"
                     /locus_tag="PK399_10995"
     CDS             729..1976
                     /gene="ftsZ"
                     /locus_tag="PK399_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017170527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPW13687.1"
                     /translation="MAQTFELVEKMAPNAVIKVIGVGGGGGNAVAHMVNSSVDGVEFI
                     TANTDSQAIKNCGAKLQLQLGGNVTKGLGAGANPEVGRQAALEDRDTIIDALTGADMV
                     FITAGMGGGTGTGAAPVVAQVAKEMGILTVAVVTKPFPFEGRRRMQVALKGIEELSQH
                     CDSLITIPNEKLITVLGRNATMIQAFRAANDVLLGAVQGIADLIVRPGLINVDFADVR
                     TVMSEMGLAMMGSGSARGDDRAQAAAEGAIQNPLLDDVNLSGANGILVNITAGPDFTM
                     AEFDEVGRTVEQFASEDATVVIGTVLDPEMQDEVRVTVVATGLNRNTAGGRQPAPRSE
                     ARYDSGFDAPRRPQVQLINTQGKRDGTTGLMIDEVSDPFVPGANTGFVGGLRRNVDAG
                     PAVAELPKDDYLDIPAFLRRQAD"
     gene            2215..3129
                     /gene="lpxC"
                     /locus_tag="PK399_11000"
     CDS             2215..3129
                     /gene="lpxC"
                     /locus_tag="PK399_11000"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPW13688.1"
                     /translation="MLRQRTLKNLIRATGVGLHGGEKVFLTLRPAPIDTGIVFRRIDL
                     DPVVEIPASAALVTETTLCTGLSCEGAKVQTVEHLMSALAGLGIDNIYIELTAPEVPI
                     MDGSAGPFVFLLQSAGIAEQNAPKRFLRIRKHVEVRDGDKVASFSPHDGFRVGFTVKF
                     NHPAIPDTLSRAEVDFSTSAYVSEVSRARTFGFMQDLEYMRERNLGLGGSMDNAIVLD
                     EFRVLNEDGLRYADEFVRHKILDAVGDLYLAGRPIIGAYEGYKSGHALNNRLVRALLA
                     DAEAWEEVSFTDPVAAPPVVYGDAIPAT"
     gene            complement(3255..3746)
                     /locus_tag="PK399_11005"
     CDS             complement(3255..3746)
                     /locus_tag="PK399_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014035989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPW13689.1"
                     /translation="MPGSRSTPPAGATPPSGPRAALQALLDGSSGGTLRRALWLDAVD
                     QLLRPHLPPVLAAHARLANVRGDKLVFVVDGPVWHAKLRLATQELVDAARSVGLDVAG
                     LTVRTTTQPLRPIPPAATTAPPMSAAGQSGLAAALALLRSDAPDVSEQRAGGNRKPGR
                     RGT"
     gene            3752..4678
                     /locus_tag="PK399_11010"
     CDS             3752..4678
                     /locus_tag="PK399_11010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW13690.1"
                     /translation="MTETSKSHDTRRRVIAQAQAFRFWAMRRPLAALGVLLGAGLLLG
                     AGGRSVVGMAEVEAMQAAEANRQAELAKVRRDAQREVNALAARLAELQAQANRLNALG
                     ARLTQAGQLQDGEFEFDKPVGQGGAGRASDMPPTELRRGISLLEGEFGRADTQLSVLE
                     SLLFNRALDRNAMPSRDPIANSYITSGFGGRADPFGGGHQFHKGIDFEADVGDPVLAV
                     ADGVVSYSGVRSGYGNVVEVDHGNGYVTRYAHNSKLERQVGDLVRAGQEIAKAGSSGR
                     STGAHVHFEVWQDGAVVNPRKFLAVTSSGAHR"
     gene            4943..>5519
                     /gene="secA"
                     /locus_tag="PK399_11015"
     CDS             4943..>5519
                     /gene="secA"
                     /locus_tag="PK399_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112773.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPW13691.1"
                     /translation="MLNNLLTSVFGSRNERLLKQLSGIVKKINALEPQMQALSDDALK
                     AKTEEFKARSAKGESLDKLLPEAFAVCREASVRVFGMRHFDVQLIGGMVLHGGKIAEM
                     RTGEGKTLTATLAVYLNALEGKGVHVVTVNDYLARRDAAQMGKLYNWLGLSVGVVFPG
                     MPHSDKGAAYGCDITYGTNNEFGFDYLRDNMA"
BASE COUNT          946 a         1902 c         1858 g          813 t
ORIGIN      
        1 ggtcgacatg ggcgcgggca ccaccgacat cgcggtgttc gtgcagggcg cgatcagcca
       61 cacggccagc ctgccggtcg ccggcgacaa ggtgaccgag gacattgcgc acatgctgcg
      121 cacgcccacg ccggaagccg agcagatcaa ggtccgctac gcctgcgcgc tggcgcaact
      181 cgcgcgtgcc gaggaaagca tccaggtgcc cagcgtcggc gaccgcccgc cgcggcggat
      241 gccgcgccat gcgctggcgc aggcggtgca ggcgcgctac gaggaaatct tcgagatggt
      301 gcaggcggaa ctgcgccgca gcggcttcga gcaactggtg cgtgcaggca tggtcctgac
      361 cggcggcgcg tcgaagatgg aaggcgtggt cgaactggcc gaggagatgc tgcagatgcc
      421 ggtgcgcatc ggcatccccc agcacgtcag cggcctgggc gaagtggtcg gcaacccggt
      481 gcatgccacc ggcgttggcc tgctgctgat gggcagccag atcgagaacc cgcgccggcc
      541 gcagctccgc gatggccgcg cgttcggctg gatggacaag ttgaagaact ggtatcgcgg
      601 ccagttctag cgatggggcg tcgccgcgag ggcgcggtgg cgcgaggaag gcggaaggca
      661 gggcggtccg ggcaaggcaa caacccaagc gatctcacac aagcacgaca taacaagagg
      721 acacggacat ggcgcagaca ttcgaactgg tcgagaagat ggcaccgaac gcggtgatca
      781 aggtcatcgg cgtgggcggc ggcggcggca acgcggtcgc gcacatggtc aacagcagcg
      841 tggacggggt ggagttcatc accgccaaca ccgactcgca ggcgatcaag aactgcggcg
      901 cgaagctgca gctgcagctg ggtggcaacg tcaccaaggg cctgggcgca ggcgccaacc
      961 cggaagtcgg ccgccaggcc gcactggaag accgcgacac catcatcgac gcgctgaccg
     1021 gcgccgacat ggtgttcatc accgctggca tgggtggcgg caccggtacc ggcgccgcgc
     1081 cggtcgtcgc gcaggtcgcc aaggagatgg gcatcctgac cgtcgccgtg gtcaccaagc
     1141 cgttcccgtt cgaaggccgc cgccgcatgc aggtggcgct gaagggcatc gaggagctga
     1201 gccagcactg cgactcgctg atcacgatcc ccaacgaaaa gctgatcacc gtgctcggcc
     1261 gcaacgcgac catgatccag gccttccgcg cggccaacga cgtgttgctc ggcgcggtgc
     1321 agggcatcgc cgacctgatc gtgcgcccgg gcctgatcaa cgtcgacttc gccgacgtcc
     1381 gcaccgtcat gtccgaaatg ggcctggcga tgatgggcag cggcagcgcg cgcggcgacg
     1441 accgtgccca ggccgcggcc gagggcgcga tccagaaccc gctgctggac gacgtcaacc
     1501 tgtctggcgc caacgggatc ctggtcaaca tcaccgccgg tccggacttc accatggccg
     1561 agttcgacga agtcggccgc accgtggagc agttcgcctc ggaagacgcc accgtggtca
     1621 tcggcaccgt gctggatccg gagatgcagg acgaagtgcg cgtcaccgtg gtcgccaccg
     1681 gcctcaaccg caataccgcc ggcggccgcc agccggcccc gcgcagcgaa gcccgctacg
     1741 acagcggctt cgacgccccg cgccgtccgc aggtgcagtt gatcaacacc cagggcaagc
     1801 gcgacggcac caccggcctg atgatcgatg aggtctccga tccgttcgtg cccggtgcca
     1861 acaccggctt cgtcggcggc ctgcgccgca acgtcgatgc gggtccggcc gtggccgaac
     1921 tgccgaagga tgactacctg gacatcccgg cgttcctgcg tcgccaggcg gattgagcga
     1981 gacccgttcg ccctgagctt gtcgaagggt gagcgggccg ttgtggttcg acaggctcac
     2041 cacgaacgac ggtgaggcaa gtccatgtcc cttttgcacg ccgggttgcc cgcccggcgt
     2101 gattccaggt cggtcggcgt catgccggcc ggcccttttc ccatcaccat cctgcgcgtg
     2161 gaacacaccc gtttttacgt tcaggttcag caacctgcgt gctaacctcc gccgatgctt
     2221 cgccaacgca ccctcaagaa cctgatccgc gccaccggcg tggggttgca tggcggcgag
     2281 aaggtcttcc tcaccctgcg cccggcgccg atcgacaccg gcatcgtgtt ccgccgcatc
     2341 gacctggatc ccgtggtcga gatccccgcc agcgccgcgc tggtcaccga aaccaccctc
     2401 tgcaccggcc tgagttgcga aggcgccaag gtgcagaccg tcgagcacct gatgtcggcc
     2461 ctggccggcc tgggcatcga caacatctac atcgaactga ccgcgcccga agtgccgatc
     2521 atggatggct cggcgggccc gttcgtgttc ctgctgcagt ccgccggcat cgccgagcag
     2581 aacgcgccca agcggttcct gcgaatccgc aagcacgtcg aggtgcgcga cggcgacaag
     2641 gtcgccagct tcagcccgca tgacggcttc cgcgtcggct tcacggtcaa gttcaaccat
     2701 ccggcgatcc cggacacgct gtcgcgcgcc gaagtcgact tctcgaccag cgcctacgtc
     2761 agcgaggtga gccgcgcgcg caccttcggc ttcatgcagg acctggagta catgcgcgag
     2821 cgcaacctgg gcctgggcgg ttccatggac aacgcgatcg tgctcgacga gttccgcgtc
     2881 ctcaatgagg acggcctgcg ctacgccgac gaattcgtcc gccacaagat cctcgacgcg
     2941 gtcggcgacc tgtacctggc cggccgtccg atcatcggcg cctacgaggg ctacaagtcc
     3001 gggcacgccc tcaacaacag gctggtccgg gccctgctgg ccgatgccga ggcatgggaa
     3061 gaggtcagtt tcaccgaccc ggtggcagcg ccgccggtcg tttatggcga cgccatcccg
     3121 gcgacctgaa cgcgttcgcc tgcgtcccgg ggctgtgacc gggtgcacgc attcagctga
     3181 atcgttagtc tttatttaac aaaatcacgg acttgtgacg ttaggatgcc cacccggccg
     3241 ggttgggtgc gttcctacgt cccccgccgc ccgggtttgc ggtttccccc agcgcgctgt
     3301 tccgacacgt cgggcgcgtc agaccgcaac agtgccaagg cagccgccag tcccgactgg
     3361 ccggccgccg acattggtgg agcggttgtc gcggctggcg ggatcggtcg caacggttgg
     3421 gtcgtcgtcc tgacggtcaa ccccgccaca tcgagcccga cggatcgggc agcgtcgacc
     3481 agttcctgcg tggccaaccg caatttggca tgccacaccg ggccatcgac gacgaaaacg
     3541 agcttgtcgc cacgaacgtt cgccagccgc gcatgcgcgg ccagaacggg cggcaggtgg
     3601 ggacgcaaca actggtccac cgcgtcgagc cacagtgccc ggcgcagggt gccgccggag
     3661 gatccgtcga gcagcgcttg cagcgccgcg cgcggacccg aaggaggcgt cgcccccgct
     3721 gggggcgtgg accgagaacc aggcataccc gatgaccgaa acaagcaagt cacacgatac
     3781 ccgccggcgc gtgatcgcgc aggcacaggc cttccgcttc tgggcgatgc gccgtccgtt
     3841 ggccgccctt ggcgtgctgc tcggtgccgg cctcttgctc ggcgccggtg ggcgcagcgt
     3901 ggtgggcatg gccgaggtcg aagccatgca ggccgccgag gcgaatcggc aggccgagct
     3961 ggccaaggtc cgccgcgacg cccagcgcga ggtcaatgca ctcgccgccc gcctggccga
     4021 gttgcaggcg caggcgaacc gcctcaacgc cctcggcgcg cgcctgaccc aggccggcca
     4081 gctgcaggac ggcgaattcg agttcgacaa gccggtcggc cagggtggcg ccggccgcgc
     4141 cagcgacatg ccgccgaccg agctgcgccg tgggatttcg ctgctggaag gcgagttcgg
     4201 ccgtgccgac acccagttgt cggtgctgga gagcctgctg ttcaaccgcg ccctggatcg
     4261 caacgcaatg ccgtcgcgcg acccgatcgc caacagctac atcacctccg gcttcggcgg
     4321 tcgcgccgat ccgttcggcg gcggccacca attccacaag ggcatcgact tcgaagccga
     4381 cgtcggcgac ccggtgctgg ccgtggccga tggcgtggtc agctactccg gtgtgcgatc
     4441 cggctacggc aacgtggtcg aggtcgacca cggcaacggc tacgtgaccc gctacgcgca
     4501 caactccaag ctggaacggc aggtcggcga cctggtgcgt gcgggccagg aaatcgccaa
     4561 ggccggctcc agtggtcgct cgaccggtgc ccacgtgcac ttcgaggtct ggcaggacgg
     4621 cgcggtggtg aacccgcgca agttcctcgc ggtgacgtcc agcggcgcgc accgctaagg
     4681 ccgcttcccg tccgtccagc cgcgttgcgg catggacgcg cggggagggg gcatcgcctg
     4741 tgtgccccgt cccggcaccc cgcttgaagc ggggtggacg ccggcttgat aacgcctgcc
     4801 ggtgcctcca gctacactag cggactgcga aacgggcgca atgcgcccgt ttttgtcgtt
     4861 tcgccgctga tacgccatcc ccgatggccc accgatgccg ccgcgccccg cgcgcgcatc
     4921 cccgacccgg attcgtcgcc ccatgctcaa caacctgctc accagcgtct tcggcagccg
     4981 caatgaacgc ctgctcaagc aactgtccgg gatcgtcaag aagatcaacg cgctggaacc
     5041 gcagatgcag gcgctgtccg acgacgcgct gaaggcgaag accgaggagt tcaaggcgcg
     5101 cagcgccaag ggcgagtcgc tggacaagct gctgccggaa gcgttcgcgg tctgccgcga
     5161 agcctcggtg cgggtgttcg gcatgcgcca cttcgatgtc cagctgatcg gcggcatggt
     5221 cctgcacggc ggcaagatcg ccgaaatgcg cactggtgag ggcaagaccc tgaccgcgac
     5281 cttggcggtg tacctcaacg cgctggaagg caagggcgtg cacgtggtca ccgtcaacga
     5341 ctacctggcc cgccgcgacg ccgcgcagat gggcaagctg tacaactggc tgggcctgag
     5401 cgtgggcgtg gtgttccccg gcatgccgca ttccgacaag ggcgccgcct acggctgcga
     5461 cattacctac ggcaccaaca acgaattcgg cttcgactac ctgcgcgaca acatggcgc
//
LOCUS       DAOOCX010000151         5500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_13944_length_5500_cov_9.734803, whole genome shotgun sequence.
ACCESSION   DAOOCX010000151 DAOOCX010000000
VERSION     DAOOCX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13944_length_5500_cov_9.734803"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..580
                     /locus_tag="PK399_11020"
     CDS             2..580
                     /locus_tag="PK399_11020"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPW13692.1"
                     /translation="MLLMIDNYDSFTYNLVQYLQSLGAEVRVERNDALSVDEIERLAP
                     QRIVISPGPCTPNEAGVSMGVIERLGATTPILGVCLGHQSMGQVYGGDVIRAGRIMHG
                     KTSRIRHEGKGVFAGLPDGYEATRYHSLVVDKTTLPDCMEITAWTDNEDGSMEEIMGL
                     RHREFPVEGVQFHPESILTQHGHALLKNFLER"
     gene            582..1616
                     /gene="trpD"
                     /locus_tag="PK399_11025"
     CDS             582..1616
                     /gene="trpD"
                     /locus_tag="PK399_11025"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPW13693.1"
                     /translation="MTFTPQHALQRAIEHREIFFDEMVELMRQVMRGEVSPAMTAAIL
                     TGLRVKKETVGEIAAAATVLREFARPVDVADRTGLVDIVGTGGDGAHTFNISTASMFV
                     VAAAGAKVAKHGNRSVSSKSGSADVLEALGANIELQPEQVSACIARCGIGFMFAPVHH
                     PAMKVVAPVRKEMGVRTLFNILGPLTNPAGAPNILMGVFHPDLVGIQVRVLQELGAQR
                     ALVVWGRDGMDELSLGSATLVGELRDGKVREYELHPEDFGIPMAHSRNLKVADAEQSM
                     AMLLQALDNHEGLPRQIVAYNAGAALYAAGVAEDIGDGIARARKAIASGAARAKLDEF
                     VATTQALAGA"
     gene            1648..2835
                     /gene="iadA"
                     /locus_tag="PK399_11030"
     CDS             1648..2835
                     /gene="iadA"
                     /locus_tag="PK399_11030"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HPW13694.1"
                     /translation="MIQTLTATPMRLLRNADVYAPEALGRRHLLLGGGKVLWMGEVDP
                     ALPASLDVDAIDLDGARLVPGFIDGHVHVSGGGGEAGFATRVPAPLLSRYTTAGVTSV
                     VGLLGTDDATRNTAELLSHVYALREQGLSAWGYCGGYHVPPATLTGSLRSDIALVEPL
                     IGIGELAISDHRSSQPTLDELLRLASEAHVAGLMSGKAGIVHLHLGDGVRGLDLVRRA
                     LDTSELPPRVFQPTHVNRKQALFEEALELARRGCHVDITAFPVEEGEDAWPADEALVR
                     YLDSGAPPERVSISSDAGGCLPCFDGEGRVCGMDVGHSGSMLDTLNALLARGLPLERA
                     LPAFTSNVAGLLRLAGKGRIAVGCDADLVALDAAGRADLVLARGAVHVRAGVPVRRGM
                     FER"
     gene            2860..3702
                     /locus_tag="PK399_11035"
     CDS             2860..3702
                     /locus_tag="PK399_11035"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HPW13695.1"
                     /translation="MCPSKVADGETRGWIVPIGGAEDKESRRRILKRFVQLCGGRDAR
                     IAVIPTASKLADTGERYEGLFDRLEAGRVDTLDFESRADGGREDFLEILRQCTGVFIT
                     GGNQLRLSTILGGTAATKLIRERNADGVPVGGTSAGASILAEHMIAFGSEGGSPRADS
                     VNLAPGFGLTNRFIIDQHFRQRDRLGRLTAALAYNPFAIGIGLDEDTAAFIGPDNTLE
                     VEGSGAITVVDASQVQFTSMDQVGGSDAVCIIGLQLHILTAGATFNLHTRQASPGELL
                     ARKD"
     gene            3736..>5500
                     /locus_tag="PK399_11040"
     CDS             3736..>5500
                     /locus_tag="PK399_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPW13696.1"
                     /translation="MRILNRNVYVGPSQYAKFPVIRLELDLGALEQWPTAKLGDGFVD
                     GLLAALPGLAEHGCSYREPGGFVRRMREGEGTWLGHVLEHVAIELQNVAGEDVTFGKT
                     RSMSDDRPGVYSVVYEYAQKEEGIAAGELALKLLDSLLPAGLRSATDDSWHWEEERDG
                     FIRYAQRRALGPSTMSLVRAAEERGIPWLRLNEQSLVQLGHGKYQQRIQATVTGRTPH
                     IAVELASDKEETNKILGSLGLPVPRQVLVTSQTDAIKAARRLGGPVVLKPYNGNHGRG
                     ITINVSAEDDIRAGFEAAREHSRSVIVETYLAGDDHRLLVVNGELVAATRRTPGHVVG
                     DGASTVAQLVEVVNSDPRRGVGHEKVLTKLKLDREAELMLERAGYTADSVPKAGEVVP
                     LRSTANLSTGGTATDVTDIIHPDNRAMAERAVRAIGLDVGGVDFLSTNIAESYKAIGG
                     GICEVNAAPGFRMHIAPSEGTPRDAAGPVIDMLFPPGTPSRVPIAAVTGTNGKTTTSR
                     MLAHIAKMAGYTPGLTTTDGVYIDGQRTVEGDMTGPVSARMVMADPQIDFAVLETARG
                     GLVRAGMGVAEVDVGAVINVAS"
BASE COUNT          900 a         1857 c         1930 g          813 t
ORIGIN      
        1 catgctgctg atgatcgaca actacgacag cttcacctac aacctcgtgc agtacctgca
       61 gtcgctgggc gccgaggtgc gggtggagcg caacgatgcg ttgagcgtcg atgaaatcga
      121 acgactcgca ccgcaacgca tcgtcatctc gccggggccg tgcacgccga acgaggctgg
      181 cgtctcgatg ggcgtgatcg agcggctggg cgcgaccacg ccgatcctcg gcgtctgcct
      241 gggccaccag agcatgggcc aggtctacgg cggcgacgtg atccgcgcgg gccgcatcat
      301 gcatggcaag acctcgcgca tccgccatga aggcaagggc gtgttcgccg gcctgccgga
      361 cggctatgag gccacgcgct atcactcgct ggtggtcgac aagacgacgc tgcccgattg
      421 catggaaatc actgcctgga ccgacaatga ggacgggtcg atggaggaga tcatgggcct
      481 gcgccaccgt gaattcccgg tggaaggggt gcagttccat ccggagtcga tcctgaccca
      541 gcatggccat gcgctgctga agaatttcct ggaacgctga aatgaccttc accccgcaac
      601 acgccctgca acgcgcgatc gagcaccgcg agatcttctt cgacgaaatg gtcgagctga
      661 tgcgccaggt gatgcgtggc gaggtttccc cggcgatgac cgcggcgatc ctcaccggcc
      721 tgcgggtgaa gaaggaaacc gtgggcgaga tcgccgcggc ggccacggtg ctgcgcgaat
      781 tcgcgcgtcc ggtcgatgtc gccgatcgca ccggcctggt cgacatcgtc ggcaccggcg
      841 gcgatggcgc gcacacgttc aacatctcca ccgcgtcgat gttcgtggtc gccgcggccg
      901 gcgccaaggt cgccaagcac ggcaaccgca gcgtgtcgtc gaagtccggc agcgccgacg
      961 tgctggaagc gctgggcgcc aacatcgaat tgcagccgga acaggtctcg gcctgcatcg
     1021 cgcgctgcgg gatcggcttc atgttcgcgc cggtgcacca tccggcgatg aaggtggtgg
     1081 caccggtgcg caaggaaatg ggcgtgcgca cgctgttcaa catcctcggc ccgctgacca
     1141 acccggccgg cgcgccgaac atcctgatgg gcgtgttcca tccggacctg gtcggcatcc
     1201 aggtgcgcgt tctgcaggaa ctcggcgcgc agcgcgcact ggtggtctgg ggccgcgacg
     1261 gcatggacga actgtcgctg ggctccgcca ccctggtcgg cgaattgcgc gacggcaagg
     1321 tgcgcgagta cgaattgcat cccgaggact tcggcatccc gatggcgcac agccgcaacc
     1381 tcaaggtcgc ggacgccgag caatcgatgg cgatgctgct gcaggcgctg gacaaccacg
     1441 aaggcctgcc gcggcagatc gtggcctaca acgccggcgc ggcgctgtac gcggcgggcg
     1501 tggccgagga tatcggcgac ggcatcgcgc gcgcgcgcaa ggcgatcgcg tcgggcgcgg
     1561 cgcgggccaa gctggacgag ttcgtggcga ccacgcaggc gcttgccggc gcttgacgct
     1621 gcatcgcgcg ccgcgcaggc tgccgcgatg atccagaccc ttactgccac cccgatgcgg
     1681 ctgctccgca atgccgacgt gtatgcgccc gaagcgctgg gccggcgcca cctgctgctc
     1741 ggcggcggca aggtgttgtg gatgggcgag gtcgatccgg cattgccggc atcgctcgat
     1801 gtcgacgcca ttgatctcga tggcgcgcgc ctggtccccg gcttcatcga cggccatgtg
     1861 cacgtgagtg gcggcggcgg cgaggccggc ttcgccaccc gcgtgccggc gccattgctc
     1921 agtcggtaca cgaccgctgg cgtcaccagt gtggtcggcc tgctcggcac cgacgacgcc
     1981 acccgcaaca ccgcggaact gctctcgcat gtctatgcat tgcgcgagca gggcctgtcg
     2041 gcatggggct attgcggcgg ctaccacgtg ccgccggcca cgctcaccgg cagcctgcgc
     2101 agcgacatcg ccctggtcga gcccttgatc ggcatcggcg aactggcgat cagcgaccat
     2161 cgttccagcc agccgaccct cgacgaactg ctgcggctcg cctccgaggc gcatgtggcc
     2221 ggcctgatga gcggcaaggc cggcatcgtc cacctgcacc tgggcgacgg cgtgcgcggc
     2281 ctcgacctgg tgcgccgggc gctggatacc agcgaattgc cgccgcgggt cttccagccg
     2341 actcatgtga atcgcaagca ggcgctgttc gaggaagccc tcgagctggc gaggcgcggt
     2401 tgccatgtcg acatcaccgc gttcccggtc gaggagggcg aagacgcttg gccggccgac
     2461 gaggcgttgg tccgctacct cgacagcggc gcgccgccgg agcgggtcag catcagctcc
     2521 gacgccggcg gctgcctgcc ctgcttcgac ggcgaggggc gcgtctgcgg gatggacgtg
     2581 ggccattccg gctcgatgct ggacaccttg aacgcgctgc tggcgcgcgg cctgccgctg
     2641 gaacgtgcgc tgccggcctt caccagcaac gtggccgggc tgctgcggct ggccggcaag
     2701 ggccggatcg cggtcggctg cgatgccgac ctggtggcgc tggatgccgc cggtcgcgcc
     2761 gacctggtcc tggcccgcgg ggccgtgcat gtgcgggcgg gcgtgccggt gcggcgtggt
     2821 atgttcgaac gctgaatacg tcaggggaat gcgccgtaca tgtgtcccag caaggtagcc
     2881 gacggcgaaa cgcgcggctg gatcgtcccc atcggggggg cggaagacaa ggaaagccgg
     2941 cgccgcatcc tcaagcgctt cgtgcagttg tgtggcggcc gtgacgcgcg catcgcggtg
     3001 atccccaccg ccagcaagct ggccgatacc ggcgaacgct atgagggatt gttcgatcgg
     3061 ctcgaggccg ggcgggtgga cacgctcgat ttcgagagcc gtgccgatgg cgggcgcgag
     3121 gacttcctcg agatcctgcg ccagtgcacc ggggtgttca tcaccggcgg caaccagctg
     3181 cgcctgtcca ccatcctcgg tggcaccgcc gcgaccaagc tgatccgcga gcgcaacgcc
     3241 gacggcgtgc cggtgggcgg caccagcgcc ggcgccagca tcctcgccga gcacatgatc
     3301 gcgttcggta gcgagggcgg ttcgccgcgc gcagacagcg tgaatctggc gcccggcttc
     3361 gggctgacca accgcttcat catcgaccag catttccgcc agcgcgaccg gttgggccgg
     3421 ctgaccgcgg cgttggccta caacccgttc gccatcggca tcggcctgga cgaggacacc
     3481 gcggccttca tcggcccgga caacacgctg gaggtcgaag gcagcggcgc gatcaccgtg
     3541 gtcgatgcca gccaggtgca gttcacttcg atggaccagg tgggcggcag cgatgcggtc
     3601 tgcatcatcg gcctgcaact gcatatcctg accgcgggcg cgaccttcaa cctgcacacg
     3661 cgccaggcct cgccgggcga actgctcgcg cggaaggact gactcatcac gcaacaccac
     3721 cgggcggagg gctcgatgcg gatcctcaat cgcaatgtct acgtggggcc ttcgcagtac
     3781 gcgaagttcc cggtcatccg ccttgaactg gacctcggcg cgctggagca atggcccacc
     3841 gcgaagctgg gcgatgggtt cgtcgacggc ctgctggcgg cgttgcccgg actggccgag
     3901 cacggctgct cctatcgcga gcccggcggc ttcgtccgcc gcatgcgcga gggcgagggc
     3961 acctggctgg gccatgtgct ggaacacgtc gccatcgaac tgcagaacgt cgccggcgag
     4021 gatgtgacct tcggcaagac ccgcagcatg tccgatgacc gccccggcgt gtattcggtg
     4081 gtctacgaat atgcgcagaa ggaagaaggc atcgccgccg gcgagctggc gctgaagctg
     4141 ctggactcgc tgctgcccgc aggcctgcgc agcgccaccg acgacagctg gcactgggaa
     4201 gaagaacgcg acggcttcat ccgctacgcg cagcgccgcg cgctgggtcc gtcgacgatg
     4261 tcgctggtgc gcgccgccga ggaacgcggc atcccgtggc tgcgcctcaa cgagcaatcg
     4321 ctggtgcagc tgggccacgg caagtaccag caacgcatcc aggccacggt caccgggcgc
     4381 accccgcaca tcgcggtgga actggcgagc gacaaggaag agaccaacaa gatcctgggc
     4441 tcgctgggcc tgccggtacc ccggcaggtg ctggtgacca gccagaccga tgcgatcaag
     4501 gccgcgcgca ggctgggcgg cccggtagtg ctcaagccgt acaacggcaa ccacggccgc
     4561 ggcatcacca tcaacgtcag cgcggaagac gacatccgcg ccggcttcga ggccgcgcgc
     4621 gagcattcgc gatcggtgat cgtggaaacc tacctggccg gcgacgacca tcgcctgctg
     4681 gtggtcaacg gcgagctggt cgcggcgacg cggcgcacgc ccggccacgt ggtcggtgat
     4741 ggtgcatcga cggtggcgca actggtcgag gtggtgaaca gcgatccacg ccgcggcgtg
     4801 ggccacgaga aagtgctgac caagctcaag ctcgaccgcg aggccgaact gatgctggag
     4861 cgcgcgggct acaccgcgga ctcggtgccg aaggccggcg aagtggtgcc gctgcgttcc
     4921 accgcgaacc tgtccaccgg cggcaccgcc accgatgtca ccgacatcat ccatcccgac
     4981 aaccgcgcga tggcggaacg cgcggtgcgc gcgatcgggc tggacgtggg cggcgtggat
     5041 ttcctcagca ccaacatcgc ggagagctac aaggcgatcg gcggcggcat ctgcgaggtc
     5101 aatgccgcgc ccggtttccg catgcacatc gcgcccagcg aaggcacgcc gcgcgatgcc
     5161 gccgggccgg tcatcgacat gctgttcccg cccggcacgc cgagccgcgt gccgatcgcc
     5221 gcggtgaccg gcaccaacgg caagaccacc acctcgcgca tgctcgcgca catcgccaag
     5281 atggccggct acacgccggg cctgaccacc accgacggcg tctacatcga cggccagcgc
     5341 acggtggagg gcgacatgac cgggccggtg tccgcgcgca tggtgatggc ggatccgcag
     5401 atcgatttcg cggtgctgga aaccgcgcgc ggcggcctgg tccgtgccgg catgggcgtg
     5461 gccgaggtcg acgtgggcgc ggtgatcaac gtcgcctccg
//
LOCUS       DAOOCX010000152         5428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_14153_length_5428_cov_7.986414, whole genome shotgun sequence.
ACCESSION   DAOOCX010000152 DAOOCX010000000
VERSION     DAOOCX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14153_length_5428_cov_7.986414"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PK399_11045"
     CDS             <1..316
                     /locus_tag="PK399_11045"
                     /inference="COORDINATES: protein motif:HMM:NF017457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agenet domain-containing protein"
                     /protein_id="HPW13697.1"
                     /translation="AQSRGDWVLGQWQGGAYWFPGVVQERNGDSVTILYDDGTRETVP
                     SKRVRPYTWALGTRVECRWSGGSEWYTGKITGMAKDGNEIDVLYDDGDRERIATGGCR
                     SR"
     gene            324..1121
                     /locus_tag="PK399_11050"
     CDS             324..1121
                     /locus_tag="PK399_11050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010374496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPW13698.1"
                     /translation="MQSLLRLLQAPRSPSKSPAVQRDSVGFVLDDGRHVDVLRVRDPR
                     ARRIKLSVDERGARLSLPLRASLVAGERFLHEHRHWLAVQLAAQGEHAGEGLLRGVTD
                     ALPLRGLDVPLRWTGGRAMRIDIDPDAGLLFAAPVTAGDAGLRRALRDFYEAQGRADV
                     GRWLPGYLEGLPRAPRRIVFKRTSTQWGSLAPDGTLSLDLALVLARPSAFEYVLVHEL
                     CHLLHADHSRRFWREVEARFPTWRDERHYFHAEGRRLKARLRGLLAG"
     gene            1169..1684
                     /locus_tag="PK399_11055"
     CDS             1169..1684
                     /locus_tag="PK399_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13699.1"
                     /translation="MTHPAIRLSIAVAVAALLVAPGCKKAQDAALETAIEGATGNKVD
                     VQKDGNEMTIKTEQGDLRIATAEDGGSVAMPANFPKDVFLPAENTLASAMDMGGMQML
                     NMTTPQELTAVSADIEKSMQGQGWKREMAMQAGGGNTLMYSKDKRQVVYQMLKADEGG
                     TQLAIRTGEDG"
     gene            complement(1713..2585)
                     /locus_tag="PK399_11060"
     CDS             complement(1713..2585)
                     /locus_tag="PK399_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014505179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW13700.1"
                     /translation="MRDIEIEIPLGRIGALRSGEPGAPKVLALHGWLDNAASFVPLSA
                     HLHGIDLVAIDQPGHGRSAHLPPGSDYSFVGAMNAVLDVADTLGWERFALLGHSMGAG
                     IASMLAASCPQRIGRLVAIEALGALAEAPERTVARMRDAVVAHRRIKDKRLRMFADID
                     SAVRTRQFASLVPGSGLDEASVRLLVERGLREVRVDGESVGYEWSSDPRLTLPTMVRM
                     TEPQVEDLVAGIECPTLAIFADPAQPYLPDALRRRRVGLLPNGELKVIAGGHHLHMQQ
                     AADVAAAIGDFLQR"
     gene            complement(2585..3553)
                     /gene="hemH"
                     /locus_tag="PK399_11065"
     CDS             complement(2585..3553)
                     /gene="hemH"
                     /locus_tag="PK399_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HPW13701.1"
                     /translation="MNPASESPPALLLVNLGTPAAPTAPAVRRYLAEFLHDHRVVQLS
                     RWLWCPLLHFVILPLRSPKVAVKYASVWLPGGSPLAVHTAALADAVRERLPGRRVLHA
                     MRYGEPSIAAALDVLRAQGVRSVRVLPLYPQYSTTTTASVADAIAKHARGLQVAMLHD
                     YHRDPGWAAAVADSIRTHWATHGRGERLLLSFHGIPQRLVDGGDPYALQCEASTVAIA
                     EALGIPRGDILLTFQSRFGKERWLQPYTQQALEALGREGVRTIDVACPGFAVDCLETL
                     EEIAVENAHAFAAAGGETLRYIPCLNAGDAHAEALAALVQGDEGWS"
     assembly_gap    3602..3701
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3779..4606
                     /locus_tag="PK399_11070"
     CDS             3779..4606
                     /locus_tag="PK399_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HPW13702.1"
                     /translation="MPHAPRRLALALALAVATSGALAGEREDVRAGEAVRVVEQIQAI
                     PESAIPDRLLDEAKAIVVVPDTIKAGFVFGGRRGLGLMSVKTPQGTWSNPVFVKLAGA
                     SFGLQAGVQSADVILVFRSERGLDSIVNGKVTLGADAGVAAGPVGRNAAAATDGQLKA
                     EIWSWSRARGLFAGIALDGAVLSIDNGANRSTYGNAVTPRAVFEGRAPQAPSAAIVAF
                     RDALEEATASARQARGGTPAPVAAPAEPGAASVQPLYNDNPATTTPLDAAPAQPVKP"
     gene            4655..4876
                     /gene="tatA"
                     /locus_tag="PK399_11075"
     CDS             4655..4876
                     /gene="tatA"
                     /locus_tag="PK399_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HPW13703.1"
                     /translation="MGSFSIWHWLIVLVIVLLVFGTKRLTSGARDIGKAVDEFKKGMR
                     GDDDKPAARLGGDKTPSESEQRDRDDVAR"
     gene            4895..5131
                     /gene="tatB"
                     /locus_tag="PK399_11080"
                     /pseudo
     CDS             4895..5131
                     /gene="tatB"
                     /locus_tag="PK399_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
     gene            5232..>5428
                     /locus_tag="PK399_11085"
     CDS             5232..>5428
                     /locus_tag="PK399_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPW13704.1"
                     /translation="MTDAADDSERPLIAHLLELRSRLLRGVVGLVVVLLGLLPFANKL
                     YALLAQPLLDKLPKGGQLIAT"
BASE COUNT          835 a         1889 c         1864 g          740 t
ORIGIN      
        1 ggcgcagtcg cgcggcgact gggtactcgg gcaatggcag ggcggcgcgt actggttccc
       61 gggcgtggtc caggaacgca atggcgacag cgtcaccatc ctctacgacg atggcacccg
      121 cgaaacggtg ccgagcaagc gcgtgcgccc ctacacctgg gcgctgggca cccgcgtgga
      181 atgccggtgg tccggtggca gcgagtggta caccggcaag atcaccggca tggcgaagga
      241 cggcaacgag atcgacgtgc tctacgacga cggcgaccgt gagcgcatcg ccaccggcgg
      301 ctgccgctcc cgctaggaca cgcatgcagt ctttgctgcg cctgctgcag gcgccacgca
      361 gtccctccaa gtctcccgcc gtccagcgcg acagcgtcgg cttcgtgctg gatgacggcc
      421 gccatgtgga cgtcctgcgc gtgcgcgacc cgcgcgcgcg ccggatcaag ctgtccgtcg
      481 atgagcgcgg cgcgcgcttg agcctgccgc tgcgggcgag cctggtcgcc ggggaacgct
      541 tcctgcacga acaccgccat tggctggccg tgcagttggc ggcgcagggc gaacacgccg
      601 gcgaagggtt gctgcgtggc gtcaccgatg cgctgccgtt gcgcgggctg gacgtgccgt
      661 tgcgctggac cggcggccgg gcgatgcgga tcgacatcga ccccgacgcc ggcctgctgt
      721 tcgccgcacc ggtgacggca ggcgatgccg gcctgcgccg cgcgctgcgc gatttctacg
      781 aagcgcaggg ccgtgccgac gtgggccgct ggttgccggg ttacctcgaa ggactcccac
      841 gcgcgccgcg gcggatcgtg ttcaagcgca cctcgacgca atggggctcg ctggcaccgg
      901 acggcacgtt gtcgctggac ctggcgctgg tactggcgcg gccgtccgcg ttcgagtacg
      961 tgctggtcca cgagctctgc cacctgctcc acgccgacca ctcgcggcgc ttctggcgcg
     1021 aggtggaagc gcgcttcccg acctggcggg acgaacgcca ttacttccac gccgagggcc
     1081 gccgcctgaa ggcgcgactg cgcggcctgc tggccggttg acccgccagc ctgcgttgcc
     1141 tccggcagga caacgaagga attccgccat gacccacccc gccatccgcc tgtcgatcgc
     1201 cgtcgcagtc gccgccctcc tggtcgcgcc cggctgcaag aaggcgcagg acgccgcact
     1261 cgagaccgcg atcgaaggcg ccaccggcaa caaggtggac gtccagaagg acggcaacga
     1321 aatgaccatc aagactgagc agggcgacct caggattgcg acggccgagg atggcggcag
     1381 cgtggcgatg ccggccaatt tccccaagga cgtattcctg ccggccgaga acaccctcgc
     1441 cagcgcgatg gacatgggcg gcatgcagat gctcaacatg accaccccgc aggaattgac
     1501 cgccgtctcc gccgacatcg agaagtcgat gcagggccag ggctggaagc gcgagatggc
     1561 gatgcaggcc ggcggcggca ataccctgat gtacagcaag gacaagcgcc aggtcgtcta
     1621 ccagatgctc aaggcagatg agggcggcac ccaactggcg atccgcaccg gcgaggacgg
     1681 ctgagccagc ccgccgatac gcggccggcg gctcaacgct gcaggaaatc gccgatcgcc
     1741 gccgctacgt cggccgcttg ctgcatgtgc aggtggtggc cgccggcgat gaccttcaat
     1801 tcgccgttcg gcagcaggcc aacccggcgg cggcgtagcg cgtcgggcag gtagggttgc
     1861 gcggggtcgg cgaagatcgc cagcgtcggg cattcgatcc ccgccaccag gtcttcgacc
     1921 tgcggttcgg tcatccgcac catggtcggc agggtcagcc gcggatcgct gctccactcg
     1981 tagcccacgg attcgccatc gacgcgaacc tcgcgcaggc cgcgttcgac cagcagccgc
     2041 accgaggcct cgtccagccc gctgcccggc accaggctgg cgaactgccg cgtgcgcacc
     2101 gcactgtcga tgtcggcaaa catgcgcagg cgcttgtcct tgatcctgcg gtgggcgacc
     2161 accgcgtcgc gcatgcgcgc caccgtgcgc tccggggctt cggccagcgc gcccagcgcc
     2221 tcgatcgcga ccagccgccc gatccgttgc gggcaggacg cggccagcat gctggcgatg
     2281 ccggcaccca tcgaatgccc gagcagggcg aagcgctccc agcccagcgt atcggcgaca
     2341 tcgagcaccg cgttcatcgc accgacgaag gaatagtcgc tgccgggcgg caggtgcgcg
     2401 ctgcggccgt ggccgggctg gtcgatcgcc accaggtcga tgccatgcag gtgcgccgac
     2461 agcggaacga agctcgccgc gttgtccagc cagccgtgca gggccagcac cttcggcgcg
     2521 cccggttcgc cgctgcgcaa ggcgccgatg cgaccgagcg ggatctcgat ctcgatgtcg
     2581 cgcatcacga ccagccctcg tcgccttgca ccagcgccgc caatgcttcc gcatgcgcat
     2641 cgcccgcatt gaggcacggg atgtagcgca gggtttcgcc gccggccgcg gcgaacgcat
     2701 gcgcgttctc caccgcgatt tcctccagcg tttccaggca atccaccgca aaaccggggc
     2761 aggcgacgtc gatcgtgcgc acgccctcgc gccccagcgc ttccagcgcc tgctgcgtgt
     2821 acggctgcaa ccagcgttcc ttgccgaagc gcgactggaa cgtcagcagg atgtcgccgc
     2881 gtgggatgcc cagggcctcg gcgatggcga cggtgctggc ctcgcactgg agcgcgtagg
     2941 gatcgccgcc atcgaccagg cgctgcggga tgccgtggaa cgacagcagc aggcgctcgc
     3001 cgcggccgtg ggtcgcccag tgcgtgcgga tcgaatcggc gaccgccgcg gcccagccgg
     3061 gatcgcggtg gtagtcgtgg agcatcgcca cctgcaagcc cctggcatgc ttggcgatgg
     3121 catcggcgac gctggcggtc gtcgtggtcg agtactgcgg atacagcggc agcacccgca
     3181 ccgagcgcac gccctgcgca cgcagcacat ccagcgcagc ggcgatcgac ggttcgccgt
     3241 agcgcatcgc atgcagcacg cgccggccgg gcaagcgctc gcgcaccgca tcggccagcg
     3301 ccgcggtatg cactgccagc ggtgagccgc cgggcaacca gaccgaggca tatttcaccg
     3361 cgaccttcgg gctgcgcagc ggcaggatca cgaagtgcag caacgggcac cacagccagc
     3421 ggctgagctg gaccacgcgg tggtcgtgga ggaattcggc gaggtagcgg cgcaccgcgg
     3481 gcgcggtcgg cgcggcgggc gttcccaggt tgaccagcag cagcgccggc ggcgattcgg
     3541 aagcgggatt catcccgaca ggatgacatg cggaagcggc aaaccccgat ggcgatggcc
     3601 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggatgtccc ggcgcggttc
     3721 cttgtcggtt catcgcggcg cgcctagcct gaacgccccg ccaccggaag ccacgcccat
     3781 gccccatgct ccccgccgcc ttgccctcgc cctggccctg gcggtcgcta ccagcggggc
     3841 ccttgcaggc gaacgcgagg acgtgcgcgc cggcgaagcg gtgcgcgtgg tcgagcagat
     3901 ccaggccatc cccgaatcgg ccatccccga ccgcctgctc gacgaggcca aggcgatcgt
     3961 ggtcgtcccc gacaccatca aggccggctt cgtgttcggt ggccgccgtg gcctgggcct
     4021 gatgtcggtg aagacaccgc agggcacctg gtcgaacccg gtgttcgtga aactggcggg
     4081 tgccagcttc ggcctgcagg ccggcgtgca gtcggccgac gtgatcctgg tgttccgcag
     4141 cgagcgcggg ctggactcga tcgtcaacgg caaggtcacc ttgggcgccg atgccggcgt
     4201 ggcggccggg ccggtcgggc gcaatgccgc cgccgccacc gacggccagt tgaaagcgga
     4261 gatctggtcc tggtcgcgcg cccgcgggtt gttcgccggg atcgccctgg acggcgcggt
     4321 gctgtcgatc gacaacggcg ccaaccgcag cacctacggc aatgccgtga ccccgcgcgc
     4381 ggtgttcgag ggccgtgcgc cgcaggcacc gtccgcggcc atcgtggcgt tccgcgatgc
     4441 gctggaggaa gccacggcct cggcacgcca ggcgcgcggc ggcaccccgg ccccggttgc
     4501 cgcgccggcg gaaccgggcg cggccagcgt gcaaccgctg tacaacgaca acccggccac
     4561 caccacgcca cttgacgccg cgccggcgca gccggtcaag ccctgaggcg atcggcgtta
     4621 gaatcgcagc cccctccctc cacaaaggca catcatgggc agcttcagca tctggcactg
     4681 gctgatcgtg ttggtgatcg tgctgctggt gttcggcacc aagcgcctga cctcgggcgc
     4741 gcgcgacatc ggcaaggcgg tggacgagtt caagaaaggc atgcgcggtg atgacgacaa
     4801 gccggccgcg cggctcggcg gcgacaagac cccgtccgag agcgagcaac gcgaccgcga
     4861 cgacgtcgcc cgctaagccg cgccgcggca tccgatgttc gatttttcct tcggcgagtt
     4921 gatggtcgtc gccttggtgg cgctgatcgt gctcggcccc gagcgcctgc ccaaggcggc
     4981 acgcttcacc ggcctgtggg tgcggcgcgc acgcgcgcag tggtattcgg tgaagtcgga
     5041 actggagcag gaactggcca gcgacgaact caagcgcagc ctgcatgccg ggcgtgacgc
     5101 catgcgcgag gcggaaaccc acttgcgcga cttcgccagc gacatcgaac gccccgcccc
     5161 ggcaccggac gcggcggcgg ataccgcgcc ccgcacggat gaaccggccg cgcccacgga
     5221 accgcgccgc gatgactgat gccgccgacg acagcgaacg cccactgatc gcgcacctgc
     5281 tggaactgcg gtcgcggctg ctgcgcgggg tggtcggcct ggtcgtggtg ctgctcggcc
     5341 tgttgccgtt cgccaacaag ttgtacgcgc tgctggcgca gccgctgctg gacaagctgc
     5401 ccaagggcgg ccagctgatc gccaccca
//
LOCUS       DAOOCX010000153         5384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_14312_length_5384_cov_4.125727, whole genome shotgun sequence.
ACCESSION   DAOOCX010000153 DAOOCX010000000
VERSION     DAOOCX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14312_length_5384_cov_4.125727"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PK399_11090"
     CDS             <1..323
                     /locus_tag="PK399_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPW13705.1"
                     /translation="NRFTLPPLTIAVIARLGEDEKIPVSERQATASCVCLLLLQAAQA
                     EGFGAQWLTGWPAYDRPLLERTLGLAPDEQLAGTIAMGTPQVDVPDRERPDPASLLTD
                     WSPA"
     gene            320..1228
                     /locus_tag="PK399_11095"
     CDS             320..1228
                     /locus_tag="PK399_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HPW13706.1"
                     /translation="MSQPVSASNTLYLVDASMYVFRAWHSMPDEFHDADGWPTNAVHG
                     FARFLLELLERERPRHIAIAFDEALDSCFRNALYPAYKANRDPAPPELRRQFAWCKGL
                     CQALGLQTLAHHDYEADDLIGSAVHRARGHGYRSVIVSADKDLSQLLDVHDEQYDFAK
                     GQRWGRDGVKARHGVQAHQIADYLALTGDAVDNIPGITGIGPKSAAVLLAHFDSLDAL
                     LARIDEVPFLRLRGAAQLAVRLREQREHALLWRQLTTIALDAPLPDGHFARGNGDADE
                     LQALSDWIGFGPLTRKRLAEAAGLSA"
     gene            1396..1941
                     /locus_tag="PK399_11100"
     CDS             1396..1941
                     /locus_tag="PK399_11100"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPW13707.1"
                     /translation="MGHNLKTTLYEGDWLRLVKIGHWESCERTHGQGMAVIVIAVTPA
                     DEVLFVEQYRIPLGSRTIEMPAGLVGDDHAQDTLVDAARRELIEETGWSPGKVDVLLV
                     GPTSSGMSNERIAFVRALDLVRVGDGGGVDSEDITVHHVPRAEAPAWLMRKHAEGFEL
                     DLKLWAGLWMIEHDPDGSPRR"
     gene            2021..3079
                     /gene="ppk2"
                     /locus_tag="PK399_11105"
     CDS             2021..3079
                     /gene="ppk2"
                     /locus_tag="PK399_11105"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HPW13708.1"
                     /translation="MARRPTAKPPVAKPRATPAARKPRSRLPAPDPIPTPAAAAVAQA
                     EAEAWAGTHAPPAVPPMGIAPAELGVPEATAREMEAMPVTAEESTSAPLPAGYPYRVR
                     LRRREYEAEKKKLQIELLKVQSWVKESGQRIVMLFEGRDAAGKGGTIKRFTEHLNPRG
                     ARVVALEKPSERERGQWYFQRYIEHLPTYGEIVLFDRSWYNRAGVERVMGFCTPAEYL
                     EFLREAPEFERMLVRSGVRLYKFWFSVSREEQLRRFNSRRNDPLKHWKLSPIDVQSLG
                     KWDDYTDAKEAMFFHTDTADSPWLVIKSDDKKRARLNCMRHVLSTLPYPGKDPKVAYP
                     ADPLIVGSAANVLAADGQ"
     gene            complement(3092..4036)
                     /gene="pip"
                     /locus_tag="PK399_11110"
     CDS             complement(3092..4036)
                     /gene="pip"
                     /locus_tag="PK399_11110"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HPW13709.1"
                     /translation="MRTLYPELAPSRTGTLAVDGRHTLYWEECGNPDGKPVVLLHGGP
                     GAGCNANMRRFHDPAKYRIVLFDQRGSGRSTPHADLVDNTTWDLVADIEKLREHLGIA
                     RWQVFGGSWGSTLALAYAETHPQRVTELVLRGIFMLRRWELEWFYQEGASRLFPDAWA
                     HYIAAIPEVERADLISAFHRRLTSDDAGVRLAAARAWAIWEGGTSYLRIPADYADTHG
                     NAEFALAFARIENHYFVNGGFFEVEDQLLRDAHRIADIPGVIVHGRYDVVCPIQNAFD
                     LHKAWPKAELLVSATAGHSAFEAENAAALVEGTDRFAG"
     gene            complement(4082..4915)
                     /gene="prmC"
                     /locus_tag="PK399_11115"
     CDS             complement(4082..4915)
                     /gene="prmC"
                     /locus_tag="PK399_11115"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPW13710.1"
                     /translation="MQSTTPRVRDLLQRASAMIERVDAEWLLAHALDRPPGWLWAHAD
                     DAVEAAARERFEALLSRRAAGEPVAYLSGTQGFWSLDLRVTPATLIPRPETERLVELA
                     LARLPADAPCRVADLGTGSGAIALAIARERPMAAVVATDASKAALAVAVANAQRNGID
                     NAWFRRGDWCEALGRERFDLIASNPPYIALGDPHLGQGDLRHEPPDALASGSDGLDAI
                     RRIVRDAPAHLAPAGWLLLEHGWDQGEAVRALMAAAGFVDVATEPDLEGRDRVTLGRR
                     P"
     gene            5096..>5384
                     /locus_tag="PK399_11120"
     CDS             5096..>5384
                     /locus_tag="PK399_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017114658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPW13711.1"
                     /translation="MSLINTKVAPFKANAFHNGKFVEVTDQSLQGKWSVLIFMPAAFT
                     FNCPTEIEDAAEHYAEFQKAGAEVYIVTTDTHFSHKVWHETSPAVGKAKFPL"
BASE COUNT          843 a         1905 c         1869 g          767 t
ORIGIN      
        1 gcaaccgctt caccctgccg ccgctgacca tcgcggtcat cgccaggctg ggcgaggacg
       61 agaagatccc ggtctccgaa cgccaggcca cggcctcctg cgtctgcctg ttgttgctgc
      121 aggccgcgca ggccgagggc ttcggcgcgc aatggctgac cggctggcca gcctacgacc
      181 gcccgttgct ggaacgcacg ctggggctcg cacctgatga gcaactggcc ggcaccattg
      241 cgatggggac gccgcaggtc gacgtaccgg accgcgaacg gccggatccg gcgtcgctgc
      301 tgacggactg gtcgccggcg tgagtcagcc agtgtcggcc tcgaacacgc tctacctcgt
      361 cgatgcgtcg atgtacgtgt tccgcgcctg gcattccatg ccggacgagt tccacgacgc
      421 cgacggctgg ccgaccaacg cggtgcatgg cttcgcccgc ttcctgctgg agctgctgga
      481 gcgcgaacgc ccgcggcaca tcgccatcgc cttcgacgag gcgctggact cctgcttccg
      541 caacgcgcta tatccggcct acaaggccaa ccgcgacccc gcgccgccgg agctgcgccg
      601 ccagttcgcc tggtgcaagg gcctgtgcca ggcgctgggg ctgcagaccc tggcccacca
      661 cgactacgag gccgacgacc tgatcggcag cgccgtgcac cgcgcgcgcg gacacggcta
      721 ccgcagcgtg atcgtgtccg ccgacaagga cctctcgcag ctgctggacg tgcacgacga
      781 gcagtacgac ttcgccaagg gccagcgctg gggccgcgac ggggtcaagg cgcggcacgg
      841 cgtgcaggcg caccagatcg ccgactacct ggcgctcacc ggcgacgcgg tcgacaacat
      901 cccgggcatc accgggatcg gcccgaagtc ggccgcggtc ctgctcgcgc atttcgattc
      961 gctggatgcc ctgctggcgc ggatcgacga ggtgccgttc ctgcgcctgc gcggggccgc
     1021 gcagctcgcc gtgcgcctgc gcgaacagcg cgagcacgca ctgctgtggc ggcaactcac
     1081 cacgatcgcg ctggatgcgc cgttgccgga cggccatttc gcgcgcggca acggcgacgc
     1141 cgacgaactg caggcgctgt cggactggat cggcttcggt ccgctgaccc gcaagcgctt
     1201 ggccgaggcg gcggggcttt ctgcctgaag cgcccccgag catggaagcg ctcgtccggg
     1261 ggagggttcg agcgcgccat ggatggcgcg cggccgcgca tcggagccgg atgcggagcc
     1321 taagcggcga atcccctccg ggcgagcgct tcattgcgaa cgatgatggt cacccgcctg
     1381 cggtagcatc gaggcatggg ccacaacctc aagaccaccc tgtacgaagg cgactggctg
     1441 cggctggtga agatcggcca ctgggaatcc tgcgagcgca cccacggcca gggcatggcg
     1501 gtgatcgtga tcgcggtgac cccggcggac gaggtcctgt tcgtcgagca gtaccgtatc
     1561 ccgctgggtt cgcgcaccat cgaaatgccc gcgggcctgg tcggcgacga ccatgcgcaa
     1621 gacaccctgg tcgatgccgc gcgtcgcgaa ctgatcgagg aaaccggctg gtcgccgggc
     1681 aaggtcgacg tgctgctggt cggcccgacc agttccggca tgagcaacga gcgaatcgcc
     1741 ttcgtgcgcg cgctggacct ggtccgggtc ggcgacggcg gcggcgtgga cagcgaggac
     1801 atcaccgtcc accacgtgcc gcgggccgag gcgccggcct ggctgatgcg caagcatgcc
     1861 gagggcttcg agctcgacct caagctctgg gccgggctgt ggatgatcga gcacgacccg
     1921 gacggcagcc cgcgccgctg acccgggatt gacggcggtc aaggccgcgg cccgctgctg
     1981 gtgcgatgat gggatctcgc catcgaccca ggaacgcgcc atggcccgtc gcccgaccgc
     2041 caagcccccc gtcgccaagc cgcgggccac ccccgccgcg cgcaagccgc gcagccggct
     2101 gcctgcgccc gacccgatcc ccaccccggc cgccgccgcg gtcgcgcagg ccgaggccga
     2161 agcctgggcc ggcacccacg cgccgccagc ggtgccgccg atgggcatcg cgcccgcaga
     2221 actcggcgtg ccggaggcca ccgcgcggga gatggaggcg atgccggtca ccgccgaaga
     2281 gagcacctcc gcgccgctgc ccgccggcta tccctaccgc gtccgcctgc gccgcaggga
     2341 atacgaggcg gagaagaaga agctgcagat cgagctgctg aaggtgcaga gctgggtcaa
     2401 ggagagcggg cagcgcatcg tgatgctgtt cgagggccgc gacgccgccg gcaagggcgg
     2461 caccatcaag cgtttcaccg agcacctcaa cccgcgcggg gcgcgcgtgg tcgcgctgga
     2521 gaagcccagc gagcgcgaac gcgggcagtg gtacttccag cgttacatcg agcacctgcc
     2581 gacctacggc gagatcgtgc tgttcgaccg cagctggtac aaccgcgccg gggtcgagcg
     2641 ggtgatgggc ttctgcaccc cggcggaata cctcgagttc ctgcgcgagg cgccggagtt
     2701 cgagcgcatg ctcgtgcgca gcggcgtccg cctctacaag ttctggttct cggtcagccg
     2761 cgaggaacag ctgcggcgct tcaactcgcg ccgcaacgac ccgctgaagc actggaagct
     2821 gtcgccgatc gacgtgcagt cgctgggcaa gtgggacgac tacaccgacg ccaaggaagc
     2881 gatgttcttc cacaccgaca ccgccgactc gccgtggctg gtgatcaagt ccgacgacaa
     2941 gaagcgcgcg cgcctcaact gcatgcgcca cgtgctgtcg accctgccct acccgggcaa
     3001 ggacccgaag gtcgcctacc cggcggatcc gctgatcgtc ggctcggcgg ccaatgtgct
     3061 cgccgcagac ggccagtagg cggcgcgcgc ttcagccggc gaagcggtcg gtcccctcca
     3121 ccagcgcggc ggcgttctcg gcctcgaacg cggagtggcc ggcggtcgcg ctgaccagca
     3181 gctccgcctt cggccaggcc ttgtgcaggt cgaaggcgtt ctggatcggg cagaccacgt
     3241 cgtaacggcc gtgcacgatc accccgggga tgtcggcgat gcgatgggca tcgcgcagca
     3301 gctggtcctc gacctcgaag aagccgccgt tgacgaagta atggttctcg atccgcgcga
     3361 acgccagcgc gaactccgcg ttgccgtggg tgtcggcgta gtccgcgggg atccgcaggt
     3421 agctggtgcc gccttcccag atcgcccagg cgcgcgcggc cgccagccga acgccggcat
     3481 cgtcggaggt caggcggcga tggaaggccg agatcaggtc cgcgcgctcc acctccggga
     3541 tcgccgcgat gtagtgcgcc cacgcatccg ggaacaggcg gctggcgcct tcctggtaga
     3601 accattccag ctcccagcgg cggagcatga agatgccgcg caggaccagt tcggtcaccc
     3661 gctgcgggtg ggtctcggca taagccagcg cgagggtcga gccccagctg ccgccgaaca
     3721 cctgccagcg cgcgatgccg aggtgctcgc gcagcttctc gatgtcggcg accaggtccc
     3781 aggtggtgtt gtcgacgagg tcggcgtgcg gggtgctgcg ccccgagccg cgctggtcga
     3841 acagcacgat gcggtacttc gccgggtcgt ggaagcgccg catgttggca ttgcagcccg
     3901 cgccgggacc gccgtgcagc aacaccaccg gcttgccgtc cgggttgccg cattcctccc
     3961 agtacagggt gtggcggccg tcgaccgcga gggtgccggt ccgggacggg gcgagttcgg
     4021 gatacagcgt gcgcatggcg ggatccggcg gtggagaacc ccggattcta gctggcgcgg
     4081 ttcagggcct gcgccccagg gtcacgcggt cgcggccctc gaggtcgggc tcggtcgcca
     4141 cgtccacgaa gccggccgcg gccatcagcg cacgcacggc ctcgccctgg tcccagccgt
     4201 gctccagcag caaccagccc gcgggcgcca ggtgcgccgg ggcgtcgcgg acgatgcggc
     4261 ggatcgcgtc cagtccgtcg ctgcccgagg cgagcgcgtc gggcggttcg tggcggaggt
     4321 cgccctggcc gaggtgcgga tcgccgagcg cgatgtacgg cggattgctg gcgatcaggt
     4381 cgaagcgctc gcgaccgagc gcctcgcacc agtcgccgcg acggaaccac gcattgtcga
     4441 tgccgttgcg ttgcgcgttg gccaccgcca ccgccaaggc cgccttgctg gcgtcggtgg
     4501 cgaccaccgc ggccatcggc cgctctcggg cgatggccag cgcgatcgcc ccgctgccgg
     4561 tgccgaggtc cgcgacccgg cagggggcgt cggcgggcag ccgcgccagg gcgagttcga
     4621 ccagccgttc ggtttccggg cgcgggatca gggtggccgg cgtcacccgg aggtccagcg
     4681 accagaagcc ttgggtcccg ctcaggtagg cgaccggctc gccggcggcg cgacgcgaca
     4741 gcaaggcctc gaaacgctcc cgggcagccg cctcgaccgc gtcgtcggca tgcgcccaca
     4801 gccagcccgg cgggcggtcc agcgcatgcg ccagcagcca ctcggcatcg acgcgctcga
     4861 tcatggcgct ggcgcgctgc aacaaatccc ggacccgcgg cgtggtgctc tgcatgcgtg
     4921 cagtgtcgca tcgcgtggct ggaagccgca ttgacgcgtc gcgtcggcga cgtcgaccga
     4981 ttcaatagtt cttaactatt gattcgatag aatcaatttc attgattgat tgaatgccac
     5041 gccatagatt tgtcgcgtcg catcatccca cccccgccca caccgaggaa ccacgatgtc
     5101 cctgatcaac accaaggtcg ccccgttcaa ggccaatgcc ttccacaacg gcaagttcgt
     5161 cgaggtcacc gaccagtccc tgcagggcaa gtggtcggtc ctgatcttca tgccggcggc
     5221 cttcaccttc aattgcccga ccgagatcga agacgccgcc gagcactacg ccgagttcca
     5281 gaaggccggc gccgaggtct acatcgtcac caccgacacc cacttctctc acaaggtgtg
     5341 gcacgaaacc tcccccgcgg tgggcaaggc caagttcccg ctgg
//
LOCUS       DAOOCX010000154         5377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_14339_length_5377_cov_9.180571, whole genome shotgun sequence.
ACCESSION   DAOOCX010000154 DAOOCX010000000
VERSION     DAOOCX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14339_length_5377_cov_9.180571"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1243
                     /locus_tag="PK399_11125"
     CDS             137..1243
                     /locus_tag="PK399_11125"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPW13712.1"
                     /translation="MTTPTAAPLAYRVTPSTHARTDEARAALLAGDLGFGKLMTDHMV
                     TIEWDSARGWHDAQVHAYGPLSLDPAAAVLHYGQEIFEGIKAYRHADGSIWTFRPRAN
                     GERLQRSAARLALPQLPVDEFVESLRQLVAIDHAWVPSAPETSLYFRPFMIATEAFLG
                     VRAAQAAAYHVIASPAGAYFANGVSPVSIWLSQDYARAAKGGTGAAKCGGNYAASLLP
                     QLEARAHGCSQVLFLDPVEGKYLEELGGMNVFLVYADGRLVTPALSGSILEGITRSSI
                     LQLARDRGLQVEERQVSIDEWRDGVASGAITEAFACGTAAVITPIGELKGKDLSIGDA
                     NAPAGQVTMSIRKELTDIQYGRLPDRHGWLVRLR"
     gene            complement(1262..1840)
                     /locus_tag="PK399_11130"
     CDS             complement(1262..1840)
                     /locus_tag="PK399_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13713.1"
                     /translation="MHRAALLFLLSIVGLLAPGAVMAQVKRCTAADGTLVYTDRKCDD
                     IGATERVGAMPSAGGAIAGRYRNVCARTVQDLVYSFSNALQSGDANQVAGVYDWAGMP
                     TSTAYNVMTRLEAIAKRPLIDVQPMYSGDTNAYGDDIVTFDETTGEVVTRAPRKPRLI
                     GLRVEQTLANGTTPSRTNFGLRQRLGCWWVRL"
     gene            complement(1886..2521)
                     /locus_tag="PK399_11135"
     CDS             complement(1886..2521)
                     /locus_tag="PK399_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13714.1"
                     /translation="MRILPTSLLAAALVLGALPMVAPRVEAKTGVLRCQMADGSYAYT
                     NKACSAFGAKSTPLPGEVLNRIERDQRREARLNGIELADASSMPLQAGVRRSVQRGCA
                     GDPRQLAADLTASVAIRDVNRVAESFDWAGMSNAQAQRVMSRLEHLSTQLVTDAEYFD
                     ASMGGQVMFADSRGGSDGAAGQMQVTVVDDGNESVLDFDVRRDEGCYFLQY"
     gene            complement(2615..3439)
                     /gene="metF"
                     /locus_tag="PK399_11140"
     CDS             complement(2615..3439)
                     /gene="metF"
                     /locus_tag="PK399_11140"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HPW13715.1"
                     /translation="MLPISFEFYPPKTDEQRTQLDRSVQKLKAHAPQFMSVTFGAGGS
                     TLSHTPETVQRLRSEHGVAGVPHLTCVGGNREEIRSLLKLYRALGCDRIVALRGDTPS
                     GMVRSGDLRYANELVELIRTEHGNHFTIEVAAYPETHPQAEDAMADLRNFKRKVDAGA
                     DSAITQYFYNTDAYFRFVDDARALGIDIPIIPGIMPISNFSQLRRFSEACGAEIPRWI
                     AQRMRGYGDDAESIREFASDFVAQLCERLIAGGAPGLHFYTLNLAKPSLNVLAKLA"
     gene            3573..4718
                     /locus_tag="PK399_11145"
     CDS             3573..4718
                     /locus_tag="PK399_11145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW13716.1"
                     /translation="MQHVPDHVVATAQAPPRLLQELRETLQRLRLGGPFYVLLWLLAG
                     VAADLWLRAPVAFSLITFGFVALTVVRFRVHGLAEGTQEDDVRGRLDFIWLLLLVNAG
                     LWGAASAWLLAIAPEESARTVAAISSYAFATAFAHNFPMRLRHAFGAVVLIYLPTLAA
                     LIASGARYELIGVSALYLLYVSLALRRSHAEYLQRLDLEDELRQQRDLFEQQSRRDGL
                     TGLANRRRFSSALEDWSAQARSNRRPLSLLILDLDHFKAINDQHGHARGDACLRAFAS
                     RLQEAFAGNGELVARLGGEEFGVLLRETSLNEAFTHAEAFRAGLAADPLDTGDGIGLA
                     LRVSIGVAAFEPGVHHDADALYHAADTALYEAKASGRNTVRQVAAID"
     gene            complement(4806..>5377)
                     /locus_tag="PK399_11150"
     CDS             complement(4806..>5377)
                     /locus_tag="PK399_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPW13717.1"
                     /translation="DPINALQASMEGFEVNTVESTLGRGDIYVTTTGNKDVLTIEHLS
                     QMKDQAIVCNIGHFDNEIQVDALNAFPGVTKLNIKPQVDKYTFPDGRAIFLLAEGRLV
                     NLGCATGHPSFVMSNSFSNQTLAQIDLWANKDSYKPAVYILPKKLDEEVARLHLEKIG
                     VKLTTLTAEQAAYLGVDVDGPYKPEHYRY"
BASE COUNT          852 a         1719 c         1859 g          947 t
ORIGIN      
        1 cgcctgtgcg cgctgtatgg ctgggatgtg cgggtgcgtc cgggcgagga caccggcgtg
       61 gtggcaacgc tgcgcttcgg ataaggcgcg ccggcgcgtc ataatgcacg ggctcgattt
      121 tctccaggac gcccgcatga ccacccccac cgcagccccg ttggcctacc gggtcacgcc
      181 ctccacgcat gcacgaaccg acgaagcacg cgctgcgttg ctcgccggcg acctcgggtt
      241 cggcaagctc atgaccgacc acatggtcac catcgaatgg gacagcgcac gcggctggca
      301 cgatgcgcag gtgcatgcct acgggccact gtcgctggat cctgccgccg cggtgctgca
      361 ctacggccag gagatcttcg aaggcatcaa ggcgtatcgc cacgccgacg gctccatctg
      421 gaccttccgg ccccgcgcca acggcgagcg cctgcagcgc tccgcggcgc ggctggcgtt
      481 gccgcaattg ccggtcgacg agttcgtgga gtcgctgcgc cagctggtgg cgatcgacca
      541 cgcatgggtg ccgtcggcac cggaaacgag cctgtacttt cgtcccttca tgatcgccac
      601 cgaggccttc ctcggcgtgc gcgcggcgca ggccgcggcc taccacgtca tcgccagccc
      661 ggccggcgcg tacttcgcca acggcgtttc gccggtgtcg atctggctgt cgcaggacta
      721 cgcgcgcgcg gccaagggcg gcaccggcgc ggccaagtgc ggcgggaact acgccgcgtc
      781 gctgctgccg cagctggaag cgcgcgcgca cgggtgttcg caggtgctgt tcctcgatcc
      841 ggtcgagggg aagtacctgg aagaactcgg tggcatgaat gtgttcctgg tgtacgcgga
      901 tggccgcctg gtcaccccgg ccctgtccgg cagcatcctc gagggcatca cccgttccag
      961 catcctgcaa ctggcgcgcg accgcggcct gcaggtcgaa gaacgccagg tgtcgatcga
     1021 cgagtggcgc gatggcgtgg cgtcgggtgc catcaccgag gccttcgcct gtggcaccgc
     1081 agcggtgatc accccgatcg gcgagttgaa gggcaaggac ctcagcatcg gcgatgccaa
     1141 cgcaccggca ggccaggtga cgatgtcgat ccgcaaggaa ctgaccgaca tccagtacgg
     1201 ccggctgccc gaccgccacg gttggctggt caggctgcgc tgagcggcgc gtcgaccgcg
     1261 atcagagccg cacccaccag cagcccaggc gctggcgcag gccgaagttg gtgcgtgagg
     1321 gcgtggtgcc gttggccagc gtctgttcga cgcgcaagcc gatcaggcgc ggcttgcgcg
     1381 gcgcgcgggt gaccacttcc ccggtggtct cgtcgaacgt gacgatgtcg tcgccgtagg
     1441 cgttggtgtc gccgctgtac atcggttgca cgtcgatcaa cgggcgcttg gcgatcgcct
     1501 ccaggcgcgt catcacgttg taggccgtgg aggtcggcat gccggcccag tcgtagacgc
     1561 cggccacctg gttggcatcg ccagattgca aggcgttgct gaaggaatag accaagtcct
     1621 gcacggtgcg tgcgcagacg ttgcggtaac ggccggcgat cgcgcctccc gccgatggca
     1681 ttgcgccgac gcgttcggtc gcgccgatgt cgtcgcactt gcggtcggtg tagaccagcg
     1741 tgccatccgc ggcggtgcaa cgcttcacct gcgccatcac cgcgcccggc gcaagcaggc
     1801 cgacgatcga caaaagaaag agcagggcgg cgcgatgcat gccgcgcagc ctaaccgcgc
     1861 gcgctgcatc gaggaagtgc gccggtcagt actgcaggaa gtagcagcct tcgtccctgc
     1921 gaacgtcgaa gtcgagaacc gattcgtttc cgtcatcaac gaccgtgacc tgcatctggc
     1981 cagcggcgcc atcggatccg ccacgggagt ccgcgaacat cacctgcccg cccatgctgg
     2041 cgtcgaaata ctccgcatcc gtcaccaact gcgtggacag gtgttcaagg cgcgacatca
     2101 cgcgttgcgc ctgcgcattc gacatgcccg cccaatcaaa gctttccgcg acgcggttga
     2161 cgtcacgaat cgccacggat gcggtcaggt ccgccgccag ttgcctgggg tcgccggcgc
     2221 aaccacgctg caccgaccgg cgaacgcctg cctgcaatgg catagaggac gcgtcggcaa
     2281 gctcgatgcc gttcaagcgc gcctcacgcc gttggtcgcg ttcgatccgg ttcaagacct
     2341 cgccgggcag gggtgtggac ttcgctccga acgcgctgca agccttgttg gtgtaggcat
     2401 agctgccgtc cgccatttgg caacgcagca cccctgtctt cgcttcgacg cggggagcga
     2461 ccatcggaag ggcaccgagc acgagggcgg ctgcgagcaa cgaggtaggc aggatgcgca
     2521 tggcgtgggg caggtgtcat gccgggattg gcaggcgcag gatcccgtcg acccggtcaa
     2581 cgccgcgtgc gaaaacgccg aacctcccct gaattcacgc caatttagcg agcacgttca
     2641 gagagggttt cgccaggttc aacgtgtaga agtgcagccc cggcgcaccg ccggcgatca
     2701 ggcgctcgca tagctgcgca acgaaatcgg aggcgaattc gcggatgctc tcggcgtcgt
     2761 cgccatagcc acgcatgcgc tgcgcgatcc agcgcgggat ctcggcgccg caggcttccg
     2821 agaagcgccg cagttggctg aagttggaga tcggcatgat cccggggatg atcgggatgt
     2881 cgatgccgag tgcgcgcgca tcgtcgacga aacggaagta ggcgtcggtg ttgtagaagt
     2941 actgggtaat cgcgctgtcg gcgccggcat cgaccttgcg cttgaagttg cgcaggtcgg
     3001 ccatcgcgtc ttccgcctgc ggatgggttt ccggataggc cgcgacttcg atggtgaagt
     3061 ggttgccgtg ctcggtgcgg atcagttcga ccagctcgtt ggcgtagcgc aggtcgccgc
     3121 tgcggaccat gcccgagggc gtgtcgccgc gcagcgcgac gatgcggtcg cagcccagcg
     3181 cgcgatagag cttgagcagc gagcggatct cctcgcggtt gccgccgacg caggtcaggt
     3241 gcggcacgcc ggccacgccg tgctcgctgc gcaggcgctg cacggtttcc ggggtgtggc
     3301 tgagggtcga gccaccggcg ccgaacgtca cagacatgaa ttgcggtgcg tgcgccttga
     3361 gcttctgcac gctgcggtcc agctgcgtgc gctgttcgtc ggtcttgggc ggatagaact
     3421 cgaagctgat cggcagcatg gcgtcgttcg cgtgcaggat gcgatgattg tatctcttca
     3481 tccggatgaa gcaaaagata aagtcgttcg gctggaaggg catgcttgac gcccttggag
     3541 ccggccatgc atcgtgcagg cagggggaat ccatgcaaca cgttccagac cacgtcgtcg
     3601 ccacggcgca ggcgccgccg cgcctgctgc aggaattgcg cgaaacactg caacggttgc
     3661 gcctgggcgg tccgttctac gtgctgctgt ggctgctggc cggcgtggcc gcagacctgt
     3721 ggctgcgcgc acccgtggct ttcagcttga tcaccttcgg tttcgttgcc ctgaccgtgg
     3781 tgcggttccg tgtccacgga ttggccgagg gcacccagga agacgatgtc cgcgggaggc
     3841 tcgacttcat ctggctgctg ttgctggtga atgccgggtt gtggggcgcg gcgtcggcat
     3901 ggctgctggc gattgcgccg gaggaaagcg cgcgcacggt cgccgcgatc agttcctatg
     3961 ccttcgccac cgcgttcgcg cacaacttcc cgatgcgcct gcggcacgcc ttcggtgcgg
     4021 tggtgctgat ctacctgccg accctggcgg cattgatcgc cagcggtgcg cgttacgaac
     4081 tgatcggcgt cagcgcgctg tacctgcttt acgtgagcct ggcattgcgc cgcagccacg
     4141 ccgagtacct gcagcggctg gatctggagg atgagttgcg ccaacagcgc gacttgttcg
     4201 agcagcaaag ccggcgcgat ggattgaccg ggctggccaa ccggcgccgg ttctcgtcgg
     4261 cgctggagga ctggtcggcg caggcgcgga gcaaccgcag gccgctgtcg ctgctgatcc
     4321 tcgacctcga ccatttcaag gcgatcaacg atcagcacgg acatgcgcgt ggcgatgcct
     4381 gcctgcgcgc gttcgccagc cgcctgcagg aagcgttcgc ggggaacggg gagttggtgg
     4441 cgcggctggg cggcgaagaa ttcggtgtct tgctgcgcga gacctcgttg aacgaggcat
     4501 tcacgcatgc agaagcgttc cgtgccgggc tggcagcgga tccactcgat accggcgacg
     4561 gcatcggcct ggcgctgcgg gtgagcatcg gcgtggccgc gttcgagccc ggcgtgcacc
     4621 acgatgccga tgccctgtac cacgcagccg ataccgcgct gtacgaggcc aaggcctcgg
     4681 gccgcaatac ggtgcggcag gtcgcggcga tcgactgagc gcgaaggccc gcgatccgcg
     4741 gatccatgaa cgaaaagggc ggccagtggc cgccctctcc ttgatcgccg cggcagtgcc
     4801 gcgactcagt agcggtagtg ctccggcttg taggggccgt ccacgtccac gcccaggtag
     4861 gcggcctgct cggcggtgag ggtggtcagc ttcacgccga tcttctccag gtgcaggcgc
     4921 gccacttctt cgtccagctt cttcggcagg atgtagacgg ccggcttgta gctgtccttg
     4981 ttggcccaca ggtcgatctg cgcgagcgtc tggttggaga acgagttgct catcacgaag
     5041 ctcgggtggc cggtggcgca gcccaggttg accagtcggc cttcggccag caggaagatc
     5101 gcgcggccat ccgggaacgt gtacttgtcc acctgcggct tgatgttgag cttggtgacg
     5161 cccgggaagg cgttgagcgc atcgacctgg atctcgttat cgaagtggcc gatgttgcag
     5221 acgatcgcct ggtccttcat ctggctcagg tgctcgatgg tcagcacgtc cttgttgccg
     5281 gtggtggtga cgtagatgtc gccacggccc agggtcgatt ccacggtgtt gacctcgaag
     5341 ccttccatcg acgcctgcag ggcgttgatc gggtcga
//
LOCUS       DAOOCX010000155         5330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_14493_length_5330_cov_7.450047, whole genome shotgun sequence.
ACCESSION   DAOOCX010000155 DAOOCX010000000
VERSION     DAOOCX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14493_length_5330_cov_7.450047"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PK399_11155"
     CDS             complement(<1..460)
                     /locus_tag="PK399_11155"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808006.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPW13718.1"
                     /translation="MPMSSRHDPSRSPRAPRGNTLTCKSWLTEAPFRMLQNNLDAEVA
                     EDPTSLVVYGGIGRAARDWDCYDAIIESLKNLNDDETLLVQSGKPVGVFPSHADAPRV
                     LIANSNLVPHWATWEHFHDLDKKGLMMYGQMTAGSWIYIGSQGIVQGTYET"
     gene            558..1778
                     /locus_tag="PK399_11160"
     CDS             558..1778
                     /locus_tag="PK399_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HPW13719.1"
                     /translation="MAFLLRGALLALLLLVQGCATTRAPDAAPTVVLVSIDGLPAAVP
                     GSGRMPALDGIAREGVQAAWLQPSYPTLTFPNHYTLATGLRPDRHGIVNNNINDPVLG
                     RYVSKQASARDGRWYGGEPIWATLQRQGGIAATMFWPGSEAEIAGQRPRYWTPFDGSL
                     PVDARVDQLLAWLDLPPAQRPRLLTLYMEQYDVASHASGMHSLQAVQALSTIDAGLAR
                     LRDGLHARGLDDRVDLIVLSDHGMADVPAANLVFLDDLLPADAMQVESVGQMAWLTAK
                     PGREAEVERALVRRHDHFECWRKADAPPAWHFGRNPRIPPIICQADDGWRVWLHRYPV
                     STVLKGEHGFAPEDVGMRAAFTAVGPSFRNGVRLPAFDNVDVYALLARLLRITPAAND
                     GDIAPLLPALKDSR"
     gene            1775..2272
                     /locus_tag="PK399_11165"
     CDS             1775..2272
                     /locus_tag="PK399_11165"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPW13720.1"
                     /translation="MILCRRIDSPVGPLMLAADDSGLRHIEFRDNRHPVNRGDWHGGD
                     NAILQATEAQLAEYFAGTRTRFDLPLAPRGTEFQLRVWQALARIPHGSTSTYAEQARA
                     IGSPDAVRAVGAANGRNPLPIVLPCHRVIGADGALVGFGGGLPIKEFLLRLEGALPAR
                     QAGLF"
     gene            complement(2313..>3053)
                     /locus_tag="PK399_11170"
     CDS             complement(2313..>3053)
                     /locus_tag="PK399_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW13721.1"
                     /translation="RIGCALHSLRAVPSDPMHEVLTALPAASRIHIHIAEQLPEVEEC
                     IALRGARPVQWLLDSVPVDERWTLVHATHLDDDEVQDIAGSGATVAICTSTEANLGDG
                     LFPLRDYLDAGGRWGVGSDSNSSVDPVEELRWLEYGQRLRVQRRNIASSMRMPSVGAT
                     LLDGAHASAADSTGFAAGDRIVLDADAPILAGATDGDVIDRWLFSGNRPLVRDVEVAG
                     VRVVEDGRHRDRDAIATRYRDAMKQLLR"
     assembly_gap    3054..3153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3154..3709)
                     /locus_tag="PK399_11175"
     CDS             complement(<3154..3709)
                     /locus_tag="PK399_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPW13722.1"
                     /translation="MHTPTTAIATRTPRGWALPGIANLHSHAFQRAMAGLAERQTDPA
                     DSFWTWRETMYAMAARFDPDLLHAVASQLYAEMLEAGYTTVCEFHYLHHAPDGTPYAQ
                     ADAMSQALLRAARDTGIRITLLPVLYMTGGFDGRPLSARQQRFGHSVDAYLRLLETLR
                     ASESDTVRIGCALHSLRAVPSDPMH"
     gene            3758..3949
                     /locus_tag="PK399_11180"
     CDS             3758..3949
                     /locus_tag="PK399_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13723.1"
                     /translation="MDGMLVIGMMFAAMALAVVLILHLRRRYGSGNGGDGGYGGESGH
                     SHHDHDGGCDGGGDGGGGD"
     gene            3939..5159
                     /gene="hutI"
                     /locus_tag="PK399_11185"
     CDS             3939..5159
                     /gene="hutI"
                     /locus_tag="PK399_11185"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPW13724.1"
                     /translation="MVTERWDGLLLGPTLATLDTGTGYGLVEDGALGWNDGLLTYVGP
                     RSGLPGDAPSLADDVIEAEGLVTPGLVDCHTHLVFAGDRASEFELRLQGASYEDIARS
                     GGGILSSVRAVREASEDELLAQSLPRARALIADGATTLEIKSGYGLDLANEGKMLRVA
                     RRIGETLGICVRTTFLGAHALPPEYRDDADGYIDAAIGWLHALHAEGLVDAVDAFCER
                     IGFSSAQTRRMFEAARGLGLPVKLHADQLSDGGGAALAAEFNGLSADHIEYTDEGGVR
                     AMADAGTVAVLLPGAFHVLRETKLPPLDLLREHSVPMAVATDCNPGTSPLLSLRQAMQ
                     LACTHFKLTPEEALRGTTINAARALGLHDRGTLRVGLRADFAHWRVARPAALCYWLGG
                     QLLDASYAGGRRLV"
     gene            5134..>5330
                     /locus_tag="PK399_11190"
     CDS             5134..>5330
                     /locus_tag="PK399_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13725.1"
                     /translation="MPAVAGSSESLETFMRHAALAASILVSLALAAPIAQAQDAATRL
                     AQDAIIVDTHIDAPGILMDKWA"
BASE COUNT          792 a         1772 c         1820 g          846 t
ORIGIN      
        1 aggtctcgta ggtgccctgc acgatgccct ggctgccgat gtagatccag ctgcccgcgg
       61 tcatctgccc gtacatcatc aggccctttt tatcgagatc gtggaagtgt tcccacgtgg
      121 cccagtgcgg caccaggttg gagttggcga tgagcacgcg cggcgcgtcg gcatggctgg
      181 ggaacacgcc gaccggcttg ccggattgca ccagcagggt ttcgtcgtcg ttgaggttct
      241 tgagcgattc gatgatggcg tcgtagcagt cccagtcgcg cgcggcgcgg ccgatgccgc
      301 cgtagaccac cagcgaggtg gggtcttcgg ccacttccgc gtccaggttg ttctgcaaca
      361 tgcggaacgg cgcctcggtc agccacgact tgcaggtgag cgtgttgccg cgcggcgcgc
      421 ggggggagcg ggacggatcg tggcggctgg acatcggcat ggctcctgca agcgaagccg
      481 cgattatcgc accggcaccc ggcccgactc agcggtatgg aagccgaggt gtcatcggcc
      541 tccgctatca tcggcgcatg gctttcctgc tccgcggcgc actgctggcg ctgctcctcc
      601 tcgtccaggg ctgcgccacc acgcgcgcgc ccgacgccgc gccgacggtg gtgctggtct
      661 cgatcgacgg cctgcctgcc gcggtccccg gcagcgggcg catgcccgcg ctggacggca
      721 tcgcccgcga aggcgtgcag gcggcgtggc tgcagccgag ctaccccacc ctgaccttcc
      781 ccaaccacta cacgctggcg acggggctgc gtccggaccg ccacggcatc gtcaacaaca
      841 acatcaacga ccctgtgctg gggcgctacg tgtccaagca ggccagcgca cgcgacggcc
      901 gctggtacgg cggcgagccg atctgggcca cgctgcagcg gcagggcggg atcgcggcga
      961 cgatgttctg gccaggctcg gaagccgaga tcgccgggca gcgtccgcgc tactggacgc
     1021 cttttgacgg cagcctgccg gtcgatgcac gcgtcgatca attgctggcc tggctggacc
     1081 tgccgcccgc gcagcgcccg cgcctgctca ccctgtacat ggagcagtac gacgtggcct
     1141 cgcacgcgtc gggcatgcat tcgctgcagg cggtgcaggc gctatccact atcgatgccg
     1201 gcctcgcgcg attgcgcgac gggttgcacg cgcgcgggct ggacgaccgc gtggacctga
     1261 tcgtgctgtc cgaccacggc atggccgatg tgcccgccgc gaacctcgtc ttcctcgacg
     1321 acctgctgcc ggcggatgcg atgcaggtcg aatccgtcgg gcagatggcg tggctgaccg
     1381 cgaaaccggg ccgcgaggcg gaggtcgagc gtgcactggt gcgtcgccac gatcatttcg
     1441 aatgctggcg caaggccgat gcgccgcctg cctggcactt cggccgcaac ccgcgcatcc
     1501 cgccgatcat ctgccaggcc gacgatggct ggcgcgtgtg gctgcatcgc tatccggtct
     1561 cgaccgtgct gaagggcgag cacgggttcg cgccggagga cgtgggcatg cgcgcggcgt
     1621 tcactgcggt ggggccgtcc ttccgcaacg gcgtgcgcct gcccgcgttc gacaatgtcg
     1681 atgtgtatgc gttgctggcg cgcttgttgc ggatcacgcc tgcagcgaac gatggcgaca
     1741 tcgcgcccct gctgcccgca ttgaaggaca gccgatgatc ctgtgccggc gcatcgacag
     1801 cccggtcggg ccactgatgc tggcggccga cgacagcggc ctccgccaca tcgaattccg
     1861 cgacaaccgc catccggtca atcgcggcga ctggcacggc ggcgacaacg cgatcctgca
     1921 ggccaccgag gcgcagctcg ccgagtattt cgccggcacg cggaccaggt tcgatctccc
     1981 ccttgcaccg cgcggcaccg agttccagtt gcgcgtgtgg caggcgctgg cgcggatccc
     2041 gcacggatcc acctcgacct atgccgagca ggcgcgcgcc atcggcagtc ccgatgcggt
     2101 gcgcgcggtg ggggcggcga acggacgcaa tccgttgccg atcgtgctgc cctgccatcg
     2161 ggtgatcggt gccgatggcg cgctggtcgg cttcggtggc ggcctgccga tcaaggagtt
     2221 cctgctgcgg ctggaaggcg cgttgccggc acgacaggcc ggattgttct gaatgcgggt
     2281 gcctgcttag cctcagcacc aaccggtcag gactatcgaa gcagttgctt catcgcatcg
     2341 cgataccgcg tggcgatcgc gtcgcggtcg cgatgccggc cgtcctcgac cacgcgcacg
     2401 ccggcgactt cgacatcgcg caccagcggg cgattgccgc tgaacagcca gcggtcgatg
     2461 acatcgccat ccgtcgcgcc ggcgaggatc ggcgcgtccg catccagcac gattcgatcc
     2521 ccagccgcga agccggtgct gtccgcagcg cttgcatggg cgccatccag caacgtggcg
     2581 ccaacgctcg gcatgcgcat gctcgaagcg atgttgcgac gctgcacgcg caggcgttgt
     2641 ccatattcca gccagcgcag ttcctcgacc ggatcgaccg agctgttcga atccgaaccc
     2701 acgccccaac gcccgcccgc atcgagatag tcccgcagcg ggaataggcc atcgccgaga
     2761 ttcgcctcgg tgctggtgca gatcgccacg gtggcaccac tgcccgcaat gtcctgcact
     2821 tcgtcatcgt ccagatgggt cgcatgcacc agcgtccagc gctcatccac cggcacgctg
     2881 tccagcagcc attgcaccgg ccgcgcgccg cgcagggcga tgcattcctc gacctcgggc
     2941 agttgttcgg cgatgtggat gtggatgcgc gacgccgccg gcagtgcggt caggacttca
     3001 tgcatcgggt cggacggcac cgcgcgcagg ctgtgcaggg cgcagccgat gcgnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncatgcat cgggtcggac ggcaccgcgc
     3181 gcaggctgtg cagggcgcag ccgatgcgaa cggtgtcgct ttcgctggcc cgcaaggtct
     3241 cgagcaagcg caggtaggcg tcgacggaat ggccgaagcg ctgctggcgc gccgacagcg
     3301 gcctgccatc gaacccgccg gtcatgtaca gcaccggcag cagggtgatg cggatgccgg
     3361 tgtcgcgcgc ggcacgcagc aacgcctgcg acatcgcatc ggcctgcgca tacggcgtgc
     3421 catccggcgc gtggtgcagg tagtggaatt cgcagaccgt ggtgtagccg gcttcgagca
     3481 tttccgcgta cagctgcgag gcgacggcgt gcagcaggtc gggatcgaag cgcgcggcca
     3541 tcgcgtacat ggtttcgcgc caggtccaga acgaatcggc aggatcggtc tggcgttcgg
     3601 ccaggccggc catcgcccgc tggaaagcat gcgaatgcag gttggcgatg ccgggcaagg
     3661 cccagccgcg cggggtgcgg gtggcgatgg cagtcgtcgg cgtgtgcatc ggcgcattgt
     3721 cgcgcaaacc cgccacgggc tgcggtaggc tttgcacatg gacggcatgc tggtgatcgg
     3781 aatgatgttc gcggccatgg ccctggccgt ggtgctgatc ctgcacctgc gtcgtcgcta
     3841 cggctccggc aacggcggcg atggcggcta cggtggtgag agcggccatt cgcaccacga
     3901 ccatgacggc ggctgcgatg gcggtggcga cggaggcggt ggtgactgag cgctgggatg
     3961 gcctgctgct gggcccgacc ctggcgaccc tcgacaccgg cacgggctac ggcctggtcg
     4021 aagacggcgc actgggctgg aacgatggcc tgctcaccta tgtcggcccg cgcagtggat
     4081 tgcccggcga tgcgccatcg cttgccgacg acgtgatcga agccgaaggg ctggtcacgc
     4141 cgggcctggt cgattgccac acgcacctgg tgttcgccgg cgatcgcgcg agcgaattcg
     4201 agttgcgcct gcagggcgcg agttacgagg acatcgcgcg cagcggcggc ggcatcctct
     4261 ccagcgtgcg cgccgtacgc gaggccagcg aagacgaact cctggcgcag tcgttgccac
     4321 gcgcacgcgc attgatcgcc gacggtgcga ccacgctgga gatcaagtcg ggctatggcc
     4381 tggacctggc caacgaaggc aagatgttgc gggtcgcgcg gcgcatcggc gaaaccctcg
     4441 gcatctgcgt gcgcaccacc ttcctcggcg cgcatgcgct gccgcccgaa tatcgcgacg
     4501 atgccgacgg ctacatcgac gccgccatcg gctggctgca cgcgctgcat gccgaggggt
     4561 tggtggatgc cgtggatgcg ttctgcgagc gcatcggctt ctcatccgcg cagacgcgac
     4621 ggatgttcga ggccgcacgc ggacttggcc tgccggtgaa actgcatgcc gaccagctca
     4681 gcgatggcgg tggcgcagcg ctggcggcgg aattcaatgg gttgtccgcc gaccacatcg
     4741 aatacacgga cgaaggcggc gtgcgcgcca tggccgacgc cggcacggtc gcggtgctgc
     4801 tgcccggtgc gttccacgtg ttgcgcgaaa cgaaactccc gccactggac ctgctgcgcg
     4861 aacacagcgt gccgatggcg gtggcgaccg actgcaatcc cggcacctcg ccgctgctgt
     4921 cgctgcgaca ggcgatgcag ctggcctgca cgcatttcaa gctgacgccg gaagaagcat
     4981 tgcgcggcac cacgatcaac gccgcgcgtg cactcggact gcacgatcgc ggcacgttgc
     5041 gcgtcggcct gcgtgccgac ttcgcgcact ggcgcgtcgc gcgtcccgcg gcgctctgct
     5101 actggctggg cgggcaactg ctcgacgcct cgtatgccgg cggtcgccgg ctcgtctgaa
     5161 tccctggaga ccttcatgcg ccatgctgcc ctcgccgctt cgatcctggt ttcgctggcc
     5221 cttgccgcac ccatcgcgca agcgcaggac gcggcgacca gactggcgca ggacgcgatc
     5281 atcgtggaca cgcacatcga tgcgcccggc atcctgatgg acaagtgggc
//
LOCUS       DAOOCX010000156         5317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_14531_length_5317_cov_8.454960, whole genome shotgun sequence.
ACCESSION   DAOOCX010000156 DAOOCX010000000
VERSION     DAOOCX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14531_length_5317_cov_8.454960"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PK399_11195"
     CDS             <1..989
                     /locus_tag="PK399_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HPW13726.1"
                     /translation="NRAADVNPINLLALALLSTPKHAMGEADLLAQIALSQTLLREVP
                     YAPHVTVTPHTPAQIVAHGEEIGTLLRTAHPLGDVLSVDDENAVLLSYFRNNVLHLFT
                     APAWIAVCFLNNRRMSRAQLQRLGRTLYPFLQAELFLPWDEDGFAERIDRTIDIFVRE
                     GLLQQVSDDDGGILARNTGQTDEVFRLRAIGHAMQQAFERYYIAISVLVKNGPGTLGA
                     GELESLCQLAAQRLSLLYAPAAPEFFDKGLFRGFIQELRALKLVWPDENSKLVFDDRL
                     KAWAKDARVVLGRELRHTIERVSPNSGRSTGEIPAVPEPPAAPPADQAPPAE"
     gene            complement(1042..2721)
                     /locus_tag="PK399_11200"
     CDS             complement(1042..2721)
                     /locus_tag="PK399_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13727.1"
                     /translation="MDMMAKRRVGSGLLWLVLAATGCWSGGAAAQTSAGAIPDGRGRV
                     VALAAGGYHTCALRSSGSVACWGSNWGGESTPPTKGYFTAVAAGLYHSCALRSDGQAV
                     CWGDNNYGQTSVPAGERFTAIAAGSTHSCGLRASGAMVCWGNNAAGQATPPTKQSFVA
                     LTAGVEHTCGLRADGAAMCWGASNNGATAIPFGETFTAISAGNFHTCGLRATGVGVCW
                     GNGLVASIAGSQVSTALAAGGVHTCTISQTGTAVCGGFNNYGQSSPPLVKGNRYTAMA
                     SGSYHTCALNQNGNVVCWGDNSYGQGNAPRGPFGMGAVASGYSHNCDLGADGTLVCAG
                     SNGSGESSPPAGSYADVGAGAAFSCARAASGSLSCWGFNDVNRATPPAGPFALMEIGS
                     AGGCALAASGVPACWGNNGNGQAMPALPPFVRAYRQVDLGAFHGCGIFDNGGGSCWGY
                     SGTSATSVPGGSWLAISGGEQFSCGLRSNGALACWGGGMPAPPTGGFKALASGSYHAC
                     AIRDSGELACWGSNAWGQANAPAGRYVHVSSGDKHSCAIRENGARVCWGTI"
     gene            complement(2848..3066)
                     /locus_tag="PK399_11205"
     CDS             complement(2848..3066)
                     /locus_tag="PK399_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002803587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HPW13728.1"
                     /translation="MNQPRDPAELSRMARRVVEMRQEHRDLDVAIERLAADIQADELA
                     LKRLKKRKLQLKDQIAWIENALIPDEPA"
     gene            3135..4031
                     /gene="ttcA"
                     /locus_tag="PK399_11210"
     CDS             3135..4031
                     /gene="ttcA"
                     /locus_tag="PK399_11210"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011270092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HPW13729.1"
                     /translation="MNAALPLPLAEPQPLALKRKHQADKLAKRLRHQVGKAIADFGMI
                     EDGDRVMVCLSGGKDSYAMLDVLLQLQKKAPVSFSITAVNLDQKQPDFPEHVLPTYLR
                     SIGVDFHIIEQDTYSVVSRVIPEGKTMCSLCSRLRRGALYTYAEQHGFTKIALGHHRD
                     DLVNTFFLNLFFHAKLSGMPPKLLSDDGKHVVIRPLAYVRESDIEAYAEVKQFPIIPC
                     NLCGSQENLQRAQVKKMLAQWEKDSPGRIETMSRALGDIRPSQLSDPTLFDFRSLGKR
                     GDAVLPDAHAWLAGTHEGDVDA"
     gene            4231..5136
                     /locus_tag="PK399_11215"
     CDS             4231..5136
                     /locus_tag="PK399_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HPW13730.1"
                     /translation="MFFRNLTLFRFPTSLDLSDLETRLSECALKPVGPLELSSRGFVS
                     PFGHHGEALSHAMHDALWVTVGGEDKLLPGAVVNDLLQKKLAAIEEQEGRKPGGRTRK
                     RLKDDLITELLPRAFVRPVRSDALLDTSLGVIAVDTSSRKNAESVVSEIRRALGSFPA
                     LPINAEVAPRAILTGWIAGDPLPEGLSLGDECELRDPTDTGAVVKVQRMDLSGDEIAR
                     HLESGKQATRLALVLDDHVSFVIGEDLVVRKFKLLDGAVDQLESTERDDVAAELDARF
                     ALMAGEFKRLFNVLEKAFKLSKADA"
BASE COUNT          842 a         1867 c         1774 g          834 t
ORIGIN      
        1 tcaaccgcgc cgccgacgtg aacccgatca acctgctggc gctggcgctg ctgtccacgc
       61 ccaagcacgc catgggcgaa gccgacctgc tggcgcagat cgcgctgtcg caaaccctgc
      121 tgcgcgaagt gccgtacgcc ccgcacgtca ccgtcacccc gcacacgccg gcgcagatcg
      181 tcgcccacgg cgaggagatc ggcacgctgt tgcgcacggc gcatccgctg ggcgacgtgc
      241 tcagcgtgga cgacgagaac gcggtgctgc tcagctactt ccgcaacaac gtgctgcacc
      301 tgttcaccgc gccggcatgg atcgcggtgt gcttcctcaa caaccggcgg atgtcgcgcg
      361 cgcaactgca acggctgggc cgcacgctct acccgttcct gcaggcggag ctgttcctgc
      421 cgtgggacga ggacggcttc gccgaacgca tcgaccgcac catcgacatc ttcgtgcgcg
      481 agggcctgct gcagcaggtc agcgacgacg atggcggcat cctggcccgc aacaccgggc
      541 agaccgacga agtgttccgc ctgcgcgcga tcggccatgc gatgcagcag gcgttcgagc
      601 gctactacat cgcgatttcg gtgctggtga agaacggtcc cggcacgctc ggcgccggcg
      661 aactggagag cctgtgccag ctggccgcgc aacgcctgtc gctgctgtac gccccggccg
      721 ccccggagtt cttcgacaag ggcctgttcc gcggcttcat ccaggagctg cgcgcgttga
      781 agctggtgtg gcccgacgag aacagcaagc tggtgttcga cgaccgcctg aaggcgtggg
      841 cgaaggacgc gcgggtcgtg ctgggccgcg aactgcgcca caccatcgaa cgggtcagcc
      901 cgaacagcgg gcgcagcacc ggcgagatcc cggccgtgcc ggagccaccc gccgcgccgc
      961 ctgccgacca ggcgccgccc gccgaatgaa ggacaggcga ggccgacgcc tcgcctgtcc
     1021 aggttcaaac cccggtccgg gtcagatcgt gccccagcac acccgcgcgc cgttctcgcg
     1081 gatcgcgcag ctgtgcttgt cgccgctgct gacgtgcaca tagcgacctg cgggcgcatt
     1141 cgcctggccc caggcattgc tgccccagca ggccagttca ccgctgtcgc ggatcgcgca
     1201 ggcgtggtag ctacccgagg ccagggcctt gaatccaccg gtgggcgggg ccggcatgcc
     1261 gccgccccag caggccagcg cgccgttcga ccgcaggccg cagctgaatt gctcgccgcc
     1321 gctgatcgcc agccagctgc cgcccggcac gctggtcgcg ctggtgccgc tgtagcccca
     1381 gcaggaaccg ccgccgttgt cgaagatccc gcacccgtgg aaggcgccca ggtcgacctg
     1441 gcggtacgcc cgcacgaacg gcggcaatgc gggcatggcc tggccattgc cgttgttgcc
     1501 ccagcaggcg gggacaccac tggcggccag tgcgcagccg ccggcggacc cgatctccat
     1561 caaggcgaag gggccggccg gaggcgtcgc ccggttcacg tcgttgaatc cccagcagga
     1621 caggctgccg ctggcggcgc gtgcgcaact gaaggcggcg cctgcgccga catccgcata
     1681 gctgcccgcg ggcgggctgg attcgccgct gccgttgctg cccgcacaga ccagggtgcc
     1741 gtccgcgccc aggtcgcagt tgtgggaata ccccgaggcc accgcgccca tgccgaatgg
     1801 accgcgtggc gcattgccct ggccgtagct gttgtcgccc cagcagacca cgttgccgtt
     1861 ctggttgagc gcgcaggtgt ggtaggagcc gctcgccatc gcggtgtaac ggtttccctt
     1921 caccagcggc ggactggatt ggccgtagtt gttgaagccg ccgcagaccg cggtgccggt
     1981 ctggctgatc gtgcaggtat gcacgccgcc cgcggccagc gcagtggaga cctggctacc
     2041 ggcgatgctg gcgaccaggc cgttgcccca gcacacaccc acgccggtgg cgcgcaggcc
     2101 gcaggtatgg aagttacctg ccgagatcgc cgtgaacgtc tcgccgaacg ggatcgcggt
     2161 ggcaccgttg ttgctcgcgc cccagcacat cgccgcaccg tccgcccgca gaccgcaggt
     2221 atgttcgacg ccggcggtca aggccacgaa gctctgcttg gtcggcggcg tggcttggcc
     2281 tgcggcgttg ttgccccagc acaccatcgc gccgctggca cgcaagccgc agctgtgggt
     2341 gctgccagcc gcgatcgcag tgaatcgttc accggccggg accgacgtct gtccgtagtt
     2401 gttgtcgccc caacacaccg cctggccatc cgaacgcagg gcgcagctgt ggtagaggcc
     2461 ggccgccacg gcggtgaaat accccttggt cggcggcgtg ctctcgccgc cccagttgct
     2521 gccccagcac gccaccgaac cgctgctgcg caatgcgcac gtgtggtacc cacccgccgc
     2581 cagcgccacc acccgcccgc gcccgtccgg gattgccccc gcgctggttt gcgccgctgc
     2641 cccgcccgac cagcagcccg tcgccgccag taccagccac aacaggccgc tgccgacccg
     2701 ccgcttcgcc atcatgtcca tcgccccgtc ctcgccgccg gcaccacgcc ggctccacac
     2761 cctctatcgg gattcggaag cgaatgtggt catggggtcg gagcgttttc gtcggccctg
     2821 cgggacccac gtgcggtgcg ggtgccgtca ggcgggttcg tccgggatca aggcattctc
     2881 gatccaggcg atctggtcct tgagctgcaa cttgcgcttc ttcaggcgtt tcagcgccag
     2941 ctcgtcggcc tggatgtcgg cggccaggcg ttcgatggcg acgtccaggt cgcgatgctc
     3001 ctggcgcatc tcgaccacgc gtcgtgccat gcgggacagt tcggcgggat cgcgcggctg
     3061 gttcatcggc cgagcataac ccggcacgac gcgggcggca gcgctgtcgc cgcgacgggt
     3121 aaaatggcga cctcatgaat gccgccctcc ccctcccgct ggccgaaccg caaccgctcg
     3181 cgctcaagcg caagcaccag gcggacaagc tggccaagcg cctgcgccac caggtgggca
     3241 aggcgatcgc cgacttcggc atgatcgagg acggcgacag ggtgatggtc tgcctgtccg
     3301 gcggcaagga cagctacgcg atgctggatg tcctgctgca actgcagaag aaggcgccgg
     3361 tgtcgttctc gatcaccgcg gtgaacctgg accagaagca gccggatttc cccgagcacg
     3421 tgctgcccac ctacctgcgt tcgatcggcg tggacttcca catcatcgag caggacacct
     3481 attcggtcgt cagccgggtc atcccggagg gcaagacgat gtgctcgctg tgttcgcggc
     3541 tgcggcgcgg cgcgctgtac acctacgccg agcagcacgg cttcaccaag atcgcgctgg
     3601 gccatcaccg cgacgacctg gtcaacacct tcttcctcaa cctgttcttc cacgccaagc
     3661 tgtccggcat gccgccgaaa ttgctgtcgg acgacggcaa gcacgtggtg atccgcccgc
     3721 tggcctacgt gcgcgaaagc gacatcgagg cctatgccga ggtgaagcag ttcccgatca
     3781 tcccctgcaa cctgtgcggc tcgcaggaga acctgcagcg cgcgcaggtg aagaagatgc
     3841 tggcgcagtg ggagaaggat tcgccgggac gcatcgagac catgtcgcgc gcgctgggcg
     3901 acatccgtcc ctcgcaactc tccgacccga ccctgttcga cttccgctcg ctcggcaagc
     3961 gcggcgatgc cgtgttgccc gatgcgcatg cgtggttggc aggaacccac gaaggcgatg
     4021 tggatgcctg aggcttgacg cgaccatcgg cgacatgggt tcggcacact gtgccggacg
     4081 ggtgaaccac ggatggttcg ccccggcctt gccggttgcg caggaatgcg caaccgagca
     4141 agtcgccgat gagcctccat ggatggattc acggcgtgtc gcggcaagct tctggcgtcc
     4201 gcagcgcctc ccccgatcac aggatccttg atgttctttc gcaatctcac gctgttccgt
     4261 ttcccgacgt cgctcgacct gtccgacctc gaaacccgcc tttccgaatg cgcgctcaag
     4321 ccggtcggcc cgctggaact ttcatcgcgc ggcttcgtgt cgccgttcgg ccaccacggc
     4381 gaggcgctca gccacgccat gcatgacgcg ttgtgggtca ccgtgggcgg cgaggacaaa
     4441 ttgctgcccg gcgcggtggt caacgacctg ctgcagaaga agctggcggc gatcgaggaa
     4501 caggagggcc gcaagccggg cggccgcacg cgcaagcgcc tcaaggacga cctcatcacc
     4561 gagctgctgc cgcgcgcgtt cgtgcggccg gtgcgcagcg atgcgctgct cgacacctcg
     4621 cttggcgtca tcgcggtgga tacctcgtcg cgcaagaacg cggaatcggt ggtcagcgaa
     4681 atccgccgtg cattgggcag cttcccggcg ctgccgatca atgccgaagt cgcgccgcgc
     4741 gccatcctca ccggctggat cgccggcgac ccgctgccgg aaggcctgag cctgggcgac
     4801 gaatgcgaac tgcgcgatcc caccgacacc ggcgcggtgg tcaaggtgca gcgcatggat
     4861 ctgtccgggg acgaaatcgc ccggcacctg gagtccggca agcaggcgac ccggctcgcg
     4921 ctggtgctgg acgaccacgt cagcttcgtc atcggcgaag acctggtcgt gcgcaagttc
     4981 aagctgctgg atggtgcggt cgaccagctc gaatcgaccg agcgcgacga cgtggcggcc
     5041 gaactcgatg cgcgcttcgc gctgatggcc ggcgagttca agcgcctgtt caacgtgctg
     5101 gaaaaggcct tcaagctcag caaggccgat gcctgaactg gccggcggcg gccattgcgg
     5161 acgtagactg ggctggttgc atccggcaac cgcatcgggg aatgacatgg gcaagacact
     5221 gatggtgttg ctgcttggcg tgctcgcctg cgcgggcgcg atggcgcagt cgcgcggcga
     5281 ctgggtactc gggcaatggc agggcggcgc gtactgg
//
LOCUS       DAOOCX010000157         5231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_14871_length_5231_cov_8.280912, whole genome shotgun sequence.
ACCESSION   DAOOCX010000157 DAOOCX010000000
VERSION     DAOOCX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14871_length_5231_cov_8.280912"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PK399_11220"
     CDS             <1..833
                     /locus_tag="PK399_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010380519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPW13731.1"
                     /translation="QLGFKVAALGALVDGLDARRDIPQIEFITGDAHVALVFRHLQPL
                     SDADKARLDAFGTEHGFTMFLQPGGNDSVHPLSGEAPQLSFRLPQWDIELLFRPLDFI
                     QVNAKLNEAMIARALALLDVRPGERVLDLFCGLGNFTLPLARASGDGDVVGVEGEAGL
                     VQRARANAAHNGLTNVHFHAADLTQDLASQPWLKAGFDRLLLDPARSGAIDVLKQLPL
                     KGLKRIVYVSCHPGSLARDAGYLVNEAGWTLREAGVMDMFPHTAHVESIAVFDPPKKR
                     "
     gene            845..1372
                     /locus_tag="PK399_11225"
     CDS             845..1372
                     /locus_tag="PK399_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPW13732.1"
                     /translation="MGIEIERKFLLADEAWRAAAHKVVPMAQGYLNDLAMVDSGAMQT
                     SVRVRIEGESAFLNIKSREAGPSRQEFEYGIPLADARALLALCVGGRIDKHRHYVTHA
                     GHLWEIDEFHGDNAGLVVAEIELGGVDETFARPAWLGAEATHAQRYYNLALASRPYAQ
                     WRDDERLALDLNEGH"
     gene            1373..2425
                     /gene="nagZ"
                     /locus_tag="PK399_11230"
     CDS             1373..2425
                     /gene="nagZ"
                     /locus_tag="PK399_11230"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPW13733.1"
                     /translation="MLQIGVSGHELTAQERDWLQHDACIGVILFARNFASRAQVAELT
                     QAIREATPRPQLISVDQEGGRVQRFREGYTALPALQGFDALYREDPTAALALAREHAW
                     LMASEIRATGIDLSFAPVIDLGRGNRAIGNRAFSADPDVVAAFASTYVAGMHEAGMAA
                     TLKHFPGHGSIPEDTHFDVAVDDRSLEAMRATDLVPFVAGIAAGADAVMMAHITYPQV
                     APEPAGYAPRWIGEILRGEMGFRGVVFSDDIGMAAAHSAGGVKARIDAHLDAGCDVVL
                     VASPALVEESLRGMTGRSSNTMALAGLMGRGALGWDGLLADGRRDDVQARLGRVRESV
                     VDAGATSGAINVGDVA"
     gene            2422..2979
                     /locus_tag="PK399_11235"
     CDS             2422..2979
                     /locus_tag="PK399_11235"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HPW13734.1"
                     /translation="MSGPKLADALATAHVVFDKATLEAAIVRMAGEIRPAYADGEVPL
                     YITVMNGGLPFAAQLAFALGELGLDLQFDYLHATRYRGQTTGSGLAWLHRPGTPMRGR
                     KVLLADDILDEGHTLLAVKQWCEDQGAAEVRIAVLAEKVHDRCVEGICADHIGVQVPD
                     AYVFGYGMDFHEQGRNLPAIYALGE"
     gene            3018..3767
                     /locus_tag="PK399_11240"
     CDS             3018..3767
                     /locus_tag="PK399_11240"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HPW13735.1"
                     /translation="MTIDLAVIGGTGVYALGELADVETHQPVTPYGAPSGPIRLGTYA
                     GKRVAFLARHGEGHSLPPHKINYRANLAALKAIGATRVLAMNTVGGITAQCGPRVLAC
                     PDQLIDYTWGRISTLCEEPGTEVLHVDFGDPYTPSLRVGVITAAKQAGVALVDAGCYG
                     ATQGPRLETKAEIARMRRDGCDLVGMTGMPEAGLARELGLDYACLAIVANWAAGAGPD
                     VDEVITLQDVLDNVAAAMDDAARLLRAMLAG"
     gene            3776..5032
                     /locus_tag="PK399_11245"
     CDS             3776..5032
                     /locus_tag="PK399_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW13736.1"
                     /translation="MTRRHVAIVGYGTGGQAAALALARHGHSVEVFERVPDPGPVGAG
                     FLLQPTGLQALWRLGLLDEACALGAPVARLHGETPEGRTVMDMHYADLDVRLSGVGMQ
                     RGALFAMLHEAIGLEGGIQLHAGHGITELDCERGRLRDAQGAWHGPFDVVVVADGSAS
                     SLRATVTGMRIDAPYPWGALWCLLPEGDWPWPAELRQRYHRARRMAGMLPVGTMPGDP
                     LRRLSFFWSLPVAQFDAWRAGGRDAWLGELHALWPRAREHMGDDFQVGCLARAAYRDA
                     IPTRWWRGRAMLLGDAAHAMSPQLGQGVNMALVDAVSLAHAFDAHGDLQVAFQDHARE
                     RRAHLAAYHRFSRWLTPLFQSDRDALARVRDLAFLPLGRLPGARGHMLRVLSGTQRGW
                     WGRHRLDDAFVAALQQALAVDSATPA"
     gene            5140..>5231
                     /locus_tag="PK399_11250"
     CDS             5140..>5231
                     /locus_tag="PK399_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPW13737.1"
                     /translation="MQTGTVKWFNDAKGFGFISPEDGSADVFVH"
BASE COUNT          780 a         1732 c         1855 g          864 t
ORIGIN      
        1 cgcaactcgg cttcaaggtc gccgcgctgg gcgcgctggt cgatggcctg gatgcgcggc
       61 gagacatccc gcagatcgag ttcatcaccg gcgacgcgca tgtggcgctg gtgttccggc
      121 atctgcagcc gctgtccgat gccgacaaag ccaggctgga tgccttcggc accgagcatg
      181 gcttcacgat gttcctgcaa cccggcggca acgactccgt gcatccgctg tcgggcgagg
      241 caccgcagct gtcgttccgc ttgccgcaat gggacatcga actgctgttc cgcccgctgg
      301 atttcatcca ggtcaatgcg aaactcaatg aggcgatgat cgcgcgcgcg ctggcgttgc
      361 tcgacgtgcg acccggcgag cgcgtgctcg acctgttctg cggcctcggc aacttcaccc
      421 tgccgctggc gcgcgcgtcg ggcgatggcg atgtcgtggg cgtggagggc gaggccgggc
      481 tggtgcaacg tgcccgcgcg aatgccgcgc acaacggcct caccaacgtg catttccatg
      541 ccgcggacct gacccaggat ctcgcatcgc aaccgtggct caaggccggc ttcgacaggc
      601 tgctgctgga tcccgcgcgt tcgggcgcga tcgatgtgct gaagcaactg ccgttgaaag
      661 gcttgaagag gatcgtctac gtcagctgcc acccgggctc gctggcccgc gacgccggct
      721 acctggtcaa cgaggccggc tggaccctgc gcgaggccgg cgtgatggac atgttcccgc
      781 ataccgcgca tgtcgaatcc atcgccgtgt tcgatccgcc gaagaagcgc tgaggcgcga
      841 cgcagtgggc atcgagatcg aacgcaagtt cctgctggcc gatgaagcgt ggcgcgccgc
      901 cgcgcacaag gtcgtgccga tggcgcaggg ctatctcaac gacctggcca tggtcgattc
      961 cggcgcgatg cagacctcgg tgcgcgtgcg catcgagggc gagtccgcct tcctcaacat
     1021 caagtcgcgg gaggccggcc cgagccgcca ggaattcgag tacgggatcc cgctggccga
     1081 tgcgcgtgcg ctgctggcgt tgtgcgtggg cggcaggatc gacaagcacc gccactacgt
     1141 cacccacgcc gggcatctgt gggaaatcga cgaattccat ggcgacaacg ccggtctggt
     1201 ggtggcggag atcgagctgg gaggcgtcga tgagacgttc gcccggcctg cctggctcgg
     1261 cgccgaagcg acccatgcgc agcgctacta caatctggcg ctggcctcgc gcccgtacgc
     1321 gcaatggcgc gacgacgagc gcctggcgct ggatctcaac gaaggacact agatgctgca
     1381 gatcggcgtt tccgggcacg aactcactgc gcaggagcgc gactggctgc agcacgacgc
     1441 ctgtatcggg gtgatcctgt tcgcgcgcaa cttcgccagc cgcgcgcagg tggcggaact
     1501 cactcaggcc attcgcgagg cgacgccgcg gccgcaattg atcagcgtgg accaggaagg
     1561 cgggcgcgtg cagcgtttcc gcgagggcta taccgcgttg ccggcactgc agggcttcga
     1621 tgcgttgtat cgggaagacc cgaccgctgc gctggcattg gcacgcgagc acgcctggct
     1681 gatggccagc gagatccgcg ccaccggcat cgacctcagc ttcgcgccgg tgatcgacct
     1741 ggggcgcggc aaccgcgcga tcggcaaccg cgcgttctcg gcggatccgg acgtcgtggc
     1801 ggcgttcgcc agcacatacg tcgcaggcat gcacgaagcc ggcatggccg ctacgctcaa
     1861 gcatttcccc gggcacggtt cgatcccgga ggacacccat ttcgatgtcg cggtggacga
     1921 ccgttcgctg gaggcgatgc gcgccaccga cctggtgccg ttcgtcgccg gcatcgccgc
     1981 cggcgccgat gcggtgatga tggcgcacat cacctatccg caggtcgcgc cggaacccgc
     2041 cggctacgcg ccgcgctgga tcggcgagat cctccgcggc gagatgggct tccgcggcgt
     2101 ggtgttctcc gacgacatcg gcatggccgc ggcccattcc gccggcgggg tgaaggcgcg
     2161 catcgatgcg cacctggatg ccggctgcga cgtggtgctg gtggccagcc cggcgctggt
     2221 cgaggaatcc ctgcgcggga tgaccggacg cagttcaaac acgatggcgc tggccggctt
     2281 gatggggcgc ggcgcgctgg gttgggatgg attgctggcc gatgggcgcc gcgacgatgt
     2341 gcaggcgcgg ctcggccgcg tgcgcgaatc ggtggtggat gccggtgcga cctcgggtgc
     2401 gatcaacgtg ggagacgtgg catgagcgga ccgaaactgg cggatgcatt ggcgaccgca
     2461 cacgtggtct tcgacaaggc caccctggaa gcggcgatcg tgcgcatggc cggcgagatc
     2521 cgcccggcct acgcggacgg cgaggtgccg ctgtacatca cggtgatgaa cggtgggttg
     2581 ccgttcgcgg cgcaactcgc gttcgcgctt ggcgaactcg ggttggacct gcagttcgat
     2641 tacctgcatg ccacccggta tcgcggccag accaccggct ccggcctggc ctggctgcat
     2701 cgcccgggca cgccgatgcg cggacgcaag gtcctgctgg ccgacgacat cctcgacgag
     2761 ggccacaccc tgctggcggt gaagcagtgg tgcgaagacc agggcgcagc cgaggtgcgc
     2821 atcgccgtgc tggccgagaa agtccacgac cgctgcgtcg aaggcatctg tgccgatcat
     2881 atcggcgtgc aggtgccgga tgcctacgtg ttcggctacg ggatggattt ccacgagcag
     2941 ggtcgcaacc tgccggcgat ctatgcgctt ggtgagtaac gttggcctcg cctcctactg
     3001 tctctctgga atcaggcatg accattgatc tcgccgtcat cggcggtacc ggcgtctacg
     3061 cgctcggcga actggccgat gtcgaaaccc accagccagt gacgccgtac ggtgctcctt
     3121 cggggccgat ccggctcggc acgtatgccg gcaagcgcgt ggcattcctg gcgcggcatg
     3181 gcgaaggcca ttcgctgccc ccgcacaaga tcaactatcg ggccaacctg gctgcgctga
     3241 aggcgatcgg cgcgacgcgc gtactggcga tgaacaccgt cggcggcatc accgcgcaat
     3301 gcggaccgcg cgtgctggcc tgcccggacc agctgatcga ctacacgtgg ggacgcattt
     3361 ccaccctctg cgaggaaccc ggcaccgaag tcctgcacgt cgatttcggt gatccctaca
     3421 cgccttcgct gcgcgtcggc gtcatcacgg ctgcgaagca ggccggcgtg gccctggtcg
     3481 atgccggttg ctatggcgcc acccagggtc cgcgcctgga aaccaaggcc gaaatcgcgc
     3541 gcatgcgtcg cgacggctgt gacctggtcg gcatgaccgg catgcccgag gccggcctgg
     3601 cccgcgaact cggcctggac tatgcgtgcc tggcgatcgt cgcgaactgg gcggcgggcg
     3661 cgggccccga tgtcgatgaa gtgatcaccc tgcaggacgt gctggacaac gtggccgcgg
     3721 cgatggacga cgcggcccgg ctgttgcggg cgatgctggc cggctgagcc gcgcgatgac
     3781 gcgtcggcac gtcgccatcg tcggctacgg caccggcgga caggctgccg cgctggcact
     3841 ggcgcgccac ggacattcgg tcgaagtctt cgagcgcgtg ccggatcccg gcccggtcgg
     3901 cgccggcttc ctgttgcaac ccaccggcct ccaggccctg tggcgactgg ggctgctcga
     3961 cgaggcctgc gcattgggtg cgccggtggc gcgcctccat ggcgagacgc ccgaaggccg
     4021 caccgtcatg gacatgcact acgcggacct ggacgtgcga ctgtccggcg tgggcatgca
     4081 gcgtggcgcg ttgttcgcca tgttgcatga ggcgatcggc cttgaaggcg gcatccaact
     4141 gcatgccggc catgggatca cggaactcga ctgcgagcgc ggtcgcctgc gcgacgcgca
     4201 gggcgcgtgg cacgggccgt tcgatgtggt ggtggttgcg gatggctcgg cgtcgtcgct
     4261 gcgcgccact gtcaccggca tgcgcatcga cgccccgtat ccctggggtg cgctgtggtg
     4321 cctgttgccg gaaggcgatt ggccatggcc ggccgagctg cgccagcgtt accatcgcgc
     4381 gcgccggatg gccgggatgc tgccggtggg aaccatgccc ggcgatccgt tgcgccgatt
     4441 gagcttcttc tggagcctgc cggtcgcgca gttcgacgcc tggcgcgctg gcggccgcga
     4501 tgcgtggctc ggcgaactgc acgcgctatg gccgcgtgcg cgcgaacaca tgggcgacga
     4561 tttccaggtc ggctgcctgg cacgcgcggc gtatcgcgat gcgatcccca cgcgctggtg
     4621 gcgcggccgc gcgatgctgc tcggcgatgc ggcgcatgcg atgagcccgc agctggggca
     4681 gggggtgaac atggcgctgg tcgacgcggt ctcgctggcg cacgcgttcg atgcgcatgg
     4741 cgacctgcag gtggccttcc aggatcacgc gcgcgaacgg cgtgcgcacc tcgcggccta
     4801 ccaccgcttc agtcgctggt tgaccccgtt gttccagtcc gatcgcgatg cgctggcgcg
     4861 cgtgcgcgac ctcgccttcc tgccgctggg tcgactgccc ggtgcgcgtg ggcacatgct
     4921 gcgcgtgctc tccggcaccc agcggggatg gtggggacgc catcgactgg acgatgcctt
     4981 cgtggcggcc ctgcaacagg cgctggcggt cgacagcgcc acgcccgcct gaggccgcgc
     5041 tcggttgcac gaggacgcgc ttgcggcata ctcgcgcggt gcttccgcca ggcggcggcg
     5101 cacgttcctg caaccgctcg gcaagaggtc attcaatcca tgcaaaccgg caccgtgaag
     5161 tggttcaacg acgccaaggg cttcggcttc atctcgcccg aggatggcag tgcagacgtc
     5221 ttcgtgcatt t
//
LOCUS       DAOOCX010000158         5190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_15033_length_5190_cov_7.039922, whole genome shotgun sequence.
ACCESSION   DAOOCX010000158 DAOOCX010000000
VERSION     DAOOCX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15033_length_5190_cov_7.039922"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..785)
                     /locus_tag="PK399_11255"
     CDS             complement(141..785)
                     /locus_tag="PK399_11255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPW13738.1"
                     /translation="MNFDFTTLAFLAFAATAAVAALATITSRNPVHAALWLVLTFFSV
                     ACTWIVGGAEFLGIALVLVYVGAVMVLFLFVVMMLDIDLAPLREGYVKYLPVGLVVAV
                     LMLVQMLSLIGVRAASAMPLADNAATGADVPNTTWLARALFTDFMLPFEVAAVILTVA
                     VVAAVMLTLRRRGGTKHQDPSAQSRVKASDRVRMVKMKAETPAPPAAAEGEGTP"
     gene            complement(799..1287)
                     /gene="nuoI"
                     /locus_tag="PK399_11260"
     CDS             complement(799..1287)
                     /gene="nuoI"
                     /locus_tag="PK399_11260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HPW13739.1"
                     /translation="MGKIVSYFKSLLLLELLGGLWLTLKYLFKPKYTLMYPMEKTPQS
                     PRFRGVHALRRYANGEERCIACKLCEAVCPALAITIDSEQRADGTRRTTRYDIDLFKC
                     IYCGFCEESCPVDSIVETHVHEYHFDKRGQNILTKPQLLAIGDRLEAEIAERRAADAP
                     FR"
     gene            complement(1291..2379)
                     /gene="nuoH"
                     /locus_tag="PK399_11265"
     CDS             complement(1291..2379)
                     /gene="nuoH"
                     /locus_tag="PK399_11265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010375869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HPW13740.1"
                     /translation="MNPLMTHAVEPFRDFLLSLGTIGMLVWIVLKILAIAVPVIVSVA
                     FYVVWERKLIGWMHVRHGPMYVGWGVLQAFADVFKLLFKEVLQPAVAHPLLFRLAPLI
                     ALVPAFAAWAVVPFDAQVVLSNANAGLLYLLAMTSMGVYGVILAGWASNSKYAFLGAM
                     RGAAQVVSYEIAMGFALVGVLIAAGSLNLSDIVNAQGGNGGFTEWFMWPLLPLFVVYF
                     ISGVAETNRAPFDVVEGESEIVAGHMVEYSGSQFALFFLAEYANMILVSFLTATFFVG
                     GWLSPIPAAFIPDVPVLSTVLGDGWWWLLAKVFFFASCFIWFRASFPRYRYDQIMRLG
                     WKVFIPITIVWIFVTALLVFSGILQAGQ"
     gene            complement(2409..4643)
                     /gene="nuoG"
                     /locus_tag="PK399_11270"
     CDS             complement(2409..4643)
                     /gene="nuoG"
                     /locus_tag="PK399_11270"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HPW13741.1"
                     /translation="MSAQPVNPNLPPDHVTVIIDGVELAAPKGSMIIHAADKAGIPIP
                     RFCYHEKLPIAANCRMCLVDTEVGGRSAPKPSPACATPVMDGLKVFTRNEKALKAQRN
                     VMEFLLINHPLDCPICDQGGECELQDLSLGYGRSVSRFVERKRVVADEDMGPLVATEM
                     TRCIQCTRCVRFTADIAGTYELGGMQRGENLQIGTYDGKPLTTELSGNVIDVCPVGAL
                     TNKVFRFKARPWELTAKPSLGYHDALGSNLFHHVRRGEILRSVPRDNEAVNECWLSDR
                     DRYAHEGLYAADRATVPLIRDGENFREASWDEALAKAARILRDNAADDLGILVHPSTS
                     NEEGALLARLAEALGTGNLDHRIGQLDLSDGAAAEAFAMPVAEIEQADAIVIIGSNLR
                     HEVPLLHQRLRKAVKRGAKVHVVNPIDFDVAFAVASKQIVVPSQLADALGKVDLGDAA
                     TVAVIVGGVAENSMHAAAIRKAATDFAASKGARLCRIPQGANALGLSRHGVLPASRDA
                     QTMLREARAGYLIYGIEPGLDFADQLVALKALGGAQVVAFSHFACQSTRRVADVILPI
                     GALPEIDATLTNLDGVAQMTSAAGKLPGQAHAGWRVLRALADELKLDGFGYTDLAGLR
                     AGVAPKSVQAAKGSAPAIAGTGLEVVASQAIYRVDGVTRRAEALQAHPLNAGPRVLLN
                     PADAATAGLVDAAMAKLSTANGTATLQVVVDDRVAPGCAWIETGHGATAPLAASAKVE
                     VVRA"
     gene            complement(4640..>5190)
                     /locus_tag="PK399_11275"
     CDS             complement(4640..>5190)
                     /locus_tag="PK399_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPW13742.1"
                     /translation="IRLGTSFADLLALCGGMKPGRTLKGVIPGGSSMPVLPGEVMMGL
                     TMDYDAIQKAGSGLGSGAVVVMDDTTCMVRACRRISRFYWKESCGQCTPCREGTGWMH
                     RMLDRICNFQATLDDLQMLRAAAGQIEGHTICAFGEAAAWPVQGFLRHYWHEFEYAIV
                     NGRFYVDDERAGTLVKPVEAAA"
BASE COUNT          954 a         1749 c         1703 g          784 t
ORIGIN      
        1 cgagagcagc agcagctcca gcgacatcag cagcatgatc acgttcttcc ggttgaggaa
       61 gatgccggcc acgctgatgc agaacagcac cgcgcccagg gtgaggaagt ggcccagggt
      121 gagcgccgag aagacgttgc tcatggcgtg ccctcccctt cagccgccgc cggcggcgcg
      181 ggagtttcgg ctttcatctt caccatgcgc acgcgatcgg aggccttgac ccgggactgc
      241 gccgacggat cctggtgctt ggtgccacca cgacgacgca gggtcagcat caccgccgcg
      301 accaccgcca ccgtcaggat caccgcggca acctcgaacg gaagcatgaa gtcggtgaac
      361 agcgcccgtg ccaaccaggt cgtgttcggc acgtcggcgc cggtcgcggc attgtctgcc
      421 aacggcatcg cggacgcggc acgcacgccg atcagggaca gcatctgcac cagcatcagc
      481 accgccacca ccagcccgac cggcaggtac ttgacgtaac cctcgcgcaa cggcgccagg
      541 tcgatgtcca gcatcatcac tacgaacagg aacagcacca tcaccgcgcc cacgtagacc
      601 agcaccagcg cgatgcccag gaactcggcg ccacccacga tccaggtgca ggccaccgag
      661 aagaaggtca gcaccagcca caacgcggca tgcaccggat tgcgcgaggt gatcgtggcc
      721 agcgcggcga ccgccgcggt ggcggcgaag gccaggaacg ccaaggtcgt gaaatcgaaa
      781 ttcatcagtc tctcaacctc agcggaacgg cgcgtcggcg gcgcggcgct cggcgatctc
      841 cgcctccagg cggtcgccga tggccagcaa ctgcggcttg gtcaggatgt tctggccgcg
      901 cttgtcgaag tggtattcgt gcacgtgggt ctcgacgatc gagtccaccg ggcaggattc
      961 ctcgcagaag ccgcagtaga tgcacttgaa caggtcgatg tcgtagcgcg tggtgcggcg
     1021 ggtgccgtcg gcacgctgtt cggaatcgat ggtgatcgcc agcgccgggc acaccgcctc
     1081 gcacagcttg caggcgatgc agcgttcctc gccattggcg tagcggcgca gcgcatgcac
     1141 gccgcggaag cgcggcgact gtggcgtctt ctccatcggg tacatcagcg tgtacttcgg
     1201 cttgaacagg tacttcaggg tcagccacag gccgccaagc agttccagca ggagcaggct
     1261 cttgaagtag gaaacgattt tacccatggc ttactgcccc gcctgcagga tgccggagaa
     1321 caccagcaac gcggtcacga agatccagac gatggtgatc gggatgaaca ccttccagcc
     1381 cagccgcatg atctggtcgt agcggtagcg cgggaacgaa gcgcggaacc agatgaagca
     1441 actggcgaag aagaacacct tggccagcaa ccaccaccag ccatcgccga gcacggtcga
     1501 cagcaccggc acatccggga tgaaggccgc cgggatcggc gacagccagc caccgacgaa
     1561 gaaggtcgcg gtcaggaagc tgaccaggat catgttggcg tattcggcca ggaagaacag
     1621 cgcgaactgc gaaccggagt actcgaccat gtggccggca acgatttccg actcgccttc
     1681 caccacgtcg aacggcgcgc ggttggtttc ggccacgccg gagatgaaat acaccacgaa
     1741 cagcggcaac agcggccaca tgaaccattc ggtgaagccg ccattgccgc cctgcgcatt
     1801 gacgatgtcg ctgaggttga ggctgccggc ggcgatcagc acgccgacca gggcaaagcc
     1861 catcgcgatc tcgtagctca ccacctgcgc ggcgccgcgc attgcaccga ggaaggcgta
     1921 cttcgagttc gatgcccagc cggccaggat cacgccgtac acgcccatcg aggtcatcgc
     1981 cagcaggtac agcaggcccg cgttggcatt cgacagcacc acctgcgcat cgaacggcac
     2041 caccgcccag gcggcgaacg ccggcaccag cgcgatcagc ggcgcgagcc ggaacagcaa
     2101 gggatgcgcg acggccggct gcagcacttc cttgaacagc agcttgaaca cgtcggcgaa
     2161 ggcctgcagc acgccccagc cgacatacat cggtccatgg cgcacgtgca tccagccgat
     2221 cagcttgcgt tcccagacca cgtagaaggc gaccgagacg atcaccggca ccgcgatggc
     2281 caggatcttc aagacgatcc acaccagcat gccgatcgtg cccagcgaca gcaggaaatc
     2341 ccggaagggt tccaccgcgt gggtcatcag cgggttcaac gaagccagct cgagcgaggc
     2401 aatcgcattc atgcgcgcac gacctccacc ttggccgacg ccgcaagcgg ggcggtggcg
     2461 ccatggccgg tctcgatcca cgcacagccc ggcgcaacgc ggtcatcgac cacgacctgc
     2521 agggtcgcgg tgccgttcgc ggtggacagc ttcgccatcg ccgcgtcaac caggcccgca
     2581 gtcgcggcat cggccggatt gagcaacaca cgcgggcccg cattgagcgg atgtgcctgc
     2641 agggcctcgg cacgacgggt gaccccatcg acgcgataga tcgcctgcga ggccacgact
     2701 tcaagaccgg tgccggcgat ggcgggtgca ctgcccttcg cggcctgcac cgacttcggg
     2761 gcgacgcccg cacgcaggcc ggcaagatcg gtgtagccaa agccgtcgag cttcagttcg
     2821 tccgccagcg cacgcagcac gcgccagccg gcatgcgcct ggccgggcag cttgccagcc
     2881 gccgatgtca tctgcgcgac accatcgagg ttggtcagcg tggcatcgat ttcgggcaat
     2941 gcgccgatcg gcaggatcac gtcggcgacg cggcgggtgg actggcaggc gaagtggctg
     3001 aacgccacga cctgcgcgcc gcccagcgcc ttcaacgcga ccagttgatc ggcgaagtcc
     3061 aagcccggct cgatgccgta gatcaggtag cccgcgcgcg cctcgcgcaa catcgtttgc
     3121 gcatcgcgcg aggccggcag cacgccgtgt cgggacaatc cgagtgcatt cgcaccctgc
     3181 gggatgcggc agagcctggc gcccttcgat gcagcgaaat cggtcgccgc cttgcggatc
     3241 gcggcggcat gcatgctgtt ctcggccacg ccaccgacga tcaccgcgac ggtcgcggca
     3301 tcgcccaggt cgaccttgcc gagggcatcc gcaagctgcg atggcaccac gatctgcttg
     3361 gaggccaccg cgaaggcgac atcgaaatcg atcggattga ccacatgcac cttggcgccg
     3421 cgcttgaccg ccttgcgcag gcgttggtgc agcagcggga cttcatgacg caggttggag
     3481 ccgatgatga cgatcgcgtc ggcctgctcg atttccgcca caggcatcgc aaaggcttcc
     3541 gcggcggcgc catcggacag atccaactgg ccgatgcggt ggtcgaggtt gccggtgccc
     3601 agcgcctccg ccaggcgggc cagcagcgcg ccctcctcgt tcgaggtcga cggatgcacc
     3661 aggatgccga ggtcatcggc ggcgttgtcg cgcaggatcc gcgcggcctt ggccagcgct
     3721 tcgtcccacg acgcctcgcg gaaattttct ccgtcgcgaa tcagcggcac cgtcgcgcgg
     3781 tcggcggcgt acaggccttc atgcgcgtag cggtcgcgat ccgacagcca gcactcattc
     3841 accgcttcgt tgtcgcgcgg cacgctgcgc aggatctcgc cgcggcgcac gtggtggaac
     3901 aggttgctgc cgagcgcgtc gtggtagccc aaggacggct tggcggtcag ttcccatggg
     3961 cgcgccttga agcggaacac cttgttcgtg agcgcgccga ccgggcagac atcgatcacg
     4021 ttgccggaca gctcggtggt cagcggcttg ccgtcgtagg tgccgatctg caggttttcg
     4081 ccgcgctgca tgccgccgag ttcgtaggtg ccggcgatgt ccgcggtgaa gcgcacgcag
     4141 cgcgtgcact ggatgcagcg ggtcatctcg gtggcgacca gcggccccat gtcctcgtcg
     4201 gccaccacgc gcttgcgctc gacgaagcgg ctgaccgacc gcccatagcc cagcgacagg
     4261 tcctgcaatt cgcactcgcc gccctggtcg cagatcgggc agtccagcgg atggttgatc
     4321 agcaggaatt ccatcacgtt gcgctgggcc ttcagcgctt tctcattgcg ggtgaagacc
     4381 ttcagcccgt ccatcaccgg cgtggcgcag gccggcgacg gcttcggcgc ggaacgcccg
     4441 ccgacttcgg tatcgaccag gcacatgcgg cagttggccg cgatgggcag cttctcgtgg
     4501 tagcagaagc gcgggatcgg gatgccggcc ttgtcggcgg catggatgat catcgagccc
     4561 ttgggcgcgg ccagctccac cccgtcgatg atgacggtga catggtcggg cggcaggttg
     4621 ggattcaccg gttgcgcgct catgctgcgg cctccaccgg cttgaccaag gtgccggcgc
     4681 gctcgtcgtc gacataaaag cggccattga cgatggcgta ttcgaattcg tgccagtagt
     4741 ggcgcaggaa gccctgcacc ggccacgcgg cggcctcgcc gaaggcgcag atggtgtggc
     4801 cttcgatctg gccggcggcg gcgcgcagca tctgcaggtc gtccagggtc gcctggaagt
     4861 tgcagatgcg gtcgagcatg cggtgcatcc agccggtgcc ttcgcggcac ggggtgcact
     4921 ggccgcagct ttccttccag tagaagcgcg agatccggcg gcaggcacgc accatgcagg
     4981 tggtgtcgtc catcaccacg accgcgcccg agcccaggcc ggagccggcc ttctggatcg
     5041 cgtcgtagtc catggtcagg cccatcatca cctcgcccgg cagcaccggc atcgaggagc
     5101 cgcccgggat cacgcctttc agcgtgcgac cgggcttcat gccgccgcac agcgccagca
     5161 ggtcggcgaa cgaggtgccc aggcggattt
//
LOCUS       DAOOCX010000159         5129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_15251_length_5129_cov_9.011037, whole genome shotgun sequence.
ACCESSION   DAOOCX010000159 DAOOCX010000000
VERSION     DAOOCX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15251_length_5129_cov_9.011037"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /gene="glmS"
                     /locus_tag="PK399_11280"
     CDS             complement(<1..1496)
                     /gene="glmS"
                     /locus_tag="PK399_11280"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPW13743.1"
                     /translation="MCGIVGAIANRDVVPLLVDGLKRLEYRGYDSAGIAVIDGESVRR
                     VRRTGRVAEMEAAAIAEGFDATLGIGHTRWATHGGVTEGNAHPHISHGDLALVHNGII
                     ENHEQQRDRLRALGYEFTSQTDTEVVAHLIHHHRAQGLSLLQALQKSVKELDGAYALA
                     VIDRRDPSTLVAARMGCPLLVGLGEGENFVASDVSAIVSATRKVIFLEEGDTAELDRD
                     GVRVFDADDVEVQRDVHLSDVSLASLELGPYRHFMQKEIHEQPRAIGDTIEGIVDAGG
                     FDPALFGANAADVLAGIEAVQILACGTSYYAGLAARYWLEDIAGIPCAVDIASEYRYR
                     KVVANPKQLIVTISQSGETLDTMEALKYAKSLGQERTLSICNVPESAIPRASKLVFYT
                     RAGAEIGVASTKAFTTQLVALFALTGVLAKLRGSIDDAQEAAYLDDLRHLPGSVQHAL
                     NLEPQVALWADKFAPRQHALFLGRGRHYPIALEGALKLKEISYIHAEA"
     gene            1671..2960
                     /locus_tag="PK399_11285"
     CDS             1671..2960
                     /locus_tag="PK399_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW13744.1"
                     /translation="MDTRNRIRDTAGQDQVRATPAHPKRRQWLVGGIAGVALLALVAW
                     VASGWLGGGRSFDASRLRIAEVKRGDLVRDLSADGRVIAANSPTLYAIAAGTVTLQVV
                     AGDKVRKGQALAVIDSPELRSKLVQEESTLAGMQAEASRALLDAQIMRANARKALDQA
                     TVERTAAMRDLERYQRAYDGGAVPQNDLARAQDEMKKAEIGLAAAGKDFELQSRGAGL
                     DARNKQLLAERQHAVVVEQRRQVEALTLRAPFDGQVGQVQVPQGTNVIANGPVLSVVD
                     LSRFEVEMKVPESFARDLAIGMPAQITSNGTPFAGEIAAVSPEVVNGEVTARVRFADG
                     KQPPGLRQNQRLSVRIVMDTRRDVLMVERGPFLEQEGGSTAYVVDGDNAVRQPIQAGA
                     SSLDNVEIVSGLKAGDRIVVSGTDQFDNADRVRISGN"
     gene            2965..3177
                     /locus_tag="PK399_11290"
     CDS             2965..3177
                     /locus_tag="PK399_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13745.1"
                     /translation="MNAFVALPDAFNWTPEQLAGAVPLRLSEAALFDAASQHGDLAAA
                     NAPRERLRIAHQYRAAAALPTRFRIS"
     gene            3207..3935
                     /locus_tag="PK399_11295"
     CDS             3207..3935
                     /locus_tag="PK399_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW13746.1"
                     /translation="MLDMRQVTKVYRTELVETHALRSLDLHVGEGEFVAVTGPSGSGK
                     TTFLNIAGLLETFTDGHFTLDGQDVRGLNDDARSKLRNQKIGFIFQGFNLIPDLNLFD
                     NCDVPLRYRGMPASERKLRIEDALGMVGLGSRIKHYPAELSGGQQQRAAIARALAGSP
                     RLLLADEPTGNLDSQMARSVMELLEDINQQGTTIIMVTHDPELAARAQRNVHIVDGMA
                     TDLSLEHGLARGAHAPADAAVAST"
     gene            3949..>5129
                     /locus_tag="PK399_11300"
     CDS             3949..>5129
                     /locus_tag="PK399_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW13747.1"
                     /translation="MFGYYLNLAVRSFKRNKALTVLMVLAIALGIGASMTTLTVFHVL
                     SGDPIPEKSDQLFYVQLDPEGKQGYRPGEEPQTQLTRFDAETLLAQKRGLRQTMTNGG
                     NAVITPDKPGSAPELTETRYASSDFFAMFDVPLQYGRGWTAAEDEGKARVVVVSKELN
                     DKQFGGTDSTGRSLRIDGNEFRIIGVLDTWRLVPRFYDLYNDQYDTTEGLFLPFSTSR
                     DLKMGTQGNTNCWGDSGGDSRSLTAPCAWVQYWVELDTPAKAAEYKRYLVNYSDQQRK
                     AGRYERPTNVRLRNVMELMEFNEVVPGDVRLQLWLAFGFLLVCLVNTVGLLLAKFLRR
                     SGEIGVRRALGASRSEIFKQCLVEAGTVGVAGGVLGLLLALIGLWAVRQQPASYASLA
                     HL"
BASE COUNT          877 a         1760 c         1705 g          787 t
ORIGIN      
        1 taggcctcgg cgtggatgta ggagatttcc ttgagcttga gcgcgccttc cagcgcgatc
       61 gggtaatgcc ggccacggcc caggaacagc gcgtgctgcc tgggcgcgaa cttgtcggcc
      121 cacaacgcga cctgaggctc caggttgagt gcgtgctgca cgctgcccgg caggtggcgc
      181 aggtcgtcca ggtaggcggc ttcctgcgcg tcgtcgatgc tgccgcgcag tttcgccagc
      241 accccggtca gtgcgaacaa cgcgaccagc tgggtggtga aggccttggt cgaagccacg
      301 ccgatctcgg cgccggcgcg ggtgtagaac accagcttgc tggcgcgcgg gatcgcgctt
      361 tccggcacgt tgcagatcga caacgtgcgc tcctggccca gcgacttggc gtatttcagc
      421 gcttccatcg tgtccagggt ttcgccggac tgcgagatgg tgacgatcaa ctgcttcggg
      481 ttggccacga ccttgcgata gcgatactcg ctggcgatgt cgaccgcgca cgggatgccg
      541 gcgatgtcct ccagccagta gcgcgcggcc aggccggcgt agtagctggt gccgcaggcg
      601 aggatctgca ccgcttcgat gccagccagc acgtccgccg cattcgcgcc gaacaaggcc
      661 ggatcgaagc cgccggcatc gacgatgcct tcgatggtgt cgccgatcgc gcgcggctgc
      721 tcgtggattt ccttctgcat gaagtgccgg tacgggccga gttccagcga ggccagcgac
      781 acgtcggaca gatgcacgtc gcgctgcact tcgacatcgt cggcatcgaa cacgcgcacg
      841 ccatcgcgat cgagttcggc ggtgtcgcct tcttccagga agatcacctt gcgcgtcgcc
      901 gacacgatcg cggagacgtc ggacgcgacg aagttctcgc cttcgcccaa gccaaccagc
      961 aaggggcagc ccatgcgcgc ggcgaccagc gtggagggat cgcgacggtc gatcaccgcc
     1021 agcgcgtacg cgccgtcgag ttccttgacc gacttctgca acgcctgcag caggctcaac
     1081 ccctgcgcgc ggtggtggtg gatcaggtgg gcgacgactt cggtgtcggt ctgcgaggtg
     1141 aattcgtagc cgagcgcgcg caggcggtcg cgctgctgct cgtggttctc gatgatgccg
     1201 ttgtgcacca gcgcgaggtc gccgtggctg atgtgcggat gcgcgttgcc ttcggtcacc
     1261 ccgccatggg tggcccagcg cgtgtggccg atgccgagcg tcgcgtcgaa gccttcggcg
     1321 atcgccgcgg cttccatctc ggcgacgcgg ccggtacggc gcacgcggcg cacggactcg
     1381 ccatcgatca ccgcgatgcc ggcggaatcg tagccgcgat attccagccg cttgaggcca
     1441 tcgaccagca agggcaccac gtcacgattg gcgatggcac cgacgattcc gcacatgcag
     1501 gcaactcccg tttcgatgcg gaagtgtaac ggtgccggtg tccgcgcgga cgcggccggc
     1561 gtccggacgg acaccccgca tggcgcttcg ccctttctaa atcaatgact tgcggttggc
     1621 acgcttgttg cgctgtacac ggcatggaca gccaacagcc gaacgaccgc atggacaccc
     1681 gcaaccgcat ccgcgacacc gccggccagg accaggtccg cgccacgccc gcccacccca
     1741 aacgccgcca atggctggtt ggcgggatcg ccggggtggc cctgctggcg ctggtcgcct
     1801 gggtggcctc gggctggctg ggcggcgggc gttcgttcga tgcatcgcgc ctgcgcatcg
     1861 ccgaggtgaa gcgcggcgac ctggtccgcg acctcagcgc cgacggccgg gtcatcgccg
     1921 ccaacagccc gaccctgtac gcgatcgccg ctggcaccgt caccctgcag gtcgtcgccg
     1981 gcgacaaggt ccgcaagggc caggcgctgg cggtgatcga cagcccggaa ctgcgcagca
     2041 agctggtgca ggaggaatcc accctggccg gcatgcaggc cgaagccagc cgcgcgctgc
     2101 tggacgcgca gatcatgcgc gccaatgcgc gcaaggcgct ggaccaggcc acggtggaac
     2161 gcaccgcggc gatgcgcgac ctggagcgct accagcgcgc ctatgacggt ggcgcggtgc
     2221 cgcagaacga cctggcccgc gcgcaggacg agatgaagaa ggccgagatc ggcctggccg
     2281 ccgccggcaa ggatttcgaa ctgcagagcc gcggtgccgg gctggacgcg cgcaacaagc
     2341 aattgctggc cgagcgccag cacgcggtgg tggtcgaaca gcgccgccag gtcgaggcac
     2401 tgaccctgcg cgcgccgttc gatggccagg tcggccaggt gcaggtgccg cagggcacca
     2461 acgtgatcgc caacggcccg gtgctgagcg tggtcgacct gagccgcttc gaagtcgaaa
     2521 tgaaggtgcc ggaaagcttt gcccgcgacc tcgcgatcgg catgcccgcg cagatcacca
     2581 gcaatggcac gccgttcgcc ggcgagatcg ccgcggtctc gccggaagtg gtgaatggcg
     2641 aagtgaccgc gcgcgtgcgc ttcgccgacg gcaagcaacc gccgggcctg cgccagaacc
     2701 agcggctgtc ggtgcgcatc gtgatggata cccgtcgcga cgtgctgatg gtcgagcgtg
     2761 gcccgttcct ggaacaggaa ggcggcagca ccgcatacgt ggtcgacggc gacaacgccg
     2821 tgcggcagcc gatccaggcc ggcgcttcga gcctggacaa cgtggaaatc gtctcggggc
     2881 tcaaggccgg cgaccgcatc gtggtctcgg gcaccgatca gttcgacaac gcggaccgcg
     2941 tccgcatctc gggaaactaa tgccatgaac gctttcgtcg ccctccccga cgccttcaac
     3001 tggaccccgg aacaactcgc cggcgcggtg ccgctgcgcc tgtcggaggc tgcgctgttc
     3061 gacgccgcct cccagcacgg cgacctggcc gccgccaacg ccccgcgcga gcggctgcga
     3121 attgcccacc agtaccgcgc agcagccgca ctgcctacgc gcttccgcat ctcctgaccc
     3181 cacatccgca cacaaaggac atcgccatgc tcgacatgcg ccaagtcacc aaggtctacc
     3241 gcaccgaact cgtcgagacg catgcgctgc gctcgctcga cctgcatgtc ggcgaaggcg
     3301 aattcgtcgc cgtcaccggc ccgtcgggtt ccggcaagac cacgttcctc aatatcgcag
     3361 ggttgctgga gaccttcacg gacggccact tcaccctgga tgggcaggac gtgcgcgggc
     3421 tcaatgacga tgcgcgcagc aagctgcgca accagaagat cggcttcatc ttccagggct
     3481 tcaacctgat ccccgacctc aacctgttcg acaactgcga cgtgccgctg cgctaccgcg
     3541 gcatgccggc cagcgagcgc aagctgcgca tcgaggacgc gctgggcatg gtcgggctgg
     3601 gctcgcggat caagcactat ccagcggagc tctccggcgg ccagcaacaa cgcgcggcga
     3661 tcgcgcgcgc actcgcaggc agtccgcgcc tgctgctggc ggacgaaccc accggcaacc
     3721 tcgactcgca gatggcgcgc agcgtgatgg agctgctgga ggacatcaac cagcagggca
     3781 ccaccatcat catggtgacc cacgacccgg aactggcggc gcgcgcgcag cgcaacgttc
     3841 acatcgtcga cggcatggcc accgacctgt cgctcgaaca cgggctcgcg cgtggcgcgc
     3901 atgcgcctgc cgatgcagcc gttgccagca cctgacctgg agaccgccat gttcggttat
     3961 tacctcaacc tcgccgtgcg cagcttcaag cgcaacaagg cgctgaccgt gctcatggtg
     4021 ctggcgatcg cgctgggcat cggcgcgtcg atgaccacgc tcacggtctt ccacgtgctc
     4081 tccggcgatc ccatcccgga gaagagcgac cagttgttct acgtgcagtt ggatcccgag
     4141 ggcaagcaag gctatcgacc cggcgaagag ccgcaaaccc agctgacccg cttcgacgct
     4201 gaaaccctgc tggcgcaaaa gcgtggcctg cgccagacca tgaccaacgg cggcaacgca
     4261 gtgatcaccc cggacaagcc cggctcggca cccgagctca ccgaaacccg ctacgccagc
     4321 agcgatttct tcgcgatgtt cgacgtgccg ctgcagtacg gccgcggctg gacagctgcg
     4381 gaagacgagg gcaaggcgcg cgtggtggtg gtcagcaagg aactcaacga caagcagttc
     4441 ggcggcaccg acagcaccgg caggagcctg cgcatcgatg gcaacgagtt ccgcatcatc
     4501 ggagtactcg acacctggcg gctggtgccg cgcttctatg atctctacaa cgaccagtac
     4561 gacaccaccg aaggcctgtt cctgccgttc agcacctcgc gtgacctgaa gatgggcacc
     4621 cagggcaaca ccaactgctg gggcgattcc ggcggcgaca gccgttcgct cacggcgccc
     4681 tgcgcctggg tgcagtactg ggtcgagctc gacacgccgg cgaaggccgc ggagtacaag
     4741 cgctacctgg tcaactactc cgaccagcag cgcaaggccg ggcgatacga gcggccgacc
     4801 aacgtgcgcc tgcgcaacgt gatggaactg atggagttca acgaagtcgt gcccggcgac
     4861 gtgcgcctgc agctgtggct ggcgttcggc ttcctgctgg tctgcctggt caacacggtc
     4921 ggcctgttgc tggccaagtt cctgcgccgc agcggcgaga tcggcgtgcg ccgcgcactc
     4981 ggcgcgtcgc ggtcggagat cttcaagcag tgcctggtcg aagccggcac cgtcggcgtg
     5041 gccggcggcg tgctgggcct gctgctcgca ctgatcgggc tgtgggcggt gcgccagcaa
     5101 cctgccagtt atgcctcgct cgcccacct
//
LOCUS       DAOOCX010000160         5121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_15288_length_5121_cov_7.835965, whole genome shotgun sequence.
ACCESSION   DAOOCX010000160 DAOOCX010000000
VERSION     DAOOCX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15288_length_5121_cov_7.835965"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PK399_11305"
     CDS             <1..473
                     /locus_tag="PK399_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13748.1"
                     /translation="MENAGRERYLLVDDEGARIPLGDTEILALPAHFLHSVGNLSFFD
                     PVSRILFPGDVCSGLVGSGVPYAFVEDFEAYRPCMEGFHRRYMASNRALRLWVDMVRE
                     LAPEMIVPQHGMPFRGAAIKPLLDWLEGLQCGVDLMGPVNYRPNPQRPPVRVGY"
     gene            complement(499..2118)
                     /locus_tag="PK399_11310"
     CDS             complement(499..2118)
                     /locus_tag="PK399_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HPW13749.1"
                     /translation="MKTASRIDRSLIGQWLVGLALALLAGWLLRLQVQPWPTVTPLAQ
                     PRVFAGSALAAYAAFCGLIAWRARTPRVIASMDGEASWRVIYASQTGFALDLAERTAQ
                     ALRDAGQPACVHDINAIDLQVLDGAHCLFIASTTGEGDPPDHAMGFVKRVMTQPADLS
                     RTRYAVLALGDSEYAQFCAFGHELDEWLHRHGAQPMFDRVDVDNADPAALRHWQHRIG
                     VLSGHADQPDWTTPRHDRWTLAARHLLNPGSLGGEAWHIELQPPAGMALDWQAGDIAE
                     IGPRNAADDVAAFLARHALDGCHIVKHDRDDTPLSEILSRSRLPAMTDAVCNDTRALV
                     ASLQALPHREYSIASLPADGSLHLLVRLMQREDGTPGIGSGWLCRHAAIGDGIDLRIR
                     SNPNFHAPLASQPMILIGNGTGLAGLRAHLKARAAAGATRNWLLFGERNASADRLHGE
                     DLDAWQRAGVLERLDLVFSRDGHAQRYVQDALHANGDVLRTWVDQGAAVYVCGSLEGM
                     APGVDAALNEILGPSRMTALADAGRYRRDVY"
     gene            complement(2115..3098)
                     /locus_tag="PK399_11315"
     CDS             complement(2115..3098)
                     /locus_tag="PK399_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPW13750.1"
                     /translation="MPAHAADEAVATLGGETMGTTWSVRLAAARNTDLHALHAGIQAR
                     LDRVVTQMSTWDATSDISVYNRANAGAWQRIPDKFHRVLTCAVQIAEASDGAFDPTIG
                     SLVGLWGFGADAAIDRAPSSTEREVARGHVGWRRIEIRGDELLQPGGLRLDLSAIAKG
                     FGADCVVDYLRAAGISGALVEVGGELHGIGRKPDGTPWRVLVEAGAEEIDHSLEPRVL
                     ALDGVAVATSGDRWHHYTRRDEHGDERRYTHTIDPRSGAPVEHAPVAVTVIARDAMHA
                     DAWATALTVLGENAGLALAERHGLAVRYLGHDGDGRLRERMTDAFRAHLAQ"
     gene            complement(3104..3976)
                     /locus_tag="PK399_11320"
     CDS             complement(3104..3976)
                     /locus_tag="PK399_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HPW13751.1"
                     /translation="MKRISILAAIIAAAIPLSAIAHKQWLVPSSTVLAGKDAWITVDA
                     AISNALYYPDHNPMRLDNIVITAPDGSTHKPQNPATGKYRSVFDVQLEQQGTYRIANV
                     NGGVSARWDTPESLAAAEAAKDNPAPAAGGMGGTPRGGMLRNATAEDLATKIPKNALN
                     VQVTEGIGRVETFATNGSPSDVAPIGKGLELVPVTHPNDLFAGEAATFRLVVDGKPVA
                     GLEIEIIRGGTRYRNAQEEIKATTDAKGEFSVTWPDAGMYWLETATTDSKTSVPQARQ
                     RRLSYVGTFEVLPQ"
     gene            complement(4003..4533)
                     /locus_tag="PK399_11325"
     CDS             complement(4003..4533)
                     /locus_tag="PK399_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HPW13752.1"
                     /translation="MRVSVSAPVSIAIGTLLVAPAAQAADLSISVEIPRINAAEYHRP
                     YVAIWIEDANQKSVADLQVWYQQTDGKEGHGTKWLPDLRQWWRKSGRSLKVPVDGITA
                     PTRPVGKHALKLDEKHPALAKLPAGTYTLVVEAAREVGGRELLKIPFEWKGKTPSIGK
                     AQGKDELGLVTLSAKP"
     gene            complement(4560..>5121)
                     /locus_tag="PK399_11330"
     CDS             complement(4560..>5121)
                     /locus_tag="PK399_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HPW13753.1"
                     /translation="WISAAVCLVGMLLFSITGITLNHAAQIEGKPEVTTVHDTVPATL
                     LKTLPARGDGKAPLPVGLRGWLRDKHGIVPGDREAEWSEDELYLSMPGPGNDAWLSIQ
                     RADGALEYERTDRGTLAWLNDLHKGRNAGTAWSWFIDVFAVACIVFTATGLFLLYLHG
                     RQRRATWPLVALGLLIPMLLALLFIH"
BASE COUNT          747 a         1685 c         1808 g          881 t
ORIGIN      
        1 acatggagaa cgccggccgc gaacgctacc tgctggtgga cgacgaaggc gcgcgcatcc
       61 cgctgggcga caccgagatc ctggccttgc ccgcgcactt cctgcactcg gtcggcaacc
      121 tgtcgttctt cgatccggtg agtcgcatcc tgttccccgg cgatgtctgt tccgggctgg
      181 tcggcagcgg cgtgccgtac gccttcgtcg aggatttcga ggcgtaccgg ccgtgcatgg
      241 aagggttcca tcgtcgctac atggccagca accgtgcgtt gcggctgtgg gtggacatgg
      301 tccgtgaact cgccccggag atgatcgtgc ctcagcacgg catgcccttc cgcggcgcgg
      361 cgatcaagcc tttgctcgac tggctggaag gcctgcagtg cggcgtggac ctgatgggcc
      421 cggtcaacta tcgaccgaac ccgcagcgcc cgccggtacg cgtgggctac tgaaccgcgc
      481 gtcggttcga gcggcgcatc aatagacgtc gcggcgatag cgcccggcgt cggcaagcgc
      541 cgtcatgcgt gatggcccga ggatctcgtt gagcgccgcg tccacgcccg gggccatgcc
      601 ttccaggctg ccgcagacgt aaacggcggc gccctggtcg acccaagtgc gcaacacgtc
      661 gccattcgcg tgcaatgcat cctgtacgta acgctgcgca tggccgtcgc gagagaacac
      721 caggtcgagc cgctccagta cgcccgcgcg ttgccacgcg tcgagatctt cgccgtgcag
      781 gcggtcggcg ctggcattgc gctcgccgaa cagcagccag ttgcgtgtcg cgcctgcggc
      841 cgcgcgtgcc ttcaggtgcg cgcgcaggcc ggccaggcca gtgccattgc cgatcaggat
      901 catgggctgc gacgccagcg gcgcatggaa attggggttg ctgcggatgc gcaggtcgat
      961 gccgtcgccg atggccgcgt gccggcatag ccagccgctg ccgatgccgg gcgtgccgtc
     1021 ctcgcgctgc atcagcctga ccagcaggtg caggctgccg tcggcgggaa gcgaagcgat
     1081 cgaatattcg cgatgcggca aggcttgcag cgaggcgacc aacgcgcgcg tgtcgttgca
     1141 cacggcatcg gtcatcgccg gcagtcgcga gcgcgacagg atttccgaga gcggagtgtc
     1201 gtcgcgatcg tgcttgacga tgtggcagcc atcgagcgcg tgccttgcga ggaacgccgc
     1261 gacatcgtcc gctgcattgc gcggaccgat ttccgcgatg tcgccggcct gccagtcgag
     1321 cgccatgccg gcgggcggtt gcaactcgat gtgccaggcc tcgccgccga ggctgcccgg
     1381 attgagcagg tggcgcgcgg ccagtgtcca gcggtcgtgg cgcggcgtcg tccagtcggg
     1441 ctggtcggca tgtcccgaga gcacgccgat gcgatgttgc cagtggcgca gcgcggcggg
     1501 gtcggcgttg tcgacgtcga cgcgatcgaa catcggctgc gcgccgtgcc gatgcagcca
     1561 ctcgtcgagt tcgtggccga aggcgcagaa ctgcgcgtat tcgctgtcgc ccaatgcgag
     1621 taccgcatag cgcgtccgcg acaggtcggc aggctgcgtc attacccgct tcacgaagcc
     1681 catcgcgtgg tcgggcgggt cgccttcgcc ggtggtgctg gcgatgaaca ggcaatgcgc
     1741 gccgtcgagc acctgcaggt cgatcgcgtt gatgtcgtgc acgcaggccg gctggccggc
     1801 gtcgcgcagt gcctgcgcgg tgcgttcggc cagatccagg gcgaagccgg tctggctggc
     1861 gtagatcacc cgccacgacg cttccccgtc catgctggcg atgacgcgtg gcgttcgcgc
     1921 gcgccacgcg atcaagccgc agaacgcggc atatgctgcg agtgcggagc ctgcgaacac
     1981 gcgcggttgc gccagtggcg tcactgtcgg ccacggttgc acctgcaagc gcaacagcca
     2041 cccggcgagc aacgcaagcg ccaacccgac cagccactgg ccgatgaggg aacggtcgat
     2101 tcgcgatgcc gtcttcattg cgcgagatgc gcccggaacg cgtcggtcat gcgttcgcgc
     2161 aaccggccgt cgccatcgtg gcccaggtag cgcacggcca gtccgtggcg ttcggccagc
     2221 gccagccctg cgttttcgcc gagcacggtg agcgcggtcg cccacgcatc cgcgtgcatc
     2281 gcatcgcggg cgatcacggt caccgcgacc ggtgcatgtt cgaccggcgc accgctgcgt
     2341 ggatcgatgg tgtgggtgta gcggcgttcg tcgccgtgtt cgtcgcgacg cgtgtagtga
     2401 tgccagcggt cgcctgacgt ggcgacggcg acgccgtcga gtgccagcac gcgcggttcg
     2461 aggctgtgat cgatttcttc agcgccggcc tcgaccagca cccgccacgg cgtgccatcc
     2521 ggcttgcggc cgatgccgtg cagttcaccg ccgacttcga ccagtgcgcc gctgatgccg
     2581 gccgcgcgca ggtagtcgac gacgcaatcc gcgccgaagc ccttggcgat cgcggagaga
     2641 tcgaggcgca ggccgccggg ttgcagcaac tcatcgccgc gaatttcaat gcggcgccag
     2701 ccgacatggc cgcgcgccac ctcgcgttcg gtcgatgacg gcgcacggtc gatggcggca
     2761 tcggcaccga agccccaaag accgacgagc gatccgatcg tcggatcgaa tgcgccgtcg
     2821 ctggcctcgg cgatctgcac cgcgcacgtc agcacgcggt ggaacttatc ggggatgcgc
     2881 tgccacgcac cagcattggc gcggttgtag acgctgatgt cggacgttgc atcccatgtg
     2941 ctcatctgcg tgaccacacg atccagtcgc gcctggatgc cggcatgcag cgcgtgcagg
     3001 tcggtattgc gcgcggcagc gagccgcacg ctccacgtcg tgcccatggt ctcgccgccg
     3061 agcgtggcga cggcctcgtc ggcggcgtga gcaggcaagc ggattactgc ggcaggactt
     3121 cgaacgtgcc gacgtagctc aggcggcgct gcctggcctg cggcacgctg gtcttgctgt
     3181 cggtggtcgc ggtttccagc cagtacatgc ccgcgtccgg ccaggtcacg ctgaactcgc
     3241 ccttggcgtc ggtggtcgcc ttgatctctt cctgtgcgtt gcgatagcgg gtgccgccgc
     3301 ggatgatctc gatctccagg ccagcgaccg gcttgccgtc gaccaccagg cggaacgtcg
     3361 cggcctcgcc cgcgaacagg tcgttgggat gggtgaccgg caccagctcc aggcccttgc
     3421 cgatcggcgc gacgtcgctc gggctgccgt tggtggcgaa ggtctcgacc cggccgatgc
     3481 cttcggtgac ctgcacattc agcgcgttct tcgggatctt cgtcgccaag tcttcggcgg
     3541 tggcattgcg caacatgccg ccgcggggag tgccgcccat accacctgcg gccggcgccg
     3601 gattgtcctt cgcggcctcg gcagcggcca ggctctccgg cgtatcccag cgagcggaca
     3661 cgccgccgtt gacgttggcg atgcggtagg tgccctgctg ctcgagttgg acgtcgaaca
     3721 cgctgcggta cttgccggtc gccgggttct gcggcttgtg ggtgctgccg tccggtgcgg
     3781 tgatgacgat gttgtccagt cgcatcgggt tgtggtcggg gtagtacagg gcattggaga
     3841 tcgcggcgtc gacggtgatc cacgcatcct tgccggcgag cacggtggag gagggcacca
     3901 gccactgctt gtgggcgatg gcggagagcg ggatcgcggc ggcaatgatg gcggcgagga
     3961 tcgagatgcg cttcatgggg tgtgctccgg aacgtgtgcg attcagggct tggcgctgag
     4021 ggtgaccagg ccgagttcgt ccttgccctg ggccttgccg atggacgggg tcttgccttt
     4081 ccactcgaag gggatcttca gcagttcgcg gccgccgact tcgcgtgcgg cctcgaccac
     4141 gagcgtgtag gtgccggccg gcagcttggc gagcgcgggg tgcttctcgt cgagcttgag
     4201 cgcgtgctta ccgaccgggc gcgtgggcgc ggtgatgccg tcgaccggca ccttcaggct
     4261 gcgaccgctc ttgcgccacc actggcgcag gtcgggcagc cacttggtgc catggccttc
     4321 cttgccatcg gtctgctggt accagacctg caggtcggcg accgacttct ggttggcgtc
     4381 ctcgatccag atcgccacgt aggggcggtg gtattcggcc gcgttgatcc gcgggatctc
     4441 gacactgatc gacaggtcgg cggcctgcgc cgcgggcgcg acgagcaggg tgccgatggc
     4501 gatggaaacg ggcgcggaga cagaaactcg catgggttcg cctttggatc gatggagatt
     4561 cagtggatga acagcagtgc cagcagcatc gggatcagca ggcccagtgc gaccagcggc
     4621 caggtggcac ggcgctggcg gccgtgcagg tacagcagga acaggccggt cgcggtgaac
     4681 acgatgcagg ccaccgcgaa cacatcgatg aaccagctcc acgcggtgcc cgcattgcgg
     4741 cccttgtgca ggtcgttgag ccaagccagc gtgccgcggt cggtgcgttc gtattccagc
     4801 gcgccatccg cgcgctggat gctcagccag gcatcgtttc cggggcctgg catcgacagg
     4861 tagagctcgt cctccgacca ttccgcctcg cgatcgccgg gcacgatgcc gtgcttgtcg
     4921 cgcaaccacc cacgcaggcc gaccggcagg ggtgccttgc catcgccccg cgccggcagc
     4981 gtcttcaaca acgtcgccgg cacggtgtcg tggaccgtcg tcacctccgg cttgccctcg
     5041 atctgcgctg catggttgag ggtgatgccg gtgatcgaga acagcagcat gccgaccagg
     5101 cacacggcgg cactgatcca g
//
LOCUS       DAOOCX010000161         5110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_15338_length_5110_cov_7.860930, whole genome shotgun sequence.
ACCESSION   DAOOCX010000161 DAOOCX010000000
VERSION     DAOOCX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15338_length_5110_cov_7.860930"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PK399_11335"
     CDS             complement(<1..763)
                     /locus_tag="PK399_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HPW13754.1"
                     /translation="MQPDTKLPKVGTTIFTVMSQLAAEHGAVNLGQGFPDFPVPQRLV
                     DALGRAMQAGHNQYAPMTGIPALRQAIAGKTERCYGWRPDVDSEVTVTSGATEAIFDA
                     IHAVVRAGDEVIVLDPCYDCYEPAIDLAGAKAVHVALDPLTFAPDWDAVRAAITPKTR
                     MLMVNSPHNPSGAMFGAEDIRALIDLLRGTDIVLLSDEVYEHIVFDGARHESALRHPE
                     LRERAFVISSFGKTYHCTGWKIGYCIAPPALSAEFR"
     gene            867..1514
                     /gene="ccmA"
                     /locus_tag="PK399_11340"
     CDS             867..1514
                     /gene="ccmA"
                     /locus_tag="PK399_11340"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HPW13755.1"
                     /translation="MPDSNVSASPLLAVRGLAFSRNETPVFGPLDFAVDSNEALLVQG
                     GNGAGKTTLLRVLAGLLRADAGHVRIDGQPARHELRARFVAYLGHLPGLKADLSALQN
                     LDFLCGLHGRRKAQTPEAAMAIVGLGGYEDAPLRTLSAGQKKRLGLARLWLSPAPLWL
                     LDEPYANLDLDGITLVNRMVQAHLRDGGAALITTHGAYAAPPVRTRMLELGGAAP"
     gene            1511..2182
                     /gene="ccmB"
                     /locus_tag="PK399_11345"
     CDS             1511..2182
                     /gene="ccmB"
                     /locus_tag="PK399_11345"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HPW13756.1"
                     /translation="MMWQAANALVRRDLRLLWARRGDAFQPALFALLTVVLFALALGG
                     DAESLAPVASAVLWLAVLLAGLLALDSLFRGDAEDGSLEQWMLAPVPLWWLVLARTLM
                     HWATTALPVIVAVPLLGELLHLPRAQLPVLMASLLLGTPILSLLGAVVAALTVGMRRA
                     GVLVALLALPLYVPVLVFGAGSVAASAQGLDAVGALLLLGAGLVLALVLAPLTAAAAL
                     RIAHN"
     gene            2469..2849
                     /gene="azu"
                     /locus_tag="PK399_11350"
                     /pseudo
     CDS             2469..2849
                     /gene="azu"
                     /locus_tag="PK399_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077620.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
     gene            2983..3732
                     /locus_tag="PK399_11355"
     CDS             2983..3732
                     /locus_tag="PK399_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HPW13757.1"
                     /translation="MSPLIRWFHQLGSPPTFDRFAARWTPWAYLLGLLAMGVGLYGAL
                     FQVPADYQQGDSFRILYIHVPSAWMSMAVFGLMACYAAIALIWRIKLCEILAMACAPS
                     GAAFTLVTLATGSIWGKPMWGTWWDWDPRLTTELILLFLYLGVMGLYAAIDDRRNAAR
                     AAGLLAIVGVALLPVIRWSVVWWNSLHQGQTIRMFGESSMDASMIAPLWWMVVGTHLW
                     FVGSLLARARADNLRREAGKDWVGKLAEASR"
     gene            3729..3899
                     /locus_tag="PK399_11360"
     CDS             3729..3899
                     /locus_tag="PK399_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HPW13758.1"
                     /translation="MSYRDYVIAAYAVFAVVMLWDWLAPKLQLRAALRAIRLRAARNA
                     NRAPAANTELER"
     gene            3896..4378
                     /gene="ccmE"
                     /locus_tag="PK399_11365"
     CDS             3896..4378
                     /gene="ccmE"
                     /locus_tag="PK399_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HPW13759.1"
                     /translation="MNPTRKRRLWLVLAVVAAAALATTLIALALQRNVAYLYTPNEVL
                     TGEAGANVTSGEARFRLGGMVAADSLKRTPGTLDARFEVDDGDARLPVVYTGILPDLF
                     REKQAVVATGRMQGGVFVAEEVLAKHDEAYMPKEVADKMGMAHKKHDVPDAQAPASRP
                     "
     gene            4378..>5110
                     /gene="ccsA"
                     /locus_tag="PK399_11370"
     CDS             4378..>5110
                     /gene="ccsA"
                     /locus_tag="PK399_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPW13760.1"
                     /translation="MGFLPELGQVALILALLAACMQALLPMLGAQRGVAALMAVGRPA
                     AYLQLALVGLAFAILTHGFIAQDFSQRYVAENSNSLLPMIYRYTAVWGAHEGSLLLWA
                     LILALWTAAVARFSRALPMPVVARVLGVLGVVAVGFLAFLIFTSNPFERLLPAAAEGR
                     DLNPLLQDPGMIIHPPMLYLGYVGFSVPFAFAIAALLEGRVDAQWLRWTRPWTNVAWA
                     FLTVGIALGSWWAYYELGWGGWWFWD"
BASE COUNT          678 a         1783 c         1794 g          855 t
ORIGIN      
        1 tgcggaactc cgcgctcaag gccggcggcg cgatgcagta gccgatcttc cagccagtgc
       61 agtggtaggt cttgccgaag ctggagatga cgaaggcgcg ctcacgcagt tccggatggc
      121 gcagcgcgga ttcgtggcgg gcgccatcga acacgatgtg ctcgtagacc tcgtcgctga
      181 gcagcacgat gtcggtgccg cgcagcaggt cgatcaacgc ccggatgtct tcggcgccga
      241 acatcgcccc ggacgggttg tgcgggctgt tgaccatcag catgcgggtc ttcggcgtga
      301 tcgccgcgcg caccgcgtcc cagtcgggcg cgaaggtgag cgggtccagc gcgacgtgaa
      361 ctgccttcgc cccggcgagg tcgatggccg gctcgtaaca gtcgtagcag gggtcgagga
      421 cgatcacctc gtcgccggcc cgaacgacgg catggatcgc atcgaagatc gcctcggtgg
      481 cgccgctggt gaccgtgacc tcgctgtcga catcgggtcg ccagccatag cagcgttcgg
      541 tcttgccggc gatcgcctgg cgcaaggccg ggatgccggt catcggcgcg tactggttgt
      601 ggccggcctg catcgcccgg cccagcgcat cgaccaggcg ttgcggcacc gggaaatccg
      661 ggaagccctg ccccaggttg accgcgccgt gctcggcggc cagctgcgac atcacggtga
      721 agatggtggt gccgaccttc ggcagtttgg tgtctggctg catcgcgatg tggggatggc
      781 gtgggagccc gagtttacgg aatcgcgccg gccgcacgcg tgaccgatcc catgccgatg
      841 gtgcatgccg ctaaaatcgg tcgcccatgc ccgattccaa cgtctccgcc agtcccctgc
      901 tcgccgtgcg cgggctggcg ttctcccgca acgagacgcc ggtgttcggg ccgctggatt
      961 tcgcggtgga ttccaacgag gccttgctgg tccagggcgg caacggggcc ggcaagacca
     1021 ccctgctgcg cgtgctcgcg ggcctgctgc gggccgatgc cggccatgtg cggatcgacg
     1081 gtcaaccggc gcggcatgaa ttgcgcgcac gcttcgtggc ctatcttggc cacctgccgg
     1141 gattgaaggc ggatctctcg gcgctgcaga acctggactt cctgtgcggc ctgcatggtc
     1201 ggcgcaaggc gcagacaccg gaagcggcga tggccatcgt cggcctgggc ggctacgagg
     1261 atgcgccctt gcgcacgctc tccgccggcc agaagaagcg gctcgggctg gcccggctgt
     1321 ggctgtcgcc ggcgccgctg tggttgctcg acgagcccta cgccaacctc gacctcgacg
     1381 gcatcaccct ggtcaaccgg atggtgcagg cgcatctgcg cgatggcggg gcggcgttga
     1441 tcaccaccca cggcgcgtat gccgcaccgc ccgtgcgcac gcgcatgctg gaactcggcg
     1501 gtgccgcgcc atgatgtggc aagccgcaaa tgcgctggtc cggcgcgacc tgcgattgct
     1561 gtgggcgcgt cgcggcgacg cgttccagcc ggcgttgttc gcactgttga ccgtggtcct
     1621 gtttgccctg gcgctgggtg gcgatgccga atcgctggca ccggtcgcgt ccgccgtgct
     1681 ctggctggcg gtgctgctgg ccggcctgct cgccctggac agcctgttcc gcggcgacgc
     1741 cgaggacggc tcgctcgagc aatggatgct ggcaccggta ccgctgtggt ggctggtgct
     1801 ggcgcgcacg ctgatgcact gggcgaccac ggcgctccct gtcatcgtcg ccgtgccgct
     1861 gctcggcgaa ctgctgcacc tgccgcgcgc gcaactgccg gtgctgatgg ccagcctgct
     1921 gctgggcacg ccgatcctga gcctgctcgg cgcggtggtg gccgcgctga cggtgggcat
     1981 gcgccgcgcc ggcgtgctgg tcgcgctgct ggccttgccg ctgtatgtcc cggtgctggt
     2041 gttcggtgcc gggtcggtcg cggcaagcgc acagggactc gatgccgtcg gcgcgctgct
     2101 gctgctgggc gcgggcctgg tgctggcgct ggtcctggca ccgctgacgg cggccgcagc
     2161 gctgcgcatc gcccacaatt gaccggggtc aatgtgcgcg agcgcgccgc gcgcgacgat
     2221 ggcggcgatc ccggtccgac gggccggcct tacgcgctcc aggagctacc cccatgaagt
     2281 cccgccacgt cctcgccgcc agcgcgctcg cgcttgccct ctccgcctgc ggccagtcgc
     2341 ccgcgccaac cgccgagccc ccggttgccg caccggcaac ggaagaggct gcagcccccg
     2401 cgccggttga agccgccccg gtcgcgcccg cgcccgtcgc cgccaccggc agcaagcccg
     2461 ccaccgtcgc cgattgcgcc accacgatcg agggcaacga cgccatgcag tacaacgcgg
     2521 actcgatcac catccccgcc agctgcaccc agttcaccat caccctcaag cacgccggtg
     2581 caatggcggt gaacgtgatg ggccacaacg tggtggtggc caaggaagcc gacatggccg
     2641 ggatcgccgc cgacggcatg tcgctgacgc cggaacacgt gaagcccgac gacgcccgca
     2701 tcatcgccca caccaggatg gtgggtggcg gcgaatccgc gtcggtgacc ttcgacgtgg
     2761 ccaaggtcaa ggacggcggc ccgttcaagt tcttctgcag tttccccggc cacctggcgc
     2821 tgatgcaggg caacctggtg gtgcaatgag tgcatcgcac ggcggctcgg gtgggtggta
     2881 ccaggccacc cgcgccgccg tgcgaaggca gtcccgcacg aggggctggt atccttcccg
     2941 gattgccgcg gccgcatgtg cgccgcaccc gcacaggccg cgatgtcccc gctgatccgc
     3001 tggttccacc aactcggttc cccgcccacc ttcgatcgct tcgccgcccg ttggacgccg
     3061 tgggcgtacc tgctcgggtt gctcgcgatg ggcgtcggcc tgtacggcgc gctgttccag
     3121 gtgccggccg attaccagca gggcgacagc ttccggatcc tctacatcca cgtgcccagc
     3181 gcgtggatga gcatggcggt gttcgggttg atggcgtgct atgcggccat cgcgctcatc
     3241 tggcggatca agctgtgcga gatcctggcg atggcctgcg cgcccagcgg cgcggccttc
     3301 accctcgtca ccctggccac cggctcgatc tggggcaagc cgatgtgggg tacctggtgg
     3361 gactgggacc cgcgcctgac caccgaactg atcctgctgt tcctctacct gggcgtgatg
     3421 ggcctgtacg ccgccatcga cgaccgccgc aacgcggcgc gcgccgccgg cctgctggcg
     3481 atcgtcggcg tggccctgct gccggtgatc cgctggtcgg tggtgtggtg gaactcgctg
     3541 caccagggcc agaccatccg catgttcggt gagtcctcga tggacgccag catgatcgcg
     3601 ccactgtggt ggatggtggt cggcacccat ctctggttcg tcggctcgct gctggcgcgc
     3661 gcgcgcgccg acaacctgcg ccgcgaggcc ggcaaggact gggtcggcaa gctcgcggag
     3721 gcttcgcgat gagttaccgc gactacgtca tcgccgccta tgcggtgttc gcggtggtga
     3781 tgctgtggga ttggctcgcg ccgaaactgc agttgcgcgc cgccctccgg gcgatccgcc
     3841 tgcgcgctgc ccgcaacgcc aaccgcgcgc ccgccgcgaa cacggaactc gaacgatgaa
     3901 cccgacccgc aagcgccgcc tgtggctggt gctggccgtg gtggccgccg ccgcgctggc
     3961 caccaccctg atcgcgctgg ccctgcagcg caacgtggcc tacctgtaca cgccgaacga
     4021 agtcctgacc ggcgaagccg gcgcgaacgt cacctccggc gaagcccgct tccgcctggg
     4081 cggaatggtc gcggcggatt cgctcaagcg cacccccggc accctggacg cgcgcttcga
     4141 ggtggatgac ggcgacgcac gcctgccggt ggtctacacc ggcatcctgc ccgacctgtt
     4201 ccgcgagaag caggcggtcg tcgccaccgg tcgcatgcag ggcggcgtgt tcgtcgccga
     4261 ggaagtgctg gccaagcacg acgaggccta catgccgaag gaagtcgccg acaagatggg
     4321 catggcgcac aagaagcacg acgtgcccga cgcgcaagcc ccggcgtcgc gcccctgatg
     4381 gggttcctcc ctgaactcgg ccaggtcgcc ctgatcctgg cgctgctggc cgcctgcatg
     4441 caggcgctgt tgccgatgct gggcgcgcaa cgcggcgtgg cggcgctcat ggcggtcggc
     4501 cggcccgcgg cctacctgca actggcgctg gtcgggctgg cgttcgccat cctcacccac
     4561 ggtttcatcg cccaggattt ctcgcagcgc tacgtcgccg agaactccaa ctcgctgctg
     4621 ccgatgatct accgctacac cgcggtgtgg ggcgcgcacg aaggctcgct gctgctgtgg
     4681 gcgctgatcc tggcgctgtg gaccgcggcg gtggcacgct tctcgcgggc gttgccgatg
     4741 ccggtggtcg cgcgcgtgct cggcgtgctc ggcgtggtcg cggtcgggtt cctcgccttc
     4801 ctgatcttca ccagcaatcc gttcgaacgc ctgttgccgg ccgcggcgga aggccgcgac
     4861 ctcaacccgc tgctgcagga cccggggatg atcatccatc cgccgatgct gtacctgggc
     4921 tacgtcgggt tctcggtgcc gttcgcgttc gcgatcgccg ccctgctgga aggtcgcgtc
     4981 gatgcgcagt ggctgcgctg gacgcggccg tggaccaatg tcgcctgggc gttcctcacc
     5041 gtcggcatcg cgctgggcag ctggtgggcg tactacgaac tcggctgggg cggctggtgg
     5101 ttctgggatc
//
LOCUS       DAOOCX010000162         5096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_15396_length_5096_cov_6.432057, whole genome shotgun sequence.
ACCESSION   DAOOCX010000162 DAOOCX010000000
VERSION     DAOOCX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15396_length_5096_cov_6.432057"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PK399_11375"
     CDS             <1..236
                     /locus_tag="PK399_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13761.1"
                     /translation="QYQPTGMWFTIAGNRYSARSGGGTIDASADVVGFRGGAYDGWRG
                     ARRGDAIVFRKDDHANPRAGEGIKVGDFRCGRR"
     gene            complement(265..2679)
                     /locus_tag="PK399_11380"
     CDS             complement(265..2679)
                     /locus_tag="PK399_11380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPW13762.1"
                     /translation="MTDPVERQRRAMAAFDALVDLDDDTRERRIREVCGDDADLLEEV
                     RAMLAADAREGQPFRGDAAAWGQALHEPPASREGEPMPGRQLGAWRIVEAIGRGGMGT
                     VYAVQRSDGAYAQRAALKLIRTAADSPAARERFLRERQLLAQLRHPNIATLLDGGFSD
                     DGDPYFVMEYVDGAPIDQWCDTRTLGLRGRVQLFVQVLEAVGHAHRNLVIHRDLKPSN
                     LLVDGEGRVKLLDFGIAKQLEGSEATATSDRALTFEYASPEQLHAAPITTATDIWQLG
                     IVLHRLLSGSHPFGLARDTPLAKQLQLLEREPEPLTRAATQASVQQAALRGEADPSAL
                     AKSLRGGLAAIVETCLRRSPEARYASADALAADLQRWLEHRPVDAAQLGKGERVALWL
                     RRNRLLAAAVGAVAIALIAGTSIALWQANEARSQARIAGQQRSEAQLQAKRARQAFVF
                     LQDAMFGVAPQGQLDAQFSLNDLLARSRETLEQDPQFDPQARKAMQRTLANLYAIGQD
                     WGTAETLYRQGLQDPAAPYDRDDATELAAQFGNYAQVLGVMGKPKEAIAAAQRAAALR
                     RQYLPDDVFEQAVSLRNLGDAYLGDGDNDHARRNYEQAIAAFRTVDIADQVQAIDALV
                     ASIGLARALNAQAQYAAALRTVDEATAFADRQGLAADWDGRAVLLSLKATAQKNLGDA
                     GGALASFREATEIAGRIYGVDSPALANGHIGIGGALNDLGRPREALDAYARAERIIAG
                     KAVQPGTDAKILAGQGMAWGKLGEEGKRTDYYRRALAALEKADSAPLLELKRRISAEA
                     SAAPPR"
     gene            complement(2676..3233)
                     /locus_tag="PK399_11385"
     CDS             complement(2676..3233)
                     /locus_tag="PK399_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HPW13763.1"
                     /translation="MEPAPPIEVTRLLARARAGDAQAMGDVFSALYDELKRAARLQLR
                     RMHDDFETTALVHEAYLKLGGAQLAAQDRNHLLALSARAMRQVLVDDARARKADKRGG
                     GQDALTLGALANAGEAAAVEVLKLDELLDALHRLDERAAQVVELRYFGGYTEPEVAGM
                     LGISESTLRRDWRRARAFLMKETAP"
     gene            3385..4857
                     /locus_tag="PK399_11390"
     CDS             3385..4857
                     /locus_tag="PK399_11390"
                     /inference="COORDINATES: protein motif:HMM:NF015831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HPW13764.1"
                     /translation="MNKQANHGRRAPALSLLSLALLGALSMPAFAAEPVCLDTDGNPS
                     GETTTQGQEHGSQNTTCDAASAAYGIENNASAFFSAAFGLRNTANGNWSSAFGRDNTA
                     SGYASSAFGSNNGASGNSSNAFGFDNRAIGFNANAFGGANTAGADYSSAVGYRNTASG
                     AASSAFGYRNTAIGERSSAFGYASQALNIGSTAIGYMAIADRDYAVAVGRAGGERQII
                     HLADGTEDFDAVNVRQLRSLTNWFGGGATFTGGMFTAPTFTIQGTNYNTIAAAFAAVD
                     SRLTALGDGVGVPGPQGEPGPAGPQGEQGPQGEQGPAGQDADLGQVAQMVDAGDAATL
                     AAANTHADAGDAATLAAAKAHATAGDAATLTASKSYTDSTATQTLNSAKSYTDAKFAV
                     WNDTFIQYQQQVDKRFAQTDRRIDQIGAMGTAMTQMAVNAANGNSAKGRIAIGVGAQG
                     SQGAVSIGYGKRIGDRGSFSLGASFSSGESSVGGGFGFDL"
BASE COUNT          761 a         1746 c         1753 g          836 t
ORIGIN      
        1 tgcagtacca gcccaccggg atgtggttca ccatcgccgg caatcgttat tccgctcgat
       61 caggtggcgg cacgatcgat gcctccgcgg atgtcgtcgg tttccgcggc ggcgcctacg
      121 acggctggcg cggcgcgcgc cgcggtgatg ccatcgtgtt tcgcaaggac gaccacgcca
      181 atccgcgggc tggtgagggc atcaaggtcg gcgatttccg ctgcggcagg cggtagggct
      241 gaagtttcgc gatgaccagc gcggtcagcg cggcggcgcg gccgatgcct cggcgctgat
      301 ccgtcgctta agctccagca acggtgcgct gtcggccttc tcaagggcgg ctagcgcgcg
      361 gcggtagtag tccgtgcgct tgccttcttc gcccagcttg ccccaggcca tgccttggcc
      421 ggccaggatc ttcgcatcgg tgccgggctg caccgccttt ccggcgatga tgcgctccgc
      481 tcgggcatag gcgtcaagcg cttcgcgtgg gcggccgagg tcgttcagtg cgccgccgat
      541 gccgatatgg ccattggcca gcgccgggct atccacgccg tagatgcgac ccgcgatctc
      601 ggtcgcttcc cggaagctgg ccagggcgcc gccggcatcg ccaagatttt tctgtgcggt
      661 ggccttcagg ctcagcaaca ccgcgcgccc atcccagtct gctgccagtc cctgcctgtc
      721 ggcgaaggcc gtggcttcgt cgacggttcg caatgccgct gcatactgcg cctgcgcgtt
      781 cagcgcacgg gccaggccga tcgaggccac cagcgcatcg atcgcctgca cctggtcggc
      841 gatgtcgaca gtcctgaacg ccgcgatcgc ctgttcgtag ttcctgcgcg catggtcgtt
      901 gtcgccatcg ccgaggtagg cgtcgccgag attgcgcagc gagaccgcct gttcgaatac
      961 gtcgtcgggc aggtattgcc ggcgcagggc cgccgcgcgc tgggcggccg cgatcgcttc
     1021 cttcggcttg cccatcacgc ccagcacctg cgcgtagttg ccgaactgcg ccgccagctc
     1081 cgtcgcgtcg tcgcggtcgt agggcgctgc gggatcctgc aagccctgcc gatacagcgt
     1141 ttcggcagtg ccccagtcct ggccgatggc atacaggttg gccagggtcc gctgcatcgc
     1201 cttgcgtgcc tgcgggtcga attgcggatc ctgctccagt gtctcccgcg accgtgccag
     1261 caggtcgttc aggctgaact gtgcgtcgag ttggccctgc ggcgccacgc caaacatcgc
     1321 atcctgcagg aagacgaagg cttggcgcgc ccgcttggcc tgcagttgcg cttcgctgcg
     1381 ctgctgcccg gcgatgcgcg cctgagaccg cgcctcgttg gcctgccaaa gcgcaatgct
     1441 ggtgccggca atcaacgcaa tcgcgaccgc accgaccgcg gcggccagca ggcgattgcg
     1501 tcgcaaccac agcgcgacgc gttcgccctt gccgagctgc gccgcgtcga caggccggtg
     1561 ctccagccag cgttgcaggt cagcggccag cgcgtccgcg ctggcgtagc gtgcctcggg
     1621 gctgcgccgc aggcaggtct cgacgatcgc ggccagtccg ccgcgcagcg acttcgccaa
     1681 ggccgatgga tccgcttcgc cacgcagtgc ggcctgttgc acgctggcct gcgtggcggc
     1741 gcgggtcagc ggttccggtt cgcgttccag cagctgcaac tgttttgcca gcggggtgtc
     1801 gcgggccagg ccgaatgggt gcgagccgct cagcagacga tgcagcacga tgcccagctg
     1861 ccagatgtcg gtggcggtgg tgatcggcgc ggcatgcagt tgttcgggcg aggcgtattc
     1921 gaaggtcagc gcgcggtcgc tggtggcggt ggcctcgctg ccttcgagct gcttggcgat
     1981 gccgaagtcg agcagcttca cccggccttc gccatcgacc agcaggttcg agggtttcag
     2041 gtcgcgatgg atcaccaggt tgcggtgcgc atggccgacc gcctccagca cctgcacgaa
     2101 caactgcacg cgcccgcgca atcccagcgt gcgcgtgtcg caccactggt cgatgggtgc
     2161 gccgtccacg tactccatga cgaagtacgg gtcgccgtcg tcgctgaagc cgccgtcgag
     2221 cagggtggcg atgttcgggt ggcgcagctg cgccagcagc tggcgttcgc gcaggaagcg
     2281 ttcgcgcgcg gctggcgaat ccgccgcggt gcggatcagt ttcaacgcgg cgcgttgcgc
     2341 gtaggcgcca tcgctgcgtt gcaccgcgta gaccgtgccc atgccgccgc ggccgatggc
     2401 ctcgacgatg cgccaggcgc ccagctggcg ccccggcatc ggctcgccct cgcggctcgc
     2461 cgggggttcg tgcaaggcct gtccccaggc cgcggcatcg ccacggaagg gctggccctc
     2521 gcgcgcatcc gccgccagca tcgcgcgcac ctcttcaagc aggtcggcat cgtcgccgca
     2581 gacctcgcgg atccgtcgtt cgcgcgtgtc gtcatcgagg tcgaccagcg catcgaacgc
     2641 cgccatcgcg cgacgctggc gttcgaccgg atccgtcacg gcgcggtttc cttcatcagg
     2701 aaggcgcgtg cgcgccgcca gtcgcgacgc agggtgctct cgctgatgcc gagcatgccg
     2761 gcgacttccg gttcggtata gccgccgaag taacgcagtt ccacgacctg cgcggcgcgc
     2821 tcgtcgagcc ggtgcaaggc atccagcagc tcgtcgagct tgagtacctc gaccgccgcg
     2881 gcttcgccgg cattggcgag cgcgccgagg gtgagcgcgt cctgcccgcc gccgcgcttg
     2941 tcggccttgc gcgcgcgtgc atcgtctacc agcacctggc gcatcgcccg cgcggagagc
     3001 gccagcaggt ggttgcggtc ctgtgccgcc agttgtgcgc cgccgagctt caggtaggcc
     3061 tcgtggacca gcgcggtggt ctcgaaatcg tcgtgcatgc gccgcaactg caggcgcgcg
     3121 gcacgcttga gttcgtcgta caacgccgag aacacgtcgc ccatcgcctg cgcgtcgcct
     3181 gcgcgcgcgc gcgccagcag gcgggtcact tcgatcgggg gcgcgggctc caccgtgttt
     3241 cgctcggcat gcattgaaac tggcggaagt gtcgcacagg cgccgcgcgc ccggactgga
     3301 aggcggcgtg aacggatccg ccgccgcaat ccgcataccc atccaagacc ggcattccgc
     3361 cgttgtcatc cgaggtggag acgcatgaac aagcaagcca atcacggtcg gcgcgcgccg
     3421 gccctgtccc tgctgagcct ggccttgctg ggggcgttgt cgatgccggc gttcgcggcc
     3481 gagccggtat gcctggacac ggacggcaat ccgtccggtg aaaccacgac gcaagggcag
     3541 gagcacggca gccagaacac cacctgcgac gcggcgagtg ccgcctacgg catcgagaac
     3601 aatgccagtg ccttcttcag cgccgcgttc ggcctgcgca acaccgccaa cggcaactgg
     3661 agttccgcgt tcggtcgcga caacaccgcc agtgggtacg ccagcagcgc cttcggctcg
     3721 aacaacgggg cgagcggcaa ttcgagcaat gcattcggtt tcgacaaccg tgccatcggt
     3781 ttcaacgcca acgcgttcgg tggcgcgaac acggccggtg ccgactacag cagtgccgtg
     3841 ggttaccgga acacggccag cggcgcggcg agcagtgcct tcggctatcg gaataccgcc
     3901 atcggcgagc gcagcagtgc gttcggctac gcatcacagg cactgaacat cggcagcacc
     3961 gcgatcggct acatggcgat cgcggatcgc gattacgccg tcgccgtggg cagggctggc
     4021 ggcgaacgcc agatcatcca tctggcagat ggcaccgagg atttcgatgc ggtgaacgtg
     4081 cgtcagctgc gttcgctcac gaattggttc ggcggtggcg caacctttac cggcggcatg
     4141 ttcaccgcgc cgaccttcac cattcagggc acgaactaca acaccattgc ggcggccttt
     4201 gcggcggtgg attcgcggct gaccgcattg ggcgatggcg tcggcgtgcc cggcccgcag
     4261 ggcgagcctg gtccggcagg tccgcaaggc gagcaaggtc cgcagggcga gcagggcccc
     4321 gccggacagg atgcggacct gggccaggtt gcgcagatgg tcgatgccgg cgatgccgcc
     4381 acgcttgcag cagccaacac gcatgccgac gcgggcgatg ccgccacgct cgccgcggcc
     4441 aaggcgcatg ccactgcggg cgatgcggcc acgctgactg catcgaagag ctacaccgac
     4501 agcaccgcca cgcagacgtt gaactcggcc aagtcttata ccgatgcgaa gttcgcggtg
     4561 tggaacgaca ccttcatcca gtaccagcag caggtggaca agcgctttgc gcagaccgac
     4621 cgccgcatcg accagatcgg cgcgatgggc acggcgatga cccagatggc agtgaacgcg
     4681 gcgaacggca attccgccaa gggccgcatc gcgatcggcg tgggcgcgca gggcagccag
     4741 ggcgcggtgt cgatcggcta cggcaagcgc atcggcgacc gcggttcgtt ctcgctgggc
     4801 gcgagcttca gcagcggcga atcgtcggtg ggtggcggtt tcggtttcga cctgtaacgc
     4861 actcgttccc ggaagacgaa acggcgcggg caaccgtgcc gtttccgttg gcggcatcgg
     4921 gcgtcagcgc agctcgtcca cgcgggcatt cgcgcaatgc gcgaagttca ccggcacgcc
     4981 gtgcgcgccc aacaccgcgg cgaattgccg ttggcgtgcc tggaattgcg cgaagccgtg
     5041 ctccagccgc gcgcaatcgg attcgcccgc ggcattgcgt gcccgccacg ccgtgg
//
LOCUS       DAOOCX010000163         4979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_15897_length_4979_cov_9.299959, whole genome shotgun sequence.
ACCESSION   DAOOCX010000163 DAOOCX010000000
VERSION     DAOOCX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15897_length_4979_cov_9.299959"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /gene="actP"
                     /locus_tag="PK399_11395"
     CDS             complement(<1..352)
                     /gene="actP"
                     /locus_tag="PK399_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304713.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HPW13765.1"
                     /translation="MKRMHSFLFALAMLLFAGAAWAGGGDLGQAEKQPINWTAISMFA
                     VFVCATLWITKRAAAKTRSAADFYTAGGGITGFQNGLAIAGDYMSAASFLGISAAVMA
                     NGYDGLIYSIGFLVG"
     gene            complement(349..666)
                     /locus_tag="PK399_11400"
     CDS             complement(349..666)
                     /locus_tag="PK399_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HPW13766.1"
                     /translation="MSDMIDPMVARIEANPKYHELKRKRSSFGWLLTVLMLLVYYGYI
                     GLIAFDKELLARPLGAGVTTLGIPIGLGVIAFTVIITGLYVRRANDEYDRLTAEILED
                     ATP"
     gene            complement(699..2075)
                     /locus_tag="PK399_11405"
     CDS             complement(699..2075)
                     /locus_tag="PK399_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HPW13767.1"
                     /translation="MHTPTTTGRRAPLAIALFVALLAPGLAFAQSAKEQELEARIAQL
                     EAQVQALIGAQQQQQATIVDTRAQLDQVKVAQAAPADGKPHIQSTPILTAGNPDGRFS
                     YGGFIKVDAMMTDTNAGEIADGSAGRMFYLPSAIPVGGADESHVDTDTHAQFSRFWFA
                     ADGDMQGHKTRAYLEFDLFGGGSNQLGNEAATNTHALTLRQAYVTWDKWLAGQTWSNF
                     QDVAALPDAVDFVGPTEGTTFVRQAQLRYTSGPFSVALENPQTVLTGYRSTTRINSDD
                     GGMPDITARWTQKGDWGHVSIAGLARQLRHDTAATSASGSGFGVSVSGKYNIGTHDDI
                     RYMLTGGQGIGRYVGFALGADGALDATGADIDGTGVLAGFVAWRHVFDPKLRGNLMVS
                     RAQFDNDTDWTGFGVTESAQSIHANLIYSPFPKLDIGAELIWGNREVESGADGDLRRL
                     HTSVKYSF"
     gene            2250..4202
                     /gene="acs"
                     /locus_tag="PK399_11410"
     CDS             2250..4202
                     /gene="acs"
                     /locus_tag="PK399_11410"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HPW13768.1"
                     /translation="MTGQQERYHVPAAFAAHSNMRHRDYVREYQAAIDDPDAFWGRIA
                     QRIDWMQAPTRIKDVSFNADDFHIRWFDDGELNASVNCLDRHLAEHGDKVAIIFEPDS
                     PDAPSQRITYRELHARTCKLANALRNLGVEKGDRVTIYLPMIPEAAVAMLACARIGAV
                     HSVVFGGFAPQSIADRVQDCGTKLVITADEGLRGGRKVPLKANVDEALKLPGTNSVET
                     VLVVRHTGGAVDMQMPRDRWYDAVVDGQPDTCEPERMNAEDPLFILYTSGSTGKPKGV
                     LHTTAGYLVWAAYTHEIVFDLKPDDIYWCTADVGWITGHSYIVYGPLANCGTSVMFEG
                     IPSWPNASRFWEVIDKHQVTIFYTAPTAIRALMREGDHAVKRTSRKSLRLLGSVGEPI
                     NPEAWRWYHDVVGEERCPIVDTWWQTETGGILISPLPGAIDLKPGSATLPLPGVQVAL
                     VDAAGTILEGETSGNLVLLDSWPGQMRTVYGDHQRFIDTYFKAYPGMYFTSDGCRRDA
                     DGYYWITGRVDDVINVSGHRIGTAEVESALVSHHDVAEAAVVGYPHDIKGQGIYAYVT
                     LKQGIEPTDELRKALVQQVRKEIGPTASPDHIQWAPSLPKTRSGKIMRRILRKIAENA
                     PEQLGDISTLADPSVVESLVNERQVR"
     gene            4252..4911
                     /locus_tag="PK399_11415"
     CDS             4252..4911
                     /locus_tag="PK399_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW13769.1"
                     /translation="MTTLLIADDHPLFREALRGVALRLLPGARIREAENAEALYAMAD
                     DEPDADLLLLDLNMPGTQGFSALVHLRAAHPQLPVAMVSAREEPEIMRRALDHGAMGF
                     IPKSSDAATLATALQQVLDGERWVPDAALKAAPIADDERAIAARVRELTPQQFRVLQM
                     LGTGLLNKQIGYELGVSEATIKAHMSAILRKLGANNRTQAVLLAGKLAVDPKRVVAQS
                     E"
BASE COUNT          844 a         1699 c         1644 g          792 t
ORIGIN      
        1 agccaaccag gaagccgatc gagtagatca ggccgtcgta gccgttggcc atcactgctg
       61 cggaaatgcc caggaacgag gccgccgaca tgtagtcgcc ggcgatcgcc aagccgttct
      121 ggaagccggt gatgccgccg ccggcggtgt agaagtccgc cgccgaacgc gtcttcgccg
      181 cggcgcgctt ggtgatccac aacgtggcgc agacgaatac cgcgaacatg ctgatcgcag
      241 tccagttgat cggttgtttc tcggcctggc cgaggtcgcc accgccggcc cacgcggcac
      301 ctgcgaacag cagcatcgcc agcgcgaaga ggaacgaatg catgcgcttc atggggtcgc
      361 gtcctccagg atctcggcgg tcaggcggtc gtactcgtcg ttggcgcggc gcacgtacag
      421 gccggtgatg atcacggtga acgcgatcac gccgagcccg atcgggatgc cgagcgtggt
      481 cacgcccgca cccagcggcc tggccagcaa ttccttgtcg aaggcgatca ggccgatgta
      541 gccgtaatag accagtagca tcagcacggt cagcagccag ccgaagctgg agcgcttgcg
      601 cttgagttcg tggtacttcg ggttggcctc gatccgggcc accatggggt cgatcatgtc
      661 ggacatgcgg agtctccgtt gcgtgtcggg tggcgggatc agaagctgta cttgacgctg
      721 gtatgcaggc ggcgcaggtc gccgtcggcg ccgctctcca cctcgcggtt gccccagatc
      781 agttccgcgc cgatgtccag cttcgggaac ggactgtaga tcaggttggc gtggatcgat
      841 tgcgcggact cggtgacgcc gaagccggtc cagtcggtat cgttgtcgaa ctgcgcgcgc
      901 gagaccatca ggttgccgcg cagcttggga tcgaacacgt gccgccaggc cacgaagccg
      961 gccagcacgc cggtgccgtc gatgtcggcg ccggtggcat ccagcgcacc gtccgcgccc
     1021 agcgcgaagc ccacgtaacg gccgatgccc tggccgccgg tgagcatgta gcggatgtcg
     1081 tcgtgcgtgc cgatgttgta tttgcctgac acgctcaccc cgaaaccgct gccgctggcg
     1141 ctggtggccg cggtgtcgtg gcgcaactgg cgggccaggc cggcgatgct gacgtggccc
     1201 caatcgcctt tctgcgtcca gcgcgcggtg atgtccggca tgccgccatc gtcgctgttg
     1261 atgcgggtgg tgctgcgata gccggtgagc acggtctgcg ggttttccag cgccaccgag
     1321 aagggcccgc tggtgtagcg cagctgcgcc tggcggacga aggtggtgcc ctcggtcggg
     1381 ccgacgaaat ccaccgcgtc cggcagcgcc gccacgtcct ggaagttcga ccaggtctgt
     1441 ccggccagcc acttgtccca ggtcacgtag gcctggcgca gggtcagcgc atgggtattg
     1501 gtcgcggcct cgttgcccaa ctggttgctg ccgccgccga acaggtcgaa ctccaggtag
     1561 gcgcgggtct tgtggccctg catgtcgccg tcggcggcga accagaagcg cgagaactgc
     1621 gcatgcgtgt cggtgtcgac gtgcgattcg tccgcgccac ccaccgggat cgcgctgggc
     1681 aggtagaaca tgcgccccgc gctgccgtcg gcgatctcgc cggcgttggt gtcggtcatc
     1741 atcgcatcga ccttgatgaa gccgccgtag ctgaagcggc catccggatt gccggcggtg
     1801 aggatcggcg tggactggat gtgcggcttg ccgtcggcgg gcgcggcctg cgcgaccttg
     1861 acctggtcga gctgcgccct ggtgtcgacg atcgtggcct gctgttgctg ctgtgcgccg
     1921 atcagtgcct gcacctgcgc ttccagctgc gcgatgcgcg cttcgagttc ctgctctttc
     1981 gcggactgcg cgaatgcgag ccccggcgcc agcagtgcga cgaacagggc gatcgccaat
     2041 ggcgcgcggc gaccggtggt cgtgggtgtg tgcatggttc cccctcccaa gggacgcacg
     2101 accatgtgcc gtgcagggcg accatgcgct ggcaggcttg cgggcgtcga ttcgcctttg
     2161 gtcgaagatg gcgccatttg cgaccaaggt ctaacgccgc cgccgcgcga ttgcggcaca
     2221 ctcgctgcat cgtcctcggg agggatgcca tgaccggcca gcaagagcgt taccacgtcc
     2281 ccgcagcatt cgccgcgcat tcgaacatgc gccaccgcga ctacgtccgc gaataccagg
     2341 cggcgatcga cgaccccgac gcgttctggg gccgcatcgc ccagcgcatc gactggatgc
     2401 aggcgccgac gcggatcaag gacgtcagct tcaacgccga cgacttccac atccgctggt
     2461 tcgacgatgg cgaactcaat gccagcgtca attgcctgga ccgccacctc gccgagcatg
     2521 gcgacaaggt cgcgatcatc ttcgagccgg acagtcccga cgcaccgtcg cagcgcatca
     2581 cctatcgcga gctgcatgcg cgcacctgca agctcgccaa cgcgctgcgc aacctcggcg
     2641 tcgagaaggg cgaccgcgtc accatctacc tgccgatgat ccccgaggcc gccgtcgcga
     2701 tgctcgcctg tgcgcgcatc ggcgcggtgc actcggtggt gttcggcggc ttcgccccgc
     2761 agtcgatcgc cgaccgcgtg caggactgcg gcaccaagct ggtgatcacc gccgacgaag
     2821 gcctgcgcgg cggccgcaag gtgccgttga aggcgaacgt ggatgaagcg ctgaagctgc
     2881 ccggcaccaa ttcggtggaa accgtactgg tggtccgcca caccggcggc gcggtggaca
     2941 tgcagatgcc gcgcgaccgc tggtacgacg ccgtggtcga tggccagccc gacacctgcg
     3001 aaccggagcg catgaacgcg gaagatccgc tgttcatcct ctacacctcc ggcagcaccg
     3061 gcaagccgaa gggcgtgctg cacaccaccg ccggttatct cgtctgggcc gcctacaccc
     3121 acgagatcgt gttcgacctc aagccggacg acatctactg gtgcaccgcc gacgtgggct
     3181 ggatcaccgg tcacagctac atcgtctacg gaccgctggc caactgcggc accagcgtga
     3241 tgttcgaagg catccccagc tggccgaacg cctcgcgctt ctgggaagtg atcgacaagc
     3301 accaggtcac gatcttctac accgcgccga ccgcgatccg cgcgctgatg cgcgaaggcg
     3361 accatgcggt gaagcgcacc tcgcgcaagt cgctgcggct gctgggcagc gtcggcgaac
     3421 cgatcaaccc cgaagcctgg cgctggtacc acgacgtggt cggcgaggaa cgttgcccga
     3481 tcgtggacac gtggtggcag accgagaccg gcggcatcct gatctcgccg ctgcccggtg
     3541 ccatcgacct caagccgggt tccgccaccc tgccgctgcc gggcgtgcag gtcgccctgg
     3601 tcgatgccgc cggcaccatc ctggaaggcg agacctccgg caacctggtg ctgctggatt
     3661 cgtggccggg gcagatgcgc acggtgtacg gcgaccacca gcgcttcatc gacacctact
     3721 tcaaggccta cccgggcatg tacttcacca gcgatggttg ccggcgcgat gccgacggct
     3781 attactggat caccgggcgc gtggacgacg tgatcaacgt cagcggccac cgcatcggca
     3841 cggcagaagt ggagagcgcg ctggtctcgc accacgacgt cgccgaagcc gcggtcgtcg
     3901 gctacccgca cgacatcaag ggccagggca tctacgccta cgtcaccctg aagcagggca
     3961 tcgagcccac cgacgagctg cgcaaggcgc tggtccagca ggtgcgcaag gaaatcggac
     4021 cgaccgccag ccccgaccat atccagtggg cgcccagcct gccgaagacc cgcagcggca
     4081 agatcatgcg gcgcatcctg cgcaagatcg cggagaacgc acccgagcag ctcggcgaca
     4141 tcagcaccct ggccgatccg tcggtggtgg aatcgctggt caacgaacgc caggtgcgat
     4201 gatcacgggg aagcggcacc gcccgatgcc gcttgttcct tgctggatgc catgaccacc
     4261 ctcctcatcg ccgacgacca cccactgttc cgcgaagccc tgcgtggcgt ggccctgcgc
     4321 ctgctgcccg gcgcacgcat ccgcgaggcg gaaaacgcgg aagcgctgta cgccatggcc
     4381 gatgacgagc ccgatgcgga cctgctgctg ctggacctca acatgcccgg cacgcagggc
     4441 ttctccgcac tggtgcacct gcgcgccgcg catccgcaac tgccggtggc gatggtttcc
     4501 gcgcgcgaag aacccgagat catgcggcgc gcactggacc acggcgcgat gggcttcatc
     4561 cccaaatcct ccgacgccgc gacgctggcc accgcgttgc aacaggtcct cgacggcgaa
     4621 cgctgggtgc ccgacgccgc actcaaggcc gcgcccatcg ccgacgacga acgcgccatc
     4681 gccgcgcgcg tgcgcgaact caccccgcaa caattccgcg tgctgcagat gctcggcacc
     4741 ggcctgctca acaagcagat cggctacgaa ctcggcgtgt cggaagccac catcaaggcg
     4801 cacatgagcg ccatcctgcg caagctcggc gccaacaacc gcacccaggc cgtgttgctc
     4861 gccggcaagc tggcggtgga tccgaagagg gtggtggcgc aaagcgagtg atggctgcta
     4921 tcggccgagg ctgtgtgaaa actcccccgt cgctggcaag attcaagcac cagtcgatt
//
LOCUS       DAOOCX010000164         4933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_16095_length_4933_cov_7.469045, whole genome shotgun sequence.
ACCESSION   DAOOCX010000164 DAOOCX010000000
VERSION     DAOOCX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16095_length_4933_cov_7.469045"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1894)
                     /locus_tag="PK399_11420"
     CDS             complement(<1..1894)
                     /locus_tag="PK399_11420"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPW13770.1"
                     /translation="MGVFGIGGRGKDDGEEKGKLKLDPAQAAKALPLLVALLALLGIW
                     CLASGALRIRDDARHAGLQVARDDAVMAARRTLNDERGRLAQRLGADRVKAALATGDS
                     AATLRLLRQGWPELQRAELWPADLTAAYAGLPRTGFGALATCEAALAEGKPVARVVKS
                     AGVQRLALAAPVVGGSGPMVAYVELPLEKLASGVEAPTVPSDTYLALRQGSRNVLERG
                     DTSLGNSAEALAIKVPDSDLRIAASTPDATATPFELGGMPAVGLGAILVLLSAWLWVI
                     VRPRLLGLRGNDGADAEPTLADLQRDQPAPEPAVREKVAEDTKADAVPVAIDRGIFRA
                     YDIRGVMGQTLDASVAELIGQAVGSLMFDQGLDEIVVGRDGRLSGPELAEGLIAGLRK
                     AGRNVIDLGMVPTPVVYFGCYHLRTGCGVSITGSHNPPDYNGFKIVVGGETLSGNAIL
                     DLHARIAEDRLLETKTPGTLSERDIGEDYVHRIAGDVQVGQRLRVVVDAGNGVAGALG
                     PRVLEAIGADVTPLFCDIDGTFPNHHPDPSEPHNLEALVQMVQRLDADLGIAFDGDGD
                     RLGVVTKSGKYIFPDRLLMLFAADVLERNPGAVIVYDVKCTGRLPTHILRHGGSPLMW
                     KTGHSLI"
     gene            complement(1907..2365)
                     /gene="dut"
                     /locus_tag="PK399_11425"
     CDS             complement(1907..2365)
                     /gene="dut"
                     /locus_tag="PK399_11425"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPW13771.1"
                     /translation="MAQALQVKLLDPRFGGEWPLPAYATEHSAGLDLRAAIDAPLLLA
                     PGETALVPSGLAIHIGDPGLCAVILPRSGLGHKHGIVLGNGTGLIDADYQGPLMISTW
                     NRGDAGYTISPGDRIAQLVLLPIVRATLQVVDTFEESARGEGGFGHTGVR"
     gene            complement(2365..3605)
                     /gene="coaBC"
                     /locus_tag="PK399_11430"
                     /pseudo
     CDS             complement(2365..3605)
                     /gene="coaBC"
                     /locus_tag="PK399_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007964144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
     gene            3703..4377
                     /gene="radC"
                     /locus_tag="PK399_11435"
     CDS             3703..4377
                     /gene="radC"
                     /locus_tag="PK399_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPW13772.1"
                     /translation="MHIRDWPEGERPREKLLAHGAGSLSDAELLAIFLGSGTAGRDAV
                     ASARDLLAAEGGLRALLDREPRAMTRLRGIGPARACALSAALELGHRHLAAQLERGEM
                     LGDPAAAGRYFAQRLRGLAHEVFAALFLDTRHRALGFEELFRGSIDGAEVHPRALVER
                     ALARGAAAVIIGHNHPSGNPEPSAADRAVTARIKQALGLVDIRLLDHFVIGDGPPLSM
                     AGRGLV"
     gene            4421..4660
                     /locus_tag="PK399_11440"
     CDS             4421..4660
                     /locus_tag="PK399_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13773.1"
                     /translation="MGNAENLMLEHLKRFQAGQDRIERKLEDLTRRAGNLEAGQAAII
                     QHLGALAVTDAQQQVLLDGYNARLERIERRLELAG"
     gene            4722..>4933
                     /gene="argS"
                     /locus_tag="PK399_11445"
     CDS             4722..>4933
                     /gene="argS"
                     /locus_tag="PK399_11445"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300787.1"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPW13774.1"
                     /translation="MKTQLRALIEQGLSALRAAGTLPVDLASPDFSVERPKERAHGDF
                     STNAAMLLARELGKSGAKTNPRALAQ"
BASE COUNT          749 a         1791 c         1658 g          735 t
ORIGIN      
        1 tgatcagcga atgcccggtc ttccacatca gcgggctgcc gccgtggcgg aggatatggg
       61 tgggcaaacg accggtgcat ttcacgtcgt acacgatcac cgcgcccggg ttgcgctcca
      121 gcacatcggc ggcgaagagc atcagcaggc ggtccgggaa gatgtacttg cccgacttgg
      181 tgaccacacc caggcggtcg ccgtcgccat cgaaggcgat gccgaggtcg gcatcgaggc
      241 gctggaccat ctgcaccagt gcttccaggt tgtgcggctc gctcgggtcc ggatggtggt
      301 tggggaaggt gccgtcgatg tcgcagaaca gcggggtgac gtcggcaccg atcgcctcca
      361 gcacgcgcgg gcccagcgcg ccggccacgc cgttgccggc atcgaccacc actctcaggc
      421 gctggccgac ctggacgtcg ccggcgatcc ggtgcacgta gtcctcgccg atgtcgcgct
      481 cgctgagcgt gccgggcgtc ttggtttcga gcaggcggtc ttcggcaatg cgtgcatgca
      541 ggtcgaggat ggcattgccc gacagggttt ccccgccgac cacgatcttg aagccgttgt
      601 agtcgggcgg gttgtggctg ccggtgatcg acacgccgca gccggtgcgc aggtggtagc
      661 agccgaagta caccaccggc gtcggcacca tgcccaggtc gatcacgttg cgaccggcct
      721 tgcgcaggcc cgcgatcagg ccctcggcca gttccgggcc ggacaggcgg ccatcgcggc
      781 cgaccacgat ctcgtcgagc ccctggtcga acatcagcga accgacggcc tggccgatca
      841 gttcggccac gctggcatcc agggtctggc ccatcacgcc gcggatgtcg taggcacgga
      901 agatcccgcg gtcgatcgcc acaggcaccg catcggcctt ggtgtcttcg gccaccttct
      961 cgcgcaccgc cggttcggga gccggttggt cgcgctggag gtcggccagg gtcggctcgg
     1021 catccgcgcc gtcattgccg cgcaagccca gcaggcgcgg acgcacgatg acccacagcc
     1081 atgcggacaa cagcaccagg atggcgccca ggccaaccgc tggcatgccg ccaagctcga
     1141 acggcgtggc agtggcgtcg ggcgtcgatg cggcgatgcg caggtcgctg tccggcacct
     1201 tgatggccag cgcctcggcg gaattgccca ggctggtatc accgcgctcc agcacgtttc
     1261 ggctgccctg gcgcagggcc aagtaggtgt cggaggggac agtcggtgcc tcgacgccgc
     1321 tggccagctt ctccagcggc agctcgacgt aggccaccat cggaccgctt ccaccgacca
     1381 ccggcgccgc cagggcaagg cgctggaccc cggcagattt caccacccgc gctaccggct
     1441 tgccttcggc cagcgcggct tcgcaggtgg ccagtgcgcc gaacccggtg cgcggcaggc
     1501 cggcgtacgc ggcggtcagg tcggccggcc acaattcggc acgttgcagt tcgggccagc
     1561 cctggcggag caggcgcagg gtcgcggcac tgtcgccggt cgccagcgcg gccttgaccc
     1621 ggtcggcacc cagtcgctgc gcgagccgcc cgcgttcgtc gttgagggtg cgccgcgccg
     1681 ccatcaccgc atcgtcgcgg gccacctgca ggccggcatg gcgtgcatca tcgcggatgc
     1741 gcagggcacc gctggccagg caccagatgc cgagcagggc cagcagtgcg accagcagcg
     1801 gcagcgcctt cgccgcctgc gccggatcca gcttcagctt gcccttttct tcgccgtcgt
     1861 ccttgccacg accgccgatt ccgaacacac ccatctcgat cccccgtcaa cgcacgccgg
     1921 tatggccgaa tccgccttcg ccgcgcgcgc tttcctcgaa agtatccacc acctgcaacg
     1981 tcgcgcgcac gatcggcagc aggaccagct gggcgatgcg gtcgccggga gagatcgtgt
     2041 agcccgcatc gccacggttc caggtgctga tcatcagcgg cccctggtag tcggcatcga
     2101 tcaggccagt gccgttgccc agcacgatgc catgcttgtg gcccaacccg gagcgcggca
     2161 ggatcaccgc gcacagaccg gggtcgccga tatggatggc gagcccggac ggcaccaatg
     2221 cggtttcgcc cggggccagc agcaatggcg cgtcgatcgc ggcgcggagg tccagcccgg
     2281 cgctgtgctc ggtcgcgtag gccggcagcg gccactcccc gccgaaacgc ggatccagca
     2341 gtttcacctg cagggcctgc gccatcagcc tgcgccctgc atcgacaggc aaccggccac
     2401 gatatccagc aactcgccgg ccaacatggt cttcgaggcc ggacccaggg cgcgttcgcc
     2461 gtcctggtcg atcaccagca gcgcgttgtc gtcgctctcg aagccgccgc cggccacgcc
     2521 gacccggttc gcacagatca ggtccacgcc ctttcgctcc cgcttgccgc gggcatagcc
     2581 ggccacgttg tcggtctcgg cggcgaaccc caccaccagc cgagggcgcg ccgcgtgcgc
     2641 ggccacgtcc gccaggatgt cgcgggtctt gaccagttcc agcaccaggg tgtcctggcc
     2701 cggctgcttc ttcagcttgt tcgccgatgc cgcgcgcggg gtgaaatccg ccaccgccgc
     2761 ggcgccgatg taggcatcgc acggggcgtg ccccgcctcc aggcgcgcca tcaccgtcgc
     2821 atgcatctgc gccgcgctgc gcacgtcgat gcgggtgacg ccgggcggcg tggccatgtg
     2881 cacgggcccg gccaccagca cgacctcggc accgcgttcg gccgccaccg cggcgatcgc
     2941 gaagcccatc ttgccgctgc tgcgattgcc caggaaccgc accggatcga tgtcttcata
     3001 ggtcggcccg gcgctgacca gcaatcgcac ccccgccagc gacgcactca tcgcgcgact
     3061 cccagcgcgg ccacgatggc cgcgggttcg gccatccgac cagggccgga ttcgccctcg
     3121 gctagggggc cgtcgtcggg tccgatcacc tgcacgccgc gcgaacgcag cacggcgaca
     3181 ttcgcctggg tcgcaggatg ccgccacatc cggtggttca tcgccggggc gattgccagc
     3241 ggcgcctcgg tggccaggca gagggtgctg accaggtcgt cggccagccc atgcgccagc
     3301 ttggcaatgg tgttggcggt cgccggcgcc acgatcactc gttgcgccca gccggccagt
     3361 tccaggtggc ccatcgcggc ctcggcggtt tcgtcccaca agctcgtgcg taccgggcga
     3421 tgcgacaagg cctggaaggt ctgcgcggtc aagaaccgct gcgcgttgtc ggtcatggcg
     3481 acctgcacat cggcaccggc atcgcgcaac cggcgcagca gttctgcggc cttgtaggcg
     3541 gcgatcccgc cgcagacgca gagcaggacg cggtgcgtat cgggggttgt aggcggagcc
     3601 tgcatccggc cggtttcctg accacgtgac gggcgaccag cttacccgat ggtcgatgcc
     3661 gcaccgatgc cgcatcgcga cgcaccgatg cacgctggcc gcatgcacat ccgtgactgg
     3721 ccagaaggcg aacgcccccg cgaaaaactg ctggcccacg gcgccggcag cctgtccgac
     3781 gccgaactgc tggcgatctt cctgggctca ggcacggccg gccgcgatgc ggtggccagc
     3841 gcgcgcgacc tgctcgccgc ggaaggcggc ctgcgcgccc tgctcgatcg cgagccacga
     3901 gcgatgaccc ggctgcgcgg gatcggcccg gcccgcgcct gcgcattgtc cgcggcactg
     3961 gaactgggcc accgccacct ggccgcacaa ctcgagcgcg gcgaaatgtt gggcgatcct
     4021 gctgcggccg ggcgctattt cgcccagcgc ctgcgcggcc tggcccacga ggtgttcgcc
     4081 gcgctgttcc tcgacacccg ccatcgcgca ctgggcttcg aggaattgtt ccgcggcagc
     4141 atcgacggcg ccgaagtgca cccgcgcgcg ctggtcgaac gcgcgctggc gcgcggcgcg
     4201 gcggcggtca tcatcggcca caaccacccc agcgggaatc ccgagcccag cgccgccgac
     4261 cgcgcggtca ccgcgcgcat caagcaggcg ctggggctgg tcgacatccg cctgctcgac
     4321 cacttcgtga tcggcgacgg tccgccgctg tcgatggcgg ggcgggggct ggtatgaggc
     4381 ctcacccgct accaacctgc taccctcaat cgaggtttcc atgggcaatg ccgagaacct
     4441 gatgctcgag catctaaaac gcttccaagc cgggcaggat cgcatcgagc gcaagcttga
     4501 agatttgacg cgccgcgccg gcaaccttga agccgggcaa gcggcgatca tccagcacct
     4561 tggcgcactt gccgtcactg atgcccagca gcaagtgctg ctcgacggct acaacgcacg
     4621 gctggaacgc attgaacggc ggctggagtt ggcgggctga aacgtcgcgc gagccgcctc
     4681 gcgtacaatg cgcagccatt gaaacggctg cctgatcctt cgtgaaaacc cagctccgcg
     4741 ccctgatcga acagggcctg tccgccctgc gtgccgccgg caccctgccc gtcgaccttg
     4801 cctcgcccga cttctccgtc gagcgaccga aggagcgcgc ccacggcgac ttctcgacca
     4861 atgcagcgat gctgctggcc cgtgagcttg gcaagagcgg ggcgaagacc aatccgcgcg
     4921 cgctggcgca gca
//
LOCUS       DAOOCX010000165         4931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_16105_length_4931_cov_9.323216, whole genome shotgun sequence.
ACCESSION   DAOOCX010000165 DAOOCX010000000
VERSION     DAOOCX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16105_length_4931_cov_9.323216"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PK399_11450"
     CDS             <1..633
                     /locus_tag="PK399_11450"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HPW13775.1"
                     /translation="GLDRPMPSATADGITMINFGMGSPNAATMMDLLSAIEPKAVLFL
                     GKCGGLKKKNQLGDLVLPIAAIRGEGTSNDYMPPQVPALPAFALQRAVSTMIRDLELD
                     YWTGTVYTTNRRVWEHDDEFKEKLRAMRCMAIDMETATVFAAGFANRIPCGALLLVSD
                     QPMIPEGVKTEASDAKVSAEYVQNHIKVGIEALRLIRRHGKSVRHLRFDE"
     gene            745..1281
                     /locus_tag="PK399_11455"
     CDS             745..1281
                     /locus_tag="PK399_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HPW13776.1"
                     /translation="MIVPGELLAFWRDAGMGKWFRGGDDFDAECRARWLDAHFAAARR
                     AFDDWMDAADSALALVLLLDQIPRNVFRKSGHAFATDPLALHYAGRAIDAGFDAQVDP
                     SLRFFFYLPFEHSEAIADQDRAVDLFTALGNADLLGYANAHRDVIVRFGRFPHRNAPL
                     GRNNTADEQAWLEAGGGF"
     gene            complement(1385..>1794)
                     /locus_tag="PK399_11460"
     CDS             complement(1385..>1794)
                     /locus_tag="PK399_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13777.1"
                     /translation="ADPAQAAPAQSAPSATGPGAIGTPPEGKGLVVFFREKKFAGSAI
                     RYKVREGTTELGTLLSGGYFVHVAEPGAHAYTVHSEAKDVMNLEVEAGETYFVIGGIT
                     MGFMAGRPNLSPSDQAVFDSMKAKLKLTAPLKH"
     assembly_gap    1795..1894
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1950..2387)
                     /locus_tag="PK399_11465"
     CDS             complement(1950..2387)
                     /locus_tag="PK399_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13778.1"
                     /translation="MVDIEIGEPISSKRHKRGDKFALRLAEPLVVDGQTVLPAGTPGV
                     GEIIHAAASGGGGKAGELLLAARYLEVDGRQIPLRGFRMGGAGKDNSPGALAASIAVG
                     PFAQFIHGREIEIPSGARASAKLAADTALIPIDTPSIPTGNNP"
     gene            complement(2597..3523)
                     /gene="grxD"
                     /locus_tag="PK399_11470"
     CDS             complement(2597..3523)
                     /gene="grxD"
                     /locus_tag="PK399_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005419209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HPW13779.1"
                     /translation="MSLDPAVRARIESLLATNPVVLFMKGNPNAPQCGFSSKAVGALD
                     AAGADYAHVDVLSDPEIREGIKAYGDWPTIPQLYIRGELVGGSDIILQMAGSGELHAA
                     LGLPAPDRTPPAITITPAAADLLRKAIDDAGDGYALQIDIDKGFNARLQLAPRDEAAI
                     ASESAGIRAQFDLASAARARGLVIDWADDERGKGLVIDNPNAPPKVRNLTPAQASEQA
                     RAGTLTIVDVRPADERALASINVAYATFDDGPGEIEGWPKDTPLAFLCHHGGRSAQAA
                     EHFRALGFSKVHNITGGIDAWAAHVDSSVGTY"
     gene            complement(3609..4238)
                     /locus_tag="PK399_11475"
     CDS             complement(3609..4238)
                     /locus_tag="PK399_11475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPW13780.1"
                     /translation="MSRLAYLALGDSYTIGEGVDEAGRWPVQLASALRASGLAIDDPR
                     IIATTGWTTDELSVAMDAQEPLGEWAFVSLLIGVNNQYRGRAVDDYLGEFTRLLQRAI
                     ALAGDRAGRVLVLSIPDWGVTPFAFASGRDAQAIADDLDAYNAAARDACEAHGVAFVD
                     ITAISRDHGDATVMLADDGLHPSAAQYARWADAALPVAIGLLGDKKGSE"
     gene            complement(4235..>4931)
                     /locus_tag="PK399_11480"
     CDS             complement(4235..>4931)
                     /locus_tag="PK399_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW13781.1"
                     /translation="NYPRALLERIVAIAQKHRLLLLVDEIYDGILYDDNTFQPVAPLA
                     GDLPCMTFSGLSKVHRACGWRVGWAVLSGDPMACGDFHHAMDLLGALRLCANVPGQFA
                     IEQALHGTDTITPLCQPGGRLYETRRALIESCAASPHLSLVAPAGALYGFPAVVGDAA
                     RGFDDHAFALEMLETEDVLLVPGSSFNVPYRNHFRVTLLPEASVLREVFSRIDRVLER
                     RAVTATGQRSAVA"
BASE COUNT          731 a         1609 c         1710 g          781 t
ORIGIN      
        1 ggcctggacc ggccgatgcc cagcgccacc gccgacggca tcaccatgat caatttcggc
       61 atgggcagcc cgaacgcggc gacgatgatg gacctgctgt ccgcgatcga acccaaggcg
      121 gtgctgttcc tcggcaagtg cggcggcctg aagaagaaga accagctcgg cgacctggtg
      181 ctgccgatcg cggcgatccg cggcgagggc accagcaacg attacatgcc gccgcaggta
      241 ccggccctgc cggcattcgc gctgcagcgc gcggtgtcga cgatgatccg cgacctcgaa
      301 ctcgactact ggaccggcac cgtctacacc accaaccgcc gggtctggga acacgacgac
      361 gagttcaagg aaaagctgcg cgcgatgcgc tgcatggcga tcgacatgga aaccgccacc
      421 gtgttcgccg ccggcttcgc caaccgcatc ccctgcggtg cgctgctgct ggtcagcgac
      481 cagccgatga tcccggaagg cgtcaagacc gaagccagcg acgccaaggt ctccgccgag
      541 tacgtgcaga accacatcaa ggtcggcatc gaggcactgc ggctgatccg tcgccacggc
      601 aagtcggtgc ggcatttgag gttcgacgag tgagcgagga cgcgtcttgc gcggcactgc
      661 cgcgcagtcc tcgcgaacgc acggccatgg atggccgggc caggggcatc gcaagtcgct
      721 attgttgcgc catggagggc tgcgatgatt gttcccggtg aactgctcgc gttctggcgc
      781 gacgccggca tgggcaagtg gttccgcggc ggcgacgact tcgacgccga atgccgtgcg
      841 cgctggctgg acgcgcattt cgccgccgcg cgccgcgcgt tcgacgactg gatggacgcc
      901 gccgacagcg cgctcgcgct ggtcctgctg ctcgaccaga tcccgcgcaa cgtgttccgc
      961 aagagcggtc atgcgttcgc caccgatccg ctggccctgc attacgcggg tcgtgccatc
     1021 gatgccggtt tcgatgcgca ggtcgatccg tcgctgcggt tcttcttcta cctgccgttc
     1081 gagcattccg aggcgatcgc cgaccaggat cgcgcggtgg acctgttcac ggccctcggc
     1141 aatgcggacc tgctgggcta cgcgaatgcg catcgcgacg tgatcgtgcg cttcggccgc
     1201 ttcccgcatc gcaatgcgcc gctggggcgc aacaacacgg ccgacgaaca ggcatggctg
     1261 gaggcgggcg gcgggttctg agcggcgcgc gcgggcagac ctccggttcg agggaggccg
     1321 gccgcgcatg aaaaaaggcg gaacctcgcg gttccgcctt tttgtcatcg aaacacgcgt
     1381 gcgctcagtg cttcagcggc gcggtcagct tgagcttggc cttcatgctg tcgaacaccg
     1441 cctggtcgga cggcgacagg ttcgggcgcc ccgccatgaa gcccatggtg atgccgccga
     1501 tcacgaagta ggtctcgcct gcctcgactt ccaggttcat gacgtccttg gcctcggaat
     1561 ggacggtgta ggcgtgtgca cccggctccg cgacgtgcac gaaatagccg ccgctgagca
     1621 gcgtgcccag ttcggtcgtg ccttcacgca ccttgtagcg gatggcggag ccggcgaatt
     1681 tcttctcgcg gaagaacacc accaggccct tgccttcggg cggcgtgccg atcgcgccag
     1741 gaccggtcgc actcggcgcg gactgggcgg gggcggcttg cgccgggtcg gcggnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacggcg gtcggggcca gcagtgcgag
     1921 gcagaggatc agcgacttca gggtttgcgt cacgggttgt ttcctgtggg tatggatggg
     1981 gtgtcgatcg ggatcaacgc ggtatcggct gcgagcttcg cgctggcgcg ggcgccggac
     2041 ggtatctcga tctcgcggcc atggatgaac tgtgcaaaag ggccgactgc gatgctggcg
     2101 gccagggcgc ccgggctatt gtccttgccg gcaccgccca tgcggaatcc gcgcagcggg
     2161 atctgccggc cgtcgacctc caggtatcgc gcggccaaca acaattcgcc tgcctttcca
     2221 ccgccgccgg aagccgctgc gtggatgatc tcgccgaccc cgggggttcc cgccggcaat
     2281 accgtctggc catccaccac cagcggttcg gccagccgca atgcgaattt gtcgccgcgc
     2341 ttgtggcgct tcgagctgat cggctcaccg atctcgatgt cgaccatcgc gccattgacc
     2401 gccaggcagc agccagtgcc ggccgggttg gcgtcgacag ccgattgtcc cgggggcacg
     2461 gcctggtcag ggcttgcggc ggcgggcggg ttcgcgggtg gatcctggcg ggcggctgca
     2521 gtgccgatct gcagcaatgc cgccaacggc cacgcccatt tcatcgaagc gctgcgcgcc
     2581 accgcatcgc gcgccatcaa taggttccga cgctgctgtc gacgtgcgcg gcccaggcgt
     2641 cgatgccgcc ggtgatgttg tgcaccttgc tgaaacccag cgcgcggaaa tgttccgccg
     2701 cctgcgcgct gcggccgccg tggtggcaga ggaaggccag cggcgtgtcc ttcggccagc
     2761 cttcgatctc gcccgggccg tcgtcgaagg tcgcgtaggc gacgttgatg gacgccagtg
     2821 cgcgctcgtc ggccggacgc acgtcgacga tggtcagcgt gccggcccgt gcctgttcgc
     2881 tggcctgcgc gggggtgagg ttgcgcacct tgggcggcgc gttcgggttg tcgatcacca
     2941 ggcccttgcc gcgttcgtcg tcggcccagt cgatcaccag gccgcgcgcg cgcgcggcgc
     3001 tggccaggtc gaactgcgcg cggatgccgg cagactcgct ggcgatggcg gcctcgtcgc
     3061 gtggcgccag ctgcaggcgc gcgttgaagc ccttgtcgat gtcgatctgc agcgcgtagc
     3121 catcgccggc atcgtcgatt gccttgcgca gcaggtccgc cgcggcgggg gtgatggtga
     3181 tcgccggcgg cgtgcgatcc ggcgccggca ggccgagcgc ggcatgcagt tcgccgctgc
     3241 cggccatctg caaaatgatg tccgagccgc cgaccagttc gccgcggatg tacagctgcg
     3301 ggatcgtcgg ccagtcgccg taggccttga tgccctcgcg gatctcggga tcggacagca
     3361 cgtccacgtg cgcgtagtcg gcgccggctg cgtccagcgc gcccaccgcc ttcgacgaga
     3421 acccgcactg cggcgcgttc gggttgccct tcatgaacag cacgacggga ttggtggcca
     3481 gcagcgattc gatgcgggcg cgaacggcgg ggtcgaggga catggggggt tccgggaagc
     3541 gaaaaggggt cagagtgaat tttgcgccga ttgtgtccgg cttgcgcgaa gacggtggtc
     3601 gcaaaacttc actctgaccc ctttttgtcg ccaagcaacc cgatggccac cggcaacgct
     3661 gcatccgccc agcgcgcgta ctgcgccgcc gacgggtgca ggccgtcatc cgccagcatg
     3721 acggtcgcat cgccgtgatc gcggctgatc gcggtgatgt cgacgaaggc cacgccatgc
     3781 gcctcgcaag catcgcgcgc ggcggcgttg taggcatcca ggtcgtcggc gatggcctgc
     3841 gcatcgcggc cgctggcgaa ggcgaacggg gtcacgcccc agtccgggat cgacagcacc
     3901 agcacgcggc cggcgcgatc cccggccagc gcgatcgcgc gctgcagcag gcgggtgaat
     3961 tcgcccaggt agtcatccac cgcgcgtccg cggtactggt tattgacgcc gatcagcagc
     4021 gagacgaacg cccactcgcc cagcggttcc tgcgcatcca tcgccaccga tagttcgtcg
     4081 gtggtccagc cggtggtggc gatgatgcgc gggtcgtcga tggcgaggcc ggatgcgcgc
     4141 aaggcgctgg cgagttgcac gggccagcgg ccggcctcgt cgacgccttc gccgatggtg
     4201 tagctgtcgc cgagggcgag gtaggccagt cggctcacgc caccgcgctg cgttgcccgg
     4261 tcgcggtcac cgcacgccgt tccagcacgc gatcgatgcg cgagaacact tcgcgcagca
     4321 ccgacgcttc cggcagcagg gtgacgcgga agtggttgcg gtagggcacg ttgaagctgg
     4381 agccgggcac cagcagcacg tcttcggtct ccagcatctc cagcgcgaac gcgtggtcgt
     4441 cgaagccgcg cgcggcatcg cccacgaccg ccgggaagcc gtagagcgca ccggccgggg
     4501 cgaccagcga cagatgcggg ctggccgcgc agctttcgat cagcgcgcgg cgggtttcgt
     4561 acaggcggcc accgggctgg cacagcgggg tgatggtgtc ggtgccgtgc agggcttgct
     4621 cgatcgcgaa ctggcccggc acgttggcgc acaggcgcag tgcgccgagc aggtccatcg
     4681 cgtggtggaa gtcgccgcag gccatcgggt cgccgctcag caccgcccag cccacccgcc
     4741 agccgcaggc gcggtggacc ttggacagcc cgctgaaggt catgcagggc aggtcgccgg
     4801 ccagcggcgc gaccggctgg aacgtgttgt cgtcgtagag gatgccgtcg tagatctcgt
     4861 cgaccagcag cagcaggcga tgcttctggg cgatcgcgac gatccgctcc agcaacgcgc
     4921 gcggatagtt g
//
LOCUS       DAOOCX010000166         4911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_16205_length_4911_cov_9.948105, whole genome shotgun sequence.
ACCESSION   DAOOCX010000166 DAOOCX010000000
VERSION     DAOOCX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16205_length_4911_cov_9.948105"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PK399_11485"
     CDS             <1..372
                     /locus_tag="PK399_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPW13782.1"
                     /translation="STSRMMDPNVIGNEHYETARRVQGTLQKYKELKDIIAILGMDEL
                     SEEDKQAVARARKIERFFSQPFTVAEVFTGSKGKYVPLKDTIRGFKGIVDGEYDHLPE
                     QAFYMVGSIDEAVEKAKKLAA"
     gene            417..842
                     /locus_tag="PK399_11490"
     CDS             417..842
                     /locus_tag="PK399_11490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPW13783.1"
                     /translation="MASTIRCDIVSAEAEIFHGEAELVVATGELGELGIAPKHAPLIT
                     RLKPGKVVVTLPGGEKLDFAIGGGILEVQPTVVTVLADTAVRAQDIDEAAVRAAKEEA
                     ERIIAHRGEKMDIAEAQQKLAEVTAQLQALERLRKNLKH"
     gene            complement(992..1381)
                     /locus_tag="PK399_11495"
     CDS             complement(992..1381)
                     /locus_tag="PK399_11495"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPW13784.1"
                     /translation="MTLRRHLGGYAVIGLLQWLVEYGLMLALSEWVMPVEPANVIGRL
                     CGAMLGFWLNGKWTFAADDTHVGRHAAIRFIVMWLALTVLNTWIVAHLDDAFGLRMAQ
                     LLKPGADLLSGGIGFLLSRHWVYRKRT"
     gene            complement(1378..3360)
                     /locus_tag="PK399_11500"
     CDS             complement(1378..3360)
                     /locus_tag="PK399_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13785.1"
                     /translation="MPERTHPSPIPALLSWTGIALCVVGLVAHRMWQELPFPRAFEHG
                     LLALLALAAAWPLQRWRQWSRAAALGAAWLLALAVFSGPMPVLATALLAAAAIGVGSL
                     VLRGPIALPIGLAMIAGTLGWLLSLPIHHRAVYALACIAVVAWRRRAILDALRGAWHT
                     FDAGVRASPAAATGALLLLGLASTAAWLPTMQSDDVAYHLGLPSQLQATARYAMDARL
                     QVWALAPWNGDVLQGVAQVLAGGEARGAMNALWLIVAAGAMHGLAGALGGDATRRWWA
                     VALMASLPLTMHLAAGMQTELVAMAWLPALAWLVLRDDGTSSPRSVLAGALLFGALCG
                     LKPMHGVVALPMLAWAAWRHRAHLPWRALPVGALLAAICGGASYVHAWLLTGNPVLPL
                     FNSLFRSPFFAPRDFSDPRWNGGFDADLVWDITFDTGHYFEAYPGGFGFVLVAMLGAW
                     LLALHDRRTRGLAVVASMGLLLTLLPLQYARYLQPSLVLLLPALVVAWPPLRGGAVTF
                     WGVCVLNLAFATNANWMLRTGALKRDIGHPLDDAPLLLRYAPERLLARRLPADGTLVL
                     VLPGNYGALAELGQRGRNVSWYSPAWETEFARVEADPSGRAWASLLQRERIGHVILDD
                     RKASAALHAGLAIAKARKTDEAADSQRWSLPMETAR"
     gene            3460..4833
                     /gene="glmU"
                     /locus_tag="PK399_11505"
     CDS             3460..4833
                     /gene="glmU"
                     /locus_tag="PK399_11505"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPW13786.1"
                     /translation="MSAPLHVVVLAAGEGKRMKSALPKVLQKVAGRPMLAHAIDAARA
                     LSPQGIHVVYGHGGEAVRAAFADQPDLHWAQQVQQLGTGHAVQQAMPGVPDDVRVLVL
                     YGDVPLITAHSLRRLMDAPGRLAVLAAELPDPTGYGRILRDPEGRVASIIEQKDADEE
                     QRRIRLVNTGVIAADSTALKRWLEHLGNDNAQGEYYLTDVFAAAADEYAAAEIVVVAD
                     PLETEGANDPWQLARLERAFQLRQVQALCTDGARVADPARIDIRGTVRVGRDVEIDVD
                     VVFEGEVELGDGVRIGPFCRIKDSRLAAGTEVRAHCDLDGARSEGAVQVGPYARLRPG
                     TVLADGAHIGNFVETKNATVGVGSKANHLTYLGDAVVGAGVNIGAGTITCNYDGANKF
                     TTTIQDNAFIGSNSALVAPVTIGQGATIAAGSVISKDAPAGELTVARARQVTLEGWVR
                     PVKQPKV"
BASE COUNT          903 a         1657 c         1717 g          634 t
ORIGIN      
        1 tcgaccagcc gcatgatgga tccgaacgtg atcggcaacg agcactacga gaccgcgcgc
       61 cgcgtgcagg gcaccctgca gaagtacaag gagctgaagg acatcatcgc gatcctgggc
      121 atggacgagc tgtcggaaga agacaagcag gccgtggccc gcgcgcgcaa gatcgagcgc
      181 ttcttcagcc agccgttcac cgtggccgaa gtgttcaccg gctcgaaggg caagtacgtg
      241 ccgctgaagg acaccatccg cggcttcaag ggcatcgtgg acggcgagta cgaccacctg
      301 ccggagcagg cgttctacat ggtcggctcg atcgacgaag ccgtcgagaa ggccaagaag
      361 ctcgccgcgt aaccacgtag gggcaggcct ccgcgcctgc ccatgaaagg aaagacatgg
      421 catccaccat ccgttgcgac atcgtcagcg ccgaggccga gatcttccac ggcgaggccg
      481 aactggtcgt ggccaccggc gaactcggcg agctgggcat cgcgcccaag cacgcgccgc
      541 tgatcacccg cctcaagccg ggcaaggtgg tcgtgaccct gccgggcggc gagaagctgg
      601 acttcgcgat cggcggcggc atcctcgagg tgcagccgac cgtggtcacc gtgctggccg
      661 acaccgccgt gcgggcgcag gacatcgacg aggccgcggt gcgtgcagcc aaggaagaag
      721 ccgagcgcat catcgcccac cgcggcgaga agatggacat cgccgaggcg cagcagaagc
      781 tggccgaagt cactgcgcag ctgcaggcgc tggagcgcct gcgcaagaac ctcaagcact
      841 gaggtgttcg cgacatcgca gcatcggaac gccggccttg tgccggcgtt ttttgttgct
      901 gccacgtcag tcaaacgggg actgaaaagg ggacggaggt ggttacggtg cgccttaacc
      961 acctccgtcc ccttttctcg tccaagcgaa gctaggtgcg cttgcgatac acccaatgcc
     1021 gcgacaacag gaagccgatg ccgccggaca gcaggtccgc gccgggcttg agcagctgcg
     1081 ccatgcgcag gccgaaggca tcatcgagat gcgcgacgat ccaggtgttg agcacggtca
     1141 gggcgagcca catgacgatg aagcggatcg ccgcatggcg accgacgtgg gtatcgtcgg
     1201 cagcgaacgt ccatttgccg ttgagccaga agccgagcat cgctccgcac aggcggccga
     1261 tcacgttggc cggctccacc ggcatcaccc attcgctcag cgcaagcatc aggccgtact
     1321 cgaccagcca ttgcagcagg ccgatgacgg cgtagccgcc caggtgccga cgcagcgtca
     1381 ccgcgcggtt tccatcggca gcgaccagcg ctggctgtcg gcggcttcgt cggtcttgcg
     1441 tgccttggcg atggcgagcc cggcgtgcag cgcggcactc gccttgcgat cgtcgaggat
     1501 cacgtggccg atgcgttcgc gttgcagcag cgacgcccac gcacggccgc tcgggtcggc
     1561 ttccacgcgc gcgaactcgg tttcccatgc cggcgaatac cagctcacgt tgcgaccgcg
     1621 ctggccgagt tcggcgagcg cgccgtagtt gccgggcagc accagcacca gcgtgccgtc
     1681 ggcgggcaac cgccgcgcca gcaggcgctc cggcgcatag cgcagcagca gcggtgcatc
     1741 gtccagcggg tggccgatgt cgcgcttcag cgcgccggtg cgcagcatcc agttggcatt
     1801 ggtggcgaag gcgaggttca acacgcacac gccccagaac gtgaccgcgc cgccgcgcag
     1861 cggcggccag gcgacgacca gtgccggcaa caacagcacc agcgacggct gcaggtaacg
     1921 cgcgtactgc agcggtagca gggtgagcag caggcccatg ctggcgacca ccgcgaggcc
     1981 gcgcgtgcgt cggtcgtgca atgcgagcag ccatgcgccg agcatcgcca ccagcacgaa
     2041 gccgaaaccg cccggatagg cttcgaaata gtggccggta tcgaatgtga tgtcccacac
     2101 gaggtcggca tcgaagccgc cgttccagcg cggatcgctg aaatcgcgtg gcgcgaagaa
     2161 cggcgagcgg aataggctgt tgaacaaagg cagcacggga ttgccggtca gcagccaggc
     2221 atgcacgtag ctcgcaccgc cacagatggc cgccagcaac gcgccgacgg gcaacgcgcg
     2281 ccacggcaag tgcgcgcgat gccgccacgc cgcccatgcg agcatgggca aggcgaccac
     2341 gccatgcatc ggcttgagcc cgcacagcgc accgaacagc agtgcgcctg cgagcacgga
     2401 gcggggcgaa gatgtcccgt cgtcgcgcag caccagccag gccagcgcgg gcagccacgc
     2461 catcgccacc agttcggtct gcatgccggc ggccaggtgc atggtcagcg gcaggctggc
     2521 catcaacgcc accgcccacc agcgccgcgt cgcgtcgccg ccgagtgccc cggccagtcc
     2581 gtgcatcgcg ccagcggcga cgatcagcca cagcgcattc atcgcgccgc gcgcttcccc
     2641 accggccagc acctgcgcca cgccctgcag cacgtcgccg ttccacgggg ccaatgccca
     2701 cacctgcagg cgcgcgtcca tcgcgtagcg cgcggtggcc tgcaactgcg agggcaggcc
     2761 gaggtgatag gcgacgtcgt ccgactgcat ggtcggcagc cacgcggcag tgctggcgag
     2821 gccgagcagc aacagtgcgc ccgttgccgc ggcaggcgat gcgcgcacgc cggcatcgaa
     2881 ggtgtgccaa gcgccccgca aggcatcgag gatcgcccgg cgtcgccacg cgaccactgc
     2941 gatgcaggcg agggcgtaca cggcgcgatg gtggatcggc agcgacagca gccagcccag
     3001 cgtgccggcg atcatcgcca aaccgatcgg cagcgcgatg ggaccgcgca gcaccaggct
     3061 gccaacgccg atcgcggctg ccgccagcaa ggcagtggcg agcaccggca tcggcccgga
     3121 aaacaccgcg agcgcgagca gccaggcggc gcccagcgct gccgcacgcg accactgccg
     3181 ccagcgttgc aagggccatg ccgccgcaag tgcgagcaag gcgagcagcc catgctcgaa
     3241 cgcacgcggg aatggcagtt cctgccacat gcgatgcgcg accagtccga ccacgcacag
     3301 cgcgatgccg gtccaggaca ggagggcggg gattggcgac gggtgcgtgc gctcgggcat
     3361 cggcgccatg ctaggcgctg gcagggaggc gtcaatggcg cgtgcgcggc gcctgagggg
     3421 cagccgttag aatcgcggca tccacagacg agccattcca tgagcgcacc cctgcatgtc
     3481 gttgtcctgg ccgccggcga aggcaagcgg atgaagtccg cgctgccgaa ggtgctgcag
     3541 aaggtcgccg ggcgaccgat gctggcgcac gccatcgatg ccgcccgcgc gctatcgccg
     3601 cagggtatcc acgtggtcta cggacatggc ggcgaggcgg tccgcgccgc gttcgccgac
     3661 caacccgacc tgcactgggc gcagcaggtg cagcaattgg gcacgggtca cgccgtgcag
     3721 caagcgatgc ccggcgtgcc ggacgatgtg cgcgtgctgg tgctgtacgg cgacgtgcca
     3781 ctgatcaccg cgcattcgct gcggcggctg atggatgcgc cgggacggct ggccgtgctt
     3841 gccgcggagc tgccggatcc gaccggttac ggccgcatcc tgcgcgatcc tgaaggccgc
     3901 gtggcatcga tcatcgagca gaaggacgcg gacgaggagc agcgccggat ccgcctggtc
     3961 aacaccggcg tcatcgccgc cgactccacc gcgctcaagc gctggctgga gcacctgggc
     4021 aacgacaacg cgcagggcga gtactacctg accgacgtgt tcgcggccgc ggcggacgaa
     4081 tatgccgcag cggaaatcgt ggtggtcgcc gatccgctgg aaaccgaagg ggcgaatgat
     4141 ccgtggcagc tcgcgcgcct ggagcgcgcg ttccagctgc gccaggtgca ggccttgtgc
     4201 accgatggcg cgcgcgtcgc ggatcccgcg cgcatcgaca tccgcggcac ggtgcgcgtc
     4261 ggccgcgatg tcgagatcga tgtcgacgtg gtgttcgagg gcgaagtcga actgggcgac
     4321 ggcgtgcgca tcggcccgtt ctgccggatc aaggacagca ggctggcggc cgggaccgag
     4381 gtccgcgcgc attgcgacct cgacggcgca cgcagcgaag gtgcggtgca ggtcggtccg
     4441 tatgcgcggc tgcgccccgg caccgtgctc gccgacggcg cgcacatcgg caacttcgtc
     4501 gagaccaaga acgcgacggt cggcgtgggc agcaaggcca atcacctgac ctacctcggc
     4561 gacgcggtgg tcggcgcggg cgtcaacatc ggcgccggca ccattacctg caactacgac
     4621 ggcgcgaaca agttcaccac gacgatccag gacaacgcct tcatcgggtc gaacagcgcg
     4681 ctggtggcgc cggtgacgat cggccagggc gcgaccatcg cggcaggatc ggtgatcagc
     4741 aaggatgcac cggcgggcga attgaccgtg gcgcgcgcgc gccaggtcac gctggaaggg
     4801 tgggtgcggc cggtgaagca accgaaggtg tgacgtccgc gatcgagaaa gtggagaaag
     4861 tggggtcaga gtcgactttc gcctgcgaaa gtcgactctg accccacttt c
//
LOCUS       DAOOCX010000167         4763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_16902_length_4763_cov_6.778250, whole genome shotgun sequence.
ACCESSION   DAOOCX010000167 DAOOCX010000000
VERSION     DAOOCX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16902_length_4763_cov_6.778250"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="PK399_11510"
     CDS             <1..1381
                     /locus_tag="PK399_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW13787.1"
                     /translation="DITDRRRIEQELRYLANYDTLTNLPNRTLLAERLSRAIVRARRQ
                     GSRMALLFLDLDRFKDINDSLGHATGDRILRATAQRLQEVVGDGHTVARIGGDEFTVL
                     LEDLSSGDEAERCAQRIIDAFDAPLRLDDRHEIAISPSIGISLFPDHAQVPTDLLKHA
                     DTAMYQAKAAGRRTFLRYADAMDGDIRRRATLIAALRKVVERGELRLVYQPQLVLADG
                     RIGAVEALLRWNSPEHGDVPPSQFIPLAEESGLIVSIGEWVLREACNTLADWRRDGID
                     NVLMSVNVSAVQLLRSDLAQTVQQVLRDTGVPANQLELELTESVLMANAEHATERLQT
                     FRRLGVSIAVDDFGTGYSSLAYLRRLPITTLKIDKAFIDDLGRPGDNEDAAITTTVIA
                     MAHSLGLRVVAEGVETAEQQRFLLQHGCDMVQGYLLSRPLNAEDCLRFLQEWPKQEPA
                     RLDPVASPP"
     gene            1391..1612
                     /locus_tag="PK399_11515"
     CDS             1391..1612
                     /locus_tag="PK399_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HPW13788.1"
                     /translation="MDNRDLHGELQRLLVRIDLLATRMQRLQDENRSLRQQHEQMATE
                     RAQLLAKQEQARSRVEAMISRLKSLEQHT"
     gene            1609..1926
                     /gene="zapA"
                     /locus_tag="PK399_11520"
     CDS             1609..1926
                     /gene="zapA"
                     /locus_tag="PK399_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPW13789.1"
                     /translation="MSTPESGKSEPVSIRVLDREYTVGVTEAERASLTSAARMLDARM
                     RELRGSNKMVAPDRLAVLTALNLAHELQQRDAVSQARSDGDLFKLIDAANARLDGLLA
                     SNT"
     gene            1997..2179
                     /gene="ssrS"
                     /locus_tag="PK399_11525"
     ncRNA           1997..2179
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK399_11525"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2227..2820
                     /locus_tag="PK399_11530"
     CDS             2227..2820
                     /locus_tag="PK399_11530"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011257970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPW13790.1"
                     /translation="MSSIPDRAFLRRQLRQRRRDLPATARIAAAEALSNHLLSLPFLP
                     ASGLVAGYWAMDGEIGLHVFQLRLPAALTYCLPLLHADDTLRFAPWRAGDPLVTNRFG
                     IPEPDIDPDAALPASEMAMLVLPLVGFDGRCNRLGMGGGWYDRSLAFRRDTSAPPWLV
                     GAGFDVQRVEALQHEAWDVPLDAVCSEVATHLAEHIA"
     gene            2817..3287
                     /locus_tag="PK399_11535"
     CDS             2817..3287
                     /locus_tag="PK399_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HPW13791.1"
                     /translation="MSKPKRYWMMKSEPDAFGIDDLERVGTEPWNGVRNYQARNFMRD
                     GMREGDGVMIYHSNCKVPGIAGIARVASAAYPDETQFDATSKYFDEKASREQPRWFLV
                     DVAFERKLKRLIPLEEIKTHTDQLGEGFALTAKGSRLSVFPVTSAQWKLLLSLE"
     gene            3292..3939
                     /gene="rpiA"
                     /locus_tag="PK399_11540"
     CDS             3292..3939
                     /gene="rpiA"
                     /locus_tag="PK399_11540"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HPW13792.1"
                     /translation="MSEAKRLAAVKAIDYVEDGMIVGVGTGSTVAYFIDALAPWRDRI
                     AGAVSSSEQSTARLKSHGIEVIDLNAAGTLSLYVDGADECDPHKRLIKGGGAALTREK
                     IIAEASKTFVCIVDPSKQVPILGKFPLPVEVIPMARSLVAREILRLTGGQPVWRDGTV
                     TDNGNVILDIHNLSIVDPVGVERELNQLPGVVSVGLFARRPADVVIIGGEPPIVL"
     gene            3978..4652
                     /locus_tag="PK399_11545"
     CDS             3978..4652
                     /locus_tag="PK399_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPW13793.1"
                     /translation="MRFSILAATLGYGLSMTAVGGTPADPVMRPPVEQLPPPLLHAKE
                     DLHALVARRDMDALLARVRPETKLDFGGGEGPDGFQSAWNSDQESRQRLWSTLEGILA
                     LPGVARDFDHGAEYCAPYVFCTGLPGDLDPFEALVVLGSGVAIRDRPSLSGTVLTRVD
                     HAVLTASDDPASVPTPDWTRVRLADGTAGYINNRWVRSPIDFRFVLRVDDGTDAWWLG
                     FLLAGD"
BASE COUNT          817 a         1657 c         1523 g          766 t
ORIGIN      
        1 cgacatcacc gatcgtcgcc ggatcgagca ggaactgcgc tacctggcca actacgacac
       61 cctgaccaac ctgcccaacc gcaccctgct ggccgaacgg ctgtcccgcg cgatcgtccg
      121 cgcgcgccgc cagggcagcc gcatggccct gctgttcctc gacctggacc gtttcaagga
      181 catcaatgat tcgctgggcc acgccaccgg cgaccgcatc ctgcgcgcaa ccgcgcagcg
      241 cctgcaggaa gtcgtcggcg acggccatac cgtggcccgc atcggtggcg acgaattcac
      301 cgtgctgctg gaggatctct ccagcggcga cgaggccgaa cgctgtgcac aacgcatcat
      361 cgacgccttc gacgcacccc tgcggctcga cgaccgccac gagatcgcga tctcgccatc
      421 gatcggcatc agcctcttcc ccgaccacgc gcaggtgccc accgacctgc tcaagcacgc
      481 cgacaccgcg atgtaccagg ccaaggcggc gggacggcgc accttcctgc gctacgccga
      541 tgcgatggac ggcgacatcc gccgccgtgc caccctgatc gccgcgttgc gcaaggtcgt
      601 cgagcgcggc gaactgcgcc tggtctacca accgcaactg gtgctggcgg atggccggat
      661 cggcgccgtc gaggcgctgc tgcgctggaa cagcccggag catggcgacg tgccgcccag
      721 ccagttcatc ccgctcgccg aggaaagcgg gctgatcgtg tcgatcggcg aatgggtgct
      781 gcgcgaggct tgcaacacgt tggccgactg gcgccgcgac ggcatcgaca acgtgctgat
      841 gtcggtgaat gtctcggccg tgcagctgct gcgcagcgac ctggcgcaga ccgtgcagca
      901 ggtgctgcgc gacaccggcg tgcccgcgaa ccaactggaa ctggaactca ccgaaagcgt
      961 gctgatggcg aacgccgaac acgcgaccga acgcctgcag accttccgcc ggctgggcgt
     1021 gtcgatcgcg gtggacgatt tcggcaccgg ctattcctcg ctggcctacc tgcgcaggct
     1081 gccgatcacc acgctgaaga tcgacaaggc cttcatcgac gacctcggcc gccccggcga
     1141 caacgaggac gcggcgatca ccacgacggt catcgcgatg gcgcattcgc tcggcctgcg
     1201 cgtggtcgcc gagggggtcg agaccgccga acagcaacgc ttcctgctcc agcacggctg
     1261 cgacatggtg cagggctacc tgctgtcgcg tccgctgaat gcggaggact gcctgcgctt
     1321 cctgcaggag tggccaaagc aggagccggc gcgccttgac ccggtcgcat cgcctcccta
     1381 aggtgcccgg atggacaacc gcgacctgca tggcgaactg caacggctgc tggtgcgcat
     1441 cgacctgctt gccacccgca tgcagcgcct gcaggacgaa aaccgcagcc tgcgccagca
     1501 acacgagcag atggccaccg aacgcgcgca attgctggcc aagcaggaac aggcgcgcag
     1561 ccgcgtcgaa gcgatgatca gtcgcctgaa gtcgctggag cagcacacat gagcacgccc
     1621 gagagcggca agagcgagcc ggtcagcatc cgcgtgctgg accgcgaata caccgtcggt
     1681 gtcaccgagg ccgagcgcgc cagcctgacc tcggccgcgc gcatgctcga tgcccgcatg
     1741 cgcgagttgc gcgggagcaa caagatggtc gcgccggatc gcctggcggt gctgaccgcg
     1801 ctgaacctgg cgcatgaact gcagcagcgc gacgccgtca gccaagcgcg cagcgacggc
     1861 gacctgttca agctcatcga tgcggcgaat gccaggctcg acggcctgct cgcgtcgaac
     1921 acgtgaaggc ctgaaccccg cgcgacccgc cgaccgatgg cgcttgccag cgacgacctc
     1981 gcgcctatac tggttgcgtc ctctgccgtg agcgacggcg tgcgcaacat tcgccttgtc
     2041 ccttaatgac gaccacgggg gcgtactggt tgccgggagt gcaagtccgc cccgagcggg
     2101 aagcccgatg gccgccacgc gttcccactt gaaccccggg ttcaaggtcg tttcgcacgc
     2161 atcgccacgg cggagacatt tttccgcgat gttcgaccct tccctgcagc acggcccgtc
     2221 cctcgcgtga gttccattcc cgaccgcgcc ttcctgcgcc gccagcttcg ccaacgccgg
     2281 cgcgacctgc cagccaccgc gcgcatcgcc gcggctgaag cactgtcgaa tcatttgctt
     2341 tcgctgcctt tcctgccggc atccggcctc gtcgccggtt actgggcgat ggacggggaa
     2401 atcggcctgc acgtgttcca gttgcgcttg ccagctgcgc tgacgtattg cctgccgctg
     2461 ttgcatgcag acgacaccct ccgcttcgcg ccgtggcgcg cgggcgatcc gctggtgacc
     2521 aaccgcttcg gcatccctga acccgacatc gatcccgatg ccgccttgcc tgccagcgag
     2581 atggccatgc tggtgctgcc tctggtcggc ttcgacgggc gctgcaatcg attgggcatg
     2641 ggcggtggct ggtacgatcg cagcctcgcc ttccgccgcg acacatccgc gcccccgtgg
     2701 ctggtcggtg ccggcttcga cgtgcagcgc gtcgaagcgc tgcaacacga ggcttgggac
     2761 gtacccctgg atgcggtgtg cagcgaagtc gccacccatc tcgccgagca catcgcatga
     2821 gcaagccgaa acgctactgg atgatgaagt ccgagcccga tgcgtttggc atcgatgacc
     2881 tggaacgcgt cggcaccgaa ccctggaacg gcgtgcgtaa ctaccaggcg cgcaacttca
     2941 tgcgcgatgg catgcgcgaa ggcgacgggg tcatgatcta ccactccaac tgcaaggtac
     3001 ccggcatcgc cggcatcgcc cgcgtggcca gtgccgcgta tccggacgag actcagttcg
     3061 atgcgacgtc caagtacttc gacgaaaaag ccagtcgcga acaaccgcgc tggttcctgg
     3121 tcgacgtggc cttcgaacgc aagctcaagc ggctgatccc gctcgaggag atcaagacgc
     3181 ataccgacca gctcggcgag ggcttcgcgt tgaccgcgaa gggcagtcgc ctgtcggtgt
     3241 tccccgttac ctccgcgcaa tggaaattgc tgctttccct ggaatgacga gatgagcgaa
     3301 gcgaagcgac tggcagccgt gaaggcgatc gactacgtcg aggacggcat gatcgtcggc
     3361 gtgggcaccg gttccacggt ggcctatttc atcgacgcgc tggcgccatg gcgcgaccgc
     3421 atcgccggcg cggtctccag ttccgagcag agcaccgcgc gactgaagtc gcatggcatc
     3481 gaggtgatcg acctcaacgc cgccggcacc ctctcgctgt acgtggacgg cgccgacgaa
     3541 tgcgatccgc acaagcgcct gatcaagggc ggcggcgcgg cgctgacccg cgagaagatc
     3601 atcgccgagg ccagcaagac cttcgtctgc atcgtggacc ccagcaagca ggtgccgatc
     3661 ctcggcaagt tcccgctgcc ggtggaagtg atcccgatgg cgcgcagcct ggtcgcccgc
     3721 gagatcctgc gcctcaccgg gggccagccg gtctggcgcg acggcacggt caccgacaac
     3781 ggcaacgtga tcctggacat ccacaatctg tcgatcgtcg atccggtggg cgtcgaacgc
     3841 gaactcaacc agctccctgg cgtggtcagc gtcggcttgt tcgcgcgtcg tccggcggac
     3901 gtggtgatca ttggcggcga gccgccgatc gtgctctgag cgaacgcgcc ccgagccttc
     3961 gcgagccgga tccggcgatg cgtttcagca tccttgccgc caccctcggg tacggcttgt
     4021 cgatgactgc cgtcggcggc acgcccgccg atccggtcat gcgtccgccc gtggaacagc
     4081 tgccgcctcc gctgcttcac gcaaaggaag acctgcacgc cctcgtcgcg cggcgcgaca
     4141 tggatgcgct gcttgcgcgg gtccgtcctg aaaccaaatt ggatttcggc ggcggcgaag
     4201 gaccggacgg attccagtcg gcatggaaca gcgaccagga atcgcgccag cgcctgtggt
     4261 cgacgctgga agggatcctc gccttgcccg gcgtggcgcg cgacttcgat cacggtgccg
     4321 agtattgcgc gccctacgtg ttctgcaccg gcttgcccgg tgacctcgat cctttcgaag
     4381 cgctcgtcgt cctgggctcc ggcgtggcga tccgggatcg cccttcgtta tccggcaccg
     4441 tgctcacgcg agtcgatcat gcggtgctga cggcaagcga tgatcccgcc agcgttccga
     4501 cgccggactg gacccgggtc aggctcgccg atggcacagc cggctacatc aataaccgct
     4561 gggtacgcag ccccatcgat ttcaggttcg tgttgcgggt ggacgatggc accgacgcct
     4621 ggtggctcgg tttcctgctg gccggcgact gagcgcatcc atcaggccgg cgatcaacgc
     4681 aggttgcgga tcgcgtccag cgcgatgtgg ggacactatt aacttgtccc gcttgcgcgg
     4741 acggccccaa aaagacaaaa ccc
//
LOCUS       DAOOCX010000168         4740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_17020_length_4740_cov_4.290928, whole genome shotgun sequence.
ACCESSION   DAOOCX010000168 DAOOCX010000000
VERSION     DAOOCX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17020_length_4740_cov_4.290928"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PK399_11550"
     CDS             <1..663
                     /locus_tag="PK399_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW13794.1"
                     /translation="ARPGTVTAIVGRSGSGKSSLVKLIPRFYEPSDGSILLDGHLLDD
                     YPLADLRRQIALVGQQVMLFDGSVAGNVAYGELQEADPAKIEAAVRDANALEFVARMP
                     DGLDTQIGAGGGKLSGGQRQRLAIARAMLKDAPILILDEATAALDNASERLVQDALAK
                     LIPDRTTLVIAHRLSTIEHADQVLVLDDGRLVEQGTHVELLARGGVYAHLHAMQFREG
                     AA"
     gene            660..1679
                     /gene="lpxK"
                     /locus_tag="PK399_11555"
     CDS             660..1679
                     /gene="lpxK"
                     /locus_tag="PK399_11555"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPW13795.1"
                     /translation="MSRKPLPKTPAWWHDGSTPPWHARLLATVYGNLSALRRSAYRRG
                     WLRRLKAGAPVVVVGNITAGGTGKTPLVIALVARLRAEGWTPGIASRGHGRDDEATPR
                     WVEPDSAASQVGDEPLLIARATGAKVRVDRDRVAAARALVAAGCDVIVCDDGLQHYRL
                     ARDIEIEVQDARRRYGNGRLLPAGPLREPVERAADCDFRVLNLGVATDADAQRASGFG
                     EWPMRLQVGAAQPLHGGRAQPLSAFTGQRVHAVAGIGDPERFFAMLRDAGIAVVPHAF
                     PDHHAYVAADFAFGSRLPVLMTVKDAVKCAELVDNQAFMVPVDAVLPEAFWVALLERL
                     PARGL"
     gene            1876..2658
                     /gene="kdsB"
                     /locus_tag="PK399_11560"
     CDS             1876..2658
                     /gene="kdsB"
                     /locus_tag="PK399_11560"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPW13796.1"
                     /translation="MTTEDFVVAIPARYAASRLPGKPLRLIGGEPMVLHVARRALAAG
                     AREAWVATDDARIAEALQGSGVQVAMTSPEHASGTDRLAECSRIAAWADDTIVVNLQG
                     DEPFAPADGIACVARTVADSGAGIATLATPIDDIETLLDPNAVKVVRAANGDALYFSR
                     APLPWPRDAFARDDRRMPGGGHWLRHIGIYGYRAGMLRAFAALPAGTLEAIESLEQLR
                     ALEAGWRIAVGLAPSPFPPGVDTPEDLERAERIHQAKEGNRT"
     gene            2682..3137
                     /locus_tag="PK399_11565"
     CDS             2682..3137
                     /locus_tag="PK399_11565"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HPW13797.1"
                     /translation="MVCLGNICRSPMAEGVLRARLEEAGLADKVRVDSAATGRSHIDH
                     APDERAIACAKKHGIDISAQRARALEITDFDEFDFILCADRTILHDARVRKPRTAHAD
                     VELLLEWSGIGKKDVPDPYYGDARDFEKAFKLVDSAAMGVVERIRQGRF"
     gene            3163..>4740
                     /gene="uvrC"
                     /locus_tag="PK399_11570"
     CDS             3163..>4740
                     /gene="uvrC"
                     /locus_tag="PK399_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPW13798.1"
                     /translation="MSKRKAGAGTPEFDGKAVAAAMPAAPGVYRMYSAEDGVLYVGKA
                     RALRNRVGSYFNATPKPTRIMAMLAQVARIEVTVTRSEADALLLENQLIKSLAPRYNV
                     MLRDDKSYPFVLVTKEDWPRIGFHRGPRGVAGRYFGPYPSAASVRETLNLMHKLFRLR
                     SCEDSVFRNRSRPCLQHQIGRCSAPCVGLVEAAEYAEAVRRAGLFLDGRSGELGDELT
                     SAMQAASEALEFEQAARLRDLITTIRKLQARQYVDGRNADLDVLAVAMQGASACVLLL
                     AFRDGRNLGTRAFFPKTNGEDSAAEVMAAFVSQYYAEQPPPTEIVLDRDIPERELIEQ
                     ALTEHAGRKVELRSNVRGERAAHLQLVTRNAELSLATELTSSSAQQARVEGLRELLGL
                     AELPARIECFDISHTMGEATVASCVVFDAAGPVRNQYRRYNIAGIEPGDDYAAMHQAL
                     SRRFRPAAEGEPDIILPDVLLIDGGAGQVAQAQAVLQDAGLQGIALVGVAKGPERKAG
                     AETLLLPDGSEVRPGAAS"
BASE COUNT          734 a         1621 c         1668 g          717 t
ORIGIN      
        1 gccaggccgg gcacggtgac cgccatcgtc ggccgctccg gcagcggcaa gtcgagcctg
       61 gtcaagctga tcccgcgctt ctacgagccc agcgacggca gcatcctgct cgatggccac
      121 ctgctggacg attacccgct ggccgacctg cgccggcaga tcgcgctggt cgggcagcag
      181 gtgatgctgt tcgatggcag cgtcgccggc aatgtcgcct acggcgaatt gcaggaggct
      241 gatcccgcca agatcgaagc cgcggtccgc gatgcgaatg cgcttgagtt cgttgcgcgc
      301 atgccggatg gcctggacac ccagatcggt gccggtggcg gcaagttgtc gggcggccag
      361 cgccagcgcc ttgccatcgc ccgcgcgatg ctgaaggacg cgccgatcct gatcctcgat
      421 gaagccaccg ccgcgctaga caacgcctcc gagcgactgg tgcaggacgc attggcaaaa
      481 ttgatcccgg atcgcaccac cctggtgatc gcgcaccggt tgtcgaccat cgaacacgcc
      541 gaccaggtgc tggtgctgga tgatggccgg ctggtggagc agggcacgca cgtcgagttg
      601 ctcgcgcgcg gcggcgtcta cgcgcacctg cacgcgatgc agttccgcga gggcgcggct
      661 tgagccgcaa gccgttgccg aaaaccccgg cgtggtggca tgacggcagc acgccgcctt
      721 ggcacgcgcg cctgttggca acggtctacg gcaatctctc cgccttgcgc cgcagcgcct
      781 atcgtcgtgg ctggttgcgt cggctgaagg cgggtgcgcc ggtggtggtg gttggcaaca
      841 tcaccgccgg tggcaccggc aagaccccgc tggtgatcgc cctggttgca cgcctgcgcg
      901 ccgagggctg gacccccggc atcgccagcc gcgggcatgg tcgcgacgac gaagccacgc
      961 cacgctgggt cgagccagac agcgctgcat cgcaggtcgg cgacgaaccc ctgctgatcg
     1021 cccgcgcgac cggcgcgaag gtgcgcgtgg atcgcgatcg cgtcgccgcc gcccgggcgc
     1081 tggtggccgc cggctgcgac gtcatcgtct gcgacgacgg cctgcagcat taccggcttg
     1141 cccgcgacat cgaaatcgag gtgcaggacg cgcgccgccg ctacggcaac ggccgcctgc
     1201 tgccggccgg gcccttgcgc gaaccggtgg aacgcgccgc cgattgcgat ttccgcgtcc
     1261 tcaacctggg tgtcgccacc gatgccgacg cacagcgcgc ttccggcttc ggggaatggc
     1321 cgatgcgcct gcaggtcggt gccgcccagc ccctgcatgg cgggcgcgcg cagccgctgt
     1381 ccgcattcac cggccagcgc gtgcatgcgg tcgccggcat cggcgatccg gagcgcttct
     1441 tcgcgatgct gcgcgatgcc ggcatcgcgg tcgtcccgca tgcattcccc gaccaccatg
     1501 cttacgtggc cgctgacttc gcgttcggca gccgcctgcc ggtgctgatg acggtaaagg
     1561 atgcggtgaa gtgtgccgaa ctcgtcgaca accaggcctt catggttccg gtcgatgcgg
     1621 tgttgcctga ggcgttttgg gtggccttgc tggagcggtt gcctgcgcga ggcctctgag
     1681 gccaggccgc ggctccggag gcgggtcgtc gtgttggcag ggggtggtcg ccgcttaggc
     1741 tccgcatccg gctccgatgc gcggccgcgc gccatccgtg gcgcgctcga ccaccccccg
     1801 ccaacacgac gacccctcgc tggtcatctg ctctcgcttc ggaaagagtg ggctgctgtc
     1861 ggcgataatg gagccatgac caccgaagat ttcgtcgtcg ccatccccgc ccgctatgcc
     1921 gcctcgcgcc tgcccggcaa gccgctgcgc ctgatcggcg gcgagccgat ggtcctgcac
     1981 gtcgcacgtc gcgcgctggc cgccggcgca cgcgaagcct gggtcgccac cgacgacgcc
     2041 cgcatcgccg aggccttgca gggcagcggc gtgcaggtcg cgatgacctc acccgaacat
     2101 gcatcgggca ctgaccggct ggcggaatgt tcgcggatcg ccgcttgggc cgacgacaca
     2161 atcgtggtca acctgcaggg cgacgagcct ttcgccccgg ccgacggcat cgcctgcgtg
     2221 gcgcgcaccg tcgccgacag cggcgccggc atcgcaacgc tggcgacgcc gatcgacgac
     2281 atcgaaacgc tgctcgaccc gaacgcggtg aaagtcgtgc gcgcggcgaa tggcgatgcg
     2341 ctctacttca gccgcgcccc gttgccatgg ccgcgcgatg ccttcgcccg cgacgaccgg
     2401 cggatgcctg ggggcgggca ctggctgcgc catatcggca tctacggcta tcgcgccggc
     2461 atgctgcgcg cgttcgcggc gttgccagcg ggaacgctcg aggccatcga atcgctggaa
     2521 caactgcgtg cgttggaggc cggctggcgg atcgcggtcg gcctcgcgcc atctcccttt
     2581 ccaccgggcg tcgatacgcc ggaggatctc gagcgcgcgg aacgtatcca tcaagcaaag
     2641 gaagggaacc ggacgtgagg gtcaggaaac ttggcgtgtt gatggtctgc ctcggcaata
     2701 tctgccgctc gccgatggcg gaaggcgtgc tgcgcgcgcg gctggaagaa gcggggctgg
     2761 ccgacaaggt gcgggtggat tcggcggcga ccggccggtc gcacatcgac catgcccccg
     2821 acgaacgcgc gatcgcctgc gcaaagaagc atggcatcga tatctccgcg caacgcgcgc
     2881 gcgctctcga aatcaccgac ttcgacgagt tcgatttcat cctctgcgcc gaccgcacga
     2941 tcctgcacga tgcccgcgtg cgcaagccgc gcaccgcgca cgccgatgtc gagttgctgc
     3001 tggagtggag cggcatcggc aagaaggatg tgcccgaccc gtattacggc gatgcacgcg
     3061 atttcgagaa ggcattcaag ctggtcgatt cggcagccat gggcgtggtc gagcgcatcc
     3121 gccagggacg cttctgagcc ccgcgggcat gggtagccgc gaatgagcaa gcgcaaggct
     3181 ggcgcgggca cgccggaatt cgacggcaag gccgtcgccg ctgcgatgcc cgccgcaccg
     3241 ggggtgtacc gcatgtacag cgccgaggac ggcgtgctgt acgtcggcaa ggcgcgtgcg
     3301 ctgcgcaacc gcgtgggcag ctacttcaac gccacgccca agccgacccg gatcatggcg
     3361 atgctggcgc aggtcgcgcg catcgaggtc accgtcaccc gtagcgaggc cgacgcgctg
     3421 ctgctggaaa accagctgat caagtcgctg gcgccgcgct acaacgtgat gctgcgcgac
     3481 gacaagagct acccgttcgt gctggtgacc aaggaggact ggccgcggat cgggttccat
     3541 cgcgggccgc gcggcgtggc tgggcgctac ttcgggccgt acccgagcgc ggcgtcggtg
     3601 cgcgagacgc tgaacctgat gcacaagctg ttccgcctgc gcagttgcga ggacagcgtg
     3661 ttccgcaacc gttcgcggcc atgcctgcag caccagatcg gccgctgcag cgcgccttgc
     3721 gtcggcctgg tcgaggccgc cgaatacgcc gaggccgtgc gtcgcgccgg gctgttcctc
     3781 gacgggcgca gcggcgaact cggcgacgaa ctgacgtcgg cgatgcaggc ggccagcgag
     3841 gcccttgaat tcgaacaggc ggcgcgcctg cgcgacctca tcaccaccat ccgcaagctg
     3901 caggcgcggc agtacgtcga tggccgcaat gccgacctcg acgtgctggc ggtggcgatg
     3961 cagggtgcat cggcctgcgt gctgctgctc gcattccgcg acggccgcaa cctcggcacc
     4021 cgcgcgttct tcccgaagac caatggcgag gacagcgccg ccgaggtcat ggccgcgttc
     4081 gtctcgcagt actacgccga gcagccgccg ccgaccgaga tcgtgctgga ccgcgacatc
     4141 cccgaacgcg aattgatcga acaggcgctc accgaacatg cgggccgcaa ggtcgaattg
     4201 cgcagcaatg tgcgcggcga acgcgcggcg cacctgcaac tggtgacgcg caatgcggaa
     4261 ctctcgctgg ccaccgagct gaccagcagc agcgcccagc aggcgcgggt ggaaggcctg
     4321 cgggagttgc tcggcctggc cgagttgccg gcgcggatcg aatgcttcga catcagccac
     4381 acgatgggcg aggccaccgt ggcgtcctgc gtggtgttcg atgcggccgg gccggtgcgc
     4441 aaccagtacc ggcgctacaa catcgcgggc atcgaaccgg gagacgacta cgccgccatg
     4501 caccaggcgc tgtcccggcg ttttcggccc gcagcggaag gcgagccgga catcatcctg
     4561 cccgatgtct tgctgatcga tggtggggcc gggcaggtgg cgcaggcgca ggcggtgctg
     4621 caggacgccg gtctgcaggg tatcgccctg gtcggggtgg cgaaggggcc cgaacgcaag
     4681 gccggggccg agaccctgtt gctgcccgac ggcagcgagg tccggccagg cgcggcatcg
//
LOCUS       DAOOCX010000169         4687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_17252_length_4687_cov_6.625216, whole genome shotgun sequence.
ACCESSION   DAOOCX010000169 DAOOCX010000000
VERSION     DAOOCX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17252_length_4687_cov_6.625216"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PK399_11575"
     CDS             <1..213
                     /locus_tag="PK399_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPW13799.1"
                     /translation="LEAWGEADPLNDRERQALRLAGEGMSAGEIAARLNLSQGTVRNY
                     LSEAIGKLGVGNRIEAYRLARQKGWL"
     gene            287..715
                     /locus_tag="PK399_11580"
     CDS             287..715
                     /locus_tag="PK399_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13800.1"
                     /translation="MKTVQRPTWFVLVAVLAVLWNLVGVFSFYTNLTAGPDVLATWPE
                     AQQRIAAATPRWVFVPFAIATIGGVLGSLGLLLGKRWAEPVLLLSLLGILVQFGSIYA
                     LTPTWALTGVGGAALPLGIAVVGVFLWWFAGKAVSRGWLR"
     gene            807..1319
                     /locus_tag="PK399_11585"
     CDS             807..1319
                     /locus_tag="PK399_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13801.1"
                     /translation="MIDVVRPCVLLAVATGTLLLAACGRGQPPPDRPEPPVASEPAPT
                     PAPPPAPVAPPAPPAAVAYKVEGVADAWHGVGIVCDFGKPFELKGGGLTVHFTPDSKD
                     GGSYRYDGTLQGFRVYGGEAYSVEWVGATPVTMKGWGVGSVDTPMGPRSSNGIEQYTI
                     ERSAEACPAA"
     gene            complement(1324..2703)
                     /locus_tag="PK399_11590"
     CDS             complement(1324..2703)
                     /locus_tag="PK399_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HPW13802.1"
                     /translation="MILPAGFAMPDPALTPWPLHIVDKAGFAAWRDAQSPAVQAWLAA
                     QRFDGSAGTAQSWADAHGAIGGAVIGIGDALDPYSYAHAPLALPTGDWQCVGDPDDAT
                     RHALQLGWGLGCYRFDRYKKSTRAPARLVDAFDGETLDLLAACVRVRDLVNTPTEHMG
                     PADLEAVARELAARHGAALTVTTGEDLLAQGYPAIHAVGRASHRAPRILELSWGDASH
                     PHVAICGKGVCFDTGGLDLKTAVGMRNMKKDMGGAAHALALAELVMARKLPLGLTVLV
                     PAVENAVGPDAFRPGEVVATRKGVSVEIDNTDAEGRMVLCDALARACELKPDLLLDFA
                     TLTGAARIALGPDLPVLYANDEALANDWLTAGNTTRDPLWRMPLWRPYLRYLTSGIAD
                     LANGSASTMAGSVTAALFLERFVADGQRWAHVDVYAWNDSDRAGKPAGGEAQGLRACY
                     AMLKARAAA"
     gene            complement(2700..3431)
                     /locus_tag="PK399_11595"
     CDS             complement(2700..3431)
                     /locus_tag="PK399_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPW13803.1"
                     /translation="MKPTIRAVTLDLDDTLWPFAPIGERIESALHDWFVAHSPSTAER
                     FPISEMRHLRERVFAQFPEHAHDLGLLRRLTIERALKESGDDPALATAAYAIFFRERN
                     RVDFYADAEAGLQRIAARLPVAGLTNGNADLGAVGIAPLFVFSLSAREYGAAKPDAGL
                     FHEACKRLGLPPHEVLHVGDDPRTDIAGAAAAGLQTCWIDRGDHAWPETLAPADLRVT
                     TLIELADALDAHLHPDATLRKEQAA"
     gene            complement(3498..4625)
                     /locus_tag="PK399_11600"
     CDS             complement(3498..4625)
                     /locus_tag="PK399_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPW13804.1"
                     /translation="MSTPPIDIATLPAAAPEPVPPDAAATTTRPRAPASLVVLAVLAV
                     GYTLWATQDLILPVLLAMFFALVGNPIIRVLQRIRIPRFLAALAVLCSGLALAILLGQ
                     QLVQPASEWIREAPRELRSLAPKLQKLAKPMQDANKAAENIARAAGGENTSRPVQVVR
                     TEVNDPYRVLTTTPRMVASILAVVLLTFFFMVYGQDLQRHAIALLPDRQKKRVTVEIL
                     HTIEIEVSRYVLTISVINMVVGLVFAACLYLGLDLPLDQALLWGTLAAILNFAPYVGP
                     LIGIVVMLLMGFSSFNDPVQSLLPAAIYLGLHTLEGQIVTPIVLGKRMALSPLILILA
                     LMVFGWIWGIIGLLLAVPMLVCVKIVLTRLDGMEGWAKLLE"
BASE COUNT          684 a         1596 c         1692 g          715 t
ORIGIN      
        1 ctggaagcgt ggggcgaagc cgatccactc aacgaccgcg aacgccaggc gctgcgcctg
       61 gcaggcgagg gcatgagcgc cggcgagatc gccgccaggc tcaacctgtc gcagggcacg
      121 gtgcgcaact acctgtccga ggcgatcggc aagctcggcg taggcaaccg gatcgaggcc
      181 tatcggctgg cacgccagaa gggctggctg tagcgctgtc gcagcgcggg atgcgccggg
      241 cacgcgagcg gcgtaggctg cgaccatcca accgggggga ttggcgatga agaccgtgca
      301 acgtccgacg tggttcgtgc tggtggccgt gctggcggtg ttgtggaacc tcgtcggcgt
      361 gttttcgttc tacacgaatc tcacggcagg tcccgatgtg ctcgccacct ggcccgaagc
      421 gcagcagcgg atcgcggcgg cgaccccgcg ctgggtcttc gtgccattcg ccatcgcgac
      481 catcggcggc gtgctggggt cgctcggact cttgctgggc aagcgctggg cggagccggt
      541 gttgctgctc tcgctgctcg gcatcctggt gcagttcggg tcgatctatg cgctcacccc
      601 gacctgggcg ttgaccgggg tcggtggtgc ggcgctgccg ttgggcatcg cggtggtcgg
      661 tgtgttcctg tggtggttcg ccggcaaggc tgtatcgcgt gggtggttgc gctgaggtgg
      721 caaacgaaca gcgcatggcc gaacgccacg ccgttttccg catccatcga tagaaccccg
      781 ttgcgggaca ccgtggaagc ctgtcgatga tcgatgtcgt tcgcccgtgc gtgctccttg
      841 ccgtcgccac cggcaccttg ctgctcgccg cctgtgggcg tggccagccc ccgccggaca
      901 ggcccgagcc tccggtcgcc agcgaaccgg caccaacgcc ggcacccccg cccgcacccg
      961 tcgccccacc tgccccgcct gccgccgtgg cgtacaaggt cgagggcgtc gccgacgcct
     1021 ggcacggcgt cggcatcgtg tgtgatttcg gcaagccgtt cgagctcaag ggcggcggcc
     1081 tgaccgtgca tttcaccccg gactcgaagg acggcggcag ctaccggtac gacggcacgc
     1141 tccagggatt ccgcgtgtac ggcggcgagg cctattcggt cgaatgggtc ggcgcgacgc
     1201 cggtcaccat gaagggctgg ggcgtgggct cggtggacac gccgatgggg ccgcgcagca
     1261 gcaacggcat cgagcaatac accatcgagc gatccgccga ggcctgcccg gcggcatgag
     1321 cgatcaggcc gcggcgcgcg ccttcagcat cgcatagcag gcgcgcaggc cctgcgcctc
     1381 gccgcccgca ggcttgccgg cgcggtcgct gtcgttccac gcatacacgt cgacatgcgc
     1441 ccagcgctgg ccgtcggcga cgaagcgctc caggaacagc gcggcggtca cgctgccggc
     1501 catggtgctg gcgctgccgt tggcgaggtc ggcgatgccg ctggtgaggt agcgcaggta
     1561 cggccgccac agcggcatcc gccacaacgg atcgcgcgtg gtgttgcccg cggtgagcca
     1621 atcgttggcc agtgcttcgt cattggcgta cagcaccggc aggtccgggc ccagtgcgat
     1681 gcgcgcggcg ccggtgaggg tggcgaagtc cagcagcagg tcgggcttga gttcgcaggc
     1741 gcgggccagc gcatcgcaca gcaccatccg cccttcggca tcggtattgt cgatctccac
     1801 gctcacgccc ttgcgggtgg cgaccacttc gccgggacgg aaggcatccg ggccgaccgc
     1861 gttctccacc gccggcacca gcacggtcag tcccagcggc agtttgcgcg ccatcaccag
     1921 ttcggccagc gccagcgcat gcgccgcgcc gcccatgtcc ttcttcatgt tgcgcatgcc
     1981 gaccgcggtc ttcaggtcca acccgccggt gtcgaagcag acgcccttgc cgcagatcgc
     2041 gacatgcgga tgcgacgcgt cgccccagct cagttcgagg atgcgcggcg cacggtgcga
     2101 cgcgcggccg acggcatgga tcgccgggta gccctgcgcc agcaaatcct cgccggtggt
     2161 gacggtcagc gccgcgccat gcctggccgc gagttcgcgc gccacggcct ccaggtcggc
     2221 cgggcccatg tgctcggtcg gggtgttgac caggtcgcgc acgcgcacgc aggcggccag
     2281 caggtcgagg gtttcgccat cgaacgcgtc gacgaggcgc gccggcgcgc gcgtgctttt
     2341 cttgtagcgg tcgaagcggt agcagcccag gccccagccg agttgcagcg cgtggcgcgt
     2401 ggcgtcatcg ggatcgccca cgcactgcca gtcgccagtg ggcagggcca gcggcgcatg
     2461 tgcgtagctg tagggatcca gcgcgtcgcc gatgccgatc accgcgccac cgatcgcccc
     2521 gtgcgcatcc gcccagcttt gcgcggtgcc ggcgctgccg tcgaagcgtt gcgcggccag
     2581 ccaggcctgc acggcgggcg actgcgcgtc gcgccacgcg gcgaagccgg ccttgtcgac
     2641 gatgtgcagc ggccagggcg tcagcgccgg atcgggcatg gcgaaaccgg cgggcaggat
     2701 catgcggcct gctccttgcg cagggtggcg tcgggatgca ggtgggcgtc gagcgcgtcg
     2761 gcgagttcga tgagggtggt cacgcgcagg tcggcgggcg caagcgtctc cggccaggca
     2821 tggtcgccgc ggtcgatcca gcaggtctgc aggcctgcgg cggcggcacc ggcgatgtcg
     2881 gtgcgcggat cgtcgccgac gtgcaggact tcgtgggggg gcaggccgag ccgcttgcag
     2941 gcttcgtgga acaggcccgc atccggcttg gccgcgccgt actcgcgcgc gctcagcgag
     3001 aacacgaaca gcggggcgat gccgaccgcg ccgaggtcgg cattgccatt ggtcaggccg
     3061 gccaccggca ggcgcgcggc gatgcgctgc aggccggctt cggcatcggc atagaaatcc
     3121 acccggttgc gttcgcggaa gaagatcgcg tacgccgcgg tggccagcgc cgggtcgtcg
     3181 ccgctttcct tcagcgcgcg ctcgatggtc agccggcgca gcaggcccag gtcatgcgcg
     3241 tgttcgggga actgcgcgaa cacccgctcg cgcaggtggc gcatctcgct gatcgggaag
     3301 cgctcggcgg tactcgggct atgggcgacg aaccagtcgt gcagggcgct ttcgatgcgc
     3361 tcgccgatcg gtgcgaaggg ccacagggta tcgtcgaggt cgagggtgac ggcgcggatg
     3421 gtaggcttca ccggcgcatt gtaggccgcg ccggcggcgc gggcgtgatc ctggtcgcgc
     3481 tttgccggcg cgggccgtca ctccagcagc ttggcccagc cttccatgcc gtccaggcgg
     3541 gtcagcacga tcttgacgca gaccagcatc ggcaccgcca acagcaggcc gatgatcccc
     3601 cagatccagc cgaacaccat cagcgccagg atcaggatca gcggcgacag cgccatccgc
     3661 ttgcccagca cgatcggggt gacgatctgc ccttccagcg tgtgcaggcc caggtagatc
     3721 gccgccggca gcagggactg caccggatcg ttgaagctgg agaaacccat cagcaacatc
     3781 accacgatgc cgatcagcgg gccgacatag ggcgcgaagt tgaggatcgc cgccagcgtg
     3841 ccccacagca gcgcctggtc cagcggcagg tccagcccca ggtacaggca ggcggcgaac
     3901 accaggccca ccaccatgtt gatcacgctg atggtcagca cgtagcggct gacttcgatc
     3961 tcgatggtgt gcaggatctc caccgtgacc cgtttcttct gccggtccgg cagcagcgcg
     4021 atcgcgtgcc gttgcaggtc ctgcccatag accatgaaga agaacgtcag cagtaccacc
     4081 gccaggatcg aggccaccat ccgcggggtc gtggtcagca cgcgataggg gtcgttgacc
     4141 tcggtcctga ccacctgcac cggccgcgac gtgttctcgc cgccggcggc gcgggcgatg
     4201 ttctcggcgg ccttgttcgc gtcctgcatc ggcttggcca gcttctgcag cttgggcgcc
     4261 aggctgcgca gctcgcgcgg ggcctcgcgg atccattcgc tggccggttg caccagctgc
     4321 tgccccagca ggatcgccag tgccaggccg ctgcacagca ctgccagcgc ggccaggaaa
     4381 cgcgggatgc ggatgcgctg cagcacgcgg atgatcgggt tgccgaccag ggcgaagaac
     4441 atcgccagca acaccggcag gatcaggtcc tgggtggccc atagggtgta gccgaccgcc
     4501 agcacggcca gtaccaccag cgaggccggc gcgcggggcc gcgtcgtcgt ggccgcggcg
     4561 tccggcggta cgggctcagg cgctgccgcg ggcagcgtcg cgatgtcgat cggcggcgtg
     4621 ctcatgcgcc gcgtgcggct tgttcgatgg cggcgatgtc ggcgctggtc accgccgcaa
     4681 cgccggc
//
LOCUS       DAOOCX010000170         4668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_17336_length_4668_cov_7.451116, whole genome shotgun sequence.
ACCESSION   DAOOCX010000170 DAOOCX010000000
VERSION     DAOOCX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17336_length_4668_cov_7.451116"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PK399_11605"
     CDS             <1..367
                     /locus_tag="PK399_11605"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301161.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPW13805.1"
                     /translation="PDLYRNVRPGADYQWSLTLLQQFKAQHPDIPTKSGIMLGLGETM
                     EQVQATLRDLRAHDVEMVTIGQYLQPSPAHHPLLRYWTPDEFKALEDYGYSLGFSHVA
                     SGPMVRSSYHADRQAAGIA"
     gene            552..>1711
                     /locus_tag="PK399_11610"
     CDS             552..>1711
                     /locus_tag="PK399_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPW13806.1"
                     /translation="MTSNRTLLALSLGLALAAPVALLARSADVASLPSAPTADQATTS
                     RLVYGLLSDSRYAYRPRALDDAMSKDILKRYLESLDPGKVFLTAQDVASFNRYATTLD
                     DAIKGGQVDPGWAIFAVYRQRVDQRIAHARKLLKGEFDFSADERYEFDREDAPWAASD
                     AELDALWTKSLRNDWLRLKLAGKQPDEIRKTLDKRYANLLSSVNELKGEEVFQTFINA
                     YAGAIDPHTDYLTPRSAENFTMQISNSLEGIGAVLFKQDDMIVVRELVPGGPAALSGK
                     LKSGDRIVGVGQGGSGEMKDVIGWRTDDAVELIRGTANTQVRLDIVSAEAPMDSKPTR
                     VVLTRAKVRLEDARAKAETITLPAADGQPARRIGVIKLPGFYQDFEARRRND"
     assembly_gap    1712..1811
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1812..2531
                     /locus_tag="PK399_11615"
     CDS             <1812..2531
                     /locus_tag="PK399_11615"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HPW13807.1"
                     /translation="GLIIGETSFGKGTVQNMVDLDRWPANEEPRFGQVKLTIAQFFRP
                     GGSSTQNKGVVPDVSFPTSVDASEYGESTYDNALPWTRIAAVPHVQYGNFAPLLGQLD
                     ARHDARAAKDVEFQWWVEDVRTFREEQAKKSISLNEAERRAERDRFDAKRKQRIEQRK
                     KLGIALDPLLEGRSDDGLQADERNVAESVAREEAAKKVTDPLLRESAAILGDAIGLLG
                     KDSKLAAQVLPEARTAGHWVD"
     gene            complement(2542..3042)
                     /locus_tag="PK399_11620"
     CDS             complement(2542..3042)
                     /locus_tag="PK399_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPW13808.1"
                     /translation="MTVPVVLDAFDHRLLELLQDDAGRTLTALGEAVGLSPSAVQRRL
                     KRYRAHGLLRQVAVLDAEAFPAVVLAAVWVTMERESVRQLNAFYARMRATPEVQQCYQ
                     LAGEWDYLVIMATTSMAAYRETAERLFKSDGNIKRYETRLVFDTVKRGLQLPTLAPAA
                     KGRRKR"
     gene            3111..4013
                     /gene="yedA"
                     /locus_tag="PK399_11625"
     CDS             3111..4013
                     /gene="yedA"
                     /locus_tag="PK399_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010379252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HPW13809.1"
                     /translation="MHTTSSAATPTGGRIALALAAVYVIWGSTYLAIRFALEGGFPPF
                     LLGGIRFLIAGSLMYAVLRWRGMRSPTRVQWGNALVLGILLMLLGNGMVNLAEQTVSS
                     GLAAVAVASAPLWMGVFSAMRGDRPTRREWIGLGIGFLGVLWLNAGSSLAATPMGMIA
                     LLVATLAWSFGSIWSRGRDLAPPFMNAAAQMLCGGAAMVVAGLVTGERLHALPTPHAL
                     GAFAYLVVLGSWFGFTAYIWLLHHVRPALAGSYAYVNPAIAVALGAWLAGERFGRHEL
                     VAMGVILLGVVAITFAKAAKGKPA"
     gene            4010..>4668
                     /gene="rarD"
                     /locus_tag="PK399_11630"
     CDS             4010..>4668
                     /gene="rarD"
                     /locus_tag="PK399_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005989311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HPW13810.1"
                     /translation="MRAAATNPAAQHRSGVWIAIAAYVAWGLMPLYWHLLKAVPAMQV
                     MAHRVVWSALFVCGWLGFKYGRGWVRETLSRPHAATMLALSGALIAVNWGLYIWAVNA
                     GHVVESSLGYFINPLLSVVLGVVVLKERLNRVQWLSVGIATMGVLWLTFSYGRFPWIA
                     IVLACSFGAYGLVRKLLGVPPVRGLGVESLWLLLPALAFLLWSEASGRGHLLPHAGQA
                     G"
BASE COUNT          696 a         1543 c         1583 g          746 t
ORIGIN      
        1 gccggatctc tatcgcaacg tgcgcccggg cgcggactac cagtggtcgc tgaccttgct
       61 gcagcagttc aaggcgcagc acccggacat cccgaccaag tccgggatca tgctgggcct
      121 gggcgaaacc atggagcagg tgcaggccac cctgcgcgac ctgcgcgcgc acgacgtgga
      181 gatggtcacc atcggccagt acctgcagcc gtcgcccgcc caccatccgc ttttgcgcta
      241 ctggacaccg gacgagttca aggcgcttga ggactacggc tactcgctgg ggttctcgca
      301 cgttgcgtcc gggcccatgg tgcgttccag ctatcacgcc gatcgacagg cggcgggcat
      361 cgcctgagct ggatcgcccc gcgcgccgtg ccgcgaatgc gcgcactggc gttgccggtt
      421 ccgttcacac agccttgcga aggcccgcta cagtgaggac gcaccctggc gcaccgaact
      481 tccggcactg tgccgcggtc gcagccaagc gcaccctgcc acgacggccc cccacgccgc
      541 ggaccccacg aatgacctcg aatcgcacct tgcttgccct ctcccttggc cttgccctgg
      601 ctgcgccggt ggccttgctg gcccgctccg ccgatgtcgc gtcgctgccg tccgcgccca
      661 ccgccgacca ggccacgacg tcgcgcctgg tctatggctt gttgtccgac agccgctacg
      721 cctaccgtcc gcgcgcgctc gacgacgcga tgtcgaagga catcctcaag cgctacctgg
      781 aatcgctgga cccgggcaag gtgttcctca ccgcccagga cgtggccagc ttcaaccgct
      841 acgcgacgac cctggacgac gccatcaagg gcggccaggt cgatccgggc tgggcgatct
      901 tcgcggtcta tcgtcagcgc gttgaccagc gcatcgccca tgcccgcaag ctgctgaagg
      961 gcgagttcga tttcagtgcg gacgagcgct acgaattcga ccgcgaggat gcgccgtggg
     1021 cggccagcga cgccgagctg gacgcgttgt ggacgaagtc gctccgcaac gactggctgc
     1081 gcctgaagct ggccggcaag cagcccgacg agatccgcaa gaccctcgac aagcgctacg
     1141 cgaacctgct ctccagcgtc aacgaactga agggagagga agtcttccag accttcatca
     1201 atgcctatgc cggcgcgatc gacccgcata ccgattacct gaccccgcgc agcgccgaaa
     1261 acttcacgat gcagatctcc aactcgctgg agggcatcgg tgcggtgctg ttcaagcagg
     1321 acgacatgat cgtggtgcgc gagctggtgc cgggcggtcc ggctgcgctg agcggcaagc
     1381 tgaaatccgg cgaccgcatc gtcggcgtcg gccagggcgg atcgggcgag atgaaggacg
     1441 tgatcggctg gcgcaccgat gacgcagtcg agctgatccg tggcaccgcc aatacccagg
     1501 tccgcctgga catcgtgtcc gccgaagcgc cgatggacag caagccgacc cgcgtggtcc
     1561 tgacccgcgc caaggtgcgc ctggaggatg cgcgcgccaa ggccgagacg atcaccctgc
     1621 cggctgccga tggccagccg gcgcgccgga tcggcgtgat caagctgccc ggcttctacc
     1681 aggacttcga ggcgcgccgc cgcaacgacg annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nggcctgatc atcggcgaga ccagcttcgg caagggcacc gtgcagaaca
     1861 tggtcgacct ggaccgttgg cccgccaacg aggagccgcg cttcggccag gtcaagctga
     1921 ccatcgccca gttcttccgg cccggtggca gcagcaccca gaacaagggc gtggtgccgg
     1981 acgtgagctt cccgaccagc gtggacgcca gcgagtacgg cgagagcacc tacgacaacg
     2041 cattgccgtg gacgcggatc gccgcggtgc cgcacgtgca gtacggcaat ttcgcgccgt
     2101 tgctgggcca gctggatgcc cgccacgacg cgcgcgcggc caaggacgtc gagttccagt
     2161 ggtgggtcga ggacgtgcgg accttccgcg aggagcaggc gaagaagtcg atctcgctca
     2221 acgaggccga gcgccgcgcc gagcgcgacc gcttcgacgc caagcgcaag cagcgcatcg
     2281 aacagcgcaa gaagctcggc atcgccctgg atccgctgct ggaaggccgc tccgacgacg
     2341 gcctgcaggc cgacgagcgc aacgtcgccg aatcggtggc gcgcgaggaa gcggcgaaga
     2401 aggtcaccga tccactgctg cgcgaatccg cggcgatcct gggcgatgcg atcggcctgc
     2461 tcggcaagga ttcgaagctg gcggcgcaag tgttgccgga agcgcgtacc gccggccact
     2521 gggtcgactg agtcgatcga ctcagcgctt gcgccggccc ttcgcggcgg gcgcaagcgt
     2581 gggcaactgc aatccgcgct tgacggtgtc gaacaccagc cgggtttcgt agcgcttgat
     2641 gttgccgtcg ctcttgaaca accgctcggc ggtctcgcgg taggcggcca tgctggtcgt
     2701 ggccatgatc accaggtagt cccattcgcc ggccagctgg tagcactgct gcacctccgg
     2761 cgtggcgcgc atgcgtgcgt agaacgcatt gagctggcgc accgattcgc gttccatcgt
     2821 cacccagacc gcggccagca ccaccgccgg gaaggcttcg gcgtccagca ccgcgacctg
     2881 ccgcagcagg ccatgcgcgc ggtagcgctt caatcggcgt tgcaccgcgc tgggcgacag
     2941 gccgacggcc tcgccgaggg cggtgagcgt gcggccggca tcgtcctgca ggagctccag
     3001 caggcgatgg tcgaaggcat ccaggacgac ggggacggtc acgcaaaaat attgcgccgc
     3061 tgcgcaggaa ttttcaaacc gcgtccgcgc gacaacgcta gcctgagtac atgcacacca
     3121 cctcttcggc cgccaccccc accggcggac ggatcgcgct ggcgctcgcc gcggtctacg
     3181 tcatctgggg gtccacctac ctggccatcc gtttcgcgct ggagggcggc ttcccgccgt
     3241 tcctgctcgg cggcatccgc ttcctcatcg ccggcagcct gatgtatgcc gtgctgcgct
     3301 ggcgcggcat gcggtcgccg acgcgcgtgc aatggggcaa cgcgctggtg ctgggcatcc
     3361 tgctgatgct gctgggcaat ggcatggtca acctggccga gcagaccgtc tcgtccggcc
     3421 tggccgcggt ggcggtggcc tcggcaccgt tgtggatggg cgtgttctcg gcgatgcgcg
     3481 gcgaccgtcc gacccgtcgc gaatggatcg gcctgggcat cggcttcctc ggcgtgctgt
     3541 ggctcaacgc cggcagcagc ctggcggcga cgccgatggg gatgatcgcg ctgctggtcg
     3601 cgacgcttgc gtggtcgttc ggttcgatct ggagccgcgg ccgcgacctc gcgccaccct
     3661 tcatgaatgc cgcggcgcag atgctctgcg gcggcgcggc gatggtcgtc gccggcctgg
     3721 tcaccggcga gcgcctgcat gccctgccga cgccgcatgc attgggtgcg ttcgcgtacc
     3781 tggtggtgtt gggttcgtgg ttcggcttca ccgcctacat ctggctgctg caccacgtgc
     3841 gcccggcgct ggccggcagc tatgcctacg tcaatccggc gatcgcggtg gccctgggcg
     3901 cgtggctcgc gggcgaacgc ttcggccgcc acgaactggt ggcgatgggc gtgatcctgc
     3961 tgggcgtggt cgcgatcacc ttcgccaagg ccgcgaaggg caagccggca tgagggcggc
     4021 cgccacgaac ccggccgcgc agcatcgaag cggcgtctgg atcgcgatcg ccgcctacgt
     4081 ggcatggggc ctgatgccgc tgtactggca cctgctgaag gcggtgccgg cgatgcaggt
     4141 gatggcccac cgcgtggtgt ggagtgcgct gttcgtctgc ggctggctgg gcttcaagta
     4201 cgggcgcggc tgggtgcgcg agaccctgtc gcggccgcac gcggcgacga tgctggcgct
     4261 gagcggtgcg ctgatcgcgg tcaactgggg cctctacatc tgggcggtga atgccggcca
     4321 cgtcgtcgaa tcctcgctgg gctatttcat caatccgttg ctcagtgttg tgctgggcgt
     4381 agtggtgttg aaggagcggc tcaaccgcgt gcaatggttg tcggtcggca tcgccaccat
     4441 gggcgtgctc tggctgacct tcagctatgg caggttcccg tggatcgcga ttgtgctggc
     4501 gtgttcgttc ggcgcgtacg gcctggtgcg caagctgctg ggcgtgccgc cggtgcgcgg
     4561 gctgggcgtg gagagcctgt ggctgctgct gccggcgctg gccttcctgc tgtggagcga
     4621 ggccagcggg cggggccacc tgttgccgca tgccggccag gcggggtg
//
LOCUS       DAOOCX010000171         4663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_17366_length_4663_cov_6.518880, whole genome shotgun sequence.
ACCESSION   DAOOCX010000171 DAOOCX010000000
VERSION     DAOOCX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17366_length_4663_cov_6.518880"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PK399_11635"
     CDS             <1..628
                     /locus_tag="PK399_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13811.1"
                     /translation="VPATSSPARIEATVEEWGLPGTFTQGTSLADLEARLGADNVRRV
                     KPDADTYACQAILFPDDPTRRAHVAFHDCERLDQLRSIEIRDRGSRWRGKHGVHVGMS
                     FEELRARNGKSFGFSGFDRDGIGRVHDQWSPAVGDDATLGKLDVEEGDRMYFDVMLGV
                     RADVPASARGSVPRDEYIASDDPRIPQLAPLLEVVGFGASTSLDDEWD"
     gene            complement(648..>1492)
                     /locus_tag="PK399_11640"
     CDS             complement(648..>1492)
                     /locus_tag="PK399_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW13812.1"
                     /translation="RLDFIINNACQTVRRPPAFYAHMMEGETAALQTMPAELRKLLGN
                     YEGLRSADLLPGADATALQAGRIEIAGAAGLTRAAELSQVPLLADELLGQAHLFPEGR
                     LDQDLQQVDLRGRNSWRLQMDEVPSVELLETQLVNAVAPFIINARLKPLMLRTADGEA
                     PSRDKHIVNVSAVEGQFYRNFKTTKHPHTNMAKAALNMMTRTAAADYHGDGIHMNSVD
                     TGWVTDEDPAEIAARKIVEERFHPPLDIVDGAARIVDPIIHGFNTGQHVWGQFLKDYA
                     PTDW"
     assembly_gap    1493..1592
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1593..1939)
                     /locus_tag="PK399_11645"
     CDS             complement(<1593..1939)
                     /locus_tag="PK399_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPW13813.1"
                     /translation="MNTTATPNSDTAAAVPHDDASPDNRLQPALSLLDAIIADRDVME
                     AWPAADRERLLQAVALVHHPEPRARRRKSKDLARERAQEKARATEALLDQTGIRTLRR
                     KPVFTTPNYFPPQA"
     gene            complement(2047..2601)
                     /locus_tag="PK399_11650"
     CDS             complement(2047..2601)
                     /locus_tag="PK399_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HPW13814.1"
                     /translation="MAKANPLQEQLLKAGLVKKSKVSEVAREQLKAKHGKAPAAASEI
                     QRDAERARAEKAERDRALEMERKAKARNAELAAQARQIIADKKVPRTGEIEYRFTADG
                     AIRTLLVDEALRKQLASGALVIARQGDRYELLPRAAGDKVRERDPGLIVLDHGQATGR
                     EAAASTSEDDAYYAQFQVPDDLVW"
     gene            2672..3169
                     /locus_tag="PK399_11655"
     CDS             2672..3169
                     /locus_tag="PK399_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HPW13815.1"
                     /translation="MSPLKYLAGHPPHVLERVHALIAEGRLGEMLAAKYDQAHAIRND
                     GQLYDYVQELKDRHLRKSVPLGRVLYDGKLQVMKDALGTHTTKYRVHGDRLKASREIR
                     IASVFRDAPAEFLKMIVVHELAHMKEAEHDKAFYRLCTHMAPDYHQLEFDLRLYLTDL
                     ETPRP"
     gene            complement(3228..>4663)
                     /locus_tag="PK399_11660"
     CDS             complement(3228..>4663)
                     /locus_tag="PK399_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW13816.1"
                     /translation="TVKRERLLPLIVATALFIENMDSTAIATSLPAIALDFGVEPVAL
                     KLALTTYMLALAVFIPVSGWVADRFGARPTFMVAIGVFLLGSIGCASADSLGALVAAR
                     FLQGMGGAMMVPVGRLVILRSIAKAQLVRALSWLTVPALLGPMMGPPLGGLITTYGNW
                     RYIFLINIPMGLLGIFLAWRHIPLLRGEPKPLDVRGFLLSAVGLALTMFGFASLGRHL
                     VSTELAAGCLLAGIAGLALYVLHARRHPHPLIDLGLLQVPTFSVGVIGGSLFRIGVGA
                     TPFLLPLMLQLSFGLDPLQSGLITFASAAGAMFMKTLAARILRRFGFRPVLVANALAA
                     SLLLCGFGLFRADTPYPLLIGVLLASGCFRSLQFTSLNAITYADIEPERMGQASSLAG
                     MAQQVALAIGVTIGGYALTVASLASGLPMDAAINFTFAFLTVGLVSASSAWMMLRLAP
                     DAGAEMSGKARPGEEVAEPKPLQRPGT"
BASE COUNT          726 a         1491 c         1623 g          723 t
ORIGIN      
        1 ggtgccggca acatcgtccc ccgcgcgcat cgaggcgacc gttgaggagt gggggttgcc
       61 cggtacgttc acgcagggca cctcactcgc ggacctggaa gcacgcctcg gtgccgacaa
      121 cgtgcgccgg gtgaagccgg atgccgacac ctacgcgtgc caagcgatcc tgttcccgga
      181 tgacccgacg cgccgtgcgc atgtcgcctt ccacgactgc gaacgactcg accagctgcg
      241 cagcatcgag atccgcgatc gcggatcgcg ctggcgcgga aagcatggcg tgcatgtggg
      301 catgtcgttc gaggaattgc gtgcgcgcaa cggcaagtcg ttcggcttca gcggcttcga
      361 ccgggacggc atcggccggg tgcacgacca gtggagcccg gcggtcggcg atgacgccac
      421 gctcggcaag ctggacgtgg aggagggtga ccgcatgtac ttcgacgtga tgctgggcgt
      481 gcgcgcggat gtacccgcgt cggccagggg cagcgtgccg cgcgacgaat acatcgccag
      541 cgacgatccg cgcatcccgc aactggcgcc gttgctcgaa gtggtcggct tcggcgcatc
      601 gaccagcctc gacgacgagt gggattgagc gcgcgcctgc tgctggctta ccagtcggtc
      661 ggggcgtagt ccttcaggaa ctggccccag acgtgctggc cggtattgaa accgtggatg
      721 atcgggtcga cgatgcgggc cgcgccatcg acgatgtcca gcggcgggtg gaagcgctcc
      781 tccacgatct tgcgcgcggc gatctcggcg ggatcctcgt cggtgaccca gccggtgtcg
      841 acgctgttca tgtggatgcc gtcgccgtgg taatcggcgg ccgcggtgcg cgtcatcatg
      901 ttcagcgcgg ccttggccat gttggtatgc ggatgcttgg tggtcttgaa gttccgatag
      961 aactggccct ccaccgcgga cacgttgacg atgtgcttgt cgcggctcgg cgcttcgcca
     1021 tcggcggtgc gcagcatcag cggcttcagc cgcgcgttga tgatgaacgg cgcgactgcg
     1081 ttgaccagtt gggtttccag cagttcgacc gacggcactt cgtccatctg caggcgccat
     1141 gaattgcgcc cgcgcaggtc gacctgctgc aggtcctggt cgaggcggcc ctcggggaac
     1201 agatgcgcct ggccgagtag ttcgtcggcc agcagcggca cctgcgacaa ctccgcggcg
     1261 cgggtgaggc cggccgcgcc cgcgatctcg atgcggcccg cctgcaatgc agtggcatcc
     1321 gcgccgggca acaggtcggc gctgcgcagg ccctcgtagt tgcccagcaa tttgcgcaac
     1381 tcggcgggca tggtctgcaa cgcagcggtt tcgccttcca tcatgtgcgc gtagaacgcg
     1441 ggcgggcggc gcacggtctg gcaggcattg ttgatgatga agtccaggcg ctnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcctgcgg cgggaaatag ttcggcgtgg
     1621 tgaacaccgg cttgcggcgc agggtgcgga tgccggtctg gtcgagcagc gcctcggtcg
     1681 cgcgtgcctt ttcctgcgca cgttcgcggg cgaggtcctt cgacttgcgg cggcgcgcgc
     1741 gcggttccgg atggtgcacc aacgccaccg cctgcagcag gcgttcgcga tccgccgccg
     1801 gccaggcctc catgacgtcg cggtcggcga tgatggcgtc gagcagggac agcgcgggtt
     1861 gcaggcggtt gtccggcgac gcgtcgtcgt gggggacggc cgctgcggtg tcggaattcg
     1921 gtgtcgcggt ggtgttcaag gacaggtccg tgttgcgcgc cgcaggggcg atggaatgag
     1981 gatgacgcga ggcgcgcgtc ggtcgatgcc cggcggacgg gcagggtgcg gtgcgtgatc
     2041 gcgcggttac cagaccaggt cgtcgggcac ctggaactgc gcgtagtagg catcgtcctc
     2101 ggaggtcgac gccgcggctt cgcgaccggt ggcctggcca tggtcgagca cgatcaggcc
     2161 gggatcgcgt tcgcgcacct tgtcgcccgc cgcgcgcggc agcagttcgt agcggtcgcc
     2221 ttgccgcgcg atcaccagcg cgccggaggc cagctgcttg cgcagggcct cgtcgaccag
     2281 cagggtgcgg atcgcgccat cggcggtgaa gcggtattcg atctcgccgg tgcgcggcac
     2341 tttcttgtcg gcgatgatct gtcgcgcctg cgcggccagt tcggcattgc gtgccttggc
     2401 cttgcgctcc atttccagcg cgcggtcgcg ctcggccttc tcggcgcgcg cgcgttccgc
     2461 gtcccgctgg atctcgctgg ccgccgccgg tgccttgccg tgcttcgcct tgagctgctc
     2521 gcgggcgacc tcggagacct tggatttctt gaccaggccc gccttgagca gttgttcctg
     2581 gagcgggttc gccttggcca tggtgcggtg ttctatcgtg ggtttccggc ccatcatacc
     2641 tgccgacccc tgcgcctcca ttcagccagc catgagcccc ctgaaatacc tcgccggtca
     2701 cccgccgcac gtgctggagc gggtgcatgc gctgatcgcg gaaggccgcc tgggcgagat
     2761 gctggcggcg aagtacgacc aggcgcatgc gatccgcaac gatggccagc tctacgacta
     2821 cgtgcaggag ctcaaggacc gccacctgcg caagtcggtg ccgctgggcc gggtgctgta
     2881 cgacggcaag ttgcaggtca tgaaggatgc gctgggcaca cacaccacca aataccgcgt
     2941 gcacggcgac cggctcaagg ccagtcggga gatccgtatc gccagcgtgt tccgcgatgc
     3001 gcccgccgag ttcctgaaga tgatcgtggt gcacgaactg gcgcacatga aggaagccga
     3061 gcacgacaag gcgttctatc gcctctgcac gcacatggca cccgactacc accagctgga
     3121 gttcgacctg cggctgtacc tgaccgacct ggaaacgccc cggccgtgat gagcgagcac
     3181 ctccgccggg cgaatcgaac cggcatgctt gcgggcgcgg tcgtggctca ggtgcccgga
     3241 cgctgcagtg gcttgggctc ggcgacttcc tcgcccggcc gtgccttgcc cgacatctcg
     3301 gcgccggcgt ccggggccag ccgcaacatc atccacgccg agctcgcgga gaccagcccg
     3361 acggtgagga aggcaaaggt gaagttgatc gccgcgtcca tcggcagtcc gctggccagg
     3421 ctggcgacgg tcagcgcata gccgccgatg gtgacgccga tggccagcgc cacctgctgg
     3481 gccatgccgg ccaggctgct ggcttggccc atgcgctcgg gttcgatgtc ggcataggtg
     3541 atcgcgttga ggctggtgaa ctgcagcgag cggaaacagc cactggccag cagcacgccg
     3601 atcaacagcg gatacggcgt gtccgcgcgg aacagcccga acccgcaaag cagcagcgag
     3661 gccgccagcg cattggcgac cagcaccggg cggaagccga agcggcgcag gatgcgcgcc
     3721 gccagggtct tcatgaacat cgcaccggcc gcggaggcga aggtgatcag tccggattgc
     3781 agtggatcca ggccgaagct gagctgcagc atcaacggca acaggaacgg cgtggcgccg
     3841 acgccgatgc ggaacagcga gccgccgatc acgccgacgc tgaaggtcgg gacctgcagc
     3901 aggcccaggt cgatcaacgg atgcggatgg cgacgcgcat gcagcacgta cagcgccagc
     3961 ccggcgatgc cggcgagcag gcagccggcg gccagttcgg tgctgaccag gtgccggccc
     4021 aggctggcga agccgaacat ggtcagggcc aggccgacag cggacaggag gaagccgcgc
     4081 acgtcgagcg gcttcggttc gcctcgcagc agcgggatat gccgccacgc caggaagatg
     4141 ccgagcagcc ccatcgggat gttgatcagg aagatgtaac gccagttgcc gtaggtggtg
     4201 atcaacccac caagcggcgg tcccatcatc ggcccgagca gcgcgggcac cgtgagccag
     4261 ctcagcgcac gcaccagctg cgccttggcg atgctgcgca ggatcaccag ccggccgacc
     4321 ggcaccatca tcgcgccgcc catgccctgc aggaagcgcg ccgccaccag ggcgcccagc
     4381 gagtccgcgc tagcgcagcc gatcgatccg agcaggaaca ccccgatcgc gaccatgaag
     4441 gtcgggcgcg cgccgaagcg gtccgcgacc cagccgctga ccgggatgaa caccgccagc
     4501 gccagcatgt acgtggtgag ggccagcttc agcgcgaccg gttcgacccc gaagtccagc
     4561 gcgatcgccg gcagcgaggt ggcgatcgcg gtggagtcca tgttctcgat gaacaatgcg
     4621 gtcgccacga tgagcggcag caggcgttcg cgtttcacgg tgc
//
LOCUS       DAOOCX010000172         4662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_17371_length_4662_cov_5.114608, whole genome shotgun sequence.
ACCESSION   DAOOCX010000172 DAOOCX010000000
VERSION     DAOOCX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17371_length_4662_cov_5.114608"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /gene="tssF"
                     /locus_tag="PK399_11665"
     CDS             <1..1852
                     /gene="tssF"
                     /locus_tag="PK399_11665"
                     /inference="COORDINATES: protein motif:HMM:NF017733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HPW13817.1"
                     /translation="CRGIAMLDELLPYYENELTYLRRLSKEFASKYPKIASRLQMEGE
                     VCEDPHVERLIESFSFLTARIHKKLDDEFPQITEAMLSVLYPHFLRPVPSMSIAQLQL
                     APGAEISTMQELPRHSQLLTRPVQGLPIRYRTGYPVDVWPIRIADARLEAAERSAFAV
                     TRTDTVATLRLRIEPTAQLPLSKLPIQRLRLYLDGESPLVHALHELLLNATASITLRA
                     PDDAPQVPPLRLPPSALKPVGFEEEDALLDYDPRSFLGYRLIQEYFVLPEKFLFIDVT
                     GLDLSRFDGAMEIAIELGAFGRPERLQRLEQTVTADTFRLFCTPIVNLFKQQGEPIRL
                     SQERHEYQVVPDVRRPLGLEVYSVDWVRKFSRTPTGNDSVEFYPAYSVQHGLHDDGSG
                     HYWYLQRRPSLLPNDDGSDVMLSLVDRDMNPRTPSVETLSVSLTCTNRDLPSALPFGG
                     DESLLQLEQGGVIASARLLKKPSATWRAPMRQANQWRLISHLSLNHLSIVDGGREALL
                     EILSLYNFADSSTLRKQIAGITRVGGKPSTTRVGQAPRQAFVRGTEIELEFDENQYVG
                     SGVYLMACVLDRFFGLYCTANSYTRLSVRTQQREDWLVRFAPRSGSLPLV"
     gene            1849..2946
                     /gene="tssG"
                     /locus_tag="PK399_11670"
     CDS             1849..2946
                     /gene="tssG"
                     /locus_tag="PK399_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HPW13818.1"
                     /translation="MSIESALPPPPHRDHVAGIDPMQPALERLHAEPGRFGFFQAVRL
                     LYSANGFDGRGTGSRPGPLRFTTPASLAFPPSELHSIETGDSNTRVCVNFMGLTGPSG
                     VLPRTYTELLIARRTLRDRSAQEFLDVFNHRLVSLFWLAWAKHRPEIGRQFGFQNSVL
                     RYLEYVVGLGTPALQARLHPNKRVATKTRELPGAALVYFSGLVAQRPHGERAIAQVVS
                     AVVGAPVQATGCLGTWQTIAGDARTRLGLDAHRLGHGCVLGTRYWDRQTTLRLTIGPL
                     DRARFNTLLPRGPRLGDVIELTRFLTGLAIDLRVRLSLRADQVPPLRLGARDQDRPQL
                     GWNTWLGGRRDPRPANDCEFHFSAMGGQSWR"
     gene            2937..>4662
                     /locus_tag="PK399_11675"
     CDS             2937..>4662
                     /locus_tag="PK399_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW13819.1"
                     /translation="MALNLRSLIARLNTTCRGAMEGAAGLCMSRGHYEVEVEHLLVKL
                     LEVENSDIHRILRQFEIAPEALERELGKSLDGFKTGNQRTPALSAMIPKLFESGWGWA
                     SIEFGEAKVRSGHLLVALLADNELRRLVSVSARSLEKINLETLTKNFHALTEASSEAR
                     DARALGDAGGAIGGDAGTPGSAPAGGKPSRTPNLDQYCINLTQRAREGKLDPVLGRDS
                     EIRLMVDVLTRRRQNNPILTGEAGVGKTAVVEGLALRIAQDDVPPQLRGVELMSLDLG
                     LLQAGASVKGEFENRLKGVIEEVKSSPKPIIMFIDEAHTLIGAGGAAGQNDAANLLKP
                     ALARGEMRTIAATTWSEYKKYFEKDPALTRRFQVVKVDEPDDEKAVSMLRGVGQAMAA
                     HHKVRILDEAITEAVKLSSRFIPGRQLPDKAVSVLDTACAKIAIGQNATPARLEDARR
                     RLDVLATERAQLEKEAAGGAEHGKRLGEIADETAKVSETLAADEARFAQEKELVGKIH
                     AELDKVEALPTDDPGRADAVATLNALRAELKALQGFEPMILPVVDSSAVAQIIAGWTG
                     IPVGRMVAD"
BASE COUNT          809 a         1623 c         1513 g          717 t
ORIGIN      
        1 atgccgcggg atcgccatgc tcgacgagct gctgccgtat tacgagaacg aactcaccta
       61 cctgcggcgg ttgtcgaagg agttcgcgtc caagtacccg aagatcgcgt cgcggctgca
      121 gatggaaggc gaggtctgcg aagacccgca cgtcgagcgg ctgatcgagt cgttctcgtt
      181 cctcaccgcg cgcatccaca agaagctcga cgacgagttc ccgcagatca ccgaggcgat
      241 gctgtcggtg ctgtatccgc atttcctgcg gccggtgccg tcgatgtcca tcgcccagct
      301 gcaacttgca ccgggcgccg agatcagcac gatgcaggaa ctgccgcggc acagccagct
      361 gctgaccagg ccggtgcagg ggctgccgat ccgctatcgc accggctatc cggtcgatgt
      421 ctggccgatc cgcatcgccg atgcgcggct ggaagcggcc gagcgctcgg ccttcgcggt
      481 gacccgcacc gacaccgtcg ccaccttgcg cctgcgcatc gaacccactg cgcagctgcc
      541 gctgtcgaag ctgccgatcc agcgcctgcg cctgtacctg gatggcgaga gcccactggt
      601 ccacgcgctg cacgaactgc tgctcaacgc caccgccagc atcaccctgc gcgcgccgga
      661 tgacgccccg caggtgccgc cgttgcgcct gccgccttcc gcactgaagc cggtcggctt
      721 cgaggaagaa gacgcgctgc tcgattacga cccgcgttcg ttcctgggct atcggctgat
      781 ccaggaatac ttcgtgctgc cggaaaaatt cctgttcatc gacgtgaccg ggctggacct
      841 gtcgcgcttc gacggggcaa tggaaatcgc catcgaactg ggcgcctttg gccgtcccga
      901 gcgcctgcag cgactcgagc agaccgtcac cgccgacacc ttccggctgt tctgcacgcc
      961 catcgtcaac ctgttcaagc agcaggggga accgatccgg ctgtcgcagg aacgccacga
     1021 ataccaggtg gtgccggacg tgcgccgtcc gctgggcttg gaggtctact cggtggattg
     1081 ggtgcgcaag ttcagccgca cgcccaccgg caacgattcc gtcgagttct acccggccta
     1141 ctcggtgcag cacggcctgc acgacgatgg cagcggccac tactggtacc tgcaacggcg
     1201 gccctcgttg ctgcccaacg acgacggctc ggacgtcatg ctcagcctgg tcgatcgcga
     1261 catgaacccg cgcacgccgt cggtggagac gctgtcggtg agcctgacct gcaccaaccg
     1321 cgacctgccc tcggccttgc ccttcggcgg cgacgagagc ctgctgcaac tcgaacaagg
     1381 cggcgtcatc gccagcgcgc ggctgctcaa gaagcccagc gcgacctggc gcgcaccgat
     1441 gcgccaggcc aaccagtggc ggctgatttc gcacctctcg ctcaaccacc tgtcgatcgt
     1501 cgatggtggc cgcgaggccc tgctggaaat cctcagcctc tacaacttcg cggactccag
     1561 caccctgcgc aagcagatcg ccggcatcac ccgcgtcggc ggcaagccct ccacgacgcg
     1621 ggtcggccag gcaccgcgcc aggccttcgt gcgcggcacc gagatcgaac tggagttcga
     1681 cgagaaccag tacgttggtt ccggcgtgta cctgatggcc tgcgtgctgg atcgcttctt
     1741 cggcctgtac tgcacggcga attcctatac ccgcctcagc gtgcgcaccc agcagcgcga
     1801 ggactggctg gtccgcttcg cgccgcgcag cgggagcttg ccgctggtat gagcatcgag
     1861 agcgcattgc cgccgccacc gcatcgcgac cacgttgccg gcatcgaccc gatgcagccc
     1921 gcgctggaac gcctgcatgc ggagccgggc cgcttcggct tcttccaggc agtgcgcctg
     1981 ctgtattcgg cgaacggctt cgatggccgc ggcaccggtt cgcgcccagg gccgctgcgc
     2041 ttcaccaccc cggcgtcgct ggcgttcccg ccatccgaac tgcattccat cgaaaccggg
     2101 gacagcaata cccgcgtgtg cgtgaacttc atgggcctca ccggcccatc cggcgtgctg
     2161 ccgcgcacgt acacggaatt gttgattgcg cggaggaccc tccgcgaccg cagcgcgcag
     2221 gaattcctgg acgtcttcaa ccatcggctg gtctcgctgt tctggctggc ctgggccaag
     2281 caccgcccgg agatcggccg ccaattcggc ttccagaatt cggtgctgcg gtaccttgaa
     2341 tacgtggtcg gcctgggcac acccgcactg caggcgcggc tgcacccgaa caagcgcgtc
     2401 gccacgaaga cgcgggaact accgggcgcg gcgctggtgt atttctccgg cctggtcgcg
     2461 caacgcccgc atggcgaacg cgcgatcgcg caggtggtgt cggcggtcgt cggtgcgccc
     2521 gtgcaggcca cgggctgcct gggaacgtgg cagacgatcg caggcgatgc gcgcacccgg
     2581 ctcggtctcg atgcgcatcg cctgggacac ggctgcgtgc tcggcacgcg ctactgggac
     2641 cggcagacca cgctccgcct gaccatcggc ccgctcgacc gcgcgcgctt caacacctta
     2701 ctgccgcgtg gcccacggct cggcgacgtc atcgaactca cccgcttcct cactggcctg
     2761 gccatcgacc tgcgcgtgcg cctgtcgctg cgcgccgacc aggtgccgcc gctgcggctg
     2821 ggcgcgcgcg accaagaccg cccgcaactg ggctggaaca cctggctggg cggtcgtcgc
     2881 gacccacggc cggcaaacga ctgcgaattc cacttttccg caatgggagg ccaatcatgg
     2941 cgctgaacct tcggtcgctg atcgcgcgac tcaacaccac ctgccggggc gcgatggaag
     3001 gcgccgccgg tctctgcatg tcgcgcggcc actacgaagt cgaggtggag cacctgctgg
     3061 tcaagttgct ggaagtcgag aacagcgaca tccaccgcat cctgcgccag ttcgagatcg
     3121 cacccgaagc gctggaacgc gaactcggca agtcgctgga cggcttcaag accggtaacc
     3181 agcgcacccc ggcactgtcc gcgatgattc ccaagttgtt cgagtccggc tggggctggg
     3241 cctcgatcga attcggcgag gcgaaggtgc gcagcggcca tctgctggtc gccttgctcg
     3301 ccgacaacga actgcgccgg ctggtcagcg tcagcgcgcg ttcgctggag aagatcaacc
     3361 tcgagaccct gaccaagaac ttccatgcgc tcaccgaagc ctccagcgaa gcccgcgatg
     3421 cacgtgcgct gggcgatgcc ggcggtgcga tcggtggcga cgcgggcacg cccggcagcg
     3481 cacctgcagg cggcaagccc agccgcacgc cgaacctcga tcagtactgc atcaacctga
     3541 cccaacgtgc gcgcgaagga aaactggatc cggtgctggg ccgcgattcg gaaatccgcc
     3601 tgatggtcga tgtgctgacc cggcgcagac agaacaaccc gatcctcacc ggtgaagccg
     3661 gcgtgggcaa gaccgcggtg gtcgagggcc tggccctgcg catcgcccag gacgacgtac
     3721 cgccgcaact gcgcggcgtc gaactgatgt cgctggacct gggcctgctg caggccggcg
     3781 cgtcggtgaa gggcgaattc gagaaccgcc tgaagggcgt gatcgaagag gtcaagtcca
     3841 gcccgaagcc gatcatcatg ttcatcgacg aggcgcacac cctgatcggc gccggtggcg
     3901 cggccggcca gaacgatgcc gccaacctgc tcaagccggc gctggcgcgc ggcgagatgc
     3961 gcaccatcgc ggcgacgacc tggtccgaat acaagaagta cttcgagaag gatcccgccc
     4021 tcacccgccg cttccaggtg gtcaaggtcg acgagccgga tgacgagaag gcggtatcga
     4081 tgttgcgcgg cgtgggccag gcaatggcgg cgcaccacaa ggtcaggatc ctcgacgaag
     4141 ccatcaccga ggcggtcaag ctgagctcgc gcttcatccc cggcaggcaa ctgccggaca
     4201 aggcggtcag cgtgctggat accgcctgcg ccaagatcgc gattggccag aacgccacgc
     4261 ctgcgcgcct ggaggatgca cgcaggcgac tggacgtgct cgcgaccgaa cgcgcgcaac
     4321 tggaaaagga agccgcgggc ggcgccgagc acggcaagcg cctgggcgag atcgcggacg
     4381 agaccgcgaa ggtcagcgaa acgctggccg cagatgaggc acgtttcgcc caggaaaagg
     4441 agctggtcgg caagatccac gcggaactcg acaaggtcga ggccttgccc accgacgacc
     4501 ccggtcgcgc cgacgcggtg gcgacgctca atgcgctgcg cgccgaactg aaggcactgc
     4561 aaggcttcga gccgatgatc ctgcccgtgg tggattccag cgcggtggcg cagatcatcg
     4621 ccggctggac cggcatcccg gtgggccgca tggtcgccga cg
//
LOCUS       DAOOCX010000173         4634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_17513_length_4634_cov_7.838174, whole genome shotgun sequence.
ACCESSION   DAOOCX010000173 DAOOCX010000000
VERSION     DAOOCX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17513_length_4634_cov_7.838174"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PK399_11680"
     CDS             complement(<1..959)
                     /locus_tag="PK399_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPW13820.1"
                     /translation="MNGVRVVPLPNGADEAQQPRGGVPAREALGRDELARLLDAAEQR
                     IQSLHRSERLQQALYEIADLAGGSLELQEMLRRIHAIVGELMYAGNLYIVLYDEHLQT
                     MRFLYFVDLIDPWVNDPAEEIPVTDDENTLTMHLLRTGEAMRGPSAELRQFHGIPFGE
                     SSGPDSADWLGVPMRRGSRVVGAIVVQNYEKPDVYSNEDRALLAYVAQHILTALDRYE
                     AREQLEKRVQERTAELQQANVDLQAEVAERQRAQELQRALFRIAELSMTSETLERFYA
                     EIHKVVGGLLYARNFYIAMLTIDGSMLEFPYSVDDRDQMRKPRR"
     gene            1118..1669
                     /locus_tag="PK399_11685"
     CDS             1118..1669
                     /locus_tag="PK399_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPW13821.1"
                     /translation="MDAPDTSDEALMLAYAAGDAAAFGELYARHRSRLYRYLLRQLRD
                     NALADEFFQDVWQRVVSARASWKPDAGFATWLYTIAHHRLGDHWRSLKHRPAAPSDAD
                     ERLARVTDPDTPERVLSEFERRRQLQLALDDLPEEQREVLLLRLEQELTLEEIGTITG
                     VGRETVKSRLRYAMDKLRAGLPA"
     gene            1666..2601
                     /locus_tag="PK399_11690"
     CDS             1666..2601
                     /locus_tag="PK399_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW13822.1"
                     /translation="MTRLPREPLDADERALAARLPRLHGREEPDAGLDARILAAAHAA
                     VSSQAPVAKRRRGWIVPLTAAASVSLAVGLAWQLQPPPGPPASTAHGPVASAVPGSNG
                     EQAVMRSTDAVPPPADAVQSLPSPMVAPAREAAPAEDSTRIARERTAAEPRAEAPAAT
                     PVFVPSPPPEAATADAMADAVMAPPPAPPAPPAPATPAARSPAAAPGRSAPVEGAVGA
                     AGNMVKPQAKQARAQDEAEAAAATTLDTVTEVVDEEDVPPATMDAPAARDAWLRRIGE
                     LQQQGRIDEAKASLAEFRRRYPDAVLPQELRKLEP"
     gene            2639..4396
                     /locus_tag="PK399_11695"
     CDS             2639..4396
                     /locus_tag="PK399_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPW13823.1"
                     /translation="MTSPTPAPKAPIGSLRALWPFVRRHSGLFMAWLLALACSSAATL
                     SLPVAFRSMIDHGFSSGANTDRTFGFVLLVILALALATAARYFFVTLLGERVVADLRT
                     RLYAHLLGLDAGFFDRSRSGELISRLTADAELLRSVVGSSMSVALRSIVTVIGALAML
                     FVTSPKLAAWALIGIPLAVLPIVVGSRRIQKVSRASQDRVADANALAAEALGAVRTVQ
                     AHAREAYERQRYATAIATAVATARKRIGAQSIVTAVAMSLIFGAIIVVLWSGAHDVIA
                     GRMSAGSLGQFVLYALIGGGSVGALAEVWNELQRAAGGMGRINELLQESSQVTAPATP
                     TTFSGRVRGEIVFDSVTFHYPQRPDTAALHDFSLRVRPGETVALVGPSGAGKSTLLSL
                     LLRFHDPQSGRITLDGIDLRELDLDALREAIALVPQSPTLFAASAADNIRYGRLDATD
                     EEVRQAAAAAEAETFLDALPDGFASELGERGTRLSGGQQQRVAIARALLKDAPVLLLD
                     EATSALDAQSERAVQTALDHLMEGRTTLVVAHRLATVLRADRIVVIDAGRIVAEGTHA
                     ALIAQGGLYAELARLQFLD"
     gene            complement(4468..>4634)
                     /locus_tag="PK399_11700"
     CDS             complement(4468..>4634)
                     /locus_tag="PK399_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPW13824.1"
                     /translation="GFGFISPEDGSADVFVHFSAIDSSGFRSLQEGQRVSYEVQQGAK
                     GAQAAGVKPI"
BASE COUNT          683 a         1695 c         1562 g          694 t
ORIGIN      
        1 cggcgcggct tgcgcatctg gtcgcggtcg tccaccgaat acgggaactc cagcatgctc
       61 ccgtcgatgg tcagcatggc gatgtagaaa ttgcgtgcgt agagcaggcc gccgacgacc
      121 ttgtggatct cggcatagaa gcgctccagc gtctcgctgg tcatcgacag ctcggcaatg
      181 cggaacagcg cgcgctggag ttcctgcgcc cgctgccgct ccgccacttc cgcctgcagg
      241 tcgacattgg cctgctgcaa ctccgccgtg cgctcctgca cgcgcttctc gagctgctcg
      301 cgcgcctcgt agcgatccag cgcggtgagg atgtgctggg ccacgtaggc cagcagcgcg
      361 cgatcctcgt tgctgtagac gtcgggcttc tcgtagttct gcaccacgat cgcgccgacc
      421 acgcggctgc cccggcgcat cggcacgccc agccagtcgg cgctgtccgg cccgctggat
      481 tcgccgaagg ggatgccatg gaactgtcgc agctccgccg acggcccccg catcgcctcg
      541 ccggtgcgca gcaggtgcat ggtcagggtg ttctcgtcgt cggtgaccgg gatttcctcg
      601 gccggatcgt tgacccaggg gtcgatcagg tcgacgaaat acaggaagcg catcgtctgc
      661 aggtgctcgt cgtacagcac gatgtagaga ttgccggcgt acatcagctc gcccacgatc
      721 gcgtggatcc ggcgcagcat ctcctgcaac tccaggctgc cgccggcgag gtcggcgatc
      781 tcgtacaagg cctgctgcag gcgctccgag cggtgcaggg actggatccg ctgctccgcc
      841 gcgtccagca ggcgggccag ttcgtcgcgg cccagcgcct cgcgcgcggg cacgccaccg
      901 cgaggctgtt gcgcctcatc ggcgccattc ggcaatggca cgacccggac cccgttcatt
      961 cggcgagcat acttgaacta cgcagcgcgg caccgcaccc gggcaacccc gttgtgcccg
     1021 cccgcccgtt cccgcctgcg tgacccatgc cctgcccgcc cccgccgtcc gccgcgcgcc
     1081 cttcccgcca gccacgccag tccgtacgct ggcgcccgtg gatgcccccg acaccagcga
     1141 cgaagcgttg atgcttgcct atgcggcggg cgatgcagcg gcattcgggg agttgtatgc
     1201 gcgccatcgc agcaggctgt atcgctacct gttgcgccag ttgcgcgaca acgccctggc
     1261 cgacgagttc ttccaggacg tctggcagcg cgtggtgtcg gcgcgcgcca gctggaaacc
     1321 cgatgcaggc ttcgccacct ggctgtacac catcgcccac caccgcctcg gcgaccactg
     1381 gcgcagcctc aagcaccggc cggccgcgcc atccgacgcc gacgagcggc tggcgcgcgt
     1441 caccgacccc gacacgcccg agcgggtatt gtcggaattc gagcgccgcc gccagttgca
     1501 actggcgctg gacgacctgc ccgaggaaca acgcgaggtc ctgctgttgc ggctggaaca
     1561 ggaactgacc ctggaagaga tcggcaccat caccggcgtg ggtcgggaga cggtgaagtc
     1621 gcgcctgcgc tacgccatgg acaagctgcg cgcggggctg ccggcatgac ccgtcttccg
     1681 cgcgagccgc tggacgccga cgagcgcgcg ctggctgcgc gcctgccgcg cctgcacggc
     1741 cgtgaagagc ccgacgcagg cctcgacgcg aggatcctgg ccgctgcgca tgcggcggtg
     1801 tcatcgcagg caccggtcgc gaagcgtcgg cgcgggtgga tcgtgccgct gaccgcggcc
     1861 gcctcggtca gcctggccgt gggcctggcc tggcaattgc agccgccacc aggaccgcct
     1921 gcgtccaccg cgcacggccc ggtcgccagt gcggtcccgg gcagcaatgg cgaacaggcg
     1981 gtgatgcgtt cgacggacgc ggtcccgccg cccgcggatg ccgtgcagtc ccttccatcg
     2041 ccgatggtcg cacccgcccg tgaggcagcg cctgctgagg acagcacgcg gatcgcccgc
     2101 gaacgcacgg cggcggaacc ccgcgccgaa gcccccgccg ccacgccggt cttcgtccca
     2161 tcgccgccgc cggaagcggc gacggccgat gcgatggccg acgcggtgat ggcaccgcca
     2221 cccgcaccac ccgccccccc ggcgccagcc acgccggcag cccggtcgcc ggcagccgca
     2281 ccgggacgat ccgcgcccgt cgaaggcgcg gtcggcgccg ccggcaacat ggtcaagccg
     2341 caggcgaagc aagcgcgggc gcaggacgag gccgaagccg cagcggcgac cacgctggac
     2401 acggtcaccg aggtcgtgga cgaggaagac gtgccgccgg ccacgatgga cgcaccggcc
     2461 gcgcgcgatg cctggctgcg ccggatcggg gaattgcagc agcagggccg gatcgacgag
     2521 gccaaggcca gcctggccga gttccgccga cgctatcccg atgcggtgct gccgcaggaa
     2581 ctgcgcaagc tcgaaccctg atgcccggcc gcggcaccac gccgctacca tgtgtcgcat
     2641 gacatccccc actccggcgc ccaaggcgcc catcggctcg ctgcgcgcgc tctggccgtt
     2701 cgtgcgccgc cattccggcc tgttcatggc ctggttgctg gcactggcct gttcctccgc
     2761 cgcgacgctc agcctgccgg tcgcgttccg gtcgatgatc gaccacggct tcagcagcgg
     2821 tgcgaatacc gaccgcacct tcggcttcgt gctgctggtg atcctggcgc tggcgctggc
     2881 gaccgccgcc cggtatttct tcgtgaccct gctcggcgag cgcgtggtcg ccgacctgcg
     2941 cacccgcctg tatgcgcacc tgctggggct ggacgccggc ttcttcgatc gcagccgcag
     3001 cggcgaattg atttcgcggc tcaccgccga cgcggaactg ctgcgcagcg tggtcggctc
     3061 gagcatgtcg gtcgccttgc gcagcatcgt gaccgtgatc ggtgcgctgg cgatgctgtt
     3121 cgtcaccagc ccgaaactcg cggcttgggc actcatcggc atcccgttgg cggtgctgcc
     3181 gatcgtggtc gggagccggc gcatccagaa agtctcgcgt gccagccagg accgggtcgc
     3241 cgatgccaat gcgctcgctg ccgaggcact cggcgcggta cgcacggtgc aggcccacgc
     3301 gcgcgaagcc tacgaacgcc agcgctatgc caccgccatc gcgacagcgg tggccaccgc
     3361 acgcaagcgc atcggcgcac agtcgatcgt caccgcggtg gcgatgagcc tgatcttcgg
     3421 cgcgatcatc gtggtgctgt ggtcgggtgc gcacgacgtg atcgccgggc gcatgagcgc
     3481 tggcagcctg ggccagttcg tgctgtatgc gctgatcggc gggggctcgg tgggtgcgct
     3541 ggccgaggtc tggaacgagc tgcaacgcgc cgccggcggc atgggccgga tcaacgagtt
     3601 gctgcaggaa tcgtcgcagg tcaccgcacc ggccacgcca accacgttct ccggacgcgt
     3661 gcgcggggag atcgtgttcg attcggtgac cttccactat ccgcaacgcc cggacaccgc
     3721 cgcgctgcac gacttcagcc tgcgcgtgcg gcccggcgag accgtggccc tggtcggccc
     3781 gtccggcgcc ggcaagagca ccctgctctc gctgttgctg cgtttccacg acccgcagtc
     3841 gggccgcatc accctcgatg gcatcgacct gcgcgaactg gacctcgacg cactgcgcga
     3901 ggcgattgcc ctggtgccgc agtcgccgac cctgttcgcc gccagcgccg ccgacaacat
     3961 ccgctacggg cgcttggacg cgaccgatga agaagtccgc caggccgccg cagccgccga
     4021 ggccgagacg ttcctcgatg cgctaccgga cggcttcgcc agcgaactcg gcgagcgcgg
     4081 aacgcggttg tcaggcggcc agcaacaacg cgtcgccatc gcgcgtgcgt tgctcaagga
     4141 cgcgccggtg ctgctgctcg atgaagcgac ctcggcactg gatgcgcaaa gcgagcgcgc
     4201 ggtgcagacc gccctggacc acctgatgga aggccgcacc accctggtgg tcgcgcatcg
     4261 actcgcaacg gtgttgcgcg ccgaccgcat cgtggtgatc gatgccgggc gcatcgtcgc
     4321 cgaaggcacc catgccgcat tgatcgccca gggcggcctg tacgcggagc ttgccaggct
     4381 gcagttcctc gactgaccgg cgcgtcgcgc cgccataaaa aagccccgca ccgggcgggg
     4441 ctcgaaatgg tgcgtgcgct gtcgcgatca gatcggcttg acgcccgccg cctgtgcgcc
     4501 cttcgcgccc tgctgcactt cgtagctcac ccgctggcct tcctgcaggc tgcggaagcc
     4561 cgaggaatcg atcgcggaaa aatgcacgaa gacgtctgca ctgccatcct cgggcgagat
     4621 gaagccgaag ccct
//
LOCUS       DAOOCX010000174         4492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_18210_length_4492_cov_7.144467, whole genome shotgun sequence.
ACCESSION   DAOOCX010000174 DAOOCX010000000
VERSION     DAOOCX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18210_length_4492_cov_7.144467"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PK399_11705"
     CDS             complement(<1..1023)
                     /locus_tag="PK399_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HPW13825.1"
                     /translation="MHSSRAAALLLALSTAWTLPSAAQAPAAPSPRPSATLATGACPA
                     IALAGADAGAVRVPSAADAWGGPRTGKEATLSNRTVAYSIDAELDPVKHTIKGKQALT
                     WRNRSAQPVCSLYVHMYLNGFEGPGSTFMTEQRASESGFRSNVDTKDGEFGFIRLDKV
                     VQDGAAAKWTFVQPDGGPKTDRSVVRIDLPRPVAAGATTTLDIDFFDQLPRVVARTGY
                     YGTFHLVAQWFPKVAVLELPGERGATAPRWNAHEFHLHSEFYADFGSYDVRLTVPKGY
                     TVGSTGALTGAPVEKAGKVTHRYVQHDVHDFAWTADNRYAKPLVKTWNGPNGPVQVSV
                     LYTPDYA"
     gene            1123..1476
                     /locus_tag="PK399_11710"
     CDS             1123..1476
                     /locus_tag="PK399_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HPW13826.1"
                     /translation="MTTHEGSCHCGAIAFTLEGDIGTVIDCNCSICRRRGHLLAFFPR
                     SAMTLQTPDADIASYGFNKHAIRHRFCPTCGVGPFGEGTHPKTGEATVAVNVRCLPGV
                     DLASLTVIPYDGASL"
     gene            1614..2495
                     /locus_tag="PK399_11715"
     CDS             1614..2495
                     /locus_tag="PK399_11715"
                     /inference="COORDINATES: protein motif:HMM:NF022431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HPW13827.1"
                     /translation="MRKRGWWMGLTLLACAPAMAGCPPMGQDRASLEALKSSGFAVAD
                     PDGKRALAMGLVECLGDPDPTLRDGIAYEALQHWMRAGDFDVAAVRGLRDALLARLDA
                     ADPHGVSRPFAALVLAEVARTDRIAPWMSPAEREAMLARATAYLASVRDYRGFEAGIG
                     WRHGVAHGADWLLQLSLNPALESVQLQRVLDAVALQAVPASGHAYVFGEAGRLAQPVA
                     YVARRGLLPEAAWQAWLDGLVARLGPMPPGGDAAWLARRHDLTAFLLALYLQSDTSQN
                     EALRALKPAIAKALAGG"
     gene            complement(2502..2801)
                     /locus_tag="PK399_11720"
     CDS             complement(2502..2801)
                     /locus_tag="PK399_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13828.1"
                     /translation="MPLLRIRLTGSEDDVRAISNLLQSLDGIEHVEEVADLMPRMDDA
                     DSSSAGLSDLQGPGAHEIEIDAPNPSTARRVREAVEALAFDLDLLVEFEEDGDGE"
     gene            2946..>4492
                     /locus_tag="PK399_11725"
     CDS             2946..>4492
                     /locus_tag="PK399_11725"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW13829.1"
                     /translation="MSATAATVSVESALAHGMRMLEQDPPLAAEQARQVLEASPGHPV
                     ARLLLGMAHNALDEPSPALALLQPLSVEQPSAPRVWMALGAAYAALDQRLEAMEALGH
                     AARLQPGLPRVWLQLAGLRDAGGDRAGAAEAYLAHTRNGIHDPALLAAGKALSAGRLP
                     QAEALLRERLRACPTDIAAMRMLAELAARIGRNEQAIELLQRCLELAPGFAMARHHYA
                     LMLDRGNRHQDALVEIDALLVDDPDNPALRNLKAVVLGKLGDYGDAIALYESILRERP
                     KDARVWMSLGHALKTEGKTERAISAYRRALELDPGFGSAWWSLANLKTVRFDARDIAA
                     MQAQLQRDGLADEQRLQVHFALGKALEDAGDHAASFAHYAQGNALRRAQLPYDAAQNS
                     QRRQRAQSVYTPAFFRERVGSGDPDQAPVFIVGMPRAGSTLVEQILSSHPQVEATMEL
                     PDLIAIVRDLRARSPRPEATSYHDIVAAMDPAEFRALGQRYLATSRVQRKLGRPFFID
                     KMPNNFA"
BASE COUNT          627 a         1523 c         1647 g          695 t
ORIGIN      
        1 ggcgtagtca ggggtgtaca gcacgctgac ctgcaccggc ccgttgggtc cgttccaggt
       61 cttgaccagc ggcttggcgt agcggttgtc ggcggtccag gcgaagtcgt ggacgtcgtg
      121 ctgcacgtag cggtgggtga ccttgccggc cttctccacc ggcgcaccgg tcagcgcacc
      181 ggtcgacccc acggtgtagc ccttgggcac ggtcaggcgc acgtcgtagc tgccgaagtc
      241 ggcgtagaac tcgctgtgca ggtggaactc atgggcgttc cagcgcgggg ccgtggcacc
      301 gcgctcgccc ggcagttcca gcaccgcgac cttgggaaac cactgcgcga ccaggtggaa
      361 ggtgccgtag tagccggtgc gcgcgaccac ccgcggcaac tggtcgaaga agtcgatgtc
      421 gagcgtggtg gtggcgccgg ccgcgaccgg gcgcggcagg tcgatgcgga ccacgctgcg
      481 gtcggtcttc gggccgccgt ccggctggac gaaggtccac ttcgccgccg cgccgtcctg
      541 caccaccttg tccaggcgga tgaagccgaa ctcgccgtcc ttggtatcga cattgctgcg
      601 gaagccgctc tcgctggccc gctgctcggt catgaaggtg ctgccgggac cctcgaagcc
      661 gttgaggtac atgtgcacgt acaggctgca caccggctgc gcgctgcggt tgcgccaggt
      721 cagcgcctgc ttgcccttga tcgtgtgctt gaccgggtcg agttcggcat cgatcgagta
      781 cgccaccgtc cggttcgaca gcgtcgcttc cttgccggtg cgcgggccgc cccaggcatc
      841 cgcggcgctg ggcacgcgga ccgcgccggc atcggcaccg gccagcgcga tggcggggca
      901 tgcgccggtg gccagggtgg cgctggggcg cggggaggga gcagcggggg cctgcgcggc
      961 ggacggcaac gtccaggcgg tgctgagcgc gagcaacaag gcggcggcac gggagctgtg
     1021 catggtctgg gtccggcggg aagcgaagca gaatccctag cgtgtgcgat ccgcgggggc
     1081 atggcaacat gccgccagtc acgcatccgg ggacatatcg ccatgaccac ccacgaaggc
     1141 agttgccatt gcggcgcgat cgcattcacc ctggaaggcg acatcggcac cgtcatcgac
     1201 tgcaactgct cgatctgccg ccgccgcgga cacctgctgg cgttcttccc gcgtagcgcg
     1261 atgaccctgc agacgccgga cgcggacatc gccagctacg gcttcaacaa gcatgcgatc
     1321 cgccaccggt tctgcccgac ctgcggggtc ggcccgttcg gcgagggcac ccatccgaag
     1381 accggcgagg ccaccgtcgc ggtcaacgtg cgctgcctgc ccggcgtgga cctggccagc
     1441 ctgaccgtga ttccgtacga cggcgccagc ctctgacccc gcgtcgacgc gtggccggct
     1501 tgatccggtg atgtgtttcc ggtgagggcg tgggttccgc cggtttcggt cgggtcgcga
     1561 ccgctcccgg catgcgcagg tggcaagtct cacgggacag gaaggggtgg gcgatgcgga
     1621 aacggggttg gtggatgggc ttgaccttgc tggcctgcgc gccggcgatg gcgggctgcc
     1681 cgccaatggg gcaggatcgg gccagtctgg aagcactgaa gtcgtccggc ttcgcggtgg
     1741 cggatccgga cggcaagcgt gcgctggcga tgggcctcgt cgagtgcctg ggcgaccccg
     1801 acccgacgct gcgcgacggc atcgcctacg aggcactgca acactggatg cgcgcgggcg
     1861 atttcgatgt ggctgccgtg cgtggcctgc gtgacgcgct gcttgcgcgc ctcgatgcag
     1921 cagacccgca cggcgtgtcg cggcccttcg ctgcgctggt gctggccgag gtcgcacgga
     1981 ccgaccggat cgcgccctgg atgtcgcctg ccgagcgcga ggccatgctg gcgcgggcca
     2041 cggcgtatct cgcttcggtc cgggactatc gcggcttcga ggccggtatc ggctggcgcc
     2101 acggggtggc ccatggcgcc gactggttgc tgcagttgtc gctgaacccg gcgctggagt
     2161 ccgtgcaact gcagcgcgtc ctggatgcgg tggcactgca ggcggtgccg gcctccggcc
     2221 atgcctatgt gttcggcgaa gccgggcggc tggcgcaacc ggtggcctac gtggcgcgcc
     2281 gcggcctgtt gccggaggcc gcgtggcagg catggctgga tgggcttgtc gcacgcctgg
     2341 ggccgatgcc gccgggtggc gacgcggcgt ggctggcgcg ccggcacgac ctcactgcct
     2401 tcctgttggc cctctacctc cagtcggaca ccagccagaa cgaggcgctg cgtgcgctga
     2461 agccggccat cgccaaggcg ctcgccggcg gctagcctgc atcactcgcc gtcgccgtct
     2521 tcctcgaatt cgaccagcag gtccaggtcg aaggccagcg cctcgacggc ttcgcggacg
     2581 cggcgtgcgg tcgatgggtt gggtgcgtcg atttcgattt cgtgggcgcc cggtccctgc
     2641 aggtcgctca ggccggccga gctggaatcg gcatcgtcca tgcgcggcat caggtcggcg
     2701 acttcctcga cgtgttcgat gccatcgagg ctctgcagga ggttggagat cgcgcggaca
     2761 tcgtcctcgc tgccggtcag gcggatgcgc aacaggggca tgggcgtggc ttccttgggg
     2821 ggacgcgcag gctaggtggc ggtcggcgaa gaccgggtga cggcgctgga cgctggcgca
     2881 tcgccgcggg tagattggac gggacgtgtc ggggggcgcg ccacatccat caccgcaggg
     2941 gtccgatgag cgccaccgca gcgaccgtca gcgtggagtc cgcgctcgcg catggcatgc
     3001 gcatgctcga gcaggacccg ccgctggccg ccgaacaggc acgccaggtc ctggaggcct
     3061 cgccggggca tccggtggcg cggttgctgc tggggatggc gcacaacgca ttggacgaac
     3121 cgtcgcctgc gctcgcgttg ctgcagcccc tgtcggtcga acagccctcc gcaccgcgtg
     3181 tgtggatggc gctgggcgcg gcctacgcgg ccctggacca gcggctggaa gcgatggagg
     3241 ccctgggcca tgcggcgcgc ctgcaacccg gcctgccacg ggtctggttg cagctcgcgg
     3301 gtttgcgcga tgccggcggc gaccgcgcag gcgccgccga ggcctacctg gcgcataccc
     3361 gcaacgggat ccacgatccg gcgttgctgg ccgccggcaa ggcgctgtcg gcgggccgcc
     3421 tgccgcaggc cgaagccctg ctgcgcgagc gcctgcgcgc ctgccccacc gatatcgcgg
     3481 ccatgcgcat gctggccgaa ctggcggcgc gcatcggccg caacgaacag gcgatcgaac
     3541 tgttgcaacg ctgcctggaa ctggcacccg gtttcgcgat ggcgcgccat cactacgcgc
     3601 tgatgctgga tcgcggcaat cgccaccagg acgcgctggt ggagatcgac gccttgctcg
     3661 tcgacgatcc cgacaatccc gccctgcgca acctgaaggc ggtggtgctg ggcaagctgg
     3721 gcgactacgg cgatgccatc gccctgtacg aatcgatcct gcgcgagcgg cccaaggacg
     3781 cccgcgtgtg gatgagcctg ggccacgcgc tcaagaccga gggcaagacc gagcgggcga
     3841 tttcggcgta tcggcgggca ttggagctgg atcccggctt cggcagcgcc tggtggagcc
     3901 tggccaacct caagaccgtg cgcttcgatg cgcgcgacat cgccgcgatg caggcgcaac
     3961 tgcaacgcga cggcctggcc gacgagcagc gcctgcaggt gcacttcgcc ctgggcaagg
     4021 cgctggagga cgcaggcgac catgcggcgt cgttcgccca ctacgcgcag ggcaacgcgc
     4081 tgcgccgcgc gcaactgccg tacgacgcgg cgcagaactc ccagcgccgc cagcgcgcgc
     4141 agtcggtgta cacgcccgcg ttcttccgcg agcgcgtcgg cagcggcgat cccgaccagg
     4201 ccccggtgtt catcgtcggc atgccgcgtg ccggttccac cctggtcgag cagatcctgt
     4261 ccagccaccc gcaggtcgag gccacgatgg agttgcccga cctgatcgcg atcgtgcgcg
     4321 acctgcgcgc gcgcagcccg aggccggagg ccacctcgta ccacgacatc gtcgcggcga
     4381 tggatcccgc ggaattccgc gcgctgggcc agcgctacct ggcgacgtcg cgggtgcagc
     4441 gcaagctggg ccggccgttc ttcatcgaca agatgccgaa caacttcgcc ca
//
LOCUS       DAOOCX010000175         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_18367_length_4465_cov_4.977551, whole genome shotgun sequence.
ACCESSION   DAOOCX010000175 DAOOCX010000000
VERSION     DAOOCX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18367_length_4465_cov_4.977551"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1495)
                     /locus_tag="PK399_11730"
     CDS             complement(113..1495)
                     /locus_tag="PK399_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW13830.1"
                     /translation="MPETPALLRPLWRRLLLSRPLWIGIVLLAIGAGAWWAWHAYHGR
                     PDLNEQYVFATVQRGDIQDLVTATGTVQPLDYVDVGAQVSGQLKKLHVEVGSTVQAGD
                     LLAEIDPTVLKATVDARRAGLRNQRATLAERESQRVLAELLLTRQRNLMKEDATTTES
                     LQQAEANAQAAEAQVDATRASIEQTESTLRADEANLNYARIYAPLAGTVVTVTARQGQ
                     TLNANQQAPTILRVANLSTMTVQTQVSEADVSKLRRGMEVYFTTLGSQGRRWYGALRK
                     VEPTPTVTNNVVLYNALFDVPNANQSLMTNMTAQVFFVAATAKDALLIPAAALNGGTR
                     PGDASGRRERSASEPRRGDRAASAAESPASASTSGFATETARGSRRATVRLLTAAGTL
                     EDRSVELGVSNRVQTQVLSGLAEGDQVVAGLKLPPAAPRNGSQSQPGSGLQQNPIGMP
                     PGMGGGQRSR"
     gene            complement(1683..2588)
                     /locus_tag="PK399_11735"
     CDS             complement(1683..2588)
                     /locus_tag="PK399_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW13831.1"
                     /translation="MTGMKRPREIDAGVLTVAYHESGPAEGPPVVLLHGFPYDVHAYR
                     DVAPRLAERGCRVIVPYLRGFGPTRFRSADTPRSGEQAALGADLLALLDALGVSRAVL
                     AGYDWGGRAACVVAALWPERCAGLLTLNSYNIQHISQAMQPEPPAQEHRLWYQYYFHS
                     ERGRAGLMQDRRAMCRLLWQQWSPTWVFDDATFDRSAAAFDNPDFVDVAIHSYRHRYG
                     LVAGDPAYAEIERRLAQQPAIRAPTISFDGADDGLFAPRDAAHHARHFTGPRTHRIAP
                     GAGHNLPQEAPDVFADAVLELVRER"
     gene            complement(2666..2977)
                     /locus_tag="PK399_11740"
     CDS             complement(2666..2977)
                     /locus_tag="PK399_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13832.1"
                     /translation="MNSLEQWISGITAAVCMVLLVRLCLGAARQRRFDHAVRALGWEL
                     RNRSQRLWRAPAARRAATREAQAAIDRARKGRGTSDADDGEWQGNVYTPKSFRKPRKP
                     H"
     gene            complement(3098..4366)
                     /locus_tag="PK399_11745"
     CDS             complement(3098..4366)
                     /locus_tag="PK399_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPW13833.1"
                     /translation="MKSNTAAIAADFSCANQHALATILDASQTQSIIAARDIFDISGT
                     KLWARDLPVSQALQRKLLDRQLREPLESCLKAADGVTAHTLVEAIERLLKQPTPLTAL
                     LHAHADTLLREAAKLPVHPVAQLLLTASRSSRPAAYEHATQAMALNGALMLAQGGKPT
                     DLRIAMLSGLLHDLGEMYIAPEFGEADAERTVDVLTYQQLVVHPHVGQLLIAQLTDYP
                     AEVARAIAEHHERMDGSGYPHCLRGDQLSMHGRLLAVTESTLATLSEPAAPLSRASIA
                     LRVVPGEYDLRWVGLITQVVRQQPTATAKRTAEELQERLTKLAEVMHDASTATATLRR
                     EATSAALKDALTLTEHLLARLQGGLMTSGLWSIDPVAAPDAAEVEAVEGELRLRLRRI
                     ERAVRLRAGLLTDEDARRLDDLCRQLTLRG"
BASE COUNT          631 a         1493 c         1608 g          733 t
ORIGIN      
        1 ttgcggtacg tcttggtgac gccgcgcaac tcgatcagcg gtgtcctgcc ctcggggtcg
       61 ggcggcatgg cgggctgctc cggcatcgcc gggtcgatgc ggtccacgga gatcaccgac
      121 tgcgctggcc gccgcccatg cccggcggca tgccgatcgg gttctgctgc aggccgctgc
      181 cgggctggct ctgcgagcca ttgcgcggtg ccgccggtgg cagcttgagc cccgcaacga
      241 cctgatcgcc ctcggccaga cccgacaaga cctgggtctg cacgcggttg ctgacgccca
      301 gctcgaccga gcggtcctcg agcgtgcctg cggcggtcag cagcctgacg gtggcgcgcc
      361 gcgacccgcg cgcggtctcg gtcgcgaatc cgctggtcga tgcggaggcc gggctctcgg
      421 ctgccgacgc agcgcggtcc ccgcgccgcg gctcgctggc ggagcgttca cgtcgccccg
      481 acgcatcgcc cgggcgcgtg ccgccgttca atgcggcagc ggggatcagc agcgcgtcct
      541 tcgccgtggc cgcgacgaag aacacctgtg ccgtcatgtt ggtcatcagc gactggttgg
      601 cgttcggtac atcgaacagc gcgttgtaga gcaccacgtt gttggtgacg gtcggcgtcg
      661 gctcgacctt gcgcagcgcg ccgtaccagc gccgaccctg gctgccgagg gtcgtgaagt
      721 agacctccat gccgcgtcgc aacttgctga catcggcttc cgacacctgt gtctgcaccg
      781 tcatcgtcga caggttggcg acgcgcagga tggtcggcgc ctgctggttc gcgttcaggg
      841 tctgcccctg ccgggcggtg accgtgacca ccgtgcctgc cagcggagcg tagatgcggg
      901 cgtagttcag gttggcctcg tctgcccgca gcgtcgattc ggtctgctcg atggacgcgc
      961 gcgtcgcatc gacctgggcc tccgcggctt gcgcattggc ttcggcctgc tgcagcgatt
     1021 cggtggtggt ggcgtcctcc ttcatcaggt tgcgctgacg cgtgagcagc agttccgcca
     1081 gcacccgttg cgattcgcgc tccgccaggg tggcccgctg gttgcgcagg ccggcgcgtc
     1141 gcgcgtcgac ggtggccttc agcaccgtgg ggtcgatctc ggccagcaga tccccggcct
     1201 gcaccgtcga gcccacctcg acatgcagct tcttcagctg accggacacc tgggcgccca
     1261 cgtccacata gtcgagcggc tgcaccgtgc cggtggcagt gaccaggtcc tggatgtcgc
     1321 cacgctggac ggtggcgaac acgtactgct cattcagatc agggcggccg tggtacgcgt
     1381 gccaggccca ccatgcgccg gcgccgatcg ccagcagcac gatgccgatc cagaggggcc
     1441 gactgagcag gaggcgacgc cacaacgggc ggaggagggc gggtgtctcg ggcatgggat
     1501 cggaggttcg gggcagggcg cgcggcaccg agtgtcgcgc gtcggtggtt aagcaatgca
     1561 ggggcccgtg ttgccgttgt gtaaaggggc tgcttgccgg ccgtggcggg cgatcgatgt
     1621 ctgacgccga aagtcgatcc ggatttcacc ggccagtcgg gccgtccggc cacggcggcg
     1681 cttcaccgct cgcgcaccag ctccagcacc gcatcggcga agacgtcagg ggcctcctgc
     1741 ggcaggttgt gtccggcacc tggcgcaatg cggtgcgtgc gcggcccggt gaagtgtcgc
     1801 gcgtgatgcg cggcgtcacg aggtgcaaac aatccgtcgt cggcgccgtc gaagctgatc
     1861 gttggcgcgc gaatcgctgg ctgctgcgcc aggcgccgct cgatctcggc atacgccggg
     1921 tcgccggcca ccaggccgta gcgatgacga taggaatgga tcgcgacgtc gacgaagtcc
     1981 gggttgtcga atgccgccgc actgcgatcg aacgtggcgt cgtcgaacac ccaggtcggc
     2041 gaccactgtt gccagagcag gcggcacatg gcgcggcggt cctgcatcag cccggcgcgc
     2101 ccgcgttcgc tgtggaagta gtactggtac cagagtcggt gctcctgtgc agggggctcg
     2161 ggctgcatgg cctgcgagat gtgctggatg ttgtaggaat tgagcgtcag caacccggca
     2221 catcgctcgg gccacagtgc cgccacgaca cacgccgcgc ggccgcccca gtcgtagccc
     2281 gccagcacgg cacgcgacac gcccagcgca tccagcagcg ccagcaggtc ggcaccgagc
     2341 gctgcctgct caccggagcg cggcgtgtcg gccgaccgga agcgcgtcgg cccgaagcct
     2401 cgcaggtacg gaacgatgac gcgacagccc cgttcggcca gcctcggagc gacatcgcgg
     2461 taggcatgga cgtcgtacgg gaacccgtgc agcagcacca ccggcggacc ttcggcagga
     2521 ccgctctcgt gataagcgac agtcagcacg ccggcgtcga tctcgcgtgg gcgcttcatt
     2581 ccggtcatgg tggggcaggg cggatgccga agagtcgact gttgcgcgcc cgactctacc
     2641 gcccgaagag caggtcccgg gcgggtcagt gcggcttgcg gggcttgcga aacgacttcg
     2701 gcgtgtagac gttgccttgc cactcgccgt catcggcatc ggaggtgcct cggcccttgc
     2761 gcgcccggtc gatggcggct tgcgcttcgc gcgtggcagc ccggcgcgcg gccggtgcgc
     2821 gccagaggcg ctgtgagcgg ttgcgcagct cccagccgag cgcgcgcact gcgtgatcga
     2881 agcgccgctg acgcgccgcg ccgaggcaga gccgaacgag cagcaccatg cacacggcgg
     2941 cggtgatgcc ggagatccac tgttcgaggg agttcatcgt gcgaccggtt gagacacggc
     3001 aagagccgga ttcagatcgt gatcatcgcg gagatcgggg catgacgcct gcgccggctg
     3061 gcaagccaca tcgatgaggt ggcgaaagcc cgcgggttca accgcgcagg gtcaactggc
     3121 ggcacaagtc gtccagacgg cgggcatcct cgtcggtgag caggccggcg cgcagtcgaa
     3181 ccgcgcgctc gatgcggcgc agtcgcaagc gcagttcgcc ttccaccgcc tcgacttcgg
     3241 ccgcgtcggg agccgccacg gggtcgatgc tccacagccc gctggtcatc aatccaccct
     3301 gcagccgggc cagcaagtgt tcggtcagcg tcagcgcgtc cttcagcgcg gccgaagtcg
     3361 cctcgcggcg cagcgtcgcg gtggccgtgc tcgcgtcgtg catgacctcg gcgagcttcg
     3421 tcagccgctc ctgcagctcc tccgccgtgc gcttggccgt tgcggtcggt tgctggcgaa
     3481 ccacctgcgt gatcagaccg acccagcgca ggtcgtactc gccgggcacc acgcgcagtg
     3541 cgatgctggc gcgcgacagc ggcgctgccg gctcggacag tgtggcgagc gtcgactcgg
     3601 tcaccgcgag cagccggccg tgcatcgaca gctggtcgcc gcgcaggcaa tgcggatagc
     3661 ccgagccgtc catgcgctcg tggtgttcgg cgatcgcccg ggcgacctcg gcggggtagt
     3721 cggtcagctg ggcgatcagc agctgtccga cgtgcggatg cacgaccagt tgctggtagg
     3781 tcagcacatc gacggtgcgt tctgcgtcgg cctcgccgaa ctccggagcg atgtacatct
     3841 cgcccaggtc gtgcagcagg ccgctcagca tcgcgatgcg caaatcggtg ggcttgccgc
     3901 cctgagccag catcaatgcg ccgttgaggg ccatcgcctg tgtcgcgtgc tcgtaggccg
     3961 ccggacgact gcttcgcgag gccgtcaaca gcagctgcgc caccgggtgc accggcagct
     4021 tggctgcctc gcgcagcagg gtgtcggcgt gcgcatgaag cagggcggtc agcggggtgg
     4081 gctgcttcag cagcctctcg atggcttcga ccagcgtgtg tgcggtcacg ccgtccgcgg
     4141 ccttcaggca gctttcgagc ggctcgcgca gctggcggtc gagcagcttg cgctgcagcg
     4201 cctgcgacac cggcaggtca cgtgcccaca gcttggtgcc cgagatgtcg aagatgtcgc
     4261 gcgcggcgat gatgctctgc gtctggctgg catccaggat ggtggccagg gcgtgctgat
     4321 tggcgcagga gaaatcggct gcgatcgcgg cggtgttgga cttcatggcg gctccgaggg
     4381 cggactgggt gatctctacc cgctgtttcg tcgcaatccc ggcattcttg agtgccggcg
     4441 cggccggtgg ctacaggtcc ttggt
//
LOCUS       DAOOCX010000176         4343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_19077_length_4343_cov_6.972715, whole genome shotgun sequence.
ACCESSION   DAOOCX010000176 DAOOCX010000000
VERSION     DAOOCX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19077_length_4343_cov_6.972715"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(398..1630)
                     /locus_tag="PK399_11750"
     CDS             complement(398..1630)
                     /locus_tag="PK399_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003158470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HPW13834.1"
                     /translation="MAVDDTAPRAPRQGPIALADLFDAALKDLTPNPNPRAPAPMAVP
                     TPAASGDAFLFSGNRHESVPRRLFVDRRLTPLERNAWQVFRLMLNDDGVTTFPTYEQL
                     RPWLASMPCAGQASHETVARALTLLRLTRWLSLVRRRRDPKTGRILGNLYVLHDEPLT
                     LFEAMQLDADYLALVSQSLGHSAKAVQVVGLNTLQEIADDPMLSGRTLPSRLQVLAER
                     LADQGITATASYPQEDAIHDSEEGPASLLRNGERPSSESEAGPKPAPDGALRNPKQDR
                     TVRSSRIDEVRTTAQARALGDLQWPKRFAELKAEQQAGARMALQQVDAALRQAVLDEW
                     AARCGKHGIRNPAGYLFGIIQRAMRGEFNAWAKQTSPAAPTPSAARAPPPEPPRNVVP
                     PAVARQHIERLRDLLRKS"
     gene            complement(1634..2194)
                     /locus_tag="PK399_11755"
     CDS             complement(1634..2194)
                     /locus_tag="PK399_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HPW13835.1"
                     /translation="MSTSHPLNQAVIAQALYDLRNGQLRRCKAMGFGEAELDALKHPA
                     LISVLANASVSWCSVTVNREVLQRLLNQAQDVEKEIATVDRMLRLGASTEMVSRFYGL
                     THQEVALRREILGLPKRKGRHPVLDETQDTELWRQWKAVTSSRNVDLEDETSILDAAM
                     DLAEGMSLPLSVVWAAIRNWVDQGLD"
     gene            complement(2209..3882)
                     /locus_tag="PK399_11760"
     CDS             complement(2209..3882)
                     /locus_tag="PK399_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HPW13836.1"
                     /translation="MADMTSQDMAGKLLAAGFERSGPAATALSDPIADTPMVVTLDQL
                     RPYDHDPRMKRNPAYEEIKASIRERGLDAAPAITRRPGEDHYIIRNGGNTRLAILREL
                     WSETKDERFFRISCLFRPWPERGEIVALTGHLAENELRGGLTFIERALGVEKAREFYE
                     QESSAALSQSELARRLAADGFPVQRSHITRMADAVRYLLPAIPTVLYGGLGRHQVERL
                     SVMRTACKRTWEHYAKDRTLPLDFDSFFQEVLAQFDTQGDEFATQRVQDELIGQMSEL
                     LGIGYDVLALDLTESESRHRALVSDPTPPAAPPALPSPTAGTPPPPGVASSIAKPAAA
                     ASPPPASPPASEPTLREDTARETTTESPTAAPGDLLLEHIVSPAPTTERLQSIQRMVA
                     DQLGDALPPDFSASVLQSIPVQAGGLYPISDVWYIDAGLDTPDRLRIHIAQFAREIAG
                     EAALDECIEDRSDGIGFVCCAPAHAPSPLGRAVLALLITLTGQPTAEAGVDGAQLAAD
                     LPALLHGQGQHHGDCTRRLSDTALVKLFRLLRLARRLLDLEAGTTAPGT"
     gene            complement(3875..4144)
                     /locus_tag="PK399_11765"
     CDS             complement(3875..4144)
                     /locus_tag="PK399_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13837.1"
                     /translation="MANAHELARGHKRLRALIEFAVGEGWHVKRTPGGHLKFTKAGCA
                     AIYTSSTASDHRAELNARAQIRRAEREARMAPAGGRGDCEGCGHG"
     gene            complement(4128..>4343)
                     /locus_tag="PK399_11770"
     CDS             complement(4128..>4343)
                     /locus_tag="PK399_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPW13838.1"
                     /translation="AIEAYPRAATRGLPVHRVEHRQPPGRVAPASLDTMRALASELFP
                     QWQDRFAAMSGRPPRPLDAGRSHGERT"
BASE COUNT          621 a         1437 c         1535 g          750 t
ORIGIN      
        1 gggagccgcc agcatccgca tgcagcggaa ggcagtcagt caacaaaccg aaatgagccg
       61 gcccccggtt ctatgcgcgc tggcggcgca gcgcgcaacg gtcccttgga ggccggagca
      121 ttcacgttgg cgctccgtgc ctcggtcgtt gacggtatgc gtactgcacc gcacgtcgcg
      181 ctgccgttcg acacgatcct tcgccgcgca gccgtcccca gtggaccgtt ccgttgagcg
      241 ccatggaggt ctgcttcatg gtcttgcagg cgctctactc aggttttgcg cagcatgtcg
      301 cgccgtcgtc cggcgtttca ccacacagcc gtcccctggg gaccgtttct gccgaccgcc
      361 aaggaggtct gcttcatggc cttgccaggc cctgcgctca ggatttgcgc agcagatcgc
      421 gcaggcgctc gatgtgctgc cgggccaccg cgggcggcac gacgttgcgc ggcggctcgg
      481 gcggtggcgc tcgcgccgcc gatggtgttg gcgctgccgg gctggtctgc ttggcccacg
      541 cattgaactc gccgcgcatg gcgcgctgga tgatgccgaa caggtacccg gcggggttgc
      601 ggatgccatg cttgccgcac cgtgcggccc actcgtccag cacagcctgc cgcagggcag
      661 cgtccacttg ctgcaacgcc atcctggcgc cggcttgctg ttccgccttc agttccgcga
      721 aacgcttggg ccattgcagg tcgcccagcg cgcgcgcctg cgcggtagta cgtacttcat
      781 caatacgact actacgtact gtacggtcct gcttcggatt ccgaagagcg ccgtctggcg
      841 cgggtttcgg ccctgcttcg gattccgaag aagggcgttc gccattccga agaaggctgg
      901 cgggcccttc ttcggaatcg tggatcgcat cctcctgtgg ataacttgcg gtggcggtga
      961 tgccctggtc ggccaggcgc tcggcgagca cttgcagccg cgacggcaag gtgcgtccgg
     1021 acaacatcgg gtcgtccgcg atctcctgga gggtgttgag tcccaccacc tggacggcct
     1081 tggcggaatg ccccagcgac tggctgacca gggccaggta gtcggcgtcg agctgcatgg
     1141 cctcgaacag tgtcaggggt tcgtcgtgca ggacgtagag gtttccgagg atgcggccgg
     1201 tcttggggtc gcgccgtcgt cgcacgaggc tcagccagcg cgtcaggcgc agcagcgtca
     1261 gcgcccgcgc gacggtctcg tgggaggctt gtcccgcgca gggcatggac gccagccatg
     1321 ggcgcaactg ctcataggtc gggaacgtcg tgacgccgtc gtcgttgagc atcagccgga
     1381 acacctgcca ggcgttgcgc tccagcggtg tcaggcggcg gtcgacgaac agccgccgcg
     1441 gcacgctctc gtgccgattg ccactgaata ggaaggcgtc gccggacgcc gcaggcgtgg
     1501 gcactgccat gggcgctggc gcgcggggat ttggattggg cgtgaggtcc ttcagcgcag
     1561 cgtcgaacag gtcggcgagt gcgatggggc cttgacgcgg ggctcgtggg gcggtgtcgt
     1621 ccacggccat ggcctagtcc aatccctgat cgacccagtt cctgatcgcg gcccagacca
     1681 ccgagagcgg cagtgacatg ccctcggcca ggtccatggc cgcatcgaga atcgaggtct
     1741 catcctccag atcgacgttc ctgctgctgg tcacggcttt ccattgccgc caaagttcgg
     1801 tgtcctgggt ctcgtccagg acggggtggc gccccttgcg cttgggcagg ccgaggatct
     1861 cccgccgcag ggcgacttcc tgatgggtca ggccatagaa gcggctgacc atctccgtgc
     1921 tggcccccag ccgcagcatg cgatcgaccg tggcgatttc cttctccacg tcctgtgcct
     1981 ggttgagcag gcgctgcagc acttcgcggt tgaccgtgac cgagcaccac gagacgctgg
     2041 cgttggccag cacgctgatc agcgcggggt gcttgagcgc atccagctcg gcctcgccga
     2101 agcccatggc cttgcagcgg cgcagttggc cgttgcgaag gtcatacagc gcctgggcaa
     2161 tcacggcctg gttgagcgga tgcgatgtgg acatgcggcc tccctcgctc acgtcccggg
     2221 cgccgtggtg ccggcttcca ggtccagcag acgccgggcc aggcggagca gccgaaatag
     2281 cttgaccaac gccgtgtcgc tcaagcgcct tgtgcagtcg ccgtggtgtt gaccctggcc
     2341 gtgcagcagt gctggcaagt cggcggcgag ctgcgcgcca tccacgcccg cttcagcagt
     2401 gggctgaccc gtcagagtga tgagcagcgc cagcaccgcc cggccgagcg gcgatggtgc
     2461 atgggcgggc gcgcagcaaa cgaagccgat gccatcggag cgatcctcga tgcactcgtc
     2521 cagggctgcc tccccggcga tctcgcgcgc gaactgcgcg atgtggatgc gcagccgatc
     2581 gggcgtgtcc aggccggcgt cgatgtacca gacgtcggag atcggataga gcccgccggc
     2641 ttgcaccggg atggactgca gcacgctcgc cgagaagtcg ggcggcagcg catcgcccaa
     2701 ctggtcggca accatgcgct ggatcgactg cagccgttcg gtcgtcggtg ccggcgagac
     2761 gatgtgctcg agaagcagat cgcctggtgc ggccgtgggg ctttccgttg tcgtctctcg
     2821 ggccgtatcc tcgcgcaagg tgggctcgga cgccggaggg ctggcgggtg gaggactggc
     2881 cgccgctgca ggcttggcga tggacgatgc tacgcccggg ggaggcggcg tgccggcggt
     2941 gggcgaaggc aacgccggcg gcgccgcagg tggcgtcggg tcactgacca gcgcccggtg
     3001 gcggctttcc gattcggtca ggtccagcgc caacacgtcg tagccgatgc ccagcagctc
     3061 ggacatctgg ccgatcagct cgtcctgcac gcgctgcgtc gcgaactcgt cgccctgcgt
     3121 gtcgaactgc gccagcacct cctgaaagaa gctgtcgaag tccagcggca gcgtgcggtc
     3181 cttggcatag tgctcccagg tgcgcttgca ggccgtccgc atgaccgaca gccgctcgac
     3241 ctggtggcgc cccaggccgc catagagcac agtgggaatg gcgggcagca ggtagcgcac
     3301 cgcgtcggcc atgcgggtga tgtgcgaccg ctggacgggg aacccatcgg ccgccaggcg
     3361 gcgggccagt tccgactggc tcagggcggc gctactctcc tgctcgtaga actcgcgtgc
     3421 tttctcgacg ccgagcgcgc gctcgatgaa cgtgaggcca ccgcgcagtt cgttctcggc
     3481 aagatgcccg gtcagcgcga cgatttcacc gcgctccggc catggccgaa acaggcacga
     3541 tatgcggaag aagcgttcgt ccttggtctc cgaccagagt tcgcgcagga tcgccagccg
     3601 cgtgttgccg ccgttgcgga tgatgtagtg gtcctcgccg ggccggcggg tgatggctgg
     3661 cgccgcgtcc aggccgcgct cgcggatgga cgccttgatt tcctcgtagg cggggttgcg
     3721 cttcatgcgc gggtcatggt cgtagggccg cagctggtcc agcgtcacga ccatgggcgt
     3781 gtccgcgatc gggtcgctca aggccgtagc cgctgggccg ctgcgctcga agccggccgc
     3841 gagcagcttg ccggccatgt cctgcgaggt catgtcagcc atggccgcac ccctcgcagt
     3901 ccccacgacc gccggccggc gccatgcggg cctctcgctc ggcgcggcgg atctgcgcac
     3961 gggcgttcaa ttcggcccgg tggtcgctcg cggtcgaact ggtgtagatc gccgcgcagc
     4021 cggccttggt gaacttgagg tgcccgcccg gcgtgcgctt gacgtgccag ccttcaccga
     4081 ccgcgaactc gatcagcgcg cgcagccgct tgtggccgcg ggccagttca tgtgcgttcg
     4141 ccatgggacc tcccggcatc aagaggacgt ggcgggcgac cggacattgc ggcaaatctg
     4201 tcctgccatt gcgggaacag ctcgcttgcg agcgcgcgca tcgtgtcgag cgaggccggc
     4261 gcgaccctgc cgggcggctg gcggtgctcg acccgatgca ccggcaggcc gcgcgtcgcg
     4321 gcgcgcggat aggcttcgat ggc
//
LOCUS       DAOOCX010000177         4324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_19196_length_4324_cov_7.535254, whole genome shotgun sequence.
ACCESSION   DAOOCX010000177 DAOOCX010000000
VERSION     DAOOCX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19196_length_4324_cov_7.535254"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..376
                     /gene="thiS"
                     /locus_tag="PK399_11775"
     CDS             176..376
                     /gene="thiS"
                     /locus_tag="PK399_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPW13839.1"
                     /translation="MDIQLNGEPRQIAPRATLAALLEHEGLAQRRVAVEVNGEIVPRG
                     RHATHVLQQGDIIEIVHALGGG"
     gene            533..1327
                     /locus_tag="PK399_11780"
     CDS             533..1327
                     /locus_tag="PK399_11780"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HPW13840.1"
                     /translation="MTTPLAHDPLVIAGKPYASRLLTGTGKFKDLDQTRLATEAAGAQ
                     IVTVAIRRTNIGQTPGEPNLLDVLPPDRYTILPNTAGCYTADDAVRTCRLARELLDGH
                     NLTKLEVLGDQRTLYPDVVQTLKAAEILVKDGFEVMVYTSDDPLLCKRLEEIGCVAVM
                     PLAAPIGSGLGIQNKYNLIEIIESAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAI
                     AGAKDPVLMAHAMKLAVEAGRAAFKAGRIPRRRFASASSPIDGLIG"
     gene            1464..2216
                     /gene="trmB"
                     /locus_tag="PK399_11785"
     CDS             1464..2216
                     /gene="trmB"
                     /locus_tag="PK399_11785"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPW13841.1"
                     /translation="MTDPGSKTSRPSSDGAKAPAKPFTITDGQRKVRSFVLRQGRFTE
                     AQERAFDTLWPRFGLDYAGTPRDFDAAFGRKAKRILEIGFGNGEAFRFSAQRDPARDH
                     IGIEVHAPGVGRLLNALATDDAGNARLYHHDAVEVLEHEVADGSLDEIRIYFPDPWHK
                     KRHNKRRLVNPAFAALLVRKLAPDGRLHLATDWQDYAEQMWDVLDASDGIANRAGARG
                     SVPRPDWRPQTHFETRGQKLGHGVWDLLYDRC"
     gene            2256..4094
                     /locus_tag="PK399_11790"
     CDS             2256..4094
                     /locus_tag="PK399_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPW13842.1"
                     /translation="MDTALTLTTDMELVLGLVVFTMVMFLFERIRADVVALVVLVVLG
                     LTGLVPREDLFGGFSGSAVMSIIATMILGAGLDRTGALNRLASWLLRRAHGVESRLLL
                     YTTAAAGFTSSIMQNPSVMSLFLPVASRLSSRTGLTLSRMLLPLAAAIVMGGALTMVG
                     NSPLILLNDLLVAANANLPSGAASLEPLNMFAPMPIGIALLAASLAYFHFFGSKLLRE
                     DGGDAVTPARTQSYFARAYGIDGDVHELTVTVDSPLVGMSLGDAESLHGAPLFLALQT
                     GNESRLSPPADARIWVGSVLGAMGPKQQVTDFAQNNFLRISSRLRAFGDLFNPSRAGI
                     SEAVVPPTSGFIGKTQAQLRLRKQHGLSLLAINRDKQVLREDVRNTAIRAGDMLVLHS
                     IWTDLAQAAKGKDFVVVTDYPKDEQRPHKFKVAMAIFAASMLLALSSKIPVPIALMTG
                     VAGMLVAGVIHVDEAYAAISWKTVFLMACLIPLGWAMDSSGAAAWLAGHTLEQLPEGF
                     PLWLLQLLIGLLTTVFSLAISHVGATIIMVPIAINVALAAGGNPTAFALIVALSASNN
                     LMTASNPVMSMVTGPANYTGRDLWRVGGPLSLAYLVVIVIAINLLY"
     gene            complement(4126..>4324)
                     /locus_tag="PK399_11795"
     CDS             complement(4126..>4324)
                     /locus_tag="PK399_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HPW13843.1"
                     /translation="PHAGRRLDWAPGKFLRAKDGMLVCAAHGASFELANGVCVAGPCR
                     GESLRAVAVEVRDGGISLAQG"
BASE COUNT          706 a         1466 c         1461 g          691 t
ORIGIN      
        1 agggtggctt tcatggggag tcctcggtgg atcgggtggg atgcgccgga gtgcccgcca
       61 atggacggag gcgccgacat acgacagcga atggcgggta atggtttcac gaaaatctaa
      121 cggcttcatg ttgcaccgcc gcattccgtg cgacggaacc ggcgacaatc gcggcatgga
      181 catccaactc aatggcgagc cccgccagat cgcgccacgg gccaccctcg ccgcgctgct
      241 cgaacacgaa ggcctggccc agcgccgggt cgcggtggag gtcaatggcg agatcgtccc
      301 gcgcggccgc cacgccacgc atgtcctgca gcagggcgac atcatcgaga tcgtgcatgc
      361 gctcggtggt gggtgaggcc tggcgtttgc gcggcactgc cgcgcgccaa gccgaacgcc
      421 cggccatgga tggccgggca ggacattccg aagcggagaa ggcagcgcca tggatggcga
      481 cgcaatgcct gtcgaagcgg catcgcagtg cgccatcggc gataattccc cgatgacgac
      541 tcccctcgcg cacgacccgc tggtcatcgc cggcaaaccg tatgcctcgc gcctgttgac
      601 cggcaccggc aagttcaagg atctcgacca aacccgcctg gccaccgagg ccgccggcgc
      661 gcagatcgtc accgtggcga tccgccgcac gaacatcggc cagaccccgg gcgagcccaa
      721 cctgctcgac gtgctgcccc cggaccgcta caccatcctg cccaacaccg ccggctgcta
      781 caccgccgac gatgcggtgc gcacctgccg gctggcgcgc gaactgctgg atggccacaa
      841 cctgaccaag ctggaagtgc tgggcgacca gcgcacgctg tatcccgacg tcgtgcagac
      901 cctcaaggcc gccgagatcc tggtcaagga cggcttcgag gtgatggtgt acaccagcga
      961 cgatccactg ctgtgcaagc ggctggagga gatcggctgc gtggcggtga tgccgctggc
     1021 ggcgccgatc ggctccggcc tgggcatcca gaacaagtac aacctgatcg agatcatcga
     1081 gagcgcgaag gtgccgatca tcgtcgatgc cggcgtgggc actgcgtcag acgcggcgat
     1141 cgcgatggag ctcggctgcg atggcgtgct gatgaacacc gcgatcgccg gggcgaagga
     1201 tccggtgctg atggcgcatg cgatgaagct cgcggtcgag gccggccgcg ccgcgttcaa
     1261 ggccgggcgc atcccgcgca ggcgcttcgc gtcggcgtcg tcgccaatcg atggattgat
     1321 tgggtgagcc cgtgcgcttg cgagccactg gctcgcgcac cggtgaacgc ccggccacgg
     1381 atggcccggc aggcgttcgg tttactacgg tgttgcgcca tggatggcga tgacgctgtt
     1441 cgcctgtcga tgaggtgaac gagatgaccg atccaggctc caaaaccagc agaccttcga
     1501 gcgatggcgc gaaggcgccc gccaagccct tcaccatcac cgacggccaa cgcaaggtgc
     1561 gcagcttcgt gctgcgccag ggccgcttca ccgaggcgca ggagcgcgca ttcgatacgc
     1621 tgtggccgcg cttcgggctg gattacgcag gcacgccgcg cgatttcgac gccgcgttcg
     1681 ggcgcaaggc gaagcgcatc ctcgagatcg gcttcggcaa tggcgaggcc ttccgcttct
     1741 ccgcgcagcg cgacccggcg cgcgaccaca tcggcatcga agtgcatgcg ccgggcgtcg
     1801 gccgcttgtt gaacgcattg gccacggacg atgccggcaa cgccaggctc taccaccacg
     1861 atgcggtgga agtgctggaa cacgaagtcg ccgatggatc gctggacgag atccgcatct
     1921 acttcccgga tccctggcac aagaagcgcc acaacaagcg gcggctggtg aatcccgcgt
     1981 tcgccgcgtt gctggtgcgc aagctggcgc cggatggccg cttgcacctc gccacggatt
     2041 ggcaggacta tgcggaacag atgtgggacg tgctggatgc gagcgacggc atcgccaatc
     2101 gcgccggcgc gcgcggttcg gtaccgcgcc ccgactggcg gccgcagacc cacttcgaga
     2161 cccgcggcca gaagctgggc cacggcgtct gggacctgtt gtacgaccgc tgctgatccg
     2221 ccgatcgaac acggacaggg acgccacgcc gccacatgga caccgcgctg acgctcacca
     2281 ccgacatgga gctcgtgctc gggctggtgg tcttcacgat ggtgatgttc ctgttcgagc
     2341 gcatccgtgc cgacgtggtc gcgctggtcg tgttggtggt gctgggcctg accgggctgg
     2401 tgccgcgcga ggacctgttc ggcggcttct ccggcagcgc ggtgatgagc atcatcgcga
     2461 cgatgatcct gggcgcgggg ctggatcgca ccggcgcgct caaccggttg gccagctggt
     2521 tgttgcggcg cgcgcatggc gtggaatcgc ggctgctgct ctacaccacc gcggccgcag
     2581 gcttcaccag ttcgatcatg cagaacccgt cggtcatgtc gctgttcctg ccggtggcgt
     2641 cgcggctgtc gtcccgcacc ggcctgaccc tgtcgcggat gctgctgccg ctggcggcgg
     2701 cgatcgtgat gggcggcgcg ttgaccatgg tcggcaattc gccgctgatc ctgctcaacg
     2761 acctgctggt cgcggccaac gccaacctgc cctccggcgc ggcctcgctg gaaccgctca
     2821 acatgttcgc accgatgccg atcggcatcg ccctgctggc ggcatcgctg gcgtatttcc
     2881 atttcttcgg cagcaagctg ctgcgcgagg acggcggcga tgcggttacc ccggcgcgca
     2941 cccaaagcta cttcgcccgc gcctacggca tcgacggcga tgtgcacgag ctgaccgtga
     3001 cggtggacag cccactggtc ggaatgagcc tgggcgatgc cgagtcgctg cacggcgcgc
     3061 cgctgttcct ggccttgcag accggcaacg agtcccgcct gtcgccaccg gccgatgcgc
     3121 ggatctgggt cggcagcgtg ctcggcgcga tggggccgaa gcagcaggtc accgacttcg
     3181 cgcagaacaa cttcctgcgg atctcgtcgc ggctgcgtgc gttcggcgac ctgttcaatc
     3241 ccagccgcgc cggcatttcc gaagcggtgg tgccgccgac ttccggcttc atcggcaaga
     3301 cgcaggcgca gctgcgcctg cgcaagcagc acgggctcag cctgctggcg atcaaccgcg
     3361 acaagcaggt gctgcgcgag gacgtgcgca acaccgcgat ccgcgccggc gacatgctgg
     3421 tcctgcacag catctggacc gacctggcgc aggcggcgaa gggcaaggac ttcgtggtgg
     3481 tcaccgacta cccgaaggac gagcagcgcc cgcacaagtt caaggtcgcg atggcgatct
     3541 tcgcggcatc gatgctgctg gcgctgtcct cgaagatccc ggtgccgatc gcgctgatga
     3601 ccggcgtggc cggcatgctg gtggcaggcg tgatccacgt cgacgaggcc tacgcggcga
     3661 tcagctggaa gaccgtgttc ctgatggcct gcctgatccc gcttggctgg gcgatggaca
     3721 gcagcggcgc cgccgcgtgg ctggcgggac acacgctgga gcagttgccg gaaggcttcc
     3781 cgttgtggct gctgcaactg ctgatcggcc tgctgaccac ggtgttctcg ctggcgatca
     3841 gccacgtcgg cgccacgatc atcatggtgc cgatcgcaat caacgtggcg ctggcggccg
     3901 gcggcaaccc cacggcattc gcgctgatcg tggcgctgtc ggcctccaac aacctgatga
     3961 ccgcgtccaa tcccgtcatg tcgatggtca ccggtcctgc caactacacc ggccgcgacc
     4021 tgtggcgcgt cggcgggccg ctgtcgctgg cctacctggt cgtgatcgtc atcgcgatca
     4081 acctgctcta ctgaccaggg tcgttgcgca agcccagaac tcgactcagc cttgcgcgag
     4141 cgaaatcccg ccatcgcgca cttcgaccgc aacggcacgc agcgactcgc cccgacaggg
     4201 gccggccacg cacacgccgt tggccagttc gaagctggcg ccatgcgcgg cgcagaccag
     4261 catcccgtcc ttggcgcgca ggaacttgcc cggcgcccaa tccagccggc gtcccgcatg
     4321 cggg
//
LOCUS       DAOOCX010000178         4310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_19276_length_4310_cov_5.940306, whole genome shotgun sequence.
ACCESSION   DAOOCX010000178 DAOOCX010000000
VERSION     DAOOCX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19276_length_4310_cov_5.940306"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PK399_11800"
     CDS             <1..808
                     /locus_tag="PK399_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPW13844.1"
                     /translation="PEADIHLERLDMGVSASPDVVVLDPVDPKRVSGGSPLELDLALV
                     MGDAVKIDGFGLAGTLGGSLRVLQSPGRDMRGTGALEVAGRYRAYGQNLQITRGRLVW
                     SNTAIGDPLLDLRAEREVGDVTAGIRVEGRASAPRATVYSNPAKSESEALAYLTLGRP
                     LSSLTGAEAGQLGAAKSALNAGTGLLAAELGARIGLDDAGVTESRALGADVLTVGKYL
                     SPKLYVGYGVSLLGTGQVLMLKYLLRKGFDIQIESSTVENRASVNWRKEK"
     gene            complement(882..3734)
                     /locus_tag="PK399_11805"
     CDS             complement(882..3734)
                     /locus_tag="PK399_11805"
                     /inference="COORDINATES: protein motif:HMM:NF017476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13845.1"
                     /translation="MKSTTFHCRRAPAFSLLTLALLGAMAMPAWAADPECVDANGNPV
                     PGNTDQANEGGTDNATCNVAASAYGYANNASGVQSNAFGAFNTASSGFAGAFGYANNA
                     FGLLGSAFGVYNVASGENSSAFGYANTASALSSVAMGNSSVASNLNSAAVGTWYDDDF
                     NGEFDAGADVVNTATGQYSGAFGVGNTASGYFSQAFGVLNTASGGASVAMGFGNDAVG
                     ANTTAVGLGNTANGNKSSAFGYQSVANGVGSVALSGWIDLDGDGQFDADEAATAGGVS
                     SLAVGTGAAWGTSSSAVGVDNTVTGSRGSAFGYGNHAWEGSSAFGFGNTASDLNNSAF
                     GYGNSTASEYTSAFGYRNTATSQYSSAFGYNGHARGQGSVVVSGWYDRDNNGILTLNL
                     DLDGDGINESSSETSRASGVGAVALGTGAQATAIRSSAVGVGNTASEDRSNAYGFANT
                     ASGFASNALGFTNTASGYGSSAIGFLNTTEGVYDSGVGYRNLASGGSSSAFGNNNTAA
                     GLYSSAFGYSNKASVGGSTALGYGNTASGVNSSAFGSLSTALNIGSTAIGFQAVADRD
                     YAVAVGRAGGEHQIIHLADGTEDYDAVNVRQLRSLVNWFGGGASFTGGVFNAPSFTIQ
                     GTSYGNVAAAFAAVDTRLTQLAANVGGPQGPAGPAGPQGPTGPTGPQGPEGPSGPAGP
                     TGPTGPEGDQGPAGPAGPQGEPGDQGPAGPQGDQGPTGPQGPAGQDGEDADMTEVAQM
                     VAAGDAATLDAAQQYADAGDAATLEQANAHADAGDAATLGQANTHADAGDASTLTSAN
                     AYADAGDTATLAAAKAHATAGDAATLTTSRAYTDTTATQTLASAKTYTDQKFAAWTDT
                     FTQYQQQVDTRFAQTDRRIDQIGAMGSAMTQMAVNAANGSSEKGRIAIGVGAQGSQGA
                     VSIGYGKRLGDRASFSFGASFSSGESSAGAGFGFDL"
     gene            3876..>4310
                     /locus_tag="PK399_11810"
     CDS             3876..>4310
                     /locus_tag="PK399_11810"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HPW13846.1"
                     /translation="MSDPSVTQWLHRARDGEAEALGRAYAAAYDDLKQTAHAQLRRGG
                     SGLDTTSLVNETYLKLARSDGFQPEDRKALMALSAHAMRQVLVDQARRQQADKRGGGA
                     AAVTLHTGLSDSVPALDVLELNDLLSSLAQADPRAAEGVELRC"
BASE COUNT          630 a         1466 c         1454 g          760 t
ORIGIN      
        1 gccggaagcc gacatccacc tggagcgcct ggacatgggc gtgtccgcat cgccggacgt
       61 ggtggtgctg gacccggtcg atcccaagcg cgtctccggc ggcagcccgc tcgaactcga
      121 cctggcgctg gtgatgggcg atgcggtgaa gatcgacggc ttcggcctgg ccggcacgct
      181 cggcggcagc ctgcgcgtgc tgcagtcgcc cggacgtgac atgcgcggca ccggcgcact
      241 ggaagttgcc ggccgctatc gcgcgtatgg ccagaacctg cagatcaccc gtggccggtt
      301 ggtgtggtcg aacaccgcga tcggcgatcc gctgctcgac ctgcgtgccg aacgcgaagt
      361 gggcgacgtc accgccggca tccgcgtcga gggccgtgcc tccgcaccgc gcgccaccgt
      421 ctactcgaac cccgcgaaga gcgaatccga agcgttggcc tacctgacat tgggtcgccc
      481 gttgtcgagc ctgaccggcg ccgaagccgg ccagctgggc gcggcgaaat ccgcgctcaa
      541 cgcaggcacc ggtctgctcg ctgcggagct gggcgcacgc atcggcctgg acgatgccgg
      601 cgtgaccgaa tcgcgcgcgc tcggcgccga cgtgctgacc gtgggcaagt acctgtcgcc
      661 gaagctgtac gtcggctatg gcgtctccct gctcggcacc ggccaggtgc tgatgctgaa
      721 gtacctgctg cgcaagggct tcgacatcca gatcgaatcg agcacggtgg agaaccgcgc
      781 gtcggtgaac tggcgcaagg aaaagtaatt cgtcgatgcc agcgcaaacg aaaacggcgc
      841 ggttgctcgc gccgttccgc attgcatgtc gcttgacgca attacaggtc gaagccgaag
      901 cccgcgccgg ccgaggattc gccgctgctg aagctcgcgc cgaacgagaa cgaagcgcgg
      961 tcgccgaggc gcttgccgta accgatcgac accgcgccct ggctgccctg cgcgcccacg
     1021 ccgatcgcga tgcggccctt ctcgctgctg ccgttcgccg cgttcaccgc catttgcgtc
     1081 atcgcgctgc ccatcgcgcc gatctggtcg atgcggcgat cagtctgggc gaagcgcgtg
     1141 tccacctgct gctggtactg ggtgaaggta tcggtccagg ccgcgaattt ctggtcggtg
     1201 taggtcttcg ccgaagccag cgtctgcgtg gcagtggtgt cggtgtaggc cctcgacgtg
     1261 gtcagggtcg cggcatcgcc ggcggtggcg tgcgccttgg cggcggccag cgttgcggtg
     1321 tcgcctgcgt ccgcataggc gttggcggat gtcagcgtag aagcatcacc ggcatcggca
     1381 tgggtgttgg cctgtccgag cgttgccgcg tcgccggcat ccgcatgcgc gttcgcttgc
     1441 tccagcgttg cggcatcgcc tgcatcggca tattgctgcg ctgcgtccag cgtcgcggca
     1501 tcgccggctg cgaccatctg cgccacctcg gtcatgtcgg catcctcgcc atcctgtccg
     1561 gccgggcctt gtggaccggt cgggccttgg tcgccctgcg gacccgcggg gccctgatcg
     1621 cccggctcgc cttgcggacc agcgggaccg gccgggccct ggtcgccttc tggaccggtg
     1681 gggcccgtcg ggccagcggg acctgacgga ccctcggggc cttgtggccc ggtcgggccg
     1741 gtggggcctt gtgggcctgc cggaccggcc ggaccttgcg gcccaccgac attggcggcc
     1801 agttgggtca gcctggtgtc caccgccgcg aacgcggccg ccacgttgcc gtagctggtg
     1861 ccctgaatgg tgaagctcgg cgcgttgaac acgccgccgg tgaagctggc gccgccaccg
     1921 aaccagttca ccagcgaacg caactggcgc acgttgaccg cgtcgtagtc ctcggtgcca
     1981 tcggcgaggt ggatgatctg gtgctcgccg ccggccctgc ccacggcgac ggcgtagtcg
     2041 cggtccgcga cggcctggaa gccgatcgcc gtgctgccga tattcaaggc cgtggacagg
     2101 gatccgaatg cactgctgtt gacgcccgat gccgtgttgc cgtagcccaa cgccgtgctg
     2161 ccgccgacgc tggccttgtt gctgtagccg aacgcgctgc tgtaaagccc cgctgcggtg
     2221 ttgttgttgc cgaacgcgct gctggatccg cccgacgcca agttgcgata gccgaccccg
     2281 ctgtcgtaga cgccttccgt cgtattgagg aagccgatag cgctgctgcc gtagccactc
     2341 gccgtgttcg tgaagcctaa ggcgttgctg gcgaaaccgc tggcggtgtt ggcgaagcca
     2401 taggcgttgc tgcggtcctc gctcgcggtg ttgcccacgc caaccgcgct gctgcggatg
     2461 gcggtcgcct gcgcgcccgt ccccagcgca actgctccaa cgcccgaggc gcgcgacgtt
     2521 tcgctggagg actcgttgat gccatcgcca tccaggtcga ggttcagggt caagatgccg
     2581 ttgttgtcgc ggtcgtacca accggagacg acaacgctgc cttggcccct tgcgtggccg
     2641 ttgtagccga aggcgctgct gtactggctg gtggcggtat tccggtagcc gaaggcgctg
     2701 gtgtattcgc tggcggtgct atttccgtac ccgaaggcac tgttgttcag gtcgcttgcg
     2761 gtgttgccga agccgaaggc actgctcccc tcccaggcat gattcccgta tccgaaggcg
     2821 ctgcccctgg agccggtcac cgtgttgtcc acccccactg cgctgctaga cgttccccat
     2881 gcggcgccgg tgccgaccgc cagcgaggaa actcctcccg ccgtcgccgc ctcgtccgca
     2941 tcgaactgac cgtcgccatc cagatcgatc caacccgaca aggcgacgct gcccacgcca
     3001 ttcgctacgc tctggtaacc gaatgcgctg ctcttgttgc cgttcgcggt gttgcccagt
     3061 ccaacggcgg tggtgttggc accaacagcg tcgttgccga accccatcgc cacgctggcg
     3121 ccaccgcttg cggtgttgag cacgccgaac gcctggctga agtagccgct ggcggtgttg
     3181 cccacgccga aagcgccgct gtactgccca gttgcggtgt tcactacgtc cgcgccggca
     3241 tcgaattcac cattgaaatc gtcgtcatac caggtcccca cggccgcact gttgaggttc
     3301 gaggcaaccg agctattgcc catggcgacg ctggaaagcg cgctggccgt gttggcgtaa
     3361 ccgaaggcgc tgctgttttc gccactggcc acgttgtaga cgccgaaggc gctgccaagc
     3421 aggccgaacg cgttattcgc atagccgaat gctccggcga agccggaact ggcggtgttg
     3481 aaggcgccga aggcgttgct ttgcacgcca ctggcgttgt tcgcatagcc ataggccgat
     3541 gccgcgacgt tgcaagtcgc gttatcggta ccaccctcgt tggcctggtc ggtgttaccc
     3601 ggtaccgggt tgccgttggc atcgacgcat tccggatccg cggcccaggc cggcattgcc
     3661 atcgcgccca gcagggccag ggtcaggagg gaaaaagccg gcgcacgccg gcagtggaag
     3721 gtggtggact tcatcgtggt tcccctttcg gtcttcgaat ccgcgcgaaa tgcgcgtcta
     3781 tccctccatg cgcagaaagc cggtgaatcc cgacatggcc ccggaaatcg ccaccgctcc
     3841 ggctatgctt gggcggcccc accgccggac tgctcatgag cgatccctcc gtcacccagt
     3901 ggttgcaccg cgcccgcgac ggcgaggccg aggccttggg tcgcgcctac gctgccgcct
     3961 acgacgacct caagcagacc gcgcacgcgc agctgcgacg cggcggcagc ggactggaca
     4021 ccacctcgct ggtcaacgag acctacctca agctggcccg cagcgacggc ttccagccgg
     4081 aggaccgcaa ggcgctgatg gcgctgtccg cgcatgcgat gcggcaggtg ctggtcgacc
     4141 aggcgcgccg gcagcaggcc gacaagcggg gtggtggtgc ggcggcagtg accctgcata
     4201 ccgggctttc tgacagcgtg ccggcgctgg acgtgctgga actcaacgac ctgctgtcgt
     4261 cgctggcgca agccgacccg cgcgccgccg aaggcgtgga actgcgctgc
//
LOCUS       DAOOCX010000179         4242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_19701_length_4242_cov_6.235968, whole genome shotgun sequence.
ACCESSION   DAOOCX010000179 DAOOCX010000000
VERSION     DAOOCX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19701_length_4242_cov_6.235968"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK399_11815"
     CDS             <1..212
                     /locus_tag="PK399_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13847.1"
                     /translation="GLGVHKRQAVYEMLVMSQRLRELVVPGAEADTIHRVAVEEGMRP
                     ITQAAVALARTGAISLAEVWRVRAD"
     gene            complement(342..725)
                     /locus_tag="PK399_11820"
     CDS             complement(342..725)
                     /locus_tag="PK399_11820"
                     /inference="COORDINATES: protein motif:HMM:NF018697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW13848.1"
                     /translation="MKMQERIRRARRRAGLSQTGLAALVEVRRSAVSNWESADGVLPS
                     MQNLLAIAKACRVSLEWLGTGRGGISTDPDGLADVPAADAELVDVEEERTLLAGFREL
                     PRRSQQLVLDLVDTLHSSRRHTGRG"
     gene            complement(1051..2004)
                     /gene="ispH"
                     /locus_tag="PK399_11825"
                     /gene_synonym="lytB"
     CDS             complement(1051..2004)
                     /gene="ispH"
                     /locus_tag="PK399_11825"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPW13849.1"
                     /translation="MDILLANPRGFCAGVDRAIEIVKRAIETLGAPIYVRHEVVHNKF
                     VVDDLKARGAIFVEELDEVPDGNTVIFSAHGVSQAVRREADRRGLKVFDATCPLVTKV
                     HLEVARQCRNGRDMVLIGHAGHPEVEGTMGQWSREGGVGTIHLVEDIDDVATLAIAQP
                     ANCSFTTQTTLSVDDTRDIIAALKARFPQMQGPKNDDICYATQNRQDAVRELVGEGCD
                     AVLVVGSPNSSNSNRLRELAEREGADAWLIDGAEEIDPVWVAGRKRIGVTAGASAPDV
                     LVQGVLERLRELGAGSVRELHGEPEDMVFALPKELRLRLVD"
     gene            complement(2012..2512)
                     /gene="lspA"
                     /locus_tag="PK399_11830"
     CDS             complement(2012..2512)
                     /gene="lspA"
                     /locus_tag="PK399_11830"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPW13850.1"
                     /translation="MAVVKPNALPWLGASALVLLLDQLTKAWVLASLPEYTAVPVIDG
                     FWNWYRTYNTGAAFSFLANAGGWQKWFFTVLAFGISGLMAWMLARTPRRDWKQALPLA
                     LVIGGAIGNVIDRLQHGHVVDFIQWHVGEHYWPAFNIADCAVVGGAIGIALFGLLSGK
                     PKIKAG"
     gene            complement(2512..>4242)
                     /locus_tag="PK399_11835"
     CDS             complement(2512..>4242)
                     /locus_tag="PK399_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPW13851.1"
                     /translation="PVDGRGVYLPSTPPAHGTELAGLHVWKANDVLVEVLRASGHLLA
                     FSKLTHSYPHCWRHKTPVAFRATPQWFISMDQAHLRRDALAAIKTVTWYPEWGEARIA
                     GMIDGRPDWTISRQRTWGVPIALFVHRETGEPHPRSVELMRHVADRVEQGGVDVWYSL
                     DAADLLGDEANDYDRITDILDVWFDSGITHEAVLLERGLGKPADLYLEGSDQHRGWFQ
                     SSLLTGVAIDKAAPYRQCLTHGFTVDEHGRKMSKSLGNGIEPQDIMKTLGADILRLWI
                     ASADYSNEMSLSQEILKRNADAYRRIRNTARFLLGNLHGFEPARDLRPLEDMVALDRW
                     IVHRAWQVQEDIAAAYARYDFAAVVQSLMNFCSVDLGSLYLDVTKDRLYTMGEDSRGR
                     RSAQSAMFRITEAFVRWIAPVLSFTADEMWPQIPGERAGNVLFATWYDGLAPLPGDAA
                     LSMTDFDALLQVRDQVAKVLEPMRAAGEIGAALQAEIELRCGVSDQNWLSPLADELRF
                     LLISGDVRVVPDAEAKGAIGVFAQPTTKAKCVRCWHHRGDVGAHAAHPQLCGRCVSNI
                     EGPGEDRMWF"
BASE COUNT          684 a         1474 c         1385 g          699 t
ORIGIN      
        1 aaggcctggg cgtccacaag cgacaagccg tctacgaaat gctggtcatg agccagcgcc
       61 tgcgcgagct cgttgtcccc ggcgccgaag cggacaccat ccaccgcgtc gcggtggagg
      121 aaggcatgcg cccgatcacc caggctgcgg ttgcactcgc gcggaccggc gcgatttcgc
      181 tggcggaggt gtggcgagtc cgcgccgact gagcgctacc agggtcctgc gggacgctca
      241 ggccgggcca gaacgcattg cggatgcgcc accctgccaa ctcgtggtgg acgaccctgg
      301 ccgctgcgca gttccccacc ggtgcgggcg tgattgaccg gtcagccccg acccgtatgg
      361 cggcggctgg aatgcagggt gtcgaccagg tcgagtacca actgctgcga tcgccgcggc
      421 agctcgcgga agccggccag cagcgtgcgc tcttcctcga catccaccag ttccgcatcg
      481 gccgccggca cgtcggccaa tccgtccgga tcggtcgaga tgccgccacg gccggtgccc
      541 agccattcca gcgacacccg gcaagccttg gcgattgcca gcaggttctg catcgacggc
      601 aggaccccat cggcggattc ccagttggag accgcgctgc ggcgcacctc gaccaatgcg
      661 gccaacccgg tttgcgagag cccggcgcgc cggcgcgcgc ggcggatccg ttcctgcatc
      721 ttcatcgcgc ctccaatgcc agccatcatg gccgccatcg atattggcac aaggttgtac
      781 aggattgctg tctttcatgc actgaaagcg ctttgaatgg tcgctccagg cggtgccaga
      841 cagcgggaat ggcgtcgatc gctgatgcaa tcccgtgcat tcacaagcag catagcggct
      901 cgggcgacac ggacgttgcc cgctcctccc cccggggcgc cggagctcct cgtcatcgat
      961 gccacgcatt gattccgtgc tgccccgctt tatgcaaggt tgaacgccac gttcccagcg
     1021 tggcgttcct tttttccccg gatggcgcgc tcagtcgacc aggcgcaggc gcagttcctt
     1081 gggcagggcg aacaccatgt cctcgggttc accgtgcagc tcgcgcacgc tgccggcacc
     1141 gagttcacgg agtcgctcca gcacgccctg caccagcacg tccggtgcgg aagcgccggc
     1201 ggtgacgccg atgcgcttgc gccccgccac ccacacggga tcgatctcct cggcaccatc
     1261 gatcagccag gcgtccgcgc cctcccgttc ggccagttcg cgcaggcggt tcgaattgga
     1321 ggaatttggc gagccgacca ccagcacggc gtcgcagccc tcgccgacca actcgcggac
     1381 cgcatcctgg cgattctggg tggcgtagca gatgtcgtcg ttcttcggcc cctgcatctg
     1441 cgggaaacgc gccttcaatg ccgcgatgat gtcgcgcgtg tcgtccacgc tcaacgtggt
     1501 ctgggtggtg aaggagcaat tggcgggctg ggcgatggcg agcgtggcga catcgtcgat
     1561 gtcctcgacg aggtggatcg tccccacccc gccttcgcgg ctccattggc ccatcgtgcc
     1621 ctcgacttcc gggtgccccg cgtggccgat caggaccatg tcgcggccat tgcggcattg
     1681 ccgcgcgacc tccaggtgga ccttggtgac cagcgggcag gtcgcgtcga acaccttcag
     1741 gccgcggcga tcggcttcgc ggcgcaccgc ctgcgagacg ccatgcgcgc tgaagatcac
     1801 ggtgttgccg tccggcactt cgtccagttc ctcgacgaag atcgcgccgc gtgccttgag
     1861 gtcgtcgacc acgaacttgt tgtgcacgac ttcgtggcgc acgtagatcg gcgcgcccag
     1921 ggtctcgatg gcgcgcttga cgatctcgat ggcgcggtcg acgccggcgc agaagccacg
     1981 cgggttggcg agcaggatgt ccatcgccgg attatcccgc cttgatcttg ggcttgccgg
     2041 agagcagccc gaacaaggcg atgccgatcg cgccgccgac caccgcgcag tcggcgatgt
     2101 tgaacgccgg ccagtagtgc tcgccgacat gccactggat gaagtcgacc acgtgcccat
     2161 gttgcaggcg gtcgatgaca ttgccgatcg cgccgccgat caccagcgcc agcggcagcg
     2221 cctgtttcca gtcgcggcgc ggcgtgcgcg cgagcatcca tgccatcaat ccgctgatgc
     2281 cgaaggcgag cacggtgaag aaccatttct gccagccgcc ggcgttcgcc aggaaactga
     2341 aggccgcgcc ggtgttgtag gtgcggtacc agttccagaa cccgtcgatc accgggaccg
     2401 cagtgtattc cggcagcgac gccagcaccc aggccttggt cagctggtcc agcagcagga
     2461 ccagcgcgga ggcacccagc cagggcagtg cgttcggctt caccacggcc atcagaacca
     2521 catccggtct tcgcccggcc cttcgatgtt ggagacgcaa cgcccgcaca gctgcggatg
     2581 cgcggcatgc gcgccgacgt cgccgcgatg gtgccagcag cgcacgcact tggccttggt
     2641 cgtcggctgc gcgaacacgc cgatcgcgcc cttcgcctcg gcatccggca ccacccgcac
     2701 gtcgccgctg atcagcagga agcgcagctc gtcggccagc ggcgacagcc agttctggtc
     2761 ggacacgccg cagcgcaatt cgatctccgc ctgcaacgcg gccccgatct caccggccgc
     2821 gcgcatcggt tccagcacct tggcgacctg gtcgcgcacc tgcagcaggg catcgaagtc
     2881 ggtcatcgac agggccgcgt cgcccggcag cggtgccaac ccgtcgtacc acgttgcgaa
     2941 cagcacgttg ccggcgcgct cgcctgggat ctgcggccac atctcgtcgg cggtgaagct
     3001 cagcaccggc gcgatccagc gcacgaaggc ctcggtgatg cggaacatcg ccgattgcgc
     3061 gctgcgacgg ccgcgcgagt cctcgcccat cgtgtacagg cggtccttgg tgacgtcgag
     3121 gtacagcgaa ccgaggtcca cgctgcagaa attcatcagc gactgcacca ccgcggcgaa
     3181 gtcgtagcgc gcgtacgccg cggcgatgtc ttcctgcacc tgccaggcgc gatgcacgat
     3241 ccagcgatcc agcgcgacca tgtcctccag tgggcgcagg tcgcgcgccg gctcgaagcc
     3301 atgcaggttg ccgagcagga agcgcgcggt gttgcggatg cgccggtagg cgtccgcgtt
     3361 gcgcttgagg atttcctgcg acagcgacat ctcgttgctg taatcggcag acgcgatcca
     3421 caggcgcagg atgtccgcgc ccagcgtctt catgatgtcc tgaggctcga tgccattgcc
     3481 cagcgacttc gacatcttgc ggccgtgctc gtccacggtg aagccgtggg tcaggcactg
     3541 ccgatacggc gcggccttgt cgatggcgac gccggtcagc agcgaactct ggaaccaccc
     3601 gcgatgctgg tcggagcctt ccaggtacag atccgcgggc ttgccgaggc cgcgctccag
     3661 cagcaccgct tcatgggtga tgccggaatc gaaccagaca tcgaggatgt cggtgatgcg
     3721 gtcgtaatcg ttcgcctcgt cgccgagcag gtcggcggca tccagcgaat accagacatc
     3781 cacgccgccc tgctccacgc gatccgcgac gtggcgcatc agctcgacgc tgcgcggatg
     3841 cggttcgccg gtttcgcggt gcacgaacag cgcgatcggc acgccccagg tgcgctggcg
     3901 cgagatcgtc cagtccgggc gcccgtcgat catgccggcg atgcgcgcct cgccccattc
     3961 cgggtaccag gtgacggtct tgatcgcagc cagcgcatcg cgacgcaggt gcgcctggtc
     4021 catcgagatg aaccattgcg gggtggcgcg gaatgcgacc ggcgtcttgt gccgccagca
     4081 atgcgggtag ctgtgcgtca gcttcgagaa cgccagcagg tgaccgctcg cgcgcagcac
     4141 ctcgaccagc acgtcgttgg ccttccacac atgcaagccg gcgagttcgg tgccatgcgc
     4201 cggcggcgtc gatggcaggt acacgccacg gccatcgacc gg
//
LOCUS       DAOOCX010000180         4235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_19748_length_4235_cov_7.304306, whole genome shotgun sequence.
ACCESSION   DAOOCX010000180 DAOOCX010000000
VERSION     DAOOCX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19748_length_4235_cov_7.304306"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PK399_11840"
     CDS             complement(<1..436)
                     /locus_tag="PK399_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW13852.1"
                     /translation="MTTRPLVLALAIAASLAAGCTQTSPAAKADAGTLRTLGSLAFKP
                     CSLPSPSPRGEPLEAQCTTFAVPEDRSKPGGRSIALNIAWIPPRNTGDPATDPVFFLA
                     GGPGQAAVATFPALAPVFNDVMKDRGIVLVDQRGTGKSNPLNC"
     gene            complement(463..657)
                     /locus_tag="PK399_11845"
     CDS             complement(463..657)
                     /locus_tag="PK399_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW13853.1"
                     /translation="MNNRMRELREAQGWSQGELAERLEVSRQTINALETGKYDPSLPL
                     AFRIARLFGQPIESLFIPED"
     gene            complement(654..1079)
                     /locus_tag="PK399_11850"
     CDS             complement(654..1079)
                     /locus_tag="PK399_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13854.1"
                     /translation="MTRLATRYWQSLSPALRRYYRASVAPCLVFTGLVVLHEWAARSG
                     QSAPLRAAFALGPVLAMAWMFTAYLRFLGDCDELERRIELGALAWAAGIVLHAAIGAL
                     LLLDAGVVAWSARHVAAWLCLLLLASYALVRGWLHRRYA"
     gene            complement(1076..1672)
                     /locus_tag="PK399_11855"
     CDS             complement(1076..1672)
                     /locus_tag="PK399_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13855.1"
                     /translation="MTLRRFMWLFLAFALGAFALAAAGRFLLDWPDNVVGMWAGIGAG
                     NLLGCLLFLLTPRWWRSHCDDMYAQPAGRRYLRALWPALVGYTLTLFASIFLVKRGIE
                     SVPLRGLVAVLPVLPIGMMMLAALRYLREIDELQRRIETDAIGVASLLVSLLYFAGGM
                     LQKAKVIELDAGVAMIWVFPLLCVTYGIAKMIVTRRYL"
     gene            complement(1679..1843)
                     /locus_tag="PK399_11860"
     CDS             complement(1679..1843)
                     /locus_tag="PK399_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13856.1"
                     /translation="MDKPKNLKGGGLAFLGSGVAFLGVAASGQPAFLGVAMAMIAMGI
                     VYLAKSRKAG"
     gene            complement(2243..2998)
                     /locus_tag="PK399_11865"
     CDS             complement(2243..2998)
                     /locus_tag="PK399_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPW13857.1"
                     /translation="MHFPLKLTARRMIAPSVAHLSFLRDDGQPLDYIPGQFIQVHFAY
                     ADGTAARRSYSLATQHDHALAPGEAVEIAVSYVPGGAATALFEGLPIGGSVDASGPFG
                     RFCLLPNDANRRYLLIGTGTGVTPYRAMLPLLASLIEARGIEVVLLQGARTPEELLYG
                     DEFRAFANAQPRFRFVPCFSRELPAAASPHAHADVRHGYVQAALAEFAPNAGGDIAYL
                     CGNPNMVDASFEALKEHGLPIPQIRREKYVSNK"
     gene            3128..4081
                     /locus_tag="PK399_11870"
     CDS             3128..4081
                     /locus_tag="PK399_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW13858.1"
                     /translation="MHPTTDGAPAPALRVSELRKTYDNGVEALKGVSLDVAPGDFHAL
                     LGPNGAGKSTLIGIVSSLVNKTAGKVEVFGIDLDADRDGAMRQIGLVPQEMNFNMFEK
                     PLDICVNYAGFYGIPRAQALVRAEEVLKDALLWDKADKMSRTLSGGMKRRLMIARAMM
                     TRPRLLILDEPTAGVDIEIRRGMWKTLKDINAAGTTIILTTHYLEEAESLCRNLAIID
                     RGTIVEHGPMKSLLAKLDVEGFLFDIDGNLPAQLPQIEGTTLVAADDHTLDMDMPRAM
                     DLNRVFAAFEAAGIRVRSMRTKSNRLEELFVRLTSKEGTSA"
     gene            4078..>4235
                     /locus_tag="PK399_11875"
     CDS             4078..>4235
                     /locus_tag="PK399_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW13859.1"
                     /translation="MSQIATTNSAASKNLVALGTIIRREVMRILRIWGQTLMPPAITM
                     TLYFLIFGG"
BASE COUNT          744 a         1436 c         1469 g          586 t
ORIGIN      
        1 tgcagttgag cgggttggac ttgccggtgc cgcgctggtc gaccagcacg atgccgcgat
       61 ccttcatcac gtcgttgaac accggcgcca gcgccgggaa ggtcgcgacc gctgcctgcc
      121 cgggaccacc ggccaggaag aacaccggat cggtcgccgg gtcgccggtg ttgcgcggcg
      181 ggatccacgc gatgttgagc gcgatgctgc ggccgcccgg cttgctgcgg tcttccggca
      241 cggcgaaggt ggtgcattgc gcttccagcg gctcgccccg cggcgagggc gagggcagcg
      301 aacagggctt gaatgccagg ctgccgaggg tgcgcagcgt gccggcatcg gccttggccg
      361 ccggcgatgt ctgcgtgcag ccggcggcca gcgaggccgc gatggccagc gccagcacga
      421 ggggacgggt ggtcatggtg aaactcctgc gggatgcatg gatcagtcct cggggatgaa
      481 aagggattcg atcggttgcc cgaacagccg ggcgatgcgg aaagcgagcg gcagcgacgg
      541 gtcgtacttg ccggtttcca gtgcgttgat ggtctgccgc gaaacctcca ggcgttcggc
      601 cagctcgccc tgcgaccagc cctgcgcctc gcgcagctcg cgcatgcggt tgttcatgcg
      661 tacctgcggt gcagccagcc gcgcaccagc gcataactgg ccagcagcaa caggcagagc
      721 cacgcagcga catgccgggc cgaccacgcg accacgccgg cgtcgagcag caacaacgcg
      781 ccgatcgccg catgcaggac gatgccggcg gcccaggcca gcgcgcccag ttcgatgcgc
      841 cgttccagtt cgtcgcaatc gcccaggaag cgcaggtagg cggtgaacat ccaggccatc
      901 gccagcaccg ggcccagcgc gaacgccgcg cgcaggggcg cggactggcc cgaccgagcc
      961 gcccattcgt gcagcacgac caggccggtg aagaccaggc acggtgccac ggaagcgcgg
     1021 tagtagcgcc gcagcgcggg gctcaggctc tgccagtagc gggtggccag gcgggtcaca
     1081 ggtagcgccg ggtcacgatc atcttggcga tgccgtaggt cacgcacagc agcgggaaca
     1141 cccagatcat cgccaccccg gcgtccagct cgatgacctt ggccttctgc agcatgccgc
     1201 cggcgaaata cagcagggac accagcaggc tggcgacgcc gatcgcatcg gtttcgatgc
     1261 gtcgctgcag ctcgtcgatc tcgcgcaggt agcgcagcgc ggccagcatc atcatgccga
     1321 tcggcagcac cggcagcacc gccaccagcc cgcgcagcgg gacggactcg atgccgcgct
     1381 tgaccaggaa gatcgaggcg aacagcgtca gcgtgtagcc aaccagggcc ggccacagcg
     1441 cgcgcaggta gcggcgcccg gccggctgcg cgtacatgtc gtcgcagtgc gagcgccacc
     1501 agcgcggcgt cagcaggaac aggaggcacc caagcaagtt accggccccg attccggccc
     1561 acatgccgac cacgttgtcc ggccagtcca gcaggaaccg gccggcggcg gccagggcga
     1621 acgcgccgag cgcgaacgcc aggaagagcc acatgaaacg ccgtagggtc atggcgggtc
     1681 agccggcctt gcgcgacttg gccaggtaca cgatgcccat ggcaatcatc gccatcgcca
     1741 cgcccaggaa cgcgggctgc ccgctggcgg ccacccccag gaaggccacg ccggagccca
     1801 ggaacgccag gccgccacct ttcaggttct tcggtttgtc catcgccgtg ctccgttgct
     1861 gtcaaggacg cttgacacaa cgatacggac atcgcgaaac gcttgtcaag cgtcctttac
     1921 agccagcatt gcagcatgga aggcgtatga cggggtctgc ggaaagcgcg ccatgggtga
     1981 gcacgctttg cctgcatcgc gtgcgatgcg ggcgtgcgaa cgcccggcac gctgtgccgg
     2041 gcagggccgc gtagcgggcg cgcggcggca catcgaacag gatgttcgca ctaagccggg
     2101 tagcaggccc cggcatacgc ctggcgattc acttgttgct gacgtacttc tcgcggcggg
     2161 tgagggcggg cgatagcgcg gcatgccgcg cgcccggacg aacgccgcgc acgaagtgcg
     2221 tggccgggga tccgccgccg gcctacttgt tgctgacgta cttctcgcgg cggatctgcg
     2281 ggatcggcag gccgtgctcc ttcagcgcct cgaaactggc atcgaccatg ttcgggttgc
     2341 cgcacaggta ggcgatgtcg cccccggcat tcggcgcgaa ctccgccagc gcggcctgca
     2401 cgtagccgtg gcggacgtcg gcatgtgcgt gcggcgaggc ggcagccggc aactcgcgcg
     2461 agaagcacgg gacgaaacgg aaccgcggct gcgcgttcgc gaaagcacgg aattcgtcgc
     2521 cgtacaacag ttcctccggg gtgcgcgcac cctgcaggag caccacctcg atgccgcgcg
     2581 cttcgatcag cgaggcgagc agcggcaaca tcgcccggta cggcgtcacc ccggtgccgg
     2641 tgccgatcag caggtagcgg cggttggcgt cgttgggcag caggcagaac cggccgaacg
     2701 ggccgctggc atccacgctg ccgccgatcg gcaggccctc gaacagcgcc gtcgccgcgc
     2761 cacccgggac atagctgacc gcgatctcca ccgcttcgcc gggggccagc gcgtggtcgt
     2821 gctgggtcgc cagcgaatag ctgcggcgcg ccgcggtgcc gtcggcgtag gcgaagtgga
     2881 cctggatgaa ctggccgggg atgtaatcca gcggctggcc gtcgtcgcgc aggaacgaca
     2941 ggtgggcgac ggagggcgcg atcatccggc gcgcggtcag cttcaggggg aaatgcacga
     3001 tggccaagcg ctgcgatgct ctgtttggtg gatgcaacgc cgcgccgggc attgccccgc
     3061 aggcggccgc ctatactaac cgcaacattt tcctgcctcg ccgcggtccc gcggccgcgg
     3121 agtcccgatg cacccgacca ccgacggcgc gcctgcgccc gcgctgcgcg tcagtgagct
     3181 gcgcaagacc tacgacaacg gcgtggaagc gctgaagggc gtcagcctgg acgtcgcgcc
     3241 cggcgatttc catgccctgc tggggccgaa cggcgccggc aagtcgaccc tgatcggcat
     3301 cgtcagttcg ctggtcaaca agactgccgg caaggtcgag gtattcggca tcgacctcga
     3361 cgccgaccgc gacggggcga tgcgccagat cggcctggtg ccgcaggaaa tgaacttcaa
     3421 catgttcgag aagccgctgg acatctgcgt gaactacgcg ggcttctacg gcatcccgcg
     3481 cgcgcaggcg ctggtccgcg cggaggaagt gctgaaggac gcgctgctct gggacaaggc
     3541 cgacaagatg agccgcacgc tgtccggcgg catgaagcgg cggctgatga tcgcccgcgc
     3601 gatgatgacc cgcccgcgcc tgctgatcct ggacgagccg accgccggcg tggacatcga
     3661 gatccgccgc ggcatgtgga agacgctgaa ggacatcaac gcggccggca ccacgatcat
     3721 cctgaccacc cactacctgg aggaagcgga gagcctgtgc cgcaacctcg cgatcatcga
     3781 ccgcggcacc atcgtggaac acgggccgat gaagtcgctg ctggccaagc tggacgtcga
     3841 aggctttctg ttcgacatcg atggcaacct gcccgcgcaa ctgccgcaga tcgaaggcac
     3901 caccctggtc gcggccgacg accacaccct ggacatggac atgccgcggg cgatggacct
     3961 caaccgggtg ttcgccgcgt tcgaggccgc cggcatccgg gtgcgctcga tgcgcaccaa
     4021 gagcaaccgg ctggaggaac tgttcgtgcg cctgaccagc aaggaaggaa cgtccgcatg
     4081 agccagatcg ccaccaccaa ttccgcggcc agcaagaacc tggtcgcgct gggcaccatc
     4141 atccgccgcg aagtgatgcg catcctgcgc atctggggcc agaccctgat gccgccggcg
     4201 atcaccatga ccctgtactt cctgatcttc ggcgg
//
LOCUS       DAOOCX010000181         4204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_19943_length_4204_cov_10.349000, whole genome shotgun
            sequence.
ACCESSION   DAOOCX010000181 DAOOCX010000000
VERSION     DAOOCX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19943_length_4204_cov_10.349000"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PK399_11880"
     CDS             complement(<1..1123)
                     /locus_tag="PK399_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13860.1"
                     /translation="MSRFDASARTVLFAALLAALPAFAQSAEVKREVVGNRTSENIPE
                     IPAALLEQLNRYQNTRGAGLAGWTKDGCLLISTRFAETAQAHRVCQPLGMREQLTFYP
                     EPVGGLTPAPAKAWRDGFVFAKDKGGDEFSQLYWFDNDSRSVTLLTDGKRSQNGGTTL
                     SRDGGLMAYSSTSRNGTDRDVWLRNTRSGETKLLVDAGGNWSPMDFSPDGSRLLVMKY
                     VSAAESYPGVVDVATGKLALFPVDGGKASFGGFAFAPDGKAVYFISDEPLRGKPQEFQ
                     TLRYHDPASGTLEVLSSRIAWDIEGFTLADDGRHLAYVSNEDGISKLHVLSLPSHNEI
                     RLPALPIGVIGGIAFSPDGKRLAFSLNSATSPSDVHVIDL"
     gene            complement(1245..2975)
                     /gene="dnaG"
                     /locus_tag="PK399_11885"
     CDS             complement(1245..2975)
                     /gene="dnaG"
                     /locus_tag="PK399_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009605376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPW13861.1"
                     /translation="MARIPDAFIDDLLARTDIVEVIGARVPLKRQGKEYAARCPFHDE
                     RSASFTVSQTKQFYHCFGCGAHGTAISFMMNFDRLEFLDAVDELAKRVGMEIPRDTQQ
                     RNANPETRDLYGAMEAASTFFRSQLANSEKALAYVEKRGIDPAIVERYAIGYAPDGFS
                     ALRDALGSDPGRMQLMERGGLFSKNDRGNVYDKFRDRLMFPIHDRRGRTIAFGGRVID
                     PEDSPKYLNSPETALFHKGRELYGLWQAKQANAKLDRLIVVEGYMDVVALAQYGVSQA
                     VATLGTATTPDHAELLFRNAPDVYFCFDGDRAGRSAAWKALESVLPRMKDGRQAFFLF
                     LPDGEDPDSIVRQEGVAGFDARLRDATPLSEFFYASLSSDVNLSSLDGKARLAERCKP
                     LLAQIPDGAFGDLMQQRLTELTGVGARASNPQTHVPTKRATQSSRGTPAQKPSLVRGA
                     ITHLLHRPALALDLQPPYRFAALRQPGIDLLSELVLLVRERPEISTGALLEHFEGREE
                     AAALQKLATLDLPGEAANLRDEFLDAITQLDRLVMQQRLEELQQQQRDGGLDAEGKQE
                     MRELLQAQRR"
     gene            complement(3000..3503)
                     /locus_tag="PK399_11890"
     CDS             complement(3000..3503)
                     /locus_tag="PK399_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW13862.1"
                     /translation="MAAAHPLLSIRPATADDVPLIRQLIAELAEYERLADAAVATDDG
                     LREQLFGAHPAAEVLIGEVDGEPAGFALFFHNFSTFLGKRGLYLEDLFVRPAFRGAGL
                     GKHLMASLARLAVQRDCGRFEWSVLDWNAPAIGFYRTLGAVGMAEWTVQRLEGEALHA
                     LASHDAL"
     gene            complement(3509..3952)
                     /locus_tag="PK399_11895"
     CDS             complement(3509..3952)
                     /locus_tag="PK399_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPW13863.1"
                     /translation="MSLKQQLTDDMKTAMKSGEKDRLGVIRLVLAAIKQKEVDERIEM
                     TDPLVLAVLEKMVKQRKDSISQYEAAAREDLAAIERYELGIIDAYLPMKMDEAAILAE
                     IDKAIAATGAASGADIGKLMGVLKPALAGQADMGLVSKLVKQRLG"
     gene            complement(4073..>4204)
                     /gene="rpsU"
                     /locus_tag="PK399_11900"
     CDS             complement(4073..>4204)
                     /gene="rpsU"
                     /locus_tag="PK399_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPW13864.1"
                     /translation="LAETRKREFYEKPTQERKRKAAAAVKRQARRAGRDVTKRQRLY"
BASE COUNT          649 a         1380 c         1434 g          741 t
ORIGIN      
        1 ccaggtcgat cacgtgcacg tcgctgggcg aggtcgcgct gttcaggctg aatgcgagcc
       61 gcttcccgtc cggcgagaat gcgatgccgc caatcacccc gatcggcagg gcgggcaggc
      121 gaatctcgtt gtgcgacggc aacgacagca catgcagttt gctgatgccg tcctcgttgc
      181 tcacgtaggc gaggtgcctg ccgtcatcgg ccagggtgaa gccttcgata tcccaggcaa
      241 tgcgggagct cagcacctcc aaggttccgc tggcggggtc gtggtaacgc agggtctgga
      301 attcctgtgg cttgccccgc agcggttcgt cggagatgaa gtacaccgcc ttgccgtcgg
      361 gcgcgaaggc gaaaccgccg aagctggcct tgccgccatc gaccgggaac aacgccagct
      421 tgccggtggc gacatcgacc acgcccggat acgactcggc ggcggagacg tacttcatca
      481 ccagcaggcg cgacccgtcc ggcgagaaat ccatcggcga ccagttgccg ccggcatcga
      541 ccagaagctt cgtctcgccg ctgcgggtat tgcgcagcca cacgtcgcga tcggtgccgt
      601 tgcgcgaggt gctgctgtac gccatcagcc cgccgtcgcg gctgagcgtg gtgccgccgt
      661 tctggctgcg cttgccatcg gtgagcaacg tgaccgaacg ggagtcgttg tcgaaccagt
      721 acaactggct gaactcgtcg ccgcccttgt ccttggcgaa cacgaagccg tcgcgccacg
      781 ccttcgccgg cgcgggcgtg aggccgccga ccggctccgg atagaaggtc agttgctcgc
      841 gcatccccag tggctggcag acgcgatgcg cctgcgcggt ctcggcgaag cgggtgctga
      901 tcagcaggca gccgtccttc gtccatcccg cgagcccagc gccgcgggtg ttctggtagc
      961 ggttgagttg ttcgagcaac gctgccggga tctccgggat gttctcgctg gtgcgattgc
     1021 cgacgacctc gcgcttcact tcggcgcttt gtgcgaaggc gggcagggcc gcgagcagcg
     1081 cggcgaacag gacggtgcgg gcactcgcgt cgaagcggga catggcggct tccccaagcc
     1141 tggttgaagc tgcagcttaa cgcgctggcg tttcgccgcc tgcgtcgaag gtcatggggt
     1201 tttgctcggc acgcccgtcc tcgcatccga cgggtgagct tccactagcg acgttgcgcc
     1261 tgcagcaatt cgcgcatttc ctgcttgccc tcggcatcca gtccgccatc gcgctgctgc
     1321 tgttgcagtt cctcgagtcg ttgctgcatc accagccggt cgagctgggt gatggcatcc
     1381 aggaattcgt cgcgcaggtt ggccgcttcg ccggggagat ccagcgtcgc cagcttctgc
     1441 agggcagcgg cttcctcgcg gccctcgaag tgttcgagca gggcgccggt gctgatctcg
     1501 ggacgttcgc gcaccagcag cacgagctcc gacaacaggt cgatgccggg ctgtcgcaac
     1561 gcggcgaagc ggtacggcgg ctgcaggtcc agcgccaggg cggggcggtg cagcagatgg
     1621 gtgatcgcgc cgcgcaccag gctgggtttc tgcgcaggcg tgccgcgcga cgattgcgtc
     1681 gcacgcttgg tggggacgtg tgtctgtgga ttgcttgcac gcgcgccgac accggtgagt
     1741 tcggtcaggc gctgctgcat gaggtcgcca aaggcgccat cgggaatctg cgcaagcaag
     1801 ggtttgcagc gttcggcgag gcgcgccttg ccgtcgagac tcgacaggtt cacgtcgctg
     1861 gacagcgagg catagaagaa ctcggacagc ggcgtggcat cgcgcaggcg cgcatcgaag
     1921 ccggccacgc cttcctggcg cacgatcgaa tccggatcct cgccatccgg caggaacagg
     1981 aagaacgcct ggcggccatc cttcatccgc ggcagcacgg actccagcgc cttccacgcg
     2041 gcgctgcgac ccgcgcggtc gccatcgaag cagaaataga catccggcgc gttgcggaac
     2101 agcagttccg cgtggtcggg cgtggtcgcg gtaccgagcg tggcgaccgc ctgggagacg
     2161 ccgtactgcg ccagcgccac cacgtccatg tagccctcga ccacgatcag gcggtcgagc
     2221 ttggcgttcg cctgcttcgc ctgccacagg ccgtagagct cgcggccctt gtggaacagc
     2281 gcggtctccg gcgagttgag gtacttcggc gaatcctcgg gatcgatgac gcggccaccg
     2341 aacgcgatgg tgcggccgcg gcggtcgtgg atcgggaaca tcaggcgatc gcggaacttg
     2401 tcgtagacgt tgccgcggtc gttcttcgag aacaggccgc cgcgttccat caactgcatg
     2461 cgcccggggt cgctgcccag cgcatcgcgc aatgcggaaa acccatcagg cgcgtagccg
     2521 atcgcgtagc gttcgacgat ggcggggtcg atgccgcgtt tctcgacgta cgcaagcgcc
     2581 ttctcgctgt tcgccagttg gctgcggaag aacgtggatg ccgcctccat cgcgccgtac
     2641 aagtcgcgcg tctcgggatt ggcgttgcgc tgctgggtgt cgcgcgggat ctccatgccc
     2701 acgcgcttgg ccagttcgtc gaccgcgtca aggaactcga ggcggtcgaa attcatcatg
     2761 aaactgatcg cggtgccgtg cgcgccgcaa ccgaagcagt gatagaactg cttggtctgg
     2821 gagaccgtga agctggccga gcgttcgtcg tggaacggac aacgcgccgc gtattccttg
     2881 ccctgccgct tcagcgggac gcgtgcgccg atgacttcga cgatgtcggt acgcgccagc
     2941 agatcatcga tgaacgcgtc ggggatgcgg gccatcggat tagcttgcca cgcgcgctgt
     3001 cagagcgcgt cgtgcgaagc cagcgcgtgc agggcctcgc cttccagccg ctgcacggtc
     3061 cattcggcca tgcccaccgc accgagcgtg cgatagaagc cgatcgccgg cgcattccag
     3121 tccagcacgc tccattcgaa gcggccgcag tcgcgctgca ccgcaaggcg cgccagtgac
     3181 gccatcaggt gcttgcccag gccggcgcca cggaaggccg ggcgcacgaa caggtcttcc
     3241 aggtacaggc cgcgcttgcc gaggaaagtg ctgaagttgt ggaagaacag ggcgaatcct
     3301 gccggttcgc catcgacctc gccgatcagc acttccgccg ccgggtgcgc gccgaacaac
     3361 tgttcgcgca ggccgtcgtc ggtggccacc gcggcatccg ccagtcgttc gtactcggcc
     3421 agctcggcga tcaactgccg gatcagcggc acgtcgtcgg cggtggcggg acggatcgac
     3481 agcagcgggt gcgcggcggc catggccgtc agcccaggcg ctgcttcacg agcttcgaca
     3541 ccaggcccat gtcggcctgt ccggccagcg ctggcttgag cacgcccatc agcttgccga
     3601 tgtcggcgcc agacgccgcg ccggtcgccg cgatggcctt gtcgatctcc gccaggatcg
     3661 cggcttcgtc catcttcatc ggcagatagg catcgatgat gcccagctcg tagcgctcga
     3721 tcgcggcgag gtcctcgcgt gcggcggctt cgtactggct gatcgagtcc ttgcgctgct
     3781 tgaccatctt ctccagcacg gccagcacca gcggatcggt catctcgatg cgctcgtcga
     3841 cttccttctg cttgatcgcg gccaggacca ggcggatcac gcccaggcga tccttctcgc
     3901 cggacttcat cgcggtcttc atgtcgtcgg tgagttgctg cttgaggctc atgctggctc
     3961 cggggggatc gactggggcg ggtggatcag gggtgccggg atcggcggtc gcagaaacgc
     4021 gaaaagccgg ccacgcttgc gcgtgccggc ttttgctgcc acgaaaccgc gctcagtaca
     4081 ggcgctggcg cttggtgacg tcgcgaccgg cgcgacgcgc ctggcgcttc acggcagccg
     4141 ccgccttgcg cttgcgctcc tgggtcggct tctcgtagaa ctcgcgcttg cgggtctcgg
     4201 cgag
//
LOCUS       DAOOCX010000182         4177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_20115_length_4177_cov_5.882339, whole genome shotgun sequence.
ACCESSION   DAOOCX010000182 DAOOCX010000000
VERSION     DAOOCX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20115_length_4177_cov_5.882339"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /gene="cysW"
                     /locus_tag="PK399_11905"
     CDS             complement(<1..362)
                     /gene="cysW"
                     /locus_tag="PK399_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Part of the ABC transporter complex cysAWTP
                     involved in sulfate/thiosulfate import; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HPW13865.1"
                     /translation="MADIPSSPLRDPLQEPAWLRALLIGAAVLSMALLVVLPLAMVLA
                     SAFGDGFRAWWAALAEPDALAALRLTLFTAAIVIPVNAVFGVFAAWALTRFEFRGKRV
                     LLAFIDLPFAVSPVVAGL"
     gene            complement(355..1212)
                     /gene="cysT"
                     /locus_tag="PK399_11910"
     CDS             complement(355..1212)
                     /gene="cysT"
                     /locus_tag="PK399_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HPW13866.1"
                     /translation="MSSLASPSTPTRNRSPLPGFGLSLGLGMAWLGVVVLLPLAALAV
                     RAAGLGPQGWLRALRDERVQEALKLSFGASLTAAVIASVVGALVAWVLVRYRFTGRRL
                     LDALVDLPFALPTAVAGIALTAIYAPNGWVGKLLVAYDIKVAYAVPGIVLALVFIGLP
                     FTVRTLQPVLETLGREPEEASASLGASRFTTFRRVIVPELLPAWLTGFSLAFARSLGE
                     YGSVIFIAGNLPYKTEIAPLLITIRLEEYDYNGAVAIAALLLVASFVCLLAINLIPLL
                     FEKKAKRNG"
     gene            complement(1226..2206)
                     /locus_tag="PK399_11915"
     CDS             complement(1226..2206)
                     /locus_tag="PK399_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPW13867.1"
                     /translation="MALALPAFAGLANGKDKELLNVSYDPTREFYAEFNKAFAADWKA
                     KTGETVAIRASHGGSGKQARSVIDGLEADVVTLALSADIDVLATNGKLLPANWQARLP
                     HNSAPYTSTIVFLVRKGNPKGIKDWGDLAKPGIGVITPNPKTSGGARWNYLAAWAWAS
                     REYRDGDKVVGYLTRLFKNVPVLDTGARGSTTTFVERGIGDVLLAWENEALLTLNDAT
                     TRGKFEIVVPSISIKAEPPVAWVDKNVAKHGTRKQAEAYLRFLYSAKGQALAAKHFYR
                     PAEPDKVPAALLAQFPEVSQVTIDGAFGGWKKAQAEHFGDGGFFDRIYKP"
     gene            complement(2289..3707)
                     /locus_tag="PK399_11920"
     CDS             complement(2289..3707)
                     /locus_tag="PK399_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPW13868.1"
                     /translation="MPLKRTLLALAIGLAVLPAHAADPGDATIQELLARIAALEARLA
                     AVEGRTEIPEVAEVDQRLRVVERKQELQVEADAARATTATTLALDAGKGLSVKAPGGL
                     EVKLRGLLQGDARFFANGSSQNDGFLLRRVEPTIEGSWGIASFRITPQLAGDSATIND
                     AYIDLKFDPRASVRIGKAKAPFGLERLQSSASTAQVELGLPSELAPGRDIGVQLSGDV
                     AGGALNYGIGVYNGAVDGRDGLTANPDGEFEYVGRLFWEPFKNSASNTGTLGIGVAAS
                     VGDTVGSGNNFLPRYRTPGQEQFFGYGSNIAADGERLRKTVQCYYYRGTFGVLGEWIE
                     SSQDVRVASGTGAGTRATLRNDAWQISGGWVLTGEDAGYRGVVKPSSPFAIGGDGWGA
                     LELAARYGRLEIDEGAFPLFANAASSAQAIRSWGLGLNWYLAQNFKLVTNYTQASFDG
                     GAAAGADRADEKALFTRAQLSF"
     gene            complement(3790..3975)
                     /locus_tag="PK399_11925"
     CDS             complement(3790..3975)
                     /locus_tag="PK399_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HPW13869.1"
                     /translation="MSANPSSSIASPSASTQCGAPEVEAEVLRAIRDIQFGTVEIVVH
                     QSRVVQITRSEKVRFER"
     gene            complement(3972..>4177)
                     /locus_tag="PK399_11930"
     CDS             complement(3972..>4177)
                     /locus_tag="PK399_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13870.1"
                     /translation="LAPVRCASKSAYEPCADCIDPEHCAVRALMRETREALSGVLDRH
                     SLLTLSQRTDAEPRQRPSTGDKP"
BASE COUNT          700 a         1413 c         1389 g          675 t
ORIGIN      
        1 cacaggccgg cgaccaccgg cgacaccgcg aagggcaggt cgatgaaggc cagcaagacg
       61 cgcttgccgc ggaactcgaa gcgggtcagc gcccacgccg cgaacacgcc gaacaccgca
      121 ttgaccggga tcacgatggc agcggtgaac aacgtcagcc gaagcgcggc gagcgcatcg
      181 ggttcggcca gcgccgccca ccaggcgcgg aagccgtcgc cgaacgcgga ggccaacacc
      241 atcgccagcg gcaacaccac cagcaaggcc atcgacagca ccgcggcacc gatcagaagc
      301 gcgcgcagcc aagccggctc ctgcagcgga tcgcgcaacg gcgaacttgg aatgtcagcc
      361 attgcgcttg gccttcttct cgaacagcag cggaatcagg ttgatcgcca gcaggcagac
      421 gaaggacgcc accagcagca atgcggcgat cgccaccgcg ccgttgtagt cgtattcctc
      481 caggcggatc gtgatcagca gcggcgcgat ctcggtcttg tatggcaggt tgccggcgat
      541 gaagatcacc gagccgtatt cgcccaggct gcgcgcgaac gccagcgaga atccggtcaa
      601 ccacgctggc aacaattccg ggacgatcac gcggcggaac gtggtgaagc ggctggcgcc
      661 gagcgaggcc gacgcttcct ccggctcgcg acccagggtt tccagcaccg gttgcagcgt
      721 gcgcacggtg aacggcagac cgatgaagac cagcgccagc acgatgcccg gcaccgcata
      781 ggcgaccttg atgtcgtatg cgacgagcaa tttgccgacc cagccgttcg gcgcgtagat
      841 cgcggtcagc gcgatgccgg ccaccgcggt cggcaatgcg aacggcaggt cgaccaacgc
      901 atccagcaaa cgccggcccg tgaagcgata gcgcaccagc acccaagcca ccaacgcgcc
      961 gaccaccgac gcaatcaccg ctgcagtgag cgaagcgccg aagctcagct tcagcgcctc
     1021 ctgcacgcgc tcgtccctga gcgcgcgcag ccacccttgc ggccccaacc cggccgcacg
     1081 caccgccaat gctgcaagcg gcaacagcac caccacgcca agccacgcca tgcccaggcc
     1141 aaggcttagc ccaaaccccg gcagcgggct gcgattgcgc gtgggcgtcg acggcgatgc
     1201 cagcgaactc atcgtcttgt gcctatcaag gcttgtaaat gcgatcgaag aagccgccat
     1261 cgccaaagtg ctcggcctgt gccttcttcc agccgccgaa cgcgccgtcg atggtcacct
     1321 gcgagacctc cgggaactgc gcgagcagcg ccgccggcac cttgtccggc tcggcggggc
     1381 gatagaaatg cttcgcggcc agcgcctgcc ccttcgccga atacaggaag cgcaggtagg
     1441 cctcggcctg cttgcgggtg ccgtgcttgg ccacgttctt gtcgacccag gccaccggcg
     1501 gttccgcctt gatgctgatc gacggcacca cgatctcgaa cttgccgcgg gtggtggcgt
     1561 cgttgagcgt cagcagggct tcgttctccc aggccagcag cacatcgccg atcccgcgtt
     1621 cgacgaaggt ggtggtggag ccacgggcac ccgtatccag caccggcacg ttcttgaaca
     1681 accgggtcag gtagcccacc accttgtcgc catcgcgata ttcgcgcgat gcccaggccc
     1741 acgccgccag gtagttccag cgcgcgccgc ccgaggtctt cggattcggc gtgatcaccc
     1801 cgatcccggg ctttgccagg tcgccccagt ccttgatgcc cttcgggttg cccttgcgca
     1861 caagaaagac aatggtcgag gtgtacggcg cgctgttgtg cggcaagcgc gcctgccagt
     1921 tggccggcag cagcttgccg ttggtggcca gcacgtcgat gtcggcggac agcgccagcg
     1981 tcaccacgtc ggcttccagg ccatcgatga ccgagcgcgc ctgcttgccc gagccgccat
     2041 gcgaggcgcg gatggccacc gtctcgccgg tctttgcctt ccaatctgcg gcgaatgcct
     2101 tgttgaattc cgcgtagaac tcgcgggtgg ggtcgtagga gacgttgagc agttccttgt
     2161 ccttgccatt ggccaggccg gcgaatgccg gcagcgccag cgccatgccg aacagcaggt
     2221 tcctgagcgt cgtgcgcgtg tcgtgatggg tcttcatggt gtgatcctcg tggggttcgc
     2281 gggatgcgtc agaaggaaag ttgcgcgcgg gtgaacagcg ctttttcgtc ggcgcgatcg
     2341 gcgcccgctg cggcccctcc gtcgaagctg gcctgggtgt aattggtgac cagcttgaag
     2401 ttctgcgcga ggtaccagtt caggcccagg ccccagctgc ggatcgcttg cgcggaactg
     2461 gcggcgttcg caaacagcgg gaacgcgccc tcgtcgatct ccagccggcc atagcgtgcg
     2521 gcgagttcca gtgcgcccca cccgtcgccg ccgatcgcga acggcgacga cggtttcacc
     2581 acgccgcgat aacctgcgtc ttcgccggtc agcacccaac caccactgat ctgccaggcg
     2641 tcgttgcgca gcgtcgcgcg cgtgccggcg ccagtgccac tggcgacccg cacgtcctgc
     2701 gatgactcga tccattcacc caacacgccg aacgtgccgc ggtagtaata gcactgcacg
     2761 gtcttgcgca ggcgctcgcc atcggcggcg atgttgctgc cgtagccgaa gaactgctcc
     2821 tgcccgggcg tgcgatagcg cggcaggaaa ttgttgccgc tgcccacggt gtcgccgacg
     2881 ctcgccgcca cgccgatgcc gagcgtgccg gtgttgctgg cgctgttctt gaacggttcc
     2941 cagaacaacc ggccgacgta ctcgaactcg ccgtccggat tggcggtgag cccgtcgcgg
     3001 ccatcgaccg cgccgttgta cacgccgatg ccgtagttga gcgcaccgcc ggccacgtcg
     3061 cccgacagct gcacgccgat gtcgcggccg ggcgcgagct cggacggcaa gcccagttcg
     3121 acctgcgcgg tggacgcact cgattgcagg cgctccaacc cgaatggcgc cttggccttg
     3181 ccgatccgca cgctggcgcg cggatcgaac ttgaggtcga tgtaggcgtc gttgatggtc
     3241 gcgctgtcgc cggcgagctg cggggtgatg cggaacgagg cgatgcccca gctgccttca
     3301 atggtcggtt cgacccggcg cagcaggaag ccgtcgttct gcgacgatcc gttcgcgaag
     3361 aagcgcgcgt cgccctgtag cagcccgcgc agcttcactt ccaggccgcc cggcgccttg
     3421 accgagagcc ccttgccggc atccagcgcc agcgtggtgg cggtggtcgc cctggcggcg
     3481 tcggcttcga cctgcagttc ctgcttgcgt tcgaccacgc gcaagcgctg gtcgacctcg
     3541 gcgacctctg gaatctcggt acgaccctcg accgccgcaa gtcgtgcttc caatgcggcg
     3601 atgcgggcca ggagttcctg gatagtggca tcgccgggat ctgcggcgtg cgcgggcagg
     3661 actgcaagcc cgatggcgag cgccaggagt gtgcgtttca gtggcatgcg aacctcgatg
     3721 ggtgagtgac atgcctccgg tcgaccggtc ggcgggttgc ggacgtggcg caggggggag
     3781 cgccgtggat tagcgttcga agcggacttt ctcgctgcgg gtgatctgca ccacgcggga
     3841 ttggtggacc acgatctcga cggtcccgaa ctggatgtcg cggatcgcgc gcaggacttc
     3901 ggcctccact tcgggagcgc cgcattgtgt cgaggcagat gggctggcga tggacgacga
     3961 tgggttcgca ctcatggctt gtctccggtg gaggggcgct gacgtggttc ggcgtcggta
     4021 cgctggctga gggtgagcag gctgtgccgg tcgagcacgc cggacagcgc ttcgcgggtt
     4081 tcgcgcatca acgcgcgtac cgcgcaatgc tcgggatcga tgcaatcggc gcagggttcg
     4141 taggcacttt tgctggcaca gcgcaccggc gccagcg
//
LOCUS       DAOOCX010000183         4170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_20170_length_4170_cov_6.977157, whole genome shotgun sequence.
ACCESSION   DAOOCX010000183 DAOOCX010000000
VERSION     DAOOCX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20170_length_4170_cov_6.977157"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PK399_11935"
     CDS             complement(<1..1081)
                     /locus_tag="PK399_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW13871.1"
                     /translation="MSEVQNFDVVVIGAGPAGYHAAIRAAQLGLKTACIDAALGKDGK
                     PALGGTCLRVGCIPSKALLDSSRQFWNMGHIFDQHGISFEKPAIDVARMVARKDGIVK
                     QFTGGIAALFKANKVSAFHGFGQLQPGNIVKVKQHDGSEVELKGTNVILAAGSDSIEL
                     PFAKFGEHIIDNVGALDLETVPKRLGVIGAGVIGLELGSVWKRLGSEVTILEGLPNFL
                     AAADAEVAKVAAREFKKQGLDIRLGCKCTATEVKADGVHISYTDDKGAAQELVVDKLL
                     VAVGRRAASKGLLADGTGVKLNERGQVEVDEHCHTGVDGVWAIGDCVRGPMLAHKGFE
                     EGIAVAELIAGLPGHVNFDTIPWVIY"
     gene            complement(1138..2358)
                     /gene="sucB"
                     /locus_tag="PK399_11940"
     CDS             complement(1138..2358)
                     /gene="sucB"
                     /locus_tag="PK399_11940"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HPW13872.1"
                     /translation="MATEVKVPVLPESVSDATIATWHKKAGDAVKRDENLLDLETDKV
                     VLEVPSPVDGVLKEIKFEAGATVTSQQLIAVIEEGAVAAAPAAEAAKAAPAAGADEVQ
                     PKAQAAAAHAAAPASTTPAPAAGDLPPGARFAATTAGVDPAQVEGTGRRGMVTKEDIV
                     NYASGKTAGVSGGARPEERVPMTRMRTRIAERLMQSKNSIAMLTSFNEVNLGKVMAMR
                     KELGESFEKANGIKLGFMSFFAKAAANALQRHPIVNASVDGSDVIYHGYADISIAVST
                     DKGLVTPVLRNVERMGFADIESAIAGYAKKARDGKLGLDDLQGGTFTITNGGTFGSLM
                     STPIINPPQSAILGMHAIKERAIVENGQVVAAPMMYIALSYDHRIIDGKDAVLFLVDI
                     KNQLENPHRMLLGM"
     gene            complement(2405..>4170)
                     /locus_tag="PK399_11945"
     CDS             complement(2405..>4170)
                     /locus_tag="PK399_11945"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HPW13873.1"
                     /translation="FAGQGVVMELFQMSQARGFRVGGTVHVVINNQVGFTTSAAEDAR
                     STLYCTDVAKMIGAPILHVNGDDPEAVAFVSEMAFDFRQRFGKDVVIDLVCYRRHGHN
                     EADEPAATQPLMYQKIRAMKTTRELYAARLDGEGSVPAAASQALVDGYRNTLDAGDVT
                     TDVVQVKADEFTIDWSPYLSAKLSDPVDTRVERATLDALATRINAIPEAVKLHPRVAK
                     IYEDRRKMAAGEQAGDWGFAENLAYATLVNEGYRLRLVGQDCGRGTFFHRHAILHDQS
                     TDNYYLPLRELLKDTPARSPEDVTIIDSLLSEEAVMAFEYGYATADPMTLDIWEGQFG
                     DFANGAQVVIDQFLSSGEAKWDRLCGLALFLPHGYEGQGPEHSSARLERFLQLCALEN
                     MIVCAPTTPAQDFHMIRRQMRMGTRKPLVVMTPKSLLRHKLAVSTLDELANGEFQRLI
                     PDTNATSKKVRRVVVCSGKVYYDLLDEAQKQGITDVALVRAEQLYPFPRPELAAELKR
                     FASANEVVWCQEEPQNQGAWYQIRHHLQACLQGKQTLTYAGRPRSPSPAAGHLAEHVA
                     EQNKLVADALVNPAGAEITAE"
BASE COUNT          614 a         1377 c         1401 g          778 t
ORIGIN      
        1 tgtagatcac ccacggaatg gtgtcgaaat tgacgtggcc gggcaggccg gcgatcagtt
       61 ccgcaaccgc gatgccttcc tcgaagccct tgtgggcgag catcggcccg cgcacgcagt
      121 cgccgatcgc ccatacgccg tccacgccgg tgtggcagtg ctcgtccact tccacctggc
      181 cacgctcgtt gagcttcacg ccggtgccgt ccgccagcag gcccttgctc gccgcgcggc
      241 ggcccacggc caccagcagc ttgtcgacca ccagttcctg cgccgcgccc ttgtcgtcgg
      301 tgtagctgat gtgcacgcca tcggccttga cctcggtggc ggtgcacttg cagcccaacc
      361 tgatgtccag gccctgcttc ttgaactcgc gcgcggcgac cttcgccact tcggcgtcgg
      421 cggcggcaag gaaattcggc aggccttcca ggatggtcac ttcggacccc aggcgcttcc
      481 acacgctgcc cagctccagc ccgatcacgc cggcgccgat cacgcccagg cgcttcggca
      541 cggtctcgag atccagcgcg ccgacgttgt cgatgatgtg ctcgccgaac ttcgcgaacg
      601 gcagttcgat cgagtccgaa ccggccgcca ggatcacgtt ggtgcccttc agttcgactt
      661 cgctgccatc gtgctgcttc accttgacga tgttgcccgg ctgcaactgg ccgaagccat
      721 ggaacgcgct caccttgttc gccttgaaca gcgcggcgat gccgccggtg aactgcttca
      781 cgatgccgtc cttgcgggcg accatcctgg ccacgtcgat cgccggcttc tcgaagctga
      841 tgccgtgctg gtcgaagata tggcccatgt tccagaactg gcggctggaa tccagcagcg
      901 ccttggacgg gatgcagccg acgcgcaggc aggtaccgcc gagcgccggc ttgccgtcct
      961 tgcccagcgc ggcatcgatg caggcggtct tcaggcccag ctgggcggcg cggatcgcgg
     1021 cgtggtagcc ggccgggccg gcaccgatca cgacgacatc gaaattctgc acttcactca
     1081 ttgcacttct tccttaacgt tcaaacagga agccccgcat ggcggggctt cgtgcgatca
     1141 catgcccagc agcatccggt gcggattctc gagctggttc ttgatgtcga ccaggaacag
     1201 caccgcgtcc ttgccgtcga taatgcgatg gtcgtagctc agcgcgatgt acatcatcgg
     1261 cgcggcgacg acctggccgt tctcgacgat ggcgcgctcc ttgatggcgt gcatgcccag
     1321 gatcgcgctc tgcggcgggt tgatgatcgg ggtggacatc agcgaaccga aggtgccgcc
     1381 gttggtgatg gtgaaggtgc cgccctgcag gtcgtccagg ccgagcttgc cgtcgcgcgc
     1441 cttcttcgca tacccggcga tcgcgctttc gatgtcggcg aagcccatcc gctcgacgtt
     1501 gcgcagcacc ggcgtgacca ggcccttgtc ggtcgatacc gcgatgctga tatcggcgta
     1561 gccgtggtag atcacgtcgc tgccgtcgac cgaggcattg acgatcgggt ggcgctgcaa
     1621 cgcgttggcg gcggccttgg cgaagaagct catgaagccg agcttgatgc cgttggcctt
     1681 ctcgaacgac tcgcccagct ccttgcgcat cgccatgacc ttgccgaggt tgacctcgtt
     1741 gaacgaggtc agcatcgcga tgctgttctt cgactgcatc aggcgctcgg cgatgcgggt
     1801 gcgcatgcgg gtcatcggca cgcgctcttc cgggcgcgcg ccgccggaca cgccggcggt
     1861 cttgccgctg gcgtaattga cgatgtcttc cttggtgacc atgccgcgac ggccggtgcc
     1921 ttctacctgc gccggatcga cgccggccgt ggtggcggcg aaacgggccc ccggcgggag
     1981 gtcgccagcg gccggtgccg gggtggtgct ggccggagcg gcggcgtgcg ctgcggcggc
     2041 ctgggccttc ggttgcacct catcggcgcc cgcggcgggg gcggccttcg ctgcctcggc
     2101 tgcaggtgcc gcggccacgg caccttcctc gatcaccgcg atcagctgct ggctggtcac
     2161 ggtggcaccg gcctcgaact tgatttcctt gagcacgcca tcgaccggcg agggcacttc
     2221 caggacgacc ttgtcggttt ccaggtcgag caggttctcg tcgcgcttga ccgcgtcgcc
     2281 ggccttcttg tgccaggtgg cgatggtggc gtcggacacg gattcgggga gaaccggaac
     2341 tttgacttcg gtggccatga gggcgtcctg tcgggtatgc gttggaatgg tggtgcgaag
     2401 gggtttattc ggccgtgatc tcggcgccgg ccgggttgac cagggcgtcg gcgaccagct
     2461 tgttctgctc ggccacgtgc tcggccaggt ggccggcggc gggcgagggc gaacgcgggc
     2521 gaccggcgta ggtcagcgtc tgcttgccct gcaggcaagc ctgcaggtgg tgccggatct
     2581 ggtaccacgc gccctggttc tgcggctctt cctggcacca gacgacttca ttggcggagg
     2641 cgaagcgctt gagttcggcg gccagttccg gccgcgggaa cgggtacaac tgctcggcgc
     2701 gaaccagggc gacatcggtg atgccctgct tctgcgcttc gtccagcagg tcgtagtaga
     2761 ccttgccgga acagacgacc acacggcgca ccttcttcga ggtcgcgttc gtgtcgggga
     2821 tcaggcgctg gaactcgcca ttcgccagct cgtcgagcgt agaaaccgcc agcttgtggc
     2881 ggagcaggga cttcggcgtc atcaccacca gcggcttgcg cgtgcccatg cgcatctgcc
     2941 ggcggatcat gtggaaatcc tgcgccggcg tggtcggcgc gcagacgatc atgttctcca
     3001 gcgcgcacaa ctgcaggaaa cgctcaaggc gcgccgagct gtgttccggg ccctggcctt
     3061 cgtaaccatg cggcaggaac agcgccaggc cgcacaggcg gtcccacttg gcttcgccgg
     3121 aggagaggaa ctggtcgatg acgacctggg caccgttggc gaaatcgccg aactggcctt
     3181 cccagatgtc cagcgtcatc gggtcggcgg tcgcgtagcc gtactcgaac gccatcaccg
     3241 cctcctcgct gagcagcgag tcgatgatgg tcacgtcttc cgggctgcgg gccggcgtgt
     3301 ccttcagcaa ttcgcgcagc ggcaggtagt aattgtccgt ggactggtcg tgcaggatcg
     3361 cgtggcgatg gaagaacgtg ccgcggccgc agtcctggcc gaccaggcgc aggcgatagc
     3421 cctcgttcac cagcgtcgcg taggccaggt tctcggcgaa gccccagtcg ccggcctgtt
     3481 cgccggccgc catcttgcgg cggtcctcgt agatcttggc cacgcgcggg tgcagcttga
     3541 ccgcctccgg gattgcgttg atgcgggtcg ccaatgcatc cagggtggcg cgctcgacgc
     3601 gggtatcgac cggatcggac agtttcgccg acaggtacgg actccagtcg atggtgaatt
     3661 cgtcggcctt gacctgcacc acgtcggtgg tgacgtcgcc ggcgtccagc gtattgcgat
     3721 aaccgtcgac cagcgcctgc gacgcggccg ctggcacgct gccttcgcca tccagccgcg
     3781 cggcgtacag ctcgcgggtg gtcttcatcg cgcggatctt ctggtacatc agcggctggg
     3841 tcgcagccgg ctcatcggcc tcgttgtggc cgtggcggcg gtagcagacc aggtcgatga
     3901 ccacgtcctt gccgaagcgc tggcggaaat cgaaggccat ctcggacacg aaggccacgg
     3961 cttccggatc gtcgccgttc acgtgcagga tcggcgcgcc gatcatcttc gccacgtcgg
     4021 tgcagtacag ggtcgaacgc gcatcttccg ccgcgctggt ggtgaagccg acctggttgt
     4081 tgatcaccac gtggacggtg ccgccgacgc ggaagccgcg cgcctgcgac atctggaaca
     4141 gttccatcac cacgccctgg ccggcgaacg
//
LOCUS       DAOOCX010000184         4166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_20200_length_4166_cov_10.171005, whole genome shotgun
            sequence.
ACCESSION   DAOOCX010000184 DAOOCX010000000
VERSION     DAOOCX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20200_length_4166_cov_10.171005"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PK399_11950"
     CDS             <1..309
                     /locus_tag="PK399_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HPW13874.1"
                     /translation="DVFGVHGVGGITGALLTGVFAAPSLGGAGIWDYVTETGVAEYSI
                     GSQVWIQAQGVLVTIVLSGVVALLSYLIVKVTVGLRVSEEAEREGLDITSHGETAYES
                     "
     gene            413..1183
                     /locus_tag="PK399_11955"
     CDS             413..1183
                     /locus_tag="PK399_11955"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPW13875.1"
                     /translation="MKMLRLTFIAATCVLLAACAHAPQAPRNALAEWVPSPNFDARRP
                     VAIVIHATEQGSAAQSLETLRTRNRGGPVSAHYLIGDDGRTYQLVADGDRAWHAGGGR
                     WGTLTDLNSASIGIELDNEVGEAFTEAQLVALLKLLDDLTTRLSIPKAQVIGHADMAP
                     TRKRDPGSLFPWQRLAEAGYGLWPQGELVDPPAGFDPWLAMAAIGYPLNDRAAAMRAF
                     HRHYRGRDDGEDPQAAFDAEDLRILHALSQQRLQPAAR"
     gene            1212..1964
                     /locus_tag="PK399_11960"
     CDS             1212..1964
                     /locus_tag="PK399_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPW13876.1"
                     /translation="MTSTQARHPRFMIACAARTGSTMLVRTLRSHPHLIVHGEVFGDG
                     MVGVDGPLGRECESDPAARDALEAMRFAEPVRALETFLDRHAAHAAGFKLKYDELVRP
                     QWQGVRRLVEADEELAIVFLHRRDLLRRYLSHQVVLRQTGITVVAAGDTPPAVAPFDV
                     DIDDLLRDIAETRRRTREFEAAFALHPSLHVAYEDLAADAQAQCDRVFEFLGVGSALV
                     RVATERIVRTPPEALVLNHDAVKAALRTAGEA"
     gene            complement(1969..2685)
                     /locus_tag="PK399_11965"
     CDS             complement(1969..2685)
                     /locus_tag="PK399_11965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW13877.1"
                     /translation="MRGLLLFVLGALVGANATYFVMTRDAGPPTATRVGERIETPLPT
                     QPATEAGVGAMGADPGSSPTGPMLPPPAVRATGVGPAPTDVPVASMPGAASSLLIPVQ
                     GISVAQLQDTFTDARSGGRVHDAIDIMAPAGTPVLAVADGTVEKLFDSKLGGTTLYQF
                     NPQRTLAYYYAHLQAYAPGIAEQQALKRGQVIGYVGSSGNADPAAPHLHFAIFELGPE
                     QQWWKGTAINPYPRLRGDAR"
     gene            complement(2719..3720)
                     /locus_tag="PK399_11970"
     CDS             complement(2719..3720)
                     /locus_tag="PK399_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPW13878.1"
                     /translation="MKRSLLFVSLLLAGAGATLHAQDPKPVTSSTTTPAAATPQAVAP
                     AEQATAQAGAAASQHLEAQVLLERAWFSPGEIDGEWGSKSRKAVAGFQLARGLAVSGE
                     LDDATWAELRKGGTALVDYTITDADVAGPFAPTPSGMMAKAKLKAIPFQSAAEALGEK
                     FHASPKVLAALNPGVPLDVAGGTLRVPNVLDIAPMPTAAKIEVDKSDAALRLLDASGK
                     AYAQFPITSGTAKFPLPIGDWTIESMTPDPWYNYDPALIVTARKGDRKAKLPPGPNGP
                     VGTMWMALSKPHYGIHGTPEPHLIGRTQSSGCVRLTNWSATAVAAASTVGMTVSMVE"
     gene            3834..>4166
                     /locus_tag="PK399_11975"
     CDS             3834..>4166
                     /locus_tag="PK399_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPW13879.1"
                     /translation="MQDAPTTLDVLDALATPVLQVGGDGRIRLANEAAARWLRVGRKR
                     LLGLPLAALEREGQAVAGALAQSDGNPHLLRRVQLAFPGSNEALFADLWLSPIADGAW
                     CEAHPVDEF"
BASE COUNT          596 a         1384 c         1506 g          680 t
ORIGIN      
        1 gacgtgttcg gcgtgcacgg cgtgggcggc atcaccggtg cgttgctgac cggcgtgttc
       61 gcggcgccgt cgctgggcgg cgcgggcatc tgggactacg tgaccgagac cggcgtggcc
      121 gagtattcga tcggttcgca ggtgtggatc caggcgcagg gcgtgctggt caccatcgtg
      181 ctgtcgggtg ttgtcgcgct gctgtcgtac ctgatcgtca aggtgacggt tggcttgcgc
      241 gtctccgagg aagccgagcg cgaaggcctc gacatcacct cgcatggcga gaccgcgtac
      301 gagtcctgat caccagggga gcccgcaagg gatggtgggc gggcgacggc aacgtcgccc
      361 gcttttttgt ggtgcgcttg ccaattgcag gcgtagccgc tgcaatgccc gcatgaaaat
      421 gctgcgcctg acgttcatcg ccgcgacctg cgtgctgctt gccgcctgcg cccacgcgcc
      481 gcaggcgcca cgcaatgcac tggccgagtg ggtgccgtcg ccgaacttcg atgcgcgccg
      541 gccagtggcg atcgtgatcc acgcgaccga gcagggttcg gccgcacaga gcctggaaac
      601 gctgcgcacg cgcaatcgcg gcggcccggt aagtgcgcac tacctgatcg gcgacgacgg
      661 ccgcacctac cagctggtcg ccgatggcga ccgcgcctgg catgccggcg gcgggcgctg
      721 gggcacgctc accgacctga attctgcctc gatcgggatc gaactcgaca acgaggtcgg
      781 cgaagccttc accgaggcgc aactcgtcgc cttgctgaag ctgctcgacg acctgaccac
      841 gcgcttgtcg atcccgaagg cgcaggtgat cgggcacgcc gacatggcac cgacgcgcaa
      901 gcgcgatccg ggatcgctgt tcccgtggca gcgcctggcc gaggccggtt acggcctgtg
      961 gccgcagggc gagctggtcg atccgccggc gggcttcgac ccgtggctgg cgatggcggc
     1021 gatcggctat ccgttgaacg atcgcgcggc ggcgatgcgc gccttccatc gccactatcg
     1081 cggccgcgac gacggcgaag atccgcaggc cgcgttcgat gccgaagacc tgcgcatcct
     1141 gcacgcgctg tcgcagcagc gactgcagcc ggccgcgcgg tgatcacggt gtgcttgcgg
     1201 gctcgcagcc gatgacatcg acgcaggcgc ggcatccgcg cttcatgatc gcctgcgccg
     1261 cgcgcaccgg cagcaccatg ctggtgcgca cgctgcgcag ccatccgcat ctcatcgtgc
     1321 atggcgaagt cttcggcgat ggcatggtcg gcgttgacgg gccgctgggt cgcgagtgcg
     1381 agtcggaccc ggcagcgcgc gatgccctgg aggcgatgcg tttcgccgaa cccgtgcgcg
     1441 cactggagac gttcctcgat cgccacgctg cccacgccgc cggtttcaag ctgaagtacg
     1501 acgaactggt gcgtccgcaa tggcaggggg ttcgtcgcct cgtggaagcg gatgaggagc
     1561 tcgccatcgt gttcctgcat cgccgcgacc tgctgcgccg ctatctctcg caccaggtgg
     1621 tgttgcggca gaccggcatc accgtggtcg cggctggcga cacgccgccc gcggtcgcac
     1681 cgttcgatgt cgatatcgac gacctcctcc gcgacatcgc cgagacgcgt cggcgcacgc
     1741 gcgaattcga agcggcattc gccctgcatc cgtcgctgca cgtcgcctac gaggacctgg
     1801 cggcggatgc gcaggcacaa tgcgatcgcg tgttcgagtt cctgggggtt ggatcggcac
     1861 tggtgcgcgt cgccaccgaa cgcatcgtgc gcacgccgcc ggaggcgctg gtgctgaacc
     1921 acgacgcggt caaggccgcg ttgcgcacag ccggcgaagc ctgaccaatc agcgcgcgtc
     1981 gccgcgcaac cgcggatacg gattgatcgc ggtgcccttc caccactgct gttccgggcc
     2041 cagctcgaag atcgcgaaat gcaggtgcgg cgcggcgggg tcggcgttgc cggaactgcc
     2101 gacgtaaccg atcacctggc cgcgcttgag cgcttgctgc tccgcgatgc cgggcgcata
     2161 cgcctgcagg tgtgcgtagt agtaggccag cgtccgctgc gggttgaact ggtagagcgt
     2221 ggtgccgccg agcttgctgt cgaacagctt ctcgaccgtg ccgtcggcca ccgccagcac
     2281 cggcgtgccg gccggggcca tgatgtcgat cgcatcatgg acgcgaccac cgctgcgggc
     2341 atcggtgaag gtgtcctgca actgcgcaac gctgatgccc tgcacgggga tgagcaggct
     2401 ggatgccgca ccgggcatgg aggcgacggg aacgtcggtg ggcgccggac ccacgccggt
     2461 ggcgcgtacc gcaggcggcg gcagcatcgg gccggtcggg ctcgaccccg gatcggcgcc
     2521 catcgcgccg acaccggctt cggtggcggg ctgcgtcggc agcggcgttt cgatgcgttc
     2581 gcccacccgc gtcgcggtgg gcgggccggc atcgcgggtc atcacgaaat aggtcgcgtt
     2641 ggcaccgacc agcgcaccga gcacgaacag cagcaatccg cgcaggaagg gacgctggcg
     2701 gcggtcgcgg cgcacggctt actccaccat gctcacggtc atgccgaccg tggaggcagc
     2761 ggccacggcg gtcgcgctcc aattggtcag gcgcacgcag ccgctgctct gggtgcggcc
     2821 gatcaggtgc ggctccggtg tgccgtggat gccgtaatgc ggcttcgaca gggccatcca
     2881 catggtgccg acggggccgt tcgggccggg cgggagcttg gccttgcggt cgcccttgcg
     2941 cgcggtgacg atcagcgcgg gatcgtagtt gtaccaaggg tccggggtca tcgattcgat
     3001 cgtccagtcg ccgatcggca acgggaactt ggcggtaccg ctggtgatcg ggaattgcgc
     3061 ataggccttg ccgctggcat ccagcaggcg cagcgcggcg tcggacttgt cgacctcgat
     3121 cttcgcggcg gtcggcatcg gcgcgatgtc cagcacgttg gggacgcgga gcgtgccgcc
     3181 ggccacatcg agggggacac ccgggttgag cgcggccagc accttcgggc tggcgtggaa
     3241 tttttcgccc agtgcctcgg cggcggactg gaacgggatc gccttcagct tcgccttcgc
     3301 catcatcccc gacggggtgg gcgcgaacgg gccggcgaca tcggcgtcgg tgatcgtgta
     3361 atcgaccagt gcggtgccgc ccttgcgcag ttccgcccag gtcgcgtcat cgagttcgcc
     3421 gctcacggca agcccgcgcg ccagctggaa accggcaacc gccttgcggc tcttgctgcc
     3481 ccattcgccg tcgatctcgc cgggcgagaa ccacgcgcgc tccagcagga cttgcgcttc
     3541 caggtgctgc gatgccgccg cgcctgcctg cgccgtcgct tgctccgcgg gtgcgacggc
     3601 ttgcggcgtg gccgctgcgg gcgtcgtcgt cgacgaagtg accggctttg gatcctgtgc
     3661 atgcagcgtg gcaccggcac cggcgagcag gagcgagaca aacaagagcg atcgtttcat
     3721 ggggtcacct gaggaatgcc catgcaggct gcgggagtcg ggatcgccgt cgcgtgaatg
     3781 cgcgtcggtc gtcggggtgc gcattcaggc gggctttgtt ctagattcca ccgatgcagg
     3841 acgcgcccac gaccctcgat gtgctggatg ccttggccac cccggtcttg caggtgggcg
     3901 gcgacggacg catccgcctg gccaacgagg ccgctgcgcg ctggctgcgg gtgggccgca
     3961 agcggttgct cggcttgccg ctggcggcgc tggaacgcga gggacaggcg gtcgccggcg
     4021 cgctggcgca gagcgacggc aatccgcacc tgctgcgccg cgtgcagctg gctttcccgg
     4081 gcagcaacga ggcgctgttc gccgacctgt ggctgagccc gattgcggac ggtgcctggt
     4141 gcgaggcgca tccggtcgac gaattc
//
LOCUS       DAOOCX010000185         4166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_20201_length_4166_cov_6.979324, whole genome shotgun sequence.
ACCESSION   DAOOCX010000185 DAOOCX010000000
VERSION     DAOOCX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20201_length_4166_cov_6.979324"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PK399_11980"
     CDS             complement(<1..1205)
                     /locus_tag="PK399_11980"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW13880.1"
                     /translation="MSKKDESTRRPLSLKAKAAPETTAAPRIEERLHKVLAQAGLGSR
                     RALEQRIADGLVKVNGEVARTGMSITGGDKIELDGKTFVASALVDAPRVLIYNKPESE
                     VTTREDPEGRPTIFEALPTLKGARWIAIGRLDINTTGLLLLTTDGELANAMMHPSFEV
                     EREYVCRVRAPEGEDVVNEKIVDRLARGVALDDGPAKFDEIERIGGTDSHDWFRVLLK
                     EGRNREVRRLWESQGCQVSRLKRIRYGQVRLPQPLLRGQSQELPQAEVDALRKSVGLE
                     DGTPSALTLMPVLGQRKASKTVVTGNARSHGYVGGHSTADEGRELRRFDNVREDRGRG
                     RGAPRKPGGLTVSGEAAAKQANKTFKPKGPRPQGQRLRPGEQRGENPTEMRTWYVPDG
                     VDTGPSGHR"
     gene            complement(1235..2038)
                     /gene="scpB"
                     /locus_tag="PK399_11985"
                     /pseudo
     CDS             complement(1235..2038)
                     /gene="scpB"
                     /locus_tag="PK399_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017165259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            complement(2141..3055)
                     /locus_tag="PK399_11990"
     CDS             complement(2141..3055)
                     /locus_tag="PK399_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HPW13881.1"
                     /translation="MTSEPSTGASADLHANPNARPQQQEMPLAMVLGQPVLQIPQDLY
                     IPPDALEVILDAFEGPLDLLLYLIRRQNLDILDIPVASITRQYVDYIQGMHELRFELA
                     AEYLVMAAILAEIKSRLLLPRPPSEEGIEEDPRADLVRRLQEYERYKKAAEDLDTLPR
                     QERDTAPAHAFVPDRAEIRLPPPVDIREMLLALHDVFKRAELYTQHAIKRDALSVRQR
                     MGELLERLADGQFHRFESLFTVEEGKLGVVVTFLGLLTLAKEQLLDIVQEGPTAPIYV
                     KSLASDEGAPAVTDLSSEFDDEPAANDE"
     gene            3351..3650
                     /locus_tag="PK399_11995"
     CDS             3351..3650
                     /locus_tag="PK399_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPW13882.1"
                     /translation="MWYAIEGFDGTDVLPKRMAARPEHLARLVSLRDEGRLLLAGPCP
                     AIDAEDPGPAGFSGSIVIAEFASLEDARAWADADPYVAAGVYARVEVRPFRKVLP"
     gene            3647..3958
                     /locus_tag="PK399_12000"
     CDS             3647..3958
                     /locus_tag="PK399_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HPW13883.1"
                     /translation="MTTGWTEADLPRLNPQRVERIRALLTEALAPQSLEVADDSHKHA
                     GHAGARGGQGHFGVDIVSSAFSGKLPLARHRLVYAALGEMIQTDIHALSIRARTPDEA
                     G"
BASE COUNT          548 a         1376 c         1487 g          755 t
ORIGIN      
        1 ttgcgatggc cgctggggcc ggtgtccacg ccgtccggca cgtaccacgt gcgcatctcg
       61 gtgggattct cgccgcgctg ctcgcccggg cgcaggcgct ggccctgcgg gcgcggcccc
      121 ttcggcttga aggtcttgtt ggcctgcttg gcggcggctt cgccgctgac ggtcaggccg
      181 cccggcttgc gcggggcgcc acggccgcgg ccgcggtcct cgcgcacgtt gtcgaagcgg
      241 cgcagttcgc ggccttcgtc ggcggtgctg tggccgccca cgtagccgtg gctgcgggca
      301 ttcccggtca ccacggtctt gctggccttg cgctggccca ggaccggcat gagggtgagc
      361 gcggacggcg tgccgtcctc cagcccgacc gacttgcgca gcgcatcgac ctcggcctgc
      421 ggcagttcct gcgactggcc gcgcagcagc ggctgcggca ggcgcacctg gccgtagcgg
      481 atgcgcttca ggcgcgagac ctggcagccc tgcgattccc acaggcggcg cacttcgcgg
      541 ttgcggcctt ccttcagcag cacgcggaac cagtcgtgcg aatcggtgcc gccgatgcgt
      601 tcgatctcgt cgaacttggc cgggccgtcg tccagcgcca cgccgcgggc caggcggtcg
      661 acgatcttct cgttgaccac gtcctcgcct tcgggcgcgc gcacgcggca gacgtattcg
      721 cgttcgacct cgaagctcgg gtgcatcatc gcgttggcca gctcgccgtc ggtggtgagc
      781 aacagcaggc cggtggtgtt gatgtcgagg cggccgatcg cgatccagcg cgcgcccttc
      841 agcgtgggca gcgcctcgaa gatggtcggc cggccttccg ggtcttcgcg ggtggtgacc
      901 tcgctttccg gcttgttgta gatcagcacg cgcggcgcat cgaccagcgc gctcgcgaca
      961 aaggtcttgc cgtccagctc gatcttgtcg ccaccggtga tgctcatgcc ggtgcgcgcg
     1021 acctcgccgt tgaccttgac caggccgtcg gcgatgcgct gctccagcgc gcgccgcgag
     1081 cccaggccgg cctgcgccag caccttgtgc aggcgctcct cgatgcgcgg tgcagcggtg
     1141 gtttccggcg cggcctttgc cttcaggctc agcgggcggc gggtggattc gtctttcttg
     1201 ctcattgttt ctgctccgac gatgcgggat tcgcgtcgtc tccggggtgg tcggtgtcgg
     1261 tttcgatgtc gccgatggat tcggcgctat cggtttcgat gtcggtgtcg gcaatggcgg
     1321 gcgaggtggt gtcctcgtcc gagccattgc tgtcggacat ggcatcggcc gccatgtctt
     1381 cgtggtcttg ctcgtgttcg atgtcgctgt cttcgccggg catggtctcc aaccccgttg
     1441 cggccgaggt cgaggccgaa gccgcgccgg ccgggatcgc cgccgcgaac ggcaattgcg
     1501 gctccagctc gccgagatcg cgcaattcgg acagcggcgg cagttcgtcc agccgcttca
     1561 ggccgaagta gtcgaggaag gtcttggtgg tcgccagcag ttccggcttg ccgggcacgt
     1621 cgcggtggcc gaccacgcgg atccactcgc gttcctccag cgccttgatg atgctgctgt
     1681 tgaccgccac gccgcggacc tgctcgatct cgccgcgggt gatcggctgg cgataggcga
     1741 tcagcgccag cgtctccagg gtggcgcggg tgtacttggt ctggcgctcg gtccacaggc
     1801 gggccaccca cgggtggaca tcggcctgga cctggaagcg ccagccgctg gccacttcga
     1861 ccagttccac gccgcgttcc gcgcaggcgt cctgcaaggc ctgcagggcg gcctggatgc
     1921 tgccttccgg ggctggctcg tccagcggga acagggcatt gagctggacc acggtcaatg
     1981 gctggctggc cgcgagcagg gcggcttcga cgatgcgggt aacgagggtt tgatccatgt
     2041 gtctctgcga tgtcatcccg ggcacgaggc gagggatctg tttgtcatgg ggcggtgtcg
     2101 atgttggaac aggtccctcg cgtggctcgg gacatcacgc tcattcgtcg ttggccgccg
     2161 gctcgtcatc gaactcgctc gacaggtcgg tgacggcggg agcgccctcg tcggaggcga
     2221 gcgacttcac gtagatcggt gcggttggtc cttcctggac gatgtcgagc aactgttcct
     2281 tggccagggt gagcaggccg aggaaggtca ccaccacgcc gagcttgcct tcctcgacgg
     2341 tgaacagcga ttcgaagcgg tggaactggc catcggcgag gcgctccagc agttcgccca
     2401 tgcgctggcg gacgctgagc gcatcgcgct tgatcgcgtg ctgggtgtac agctcggcgc
     2461 gcttgaacac gtcgtgcagg gccagcagca tctcgcggat gtccaccggc ggcggcaggc
     2521 ggatctccgc gcgatcgggg acgaaggcat gcgccggggc ggtgtcgcgt tcctgccggg
     2581 gcagggtgtc aaggtcttcg gccgccttct tgtagcgttc gtactcctgc aggcggcgga
     2641 ccaggtcggc acgcgggtct tcctcgatgc cttcctcgct gggcgggcgc ggcagcagca
     2701 ggcgcgactt gatctcggcc aggatcgcgg ccatcaccag gtattcggcg gccagctcga
     2761 aacgcagttc gtgcatgccc tggatgtagt cgacgtactg gcgggtgatc gaggccaccg
     2821 ggatgtcgag gatgtccagg ttctgccggc ggatcaggta cagcaacaag tcgagcgggc
     2881 cctcgaaggc gtcgaggatg acttccagcg cgtccggcgg gatgtacagg tcctgcggga
     2941 tctgcagcac cggctggcca agcaccatcg ccagcggcat ttcctgctgc tgcggtcggg
     3001 cattcgggtt ggcgtgcaag tcggcgcttg cgccggtact cggttcggat gtcatcggtt
     3061 ggcggccccc tccgggccac accttcgtcg catgaggccg caagcggccg ggcagtgctg
     3121 cgtggagctg catcccgcca tgcctgcgcg cgctggcgcc cggttggggc ggcggcggga
     3181 cgggtggcgg agaggccgga ttggcgggcg cggtgcggcc tggcggggcc ggaagtcgcg
     3241 cggggccgca aggtcgggcc tgaaacgagg ccgcagggta agcgctgcag cgggccgtgt
     3301 ccactcgccc cctctacaat gcccgcccgg gttcatggag cgtcatgccg atgtggtacg
     3361 cgatcgaagg gttcgacggc accgatgtgc tgcccaagcg gatggccgcg cggcccgagc
     3421 acctggcgcg attggtctcg ctgcgcgatg aaggacgcct gctgctggcc ggcccttgcc
     3481 cggccatcga tgccgaggat ccgggcccgg cgggcttcag tggcagcatc gtcatcgccg
     3541 aattcgcctc gctggaggac gcccgcgcct gggcggacgc cgacccgtac gtcgccgccg
     3601 gcgtgtacgc gcgggtcgag gtgcgaccgt tccgcaaggt cctgccatga ccaccggttg
     3661 gaccgaggca gacctgccgc ggctcaaccc gcagcgggtc gaacgcatcc gcgcgttgct
     3721 gaccgaggcg ctggccccgc aatcgctcga ggtcgccgac gacagccaca agcacgcagg
     3781 gcacgccggc gcgcgcggcg ggcaggggca tttcggcgtc gacatcgtca gcagcgcgtt
     3841 ctccggcaag ctgccgctgg cccggcatcg gctggtctat gccgcgctgg gcgagatgat
     3901 ccagaccgac atccatgcgc tctcgatccg ggcccgcacg ccggacgagg cgggctgacc
     3961 cgctatactc cttgatcgcg ctgcatttgc ggccaagctc cgttaattaa atcaattggt
     4021 tagcggtaaa agttaatccc tgatatcagg cagcggctgg ccgctgtccg taggtcgcca
     4081 ttgccgcatg cgcctgtcca ccatcaagct gtccggcttc aaatccttcg tcgatccgac
     4141 caccctgcac ctgccgacca acatga
//
LOCUS       DAOOCX010000186         4150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_20305_length_4150_cov_6.020513, whole genome shotgun sequence.
ACCESSION   DAOOCX010000186 DAOOCX010000000
VERSION     DAOOCX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20305_length_4150_cov_6.020513"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..432
                     /locus_tag="PK399_12005"
     CDS             49..432
                     /locus_tag="PK399_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13884.1"
                     /translation="MHRTRLSIIAALAALLLAGCGKDAAPGTSGAVGEDGLPKPDTVS
                     GSVTGMPNPGESTPNPGQPVDVPPDEDPDTVEAGAPVDGELPAEVDGMPQTATPEPPE
                     PESMPMPPPESATPANGGTPAAGQP"
     gene            429..1148
                     /locus_tag="PK399_12010"
     CDS             429..1148
                     /locus_tag="PK399_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13885.1"
                     /translation="MKQALLAASVAMLVACSPAPAPTPAPPAAKPAPVAVPAAETPAP
                     VATEAAAPVVESWELPGDLGPLTTQAQLEARFGKANLREETFDGAEGIGTYPVLVAFP
                     DDPAKRLELLLDADNKDAPIQELRVSNPDSQWHDATGLRPGMTLGELVKLNGAPVSFY
                     GLAWDYGGTVQDWHGGKLANAVGNPLFRRVTLAAREGADDNSLPQGDATFRSDDPKWT
                     NAGKDLVVGELGISWPHEGED"
     gene            complement(1158..>2443)
                     /locus_tag="PK399_12015"
     CDS             complement(1158..>2443)
                     /locus_tag="PK399_12015"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPW13886.1"
                     /translation="ITGAIKFNGSIGATQYGAFLADEGGDGGRRFSALRLQRAFGAHN
                     LGAMLTHVDRPFLDRSANVLGFDHRWQPDASLTVSSTLVGSDIEQAGQHVRDFAFTSM
                     AQKTLDKGWSLTGLLIHYGEDFEINDAGYLGRNDLNYGQFEVGKRITDLPADSAWSSH
                     NLRFRIEGMQNNDRLWLQRQFRFNGNSQRRGGGNLNWNLQLRTAAFDDEITRGHGPMR
                     VRNGGNAFIGRNFPRRGNWEFGVEGSIGVASGLRQDTPYAWSASASSTYYINDALNIE
                     LWLGHVMDQEQLIWQGRDLGPGYENVVGSFHMNRYDLSASLNWNIGTRQELRVKLEAL
                     GFDADDPIAWRLGADGRGVASNDPVQPFSLRNMGFQVRYRYELAPLSNLYVVYGRGGF
                     GYDPYAAGAFDQFNDAFSLRNDEQLVVKLAYRFEL"
     assembly_gap    2444..2543
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2544..3583)
                     /locus_tag="PK399_12020"
     CDS             complement(<2544..3583)
                     /locus_tag="PK399_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPW13887.1"
                     /translation="MRLSRHKTSLLSLAVLTALSSPVLAQVKVDGAIDPAEWQGARHV
                     SDFRMVQPFTQSATRHPTEAWILATPDGLAVAFRNTKIAGVETPRQRSARDQDVAIDR
                     VNVMLDFDGDGRSGYDFTLTASDSIQDSTITGGGNFSSDWDGSWQHAVVDGEGEWTAE
                     LLIPWHTAPMKKATGDKRTVAVYLDRVVGSVGERMAWPAVTFERPQFLNQFEKIEIPV
                     YSQSLLAITPYVVGLHDLVSRDNQFDAGADIFWKPNSQFQLTATINPDFGQVESDSLV
                     VNFGAQETFFSDKRPFFTENQGFFDFGLLFDNSQLLYTRRIGSHADDHSGPADITGAI
                     KFNGSIGATQYGA"
     gene            complement(3731..>4150)
                     /locus_tag="PK399_12025"
     CDS             complement(3731..>4150)
                     /locus_tag="PK399_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HPW13888.1"
                     /translation="GMFAVAQIAKGERLIEYKGRRRTHEEVDAGDTGDAESGHTFLFT
                     LNEVFVIDANFEGNSARWINHSCAPNCEAVLEEHEDDPEKSRVFIDAKRAIKPGEELT
                     YDYGITLAERHTERLKKIWACRCGAKDCTGTMLRPKR"
BASE COUNT          658 a         1359 c         1345 g          688 t
ORIGIN      
        1 aacgggtcga catcgatccc ggcctcatca ccatccgact ggactgacat gcatcgcacc
       61 cgcctttcga tcatcgccgc gctcgccgcc ctgctgctcg ccggttgcgg caaggacgca
      121 gccccgggca ccagcggcgc cgtcggggaa gacggcctgc cgaaaccgga taccgtcagc
      181 ggttcggtca ccgggatgcc caaccccggc gagtccacgc ccaatccggg ccaaccggtc
      241 gatgtgccgc cggacgagga cccggacacc gtggaggcag gcgcgccggt cgatggcgaa
      301 ctgccggcgg aggtcgacgg catgccgcag actgccaccc ccgagccgcc ggaacccgag
      361 tcgatgccga tgccgccgcc ggagtccgcg acgcccgcca acggcggcac accggcagca
      421 ggccagccat gaagcaggcg ctgctcgctg ccagcgtggc gatgctggtg gcctgttcgc
      481 ccgcacccgc gccgacaccg gcaccgcctg ccgcgaagcc tgcgcccgtc gccgtgcccg
      541 cggccgagac gcccgcgccc gtggcgactg aagccgcggc accggtcgtc gaaagctggg
      601 aactgccagg cgacctcggc ccgctcacca cgcaggcgca actggaagcg cgcttcggca
      661 aggccaacct gcgcgaggaa accttcgacg gcgccgaagg catagggacg tatccggtgc
      721 tggtggcgtt tcccgacgat cccgcgaagc gtctcgagct actgctcgat gccgacaaca
      781 aggacgcacc gatccaggaa ctgcgcgtca gcaatccgga ttcgcagtgg catgacgcca
      841 ccggcctgcg tccgggcatg acgctcggcg aactggtcaa gctcaacggc gcgccggtga
      901 gtttctacgg cctggcctgg gattacggcg gcaccgtgca ggactggcat ggcggcaagc
      961 tcgccaatgc ggtcggcaac ccgctgttcc gccgcgtcac cctggccgcc cgcgagggcg
     1021 ccgatgacaa ctccctgccg cagggcgacg ccaccttccg cagcgacgat ccgaagtgga
     1081 ccaatgccgg caaggacctg gtcgtcggcg agctcggcat cagctggccg cacgaaggcg
     1141 aggattgacc gccccgctta caactcgaag cgataggcca gcttgactac cagctgttcg
     1201 tcgttgcgca ggctgaaggc atcgttgaac tggtcgaagg cgcctgcggc atacgggtcg
     1261 taaccgaagc cgccgcgtcc gtagacgaca tacaggttcg acagcggcgc gagctcgtag
     1321 cggtagcgca cctggaaccc catgttgcgc aggctgaagg gctgcaccgg atcgttgctg
     1381 gccacgccgc gaccgtccgc gccgagccgc cacgcgatgg ggtcatcggc atcgaagccc
     1441 agcgcttcca gcttcacccg caattcctgg cgcgtgccga tgttccagtt caggctggcg
     1501 ctgaggtcgt agcggttcat gtggaagctg cccacgacat tctcgtaacc gggcccgagg
     1561 tcgcggcctt gccagatcag ctgctcctgg tccatcacgt ggcccaacca caactcgatg
     1621 ttgagcgcat cgttgatgta gtacgtgctg ctggccgagg cactccacgc atacggcgtg
     1681 tcctggcgca ggccgctggc cacgccgatg ctgccctcca cgccgaactc ccagttgccg
     1741 cggcgcggga agttgcggcc gatgaaggca ttgccgccgt tgcgcacgcg catcggcccg
     1801 tggccgcggg tgatctcatc gtcgaaggcg gcggtgcgca gttgcaggtt ccagttgagg
     1861 ttgccgccgc cacggcgctg gctgttgccg ttgaagcgga actgccgctg cagccatagc
     1921 ctgtcgttgt tctgcatgcc ctcgatgcgg aagcgcaggt tgtgcgatga ccacgccgaa
     1981 tccgccggca ggtcggtgat gcgcttgccc acttcgaact gcccgtagtt gaggtcgttg
     2041 cggcccaggt agcccgcatc gttgatctcg aaatcctcgc cgtaatggat cagcaggccg
     2101 gtgaggctcc agcccttgtc cagggtcttc tgcgccatgc tggtgaaggc gaagtcgcgg
     2161 acgtgctggc cggcctgttc gatgtcgctg ccgaccagcg tgctcgagac cgtcaagctg
     2221 gcgtcaggct gccaacgatg atcgaagccc aggacattgg cgctgcgatc caggaacggt
     2281 cgatcgacat gggtcagcat cgcaccgagg ttgtgcgcgc cgaacgcacg ctgcaggcgc
     2341 agggcgctga agcggcggcc gccatcgccg ccctcgtcgg ccaggaaagc accgtactgg
     2401 gtggcgccga tgctgccgtt gaacttgatc gcgccggtga tgtnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnngcaccgt actgggtggc gccgatgctg ccgttgaact
     2581 tgatcgcgcc ggtgatgtcc gccgggccgc tgtggtcgtc cgcatgcgag ccgatgcgcc
     2641 gcgtgtacag caactggctg ttgtcgaaca gcaggccgaa atcgaagaag ccctggttct
     2701 cggtgaagaa cggccgcttg tcgctgaaga aggtttcctg cgcgccgaaa ttcaccacca
     2761 gcgaatcgct ctcgacctgg ccgaagtccg ggttgatcgt ggcggtgagc tggaactggc
     2821 tgttcggctt ccagaagatg tccgcgccgg cgtcgaactg gttgtcgcgg gagacgaggt
     2881 catgcaggcc gaccacgtaa ggcgtaatcg cgagcaagga ctgcgagtaa accgggatct
     2941 cgatcttctc gaactggttg aggaactgcg ggcgctcgaa ggtgactgcc ggccatgcca
     3001 tgcgctcgcc gacgctgccc accacgcggt ccaggtagac cgccacggtg cgcttgtcac
     3061 ccgtggcctt cttcatcggc gcggtatgcc acgggatcag caattccgcg gtccattcgc
     3121 cctcgccatc gaccacggcg tgctgccagc tgccgtccca gtcgctgctg aagttgccgc
     3181 ccccggtgat ggtcgagtcc tggatgctgt cgctggcggt cagggtgaag tcgtagccgc
     3241 tgcggccatc gccgtcgaag tccagcatca cgttgacccg gtcgatcgcc acgtcctggt
     3301 cgcgcgcgct gcgctggcgc ggggtttcca cgccggcgat cttggtgttg cggaaggcga
     3361 ccgccagccc gtcaggagtg gccaggatcc aggcttccgt cgggtggcgg gtcgcggact
     3421 gggtgaacgg ctgcaccatc ctgaagtcgg agacgtggcg cgcgccctgc cattcggcgg
     3481 gatcgatcgc gccatccacc ttgacttgcg caagtacggg cgatgacaac gccgtgagca
     3541 cggcaagcga aagcagggat gtcttgtggc gggacaggcg catcggcggc gtcgaagggc
     3601 agccattgct gcgatgacgc gcaccctaac gattccgtta accggcaccg actggcacaa
     3661 gtcatgtaaa ccttcgtcat gtgcctggca tcctgcccca acccgtggcc gccatgccgc
     3721 cggtcttgcg tcagcgcttg ggccgcagca tcgtcccggt gcagtccttc gcgccgcagc
     3781 ggcaggccca gattttcttc aggcgctcgg tatggcgctc ggccagggtg atgccgtagt
     3841 cgtaggtcag ttcctcgccc ggcttgatcg cgcgcttcgc atcgatgaac acccgcgact
     3901 tttccggatc gtcctcatgc tcttccagca ccgcctcgca gttcggcgca cagctgtggt
     3961 tgatccagcg cgcgctgttg ccctcgaagt tggcgtcgat gacgaacacc tcattgaggg
     4021 tgaacaggaa ggtgtggccg ctttcggcgt cgccggtgtc gccggcatcg acctcttcat
     4081 gggtgcggcg gcggcccttg tactcgatca ggcgctcgcc cttggcgatc tgcgcgacgg
     4141 cgaacatgcc
//
LOCUS       DAOOCX010000187         4126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_20438_length_4126_cov_8.573815, whole genome shotgun sequence.
ACCESSION   DAOOCX010000187 DAOOCX010000000
VERSION     DAOOCX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20438_length_4126_cov_8.573815"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PK399_12030"
     CDS             <1..345
                     /locus_tag="PK399_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW13889.1"
                     /translation="WDAHRDTVAKLMIDTVNDYAPNFARSVIGYEALSPLDLERRIGL
                     VGGDIFHGALGLDQMFSARPLLGQGNYRGAFKQLYLCGSGTHPGGGVTGLPGRNAARE
                     ILKDLRKGPRPD"
     gene            complement(462..2522)
                     /locus_tag="PK399_12035"
     CDS             complement(462..2522)
                     /locus_tag="PK399_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW13890.1"
                     /translation="MTSFRHLPASRWIGIVLVLALALLADLRSAAGTRLDSFTVDEPW
                     HVVAGTAYVRGEGFHLNPEHPPLVKLWVGAWMPETFKLPPMPALHEKSQERDWVETTM
                     FFGNDARAVQTRARLALWTLHGLMLVALGLLLWRAFGLAWACGTLLFLALEPTVGAHL
                     PVVMTDLPLALSLAIAALCAGLLATTWRWPWAIGCGLAMGVALASKHSALAGLGGLGI
                     GLVIAALCGWRQGRGEIARRMAQLAAAAALSVLLLWSMYGLHFHAGADGRDAFNRPIA
                     DKIDELKLPHWRNAIAFADDHRLLPRAYLWGLADTVRTGVEGRGIGMHQVWGTTWYGN
                     PPWFSWPAILASKLPLALMALLLAALPLLARTRLPPSARWSLAAVVAACGFHLVALMG
                     SEGIWGGVRHALPILVGMAVIAGGAFGIAMQRRSRALLIGVTALYLLAAGMTLREPRL
                     WEYHNELVGGSANAYRYFGNEGLDLGQRFHEIRAFHDRVIAPSGEPMYNEYWMGEKQV
                     RAAKLNFHRRVESLDDTNVAGRYKGWFIYTMTDTLPWPQWDWDPAVVFKDMRLVARFG
                     YVGIWHGEMTRPQTRAGSMFSRISDYIYKDGGDDWKLVSDRLEEVVAQLPAKVDAGVE
                     LGNAYLRQGRRSEAIAAYRGLLTQTKVPVEPSIAAQLKAQIAAIEAAPKDATVEPMRN
                     PWLE"
     gene            2626..>4126
                     /locus_tag="PK399_12040"
     CDS             2626..>4126
                     /locus_tag="PK399_12040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW13891.1"
                     /translation="MGRTPLFRLLQRAAAIARASRHVRMPLDEFHDMVRTQRFDRRRR
                     RLLQGAGASALLSGCSSVPNPMRAGTDDEVVIVGAGIAGLTSAWRLRQQGVRVRVFEA
                     QERIGGRMLSLRNHFADDQVIELGGELIDTGHARIRALAGELGIALDDLLDGDGDHDT
                     WFFDGRAIGEAELVRAFVPVAAAIERDLASAGDGSYDHQDSNPAFRALDAMSITQWFD
                     RNGVSGWLRKLLDVAYTTEMGLEIDQQSALNFLTFVGTEDKDAFRIFGESDERFHVRG
                     GNDLIPRTLAAKMTDAIETGHVLEAIRGEAGGYVLAFRKGAATREVRARQVILALPFT
                     LLRKVQVDVELPALKKHAIAQLTYGTNAKLMIGFERRTWREHGANGASMSDLAYQTTW
                     DTSRKQAGTAGALTNFTGGRHGVGLGQGTPKQQAEAAVRDLERVFPGIAAARGNAREA
                     RMHWPTNPWVLGSYACFRPGDWAGLGGVMGESVGGLHFAGEHCSVESQGF"
BASE COUNT          698 a         1428 c         1377 g          623 t
ORIGIN      
        1 tgggacgcgc accgcgacac cgtggcgaaa ctgatgatcg atacggtcaa cgactacgca
       61 ccgaacttcg cgcgcagcgt gatcggctac gaggcgttga gcccgctcga cctcgagcgt
      121 cgcatcggcc tggtcggcgg tgacattttc cacggtgcgt tggggctgga ccagatgttc
      181 agcgcgcgtc cgttactcgg ccagggcaat tatcgcggtg cgttcaagca gttgtacctg
      241 tgcggttccg gcacccatcc cggtggcggc gtgaccggct tgccgggccg caatgcggcg
      301 cgcgaaatcc tgaaggatct tcgcaaggga ccgcggccgg actgaagcct gtccgggctc
      361 ggcgtcggtc gcggccggca ttcgcttcgc ggctcgaaat gccgctgcgc gaacaccggc
      421 cacacccacc gcctacagta agttccccgg cgatgccgag gtcattccag ccacggattg
      481 cgcatcggtt cgaccgttgc atccttcggc gcagcttcga tcgcggcgat ctgcgccttc
      541 aactgcgcgg cgatggacgg ctcgaccggc accttggtct gcgtcaatag cccgcgataa
      601 gcggcgatcg cttccgagcg ccggccttgc cgcaggtacg cattgccgag ttcgacaccg
      661 gcatcgacct tggccggcaa ctgtgcgacg acttcttcca gccgatccga caccagtttc
      721 cagtcgtcgc cgccgtcctt gtagatgtag tcgctgatcc tggaaaacat gctgcctgcg
      781 cgcgtctgcg ggcgggtcat ctcgccatgc cagatgccca cgtagccgaa gcgcgcgacc
      841 agtctcatgt ccttgaatac gacggctgga tcccagtccc actgcggcca cggcaacgtg
      901 tcggtcatcg tgtaaatgaa ccagcccttg taacgaccgg cgacgttggt gtcgtccagc
      961 gattccaccc gcctgtggaa gttgagcttc gccgcgcgta cctgcttttc ccccatccag
     1021 tattcgttgt acatcggctc gccggagggc gcgatcacgc ggtcgtggaa ggcgcggatc
     1081 tcgtggaaac gctggcccag gtcgaggcct tcgttgccga aatagcgata ggcattggcg
     1141 ctgccaccga ccagttcgtt gtggtactcc cacagccgtg gctcgcgcag ggtcatgcct
     1201 gcggccagca ggtaaagcgc ggtcacgccg atcagcaagg cacgcgaacg ccgttgcatg
     1261 gcgatgccga atgcgccgcc ggcgatcacc gccattccaa ccaggatcgg cagcgcatgc
     1321 cgcacgccgc cccagatgcc ttccgagccc atcagcgcga ccaggtggaa gccgcaggcc
     1381 gcgaccacgg cggccaggct ccagcgtgcg ctgggcggca atcgcgtgcg cgccagcagc
     1441 ggcaacgcgg ccagcaacaa ggccatcaac gcgagcggca gtttcgacgc caggatcgcc
     1501 ggccagctga accacggcgg gttgccgtac caggtcgtgc cccagacctg gtgcatgccg
     1561 atgccgcgac cttccacgcc ggtgcggacg gtatcggcca gcccccacag gtaggcgcgc
     1621 ggcagcagcc ggtggtcgtc ggcgaaggcg atcgcgttgc gccagtgcgg caatttcagt
     1681 tcatcgatct tgtccgcgat cgggcggttg aaggcatccc ggccatcggc gccggcatgg
     1741 aagtgcaggc catacatcga ccacagcaac aggaccgaca acgccgccgc cgccgcgagt
     1801 tgcgccatcc gccgcgcgat ctcgccacgc ccctgccgcc agccgcacag cgcggcgatc
     1861 accaggccga tcccgagacc tcccaggccg gcaagcgcag aatgtttcga ggccaaggcc
     1921 acccccatcg ccagcccgca gccgatcgcc catggccagc gccacgtggt ggccagcagg
     1981 ccggcgcaga gcgcggcgat ggccagcgac agcgcgagcg ggaggtcggt catcaccacc
     2041 ggcaggtgcg caccgaccgt cggttccagc gccaggaaca gcaaggtgcc gcacgcccag
     2101 gccaggccga acgcacgcca cagcagcaga cccagcgcca ccagcatcag cccgtgcagt
     2161 gtccacaacg ccagccgcgc gcgggtttgc accgcgcgcg cgtcgttgcc gaagaacatc
     2221 gtggtctcga cccagtcgcg ctcctgcgac ttctcgtgca gcgcgggcat cggcggcagc
     2281 ttgaacgtct ccggcatcca ggccccgacc cacagcttca ccagcggcgg atgctcggga
     2341 ttgaggtgga agccctcgcc gcggacgtag gcggtgcctg ccaccacatg ccacggctcg
     2401 tccacggtga aactgtccag gcgcgtcccc gcggccgaac gcaggtcggc gagcaaggca
     2461 agcgcaagca ccagcacgat gccgatccag cgcgaagctg ggaggtggcg gaaggacgtc
     2521 atgccgttgg ctaacgcggg ccgcgccatg cgccggacac ccgcgagtgt cccgtcgcgc
     2581 gcccgcgtcg cgtattcccg gtgaaaggga cacggaaacc acgccatggg acgcacgccg
     2641 ctcttccgcc tgctgcaacg cgccgccgcc atcgcccgcg catcgcgcca tgtgcgcatg
     2701 ccgctggacg aattccacga catggtgcgg acccagcgct tcgatcgacg ccggcggcgc
     2761 ctcctgcagg gcgcaggcgc cagcgcgctg ctgtcaggtt gcagctccgt gccgaacccg
     2821 atgcgcgcgg ggacggatga cgaagtggtc atcgtcggtg ccggcatcgc cgggctcacc
     2881 tcggcgtggc gtctgcgcca gcagggcgtg cgcgtgcgcg tattcgaggc gcaggaacgc
     2941 atcggcggac gcatgctctc gttgcgtaac catttcgccg acgaccaggt gatcgaactc
     3001 ggcggcgaac tgatcgatac cggccatgcc cgcatccgcg cgctggccgg cgaactcggg
     3061 attgcgctgg atgacctgct cgacggcgac ggcgaccatg acacctggtt cttcgatggt
     3121 cgcgcgatcg gcgaggcgga actcgtgcgc gcgttcgtgc cggtcgctgc ggcgatcgag
     3181 cgcgacctgg catccgccgg cgatggcagc tacgaccacc aggacagcaa tccggcattc
     3241 cgcgcgctgg atgcgatgag catcacccag tggttcgacc gcaacggcgt gtccggctgg
     3301 ctgcgcaagt tgctggacgt ggcctacacc accgagatgg gactggagat cgaccagcag
     3361 tccgcgctga atttcctgac cttcgttggc accgaggaca aggatgcctt caggatcttc
     3421 ggcgaaagcg acgagcgctt ccatgtgcgc ggcggcaacg acctgatccc gcgcacgctg
     3481 gcggcaaaga tgaccgatgc gatcgagacc ggccacgtac tggaagcgat tcgcggcgag
     3541 gcaggcggct acgtgctcgc cttccgcaag ggcgcggcca cccgcgaggt acgtgcgcgg
     3601 caggtgatcc tggccttgcc gttcaccctg ctgcgcaagg tgcaggtcga tgtcgaactg
     3661 ccggcgctga agaagcacgc catcgcgcaa ctcacctacg gcaccaatgc caagctgatg
     3721 atcggcttcg agcgccgcac ctggcgcgag cacggcgcga atggcgcgtc gatgagcgac
     3781 ctggcctacc agaccacgtg ggatacctcg cgcaagcagg ccggcaccgc gggtgcgctg
     3841 accaacttca ccggtggtcg gcacggcgtc ggactggggc agggcacgcc gaagcagcag
     3901 gccgaggccg cggtgcgcga cctcgagcgc gtgttccccg ggatcgccgc cgcgcgcggc
     3961 aatgcgcgcg aggcacgcat gcactggccg accaatccgt gggtgctggg cagctatgcc
     4021 tgcttccggc cgggcgattg ggcgggcttg ggcggggtga tgggcgagtc cgtgggcggc
     4081 ctgcacttcg ccggcgaaca ttgctcggtc gagtcgcagg gcttca
//
LOCUS       DAOOCX010000188         4120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_20483_length_4120_cov_10.461501, whole genome shotgun
            sequence.
ACCESSION   DAOOCX010000188 DAOOCX010000000
VERSION     DAOOCX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20483_length_4120_cov_10.461501"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PK399_12045"
     CDS             complement(<1..755)
                     /locus_tag="PK399_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13892.1"
                     /translation="MSLTPAEPAIFRGVIALELRDGTPPQHAALAAEAAGDLVAMLGR
                     DLATLVPGVRDCDLALLAAHFDPAEALRPGWPLHRRASELLQRAPGQAQGARMVGFGA
                     DGAGAVPMPLQCDPDLHGGGLRILPFVLHGDAARQAGDALEDLLLDRGMAAADTALAL
                     QDGLGAQVEHARYLSLHDLAAMIALQYRNSGLDALWPLLEAALLEPEAEAWLDAPPEP
                     LARYGDGEVRIALLDPTAWRARNAAGESDCARL"
     gene            834..2111
                     /locus_tag="PK399_12050"
     CDS             834..2111
                     /locus_tag="PK399_12050"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPW13893.1"
                     /translation="MQHGRPVAILGGVRIPFCRQNTAYADVGNLGMSVRTLGALVEKY
                     GLHGQQLGEVAMGAVIKHSSDWNLGREAALSSGLSPLTPGITLQRACGTSLDTIIHIA
                     GKIATGQIEAGIGGGSDTTSDVPIVYGKALRHRLLQAAAAKTTKAKLAAFKGFRFSEL
                     KPDFPGVAEPRTGKSMGQHCEDMAKEWNISRDSQDELAVASHHKLAAAYARGFFDDLV
                     VSFRGVSRDNILRPDSSIEKLATLKPAFDKTSGRGTLTAGNSTPLTDGASACLLATDE
                     WAEQHGHEVLCHLRDAQVSAVDFVHGEGLLMAPTVAVAEMLNRNNLTLQDFDFYEIHE
                     AFAAQVLCTLRAWEDEAYCRNRLGLDAPLGRIDPAKINPNGSSLAAGHPFAATGARIV
                     ATAAKELKQRGGGRCLISICTAGGMGVVAILER"
     gene            2194..3525
                     /locus_tag="PK399_12055"
     CDS             2194..3525
                     /locus_tag="PK399_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPW13894.1"
                     /translation="MKIPVSPPPFGQLFDANAGEFSRLLRLRIGPEVRGKYEHWDHLR
                     HLAPPEGLSSEQWWLAIKLARTTAARDLPLKDRDGRPFRVVLTDSIAEKLHTITRECS
                     GSLRGLDSIAADRAKERFLIRSQIEEAMTSSQLEGAGTTTAVAKEMLRSGRAPRDHGE
                     RMIHNNYLAMQELKRWRDQPLTPDAVFEIHRLLTADTLDDPAQAGRFRTADDNIVVED
                     GEGRILHMPPHADELPMRLQALCDFANGEHEAGFLHPVIRAIALHFQIGYDHPFCDGN
                     GRTARTLFYWSMLRSGYWLTEFLSVSSVFKRAKAKYLRAYLYTETDEADLGYFVDHHL
                     DVILGAIDGLHGWLTRKAEERQRAEGMLRPGSRLGGKLNHRQRELLLDAVRHPDKSYR
                     IDRHMQIQDVSYQTARSDLLALVKLKLMRNERIGNAFVFTPAKDLVEKLKA"
     gene            complement(3550..>4120)
                     /locus_tag="PK399_12060"
     CDS             complement(3550..>4120)
                     /locus_tag="PK399_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13895.1"
                     /translation="RVVTNGMSQYSRNERNANAGIVVGITPEDYAPHGEGPLAGIALQ
                     RHWESQAFVLGGGDYSAPAQRVGDFIRNRPSTAFGAVLPSYTPGVTLGSLDEALPRYA
                     IDAIREALPAFDKQIRGFAMDDALLTGVETRTSSPLRITRGEDGHSLNTRGLFPAGEG
                     AGYAGGILSAGVDGIKTAEAVARSIVAGT"
BASE COUNT          741 a         1344 c         1345 g          690 t
ORIGIN      
        1 tccagccgcg cgcaatcgga ttcgcccgcg gcattgcgtg cccgccacgc cgtgggatca
       61 agcaaggcga tccgcacttc gccatcgccg taccgcgcga gcggttccgg cggcgcgtcc
      121 agccaggctt cggcctcggg ttccagcagg gcggcttcca gcagcggcca caatgcgtcc
      181 aagccgctgt tgcgatattg cagcgcgatc atcgcggcga ggtcgtgcag gctgagatag
      241 cgcgcgtgtt cgacctgcgc cccaaggccg tcctgcagcg ccagcgcggt gtccgccgcg
      301 gccatgccgc gatcgagcag caggtcttcc agcgcatcgc cggcttgccg cgcggcatcg
      361 ccatgcagca cgaacggcag gatgcgcagc ccgccgccgt gcaggtcggg gtcgcactgc
      421 agcggcatcg gcacagcgcc cgcgccatcc gcgccgaagc ccaccatgcg tgcgccttgc
      481 gcctgtccgg gtgcgcgctg caacaactcc gaggcacgcc tgtgcagcgg ccaaccgggg
      541 cgcaaggctt cggccggatc gaaatgcgcg gcgagcaggg caaggtcgca gtcgcgcacg
      601 ccgggcacca acgtggccag gtcgcgaccg agcatcgcca ccaggtcgcc agcggcctcg
      661 gcggcaagtg ccgcatgctg cggcggggtg ccatcgcgca gctccagggc gatcacgccg
      721 cggaagatcg cgggctcggc gggcgtcaag gacatgtgga gcttcggctc gggaaggcgg
      781 cgttagactg cggccattat gcccgccgct gcgggcgcag ccaggtgaag cccatgcaac
      841 acggccgtcc cgtcgccatc ctcggtggtg tccgcatccc gttctgccgg cagaacacgg
      901 cctatgccga tgtcggcaac ctcggcatgt cggtgcgcac gctgggcgcg ctggtcgaga
      961 agtacggcct gcacggccag cagcttggcg aagtcgcgat gggcgcggtg atcaagcatt
     1021 cgtcggactg gaacctcggc cgcgaggctg cgttgagttc cggcctgtcg ccgctgacgc
     1081 ccggcatcac gttgcagcgc gcctgcggca cctcgctgga cacgatcatc cacatcgccg
     1141 gcaagatcgc gaccgggcag atcgaagccg gcatcggcgg cggttcggac acgacgtccg
     1201 acgtgccgat cgtctacggc aaggcgctgc gccatcgcct gctgcaggcc gccgcggcca
     1261 agaccaccaa ggccaagctg gcggccttca agggcttccg gttctccgaa ctcaagccgg
     1321 acttccccgg cgtggccgag cctcgcaccg gcaagtcgat gggccagcac tgcgaggaca
     1381 tggccaagga atggaacatc tcgcgcgact cgcaggacga actggcggtc gcctcgcacc
     1441 acaagctggc cgcagcgtat gcgcgcggct tcttcgacga cctggtggtg agcttccgcg
     1501 gcgtctcgcg cgacaacatc ctgcgcccgg acagcagcat cgagaagctg gccacgctga
     1561 agcctgcgtt cgacaagacc tcgggcaggg gtacgctcac ggcaggcaat tccaccccgt
     1621 tgaccgacgg cgcctccgcc tgcctgctgg ccaccgacga gtgggccgag cagcatggcc
     1681 atgaagtgct ctgccacctg cgcgacgcgc aagtatcggc ggtcgatttc gtccacggcg
     1741 aaggcctgct gatggcgccg accgtggccg tcgccgagat gctcaaccgc aacaacctga
     1801 ccctgcagga tttcgatttc tacgagatcc acgaagcctt cgccgcgcag gtgctgtgca
     1861 ccctgcgtgc gtgggaggac gaggcctact gcaggaatcg cctcggcctg gatgcgccgc
     1921 tgggccgcat cgatcccgcg aagatcaacc cgaatggttc ctcgctcgcc gccggccatc
     1981 cgttcgccgc caccggtgcg cgcatcgtcg ctacggcggc gaaggaactg aagcagcgcg
     2041 gtggtggccg ttgcctgatt tcgatctgca ccgccggcgg catgggcgtg gtggcgatcc
     2101 tggaacgata aaattcttat taatcagtag cttagtaaga tgatgtcaat ctattactaa
     2161 ttcagaacaa attagtagta gattgacaga cgtatgaaaa tccccgtctc accgcccccc
     2221 tttggccaac tgtttgacgc caatgcgggt gagttcagtc gactgctgcg cctccgcatc
     2281 ggaccggagg ttcgcggcaa gtacgaacac tgggatcact tgcgccactt ggcgccaccc
     2341 gagggtttga gcagcgagca atggtggctg gcgatcaagt tggcgaggac gaccgcagcg
     2401 cgcgatctac cgttgaagga ccgggatggc cggccgttcc gggtcgtact gaccgattcg
     2461 atcgcagaga aactgcacac gatcacccgc gaatgttcgg ggtccctgcg tgggctggac
     2521 agcatcgccg ccgaccgcgc gaaggaacgt ttcctgatcc gctcgcagat cgaggaagcg
     2581 atgacttcct cgcaattgga gggcgcggga accactaccg cagttgccaa ggagatgctc
     2641 cgcagtggca gggcacccag ggatcatggc gagcgcatga tccacaacaa ctatctggcg
     2701 atgcaggagc tcaagcggtg gcgcgatcaa ccgctgacgc cggatgcggt gtttgaaata
     2761 caccgactgc tcaccgccga cacgttggat gatcctgcgc aggcgggcag gttccgcacg
     2821 gctgacgaca atatcgtggt ggaggacgga gagggacgca tcctgcacat gccaccgcat
     2881 gcggacgaac ttccgatgcg gttgcaggcg ctttgtgatt tcgccaatgg cgagcacgag
     2941 gcaggcttct tgcacccggt gatccgtgcc atcgccctgc atttccagat cggttacgac
     3001 catcccttct gtgacggcaa cggacgaacc gcgcgcaccc tgttctattg gtcgatgttg
     3061 cggtccggtt attggctgac cgaatttctt tcggtctcca gtgtattcaa gcgtgcgaaa
     3121 gccaagtacc tgcgtgcata cctgtacacg gaaacggacg aggcggattt gggctatttc
     3181 gtcgatcacc acctcgatgt gatcctcggt gccatcgacg gactgcatgg ctggcttaca
     3241 cgcaaagccg aggagcgcca acgcgcggaa ggcatgctgc gtcctggctc gcgcctcggc
     3301 ggcaaactca accaccggca gagggaacta ttgttggatg cggttcgaca cccggacaag
     3361 agctaccgca tcgaccgcca tatgcagatc caggacgtca gctatcagac agcccgctcg
     3421 gatcttctgg cgctggtgaa actcaagttg atgcgcaatg agcgtatcgg caacgcattc
     3481 gtgttcacgc cagcgaagga tctagtcgaa aaattgaagg catagcgcag cgaatgccgc
     3541 ggatcggctt caggttcccg caacgatgct ccgcgccacc gcttccgcgg tcttgatgcc
     3601 gtccacgccg gccgagagga tgccgccggc ataccccgcg ccttcgccgg cggggaacaa
     3661 cccacgggtg ttcaggctgt ggccgtcttc gccgcgggtg atgcgcaagg gcgatgacgt
     3721 gcgcgtttcc acgccggtga gcagggcatc atccatcgcg aagccgcgga tctgcttgtc
     3781 gaaggcgggc agcgcttcgc ggatggcgtc gatcgcatag cgcggcagcg cttcgtccag
     3841 cgagccgagc gtcacccccg gcgtgtacga cggcagcacc gcgccgaatg cggtggatgg
     3901 acgattgcgg atgaagtcgc cgacccgttg tgccggtgcg gagtaatcgc cgccgcccag
     3961 cacgaaggcc tgcgattccc agtgccgttg caacgcgata ccggccaacg ggccttcgcc
     4021 atggggcgcg tagtcctccg gtgtgatgcc gaccacgatg ccggcattgg cgttgcgctc
     4081 gttgcgcgag tactggctca tgccgttggt gaccacgcgg
//
LOCUS       DAOOCX010000189         4098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_20630_length_4098_cov_8.090774, whole genome shotgun sequence.
ACCESSION   DAOOCX010000189 DAOOCX010000000
VERSION     DAOOCX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20630_length_4098_cov_8.090774"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /gene="prpB"
                     /locus_tag="PK399_12065"
     CDS             complement(<1..652)
                     /gene="prpB"
                     /locus_tag="PK399_12065"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HPW13896.1"
                     /translation="MSETAGARFRAALTAESPLQVMGAITAYAGLMAKRTGYKALYLS
                     GGGVAANSLGVPDLGISTMEDVLTDARRIVDATGLPLLVDIDTGWGGAFNIGRTIRSF
                     ERIGVAAVHMEDQVGQKRCGHRPGKEVVSTQEMVDRVKSAVDARTDAGFVIMARTDAA
                     AVEGLDSAIERAVAYVEAGADMVFPEAMTSLEDYRKVKAAVKVPILANLTEFGSTPF"
     gene            complement(716..1723)
                     /locus_tag="PK399_12070"
     CDS             complement(716..1723)
                     /locus_tag="PK399_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HPW13897.1"
                     /translation="MSAPIAEFANDARDFALADMVAAGEPLVIRGLCRDWPLVQAAKR
                     SFGEFAQAVAALDNGSRVDVLRMPPDAEGVVGYNAALDGFNYRHFKVGVTDALQRLAA
                     YSRMQGPVPGLALQSALVSECLPGFLATHPMPCLPASVQPRLWAGNRVVTPAHFDASH
                     NIAVVACGRRRFTLFPTDQVANLYIGPLDFAPTGAAITLARPDKPDFDHYPRLREALA
                     NALIAELEPGDALYMPPLWWHQVESLEPLNALVNYWWMPAQLEGRPTAPALGALLHAR
                     LAFAALPPAERQAWRTLFDHYVFGEGDPAAHIPEARRGALGELGPEALAALKERIRSG
                     L"
     gene            complement(1833..2816)
                     /locus_tag="PK399_12075"
     CDS             complement(1833..2816)
                     /locus_tag="PK399_12075"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPW13898.1"
                     /translation="MKAPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQMLELP
                     MEKAQAALKGVMMELEDCAFPLLAGMVGTDDAEVAFKDADYALLVGARPRGPGMERKD
                     LLLENAKIFTAQGAALNKVASRNVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRL
                     DHNRALSQLANKAGVAVADIEKLVVWGNHSPTMYPDYRFATVGGESLKDRIGDADWNA
                     NTFIPQVGKRGAAIIEARGLSSAASAANAAIDHIRDWALGTDGKWVTMGVPSDGSYGI
                     PEGVMYGVPVTCANGEYTRIEGLPVDDFSRAAMDKTLAELEEERAGVAHLL"
     gene            complement(2891..4090)
                     /locus_tag="PK399_12080"
     CDS             complement(2891..4090)
                     /locus_tag="PK399_12080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPW13899.1"
                     /translation="MPQLARRMSRAKPSAIMAVAEKAKRLKSEGRDIISFSIGVPNFL
                     PGEHVYAAAREALAKDSGQYGSNRGSDALLDAFLAHIEKIGLTGYARVNCATGIGAKH
                     VIYNLAEALLDEGDTIAFPVPYWTSYMDIAEIVNAKVELLPCPASQDYKMTPAQLDAA
                     LAKKPKVFLFNNPSNPTGMVYTRDEIVALADVLVKYPDTWIISDDIYNRMLFDGLEYV
                     NFVQVHPELRDRVIFIDSLSKTYGMPGWRVGFMAGPESVAKAVTTMNSNHITNIPEIV
                     TAAAIAALTGPQDVPTQKNAEFQAKRDQVMAVMDAIPGVVCPRPQGAFYVFPDISCAF
                     GKSHNGTKIADDVDFCNALLEAKGVACVPGSAFGEPRALRISYTCPTPQLAPGLARIQ
                     EFFAELA"
BASE COUNT          632 a         1367 c         1397 g          702 t
ORIGIN      
        1 agaacggcgt ggaaccgaat tcggtgaggt tggccaggat cggcaccttc accgccgcct
       61 tgaccttgcg gtaatcctcg agcgaggtca tcgcttccgg gaacaccatg tcggcgccgg
      121 cttcgacgta ggcgacggcg cgctcgatcg cgctgtccag accctcgacc gcggcggcgt
      181 cggtgcgcgc catgatcacg aacccggcat cggtgcgcgc atcgaccgcg gacttcaccc
      241 gatcgaccat ctcctgcgta ctcaccactt ccttgcccgg acgatgcccg caacgcttct
      301 gcccgacctg gtcttccatg tgcaccgcgg ccacgccgat gcgctcgaaa ctgcggatgg
      361 tgcggccgat gttgaacgcc ccgccccagc cggtatcgat gtcgaccagc aacggcaggc
      421 cggtggcgtc cacgatgcgg cgtgcgtcgg tcagcacgtc ctccatcgtg gagatgccaa
      481 ggtctggcac gcccagcgag ttggcggcaa caccgccacc ggagaggtac agcgccttgt
      541 aaccggtgcg cttggccatc aggccggcat aggcggtgat cgcgcccatc acctgcagcg
      601 gggattcggc ggtgagggcg gcacggaagc gtgcgcctgc ggtctcggac atgacggcgg
      661 tccctggggc ggagtccgcc attttagcgc gtcgccctga ctcgccggca cccgactaaa
      721 gtcccgaccg gatccgttcc ttcaatgccg ccaatgcctc gggaccgagt tcccccagcg
      781 cgccacgcct ggcctcgggg atgtgcgcgg caggatcgcc ctcgccgaac acgtaatgat
      841 cgaacagcgt gcgccatgcc tgtcgttcgg ccggcggcaa tgcagcgaac gccagccgcg
      901 catgcagcaa cgcgccgagc gccggtgccg ttgggcggcc ctcaagctgg gccggcatcc
      961 accagtagtt caccagcgcg ttgagcggtt ccagcgattc gacctggtgc caccacagtg
     1021 gcggcatgta cagggcatcg ccgggctcca gctcggcgat gagcgcattg gccagcgcct
     1081 cgcgcagtcg cgggtaatga tcgaagtccg gcttgtccgg gcgcgccagg gtgatcgccg
     1141 cgccggtcgg cgcgaaatcc aatgggccga tgtagagatt ggccacctgg tcggtcggga
     1201 acagggtgaa gcggcgccgg ccgcacgcaa ccacggcgat gttgtgcgag gcatcgaaat
     1261 gcgccggggt gacgacccgg ttgcctgccc acagccgcgg ctgcaccgac gccggcaggc
     1321 agggcatcgg atgcgtggcg aggaagcccg gcaggcattc ggacacaagc gcgctctgca
     1381 aggcgagtcc cggcaccggc ccctgcatgc ggctgtaggc cgcgagccgc tgcagggcat
     1441 cggtcacgcc gaccttgaag tgccggtagt tgaagccgtc cagcgccgcg ttgtagccga
     1501 ccacgccttc cgcgtccggc ggcatgcgca gcacgtccac ccggctgccg ttgtcgagcg
     1561 cggccaccgc ctgcgcgaac tcgccgaagg accgcttcgc cgcttgcacc aagggccagt
     1621 cgcggcacag gccacggatc accagcggct cgcccgcagc caccatatcg gccaacgcga
     1681 aatcgcgtgc gtcgttggcg aactcggcga tgggcgcgct catgcaatgg ccgtgcgatc
     1741 agggggagct gcacgcagtg tgcgcgagcg aacgcgcaaa cgaaaacgcc gggctttgcc
     1801 cggcgttccg tggtgcaact ggggcgcctg gatcagagca ggtgcgccac gccggcgcgc
     1861 tcttcttcca gctcggccag ggtcttgtcc atcgccgcgc ggctgaagtc gtcgaccggc
     1921 aggccttcga tgcgggtgta ttcgccattg gcgcaggtca ccggcacgcc gtacatcacg
     1981 ccttccggga tcccgtagct gccgtcggac ggcacgccca tggtcaccca cttgccatcg
     2041 gtgcccagcg cccagtcgcg gatgtggtcg atcgcggcat tggccgccga ggcggcggac
     2101 gacaggccgc gcgcctcgat gatcgccgcg ccgcgcttgc cgacctgcgg gatgaaggtg
     2161 ttggcgttcc agtcggcgtc gccgatcctg tccttcagcg actcgccgcc gaccgtcgcg
     2221 aagcggtagt ccgggtacat ggtcgggctg tggttgcccc acaccaccag cttctcgatg
     2281 tcggccactg ccacgccggc cttgttggcc aactgcgaca gcgcgcggtt gtggtccagg
     2341 cgcagcatcg cggtgaaatt cttcgccggc aggtccggcg cggacttcat cgcgatgtag
     2401 gcattggtgt tcgccgggtt gccgaccacc agtaccttga cgttgcggct ggcgaccttg
     2461 ttcagcgcgg cgccctgtgc ggtgaagatc ttggcgttct ccagcagcag gtccttgcgc
     2521 tccatgcccg ggccacgcgg acgcgcgccg accagcaggg cgtagtcggc gtccttgaac
     2581 gcgacctcgg cgtcgtcggt gccgaccatt ccggccagca gcgggaacgc gcagtcctcg
     2641 agctccatca tcacgccctt cagcgcggct tgggccttct ccatcggcag ttccagcatc
     2701 tgcaggatca ccggctggtc cttgccgagc atttcgccgg aggcgatgcg gaacagcagg
     2761 gcgtagccga tctggccggc ggcgccggtg actgcaacac ggacgggggc tttcatgggg
     2821 tgcgactcct ggatgacagt gcggcgtggg gccgcgtcgg atgttcgatg tggatcaggc
     2881 cgccttcgaa tcaggcaagt tctgcgaaga attcctggat gcgcgccagg cccggtgcca
     2941 gctgcggcgt cgggcaggta tagctgatgc gcagcgcgcg cggctcgccg aacgcggaac
     3001 ccggcacgca ggccacgccc ttggcttcca gcaacgcatt gcagaagtcg acgtcgtccg
     3061 cgatcttcgt gccattgtgc gacttgccga acgcgcagct gatatccggg aatacgtaga
     3121 acgcgccttg gggacgcggg cagaccacgc ccgggatcgc gtccatcact gccatcacct
     3181 ggtcgcgctt ggcctggaac tcggcgttct tctgggtcgg cacgtcctgc ggcccagtga
     3241 gcgcggcgat cgccgcggcg gtgacgatct cggggatgtt ggtgatgtgg ttggaattca
     3301 tcgtggtgac cgccttggcc accgactccg gtccggccat gaagcccacg cgccaccccg
     3361 gcatgccata ggtcttggac agcgaatcga tgaagatcac gcggtcacgc agctccgggt
     3421 ggacctgcac gaagttcacg tattccagtc cgtcgaacag catccggttg tagatgtcgt
     3481 cgctgatgat ccaggtgtcc gggtacttca ccagcacgtc ggccagcgcg acgatctcgt
     3541 cgcgggtgta gaccatgccg gtcggattgg acggattgtt gaacaggaac accttgggct
     3601 tcttcgccag cgcggcgtcg agctgggccg gcgtcatctt gtagtcctgc gacgccgggc
     3661 acggcagcag ttccaccttg gcgttgacga tctccgcgat gtccatgtag ctggtccagt
     3721 acggcaccgg gaaggcgatg gtgtcgcctt cgtccagcag cgcctcggcc aggttgtaga
     3781 tcacgtgctt ggcgccgatc ccggtggcgc agttgacccg cgcgtagccg gtcaggccga
     3841 tcttctcgat gtgggccagg aaggcgtcca gcaacgcgtc gctgccgcgg ttgctgccgt
     3901 actggccgct gtccttggcc agtgcctcgc gcgcggcggc atagacatgt tcgccgggca
     3961 ggaagttggg gacgccgatc gagaagctga tgatgtcgcg gccttccgac ttcaggcgct
     4021 tggccttctc ggcgacggcc atgatggcgc tgggcttggc gcgactcatg cgccgggcga
     4081 gttgaggcat cgtggggg
//
LOCUS       DAOOCX010000190         4032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_21132_length_4032_cov_6.784259, whole genome shotgun sequence.
ACCESSION   DAOOCX010000190 DAOOCX010000000
VERSION     DAOOCX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21132_length_4032_cov_6.784259"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /gene="cas2"
                     /locus_tag="PK399_12085"
     CDS             complement(<1..339)
                     /gene="cas2"
                     /locus_tag="PK399_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPW13900.1"
                     /translation="MNAPRAWLISYDITDPRRLIRLHRFLVRQATPVQYSVFHFEGSP
                     AQMGRLMVEIETRIDPDTDDVRGYQLPARLQVDTLGRGSLPSGTALLSADAEALHALL
                     RPPPSMGGARS"
     gene            complement(336..1136)
                     /locus_tag="PK399_12090"
     CDS             complement(336..1136)
                     /locus_tag="PK399_12090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HPW13901.1"
                     /translation="MVTPARRTHAAAALPPRKPLYLLASTPARMDAGPDHLVLRRDQG
                     APLRFPLARLCRIVCNRHVAWSGQALALCLEHGVPITWVDGRGHALGCTQGHQDEALP
                     FATLLETYLELPDWPARFANWRARRRLETLTTTAQRAARAGREFDPIAFSELKRELVY
                     NGTHPLVFPEQGQAWCEAIAMDRLQRQGLQACYWGFGASRLALGVELGALLWAELNLE
                     CGALPAGVAPGQSVARLFEAWAHEHEGRLLLHLGDLERHLAREVEAWQ"
     gene            complement(1153..2136)
                     /gene="cas1"
                     /locus_tag="PK399_12095"
     CDS             complement(1153..2136)
                     /gene="cas1"
                     /locus_tag="PK399_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HPW13902.1"
                     /translation="MATLLLDRADLEVRFADGALALYENGTRSQTVPLRLLDRVVIQG
                     ARTRLDSTVLLKLAEAGAAILLQSPRMSRRVAIVLGPAHNDAAIRLAQTRQVLDAEFC
                     DAWSRKLVRSKLRHQLRLLDTAMAQRPDRRKPLFDARGGVADALATVVAEPAGSARLR
                     GLEGAAARAYFHGLTALFAPELAFTGRNRRPPRDPVNACLSLGYTLLHFEAVRSAHAA
                     GLDPLLGFYHRPAFGRESLACDLIEPLRPTVDAWVWRQFSTGQLRAEHFTDDRGACLL
                     GKTGRGHFYAGWERAARFPRRWLRLACARLARGMAREGKQWLEEAEDPDLF"
     gene            complement(2143..2442)
                     /locus_tag="PK399_12100"
     CDS             complement(2143..2442)
                     /locus_tag="PK399_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPW13903.1"
                     /translation="MPAAIMPKRDFYLAAYDVADPRRLVAALALVRGYVTGGQKSVHE
                     VFLTAGARHELLHDMALLLDETRDRFFLLRLDPSARTYALGVATKPADPDYFYVG"
     gene            2572..2967
                     /locus_tag="PK399_12105"
     CDS             2572..2967
                     /locus_tag="PK399_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13904.1"
                     /translation="MPRHPQKPHRPSSADQRSGERCGERMARARRTRDELANNAWLRF
                     LCEVSTAWDSAKNEAALERIRAAVQPGARKRRLGRLGRAMLQALLALSWEMLLAVSFP
                     VVALLIQVVVQLGVYAFMAYVIYKLLTVW"
     repeat_region   3165..3979
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3236..3272
                     /rpt_unit_seq="gtctcaatccctttgaattcagggcatcagttcgaac"
BASE COUNT          689 a         1297 c         1398 g          648 t
ORIGIN      
        1 cgatcgcgcc ccgcccatgc tcgggggcgg tcgcagcagc gcgtgcaggg cttcggcgtc
       61 ggcagacagc agcgcggtgc cgcttggcag gctgccgcgg cccagggtgt cgacctgcag
      121 gcgcgcgggg agctggtagc cgcgcacgtc gtcggtatcg gggtcgatac gcgtctcgat
      181 ctcgaccatc agccgtccca tctgcgccgg gctgccctcg aagtggaaca ccgagtactg
      241 caccggtgtg gcctgacgga ccaggaagcg atgcagccgg atcagtctac gtgggtcggt
      301 gatgtcgtag ctgatcagcc aggcgcgcgg cgcgttcatt gccatgcttc gacctcgcgg
      361 gcgaggtggc gctcgaggtc gccgaggtgg agcagcaggc ggccttcgtg ctcgtgcgcc
      421 caggcttcga acaggcgggc aacgctctga ccaggggcga cgccggcggg cagcgcgccg
      481 cattcgaggt tgagctccgc ccacaacagt gcgccgagct cgaccccgag cgccagccga
      541 ctcgccccga agccccagta gcacgcctgc aggccttggc gctgcaggcg gtccatggcg
      601 atcgcctcgc accaggcctg cccctgctcg gggaagacga gcggatgggt gccgttgtag
      661 acgagctcgc gcttgagctc ggagaaggca atggggtcga actcccgccc ggcgcgtgcg
      721 gcgcgctgag cggtggtggt cagcgtctcg agccgacggc gcgcgcgcca gttggcgaag
      781 cgcgcgggcc agtccggcaa ctccagatag gtctccagga gcgtggcgaa gggcaaggct
      841 tcgtcctgat ggccttgcgt gcaaccgagg gcgtggccgc ggccgtccac ccaggtgatc
      901 ggtacgccgt gctccaggca gagcgcgagt gcctggccgc tccaggcgac atggcggttg
      961 cacacgatgc ggcacaggcg ggcaagcgga aaacgcagcg gcgcgccctg gtcgcggcgc
     1021 agcaccagat ggtccggccc ggcatccatg cgtgccggcg tgctggcgag caggtagagc
     1081 ggcttgcgcg gcggcagcgc ggcggcggca tgcgtacgac gggcgggcgt aaccatggtc
     1141 gtgtctccgc gttcagaaca ggtccgggtc ctcggcctct tccagccatt gcttgccctc
     1201 gcgcgccatg ccgcgggcga gcctggcgca ggccagtcgc aaccagcgcc gcgggaagcg
     1261 ggcggcgcgt tcccagccgg cgtagaagtg cccgcggccg gtcttgccga gcagacaagc
     1321 gccacggtcg tcggtgaaat gctcggcgcg cagctggccg gtggaaaatt gccgccacac
     1381 ccaggcatcg accgtcggac gcagcggctc gatgagatcg caggcgagcg actcgcgacc
     1441 gaaggccggg cggtgataga agccgagcag cgggtcgagc cccgcggcgt gggcgctgcg
     1501 caccgcctcg aaatgcaaca gggtatagcc gagcgacaga caggcattca ccgggtcgcg
     1561 cggtgggcgc cggttgcggc cggtaaaggc gagctcgggg gcgaacagcg ccgtcaggcc
     1621 gtggaagtac gcacgcgccg ctgcgccctc gagcccgcgc aggcgagcac taccggcagg
     1681 ctcggcgacg acggtggcga gcgcgtcggc aacgccgccg cgtgcgtcga acagcggctt
     1741 gcggcggtcg ggccgctgcg ccatcgcggt gtcgagcagg cgcagctggt ggcgcaactt
     1801 gctgcgcacc agcttgcgcg accacgcgtc gcagaactcc gcatccagca cttgccgggt
     1861 ttgtgcaagc cggatcgcgg cgtcgttgtg cgccgggccg agcacgatcg ccacccgccg
     1921 gctcatgcgc gggctctgca gcagaatggc ggcgccggcc tcggcgagct tgagcagcac
     1981 cgtgctgtcg agccgggtgc gtgcgccctg gatcaccacc cggtcgagca agcgcagcgg
     2041 cacggtctgg ctgcgggtgc cgttctcgta cagtgcgagc gcgccatcgg cgaaacggac
     2101 ttcgaggtcg gcacgatcga gcagcaaggt ggccatggca gttcatccca cgtagaagta
     2161 gtcggggtcg gccggcttgg tcgccacgcc gagcgcatag gtgcgcgcgc tcgggtcgag
     2221 gcgcagcagg aagaagcggt cgcgcgtctc gtcgagcagc agtgccatgt cgtgcagcag
     2281 ctcatggcgc gcgcccgcgg tgaggaagac ctcatgcacc gacttctgcc cgccggtcac
     2341 gtagccgcgc accaaggcca gcgcggcgac cagccggcgc ggatcggcga cgtcgtaggc
     2401 ggcaaggtag aaatcgcgct tgggcatgat ggcggcgggc acggtggttt gcgggtgagc
     2461 ccatcatggc gccctgccgg gccgtcgtca gcgccggcaa gcttgcgatc gcgcggcaag
     2521 gaccgggata cgccgacact ggcggcctgt tcaagcagcc ggccaaaccc catgccccgt
     2581 cacccgcaaa agccgcaccg accgtcgtct gccgaccagc gttccggcga gcgctgcggc
     2641 gagcggatgg cgcgtgcccg ccgtacacgc gacgagttgg ccaacaatgc gtggctccgc
     2701 tttctgtgcg aggtgagtac cgcctgggac agcgccaaga acgaggcggc tctcgagcgc
     2761 atccgcgcag cagtgcaacc aggcgcccgc aagcgtcgcc tcgggcgcct ggggcgggcc
     2821 atgcttcagg cgctgttggc gctgtcctgg gaaatgctgc ttgcggtgtc gttcccggtg
     2881 gtggccttgc tgatccaggt cgtcgtgcag ctgggcgtct acgccttcat ggcgtacgtc
     2941 atctacaagt tgctgacggt ctggtagcag gcgtgacagc caagccagcg cagcgcggca
     3001 gcagaccgtg gccattgcgc agggcgatcc atccggcctc gcagcgtccc gcaggaggcc
     3061 tcccgagcgc tgctccagaa ggtcgcgccg tgcccgaggc ggccgagagg ggtactgcgt
     3121 tcgttcggac gaacgccttc cgcgacccac tgctgcgctg acttctatcg atccctttga
     3181 attcagggca tcagttcgaa cgactcaatg ctcgccgcag caccgaaacc ggagggtctc
     3241 aatccctttg aattcagggc atcagttcga acccccgtgg atgatgcgcg ctctcgaaga
     3301 cgcgcgtctc aatccctttg aattcagggc atcagttcga actgcgggaa tacgatcagg
     3361 ttatcggccg gttctgtctc aatccctttg aattcagggc atcagttcga acaggtcgaa
     3421 acatcgttcc atgacggctt tgaagcgtct caatcccttt gaattcaggg catcagttcg
     3481 aacacgtgga ggtgttcaag cacgggccgg aggacaagtc tcaatccctt tgaattcagg
     3541 gcatcagttc gaacccgtcg tctacggggt ctacgaatat gacacgcgtc tcaatccctt
     3601 tgaattcagg gcatcagttc gaacccttca ggctgaactc gatgcactga aggcgtgaaa
     3661 gtctcaatcc ctttgaattc agggcatcag ttcgaactcg ccacgaaatc cgacaaggag
     3721 aaggccgcgt tgtctcaatc cctttgaatt cagggcatca gttcgaacgc tcgacgcgga
     3781 cacgattgag gcagcaaaga agtctcaatc cctttgaatt cagggcatca gttcgaacgt
     3841 cggttctcgg tcgaaacggc accgaatgaa gtctcaatcc ctttgaattc agggcatcag
     3901 ttcgaaccac ccgaggagtg cgggtgcctc gatgcgccca cggtctcaat ccctttgaat
     3961 tcagggcatc agttcgaacc tccggccggt ggaacgccga gaggggtgag ggcggtctca
     4021 atccctttga at
//
LOCUS       DAOOCX010000191         3992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_21423_length_3992_cov_8.839726, whole genome shotgun sequence.
ACCESSION   DAOOCX010000191 DAOOCX010000000
VERSION     DAOOCX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21423_length_3992_cov_8.839726"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PK399_12110"
     CDS             <1..309
                     /locus_tag="PK399_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182421.1"
                     /note="catalyzes the formation of
                     2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinol
                     from 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HPW13905.1"
                     /translation="AHVVHPLAGQGVNLGLRDVAALREHVRAAKARGGDIASPSRLAR
                     WARERRSENAVNAHAFEAINRVYSNDAMLPTLLRGHALGIAGKLPPLARAFWRHAAGV
                     "
     gene            complement(313..1359)
                     /gene="rlmM"
                     /locus_tag="PK399_12115"
     CDS             complement(313..1359)
                     /gene="rlmM"
                     /locus_tag="PK399_12115"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HPW13906.1"
                     /translation="MNALLCYCRAGFEPELAAELTERAAQAGFAGYARTERNSGFVLF
                     ACEDADDLSRALPWSTLVFARQKLRLFADLQALDPRDRITPIQAALPADIRFGELVME
                     HPDSDAGKPMSGLARSLGNALRPALRKAGTLTKEDNPRLLRLHACMLDGDHALLASGD
                     PEDSAPWPLGIPRLRMHADAPSRSALKLEEALLVLLDDEERKRLLRDGMRAADLGAAP
                     GGWTWVLTRQGLRVQAIDNGPLRQHVLDNGLVEHLRADGFSWQPQQPLDWMVCDMVEQ
                     PRRVAERMATWFREGWCRHAIFNLKLPMKKRWQETQLCLELFASQAEKPLTIRAKQLY
                     HDREEITVFATPAR"
     gene            complement(1486..2061)
                     /locus_tag="PK399_12120"
     CDS             complement(1486..2061)
                     /locus_tag="PK399_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPW13907.1"
                     /translation="MLYAQVHLTLPAWVHDAVDVGRVYATDAEKLRLAIELSQRNVEA
                     ATGGPFGAAVFGPDDRIIAIGVNRVVPHACSVAHAEMMAYMLAQGRTQRLRLNRDADD
                     NPIGPITLATSSQPCCQCYGATVWAGIDRLLIGARAEDVMALTEFDEGPLPADWIGEL
                     NARGIEVVRDIERDAACAVLKAYGELGGAKY"
     gene            complement(2061..3803)
                     /locus_tag="PK399_12125"
     CDS             complement(2061..3803)
                     /locus_tag="PK399_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPW13908.1"
                     /translation="MTPPNTAAEAAPAKTDFIRQIVREDLANGKHAAIKTRFPPEPNG
                     YLHIGHAKAICLDFGVAAEFGGSCNLRLDDTNPAREDPEYVRAIQDDVRWLGFDWASL
                     RHASDYFGVYYLAALKLIRQGDAFVCDLSADEVREYRGSLTEPGRNSPYRERSVEENL
                     DLFTRMRAGEYADGTRTLRAKIDMASGNINLRDPALYRIKHVEHQNTGAEWPIYPMYD
                     FAHALGDAVEGITHSLCTLEFEDHRPLYDWCVDKVDLAGNPDLLEPLVPQGLPKEAGK
                     PRQIEFSRLNFNYLVMSKRKLLAMVEDRLVDGWDDPRMPTLQGIRRRGYTPSALRLMV
                     DRVGISKQNSLLDISILEGALRDDLDAHAPRRMAVIDPLKVVLTNLADDHQESLTFSN
                     HPKDATQGERQIPFSRELWIEREDFEEIPPPGFKRLTVGGDVRLRGAGILRCDEAIKD
                     AAGRVVELHCTLDPDSRPGMEGANRKVKGTIHWVSAMHAIPAEIRLYDRLFTVPNPDA
                     EDDGKTYRDYLNPASRKVVTGYVEPAAADCAPEQGFQFERLGYFVADRYDHVAGTPVF
                     NRSVTLRDTWAGKA"
BASE COUNT          597 a         1287 c         1426 g          682 t
ORIGIN      
        1 gcgcacgtgg tgcatccgct cgccgggcag ggcgtgaacc tgggcctgcg cgatgtcgcc
       61 gctttgcgcg aacatgtgcg tgcggcgaag gcgcgtggtg gcgatatcgc atcgccgtcg
      121 cggctggcac gctgggcgcg cgaacgacgc agcgagaacg ccgtgaatgc gcatgcgttc
      181 gaggcgatca accgcgtgta cagcaatgac gcaatgctgc cgacgttgtt gcgcggacat
      241 gcgctgggaa tcgcaggaaa gctgccgccg ctggcgcgcg cgttctggcg gcacgcggcc
      301 ggggtatgag catcagcgag caggcgtcgc gaacaccgtg atctcttcgc ggtcgtggta
      361 cagctgcttg gcgcggatcg tcagcggctt ttccgcttgt gacgcgaaga gctcgaggca
      421 cagctgggtt tcctgccagc gcttcttcat cggcagcttg aggttgaaga tcgcgtggcg
      481 gcaccagccc tcgcggaacc aggtggccat gcgctcggcg acgcgacgcg gttgttcgac
      541 catgtcgcag accatccagt ccagcggctg ctgcggctgc cagctgaagc cgtcggcgcg
      601 caggtgttcg accaggccgt tgtccagcac gtgttgccgc aacgggccgt tgtcgatcgc
      661 ctgcacgcgc aggccctggc gggtcagcac ccacgtccat ccgccgggag cggcacccag
      721 gtcggcggcg cgcatgccat cgcgcagcag gcgcttgcgt tcctcgtcat ccagcaggac
      781 cagcaaggcc tcttccagct tcagcgcgga gcgcgagggc gcatcggcgt gcatgcgcaa
      841 acgcgggatg ccgagcggcc acggcgcgct gtcttcgggg tcgccgctgg ccagcagcgc
      901 gtggtcgcca tccagcatgc aggcatgcag gcgcaacagt cgcgggttgt cttccttcgt
      961 cagcgtgccc gccttgcgca acgccgggcg cagggcatta cccaggctgc gcgccaggcc
     1021 cgacatcggc ttgccggcat cggagtccgg atgctccatc accagctcgc cgaagcggat
     1081 gtccgcaggc agcgcggcct ggatcggggt gatgcgatcg cgcggatcca gtgcttgcag
     1141 atcggcgaac aggcgcagct tctgccgcgc gaacaccagc gtcgaccacg gcaacgcgcg
     1201 cgacaagtcg tccgcgtctt cacaggcgaa gagtacgaag ccgctgttgc gctcggtgcg
     1261 cgcgtagccg gcgaatccgg cctgcgcggc gcgctcggtc agttccgcgg cgagttccgg
     1321 ttcgaagccg gcgcggcagt agcagagcag ggcgttgatc tgctccctcc cttgcgaagg
     1381 caggggaggg ttggggtggg gttgctctgt catgtgggag gtttccttgt ttggagcaga
     1441 ccccctcccg gcctccccct gcattcgcaa ggggagggga agagctcagt acttcgcgcc
     1501 gcccagttcg ccgtacgcct tgagcaccgc acaggcggca tcgcgttcga tgtcgcgcac
     1561 gacctcgatg ccgcgcgcat tcagttcgcc gatccagtcc gccggcagcg ggccttcgtc
     1621 gaattcggtc aacgccatca cgtcctcggc gcgcgcaccg atcagcaggc ggtcgatgcc
     1681 ggcccacaca gtcgcgccgt agcactggca gcagggctgc gaggacgttg cgagggtgat
     1741 cgggccgatc gggttgtcgt cggcatcgcg gttcaggcgc aggcgctggg tgcggccctg
     1801 cgccagcatg taggccatca tctcggcatg cgccacgctg catgcgtgcg gcaccacgcg
     1861 gttcacgccg atggcgatga tgcggtcgtc cgggccgaac accgcggcgc cgaacggacc
     1921 gccggtcgcc gcttccacgt tgcgctgcga cagttcgatg gccaggcgga gtttctccgc
     1981 atcggttgca tagacgcggc cgacatcgac ggcgtcgtgc acccatgcgg gcaaggtcag
     2041 gtggacttgc gcgtacagca tcaggccttg cccgcccagg tatcgcgcag ggtcacgctg
     2101 cggttgaaca ccggcgtgcc ggcgacgtgg tcgtagcggt cggcgacgaa ataaccgagg
     2161 cgttcgaact ggaacccctg ctcgggcgcg cagtccgccg cagcgggctc cacgtaaccg
     2221 gtgacgacct tgcgcgaggc ggggttgagg tagtcgcgat aggtcttgcc gtcgtcctcg
     2281 gcatccgggt tcggcacggt gaacaggcgg tcgtacaggc ggatctcggc gggaatcgca
     2341 tgcatcgcgc tgacccagtg gatggtgccc ttgaccttgc ggttggcgcc ttccatgccg
     2401 ggccgcgagt ccggatccag cgtgcaatgc agttcgacca cgcggccggc ggcgtccttg
     2461 atggcttcat cgcaacgcag gatgccggcg ccgcgcaggc gcacgtcgcc gcccacggtc
     2521 aggcgcttga agcccggcgg cggaatttcc tcgaagtcct cgcgctcgat ccacagctcg
     2581 cgcgagaacg ggatctggcg ctcgccctgg gtcgcgtcct tcgggtggtt ggaaaacgtg
     2641 agggattcct ggtggtcgtc cgccaggttg gtgagcacca ccttgagagg gtcgatgacg
     2701 gccatgcggc gcggcgcatg cgcgtcgagg tcatcgcgca acgcgccttc caggatcgag
     2761 atgtcgagca gcgagttctg cttgctgatg ccgacgcggt cgaccatcag gcgcagcgcg
     2821 gacggcgtgt agccgcgacg gcggatcccc tgcagggtcg gcatgcgcgg gtcgtcccag
     2881 ccgtcgacca gccggtcttc gaccatcgcc agcagcttgc gcttgctcat gacgaggtag
     2941 ttgaaattga gccgcgagaa ttcgatctgg cgaggcttgc cggcttcctt tggcaggccc
     3001 tgcggcacca gcggctccag caggtcggga ttgccggcca ggtccacctt gtccacgcac
     3061 cagtcgtaca gcgggcggtg gtcctcgaac tccagcgtgc acagcgaatg ggtgatgccc
     3121 tcgaccgcgt cgcccagggc gtgggcgaag tcgtacatcg ggtagatcgg ccattcggcg
     3181 ccggtgttct ggtgctcgac gtgcttgatc cggtacagcg ccgggtcgcg caggttgatg
     3241 ttgccgctgg ccatgtcgat cttggcgcgc agggtgcgcg tgccatcggc gtactcgccg
     3301 gcgcgcatgc gggtgaacag gtcgaggttc tcctcgacgc tgcgctcgcg gtacggcgaa
     3361 ttgcggccgg gctccgtcag gctgccgcgg tattcgcgga cctcatcggc gctcaggtcg
     3421 cagacgaagg catcgccctg gcggatcagc ttcaatgcgg ccaggtagta caccccgaag
     3481 tagtccgagg catggcgcag gctggcccag tcgaagccga gccagcgcac gtcgtcctgg
     3541 atggcgcgca cgtactcggg gtcttcgcgg gccgggttgg tgtcgtccag gcgcaggttg
     3601 cagctcccgc cgaactccgc ggccacgccg aagtccaggc agatcgcctt ggcatggccg
     3661 atgtgcaggt agccgttggg ctccggcggg aagcgggtct tgatcgccgc gtgcttgccg
     3721 ttggccaggt cctcgcggac gatctggcgg atgaagtcgg tcttggccgg ggcggcttcg
     3781 gcggcggtat tcggaggggt cagggacacg tggaatcgca cttgcggaac agcgcgctag
     3841 tgtagcggag gcaagccaga tcggagtttg cggccacttg gcacgctacg tgcatgcacc
     3901 tccctccggg gggaatccat gcgcaccgtc tacgaggccg cgaacctgat cgatgcccac
     3961 ctggtgcgcc aggcactgga ggccgaaggc at
//
LOCUS       DAOOCX010000192         3931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_21879_length_3931_cov_6.840815, whole genome shotgun sequence.
ACCESSION   DAOOCX010000192 DAOOCX010000000
VERSION     DAOOCX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21879_length_3931_cov_6.840815"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PK399_12130"
     CDS             complement(<1..279)
                     /locus_tag="PK399_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13909.1"
                     /translation="MNAPDLQAVIARMGASARAASAHMAAAPTAAKNRALIALARLLR
                     ADVDALVSANARDLDAATTAGTAGPLLDRLKLTPKIIETVAEGCEQIAA"
     gene            complement(639..2087)
                     /locus_tag="PK399_12135"
     CDS             complement(639..2087)
                     /locus_tag="PK399_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016849214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPW13910.1"
                     /translation="MTLPTAFVHPLRSGTLALLVAGIAGCGSMIPEFVRPAAPVPAAF
                     PGTGTQAVPDSTPAADLEWQRYFSDPQLQRLIDLALKNNRDLRVAVLNIEQARAQYQV
                     RRADELPTVNGGITGSRSPRVGSGSGSSINSTYTAGLSVTAYEFDFFGRVRSLSQAQL
                     AQFAGTEEARKTVQISLVASVANAWLGLLADDELLELTRRTLASREESMKLTRLKFDH
                     GASSELDLRQAESLLEGARVALAQQTRQRAQDENALALLLGQPVPPDAASATTPVGAS
                     ALAQTVLPDVPAGLPSELLSRRPDIRQAEQQLIAANANIGAARAAFFPRITLSTSVGI
                     ASTQLSDLFKGGSLAWSFAPQLVLPIFDAGRNQANLDAAQVNRDIATAQYEKAVQSAF
                     REVADALAGRATLGEQLRAQQAQAAAEQVRTKLADLRYRNGASSYLDVLDAQRSLFTA
                     QQAVVQAQAAERQNRVTLYKVLGGGWKDAATQ"
     gene            complement(2084..>3931)
                     /locus_tag="PK399_12140"
     CDS             complement(2084..>3931)
                     /locus_tag="PK399_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW13911.1"
                     /translation="KAMGQISGAIVGVTVVLISVFVPLAFFSGAVGNIYRQFSAVMVS
                     AIGFSAFLALSLTPALCATLLKPVEAGHHHAKTGFFGWFNRGFSRTAKGYEGVVARIL
                     TRAARYLIIYAAIIGAVVVMFGRLPTSFIPNEDQGNILVNVQLPPGATQGRTTEVMKQ
                     AEAWMLKQPDVQSMVSVLGFSFSGTGQNAALGFVTLKDWDEREGAEHTAQALVARAFG
                     ALGQIRDAFIFPVSPPAIRELGRANGFAMRLQDRGGNGHDALLAARNQLLGMAAKSTL
                     LVSVRPDGLEDAPQLQLDVDRDKAGALGVTFGAINTTLSTALGSSYVNDFPNAGRMQR
                     VVVQADAPARMQPDDLLQLNALNSAGQPVPLASFVTTRWINGPMQTVRYNGYPAMRIQ
                     GEPAKGVSSGAAIAEMERLAGQLPPGFAYEWTGQSREEKLSGSTALFLFAFSLLAVFL
                     CLAALYESWSIPLAVILVVPLGVLGVVLGIGWRGMENDVFFKVGLITIIGLSAKNAVL
                     IIEFAKELHAQGKGVVEAVLEAAHLRFRPILMTSLAFILGVLPLVLASGAGSASQRAI
                     GTGVMGGMLTATLLAVFFVPVFYVVVMRVFSRRPAIADAQPIAGPGDAP"
BASE COUNT          621 a         1350 c         1341 g          619 t
ORIGIN      
        1 cgccgcgatc tgctcgcagc cttcggccac cgtctcgatg atcttcggcg tcagcttcag
       61 gcggtcgagc agcggcccgg cagttcccgc ggtcgtcgcg gcgtccaggt cgcgcgcgtt
      121 ggcgctgacc agcgcgtcga catcggcgcg cagaagccgc gcgagcgcga tcagcgcgcg
      181 gttcttggcc gccgtcggcg cggcggccat atgcgcactg gcggcgcggg cggacgcgcc
      241 catgcgggcg atcacggctt gcaggtcggg tgcgttcatg agaccgattg tcccaaattc
      301 gctgtacctt gaccttcgcg cggtgctgcc gatggcgcgg ggcgaccggc tccgctcatg
      361 tcctccgggc cggaaaagac cggccctccc cctctacttc gctgcgccag tcaccccacg
      421 ccctcgacag caggcaacgt cgtcgcatgc gaatgcattc aaaccccccg attgcttcga
      481 tggtggcagg agctctgtga cgggtcgctg tgtggagtga agtaaagggg gagaggcggg
      541 cacagcacgc ctcggaggac atgaacgcaa cacagcggca cgctgcagtg ctcacgctcc
      601 gaactcgggt gagcgatccg ttcaagtgcg acttgttgtc actgcgtcgc agcgtccttc
      661 cacccaccgc ccaacacctt gtacagcgtg acgcggttct gccgttcggc cgcctgcgcc
      721 tgcaccaccg cctgctgcgc ggtgaacagc gagcgctgtg catcgagcac gtcgaggtag
      781 ctcgacgcgc cattgcggta gcgcaggtcg gccagcttcg tgcgcacctg ttcggcagcg
      841 gcctgcgcct gctgcgctcg cagttgttcg cccagtgtcg cgcgacccgc cagcgcatcg
      901 gcgacctcgc gaaaggcgct ctgcacggcc ttctcgtact gggcggtggc gatgtcgcga
      961 ttgacctgcg ccgcgtcgag gttggcctga ttgcgtcctg cgtcgaagat cggcagcacc
     1021 agctgtggcg caaagctcca ggccaggctg ccgcccttga acaggtccga cagctgggtg
     1081 ctcgcgatgc cgacgctggt gctcagcgtg atccgaggga agaacgccgc acgtgccgcg
     1141 ccgatgttgg cgttggccgc gatcagttgc tgctcggcct gccggatgtc gggccggcgg
     1201 ctcaacagct cggacggcag acccgccggc acgtcgggca gcacggtctg cgccagtgcc
     1261 gatgcgccca cgggtgtggt cgccgacgcc gcatccggcg gcaccggctg ccccagcagc
     1321 agggccagcg cgttctcgtc ctgggcccgc tgccgggtct gctgcgcgag cgcgacccgc
     1381 gcaccttcga gcagcgattc ggcttgtcgc aagtcgagtt cggacgacgc gccgtggtcg
     1441 aacttgagcc gagtcagctt catcgattcc tcgcgcgacg ccagcgtgcg acgcgtcagt
     1501 tcgagcaact cgtcatcggc cagcagcccg agccaggcgt tcgccaccga tgcaaccagg
     1561 ctgatctgca cggtcttgcg cgcttcctcg gtaccggcga attgcgccag ctgcgcctgg
     1621 ctgaggctgc gcacccggcc gaagaaatcg aactcgtacg cggtgaccga caggccggcc
     1681 gtgtaggtgc tgttgatgct gctgccggag ccgctgccca cgcggggcga gcgtgatccg
     1741 gtgatgccgc cattgacggt gggcagctcg tcggcgcgcc gcacctggta ttgcgcgcgc
     1801 gcctgctcga tgttcagcac cgcgacgcgc aggtcgcggt tgttcttcag cgcgaggtcg
     1861 atcagccgct gcagttgcgg atcgctgaag tagcgctgcc attccaggtc agctgcgggc
     1921 gtgctgtcgg gaaccgcctg cgtgcccgtg ccggggaagg ccgcaggcac cggcgccgcg
     1981 gggcgcacga actcgggaat catcgaaccg cagccggcga tgccagccac cagcagcgca
     2041 agcgtgccgg accgcagagg gtgaacgaag gcagttggca gcgtcatggt gcatctcctg
     2101 ggcccgcgat cggctgcgcg tcggcgatcg ccggacgccg gctgaacacg cgcatcacca
     2161 cgacatagaa caccggcacg aagaacaccg ccagcagcgt cgccgtgagc atgccgccca
     2221 tcacgcccgt gccgatcgcg cgctggctgg ccgaaccggc cccgctggcg aggaccagtg
     2281 gcagcacgcc gaggatgaag gcgagcgaag tcatcaggat cggccggaaa cgcaggtgcg
     2341 cggcctcgag cacggcctcg accacgccct tgccctgcgc atgcaactcc ttcgcgaact
     2401 cgatgatcag caccgcgttc ttcgccgaca gcccgatgat ggtgatcagg ccgaccttga
     2461 agaacacgtc gttctccatc ccgcgccagc cgatgccgag caccacgccg agcacgccga
     2521 gcggcacgac caggatcacc gccagcggaa tcgaccagct ctcgtacagc gcggccaggc
     2581 aaaggaagac ggccaacagg gagaaggcga acaggaacag cgccgttgat cccgacagct
     2641 tttcctcgcg cgactggccg gtccactcgt aggcgaatcc tggcggcagt tgtcccgcga
     2701 ggcgctccat ttcggcgatg gccgcgccgc tgctgacgcc cttggccggc tcgccctgga
     2761 tgcgcatcgc cgggtagccg ttgtagcgca cggtctgcat cggcccgttg atccaccggg
     2821 tggtcacgaa ggacgccagc ggcaccggct ggccggcgct gttgagcgcg ttgagctgca
     2881 gcaggtcatc gggctgcatg cgcgcaggcg catcggcctg caccaccacg cgctgcatgc
     2941 gcccggcgtt cgggaagtcg ttgacgtagg acgagcccag cgcggtcgac agcgtcgtgt
     3001 tgatggcgcc gaacgtcacc cccagcgcgc ccgccttgtc gcggtcgaca tcgagctgga
     3061 gctgtggggc gtcttccagg ccgtcggggc gcaccgacac cagcagcgtg ctcttggcgg
     3121 ccatgccgag caactggttg cgcgcggcca gcagcgcgtc gtgtccgttg ccgccccggt
     3181 cctgcagccg catcgcgaaa ccattggcgc ggcccagttc gcggatggcc ggcgggctga
     3241 ccggaaagat gaatgcgtcg cggatctggc cgagcgcgcc gaaggccctg gcgaccagcg
     3301 cctgcgcggt gtgttcggcg ccctcgcgtt cgtcccagtc cttcagggtg acgaagccca
     3361 gcgcagcgtt ctggccggtg cccgagaaac tgaaaccgag cacgctgacc atgctctgga
     3421 cgtcgggctg cttcagcatc caggcctcgg cctgcttcat cacctcggtg gtgcggcctt
     3481 gcgtggctcc cggcggcaac tgcacgttga cgaggatgtt tccctggtcc tcgttcggga
     3541 tgaacgaggt cggcaggcga ccgaacatca ccaccaccgc gccgatgatc gcggcgtaaa
     3601 tgatcaggta gcgtgctgcg cgcgtcagga ttcgggcgac aacgccctcg tagcccttgg
     3661 cggtgcgcga gaagccgcgg ttgaaccagc cgaagaagcc ggtcttcgcg tggtggtggc
     3721 ccgcctctac cggcttgagc agcgtcgcgc agagtgcggg tgtcagcgag agggccagga
     3781 acgccgagaa gccgatcgcg ctgaccatca cggccgagaa ctggcggtag atgttgccca
     3841 cggcgccgct gaagaatgcc agcggaacga acaccgatat cagcaccacc gtcacgccga
     3901 cgatggcgcc ggagatctgg cccatcgcct t
//
LOCUS       DAOOCX010000193         3907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_22073_length_3907_cov_8.000519, whole genome shotgun sequence.
ACCESSION   DAOOCX010000193 DAOOCX010000000
VERSION     DAOOCX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22073_length_3907_cov_8.000519"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..591
                     /locus_tag="PK399_12145"
     CDS             46..591
                     /locus_tag="PK399_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HPW13912.1"
                     /translation="MSEFFIGQIMMAGFSFAPKYFGQCNGQLLPIAQNQALFSLLGTQ
                     YGGNGTTTFALPDLRSRTPIGYASSVDPGWQPPAVQIGQTGGVENVTLLQTNIPAHTH
                     AMNASTSPGDNRIPATRVFATSTNSAAAANLYAPSSGTAVVMNAQSVAPAGGNQPHPN
                     LQPYTTINFCIALQGIFPSRN"
     gene            695..1225
                     /locus_tag="PK399_12150"
     CDS             695..1225
                     /locus_tag="PK399_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HPW13913.1"
                     /translation="MSQPFVGEIRMFGFGRTPNGWQACDGSLLSIAEYEVLFTLIGTT
                     YGGDGQVTFAVPDLRGRLPIHQGTGPGLSNYVIGQVSGTETVTLNTQQMPAHTHGMIA
                     TTAASTALTPGTNLLPGTVSGDVFYATDLTGATPAPMATQSTTVSGGSQPHDNTMPTL
                     TVQYCIAWAGIYPSQS"
     gene            1317..1856
                     /locus_tag="PK399_12155"
     CDS             1317..1856
                     /locus_tag="PK399_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HPW13914.1"
                     /translation="MTEPFIGQIQQFGFNFNPRGWAFCNGATLPIQQNTALFALLGTQ
                     YGGNGQTTFQLPNFAGRGGCQQGQGPGLTPRSLGETFGTNTVTLTSTQIPQHNHGITA
                     FAQSDGNKKSGSPSNNSALSSLGSGTAKPFITAPLNTQFAPNMIQPSAGGGQPHQNQQ
                     PYLGVNFCIALQGIFPSFP"
     gene            1915..2418
                     /locus_tag="PK399_12160"
     CDS             1915..2418
                     /locus_tag="PK399_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW13915.1"
                     /translation="MTTPALLIERGFSLRAAHDGDLPWLRDLYASTRAEEMAPVPWPE
                     IAKRSFLDQQFGLQHQHYLNHYGDTDFLAIEHAERGPVGRYYLQRTAPDHLLVDISLF
                     PALRGSGVGSVLIRQSQQDAAALGRGMRLHAQESNPGARRLYERLGFVAEEGAEGTHR
                     PMRWSPI"
     gene            complement(2511..2801)
                     /locus_tag="PK399_12165"
     CDS             complement(2511..2801)
                     /locus_tag="PK399_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13916.1"
                     /translation="MELLTLEHFAGCVNETFLAAINDMDLEFVLVEARPLQGHAPNAV
                     RQPFSLLFRNTAAFLFPQQIYPMRHPRIGETGIFLVPVAQEQAGFLYQAVFN"
     gene            3045..>3907
                     /locus_tag="PK399_12170"
     CDS             3045..>3907
                     /locus_tag="PK399_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPW13917.1"
                     /translation="MKENPIRSLPGIPFLLGVLIVAAFSVWLFATGIGSDPDNNGAAS
                     PARIFAAILLAFTAFLSLIGLYTVQPNQAAVVSLFGKYVGTVKENGLRWNNPFFSKKK
                     ISQRVRNFESSKLKVNELDGSPIEIAAVIVWQVVDASEAVYNVDDYESFVHIQSESAL
                     RTMATSYPYDQHEDGQVALRSHAAEVSQHLKDELGERLADAGVAVIDARITHLAYAQE
                     IAQAMLQRQQANAIIAARTRIVAGAVGMVQMALAELEKNGVVNLDEERKAQMVSNLLV
                     VLCGERGTQPIV"
BASE COUNT          740 a         1385 c         1151 g          631 t
ORIGIN      
        1 cgacgcaaga tgcgttgcgc ccaccatcca agggaggata tttcgatgag cgaatttttc
       61 atcggccaga tcatgatggc cggtttcagc tttgcgccca agtacttcgg gcaatgcaac
      121 gggcagttgc tgcctatcgc acagaatcag gccttgttct cgctgctggg gacgcagtac
      181 gggggcaacg gcaccaccac tttcgctttg ccggacctgc gcagccgaac gccgatcggc
      241 tacgcaagct cggtcgaccc cggctggcag ccgccggcgg tgcagatcgg ccagaccggt
      301 ggcgtcgaga acgtgacgct gctgcagacc aacataccgg cgcatacgca cgccatgaat
      361 gcatcgacta gtccgggcga caaccggatt cccgcgacac gcgtgttcgc caccagcacc
      421 aacagcgcag cagctgccaa cctgtatgcg ccctcgagcg gcaccgcagt ggtgatgaac
      481 gcccaatcgg tggcacccgc cggcggcaat cagccgcatc cgaacctgca gccctacacc
      541 acgatcaatt tctgcatcgc gctgcagggc atcttcccgt cgcgcaactg acgtctggtc
      601 gagtcggcgc gcgcgacgcg cccgctacga acatcgcggt tctggcgcgt ccttcgacgc
      661 gcggccgccc accgaatcaa cggagtgcat tcccatgtct caaccttttg tcggagagat
      721 ccgcatgttc ggcttcggcc ggacacccaa cggctggcaa gcctgcgatg gcagcctgct
      781 gtcgatcgcc gaatacgaag tcctgttcac cctgatcggc accacttacg gcggcgacgg
      841 ccaagtcact ttcgccgtac ccgatcttcg cggccgcctg cccattcacc agggcacagg
      901 ccccggcctg agcaactacg tcatcggcca ggtctcgggc acggaaacgg tcaccctgaa
      961 tacccagcag atgccggcgc atacccacgg catgatcgcc acgaccgccg cgtcgaccgc
     1021 tctgacgccc ggcaccaacc tgctaccagg cacggtcagc ggcgacgttt tctacgcgac
     1081 cgatctcacc ggtgccaccc cggccccgat ggcgacgcag tcgaccaccg tctccggtgg
     1141 ctcgcagccg cacgacaaca cgatgccgac gctgacggtg cagtactgca tcgcctgggc
     1201 gggcatctac ccgtcgcagt catgacccgt tcgcgcgtcc aaaaccattt tccacagcgc
     1261 catgccggcg cggccgcgca ttcatccgcg gcaaacctca tcggaggaac caggtcatga
     1321 ccgagccctt tatcggccaa atccagcaat tcggcttcaa cttcaatccc cgcggctggg
     1381 cgttctgcaa tggcgccacg ctgccgatcc agcagaacac cgcactgttc gcgctgctgg
     1441 gcacgcagta cggcggcaac ggccagacca ccttccagct gccgaacttc gccggccgcg
     1501 gcggctgtca gcaggggcag gggccggggc tcacgccgcg atcgctcggc gaaaccttcg
     1561 gcaccaatac cgtcaccttg accagcaccc agattccgca gcacaaccac ggcatcactg
     1621 cgttcgccca gtccgacggc aacaagaaga gcggttcgcc gagcaacaac agcgcactct
     1681 cctcgttagg cagcggcact gccaagccat tcatcacggc tccactcaac acccagttcg
     1741 cacccaacat gatccaaccc agcgcaggcg gcgggcagcc ccaccagaac cagcaaccgt
     1801 atctcggcgt gaatttctgc atcgcgctgc agggcatctt cccgtcgttc ccctgacgtg
     1861 gcgatggcga caccgcaaac gtcggcgccg ttcccccggg aacgcgccga ccgcatgacc
     1921 actcccgcgc tcctcatcga gcgcgggttt tctttgcgcg ccgcgcacga cggcgacctg
     1981 ccgtggctgc gtgacctgta cgccagcacc cgcgccgagg aaatggcgcc ggtaccgtgg
     2041 ccggaaatcg cgaaacgcag ttttctagat cagcagttcg ggctgcagca tcagcactat
     2101 ctgaatcact acggcgacac cgatttcctg gcgatcgagc acgccgaacg cggcccggtc
     2161 ggtcgctact acctgcagcg caccgcgccc gatcatctgc tggtcgacat cagtctgttt
     2221 ccggcactgc gcggcagcgg cgtgggcagt gttctgatcc gccagagcca acaggacgcc
     2281 gcggcgctcg gccgcggcat gcgcctgcat gcgcaggagt ccaatcccgg cgcgcgtcgg
     2341 ctttacgaac gcctgggctt cgtcgccgag gagggcgccg agggcacgca tcggccgatg
     2401 cgctggtcgc cgatatgacg caggagggcc ttcaggcccg atcttttccg aacgatcacc
     2461 gcgatccttg tagagctcgg gcctgaaggc cctcctgcga caatcgcctc tcagttgaat
     2521 accgcctgat acaggaaccc ggcctgctcc tgcgccaccg gcaccaggaa aatgccggtc
     2581 tcgccgatcc gcgggtggcg catcggatag atctgctgcg ggaacaggaa cgccgcggta
     2641 ttgcggaaca gcagcgagaa cggctgacgc acggcgttcg gtgcatggcc ctgcaacggc
     2701 cgcgcttcga ccagcacgaa ctccagatcc atgtcgttga tcgcggcgag gaacgtctcg
     2761 ttgacgcacc cggcgaagtg ttccagcgtc agcaattcca tttagcgcgg ctccccagcc
     2821 tgcgctgccc atccggcagc aggcgacatc ctcgccgcag ccggtggccc gcacaagccc
     2881 gacgacctca gggacagagg tagtgacgac cgctgaacta cctccgctgc catttcgctg
     2941 gcccgcacat ccggattgcc gttcgtcggc ccgccgttga atgattgata tcaatttgat
     3001 atcaaatgcc aaagcccatt ccgggccggc cgacgagacc cgccatgaaa gagaacccca
     3061 tccgctcact gcccggcatc ccgttcctgc tgggtgtcct catcgttgcc gccttctcgg
     3121 tctggctgtt cgccaccggc atcggctcgg atccggacaa caacggcgcc gcctcgcctg
     3181 ccagaatctt cgccgccatc ctgctggcct tcaccgcgtt cctcagcctg atcggcctgt
     3241 acaccgtgca gcccaaccag gccgcggtgg tcagcctgtt cggcaagtac gtgggcacgg
     3301 tcaaggaaaa cggcctgcgc tggaacaacc cgttcttcag caagaagaag atcagccagc
     3361 gcgtgcgcaa cttcgagagc agcaagctca aggtcaacga actggacggc agcccgatcg
     3421 agatcgccgc ggtgatcgtg tggcaggtgg tcgatgcgtc cgaggccgtc tacaacgtcg
     3481 acgactacga gagcttcgtc cacatccagt ccgaatccgc gctgcgcacg atggccacca
     3541 gctatccgta cgaccagcac gaggacggcc aggtggcgtt gcgcagccat gccgccgagg
     3601 tcagccagca cctgaaggac gagttgggcg agcgcctggc cgatgccggc gtggcggtga
     3661 tcgatgcgcg catcactcac ctcgcctacg cccaggaaat cgcccaggcg atgctgcagc
     3721 gccagcaggc caacgcgatc atcgccgcgc gcacgcgcat cgtcgccggt gcggtgggca
     3781 tggtgcagat ggctctggcc gagctcgaaa agaacggcgt ggtcaacctg gacgaggagc
     3841 gcaaggcgca gatggtcagc aacctgctgg tggtcctgtg cggcgagcgc ggcacccagc
     3901 cgatcgt
//
LOCUS       DAOOCX010000194         3806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_22917_length_3806_cov_7.267662, whole genome shotgun sequence.
ACCESSION   DAOOCX010000194 DAOOCX010000000
VERSION     DAOOCX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22917_length_3806_cov_7.267662"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(254..382)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            515..1564
                     /locus_tag="PK399_12175"
     CDS             515..1564
                     /locus_tag="PK399_12175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPW13918.1"
                     /translation="MQIRRLNTLRAIAALIVLVGHYSNRAQLWDAVLGTRAPQLGVML
                     FFLLSAFLMSVLYIDRVPDAHAMRRYGLARAGRVLPLFVAVVLLSWALGKVGIPWLAA
                     SVYDIPDWKAIASHLAFVYGAQVLWTIPAEIHFYLVFALLWWLRPRLPWAIPLFCTAV
                     LALYASGHWPVGPKQPWMGFPIDLPVLRGLPYFVAGMAIGMLYLRWQAPARWRHHGFV
                     AALGLILLLYPAIFQRLTGWSYGMGGGESPMWFLPPLLLAMSAIFFVLVFLVPDGNPV
                     LEHRVGDYLGNISYSMYLLHFPVLLAMGQFGLAKGVGGLLLFLGITVLASSLSYRFFE
                     RPMRMRIRAKDAPAT"
     gene            1561..2373
                     /locus_tag="PK399_12180"
     CDS             1561..2373
                     /locus_tag="PK399_12180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW13919.1"
                     /translation="MSMRIAIAIGLAGILVVAGLPPAVGADARSDEQSPIAALRIEPA
                     DGEYLAWADNRLAGPIEVILRAGAGGDVPSDPRLPARASVPAGGSALVARLRPPNGRS
                     GNLQLRMQAVPGSSNARPRDVEYLPPLRGQARIDQGFGGSFSHDDVQNRHALDFAADI
                     GTPVFAARAGTVMQVEAGFRAGGIASRDNRGRANFIRILHDDGSMALYAHLAVDGVLV
                     RIGQEVTAGQRIGLSGNTGFSTGPHLHFAVQVNRGMQLMSIPFRMRGVAPAR"
     gene            complement(2440..>3806)
                     /locus_tag="PK399_12185"
     CDS             complement(2440..>3806)
                     /locus_tag="PK399_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW13920.1"
                     /translation="RAQELAAVNGFVEGMLTEADPEHSQGRDLRVRDVLDVAAAELES
                     SSLSPGAIASLHQVLGTTWLGLGDGARSRAAFDAALAQKALPTALRDELLLGRARGAI
                     VAGDYDTGEAGLAELRARRGALPPAFRIALDESGAELLRERGKQKEAVAALRALLPQA
                     RKVLGADAQRTLGLQLQLASALQLDGDYPAALEVARDATERHVRKFGSRHPQTLYAWN
                     QLGVVENKLGHADEAEAAFRRSATGRVAVLGENHPATVMSQLNLGSLLIERGHAPEGV
                     PLVRSASAWLDANRPEGDDKALVARSVLAYGLEDQGDLDGAEALLRSLLAAQESRGGP
                     GAPDTFAPRNNLAMLLMKRGRLDEALAQFDTLSTQVRARLGADHPFAAIFDSNRGECL
                     AQLGRFDDARRVLEDSHARLKARFGAEHERTRTAAERLAGVYQRLGMQEKASAMRAAD
                     VAKS"
BASE COUNT          547 a         1332 c         1294 g          633 t
ORIGIN      
        1 cctgacagga aagagacggt cgaccgcgcc ctggcggcgg gcgtcgagca catccgccga
       61 cactgcagcc gccgtgcgca tcgcttcgcc gcccggggat agggtcgggc tggcaagggc
      121 attgcgttcg gcaaggatca cggcggcact ccgatggaca tttctgcaaa ggcaggaacc
      181 atcggagcgt tgcggggagt accgggaaga agaaccgact gggtcggcgt cgcggactgc
      241 gcggggcagc tcgcaaccat cttccggtgg acacgcaggt ccccgcagga tttggcaccg
      301 cgaggaaccg gccgcaatgc ggtcggtcca tcaggttgcc ccggcttctt cgggcctgtc
      361 cctcagccgg tctcgatgga tgcgatcagt ctggccacgc gccccggaca tgtcaatcgc
      421 tttatccgga tgaagcgatg taaataacca atcgtcatga cccggcgtca tcgggaatac
      481 tggccatgac cggcaggggc cggcgggaca caccatgcag atacggcgcc tcaacaccct
      541 ccgcgcgatc gcagcattga tcgtgctggt cggccactac agcaaccgtg cccagctctg
      601 ggacgcggtg ctgggcacgc gtgcgccgca actgggggtg atgctgttct tcctgctcag
      661 cgcgttcctg atgagcgtgc tctacatcga ccgcgtgccg gacgcgcacg ccatgcgccg
      721 ctacggactg gcgcgcgccg gccgcgtgct gccgctgttc gtggcggtgg tgctgctgtc
      781 gtgggcgctg ggcaaggtcg gcatcccgtg gctcgccgca tcggtgtacg acatccccga
      841 ctggaaggcg atcgcgtcgc acctggcctt cgtctacggc gcgcaggtgt tgtggacgat
      901 ccccgccgaa atccacttct acctggtgtt cgccctgctc tggtggctgc gcccgcggct
      961 gccgtgggcg atcccgctgt tctgcaccgc cgtgctggcg ctctacgcca gcggccactg
     1021 gccggtcggc cccaagcaac cctggatggg attcccgatc gacctgccgg tgctgcgcgg
     1081 cctgccctac ttcgtggccg gcatggcgat cggcatgctc tacctgcgct ggcaggcacc
     1141 ggcgcgctgg cgccaccatg ggttcgtggc ggcgctggga ctgatcctgc tgctgtatcc
     1201 cgccatcttc cagcgattga ccggctggag ctacggcatg ggtggtggcg agagcccgat
     1261 gtggttcctg ccgccgctgc tgctggcgat gtcggcgatc ttcttcgtgc tggtgttcct
     1321 ggtgccggat ggcaacccgg tgctggagca tcgcgtcggc gattacctgg gcaacatctc
     1381 gtactcgatg tacctgctgc acttcccggt gctgctggcg atggggcaat tcggcctcgc
     1441 caagggggtc ggcggcctgc tgttgttcct gggcatcacc gtcctggcat cgtcgctcag
     1501 ctatcgcttc ttcgaacgcc cgatgcggat gcgcatccgc gcgaaggatg cgcccgcaac
     1561 atgagcatgc ggatcgccat cgccatcggc ctcgccggca tcttggtggt tgccggcttg
     1621 cccccggcag tcggcgcgga tgcacgaagc gatgagcagt cgcccatcgc ggccctgcgc
     1681 atcgagcccg ccgatggcga atacctggcg tgggccgaca accgcctggc cggtccgatc
     1741 gaagtgatcc tgcgcgcggg cgcgggcggc gacgtgccca gcgacccacg cttgcccgca
     1801 cgggccagcg tccccgccgg tggcagcgcg ctggtggcgc ggttgcggcc gcccaacggt
     1861 cgaagcggca acctgcaact gcgcatgcaa gcggtgcccg gcagttccaa tgcacgcccg
     1921 cgcgatgtcg agtacctgcc gccactgcgg gggcaggcgc ggatcgacca gggtttcggc
     1981 ggcagcttca gccacgacga cgtgcagaac cgccacgcgc tggatttcgc cgccgacatc
     2041 ggcacgccgg tcttcgccgc gcgcgccggc acggtgatgc aggtcgaggc cggcttccgc
     2101 gccggtggca ttgcctcccg cgacaaccgc ggccgggcga acttcatccg catcctccac
     2161 gacgacggca gcatggcgct gtacgcgcac ctcgccgtgg atggcgtgct ggtccggatc
     2221 ggccaggagg tgacggccgg ccagcgcatc ggcctgtccg gcaacaccgg cttcagcaca
     2281 ggtccgcacc tgcatttcgc ggtgcaggtg aatcgcggga tgcagttgat gtcgatcccg
     2341 ttccggatgc gcggcgtggc accggcgcgc tgaccctgca accctgcgga tcggggttgt
     2401 tccgacccgc ggatgacgat gggctgcgcg gatcgctcct caggacttgg cgacgtccgc
     2461 agcgcgcatg gcgctggcct tctcctgcat gccgaggcgt tggtacacgc cggcgagacg
     2521 ctcggccgcg gtgcgcgtgc gttcgtgctc ggcaccaaaa cgcgccttca ggcgggcatg
     2581 actgtcttca agtacgcgcc tggcatcgtc gaagcgcccc aactgcgcca ggcattcgcc
     2641 gcgattgctg tcgaagatgg cggcgaaggg atggtccgct cccagccgcg cgcgtacctg
     2701 tgtcgacagc gtgtcgaatt gcgccagggc ttcgtcgagt cggccgcgct tcatcagcag
     2761 catggccagg ttgttgcgcg gcgcgaaggt atccggggca ccggggccgc cacgcgactc
     2821 ctgcgcggcc agcaggctgc gcaacagcgc ctcggcaccg tccaggtcgc cctggtcttc
     2881 cagcccgtag gccagcacgc tgcgagccac tagcgccttg tcgtcgccct cggggcgatt
     2941 ggcatccagc caggcactgg cgctgcgcac cagcggcacg ccttcgggag cgtgcccgcg
     3001 ctcgatgagc aaggaaccca ggttcagctg gctcatcacc gtcgccggat ggttttcccc
     3061 gagcacggcc acgcggcccg tggcggaacg cctgaacgcc gcttccgctt cgtcggcatg
     3121 gcccagcttg ttctccacca cgccgagctg gttccacgca tacagcgtct gcggatggcg
     3181 cgacccgaac ttgcgcacat gtcgttccgt ggcatcgcgg gcgacctcga gcgcggcggg
     3241 atagtcgccg tccagttgca acgcggacgc cagctgcaac tgcagcccga gcgtgcgttg
     3301 cgcgtccgcg cccagcacct tgcgcgcttg cggcaggaga gcgcgcagcg ccgccacggc
     3361 ctccttctgc ttgccgcgct cacgcaacag ttctgcgccg gattcgtcca gcgcgatgcg
     3421 aaaggccggc ggcaaggcgc cacgccgagc gcgcagctcg gccaggccgg cttcgccggt
     3481 gtcgtagtcg ccggcgacga tggcgccacg cgcacgtccc agcagcagtt cgtcgcgcag
     3541 cgccgtcggc aatgcctttt gtgccaatgc ggcatcgaag gcggcgcgcg aacgcgcacc
     3601 atcgcccagt cccagccagg tggtacccag cacctggtgc aggctggcga tcgcacccgg
     3661 cgacaacgag gacgattcca gctcggccgc ggcgacatcc aggacgtcgc ggacgcgcag
     3721 gtcgcgcccc tgcgaatgct cgggatcggc ctcggtcagc atgccctcca cgaaaccgtt
     3781 caccgccgcc agttcctgcg cgcgcg
//
LOCUS       DAOOCX010000195         3798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_22974_length_3798_cov_3.829548, whole genome shotgun sequence.
ACCESSION   DAOOCX010000195 DAOOCX010000000
VERSION     DAOOCX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22974_length_3798_cov_3.829548"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..245
                     /locus_tag="PK399_12190"
     CDS             9..245
                     /locus_tag="PK399_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPW13921.1"
                     /translation="MIDAHLVRQALEAEGIPAFIRGEALTGGMGELGVFGLLAVMVPE
                     AAWPQARALVEELALGTADGEIGESDDGAAECAY"
     gene            233..2413
                     /locus_tag="PK399_12195"
     CDS             233..2413
                     /locus_tag="PK399_12195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW13922.1"
                     /translation="MRLLNRLLPMLALIGALSVLGSALAWLIHADAPMPGEVRVLVNA
                     GQDGASDTRLIARPGAEPTLNASLDFDLPASRQGPWVLWLPREPLQSILVTGKDAQGR
                     PWTSAPDGFFTPSPSDGFAPAGYAVVLPHGLVGAQRFHVSIRAGLQAAPTPRLIAIDG
                     ALRLTAREMVFASALYAAWATLLIASLALYWAVRDVLFLAHAAYTLMALVFMAVLQGH
                     LYAIPGMDMVAAMGMRGFWLAVLLFNLCSLWLVLQFVDARGSRARLVRRLPMLLPVVA
                     LPDALAFLPMPSASAALQVAAPLAWMLSAPASILAMLDGARRGIPMALAAATLLIALL
                     LAAVAHIAMNRGLLVDGMLVRHGYQVVLVLFSLVLFVGLSSRIGSVRRQLDDETSARR
                     DSEARLRHEQVRTRLTQSLQQVMREVPEDRIAHAAFRLLGAHAGELLGTEDALVLGDD
                     YLGDGKLFVQATAKTTTTARAVQAARALVRDHARNREPVEVRLGAGRRSDDPGDGNYL
                     IVPLRVPAPGWAALVAPVRRMLAAEDKAALVQMARTTVEQADAAHAAFLLRRTAERDA
                     LTGGFNRRTLDQLAARECKIACNRKPLGVLFIDLDWFKRINDTHGHPCGDECLRKVAA
                     TLRAELRPQDVFGRYGGEEFLVLLPGQDAASARLVGERLREAVEAAVVDWQGEALRFT
                     ISIGMAVLREGDDPGKLLARADKALYRAKREGRNRVCAAPAYVD"
     gene            2480..3130
                     /gene="msrA"
                     /locus_tag="PK399_12200"
     CDS             2480..3130
                     /gene="msrA"
                     /locus_tag="PK399_12200"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014089456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPW13923.1"
                     /translation="MLGIGGWKQRMVDPAHALPGRDTPMAVRNAHHVHGRPIAGEFTG
                     LQTVQFGMGCFWGAERKFWRIPGVFTTAVGYAGGVTPNPTYREVCSGETGHAEVVLVA
                     YDPGEASFDGLLKTFWESHDPTQGMRQGNDIGTQYRSAIYCSTQAQFDAAIASRDAYQ
                     RRLLDAGLGGITSEILYPAPAFFYAEDEHQQYLSKNPMGYCGLGGTGVSCPIGTGA"
     gene            complement(3146..3529)
                     /locus_tag="PK399_12205"
     CDS             complement(3146..3529)
                     /locus_tag="PK399_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13924.1"
                     /translation="MKRIACCLLLGLACADAAAAGARCHVIYGGEEWTVDAAPTAQPY
                     RVEGKKIGRYFEFKLVVADQPTTGQSISIYTYGMSTGEPVLIHQALYRPPFDPRADGT
                     GFSGFQYVYEPSKSSELQYWCQALP"
     gene            complement(3526..>3798)
                     /locus_tag="PK399_12210"
     CDS             complement(3526..>3798)
                     /locus_tag="PK399_12210"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPW13925.1"
                     /translation="GHPHVTQGAETYKGKPIIYSLGNFLFNGFDTEATTTGWVLSLRV
                     GREGVREWRTHVARLDANGVPHPAIDAASPCGVAGDPAVRQCKGTP"
BASE COUNT          550 a         1248 c         1375 g          625 t
ORIGIN      
        1 ccgcgaacct gatcgatgcc cacctggtgc gccaggcact ggaggccgaa ggcatccccg
       61 ccttcatccg cggcgaggca ctgacaggcg gcatgggcga actgggcgtg ttcggcctgc
      121 tcgcggtgat ggtccccgag gctgcatggc ctcaggcgcg ggcgctggtc gaggagctgg
      181 cgctgggtac tgccgatggc gaaatcggcg agtccgacga cggagcggcc gaatgcgcct
      241 actgaacagg ttgctgccga tgctggccct gatcggggcg ctgtcggtgc tggggtcggc
      301 cttggcctgg ctgatccatg cggatgcacc gatgccgggc gaggtgcggg tgctggtcaa
      361 cgccggccag gacggcgcca gcgacacgcg actcatcgcg cgtcccggtg cggaacccac
      421 gctcaacgcc agcctcgact tcgacttgcc ggcgtcgcgg cagggaccct gggtgctgtg
      481 gctgccacgc gagcccctgc aatccatcct ggtgaccggc aaggatgcgc aggggcgccc
      541 ttggacaagt gcgccggatg gattcttcac cccgtcgccg agcgatggtt tcgcgcccgc
      601 cggctacgcg gtggtgctgc cgcatggcct cgtcggcgcg cagcgattcc acgtctcgat
      661 tcgcgctggc ctgcaggccg cacccacgcc gaggctcatc gccatcgatg gcgccctgcg
      721 cctgaccgcg cgcgagatgg tgttcgccag cgcactgtat gccgcgtggg caaccctgct
      781 gatcgcgtcg ctggcgttgt actgggcggt gcgcgacgtg ctgttcctgg cccatgcggc
      841 ctacacgctg atggcgctgg tgttcatggc ggtgttgcag gggcatctgt acgccatccc
      901 cggcatggac atggtcgcgg cgatgggcat gcgcggcttc tggctggcgg tgctgctgtt
      961 caacctgtgc agcctgtggc tggtgctgca gttcgtcgat gcgcgcggct cgcgtgcgcg
     1021 gctggtgcgc cgcttgccga tgctgctgcc ggtggtcgcg ctgccggacg cactggcgtt
     1081 cctgccgatg ccatcggcct ccgcggcgtt gcaggtcgcg gcgccgctgg cgtggatgct
     1141 gtccgcgccc gcgagcatcc tggcgatgct ggatggcgcg cgtcgcggca tcccgatggc
     1201 attggccgcg gcgaccctgc tgatcgcgct gctgctggcc gccgttgcgc acatcgcgat
     1261 gaaccgcggg ttgttggtcg acggcatgct ggtccgccac ggctaccaag tcgtgctggt
     1321 gctgttctcg ctggtgctgt tcgtcggttt gagcagtcgg atcggatcgg tgcgccggca
     1381 actggacgat gagaccagcg cgcgccgcga cagcgaggca cgcctgcgcc acgagcaggt
     1441 ccgcacccgc ctcacccaat ccttgcagca ggtcatgcgc gaggtgccgg aagaccgcat
     1501 cgcccatgcc gccttccgcc tgctcggcgc gcacgccggc gaactgctgg gtaccgagga
     1561 cgccctggtg ctgggcgacg actacctcgg cgacggcaag ctgttcgtcc aggccaccgc
     1621 caagaccacg acgacggcgc gggcggtgca ggccgcgcgc gcactggttc gcgaccatgc
     1681 gcgtaaccgc gagccggtcg aggtgcgcct tggggccggt cgccgtagcg acgatccggg
     1741 cgatggcaac tacctgatcg tgccgttgcg cgtgccggcg cccggatggg cggcgctggt
     1801 cgcgccggtc cgacgcatgc tggcggcgga ggacaaggcc gcgctggtgc agatggcgcg
     1861 gaccacggtg gagcaggcgg atgcggcgca cgccgcgttc ctgctgcggc gtacggcgga
     1921 gcgcgacgca ctgaccggcg ggttcaaccg acgcacgctg gaccagctgg ccgcgcgcga
     1981 gtgcaagatc gcctgcaacc gcaagccgct gggtgtgctg ttcatcgacc tggactggtt
     2041 caagcggatc aacgacaccc acggccatcc ctgcggcgac gaatgcctgc gcaaggtcgc
     2101 cgccaccctg cgcgcggaat tgcgcccgca ggacgtgttc ggccgctatg gcggcgagga
     2161 attcctggtc ctgttgccgg ggcaggatgc ggcctcggcg cggctggtcg gcgaacgcct
     2221 gcgggaggcg gtggaagcgg cggtggtgga ctggcagggc gaggcgctgc gcttcaccat
     2281 cagcatcggc atggcagtgc tgcgcgaggg cgacgatccc ggaaagctgc tggcccgcgc
     2341 ggacaaggcg ctgtaccggg ccaagcgcga aggccgcaac cgcgtctgcg cggcgccggc
     2401 ctacgtggac tgatcgagac gggttgcaaa gcaagccgac cgacgccatc cagcaagtcc
     2461 gttccgaagg agattggcga tgctgggcat aggcggctgg aaacaacgca tggtcgatcc
     2521 ggcacacgca ttgcccgggc gcgatacgcc gatggcagtg cgcaacgcgc accacgtgca
     2581 tggccgtccc atcgcaggcg aattcaccgg cctgcaaacc gtgcaattcg gcatgggctg
     2641 cttctggggc gcagaacgca agttctggag gatcccgggt gtcttcacca cggcggtcgg
     2701 ctatgccggt ggtgtcacgc ccaacccgac ctaccgcgaa gtctgttccg gcgaaaccgg
     2761 ccatgccgaa gtggtgctgg tggcgtacga ccccggcgaa gccagcttcg acgggttgct
     2821 gaagacgttc tgggaaagcc acgatcccac ccagggcatg cgccagggca acgacatcgg
     2881 cacccagtac cgatccgcga tctactgctc gacgcaggca cagttcgatg ccgccatcgc
     2941 cagccgcgat gcctaccagc gccggttgct cgatgccggc ctcggcggga tcaccagcga
     3001 gatcctgtat cccgcgccag cgttcttcta tgccgaggac gaacaccagc aatacctgtc
     3061 gaagaacccg atgggctatt gcgggttggg tggcaccggc gtgagttgcc cgatcggcac
     3121 cggcgcctga ggcgctcgcg cgccgtcagg gcagggcctg gcaccagtac tgcagctcgc
     3181 tgctcttgct gggctcgtag acgtactgga agccgctgaa cccggtgccg tcggcgcgtg
     3241 gatcgaacgg cgggcgatac agcgcctggt ggatcagtac cggctcgccg gtggacatgc
     3301 cgtaggtgta gatgctgatg gactggccgg tcgtgggttg gtcggcgacg accagcttga
     3361 attcgaagta acggccgatc ttcttgcctt cgacgcgata tggctgcgcg gtgggcgcgg
     3421 cgtcgacggt ccattcctcg ccgccgtaga tgacgtggca gcgtgcgccg gcggcggctg
     3481 catcggcgca ggcgaggccc agcagcaggc agcaggcgat ccgcttcatg gcgttccctt
     3541 gcattggcgg acggccgggt cgccggcaac accgcatggg ctggcggcat cgatggcggg
     3601 atgcggcacg ccgttggcgt cgagcctggc cacgtgggtg cgccattcgc gcacgccctc
     3661 gcgtcccacg cgcagggaca gtacccagcc ggtcgtggtc gcttcggtgt cgaagccgtt
     3721 gaacaggaaa ttgcccaggc tgtagatgat cggcttgccc ttgtaggtct ccgcgccctg
     3781 ggtcacgtgc ggatggcc
//
LOCUS       DAOOCX010000196         3763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_23302_length_3763_cov_6.136192, whole genome shotgun sequence.
ACCESSION   DAOOCX010000196 DAOOCX010000000
VERSION     DAOOCX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23302_length_3763_cov_6.136192"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..645
                     /locus_tag="PK399_12215"
     CDS             4..645
                     /locus_tag="PK399_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HPW13926.1"
                     /translation="MRIDFVSDVVCPWCAIGLSALEQALQRTQGEVGAEIHFQPFELN
                     PHLPAEGEGIGEHLQRKYGMDQAQLADNQERIRARGAALGFVFDFNARSRIWNTFDAH
                     RLLHWAGIEGGQRALKHALLQAYFSEGRNVSDRATLVDIAGAAGFDVARAQAILGSGE
                     YADEVREAEQFFTRNGISGVPAVIIERKHLVSGGQPVEVFERALREIAAGIPA"
     gene            871..1350
                     /locus_tag="PK399_12220"
     CDS             871..1350
                     /locus_tag="PK399_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13927.1"
                     /translation="MLALLMAATLQVSAGSTAELPVTFTQDDARWPVIVRAGGVVAAS
                     GDALVVRLRGVEITDQPANPSTIPYTHYRVCLARKATEAPWETAGCSDAVKIRRMPVA
                     DETVSLPSQVVSIPANGAVSLADYWLVLEVVSRPVQGRTYSVPSHSQPSLFVAVDSP"
     gene            complement(1387..1737)
                     /locus_tag="PK399_12225"
     CDS             complement(1387..1737)
                     /locus_tag="PK399_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13928.1"
                     /translation="MLSWIRTWLYALAVLGALIVAAWWFVQQTSSYATLDTLLRKHPV
                     VSGEVGKVSSIRLPFFGYGVDVGDGRLDLNFNVRVAGSKGEATVHADFVDGATTDAYL
                     ITAEGSTIPLVIPR"
     gene            1871..2479
                     /locus_tag="PK399_12230"
     CDS             1871..2479
                     /locus_tag="PK399_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPW13929.1"
                     /translation="MPGATMYHEGMRDLQDARDTRRIADRLEEVVVRSAFTDEDRAFI
                     GRSAMFFIATADAAGHPDCSYKGGLPGFVRVLDERTLAIPDYDGNGMYRSWGNVRVNP
                     NVGLLFLDFEQPRRLRINGSASIALDDPLRETFPGCVFVVRLRAEHIFPNCPRYLHRM
                     QLVEHSAHAPRPDYTPPVPAWKGYEVFREFLPARDRGEDAPG"
     gene            2536..3516
                     /locus_tag="PK399_12235"
     CDS             2536..3516
                     /locus_tag="PK399_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13930.1"
                     /translation="MIVPEYWAEAREQARHKGRVITVRRYGWSDDSTEAAQAHANARA
                     REALDAILSGASLPRREPRTNYGIAGVPIREQIVARHEDVVITRNSYGALCLNTPDVL
                     FADVDLAERPSGCVVSIGGLAISAAVGFAVGLLAWHWVAGVALAMVGVWAVNRFWLRR
                     QQAVFDRDGGAEARALALVESFSAAHPDWHLRIYRTPAGLRVLAMHRTFQPDDADAAR
                     LFSALQADRLYTVMCKVQRCFRARLTPKPWRIGIDRRIRPPVAAWSAEQANLPERLQW
                     IARYEQRSTGHAACRYLCALGDTARIDPKAEHVRALHDAMSRADSDLPLA"
     gene            3602..>3763
                     /locus_tag="PK399_12240"
     CDS             3602..>3763
                     /locus_tag="PK399_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HPW13931.1"
                     /translation="MTLALYGHPFSSYTQKVLIALYENATPFEFRCISPDHPEHAGEW
                     LRRWPLRKFP"
BASE COUNT          581 a         1270 c         1305 g          607 t
ORIGIN      
        1 accctgcgca tcgatttcgt gtccgacgtg gtctgcccgt ggtgcgcgat cggcctgtcc
       61 gcgctggaac aggcgctgca acgcacccag ggtgaggtgg gggccgaaat ccacttccag
      121 ccgttcgagt tgaacccgca cctgcccgcg gaaggcgagg ggatcggcga acacctgcag
      181 cgcaagtacg gcatggacca ggcgcaactg gccgacaacc aggagcgcat ccgcgcgcgt
      241 ggcgcggcac tcggcttcgt gttcgacttc aatgcgcgca gccggatctg gaacaccttc
      301 gacgcgcatc gcctgctgca ctgggccggc atcgagggcg gccagcgcgc actcaagcat
      361 gccttgctgc aggcctattt cagcgagggc cgcaatgtct ccgatcgcgc caccctggtc
      421 gacatcgcgg gcgctgcagg gttcgacgtg gcgcgcgcgc aggcgatcct cggttccggc
      481 gaatacgcgg acgaggtgcg cgaggccgag cagttcttca cccgcaacgg catcagcggc
      541 gtgccggcgg tgatcatcga acgcaagcac ctggtctccg gcgggcagcc ggtcgaggtg
      601 ttcgagcgcg ccttgcgcga gatcgcagca ggcattccgg cctgatcgcg cattccatcc
      661 cgcggagccg aagggacgca ttgcggtatg cgcattggcg catcctgtat gccgtttttg
      721 cacatcgcac tgcagcaaga caccggggga cattcccggt atcaaggacg tgccatcgcg
      781 tcatgactga cggacaggtg gggtcatggt gcaggcacgg atggatcgtc ggtccgcccg
      841 tcatcgacga tcatccctgg ggagggaagc atgctggcat tgctgatggc tgcgacattg
      901 caggtgtccg cgggttcgac cgccgagctt ccggtcacct tcacccagga cgatgccagg
      961 tggccggtga tcgtgcgtgc cggcggggtg gtggcggcct cgggcgatgc gctggtggtg
     1021 cgcttgcgtg gcgtggagat cacggaccag ccggccaacc ccagcacgat tccctacacc
     1081 cactatcgcg tctgcctggc gcgcaaggcg accgaggcgc cgtgggagac cgcgggttgc
     1141 tcggatgccg tgaagatccg caggatgccg gtggcggacg aaaccgtgtc gctgccgtcc
     1201 caggtcgtca gcatcccggc gaacggcgcg gtctcgctgg cggactattg gctggtcctg
     1261 gaggtggtgt cgcgcccggt gcaggggcgc acctacagcg tgccgtcgca ttcgcagccg
     1321 tcgctgttcg tggcggtcga cagtccctag gcgggcgggg tgcgccggca cggcgccacc
     1381 gcgcggtcag cgggggatca ccagcgggat cgtgctgccc tcggccgtga tgaggtaggc
     1441 atcggtggtc gccccgtcca cgaagtcggc atggaccgtc gcctcgccct tgctgccggc
     1501 cacccggaca ttgaagttca ggtccagccg gccatcgccc acgtccacgc catagccgaa
     1561 gaatggcagc cggatcgagg acaccttgcc cacctcgccg gacaccaccg gatgcttgcg
     1621 cagcagggtg tccagcgtcg cgtacgaact ggtctgctgc acgaaccacc acgcggccac
     1681 gatgagggcg ccaagtacgg caagggcgta caaccaggtg cggatccagc tcagcatcgg
     1741 gggctgcctg tcgttggagt gcgcctgcga gggtagccga aaccggcgca gtacggtcgc
     1801 gtggatgtcc gtcgccgggc acggccggcg tttcccttga tgagccggtc gccggaacgg
     1861 ggagggggtc atgccgggtg ccacgatgta ccacgaaggc atgcgcgacc tgcaggacgc
     1921 gcgcgacacc cggcgcatcg ccgatcgcct ggaagaggtg gtcgtgcgca gcgccttcac
     1981 cgacgaggat cgcgccttca tcgggcgcag tgcgatgttc ttcatcgcca ccgccgacgc
     2041 cgccggccat cccgattgtt cctacaaggg cggccttccc ggcttcgtgc gggtgctcga
     2101 tgagcgcacc ctggcgatcc ccgactacga cggcaacggc atgtatcgca gctggggcaa
     2161 tgtgcgggtc aacccgaacg tcggcctgct gttcctcgac ttcgagcagc ccaggcgcct
     2221 gcgcatcaac ggcagcgcgt ccatcgcgct ggacgacccg ctgcgcgaga ccttccccgg
     2281 ctgcgtgttc gtggtccgcc tccgagccga acatattttc cccaattgcc cgcgctacct
     2341 gcaccgaatg caattggtcg aacattcggc gcacgcgcca cgccccgact acacgccgcc
     2401 ggtcccggca tggaagggtt acgaagtgtt ccgcgagttc ctgccggcgc gcgatcgcgg
     2461 agaggacgcg ccgggctgag ccaccggcgc gcacttgggc gacaatcccg ccaagcgcga
     2521 tgaccggtac cgatgatgat cgttcccgaa tactgggccg aggcccgcga acaggcccgc
     2581 cacaaggggc gcgtcatcac tgttcgccgg tacggctggt ccgacgacag cacggaggcg
     2641 gcacaggcgc atgcgaatgc gcgcgcccgc gaggcgctgg acgcgattct ttccggtgcc
     2701 agcctgccgc ggcgcgaacc gcgcacgaac tacggcatcg ccggcgtgcc gatccgcgag
     2761 cagatcgtgg cccgccacga ggacgtggtg atcacccgca acagctacgg cgcgctgtgc
     2821 ctcaacaccc cggatgtgct gttcgcggat gtcgacctgg ccgagcggcc atcggggtgc
     2881 gtggtctcga tcggcggctt ggcgatcagc gcggcggtcg ggttcgcggt cggcttgctc
     2941 gcgtggcatt gggtcgccgg cgtggcgctg gcgatggtgg gcgtgtgggc ggtcaaccgg
     3001 ttctggctgc gccggcagca ggccgtgttc gatcgcgatg gtggtgccga ggcgcgagcg
     3061 ctggccttgg tggagtcgtt cagcgccgcg catccggact ggcacctgcg aatctatcgc
     3121 acgccggccg gcttgcgcgt gctggcgatg caccgcacct tccagccgga cgatgccgat
     3181 gccgcgcggc tgttctccgc gctgcaggcg gaccgcctgt acacggtgat gtgcaaggtg
     3241 caacgctgct tccgcgcgcg gctcacgccg aagccatggc ggatcggcat cgaccggcgc
     3301 atccgcccgc cggtcgcggc gtggtcggcg gagcaggcga acctgccgga gcgcttgcaa
     3361 tggatcgcac gctacgagca gcgctcgact ggccacgccg cctgccgcta cctctgtgcg
     3421 ctcggcgaca cggcgcgcat cgaccccaag gccgagcatg tgcgtgccct gcatgatgcg
     3481 atgtcgcgcg cggacagcga cctgccgctg gcgtgacgga cggtctgctt cctgcacgat
     3541 gtcgaaaccg accccgcgcg tgcgtcgtga tggcaaccac ccgatcgacg agaccattgc
     3601 catgaccctt gcgctctacg gccacccgtt ttcctcgtac acgcagaagg tgctgatcgc
     3661 actgtacgag aacgccacgc cgttcgagtt ccgctgcatc tcgcccgacc atccggaaca
     3721 cgcgggcgaa tggttgcggc gctggccgct gcgcaagttc ccg
//
LOCUS       DAOOCX010000197         3709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_23767_length_3709_cov_7.694308, whole genome shotgun sequence.
ACCESSION   DAOOCX010000197 DAOOCX010000000
VERSION     DAOOCX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23767_length_3709_cov_7.694308"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK399_12245"
     CDS             <1..369
                     /locus_tag="PK399_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13932.1"
                     /translation="WEHLGGDGRHSLQAPLATLHAFNGWADKFLVTPAAGLDDRYLGL
                     GGNLGRERAGTRTAWQLAWHDYRADRGGLRYGSEWNASLTVPLAKGLGATVKLADYRA
                     SAFARDTRKLWLQVEYKGRR"
     gene            complement(380..1450)
                     /locus_tag="PK399_12250"
     CDS             complement(380..1450)
                     /locus_tag="PK399_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HPW13933.1"
                     /translation="MNDDWNAGERRWAADAILQLRREAARSADTHLLHMRFAGFPGID
                     FYFKDEAAHPTGSLKHRLARSLFLYALCNGRLREGQAVVDASSGSTAISEAWFARLLG
                     LRFIAVMPACTAPGKIEAVRSLGGHCELTADGECTQSRARAIAGGGACFLDQFGLAER
                     ATDWRGNNNIAESILDQMAREPCPHPAWIVCGVGTGGTSATIGRYLRYRGLDTRLCVA
                     EPVGSGFVRGWRGRDPHATATHPTLIEGIGRPRVEPGFIFDVVDAVVEVEDADSIAAA
                     WRLEELLGRRYGGSSGTNLMACLQLAEGMREQGCNGSIVTLLCDRGDRYAQTLFDHDW
                     LAAHAIDPGPAHARLAARLQAA"
     gene            complement(1447..1848)
                     /locus_tag="PK399_12255"
     CDS             complement(1447..1848)
                     /locus_tag="PK399_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HPW13934.1"
                     /translation="MIAFYPQIKQFHIVIALLSGALFALRGGFALAGARWPRAWPVKW
                     LSYAIDTSLLTAAMMLLTILPWAMFANGWLLAKILLIVAYVVLGVLAMRPHRSGRARA
                     LYYASALLAFAAIYGIARAHHPLGWLARWLA"
     gene            complement(1845..3056)
                     /locus_tag="PK399_12260"
     CDS             complement(1845..3056)
                     /locus_tag="PK399_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HPW13935.1"
                     /translation="MDGTPSARLLAQAPHRLMFFIGASNLLLAMAWWAMWLADARWQL
                     FGLRQPVPYAGWLHAFVMQYQVLPSFIFGFLLTVFPRWMGLPELERWRYLPVGLGIFG
                     GQLATLAGMFGFEPGVVVGYWMTLAGWLACMATLGPLLTRERGTTWHARACFLGLSMG
                     LLGLLAWGGFLLGGDARLAFASIKLGTFGLLLPVYFSVAHRMFPFFAGNVVPGYVAWR
                     PLWMLGAVIALCMAHLGLELSHAYPWLWLADLPLFALLSVALWTWWPRGPAPGLLQVL
                     FLGLAWLPLAIGLYAFQSLAYSLTGDFLLGRAPAHALFIGFFGSVLVAMVTRVTQGHS
                     GRALVMPAAAWFAFAAIQLVALARVFAEVVPDGLAWQAVAAIGWLLAFAPWVLRIGRI
                     YLSPRTDGKPG"
     gene            complement(3061..3297)
                     /locus_tag="PK399_12265"
     CDS             complement(3061..3297)
                     /locus_tag="PK399_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13936.1"
                     /translation="MQYTLPTVIPPDLATIEQALAMYDPSALVDYDARHGSLRISTVA
                     TEPELLASLRGAGIAAAAERLQRVPSQCCGGCGG"
     gene            complement(3327..>3709)
                     /locus_tag="PK399_12270"
     CDS             complement(3327..>3709)
                     /locus_tag="PK399_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13937.1"
                     /translation="SARTRFPEHLPGRLLFALRGLRGQMVWDTRALQVSFWCLNIGLA
                     LMAVLTLLPLGTMQLLAAIEHGYAYARSAEFMQKPIVEMLVWMRVPGDTIFSVGALAL
                     CWFVLRLWIAPRRDPAFSGSAEHE"
BASE COUNT          655 a         1296 c         1304 g          454 t
ORIGIN      
        1 tgggaacacc ttggcggcga tggccggcat tcgctgcagg cgccgctggc caccctgcat
       61 gcgttcaacg gctgggccga caagttcctg gtcacgcccg cggcgggact ggacgatcgc
      121 tacctcggcc tgggcggcaa cctcggccgc gaacgcgcgg gcacccgcac cgcctggcaa
      181 ctggcctggc acgactaccg cgccgaccgc ggcggcctgc gttacggaag cgagtggaac
      241 gcatcgctca ccgtgccgct ggcgaaggga ctgggcgcca ccgtcaagct ggccgactac
      301 cgcgcatccg ccttcgcccg cgacacccgc aagctctggc tgcaggtcga gtacaagggg
      361 cggcgttgac gccgctgcgt caggcggctt gcaggcgcgc ggccaggcgc gcatgcgcgg
      421 gaccggggtc gatggcgtgc gcggccagcc agtcgtggtc gaacagggtc tgcgcgtagc
      481 ggtcgccgcg atcgcagagc agggtgacga tgctgccgtt gcagccctgt tcgcgcatgc
      541 cttcggcaag ctggaggcag gccatcaggt tggttcccga cgagccgccg taacgccggc
      601 ccagcagttc ttccaggcgc cacgccgcgg cgatggagtc ggcatcttcc acctcgacca
      661 ccgcatccac cacgtcgaag atgaagccgg gctccacccg gggtcggccg atgccttcga
      721 tcaacgtggg atgcgttgcc gttgcatgcg ggtcgcgccc gcgccagccc cgcacgaagc
      781 cgctgccgac cggttccgcc acgcacaggc gcgtgtccag cccgcgatag cgcaggtagc
      841 gcccgatggt ggcggaggta ccgccggtgc cgaccccgca gacgatccac gcgggatgcg
      901 gacatggttc gcgggccatc tggtcgagga tggattcggc gatgttgttg ttgccgcgcc
      961 agtcggtggc gcgttcggcc aggccgaact ggtcgaggaa gcaggcgccg cccccggcga
     1021 tcgcgcgtgc gcgcgactgg gtgcattcgc catccgcggt gagttcgcag tgcccgccga
     1081 gggagcgcac cgcctcgatc ttgccgggcg ccgtgcaggc cggcatcacc gcgatgaacc
     1141 gcaggccgag caacctggcg aaccaggctt cggaaatcgc ggtgctgccg gaggaggcgt
     1201 ccaccaccgc ctggccttcg cgcaggcggc cgttgcacag cgcgtacagg aacagcgagc
     1261 gcgccagccg atgcttgagg ctgccggtgg gatgcgcggc ttcgtccttg aaatagaagt
     1321 cgatgccggg gaacccggcg aatcgcatgt gcagcaggtg ggtatcggcg gaccgcgcgg
     1381 cttcgcggcg caactgcagg atcgcgtcag ccgcccagcg gcgctccccc gcgttccaat
     1441 cgtcgttcat gcgagccagc gcgccagcca gcccagcgga tgatgcgcgc gggcgatgcc
     1501 gtagatcgcg gcgaacgcca gcagcgccga cgcgtagtac agggcgcggg cgcgccccga
     1561 gcgatgcggg cgcatcgcca gcacgccaag caccacgtag gcgacgatca gcaggatttt
     1621 ggcgagcagc cagccgttgg cgaacatcgc ccagggcagg atggtcagca gcatcatcgc
     1681 cgcggtcagc aggctggtgt cgatggcgta gctcaaccat ttcaccggcc atgcccgcgg
     1741 ccagcgcgca cccgccagcg cgaagccgcc gcgcagcgcg aacagggcgc cgctgagcag
     1801 cgcgatgacg atgtggaact gcttgatctg cgggtagaac gcgatcatcc gggcttgccg
     1861 tccgtgcggg gcgagagata gatccggccg atccgcagca cccaaggggc gaatgccagc
     1921 agccagccga tcgccgccac tgcctgccag gcgagtccgt ccggcacgac ctcggcgaac
     1981 acccgcgcca gtgccaccag ctggatcgcc gcgaacgcga accacgcggc cgccggcatt
     2041 accagcgcgc gcccggaatg gccctgggtg acgcgggtga ccatcgccac cagcacgctg
     2101 ccgaagaagc cgatgaacag cgcatgcgcc ggtgcgcgac cgagcaggaa gtcgccggtg
     2161 agcgaatacg ccaggctctg gaacgcatac aggccgatcg ccagcggcag ccaggccagg
     2221 ccgaggaaca acacctgcag caggccgggg gccgggccac gcggccacca cgtccacagc
     2281 gcgaccgaca gcagtgcgaa cagcggcagg tcggccaacc acagccatgg gtaggcgtgc
     2341 gacaactcca ggcccaggtg ggccatgcaa agcgcgatga ccgcgcccag catccacaac
     2401 ggtcgccagg ccacgtaacc gggtaccacg ttgccggcga agaacgggaa catccggtgc
     2461 gcgacgctga agtacaccgg caggagcagc ccgaacgtgc cgagcttgat gctggcgaac
     2521 gcgagcctgg cgtcgccgcc cagcaggaag cccccccatg ccagcaggcc gagcaggccc
     2581 atcgacaggc ccaggaagca ggcgcgcgca tgccaggtgg tgccgcgctc gcgcgtgagc
     2641 agcggcccga gcgtggccat gcaggccagc cagccggcga gggtcatcca gtagccgacc
     2701 accacgccgg gctcgaagcc gaacatcccg gccagcgtcg ccagttgtcc gccgaagatg
     2761 cccagcccca ccggcaggta gcgccagcgc tccagttccg gcaggcccat ccagcgtggg
     2821 aacacggtga gcaggaagcc gaagatgaag ctgggcagca cctggtactg catcacgaac
     2881 gcatgcagcc aaccggcata gggcacgggc tggcgcaggc cgaacagctg ccagcgtgca
     2941 tccgccagcc acatcgccca ccacgccatc gccagcagca ggttgctggc gccgatgaag
     3001 aacatcagcc ggtgcggtgc ctgcgcgagc aggcgcgcgg agggggtgcc gtccatcctg
     3061 tcagccgccg caaccgccgc agcattgcga aggaacgcgc tgcaggcgct ccgccgcggc
     3121 ggcgatgccg gctccgcgca gcgacgcgag cagctccggc tccgtcgcca cggtcgagat
     3181 gcggaggctg ccatggcgtg cgtcgtagtc gaccagcgcc gagggatcgt acatggccag
     3241 ggcctgttcg atcgtcgcga ggtcaggcgg gatcacggtg ggaagggtgt actgcatgga
     3301 agcatccggt gggtggcgag gcggtgtcat tcgtgttcgg cggatccgct gaaggccggg
     3361 tcgcgcctgg gcgcgatcca caaccgcagc acgaaccagc acagcgccag cgcgccgacg
     3421 ctgaagatcg tgtcgccggg cacgcgcatc cacaccagca tctccacgat cggcttctgc
     3481 atgaactccg ccgagcgggc gtaggcatag ccatgctcga tcgccgccag cagctgcatg
     3541 gtgcccagcg gcagcagggt cagcacggcc atcagcgcca ggccgatgtt gaggcaccag
     3601 aacgagacct ggagcgcgcg cgtatcccac accatctgcc cacgcagtcc gcgcagggcg
     3661 aacagcagcc gaccgggcag gtgctcgggg aagcgcgtcc gtgccgatc
//
LOCUS       DAOOCX010000198         3677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_24069_length_3677_cov_7.101601, whole genome shotgun sequence.
ACCESSION   DAOOCX010000198 DAOOCX010000000
VERSION     DAOOCX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24069_length_3677_cov_7.101601"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..368
                     /locus_tag="PK399_12275"
     CDS             183..368
                     /locus_tag="PK399_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA binding domain-containing
                     protein"
                     /protein_id="HPW13938.1"
                     /translation="MSEKKAYPLRISAEILGATQRWADDELRSVNAQIEYLLREALRK
                     AGRLPKPRRPPSEENDT"
     gene            365..532
                     /locus_tag="PK399_12280"
     CDS             365..532
                     /locus_tag="PK399_12280"
                     /inference="COORDINATES: protein motif:HMM:NF025156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HPW13939.1"
                     /translation="MSTRWNYKVVEVTQWMGTKPKKIEETIAPLGQLGWELVAVTQVA
                     ATSILYFKKPA"
     gene            568..1965
                     /locus_tag="PK399_12285"
     CDS             568..1965
                     /locus_tag="PK399_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPW13940.1"
                     /translation="MLHIVHMIERRFGVERASHIVIRLLLASALLLMLGLGLLLSGRV
                     VAQEAPIDGAAIATRALDHLDAGEYAQVEALFSADMAKAVPADKLKGVWESLPAQVGK
                     PTGRGEAQSMLQDGATLVQVPLHFEKAELVAKFAIDVSGKVVGFMIQPAQAAAPAPAA
                     AVRDDANFSERELAVGEDERALPGTLAMPKGGGPLPAVVLVHGSGPHDRDETIGPNKP
                     FLDIARGLATQGIAVLRYEKRTRARPQDFASGVFGVDEETTNDAVQAVAALRALDGID
                     PKRVFVLGHSQGGMMAPRIAAVSGHVAGLVLLAAPARSLLDIVIEQNRRLAVLDDQKT
                     SDAERDAITALIQQVRSIRDPATDAATKTVMGLPAGYWRSIDSVDPVAEAKSVALPML
                     VLQGARDIQVVDADWQAWRSGFHDNPKVTFKLYETLNHLAMPGEGEGNLAEYQQPNHV
                     DPALITDVAAWIKAH"
     gene            1968..2507
                     /locus_tag="PK399_12290"
     CDS             1968..2507
                     /locus_tag="PK399_12290"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPW13941.1"
                     /translation="MKTAIAPPREFPLAPLQVKAWLFMVVLAIALVAFVILIPARPDQ
                     VGPFWQALPIAIIVIAIVLPLAMLRRRRVTLQDGQLVVAATFYTRKLPLAALDLDHAR
                     IVDLGEHNEYKPLLKTNGYALPGFAAGHFRLRNRAKAFCLLTDQERVLILPQRDGAFL
                     LLSPERPQVLLDALKAARG"
     gene            2539..3354
                     /locus_tag="PK399_12295"
     CDS             2539..3354
                     /locus_tag="PK399_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPW13942.1"
                     /translation="MTRTPPVPLKAVLLNTPDIELWPGGLLRARSNGDARILSRARRV
                     LRRKRDGTYLAADLAEGIQPLVPRLHREPGLDAALDLLDASPLHARNGIDHVGQLPLH
                     RLQDRLDALGVDAEGYAQRTGLGLVAEPDWLAFAGFDRYRRALWLRIDAARAWLRMRE
                     AAVRDGIVLEAISGYRSHDYQLGIFVRKLARGLSVDEILRVNAAPGFSEHHGGCALDI
                     GTPDEPPAEESFEATPAFGWLQANADGHGFAMSYPRGNPRGIVYEPWHWRFAG"
     gene            3360..>3677
                     /locus_tag="PK399_12300"
     CDS             3360..>3677
                     /locus_tag="PK399_12300"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPW13943.1"
                     /translation="MPPWLRGPTLADAWASGRNNFNLIRLLAAWLVIYGHAWAITGSA
                     GGDLVTQLTQFKFAGGVAVDVFFFISGFLIAASLERNDTRGYLASRALRILPALVVCV
                     ALSV"
BASE COUNT          589 a         1292 c         1226 g          570 t
ORIGIN      
        1 tggtcagcaa cctgctggtg gtcctgtgcg gcgagcgcgg cacccagccg atcgtcaacg
       61 ccggcagcct gtcctgatcc cggccgatcg cagaccgatg gccaccccct cgatcatcgc
      121 ccccgtcgca gtcgccggca gccgcgtgct gccggtgcgc gacccgcact gagccggccg
      181 gcatgagcga gaagaaggcc tatccgctgc gcatcagcgc cgagatcctc ggtgccaccc
      241 agcgctgggc cgacgacgag ctgcgcagcg tcaacgccca gatcgaatac ctgctgcgcg
      301 aggcactgcg caaggccggc cgcctgccga agccgcgccg gccaccatcc gaggagaacg
      361 acacgtgagc acgcgctgga actacaaggt ggtcgaggtc acccagtgga tgggcacgaa
      421 gcccaagaag atcgaggaaa cgatcgcgcc tctcggccaa ttgggctggg agttggtcgc
      481 agtgacccag gtcgccgcca ccagcatcct ttatttcaag aagccggcct gagcgccgcg
      541 ctccgcactg cgtcctggga ggacaccatg ctccacatcg tccacatgat cgaacgccgg
      601 ttcggcgtag agcgcgcatc gcacatcgtc atccgcttgc tgctggcgag cgcgctgctg
      661 ctgatgctgg gactgggcct gttgctgtcc ggccgcgtcg ttgcgcaaga ggcgccgatc
      721 gatggcgcag cgatcgccac ccgcgcgctc gaccacctgg atgccggcga atacgcgcag
      781 gtcgaggcgc tgttctccgc cgacatggcc aaggcggtgc cggccgacaa gctgaaaggc
      841 gtgtgggaat cgctgccggc gcaggtcggc aagcccaccg gtcgcggcga ggcgcaatcg
      901 atgctgcagg acggcgccac gctggtgcag gtgccgctgc atttcgagaa agccgaactc
      961 gtggcgaaat tcgccatcga cgtctccggc aaggtcgtgg gcttcatgat ccagccggcc
     1021 caggccgccg cgccggcacc cgcggcagcg gtgcgggacg acgcgaactt cagcgaacgc
     1081 gagctcgcgg ttggcgagga cgagcgtgcc ctgcccggca cgctggcgat gccgaagggc
     1141 ggtggcccgt taccggcggt ggtgctggtg catggctccg gcccgcacga tcgcgacgaa
     1201 acgatcggcc cgaacaagcc cttcctcgac atcgcccgcg gcctcgccac gcagggcatc
     1261 gcggtgctgc gctacgagaa gcgcaccagg gcgcgtccgc aggacttcgc cagcggcgtc
     1321 ttcggcgtcg acgaggagac caccaacgat gcggtgcagg cggtcgccgc gctgcgcgca
     1381 ctcgacggca tcgaccccaa gcgcgtgttc gtgctcggcc acagccaggg cgggatgatg
     1441 gcgccgcgga tcgccgcggt ttccggccac gtcgccggct tggtgctgtt ggccgccccg
     1501 gcgcgctcgc tgctcgacat cgtcatcgaa cagaaccgcc ggctggcggt gctggacgac
     1561 cagaagacca gcgatgccga acgcgacgcg atcaccgcgc tgatccagca ggtccgcagc
     1621 atccgcgacc ccgccaccga cgccgccacc aagaccgtca tgggcctgcc cgccggctac
     1681 tggcgcagca tcgacagcgt cgatccggtc gccgaagcga aatccgtagc actgccgatg
     1741 ctggtactgc agggcgcgcg cgacatccag gtggtcgatg ccgactggca ggcatggcgc
     1801 agtgggttcc acgacaatcc gaaggtgacg ttcaagctct acgaaacgct caaccacttg
     1861 gcgatgcccg gtgagggaga gggcaacctg gccgagtacc agcagcccaa ccacgtcgat
     1921 ccggcgctga tcaccgacgt ggctgcctgg atcaaggcgc actgacagtg aaaacggcaa
     1981 tcgccccgcc gcgcgagttt ccgcttgcgc cgctgcaggt gaaggcatgg ctgttcatgg
     2041 tcgtgctggc catcgccctg gtcgcgttcg tcatcctcat ccccgcacgc cccgaccagg
     2101 tcgggccgtt ctggcaggcg ctgccgatcg cgatcatcgt catcgcgatc gtgctgccgc
     2161 tggcgatgct caggcgcagg cgcgtcacgt tgcaggatgg ccaactggtg gtcgcggcca
     2221 cgttctacac gcgcaagctg ccgctggccg cactcgatct cgaccatgcc cgcatcgtcg
     2281 acctcggcga acacaacgag tacaagccgt tgctgaagac gaatggctat gccctgcccg
     2341 gtttcgccgc agggcacttc cgcctgcgca accgcgcgaa ggcgttctgc ctgctcaccg
     2401 accaggagcg cgtgctgatc ctgccgcagc gcgatggcgc cttcctgctg ctcagccccg
     2461 agcggccgca ggtgttgctg gacgcactca aggccgcgcg cggctgacgc caacgctgcc
     2521 aggttctatc ctgcgccgat gacacgcacg ccaccggttc cgctgaaggc cgtcctgctc
     2581 aacacgcccg acatcgagtt gtggcccggc ggcctgttgc gcgcgcgcag caacggcgat
     2641 gcccgcatcc tgtcgcgtgc gcgccgcgtg cttcgacgca agcgcgacgg cacctacctg
     2701 gcggccgatc ttgcggaagg gatccagccg ctggtgccgc gcctgcatcg cgaacccggc
     2761 ctggacgctg cgctggacct gctggacgcg tcgccgctgc acgcacgcaa cggcatcgat
     2821 catgtcggcc aactgccgtt gcatcgactg caggaccggc tggatgcgct cggcgtcgat
     2881 gccgagggct acgcgcaacg aaccgggctg ggtctcgttg ccgaacccga ctggctcgcg
     2941 tttgcgggct tcgaccgcta ccggcgcgcg ttgtggctgc gcatcgatgc cgcccgcgcg
     3001 tggttgcgca tgcgcgaggc ggcagtgcgc gatggcatcg tgctggaggc gatctccggc
     3061 taccgcagcc acgactacca gctgggcata ttcgtgcgga agctcgcacg cggtctgtcg
     3121 gtcgacgaga tcctgcgggt caatgccgcg cccggttttt ccgaacacca cggcggttgc
     3181 gcactggaca tcggcacgcc cgacgagccg ccggccgaag aatcgttcga ggccacgccc
     3241 gccttcggct ggttgcaggc gaacgccgat ggacacggct tcgcgatgag ctatccgcgc
     3301 ggcaatccgc gcgggatcgt ttacgaacct tggcactggc gcttcgcagg ctgatgacga
     3361 tgccgccatg gcttcgcggc ccgaccctcg ccgacgcctg ggccagtggc cgcaacaact
     3421 tcaacctgat tcgccttttg gccgcatggc tggtgatcta cggccatgcc tgggcgatca
     3481 ccggcagcgc cggcggcgac ctcgtcaccc agctgacgca attcaagttc gccggcggcg
     3541 tcgcggtgga cgtgttcttc ttcatcagcg gattcctgat cgcggccagc ctcgagcgaa
     3601 acgacacccg cggctacctg gcctcgcgcg cgttgcggat cctgccggcg ctggtggtgt
     3661 gcgtggcgct gagcgtg
//
LOCUS       DAOOCX010000199         3623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_24614_length_3623_cov_7.177971, whole genome shotgun sequence.
ACCESSION   DAOOCX010000199 DAOOCX010000000
VERSION     DAOOCX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24614_length_3623_cov_7.177971"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1126
                     /locus_tag="PK399_12305"
     CDS             125..1126
                     /locus_tag="PK399_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020850886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPW13944.1"
                     /translation="MHDSAAPAPRRLALPAIQALAASEMAAMDALIRQRLASDVVLIN
                     QVAEYIIGAGGKRLRPMLLLLTAGALGHRSADAHQLAAVVEFIHTSTLLHDDVVDESD
                     LRRGRKTANAVWGNAASVLVGDFLYSRSFQLMVELERMDVQQLLANTTNTIAEGEVLQ
                     LLHVRNPDTDQAAYLRVIERKTAVLFAAATRLGALLAGADAATCDAMHRYGMALGYAF
                     QIADDVLDYASDAGTLGKNLGDDLAEGKATLPLIHAMQHSGAPTQAILRTAIQQGDTG
                     ALPQVMAAIHACGSLDYSRDCALQYARDAEAALAGLDDNEFVAALRGLAQYSVSRDH"
     gene            complement(1156..1971)
                     /locus_tag="PK399_12310"
     CDS             complement(1156..1971)
                     /locus_tag="PK399_12310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015463439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPW13945.1"
                     /translation="MRRPPLNRLLLALGLLLATGPAFAAMQAKPIEWKVGKERFSGYV
                     VYDDASTDKRPGLVMVPNWMGVTKDSVERAKAIAGDDYVILIADVYGKGNRPKDAKQA
                     GALAGSLRGDDRTRLRARMQGALATLKAQAGKAPLDADRIGAFGFCFGGSAVLELARG
                     GDELAGVVSLHGGLAPGVKSATAEAVKTPLLVLNGADDKAVPDSDILAFEKEMDGAGA
                     DWQFVDFSGAVHCFAEPSAGNDPSTNCAYDERAAKRAYRMMDDFFEERFGAAD"
     gene            complement(2091..3488)
                     /gene="murD"
                     /locus_tag="PK399_12315"
     CDS             complement(2091..3488)
                     /gene="murD"
                     /locus_tag="PK399_12315"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPW13946.1"
                     /translation="MKIADLAGQRVALWGWGREGRAAYTALRARLPEQALTLFCSEAE
                     VADAAASHDPLLVIEHAATGERLGAFDVVVKSPGISPNKPEALEAASRGTRFIGGTAL
                     WFGERADAAGDVANAICITGTKGKSTTSSLLAHLLRASGLRTALAGNIGLPLLELLDE
                     DADAWAIELSSYQTRDVAASGARPRIAIVTNLHPEHLDWHGSETRYVEDKLALVTEAH
                     PHIAVLNAADPRLAALVLPGSEIRWYGDTRGWHLRGDALYRGDAFVMQTESMPLPGWH
                     NRSNLCAVLTALDAFGLDAAALAPHAATFQPLPNRLQPIGTRDGILYVNDSISTTPMA
                     TLAALSLYRDRPVALLVGGHDRGLPWDDFAHAMHAQAPRAIVTMGQNGPRIRALLEPV
                     AAQAGFALSSATDLADAMAQARDALPDGGVVLLSPGAPSFGVYRDYVARGRHFAELAG
                     FDPEMISGISGMGIV"
BASE COUNT          596 a         1271 c         1208 g          548 t
ORIGIN      
        1 cgcgacgagg tgcagaagcg catgtcgcag accgcctcgg aggcgatgct gcccggatag
       61 gcgggtggcg ggaccggtcg gggcggcgcg gcctataatt gcgcatcccc cgacgcgaca
      121 tcccatgcac gactccgccg cgccagcccc acgtcgactc gccctgcccg cgatccaggc
      181 cctggccgca tcggagatgg cggcgatgga tgcgctgatc cgccagcggc tggcctcgga
      241 cgtggtgctg atcaaccagg tggccgaata catcatcggc gccggcggca agcggctgcg
      301 gccgatgctg ttgttgctga ccgcgggtgc gctggggcat cgcagcgccg acgcccacca
      361 actggcggcg gtggtcgaat tcatccacac ctccaccctg ctccacgacg acgtggtcga
      421 cgagtccgac ctgcgccgcg gccgcaagac cgccaatgct gtgtggggca atgccgccag
      481 cgtgctggtc ggcgacttcc tgtattcgcg cagcttccag ttgatggtcg agctggaacg
      541 catggacgtg cagcagttgc tggccaacac caccaacacc atcgccgagg gcgaggtgtt
      601 gcagctattg cacgtgcgca acccggatac cgaccaggcc gcctacctgc gggtcatcga
      661 gcgcaagacc gccgtgctgt tcgccgcggc gacgcgcctt ggtgcattgc tcgccggtgc
      721 cgatgccgcc acctgcgacg ccatgcatcg ctacggcatg gcgctgggct acgccttcca
      781 gatcgccgac gacgtgctgg actacgcctc cgatgcggga accctgggca agaacctcgg
      841 cgacgacctg gccgaaggca aggccacgct gccgctgatc catgcgatgc aacacagcgg
      901 cgccccgacg caagccatcc tgcgcacggc gatccagcag ggcgatacag gtgcactgcc
      961 gcaggtcatg gccgcgatcc acgcctgcgg cagcctggac tacagccgcg actgcgcgct
     1021 gcagtacgca cgcgacgcag aggctgcgct ggcgggcctg gacgacaacg agttcgtcgc
     1081 ggccctgcgt ggcctcgccc agtactcggt cagccgcgac cactgagtcg cggtcgaccc
     1141 gcgatcgacc cgcgatcagt cggccgcgcc gaagcgctcc tcgaagaagt cgtccatcat
     1201 ccggtaggcg cgcttggccg cgcgttcgtc gtaggcgcaa ttggtggatg gatcgttgcc
     1261 ggcgctcggc tcggcgaagc aatgcacggc gccactgaag tcgacgaact gccaatccgc
     1321 gccggcgccg tccatctctt tctcgaatgc aaggatgtcg ctgtccggca ccgctttgtc
     1381 gtcggcaccg ttgagcacca gcagcggggt cttcacggcc tcggcggtcg ccgacttgac
     1441 gccaggggcc agcccaccat gcaggctgac cacgccggcg agttcatcgc caccccgcgc
     1501 cagttccagg accgcggagc cgccgaaaca gaagccgaat gcaccgatcc tgtcggcatc
     1561 caatggcgcc ttgccagcct gcgccttgag cgtggccagt gcgccctgca tgcgcgcgcg
     1621 caggcgggtg cgatcatcgc cgcgcaaact gcccgcaagc gcgccggcct gcttggcgtc
     1681 cttgggccgg ttgcccttgc cgtacacatc ggcgatcagg atcacgtagt catcgccggc
     1741 gatggccttc gcgcgttcga ccgagtcctt ggtgacgccc atccagttcg gcaccatcac
     1801 caggccgggg cgcttgtccg tcgacgcatc gtcgtagacc acgtagccgc tgaagcgctc
     1861 cttgccgacc ttccattcga tcggtttcgc ctgcatcgcg gcgaacgcgg ggccggtggc
     1921 cagcagcaat ccgagggcaa gcagcagtct gttcaagggc gggcggcgca tggcgggatc
     1981 tccgggggac gtctgcctac gctaacccgg ccgggttcaa catgcgtgaa cttccattcc
     2041 cgtcattccc gcgaaggcgg gaatgacaag caatagcagg ctgacgagcc tcaaacgatc
     2101 cccatccccg agatcccgga aatcatttcc ggatcgaatc cggccagttc ggcgaagtgg
     2161 cggccgcgcg ccacgtagtc gcggtacacg ccaaagctcg gcgctcccgg cgacagcagg
     2221 accacgccgc catcgggcag tgcgtcgcgt gcctgcgcca tcgcatcggc aaggtcggtg
     2281 gccgacgaca gcgcgaaccc cgcctgtgcc gccaccggct ccagcaacgc gcgaatgcgc
     2341 gggccgttct gccccatggt cacgatcgcg cgcggcgcct gcgcgtgcat cgcatgggcg
     2401 aagtcgtccc acggcaggcc acgatcatgc ccgcccacca gcaaggcgac cgggcggtcg
     2461 cgatacagcg acagcgccgc cagcgtggcc atcggcgtgg tgctgatgga atcgttgaca
     2521 tagaggatgc cgtcgcgcgt gccgatgggt tgcagccggt tgggcaaggg ctggaacgtg
     2581 gccgcatgcg gcgccagagc ggcggcatcc agcccgaacg cgtccagcgc ggtgagcacg
     2641 gcgcacaggt tgctgcggtt gtgccagccc ggcagcggca tggactcggt ctgcatcacg
     2701 aatgcgtcgc cgcgatacag cgcgtcgccc cgcaggtgcc aaccgcgcgt atcgccgtac
     2761 cagcggatct cgctgccggg cagcaccagc gcagccaagc gcggatcggc ggcattgagc
     2821 acggcgatgt gcggatgcgc ctcggtcacc agcgccagct tgtcttcgac gtagcgcgtc
     2881 tcgctgccgt gccagtccag gtgttcggga tgcaggttgg tgacgatggc gatgcgcggg
     2941 cgcgcgccgg aggcggccac gtcccgggtc tggtagctgg acaactcgat tgcccacgcg
     3001 tcggcatctt catccaggag ttccagcaac ggcaggccga tattcccggc cagcgcggtg
     3061 cgcaggcccg acgcgcgcaa caggtgcgcc agcaatgacg aggtggtgct cttgcccttg
     3121 gtaccggtga tgcaaatcgc gttggcgaca tcgccggcgg cgtcggcgcg ctcgccgaac
     3181 cacagcgcgg tgccgccgat gaagcgggtg ccacgcgatg ccgcttccag cgcttcgggc
     3241 ttgttcggac tgatcccggg cgacttcacc accacgtcga acgcaccaag gcgttcgccg
     3301 gtcgcggcat gctcgatcac cagcaacgga tcatgcgacg ccgcggcatc ggcgacttcg
     3361 gcctcgctgc agaacagcgt cagcgcctgc tcgggcaagc gcgcgcgcaa tgcggtgtag
     3421 gccgcgcgac cctcgcgccc ccaaccccac agggcgacac gctggccggc gaggtcggcg
     3481 atcttcatcg ggaatcagtc agcgaaacgc agacgcagcc gatcccagag cgcagccggg
     3541 atgcgctgtt cctcgtccaa ctcgagcaat ggctcgatgc gcaggtcttc cgcctgcaac
     3601 tgcggcgcga tctcgcgggc gaa
//
LOCUS       DAOOCX010000200         3602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_24834_length_3602_cov_5.906682, whole genome shotgun sequence.
ACCESSION   DAOOCX010000200 DAOOCX010000000
VERSION     DAOOCX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24834_length_3602_cov_5.906682"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1745
                     /locus_tag="PK399_12320"
     CDS             <1..1745
                     /locus_tag="PK399_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW13947.1"
                     /translation="LEFPYSVDDRDQMRKPRRVASGLTEYVLRTGKPLLADRARIRRL
                     ESEGKVRSIGTLAHCWLGVPLLYDDKVVGVIAVQSYSPDVAFTTRDQELLTFVAYHIG
                     SSLARKQAQDRLMHAHAGLEQRVSERTRELAEANAELVEQIGERMRAERKLTYQATHD
                     ALTGLPNRTQLLERLGKAIAVARGNPQACFAVLFLDLDRFKLVNDSAGHAVGDELLIE
                     AARRIVGSVRTGDVVSRLGGDEFAILAESLDGPDMAAELGRRVLAALGAPVWLAGREL
                     FPTASIGIALSHARYESGEDMLRDADAAMYRAKGDGRDRSVMFDEHMRDEATRILDME
                     SDLRRAINAEAFEPFYQPIVQLVSGERVGHEALLRWRHEQRGLLTPGDFLDVGEDSGL
                     IEQVDWLMYARVIADMARYPMPGYVAINVSPRHFRAPDFASRLLDLLDAAQVAPSRLR
                     IEITEVALLDDAPRTSESLNMLRSHGVLAQLDDFGTGFSALSYLHRFPIASLKIDRSF
                     VSGLDGDVRTESVAVIRAIVALANSLNIEIIAEGVETQRQRERLHDLGCMYAQGYLFG
                     RPAPPVPYVAVQSG"
     gene            complement(1764..3191)
                     /locus_tag="PK399_12325"
     CDS             complement(1764..3191)
                     /locus_tag="PK399_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW13948.1"
                     /translation="MMKKRWPYYLANQPVQANTRLDVLDKFSGERVARVAFADAAAIE
                     DAIAAAYAAREAMAAFPPDARRDVLEHCVARFEARSEELALALCIEAGKPIKDARGEV
                     TRLVDTFRIAAGEATRIGGEVLELQLSARTRGYRGMTKRVPIGACSFITPFNFPLNLV
                     AHKVAPAIAAGCPFVLKPAAKTPLGALIIGEVLAETDLPTGAFSIMPCSNDDASALVE
                     DERIALLSFTGGLVGWDMKARAGRKKVTLELGGNAACIVDADPGRPLDEAVERLVFGA
                     YYQSGQSCISVQRILVHRDLHDALAKKLVTAVKKLKAGDPRDEATSIGPVIDEASAKR
                     IDDWIADAIRGGAKALVRGKRTGNLLGASLLRDVPRDADLYRKEAFGPVACIEAYDDF
                     DQAIARVNDSDFGLQAGVLTARIDHAMRAWDRLEVGGVVVGDVPSFRVDNMPYGGIKQ
                     SGLGREGVRCAIEDMTEPRLLVIRD"
     gene            complement(3185..>3602)
                     /locus_tag="PK399_12330"
     CDS             complement(3185..>3602)
                     /locus_tag="PK399_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPW13949.1"
                     /translation="LDALARRGEQAQLRLANALQRRLEQERARLRHADAVLRAAHPRR
                     RIDRLRERLQALRGRPQSLLARHLQHEALRLRGLARSLHAISPLATVARGYSILQRED
                     GRVVRGIGDTHPGEALQARLHDGRLRLRVEGTESGS"
BASE COUNT          535 a         1246 c         1270 g          551 t
ORIGIN      
        1 tgctggagtt cccgtattcg gtggacgacc gcgaccagat gcgcaagccg cgccgagtcg
       61 ccagcggcct gaccgaatac gtgttgcgga ccggcaagcc gctgctggcc gaccgtgcgc
      121 ggatccgccg gctggagtct gaaggcaagg tccgaagcat cgggaccctg gcgcactgct
      181 ggctgggggt gccgttgctg tacgacgaca aggtggtcgg cgtgatcgcc gtgcagagct
      241 attcgccgga cgtggcgttc accacccgcg accaggaact gctgacgttc gtggcctacc
      301 acatcggcag cagcctggcc cgcaaacagg cgcaggaccg gctgatgcac gcgcacgccg
      361 ggctggaaca acgggtcagc gagcgcaccc gcgaactggc cgaggccaat gccgagctgg
      421 tcgaacagat cggcgagcgc atgcgcgccg agcgaaagct gacctaccag gccacccacg
      481 acgcgttgac cggattgccc aaccgcaccc agttgctgga acgcctgggc aaggcgatcg
      541 cggtggcgcg cggcaacccg caggcctgct tcgcggtgct gttcctcgac ctcgaccggt
      601 tcaagctggt caacgacagc gccggccacg cggtcggcga cgaactgctg atcgaggccg
      661 cccgccgcat cgtcggcagc gtgcgtaccg gcgacgtggt ctcgcggctg ggcggcgatg
      721 aattcgcgat cctggcggaa agcctggacg ggccggacat ggccgccgag ctcggtcgtc
      781 gcgtgctggc cgcgctgggt gcgccggtgt ggctggcggg gcgcgaactg ttcccgaccg
      841 ccagtatcgg catcgccctg tcgcacgcgc gttacgagag cggcgaggac atgctgcgcg
      901 acgccgatgc cgcgatgtac cgcgccaagg gcgacggccg cgaccgctcg gtgatgttcg
      961 acgagcacat gcgcgacgaa gccacccgca tcctcgacat ggaatccgac ctgcgccgcg
     1021 cgatcaacgc cgaggcgttc gagccgttct accagccgat cgtgcaactg gtcagcggcg
     1081 agcgcgtcgg ccacgaggcg ctgctgcgct ggcgccatga gcagcgcggc ctgctcaccc
     1141 ccggcgattt cctcgacgtg ggcgaggaca gcggcttgat cgaacaggtg gactggctga
     1201 tgtatgcgcg ggtcatcgcc gacatggccc gctatccgat gccgggctac gtcgcgatca
     1261 acgtgtcgcc gcggcacttc cgcgcgcccg acttcgcgag ccggctgctg gacctgctgg
     1321 atgccgccca ggtggcaccg tcgcggctgc gcatcgagat caccgaggtc gcgctgctcg
     1381 acgacgcccc gcgcaccagc gaaagcctca acatgctgcg ttcgcatggc gtgctggcgc
     1441 agctggacga cttcggcaca ggcttctccg ccctgtcgta cctgcaccgg ttcccgatcg
     1501 ccagcctgaa gatcgaccgc agcttcgtct ccggcctgga cggcgatgtc cgtaccgaga
     1561 gcgtggcggt gatccgagcg atcgtggcgc tggccaacag cctgaacatc gagatcatcg
     1621 ccgagggcgt ggagacgcag cggcagcgcg aacgcctgca cgacctgggt tgcatgtacg
     1681 cgcagggcta cctgttcggc cgaccggcgc cgccggtccc gtacgtggcc gtgcagtcgg
     1741 gctgacgggc tgagcgccgg ggttcagtcg cggatgacca gcaggcgcgg ctcggtcatg
     1801 tcctcgatcg cgcagcgcac gccctcccgg ccaagcccgg actgcttgat cccgccgtag
     1861 ggcatgttgt cgacgcggaa gctgggcacg tcgccgacca ccacgccgcc gacctccagc
     1921 cggtcccagg cgcgcatggc atggtcgatg cgcgcggtga gcacgccggc ctgcaggccg
     1981 aagtcgctgt cgttgacccg ggcgatggcc tggtcgaagt cgtcgtacgc ctcgatgcag
     2041 gcgaccgggc cgaacgcttc cttgcggtag aggtcggcgt cgcgcggcac gtcccgcaac
     2101 agcgaggcac cgagcaggtt gcccgtgcgt ttgccgcgca ccagcgcttt cgcgccgccg
     2161 cggatggcgt cggcgatcca gtcgtcgatg cgtttggcgg acgcttcgtc gatgaccggt
     2221 ccaatggagg tggcctcgtc gcgcggatcg ccggccttga gcttcttcac cgcggtaacc
     2281 agtttcttcg ccagcgcatc gtgcaggtcg cgatggacca ggatgcgctg cacgctgatg
     2341 caactctggc cgctctggta atacgcaccg aacaccagcc gttcgaccgc ctcgtcaagc
     2401 ggcctgccgg gatcggcatc gacgatgcag gcggcattgc cgccgagttc cagggtcact
     2461 ttcttgcggc cggcgcgcgc cttcatgtcc cagccgacca gcccgccggt gaagctgagc
     2521 agggcgatgc gctcgtcctc taccagcgcc gaggcgtcgt cgttgctgca gggcatgatc
     2581 gagaacgcgc cggtgggcag gtcggtctcc gccagcacct cgccgatgat cagcgcgccc
     2641 aagggcgtct tcgccgccgg cttgagcacg aacgggcagc cggcggcgat cgccggcgcg
     2701 accttgtgcg cgaccaggtt gagcgggaaa ttgaacgggg tgatgaagct gcaggcgccg
     2761 atcgggacgc gtttggtcat gccgcggtag ccgcgcgtgc gcgccgacag ttgcagttcc
     2821 agcacctcgc caccgatgcg cgtggcctcg ccggcggcga tgcggaaggt gtcgaccagg
     2881 cgcgtgactt cgccgcgcgc atccttgatc ggcttgccgg cctcgatgca cagggccagc
     2941 gcgagttcct cgctgcgcgc ttcgaagcgc gccacgcaat gttccagcac gtcgcgtcgg
     3001 gcatccggcg ggaacgcggc catggcctcg cgcgccgcgt aggcggcggc gatggcgtcc
     3061 tcgatcgccg cggcatcggc aaaggccacg cgggccacgc gttcgccgct gaacttgtcg
     3121 agcacgtcca gcctggtatt cgcctgcacg ggctggttgg cgaggtagta gggccagcgc
     3181 ttcttcatga tccggactcc gttccttcga cccgcaggcg caggcggcca tcgtgcaggc
     3241 gcgcctgcag cgcttcgccc gggtgggtgt cgccgatgcc gcggaccacg cggccgtctt
     3301 cgcgctgcag gatgctgtaa ccgcgcgcga ccgtggccag cgggctgatc gcatgcagcg
     3361 accgcgccaa cccgcgcagg cgcagcgcct cgtgctgcag gtggcgggcg agcagcgatt
     3421 gcgggcgtcc gcgcagcgcc tgcaggcgtt cgcgcaggcg gtcgatgcgg cggcgtggat
     3481 gcgccgcccg cagcaccgca tccgcatgcc gaaggcgtgc acgttcctgc tccagccggc
     3541 gctgcaacgc attggccaat cgcaactggg cctgctcgcc gcgacgcgcc agggcgtcca
     3601 gg
//
LOCUS       DAOOCX010000201         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_24879_length_3598_cov_4.815693, whole genome shotgun sequence.
ACCESSION   DAOOCX010000201 DAOOCX010000000
VERSION     DAOOCX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24879_length_3598_cov_4.815693"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /gene="selD"
                     /locus_tag="PK399_12335"
     CDS             complement(<1..966)
                     /gene="selD"
                     /locus_tag="PK399_12335"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPW13950.1"
                     /translation="MTDIQPADRPAAPRLTSLAHGGGCGCKIAPGVLSQILKSSSGFP
                     IPKELLVGIETADDAAVYRLNNEQALIATTDFFMPIVDDPRDFGRIAATNAISDVYAM
                     GGTPILALALVGMPINVLSTQTIGRILDGGASVCRAAGIPVAGGHSIDSVEAIYGLVA
                     LGLVHPQRVKRNADAKPGDVLVLGKPLGVGIMSAALKKGQLGDAGYAQMLATTTRLNT
                     PGPDLAELPGVHAMTDVTGFGLAGHALEMARGAGCDVQIDWNAVPLLPGVRALAAQGM
                     VTGASGRNWAGYGAQAVLPNGFAAEDRALLTDPQTSGGLLVACEPA"
     gene            complement(963..1946)
                     /gene="senB"
                     /locus_tag="PK399_12340"
     CDS             complement(963..1946)
                     /gene="senB"
                     /locus_tag="PK399_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HPW13951.1"
                     /translation="MLIVSPALAAANNGNWQTASRWARLLRPDFHTRVLGDWHGEPCD
                     ALIALHARRSAHAVAAYTAAHPTRPCIVALTGTDLYRDIRDDSSAQESLRRASHLVVL
                     QEQGIAALPPAMRAKARVIFQSAPTRQPAPPPGRVLRVIFVGHLRAEKDPMTLLRAAS
                     RLACRRDIRIDLIGDGQDCALAESARLAQATQANFRWLGARPHAETRRRIQRAHLLVN
                     SSLMEGGAHVILEAVRSGTAVLASHVAGNIGMLGEAHAGYFEVGDDAALAALIETACD
                     DALFLARLRAAGESRSALFSPSIEKQRLHQLVAESLRPAVIHAQTPQETRP"
     gene            2001..3293
                     /gene="senA"
                     /locus_tag="PK399_12345"
     CDS             2001..3293
                     /gene="senA"
                     /locus_tag="PK399_12345"
                     /inference="COORDINATES: protein motif:HMM:NF041186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HPW13952.1"
                     /translation="MNKKAPEKWVSDTTDVDSPLLRCAGKDLLSLALMDSRNHTLQWL
                     SVYESGLDATDWRVPMRPELNPPLWAAGHVGWFQEHWIGRHVQRQRGVGCDPTGIRLA
                     SIEPQADDWYDSSAVPHDTRWSLDLPGLQRTKDYLAETMEITLDLLDATDVETGDPDA
                     ALYFHRLALFHEDMHGEAFAMMAQTLGLTLPDSDDRAPPLLRHASGRSQSVQRPPLTF
                     LPTRWTLGLARGNGFVFDNETDAHEVAVPEFEIDSQAVTWAQYAEFVEDGGYDDVRWW
                     SAAGWQWVQRAGRRVPRYVDQLRQGVLQRRFGVLVRLPMHQPVVHVSWYEADAWCRWA
                     GRRLPTEVEWEVAAHAGAARGFRWGDVWEWTASTFREYPGFRVDPYRDYSKPWFGTHK
                     VLRGASFATSGRMRHPKFRNFYLPERDEMFCGFRSCST"
     gene            complement(3298..>3598)
                     /locus_tag="PK399_12350"
     CDS             complement(3298..>3598)
                     /locus_tag="PK399_12350"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW13953.1"
                     /translation="VVASGSADAAFGIEVAALDNGLEFVPLARENYFLVTLAAQLGHP
                     HVAELLRALRDPAWRSIIESIPGYSAEGSGEVLSLRAVLPWWTYRKPKPGAEPPT"
BASE COUNT          562 a         1195 c         1246 g          595 t
ORIGIN      
        1 cgccggctcg caggccacca gcaagccgcc gctggtctgc gggtcggtga gcaaggcgcg
       61 gtcctcggcg gcaaagccgt tgggcagcac cgcctgcgcg ccgtagcctg cccagttgcg
      121 gcccgacgcg ccggtgacca tgccctgcgc cgccagcgcg cgcacgccgg gcagcagcgg
      181 caccgcgttc cagtcgatct gcacatcgca gcctgcgccg cgcgccatct ccagcgcatg
      241 gccggccagg ccgaagccgg tgacatcggt catcgcgtgc acgcccggca actcggccag
      301 gtccgggccg ggtgtgttga gccgcgtcgt cgtggccagc atttgcgcgt aacccgcgtc
      361 gccgagttgg cccttcttca gtgccgcgct catgatgccc acgcccagcg gtttgcccag
      421 caccagcacg tcgccgggct tcgcgtcggc attgcgcttg acgcgctgcg gatgcaccag
      481 ccccagcgcc accaggccgt agatggcctc caccgaatcg atgctgtggc cgccggccac
      541 cggaatgccc gcggcacggc acaccgacgc gccgccgtcc aagatgcggc cgatggtctg
      601 cgtggacagc acgttgatcg gcatgccgac cagcgccagc gccaggatcg gcgtgccgcc
      661 catcgcgtag acatcgctga tcgcgttggt cgccgcgatg cgtccgaagt cgcgcgggtc
      721 gtcgacgatc ggcatgaaga agtccgtcgt cgcgatcagt gcctgctcgt tgttgagccg
      781 gtaaaccgct gcatcgtctg cggtctcgat gcccaccagc agttccttgg gtatcgggaa
      841 gcccgaactg ctcttcagga tctgcgagag cacgccaggc gcgatcttgc agccgcagcc
      901 gcctccatgg gccagcgagg tcaggcgcgg ggcggcaggg cggtcggcgg gctggatgtc
      961 agtcatgggc gggtttcctg tggagtctgg gcgtggatca cggcaggtcg caatgactcg
     1021 gcgaccagtt gatgcagtcg ctgtttttcg atggatggag agaacaacgc cgagcggctt
     1081 tcccccgctg cgcgcagacg cgccagaaac agcgcgtcgt cgcaggctgt ctcgatcagc
     1141 gctgccaagg ctgcatcatc gccgacctcg aaatagcctg cgtgtgcctc ccccagcatc
     1201 ccgatgttgc cggcgacgtg cgaggccagc acggcggtcc cgcttcgcac cgcttcgagg
     1261 atcacatggg cgccgccttc catcagactg ctgttgacca gcaggtgcgc gcgctggatg
     1321 cgccggcgcg tctcggcatg cggccgtgca ccgagccagc ggaagttcgc ctgcgtcgcc
     1381 tgtgcgaggc gcgccgattc ggccagcgcg cagtcctgtc catcgccaat caggtcgatg
     1441 cggatatcgc gccggcacgc gagccgtgag gcggctcgca gcagcgtcat ggggtctttc
     1501 tcggcacgca ggtgaccgac gaatatcacg cgcaggaccc gtccgggtgg cggcgccggt
     1561 tggcgagtgg gcgccgactg gaagatcacg cgagcctttg cgcgcatggc gggcggcagg
     1621 gcagcgatgc cctgctcctg cagcaccacc aggtgagagg cgcgccgcag cgactcctgc
     1681 gcggaggagt catcgcggat gtcacggtac aggtcggttc cggtcagcgc gacgatgcag
     1741 gggcgcgtcg ggtgcgcagc cgtgtaggcc gcgacggcgt gggccgagcg ccgcgcatgc
     1801 agcgcgatca aggcgtcaca gggctcgccg tgccagtcgc caagcacccg ggtgtgaaaa
     1861 tcggggcgca acaaccgggc ccagcgtgat gcggtctgcc agttgccgtt gttggctgcg
     1921 gccagcgcag gactcacgat cagcacggtg ggtcggtgag ttctggcgat cacgatgcaa
     1981 ggcccgatgc tggagacgac ttgaacaaga aggcaccgga aaaatgggtc agtgacacga
     2041 cggacgtgga ttcgccgctg ctgcgctgcg ccggcaagga cctgctgtcg ctggcgctca
     2101 tggactcgag aaatcatacg ttgcagtggt tgtcggtcta cgagtcaggt ctcgacgcga
     2161 ctgactggcg cgtcccgatg cgccccgaac tgaatccgcc actttgggcg gcggggcacg
     2221 tcggctggtt ccaggagcac tggatcggtc gtcacgtcca acgccagcgc ggcgtcggtt
     2281 gcgaccccac cggcatccgg ctcgcttcga tcgaacccca ggccgacgac tggtacgact
     2341 cgtccgccgt gccgcacgac acccgctggt cactcgacct gcccggcctg cagcgcacca
     2401 aggattacct ggccgagacg atggagatca cgctcgacct gctcgacgcg accgatgtcg
     2461 agactggtga tccggatgcc gcgctctatt tccacaggct ggcgctgttc catgaagaca
     2521 tgcacggcga ggcgttcgcg atgatggccc agacgctggg cctgacgttg cctgacagcg
     2581 acgaccgcgc accgcctctg ctgcggcatg cgtcgggccg gagccaatcg gtgcagcggc
     2641 cgccgctgac gttcctgccg acgcgctgga ccttgggcct ggcgcgtgga aacggtttcg
     2701 ttttcgataa cgagaccgat gcccacgagg tggctgtgcc cgagttcgag atcgactcgc
     2761 aggccgtcac ctgggcacag tacgccgagt tcgtagagga cggtggctac gacgacgtgc
     2821 gatggtggtc tgcagccggc tggcagtggg tgcaacgcgc aggccggcgg gttccgaggt
     2881 acgtcgacca attgcggcag ggcgttcttc agcggcgctt cggtgtgctg gtgcgcttgc
     2941 cgatgcacca gccggtggtt cacgtcagct ggtatgaggc cgatgcctgg tgccgatggg
     3001 ccgggcgccg attgccgacc gaggtcgaat gggaagtggc tgcacacgcg ggtgccgcgc
     3061 gaggttttcg ctggggggat gtctgggagt ggaccgcaag cacctttcgc gagtaccccg
     3121 gtttccgggt cgatccctat cgcgactact cgaagccctg gttcggaacg cacaaggtat
     3181 tgcgtggggc ctcgttcgcc accagcggtc gcatgcggca cccgaaattc cgcaacttct
     3241 atctgccgga gcgcgacgag atgttctgcg gcttccgcag ttgctcgacc tgatgcctca
     3301 ggtcggtggc tccgcccctg gcttcggctt gcgataggtc caccatggca gcaccgcgcg
     3361 cagcgacagc acttcgccgc tgccttcggc gctgtagccc gggatggact cgatgatcga
     3421 gcgccatgcc ggatcgcgca gcgcacgcag cagttccgcg acatgcgggt gccccagctg
     3481 cgcggcgagt gtcaccagga agtagttctc ccgcgccagc ggcacgaact cgaggccgtt
     3541 gtcgagcgca gcgacctcga tgccaaaggc cgcatcggcg ctgccgctgg caaccacc
//
LOCUS       DAOOCX010000202         3581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_25045_length_3581_cov_6.836358, whole genome shotgun sequence.
ACCESSION   DAOOCX010000202 DAOOCX010000000
VERSION     DAOOCX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25045_length_3581_cov_6.836358"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..555
                     /locus_tag="PK399_12355"
     CDS             166..555
                     /locus_tag="PK399_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPW13954.1"
                     /translation="MPADHPCLSCGACCAFFRVSFHWSEADPALGGQVPFELTEPLRT
                     HERVMRGTSQKDPRCIALDADIGRYSRCTIHDRRPSVCALVPASWEFGAASAQCDKSR
                     IAHGLPALTPADWIGVDEAEKNPLPEP"
     gene            complement(561..1244)
                     /locus_tag="PK399_12360"
     CDS             complement(561..1244)
                     /locus_tag="PK399_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPW13955.1"
                     /translation="MDFDALDRSLRESAQDFTLDADEKVQLRELGAQLDAPRVRFLRN
                     RAFDIAREAMLATPADTLDVLRWLEQVVKTLDAANEAAPVVSSAYFSPGDTCLRRLRD
                     LMRSCKATLDICVFTIADDRLTEAILECHARGVQVRVVSDDDKQHDSGSDIARLRAGG
                     VAVRLDDSPYHMHHKFALVDGRLLANGSFNWTRSATEKNDENLVVTDDANLVRVFGLQ
                     FEKLWQQFD"
     gene            complement(1244..2158)
                     /locus_tag="PK399_12365"
     CDS             complement(1244..2158)
                     /locus_tag="PK399_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016849672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPW13956.1"
                     /translation="MQFPDYPFAPQRFEVRPGIAMSYLDEGPRDGEVIVMLHGNPSWS
                     FYWRHLVNGLKDRYRCIVPDHVGMGLSDRPDDAASASPRYDYTLQSRIDDLDALLRHL
                     GIDGPLTLAVHDWGGMIGFGWALRDPARVRRLVITNTASFPLPASKRFPKRLAMGRDS
                     RPGGWLIRRFNLFARGAAWFGTSRSLPKDVRAAYAGVYDGWDQAISTLRFMQDIPLDP
                     GDRAMPIVQAAAAALPSYADRPAFIGWGLQDFVFDQHFLAGFRKALPRALVSEFDDAG
                     HYVLEDKHEVLVPAIRRFLDAHPLVGTA"
     gene            2271..3185
                     /locus_tag="PK399_12370"
     CDS             2271..3185
                     /locus_tag="PK399_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPW13957.1"
                     /translation="MAFDAFALILTMLGIGMLFARLRVLPDNAAEVLNRIVLVVCLPA
                     AVLTYVPRLHMQASLLGIVATPWLLAAATLGLVTLASHWLRFRRDEHAVLLLCVALGN
                     SSFIGYPMVQALLGEHALPYAVVYDQFGIFVLLSTFGLYVLARYGGDAPPTLRTVAVR
                     MLRFPPLWALLLGLTLMPATPPAWIAGGLQRLADALLPLVMLAVGLTIQLRLPRDEVK
                     PLAVGLALRLLVLPALALPLSLAFGLRDDMLRANVLESAMPSMITAAALAISHQLAPR
                     LAAALVGYGILLSLLTLPAWAWLLQRFG"
     gene            complement(3196..>3581)
                     /locus_tag="PK399_12375"
     CDS             complement(3196..>3581)
                     /locus_tag="PK399_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HPW13958.1"
                     /translation="ATEWNKLCRGNLDGMVTDTRMLLIEGIKLAQKTFVAAKHALGWV
                     SEELDQFVIHQVSKVHTAAFTKAIGIDPRKVLTIFGEHGNIGPASVPIVLSKLREMGR
                     LKKGDRVALLGIGSGLNCSMAEVVW"
BASE COUNT          510 a         1247 c         1227 g          597 t
ORIGIN      
        1 cgagttgcgc gtaggtcagc gcgacctcgt agcggccgtt gcggccgggg cagcgtatcg
       61 ccacctggtc cgggcgctcg cgggcgagcc ctggcagcgc ggccgcgatg ttgcaggtct
      121 cgggtggcat ctcgctcatg cgcgcattgt cgccgacaat ggcgcatgcc cgccgaccat
      181 ccctgcctca gttgcggtgc ctgctgcgcg ttcttccggg tcagtttcca ctggtcggag
      241 gccgatcccg cgctgggcgg ccaggtcccc ttcgagttga cggagccgct gcgcacgcac
      301 gagcgggtga tgcgcggcac ctcgcagaaa gacccgcgct gcatcgcgct ggatgccgac
      361 atcggccgct attcgcgctg cacgatccac gaccgccggc cgtcggtgtg cgcactggtg
      421 ccggcaagct gggagttcgg cgcggccagc gcgcaatgcg acaagtcgcg gatcgcgcac
      481 ggcctgcctg cgttgacgcc ggccgactgg atcggcgtgg acgaggccga gaagaatccg
      541 ctacccgaac cctgacagcc tcagtcgaac tgctgccaga gtttttcgaa ctgcaggccg
      601 aacacgcgca ccaggttggc gtcgtcggtc accaccaggt tctcgtcgtt cttctcggtc
      661 gcgctgcggg tccagttgaa gctgccattg gccagcaggc gcccgtcgac cagcgcgaac
      721 ttgtggtgca tgtggtacgg gctgtcgtcc agccgcaccg ccacgccgcc cgcgcgcagg
      781 cgggcgatgt cgctgccgct gtcgtgctgc ttgtcgtcgt cgctgaccac ccggacctgc
      841 acgccgcgcg cgtggcattc gaggatcgcc tcggtgaggc gatcgtcggc gatggtgaac
      901 acgcagatgt ccagcgtggc cttgcagctt cgcatgaggt cgcggagccg gcgcaggcag
      961 gtgtcgcccg gcgagaagta ggcgctggac acgactggcg cggcctcgtt cgccgcgtcc
     1021 agcgtcttca ccacctgctc gagccagcgc agcacgtcca gcgtgtccgc aggcgtggcc
     1081 agcatggctt cgcgggcgat gtcgaacgcg cggttgcgca ggaagcgcac gcgcggcgcg
     1141 tccagttgcg cgccgagctc gcgcagttgc accttctcgt ccgcatccag ggtgaagtcc
     1201 tgcgcggact cgcgcaggct gcggtccagc gcgtcgaaat ccatcacgcg gtaccgacca
     1261 gcggatgcgc gtccaggaag cgacggatcg ccggcaccag cacctcgtgc ttgtcctcca
     1321 gcacgtaatg cccggcatcg tcgaattcgc tcaccagggc gcgcggcagc gccttgcgga
     1381 agccggccag gaaatgctgg tcgaacacga agtcctgcag gccccagccg atgaaggcgg
     1441 gacgatccgc gtagcttggc aacgccgcgg cggcggcctg cacgatcggc atcgcgcggt
     1501 cgccgggatc cagcgggatg tcctgcatga agcgcagggt gctgatcgcc tggtcccaac
     1561 cgtcgtacac cccggcatag gcggcgcgca cgtccttcgg caaggaccgg ctggtaccga
     1621 accaggccgc gccgcgtgcg aacaggttga agcggcggat cagccagccg cccggcctcg
     1681 aatcgcggcc catcgccagt cgcttcggga agcgcttgct cgcgggcagc gggaacgacg
     1741 cagtattggt gatcaccagc cgcctcaccc gcgccggatc gcgcagcgcc cagccgaagc
     1801 cgatcatccc gccccagtcg tgcaccgcca gggtcagcgg gccatcgatg ccgagatgcc
     1861 gcagcagggc atccaggtcg tcgatccgcg actgcagggt gtagtcgtag cgcggcgacg
     1921 cggacgcggc atcgtccggc cgatccgaca ggcccatgcc cacgtggtcg ggcacgatgc
     1981 agcggtagcg atccttcagt ccgttgacca ggtgccgcca gtagaagctc catgacggat
     2041 tgccgtggag catgacgatc acctcgccat cgcgcgggcc ttcgtccagg tacgacatgg
     2101 cgatgccggg tcgcacctcg aagcgttgcg gggcgaaggg gtagtcgggg aactgcatgc
     2161 gcggccggat cctgcgcggc gcgggccgca tggccgcaaa ggataccggc gcaggccact
     2221 gcgaccaatt cccgatggcg agccacggtc cacgcggcga gaatccccgc atggccttcg
     2281 acgcattcgc cctgatcctg accatgctcg gcatcgggat gctgttcgcg cggctgcgcg
     2341 tgctgccgga caacgccgcc gaagtcctca accgcatcgt cctggtcgtc tgcctgccgg
     2401 cggcggtgct gacctacgtg ccacggttgc acatgcaggc ctccctgctg ggcatcgtgg
     2461 ctaccccgtg gctgctggcc gcggccaccc tcggcctggt gaccctggcc agccactggc
     2521 tgcgcttccg ccgcgacgaa cacgccgtgc tgctgctgtg cgtggcgctg ggcaacagca
     2581 gcttcatcgg ctacccgatg gtgcaggcgc tgctgggcga acacgccctg ccgtatgccg
     2641 tggtctacga ccagttcggc atcttcgtgc tgctgtccac cttcggcctg tacgtgctcg
     2701 cgcgctacgg cggcgatgcg ccgccgacct tgcgcacggt cgcggtgcgg atgctgcgct
     2761 tcccgccgct gtgggcgctg ctgctgggcc tgacgttgat gccggccacg ccccccgcgt
     2821 ggatcgccgg cggcctgcag cggctggccg atgccctgct gccgctggtg atgctggcgg
     2881 tgggcctgac catccagctg aggctgccgc gcgacgaggt caagccgctc gcggtgggcc
     2941 tggccttgcg cctgctggta ctgccggcgc tggccctgcc actgtcactg gcgttcggcc
     3001 tgcgcgacga catgctgcgg gcgaacgtgc tggaatccgc gatgcccagc atgatcaccg
     3061 ccgccgcgct ggcgatctcc caccagctgg cgccgcggtt ggcggccgcg ctggtgggtt
     3121 acgggatcct gttgtcgctg ctgaccctgc cggcgtgggc gtggctgctg caacgcttcg
     3181 gttgaagccg tccggttacc agaccacttc cgccatcgag caattcaagc ccgagccgat
     3241 ccccagcaag gccacccggt cgcccttctt caggcgcccc atctcgcgca gcttggacag
     3301 cacgatcggc accgatgccg gaccgatgtt gccatgttcg ccgaagatgg tcaggacctt
     3361 gcgcggatcg atgccgatcg ccttggtgaa ggcggcggta tgcaccttgc tgacctggtg
     3421 gatgacgaac tggtccagtt cctcggacac ccagcccagc gcgtgcttgg cggcgacgaa
     3481 ggtcttctgc gccagcttga tgccttcgat cagcagcatg cgggtgtcgg tgaccatgcc
     3541 gtccaggttg ccgcggcaca gcttgttcca ctcggtcgcg g
//
LOCUS       DAOOCX010000203         3525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_25609_length_3525_cov_6.738040, whole genome shotgun sequence.
ACCESSION   DAOOCX010000203 DAOOCX010000000
VERSION     DAOOCX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25609_length_3525_cov_6.738040"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PK399_12380"
     CDS             complement(<1..939)
                     /locus_tag="PK399_12380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW13959.1"
                     /translation="MLRPFPHSGCPMRPALLAIALSIAVATPALAQDAAKPKYRSAKD
                     IIDAAPASAWRDLDPANTLYMELPAGRVVIELAPGFAPEHVANIKTMARGGFWDGLAI
                     YRSQDNFVVQFGDAEGEEAGKAKPYPAGTKTHLPAEFTRDASGARFDKLPDRDGYAPE
                     VGFIDGFPAARDPKAGKAWMAHCYGMLGAGRNNAPDSSIGAELYVVTGQSPRALDRQL
                     SAVGRVVKGMEFLSVIPRGPEPMGFYEKPEQRAPIVSIKLASDVPEAQRTPLQLLRTD
                     SQAFIDATEARRNRVDGFYTIPVGHIDLCNVPLPVRA"
     gene            1066..1425
                     /locus_tag="PK399_12385"
     CDS             1066..1425
                     /locus_tag="PK399_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005911694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPW13960.1"
                     /translation="MDEALLRKFHKELSAGTVSLALLAVVAASTEPMYGYQIAKRLEQ
                     LGDGVLAGKQSALYPVLRNLEGAGLLDSYVEPSVSGPPRRYYRATAAGRDALTDWSAA
                     WRATRDSLDAVLKEASA"
     gene            1422..2333
                     /locus_tag="PK399_12390"
     CDS             1422..2333
                     /locus_tag="PK399_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HPW13961.1"
                     /translation="MNAVHDLPTTIPAYLERLRQALQGCDPALAQDALYDAEDYLRSE
                     LAANPGKSEAEVIAAVAGSYGAPDEVADIYRDTEVRVQTALRAPPAPPRRSLPGRFFG
                     VLADPHTYGSLFYMLLSLATGTFYFTWAVTGLSLSLGLAILVIGIPFAILFIGATRVL
                     ALVEGRIVEVMLGERMPRRPVYSDRSLPWLQRIGAMFTDPRTWGTMAYFLAMQPLGVV
                     YFAVAVTTLALGAAFIAMPFAHWFGFDANIHLDGHGWIDWSAPWAWPLSIVIGIVLII
                     AMLHLARLVGHLHGKLAKALLVSSQAY"
     gene            2336..3274
                     /locus_tag="PK399_12395"
     CDS             2336..3274
                     /locus_tag="PK399_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13962.1"
                     /translation="MRRLKRVALALLVSLVVSLGGGYVYFDRKFTPPENALSVSSMAT
                     EVPIRWLATNGNPHAALLLPVKLDGIADTFYMQLDLGSPSTVFYGNAIRSVLEQRRAG
                     FAIGHGARQLGLGFSIDGLRIRSGSFGLLDYGSKVEFGNPAAANIIGTIGTDLLEKRI
                     LVLDFKRGTTAFLQEVPEAGFSGFKFTKRRVLIPARIAGRDGMFLYDSGSSGYALLTS
                     KQEWDRLRTPGGAIKVDTGNSWGRALKVMTAPAAQTLALSNATLRLSEVTYVEGTSRM
                     QEWLMRSSGMQGMLGNKLFLGHTLTLDAKRQRFKLD"
     gene            complement(3326..>3525)
                     /locus_tag="PK399_12400"
     CDS             complement(3326..>3525)
                     /locus_tag="PK399_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HPW13963.1"
                     /translation="AHKLCTGIPAADRKHVTVEGAGHYGIFSGRRWRTQVYPQVRDFI
                     AAHAGGVESTPGKTPKKRPTR"
BASE COUNT          525 a         1160 c         1234 g          606 t
ORIGIN      
        1 cgcccgcacc ggcagcggca cgttgcacag gtcgatgtgg ccgaccggga tcgtgtagaa
       61 gccgtcgacg cggttgcggc gggcctcggt ggcgtcgatg aaggcctggc tgtcggtgcg
      121 cagcagttgc agcggggtgc gctgcgcctc gggcacgtcg ctggccagct tgatcgagac
      181 gatcggcgcg cgctgctcgg gcttctcgta gaagcccatc ggttccgggc cgcgcgggat
      241 cacgctcagg aattccatgc ccttgaccac ccggccgact gcgctcaact ggcgatcgag
      301 cgcgcgcggc gactggccgg tgaccacgta gagttcagcg ccgatgctgc tgtccggggc
      361 gttgttgcgg ccggcgccga gcatgccgta gcagtgcgcc atccacgcct tgccggcctt
      421 cgggtcgcgg gcggcgggaa agccgtcgat gaagccgacc tccggcgcat agccgtcgcg
      481 gtcggggagt ttgtcgaagc gcgcgccgct ggcatcgcgg gtgaattcgg cgggcaggtg
      541 ggtcttcgtg ccggccgggt agggcttggc cttgccggct tcctcgccct cggcatcgcc
      601 gaactggacc acgaagttgt cctgcgagcg atagatcgcc agcccgtccc agaagccgcc
      661 cctggccatg gtcttgatgt tggccacgtg ttccggggcg aagccgggcg ccagttcgat
      721 caccacgcgg ccggccggca actccatgta cagggtgttg gccgggtcga ggtcgcgcca
      781 ggcgctggcc ggcgcggcgt cgatgatgtc cttggcgctg cggtacttcg gcttggcggc
      841 gtcctgggcc agggcgggcg tggcgacggc aatcgacagg gcgatggcga gcaaggcagg
      901 gcgcatgggg catccggaat gcgggaaagg ccgaagcata gcgtccgcgc cgggccgggc
      961 ggggtggcct gggtcgggct aatgtgagcg catgactact ggcacattcg ctatatcgaa
     1021 atgaacaata gtattcgcta caacatagac ccggcggatc cgcagatgga cgaagccctc
     1081 ttacggaaat tccacaagga gctcagcgcc ggcacggtct cgctggccct gctggcggtg
     1141 gtggcggcct ccaccgagcc gatgtacggc taccagatcg ccaagcggct ggagcagctc
     1201 ggcgatggcg tgctggccgg caagcagagc gcgctgtatc cggtgctgcg caacctggaa
     1261 ggggccggcc tgctggacag ctacgtcgag ccctcggtca gcggcccgcc gcgccgttat
     1321 taccgcgcca ccgccgccgg gcgcgacgcc ctcaccgact ggtccgccgc ctggcgcgcc
     1381 acccgcgatt ccctcgatgc cgtgctcaag gaagccagcg catgaacgcc gtccacgacc
     1441 tgccgaccac catccccgcc tacctggaac gcctgcgcca ggcgttgcag ggctgcgatc
     1501 ccgcgctggc ccaggatgcg ctgtacgacg ccgaggacta cctgcgctcg gaactggccg
     1561 ccaatcccgg caagtccgag gccgaggtga tcgccgcggt cgccggcagc tacggtgcgc
     1621 ccgacgaggt cgccgacatc taccgagata ccgaggtgcg cgtgcagacc gcgttgcgcg
     1681 cgccgccagc gccgccgcgc aggtcgctgc ccggccgctt cttcggcgtg ctggcggatc
     1741 cgcataccta cggctcgctg ttctacatgt tgctgtcgct ggccaccggc acgttctact
     1801 tcacctgggc ggtgaccggg ctctcgctct cgctggggct ggcgatcctg gtcatcggca
     1861 ttccgttcgc gatcctgttc atcggcgcca cccgcgtgct ggccctggtg gaagggcgca
     1921 tcgtcgaggt gatgttgggc gaacgcatgc cgcgccgccc ggtctactcg gatcgcagcc
     1981 tgccctggct gcagcggatc ggcgcgatgt tcaccgaccc gcgcacctgg ggaacgatgg
     2041 cctacttcct ggcgatgcag ccgctgggcg tcgtctactt cgcggtcgca gtgaccacgc
     2101 tggcgctggg tgccgccttc atcgcgatgc cgttcgcgca ctggttcggc ttcgacgcca
     2161 acatccacct ggacgggcat ggctggatcg actggtcggc gccgtgggcc tggccgttga
     2221 gcatcgtgat cggcatcgtc ctgatcatcg cgatgctgca cctggcgcgg ctggtgggcc
     2281 acctgcacgg caagctggcg aaggcgctgc tggtcagcag ccaggcgtat tgagcatgag
     2341 gaggctgaaa cgcgtcgcgc tggcgttgct tgtctcgctg gtggtgtcgt tgggtggcgg
     2401 gtacgtgtat ttcgacagga agttcacccc gccggaaaat gccctgtccg tgtcgagcat
     2461 ggcgacggag gtcccgatcc ggtggcttgc gacgaacggc aacccgcatg ccgcgctcct
     2521 cctgccggtg aagctcgatg gcatcgcgga cacgttctac atgcaactgg acctgggttc
     2581 gccgagcacg gtcttctacg gcaatgccat ccgctccgtg ctcgagcagc gccgtgcggg
     2641 cttcgcaatc gggcacggtg cccgccagct tggcctgggc ttctcgatcg acggactgcg
     2701 cattcgctcc gggagcttcg ggttgctcga ctacggcagc aaggtcgagt tcggcaaccc
     2761 ggcagcggcc aacatcatcg gcacgatcgg gaccgacctg ctggagaagc gcatcctggt
     2821 cctggatttc aagcggggaa ccaccgcttt cctgcaggag gttccggagg cggggttctc
     2881 cgggttcaag ttcaccaagc gcagggtgct gatccccgca cgcatcgcgg gccgggacgg
     2941 catgttcctg tacgactcgg gatccagcgg ctatgcattg ctcaccagca agcaggaatg
     3001 ggacaggctg cggacgcccg gtggggcaat caaggtcgac accggcaatt cgtgggggcg
     3061 cgcgttgaag gtgatgacgg cacccgcggc gcagacactt gcgctttcga acgccacgct
     3121 gcgactgtcc gaagtgacct acgtcgaagg gacatcgcgg atgcaggaat ggctgatgcg
     3181 cagctccggc atgcagggga tgctcggcaa caagctgttc ctgggccaca cgctcacgct
     3241 tgacgcgaaa cgccagagat tcaagctcga ctgacggccg tcgaggcgag cggcatcgaa
     3301 cgcccatgcc gcggcatgca tcgcctcagc gcgtgggcct cttcttcggc gtcttccccg
     3361 gcgtgctctc cacgcctcct gcatgcgcgg cgatgaagtc gcgtacctgc ggatacacct
     3421 gcgtgcgcca gcgacggccg ctgaagatgc cgtagtggcc ggcgccttcc acggtcacgt
     3481 gcttgcggtc ggccgcgggg atgccggtgc acagcttgtg cgcgg
//
LOCUS       DAOOCX010000204         3520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_25658_length_3520_cov_8.854834, whole genome shotgun sequence.
ACCESSION   DAOOCX010000204 DAOOCX010000000
VERSION     DAOOCX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25658_length_3520_cov_8.854834"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..86)
                     /locus_tag="PK399_12405"
     tRNA            complement(1..86)
                     /locus_tag="PK399_12405"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Tyr,seq:gta)
     gene            complement(176..1267)
                     /gene="ychF"
                     /locus_tag="PK399_12410"
     CDS             complement(176..1267)
                     /gene="ychF"
                     /locus_tag="PK399_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPW13964.1"
                     /translation="MGIKCGIVGLPNVGKSTLFNALTKAGIAAANFPFCTIEPNVGIV
                     PVPDPRLNALSEIVKPQKCIPTAVEFVDIAGLVAGAASGEGLGNKFLAHIREVDAITH
                     VVRCFENDDVIHVNNKVDPLADIDTIDTELALADLESVDKALNKAERSAKTGDKDAKA
                     RAEVLGRISAGLNEGRSARSLGLSDDDKAAVRDLFLITLKPVMYVANVLEDGFENNPH
                     LDAVRARAAGEGAEVVPVSAAIEEELSQLDDADRDEFLKDLGLDEPGLNRVIRAAYKL
                     LGLQTYFTAGVKEVRAWTVKAGSTAPQAAGVIHTDFEKGFIRAETIGYDDFLKYRGEA
                     GARDAGRLRLEGKEYRVQEGDVLHFRFNV"
     gene            complement(1326..1910)
                     /gene="pth"
                     /locus_tag="PK399_12415"
     CDS             complement(1326..1910)
                     /gene="pth"
                     /locus_tag="PK399_12415"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007968854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPW13965.1"
                     /translation="MAGLRLIVGLGNPGPEHARTRHNAGFWFIDALAEQAGARFGLDS
                     KLFGEIAKAGIGGHDVWLLKPATFMNLSGKSITAALRYWKIEPEEMLVAHDELDMPPG
                     VARLKFDGGHGGQNGLRDTMRLLGHGKFHRLRVGIGHPGHKDKVTPWVLGKPGRDDEA
                     SILRAIDDAIGVLPLAMDGNYQDAMTRLHTPRPS"
     gene            complement(1976..2626)
                     /locus_tag="PK399_12420"
     CDS             complement(1976..2626)
                     /locus_tag="PK399_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPW13966.1"
                     /translation="MATHEINVERRAVEGKGASRRLRTGGKIPAIVYGGGIDPVAIQL
                     DHEKVWLAQQNEWFYSSILDLSLGGDVQKVLLRDIQRHPFKQLIMHLDFQRVSADQAL
                     RAKVPLHFLNEDKSPAGKAADVVVLHELNDVEVSCLPKDLPEFIEIDLSNLAVGDIIH
                     LSELKLPAGVEIPELKLGKEHDVAVVIAKHGKEDTEEAAPEAADVPATKAAKKDDK"
     gene            complement(2736..>3520)
                     /locus_tag="PK399_12425"
     CDS             complement(2736..>3520)
                     /locus_tag="PK399_12425"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPW13967.1"
                     /translation="QPTNAPTAENFMELLVLVDALKRASAASVTAVVPYFGYARQDRR
                     PRSSRVPITAKVAAKMFGAVGTDRVLTVDLHADQIQGFFDMPVDNVYASPLLLADIWR
                     AHGTDNLIVVSPDVGGVVRARAIAKRLDDADLAIIDKRRPKANVSTVMNIIGDVEGKT
                     CVLVDDIVDTAGTLCAAANALKERGALKVAAYCTHPVLSGAAIDNVTRSQLDELVVTD
                     TIRLSEAAKACPKIRQLSVAELLAETIRRVAFGESVSSLYVD"
BASE COUNT          523 a         1175 c         1182 g          640 t
ORIGIN      
        1 tggtggaggg aggtggattc gaaccaccga aggcgtaagc cagcagattt acagtctgcc
       61 cccgttggcc gcttgggtat ccctccgatt gtcccgatgg cgaaccgaag ctccccaaag
      121 agcccgcaat tttgacgcca gctttcggga ctgtcaacgc ctatgtgcag atcggtcaga
      181 cattgaagcg gaagtgcagc acgtcgcctt cctgcacacg gtattccttg ccttccaggc
      241 gcagccggcc ggcgtcgcgg gcaccggcct cgcccctgta cttgaggaaa tcgtcgtagc
      301 cgatggtctc ggcgcggatg aagcccttct cgaagtcggt atggatcacg cccgccgcct
      361 gcggcgcggt ggatccggcc ttgaccgtcc acgcgcgaac ctccttgacg ccggcggtga
      421 agtaggtctg caggcccagc agcttgtagg cggcgcggat gacgcgattc aggccgggct
      481 cgtccaggcc gaggtccttc aggaattcgt cgcgatcggc atcgtccagc tggctcagct
      541 cttcctcgat ggcggcggag accggcacca cctcggcacc ctcgcccgcc gcccgagcgc
      601 gcaccgcgtc gaggtgcggg ttgttctcga acccgtcctc gagcacgttg gccacgtaca
      661 tcaccggctt cagggtgatc aggaacaggt cgcgcaccgc cgccttgtcg tcgtctgaca
      721 ggcccagcga ccgcgccgac ctgccctcgt tcaagccggc gctgatgcgg cccagtacct
      781 cggcgcgcgc cttcgcgtcc ttgtcgccgg tcttggcgga acgctccgcc ttgttcagcg
      841 ccttgtcgac ggactccagg tcggccagcg ccagctcggt gtcgatggta tcgatgtcgg
      901 ccagcgggtc gaccttgttg ttgacgtgga tgacgtcgtc gttctcgaag cagcgcacca
      961 catgggtgat cgcatcgacc tcgcggatat gggccaggaa cttgttgccc aggccctcgc
     1021 cgctcgccgc gcccgccacc aggccggcga tgtcgacgaa ctcgaccgca gtcgggatgc
     1081 acttctgcgg cttgacgatt tcgctcagcg cattgaggcg cggatccggc accgggacga
     1141 tgccgacgtt cggctcgatg gtgcagaacg ggaagttggc ggcggcgatg ccggccttgg
     1201 tcagcgcgtt gaaaagcgtg gacttgccga cgttcggcag gccgacgatg ccgcatttga
     1261 tgcccatggc gcgtgttgct ccgttgtcgc gcggcgtctg gccgcgcgaa gaaaaatcag
     1321 gtggatcagc ttggccgcgg cgtatgcagc cgggtcatcg cgtcctggta attgccgtcc
     1381 atcgccagcg gcagcacgcc gatggcgtcg tcgatggcac gcaggatgct cgcctcgtcg
     1441 tcgcgcccgg gcttgcccag cacccagggc gtgaccttgt ccttgtgccc gggatggccg
     1501 atgccgaccc gcaggcggtg gaacttgcca tgcccgagca ggcgcatggt gtcgcgcagg
     1561 ccgttctggc cgccatggcc gccgtcgaac ttgagccgtg caacgccggg cggcatgtcc
     1621 agctcgtcgt gcgcgaccag catttcctcg ggctcgatct tccagtagcg caacgccgcc
     1681 gtgatcgact tgccggagag attcatgaag gtcgcaggct tcagcaacca gacgtcgtga
     1741 ccgccgatgc cggccttggc gatctcgccg aacagcttgc tgtccagccc gaaccgcgcg
     1801 cccgcctgtt ccgccagcgc gtcaataaac cagaacccgg cgttgtgccg ggtccgggcg
     1861 tgttcgggac cgggattgcc cagcccgacg atgaggcgca ggcctgccat cggaagcgac
     1921 atccttcgga tccaggtcgg cgcgacacgg ggtcgcgccg agacgaatca aaggattact
     1981 tgtcgtcctt cttcgcggcc ttggtcgccg gcacgtcggc ggcttccggg gcggcttcct
     2041 cggtgtcttc cttgccgtgc ttggcgatca cgacggcgac gtcgtgttcc ttgcccagct
     2101 tcagctccgg gatctccacg ccggccggca gcttcagctc cgacaggtgg atgatgtcgc
     2161 cgacggccag gttcgacaga tcgatctcga tgaactccgg caggtccttc ggcaggcagc
     2221 tcacttccac gtcgttcagc tcgtgcagga cgaccacgtc ggccgccttg cccgccgggg
     2281 acttgtcctc gttgaggaag tgcagcggca ccttcgcgcg cagggcctgg tcggcgctga
     2341 cgcgctggaa gtccaggtgc atgatcagct gcttgaaggg gtggcgctgg atgtcgcgga
     2401 gcaggacctt ctggacgtcg ccgccgaggc tcaggtccag gatcgacgag tagaaccact
     2461 cgttctgctg ggccagccag accttctcgt ggtcgagctg gatggcgacc ggatcgatgc
     2521 cgccgccgta gacgatggcc gggatcttgc cgccggtgcg gaggcggcgg ctcgcaccct
     2581 tgccttccac tgcgcggcgt tcgacgttga tttcatgcgt tgccatgttg tttcactctc
     2641 gttgcattga agccgcctcg cggcggcgat gtcactcacc cgcgaccagg tgagtggggt
     2701 gcgacgcggc gatgccgcat cgcgggttcc tggcgtcagt ccacgtagag cgagctgacg
     2761 gactcgccga acgccacccg gcggatggtt tcggccagca gctcggccac gctgagctgg
     2821 cggatcttgg ggcaggcctt ggcggcctcg gacaggcgga tggtgtcggt caccaccagt
     2881 tcgtccagct gcgagcgggt cacgttgtcg atcgccgcgc cggacagcac cgggtgggtg
     2941 cagtaggccg cgaccttcag cgcgccgcgc tccttcagcg cgttcgcggc ggcgcacagg
     3001 gtcccggcgg tgtcgacgat gtcgtcgacc agcacgcagg tcttgccctc cacgtcgccg
     3061 atgatgttca tcaccgtgga cacgttggcc ttcggccggc gcttgtcgat gatggccagg
     3121 tcggcatcgt cgaggcgctt ggcgatcgcg cgcgcgcgca ccacgccacc cacgtcgggc
     3181 gagaccacga tcaggttgtc ggtgccatgc gcgcgccaga tgtcggccag cagcagcggc
     3241 gacgcgtaca cgttgtccac cggcatgtcg aagaagccct ggatctggtc cgcatgcagg
     3301 tccacggtca gcacccggtc ggtgcccacc gcgccgaaca tcttcgccgc gaccttggcc
     3361 gtgatcggca cgcgcgagga gcgcgggcgg cgatcctggc gggcgtaacc gaagtacggc
     3421 accaccgcgg tcacgctggc cgccgacgcg cgcttcagcg catcgaccag caccagcaac
     3481 tccatgaagt tctcggcggt cggggcgttg gtcggctgga
//
LOCUS       DAOOCX010000205         3490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_25986_length_3490_cov_6.341485, whole genome shotgun sequence.
ACCESSION   DAOOCX010000205 DAOOCX010000000
VERSION     DAOOCX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25986_length_3490_cov_6.341485"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /gene="ribB"
                     /locus_tag="PK399_12430"
     CDS             <1..1101
                     /gene="ribB"
                     /locus_tag="PK399_12430"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HPW13968.1"
                     /translation="IPELLEELRAGRMVVIVDDEDRENEGDLIMAAELVRPADINFMV
                     THARGLVCLSLTRERCAQLGLAPMVQANTAQFHTNFTVSIEAAEGVTTGISAYDRAHT
                     IRTAVRPDAKPSDLSQPGHIFPLTAQAGGVLTRAGHTEAASDLPMLAGLEPAGVLVEV
                     MNADGGMARRPELEVFAREHGLKMGSIADLIAYRLATEHTVERIDDRAIDTEFGPFRL
                     VTYRDRIAHELHFALVRGTPDKAMPTLVRVQVENPLSDLLHWRRDDFGVAATDALRAI
                     AAEGQGVMVVLSEPRNAEALLARLREQAAPAANSKDVGQWRRNGAGAQILADLGLGKL
                     RVLGTPRRQVGLAGYGLEVVETIAATAPAAAR"
     gene            1133..1600
                     /gene="ribH"
                     /locus_tag="PK399_12435"
     CDS             1133..1600
                     /gene="ribH"
                     /locus_tag="PK399_12435"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011409394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPW13969.1"
                     /translation="MPYIEGDLRAPQGARFAIIASRWNPRIVDALVDAARATFIGNGV
                     DEAAVDVVRVPGAWELPMAARDIALAGRHAAIVALGCVVRGDTRHYEQVADGASDGLM
                     RVALDTGVPVLNGVLAVDRFEDAAARAGGSHGNKGEEAALVAIEMANLREKLA"
     gene            1597..2058
                     /gene="nusB"
                     /locus_tag="PK399_12440"
     CDS             1597..2058
                     /gene="nusB"
                     /locus_tag="PK399_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPW13970.1"
                     /translation="MNRHRNDGIDPVARSRSRRRALQAIYAWQLSATSEGALIAEFAH
                     EQAHEIADLEYFEDLVRGVLRHVAQLDQALAGFIDRKVDEVDPIERAALRIAAYELLH
                     RPDVPYRVVINEAIDSTKRFGSEHGHTYVNGVLDKAALALRGSESQAARPR"
     gene            2073..3029
                     /gene="thiL"
                     /locus_tag="PK399_12445"
     CDS             2073..3029
                     /gene="thiL"
                     /locus_tag="PK399_12445"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPW13971.1"
                     /translation="MDSTVAEFDLIERIRRRVAARPDVLLGIGDDAALLQPPVGMQLA
                     VAMDTLNSGVHFPPETAPADIGWKALAVNLSDLAAMGASPAWCTLSLSLPDASTGFVE
                     GFLDGFLALAAHHDVALVGGDTTRGPLSVCVAVHGFIEPGQALRRDGACVGDDLWVSG
                     TLGDAAAALSQWQAGGAIDPALRLRLDRPTPRVSLGRALRGIAASCIDVSDGLLADLG
                     HVCKASGVGAWLDVDGLPASPVLRASFDGEARRRLQATGGDDYELCFSAPASMREAVL
                     EAASAHVPVTRIGRVVAGTGVSAVDRHGMAWARSSEGFRHFE"
     gene            3139..3264
                     /locus_tag="PK399_12450"
     CDS             3139..3264
                     /locus_tag="PK399_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13972.1"
                     /translation="MGRKFLVGMALVAILGAVGASGYQFGKFLKERERAEAARPV"
     gene            complement(3261..>3490)
                     /locus_tag="PK399_12455"
     CDS             complement(3261..>3490)
                     /locus_tag="PK399_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13973.1"
                     /translation="APKALRMNAPLLPVNAAVSAATPLETQSALAGDNKVTVVPVALI
                     EPLVKALPDPKDEAKDVEGRDPVQEPGRRGR"
BASE COUNT          513 a         1207 c         1253 g          517 t
ORIGIN      
        1 atccccgaac tgctggaaga actccgagcc ggccgcatgg tcgtcatcgt cgacgacgag
       61 gaccgcgaga acgaaggtga cctgatcatg gccgccgagc tggtgcggcc ggccgacatc
      121 aacttcatgg tcacccatgc gcgtggcctg gtctgcctat cgctgacccg cgaacgctgc
      181 gcgcaactcg ggctggcgcc gatggtccag gccaataccg cgcagttcca caccaatttc
      241 accgtcagca tcgaggcggc cgaaggcgtt accaccggca tcagcgccta cgaccgtgcg
      301 catacgatcc gcaccgcggt gcggccggat gcgaagccga gcgacctcag ccagcccggc
      361 cacatcttcc cgctcactgc acaggccggc ggcgtgctga cccgcgctgg ccataccgaa
      421 gccgccagcg acctgccgat gctggcgggg ctggaaccgg cgggcgtctt ggtcgaagtg
      481 atgaatgccg atggcggcat ggcgcgcagg cccgagctgg aagtgttcgc ccgcgagcac
      541 ggcctgaaga tgggctcgat cgccgacctg atcgcgtatc ggctcgccac cgagcacacc
      601 gtcgagcgca tcgacgatcg cgccatcgac accgaattcg gcccgttccg gttggtcacc
      661 taccgcgacc gcatcgccca cgagctgcac ttcgcgctgg tgcgcggcac gcccgacaag
      721 gccatgccga ccctggtgcg ggtccaggtc gaaaacccgc tgagcgacct gctgcactgg
      781 cgccgcgacg atttcggggt ggccgccacc gatgccctgc gcgcgatcgc cgccgaaggg
      841 cagggcgtga tggtggtgct gtccgagccg cgcaatgccg aggccttgct ggcgcgcctg
      901 cgcgagcagg ccgcgccggc cgcgaacagc aaggacgtgg gccagtggcg gcgcaatggc
      961 gccggcgcgc agatcctggc cgacctgggc ctgggcaagc tgcgcgtgct cggcaccccg
     1021 cggcggcagg tgggcctggc cggctacggg ctggaggtgg tcgagaccat cgcggccacg
     1081 gcgccggcgg ccgcgcggta aactgcgcgg ccccgttcca ggccgacccg ccatgccgta
     1141 catcgaaggc gacctgcgcg ccccgcaggg cgcgcgcttc gcgatcatcg ccagccgctg
     1201 gaacccgcgc atcgtcgatg cgctggtgga tgccgcgcgc gcgaccttca tcggcaacgg
     1261 cgtggacgaa gcggcggtgg acgtggtccg ggtccccggg gcatgggaat tgccgatggc
     1321 cgcgcgcgac atcgccctgg ccggcaggca tgcggccatc gtcgcgctgg gctgcgtggt
     1381 ccgcggcgac acgcgccact acgaacaggt ggccgatggc gcgtccgacg gattgatgcg
     1441 cgtcgccctg gacaccggcg tgccggtgct caacggcgtg ctggcggtgg atcgcttcga
     1501 ggatgccgcg gcacgcgccg gcggcagcca tggcaacaag ggcgaggagg ccgcactggt
     1561 ggcgatcgaa atggcgaacc tgcgggagaa actggcatga accggcatcg caacgatggc
     1621 atcgaccccg tggcccgctc gcgttcgcgt cggcgcgcct tgcaggcgat ctacgcctgg
     1681 cagctgagcg cgaccagcga aggcgccctc atcgcggaat tcgcccacga acaggcgcac
     1741 gagatcgccg accttgagta cttcgaggac ctggtgcgtg gcgtgttgcg ccacgtggcg
     1801 caactggacc aggcgctggc gggcttcatc gaccgcaagg tggacgaggt cgacccgatc
     1861 gagcgcgccg ccctgcgcat cgccgcctac gaactgctgc accgccccga cgtgccctac
     1921 cgggtggtga tcaacgaagc gatcgactcc accaagcgct tcggctccga gcacggccac
     1981 acctacgtca acggcgtgct ggacaaggcc gcgctggcgt tgcgcggcag cgagtcgcag
     2041 gcggcgcgcc cgcgctgaac cacggaacgg cggtggacag cacggtcgcc gaattcgacc
     2101 tgatcgagcg catccgccgg cgcgtcgcgg cccgccccga cgtgctgctc ggcatcggcg
     2161 acgatgccgc gctgctgcag ccaccggtcg gcatgcagct ggcggtggcg atggacacgc
     2221 tcaacagcgg cgtgcatttc ccgccggaga ccgcgcccgc cgacatcggc tggaaggcgc
     2281 tggcggtgaa tctctccgac ctcgcggcga tgggcgcatc gccggcgtgg tgcacgttgt
     2341 cgctgtccct gcccgatgca tcgaccgggt tcgtcgaggg gttcctggac gggttcctcg
     2401 cgctcgccgc gcaccatgac gtcgcgcttg tgggtggcga caccacgcgc gggccgttgt
     2461 cggtttgcgt ggcggtgcac gggttcatcg agccgggcca ggcgttgcgg cgcgatggcg
     2521 cctgcgttgg cgacgacctc tgggtcagcg gcaccctggg cgatgccgcg gcggcgctgt
     2581 cgcagtggca ggcaggcggc gcgatcgatc cggccttgcg cctgcgcctg gaccggccta
     2641 cgccgcgggt gtcgcttgga cgcgcgctgc gcggcatcgc ggcctcctgc atcgatgtct
     2701 cggatggcct gctggcggac ctggggcatg tctgcaaggc aagcggcgtc ggcgcgtggc
     2761 tcgacgtgga tgggttgccg gcgtcgcccg tgttgcgagc gagcttcgac ggcgaggcgc
     2821 gccgacggct gcaggccacc ggcggcgacg actacgaact gtgtttcagc gcgccggcca
     2881 gcatgcgcga agcggtgctc gaagcagcct ccgcgcacgt cccggtgacg cgcatcggtc
     2941 gcgtcgttgc cggtaccggc gtgtccgccg tggatcgaca tggcatggct tgggcgcgat
     3001 ccagcgaagg gttccggcac ttcgagtaag cgcttgcatc gctgccgtgc cggacgtcat
     3061 cccgacctcg cgcactgtgc gtgcctacgg gcgaccgtgc accttgggcg ccacatccgc
     3121 gcaggcaagg gaacaacaat gggcaggaaa ttcctcgtgg gcatggcact ggtggcgatc
     3181 ctcggcgcag tgggcgcgag cggctaccag ttcggcaagt tcctcaagga gcgcgagcgc
     3241 gccgaggcgg cgcgaccggt ctaacgtcct cgtcttccag gttcctgcac cggatcccgg
     3301 ccttccacgt ccttcgcctc gtccttcggg tcgggtaggg ccttcaccaa gggttcgatc
     3361 agggcgacgg gaaccacggt gaccttgttg tcgccggcga gcgcggattg cgtctccaat
     3421 ggagttgccg cggacaccgc cgcgttcacc ggaagcagcg gggcgttcat ccgcagcgcc
     3481 ttcggcgctg
//
LOCUS       DAOOCX010000206         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_26020_length_3487_cov_8.983974, whole genome shotgun sequence.
ACCESSION   DAOOCX010000206 DAOOCX010000000
VERSION     DAOOCX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26020_length_3487_cov_8.983974"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PK399_12460"
     CDS             complement(<1..227)
                     /locus_tag="PK399_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW13974.1"
                     /translation="MNTRYNAADIEVLSGLDPVKRRPGMYTDTARPNHLAQEVIDNAV
                     DEALAGHARTISVTMHEDGSCEVSDDGRGMPV"
     gene            376..1392
                     /locus_tag="PK399_12465"
     CDS             376..1392
                     /locus_tag="PK399_12465"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HPW13975.1"
                     /translation="MDVAARAAGNQPGARLIAADVGGTYARLGLVGVDAGGGISVLFH
                     RRYPCAVFAGLAAILRRFREECEAAQAGPFPVACVVAIAGVLQGDQLLNSNLPWKVSL
                     AETRREAGIAHLHLINDFEALAWALPQVPVDQLQVLGAGDGNGLRLPVVLVGPGTGLG
                     AALVIDQGGRTGVQPSEAGHAALAANTPLELDVVRLLQARFGHVDNERVLSGPGLINL
                     YHALCELRGVQPHWHTPSELVDAADAGSDLLAAECAALFCRWLGSFAGDLAITFRAGS
                     VCLAGGLSGHLDRYLRQGGFMQRFLDKGVLSDTLRDVPVYTVEHGQLGIIGAAAWHVA
                     HHPS"
     gene            1406..2407
                     /locus_tag="PK399_12470"
     CDS             1406..2407
                     /locus_tag="PK399_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HPW13976.1"
                     /translation="MSSRRRFLQSSLMAAGAALLPAARALPASNGAKVVSTWDFGVPA
                     NREAWRILAAGGNALDAVEAGARWAETGQCNSTVGRCGYPDRDGFVTLDASIMKGDGS
                     CGAVAALQDIDHPISVARKVMEKTPHVLLVGDGARQFAVAEGFEPRELRTPEAEKAWR
                     EWRETSRYAPEINAERRGVPGNKDNHDTIGMLALGADGRMAGACTTSGMAWKMHGRVG
                     DSPIIGAGLYVDDQVGGATASGVGEEMLRNAASFLVVELMRQGHSPSDACHLAIERVV
                     MKRPQASKTLQVCFLAMNNHGEVGAFALHQGFVYAVCDAQQQDTLLQAPAVYATSKT"
     gene            2407..3138
                     /locus_tag="PK399_12475"
     CDS             2407..3138
                     /locus_tag="PK399_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HPW13977.1"
                     /translation="MASSRRLLEIAANSVESALAAQAGGADRVELCANLGEGGTTPSF
                     GSIAFARDRLRIPLHVLVRARAGDFTCSPAELEAMRRDVEACVRLGCDGVVIGLLDIA
                     GHVDLAGCRVLIEAAGGLDVTFHRAFDVVADRTTAMERIIGLGCSRILSSGGADHALD
                     GAAVIAADIERAAGRIALVAGAGVTAGNIADVARLAGARELHASARVFDPGIGAEGPA
                     GLARGHGRSDEAVVRALREALDAES"
     gene            3255..>3487
                     /gene="pyrG"
                     /locus_tag="PK399_12480"
     CDS             3255..>3487
                     /gene="pyrG"
                     /locus_tag="PK399_12480"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268423.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HPW13978.1"
                     /translation="MTSPASPTPLVFVTGGVVSSLGKGIAAASLAAILEARGLKVTMM
                     KLDPYLNVDPGTMSPFQHGEVYVTDDGAETDLDL"
BASE COUNT          472 a         1184 c         1252 g          579 t
ORIGIN      
        1 accggcatgc cgcggccgtc gtcggacacc tcgcagctgc cgtcttcatg catggtcacg
       61 ctgatcgtgc gcgcatgtcc tgccagcgcc tcgtccaccg cgttgtcgat gacttcctgg
      121 gccaggtggt tgggccgcgc ggtgtcggtg tacatgccgg ggcggcgctt gaccggatcc
      181 aggccggaga ggacttcgat gtcggcggcg ttgtagcggg tattcattcg ttcgatcgag
      241 tcgtgcaacg tgcagggggc ggccagtgtg tggcctgagg tcgggatttg cacgccagcc
      301 ctgcctgcct tgcggccgtg gccgatacaa tcgcggaaag gccaggcgca tcgccggcca
      361 gggagcttgc ctgtggtgga tgttgccgca cgcgcggccg ggaaccagcc cggcgcgcgg
      421 ttgatcgctg ccgacgtggg cggcacctac gcccggctgg gcctggtcgg ggtggacgcc
      481 ggtggcggca tcagcgtgct gttccaccgc cgctatccct gcgcggtatt cgccggcctg
      541 gccgcgatcc tccgccgctt ccgcgaggaa tgcgaggctg cgcaggccgg cccgttcccg
      601 gtggcctgcg tggtcgccat cgccggcgtg ctgcagggcg accagttgtt gaacagcaac
      661 ctgccgtgga aggtctcgct ggccgagacc cgccgcgagg ccggcatcgc ccacctgcac
      721 ctgatcaacg atttcgaggc gctggcctgg gccctgccgc aggtcccggt cgatcaattg
      781 caggtgctgg gtgccggcga cggcaatgga ttgcgcctgc cggtggtgct ggtcgggccc
      841 ggcaccgggt tgggcgcggc cctggtgatc gaccagggtg gccgcacggg cgtgcagccc
      901 agtgaggccg ggcatgccgc actggcggcg aataccccgc tggaactcga cgtggtgcgc
      961 ctgctgcagg cgcgcttcgg ccacgtggac aacgagcgcg tgctctcggg ccccggactg
     1021 atcaacctgt accacgcctt gtgcgaactg cgcggcgtgc agccgcactg gcatacgccc
     1081 tccgaactcg tcgatgccgc ggatgccggc agcgacctgc tggccgccga gtgcgccgcg
     1141 ctgttctgcc gctggctggg cagcttcgcc ggcgacctgg ccatcacgtt ccgcgccggt
     1201 tcggtgtgcc tggccggcgg tctgtccgga cacctggacc gttacctgcg ccagggcggc
     1261 ttcatgcagc gcttcctcga caagggcgtg ctctccgaca ccctgcgcga cgtgccggtg
     1321 tacacggtcg aacacggcca gctcggcatc atcggcgccg ctgcgtggca cgtcgcccac
     1381 catccttcct gacagggcca tcacgatgtc gtcgcgccgc cgcttcctgc agtcttcgtt
     1441 gatggccgcg ggtgcggcgc tgttgccggc tgcgcgcgca ttgccggcat cgaatggggc
     1501 aaaagtcgtc tccacctggg atttcggcgt gccggccaat cgcgaggcat ggcgcatcct
     1561 ggcggctggc ggcaatgcac tcgatgcggt cgaggccggc gcgcgctggg cggagaccgg
     1621 ccagtgcaat tccaccgtcg gccgttgcgg ctatccggat cgcgacggct tcgtgaccct
     1681 ggatgccagc atcatgaagg gcgacggcag ctgcggtgcg gtggcggcct tgcaggacat
     1741 cgaccacccg atctcggttg cccgcaaggt gatggagaaa acgccgcatg tgttgctggt
     1801 cggcgatggc gccaggcagt tcgcggttgc cgaaggcttc gaaccgcgcg agctgcgcac
     1861 gccggaagcg gagaaggcgt ggcgcgaatg gcgggagacc tcgcgctatg cgccggagat
     1921 caacgccgaa cgccgcggcg taccgggcaa caaggacaac cacgacacca tcggcatgct
     1981 cgcgctcggt gccgatggcc gcatggcagg tgcctgcacc accagcggca tggcctggaa
     2041 gatgcacggc cgcgtcggcg acagtccgat catcggtgcc ggcctgtacg tggacgatca
     2101 ggtcggcggc gccaccgcct ccggcgtggg cgaggaaatg ttgcgcaatg ccgcgagttt
     2161 cctggtcgtc gaactgatgc ggcaaggcca ctcgcccagc gatgcctgcc acctggccat
     2221 cgaacgcgtg gtgatgaaac gcccgcaggc cagcaagaca ttgcaggtct gtttccttgc
     2281 gatgaacaac catggcgagg tcggcgcgtt cgcgctgcac cagggcttcg tttacgcggt
     2341 ctgcgatgcg caacagcagg acacgctgct gcaggccccc gcggtctacg ccacgtccaa
     2401 gacctgatgg cgtcgtcgcg gcggctgctg gagatcgcgg cgaattcggt cgaatccgcg
     2461 ctggccgcgc aggccggtgg cgccgaccgg gtggagcttt gcgccaacct cggcgaaggc
     2521 ggcaccaccc cgtccttcgg cagcatcgcc ttcgcccgcg atcgcctgcg catcccgctg
     2581 cacgtgctgg ttcgcgcgcg cgccggtgat ttcacctgtt cgcccgccga gctggaggcg
     2641 atgcgccgcg atgtcgaggc ctgcgtgcgg ctgggctgcg acggcgtcgt gattggcctg
     2701 ctggacatcg cggggcatgt cgacctggcc ggctgccggg tgttgatcga ggctgcgggc
     2761 ggcctggacg tgaccttcca ccgcgccttc gacgtggtcg cggatcgcac cacggcgatg
     2821 gaacgcatca tcggcctggg ctgctcgcgc atcctgagct ccggtggcgc ggaccacgcg
     2881 ctggacgggg ctgccgtgat cgccgccgac atcgagcgtg cggccggacg catcgccctg
     2941 gtcgccggtg ccggggtgac cgcgggcaat atcgccgacg tggcgcgcct ggcgggcgcc
     3001 cgcgaactgc atgcatcggc cagggtgttc gatcccggca tcggtgcgga ggggcctgcg
     3061 ggcctggccc gcgggcacgg gcgcagcgac gaagcggtgg tgcgcgcgct gcgcgaggcg
     3121 ctcgacgccg aatcctgagt cggccgggtt ttcccgttgc ggatcaatgg tttccgtcga
     3181 tacggacgca gccggcgcgt gcagcggcgg ggcgcttggc ctagaatgcg gctttccagc
     3241 gccgtgtcga cgccatgact tcccccgcat cgcctacccc cctcgtcttc gtcaccgggg
     3301 gcgtggtgtc ctcgctcggc aagggcatcg ccgccgcctc gctcgcggcg atcctcgaag
     3361 cccgtggcct caaggtcacg atgatgaagc tggacccgta cctcaacgtg gatccgggca
     3421 ccatgagccc gttccagcac ggcgaggtct acgtcaccga cgacggcgcc gagaccgacc
     3481 tcgacct
//
LOCUS       DAOOCX010000207         3455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_26335_length_3455_cov_9.266176, whole genome shotgun sequence.
ACCESSION   DAOOCX010000207 DAOOCX010000000
VERSION     DAOOCX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26335_length_3455_cov_9.266176"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PK399_12485"
     CDS             complement(<1..377)
                     /locus_tag="PK399_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HPW13979.1"
                     /translation="MHAPPAPRRPRPGLPEDGWALFLDVDGCLLEFADAPDRVIVPLG
                     LRECLHALSERLGGALALVSGRSLEALDDLFWPLRLPAAGLHGLERRSRNAEHRAPIA
                     THVLRRLCAQAETLAIEHRGAMV"
     gene            complement(402..707)
                     /locus_tag="PK399_12490"
     CDS             complement(402..707)
                     /locus_tag="PK399_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005915765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPW13980.1"
                     /translation="MTAGWHLYLLRCRDGSVYAGITTDIDRRFRQHASGRGAKYTRAH
                     PPECVLGSRGYPDRASASRAEHELKRQPKARKLAWLLADPQPRSAEDGAPPDASTRC"
     gene            complement(704..2014)
                     /locus_tag="PK399_12495"
     CDS             complement(704..2014)
                     /locus_tag="PK399_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPW13981.1"
                     /translation="MIRSRVLAHGLLLVLSAAPCFGATPSPGEQRMRAAIDADQPHAI
                     ALLEKLVNQNSGSLNIPGVTAVGGMVRAELEPLGFDVQWIDMRATGRAGHLVATHAGN
                     GRGKRILMIGHLDTVFEPDSTFQRFVRDGERATGPGIADDKGGVVIIIAALRAMHAAG
                     TLRDADIKVVLTGDEESAGTPLEAARADLVAAGKWADVALEYEELIVRDGRDYATVAR
                     RGVTDWELTTTGITGHSGGVFGDALGYGANYELVRILDAFRRELPEPNLTVNVGLMLG
                     GTPATLDPGGARGSAAGKTNIVAAEAVARGDMRTFSAAQEARARAKMQAIVDAHLPKT
                     DATLAFDDSYPAMAPTAGNRALLALLNQVNADLGLPAMGEWDPAKRGAADSSFVAADA
                     DVLAGLGAAGTGAHAVGETLDLASIPRQALRSAVLIERLSRTPR"
     gene            2153..>3455
                     /locus_tag="PK399_12500"
     CDS             2153..>3455
                     /locus_tag="PK399_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPW13982.1"
                     /translation="MRMDKLTSRFQQAIADAQSLAVGRDHTIIEPAHLLLALLDQQGG
                     GTRPLLAQAGVNVPVLRERLTEALDKLPKVSGQAGNVQAGNDLVRLLNVTDKLAQQRG
                     DAYIASELFLLAALEESGAAGKALKAAGGDKARLEAAIDKLRGGEKVQDENAEDARQA
                     LEKYTIDMTARAESGKFDPVVGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLRGKRLLSLDMGALIAGAKFRGEFEERLKAVLNDLSKNEGQIILF
                     IDELHTMVGAGKAEGSMDAGNMLKPALARGELHCIGATTLDEYRKYVEKDAALERRFQ
                     KVFVGEPSVEDTIAILRGLKERYAVHHGVEITDPAIVAAATLSNRYIADRQLPDKAID
                     LMDEAASRIRMEIDSKPEEMDRKERRLIQLKIQREALKKEKD"
BASE COUNT          578 a         1161 c         1225 g          491 t
ORIGIN      
        1 tccaccatcg cgccacgatg ctcgatcgcc agcgtctcgg cctgtgcgca caggcggcgc
       61 agcacatggg ttgcgatggg cgcgcgatgt tcggcattgc gcgagcggcg ctccagtccg
      121 tgcagtcccg ccgccggcaa gcgcagtggc cagaacaggt cgtccagcgc ttccagggaa
      181 cgaccactga ccagcgccag ggcgccaccc aagcgctcgg acaacgcatg gaggcattcc
      241 cggagtccga gcggcacgat cacccggtct ggggcatcgg cgaactccag gagacagcca
      301 tcgacgtcga ggaacagggc ccagccatct tcgggcaggc ccggacgcgg ccgtcgtggg
      361 gcgggcggtg cgtgcatcgg gccagtatgg ccagcccggt gttagcagcg ggtcgacgcg
      421 tcgggtgggg cgccgtcctc tgccgatcgt ggctgcggat cggccagcaa ccacgccagc
      481 ttgcgcgcct tcggctggcg cttgagctca tgctcggcgc gcgaggcgct ggcgcgatcc
      541 gggtagccgc ggctgccgag cacgcattcc ggtggatgcg cgcgggtgta cttcgcgccg
      601 cgtcccgacg cgtgctggcg gaagcggcgg tcgatgtcgg tggtgatgcc tgcatacacg
      661 ctgccgtcgc ggcagcgcag caggtacagg tgccagccgg ccgtcatcgc ggcgtgcgcg
      721 acaatcgctc gatcagcacc gcgctgcgca gggcctggcg cggaatgctg gccaggtcga
      781 gcgtctcccc caccgcgtgc gcgccggtgc ctgctgcgcc caggcccgcg agcacgtcgg
      841 cgtcggcggc gacgaagctg gagtcggcgg cgccgcgctt ggccggatcc cattcgccca
      901 tcgccggcag gccgaggtcg gcgttgacct ggttgagcag ggcgagcagg gcccggttgc
      961 ccgcggtagg cgccatcgcc ggataggaat cgtcgaaggc gagggtggcg tcggtcttcg
     1021 gcaggtgggc gtcgacgatc gcctgcatct tcgcccgcgc ccgcgcctcc tgcgccgcgc
     1081 tgaaggtgcg catgtcgcca cgcgcgaccg cttccgcggc gacgatgttg gtcttgccgg
     1141 cggcgctgcc gcgcgcgccg cccgggtcca gcgtggcagg cgtgccgccg agcatcaggc
     1201 cgacgttgac ggtgaggttg ggttccggca gttcgcgccg gaatgcatcg aggatgcgca
     1261 ccagttcgta gttcgcgcca tagccaagcg cgtcgccgaa cacaccgccg gaatgtccgg
     1321 taatgccggt ggtggtgagt tcccagtcgg tcacgccgcg acgtgcaacc gtggcgtagt
     1381 cgcggccatc gcggacgatc agttcttcgt actccagggc aacgtccgcc cacttgcccg
     1441 ccgcgaccag gtcggcgcgc gcggcctcca gcggcgtgcc ggccgactcc tcgtcgccgg
     1501 tcagcaccac cttgatgtcg gcatcgcgca gggtgccggc ggcatgcatg gcgcgcaatg
     1561 cagcgatgat gatcaccacg ccgcccttgt cgtcggcgat gccgggaccg gtcgcgcgtt
     1621 cgccgtcgcg gacgaagcgc tggaacgtcg aatccggctc gaacacggtg tccaggtggc
     1681 cgatcatcag gatccgcttg ccgcgtccgt tacccgcgtg ggtggcgacc aggtggccgg
     1741 cgcgtccggt agcgcgcatg tcgatccact gcacgtcgaa gcccagcggc tccagctccg
     1801 cgcgcaccat tccgccgacc gcggtgaccc cgggaatgtt gagcgagccc gagttctggt
     1861 tgaccagctt ctccagcaag gcgatcgcat gtggctggtc ggcgtcgatg gcggcacgca
     1921 tccgctgctc gcccggggac ggcgtggcgc caaagcaggg tgcggcggac aggaccagca
     1981 gcaagccatg tgcaaggacg cgggaacgga tcatcgggcg ggttccggga cgggcagccg
     2041 agaccataac ccagcccgaa tgccgcgcgc gctttgactt gaatcagggc aaggcaaccg
     2101 catctggaac acattggccg cgagcggccc atccctcttc aaggattccc ccatgcgcat
     2161 ggacaaactc acctcgcgct tccagcaggc catcgccgac gcgcagtcgc tggccgtggg
     2221 tcgcgaccac accatcatcg aacccgcgca cctgctgttg gcgctgctgg accagcaggg
     2281 tgggggcacg cgcccgctgc tggcgcaggc cggcgtcaac gtgccggtgc tgcgcgagcg
     2341 cctgaccgag gccctcgaca aactgccgaa ggtcagcggc caggccggca acgtgcaggc
     2401 cggcaacgac ctggtgcgcc tgctcaatgt caccgacaaa ctggcccagc agcgtggtga
     2461 tgcctacatc gccagcgagc tgttcctcct cgctgcactg gaggagtcag gcgcggccgg
     2521 caaggcgctg aaggccgccg gcggcgacaa ggccaggctg gaggcggcca tcgacaagtt
     2581 gcgtggtggc gagaaagtgc aggacgagaa cgccgaggac gcgcgccagg cgctggagaa
     2641 atacacgatc gacatgactg cgcgcgcgga gagcggcaaa ttcgatccgg tggtcggccg
     2701 cgacgaggaa atccgtcgca ccatccaggt cctgcaacgg cgcaccaaga acaacccggt
     2761 gctgatcggc gagcccggcg tcggcaagac cgcgatcgtg gagggccttg cccagcgcat
     2821 catcaacggc gaggtgccgg aaggcctgcg aggcaagcgt ctgctgtcgc tggacatggg
     2881 cgcgctgatc gccggcgcca agttccgcgg cgagttcgag gagcgcctga aggcggtgct
     2941 caacgacctg tcgaagaacg aaggccagat catcctgttc atcgatgagc tgcacaccat
     3001 ggtcggcgcc ggcaaggcgg aaggctcgat ggatgccggc aacatgctca agcccgcgct
     3061 cgcacgcggc gagctgcact gcatcggcgc gaccacgctt gacgaatacc gcaagtacgt
     3121 ggagaaggac gccgcgctgg aacgccgctt ccagaaggtc ttcgtgggcg agccgtcggt
     3181 ggaggacacc attgcgatcc tgcgcggact gaaggaacgc tacgcggtgc accatggcgt
     3241 cgagatcacc gatcccgcca tcgtcgccgc ggccacgctg tccaaccgct acatcgccga
     3301 ccgccagttg ccggacaagg cgatcgacct gatggacgaa gcggcatcgc gcatccgcat
     3361 ggagatcgat tccaagccgg aggagatgga ccgcaaggaa cgccggctga tccagctcaa
     3421 gatccagcgc gaggcgctga agaaggagaa ggacg
//
LOCUS       DAOOCX010000208         3442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_26473_length_3442_cov_8.531739, whole genome shotgun sequence.
ACCESSION   DAOOCX010000208 DAOOCX010000000
VERSION     DAOOCX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26473_length_3442_cov_8.531739"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /gene="ilvD"
                     /locus_tag="PK399_12505"
     CDS             <1..1530
                     /gene="ilvD"
                     /locus_tag="PK399_12505"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPW13983.1"
                     /translation="AHCADALVCISNCDKITPGMLMAALRLNIPTVFVSGGPMEAGKT
                     KLADGTTSGHKLDLIDAMVMAADPNASDEEVAVVERSACPTCGSCSGMFTANSMNCLT
                     EALGLSLPGNGTVLATHADREQLFLQAGRTAVELCHRWYGAEDARALPRGIATFEAFE
                     NAMTLDIAMGGSTNTILHLLAAAQEAEVAFGMADIDRLSKRVPQLCKVAPNTQKYHIE
                     DVHRAGGVIAILGELARGGLLHTDVATVHAATLGDAIAKWDIATVDDERVSTFYRAGP
                     AGIPTQVAFSQATRWPSLDADRAEGCIRSVAHAYSQEGGLAVLAGNIAPDGCVVKTAG
                     VDASVHVFEGTARVYESQEAAVAGILGDEVQAGDVVVIRYEGPKGGPGMQEMLYPTSY
                     LKSKGLGKACALLTDGRFSGGTSGLSIGHASPEAAAGGAIGLVRDGDRIRIDIPARSI
                     NLVVDEDELAARRAEQDAKGWKPVQPRARKVSTALKAYALLATSADKGAVRNTAMLDA
                     T"
     gene            1654..2160
                     /locus_tag="PK399_12510"
     CDS             1654..2160
                     /locus_tag="PK399_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW13984.1"
                     /translation="MGIGFRGLSLVMLAALPLGATAGDTIKVARVVPYMRDISDTNLH
                     KCDWNRNLPGLISWKSGRTVEVTDADLATVEGKVLVLSIVKAHTAGGGNMSGPKWGQV
                     RGELRENGELLGNFSLRRVSARPFDMAVCKPLDRIAVALAGDIAAWLKHPTINPDADK
                     VAEPAPAE"
     gene            2289..>3442
                     /locus_tag="PK399_12515"
     CDS             2289..>3442
                     /locus_tag="PK399_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPW13985.1"
                     /translation="MRPLHLLLSFALLLAGCASQPAVVAAAPATHGAAHAPHARPKMQ
                     SGFVQVEGTRFTIDGNPYRFAGANFWYGAYLGAPDGIGDRARLRAELDQLKAAGIDNL
                     RVLAMSEASGFRRAVRPAIMTAPGTYDERLLQGLDTLLVEMGKRDMKAVLYLNNFWQW
                     SGGMSRYVAWATGEPLSDPDASGDWDGFMQNSARFYALPKAQAIYRDAIRTIVGRTNG
                     ISGVAYADDPTVMSWQLANEPRPGSAKDGHLNLAAYDTWIDETAGFIHGLAPKQLVST
                     GSEGAMGSLGDLDLFLQAHKTPNVDYLTFHLWPSNWSWIDHKDPAARLESGLKASLDY
                     IDKHIDAAGKLGKPIVLSEFGLNRDGGSYDAASGTTARDRFYRAIYARLL"
BASE COUNT          589 a         1112 c         1186 g          555 t
ORIGIN      
        1 gcgcattgcg cggacgcgct ggtgtgcatt tccaactgcg acaagatcac gcccggcatg
       61 ctgatggcgg cactgcgact caacatcccg accgtgttcg tgtccggcgg gccgatggaa
      121 gccggcaaga ccaaactcgc cgatggcacc acgtccggcc acaagctcga cctgatcgac
      181 gcgatggtga tggccgcgga tccgaatgcg tccgacgaag aagtggccgt tgtcgaacgc
      241 agtgcgtgcc cgacctgcgg atcctgcagc ggcatgttca ccgcgaactc gatgaactgt
      301 ttgaccgaag cattgggcct gtcgttgcct ggcaacggca cggtgctggc gacgcatgcg
      361 gatcgcgagc agttgttcct gcaagccggg cgcactgcgg tggaactctg ccatcgctgg
      421 tatggcgccg aggatgcgcg cgccctgccg cgcggcatcg ccaccttcga agcgttcgag
      481 aacgcgatga cactcgatat cgcgatgggc ggttcgacca acacgatcct gcacctgctc
      541 gctgccgcgc aggaggccga agtcgccttc ggcatggccg acatcgaccg gctgtcgaaa
      601 cgcgtgccgc aactgtgcaa ggtcgcgccg aatacccaga agtaccacat cgaggacgtg
      661 catcgcgccg gtggtgtgat cgcgatcctc ggcgaactcg cgcgtggcgg cttgctgcac
      721 accgatgtcg cgaccgtgca tgcagcgacc ctgggcgatg cgatcgcgaa gtgggacatc
      781 gcgaccgtcg acgacgaacg ggtatcgacg ttctatcgcg ccggcccggc cggcatcccg
      841 acccaggtcg ccttcagcca ggccacgcgc tggccgtcgc tggatgcgga tcgcgctgag
      901 ggctgcatcc gcagtgtcgc gcatgcgtat tcgcaggaag gcgggctcgc cgtgctggcc
      961 ggcaacatcg cgcccgatgg ctgcgtggtg aagacggcag gcgtggacgc atccgtgcat
     1021 gtattcgaag ggacggcacg cgtctatgaa agccaggagg cggcggtggc cggcatcctc
     1081 ggcgacgagg tgcaggccgg cgacgtggtg gtgatccgct acgaaggccc gaagggtggg
     1141 ccgggcatgc aggagatgct ctacccgacc agttacctga agtcgaaagg gctcggcaag
     1201 gcgtgcgcgt tgctcaccga tggccgcttc tccggcggca cctcgggcct gtcgatcggc
     1261 catgcgtcgc cggaggccgc ggcgggtggc gcgatcggcc tggtccgcga tggcgaccgc
     1321 atccgcatcg atattccggc gcgcagcatc aacctggtgg tggatgaaga tgagcttgcc
     1381 gcgcgacgcg ccgagcaaga cgccaaaggc tggaagccgg tgcagccgcg cgcgcgcaag
     1441 gtcagcaccg cgctcaaggc ctatgcgctg ctggcgacga gcgccgacaa gggtgcagta
     1501 cgcaatacgg cgatgctcga cgcgacctga gtcgcattcc ggcgtcgcgc ggattgccgc
     1561 gcacgcccgc cgtcacggac aatccgcggc cttgccgggg acgccccggc gtctcgcggc
     1621 catgtgccgc gcaatggatc ggggatcgtt cggatgggaa tcgggtttcg cgggttgtcg
     1681 ctggtcatgc ttgccgcctt gccgctgggg gcgaccgcgg gcgacaccat caaggtcgcc
     1741 agggtcgtgc cctacatgcg cgacatcagc gataccaacc tgcacaagtg cgactggaac
     1801 cggaacctgc cggggctgat ctcgtggaaa tcgggtcgca cggtcgaagt caccgatgcc
     1861 gacctcgcta ccgtcgaagg caaggtgctg gtgctgtcca tcgtgaaggc gcataccgca
     1921 ggcggcggca acatgagcgg cccgaaatgg gggcaggtgc gaggggagtt gcgcgaaaac
     1981 ggcgagttgc tcggcaactt cagtctccgc cgggtatcgg cgcggccgtt cgacatggcc
     2041 gtgtgcaagc cgctggacag gatcgccgtg gcgctggcgg gcgacatcgc tgcgtggctg
     2101 aagcatccca ccatcaaccc ggatgctgac aaggtggcgg agcccgcacc cgccgagtga
     2161 cgcgcgccag caccgtggcc gcgcgccgtg aggcatgacg aaacacgcgc cggctatgtc
     2221 gtgattggca atggtcgcgg ccggcgcgct cgactatcgt gcgggcttgt ctccaggcag
     2281 gcccgaccat gcgccccttg cacttgctgc tgtcgttcgc gttgttgctg gccggctgcg
     2341 cgtcgcaacc ggctgtcgtc gctgctgcgc ctgccacgca cggcgccgcg cacgcgccgc
     2401 acgcgcgccc gaagatgcag tccggcttcg tgcaagtcga gggcacccgt ttcaccatcg
     2461 atggaaatcc gtatcgcttc gcgggtgcga acttctggta tggcgcgtat ctcggtgcgc
     2521 ccgacggtat cggcgaccgt gcgcggctgc gcgccgaact cgaccagctg aaggcagccg
     2581 gcatcgacaa cctgcgcgtg ttggcgatgt ccgaagccag tggcttcagg cgcgcggtgc
     2641 gtcccgcgat catgaccgcg ccgggcacgt acgacgaacg gctgctgcag gggctcgaca
     2701 ccctgctggt ggaaatgggc aagcgcgaca tgaaggccgt gctctacctc aacaatttct
     2761 ggcaatggtc cggcggcatg tcgcggtacg tggcatgggc gaccggcgag ccgctttccg
     2821 accccgacgc cagcggcgac tgggatggct tcatgcagaa ttcggcgcgc ttctacgcgc
     2881 tgccgaaggc gcaggcgatc tatcgcgacg cgatccgcac catcgttggc cgcaccaatg
     2941 gcatcagcgg cgtggcgtat gccgatgatc cgacggtgat gtcctggcaa ctggcgaatg
     3001 agccgcgtcc gggcagtgcg aaggatggcc acctcaatct cgccgcgtac gacacatgga
     3061 tcgatgagac cgcgggtttc atccacggat tggcgccgaa acaactggtc agcaccggca
     3121 gcgaaggcgc gatgggctcg ctcggcgacc tcgacctgtt cctgcaggcg cacaagacgc
     3181 cgaacgtcga ttacctcacc ttccacctgt ggccgtcgaa ctggagctgg atcgaccaca
     3241 aggatcccgc ggcgcggctg gaatcaggcc tgaaggcctc gctggactac atcgacaagc
     3301 acatagacgc ggccggcaag ctgggcaagc ccatcgtgct gtcggagttc ggcttgaacc
     3361 gcgacggcgg atcctacgat gcggcgtcgg gcacgactgc gcgcgatcgg ttctaccgcg
     3421 cgatctacgc ccgcctgttg ca
//
LOCUS       DAOOCX010000209         3375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_27188_length_3375_cov_10.563855, whole genome shotgun
            sequence.
ACCESSION   DAOOCX010000209 DAOOCX010000000
VERSION     DAOOCX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27188_length_3375_cov_10.563855"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PK399_12520"
     CDS             <1..924
                     /locus_tag="PK399_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPW13986.1"
                     /translation="ADESDGSFLRLNPAVAIVTNIDADHLENYGGDFARVKAAFNEFL
                     HRLPFYGLAVLCIDDAEVAALAENTPRHVVTYGFSAAADVRAEDVRQQGGRMHYTLCL
                     PDGTRTPCTLALPGRHNVQNALAASAVAWQLGVHNDAIARALEQFAGIGRRFNQLAKL
                     PLDAGGDVVLVDDYGHHPKELAAVFDAARGGWPEKRLVVAFQPHRYSRTRDLFDDFAA
                     VLSGVDALVLTEVYPAGEAPIAGADAKSLARSIRTRGRVDPVVVNGAADLRDALVGVL
                     RDGDLLLMMGAGDIGAAAQQIARTGLQGDNA"
     gene            921..1904
                     /locus_tag="PK399_12525"
     CDS             921..1904
                     /locus_tag="PK399_12525"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPW13987.1"
                     /translation="MSADMPFPPLRVSDARDFGRVAVLMGGTSSEREVSLDSGRNVLE
                     ALQARGVDAHAVDGIPALVDALVHGQFDRVFNILHGNRGGGEDGVLQGLLEALEVPYT
                     GCGVLASALTMDKIRTKQVWMSAGLPTPKFLRLSPGADVRAAALQLGLPVFVKPSSEG
                     SSVGVARVTDEAGIDDAIRVARAYGGEMLMEQMVAGDELTVGILGDVALPSIRIVPKG
                     EWYDYNAKYIADDTQYLCPGLQGDDEAAIRQLALDAFRAAGCTGWGRVDVMRDRASGA
                     LLLLEVNTAPGMTSHSLVPKAAGQRGVSFQELCWRILEQTLPVVDGERADA"
     gene            1901..2698
                     /locus_tag="PK399_12530"
     CDS             1901..2698
                     /locus_tag="PK399_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HPW13988.1"
                     /translation="MSAMLRLLAWILAVALVALPVVAVVNGWVGGAQWPLRTLRVQGD
                     LHQVDQARLRATVLPYAKQGFFAVELDRIQAAVGALPWVERAEVRKHWPDVLEVRISE
                     HRPYARWGSDRLLSEHGKLFSAKGVQVRAGLPQLDGPDTQVAEVVALYNQARERLANA
                     GGVRGAGMDKRGSWSITLSDGTEVVLGRSDPEQRLERFAPLLPRLAAQHPGQRLVRAD
                     LRYTNGFALSWAALPKASALPAPVQDATLPQPAPVAPAGRNDTQGTT"
     gene            2695..>3375
                     /gene="ftsA"
                     /locus_tag="PK399_12535"
     CDS             2695..>3375
                     /gene="ftsA"
                     /locus_tag="PK399_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPW13989.1"
                     /translation="MNRKGDKSLIVGLDIGTSKVTALVGEYAPGEPIEVIGIGSHESR
                     GLRKGVVVDIDSTVQSIQRAVEEAELMAGCEVRSVYASISGNHVQCRNSQGIAPIRDG
                     EVSYGDLDRVLEAAKAVAIPADQRILHAIPRDYVLDDSQEGIRNPVGMTGVRLEVHAH
                     LVVCAQSAAANITKCVQKCGLQVDDLVLSSLASSTAVLTPDERELGVVLVDMGAGTTD
                     IAVFVQGAI"
BASE COUNT          508 a         1131 c         1241 g          495 t
ORIGIN      
        1 gccgacgaga gcgacggcag cttcctgcgc ctgaacccgg cggtggcgat cgtcaccaac
       61 atcgacgccg atcacctgga gaactacggc ggcgacttcg cccgggtgaa ggcagcgttc
      121 aacgaattcc tgcaccggct gccgttctac gggctggcgg tgctgtgcat cgacgatgcc
      181 gaagtggccg cgctggccga aaacaccccg cgccacgtgg tgacctacgg cttcagcgcc
      241 gcggccgacg tgcgcgccga ggacgtgcgc cagcagggcg gtcgcatgca ctacacgctg
      301 tgcctgccgg atggcacgcg cacgccgtgc acgctggcat tgccgggccg gcacaacgtg
      361 cagaacgcgc tggccgcctc cgcggtcgcc tggcagctcg gcgtgcacaa cgatgccatc
      421 gcccgcgcgc tggaacagtt cgccggcatc ggtcggcgct tcaaccagtt ggcgaagctg
      481 ccgctggacg caggcggcga cgtggtgctg gtcgacgact acggccacca cccgaaggaa
      541 ctcgccgcgg tgttcgatgc cgcgcgcggc ggctggccgg agaagcggtt ggtggtcgcg
      601 ttccagccgc atcgctattc gcgcacccgc gacctgttcg acgatttcgc cgcggtgctg
      661 tccggtgtgg atgcgctggt gctgaccgag gtgtacccgg ccggcgaagc gccgatcgcc
      721 ggggccgatg cgaagtcgct cgcgcgctcg atccgcaccc gtggtcgggt cgatccggtc
      781 gtggtcaacg gtgccgccga cctgcgcgat gcgctcgtcg gcgtgctccg cgacggcgac
      841 ttgctgctga tgatgggtgc cggcgacatc ggcgcggccg cgcagcagat cgcacgcact
      901 ggcctgcaag gagacaacgc atgagcgcgg acatgccgtt cccgccgctg cgggtgagcg
      961 atgcgcgcga cttcggccgc gtcgccgtgc tgatgggcgg caccagcagc gaacgcgagg
     1021 tctcgctgga ttccggtcgc aacgtgctgg aggcgctgca ggcgcgcggc gtggatgcgc
     1081 atgcggtcga cgggatcccg gcgctggtcg acgcgctggt gcacggccag ttcgaccgcg
     1141 tcttcaacat cctccacggc aatcgtggcg gtggcgagga tggcgtgctg cagggcctgc
     1201 tggaagcgct ggaagtgccg tacaccggct gcggcgtgct ggccagcgca ctgaccatgg
     1261 acaagatccg caccaagcag gtgtggatga gcgcgggatt gccgacgccg aagttcctgc
     1321 gcctgtcgcc gggtgcggat gtgcgggccg cggcgctgca gctcggcttg cccgtattcg
     1381 tgaagccgtc cagcgaaggc tccagcgtgg gcgtggcgcg ggtcaccgac gaggccggca
     1441 tcgacgacgc gatccgcgtc gcgcgcgcct atggcggcga gatgctgatg gaacagatgg
     1501 tggccggcga cgaactcacc gtcggcatcc tcggcgacgt cgcgctgccg tcgatccgca
     1561 tcgtgcccaa gggcgagtgg tacgactaca acgccaagta catcgccgac gacacccaat
     1621 acctgtgccc cggcctgcag ggcgacgacg aggccgcgat ccgccagctc gcgctggacg
     1681 cgttccgggc cgcgggctgc accggctggg gccgcgtcga cgtgatgcgc gatcgcgcca
     1741 gcggcgcgct cctgctgctg gaagtgaaca ccgcgccggg catgaccagc cattcgctgg
     1801 tgccgaaggc ggccggccag cgtggcgtgt cgttccagga gctgtgctgg cggatcctcg
     1861 agcagaccct gcccgtggtc gacggggagc gcgcggacgc atgagcgcga tgctgcgact
     1921 gctggcctgg atcctcgcgg tggcgctggt cgcactgccg gtcgtggccg tcgtcaacgg
     1981 ttgggtgggc ggcgcgcaat ggcccctgcg cacgctgcgc gtgcagggcg acctgcacca
     2041 ggtcgaccag gcgcgactgc gcgcgaccgt gctgccgtat gcgaagcagg gcttcttcgc
     2101 ggtggagctg gatcgcatcc aggccgcggt cggcgcgttg ccgtgggtgg agcgcgcgga
     2161 agtgcgcaag cactggccgg acgtgctgga agtgcggatc agcgagcatc gtccgtacgc
     2221 gcgctggggc tcggatcgcc tgctctccga gcacggcaag ttgttctcgg cgaagggcgt
     2281 gcaggtgcgc gcgggcttgc cgcaactcga cggcccggac acccaggtgg ccgaagtcgt
     2341 ggccctctac aaccaggcgc gcgagcggtt ggccaatgcc ggcggcgtgc gcggcgccgg
     2401 catggacaag cgcggcagtt ggtcgatcac gttgtccgat ggcaccgaag tcgtgctcgg
     2461 gcgcagcgat cccgaacagc ggctggaacg cttcgcacca ctgttgccgc ggctggccgc
     2521 gcagcacccc gggcagcgcc tggtgcgcgc ggacctgcgc tacaccaatg gttttgcact
     2581 gagctgggcc gcgctgccga aggcgagcgc gctgcccgcg ccggtgcagg acgccactct
     2641 cccccaaccc gcgccggtgg caccggccgg gcgcaacgac acgcaaggaa ccacatgaac
     2701 cgcaagggcg acaagtcgct gatcgtcggc ctggacatcg ggacctccaa ggtcaccgcg
     2761 ctggtcggcg aatacgcgcc gggcgaaccg atcgaggtga tcgggatcgg ctcccacgaa
     2821 tcgcgcggcc tgcgcaaggg cgtggtggtc gacatcgatt ccaccgtgca gtcgatccag
     2881 cgcgcggtgg aagaagccga gctgatggcc ggctgcgaag tgcgcagcgt gtatgcctcg
     2941 atcagcggca accacgtgca gtgccgcaac tcgcagggca tcgccccgat ccgcgacggc
     3001 gaagtcagct acggcgacct cgaccgcgtg ctggaggcgg ccaaggcggt cgcgatcccg
     3061 gccgaccagc gcatcctgca tgcgatcccg cgcgattacg tgctggacga ttcgcaggaa
     3121 ggcatccgca acccggtcgg catgaccggc gtgcgcctgg aggtgcatgc gcacctggtg
     3181 gtgtgcgcac aatcggccgc cgccaacatc accaagtgcg tgcagaagtg cggcctgcag
     3241 gtcgacgacc tggtgctgtc gtcgctggcg tcgagcaccg cggtgctgac cccggacgag
     3301 cgcgaactcg gcgtggtgct ggtcgacatg ggcgcgggca ccaccgacat cgcggtgttc
     3361 gtgcagggcg cgatc
//
LOCUS       DAOOCX010000210         3298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_28096_length_3298_cov_4.370028, whole genome shotgun sequence.
ACCESSION   DAOOCX010000210 DAOOCX010000000
VERSION     DAOOCX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28096_length_3298_cov_4.370028"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2017)
                     /locus_tag="PK399_12540"
     CDS             complement(<1..2017)
                     /locus_tag="PK399_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPW13990.1"
                     /translation="MTRPTASLLPAGLLFACMAMAAPTFAADAPEVPRLVPRAASVQA
                     MPGRLVLAGNTHFVARDPRAKQVLAQLVRLAGDALPDTALRSANAGATLELKIVAGLD
                     VPAEGYRLQIAPDGGLLQAADADGLFNGAMTLLQLLRKDAAGTAWLPAVRIDDAPRFA
                     WRGLMLDSARHFQSVDEIKRLLDAMAELKLDTFHWHLTDDQGWRFPVPGWPKLTEVGN
                     CRIPAGDGGIDTMTGKPVPYCGVYTEAQIRDVVAHAASLHIEVVPEVDIPGHATAAIA
                     AYPQLGVSGQPIAVSNEWGVNVNLFNADETTMRFLEDVLGQLVKLFPGRYVHIGGDEA
                     VKDQWKESAKMQARIRELGVKDEAGLQAWMVARLERFLSTHDRRLLGWDEILDGELPP
                     SATVMSWRGTDGGIKAAKRGHDVVMAPWGKLYLDYLQTMSPDEEPGRPTQVTLETVYA
                     FEPVPAVLDATQAKHILGVQANVWTEHMRSFKRVQHAVFPRIAAVAESAWSPREGKDY
                     ADFLQRLPAMLPRWNALGITPAPTPFQPLIDASAGAGGKVRVEMSQPLGYAIRYTTDG
                     SAPNATSPTYAGPLSLAMPVDVQAQAFAGEQPLAPPVRRAFTASSLLTRSDEALAMCT
                     GQLMLRLEDDGPREGDRAIFNVDIFNPCWSWKQAPLAGIGAVEVRAGR"
     gene            complement(2128..>3298)
                     /locus_tag="PK399_12545"
     CDS             complement(2128..>3298)
                     /locus_tag="PK399_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW13991.1"
                     /translation="FPQGYTNYPGDYGTGIGGNFPRDVWYYTAEQMAAWNAAQANRDP
                     VGRRYPFYDFSLQEKTRAAFVQANLEGERWSGNVGVRLVKTSEDVFQYVPGGTATTPG
                     AITGSAFGNFSPFLISNDYTDILPSANFKFDVTDDLVLRLAATKTMARPDYSALAGGL
                     NLSPPASENDVGGGNSSNPNLKPIRSTNFDAALEWYYAPRALLSASVYYMDLASYVSR
                     GITEQSFLTFDQAHPNGYMARYLVSSPINAKASVKGAEFAVEQPIGEHFGVSANYSYT
                     DAKEDSGAPMVGASKNVFNLIGYFENDKLNARIAYNYRSHFFNGIDRLSAFNQDDTRS
                     VSASVGWTFNQHFAVTLDGMNLTGEKLKYYAENKDRPRAIYNNGRQFYVTLRAKF"
BASE COUNT          502 a         1086 c         1153 g          557 t
ORIGIN      
        1 tgcgcccggc ccgcacctcg accgcgccga tcccggccag cggcgcctgc ttccagctcc
       61 agcacggatt gaagatgtcg acgttgaaga tcgcgcggtc gccctcgcgc gggccgtcgt
      121 cttccagccg caacatcagc tggccggtgc acatcgccag tgcttcgtcg ctgcgcgtga
      181 gcagcgagga cgcagtgaac gcgcgacgca ccggcggcgc cagcggctgt tctcccgcga
      241 acgcctgcgc ctgcacatcg accggcatcg caagcgacag cggcccggcg taggtcggcg
      301 atgttgcatt cggcgcgctg ccatcagtgg tgtagcggat cgcgtagccg agcggctgcg
      361 acatctcgac acggaccttg ccgccggccc ctgcgctggc atcgatcagc ggctggaatg
      421 gcgtgggcgc cggggtgatg ccgagggcgt tccagcgcgg cagcatcgct ggcaggcgct
      481 gcaggaaatc ggcgtagtcc ttgccctcgc gcggcgacca tgcgctttcc gcgaccgcgg
      541 cgatgcgcgg gaacaccgcg tgctgcaccc gcttgaagct gcgcatgtgc tcggtccaca
      601 cgttcgcctg cacgccgagg atgtgcttcg cctgggtggc gtccagcacc gccggcaccg
      661 gctcgaaggc gtagaccgtc tccagcgtga cctgggtcgg tcggcccggt tcctcgtccg
      721 gcgacatggt ctgcaggtag tcgaggtaga gcttgcccca cggcgccatc accacgtcat
      781 ggccgcgctt ggccgctttg atcccgccgt cggtgccgcg ccaggacatc accgtcgccg
      841 acggcggcag ttcgccatcg aggatctcgt cccagccgag cagccgacgg tcgtgggtcg
      901 acaggaagcg ttccagccgc gccaccatcc aggcttgcag gccggcctcg tccttcaccc
      961 ccagttcgcg gatccgcgcc tgcatcttcg cggactcttt ccactggtcc ttgaccgcct
     1021 cgtcgccacc gatgtgcacg tagcggccgg ggaacagctt gaccagctgg ccgagcacgt
     1081 cctcgaggaa gcgcatggtg gtctcgtcgg cgttgaacag gttgacgttg acgccccact
     1141 cattggacac cgcgatcggc tggccggaca cgccaagttg cggatacgca gcaatcgccg
     1201 cggtggcatg gccggggatg tcgacttccg gcaccacctc gatgtgcagc gacgccgcat
     1261 gcgcgaccac gtcgcggatc tgcgcttcgg tgtagacgcc gcaatacggc accggcttgc
     1321 cggtcatggt gtcgatgccg ccgtcgccgg cgggaatgcg gcagttgccg acctcggtga
     1381 gcttcggcca gcccggcacc gggaaacgcc agccctggtc gtcggtgagg tgccagtgga
     1441 aagtgtccag cttgagttcg gccatcgcgt cgagcaggcg cttgatctcg tccaccgact
     1501 ggaaatggcg ggccgaatcc agcatcaacc cgcgccaggc gaagcgcggc gcgtcgtcga
     1561 tgcgcaccgc cggcagccac gcggtgccag cggcgtcctt gcgcagcaac tgcagcaggg
     1621 tcatcgcgcc attgaacagg ccgtccgcgt cggcggcctg caacaacccg ccatcgggcg
     1681 cgatctgcag gcggtagcct tctgccggca catcaagccc ggccacgatc ttcagttcca
     1741 gggtcgcgcc ggcattggcg gaacgcagcg cggtgtccgg cagggcatcg cccgccagcc
     1801 gcacgagttg cgccaggacc tgtttcgcgc gcgggtcgcg cgcgacgaaa tgggtgttgc
     1861 cggccagcac caatcgcccc ggcatggcct gcacggacgc ggcgcgcggc accagccgcg
     1921 gcacctcggg tgcatcggcg gcaaacgtcg gtgccgccat cgccatgcac gcgaacagca
     1981 ggccggcagg caacaaggag gcggtgggtc gggtcatggc gtcgatcctc gtctggcgtt
     2041 gggccccgcc ggccagcatg ttggcaaaaa agaggggccc ggagtgcggg ccccgatcgg
     2101 ggggtattgc agtgcgttgc cgcgcgatca gaacttcgcg cgcagggtga cgtagaactg
     2161 gcggccgttg ttgtagatcg cgcgcgggcg atccttgttc tcggcgtagt acttcagctt
     2221 ctcgccggtc aggttcatgc cgtccagggt gaccgcgaag tgctggttga acgtccagcc
     2281 caccgacgcc gacaccgagc gggtgtcgtc ctggttgaac gcggacaggc gatcgatgcc
     2341 gttgaagaaa tgcgagcggt agttgtaggc gatgcgcgcg ttgagcttgt cgttctcgaa
     2401 atagccgatc aggttgaaca cgttcttgga ggcgccgacc atcggggcgc cgctgtcttc
     2461 cttggcgtcg gtgtacgaat agttggcgct gacgccgaag tgctcgccga tgggctgctc
     2521 gaccgcgaac tccgcgccct tgaccgaggc cttggcgttg atcggcgagc tgaccaggta
     2581 acgggccatg tagccgttcg gatgcgcctg gtcgaaggtc aggaagctct gctcggtgat
     2641 gccgcggctg acgtagctcg ccaggtccat gtaatagacg ctggccgaca gcagcgcgcg
     2701 cggggcgtag taccactcca gcgcggcgtc gaagttggtc gagcggatcg gcttgaggtt
     2761 cgggttgctg gaattgccgc cgcccacgtc gttctcgctg gcgggaggcg acaggttcag
     2821 gccgccggcc agcgccgagt agtcggggcg ggccatggtc ttggtggcgg ccaggcgcag
     2881 caccaggtca tcggtgacgt cgaacttgaa gttcgcgctg ggcaggatgt cggtgtagtc
     2941 gttgctgatc aggaagggcg agaaattgcc gaacgccgaa ccggtgatcg cacccggggt
     3001 ggtcgcggtg ccgcccggca cgtactggaa gacgtcctcg ctggtcttga ccaggcgcac
     3061 gcccacgttg ccgctccagc gctcgccttc caggttggcc tggacgaagg cggccctggt
     3121 cttttcctgc agcgagaagt cgtagaacgg gtagcggcga ccgaccggat cgcggttggc
     3181 ctgcgccgcg ttccacgccg ccatctgctc ggcggtgtag taccagacgt cgcgcgggaa
     3241 attgccgccg atgccggtgc cgtagtcgcc cgggtaattg gtgtagccct gcgggaac
//
LOCUS       DAOOCX010000211         3283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_28263_length_3283_cov_7.422553, whole genome shotgun sequence.
ACCESSION   DAOOCX010000211 DAOOCX010000000
VERSION     DAOOCX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28263_length_3283_cov_7.422553"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1889)
                     /gene="dxs"
                     /locus_tag="PK399_12550"
                     /pseudo
     CDS             complement(1..1889)
                     /gene="dxs"
                     /locus_tag="PK399_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037575.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
     gene            2056..3279
                     /locus_tag="PK399_12555"
     CDS             2056..3279
                     /locus_tag="PK399_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HPW13992.1"
                     /translation="MADGDCIAQLGGWVGYRVEAWWHEQRGERRWLAVKLRPAPGEVR
                     RCSGCGEVVAAIHDRTLRRIRDLPVFGAPVELHVERLRLACPRCGPRLERLEWLDPHC
                     RVTRRLADSVARLCAVTSVLHAARWHGIDWKTAKAIDFRALERRLGPVDLDGVRRLAM
                     DEFAIQKGHRYATVVVDAERKRVLWVGRGRSRAEVRPFFELLGPARCARIEAVAMDMN
                     TAYDLEVRQHCPNARVVYDLFHVIAKYGREVIGRVRVDAANRLRHDKPARKVVKRAHW
                     ILLRNPEQLKEAEQVRLDEVLAANQSLMAVYVMKEQLKALWTVPTAWAWRRAWTQWLR
                     HARESGIPALMHFAKCLRPYWRGIVSRVRWPMHTGLVEGINNRIKVIKRIAYGFRDDA
                     YFFLKIRAAFPGVGR"
BASE COUNT          506 a         1068 c         1153 g          556 t
ORIGIN      
        1 ctgcgccggc accgtcgcgt gtggccagcg cttgagcacc gcattgcgga tgccggccac
       61 gtccagtccg gcttcggcga gcaggtcttc gcggctggcg tggtgctgga aggcatcggg
      121 caggcccagg tgctggatcg gcaggacgat gccttcggcg gccagcagct cggccacgcc
      181 gctgccggcg ccaccggcga ccacgttgtc ctccagggtc acgaaccctt cgtgcgagtt
      241 cgcgagttcg aggatcagcg cgcgatccag cggcttcacg aagcgcatgt tgaccacggt
      301 caggccaagc tccgcgccga ccgtttccgc tgccggcacc aatgcgccga aggcgagcaa
      361 cgcgatacgc gaaccggtgc gacgacgctc cgccttgccg atcggcaggg tctcgagcga
      421 cgcgtccagc gccacgcccg gaccggtgcc gcgcgggtag cgcaccgcgg ccgggccttc
      481 gtggcggtag ccggtgctga gcatgcgccg gcattcgagt tcgtccgccg gggccatcac
      541 gaccatgttc ggcacgcagc gcaggaagct gaggtcgagg ttgccggcgt gggtcgcacc
      601 gtccgggccg accacgccgc cgcggtcgat cgcgaacagc acgtcgagct tctggatggc
      661 gacgtcgtgg accagctggt cgtaggcgcg ctgcaggaag gtggagtaga tcgcgaccac
      721 cggcttggcg ccttcgcagg ccatgccggc ggcgagggtg accgcgtgct gctcggcgat
      781 ggcgacgtcg aaataacgcg ccgggtattc ctggctgaag cgcacgaggc cggagccttc
      841 gcgcatcgcc ggggtgatcg ccagcagcgt gtcgtccgcg gcggccatgt cgcacagcca
      901 atcgccgaag acatcggtgt acgtcggctt cttcgcgccg gtcttcgcca ccaagccctt
      961 ctcgggtcga acggcgagac cgcgtggtag ccgatctggt cgccttcggc cagctcgtag
     1021 cccttgccct tggtggtgat gacatgcagc agctgcggac ccttcaggcc cttgagcatg
     1081 gtcagcacgt cgtgcagcgc gtcgacgtcg tggccgtcga tcgggccggt gtagtggaaa
     1141 cccatttcct cgaacagggt ggagggcacg aacatgccct tccagtgttc ttcccagcgc
     1201 cgcacgaagc gcgcggtgcc gctgctgcgc ttgtcgccga ggagcttctt gccgccctcg
     1261 cgcagcgcgt tgagggtctt gctgccggtc atccgaccga gcatcttggt gagtccgccg
     1321 accgcctcgc tgatcgacat gcggttgtcg ttgaggatca ccagcaggtt gggttcctcg
     1381 tccatgccgc cggcgtggtt gagcgcctca tagaccatgc ccgcggtcat cgctccgtcg
     1441 ccgatgaccg cgacaacctt gcggtcgttg cctgcgcggg cattggcgat ggccatgccc
     1501 agtgctgccg agatcgaggt cgacgaatgg cccacgccga acgtgtcgta ttcgctctcc
     1561 tcgcgcttcg ggaacggcga cacgccatcc ttctgcttga ccgtgtggat ggtgtcgcgg
     1621 cgaccggtca ggatcttgtg cggatagctc tggtggccga cgtcccagac gatgcggtcg
     1681 tcgggggtgt cgtagagata gtgcagcagg gtggtcagtt cgaccacgcc caggccagcc
     1741 ccgaaatggc ctcccgactt gcccaccgac tcgatcaggt agccgcgcaa ctcatccgcg
     1801 atggcgcgca gttcggaggg ctcgaaggcg cgcaggtcgg ccgggcggtc gatccggaac
     1861 agccggggat aacgggtggc atcgatcatg tcgctattgt ccccctgccc gcgggaaggg
     1921 gcaatgaagc ggcctgaaca aggcgggcgc gccccgatcc ccccaaaaaa aggggttctt
     1981 ctcccatctg agggagaggc gcacggccga cccggttagt ttgcagttgt cgagtcaaca
     2041 aaccgggggt cggccgtggc cgacggggat tgtattgccc agctgggagg ctgggtgggg
     2101 tatcgggttg aggcgtggtg gcacgagcag cgcggcgagc gtcgttggct ggcagtgaag
     2161 ctgaggccag cgcctggtga agttcgtcgc tgcagcggct gcggcgaggt cgttgcggcc
     2221 atccacgacc gcacgctgcg gcggatacgc gacttgccgg tgttcggggc cccggtcgag
     2281 ctgcacgtcg agcgcctgcg actggcttgt ccgcgttgcg ggccgcggct ggagcgcctt
     2341 gagtggctgg atccccattg ccgggtgacc cggcgcttgg ccgacagcgt cgcccgcctg
     2401 tgcgcggtga cctcggtgct gcatgccgcc cgctggcatg gcatcgactg gaagaccgcc
     2461 aaggccatcg acttccgggc tttggagcgt cgcctgggtc cggtggatct ggacggcgta
     2521 cgccggctcg cgatggacga gttcgcgatc caaaagggcc accgctacgc caccgtggtg
     2581 gtcgacgcag agcgcaagcg ggtgctgtgg gtgggacgtg gccgctcccg cgccgaggta
     2641 cggccgttct tcgagctgct cgggcctgcg cgctgcgcac gcatcgaggc cgtggccatg
     2701 gacatgaaca cggcctacga cctggaggtg cgccagcact gcccgaacgc ccgggtggtc
     2761 tacgacctgt tccatgtcat cgccaagtac ggcagggaag tgatcggccg cgtgcgcgtg
     2821 gatgctgcca accgattgcg tcatgacaag ccggcccgca aggtcgtcaa gcgcgcgcac
     2881 tggatcctgc tgcgcaaccc ggagcagctc aaggaagccg agcaggtgcg actggacgag
     2941 gtactggccg ccaaccagtc gctgatggcg gtctacgtaa tgaaggagca gctcaaggca
     3001 ctatggacgg taccgaccgc ctgggcctgg aggcgcgcct ggacgcagtg gctgcgtcac
     3061 gcccgggaaa gtggcatccc ggccctgatg cactttgcca agtgcctgcg cccctactgg
     3121 cgcggcatcg tcagccgggt gcgctggccg atgcacaccg gcctcgtcga aggcatcaac
     3181 aaccgcatca aggtcatcaa gcgcatcgcc tacggcttcc gcgacgacgc ctacttcttc
     3241 ctcaagatcc gagccgcatt ccccggagtt gggagatgaa cct
//
LOCUS       DAOOCX010000212         3242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_28829_length_3242_cov_5.524318, whole genome shotgun sequence.
ACCESSION   DAOOCX010000212 DAOOCX010000000
VERSION     DAOOCX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28829_length_3242_cov_5.524318"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PK399_12560"
     CDS             <1..1154
                     /locus_tag="PK399_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HPW13993.1"
                     /translation="TVTPLRPLAVAAAAYSRAKPFAAPVLANQRITFGAGGKDVRHLE
                     LALDGAGLAYEPGDALGLWPRNPEPLVDAVLQATALDGETAVRIGEEERSLRAWLLDK
                     RELTRLARPFVAAQAERSGNADLQRLLAPEHAGDFQQLLASLQPIDLLQRQRADWDAE
                     SLVAALRPLQPRLYSIASSRKLVGDEAHLTVAHVEYDIEGQARWGAASHQLATRAEGD
                     TLPVYIEANERFRLPADASRDIIMIGAGTGVAPFRGFVQERAALGGTGRNWLFFGAPH
                     QRNDFLYQLEWQRALKDGQLQRIDLAFSRDTARKVYVQQRITEQARALHDWLQGGAHL
                     YVCGATAMGKDVHAALVDALAAHNGGDRDAATDALDTLAREGRYARDLY"
     gene            1154..2887
                     /gene="cysI"
                     /locus_tag="PK399_12565"
     CDS             1154..2887
                     /gene="cysI"
                     /locus_tag="PK399_12565"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011409143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HPW13994.1"
                     /translation="MSAHSVEDIKRASAGLRGTLLASLADAVTGALRDDDQTLIKYHG
                     SYQQDDRDVREARRLAKLEPAFSFMIRTRTPGGVINPRQWLQLDAIASTYAERGLRIT
                     TRQAFQFHGVVKGELKATMRAINAALIDTLAACGDVNRNVAVAANPLHPGVQAQVLAQ
                     AVAVSKHLLPNTRAYYELWLDEERVGGSGEEVEPIYGQAYLPRKFKIGFAIPPLNDVD
                     VFAQDLGFIAILDDAGALLGYNVSIGGGMGATHGDPETYPRLGNVIGFATPDQVIAVA
                     ESVVTAQRDFGNRAVRKRARLKYTIDDRGLDWFKDEVERRAGFPLGPARAFRFDHNGD
                     RFGWVDAGHDLQHVTLRIPSGRIVDGALDGRGDAVLHLTGLREIARLLQAEGGNAEFR
                     LTSNQNLVIANIPATLQSRVDALLREHALDGWRSASPLRLNALACVALPTCGLAMAEA
                     ERYLPDLVNEVDALLARHGIADAPIHLRISGCPNGCSRPYLGEIALVGKAPGRYNLML
                     GADHRGERLNTLYRENIDEPQILAALDPLFAAYASERAQDERFGDWLLRNGTVAAPAP
                     RHRTIPIEVAA"
     gene            2884..>3242
                     /locus_tag="PK399_12570"
     CDS             2884..>3242
                     /locus_tag="PK399_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HPW13995.1"
                     /translation="MSPPDPISAAESPHALSALNRWLARLSAQERVAWAMDSLAAPHA
                     LSTSFGTQSAVSLHLLTQAKPDIPVILVDTGYLFPETYRYADQLVEQLGLNLKVYRPQ
                     IGIAWMEARFGKLWEGG"
BASE COUNT          531 a         1185 c         1052 g          474 t
ORIGIN      
        1 ccaccgtcac cccgctccgt ccgcttgccg tggcggccgc agcatattca cgcgccaagc
       61 cgttcgccgc cccggtgctc gccaaccaac gcatcacttt cggtgccggc ggcaaggacg
      121 tgcgccatct cgagcttgcc ctcgacggtg ccggcctggc ctacgaaccc ggcgatgcgc
      181 tgggcctgtg gccgcgcaac cccgagccgc tggtcgatgc cgtgttgcag gccactgcat
      241 tggacggcga gaccgcagtg cgcatcggcg aggaagaacg cagcctgcgc gcgtggttgc
      301 tcgacaagcg cgagctcacc cgcctcgccc gcccgttcgt ggccgcgcag gccgagcgca
      361 gtggcaatgc cgacctgcag cgcctgcttg cgcccgaaca tgccggtgac ttccagcaac
      421 tgctggccag cctgcagccc atcgacctgc tgcaacgcca gcgcgcggat tgggatgccg
      481 agtcgctggt cgccgcgcta cgcccgttgc aaccgcgcct gtattccatc gcctccagcc
      541 gcaagctcgt cggcgacgaa gcgcatctca ccgtcgcgca cgtcgaatac gacatcgagg
      601 gccaggcgcg ctggggtgcg gcctcgcacc aactggcaac ccgtgccgaa ggcgacaccc
      661 tgccggtcta catcgaagca aacgagcgct tccgcctgcc agccgatgcc agccgcgaca
      721 tcatcatgat cggtgccggc accggcgtgg cgccgttccg cggcttcgtg caggaacgcg
      781 cggcgctggg cggaactggc cgcaactggc tgttcttcgg cgcaccgcat cagcgcaacg
      841 atttcctcta ccaactggaa tggcagcgcg ccttgaagga cggccagttg cagcgcatcg
      901 acctggcgtt ctcgcgcgac accgcacgca aggtctatgt gcagcagcgc atcaccgaac
      961 aggcgcgcgc cctgcacgac tggctgcagg gcggcgcgca cctctacgtc tgcggcgcga
     1021 ccgcgatggg caaggacgtg catgcggcgc tggtcgatgc gctggccgcg cacaacggcg
     1081 gcgaccgcga cgcggcgacg gacgctctcg acacattggc ccgtgagggc cgctacgcgc
     1141 gggacctgta ctgatgagcg cgcattcggt cgaggacatc aagcgggcca gcgcgggcct
     1201 gcgcgggacc ctgctcgcct cgctggcgga cgcggtcacc ggcgccctgc gcgatgacga
     1261 ccagaccctg atcaagtacc acggcagcta ccagcaggac gaccgcgacg tgcgcgaagc
     1321 gcgccgcctc gccaagctgg aaccggcctt cagtttcatg atccgcaccc gcacgccggg
     1381 cggcgtgatc aaccccaggc agtggctgca actggatgcg atcgccagca cctatgcgga
     1441 acgcggcctg cgcatcacca cccgccaggc cttccagttc cacggcgtgg tcaagggcga
     1501 actcaaggcg acgatgcggg cgatcaacgc cgcattgatc gacacactgg ccgcctgcgg
     1561 cgacgtcaat cgcaatgtcg ccgtcgctgc caacccgctg catccgggcg tgcaggcaca
     1621 ggtgctggcg caggccgtgg cggtgtcgaa gcacctgctg ccgaacaccc gcgcctacta
     1681 cgagttgtgg ctggacgagg aacgcgtggg cggcagcggc gaggaagtcg aaccgatcta
     1741 cggccaggcc tacctgccgc gcaagttcaa gatcggtttc gcgatcccgc cgttgaacga
     1801 tgtcgacgtg ttcgcgcagg acctgggctt catcgcgatc ctcgacgacg ccggcgcgtt
     1861 gctgggctac aacgtcagca tcggcggcgg catgggcgcg acccacggcg atcccgagac
     1921 ctatccgcgg ctcggcaacg tcatcggctt cgccacgccc gaccaggtca tcgcggtcgc
     1981 ggagtccgta gtcaccgcgc agcgcgactt cggcaaccgc gcggtgcgca agcgcgcgcg
     2041 cctgaagtac acgatcgacg atcgcgggct ggactggttc aaggacgaag tggaacggcg
     2101 cgccggcttt cccctggggc cggcgcgtgc gttccgtttc gaccacaacg gcgaccgctt
     2161 cggctgggtc gatgccggcc acgacctgca gcacgtcacc ctgcgcatcc cgtcggggcg
     2221 catcgtcgat ggtgcgctgg acggtcgcgg cgacgccgtc ctgcacctca ccggcctgcg
     2281 cgagatcgcg cggctgctgc aggcagaagg cggcaacgcc gagttccgcc tgacctccaa
     2341 ccagaacctg gtgatcgcca acatcccggc cacgctgcaa tcgcgcgtgg atgcgctgct
     2401 gcgggaacat gcgctggatg gctggcgcag cgcctcgccg ttgcggctca acgcactggc
     2461 ctgcgtcgcc ctgccgacct gcggcctggc gatggccgaa gcggaacgct acctgcccga
     2521 cttggtcaac gaagtcgacg cactgctggc ccgccacggc atcgccgatg caccgatcca
     2581 cctgcgcatc agcggctgcc cgaacggctg ctcgcggccc tacctcggcg agatcgccct
     2641 ggtcggcaag gcgcccggcc gctacaacct gatgctcggc gccgaccatc gcggcgagcg
     2701 gctcaacacc ctgtatcgcg aaaacatcga cgagccgcag atcctcgccg cgctggatcc
     2761 gctgttcgcg gcctacgcca gcgagcgcgc gcaggacgaa cgtttcggcg actggttgct
     2821 gcgcaacggc accgtcgctg cccccgcacc acgtcatcgc accatcccca tcgaggttgc
     2881 cgcatgagcc cgcccgatcc catcagcgcc gccgaatcgc cgcacgcgct atccgcgctg
     2941 aatcgctggc tggcccgcct gtcggcgcag gagcgcgtcg cctgggcgat ggacagcctg
     3001 gccgccccgc acgcgctgtc gaccagcttc ggcacgcaat cggcggtgtc gctgcacctg
     3061 ctgacccaag ccaagccgga catcccggtg atcctggtcg ataccggcta tctcttcccg
     3121 gaaacctatc gttacgccga ccaattggtc gaacagctcg ggctgaacct caaggtgtac
     3181 cggccgcaga tcggcatcgc ctggatggaa gcgcgcttcg gcaagctgtg ggaaggcggc
     3241 ag
//
LOCUS       DAOOCX010000213         3237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_28892_length_3237_cov_11.334067, whole genome shotgun
            sequence.
ACCESSION   DAOOCX010000213 DAOOCX010000000
VERSION     DAOOCX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28892_length_3237_cov_11.334067"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PK399_12575"
     CDS             complement(<1..638)
                     /locus_tag="PK399_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPW13996.1"
                     /translation="MRVLRVLPIAVRMVAITITGIAAATATTVLLARWMPSWVAALVS
                     AVVIGSVVAAIIWRALVPMRALFRALAGTVASYRDGDFSFGITWDKASDLIELVDAHN
                     ALGNALREQRLALVQRELLLDTMVQNTPVAMVLVDAARRIVLGNLAARRMLGEGRRLE
                     GRAFDDVLANADTALQEAFERGGDGMFTVGSDDDEDIYHLSRRGFRLNGRPH"
     gene            complement(638..1978)
                     /locus_tag="PK399_12580"
     CDS             complement(638..1978)
                     /locus_tag="PK399_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016945136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPW13997.1"
                     /translation="MPTILVIDDNRAVSTALEVLFSLHDIDTLHAESPREGLDLLERE
                     QVDLVIQDMNFEADTTSGDEGTALFHQIRTRHPDLPVILLTAWTHLESAIDLVKAGAA
                     DYLAKPWDDRKLLVTVNNLLELAEARRELTQAQLGERRRRDALLRDYDLRGIVYADPA
                     SENALALACQVAKSDIPVLVTGPNGAGKERYAEIIHANSLVRNGPFIALNCGALPSEL
                     IEAELFGAEAGAYTGANKPREGKFEAADGGTLFLDEIGTLPLAGQVKLLRVLETGRFQ
                     RLGSNRERSVKVRIVSATNANLQALIADGRFREDLFYRLNGIELRLPPLAQRPRDILP
                     LARHFLPAGKRLGDDAERALQRHAWPGNVRELRNAVQRAALLARSDVIAAADLALPPG
                     ALDAGNTGGDEPDRASIEAALERNGGVLAQAAADLGLSRQALYRRLDRLGIARN"
     gene            complement(2000..2488)
                     /locus_tag="PK399_12585"
     CDS             complement(2000..2488)
                     /locus_tag="PK399_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW13998.1"
                     /translation="MNHAFARSGIALLAAAAMAASAATPEPPTLQATIAALDTAVFDA
                     FNHCADPAQLQAHAAHFAPDVEFYHDTGGVTWTRDAMIANTAKYACGHYTRELAAGSL
                     QVVPVKDFGAIAQGVHRFCRVDTGRCEGEADFTMVWRLRDGQWTITRVLSYGHRPSTA
                     TH"
     gene            complement(2533..>3237)
                     /locus_tag="PK399_12590"
     CDS             complement(2533..>3237)
                     /locus_tag="PK399_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPW13999.1"
                     /translation="SMAEKLFPGQNPLGKVFYSWGESPTAVIGVIEDLARPNSVGQGP
                     YAMIFALREYGPGLHFILRTDPGRRADVLKAAVTALKKTDPNTLVVNEDTFDEIRAGF
                     FEQDRSMAGMLIVVIAALMLVTALGIVGLASFWVQQRTKQIGVRRALGATRGQILRYF
                     QAENFLLASAGIVLGMLLAYGINQLLMSKYELPRLPLFYLPIGAVALWVLGQVAVYWP
                     ARRAASVPPAIATRSV"
BASE COUNT          505 a         1079 c         1138 g          515 t
ORIGIN      
        1 tcgtgcgggc ggccgttgag gcggaagccg cgtcgcgaca ggtggtagat gtcctcgtcg
       61 tcgtcgctgc ccacggtgaa catgccgtcg ccaccgcgtt cgaaggcttc ctgcagggcg
      121 gtgtcggcgt tggccagcac gtcgtcgaag gcgcgtcctt ccagccgccg gccttcgccg
      181 agcatccgtc gcgcggcgag gttgcccagc acgatgcggc gcgccgcatc caccagcacc
      241 atcgccaccg gggtgttctg caccatcgtg tcgagcagca actcgcgctg caccagcgcc
      301 agccgttgct cgcgcaaggc gttgccgagc gcgttgtgcg cgtcgaccag ttcgatcagg
      361 tcgctggcct tgtcccaggt gatgccgaaa ctgaaatcgc catcgcgata gctcgccacg
      421 gtgccggcca gcgcgcggaa cagcgcgcgc atcggcacca gcgcgcgcca gatgatcgcc
      481 gcgaccacgc tgccgatgac cacggcagag accaacgccg cgacccacga cggcatccag
      541 cgtgcgagca acacggtcgt cgcagtcgcg gccgcgatcc cggtgatcgt gatcgcgacc
      601 atccgcaccg cgatcgggag gacgcgcagg acgcgcatca gttgcgcgcg atgccgaggc
      661 ggtcgagccg gcgatacagc gcctgccgcg acaatcccaa atccgccgcg gcctgtgcca
      721 gcaccccgcc attgcgctcg agcgcggctt cgatgctggc gcggtcgggt tcgtcgccgc
      781 cggtgttgcc ggcatccagc gcgcccggag gcagggcgag gtccgcggcc gcgatgacat
      841 cgctgcgcgc gagcagggcc gcgcgctgca cggcattgcg caattcgcgc acgttgcccg
      901 gccaggcgtg gcgctgcaac gcgcgctcgg cgtcgtcgcc caggcgcttg cccgcgggca
      961 ggaagtggcg ggccagcggc aggatgtcgc gcgggcgctg cgccaagggc ggcaggcgca
     1021 gttcgatgcc gttgagccgg tagaacaggt cttcgcggaa gcgaccatcg gcgatcagcg
     1081 cctgcaaatt cgcattggtc gcgctgacga tgcgcacctt gacgctgcgt tcgcgattgc
     1141 tgcccagccg ctggaagcgg ccggtctcca ggacgcgcaa gagcttgacc tggccggcca
     1201 gcggcagggt gccgatttcg tcgaggaaca aggtgccacc atcggccgcc tcgaacttgc
     1261 cttcgcgcgg cttgttggcg ccggtgtagg caccggcctc ggcaccgaag agttcggctt
     1321 cgatcagctc gctgggcaac gcgccgcaat tgagtgcgat gaaggggccg ttccgtacca
     1381 gcgaattggc gtggatgatc tcggcgtaac gttccttgcc ggcaccattc gggccggtga
     1441 ccagcaccgg gatatcggac ttcgcgacct ggcaggccag cgccagcgcg ttctcgctgg
     1501 ccggatccgc atagacgatg ccgcgcaggt cgtagtcgcg cagcagcgca tcgcggcgac
     1561 ggcgctcgcc cagttgcgcc tgggtgagct cgcgacgcgc ctcggcgagt tccagcaggt
     1621 tgttcaccgt caccagcagc ttgcggtcgt cccagggctt ggccaggtaa tcggcggcac
     1681 cggccttgac caggtcgatc gccgattcca ggtgcgtcca cgccgtcagc aggatcaccg
     1741 gcaggtcggg atgtcgcgtg cggatctggt ggaacagcgc ggtgccttcg tcgccggagg
     1801 tggtgtcggc ctcgaagttc atgtcctgga tgaccaggtc gacctgctcg cgttccagca
     1861 ggtccaggcc ctcgcgcggc gactccgcat gcagcgtgtc gatgtcgtgc agcgagaaca
     1921 gcacctcgag cgcggtgctg accgcgcggt tgtcgtcgat gaccaggatg gtgggcatgg
     1981 gatccagtcg gccgtcgatt cagtgcgttg cggtgctggg ccggtggccg taactcagca
     2041 cgcgggtaat ggtccattgg ccgtcgcgta gccgccagac catggtgaag tcggcttcgc
     2101 cctcgcagcg gccggtgtcg acccggcaga accgatgcac gccctgggcg atggcgccga
     2161 agtccttgac cggaaccacc tgcaggctac cggcggccag ttcacgcgtg tagtgcccgc
     2221 aggcgtactt cgcggtgttg gcgatcatcg cgtcgcgcgt ccaggtgacg ccgccggtgt
     2281 catggtagaa ctcgacatcg ggcgcgaaat gcgcggcatg cgcctgcaac tgcgcgggat
     2341 ccgcgcagtg gttgaaggcg tcgaacaccg cggtgtcgag cgctgcaatg gtggcctgca
     2401 gcgtcggcgg ctccggcgtg gccgcggagg cagccatggc cgccgccgcg agcagcgcga
     2461 tgccggatcg tgcgaaggca tggttcatgg cgattccatc ggcatggcgc gattgtaggc
     2521 ggaggtcatc gatcagaccg aacgcgtggc gatggccggc ggcaccgatg ccgcacgccg
     2581 cgccggccag tacaccgcga cctgcccgag tacccacagc gcgacggcac cgatcggcag
     2641 gtagaacagc ggcagtcgcg gcagttcgta cttgctcatc agcaactggt tgatgccgta
     2701 cgccagcagc atgcccagga cgatgccggc gctggccagc aggaaattct ccgcctggaa
     2761 gtagcgcagg atctgcccgc gagtcgcccc cagcgcgcgg cgcacgccga tctgcttggt
     2821 gcgctgctgc acccagaaac tggccaggcc gacgatgccg agtgcggtca ccagcatcag
     2881 cgcggcgatc accacgatca gcatccccgc catcgagcgg tcctgctcga agaagccggc
     2941 gcggatctcg tcgaaggtgt cttcgttgac caccagcgtg ttgggatcgg tcttcttgag
     3001 cgcagtaacc gcggccttga gcacgtccgc gcgacggccg ggatcggtgc ggaggatgaa
     3061 gtgcaggccg gggccgtatt cgcgcagcgc gaagatcatc gcgtagggac cctggccgac
     3121 gctgttgggg cgtgccaggt cctcgatgac gccgatcacc gcggtcggcg attcgcccca
     3181 gctgtagaag accttgccca gcgggttctg gcccgggaac agtttttcgg ccatcga
//
LOCUS       DAOOCX010000214         3233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_28952_length_3233_cov_7.558527, whole genome shotgun sequence.
ACCESSION   DAOOCX010000214 DAOOCX010000000
VERSION     DAOOCX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28952_length_3233_cov_7.558527"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PK399_12595"
     CDS             <1..229
                     /locus_tag="PK399_12595"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HPW14000.1"
                     /translation="TGLADTAPALDVLELNELLSSLAQADARAAEGVELRCFGGYTEP
                     EIAHIQGITERTVQRDWRRARAFLMAQLGSA"
     gene            226..3195
                     /locus_tag="PK399_12600"
     CDS             226..3195
                     /locus_tag="PK399_12600"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPW14001.1"
                     /translation="MSIDPARLQRTLQLFDAALDQPAATRAAWLAGECGGDDDLLRDV
                     QAMLAADAGDDADPITPKLASLREDSTDPVELPAGTRIGPWNVREVLGRGGMGAVYRV
                     ERVDGAYRHEAALKRIRIGLDSALARQRFLRERQILARLQHPHIAGLLDGGVDDHNAP
                     WFVMPLVEGERIDRWCDARTLDVRGRVRLLLQVLDAVAFAHRQLVVHRDLKPSNIVVD
                     ASGHAFLLDFGIAKLLEDDDAGSTSTGERAFTPEYASPEQLHGEPVSTATDLYQLGVL
                     LYALLAQAHPYGLTGETPLRTRLTRMDGDPQALWDAALHSCAEDAAKRGSTPAALAKQ
                     LRGDLSAIAQRCLVSDATQRYGSVDALRADLLAWLDGKPVSARAPTLRYRASRFIRRN
                     ALAVGALSAVVVALCGGLGSALWQAREARLQAQRADAVNNYLVDVFRSIDPEEGFGPD
                     ATARQLIDLGVARLDRDELRGQPGAEGKLRLTLADSYIALGEYTKADAELARALVLLP
                     ASQADDVLQVRFRQAELRLEADDVDGLQRKLDESDAWLRAHASSLREPEVVAAQLQME
                     RAKLQDHLEDYERAVPLAREAWQRMRKLRGDDDEDTRSMLGEYAAILSNNGEFDTAAA
                     LAKDVAEGIRRARPTNQFALATALHNRAVYLSRGGKMEPAIATEQQALAIRRRILPAD
                     HPYIAASLGELATMIGTTSRAGEALPLYEQAVAIMRKQPPGSEYELGLRINNWGVGCY
                     EQGDMACSMDKLREAVGIWDKVLPADHSNLLTARSNLASLYSRGGALQQGEAQLRQVV
                     AALEASNAAKPSADSAKALSSARSELTTNLLNQGRAADALALARRRWKELQATRNADE
                     TAANALSTLALAELANDLPQPALAHARDALRRDHVDAPGSRREGFALMTLARAQLASG
                     DATAATSHARQAVAMFTTALSADHLNTGLARGVLGRALLASNQRSEARKELDAAIAIL
                     SEKAAWLPELSELKRLR"
BASE COUNT          545 a         1130 c         1097 g          461 t
ORIGIN      
        1 caccggcctg gccgacaccg cgcccgcgct ggacgtgctg gaactcaacg aactgctctc
       61 gtcgctggcg caggccgacg cgcgcgcggc cgagggcgtg gaactgcgct gcttcggcgg
      121 ctacaccgaa ccggagatcg cccacatcca gggcatcacc gaacgcaccg tgcaacgcga
      181 ctggcggcgc gcgcgcgcgt tcctgatggc gcaactgggc agcgcatgag catcgacccg
      241 gcgcgcctgc agcgcaccct gcagttgttc gatgccgccc tcgatcaacc tgcagccaca
      301 cgcgcggcct ggcttgcagg cgaatgcggc ggcgatgacg acctgcttcg cgacgtgcag
      361 gcgatgctgg ccgccgatgc cggcgacgac gcggatccga tcaccccgaa gctggcgtcg
      421 ctgcgcgagg acagcaccga tccggtggaa ctgccggccg gcacgcggat cggcccatgg
      481 aacgtccggg aggtgctcgg ccggggcggc atgggcgcgg tgtatcgggt cgaacgcgtc
      541 gatggtgcct accgccacga agcggcgctg aagcgcatcc gcattggcct cgacagcgcg
      601 ctcgcgcgac agcgtttcct gcgcgagcgg cagatcctgg cccgcctgca gcatccgcac
      661 atcgccggcc tgctcgatgg cggcgtcgac gaccacaacg cgccctggtt cgtgatgcca
      721 ttggtcgagg gcgaacgcat cgaccgctgg tgcgatgcgc gcacgctcga cgtgcgtggc
      781 cgcgtgcgcc tcctgctgca ggtgcttgat gcggtggcgt tcgcgcatcg gcagctggtg
      841 gtgcatcgcg acctgaagcc atcgaacatc gtggtcgatg ccagtggcca tgcgttcctg
      901 ctggacttcg gcatcgccaa gctgctggag gacgacgacg ccggcagcac cagcaccggc
      961 gagcgtgcat tcactcccga atacgcatcg cccgaacagt tgcatggcga gccggtgtcg
     1021 accgccaccg atctctacca acttggcgtg ctgctgtacg cgctgctggc gcaggcgcat
     1081 ccctatggcc tgaccggcga aacgccgctg cgcacgcgac tgacgcggat ggacggagat
     1141 ccgcaggcgc tgtgggatgc cgcgctgcat tcctgcgccg aagacgccgc gaagcgcggg
     1201 tcgacccccg ccgcgctggc gaaacaattg cgcggcgacc tgtccgcgat cgcgcaacgc
     1261 tgcctggtct ccgatgccac gcagcgctat ggcagcgtgg atgcgctgcg cgcggacctg
     1321 ctggcgtggc tggacggcaa gccggtctcc gcccgcgcgc cgaccctccg ttaccgcgcc
     1381 agtcgtttca tccgccgcaa tgcactggcg gtcggtgccc tctccgcggt ggtggtggcg
     1441 ctgtgtggtg gcctcggttc cgccttgtgg caggcacgcg aggcccgcct gcaggcgcag
     1501 cgcgcggatg ccgtgaacaa ctatcttgtc gatgtcttcc gcagcatcga cccagaagaa
     1561 ggctttgggc ctgacgccac cgcgcggcaa ctcatcgacc tcggcgtcgc gcgcctggat
     1621 cgcgacgaac tgcgcggcca gcccggggcg gaaggcaagc tgcggctgac cctggccgac
     1681 agctacatcg cactgggcga gtacacgaag gcggatgcgg aactcgcgcg cgccctcgtc
     1741 ctgctgccgg cttcgcaggc cgacgacgtg ctgcaggtac gcttccgcca ggccgaattg
     1801 cgactggaag ccgacgacgt ggacggcctg cagcgcaagc tcgatgaaag cgatgcatgg
     1861 ctgcgcgctc acgcatcgtc cctgcgcgaa cccgaggtag tcgcggccca gttgcagatg
     1921 gaacgcgcga agctgcagga tcacctcgag gattacgaac gtgccgtgcc gcttgcgcgc
     1981 gaggcctggc aacgcatgcg caagttgcgt ggcgacgacg acgaggacac ccgctcgatg
     2041 ctgggcgaat acgccgccat cctgagcaac aacggcgagt tcgacaccgc cgcggccctc
     2101 gccaaggacg tcgcggaggg tatccgccgc gccagaccga ccaaccaatt cgcgcttgcg
     2161 accgcactgc acaaccgggc ggtctacctg agccggggcg gcaagatgga accggcgatc
     2221 gcgacggagc agcaggcctt ggcgatccgt cgccgcatcc tgccggccga ccatccctac
     2281 atcgcggcga gtctgggcga actggcgacg atgattggaa ccaccagccg cgccggcgag
     2341 gcgttgccgc tctacgaaca ggcggtggcg atcatgcgca agcagccgcc cggcagcgaa
     2401 tacgaactgg gcttgcggat caacaactgg ggcgtcggct gctatgaaca gggcgacatg
     2461 gcctgctcga tggacaagct ccgcgaggcg gtcggcatct gggacaaggt cctgcccgcc
     2521 gaccacagca acctgctgac cgcgcgcagc aacctggcct cgctgtacag ccgcggcggc
     2581 gcactgcaac agggcgaagc gcaattgcgc caggtcgtgg ctgcactgga agcgtccaac
     2641 gccgccaagc cgagcgcgga cagcgccaaa gcgctgtcca gtgcgcgcag cgaactgacc
     2701 accaacctgc tcaaccaagg ccgcgccgcc gatgcgctgg cgctggcgcg ccgacgctgg
     2761 aaagaattgc aggccacccg caatgcggac gagaccgccg cgaatgcttt gtccacgcta
     2821 gcgctggccg aactcgccaa tgacctcccg caaccggccc tggcgcatgc acgcgacgca
     2881 cttcgacgcg accatgtgga tgcaccaggc agccgccggg aaggcttcgc gctgatgacg
     2941 ctggcgcgcg cgcaactggc gtccggggat gcgactgctg cgacttcgca cgcacgccag
     3001 gccgtggcga tgttcaccac cgcgctcagt gccgaccatc tcaatacggg cctggctcgt
     3061 ggggtgctgg gtcgcgccct gctcgcctcc aaccagcgca gcgaagcccg caaggaactc
     3121 gatgccgcca tcgccatcct gtcggaaaag gcggcgtggc tgccggagtt gtccgagctg
     3181 aaacggctgc gctgagcgcg tccggattgg cctgtgcgaa actccgacgc gac
//
LOCUS       DAOOCX010000215         3209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_29273_length_3209_cov_5.534559, whole genome shotgun sequence.
ACCESSION   DAOOCX010000215 DAOOCX010000000
VERSION     DAOOCX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29273_length_3209_cov_5.534559"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /gene="ilvB"
                     /locus_tag="PK399_12605"
     CDS             complement(<1..1426)
                     /gene="ilvB"
                     /locus_tag="PK399_12605"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPW14002.1"
                     /translation="MNGARWLAQALVAEGVETLFGYPGGTIMPFYDALHSAPALKHVL
                     VRHEQGAAFAANGYARASGRVGVCVATSGPGASNLVTGIADAMLDSVPMVIITGQVPT
                     HLMGTDAFQELDVFGMTLPMVKHSFIARRVEDLPMMVAEAFRLARSGRPGPVLIDLPK
                     DVQMADASHLPAHASLGMDAVEAPRDASLHEALALISQAQKPVIYGGGGIVLGDAVQA
                     FRTFVDATQIPTVLTLKALGALATAHPLHLGMIGMHGSRAANLAVQESDLLIVVGARF
                     DDRATGKLAEFAPNARVVHMDIDACEIGKLRHADAGVRGDIATTLIRLTLPCAAHLHG
                     RHGTARKAWRAQCQQRARQHAARYDAPGETVFAPALLKRLSELAPEAIVSCDVGQHQM
                     WVAQHWRMDDPRKHLTSGALGAMGFGLPAAMGAQEAMRNAGRPDAQVICVSGDGSIMM
                     NIQELATLKRYRLPVKVVLLDNQAL"
     gene            complement(1426..2445)
                     /gene="ilvC"
                     /locus_tag="PK399_12610"
     CDS             complement(1426..2445)
                     /gene="ilvC"
                     /locus_tag="PK399_12610"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPW14003.1"
                     /translation="MTSSSKPRIAIVGYGSQGRAHALNLRDSGFDVTIGLRPGGPTEL
                     KAKADGFTVKTPADAVKDAEIVAILTPDMVQPQLYGEVIEPNMAPGACLLFAHGFNVH
                     YGQITPREDLDVVLVAPKGPGALVRREYEIGRGVPSVYAIHQDKSGNAEQFALTYCAG
                     IGGARQNAIKTTFKEETETDLFGEQAVLCGGATKLVQAGWETLVEAGYQPEVAYYECL
                     HELKLIVDLFYEGGITRMHEFISETAQYGALTRGNYVVDDNTRAQMKKVLTEIQDGTF
                     ARQWIAEYAAGNANYKAMKQADLDHPIEAIGKKLRANMKWLASSQAAPAKQPATDAQP
                     QSEAA"
     gene            complement(2442..2696)
                     /locus_tag="PK399_12615"
     CDS             complement(2442..2696)
                     /locus_tag="PK399_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14004.1"
                     /translation="MVFSLHVATPHIADDPTDRPCGVDPRTRPYYLANRCGTIRRVSR
                     NTTPESQTPHRKARGFSFLGPEKQKKNHHATIEPNPGQTQ"
     gene            2828..>3209
                     /locus_tag="PK399_12620"
     CDS             2828..>3209
                     /locus_tag="PK399_12620"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPW14005.1"
                     /translation="MPEYRSKTSTAGRNMAGARALWRATGMTDGDFHKPIIAIANSFT
                     QFVPGHVHLKDLGQLVAREIERVGGVAKEFDTIAVDDGIAMGHDGMLYSLPSREIIAD
                     SVEYMVNAHCADALVCISNCDKITP"
BASE COUNT          486 a         1035 c         1097 g          591 t
ORIGIN      
        1 ccagcgcctg gttgtcgagt agcacgacct tcaccggcag ccggtagcgc ttcagcgtgg
       61 ccagctcctg gatattcatc atgatcgagc cgtcaccgct cacgcagatc acctgcgcgt
      121 cggggcggcc tgcattgcgc atcgcttcct gcgcgcccat cgccgcgggc aggccgaagc
      181 ccatcgcgcc cagcgcaccc gacgtcaggt gcttgcgcgg gtcgtccatg cgccagtgct
      241 gggccaccca catctggtgc tggccgacat cgcaggacac gatcgcctcg ggcgccagtt
      301 ccgaaaggcg cttcagcagc gccggggcga acacggtctc gccgggggcg tcgtagcgcg
      361 ccgcgtgctg gcgcgcgcgt tgctggcatt gcgcgcgcca tgccttgcgg gcggtgccgt
      421 ggcggccgtg caggtgggcg gcgcagggca gggtcagcct gatcagggtg gtggcgatgt
      481 cgccgcgcac gccggcatcg gcatgccgca gcttgccgat ctcgcaggcg tcgatgtcca
      541 tgtggacgac gcgtgcgttc ggtgcgaact cggcgagctt gccggtggcg cgatcgtcga
      601 agcgcgcgcc gaccacgatc agcaggtcgg attcctggac tgccaggttg gcggcgcggc
      661 tgccatgcat gccgatcatg ccgaggtgga gcggatgcgc ggtggccagc gcgcccagtg
      721 ccttcagggt cagcacggtc gggatttgcg tggcatcgac gaaggtgcgg aacgcctgca
      781 ccgcatcgcc gaggacgatg ccaccgccgc catagatgac cggcttctgc gcctgcgaga
      841 tcagcgccaa tgcttcgtgc agcgaggcat cgcgaggcgc ctcgacggca tccattccga
      901 gcgaggcatg ggcaggcaga tgcgacgcat ccgccatctg cacgtccttg ggcagatcga
      961 tcagcaccgg gccggggcgg ccggaacgcg ccagccggaa cgcctcggcg accatcatcg
     1021 gcaggtcctc gacacggcgg gcgatgaagc tgtgcttcac catcggcagg gtcatgccga
     1081 acacgtccag ttcctggaat gcgtcggtgc ccatcaggtg ggtcggcacc tggccggtga
     1141 tgatgaccat cgggaccgaa tccagcatcg catcggcgat gccggtcacc aggttcgatg
     1201 cgcccggccc ggaggtggcc acgcacacgc cgacgcgccc gctggcacgc gcatacccgt
     1261 tggcggcgaa cgccgcgccc tgttcgtggc gtaccagcac gtgcttcagt gcgggtgcgc
     1321 tgtgcagggc gtcgtagaag ggcatgatcg tgccgccggg atacccgaac agcgtttcca
     1381 cgccttccgc gaccagggct tgcgccagcc agcgggctcc attcatcagg cggcctcgct
     1441 ctgcggctgc gcatcggtcg ccggttgttt cgccggtgct gcctgcgagc tggccagcca
     1501 cttcatgttg gcgcgcagct tcttgccgat cgcctcgatc ggatggtcca ggtcggcctg
     1561 cttcatcgcc ttgtagttgg cgttgccggc ggcgtattcg gcgatccact ggcgcgcgaa
     1621 ggtgccgtcc tggatttcgg tcaggacttt cttcatctgc gcgcgggtgt tgtcgtcgac
     1681 cacgtaattg ccacgggtca gtgcgccgta ctgcgcggtc tcgctgatga attcgtgcat
     1741 gcgggtgatg ccgccctcgt agaacaggtc gacgatcagc ttcagttcgt gcaggcactc
     1801 gtaatacgcg acctccggct ggtagccggc ttcgaccagg gtttcccagc cggcctggac
     1861 caacttggtc gcgccaccgc agagcacggc ttgctcgccg aacaggtcgg tttcggtttc
     1921 ttccttgaag gtggtcttga tcgcgttctg gcgcgcgcca ccgatgccgg cgcagtaggt
     1981 cagcgcgaac tgctcggcat tgccactctt gtcctggtgg atcgcataga ccgatggcac
     2041 gccgcggccg atctcgtatt cgcgacggac cagcgcgccg gggcccttgg gcgcgaccag
     2101 caccacgtcc aggtcctcgc gcggggtgat ctggccgtag tggacgttga aaccatgcgc
     2161 gaacaacagg caggcgcccg gtgccatgtt tggctcgatg acttcgccgt acagctgcgg
     2221 ctgcaccatg tccggggtca ggatcgcgac gatctcggcg tccttcaccg catcggccgg
     2281 cgttttcacg gtgaagccgt cggccttcgc cttgagttcg gtcggcccgc ccgggcgcag
     2341 gccgatggtg acgtcgaagc cggagtcgcg gaggttgagc gcatgtgcgc ggccctggct
     2401 gccgtagccg acgatggcga tgcggggctt gctgctgctg gtcattgcgt ctgtcctggg
     2461 ttgggttcga tggtggcgtg gtggtttttc ttttgttttt ccgggcccag aaacgaaaaa
     2521 ccccgcgcct ttcggtgcgg ggtttgcgat tctggcgttg tgtttctgct tacgcgccgg
     2581 atcgtcccgc acctgttggc gaggtaataa ggacgagtac gaggatcgac gccgcagggg
     2641 cggtcggtgg ggtcgtcggc gatgtgcggc gttgcaacat ggagcgagaa gaccatgcgc
     2701 cgcgcggtgg tgtcaacagg tcggcttatg ccgatttcgc atgaacgacg gttgcacctg
     2761 aaaccgtgga aacccagcag aatcaaggct tccagcccgc caagcacaat cgcgaagccg
     2821 cacgccgatg cccgagtacc gttcgaagac ctccaccgcc ggccgcaaca tggccggtgc
     2881 ccgcgcgttg tggcgcgcca ccggcatgac cgatggcgac ttccacaagc cgatcatcgc
     2941 gatcgccaat tcgttcaccc agttcgtgcc cggccacgtg cacctgaagg atctcgggca
     3001 actggtcgcg cgtgagatcg aacgcgtcgg cggcgtggcc aaggagttcg acaccatcgc
     3061 cgtcgatgac ggcatcgcga tgggccacga cggcatgctg tactcgctgc cgtcgcgcga
     3121 aatcatcgcg gactcggtgg agtacatggt caatgcgcat tgcgcggacg cgctggtgtg
     3181 catttccaac tgcgacaaga tcacgcccg
//
LOCUS       DAOOCX010000216         3148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_30110_length_3148_cov_8.351762, whole genome shotgun sequence.
ACCESSION   DAOOCX010000216 DAOOCX010000000
VERSION     DAOOCX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30110_length_3148_cov_8.351762"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /gene="coaE"
                     /locus_tag="PK399_12625"
     CDS             <1..405
                     /gene="coaE"
                     /locus_tag="PK399_12625"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914875.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPW14006.1"
                     /translation="PAMRRRVFADPDARRRLEAIVHPRVREALRASCSAAPGPYAIAS
                     IPLLAEGGGRDAYPWLQRILVIDLPEAMQLARLLQRDGIDDALARTMIAAQASRRERL
                     AIADDVLVNDGPLEALPPQVEALDRLYRRLAA"
     gene            complement(402..1361)
                     /locus_tag="PK399_12630"
     CDS             complement(402..1361)
                     /locus_tag="PK399_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HPW14007.1"
                     /translation="MKQIHVVAGVLRNTHGHVLLARRTEGRDLAGLWEFPGGKVEAGE
                     SPEVALVRELREELGIEATVGASLIRVPQQYPGKRLLLDARHVEFEGVPEGLDGQALA
                     WVPQEDLVGYPMPPADMPVVAALIQPDRYLVTPAPGDDDEAWLASLADALARDVRRVQ
                     LRAPGVAARRWSGLVADAIALCRDASAEVLLNGDPDWARQAGVGVHLRSAQLHDVALG
                     AALEGVRQAGHAIAASCHDAADLKRAEALGCSFVVLGPVQATSSHPGEPGIGWEAFAG
                     LREHVALPIYAIGGLAPAEIAKARTHGAQGIAAIRALWEAGRA"
     gene            complement(1560..>3148)
                     /locus_tag="PK399_12635"
     CDS             complement(1560..>3148)
                     /locus_tag="PK399_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPW14008.1"
                     /translation="LSGMTGTADTEAYEFQNIYGLEVVVIPTHRPMIRKDHSDLVFLN
                     RAGKYRAVANEIKACAAKGQPVLVGTTSIEVSELLSDALNEAGIAHEVLNAKQHEREA
                     HIVANAGAPGAVTIATNMAGRGTDIVLGGSLEAELAALGEDVSPSEKERIKAAWQVRH
                     DAVKAAGGLHIVGTERHESRRIDNQLRGRSGRQGDPGSSRFYLSLEDNLMRIFAADWV
                     QKVMARMGLKEDDIIESPLVTKQIANAQRKVEAHNFDIRKNLLDFDDVNNDQRKVIYG
                     QRDELLEADSVKANIDGIRSDVVADLVERFVPADSVDDQWDLPGLEAELATEFALQLP
                     LVRMHQEQDELDASQILAKVQESVEELFQAKEAQVGGETMRMLEKHVMLNVLDQNWKE
                     HLGRMDYLRQGIHLRGYAQKQPKQEYKKEAFELFSELLEKVKREVISLLARIRVRDEA
                     EVAAAEAEERARAEAVARQMQFQHPDMGGLGADEEAAAVQQLGQQPQGYGEAFDRIGR
                     NDPCPCGSGKKFKHCHGQLA"
BASE COUNT          454 a         1115 c         1048 g          531 t
ORIGIN      
        1 ccggccatgc gccgccgcgt gttcgccgac cccgatgcgc gacggcgact ggaggcgatc
       61 gtccatccac gcgtgcgcga ggcgctgcgg gcgtcctgca gtgccgcgcc ggggccctac
      121 gcgatcgcct cgatcccgct gctggccgaa ggcggcggtc gcgatgccta tccgtggctg
      181 cagcgcatcc tggtgatcga cctgccggaa gcgatgcaac tggcccgact gctgcagcgc
      241 gacggcatcg acgatgccct ggcccgcacg atgatcgcgg cgcaggcgag ccggcgggaa
      301 cggctggcga ttgccgacga cgtgctggtc aacgatggcc cgctggaggc gctgccgccg
      361 caggtggaag cgctggatcg gctgtaccga cgcctggcgg cctaggcgcg ccctgcctcc
      421 cacagggcac ggatcgcggc gatgccctgg gcgccgtggg tacgcgcctt ggcgatctcc
      481 gcgggcgcga ggccgccgat cgcatagatc ggcaatgcca catgttcgcg caagccggcg
      541 aatgcctccc agccgatccc gggctcgccg ggatgcgagg aggtcgcctg caccgggcca
      601 agcacgacga aggagcagcc gagtgcctcc gcgcgcttca ggtcggctgc gtcatggcag
      661 gaggcggcga tggcgtgacc cgcctgccgc acgccctcca gggccgcccc gagtgccaca
      721 tcgtgcagct gggccgagcg caggtgcacg ccaacccccg cttgcctggc ccagtcggga
      781 tctccattga gcaggacctc ggcactcgca tcgcggcaca gcgcgatggc atcggcgacc
      841 aggccactcc aacgacgcgc cgcgacaccc ggcgcgcgca actgcacgcg acgcacgtcg
      901 cgcgccagcg catccgccag tgacgccagc caggcctcgt catcatcgcc aggcgccggc
      961 gtgaccaggt agcgatccgg ctggatcaac gcggcgacca ccggcatgtc cgcgggcggc
     1021 atcgggtaac cgaccagatc ctcctgcggc acccaggcca gcgcctgccc gtcgagtccc
     1081 tcgggcacgc cctcgaattc gacatggcgc gcgtccagca gcagccgctt gccgggatac
     1141 tgctgcggca cgcggatcag cgatgcgccg accgtggcct cgatgccgag ttcctcgcgc
     1201 agctcgcgga ccagcgcgac ttcaggggac tcgcccgctt ccaccttgcc acccgggaat
     1261 tcccacaggc cggcgaggtc gcggccttcg gtacgacgcg ccagcaggac gtggccatgc
     1321 gtattgcgga ggacgccggc gacgacgtga atttgcttca tcgagttgcg aaaaggggac
     1381 gaagagaatc aagcgagctt aattctctcc gaccgccgtt ctctcggata ccgacgcccc
     1441 ccttggtaag gggggcgcgc cgaaggcgcg ggggctcgga agcacgaccc gtgggacccg
     1501 aggtcgcaca gcgaccttgg ggatccagcc cgcaggcggg ctggatcacg ggcagcttgc
     1561 tacgcaagct gcccgtggca gtgcttgaac ttcttgccgc tgccgcaggg gcagggatcg
     1621 ttgcggccga tgcggtcgaa ggcctcgccg tagccctgcg gctgctggcc gagctgctgc
     1681 acggccgcgg cttcctcgtc ggcacccagc ccgcccatgt ccggatgctg gaactgcatc
     1741 tgcctggcca ccgcctcggc gcgcgcgcgc tcctcggctt cggcggcagc gacttcggct
     1801 tcgtcgcgga cgcggatccg cgcgagcagg ctgatcactt cgcgcttgac cttctccagc
     1861 aactcggaga acagctcgaa cgcttccttc ttgtattcct gcttcggctg cttctgcgca
     1921 tagccgcgca ggtggatgcc ctggcgcagg taatccatgc gccccaggtg ttccttccag
     1981 ttctggtcga gcacgttgag catcacgtgc ttctccagca tgcgcatggt ctcgccaccg
     2041 acctgggctt ccttggcctg gaacagctcc tcgaccgact cctgcacctt cgccaggatc
     2101 tgcgacgcgt ccagctcgtc ctgctcctgg tgcatgcgca ccagcggcag ttgcagcgcg
     2161 aactcggtgg ccagctcggc ttccaggccc ggcaggtccc actggtcgtc gacagaatcg
     2221 gccggcacga agcgctccac caggtcggcc accacgtcgc tgcggatgcc gtcgatgttg
     2281 gccttgacgc tgtccgcctc cagcaactcg tcgcgctggc catagatcac cttgcgctgg
     2341 tcgttgttga cgtcgtcgaa gtccagcagg ttcttgcgga tgtcgaagtt gtgcgcctcc
     2401 accttgcgct gcgcgttggc gatctgcttg gtgaccagcg ggctttcgat gatgtcgtct
     2461 tccttcaggc ccatccgcgc catcaccttc tgtacccagt cggcggcgaa gatgcgcatc
     2521 aggttgtctt ccagcgacag gtagaagcgg gacgaacccg gatcgccctg gcggccggaa
     2581 cgcccgcgca gctggttgtc gatgcggcgg ctttcgtggc gctcggtgcc gacgatgtgc
     2641 aggccgccgg ccgccttgac cgcgtcgtgg cggacctgcc atgcggcctt gatccgctcc
     2701 ttctccgacg gcgacacatc ctcgccgagc gcggcgagtt ccgcttccag cgagccgccc
     2761 agcacgatgt cggtaccgcg acccgccatg ttggtggcga tcgtcaccgc gcccggcgca
     2821 ccggcgttgg cgacgatgtg cgcttcgcgc tcgtgctgct tggcgttcag cacttcgtgg
     2881 gcgatgccgg cctcgttcag cgcgtcgctc agcaattccg acacttcgat cgaggtggtg
     2941 ccgaccagca ccggctggcc cttggcggcg caggccttga tctcgttggc caccgcgcgg
     3001 tacttgccgg cgcggttgag gaacaccagg tccgaatgat ccttgcggat catcgggcga
     3061 tgggtgggga tcaccaccac ttccagcccg tagatgttct ggaattcgta ggcttcggtg
     3121 tccgcggtgc cggtcatgcc ggacagct
//
LOCUS       DAOOCX010000217         3096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_30844_length_3096_cov_5.572180, whole genome shotgun sequence.
ACCESSION   DAOOCX010000217 DAOOCX010000000
VERSION     DAOOCX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30844_length_3096_cov_5.572180"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="PK399_12640"
     CDS             complement(<1..1305)
                     /locus_tag="PK399_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPW14009.1"
                     /translation="MPDSTVRDTSKPVLITDDAALDRYLADVQRGAYGTRRMPAGVEL
                     ADWLASGDDACERCYMHASRLDVPLVRLPAVVGDPEVAKLLPPAKARQLRVVPLRAAQ
                     GLLAVAMEDPSDSEALAAVEFVTTNRVVPLMGSARGIRAAIARSYDLAEDRETARRLG
                     LDPLASIDASEAEAQRLAREQPVVRLVHGLIADAVARRASDIHLRPGEEATEVLFRID
                     DELVPVRSLLRALHAAVVSRIKVLGGMNLAEHRRAQDGRTSFILDDGTEVDLRISVLP
                     AVFGESAVVRLLDTRESLWTLEQLGLAEADRLRIDDVMSRSHGMFLATGPTGCGKSTT
                     LYAMLLELRQQRINILTIEDPVEFHIPEIQQMQVNRAAGFTFSSAMRNFLRHDPDVIM
                     VGEIRDRETADIAVESALTGHLLLSTLHTNTAASTITRLLDLG"
     gene            complement(1566..2756)
                     /locus_tag="PK399_12645"
     CDS             complement(1566..2756)
                     /locus_tag="PK399_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPW14010.1"
                     /translation="MNAALNDYLRQMVDARASDLFFSVGAPPTLKIDGTLHPLDEAAL
                     TPGVVTELALQAMNDRQKREFDETMEMNLALDVRDAGRCRVNIYRQRGNPAIAIRFIT
                     NRIPSLEELQLPALLQDLALEPRGLVLVVGSAGAGKSTTLASMIDHLNTHHQVHILTV
                     EDPIEYLHTHKKSIVDQREVGLDTQSFANALRNTMREAPDVLMIGEIRDRETMQAAIS
                     YAETGHLCMATLHANNANQALDRIVNFFPDTAHHQLFVDLSLNLKAVVSQRLLRTVDG
                     QRVPAVEILLRSQFVAELIGKGEIASIHDAMKQSSELGMLLFDDSIYRLYAAGRISYE
                     EAMDNADSRTDLGVRIRLEGPRPPGEHHDELEGARIQGIDADGRVVEHASAHDGTYLN
                     VRGG"
BASE COUNT          456 a         1023 c         1079 g          538 t
ORIGIN      
        1 gcccaggtcg agcaggcggg tgatggtcga tgcggcggta ttggtatgca gcgtgctgag
       61 cagcaggtgg ccggtcagcg cgctctccac ggcgatgtcg gcggtctcgc ggtcgcgtat
      121 ctcgccgacc atgatcacgt ccgggtcgtg gcggaggaag ttgcgcatcg cgctggagaa
      181 ggtgaagccc gccgcgcggt tgacctgcat ctgctggatc tccgggatgt ggaattccac
      241 cggatcctcg atggtgagga tgttgatccg ctgctggcgc aattccagca gcatcgcgta
      301 cagggtcgtg gacttgccgc aaccggtcgg cccggtcgcc aggaacatgc cgtggctgcg
      361 gctcatgacg tcgtcgatgc ggaggcggtc tgcctccgcc aggcccagtt gctcgagcgt
      421 ccacaggctc tcgcgcgtgt ccagcaggcg caccaccgcg ctctcgccga acactgccgg
      481 cagcaccgag atgcgcaggt cgacctcggt gccgtcgtcc aggatgaagc tggtgcggcc
      541 gtcctgcgcg cgccggtgct cggccaggtt catgccgccg agcaccttga tccggctcac
      601 caccgcggcg tgcagggcgc gcagcaggct gcgcaccgga accagttcgt cgtcgatccg
      661 gaacaggacc tcggtcgcct cttcgccggg gcgcaggtgg atgtcggagg cgcgccgggc
      721 caccgcgtcg gcgatcagcc cgtgtaccag ccgcaccacc ggttgctccc gcgcaagtcg
      781 ctgcgcctcg gcttcgctgg catcgatgct ggccagggga tccaggccga gccggcgtgc
      841 ggtctcgcga tcctcggcca ggtcgtagct gcgtgcgatg gccgcgcgga tgccacgcgc
      901 cgaccccatc agcggcacga cccgattggt ggtgacgaac tcgaccgcgg ccagcgcctc
      961 gctgtcggat ggatcttcca tcgccaccgc gagcaacccc tgcgccgccc gcagcggaac
     1021 cacccgcaac tgccgggcct tggcgggcgg caacagcttg gcgacttcgg gatcgcctac
     1081 gacagcggga agccgaacca ggggtacgtc gaggcgactg gcgtgcatgt agcagcgctc
     1141 gcaggcgtcg tcgccgctgg ccagccagtc ggcaagctcc accccggccg gcatgcggcg
     1201 ggtcccgtat gcgccgcgct ggacatccgc caggtagcgg tccagcgcgg cgtcatcggt
     1261 gatcaggacg ggcttgcttg tgtcgcgtac ggtggaatcg ggcatcagcg gttccagtgt
     1321 ggctccccca cagtctgggc tggcgggcgt ccgcggcatt gatcccgatc aactcgggcg
     1381 cggtgatatt cgggatcccg gcccgccgtc gcgtgcaagg cgccaggcac tcgcttgaag
     1441 tgcgggatca ctggatggtc gggaacacga ggcagggagc gcgatgcggg tgaacgcgca
     1501 gtcccggctc cagcgcggct cgcgacccgg cacggcggga ttccgcgcgc cggccccgca
     1561 cgtcttcatc ccccgcggac gttgagatag gttccgtcgt gagccgacgc gtgctcgacg
     1621 acccgcccgt ccgcgtcgat cccctggatc cgcgcgccct cgagttcgtc gtggtgctcg
     1681 ccgggcggcc gaggaccttc cagcctgatc cgcacgccca ggtcggtgcg cgaatcggcg
     1741 ttgtccatgg cttcctcgta actgatccgg cccgctgcat agagccggta gatcgagtcg
     1801 tcgaacagga gcattccgag ctcggagctt tgcttcatgg cgtcgtggat ggaggcgatc
     1861 tcgccctttc cgatcagttc ggcgacgaac tgactccgca acaggatttc caccgccggc
     1921 acacgctgtc cgtccacggt gcgcaacagg cgctgcgaca ccacggcctt gaggttgagc
     1981 gacaggtcga cgaacaactg gtgatgggcg gtgtccggga agaagttgac gatgcggtcc
     2041 agcgcctggt tggcattgtt cgcgtgcagc gtggccatgc acaagtggcc ggtttccgca
     2101 tacgagatcg ccgcctgcat ggtttcccgg tccctgatct cgccgatcat cagcacgtcg
     2161 ggggcctcgc gcatggtgtt gcgcagggcg ttggcgaaac tctgcgtgtc caggccgacc
     2221 tcgcgctggt cgacgatcga cttcttgtgg gtgtggaggt attcgatcgg atcctcgacg
     2281 gtcaggatgt gcacctggtg atgcgtgttg aggtggtcga tcatcgaggc cagggtcgtg
     2341 gacttgcctg cgcccgcgct gcccaccacc agcaccagcc cgcgtggctc gagcgcaaga
     2401 tcctgcagca atgcgggcag ctggagttcc tccaacgaag gaatccggtt ggtgatgaag
     2461 cggatcgcga tcgccggatt gccgcgctgg cggtagatat tgacgcggca gcggccggcg
     2521 tcgcgcacgt cgagggcgag gttcatctcc atcgtctcgt cgaattcgcg tttctgccgg
     2581 tcgttcatgg cttgcagcgc cagctcggtg accacgcccg gcgtcaacgc ggcctcgtcg
     2641 agcgggtgca gcgtgccatc gatcttcagc gtgggtggcg cgccaaccga aaagaagagg
     2701 tcggacgccc tggcgtccac catctggcgc aggtagtcgt tcagcgcggc attcattggc
     2761 gtgtcctcgc tgccggccgg cagccattgg aggggaacgg atccggttgt cgccacgaac
     2821 gccagaatgt cgcccgtgcg ccccatcgtt ctccgatcgc ggccctcggg cattgatctg
     2881 gatcaacatg ccggtcggcc ttggatcgct tgcgtcgcga tcagcgaccg agtcacgggg
     2941 attcaccgcc ggccgcgcat ggtcgagcca ggctcgtccg ttccatccgg gtcgatcggg
     3001 ggggcgcctc cccgcatacc ccggcatgga cgcgccggca ccccgggcgg aagcgggtgg
     3061 tgtccaggaa gaacggatcc tgctggccga cgtccg
//
LOCUS       DAOOCX010000218         3066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_31254_length_3066_cov_8.954500, whole genome shotgun sequence.
ACCESSION   DAOOCX010000218 DAOOCX010000000
VERSION     DAOOCX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31254_length_3066_cov_8.954500"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="PK399_12650"
     CDS             complement(<1..1394)
                     /locus_tag="PK399_12650"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPW14011.1"
                     /translation="MTDPTPSDVTAHGTQRIKIFDTTLRDGEQSPGFTMNAAQKVLFA
                     HALEDLGVDVIEAGFPNSSPADFGAVQSIAREIRHASICALARCHPADIDACGSALQG
                     AAAPRIHVFISTSPLHRQHKLGMSEDQVIEHAVMGVTRARSHVEDVEFSAEDALRTEP
                     EFLVRVFEAAIEAGARTLNVPDTVGYTTPGEIRALFEYLRANVRGADGVVFSAHCHND
                     LGLAVANSLAAIEGGARQVECTINGIGERAGNAAMEELAMALRVRPGYYGADTRLDTT
                     KLLATSRLLSRITGIQVQRNKAIVGLNAFAHESGIHQHGMLKNRGTYEIMRPQDVGWE
                     DSQLVLGRHSGRAAVANRLRALGYVLEDAQLDQVIAEAKALTHTQHVITDSDLQRLVE
                     GEHVGPGWRIAGMGLSDNAGTTHARVDLSDPDGRHVVQSADGDGPVAALFAALAHATK
                     VDFVLESYEVHSMGV"
     gene            complement(1391..2515)
                     /locus_tag="PK399_12655"
     CDS             complement(1391..2515)
                     /locus_tag="PK399_12655"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HPW14012.1"
                     /translation="MDDSVARAVQVTASDVLAAQARLRRYLNPTPLHYAERFGCWLKL
                     ENLQRTGSYKVRGAMNALLAARERGDNRPTIAASAGNHAQGLAWAAYRLGMPAIAVMP
                     KTAPDTKVAGVAHWGAAVRLHGETYDEAKAFAAELAAQHGYRLLPAFDDPDVIAGQGT
                     VGLELASLMPDVVLVPIGGGGLAAGVVLALKSQGVKVIGAQVEGVDAMARALKGDTSL
                     RDPVATLADGVRVKEPGHITRVVLHELLDDIVIVREAELRETLVRLALEEHVIAEGAG
                     ALALAAGRRVAGKRKCAVVSGGNLDAGVLAALLQDIRPRPPRKPRRRVQEPLPAIVSA
                     TPVLDIAEARAGAARLRPAAANRFATPSPAIACTTEDCTT"
     gene            complement(2515..>3066)
                     /locus_tag="PK399_12660"
     CDS             complement(2515..>3066)
                     /locus_tag="PK399_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPW14013.1"
                     /translation="LDVEGHAPHMMVATTATQVHLNGTRARLGVSSWRRNPADSLPPL
                     KLCGAYVNSILAKREAKARGFDEAMFTDRSGNVVECTGANLFMVRDGSVVAIEHPDAL
                     PGITRDSLIALTGAASRMATLDELLDADEVFACGTAAEVAPISQVEDREYGTNPVTRA
                     IAEHYKRVVRGEDTSYRHWLTAV"
BASE COUNT          430 a         1050 c         1078 g          508 t
ORIGIN      
        1 acgcccatgc tgtgcacttc gtagctttcc agcacgaagt cgaccttggt ggcatgcgcc
       61 agcgcagcga acagcgcggc gaccgggccg tcgccgtcgg cgctctgcac cacgtggcgg
      121 ccgtcggggt cggagaggtc gacgcgcgcg tgggtggtgc cggcgttgtc ggacaggccc
      181 atgccggcga tccgccagcc ggggccgaca tgctcgcctt cgaccagccg ctgcaggtcg
      241 ctgtcggtga tcacgtgctg ggtgtgggtc agcgccttcg cctcggcgat cacctggtcc
      301 agctgcgcgt cttccagcac gtagcccagc gcgcgcaggc gattggcgac cgcggcgcgg
      361 ccactgtggc ggcccagcac cagctgcgaa tcctcccagc cgacgtcctg cggccgcatg
      421 atttcgtagg tgccgcggtt cttcagcatg ccgtgctggt ggatgccgga ttcatgcgcg
      481 aatgcgttga gaccgacgat cgccttgttg cgctggacct ggatgccggt gatccgcgac
      541 agcaaccgcg acgtggccag caacttcgtc gtgtccagcc gggtgtccgc gccgtagtag
      601 ccggggcgca ccctcagcgc catcgccagc tcctccatcg ccgcgttgcc ggcacgttcg
      661 ccgatgccgt tgatggtgca ttcgacctgg cgcgcaccgc cctcgatcgc ggccagcgag
      721 ttcgcgacgg ccaagcccag gtcgttgtgg cagtgcgcgc tgaagaccac gccgtcggcg
      781 ccgcgcacgt tggcacgcag gtattcgaac agcgcgcgaa tttcgcctgg cgtggtgtaa
      841 ccgacggtgt ccggcacgtt gagcgtgcgc gcgccggctt cgatggcggc ctcgaagacg
      901 cggaccagga attcgggttc ggtgcgcagc gcgtcctcgg cggagaactc cacgtcctcg
      961 acgtggctgc gcgcgcgggt gacgcccatc accgcgtgtt cgatgacctg gtcttcgctc
     1021 atgcctagct tgtgctggcg gtgcagcgga ctggtggaga tgaacacgtg gatgcgcggc
     1081 gcggcggcac cctgcaaggc gctgccgcag gcgtcgatat cggcggggtg gcaacgcgcc
     1141 agcgcgcaga tggacgcgtg gcggatctcg cgggcgatgc tttgcacggc gccgaaatcc
     1201 gcgggggaac tgttggggaa gccggcctcg atcacgtcca cgccgaggtc ctccagcgca
     1261 tgcgcgaaca ggaccttctg cgcggcgttc atggtgaagc ccggcgattg ctcgccatcg
     1321 cgcagggtgg tgtcgaaaat cttgatgcgt tgcgtgccgt gtgcggttac gtcgctcggg
     1381 gtcgggtcgg tcacgtcgtg caatcctcgg tggtgcatgc gatggcgggg cttggtgtcg
     1441 cgaagcggtt ggccgccgcc ggcctaagtc gcgccgcacc ggcgcgcgcc tcggcgatgt
     1501 ccagcactgg cgttgcgctg acgatggccg gcaacggttc ctgcacgcgc cggcgcggct
     1561 tgcgcggcgg acgcgggcgg atgtcctgca gcagcgcggc gaggacgccg gcatcgaggt
     1621 tgccgccgga caccaccgcg cacttgcgct tgccggccac gcggcggccc gcggccaggg
     1681 ccagcgcgcc ggcaccctcg gcgatcacgt gttcttccag cgccaggcgc accagggttt
     1741 cgcgcagctc ggcctcgcgc acgatcacga tgtcgtcgag taattcatgc agcaccacgc
     1801 gggtgatatg gcccggttcc ttgacccgca cgccatcggc gagggtcgcg acgggatcgc
     1861 gcaggctggt gtcgcccttc aacgcacgcg ccatcgcatc cacgccttcg acctgcgcgc
     1921 cgatcacctt cacgccctgc gatttcaatg ccagcacgac gcccgcggcc aagccgccac
     1981 cgccgatcgg caccagcacc acatccggca tcagcgacgc aagttccagc ccgaccgtgc
     2041 cttgcccggc gatgacatcc gggtcatcga acgcgggcag gaggcgatag ccgtgctgcg
     2101 cggccagttc cgcggcgaaa gccttggctt cgtcgtaggt ctcgccatgc aggcgcacgg
     2161 cggcgcccca atgggcgaca ccggccacct tggtgtccgg cgcggtcttc ggcatcaccg
     2221 cgatcgccgg catgcccagg cgatacgcgg cccaggccag gccctgtgca tggttgccgg
     2281 cggacgcggc gatggtcggg cggttgtcgc cgcgttcgcg cgcggcgagc agcgcgttca
     2341 tcgcgccgcg caccttgtag gagccggtgc gctgcaggtt ctccagcttc aaccagcagc
     2401 cgaagcgttc ggcgtagtgc agcggcgtgg gattgaggta acgacgcaac cgagcctgcg
     2461 ccgccagcac gtcgctggcg gtcacctgca cggcgcgggc taccgaatcg tccatcagac
     2521 tgcggtcagc caatggcgat acgacgtgtc ttcgccacgc accacgcgct tgtagtgctc
     2581 ggcgatggcg cgagtcaccg ggttggtgcc gtattcgcgg tcttcgacct gcgagatcgg
     2641 cgcgacctcg gcggcggtgc cgcaggcgaa gacttcatcg gcgtccagca attcgtccag
     2701 cgtggccatc cgcgatgccg cgccggtcag cgcgatcagg ctgtcgcggg tgatccccgg
     2761 caacgcatcc ggatgctcga tggcgacgac gctgccgtcc ctgaccatga acaggttggc
     2821 gccggtgcac tcgaccacgt tgccgctgcg gtcggtgaac atcgcctcgt cgaagccgcg
     2881 cgccttcgct tcgcgcttgg ccaggatcga attgacgtag gcgccgcaca atttcagcgg
     2941 cgggagcgag tccgccgggt tgcgccgcca actggacacg cccagccgcg cgcgggtgcc
     3001 gttgaggtgc acctgtgtcg cggtggtggc gaccatcatg tgcggcgcgt ggccttcgac
     3061 atccag
//
LOCUS       DAOOCX010000219         3041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_31631_length_3041_cov_7.048560, whole genome shotgun sequence.
ACCESSION   DAOOCX010000219 DAOOCX010000000
VERSION     DAOOCX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31631_length_3041_cov_7.048560"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PK399_12665"
     CDS             complement(<1..1208)
                     /locus_tag="PK399_12665"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPW14014.1"
                     /translation="MSAPNHPHPGPLPPAGEVDWTRVRDNFPLLKREVHGKPLVYLDS
                     ANTGQKPAAVIEATDDFYRRHNANVSRAVHTLGSEATDAYEGARRKLAAFLNVQRDDL
                     VLCSGTTFAINLAAYSWALPRLKAGDTILITRMEHHANIVPWQLVAQRTGATIKVAEL
                     LPDGSLDLDALHAAMTADVKLLAIAHVSNVLGTVNPVAAICREARKRGIVTVVDGSQA
                     APHMAVDIPAIGCDFYAITGHKMCGPTGTGALWARREHLQAMPPFIGGGEMIKEVRFD
                     GTVFNDPPHKFEAGTPNIAGFIGLGAAVDYLSALGMDNVAAREGELLAHATEELRKVD
                     GLRIFGEARHKAAVISFQVEGAHAHDLATLLDLEGVAVRSGQHCAHPLLHWFGVAATC
                     RASLAFYNTH"
     gene            complement(1205..2494)
                     /gene="sufD"
                     /locus_tag="PK399_12670"
     CDS             complement(1205..2494)
                     /gene="sufD"
                     /locus_tag="PK399_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPW14015.1"
                     /translation="MSALLDSFAATFDALAARDAAGLGENRRSALDSVLRDGLPGPRS
                     EAWKYTSLRALERRGFIAPPATSIVDAEALAARIAAIPSPRMVFVDGRLDAGSSVLDG
                     LPDGIDVAPLSRVLHDGAPRDANILQRRYAGADQVFAVANAALAEEGAVIRVDRTTDT
                     TLHLVFVATSGDIGVHLRHLIDLRNDASLSLVEHHLALGEDRGLANHVSHVHLGQRAR
                     LHHVRLQDAAAGASLFARTDAVLARDADYRRTDLELGSGLSRHELNVRLEGDHANLQA
                     DGVLLADARRHVDTRLGIEHIARDTHCTLGWRGIAGQRGRAVFHGGIVIRAGADGSEA
                     NLSNKNLLLSATAEVDTQPVLEIHADEVKAAHGATVGQLDANALFYLRSRGLGEAEAR
                     ALLTGAFCREIVAGIDDAPTRSLCEDALDAALARLGA"
     gene            complement(2496..>3041)
                     /gene="sufC"
                     /locus_tag="PK399_12675"
     CDS             complement(2496..>3041)
                     /gene="sufC"
                     /locus_tag="PK399_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPW14016.1"
                     /translation="ERAAAGVFLAFQYPVEIPGVNNTYFLRAAANAQRKARGESELDS
                     MQFLKKVREKLAVLHLKDELLHRGVNEGFSGGEKKRNEIFQLALLEPKLAILDETDSG
                     LDIDALKSVADGVNALRSPDRAFLVITHYQRLLDYIKPDVVHVLADGRIVESGGPELA
                     LELEEHGYAWLEGRKAPGQAA"
BASE COUNT          463 a         1030 c         1051 g          497 t
ORIGIN      
        1 tcgtgcgtgt tgtagaacgc cagcgacgcg cggcaggtcg cggccacgcc gaaccaatgc
       61 agcagcggat gcgcgcaatg ctggccggaa cgcaccgcca cgccttccag gtcgagcagg
      121 gtggccaggt cgtgcgcatg cgcgccttcg acctggaagc tgatcaccgc cgccttgtgc
      181 cgtgcttcgc caaagatgcg caggccatcg accttgcgca gttcctcggt cgcgtgcgcc
      241 agcagttcgc cctcgcgtgc ggcgacgttg tccatgccca gcgcggacag gtagtcgacc
      301 gccgcgccca ggccgatgaa gccggcgatg ttgggcgtgc cggcctcgaa cttgtgcggc
      361 gggtcgttga acacggtgcc gtcgaaacgg acttccttga tcatctcgcc gccgccgatg
      421 aagggcggca tcgcctgcag atgctcgcgg cgcgcccaca gcgcgccggt accggtcggc
      481 ccgcacatct tgtggcccgt gatcgcatag aaatcgcagc cgatcgccgg gatgtccacc
      541 gccatgtgcg gcgcggcctg cgagccgtcg acgaccgtca cgatgccgcg cttgcgcgcc
      601 tcgcggcaga tcgccgcgac cggattgacc gtgcccagca cgttgctgac gtgggcgatg
      661 gcgagcaact tcacgtccgc tgtcatcgct gcatgcagcg cgtccaggtc cagcgacccg
      721 tccggcagca actcggccac cttgatcgtc gcgccggtcc gttgcgcgac caactgccag
      781 ggcacgatgt tggcgtggtg ctccatccgg gtgatgagga tggtgtcgcc ggccttgagc
      841 cgcggcagcg cccacgaata cgcggccagg ttgatcgcga aggtggtgcc gctgcacagc
      901 accaggtcgt cgcgctgcac gttgaggaac gcggccagct tgcgacgcgc gccttcgtag
      961 gcgtcggtgg cttcgctgcc gagcgtgtgc acggcgcggc tgacgttggc gttgtggcgg
     1021 cgatagaagt cgtcggtcgc ctcgatcacc gccgccggct tctgcccggt attggcggag
     1081 tcgagataca ccagcggctt gccatgcact tcacgcttga gcagcgggaa gttgtcgcgc
     1141 actcgcgtcc agtcgacctc tcccgctggc gggagagggc cggggtgagg gtggttcggt
     1201 gcgctcacgc ccccaatctc gccaacgccg catccagcgc gtcctcgcac aaggaacgcg
     1261 ttggtgcatc gtcgatgccg gccacgatct cgcggcagaa cgcgccggtc agtagggcac
     1321 gcgcctcggc ctcgcccagc ccgcgcgaac gcaggtagaa cagcgcattg gcatccagtt
     1381 ggcccaccgt cgcaccgtgc gcggccttca cttcgtcggc atggatttcc agcaccggct
     1441 gggtgtcgac ctcggcagtc gccgagagca gcaggttctt gttcgacagg ttcgcctcgc
     1501 tgccgtccgc gcccgcgcgg atgacgatgc cgccatggaa caccgcgcgg ccgcgttgcc
     1561 cggcaatccc gcgccagccc agcgtgcaat gggtatcgcg ggcgatgtgc tcgatgccga
     1621 ggcgggtatc cacgtggcgt cgagcatcgg ccagcagcac gccatcggcc tgcaggttcg
     1681 cgtggtcgcc ttccagccgc acgttcaatt catgccgcga caacccgctg cccagttcca
     1741 ggtcggtgcg gcggtaatcg gcgtcgcgcg cgagcacggc gtcggtacgc gcgaagagac
     1801 tggcgccggc ggcggcgtcc tgcaggcgca catgatgcag ccgcgcgcgc tggcccaggt
     1861 gcacgtggct cacatggttg gccaggcctc ggtcctcgcc cagcgcgagg tgatgctcga
     1921 caagcgacag ggaggcgtcg ttgcgcaggt cgatgagatg gcgcaggtgc acgccgatgt
     1981 cgccactggt ggcgacgaag accaggtgca gcgtcgtgtc ggtcgtacgg tcaacgcgga
     2041 tgacggcgcc ttcttccgcc aatgcggcgt tggccaccgc gaagacctgg tcggcgccgg
     2101 catagcgacg ctgcaggatg ttggcatcgc gcggcgcacc gtcgtgcagc acgcgcgaca
     2161 gcggcgcgac gtcgatcccg tccggcaatc cgtccagcac gctgctgccg gcatcaaggc
     2221 gaccatcgac gaagaccatg cgcggcgacg gaatcgcggc gatgcgcgcg gcgagcgctt
     2281 cggcatcgac gatggatgtt gcaggcggtg caatgaagcc gcggcgctca agcgcacgca
     2341 gcgaggtgta cttccaggcc tcgctgcgcg ggccgggcag gccatcacgc agcacactgt
     2401 ccaatgcgct gcggcggttc tcgccaaggc cggcggcatc gcgcgcggcg agcgcgtcga
     2461 acgtggcggc gaacgaatcg agcagggcac tcatgtcagg cggcctgccc cggagccttg
     2521 cggccttcca gccaggcata accgtgttct tccagctcca gcgccagttc cgggccgccg
     2581 ctttcgacga tgcggccatc ggccagcacg tggaccacgt ccggcttgat gtagtcgagc
     2641 aggcgctggt agtgggtgat caccaggaac gcgcggtccg gcgaacgcag ggcgttgacg
     2701 ccatcggcca cggacttcag ggcatcgatg tccaggccgg aatcggtctc gtccaggatc
     2761 gccagcttcg gttccagcag cgcgagctgg aagatctcgt tgcgcttctt ctcgccgccg
     2821 ctgaagcctt cgttgacgcc gcgatgcagc agttcgtcct tgaggtgcag cacggcgagc
     2881 ttctcgcgca ccttcttcag gaactgcatc gagtccagtt cgctctcgcc gcgcgccttg
     2941 cgttgcgcat tcgcggccgc gcgcaggaag taggtgttgt tcacgccggg gatctcgacc
     3001 ggatactgga aggccaggaa cacgccggcg gcggcgcgtt c
//
LOCUS       DAOOCX010000220         2863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_34589_length_2863_cov_5.127493, whole genome shotgun sequence.
ACCESSION   DAOOCX010000220 DAOOCX010000000
VERSION     DAOOCX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34589_length_2863_cov_5.127493"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PK399_12680"
     CDS             <1..761
                     /locus_tag="PK399_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW14017.1"
                     /translation="VWAPTAKFSVSADYHHWDIRDEVRLQSVDQLMRDEAACRTGGLD
                     INSGTCQAAISQITRGSAGTVQSIEVKKINIAKQTLDAVTVDMNYRQELGAFGTLGLS
                     GSWTRNLEHLLQDYPTDPVVDALNDPYWSTDPKYKANASVSWNKARWTTTLYANYIGP
                     TPNYRATLDPDGYAFAGAGRLGSYTTANASVNFDVTEDLKLSFLVNNLANRMPDMDVR
                     SYPGNSGEPYNSSNFNLLGRAYYVEAKWNFGKGK"
     gene            complement(844..2412)
                     /locus_tag="PK399_12685"
     CDS             complement(844..2412)
                     /locus_tag="PK399_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPW14018.1"
                     /translation="MNEAQRLYADAVDALNRRQWQAARAMGLRLIAMVPDHGGVHFVA
                     GVASLQLQQLPEGIRHLQRAVHLSPKRPDYAAQFARALAMARLTREALVAADAASAMG
                     PGDPATLDTLGVVYTQANEHAKAIALFEQAARKVPAHAAYRFNLGTSYTFLGDLEQAE
                     REYEACLALEPGHGKAHLALAQLRKATPDANHVARLQGLLVDAGDAAARMYLNLALAK
                     EQEDLGDDARAFAHLTAGKAAGGEGRGYRAERDVALFAAIEQATPAGTMSAPGSASEE
                     PIFVIGMPRSGTTLVDRILSSHPLVHSAGELQNFGVVLKRASRSRTPELLDVDTVRSA
                     ASLYWRQLGDAYVASTRPATAGKPRFVDKLPHNFLYAGFIARALPNARIICLRRNPMD
                     TCLGNFRQLFAQSTPYYDYSFDLLDTGRYYILFDRLMTHWRNTMPGRILEIDYESIVE
                     DQEGTTRRLLAHCGLDWDEACMRFNDNAAPVATASAVQVRQPLHRDALQRWKRYEPQL
                     VELKMLLEAAGVVV"
     gene            2520..>2863
                     /locus_tag="PK399_12690"
     CDS             2520..>2863
                     /locus_tag="PK399_12690"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HPW14019.1"
                     /translation="MRKFILAGLLAAVAFSASAQNPATSPVGKWKTLDDKTGKAMTIT
                     EVYEAKNGTLAAKIVENLGMPATCANCSDEHKGKPIVGMVTLWNLKANKDGTWGGGSG
                     YKPSEDREFKAKS"
BASE COUNT          524 a          953 c          918 g          468 t
ORIGIN      
        1 tggtgtgggc accgaccgcc aagttctcgg tcagcgccga ctaccaccac tgggatatcc
       61 gcgacgaagt ccgcttgcag agcgtcgacc agttgatgcg cgacgaagcg gcctgccgca
      121 ccggcggcct cgacatcaac tccggcacct gccaggcggc gatcagccag atcacccgcg
      181 gctcggccgg cacggtgcag tcgatcgagg tcaagaagat caacatcgcc aagcagaccc
      241 tggacgcagt cacggtggac atgaattacc gccaggagct gggcgcgttc ggcacgttgg
      301 gcctgtccgg ttcgtggacg cgcaacctgg agcacctgct gcaggactac ccgaccgacc
      361 cggtcgtcga tgcactcaac gatccgtact ggagcaccga cccgaagtac aaggccaatg
      421 cgtcggtgag ctggaacaag gcccgctgga ccaccaccct gtacgcgaac tacatcggcc
      481 cgacgccgaa ctaccgcgcg acgctggatc ccgatggcta tgccttcgcg ggtgctggcc
      541 gcctgggttc gtacacgacg gccaatgcca gcgtgaactt cgatgtcacc gaagacctga
      601 agctgtcgtt cctagtcaac aacctggcca accggatgcc ggacatggat gttcgttcgt
      661 atcccggcaa cagcggcgaa ccgtacaaca gcagcaactt caacctgctg ggccgtgcgt
      721 actacgtgga agcgaagtgg aatttcggca agggcaagta atcgcctttc gtcgatgaca
      781 ccatgaggaa ccccgccaat cggcggggtt tctttttgca cgccctgcgg gcgactgcgc
      841 gattcagacg acgacgccgg ccgcttccag cagcattttc agctcgacca actgcggttc
      901 gtaacgcttc cagcgttgca gggcgtcgcg atgcagcggc tgccgcacct gcaccgcgct
      961 ggcggtcgca accggcgctg cgttgtcgtt gaagcgcatg cacgcttcgt cccaatccag
     1021 cccgcaatgc gccagcaacc tgcgcgtggt gccttcctgg tcctccacga tggactcgta
     1081 gtcgatctcg aggatgcggc ccggcatggt gttccgccaa tgcgtcatca gccggtcgaa
     1141 gaggatgtag tagcgaccgg tgtcgagcag gtcgaaggaa tagtcgtagt aaggcgtgct
     1201 ctgcgcgaac aactggcgga aattccccag gcaggtgtcc atcgggttgc gtcgcaggca
     1261 gatgatccgc gcgttcggca gcgcgcgtgc gatgaagccg gcgtagagga aattgtgcgg
     1321 gagcttgtcg acgaaccgcg gcttgcccgc cgtcgccggg cgcgtgctgg cgacgtaggc
     1381 atcgccgagt tgtcgccaat acagcgaagc ggccgaacgc acggtgtcga cgtcgagcag
     1441 ctccggggtg cggcttctgg atgcacgttt gagcaccacg ccgaagttct gcaattcgcc
     1501 cgccgaatgc accagcggat ggctggacag gatgcgatcc accagcgtcg tgcctgatcg
     1561 cggcatgccg atgacgaaga tcggttcctc gctcgcgctg ccaggggcgc tcattgtccc
     1621 ggcgggcgtg gcttgctcga tcgcggcgaa cagggcgaca tcgcgctcgg ctcggtatcc
     1681 gcggccttcg ccgccggccg cctttcccgc agtgagatgc gcgaacgcgc gggcatcgtc
     1741 gccgaggtct tcctgttcct tggccagcgc gaggttgagg tacatccgcg ccgccgcatc
     1801 gcccgcatcg accagcaagc cctgcaatcg cgcgacatgg ttggcatcgg gggtggcctt
     1861 gcgcaactgc gccagtgcga ggtgcgcctt gccatgcccc ggctcaagcg ccaggcaggc
     1921 ctcgtattcg cgctcggcct gctccaggtc gcccaggaac gtgtacgacg tgccgaggtt
     1981 gaaacgatac gccgcatgcg ccggcacctt gcgcgccgcc tgttcgaaca gggcgatggc
     2041 cttcgcgtgt tcattggcct gggtatagac cactccaagc gtgtccaggg tagccggatc
     2101 cccgggcccc atcgccgaag ccgcatccgc cgcgaccagc gcttcgcggg tgaggcgcgc
     2161 catcgccagc gcgcgagcga actgcgcggc gtagtccggg cgcttggggc tgaggtgcac
     2221 ggcgcgttgc aggtggcgga tgccttccgg caactgctgc agctgcagcg acgccacgcc
     2281 cgccacgaaa tggacgcccc catggtccgg caccatcgcg atgaggcgca gccccatcgc
     2341 gcgtgccgcc tgccactggc gacgattcaa ggcatccacg gcatcggcgt acaggcgctg
     2401 cgcttcgttc atgcagccat cctaaccttc agcctccata cggcatcgga tgcggtataa
     2461 atgcgccact cggccaacgg gcgcgccgcg ccccgccatc tccctgggag gggaccacaa
     2521 tgcgcaaatt catcctcgcc ggcctgcttg ccgcggtcgc tttctctgcc tctgcccaga
     2581 acccggcgac gtcaccggtc ggcaagtgga agacgctgga cgacaagacc ggcaaggcga
     2641 tgaccatcac cgaagtctac gaagccaaga acggcaccct ggccgcgaag atcgtggaga
     2701 acctgggcat gcccgccacc tgcgcgaact gcagcgacga gcacaagggc aagccgatcg
     2761 tcggcatggt caccctgtgg aacctgaagg cgaacaagga tggtacctgg ggcggcggca
     2821 gcggctacaa gccatccgaa gaccgcgagt tcaaggccaa gtc
//
LOCUS       DAOOCX010000221         2862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_34611_length_2862_cov_5.570004, whole genome shotgun sequence.
ACCESSION   DAOOCX010000221 DAOOCX010000000
VERSION     DAOOCX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34611_length_2862_cov_5.570004"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PK399_12695"
     CDS             complement(<1..353)
                     /locus_tag="PK399_12695"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPW14020.1"
                     /translation="MASMIVRAVRLHLACLLLLLLAACASGGGQRVANVSAEPLPAPD
                     STSASGAYKGATDYRIGAQDLLAISVFGVQELNKEVRVNSNGQISLPLIGGVMAGGRT
                     IPELEDDLAKKYSTG"
     gene            502..576
                     /locus_tag="PK399_12700"
     tRNA            502..576
                     /locus_tag="PK399_12700"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:534..536,aa:Val,seq:cac)
     gene            781..1443
                     /locus_tag="PK399_12705"
     CDS             781..1443
                     /locus_tag="PK399_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPW14021.1"
                     /translation="MPGYRVREIEHAIGSHAYRLRVLVDTQQFADPDGHSARIGISSA
                     QWSLFGQVWPAGRLLAQAMHRFDIDGKRIMELGCGIGLASLVLKRRGADVVATDVHPL
                     AEVFLAYNAALNDLSALHYRQLRWHQPLPALGRFDVIIASDVLYERDHAELIAGVVER
                     HALPIAEVLVTDPGRGNSARFSRLLAAQGFEVDAEHCPMDDADRPPHRGRMLHYRRGN
                     LQ"
     gene            1479..1760
                     /locus_tag="PK399_12710"
     CDS             1479..1760
                     /locus_tag="PK399_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPW14022.1"
                     /translation="MRCDAVCCRLTVVLQSEDRIPPRFTTLTEAGLHVMDRDEEGWCV
                     AIDGARMQCSIYEIRPQICRRFVMAGPYCREVRADYSDRNARGIPLQLY"
     gene            1773..2042
                     /locus_tag="PK399_12715"
     CDS             1773..2042
                     /locus_tag="PK399_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14023.1"
                     /translation="MPSPKKPHPAGAAPPAIAPSKTSHLFAPRKANDRTPVTREAIEA
                     DMEAFQRAGGKIEVLGITRALHRIDADPSGAPPAAPAKPTSSRSR"
     gene            complement(2073..>2862)
                     /gene="cysC"
                     /locus_tag="PK399_12720"
     CDS             complement(2073..>2862)
                     /gene="cysC"
                     /locus_tag="PK399_12720"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HPW14024.1"
                     /translation="KHKVDVNTQEFLAAKHLELNEVAYCNLHLDQPVPFAAYAQNRSL
                     GGFILIDRQSNATVAAGTLDFALRRAGNIHWQHMDVDKSSRAAIKRQQPRCLWFTGLS
                     GAGKSTIANLVEKKLLAMGRHTYLLDGDNVRHGLNKDLGFTDEDRVENIRRVAEVAKL
                     MTDAGLVVLVSFISPFRAEREMARALFEPGEFLEVHVDTTLAEAERRDVKGLYAKARR
                     GELRNFTGIDSPYEAPEAPELHLDTAAADPATLADQVVRALDLD"
BASE COUNT          467 a          982 c          937 g          476 t
ORIGIN      
        1 tagccggtgg aatacttctt cgccaggtcg tcctcgagtt cggggatggt ccttccaccg
       61 gccatcaccc caccgatcaa cggcagcgag atctggccgt tggaattgac ccggacttcc
      121 ttgttcaact cctggacccc gaagacgctg atcgccagca ggtcctgcgc gccgatgcgg
      181 tagtcggtgg cacccttgta ggcacccgat gcggaggtcg agtccggcgc cggaagcggc
      241 tcggccgaaa cgttggcgac ccgttgccca ccgccactcg cgcaagcggc gagcagcagc
      301 aagagcaggc aggcaaggtg caggcgcacg gcgcgcacga tcatcgaagc catcgtcaaa
      361 atgtgtccgg agttgcagaa caagccgcca tcttaggcgg gatgcggcaa ggatgcgcgc
      421 ctggggcgtg acgggagcgg gaaaagccgc ttcccggccg ttgccggggc gctccgttca
      481 tgtacaatgc gcggcctctt cgggcggtta gctcagcggt agagcattgc cttcacacgg
      541 caagggtcgc aggttcgaac cctgcaccgc ccaccagttt gcgaagacgc cggccttgcg
      601 ccggcgtttt tgttccagcc aggctgccag tgagggccgc catcgccgca ctggcgtacg
      661 atggctgctt gacagcccca tatcttgcgt cccgcccgac cgatcacacc gtcgtccgct
      721 gcgcttctct tccatgcgtc aaatgccccg tcacggggtg acgccctacc gggagcgcca
      781 atgccggggt accgggtccg cgaaatcgaa cacgccatcg gcagccatgc ctatcgcctg
      841 cgtgtgctcg tcgataccca gcagttcgcc gatccggatg gccacagcgc ccgcatcggc
      901 atctcctcgg cgcaatggag cctgttcggc caggtctggc ccgccgggcg cctgttggcg
      961 caggcgatgc atcgcttcga catcgacggc aagcgcatca tggagctcgg ttgcgggatc
     1021 ggcctggcca gcctggtcct gaagcggcgc ggcgcggacg tggtcgccac cgacgtgcat
     1081 ccgcttgcgg aagtgttcct ggcctacaac gcggcgctga acgacctgtc cgccctgcac
     1141 taccgccagt tgcgctggca ccagccgctg cctgcactgg gccgcttcga cgtgatcatc
     1201 gccagcgacg tgctctacga gcgcgaccac gccgaactca tcgccggggt ggtggaacgc
     1261 cacgcgctgc ccatcgccga ggtcctggtc accgatccgg gccgcggcaa cagcgcgcgc
     1321 ttcagccgcc tgctcgcggc gcaagggttc gaggtggatg ccgagcattg cccgatggac
     1381 gatgccgacc gcccccccca tcgcggcagg atgctgcact accgtcgcgg aaacctgcag
     1441 tgaacgcgca ggtcgcgcca tccagcgacg ccgactgcat gcgctgcgat gcggtctgtt
     1501 gccgcttgac cgtggtcctg cagtccgagg accgcattcc gccgcgtttc accacgctca
     1561 cagaggcggg cctgcacgtg atggaccgcg acgaggaagg ctggtgcgtg gcgatcgatg
     1621 gcgcgcgcat gcagtgttcg atctacgaga tacgcccaca gatctgccga cgcttcgtca
     1681 tggccggccc gtattgccgc gaggtgcgcg ccgattacag cgatcgcaac gcccgcggca
     1741 tcccgctaca gctctactag gagacaggca tcatgccaag cccgaagaaa ccgcaccctg
     1801 caggtgccgc accgcccgcg atcgcaccgt cgaagaccag ccacctgttc gccccgcgca
     1861 aggccaacga ccgcaccccg gtgacgcgcg aggcgatcga agccgacatg gaggcgttcc
     1921 agcgcgccgg cggcaagatc gaggtgctcg ggatcacccg cgcactgcat cgcatcgatg
     1981 ccgatccctc cggcgcgcca cccgcggcgc cggccaagcc gacctcgtcg cgcagccgct
     2041 gaaccccgac gccgccagcg cgccggtggc ggtcagtcca ggtcgagcgc acgcacgacc
     2101 tggtcagcca gcgtcgccgg gtcggcggcg gcggtgtcca ggtgcaattc cggcgcttcc
     2161 ggcgcttcgt agggcgagtc gatgccggtg aaattgcgta gctcgccccg gcgtgccttg
     2221 gcgtagaggc ccttgacgtc gcgccgctca gcttcagcca gagtggtgtc gacgtgcact
     2281 tccaggaact cgccgggttc gaacagcgcg cgcgccatct cgcgctcggc gcggaacggg
     2341 ctgatgaagc tgaccaggac gaccaggccg gcgtcggtca tcagcttcgc gacttcggcg
     2401 acgcggcgga tgttctcgac ccggtcttcg tcggtgaagc ccaggtcctt gttgagcccg
     2461 tggcggacgt tgtcgccatc cagcaggtag gtgtgccggc ccatcgccag cagtttcttc
     2521 tcgacgaggt tggcgatcgt cgacttgccg gcgcccgaca ggccggtgaa ccacaggcag
     2581 cgcggctgct ggcgcttgat cgcggctcgt gaactcttgt cgacatccat gtgctgccaa
     2641 tggatgttgc cggcgcgacg cagggcgaaa tccagggtgc cggcagcgac cgtggcattg
     2701 ctctgccgat cgatcaggat gaacccaccc aggctgcgat tctgcgcgta tgcggcgaac
     2761 ggcaccggtt gatcgagatg caggttgcag taagcgacct cgttcaattc cagatgcttg
     2821 gcggccagga actcctgggt gttgacgtcg accttgtgct tg
//
LOCUS       DAOOCX010000222         2822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_35359_length_2822_cov_10.707626, whole genome shotgun
            sequence.
ACCESSION   DAOOCX010000222 DAOOCX010000000
VERSION     DAOOCX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35359_length_2822_cov_10.707626"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="PK399_12725"
     CDS             <1..144
                     /locus_tag="PK399_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPW14025.1"
                     /translation="AYKAAAASADRAGQGRTPEGSLAVRTSPGGAADLRLDTLRARFD
                     ALS"
     gene            148..1284
                     /gene="proB"
                     /locus_tag="PK399_12730"
     CDS             148..1284
                     /gene="proB"
                     /locus_tag="PK399_12730"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPW14026.1"
                     /translation="MNAFAPQPLPNWRRAVLKVGSSLLAGDGGLDPVHARGLAGFIAA
                     SRLQGREVVLVSSGAVAAGRGRIGTAGNGIVQRQALAALGQASLMGFWQALFDAPVAQ
                     VLLTHDDLRNRRRYLNARTALQELLRLGAQPIVNENDTVAVDELKLGDNDNLAAAVAS
                     LVEADLLLIATDIAGLYSGHPQGDADARPIERVDVVTPAVLAMAGGAGALGTGGMRTK
                     LEAASKASAAGIATVLFCGRDAATVDALGRDVLHGTFIAAHGSRISARKQWLRHSPAS
                     GALRVDDGAKAALLRGASLLPGGVQDVEGDFRRGDVVDVHAGDGTLLARGQAQYNAGE
                     SARIAGRHSRDIEAILGFRYDEAMIHRDDLVLLQHIDTIKEATA"
     gene            1281..2558
                     /locus_tag="PK399_12735"
     CDS             1281..2558
                     /locus_tag="PK399_12735"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPW14027.1"
                     /translation="MTAAFTGGYVRGLALAAREAQVAIATATADTRRDLLVAMADHLQ
                     AGQEAILAANAGDLAKAAANGATKATLDRLALEPERVRGMADALREVAAQPDPLGEIT
                     RRDTRPNGLVIERQRIPLGLVAMIYEARPNVTAEAAALCLKAGNAVLLRGGSEAAASN
                     AAIAACLHAALREAGLPEAAVSLVADTAREHVLELLQLSDLIDLAIPRGGESLIRFVA
                     EHARVPVIKHYKGVCHLYVDRAADIDLALGLLVDGKTSRPGVCNALETLLVHRDIAAE
                     FLPRALAVLAAHGVEVRGDEATRGFAPTLAPATDADYDAEFLDLVIAVKVVENLQAAL
                     AHIARFGSDHTEVIATTDDAAARAFVRGTRSSAVMVNASSRFNDGGQLGLGAEIGIST
                     TRLHAYGPMGVEALTIERFVVRGEGQRRHAMIG"
BASE COUNT          419 a          944 c         1002 g          457 t
ORIGIN      
        1 gcctacaagg ccgcggccgc ctcggctgat cgcgcggggc aggggcgcac gcccgaaggc
       61 tcgctggccg tgcgcacctc gcccggtggc gcggcagatt tgcgcctcga cacgttgcgc
      121 gcgcgcttcg acgcattgag ctgaacgatg aatgccttcg caccgcaacc cctgccgaac
      181 tggcgacgcg cggtgctgaa ggtcggcagc agcctgctcg ctggcgatgg cgggctggat
      241 ccggtgcatg cgcgagggct ggccgggttc atcgccgcgt cgcgcttaca gggccgagaa
      301 gtcgtcctgg tctcgtccgg tgccgttgcc gcaggtcgcg ggcgcatcgg cacggccggc
      361 aatggcatcg tccagcggca agccttggcc gcgctcgggc aggcctcgct gatgggcttc
      421 tggcaggcct tgttcgatgc gcctgtcgcg caggtgttgc tcacccatga cgacctgcgc
      481 aaccggcgtc gctatctcaa cgcgcgcacc gcgctgcagg aattgctgcg gcttggcgcg
      541 cagccgatcg tcaacgagaa cgacaccgtc gcggtggacg aactcaagct gggcgacaac
      601 gacaaccttg ccgctgcggt cgcatcgctg gtcgaagccg acctgctgct gatcgcgacc
      661 gacatcgccg gcctgtacag cgggcatccg caaggcgatg ccgatgcgcg tccgatcgaa
      721 cgcgtggacg tggtcacccc ggccgtgctg gcgatggccg gcggcgccgg cgcgctcggc
      781 actggcggga tgcgcaccaa gctggaagcg gcgtcgaagg cctcggcggc cggcatcgcc
      841 acggtgctgt tctgcggccg cgacgcggcg acggtcgatg cgctgggacg cgatgtactg
      901 catggcacct tcatcgccgc gcatggcagc cggatcagcg cacgcaagca atggttgcgg
      961 cattcgcccg catccggcgc gctccgcgtg gatgacggcg cgaaggcggc gctgctgcga
     1021 ggcgcgtcgc tgctccccgg tggcgtgcag gatgtcgaag gcgatttccg tcgtggcgac
     1081 gtggtcgacg tgcatgcggg cgacggcacg ttgctggcgc gcgggcaggc gcaatacaac
     1141 gccggcgaaa gcgcgcgcat cgccggtcgc cacagccgcg acatcgaggc gatcctcggt
     1201 ttccgctacg acgaggcgat gatccatcgc gatgatctgg tgctgctgca acacatcgac
     1261 acgatcaagg aagcgacggc atgacggcgg cttttaccgg tggttatgtg cgcggactgg
     1321 cgctggcggc gcgcgaggcg caggtggcga ttgcaaccgc aaccgccgac acccgtcgcg
     1381 acctgttggt agcgatggcc gaccacttgc aggcggggca agaggcgatc ctcgccgcga
     1441 acgccggcga tctcgccaag gccgctgcca atggtgcgac caaggcgact ctggaccggc
     1501 ttgcgctgga acccgagcgc gtgcgcggca tggcagacgc gctgcgcgaa gtcgcggcgc
     1561 agcctgatcc gctcggcgag atcacccgcc gcgacacgcg tccgaacggg ctcgtcatcg
     1621 aacgccagcg tattccgctg ggcctggtgg cgatgatcta cgaagcacgc ccgaacgtga
     1681 ccgccgaggc cgcggcgctg tgcctgaaag ccggcaatgc ggtgctgctg cgtggcggtt
     1741 cggaagcggc cgccagcaat gccgcgatcg cggcttgcct gcatgcggca ttgcgcgagg
     1801 ccggcttgcc tgaagccgcc gtctccctgg tcgccgacac cgcgcgcgag cacgtgctgg
     1861 agttgctgca gctctccgat ctcatcgacc tggccatccc gcgtggcggc gagagcctga
     1921 tccgcttcgt cgccgaacac gcccgagtcc cggtgatcaa gcattacaag ggcgtctgcc
     1981 atctctacgt cgatcgcgcc gccgacatcg accttgcgct tggcctgctg gtcgatggca
     2041 agacgtcgcg tcccggcgtc tgcaatgcct tggagacgct gctggtccat cgcgacatcg
     2101 ccgcggagtt cctgccgcgt gcgctggctg tgctggcagc gcatggcgtg gaggtgcgcg
     2161 gggacgaggc gacacgtggc ttcgctccca cgctcgcacc cgcgacagat gccgactacg
     2221 acgccgaatt cctcgacctc gtcatcgcgg tgaaggtggt cgaaaacctg caggccgcac
     2281 tggcgcatat cgcccgtttc ggttccgacc acaccgaagt gatcgccacc acggatgacg
     2341 cggcggcacg ggccttcgtg cgcggcaccc gcagttcggc ggtgatggtg aatgcgtcct
     2401 cgcgcttcaa cgacggcggc cagctgggcc tcggcgccga gatcggcatc tcgaccacgc
     2461 gcctgcatgc ctacggcccg atgggggtag aggcgctgac catcgaacgc ttcgtggtgc
     2521 gtggcgaggg gcagcgtcgc cacgcgatga tcggctgacg ccggccacgc caccgtcgac
     2581 gcggatggtg gccacggcgt gagccgattc gcccctgctt tgcgccccta ctggcgtggg
     2641 tggtgctgaa ttcccgttcg ttcggtggag ccgactaggc cttttggtcg ataggcgatc
     2701 ggacagcgcc agcactaccc tgcgggtggc cgccgggtcg ccggggctgt tatcgaacgc
     2761 aggggacggg cgatggactc ttcattgcga tgcgcaaggc atcgtgcgtt gtcgttgctg
     2821 gt
//
LOCUS       DAOOCX010000223         2793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_35917_length_2793_cov_8.336377, whole genome shotgun sequence.
ACCESSION   DAOOCX010000223 DAOOCX010000000
VERSION     DAOOCX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35917_length_2793_cov_8.336377"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PK399_12740"
     CDS             complement(<1..1060)
                     /locus_tag="PK399_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPW14028.1"
                     /translation="MFEWWFRIKFWKRVVAGFVLGALAGWAFGADAETWFGPLGDLYV
                     TLIKMIAVPLVFFAVINAVASLSGQKSIAALAGRTFLWFAITAVLAVGVGLAFGWLLK
                     PGVGVGVLQVASDYSPKNVPGVLDMLLNLVPSNPFQALGGASTAKTADGMTVLVPRNG
                     TVLQVIFFAGLVGFAIVKLGEKVAGMRKLVGEASEIMIQVTRFVLEFTPIGTFGLIAA
                     LVGGYGFEQLRPLLMFVIALYAACAFHIVVVYSGLLLAHGLNPLKFFRGAAPGMQVAF
                     VASSSFAALPVSLRSATHNLGVDKDYAAFAVPLGATIKMDGCGAIYPALAAVFISQYA
                     GIELSLSQYLIIALASVLG"
     gene            1175..>2793
                     /locus_tag="PK399_12745"
     CDS             1175..>2793
                     /locus_tag="PK399_12745"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPW14029.1"
                     /translation="MTPFPRRRPWLPFACVFALTACASASTQRPSTQALSTPAAGIAV
                     AVPAIAHPGGETAAWWFRDGASQAAARGAMQGRAKNVIIFLGDGMSLTTVAAARIFEG
                     QRKGGSGEENRLAWETYPATALSKTYNTDSQTPDSAGTMSAIATGVKTRAGVLSIAQS
                     AARKDCAAALAAPALTLWELASNSGMATGVVTTARVTHATPGATFTHSPERNWENDSD
                     LPAQAVREGCIDIARQLVETPFGRGFDVLMGGGRKNFMPVTQRDPEEDDKVGERLDGR
                     DLIADWKQRHAGGVYVWNKAQLDAAPLDKPLLALFQPDHMQFEYERPKDTAGEPTLAE
                     MTRAAIARLSRNGNGYVLLVEAGRIDHANHYGNAYRALSDTVAMSDAVRAANEATSVD
                     DTLIIVTADHSHVLNFAGYPQRGNPILGKVRGGSGEDDDGGLVNDGLGLPYTTLTYAN
                     GPGYTGASSSQPAGPKRYPHMGKEYEAAHGRPDLRNVDTEDPDYMQESTVPLTNETHG
                     GEDVGIWARGPGSDAIRGSVEQNAIFHFAAQA"
BASE COUNT          539 a         1022 c          899 g          333 t
ORIGIN      
        1 tgcccagcac cgaggccagc gcgatgatca ggtactgcga cagcgacagt tcgatgccgg
       61 cgtactgcga aatgaagacc gccgccagcg ccgggtagat cgcgccgcag ccatccatct
      121 tgatggtcgc gcccagcggc accgcgaacg cggcgtaatc cttgtcgacg ccgaggttgt
      181 gggtggcgct gcgcaacgac accggcaagg ccgcgaagct gctcgacgca acgaacgcca
      241 cctgcatgcc gggcgccgcg ccgcggaaga acttcagcgg gttgagtcca tgcgcgagca
      301 gcaggccgct gtagaccacg acgatgtgga aggcgcaagc cgcatacagc gcgatgacga
      361 acatcagcag cgggcgcaac tgctcgaacc cgtagccgcc gaccagcgcc gcgatcaggc
      421 cgaacgtgcc gatcggggtg aactccagca cgaaccgggt gacctggatc atgatctcgc
      481 tggcctcgcc gaccagcttg cgcatgcccg cgaccttctc gcccagcttg acgatggcga
      541 agccgaccag gccggcgaag aagatcacct gcagcaccgt gccgttgcgc ggcaccagca
      601 cggtcatgcc gtccgcggtc ttggcggtgg acgcgccacc cagggcctgg aagggattgg
      661 acggcaccag gttgagcagc atgtccagca cgccggggac gttcttcggc gaatagtcgg
      721 acgcgacctg caacacgccc acgcccacgc cgggcttgag cagccagccg aacgccaggc
      781 ccacgccgac cgccagcacg gcagtgatcg cgaaccacag gaaggtgcgc ccggcgagcg
      841 cggcgatcga cttctgcccg ctcagcgagg ccaccgcatt gatcaccgcg aagaagacca
      901 ggggcaccgc gatcatcttg atcagggtga cgtagagatc gcccagcgga ccgaaccagg
      961 tttcggcgtc ggcaccgaac gcccaaccgg ccaacgcccc gagcacgaag ccggcgacca
     1021 cgcgcttcca gaacttgatg cggaaccacc actcgaacat gcgggtcacc cggcaacggc
     1081 agaccgcgac catacccgag gccccacggg tccgtcagtg ccctgcctgc aacgctgaag
     1141 gacgagcgac atgcggaacg ttcataatcg acggatgacg ccattccccc gacgccgtcc
     1201 ctggcttccg ttcgcctgcg tattcgccct caccgcctgc gccagcgcct ccacccaacg
     1261 cccatcgacg caggcgctgt cgacgcccgc agccggcatc gcggtcgccg tgccggcgat
     1321 cgcccatccc ggcggggaaa ccgccgcgtg gtggttccgc gacggcgcat cgcaggccgc
     1381 ggcgcgcggc gcgatgcagg gccgggcgaa gaacgtgatc atcttcctgg gcgatggcat
     1441 gagcctgacc acggtcgccg ccgcacgcat cttcgaaggc cagcgcaagg gcggcagtgg
     1501 cgaggaaaac cgcctggcct gggagaccta tcccgcgacc gcactgagca agacctacaa
     1561 caccgactcg cagacgccgg attcggccgg caccatgagc gcgatcgcca ccggggtgaa
     1621 gacccgcgcc ggcgtgctga gcatcgcgca gtccgcggcg cgcaaggact gcgcagccgc
     1681 gctggcggca cccgcgctga ccctgtggga acttgcgtcg aacagcggca tggccaccgg
     1741 cgtggtcacc accgcgcgcg tcacccatgc cacgcccggc gccaccttca cccattcgcc
     1801 ggaacgcaac tgggagaacg acagcgacct gcccgcgcag gccgtgcgcg aaggctgcat
     1861 cgacatcgcc cggcaactgg tggaaacccc gttcggacgc ggcttcgacg tgctgatggg
     1921 cggcggccgc aagaatttca tgccggtgac ccagcgcgat ccggaagagg acgacaaggt
     1981 cggcgagcgc ctcgacggcc gcgacctcat cgccgactgg aagcagcgcc atgccggcgg
     2041 cgtctatgtc tggaacaagg cccagctcga cgccgcgccg ctcgacaagc cgctgctcgc
     2101 gctgttccag cccgaccaca tgcagttcga atacgaacgc ccgaaggaca cggcaggcga
     2161 accgacgctg gcggagatga cccgcgcggc gatcgcgcgc ctgtcgcgca acggcaacgg
     2221 ctacgtcctg ctggtcgaag ccggccgcat cgaccacgcc aaccactacg gcaacgccta
     2281 tcgcgcgctc agcgacaccg tggcgatgag cgacgccgtg cgcgcggcga acgaggcgac
     2341 ctccgtcgac gacaccctga tcatcgtcac cgccgaccac tcccacgtgt tgaatttcgc
     2401 cggctacccg cagcgcggca acccgatcct gggcaaggtg cgcggcggca gcggcgagga
     2461 cgacgacggc gggctggtca acgacggcct gggcctgccc tacaccaccc tcacctacgc
     2521 caacggcccc ggctacaccg gcgccagcag ctcgcagccc gccggcccga agcgttatcc
     2581 gcacatgggc aaggaatacg aggccgcgca tggccgcccc gacctgcgca acgtggatac
     2641 cgaagacccc gactacatgc aggaatccac cgtcccgctg accaatgaaa cccacggtgg
     2701 cgaggacgtc ggcatctggg cacgcgggcc cggcagcgac gcgatccgcg gcagcgtgga
     2761 gcagaacgcg atcttccatt tcgccgcgca agc
//
LOCUS       DAOOCX010000224         2696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_37927_length_2696_cov_7.032185, whole genome shotgun sequence.
ACCESSION   DAOOCX010000224 DAOOCX010000000
VERSION     DAOOCX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37927_length_2696_cov_7.032185"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="PK399_12750"
     CDS             complement(<1..1347)
                     /locus_tag="PK399_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPW14030.1"
                     /translation="MSNSRSRALHAARSPLTLALALALSSPAIAADPAIETTRADDPS
                     IAADPQRKPKDLDSVEVVADHLDKPSSSKYTEPLLDTPQTITVVTKTVMDQQNLIGLR
                     DVLSTLPGITFGAGEGGGGYGDSINLRGFSASNDITVDGVRDSAQYTRSDNFNLEALE
                     LVNGANSAMSGAGSVGGNINLVSKTAHAGNNHDFTLGAGTDGMARLTADSNMELSDGA
                     ALRLNAMVHQNDVPDRDVERYERWGFASSFVMGLGSDTRFSVNVLHQQDDNIPEYGLP
                     YFASYGGLLPGVDRESYFGYANIDTQEIEATTLTGVLDHDFSDTASLRTLARVQRVDQ
                     LSIVNPTQGTWCLDSGVNPATGAACTDVGTYLPSGPRGTSRDTRNGLAVLQSDLTVRF
                     ATGAVEHALVAGVSLASETYSLDNGNVQRNANGTTPTYPPMDLQDPDKLYTGPIHYI"
     gene            complement(1436..1774)
                     /locus_tag="PK399_12755"
     CDS             complement(1436..1774)
                     /locus_tag="PK399_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HPW14031.1"
                     /translation="MSEAWTYVCSTAELLPGEMKVVFDEVTQAAIAVFNLDGMLYALE
                     DRCSHEDFELSAGHFDAAEGSVECVLHGARFDIRDGRALCAPAYEPVAKFPVRREDDR
                     ILTRDDREDS"
     gene            complement(1771..2295)
                     /locus_tag="PK399_12760"
     CDS             complement(1771..2295)
                     /locus_tag="PK399_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW14032.1"
                     /translation="MTDLHFRAARLADLDAIVDLVTSAYRGDASRAGWTTEADFLDGN
                     RIDAEVLRADIERPRSRVLLAERDGQLLACAHVSEDDGAGYFGMFSVVPGLQGGGIGK
                     QVLAECERIAREDWRLPAMRMTVIDIRDELIAFYERRGYRRTGILKPFPYGDERFGLP
                     QRDDLRFEVLEKTL"
     gene            complement(2305..2562)
                     /locus_tag="PK399_12765"
     CDS             complement(2305..2562)
                     /locus_tag="PK399_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14033.1"
                     /translation="MDHDPQIAQLLREIRDHQREALALQREHIAAFKAQVERVERFNE
                     RAESLQGRAGKAVKALLWVVLPLLALVLLLMAWPYARYMFG"
BASE COUNT          405 a          856 c          956 g          479 t
ORIGIN      
        1 gatgtagtgg atcgggccgg tatagagctt gtccgggtcc tgcaggtcca tgggcgggta
       61 cgtcggcgtg gtgccgttgg cgttgcgctg cacgttgccg ttgtcgagcg aataggtctc
      121 gctggccagc gacacgccgg cgaccaaggc gtgttccacc gcacccgtgg cgaaacgcac
      181 ggtcaggtcg ctctgcagca ccgccaggcc gttgcgggtg tcgcggctgg tgccgcgcgg
      241 gccgctgggc aggtaggtgc cgacgtcggt gcaggccgcg ccggtggccg gattgacgcc
      301 gctgtccagg caccaggtgc cctgggtcgg gttgacgatg ctgagctggt cgacgcgctg
      361 cacgcgcgcc agggtgcgca ggctggccgt gtcgctgaag tcgtggtcga gcacgccggt
      421 cagggtggtc gcctcgatct cctgggtgtc gatgttggcg tagccgaaat agctttcgcg
      481 atcgacgccc ggcagcaggc cgccgtacga cgcgaaatac ggcaggccgt attcggggat
      541 gttgtcgtcc tgctggtgca ggacgttgac gctgaagcgg gtgtcggaac cgaggcccat
      601 cacgaacgag gacgcgaagc cccagcgttc gtaccgctcg acatcgcggt ccgggacatc
      661 gttctggtgg accattgcgt tgagccgcag cgcggcgcca tcggacagtt ccatgttgct
      721 gtcggcggtc aggcgggcca tgccatcggt gcccgcgccc agggtgaagt catggttgtt
      781 gccggcatgc gcggtcttgc tgaccaggtt gatgttgccg ccgaccgaac ccgcgcccga
      841 catcgccgag ttggcgccgt tcaccagctc cagcgcttcc aggttgaagt tgtcgctgcg
      901 ggtgtactgc gcgctgtcgc gcacgccatc cacggtgatg tcgttgctgg cgctgaaacc
      961 gcgcaggttg atgctgtcgc cgtagccgcc gccgccttcg ccggccccga aggtgatgcc
     1021 gggcagggtg ctgaggacgt cgcgcagccc gatcaggttc tgctggtcca tcaccgtctt
     1081 ggtgaccacg gtgatcgtct gcggggtgtc cagcagcggc tcggtgtact tgctcgatga
     1141 gggcttgtcg agatggtcgg cgaccacttc caccgaatcc aggtccttcg gcttccgctg
     1201 cggatccgcc gcgatgctgg ggtcgtcggc gcgggtggtc tcgatggcag gatccgcggc
     1261 gatcgccggg ctggacagcg ccagggccag ggccagggtc agcggcgatc gggccgcgtg
     1321 cagggcgcgc gagcgggaat tcgacatggg ggtcctcgtt gcatggcgtg cggcgcttgg
     1381 gtggccgctt cagtcgagca tgctaatgag aaccattatc aaatgcaatc agatatcaac
     1441 tgtcttcgcg gtcgtcccgg gtcaggatcc tgtcgtcctc gcgacgtacc gggaatttcg
     1501 caaccggttc gtatgccggc gcgcacaggg cgcggccatc gcggatatcg aagcgggcgc
     1561 catgcagcac gcattccacg ctgccttctg ccgcatcgaa atggccggcc gacaactcga
     1621 agtcctcgtg cgaacagcgg tcttcaagcg cgtacagcat cccgtccagg ttgaacaccg
     1681 cgatcgcggc ctgggtcacc tcgtcgaaga cgaccttcat ctcgcctggc aacagctcgg
     1741 cggtgctgca gacgtaggtc caggcctcgc tcacagggtc ttctccagca cttcgaagcg
     1801 caggtcgtcg cgctgcggca ggccgaagcg ctcgtcgccg tacgggaacg gcttgaggat
     1861 gccggtgcgc cgatatccgc ggcgctcgta gaaggcgatc aattcgtcgc ggatgtcgat
     1921 caccgtcatc cgcatcgcgg gcaagcgcca gtcttcgcgg gcgatgcgtt cgcattcggc
     1981 cagcacctgc ttgccgatgc cgccgccctg cagcccgggc acgaccgaga acatgccgaa
     2041 gtagccggcg ccgtcgtctt cactgacgtg agcgcaggcc agcaactgcc cgtcgcgttc
     2101 cgcgagcaac acgcggctcc tggggcgttc gatgtcagcg cgcaggacct cggcatcgat
     2161 gcggttcccg tcgaggaaat cggcttcggt ggtccagccg gcgcggctgg catcgccacg
     2221 ataggcggag gtgaccaggt cgacgatggc gtcgaggtcg gccagcctgg cggcgcggaa
     2281 gtgcaagtca gtcatgggtg ggcatcatcc gaacatgtag cgggcgtagg gccaggccat
     2341 cagcagcagg accagcgcga gcaacggcag cacgacccac agcaacgcct tcaccgcctt
     2401 gccggcgcgc ccctgcagcg attcggcgcg ttcgttgaag cgctccacgc gttccacctg
     2461 cgccttgaac gcggcgatgt gctcgcgttg cagcgccagc gcttcgcgtt ggtggtcgcg
     2521 gatctcgcgc aagagttggg cgatctgcgg gtcgtggtcc atcgcggcct ccgggtcggc
     2581 ctgagtctag gcgagcaggc ggcggacctt gcgcaacgcg gcgacgaagg cgtcgacctc
     2641 gtcgtgcgtg ttgtagaacg ccagcgacgc gcggcaggtc gcggccacgc cgaacc
//
LOCUS       DAOOCX010000225         2678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_38318_length_2678_cov_7.565002, whole genome shotgun sequence.
ACCESSION   DAOOCX010000225 DAOOCX010000000
VERSION     DAOOCX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38318_length_2678_cov_7.565002"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PK399_12770"
     CDS             <1..750
                     /locus_tag="PK399_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HPW14034.1"
                     /translation="DKYRVEPPFKLQGSYRNMNKLAEKISPVMNDAELRQVIADHYLG
                     ESHLLTQGAEENLLKLGELLGRMTPEQQSRWAQIKADFLRNKAMGADDADVGGRMVAQ
                     LADIAGGLQGLRDEPPPPAPQPPPWSDLLDALARIATPPASPTAASAPEQPVGDLLRS
                     AHQPLLEGLERTLARQDHLHSALLELVEALKAGGITHASQREPAERVIPRIRTPEERA
                     FEKTISALKFKAAEDAKKADAAKPPGEPGAS"
     gene            747..1433
                     /locus_tag="PK399_12775"
     CDS             747..1433
                     /locus_tag="PK399_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14035.1"
                     /translation="MSELHMPRDEASLLDAARRAQAEAASLQSPAFDIQEALRNLRTV
                     LLSTDARALRKSVGFFGRLLGRDIELQAESAAMRGQLALLVLQARQRGEALDRHNHAL
                     ASLRDRLSTLANEFDAVIASLAATASDAATDHARLRLLESTRLGCALTATQLGVLVQN
                     GQALSARYQHMLPQVEGLLVQHRAALAGRDDAARMRDAAALVASVEVAIPASLSPPSE
                     IQPHAAQESP"
     gene            1430..2539
                     /locus_tag="PK399_12780"
     CDS             1430..2539
                     /locus_tag="PK399_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006381259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HPW14036.1"
                     /translation="MTTPNPESASLLPAALDEKSLLDLGLTSADIGKIQETSRALQDI
                     TPGNLHGYGREAAGKTSSFSTQLLDKVRNADLDNSGDKLGEVVRIARSLNLDSFGERS
                     KLPILGPLIDKLKATRDDLVQKYSSTNSQIDQLMRDVGQTQATQQQRVREYDQMHAIV
                     REERQELGIHVAAGRVRIAELQQELAALAGQDDPQSRIQRAELDTALRLLDKRVSDLQ
                     VLQHAADQTLPMIRLIQANAIQLIEKFSAVRDITIPMWRNQFAIQLSLADQRNAVDLA
                     NAIDDASNELMRKNADLVHATSVGTAKANQRSVIDIETLRHVHDKLIQTVEEVRDIHR
                     EGMAKRQQIGGELAQMRDEVQKRMAQGASEALLTG"
BASE COUNT          516 a          965 c          845 g          352 t
ORIGIN      
        1 gacaagtacc gcgtggaacc gccgttcaag ctgcagggca gctaccgcaa catgaacaag
       61 ctggcggaga agatctcgcc ggtgatgaac gatgccgagc tccgccaggt catcgccgac
      121 cactatcttg gcgagtcgca tttgctcacc caaggcgcgg aagaaaacct gctcaagctg
      181 ggcgaactgc tgggccggat gaccccggaa cagcagtcac gctgggcgca gatcaaggcc
      241 gacttcctgc gcaacaaggc gatgggcgcg gacgatgccg atgtcggcgg gcgcatggtc
      301 gcgcaactgg ccgatatcgc cggcggcctg cagggcctgc gcgacgaacc gcccccgccc
      361 gcgccgcaac cgccaccctg gagcgacctg ctggacgcgc tggcgcgcat cgccacgcct
      421 ccggcctcgc ccaccgcagc gtcggcaccc gagcagcccg tcggcgacct gctgcgcagt
      481 gcccatcagc cgctgctcga gggcctggag cgcacgctgg cgcggcagga ccacctgcat
      541 tcggcgctgc tggaactggt ggaggcgctg aaggccggcg gcattaccca cgcatctcag
      601 cgcgaacctg cggagcgggt cattccacgc atccgcacgc cggaggaacg cgcgttcgag
      661 aagaccatca gcgcgttgaa gttcaaggcc gccgaggacg cgaagaaggc ggatgcggca
      721 aagccgccag gcgaacccgg cgcttcatga gcgagctcca catgccacgc gacgaagcca
      781 gcctgctcga tgccgcgcgc cgtgcgcagg ccgaggcggc ctcgctgcag tcgcctgcgt
      841 tcgacatcca ggaagccctc cgcaacctgc gcaccgtgtt gttgtcgacc gacgcgcgcg
      901 ccttgcgaaa gagcgttggc tttttcggtc gcctgctcgg tcgcgacatc gagctgcagg
      961 cagaatccgc cgccatgcgc ggacaactcg cgctgctggt gttgcaggcg cggcaacgcg
     1021 gcgaggcact cgaccgtcac aatcacgcgc tcgcatcgtt gcgcgatcgc ctgtcgaccc
     1081 tcgccaacga attcgatgcc gtcatcgcca gccttgcggc aaccgcatcc gatgctgcaa
     1141 ccgaccacgc acgcctgcgc ctgctcgaat ccacccgcct cggctgcgcg ctgacggcaa
     1201 cccagctcgg cgtgctggtg cagaatgggc aggcgctatc ggcccgctac cagcacatgc
     1261 tgccgcaggt ggaaggcctg ctggtccagc accgcgccgc attggcgggt cgggacgatg
     1321 ccgcccggat gcgcgatgcc gccgcgctgg tcgcctccgt cgaagtggcg atcccggcat
     1381 ccctatcacc gccatccgaa atccagccgc acgccgcaca ggagtcgcca tgaccacgcc
     1441 caaccccgaa tccgcctccc tgctcccggc cgcactcgac gagaaatcgc tgctcgacct
     1501 gggcctgacc agtgccgaca tcggcaagat ccaggaaacc tcgcgcgcgt tgcaggacat
     1561 cacccccggc aacctgcacg gctacggccg cgaggccgcg ggcaagacca gcagcttctc
     1621 gacccagttg ctggacaagg tgcgcaatgc cgacctggac aactccggcg acaagctggg
     1681 cgaagtggtg cggatcgcgc gcagcctgaa cctggattcg ttcggcgaac gctccaagct
     1741 gccgatcctc ggcccgctga tcgacaagct gaaggccaca cgcgacgacc tggtgcagaa
     1801 gtacagctcg accaacagcc agatcgacca gctgatgcgc gacgtcggcc agacccaggc
     1861 cacccagcag cagcgcgtgc gcgaatacga ccagatgcac gccatcgtcc gcgaggaacg
     1921 ccaggaactc gggatccacg tcgccgccgg ccgcgtgcgc atcgccgaac tgcagcagga
     1981 actggccgca ctggcggggc aggacgatcc gcagagccgg atccagcgtg ccgagctcga
     2041 caccgcgctg cgcctgctgg acaagcgcgt gtccgacctg caggtgctgc agcacgccgc
     2101 cgaccagacc ctgccgatga tccggctgat ccaggccaac gcgatccagc tgatcgagaa
     2161 attcagcgcg gtacgcgaca tcaccatccc gatgtggcgc aaccagttcg cgatccagtt
     2221 gtcgctggcc gaccagcgca acgcagtcga cctggccaac gcgatcgacg atgccagcaa
     2281 cgaactgatg cgcaagaacg ccgacctggt gcacgccacg tcggtgggca cggccaaggc
     2341 caaccagcgt tcggtcatcg acatcgagac cttgcgccac gtccacgaca agctgatcca
     2401 gacggtcgag gaagtgcgcg acatccatcg cgagggcatg gccaagcgcc agcagatcgg
     2461 cggcgagctg gcgcagatgc gcgatgaggt gcagaagcgc atggcgcagg gcgcatcgga
     2521 agccctgctg accggatagg cacgtcacgg gacccggaaa cgcggtgcgg cttataattc
     2581 cgcatccgcc gaagcgacat cccatgcccg acaacgcctc gccatccctg caccggctcg
     2641 acctgcccgc gatccaggcc ctcgccgcgc cggaaatg
//
LOCUS       DAOOCX010000226         2644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_39065_length_2644_cov_7.888374, whole genome shotgun sequence.
ACCESSION   DAOOCX010000226 DAOOCX010000000
VERSION     DAOOCX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39065_length_2644_cov_7.888374"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="PK399_12785"
     CDS             <1..1293
                     /locus_tag="PK399_12785"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HPW14037.1"
                     /translation="LVCIFLFGGSDSFNMLIPHDSAEYNAYSTSRGGVYDAATNPTGL
                     GYARDQLVTIADTDGKTWGLNPGCTAMKPLFEAGELAFLANVGTLVEPVTKTEVNNKL
                     KRLPAYLYSHNDQQKLWMRGQSSVNTAVGWGGALSDYMEASNTGLQSLSPSISIAGSN
                     LFQVGRMTLPFTLSSGGPPTLTRFRNDAGNADRIRYESLRALVDASYAPVLQDEYALV
                     GESAIELNGSLRTVLDPANGGDIATVFPAGGLASQLRMVARMIKASRTAAIGHRRQVY
                     YVSLGGFDTHENQMAANGHGALLKQLGDSLAAFREALAEIGALNATTTFTMSDFGRTL
                     NSNGNGTDHGWGGVQLIMGGAAANGGALRGRQVWGQYPVLELDGSQAVGRGRMVPTLS
                     VNQVGSTLAQWFGLPSSSVGSIFPGIENFSQQKLGFLG"
     gene            complement(1315..2145)
                     /gene="pyrF"
                     /locus_tag="PK399_12790"
     CDS             complement(1315..2145)
                     /gene="pyrF"
                     /locus_tag="PK399_12790"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPW14038.1"
                     /translation="MTFIEKLRNRWQSANSLLCVGLDPDPAKFPDAYVDGMSDDTGDA
                     LFSFCRDIVDATAEFACAFKPQIAYFAAHNGGESALQRLIAHINGAHPDVPVILDAKR
                     GDIGSTAEQYAVEAFERFGADAVTLNPYMGRDSAAPFLAYNDRGCVFLCHTSNPGARD
                     FQELLVDGAPLYQHVARTIAGEWNTDGNCALVVGATFPEELKVIRGIVGDMPLLIPGI
                     GAQGGDVEATVRNGRTADGTGLMINSSRGILYASGGAGYADAAAEAARELRDAINRYR
                     "
     gene            complement(2241..>2644)
                     /locus_tag="PK399_12795"
     CDS             complement(2241..>2644)
                     /locus_tag="PK399_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW14039.1"
                     /translation="EIQSRAYIGRFNFKGQDQQKLVGTLSGGERGRLHMAKTLLQGGN
                     VLLLDEPSNDLDIETLRALEDALLEFPGNTFVISHDRWFLDRIATHILAFEGDSHVEF
                     FQGNYREYEEDKKRRLGNDAGPHRLRFKALK"
BASE COUNT          424 a          859 c          914 g          447 t
ORIGIN      
        1 ctggtctgca tcttcctgtt cggcggcagc gattccttca acatgctgat cccgcacgac
       61 agcgccgagt acaacgccta ttcgaccagc cgtggcggcg tgtacgacgc cgcgaccaac
      121 ccgaccggcc tgggctatgc gcgcgaccaa ctggtcacca tcgccgacac cgatggcaag
      181 acctggggcc tcaatcctgg ctgcacggcg atgaagccgc tgttcgaggc cggcgaactc
      241 gccttcctgg ccaacgtcgg cacgctggtc gagccggtga ccaagaccga ggtcaacaac
      301 aagctcaagc ggttgcccgc ctatctgtac tcgcacaacg accagcagaa gctgtggatg
      361 cgcgggcagt cgtcggtgaa cactgcggtg ggatggggcg gcgcgctgtc cgactacatg
      421 gaggcgagca acaccggctt gcagtcgttg tcgccgtcta tttccatcgc cggcagcaac
      481 ctgttccagg tcgggcggat gaccttgccg ttcaccctgt cgtccggcgg gccgccgacc
      541 ctgacccgct tccgcaacga cgccggcaat gccgaccgca tccgctacga atccctgcgt
      601 gcgctcgtcg atgccagcta cgcccccgtg ctgcaggacg aatacgcgct ggtcggcgaa
      661 agcgccatcg aactcaacgg aagcctccgc acggtgctgg atccggcgaa tggcggcgac
      721 atcgccacgg tgtttccggc gggcgggctg gcgtcgcagt tgcgcatggt cgcgcgcatg
      781 atcaaggcca gccgcactgc ggcgatcggc catcggcggc aggtgtacta cgtcagcctg
      841 ggcggtttcg acacccacga aaaccagatg gccgccaacg gccacggtgc cttgctgaaa
      901 caactgggcg attcactcgc cgcgttccgc gaggcgctgg cggagatcgg cgcgctcaac
      961 gccaccacca ccttcaccat gagcgacttc ggacgcaccc tcaacagcaa tggcaatggc
     1021 accgaccacg gctggggcgg cgtgcaactg atcatgggcg gcgcggccgc gaatggcggc
     1081 gcgctgcgcg ggcgccaggt ctggggccag tacccggtgc tggaactgga tggcagccag
     1141 gcggtcgggc gcggccgcat ggtgccgacg ctgtcggtga accaggtggg ttcgacgctg
     1201 gcgcaatggt tcgggttgcc gtcgtcgagc gtgggctcga tcttccccgg catcgagaac
     1261 ttcagccagc agaagctcgg cttcctcggc tagcgcgagt cgcgatgcgt gcgatcaacg
     1321 gtaccggttg atcgcgtcgc ggagctcgcg tgcggcctcg gcggcggcgt cggcgtagcc
     1381 cgcgccgccc gaggcgtaga ggatcccgcg cgaggaattg atcatcaggc cggtgccgtc
     1441 ggcggtcctg ccgttgcgca cggtggcttc cacgtcgccg ccctgcgcgc cgatgcccgg
     1501 gatcagcagc ggcatgtcgc cgacgatgcc gcggatcacc ttcaactcct cggggaaggt
     1561 cgccccgacg acaagtgcgc agttgccgtc cgtgttccac tcgcccgcaa tcgtgcgggc
     1621 gacgtgctgg tacagcggcg cgccatcgac cagcagttcc tggaaatcgc gcgcgccggg
     1681 attgctggtg tggcagagga acacgcagcc gcggtcgttg taggccagga acggcgcggc
     1741 cgagtcgcgg cccatgtacg ggttgagcgt caccgcatcg gcgccgaagc gctcgaacgc
     1801 ttcgaccgcg tattgctccg cggtgctgcc gatgtcgccg cgcttggcgt ccaggatcac
     1861 cggcacgtcc gggtgtgcac cgttgatgtg cgcgatcagc cgctgcagcg cggattcgcc
     1921 gccgttatgc gcggcgaagt aggcgatctg cggcttgaac gcgcaggcga attcggcggt
     1981 ggcgtcgacg atgtcgcggc agaacgagaa cagggcgtcg ccggtgtcgt cggacatccc
     2041 gtcgacgtag gcatcgggga atttcgccgg atccggatcc aggccgacgc acagcagcga
     2101 gttcgccgac tgccagcggt tgcggagttt ttcgatgaag gtcatggcaa gcccgctcgt
     2161 cctgagcctg tcgaaggatg agcggggctg cgacggtccc cgcgctcgtg gttcgacagg
     2221 ctcaccacga gcggtgatgc ttacttcagt gccttgaacc gcagccggtg cgggccggcg
     2281 tcgttgccca ggcggcgctt cttgtcttcc tcgtattcac ggtagttgcc ctggaagaac
     2341 tccacgtgcg agtcgccctc gaaggcgagg atgtgggtgg cgatgcggtc caggaaccag
     2401 cggtcgtggg aaatgacgaa ggtgttgccg gggaactcca gcagcgcgtc ttcgagcgcg
     2461 cgcagggttt cgatgtcgag gtcgttggac ggttcgtcga gcagcagcac gttgccgccc
     2521 tgcagcaggg tcttggccat gtgcaggcgg ccgcgttcgc cgccggacag cgtgccgacc
     2581 agcttctgct ggtcctggcc cttgaagttg aagcggccga tgtaggcgcg cgactggatc
     2641 tcga
//
LOCUS       DAOOCX010000227         2640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_39156_length_2640_cov_9.455706, whole genome shotgun sequence.
ACCESSION   DAOOCX010000227 DAOOCX010000000
VERSION     DAOOCX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39156_length_2640_cov_9.455706"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1016
                     /locus_tag="PK399_12800"
     CDS             141..1016
                     /locus_tag="PK399_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HPW14040.1"
                     /translation="MANPRRFRILVALLCLGIASTAFAWGRLGHALVGDLAERHLDPT
                     AKAEVARLLAGEPDPTLAGVASWADDLRDSDPPRFKATSKWHYINAKGGGCGFDLARD
                     CPDGNCVVGAIQAQRAILADRSQPIQARRDALKFIVHFIGDAHQPMHAGSRTDSGGNG
                     FQVSLRTPIEPEAYNRNKYVDGVMGTNLHSIWDYYILAGRRLDLPAYAGKLDTLPWPP
                     FPAPSAHDVMPLAWAGESCRLIEARGIYPPEDKHTMDSAYLDAMRPLAEQRVRQAAWR
                     LAQLLNQTIGEQAAP"
     gene            1013..1762
                     /locus_tag="PK399_12805"
     CDS             1013..1762
                     /locus_tag="PK399_12805"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPW14041.1"
                     /translation="MSSTDLLLAALFLAVAVLYSSVGHAGASGYIASMALLGFAPEQV
                     RPTALALNLLVGGIGLFRWWRGGHVRWRNVLPFVLASAPAAFLSAQVKLPKESYSLLL
                     GVVLLAAAIGVFRHASRAEAEDEASRGRNVPWMAGLFIGAAIGTLSGLTGTGGAIFLT
                     PLLLFARWMPTRDASGVSVAFVWINSLTGLAGLLHSGQSLPAMLPWWLGVVAIGALIG
                     TQLGLKWLPVRGLRLALGVVLLIAAGKLLFA"
     gene            complement(1805..2203)
                     /locus_tag="PK399_12810"
     CDS             complement(1805..2203)
                     /locus_tag="PK399_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPW14042.1"
                     /translation="MSGLLAGCVSQLVAEPAREGYAADGAVNAEMVEQPDAARYQPVQ
                     GSAYLSPQPIRENARPEYPAGLLEQELPSVTLVARIVVSGAGALARAEVIERSHDQPA
                     FEDAVVAAIRTWVFIPQTNGRAVVEAGETR"
     gene            complement(2240..>2640)
                     /locus_tag="PK399_12815"
     CDS             complement(2240..>2640)
                     /locus_tag="PK399_12815"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW14043.1"
                     /translation="RMDIMLTDSAAQPVAVAPLSRQPHVYPVDVLENGVNGTVVLIVD
                     VAVDGSVSATKVDRSAGDERLDAAALEAVGQWKFKPLVKDGRPVPGKVRVPVEFRSGG
                     YLGDAVLEDGVIKTKVGDFRVVGSIASPKS"
BASE COUNT          406 a          956 c          825 g          453 t
ORIGIN      
        1 aggtcaagct caacggcgcc aacctcggta ccaggaacac cgtgaacatc gatccgtcgg
       61 tgtacggcat cgcctacgtg ttcgcgctct gacgcggggc tgtcataccg ccatcggcca
      121 tgccgggcta tcctgccggc atggccaatc cccgcagatt ccgcatcctc gtcgccctgc
      181 tctgcctggg catcgcctcc accgccttcg cctggggccg gctcggccat gcgctggtcg
      241 gcgaccttgc cgaacgccac cttgatccga ccgccaaggc cgaggtcgca cgcctgctgg
      301 ccggcgaacc ggatccgacg ctggccggcg tggcctcatg ggccgacgac ctgcgcgaca
      361 gcgacccgcc gcgcttcaag gctacgtcga agtggcacta catcaacgcc aagggcggcg
      421 gctgcggctt cgacctggcc cgcgactgcc cggacggcaa ctgcgtggtc ggcgcgatcc
      481 aagcccagcg cgcgatcctc gccgaccgca gccagccgat ccaagcacgc cgcgatgcac
      541 tgaagttcat cgtccatttc atcggcgacg cgcaccagcc gatgcatgcc ggcagccgca
      601 ccgacagcgg cggcaacggt ttccaggtca gcctgcgcac gccgatcgaa cccgaggcct
      661 acaaccgcaa caagtacgtc gatggcgtga tgggtaccaa cctgcactcg atctgggact
      721 actacatcct ggccggtcgc cgcctcgacc tgccggccta tgcgggcaag ctcgacacgc
      781 tgccgtggcc gccgttcccc gcaccgtccg cgcatgacgt gatgccgctg gcctgggccg
      841 gcgagtcctg ccgcctgatc gaggcgcgtg gcatctaccc gcccgaggac aagcacacga
      901 tggacagcgc ctacctggac gcgatgcgcc cgctggccga gcaacgcgtg cgccaggcgg
      961 cgtggcgatt ggcgcagctg ctgaaccaga ccatcggaga gcaggcggca ccgtgagcag
     1021 caccgacctg ttgctggccg cgctgttcct tgcggtcgcg gtgctgtatt cgtcggtcgg
     1081 gcatgccggc gcgagcggct acatcgcctc gatggcgctg ctcggcttcg cgccagaaca
     1141 ggtgcgcccc accgcgctgg cattgaacct gctggtgggc ggcatcggct tgttccgatg
     1201 gtggcgcggt ggccatgtgc gctggcgcaa cgtgctgccc ttcgtgctcg cctctgcacc
     1261 ggccgcgttc ctctctgcgc aagtgaaact tccgaaggaa agttattcgc tactgctcgg
     1321 cgtcgtgctg ctggccgccg ccattggcgt gttccggcat gcatcgcgcg ccgaagccga
     1381 ggacgaggcg tcgcgcggcc gcaacgtgcc gtggatggcc ggcctgttca tcggcgcggc
     1441 catcggcacg ctgtccggcc tgaccggcac cggcggcgcg atcttcctga ccccgctgtt
     1501 gctgttcgcg cgctggatgc cgacgcgcga tgccagtggc gtgtccgtgg ccttcgtctg
     1561 gatcaactcg ctgaccggac tggccggcct gctgcattcc ggccagtcac tgccggcgat
     1621 gctgccctgg tggctgggcg tggtcgcgat cggtgcgttg atcggcaccc agcttggcct
     1681 gaaatggctg ccggtgcgcg ggctgcgcct ggcgctgggc gtggtgttgc tgattgcggc
     1741 gggcaagttg ttgttcgcct gatgcaacca ctcgactgcc gatgacgagc agcgcgccac
     1801 atcgtcagcg ggtttcgccc gcctcgacca ccgcgcgtcc attcgtctgc gggatgaaga
     1861 cccacgtcct gatcgccgcc acaacggcgt cctcgaacgc cggttggtcg tgggaacgtt
     1921 cgatcacttc cgctcttgcc agcgcgcccg caccactcac aacgatgcgc gccaccagcg
     1981 tgaccgacgg caattcctgt tcgagcaatc cggcgggata ctcgggcctg gcgttctcgc
     2041 ggattggctg cgggctcaag taggcgctgc cttgcaccgg ctggtatcgc gcagcatcag
     2101 gctgctccac catttccgcg ttgaccgcgc catcggcggc gtagccctct cgtgctggtt
     2161 ccgccaccag ctgtgagacg cagccggcca acagtccgga caacgcaacc agtcgcagcg
     2221 ctgcagggag gtgcatacat caactcttcg gtgacgcaat gctgccgact accctgaaat
     2281 cacccacctt cgttttgatg acgccatcct ccagcaccgc atcgccgaga tagccgccag
     2341 accgaaactc gaccggcacg cgcaccttgc ctggcaccgg cctgccgtcc ttcaccagcg
     2401 gcttgaactt ccactgcccc acggcctcga gcgctgccgc gtccagccgt tcgtctcccg
     2461 ccgaacgatc caccttcgtc gccgacacac tgccatccac cgcaacatcg acgatcagca
     2521 cgacggtgcc gttcacacca ttctcgagaa catcgaccgg gtagacgtgc ggctgcctcg
     2581 aaagcggcgc gaccgcaacc ggttgcgcgg ccgaatcggt cagcatgatg tccatgcgca
//
LOCUS       DAOOCX010000228         2603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_40018_length_2603_cov_7.801805, whole genome shotgun sequence.
ACCESSION   DAOOCX010000228 DAOOCX010000000
VERSION     DAOOCX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40018_length_2603_cov_7.801805"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PK399_12820"
     CDS             complement(<1..969)
                     /locus_tag="PK399_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPW14044.1"
                     /translation="MSYSTQQIRNVALAGHPGAGKTTLFEALLHAGGAIQTAGSIERG
                     STVSDFDPIERERSHSIDAAIASIDHAPHGQPPIHVNLIDTPGYPDFRGPTLSALAAV
                     ETVAVVVDADTGISYGTQRMMAHAKARGLCRVIVVNKIDHPGAKLGALLDSLRETFGS
                     EVLPLNLPNEGGKSVVDCFWQSTGTSNLGPVADWHRRIIDQVVEVNETVMDHYLDLGE
                     GGLSGQELHDAFEQCLREGHLVPVCFVSARSGAGVRELLDVAERLFPHPGEANPPPFM
                     KGSGDEAQPIEATPDPQAHVIADVFKIINDPFVGKLGIFRVYQGTVR"
     gene            complement(1138..1872)
                     /locus_tag="PK399_12825"
     CDS             complement(1138..1872)
                     /locus_tag="PK399_12825"
                     /inference="COORDINATES: protein motif:HMM:NF040370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14045.1"
                     /translation="MSTPLRVAMWSGPRNISTAMMRAWENREDCSVSDEPLYAHYLAH
                     TGLDHPGRDEVIADGQTDWRRVVDALLGPAPGDAPVWYQKHMTHHLLPHIDHGWIGGL
                     RNVLLIRDPREVVASYVKSRAEIAPDDIGLRQQVELYDELSANGEAPPIIDSGDFLRA
                     PEAHLRALCGWLGIDFSERMLRWRKGPRASDGVWAPHWYAHVWQSTGFEAPEPRSIEL
                     SEAAQAVADACRPHYERLHALRLRVG"
     gene            complement(1869..>2603)
                     /locus_tag="PK399_12830"
     CDS             complement(1869..>2603)
                     /locus_tag="PK399_12830"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPW14046.1"
                     /translation="AKAIMLDIGMDRAALEAAIRDTLAANGMTTGSGVHIRLMVTRGV
                     KRTPYQDPRVNVGPATIVIIPEYKAASPEHAAHGITLFTVHVRRGFPDVQDPKLNSHS
                     KLNCITACIQAYTAGADEGLMLDPHGFVATCNSTHFFIVRKGEVWTSTGDYCLGGITR
                     ANVIAMCREAGIPVFQKNFSLTEAYGADEAFVTGTFAGVVPVRSIDGRAISAGRGPMV
                     ERLQGLYRDWLARDIAARKALAGATA"
BASE COUNT          389 a          845 c          917 g          452 t
ORIGIN      
        1 gcgcaccgtg ccctggtaga cgcggaagat gccgagcttg ccgacgaagg gatcgttgat
       61 gatcttgaac acgtcggcga tcacatgcgc ctgcgggtcg ggcgtggcct cgatcggctg
      121 cgcctcgtcg cccgagccct tcatgaaggg cggcggattg gcttcgcccg gatgcgggaa
      181 caggcgttcg gcgacatcca gcagttcgcg cacgccggcg ccgctgcgcg cggacacgaa
      241 gcacaccggc accaggtggc cctcgcgcag gcactgctcg aaggcgtcgt gcagctcctg
      301 cccggacaac ccgccttcgc cgagatcaag gtagtggtcc atgaccgtct cgttgacctc
      361 gaccacctgg tcgatgatcc ggcggtgcca gtcggcgact gggccgaggt tgctggtgcc
      421 ggtggattgc cagaaacagt cgacgaccga ctttccgccc tcgttgggca ggttgagcgg
      481 cagcacttcg ctgccgaagg tttcgcgcag gctgtcgagc aatgcgccga gtttcgcgcc
      541 cggatgatcg atcttgttga cgacgatcac ccggcagaga ccgcgcgcct tggcgtgcgc
      601 catcatccgc tgggtgccgt agctgatgcc ggtgtcggca tccaccacca ccgccaccgt
      661 ttccaccgcc gccagtgcgg acaaggtcgg gccgcggaaa tcgggatagc cgggggtgtc
      721 gatcaggttg acgtggatgg gcggttgccc gtgcggcgcg tggtcgatgc tggcgatcgc
      781 ggcgtcgatg gaatggctgc gctcgcgttc gattgggtcg aagtcggaca ccgtgctgcc
      841 gcgttcgata ctgcctgccg tctggatggc gccgccggca tgcagcaggg cttcgaacag
      901 ggtggtcttg ccggcgccgg ggtggcctgc caaggccacg ttgcggattt gctgtgtgct
      961 gtaggacatg agcccgttcc tccttcgcgt gcgaatggac gtggcgtcat ggctgtccgc
     1021 gctttgcgcc ggagcagtgc gctcggcggg cggtcatggc ggaatggaac attagcgcgg
     1081 aatccagcgg caatgtcgtg cgctgcaaca tgccggcggg gcgacgctgc ggtcgtgcta
     1141 gcccacgcgc agccgcagtg catgcagccg ttcgtaatgc ggcctgcagg catcggcgac
     1201 ggcctgcgcg gcctcgctca actcgatgct gcgcggctcc ggcgcctcga aaccggtcga
     1261 ttgccagaca tgcgcatacc agtgcggtgc ccagacgcca tcgctggcgc gaggcccctt
     1321 ccgccagcgc agcatgcgct cgctgaagtc gatgccaagc cagccgcaga gcgcgcgcag
     1381 gtgcgcttcc ggtgcacgca ggaaatcgcc ggaatcgatg atcgggggcg cttcgccatt
     1441 cgcggacaat tcgtcgtaca gctcgacttg ctgacgcagg ccgatgtcgt ccggcgcgat
     1501 ctccgcgcgc gacttcacgt aggaggcgac cacctcgcgc gggtcgcgga tcagcagcac
     1561 gttgcgcagc ccgccaatcc agccgtggtc gatgtgcggg agcaggtggt gggtcatgtg
     1621 cttctggtac cagaccggcg catcgccggg cgccgggccc agcagggcat cgacgaccct
     1681 gcgccagtcg gtctggccat cggcgatgac ctcgtcgcgg cccggatggt ccagcccggt
     1741 atgcgccagg tagtgcgcgt acagcggttc gtcgctgacc gagcagtcct cgcggttctc
     1801 ccacgcacgc atcatcgcgg tggagatgtt gcgcggcccg gaccacatcg ccacccgcag
     1861 cggcgtgctc atgcggttgc acccgccagc gccttgcgtg cggcgatgtc gcgcgccagc
     1921 cagtcgcggt acaggccttg cagccgctcc accatgggcc cgcgcccggc gctgatcgcg
     1981 cggccatcga tgctgcgcac cggcaccacg ccggcgaagg tgccggtgac gaaggcttcg
     2041 tcggcaccat aggcctcggt caggctgaaa ttcttctgga acaccgggat gccggcctcg
     2101 cggcacatcg cgatgacgtt ggcgcgggtg atcccgccca ggcagtagtc gccggtcgag
     2161 gtccagacct cgcccttgcg gacgatgaag aagtgggtgg agttgcaggt ggccacgaag
     2221 ccgtgcggat ccagcatcag gccctcgtcc gcgccggcgg tgtaggcctg gatgcaggcg
     2281 gtgatgcagt tgagcttgct gtgcgagttc agcttgggat cctgcacgtc ggggaaccca
     2341 cggcggacgt gcacggtgaa cagggtgatg ccatgtgccg cgtgttccgg cgaagcggcc
     2401 ttgtattcgg gaatgatcac gatggtggcc ggccccacgt taacgcgcgg gtcctggtag
     2461 ggcgtgcgct tgaccccacg ggtcaccatc aggcggatgt gcacgccgct gccggtggtc
     2521 attccattcg ctgccagcgt gtcacggatc gccgcttcca gcgcggcgcg atccatgccg
     2581 atgtcgagca tgatcgcctt ggc
//
LOCUS       DAOOCX010000229         2577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_40611_length_2577_cov_4.282316, whole genome shotgun sequence.
ACCESSION   DAOOCX010000229 DAOOCX010000000
VERSION     DAOOCX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40611_length_2577_cov_4.282316"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PK399_12835"
     CDS             <1..1019
                     /locus_tag="PK399_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW14047.1"
                     /translation="MLAYDGDAARGVDYAKRALAFAEKRRLAADSPTRVGLLRALSDT
                     QGIAGNLKEAEANVREAIRIESNINGADSPNLGKLYNSLGLVLGDQGRYREAIEAAER
                     AEALQAGTLAVPVETAIRTNNLAGAYENAGDYARAVALYADAMRVIERVDDGDAAMVR
                     RKVRVRQARAIGLAGDPARAVALLLSARQDIAAHEGPASMEYAFATLQLVSMARLQQD
                     PAHGEAWLAEARPLWAKLVGDDHLFFVLMARQAAAFARMRGDLAASESALLDAEKRLQ
                     GGRNAMIAAVTQAELASLHAARGDSADARRRLAAALPVLRTSVLPTQLDRVDAEALAR
                     SLGR"
     gene            complement(1035..2318)
                     /locus_tag="PK399_12840"
     CDS             complement(1035..2318)
                     /locus_tag="PK399_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14048.1"
                     /translation="MNMPTKTRIAVSLFVAIATLGACTRDATQSVEKPANGDYIVQDW
                     VLPAQPGSMAPDLRVAPDGRVLISWLNRQQGRRNVLQFASYTEAGGWQSLPRTIAVGQ
                     SLVANWADTPHLLATADGALWVQWLQANATSPAAYDVALARSRDGGMTWNQITRVNDD
                     TSGSEHGFAALWPRGASALGIAWLDGRDQHVPADAPQKQGAHHAGAMQLRANGFDMDL
                     ERGVDAVVDARTCDCCQTDVAITDRGPLLAWRDRDDDDVRDIVVARVQDDGTWTKPVA
                     VHADGWKVDACPVNGPAIAARGNDAVVAWYSEAGGTPAVRIARSTNAGDAFAAPIEVD
                     HGAVVRGQVDVAIDAQQAWVAWLREDARGQSLMLARYTPDLSRRLQLIEVAKLAARGH
                     ASGTPRLAVDAGGAWLAWTDAVDGAADLQGARIAR"
     gene            complement(2379..>2577)
                     /gene="gidA"
                     /locus_tag="PK399_12845"
     CDS             complement(2379..>2577)
                     /gene="gidA"
                     /locus_tag="PK399_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437137.1"
                     /note="GidA; glucose-inhibited cell division protein A;
                     involved in the 5-carboxymethylaminomethyl modification
                     (mnm(5)s(2)U) of the wobble uridine base in some tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPW14049.1"
                     /translation="RNETTAIPDAFDYAQVHGLSAEALQKLQRVRPQTIGQAQRIPGM
                     TPAAISLLLVHLERQRREQAA"
BASE COUNT          383 a          914 c          888 g          392 t
ORIGIN      
        1 cgatgctggc ctacgacggc gatgccgcgc gcggcgtcga ctacgcgaag cgtgcactcg
       61 ccttcgccga aaaacgcaga cttgccgcgg attcgccgac ccgggtcggc ctgttgcgtg
      121 cgttgtccga cacccagggc attgccggca acctgaagga ggcggaagca aatgtccgcg
      181 aagccatccg gatcgaatcc aacatcaacg gcgcggacag cccgaacctg ggcaagctct
      241 acaacagcct tggcctggtg ctgggcgatc agggccgcta tcgcgaggcg atcgaggcgg
      301 ccgagcgcgc ggaggcgctg caggccggca ccctggcggt gccggtggaa accgcgatcc
      361 gcaccaacaa cctggcaggt gcctacgaga atgccggcga ctacgcgcgc gcggtcgccc
      421 tgtatgccga tgcgatgcgc gtcatcgaac gtgtcgacga cggcgatgca gcgatggtca
      481 ggcgcaaggt gcgggtgcgc caggcgcgtg cgatcgggct ggctggcgat cctgcgcgcg
      541 cagtcgcgct gttgctttct gcgcgccagg acatcgccgc gcacgaaggc cccgcctcca
      601 tggagtacgc gttcgcgacc ctgcaactgg tgtcgatggc gcgcctgcag caggatcccg
      661 cgcatggcga ggcttggctg gccgaagcac gaccgctatg ggcgaagctg gtcggcgacg
      721 accatctgtt cttcgtgctc atggcgcgcc aggctgccgc gttcgcgcgc atgcgcggcg
      781 acctggcggc cagcgaaagc gcgctgctcg atgccgagaa acgccttcag ggtgggcgca
      841 acgcgatgat cgccgcggtc acccaggccg aactcgcctc gctgcatgca gcacgcggcg
      901 acagcgccga tgcccgtcgt cgcctcgccg cggcacttcc ggtgttgcgg acgtccgtgt
      961 tgccgaccca gctggatcgc gtcgatgcgg aggcgctggc gcgctcgctg gggcgctgac
     1021 cgggtcggcg ccactcaacg ggcgatgcgt gcgccctgca ggtcggccgc gccatccacc
     1081 gcatcggtcc aggccagcca cgcgccaccg gcatccaccg ccagccgtgg cgtaccgctg
     1141 gcgtggccgc gcgcagcgag cttcgccact tcgatcaact gcaaccgcct cgacaaatcc
     1201 ggggtgtagc gcgccagcat cagcgactgg cccctggcgt cctcgcgcag ccacgccacc
     1261 cacgcctgtt gcgcatcgat cgccacgtcg acctggcccc gcacgaccgc accatgatcg
     1321 acctcgatcg gtgccgcgaa ggcgtcgccg gcattcgtgc tgcgggcgat gcgcaccgcc
     1381 ggcgtgcccc cggcctcgct gtaccacgcc accacggcgt cattgccgcg cgccgcaatc
     1441 gccggaccgt tgaccgggca tgcatcgacc ttccagccat cggcatgcac cgccaccggt
     1501 ttcgtccacg tcccgtcgtc ctgcacgcgt gcgaccacga tgtcgcggac gtcatcgtca
     1561 tcgcgatcgc gccatgccag gagcgggcca cggtctgtga tcgcgacatc ggtctggcag
     1621 cagtcgcagg tgcgcgcatc gaccacggca tccacaccgc gctccaggtc catgtcgaag
     1681 ccgttggcgc gcagttgcat cgcgccggca tggtgcgcgc cttgcttctg cggggcatct
     1741 gccggcacgt gctggtcgcg cccatccagc caggcgatgc cgagcgcgct cgccccgcgc
     1801 ggccacaacg cggcgaagcc gtgttcgctg ccgctggtgt cgtcgttgac ccgggtgatc
     1861 tggttccagg tcatgccgcc atcgcgcgag cgcgccagcg ccacgtcgta ggcagccgga
     1921 ctggtggcgt tcgcctgcag ccactgcacc caaagcgcgc catcggcagt agccagcagg
     1981 tgcggcgtgt cggcccagtt ggcgaccagc gactggccca ccgcgatcgt gcgcggcagg
     2041 ctctgccagc cgccggcttc ggtgtacgag gcgaactgca gcacgttgcg ccggccctgc
     2101 tggcgattca gccagctgat caacacgcga ccatcggggg cgacgcgcag gtccggcgcc
     2161 atcgaaccgg gctgcgccgg cagcacccag tcctggacga tgtagtcgcc gttggcgggt
     2221 ttctcgacgg actgcgtggc atcgcgcgtg caggcgccga gcgtggcgat ggcgacgaag
     2281 agggaaactg cgatacgcgt cttggtcggc atgttcatgc gtgaaggata cgcccgccgg
     2341 ctgtggcgag cctcgggcct ggcgctaccg cgaatgcatc acgccgcctg ttcgcggcgc
     2401 tggcgttcca ggtggaccag cagcaatgaa atcgccgccg gggtcatgcc gggaatgcgc
     2461 tgcgcctggc cgatggtctg cgggcgcacg cgctgcagtt tctgcagggc ttccgccgac
     2521 aagccatgca cctgcgcgta gtcgaaggcg tcggggatcg cggtggtctc gttgcgc
//
LOCUS       DAOOCX010000230         2561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_40976_length_2561_cov_9.990423, whole genome shotgun sequence.
ACCESSION   DAOOCX010000230 DAOOCX010000000
VERSION     DAOOCX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40976_length_2561_cov_9.990423"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PK399_12850"
     CDS             <1..307
                     /locus_tag="PK399_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPW14050.1"
                     /translation="GMSSKSWDVFAAPDAPKMDFVITVCDSAAGEACPYWPGAPMTAH
                     WGFPDPSHVEGDDEARRHAFAKTAQAISQRLRLFLSLPIDKLDNLSLQDEVRALAAQ"
     gene            304..984
                     /locus_tag="PK399_12855"
     CDS             304..984
                     /locus_tag="PK399_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HPW14051.1"
                     /translation="MTPLHRRLLAEAVGTLLLLATVVGSGIMGVALSNGNDGIALLAN
                     AGATAGMLYVLIVVLGPISGAHFNPAVTLVMRLRGEMTSGDALAYIAVQVVAAVAGVV
                     LAHAMFDQALLQPGTHARSGGAQWVSEGVATCGLLLTILLGARYRPKAIPVLVASYIF
                     AAYWFTASTSFANPAVTLARSLTQSFAGIRPQDVGGFVLAQLAGALIAIGIARLLLAR
                     GETTSARG"
     gene            complement(970..2112)
                     /locus_tag="PK399_12860"
     CDS             complement(970..2112)
                     /locus_tag="PK399_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW14052.1"
                     /translation="MPATLPIDRDPILRAPRRRRLWWSLPLIGLIALTASLCLHASIG
                     DYANSAQFVDGKFRNTTPKPANVPEPSAKVMWDFFFNKPKNTEPHAPVPVHTLTRAEL
                     DAAPDRSLYRLGHSTILLKLRGHWWLTDPVFSERASPVQWAGPKRFHAPPIAIDELPP
                     IRGVLLSHDHYDHLDHAAIQALAGKVEVFLAPLGVGDRLAAWGVPNTRIRQFDWWQGA
                     DIAGLRLTFTPAQHFSGRGVRDGNRTLWGSWVIDDGQRRVFFSGDSGYFDGFAEIGRR
                     FGPFDLTLMETGAYNVQWPYVHMQPEETVRAHQDLRGGWLLPIHNGTFDLSMHPWFEP
                     FERVLALGDEHGIGIATPIMGERIDIDAPHAGERWWQSVVAESAAR"
     gene            2328..>2561
                     /locus_tag="PK399_12865"
     CDS             2328..>2561
                     /locus_tag="PK399_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HPW14053.1"
                     /translation="MTVIGIGLAADASAADLSGNATLTTDYVWRGSTQTQGDPAVQAG
                     FKVSGASGLYASAWGSNVKFAPETHASSELDFTI"
BASE COUNT          428 a          859 c          862 g          412 t
ORIGIN      
        1 cggcatgtcc agcaagagct gggatgtgtt cgccgcgccc gatgcgccga agatggattt
       61 cgtgatcacc gtctgcgaca gcgccgcagg cgaagcctgt ccgtactggc ccggtgcgcc
      121 gatgaccgcg cattgggggt tcccggatcc ttcgcatgtg gagggcgatg acgaagcccg
      181 ccgccatgcg ttcgcgaaaa ccgcacaggc catcagccag cgcctgcgcc tgttcctgtc
      241 gctgcccatc gacaagctcg acaacctgtc gctgcaggac gaagtgcgcg cgttggcggc
      301 acagtgacac cgctgcatcg ccgcctgctg gccgaagccg tcggcaccct gctgctgctc
      361 gccaccgtgg tcggctccgg gatcatgggc gtcgcattgt cgaacggcaa cgacgggatc
      421 gccctgctcg ccaatgcagg tgccaccgcc ggcatgctgt acgtgctgat cgtggtgctg
      481 ggtccgatct ccggcgcgca cttcaaccct gcagtgacgc tggtgatgcg gctgcgcggc
      541 gagatgacat cgggcgatgc gctcgcctac atcgcggtgc aggtcgtggc tgcggtcgcg
      601 ggcgtcgtgc tggcgcacgc gatgttcgat caagcgctgc tgcaacccgg cacccatgca
      661 cgcagcggcg gcgcgcaatg ggtcagcgaa ggggtggcga cctgcggcct tctgctcacg
      721 atcctgctcg gcgcgcggta tcggcccaag gcgatccccg tgctggtcgc cagctacatc
      781 ttcgccgcgt actggttcac cgccagcacc tcgttcgcca acccggcggt gaccctcgcg
      841 cgctcgttga cgcagagctt cgccggcatc cgtccgcagg acgtgggcgg cttcgtgctg
      901 gcgcaactgg ccggcgcctt gatcgccatc ggcatcgcac gactgttgct cgcgcgcggc
      961 gaaaccacct cagcgcgcgg ctgattccgc caccaccgac tgccaccagc gttcgcctgc
     1021 atgcggcgca tcgatgtcga tgcgttcgcc catgatcggg gtcgcgatgc cgatgccgtg
     1081 ttcgtcgccg agggcgagca cgcgctcgaa cggctcgaac cacggatgca tcgacaggtc
     1141 gaaggtgccg ttgtggatcg gcagcagcca accaccccgc aggtcctggt gcgcccgcac
     1201 ggtctcttcc ggctgcatgt gcacgtacgg ccattgcacg ttgtaggcgc cggtttccat
     1261 cagggtcagg tcgaacgggc cgaagcgccg gccaatctcc gcgaagccgt cgaagtagcc
     1321 gctgtcgcca ctgaagaaca cccggcgctg gccgtcgtcg atcacccaag aaccccacaa
     1381 cgtgcggttg ccgtcgcgca cgccgcgtcc ggagaaatgc tgcgcgggcg tgaacgtcaa
     1441 tcggaggccg gcgatgtcgg caccctgcca ccaatcgaac tggcggatcc tggtgttcgg
     1501 cacgccccac gcggccaggc ggtcgcccac accgagcggc gccaggaaca cctcgacctt
     1561 cccggccagc gcctggatcg cggcatggtc gaggtgatcg tagtggtcat gcgagagcag
     1621 cacgccgcgg atcggcggga gctcgtcgat ggcgatcggt ggcgcatgga aacgcttcgg
     1681 cccggcccac tgcaccggcg acgcgcgctc ggaaaacacc gggtcggtca gccaccagtg
     1741 cccacgcagc ttcaacagga tcgtcgaatg cccgagccgg tacaggctgc gatcgggcgc
     1801 ggcatcgagc tccgcgcgcg tgagtgtgtg caccggcacc ggcgcatgag gctcggtgtt
     1861 cttcggcttg ttgaagaaga aatcccacat caccttcgcg ctgggctccg gcacgttggc
     1921 tggcttcggc gtggtgttgc ggaacttgcc gtcgacgaat tgcgcggagt tggcgtagtc
     1981 gccgatcgaa gcgtgcaggc acagggaagc ggtcaaggcg atcagtccga tgaggggaag
     2041 cgaccaccac aagcgacgac gacgcggggc acgcaggatc ggatcgcggt cgatgggaag
     2101 ggtcgcaggc atgccgccaa cgacatgggg acgcagcagg cgcaggcaag tgcccgggat
     2161 ttacgccgaa tttacgcaac gccgcggccg gggcatggag ggtttatggc gccgacttcc
     2221 agaatttgcg ggtggcgatg acgccatgca acgtaccgcc ggctgcaatg aaaacaccct
     2281 ggttcaaggt gcgcgacgca cgcgcaccat cgatggcgac actcgccatg acggtcatcg
     2341 gcatcggcct tgccgccgat gcgagcgccg ccgacctcag cggcaacgcc actctcacca
     2401 ccgactatgt ctggcgcggc agcacgcaga cgcagggcga tcccgccgtg caggccggct
     2461 tcaaggtctc cggcgcatcc gggttgtatg cgtccgcatg gggctcgaac gtgaagttcg
     2521 cgccggaaac gcatgccagc agcgagctgg atttcaccat c
//
LOCUS       DAOOCX010000231         2528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_41826_length_2528_cov_9.417711, whole genome shotgun sequence.
ACCESSION   DAOOCX010000231 DAOOCX010000000
VERSION     DAOOCX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41826_length_2528_cov_9.417711"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..951)
                     /locus_tag="PK399_12870"
     CDS             complement(43..951)
                     /locus_tag="PK399_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14054.1"
                     /translation="MSIRPCPRLSLLAALLLGVAACAPPSLPFSRLAGLITTGQLDEI
                     SGLAASHVHEDVLWVINDGGNPAELYAINRLGRVLARYDVRGARNIDWEDLASFTLQG
                     KHYLLVADTGDNAGKRKDIVLHVFEEPARLDGGELKPAWTIRARWPDGPRDCEAVAVD
                     AAAGQVLLVSKKRNPPDLFALPLARSRGVREARRIGRLAGVPLASAELRRNDPRLARL
                     FPQVTAADLSPDGSTLAVLTYGSVLFYRRTPGENWSAAVARMPEAHDVPLIPQAEALA
                     WSAGGAGLYATGEFKPAPLFYLSPDR"
     gene            1044..2264
                     /gene="purT"
                     /locus_tag="PK399_12875"
     CDS             1044..2264
                     /gene="purT"
                     /locus_tag="PK399_12875"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HPW14055.1"
                     /translation="MPRSNPVTIGTPLSAHATRVLLLGSGELGKEVAIELQRFGVEVI
                     AADRYAHAPAMQVAHRSHVLDMLDGAAIRALIAQEKPGLVVPEIEAISTQTLVELEAE
                     GVARVIPTARAARLTMDREGIRRLAAETLGLATSPYRFVDTLEDYREAVSVLGLPCVV
                     KPVMSSSGKGQSLVRSEADIDAAWDYAQTGGRAGAGRVIVEGFIDFDYEITLLTVRHA
                     GGTDFCEPIGHLQRDGDYRESWQPQPMSALALQRSQEIARAITDDLGGWGLFGVELFV
                     KGDDVWFSEVSPRPHDTGLVTLVSQELSEFALHARAILGLPIHADASGAIRQLGASAS
                     CAVLAHGHGVPVFAGVDAALAHPDAQLRLFGKPRVEGHRRVAVTLARAADIDAARALA
                     RDAAAALTVTLQPA"
     gene            2278..>2528
                     /locus_tag="PK399_12880"
     CDS             2278..>2528
                     /locus_tag="PK399_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14056.1"
                     /translation="MNDSAGYAVFFFAPALEALGDAIKPYLQDGPAGPHVPCREVDTG
                     GAFIEMTLQGQSPDGRPIALELMVPGNMVRMIVSAHSDN"
BASE COUNT          393 a          875 c          868 g          392 t
ORIGIN      
        1 cacggcatgc agagaaggcg aagatctttg atgctcagcg tgtcagcgat ccgggctgag
       61 gtagaacaac ggcgccggct tgaactcgcc ggttgcgtac aggccggcgc cgcccgcact
      121 ccaggccagg gcctcggcct gcgggatcag cggcacgtcg tgcgcttcgg gcatgcgtgc
      181 caccgccgcg ctccagtttt cgcccggcgt gcgccggtag aacagcacgc tgccataggt
      241 cagcaccgcc agcgtgctgc cgtccggcga caggtcggcg gcggtgactt gcgggaacaa
      301 ccgcgccagt ctcggatcgt tgcggcgcaa ttccgcgctg gccagcggca ccccggccag
      361 gcggccgatg cggcgtgcct cgcggacgcc gcgcgaccgg gccaatggca gggcgaacag
      421 atccggcgga ttgcgcttct tcgacaccag cagtacctgg ccggccgcgg catcgaccgc
      481 caccgcctcg caatcgcgcg ggccatccgg ccaacgcgcg cggatcgtcc aggcgggctt
      541 caattcgccg ccgtcgaggc gcgccggttc ctcgaaaacg tgcagcacga tgtccttgcg
      601 tttgccggcg ttatcgccgg tatcggcgac cagcaggtaa tgcttgccct gcagggtgaa
      661 gctggcgagg tcttcccagt cgatgttgcg agcgccgcgc acgtcgtagc gggcaagcac
      721 ccggccgagg cggttgatcg catacagctc tgcgggattg ccgccatcgt tgatcaccca
      781 cagcacgtct tcatggacat gcgaggcggc caggccgctg atctcgtcca gttggccagt
      841 ggtgatgagg ccggccaagc gggagaacgg cagcgatggc ggcgcgcagg ccgccacgcc
      901 gagcagcagc gcggcgagca gcgacagccg cgggcagggg cggatcgaca tgcagcgagg
      961 atcgcatggc gatgcgcgcc ggcaacagcc cccgttgtcg cgcttcgcta gaattccggt
     1021 ttcccacgcc atggaacttg cgcatgcctc gatccaaccc ggtcacgatc ggcacgccgc
     1081 tgtccgcgca cgccacccgc gtgctcctgc tgggttcggg cgaactgggc aaggaagtgg
     1141 cgatcgagct ccagcgcttc ggggtggagg tgatcgccgc cgaccgctac gcgcacgcgc
     1201 ctgcgatgca ggtcgcccat cgcagccacg tgttggacat gctcgacggc gccgcgatcc
     1261 gcgcgctcat cgcccaggaa aagccgggcc tggtcgtgcc ggagatcgag gcgatcagca
     1321 ctcagaccct ggtggaactc gaagccgaag gcgtcgcccg ggtgatcccg acggcgcgcg
     1381 ccgcgcgcct gaccatggat cgcgaaggca tccgtcgcct cgccgcggaa acgctggggc
     1441 tggcgacatc gccgtaccgc ttcgtcgaca cgctggagga ttatcgcgaa gcggtgtccg
     1501 tgctcggact gccgtgcgtg gtcaagccgg tgatgtcctc ttccggcaag ggccagtcgc
     1561 tggtgcgttc cgaagccgac atcgatgctg cctgggacta cgcgcagacc ggcggccgcg
     1621 ccggcgccgg acgcgtgatc gtcgagggct tcatcgactt cgactacgag atcaccctgc
     1681 tcaccgtgcg ccatgccggc ggcaccgatt tctgcgagcc gatcggccac ctgcagcgcg
     1741 acggtgacta ccgcgagagc tggcagccgc agccgatgtc ggcgctcgcc ctgcaacgct
     1801 cgcaagagat cgcccgcgca atcaccgacg accttggtgg ctggggcctg ttcggcgtcg
     1861 agctgttcgt gaagggcgac gacgtctggt tcagcgaagt cagtccgcgc ccgcacgaca
     1921 ccggcctggt caccctggtc tcgcaggaac tcagcgaatt cgccctgcat gcgcgcgcga
     1981 tcctcggcct gccgatccat gccgacgcca gcggcgcgat ccgccagctc ggcgcatccg
     2041 cctcctgcgc cgtgcttgcg cacgggcatg gcgtgccggt gttcgcaggc gtcgatgccg
     2101 cgctcgcgca cccggatgcg caactgcggc tgttcggcaa accgcgtgtc gaggggcatc
     2161 gtcgcgtcgc ggtgacgctg gcacgcgctg cggacatcga cgcggcacgt gcgctcgccc
     2221 gcgatgccgc ggcggcgttg acggtcacct tgcagcctgc atgacagaat ccagcgcatg
     2281 aacgattccg ccggatatgc cgtgttcttc ttcgcccccg cgctggaggc gctgggtgat
     2341 gcgatcaagc cctacctgca ggatggcccc gccggcccgc acgtgccctg ccgcgaggtc
     2401 gacaccggcg gcgctttcat cgagatgacc ctgcaaggac aatcgccgga tggtcgtccg
     2461 atcgcgctgg agttgatggt gcccggcaac atggtgcgga tgatcgtgtc cgcgcacagc
     2521 gacaacga
//
LOCUS       DAOOCX010000232         2521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_42025_length_2521_cov_8.128143, whole genome shotgun sequence.
ACCESSION   DAOOCX010000232 DAOOCX010000000
VERSION     DAOOCX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42025_length_2521_cov_8.128143"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PK399_12885"
     CDS             complement(<1..952)
                     /locus_tag="PK399_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HPW14057.1"
                     /translation="MDLDHANDLQELAARACARLRAAGCEIDVGDEATIGKLAIASDF
                     AIATLVRQPALLARLQAGAAAPLAPPVLDAGNRAEWGRLLRLHRQAESTRMVWRDVIE
                     HAPVEEILAASTALADGCLQLALDALEAEMVQRFGVVRAADGGVQRLVVFGLGKLGGG
                     ELNFSSDVDLVYAYEHEGESDGARSLDAQDWFARLGQQLAKLLDEVTADGFSHRVDLR
                     LRPFGNAGRVALSFAAMEHYFLTEGRDWERYAWQKARPVAGDLVAGERLLQALRPFVY
                     RRYLDYGALDGLRTMKAAIAAEVARKDMADDIKRGPGGIRE"
     gene            995..2476
                     /locus_tag="PK399_12890"
     CDS             995..2476
                     /locus_tag="PK399_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW14058.1"
                     /translation="MSELQDLVALIRADTPLLVIETPDEARVVDLFRQSLMHAWRALY
                     RWSVTEGLRRIDMDREDEGIAPDLSATLQEIRNASQRGVYLLLDAHPYLGYASYQRAM
                     RDLIQRRDSQPHVLVMIGTKVELPAALEPFAVRFSLRLPDANALLKLVKEEAAAYTAQ
                     NGGRRVEVDADAVRQIVRNLQGLSLTDTRRIARHLIHRDGALGPGDLPELARLKFELL
                     NKSGHLHYEYDTARFADVGGARRLKRWVEQRRAPFVQDPPPPGLEPPKGVLLLGVQGC
                     GKSLLAKAVAGGFGVPLVRLDFGTLYDKYHGETEKNLRAALASTEQLAPCVLWIDEIE
                     KGLAAGNGESDGLSRRVLGYLLTWMAERKSRVFLVATANQVHDLPAELLRKGRFDEIF
                     FIDLPDAATRVEIFALHLDRRSLAKDDIDLSALAAAADGFSGAEIEQVIVSALYAAHA
                     EQAGLDTQTLMQAIRDTRPLSLLMAEQVQALREWALPRTVPAD"
BASE COUNT          414 a          873 c          857 g          377 t
ORIGIN      
        1 tctcgcggat gccgcccggg ccgcgcttga tgtcgtcggc catgtccttg cgcgcaacct
       61 cggcggcgat cgccgccttc atcgtgcgca ggccgtccag cgcgccgtag tcgaggtaac
      121 gccggtacac gaacgggcgc agcgcctgca gcaggcgctc gcccgcaacg aggtcacccg
      181 cgaccggccg cgccttctgc cacgcatagc gttcccagtc gcggccctcg gtgaggaagt
      241 agtgctccat ggcggcgaac gacagggcca cgcggccggc gttcccgaac ggacgcaggc
      301 gcaggtcgac gcgatggctg aagccatcgg cggtgacttc gtccagcagc ttcgccagtt
      361 gctggccgag ccgggcaaac cagtcctgcg cgtcgagcga acgcgcgcca tcggactcgc
      421 cctcgtgttc gtaggcgtag accaggtcga catcggaact gaaattcagc tcgccgccgc
      481 ccagcttgcc cagcccgaac accacgagcc gctgtacgcc gccatcggcg gcgcgcacca
      541 cgccgaagcg ctgcaccatc tcggcttcca gcgcgtccag cgccagttgc aggcagccat
      601 cggccagggc ggtgctggcg gccaggatct cctcgaccgg cgcgtgttcg atcacatcgc
      661 gccacaccat ccgggtcgat tccgcctgcc ggtgcaggcg cagcaggcgg ccccactcgg
      721 cgcgattgcc ggcatcgagc acgggaggcg cgagcggcgc ggcggcaccg gcctgcaatc
      781 tcgccagcag ggcaggctgg cgcaccagcg tcgcaatggc gaagtcgctg gcgatggcga
      841 gtttgccgat cgtggcttca tcgccgacat cgatttcgca acccgcagcg cgcaggcgcg
      901 cacaggcgcg cgccgcgagt tcctgcaagt cgttggcgtg atcaagatcc atgcccgatt
      961 gtggcatcgc atggccgccg ctatcgtgcc cgccatgagc gaattgcagg acctggtcgc
     1021 gctgatccgc gccgacacgc cgttgctggt gatcgaaacc cccgacgagg cgcgggtggt
     1081 cgacctgttc cggcaatcgt tgatgcacgc atggcgcgcg ctgtatcgct ggtcggtgac
     1141 agagggcctg cgtcggatcg acatggatcg cgaggacgaa ggcatcgccc ccgacctcag
     1201 cgcgaccctg caggaaatcc gcaatgccag ccagcgcggc gtgtaccttt tgctggacgc
     1261 acatccctac ctgggttacg ccagctacca gcgcgccatg cgcgacctga tccagcgccg
     1321 cgacagccag ccgcacgtgc tggtgatgat cggcaccaag gtcgagttgc cggcggcgct
     1381 ggagccgttc gcggtgcgct tctcgctgcg cctgccggat gcgaatgcac tgctcaagct
     1441 ggtcaaggaa gaagccgcgg cctacaccgc ccagaacggc gggcgccggg tcgaagtgga
     1501 tgccgatgcg gtgcgccaga tcgtgcgcaa cctgcagggc ctgagcctga ccgacacgcg
     1561 ccggatcgcg cgacacctga tccatcgcga tggcgcgctg gggcccggcg acctgccgga
     1621 actggcacgg ctgaagttcg agctgctcaa caagagcggc cacctgcatt acgaatacga
     1681 caccgcgcgc ttcgccgacg taggcggtgc gcgcaggctg aaacgctggg tggaacagcg
     1741 gcgtgcgccc ttcgtgcagg atccgccgcc gcccgggctg gaaccgccga agggggtgct
     1801 gttgctgggc gtgcagggct gcggcaagtc gttgctggcg aaggcggtcg ccggcggctt
     1861 cggggtgccg ctggtgcggc tcgacttcgg caccctgtac gacaagtacc acggcgagac
     1921 cgagaagaac ctgcgcgccg cgctggcctc caccgaacag ctcgcgccct gcgtgctgtg
     1981 gatcgacgag atcgagaagg gcctggccgc cggcaatggc gagagtgatg gcctgtcccg
     2041 ccgcgtgctg ggctacctgc tgacgtggat ggccgaacgc aagtcgcgcg tcttcctggt
     2101 cgccaccgcc aaccaggtgc atgacctgcc ggcggaactg ctgcgcaagg gccgcttcga
     2161 cgaaatcttc ttcatcgacc tgcccgacgc tgctacccgc gtcgagatct tcgccctgca
     2221 cctggaccgg cgttcgctgg ccaaggacga catcgacctg tcggcgcttg ccgccgcagc
     2281 cgacggcttc tccggcgcgg agatcgagca ggtgatcgtc agtgcgctgt atgccgcgca
     2341 tgcggaacag gccgggctcg acacacaaac cctgatgcag gcgatccgcg acacccgccc
     2401 gctgtccttg ctgatggcgg aacaggtgca ggcgctgcgc gaatgggcct tgccgcgaac
     2461 ggtgccggcg gactgaaagg acgccgagcg cgcgcctgca aagccctcgt gcataaaaaa
     2521 g
//
LOCUS       DAOOCX010000233         2500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_42556_length_2500_cov_5.606544, whole genome shotgun sequence.
ACCESSION   DAOOCX010000233 DAOOCX010000000
VERSION     DAOOCX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42556_length_2500_cov_5.606544"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK399_12895"
     CDS             <1..188
                     /locus_tag="PK399_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14059.1"
                     /translation="MVPGNMVRMIVSAHSDNEFGFYDRNRPQAMLEPGMPVIAPPDAT
                     PAAEAPPVQSPVPATAA"
     gene            complement(232..1455)
                     /locus_tag="PK399_12900"
     CDS             complement(232..1455)
                     /locus_tag="PK399_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPW14060.1"
                     /translation="MKPCIASWLVACAAMLLAACAATTPHTREQDAMASTPTGTLRVA
                     TYNTSLFDEDAGGLVARLDFGNAQARKIAAVLQRVRADIVLLNEFDYDASGQAADLFQ
                     RRYLEVAQAGGGEPLRYEHRYLAPVNTGVPSGLDLDGNGETDGVGRDRGNDAWGYGLH
                     PGQYGMLVLSRFPIDEAAVRTFQLLRWDAMPGALRPMVPDTGKPFHADAVWTQLRLSS
                     KSHWDVPIQTPSGTLHLLASHPTPPTFDGPEDRNGARNADEIRLWREYIARQDASWLC
                     DDAGRCGGLAGDARFVIAGDLNNDPVDGAGHHDAILELLEHPRVLRMATPRSAGGEAK
                     AREYAAAGIARRGAPAHVTGDFGPNVGALRLDYVLPSVGFALVGSGVHWPAPGDPDAV
                     FADGSDHHLVWVDLR"
     gene            complement(1452..2171)
                     /locus_tag="PK399_12905"
     CDS             complement(1452..2171)
                     /locus_tag="PK399_12905"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HPW14061.1"
                     /translation="MGTETDNLRMFHTGAHPCGYWEGREARDLVLDPRDPRLPQAYPM
                     ALDWGFRRSGDIVYRPSCSGCHACVAVRVPVDGFRPDRSQRRCLARNADIGARIVAPV
                     RTDEHLALYRKYLASRHAGGGMDDHGANEFDQFLVGSWNEGRFLELREHGTHKLLAVA
                     VTDLVEHALSAVYTFYDPDHASRGLGTLGVLQQIAWARRDGREHLYLGYWIAGHAKMD
                     YKRRFQPLEGFNGRGWGRLQA"
     gene            2281..>2500
                     /locus_tag="PK399_12910"
     CDS             2281..>2500
                     /locus_tag="PK399_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14062.1"
                     /translation="MKKLHILALAITAVLASSFAVAQTATPATGVKAAPRIDANGDGV
                     IDRAEAAKMPRLAEKFDQLDIDKDGKLSA"
BASE COUNT          394 a          855 c          876 g          375 t
ORIGIN      
        1 tgatggtgcc cggcaacatg gtgcggatga tcgtgtccgc gcacagcgac aacgaattcg
       61 gcttctacga ccgcaatcgc ccgcaggcga tgctggaacc gggcatgccg gtcatcgcgc
      121 cccccgatgc aactcctgcc gccgaagcgc cgccagtgca gtcgccggtc cccgcaaccg
      181 cggcttgacc gcgcgcattc gcgtggccgt ggtgtaacgc ggctcgttcg ctcagcgcag
      241 gtcgacccag accaggtggt ggtcgctgcc gtcggcgaag accgcgtcgg gatcgccggg
      301 cgctggccag tgcacgccgc tgccgaccag ggcgaagccc accgatggca acacgtagtc
      361 cagccggagc gcgccgacgt tcgggccgaa gtcgccggtg acgtgcgccg gtgcgccacg
      421 tcgtgcgatg ccggccgccg cgtactcacg cgccttggcc tcgccgcccg cactgcgcgg
      481 ggtggccatg cgcagcaccc gtggatgctc gagcagttcg aggatcgcat cgtggtggcc
      541 ggcgccatcg accggatcgt tgttgaggtc gccggcgatc acgaaacgcg catccccggc
      601 gagtccgccg caacggcccg cgtcatcgca cagccacgat gcgtcttgcc gcgcgatgta
      661 ttcgcgccac aggcgtatct cgtctgcgtt ccgcgcgcca ttgcgatcct cggggccgtc
      721 gaaggtcggc ggggtcgggt gcgaggccag caaatgcagc gtgcccgacg gcgtctggat
      781 cggtacgtcc cagtgcgatt tcgacgacag ccgcagttgc gtccagacgg catcggcatg
      841 gaagggcttg ccggtgtccg gcaccatcgg ccgcagcgca ccgggcatcg cgtcccagcg
      901 cagcaactgg aacgtgcgca cggccgcttc gtcgatcggg aagcgcgaca gcaccagcat
      961 gccgtactgg ccgggatgca ggccgtagcc ccatgcatcg ttgccgcgat cgcgaccgac
     1021 gccatcggtc tcgccattcc cgtccaggtc gaggccgctg ggcacgccgg tgttcaccgg
     1081 tgccaggtag cgatgctcgt agcgcagcgg ctcgccgcca ccggcctgcg cgacctcgag
     1141 gtaacgccgt tggaacaggt cggcagcctg gccggaagcg tcgtaatcga attcgttgag
     1201 caggacgatg tccgcacgga cccgctgcag cacggcagcg atcttgcgcg cctgcgcgtt
     1261 gccgaagtcc agccgcgcga ccaggccgcc ggcatcctcg tcgaacaacg aggtgttgta
     1321 cgtcgccacg cgcagcgtgc cggtcggcgt cgatgccatg gcgtcctgtt ccctggtgtg
     1381 aggtgtggtg gccgcgcagg cggccagcag cattgcagcg caggccacca gccacgatgc
     1441 aatgcagggc ttcatgcctg caggcgtccc cagccgcggc cgttgaatcc ttccagcggc
     1501 tggaaacggc gcttgtagtc catcttcgcg tgtcctgcga tccagtagcc gagatagagg
     1561 tgctcgcggc cgtcgcggcg cgcccaggcg atctgctgca gcaccccgag cgtaccgagc
     1621 ccgcgcgagg catggtcggg atcgtagaag gtatagaccg cggacagcgc atgctcgacg
     1681 aggtcggtga ccgcgaccgc gagcagtttg tgggtgccgt gctcgcgcag ttcgaggaag
     1741 cggccctcgt tccacgagcc caccaggaac tggtcgaatt cgttggcgcc gtggtcgtcc
     1801 atgccgccac cggcatgccg gctcgcgagg tacttccggt agagggcgag gtgttcgtcg
     1861 gtgcgcaccg gcgcaacgat gcgcgcaccg atgtcggcat tgcgcgccag gcatcggcgt
     1921 tggctgcggt ccgggcggaa cccgtcgacc gggacgcgca ccgcgacgca ggcatggcag
     1981 ccgctgcagc tcggccggta gacgatgtcg cccgaacgac ggaaacccca gtccagcgcc
     2041 atcggatagg cctgcggcag gcgcgggtcg cgcggatcca ggaccaggtc gcgggcctcg
     2101 cggccctccc aatacccgca gggatgggcg ccggtgtgga acatgcgcag gttgtcggtc
     2161 tcggtaccca tgcggacagg atagcgccgc cgttcagcgg ggcgcaggcc gtgctgtcaa
     2221 ccgttggtgg cgcacggcgt ttcccatgca agcccggcga gtgccggtag gagacgaacc
     2281 atgaagaaat tgcacatcct ggccctggcg atcaccgccg tgctggcgtc cagcttcgcg
     2341 gtcgcgcaga ccgccacccc ggcgaccggc gtgaaggccg cgccgcgcat cgacgccaat
     2401 ggcgatggcg tgatcgatcg cgccgaggcc gccaagatgc cgcggctggc ggagaagttc
     2461 gaccagttgg acatcgacaa ggacggcaag ctcagcgcca
//
LOCUS       DAOOCX010000234         2465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_43474_length_2465_cov_7.641494, whole genome shotgun sequence.
ACCESSION   DAOOCX010000234 DAOOCX010000000
VERSION     DAOOCX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43474_length_2465_cov_7.641494"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2465
                     /locus_tag="PK399_12915"
     CDS             <1..>2465
                     /locus_tag="PK399_12915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPW14063.1"
                     /translation="VTPHADRMTFDGKVRIDIEVLAPTSSIVLQAANLTFGTATLAAK
                     GGKPQAAKVSVDAELQTATFAFDNALAPGRYTLSADYAGVINTQANGFFALDYQTESG
                     KKRALYTQFENSDARRFVPSWDEPNFKATFDLTVNAPSADMVVSNMPEASSKDLGNGM
                     KQVVFQTSPKMSTYLLFLGLGEFERATLQGENGKGKPVEIGVIAQKGKVEQARFALEG
                     SRDVLREYNDYFGVPFPLPKLDNIAAPGRSQFFSAMENWGAIFTFESTLLLDPAISNV
                     SDQQRVFTVAAHEIAHQWFGNLVTMAWWDDLWLNEGFATWMEGRTTRKLHPEWDTDHV
                     EAAFTSRGSMGRDAYATTHPVVQHIATVEQASQAFDGITYGKGSAVIAMLEDYVGADA
                     WRDGVRSYIAKRAYGNAVTDDLWHEIDKAAPGKQFIDVAHDFTLQPGVPLVKASATCD
                     GGRTVVSLEQGEYTVDRPGKTPLRWRVPVTLRSGDSAPVRVLVDGKASVALPGCGAPL
                     LVNAGQNGYYRTLYAPAQFKALTDRFASLPVVDQAGVMMDAGALAAVGLQPESDSLDL
                     TTKVALDASPALWQMLAGTVGGIDDLFEGDAKRQAAARAYAVSRLSPKFQQLGWDNRE
                     GDSATTRQLRSSLIGILSGLGDQEVIAEARRRFTASLTDPKALSPDLRRTVLGIVARN
                     ADAATWDQLHALAQAEKSSMIRDQYYGLLAAPKDKALAQRALDMALTDEPGATNSAGI
                     ISGVSREHPELAFDFAVAHREQVDKLVDSTSRARFYPGLASGAADVAMVAKIKAFAEA
                     HIAATSRRDAETVMSGIETRIKLR"
BASE COUNT          423 a          849 c          829 g          364 t
ORIGIN      
        1 gtcaccccgc atgcggacag gatgacgttc gacggcaagg tccggatcga catcgaggtg
       61 ctggcgccca cgtccagcat cgtcctccag gccgcgaacc tcacgttcgg caccgccacg
      121 ctggcggcca agggcggcaa gccgcaggcc gccaaggtca gcgtggatgc cgagttgcag
      181 accgcgacct tcgccttcga caacgcgctg gcaccgggcc gctacacgct gtcggccgac
      241 tatgccggcg tgatcaatac ccaggccaac ggcttcttcg cgctcgacta ccagaccgag
      301 tcgggcaaga agcgcgcgct gtacacccag ttcgagaact ccgatgcgcg ccgcttcgtg
      361 ccttcgtggg acgagcccaa cttcaaggca acgttcgacc tgacggtcaa tgcgccgagc
      421 gccgacatgg tggtcagcaa catgccggag gcgtcctcga aggacctcgg caacggcatg
      481 aagcaggtgg tgttccagac ctcgccgaag atgtcgacct acctgctgtt cctgggcctg
      541 ggcgagttcg agcgcgccac cctgcagggc gagaacggca agggcaagcc ggtcgagatc
      601 ggcgtgatcg cgcagaaggg caaggtcgag caggcgcgct tcgcgctgga aggcagccgc
      661 gacgtgttgc gcgaatacaa cgactacttc ggcgtgccgt tcccgctgcc gaagctcgac
      721 aacatcgccg cgcccgggcg cagccagttc ttcagcgcga tggagaactg gggcgcgatc
      781 ttcaccttcg aatccacctt gttgctggat ccggccatct ccaacgtctc cgaccagcag
      841 cgcgtgttca ccgtggccgc gcacgagatc gcccaccagt ggttcggcaa cctggtcacc
      901 atggcgtggt gggacgacct ctggttgaac gaaggcttcg ccacatggat ggaaggccgc
      961 accacccgca agctgcatcc ggaatgggat accgaccacg tcgaggccgc cttcaccagc
     1021 cgcggttcga tgggccgcga tgcctatgcg accacccacc cggtggtgca gcacatcgcc
     1081 accgtcgaac aggccagcca ggccttcgac ggcatcacct acggcaaggg ctcggcggtg
     1141 atcgccatgc tggaggacta cgtgggcgcc gatgcctggc gcgacggcgt gcgcagctac
     1201 atcgccaagc gcgcgtacgg caacgcggtc accgatgacc tctggcacga gatcgacaag
     1261 gccgcgccgg gcaagcagtt catcgacgtg gcccacgatt tcaccctgca gcccggcgtg
     1321 ccgctggtga aggccagcgc cacctgcgat ggcggcagga ccgtggtctc gctggagcag
     1381 ggcgaataca ccgtcgaccg tcccggcaag acgccgttgc gctggcgcgt cccggtcacc
     1441 ctgcgcagtg gcgatagcgc gccggtgcgc gtgctggtgg atggcaaggc cagcgtcgca
     1501 ctgcccggtt gcggcgcgcc gttgctggtc aacgccggcc agaacggcta ctaccgcacc
     1561 ctgtacgcgc cggcgcagtt caaggcgctt accgatcgct tcgcgagcct gccggtggtc
     1621 gaccaggccg gcgtgatgat ggatgcaggc gcgttggccg ccgtgggcct gcagccggaa
     1681 tccgattcgc tggacctcac caccaaggtg gcgctggatg cgtccccggc gctctggcag
     1741 atgctggccg gcacggtggg cggcatcgac gacctgtttg aaggcgacgc caagcgccag
     1801 gcggcggccc gcgcctatgc ggtgtcgcgg ctgtcgccga agttccagca actcggctgg
     1861 gacaaccgcg agggcgattc cgcgacgacc cggcaactgc gctcgagcct gatcggcatc
     1921 ctcagcgggc tgggcgacca ggaggtgatc gcggaagcgc gtcgccgctt caccgcgtcg
     1981 ctcaccgatc ccaaggcgct gtcgcccgac ctgcgccgca cggtgctggg catcgtcgcc
     2041 cgcaacgcgg acgcggccac ctgggaccag ctgcatgcgc tggcgcaggc cgagaagtcc
     2101 tcgatgatcc gcgaccagta ctacggcctg ctggcggccc cgaaggacaa ggcgctggcg
     2161 cagcgcgcgc tggacatggc gttgaccgac gagccgggcg cgaccaacag cgccgggatc
     2221 atttccggag tctcccgcga gcatccggag ctggccttcg acttcgccgt ggcccatcgc
     2281 gagcaggtcg acaagctcgt cgattcgacc tcgcgcgcac gtttctaccc cggcctggcc
     2341 agcggcgcgg ctgacgtggc gatggtcgcg aagatcaagg cgttcgccga agcgcacatc
     2401 gccgcgacct cgcgacgcga tgcggagacg gtgatgtccg gcatcgagac ccggatcaag
     2461 ctgcg
//
LOCUS       DAOOCX010000235         2440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_44180_length_2440_cov_4.826834, whole genome shotgun sequence.
ACCESSION   DAOOCX010000235 DAOOCX010000000
VERSION     DAOOCX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44180_length_2440_cov_4.826834"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PK399_12920"
     CDS             complement(<1..379)
                     /locus_tag="PK399_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HPW14064.1"
                     /translation="MAAPTTSHRKALARRRQQLMQAAGDGSILILPAAPERIRSRDTH
                     YPYRQDSDFWYLTGCAEPDAVLVLVPGRKHGEAILFCRERDPEREGWDGPRLGPDGAV
                     ELLGMDDAYPISDIDDILPGLLEG"
     gene            complement(396..938)
                     /locus_tag="PK399_12925"
     CDS             complement(396..938)
                     /locus_tag="PK399_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HPW14065.1"
                     /translation="MSETELPAWADVATAADRLELASTPAELHGALCGWLAAGGADQA
                     SWIATVMADPGLPAPTPGDALDLLRAASAQQLGDADFGFELLLPDDEDVQVRAGGLFA
                     WCRAFLGGFGLAVGDKPLSDDGKEALGDLANLAAARIEEVDPEQDEESLTEIEEYLRM
                     AVLLLHADCALGPRSRQRLH"
     gene            1030..>2440
                     /locus_tag="PK399_12930"
     CDS             1030..>2440
                     /locus_tag="PK399_12930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW14066.1"
                     /translation="MGIRAITPSSLAGAAAPVPASSSLLDMPSSHVLALLVALLVLAM
                     AGMAIAGHRRRDREQRDHLRAMREREQRLRLSLWASNEIYWQYDLEKRELERTRVEPD
                     RSDDLAVQVDLDKDHQIHEEDLPLVLERLREYVTGGTQMFLSEHRILGEGGQWQWMRA
                     RGRAIARDATGRVTRIAGTARNVTSIREKERERRIANEVMHNMAESVAVVDAEFRFVA
                     VNPAFTRMSGYEASDVIGKDGSIINSDQQDEAFYEKSRKAMQQQGCWSGEMWQKRKDG
                     RDFLCAVQFNAIVEPGTRKRLYVVVASDITDRRRIEQELRYLANYDTLTNLPNRTLLA
                     ERLSRAIVRARRQGTRLALLFLDLDRFKHINDSLGHATGDRILRAAAQRLQDVVGDNH
                     TVARIGGDEFTVLLEDLSGAAEAERCAQHIIAAFDAPLRLDDRHEIAISPSIGISMFP
                     DHAQVPTDLLKHADTAMYQA"
BASE COUNT          395 a          890 c          807 g          348 t
ORIGIN      
        1 ggccttccag caggccgggg aggatgtcgt cgatgtccga gatcggatac gcatcatcca
       61 tgcccagcag ctcgaccgca ccatccggcc ccagccgcgg accgtcccag ccctcgcgtt
      121 ccggatcgcg ttcgcgacag aacaggatcg cctcgccgtg cttgcgcccg ggcaccagca
      181 ccagcacggc gtcgggttcg gcacagccgg tcaggtacca gaagtcggaa tcctggcggt
      241 acgggtaatg ggtgtcgcgg ctgcggatgc gctccggtgc ggcgggcagg atcaggatgg
      301 aaccgtcgcc agccgcctgc atcagctgct gccggcgacg cgccagcgcc ttgcggtggc
      361 tcgtggtggg cgcggccatg cggacgcccg ccgcctcagt gcaggcgctg gcggctgcgc
      421 gggccaagcg cgcagtccgc gtgcagcagc agtaccgcca tgcgcaggta ttcctcgatc
      481 tcggtcaggg attcctcgtc ctgctccgga tccacttcct cgatccgcgc ggcggccagg
      541 ttggccaggt cgcccagggc ttccttcccg tcgtcggaca ggggcttgtc gccgaccgcc
      601 aatccgaacc cgcccaggaa cgcacggcac cacgcgaaca acccgccggc acgcacctgc
      661 acgtcctcat cgtcgggcag cagcagctcg aagccgaaat ccgcatcgcc cagctgctgc
      721 gcgctggcgg cgcgcagcag gtccagtgca tcgcctggcg tgggtgccgg aaggccgggg
      781 tcggccatca ccgtggcgat ccagctggcc tggtccgccc cgcccgccgc cagccacccg
      841 catagcgcgc catgcagttc ggcgggggtg ctggccagtt ccagccggtc ggcggcggtg
      901 gccacgtcgg cccaggccgg cagctcggtt tcgctcacgg ggatgcgcct tgatgcaggg
      961 ccgggcagtt tagcaatcca tgcccgggcc ggcttgttgc cggcgccggc ccgcgcctac
     1021 actggccgga tggggatccg cgccatcacg ccatcaagcc tggccggcgc cgccgcgcca
     1081 gtgcctgcgt cctcttcgct gctggacatg ccgtccagcc acgtgctcgc gctgctggtc
     1141 gcgttgttgg tgctggcgat ggccggcatg gcgatcgccg ggcatcgccg ccgcgatcgc
     1201 gaacaacgcg accacctgcg cgcgatgcgc gagcgcgagc agcgcctgcg gctgtcgctg
     1261 tgggcctcca acgagatcta ctggcagtac gacctggaaa agcgcgagct ggagcgcacc
     1321 cgggtcgagc ccgaccgcag cgacgatctc gccgtgcagg tcgacctgga caaggaccac
     1381 cagatccacg aggaagacct gccgctggtg ctggaacggc tgcgcgagta cgtcaccggc
     1441 ggcacccaga tgttcctctc cgaacaccgg atcctgggcg aaggcgggca atggcagtgg
     1501 atgcgcgcgc gcggccgcgc gatcgcccgc gacgccaccg gccgggtcac ccgcatcgcc
     1561 ggcaccgcgc gcaacgtcac ctcgatccgc gagaaggaac gcgagcgccg catcgccaac
     1621 gaggtcatgc acaacatggc cgaatcggtg gcggtggtgg atgccgagtt ccgcttcgtc
     1681 gcggtcaacc ccgccttcac ccgcatgagc ggctacgagg ccagcgacgt gatcggcaag
     1741 gatggctcga tcatcaacag cgaccaacag gacgaggcct tctacgaaaa gtcgcgcaag
     1801 gccatgcaac agcagggctg ctggagcggc gagatgtggc agaagcgcaa ggacgggcgc
     1861 gacttcctgt gcgcggtgca gttcaacgcg atcgtcgaac cgggcacccg caagcgcctg
     1921 tacgtggtcg tggccagcga catcaccgac cgtcgccgga tcgagcagga gctgcgctac
     1981 ctcgccaact acgacaccct gaccaacctg cccaaccgca ccctgctggc cgaacgcctg
     2041 tcccgtgcga tcgtccgcgc gcgccgtcag ggaacccgtc tggcgctgct cttcctcgac
     2101 ctcgaccgct tcaagcacat caacgattcg ctggggcatg ccaccggcga tcgcatcctg
     2161 cgcgccgctg cgcaacgcct gcaggacgtg gtcggcgaca accacaccgt cgcccgcatc
     2221 ggcggcgacg aattcaccgt gctgctcgag gatctctccg gtgccgcaga ggccgagcgc
     2281 tgcgcgcaac acatcatcgc ggccttcgac gccccgctgc ggctcgacga ccgccacgag
     2341 atcgcgattt cgccgtcgat cggcatcagc atgttcccgg atcatgcgca ggtgcccacc
     2401 gacctgctca agcacgccga caccgcgatg taccaggcca
//
LOCUS       DAOOCX010000236         2404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_45206_length_2404_cov_4.156662, whole genome shotgun sequence.
ACCESSION   DAOOCX010000236 DAOOCX010000000
VERSION     DAOOCX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45206_length_2404_cov_4.156662"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PK399_12935"
     CDS             complement(<1..505)
                     /locus_tag="PK399_12935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017113267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW14067.1"
                     /translation="MSGEGIAAIVVSHDSEETLDACLSGLRAAEGVVEIRVVDNNSGD
                     ASMEVAQRHALADPRLRFIANPDNPGFAVACNQGAAASAAPWLAFVNPDCFVEVDTLS
                     RMRAHAWRAMGFDGAGPSSIEAYFDVAMRGKSGDIVLGADLVDEDGMRDAAARRRDPD
                     FAAMLRNP"
     gene            complement(502..1365)
                     /locus_tag="PK399_12940"
     CDS             complement(502..1365)
                     /locus_tag="PK399_12940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006453027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW14068.1"
                     /translation="MAGAPLPIASLPVVIVPVGTDEDALDACLAALDATTPAGTRVWL
                     ADDAQAGPRGNAIIERWLAQTPLQAAHTRRASPIGEAAHLDEALRACGNGDVAILASD
                     ARPAPGWLERLQECLAHDPAIATATPWSNAGEAASWPRIGEIVQMDASPARLARACAQ
                     LGNAHPPLPSAVTHAVLLRGAARMRAGGLDPASYRSWYAALIDLSLRLSGLGWRNALC
                     ETAFVARNVEGQPADGDMDALSVRWPAWHAQLAHFLMGDPLRSLRERMGDALARLDRA
                     PPQPDLFADAP"
     gene            complement(1368..>2404)
                     /locus_tag="PK399_12945"
     CDS             complement(1368..>2404)
                     /locus_tag="PK399_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPW14069.1"
                     /translation="KSGTSSAAVNTGLGDGGNLPANRSGNGKVTLKVEGAEVGVSAVE
                     DTNSLLVRASPSQWKSIREVIERIDVMPMQVHIEAQVISVALKGALQYGVSWFFDNAV
                     GEAGFPYPTGRNSFGSYSGGISQPNVPNGGSIGNLLSWTFLGNNAAAIVSALDNVTDV
                     RTLSAPSVFTQNNREASLNVGQRIPISSVSLNPGNGNDNTYSSVQYLDTGIILKVRPR
                     VTRDGLVFLDIVQEVSKPTGEADDNGNVRIDTSKVTTSALAPSGETIVLAGLISDGTS
                     RNATGVPGLSRIPILGGLFGQQGQTKTRDELVILITPTVVRNPMEARNLTDEYGRRFR
                     ALDPIYKPKQ"
BASE COUNT          361 a          811 c          834 g          398 t
ORIGIN      
        1 atggattgcg cagcatcgcc gcgaagtccg gatcgcgacg acgcgccgcg gcatcgcgca
       61 tgccatcctc gtcgaccagg tcggcaccga gcacgatgtc gccacttttc ccgcgcatgg
      121 cgacatcgaa gtacgcctcg atactggacg ggccggcgcc gtcgaaaccc atcgcgcgcc
      181 aggcatgcgc gcgcatgcgc gacagcgtgt cgacctcgac gaagcaatcc gggttcacga
      241 acgccagcca aggcgcagcg ctcgccgctg cgccctggtt gcaggccacc gcgaagccgg
      301 ggttgtccgg attggcgatg aagcgcaggc gcggatcggc cagcgcgtgc cgctgcgcga
      361 cttccatgct ggcatcgccc gagttgttgt cgaccacgcg gatttcgacc acgccttcgg
      421 cggcgcgcag gcccgacagg caggcgtcga gggtttcctc gctgtcgtgg ctgaccacga
      481 tcgcggcgat accctcgccg ctcatggcgc gtccgcgaac aggtccggtt gcggcggcgc
      541 cctgtccagg cgcgcgagtg cgtcacccat gcgttcgcgc agcgagcgca gcggatcgcc
      601 catcaggaaa tgcgccagct gcgcatgcca ggccggccag cgcaccgaca acgcgtccat
      661 gtcgccatcc gccggttgtc cttcgacatt gcgggcaacg aatgcggttt cgcacaacgc
      721 gttgcgccag cccagtcccg acaggcgtag cgagagatcg atcaacgccg cgtaccacga
      781 ccgataactg gccgggtcga ggccaccggc acgcatgcgt gccgcaccac gcagcagcac
      841 cgcatgggtc accgccgacg gcaagggcgg atgtgcgttg ccgagttgcg cgcaggcacg
      901 cgccagccgt gccggcgacg catccatctg gacgatctcg ccgatgcgcg gccatgaggc
      961 ggcttcgccg gcgttgctcc agggcgttgc ggtggcgatg gcgggatcgt gcgccaggca
     1021 ctcctgcaag cgctccagcc agcccggcgc ggggcgcgca tcgctggcca ggatcgccac
     1081 gtcgccattg ccgcaggcgc gcagggcttc gtcgaggtgc gcggcttcgc cgatcggcga
     1141 tgcgcggcgc gtatgcgcgg cctgcaatgg cgtctgcgcc agccagcgtt cgatgatcgc
     1201 gttgccgcgc gggccggcct gcgcgtcgtc ggccagccag acccgcgttc ccgcgggcgt
     1261 cgtggcatca agcgcggcca ggcaggcgtc gagcgcgtcc tcgtcggtgc cgaccggcac
     1321 gatcaccacc ggaagggatg cgatgggaag gggcgcgccc gccaggctca ctgcttcggc
     1381 ttgtagatcg gatccagcgc gcggaaacgg cgaccgtatt cgtcggtgag gttgcgcgct
     1441 tccatcgggt tgcgcaccac ggtcggggtg atcaggatca ccagttcgtc gcgggtcttg
     1501 gtctggccct gctggccgaa caggccaccg aggatcggga tccggctcag cccgggcacg
     1561 ccggttgcat tgcggctcgt gccatcgcta atcaggccgg ccagcacgat ggtctcgccg
     1621 ctcggcgcca gcgcactggt ggtgaccttg ctggtgtcga tgcgtacgtt gccgttgtcg
     1681 tcggcctcac cggtcggctt gctgacttcc tggacgatgt cgaggaacac caggccatcg
     1741 cgggtcaccc gcggacgcac cttgaggatg atgccggtgt ccaggtactg caccgaactg
     1801 taggtattgt cgttgccgtt gcccgggttg aggctgaccg acgagatcgg gatgcgctgg
     1861 ccgacgttga ggctggcctc gcggttgttc tgggtgaaca ccgagggggc ggacagcgtg
     1921 cgcacgtcgg tgacgttgtc cagcgcgctg acgatcgccg ccgcattgtt gccgaggaag
     1981 gtccagctca gcaggttgcc gatggaaccg ccattgggga cattgggctg gctgatgcca
     2041 ccggaatagc tgccgaagct gttgcggccg gtcggatacg gaaaaccggc ttcgccgacc
     2101 gcgttatcga agaaccagct gacgccgtac tgcagcgcgc ccttcagcgc cacgctgatc
     2161 acctgcgcct cgatgtggac ctgcatcggc atcacgtcga tgcgctcaat cacttcgcgg
     2221 atcgatttcc actgcgatgg actggcgcgg accagcagcg agttggtgtc ttccaccgcg
     2281 gacacgccga cctcggcccc ttccaccttg agggtgacct tgccgttgcc gctgcggttg
     2341 gccggcaggt tgccgccatc gcccaggccg gtgttgaccg cggcgctgga ggtgccggac
     2401 ttgt
//
LOCUS       DAOOCX010000237         2390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_45589_length_2390_cov_5.181585, whole genome shotgun sequence.
ACCESSION   DAOOCX010000237 DAOOCX010000000
VERSION     DAOOCX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45589_length_2390_cov_5.181585"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PK399_12950"
     CDS             <1..200
                     /locus_tag="PK399_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14070.1"
                     /translation="QSLYATTINETARDWAGQVVYSYKVNPHTAVYAGASYGAFMDDD
                     NLELFGNTRSVFVKLSYGWQP"
     gene            292..1269
                     /locus_tag="PK399_12955"
     CDS             292..1269
                     /locus_tag="PK399_12955"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HPW14071.1"
                     /translation="MHRLHGFAIAGCLVTSLVLGCARQESAADAEASAGAPAVESAAA
                     DVARQPSAPEPDAAAIADATPTATQLTSSAATYTDAQRKFIRTARAEFQVKDVYRSAL
                     AIEDAVAAHGGFVVRNDANTQVGSVQRRPRGDGKLLELAEYTVRGTLVVRVPSERTQP
                     FLRSIVTQMEFLDARSFEARDAQFDLLRQQLAWRRNQDAQQDIGTAIEVGGKLGQKTD
                     AIAARSDTAAARDEALLAQKQFEDRVAFSTIELSLHQSPRIRQTELVDTESVFAQNGP
                     GFFARLGSALRAGWYGLLDMLLALLHVWPLWLALGAGAWAWRRLRRPRG"
     gene            complement(1282..1473)
                     /locus_tag="PK399_12960"
     CDS             complement(1282..1473)
                     /locus_tag="PK399_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14072.1"
                     /translation="MTDQKPLRSFRESPWRYSQFVVLGLIVAGLVKWLSPFGWLPSLA
                     VGAVVGVGYLLLEKKRGVI"
     gene            complement(1470..2321)
                     /locus_tag="PK399_12965"
     CDS             complement(1470..2321)
                     /locus_tag="PK399_12965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW14073.1"
                     /translation="MKLYAAMNRLFRRSYALKLFAVAFLGTHIPLLALLGWSIWRGWD
                     SDPLLVQVAVLCLFATLAGTALTLYLLHGLLAPVRASAAALDAFDTHAVLPQLPEDGH
                     DEAGLLMRGLNRSLRRIDDGIRELEWVARHDALTGALNRHGGNVQLAASIAGLAERAA
                     FTVAVLDMDALKQINDQHGHDAGDRALVRIVDGLRPLLGPNDWICRQGGDEFLLGLHD
                     DVRTLEVRLADWSAALVASDPLLRLSIGLAGYVPGEHAHALCRRADTAMYRAKRSGAG
                     ISMELPA"
BASE COUNT          408 a          843 c          788 g          351 t
ORIGIN      
        1 accagtcgct gtacgcgacc acgatcaacg agaccgcgcg cgactgggcc ggccaagtgg
       61 tctattcgta caaggtcaat ccgcacaccg cggtctacgc cggcgcgtcg tacggcgcgt
      121 tcatggacga cgacaacctg gagctgttcg gcaacacccg cagcgtgttc gtgaagttga
      181 gctacggctg gcagccgtaa ccccgttcaa tccgcctccg cgttgttagc atggccgccg
      241 gccgacccgc cggcgcgccc ctttcatcac ggatcgagga ttgtcatgcg catgcaccga
      301 ctccacggct ttgccatcgc cggctgcttg gtcaccagcc tcgtcctggg ctgcgccagg
      361 caggagtctg cggccgatgc tgaagcctcg gccggcgcgc cggcagtgga atccgcggcg
      421 gccgatgtcg ccaggcaacc atcggcacca gagcccgacg cggccgccat cgcagacgcc
      481 acgccgaccg cgacgcaact gacgtccagc gcggccacct acaccgacgc gcaacgcaag
      541 ttcatccgca ccgcgcgtgc cgagttccag gtcaaggacg tgtaccgctc tgccctggcg
      601 atcgaagatg cggtcgccgc gcatggaggc ttcgtggtca ggaacgatgc caatacccag
      661 gtcggttccg tgcaacgccg gccacgaggc gacggcaagc tgctggaact ggccgaatac
      721 acggtgcgcg gcacccttgt cgtacgcgtg cccagcgagc gcacccagcc cttcctgcgc
      781 tcgatcgtga cccagatgga attcctcgac gcccgcagct tcgaggcgcg cgatgcgcag
      841 ttcgacctgc tgcgacagca actggcctgg cgccgcaacc aggacgcgca gcaggacatc
      901 ggcaccgcga tcgaagtcgg cggcaagctg ggacagaaga cggacgccat cgccgcgcgc
      961 agcgacaccg cggcggcacg cgatgaagcg ttgctcgcgc agaagcagtt cgaggatcgc
     1021 gtcgcgttca gcaccatcga gctgtcgctg catcaatcgc cgcgcattcg ccagaccgag
     1081 ctggtcgaca ccgaatcggt gttcgcgcag aacggccccg gattcttcgc ccgccttgga
     1141 agcgcgttgc gcgcaggctg gtatggcctg ctggacatgc tgcttgccct gctgcatgtg
     1201 tggccgctgt ggctggcgct gggcgcgggt gcatgggcgt ggcgacgcct gcgccgacca
     1261 cggggatgag ggcagcgcgg ctcagatcac gccgcgcttc ttctcgagca gcaggtagcc
     1321 gacgccgacc accgcgccca ccgccagcga cggcagccac ccgaacgggc tgagccactt
     1381 caccagtcct gccacgatca ggcccagcac cacgaactgc gaatagcgcc atgggctttc
     1441 gcggaaactg cgcaggggtt tctggtcggt catgcgggga gctccatcga aatgccagca
     1501 ccgctgcgct tggcccggta catcgccgtg tcggcgcgcc ggcacagcgc gtgcgcatgt
     1561 tcacccggca cgtagccggc caggccgatg ctcaggcgca acaacggatc ggatgccacc
     1621 agcgccgccg accagtcggc caggcgcact tccagcgttc gcacgtcgtc gtgcaggccc
     1681 agcaggaatt cgtcgccgcc ctgccggcag atccagtcgt tcggccccag caacgggcgc
     1741 aggccatcga cgatccgcac cagggcgcgg tcgccggcat cgtggccatg ctggtcgttg
     1801 atctgcttga gcgcgtccat gtccagtacc gcgacggtga acgccgcccg ctcggccagg
     1861 cccgcgatcg acgcagccag ctgcacgttg ccgccgtgcc ggttcaacgc gccggtgagc
     1921 gcatcgtgcc gcgcgaccca ctcgagttca cggatgccgt cgtcgatccg gcgcaggctg
     1981 cgattgagcc cgcgcatcag caggccggct tcgtcgtgcc catcctcggg caattgcggc
     2041 agcaccgcgt gggtgtcgaa cgcatccagc gctgccgcag aggctcggac cggggcgagc
     2101 agtccatgca gcaggtacag cgtcagcgcg gtgccggcca gggtggcgaa caggcagagg
     2161 acggccacct gcaccagcag cggatcgctg tcccagccgc gccagatgct ccagccgagc
     2221 agggccagca gcgggatgtg cgtgcccagg aaggcgaccg cgaacagctt cagcgcatac
     2281 gagcggcgga acaaccggtt catcgccgcg tagagcttca tctcagatcc ggtagccggt
     2341 gtgcgcggcg acgataccgc cggtgaggtt cttgtactcg catcgggcga
//
LOCUS       DAOOCX010000238         2318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_47842_length_2318_cov_9.163058, whole genome shotgun sequence.
ACCESSION   DAOOCX010000238 DAOOCX010000000
VERSION     DAOOCX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47842_length_2318_cov_9.163058"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PK399_12970"
     CDS             complement(<1..857)
                     /locus_tag="PK399_12970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPW14074.1"
                     /translation="MPSTALAELRRVFGHDRFRGEQGEIVEQLIAGGDALVLMPTGGG
                     KSLCYQLPALLREGTGIVVSPLIALMQDQVEALRQLGVRAAFLNSSLDAQAAMQVERQ
                     LADGQLDLLYVAPERLLTPRFLSLLERSRIALFAIDEAHCVSQWGHDFRPEYRQLTLL
                     HERWPEVPRIALTATADAPTRREIVERLALEDARQFVSSFDRPNIRYTVVAKDDSRRQ
                     LLEFLDDHRGDSGIVYCLSRRKVDATAELLAGRGLRALPYHAGLDASVRAEHQRRFLR
                     EDGIVMVAT"
     gene            complement(980..1567)
                     /locus_tag="PK399_12975"
     CDS             complement(980..1567)
                     /locus_tag="PK399_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HPW14075.1"
                     /translation="MSKKHAHPKDAKPAKADKSASPVAPAAVPTAPHADGAPGIDIGI
                     QAKDRAKIADGLARFLSDAFTLYLKTHNFHWNVTGSMFNALHTMFEAQYTEQWNALDD
                     IAERIRALGFNAPGSYAEFIKLSSIPEEPGLTDTADWREMVRQLVAGNEAVCRTARKV
                     LDVADDADDAPTEDLLTQRLQTHEKYAWMLRSLLQ"
     gene            1663..>2318
                     /locus_tag="PK399_12980"
     CDS             1663..>2318
                     /locus_tag="PK399_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW14076.1"
                     /translation="MASTTPHSKPQSAPTPAQARRALDGALTRDRGRLLGLWAKWNAK
                     AGDDGLRQAFSTKLQASLAERERRAAALPAATVDASLPIAAHADDIVELIRKHQVVVL
                     AGETGSGKTTQLPKLCLLAGRGAAGMVGCTQPRRIAARSVARRVAEELQVQLGGAVGF
                     QVRFNDAVSEQTAIKFMTDGILLAEIQNDRWLSKYDTLIIDEAHERSLNIDFLLGYLK
                     "
BASE COUNT          372 a          767 c          818 g          361 t
ORIGIN      
        1 atcgtcgcca ccatcacgat gccatcctcg cgcaggaaac ggcgctggtg ttcggcgcgg
       61 acacttgcgt ccagccccgc gtggtagggc agcgcccgca ggccgcgccc ggccagcagc
      121 tccgcggttg catccacctt gcgccgcgac aggcaataga cgatgccgct gtcgccgcga
      181 tggtcgtcga ggaactccag cagctgccgc cggctgtcgt ccttggccac caccgtgtag
      241 cggatattgg gccggtcgaa ggagctgacg aactggcgcg cgtcttccaa tgccaggcgc
      301 tcgacgatct cgcggcgtgt cggcgcgtcc gcggtggcgg tcagcgcgat ccgcgggacc
      361 tccggccagc gttcgtgcag caacgtgagc tggcggtact cggggcggaa atcgtgcccc
      421 cattgcgaca cgcagtgcgc ctcgtcgatg gcgaacagcg cgatccggct gcgctcgagc
      481 agcgacagga agcgcggggt cagcaggcgt tcgggggcga cgtagagcag gtcgagctgg
      541 ccatcggcca attgccgttc gacctgcatc gccgcctgcg catccaggct ggagttcagg
      601 aacgcggcac gcacgccgag ctggcgcagc gcttcgacct ggtcctgcat caacgcaatc
      661 agcggcgaga ccacgatgcc ggtgccctcg cgcaacagcg cgggcagttg gtagcaaagc
      721 gacttgccgc caccggtcgg catcagcacc agggcgtcac cgcctgcgat cagctgttcg
      781 acgatctcgc cctgttcgcc gcggaaacgg tcgtggccga agacgcggcg gagttcggca
      841 agggcggtgg acggcatgcg catagggtaa caagcgaggc gatcctgggc gcctgcgatg
      901 atcggcgccg cgccgcgatc ccgcacgaaa aaagagccgg caccttggcg gcaccggccc
      961 gtcggggggt gcggacgcgt tactgcagca gcgagcgcaa catccaggcg tacttctcgt
     1021 gggtctgcag gcgctgggtc aggaggtcct cggtgggcgc gtcgtccgca tcgtcggcca
     1081 cgtccagcac cttgcgcgcg gtgcggcaca ccgcttcgtt gccggccacc aactggcgga
     1141 ccatctcacg ccagtccgcg gtgtcggtca ggccgggctc ctccgggatc gagctcagct
     1201 tgatgaactc ggcataggaa ccgggggcat tgaagcccag cgcgcggatg cgctcggcga
     1261 tgtcgtccag cgcgttccac tgctccgtgt actgcgcctc gaacatcgtg tgcagggcgt
     1321 tgaacatcga cccggtgacg ttccagtgga agttgtgggt cttcaggtag agcgtgaacg
     1381 catccgacag gaagcgcgcc aggccgtcgg cgatcttcgc gcggtccttc gcctggatgc
     1441 cgatgtcgat gccgggcgcg ccatccgcat gcggggcggt cggaacggcg gcaggcgcga
     1501 cgggactggc ggacttgtcg gctttcgccg gcttcgcatc cttggggtgg gcatgcttct
     1561 tggacatggc gtggcctcat gcgggtgttg ttgggggact tgcggcgaca atacgcgccc
     1621 tggatgtttc cagaccatga aaatccgcag cgcaaccgag tcatggcgtc gaccactccg
     1681 cattccaagc cgcaatccgc gccgaccccg gcgcaggccc gtcgcgccct cgatggcgcg
     1741 ctgacccgcg accgtggccg cctgctgggc ctgtgggcga agtggaacgc gaaggccggc
     1801 gacgacggct tgcgccaggc gttcagcacg aagctgcagg cgtcgttggc cgagcgcgag
     1861 cgccgcgcgg cggcattgcc tgccgccacg gtggatgcat cgctgccgat cgccgcgcac
     1921 gcggacgaca tcgtcgagct gatccgcaag caccaggtcg tggtgctggc cggcgaaacc
     1981 ggcagcggca agaccacgca gttgcccaag ctctgcctgc tggcggggcg cggtgccgcc
     2041 ggcatggtcg gctgcaccca accgcggcgg atcgcggcgc gctcggtggc gcggcgggtg
     2101 gcggaggaat tgcaggtgca gctgggcggc gcggtcggct tccaggtccg cttcaacgat
     2161 gccgtgagtg aacagaccgc gatcaagttc atgaccgacg gcatcctgct ggcggaaatc
     2221 cagaacgacc gctggctgtc gaaatacgac accctgatca tcgacgaggc gcacgagcgc
     2281 agcctcaaca tcgacttcct gctgggctac ctcaagca
//
LOCUS       DAOOCX010000239         2315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_47940_length_2315_cov_5.013717, whole genome shotgun sequence.
ACCESSION   DAOOCX010000239 DAOOCX010000000
VERSION     DAOOCX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47940_length_2315_cov_5.013717"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..770)
                     /locus_tag="PK399_12985"
     CDS             complement(159..770)
                     /locus_tag="PK399_12985"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPW14077.1"
                     /translation="MPAPPILRDPLPYADALSSREPAGIDTVVIHCTELPDLATAREF
                     GERILYPGSGTGNSGHYYIDRDGGIVEYVPPGRIAHHTRGWNPRSIGIELVNIGRYPD
                     WLDSRRQAMDEPYTDAQVDALVGLLGDLQARIPSLVQIAGHEDLDLDEVEASDDPGVK
                     VRRKRDPGPLFPWQRVLQAVTLQRFTLDRPQAGIDGKTAGRLA"
     gene            793..1287
                     /locus_tag="PK399_12990"
     CDS             793..1287
                     /locus_tag="PK399_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HPW14078.1"
                     /translation="MKQPPPDSELGRLFSQFPRAGQVEWIGLRPARDVPMRPVDAAEA
                     AAGKGLHGDRYASGSGKRGITLIQAEHLPVIAALSGHPEISPATLRRNVVVSGIPLVA
                     LKGRRFRIGDVLLEGTDPCDPCSRMEAALGPGGYNAMRGMGGLCARILEGGSVRLGDA
                     VVAE"
     gene            1284..1808
                     /locus_tag="PK399_12995"
     CDS             1284..1808
                     /locus_tag="PK399_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14079.1"
                     /translation="MIGHCILSHGFESGPDATKVTALADAAERLGWTHERPDYTDLDA
                     RREVSELGDVAARLQRLVDLAKHAATRGPLVLAGSSLGAWISGQVSLQVPTAGLFLMA
                     PPIAMGRAPALQAAAVPTSIIHGWHDELIPAVQVVDWAAARNARLLLVDDSHRLSGHV
                     QASAEAFAQLLASL"
     gene            1859..>2315
                     /locus_tag="PK399_13000"
     CDS             1859..>2315
                     /locus_tag="PK399_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HPW14080.1"
                     /translation="MKFFASCGKGLEYLLADELVALGCTRATATMAGANVEGSLLDAQ
                     RAVLWSRLASRVAWPIAEFECLDEHALYAGAAAVDWTQHLGAGHSIAIDAHVSGDAIT
                     HARYAAQRVKDAIVDTLRASTGTRPDVDVETPDVRISLVIRKGRAILSID"
BASE COUNT          334 a          763 c          836 g          382 t
ORIGIN      
        1 ggccgcggaa caggttgtct tccagccgct ccagcgagag cagttcgatc agttctgaga
       61 cgggaggagt catgccgcga ttataggtgg cggccgaggt cgggcggttc cgtcgcttga
      121 cggcacctgt tgactcgggt ctcccacgcg gctccccgtt acgcgagtct gccggcggtc
      181 ttgccgtcga tgccagcctg tgggcgatcc agggtgaagc gttgcagcgt cactgcctgc
      241 agcacccgct gccagggaaa cagcggcccc gggtcgcgct tgcggcggac tttaacgccc
      301 ggatcgtcgc tggcttcgac ctcgtccagg tccaggtcct cgtggccggc gatctgcacc
      361 agggacggga tgcgtgcctg caggtcgcca agcaggccga ccagggcgtc gacctgcgcg
      421 tcggtatagg gctcgtccat cgcctgtcgg cgcgaatcca gccagtccgg gtagcggccg
      481 atgttgacca gttcgatgcc gatgctgcgc ggattccagc cccgggtgtg gtgggcgatg
      541 cggccgggcg ggacgtattc cacgatgccg ccgtcgcggt cgatgtagta atgaccgctg
      601 ttgccggtgc cgctgccggg atacaggatc cgttcgccga actcgcgcgc ggtggccagg
      661 tcaggcagct cggtgcagtg gatcaccacg gtatcgatgc cggctggttc gcgcgatgac
      721 agggcatcgg catagggcag gggatcgcgc aggatcgggg gcgctggcat cgcccgatgc
      781 tagcattcgc gcatgaagca accgcctccc gattccgagc tcggccgcct gttctcgcag
      841 ttcccgcgcg cgggccaggt cgaatggatc ggtttgcgtc cggcgcgcga cgtgccgatg
      901 cggccggtcg atgccgccga ggctgccgca ggcaagggac tgcacggcga ccgctacgcc
      961 agtggcagcg gcaagcgtgg catcaccctg atccaggccg agcacctgcc ggtgatcgcc
     1021 gcgctctcgg ggcacccgga aatctcgcct gcaaccctgc gccgcaacgt ggtggtctcg
     1081 ggcatcccgc tggtcgcgct gaaagggcgg cgtttccgca ttggcgatgt gctgctggaa
     1141 ggcaccgatc cgtgcgatcc gtgttcgcgg atggaagccg cgctcgggcc cggcgggtac
     1201 aacgcgatgc gcggcatggg cgggttgtgc gcgcgcatcc tcgaaggcgg gagcgtgcgc
     1261 ctgggcgatg cggtggtggc cgaatgatcg ggcactgcat cctctcgcac gggttcgaga
     1321 gcggccccga cgcgaccaag gtcaccgcgc tggcggatgc cgccgagcgc ctgggctgga
     1381 cccacgagcg cccggattac accgacctgg atgccaggcg cgaggtcagc gaactgggcg
     1441 atgtcgcggc gcgactgcag cgcctggtgg acctggcgaa gcatgccgcc acgcgtggtc
     1501 cgctggtgct ggccggttcc agcctgggcg cgtggatttc cgggcaggtg tcgctgcagg
     1561 tgccgacggc aggcctgttc ctgatggcgc cgccgatcgc gatgggccgc gcccccgcgc
     1621 tgcaggcagc cgcggtgccg acctcgatca tccatggttg gcacgacgaa ctgatccccg
     1681 cggtgcaggt ggtcgactgg gccgccgcgc gcaacgcgcg cctgctgctg gtcgacgact
     1741 cgcaccgcct gtccggccat gtgcaggcga gtgcagaggc gttcgcacaa ttgctcgcga
     1801 gtctttgacg tcgttccggc gcaggccggg acgaccgtaa caatccgatg aggtcgcttt
     1861 gaaattcttc gcatcctgcg gcaagggcct ggagtacctg ctggccgacg aactggtcgc
     1921 actgggttgt acccgcgcca ccgccacgat ggcgggtgcc aacgtggaag gcagcctgct
     1981 cgacgcgcag cgcgcggtgc tgtggtcgcg cctggccagc cgcgtggcct ggccgatcgc
     2041 cgagttcgag tgcctggacg aacacgcgct gtacgccggt gccgcagcgg tggactggac
     2101 ccagcatctc ggcgcgggac acagcatcgc catcgatgcg cacgtgtccg gcgatgccat
     2161 cacccacgcg cgttatgcgg cgcagcgggt gaaggacgcc atcgtcgata ccctgcgcgc
     2221 gagcaccggc acgcgccccg atgtcgatgt cgaaacgccc gatgtccgca tcagcctggt
     2281 gatccgcaag ggtcgcgcga tcctgtcgat cgacc
//
LOCUS       DAOOCX010000240         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_48396_length_2301_cov_10.742654, whole genome shotgun
            sequence.
ACCESSION   DAOOCX010000240 DAOOCX010000000
VERSION     DAOOCX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48396_length_2301_cov_10.742654"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PK399_13005"
     CDS             <1..642
                     /locus_tag="PK399_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HPW14081.1"
                     /translation="DFVFEDGFGYERYVDYLLDVPMYFSYRNGEYIDASGKSFRTFLR
                     GELDVLPGALPTIRDWNDHMTTAFPEVRLKKFLEMRGADGGPWNRLCALPAFWVGLLY
                     DDAALDAAWDLVKDFSMAERNALRDGVPTHALKTPFRNGTLRDIAIEALKIAQAGLRR
                     RAKADACGTDESGFLEPLMDIVDANETPAERKLALFHGEWNGSVDPVFREFAY"
     gene            complement(644..>2301)
                     /locus_tag="PK399_13010"
     CDS             complement(644..>2301)
                     /locus_tag="PK399_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14082.1"
                     /translation="PMPRLLLLALACLVAGCGTPTPKPPPAASASAPPLFDDLGDLHR
                     DIGTPVPAAQRYFDQGLRMAYGFNHEAARRAFAEAERLDPRCAMCAWGQALVLGPNIN
                     LPMDPLLAAQATELATRAVALSANARPADRALIEALQLRYTNPAPQDRATLDAAYAEA
                     MGKVVARFPGDDDAATLFAEALMDLTPWAYWTAAGQQTTHTPRILAALERVLARNPRH
                     IGAMHYYIHATEASPDPGRAEPYADALAALAPGAGHLVHMPAHTYIRMGRYHDATLVN
                     FAAANADKAFLSFCRGSNGVYPLGYVPHNWHFATMTTGLTGSRTLALQAAEQTAQRAD
                     RALMGAPPLEFMQLFVVTPLLTQVRFGEWDAILDEAAPTDEPPFVAAIRHFARGMAHA
                     RAGRLDPAARDAEALHAIAVDPATARVAFGMNNADAVLRVADPLLRGELLRARGRQAD
                     ALAALREAAAAEDTLNYNEPADWPLPVRWYLGAALLDAGDPAGAAQAYRQDLQAYPGN
                     GWSLFGLAKAQRALGDQAGAADSERRLAQAWQWADAPLVASRY"
BASE COUNT          328 a          764 c          845 g          364 t
ORIGIN      
        1 gacttcgtgt tcgaggacgg cttcggctac gagcgctacg tcgattacct gctcgacgtg
       61 ccgatgtatt tctcctaccg caacggcgaa tacatcgacg ccagcggcaa gtcgttccgc
      121 acgttcctgc gtggcgagct cgacgtgctg cccggcgcgt tgccgaccat ccgcgactgg
      181 aacgaccaca tgaccaccgc cttcccggaa gtgcggctga agaaattcct ggagatgcgc
      241 ggtgccgatg gcgggccgtg gaaccgcctg tgcgcgctgc cggcgttctg ggtcggcctg
      301 ctgtacgacg atgccgcgct ggatgcggca tgggacctgg tcaaggattt ctcgatggcc
      361 gagcgcaacg cgttgcgcga tggcgtgccg acgcatgcgc tgaagacccc gttccgcaac
      421 ggcacgctgc gcgacatcgc catcgaggcc ttgaagatcg cccaggccgg tttgcgccgc
      481 cgggccaagg cggatgcctg cggtaccgac gaatccggct tcctggaacc gttgatggac
      541 atcgtcgacg ccaacgaaac ccccgccgag cgcaagctcg cgttgttcca tggcgaatgg
      601 aacggttcgg tggatccggt gttccgcgag ttcgcgtact gagtcagtag cgcgaggcca
      661 ccagcggcgc atccgcccac tgccaggcct gcgccaggcg acgctcgctg tcggccgcgc
      721 cggcctggtc gccgagcgcg cgttgcgcct tggccagccc gaacagcgac cagccattgc
      781 ccggataggc ctgcaggtcc tgtcgatagg cctgcgccgc gccggcagga tcgccggcgt
      841 cgagcagggc ggcgcccagg taccagcgca ccggcaatgg ccagtccgcc ggttcgttgt
      901 agttgagcgt gtcttcggcg gcggccgcct cgcgcagtgc ggccagcgca tcggcctggc
      961 gtccgcgtgc ccgcagcagt tcgccgcgca gcagcggatc ggcgacccgc agcaccgcgt
     1021 cggcgttgtt catgccgaag gcgacccgcg ccgtcgccgg gtcgactgcg atcgcatgca
     1081 gcgcctccgc atcgcgcgcc gccgggtcga gcctgcctgc gcgcgcatgc gccatgccgc
     1141 gcgcgaaatg gcggatcgcg gccacgaagg gcggctcatc cgttggcgca gcctcgtcca
     1201 ggatcgcgtc ccattcgccg aagcggactt gcgtgagcag tggcgtgacc acgaacagtt
     1261 gcatgaactc cagcggcggc gcgcccatca gcgcgcggtc ggcgcgctgc gcagtttgtt
     1321 cggcggcctg tagcgccaac gtgcgcgagc cggtcaggcc ggtggtcatg gtcgcgaagt
     1381 gccagttgtg cggcacgtag cccagcgggt acacgccatt gctgccgcgg cagaacgaga
     1441 ggaaggcctt gtcggcattc gccgcggcga aattgaccag ggtggcgtcg tggtagcggc
     1501 ccatgcggat gtaggtgtgc gcgggcatgt gcaccaggtg gccggcgccg ggtgccaggg
     1561 cggccagcgc atctgcatac ggttcggcgc ggcccgggtc cggcgacgcc tcggtggcgt
     1621 ggatgtagta gtgcatcgcg ccgatgtggc ggggattgcg tgcgagcacg cgttcgagtg
     1681 cagcgaggat gcgcggtgtg tgggtggtct gctggccggc cgcggtccag taggcccacg
     1741 gggtcaggtc catcagcgcc tcggcgaaca gggtcgcggc gtcgtcgtcc cccgggaagc
     1801 gcgccacgac cttgcccatc gcctcggcgt aggcggcgtc cagcgtcgcg cggtcttgcg
     1861 gcgcggggtt cgtgtagcgc agttgcagcg cctcgatcag cgcacggtcc gcaggtcttg
     1921 cattggccga caacgcgacc gcgcgcgtgg ccagttcggt cgcctgcgcg gccagcagcg
     1981 gatccatcgg caggttgatg ttgggcccga gcaccagcgc ctgcccccac gcgcacatcg
     2041 cgcagcgcgg atcgagccgt tcggcttcgg cgaaggcgcg ccgcgcggct tcgtggttga
     2101 agccgtaggc catgcgcagg ccttggtcga aatagcgttg cgcggcgggg accggcgtgc
     2161 cgatgtcgcg atgcaggtcg ccgagatcgt cgaacagcgg tggcgcggac gccgaggccg
     2221 ccggtggtgg tttcggcgtg ggcgtgccgc accctgcgac caggcaggca agggcaagca
     2281 acagcaacct gggcatgggc g
//
LOCUS       DAOOCX010000241         2230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_50783_length_2230_cov_4.175632, whole genome shotgun sequence.
ACCESSION   DAOOCX010000241 DAOOCX010000000
VERSION     DAOOCX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50783_length_2230_cov_4.175632"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PK399_13015"
     CDS             complement(<1..769)
                     /locus_tag="PK399_13015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPW14083.1"
                     /translation="MNRIALLLVCVLAWLPANSAAAEKPGEASIVFVGDVMVAETPGE
                     LIAKGVDPFLPFQPILRAHDIRIGNLECTVATGGKAEAKPYTFRADPAVLPLLARHFD
                     AMSLANNHSGDFGKAAFAEQLGLMKKAGLPYFGGGHDATEAHAPLIVERNGLRIALLG
                     YLEFKPRSFEADAHRPGVAWSGEDDQVIEDIIAARTIHHADIVIPYMHWGWEEEAQPS
                     PRLREFARRMIDAGADMVVGGHPHVTQGAETYKGKPII"
     gene            complement(831..1502)
                     /locus_tag="PK399_13020"
     CDS             complement(831..1502)
                     /locus_tag="PK399_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14084.1"
                     /translation="MARPPGWIVLMVLGCLVLGWWLSNPAPSLPPPATGVASVAGGAR
                     ADTSACPLPPLVARGDAPLQSEVPSSMPSLPLEGATLQPLAGFSVEARVLGREDYRMG
                     READFSPMDLALGWGRMTDDAVLGQLDISQGGRWYSYRWQRDPPIPVDEIVRSSANMH
                     MVPADDAVAEALRDVDAGDRVRIDGWLVQVNTPDGWRWRSSLTREDSGGGACEVVYVC
                     AVSQL"
     gene            complement(1514..>2230)
                     /locus_tag="PK399_13025"
     CDS             complement(1514..>2230)
                     /locus_tag="PK399_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HPW14085.1"
                     /translation="VVQRARRIVADVEQMREAARRSKDPESGSLRLGVFPTLAPYLLP
                     HVVPQLRERFPQLELLLVEEKSDVLLQRLREGRLDAALLALPVHDEQLHAEFLFEEPF
                     VLAAPQGHELATRTSLHVDELSDETLLLLEDGHCLRDQALDVCRLSGAQEKSGFRATS
                     LETLRQMVAAGVGVTLLPALSVHAPIVQPANIRLVKFSEPAPSRRIALVWRKSSAMEA
                     FLQQLAAEVGGLARAQLQSH"
BASE COUNT          357 a          773 c          742 g          358 t
ORIGIN      
        1 agatgatcgg cttgcccttg taggtctccg cgccctgggt cacgtgcgga tggccgccca
       61 cgaccatgtc ggcgccggcg tcgatcatcc gccgcgcgaa ttcgcgcagg cgcgggctgg
      121 gttgggcttc ctcttcccaa ccccagtgca tgtaggggat cacgatgtcc gcgtggtgga
      181 tggtgcgcgc cgcgatgatg tcctcgatga cctggtcgtc ctctccgctc catgccacgc
      241 ccgggcggtg ggcatcggct tcgaacgaac gcggcttgaa ctccaggtag ccgagcaggg
      301 cgatgcgcag gccattgcgt tccacgatca acggcgcgtg cgcttcggtc gcgtcgtggc
      361 cgccgccgaa atacggcagc cctgcctttt tcatcagccc gagttgttcc gcgaacgcgg
      421 ccttgccgaa atcgccggag tggttgttgg ccagcgacat cgcgtcgaaa tgtcgcgcca
      481 gcagcggcag cacggccgga tccgcgcgga aggtgtaggg cttggcctcg gccttgccgc
      541 cggttgccac cgtgcattcc aggttgccta tgcggatgtc gtgcgcccgg aggatgggct
      601 ggaagggcag gaacggatcg acaccctttg cgatgagctc gccaggggtt tccgcgacca
      661 tgacgtcgcc gacgaagacg atgctggcct cgcccggctt ctcggccgca gccgaatttg
      721 ccggcagcca cgcgagcacg cacaccaaca acaatgcgat tctattcatg ctggctatca
      781 tcgcgcaaaa cgcggccacg catgcagcgt ccatgaccgc cgccgccgac tcagagttgg
      841 cttaccgcgc agacgtatac gacttcgcag gcgccaccgc cgctgtcctc gcgggtcagt
      901 gaactccgcc agcgccagcc gtccggcgtg ttgacctgca ccagccagcc gtcgatgcgc
      961 acgcggtcgc ccgcgtcgac atcgcgcagg gcctctgcca ccgcgtcgtc ggccggcacc
     1021 atgtgcatgt tggcgctgct gcgcacgatc tcgtccaccg ggatgggcgg gtcgcgttgc
     1081 cagcggtagc tgtaccagcg accgccctgg ctgatgtcga gctgtcccag caccgcgtcg
     1141 tcggtcatcc ggccccagcc cagggccagg tccatcggcg agaagtcggc ctcgcggccc
     1201 atgcggtaat cctcgcgacc aagcacgcgc gcctccacgc tgaaaccggc caacggctgc
     1261 agcgtggcgc cttccagcgg cagcgacggc atcgacgacg ggacctcgct ttgcagtggc
     1321 gcgtcgccgc gcgcgaccag tggcggcaac gggcatgcgc tggtgtccgc gcgcgcgccg
     1381 ccggcgacgg atgcgacacc tgtcgcgggc ggcggcaacg atggcgcggg attggacagc
     1441 caccagccca gtacgaggca acccaacacc atcaacacga tccagcccgg cggtcgtgcc
     1501 atcgatggtt tcctcagtgg gactgcaact gcgcgcgggc aagtccgccg acttcagcgg
     1561 ccaactgctg caggaacgct tccattgccg atgacttgcg ccagaccagg gcgatgcggc
     1621 ggctcggcgc gggttcgctg aacttcacca ggcggatgtt ggcaggctgc acgatcggcg
     1681 cgtgtaccga cagcgccggc aacaaggtca cgccgacccc ggccgcgacc atttgccgca
     1741 aggtctccag gctggtggcg cggaaaccgg acttctcctg cgcgccggac agccggcaga
     1801 cgtcgagcgc ctggtcgcgc aggcagtggc cgtcttccag caacagcagt gtttcgtcgg
     1861 agagttcgtc gacatgcaac gacgtgcgcg tcgcgagttc atgcccctgc ggcgcggcca
     1921 gcacgaaggg ttcctcgaac aggaactctg cgtgcaactg ctcgtcgtgc acgggcaatg
     1981 cgagcagcgc ggcatccagt cgcccttcgc gcaggcgctg cagcaggacg tcactcttct
     2041 cctcgaccag cagcagttcc agttgcggga aacgctcgcg tagctgcggc accacatgcg
     2101 gcagcaggta gggagcaagt gtcgggaaca cgccgagccg cagcgagccg gactccggat
     2161 ccttgctgcg gcgggcggct tcgcgcatct gctcgacgtc ggccacgatg cggcgcgcgc
     2221 gctgcaccac
//
LOCUS       DAOOCX010000242         2030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_58996_length_2030_cov_8.572152, whole genome shotgun sequence.
ACCESSION   DAOOCX010000242 DAOOCX010000000
VERSION     DAOOCX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58996_length_2030_cov_8.572152"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2030
                     /gene="polA"
                     /locus_tag="PK399_13030"
     CDS             <1..>2030
                     /gene="polA"
                     /locus_tag="PK399_13030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPW14086.1"
                     /translation="AAGEPTGALFGIVNMLRGHLKEKPDYIAFVMDAPGRTFRDDLYA
                     DYKAHRPPMPDELRAQVEPMMAIVAALGLPVLRVDGVEADDVIGTLAVRGAEAGIDVT
                     VSSGDKDMAQLVRPHVALVNTMSGGKLDSDEAVMAKFGVRADQIVDYLALMGDAIDNI
                     PGIPKCGPKTAAKWLAEYGSLDGVIANADKVGGKIGEALREALPRLPLNRVLTTIKTD
                     VALDQAPAELHLRERDVEALRALYTRYGFNQALKELDGSASSNASPPALEKEPGIARG
                     NGFVAPGASASQAGLDPVLSAPGEYECVLGMAQLDAWVARLQAAGEFAFDTETDSLDP
                     MRANLVGISLSVEPGKACYIPIAHEAPGVAAQLPRDVVLDALRPLLADPAKKKLGQHG
                     KYDLHVLRRHGVDVHGYADDTMLESFVLNAGIARHDMDSLAQRHLGYQTVKYEDVAGK
                     GAKQIGFAQVALDDATRYAAEDADITLRLHRAMLPKLQAEPALLSVYRDIEMPLVPVL
                     ARVEANGVMVDAEELRRQSADLSRRMLAAQQKATELAGRSFNLDSPKQLGALLFDELK
                     LPALVKTPSGAPSTNEEALEAIADQHELPRIILEYRGLAKLRSTYTDKLPEMVNPDTG
                     RVHTSYHQAGAATGRLSSSDPNLQNIPIRSEDGRRIRTAFVAPPGRKIVACD"
BASE COUNT          365 a          671 c          697 g          297 t
ORIGIN      
        1 gcggccggcg agccgaccgg tgcgctgttc ggcatcgtca acatgctgcg cgggcacctg
       61 aaggaaaagc cggactacat cgccttcgtg atggatgcgc ccggcaggac gttccgcgac
      121 gacctctacg cagactacaa ggcccaccgt ccgccgatgc cggacgagct gcgcgcgcag
      181 gtcgaaccga tgatggcgat cgtcgccgcc cttggcctgc ccgtgctgcg cgtggatggc
      241 gtggaagccg acgacgtgat cggtaccttg gcggtgcgcg gcgccgaagc cggcatcgat
      301 gtcaccgtgt cgtccggcga caaggacatg gcgcaactgg tgcgtccgca cgtggcgctg
      361 gtcaacacca tgagcggcgg caagctcgat tccgacgaag cggtgatggc gaaattcggg
      421 gtgcgcgccg accagatcgt cgattacctg gcgttgatgg gcgatgcgat cgacaacatc
      481 cccggcatcc cgaaatgcgg gccaaaaacc gcggcgaagt ggctggccga atacggctcg
      541 ctggatggcg tgatcgcaaa cgccgacaag gtcggcggca agatcggcga ggccctgcgc
      601 gaagcgctgc cgcggctgcc actcaatcgc gtgctgacca ccatcaagac cgacgtggcg
      661 ctggaccagg ccccggccga gctgcacctg cgcgagcgcg atgtcgaggc gttgcgcgcg
      721 ttgtacacgc gctacggctt caaccaggcg ctgaaggaac tcgacgggag cgcatcgtcg
      781 aatgcgtcac cgccggcgtt ggagaaggag ccgggcatcg cgcgcggcaa cggtttcgtc
      841 gcgccgggcg cgtcggcatc gcaggcggga ctcgatccgg tgctgtccgc gcccggcgaa
      901 tacgaatgcg tcctcggcat ggcgcagctc gatgcatggg tggccaggct gcaggcggct
      961 ggggaattcg ccttcgacac cgagaccgat tcgctggacc cgatgcgcgc caacctcgtc
     1021 ggcatcagcc tgagcgtgga acccggcaag gcctgttaca tcccgatcgc gcatgaagcg
     1081 ccgggcgtgg ccgcgcaatt gccgcgcgac gtggtgctcg atgcgttgcg cccgctgctg
     1141 gcggatccgg cgaagaagaa actcggccag cacggcaagt acgacctgca cgtgttgcgt
     1201 cgccacggcg tcgatgtcca tggctatgcc gacgacacga tgctggaaag cttcgtgctg
     1261 aacgccggca tcgcccgcca cgacatggac tccctggcgc agcgccatct cggctaccag
     1321 accgtgaagt acgaggacgt ggccggcaag ggcgcgaagc agatcggctt cgcccaggtc
     1381 gcgctggacg atgccacccg ctatgcggcg gaagacgccg acatcacctt gcgcctgcat
     1441 cgcgcgatgc tgcccaagct gcaggccgag ccggcgttgc tgtcggtgta ccgcgacatc
     1501 gagatgccgc tggtgccggt gctggcacgg gtcgaagcca atggggtgat ggtcgatgcc
     1561 gaggaactgc gccggcagtc cgccgacctg tcgcggcgga tgttggccgc gcaacagaag
     1621 gccaccgaac tggccgggcg cagcttcaac ctggattcgc ccaagcagct cggcgcgctg
     1681 ctgttcgacg agctcaagtt gccagcgctg gtgaagaccc cgagcggcgc gccctcgacc
     1741 aacgaggaag cgctggaggc gatcgccgac cagcacgaac tgccgaggat catcctcgag
     1801 taccgcgggc tggcgaaact gcgcagcacc tataccgaca agctgccgga gatggtcaat
     1861 ccggacaccg gccgcgtgca caccagttac caccaggccg gtgctgcgac cgggcgcttg
     1921 tcgtcctccg atccgaacct gcagaacatc ccgatccgca gcgaggatgg acgacgcatc
     1981 cgcaccgcct tcgtcgcgcc gccggggcgc aagatcgtgg cctgcgacta
//
LOCUS       DAOOCX010000243         1482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_4007
            NODE_99504_length_1482_cov_3.273301, whole genome shotgun sequence.
ACCESSION   DAOOCX010000243 DAOOCX010000000
VERSION     DAOOCX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865978
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:44:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,608
            CDSs (total)                      :: 2,571
            Genes (coding)                    :: 2,547
            CDSs (with protein)               :: 2,547
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99504_length_1482_cov_3.273301"
                     /isolate="AMR_MDS_4007"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PK399_13035"
     CDS             complement(<1..769)
                     /locus_tag="PK399_13035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPW14087.1"
                     /translation="MKRLFLTCALAWPLAAVAQSDASTREDVSIVFVGDVMVAETPGE
                     LIAKRVDPFLPFQPLLKAHDIRIGNLECTVATGGKAEAKPYTFRADPAVLPLLARHFD
                     AMSLANNHSGDFGKAAFAEQLGLMKQAGLPYFGGGVDATEAHTPWIVERNGLRIALLG
                     YLEYKPRSFEADAHRPGVAWSGEDDRVIEDIIAARTVHHADIVIPYMHWGWEEEAQPS
                     PRLREFARRMIDAGADMVVGGHPHVTQGAETYKGKPII"
     gene            complement(819..>1482)
                     /locus_tag="PK399_13040"
     CDS             complement(819..>1482)
                     /locus_tag="PK399_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW14088.1"
                     /translation="MPRRWIVIAVLGCLLLGWWLSNPRSSLPAAATVAGAVETTSADT
                     SACPLPPRVEPGAPPLQSEVPSSMPPLKTEGATLQPLAGFSVEARVLGREDYRMGREA
                     EFSPTDLALGWGRMTEDAVLAQLQISQGGRWYRYRWKHDPPIPLGEIVRSSANMHMVP
                     ADDEVAAALRDVDAGERVRIDGWLVQVNTPDGWRWRSSLTREDTGGGACEVVYVCAVS
                     TY"
BASE COUNT          222 a          512 c          507 g          241 t
ORIGIN      
        1 agatgatcgg cttgcccttg taggtctccg cgccctgggt cacgtgcgga tggccaccga
       61 ccaccatgtc ggcgccggcg tcgatcatcc gccgggcgaa ttcgcgcagg cgcgggctcg
      121 gttgggcttc ctcttcccag ccccagtgca tgtacgggat cacgatgtcg gcgtggtgca
      181 ccgtgcgcgc ggcgatgatg tcctcgatca cccggtcgtc ctcgccgctc catgccacgc
      241 cgggacgatg ggcatcggcc tcgaacgaac gcggcttgta ttccaggtag ccgagcaggg
      301 cgatgcgcag tccgttgcgc tcgacgatcc acggcgtgtg cgcttcggtg gcatcaacgc
      361 cgccgccgaa gtaaggcagc ccggcctgtt tcatcagccc cagttgctcc gcgaacgccg
      421 ccttgccgaa gtcgccggaa tggttgttgg ccagcgacat cgcgtcgaaa tggcgcgcca
      481 gcagtggcag cacggccggg tccgcgcgga aggtgtaggg cttggcctcg gccttgccgc
      541 cggtggctac ggtgcattcc aggttgccga tgcggatgtc atgcgccttc aggaggggct
      601 ggaacgggag gaacgggtcc acacgcttgg cgatgagctc accgggggtt tcggcgacca
      661 tcacgtcgcc gacgaagacg atgctgacgt cttccctggt gcttgcgtcg gactgcgcca
      721 cggcggcgag gggccatgcc agcgcacagg tcaggaagag tcgcttcatg ccggccatca
      781 tcccgcgaat cggccctgcg cgcgacggga tcgtccgctc agtacgtgct gaccgcgcag
      841 acgtagacca cttcgcaggc tccgccgccg gtgtcctcgc gggtcagcga gctgcgccag
      901 cgccagccat cgggcgtgtt gacctgcacc agccagccgt cgatgcgcac gcgctcgccg
      961 gcatcgacat cgcgcagggc cgcggcgact tcatcatcgg cgggcaccat gtgcatgttg
     1021 gcgctgctgc gcacgatctc gcccagcggg atcggtggat cgtgtttcca gcggtagcgg
     1081 taccagcggc cgccctggct gatctgcagt tgcgccaaca ccgcatcctc ggtcatccgc
     1141 ccccagccca gcgccaggtc ggtgggcgag aactcggcct cgcggcccat gcggtagtcc
     1201 tcacggccga gcacgcgtgc ctcgacgctg aaacccgcaa gcggctgcag ggtcgcgcct
     1261 tccgtcttca acggcggcat cgacgacggc acctcgctct gcagcggcgg ggcacctggc
     1321 tccacccgag gtggcagcgg gcaagcgctg gtgtcggcac tggtggtctc gacagcaccg
     1381 gccaccgtcg cggctgcggg cagggaggag cgtgggttcg acaaccacca tcccagcagc
     1441 aggcagccga gtaccgcgat cacgatccag cgccgcggca tc
//
